LOCUS       DACEQH010000001       100825 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.001, whole genome shotgun sequence.
ACCESSION   DACEQH010000001 DACEQH010000000
VERSION     DACEQH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 100825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.001"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(550..2163)
                     /locus_tag="IW833_000001"
     CDS             complement(550..2163)
                     /locus_tag="IW833_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ1306154.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2304..3212)
                     /locus_tag="IW833_000002"
     CDS             complement(2304..3212)
                     /locus_tag="IW833_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1306155.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3395..4519
                     /locus_tag="IW833_000003"
     CDS             3395..4519
                     /locus_tag="IW833_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1306156.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4746..5663
                     /locus_tag="IW833_000004"
     CDS             4746..5663
                     /locus_tag="IW833_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1306157.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5656..6489
                     /locus_tag="IW833_000005"
     CDS             5656..6489
                     /locus_tag="IW833_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1306158.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6572..7690
                     /locus_tag="IW833_000006"
     CDS             6572..7690
                     /locus_tag="IW833_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1306159.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7684..9582
                     /locus_tag="IW833_000007"
     CDS             7684..9582
                     /locus_tag="IW833_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ1306160.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9576..10799
                     /locus_tag="IW833_000008"
     CDS             9576..10799
                     /locus_tag="IW833_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ1306161.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10885..12819)
                     /locus_tag="IW833_000009"
     CDS             complement(10885..12819)
                     /locus_tag="IW833_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1306162.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13045..13701
                     /locus_tag="IW833_000010"
     CDS             13045..13701
                     /locus_tag="IW833_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1306163.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13775..14128)
                     /locus_tag="IW833_000011"
     CDS             complement(13775..14128)
                     /locus_tag="IW833_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1306164.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14320..15249
                     /locus_tag="IW833_000012"
     CDS             14320..15249
                     /locus_tag="IW833_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ1306165.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15260..16096
                     /locus_tag="IW833_000013"
     CDS             15260..16096
                     /locus_tag="IW833_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ1306166.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16344..17120
                     /locus_tag="IW833_000014"
     CDS             16344..17120
                     /locus_tag="IW833_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1306167.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17296..18039
                     /locus_tag="IW833_000015"
     CDS             17296..18039
                     /locus_tag="IW833_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1306168.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18032..19612
                     /locus_tag="IW833_000016"
     CDS             18032..19612
                     /locus_tag="IW833_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ1306169.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19863..20342)
                     /locus_tag="IW833_000017"
     CDS             complement(19863..20342)
                     /locus_tag="IW833_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ1306170.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20358..21110)
                     /locus_tag="IW833_000018"
     CDS             complement(20358..21110)
                     /locus_tag="IW833_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ1306171.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21227..21297
                     /locus_tag="IW833_000019"
     tRNA            21227..21297
                     /locus_tag="IW833_000019"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21259..21261,aa:Gly,seq:ccc)
     gene            21658..22383
                     /locus_tag="IW833_000020"
     CDS             21658..22383
                     /locus_tag="IW833_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ1306172.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22638..22904
                     /locus_tag="IW833_000021"
     CDS             22638..22904
                     /locus_tag="IW833_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ1306173.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22901..23332
                     /gene="ruvX"
                     /locus_tag="IW833_000022"
     CDS             22901..23332
                     /gene="ruvX"
                     /locus_tag="IW833_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ1306174.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23357..23662
                     /locus_tag="IW833_000023"
     CDS             23357..23662
                     /locus_tag="IW833_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ1306175.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            24231..25067
                     /locus_tag="IW833_000024"
     CDS             24231..25067
                     /locus_tag="IW833_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ1306176.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            25132..25563
                     /locus_tag="IW833_000025"
     CDS             25132..25563
                     /locus_tag="IW833_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1306177.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEA
                     KKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(25631..26953)
                     /locus_tag="IW833_000026"
     CDS             complement(25631..26953)
                     /locus_tag="IW833_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1306178.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(27107..28747)
                     /locus_tag="IW833_000027"
     CDS             complement(27107..28747)
                     /locus_tag="IW833_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ1306179.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            29077..29547
                     /locus_tag="IW833_000028"
     CDS             29077..29547
                     /locus_tag="IW833_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ1306180.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            30176..31279
                     /locus_tag="IW833_000029"
     CDS             30176..31279
                     /locus_tag="IW833_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1306181.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            31563..32243
                     /locus_tag="IW833_000030"
     CDS             31563..32243
                     /locus_tag="IW833_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1306182.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            32333..33367
                     /locus_tag="IW833_000031"
     CDS             32333..33367
                     /locus_tag="IW833_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1306183.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            33381..33716
                     /locus_tag="IW833_000032"
     CDS             33381..33716
                     /locus_tag="IW833_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306184.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            33700..36180
                     /locus_tag="IW833_000033"
     CDS             33700..36180
                     /locus_tag="IW833_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306185.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            36236..37795
                     /locus_tag="IW833_000034"
     CDS             36236..37795
                     /locus_tag="IW833_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1306186.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            37792..39156
                     /locus_tag="IW833_000035"
     CDS             37792..39156
                     /locus_tag="IW833_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1306187.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            39327..40397
                     /locus_tag="IW833_000036"
     CDS             39327..40397
                     /locus_tag="IW833_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1306188.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            40412..41080
                     /locus_tag="IW833_000037"
     CDS             40412..41080
                     /locus_tag="IW833_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1306189.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            41100..41678
                     /locus_tag="IW833_000038"
     CDS             41100..41678
                     /locus_tag="IW833_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1306190.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            42081..42992
                     /locus_tag="IW833_000039"
     CDS             42081..42992
                     /locus_tag="IW833_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1306191.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(43248..44165)
                     /locus_tag="IW833_000040"
     CDS             complement(43248..44165)
                     /locus_tag="IW833_000040"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ1306192.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            44367..44804
                     /gene="zapA"
                     /locus_tag="IW833_000041"
     CDS             44367..44804
                     /gene="zapA"
                     /locus_tag="IW833_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ1306193.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            44862..45410
                     /locus_tag="IW833_000042"
     CDS             44862..45410
                     /locus_tag="IW833_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ1306194.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            45525..47885
                     /locus_tag="IW833_000043"
     CDS             45525..47885
                     /locus_tag="IW833_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1306195.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            47972..48286
                     /gene="trxA"
                     /locus_tag="IW833_000044"
     CDS             47972..48286
                     /gene="trxA"
                     /locus_tag="IW833_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ1306196.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            48468..50246
                     /gene="uvrC"
                     /locus_tag="IW833_000045"
     CDS             48468..50246
                     /gene="uvrC"
                     /locus_tag="IW833_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ1306197.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            50391..51290
                     /locus_tag="IW833_000046"
     CDS             50391..51290
                     /locus_tag="IW833_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1306198.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            51283..52545
                     /locus_tag="IW833_000047"
     CDS             51283..52545
                     /locus_tag="IW833_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1306199.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            52708..54606
                     /locus_tag="IW833_000048"
     CDS             52708..54606
                     /locus_tag="IW833_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ1306200.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(54905..55624)
                     /locus_tag="IW833_000049"
     CDS             complement(54905..55624)
                     /locus_tag="IW833_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ1306201.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            55961..56293
                     /locus_tag="IW833_000050"
     CDS             55961..56293
                     /locus_tag="IW833_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306202.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            56280..58283
                     /locus_tag="IW833_000051"
     CDS             56280..58283
                     /locus_tag="IW833_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ1306203.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKHQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            58302..58772
                     /locus_tag="IW833_000052"
     CDS             58302..58772
                     /locus_tag="IW833_000052"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ1306204.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            58793..59377
                     /locus_tag="IW833_000053"
     CDS             58793..59377
                     /locus_tag="IW833_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ1306205.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            59392..60378
                     /locus_tag="IW833_000054"
     CDS             59392..60378
                     /locus_tag="IW833_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ1306206.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            60368..60679
                     /locus_tag="IW833_000055"
     CDS             60368..60679
                     /locus_tag="IW833_000055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ1306207.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            60701..62482
                     /locus_tag="IW833_000056"
     CDS             60701..62482
                     /locus_tag="IW833_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ1306208.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            62475..63851
                     /locus_tag="IW833_000057"
     CDS             62475..63851
                     /locus_tag="IW833_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ1306209.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            63858..64493
                     /locus_tag="IW833_000058"
     CDS             63858..64493
                     /locus_tag="IW833_000058"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ1306210.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            64523..65878
                     /locus_tag="IW833_000059"
     CDS             64523..65878
                     /locus_tag="IW833_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ1306211.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(66070..67464)
                     /locus_tag="IW833_000060"
     CDS             complement(66070..67464)
                     /locus_tag="IW833_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1306212.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFNSPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            67556..68527
                     /locus_tag="IW833_000061"
     CDS             67556..68527
                     /locus_tag="IW833_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ1306213.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            68524..69306
                     /locus_tag="IW833_000062"
     CDS             68524..69306
                     /locus_tag="IW833_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ1306214.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(69475..69846)
                     /locus_tag="IW833_000063"
     CDS             complement(69475..69846)
                     /locus_tag="IW833_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1306215.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            69980..70423
                     /locus_tag="IW833_000064"
     CDS             69980..70423
                     /locus_tag="IW833_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1306216.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(70466..71275)
                     /gene="yidA"
                     /locus_tag="IW833_000065"
     CDS             complement(70466..71275)
                     /gene="yidA"
                     /locus_tag="IW833_000065"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ1306217.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(71294..72661)
                     /locus_tag="IW833_000066"
     CDS             complement(71294..72661)
                     /locus_tag="IW833_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1306218.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(72645..73475)
                     /locus_tag="IW833_000067"
     CDS             complement(72645..73475)
                     /locus_tag="IW833_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ1306219.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            73638..74075
                     /locus_tag="IW833_000068"
     CDS             73638..74075
                     /locus_tag="IW833_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ1306220.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            74151..74771
                     /locus_tag="IW833_000069"
     CDS             74151..74771
                     /locus_tag="IW833_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ1306221.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            74960..75466
                     /locus_tag="IW833_000070"
     CDS             74960..75466
                     /locus_tag="IW833_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ1306222.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            75483..76382
                     /locus_tag="IW833_000071"
     CDS             75483..76382
                     /locus_tag="IW833_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ1306223.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            76469..77353
                     /locus_tag="IW833_000072"
     CDS             76469..77353
                     /locus_tag="IW833_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1306224.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(77545..77616)
                     /locus_tag="IW833_000073"
     tRNA            complement(77545..77616)
                     /locus_tag="IW833_000073"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(77581..77583),aa:Arg,seq:ccg)
     gene            77676..79010
                     /gene="rpoN"
                     /locus_tag="IW833_000074"
     CDS             77676..79010
                     /gene="rpoN"
                     /locus_tag="IW833_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ1306225.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            79213..80250
                     /locus_tag="IW833_000075"
     CDS             79213..80250
                     /locus_tag="IW833_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ1306226.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            80291..81292
                     /gene="gap"
                     /locus_tag="IW833_000076"
     CDS             80291..81292
                     /gene="gap"
                     /locus_tag="IW833_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1306227.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            81402..82592
                     /locus_tag="IW833_000077"
     CDS             81402..82592
                     /locus_tag="IW833_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ1306228.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            82696..83451
                     /locus_tag="IW833_000078"
     CDS             82696..83451
                     /locus_tag="IW833_000078"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ1306229.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            83567..84865
                     /gene="eno"
                     /locus_tag="IW833_000079"
     CDS             83567..84865
                     /gene="eno"
                     /locus_tag="IW833_000079"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ1306230.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            85265..86404
                     /locus_tag="IW833_000080"
     CDS             85265..86404
                     /locus_tag="IW833_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ1306231.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(86471..87046)
                     /locus_tag="IW833_000081"
     CDS             complement(86471..87046)
                     /locus_tag="IW833_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1306232.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(87051..88025)
                     /locus_tag="IW833_000082"
     CDS             complement(87051..88025)
                     /locus_tag="IW833_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ1306233.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(88049..88093)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            88308..89276
                     /locus_tag="IW833_000083"
     CDS             88308..89276
                     /locus_tag="IW833_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1306234.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            89389..89667
                     /locus_tag="IW833_000084"
     CDS             89389..89667
                     /locus_tag="IW833_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1306235.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            89785..90021
                     /gene="secG"
                     /locus_tag="IW833_000085"
     CDS             89785..90021
                     /gene="secG"
                     /locus_tag="IW833_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ1306236.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            90133..90879
                     /locus_tag="IW833_000086"
     CDS             90133..90879
                     /locus_tag="IW833_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1306237.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            90882..93239
                     /gene="rnr"
                     /locus_tag="IW833_000087"
     CDS             90882..93239
                     /gene="rnr"
                     /locus_tag="IW833_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ1306238.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            93261..93725
                     /gene="smpB"
                     /locus_tag="IW833_000088"
     CDS             93261..93725
                     /gene="smpB"
                     /locus_tag="IW833_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ1306239.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            94027..94746
                     /gene="atpB"
                     /locus_tag="IW833_000089"
     CDS             94027..94746
                     /gene="atpB"
                     /locus_tag="IW833_000089"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ1306240.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            94799..95014
                     /gene="atpE"
                     /locus_tag="IW833_000090"
     CDS             94799..95014
                     /gene="atpE"
                     /locus_tag="IW833_000090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ1306241.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            95105..95629
                     /gene="atpF"
                     /locus_tag="IW833_000091"
     CDS             95105..95629
                     /gene="atpF"
                     /locus_tag="IW833_000091"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ1306242.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            95616..96158
                     /locus_tag="IW833_000092"
     CDS             95616..96158
                     /locus_tag="IW833_000092"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ1306243.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            96181..97737
                     /locus_tag="IW833_000093"
     CDS             96181..97737
                     /locus_tag="IW833_000093"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ1306244.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            97751..98653
                     /locus_tag="IW833_000094"
     CDS             97751..98653
                     /locus_tag="IW833_000094"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ1306245.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            98676..100082
                     /gene="atpD"
                     /locus_tag="IW833_000095"
     CDS             98676..100082
                     /gene="atpD"
                     /locus_tag="IW833_000095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ1306246.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            100096..100518
                     /locus_tag="IW833_000096"
     CDS             100096..100518
                     /locus_tag="IW833_000096"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ1306247.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT        33229 a        17530 c        20936 g        29130 t
ORIGIN      
        1 aaataaatag gaacaaaaaa attaaattat ttaatacaca cattagggat cattcattga
       61 aggattagaa gacaacgagg atggcgaaaa atatgattac aatagaaaag tcgttctaaa
      121 ccaatcagtt cctagtatca cacaacgcaa gccagtcgat ttggtgagat tagaggagtg
      181 aaagcaaact ttattatata agaaaaaata ttcgaaaggg atgctagttt aggtactagt
      241 gctgatagat taatatgatg cctcgtaata ttaggcgtag ttactctaga gacatcacta
      301 tggactatga tttgtctcag agtcacaaca tgttttttta tgaaaagaaa cagctaaaat
      361 aattaaattg cgcaatgttt aaactttctt aaactataac ctaataaatg tgaagaaata
      421 atgcacaatt tcttcacatt tatcttctat tctatataca taccaaccaa caaccctata
      481 tactttttca tttttactcc tcaagtaaaa ataactccta gaccgcggct acccaccgcg
      541 gtcgtttttt tattcttcaa tcaaaatcgc tttgataccc aaacctgcca agattcgggc
      601 agaagtataa gtattcaatc ctcttctgaa agtaatgtaa acttttttat cttttgggat
      661 ttcattgatt cgatctcgta actcattcat tggaatattt tttgttgccg agatactgcc
      721 agaaggtgct ttattaggtt cacgtatatc taaaaagaac gcttctttca aatcgttttc
      781 gggtatatca cttgctttga tcgtttctac gatattcgtc atttgattga ttgcgacata
      841 accagctata ttcaacgggt cacgggtagt agaatacggt ggagagtaag gcaactccaa
      901 atcaggcaaa tcaaagactg tcaagtttcc ggtgattgct acagaaagtt ctcccatccg
      961 tttatcgaca cctttttctc caaatgcttg tccacctaaa atacgaccgc tatctgcatc
     1021 aaaaatcagc tttagattta atcttgtagc ttctggatag aaataggcat ggtcaaaggg
     1081 agtgataaaa acagttttat aatttgttat gcctgatgct tttaattcat gttctgtcat
     1141 tcccgcataa cttgctgtat gatcaaagat ttttgcgaca cctgcaccaa tatatccgcg
     1201 atagcgcata ggcgtaccat ttaaaatatc tgccagcata tgtccttgac gattggccgc
     1261 actggaaagc atactttgga tcggctgacc agtcactaca ctagtcgttt cgataatatc
     1321 gccaatcgca tagatgtccg gcagattggt ttgtaactgg tcatcaacaa tgatctgacc
     1381 tgtatctgat aattgtatgc cggttgcttt caccacttca ttcttcggag aaacaccaac
     1441 agcaaaaatt agcatatcag cagaaaggac ggagccatct gataatacta tttctcgacc
     1501 tttatcacgg atctcagtaa ctcttgtttc taaatgaacg gctaatcctt ctttcagcaa
     1561 tttgtcataa actaaattag caatttctgg atcataaggg aaagcgactt gtggcaattg
     1621 atcgattaaa gtcacgtcta agccgcgatg cttgaaattc tcagctaatt ccagacccat
     1681 aacacctgca ccaagaatga cgacatgttg tggatttttt tcttcaagga acgatttgat
     1741 tcgatccgca tccgtcacac tacggaggac aaagccgttt tctgcttcaa aaactccggg
     1801 aatatccgga taatctggtc tagcaccaga agagatgatc aacttatcgt aatctgtttt
     1861 tatttcttca tttgtggata ggtctttaac tgttaatcgc ttagtagaag gatcgattgc
     1921 tgttacttcg tgtttagtaa agacatcgat gttgtttttt gttttcaata tttctggtgt
     1981 acgttcgatc agcgagtcac ggtcagtgat cacacctcct aaataataag gcaaagcaca
     2041 acttgcataa gaaatatttt ctccgcgttc atagataatg atttcgtgcg cttcattcaa
     2101 tcgacggaaa cgagtggcaa atgaaggacc gccagctaca ccgccaatga caacaacttt
     2161 cataaaaaca tctccttttc tacgttgatc gtactatgtt atggtaaaaa acagcaaata
     2221 attgtgccgg aaaattattg tttattttga atagacagaa aatatagaga aataatttgt
     2281 ggataaaacc taaaaatttt tttctatttt aaggatagat aatactttga aggggataac
     2341 cctgtatatt ttttaaaagc tgaagaaaaa tgatggatcg aatgaaatcc taatatatct
     2401 gcaatttctg tataggttaa ttcattttct agcacgagac gttttgcttt ttccattttt
     2461 agttctttaa aatagctaat aacacttttt ccattcactt ttctaaaatg tttttttaag
     2521 ctggctggac tgataaaaaa aatttgggct aattgttcaa gcaaaaaatt ttctgaaaga
     2581 tgttcttcta aatatcgtat gattggatca actaattcat ctgaataaag aatatgcttt
     2641 tttttaggaa tgactttctc atttgaaggt attgtcaata aagcaagttc ctgttgataa
     2701 tgccttggta aggaagacaa ttgaattttt tgcagacaat aagtaactaa taaggaaagc
     2761 aaatgaatgc tcatctgttg gcatagtatg aaaaaagaat tgatttcctg atcataggaa
     2821 atagctggct tactatgttt tagataatca attttgaact gttcattttt cagaaaagta
     2881 atgatagatg ttatttcttg atctatagaa aaagattgtc ccgtaatatg tgctaataat
     2941 tgctcatcaa aataaaactg gataatgata aaagtttgtg tttccttttg agtagaaaag
     3001 atttggactt cttgctgttt atcaatgaat aaaagggaag gattatctag ttctatagat
     3061 ttatgtgcaa attcagcata taaattagcg ctttcattta ttagtagtcg aaaataggga
     3121 gcagcagata aatgaagatg ggtatctatg gaataggtta gtgttttttt gacaagattg
     3181 gaggattggt tgtttgttaa caaaagaacc actttccttt ctctttttat ataaaaagct
     3241 ttctatttat aattaatgac tattataaca aaaatgaaaa gccaaaacga caagtaaaat
     3301 atttttttat ataaatattt gtgttactaa ttccgttaat atacataagt gaaagcgttt
     3361 tatataagaa cttgaaaaga aagaagggat agacatgaaa aaaagataca aattggtgat
     3421 agctttgcta aacattaccc tattattcag cttaagtgca tgtggaaaaa ctaacgaaga
     3481 agtaacagca aacatcgcag atccaatgat aaaaaaagcc actacattag aagtatatgt
     3541 cgcaaacggc aaactgacaa actatgttga aaatgctgca aaaatctata accgcaaaac
     3601 tggagcaaat attgacttaa aaattgtgaa tgtagcttcg ggaacagcaa cagtacaaat
     3661 gatcacccca aaattggtaa cacacgaaga tatgccggat attatctcca tggcagacac
     3721 ttcagcagca ggtgtgctgg aaaaatttca agatgctttt tatagtgctg atgaatatgg
     3781 attttatgac aaatatggcg atcagtttta tgatcaaaaa ttgaatattt tgcgtcaaca
     3841 aacgagtaat gaaacgatta ttccttgggc tagtgatttt accccagcac tttgttatta
     3901 tcaaccaaaa aaatttgctg aaataggtgt agattttgaa gaaatcagtt cttgggaaga
     3961 atatatccag gtggcaaaag cattaaaaga aaaaactggt agttttggaa ttgccttgcc
     4021 agaatctggg gaccaagaat tgtttatcaa catgctagcc cagcagaatc aatctttgct
     4081 tgatgaagaa ggaaatatcc gtctgaatac taaagaagcg aaacatgctg caaagttaat
     4141 caaagaaatg atcgactcag atattgtaca tttctacggt gcacaagatg ctgagaaagc
     4201 atttcaagaa tctgcaatgt ttgttgctgg cggctggtat gctacgaata tgtctttgaa
     4261 ttttcctgat gcatctgaca aatggcgcat ggctccactg attccttttt caaaagaaaa
     4321 tccaggaaaa ggtgcagtat caggaggatc aagtttttat gtccctaaag atgccaaaaa
     4381 tccacaagtg gcacaacaat ttcttacatt tatgctgaca gatgaagaat gcttagccaa
     4441 cgcactggag ctgggcgtgg ctacatctaa ctcattggcc tatcaaacag aagcagctga
     4501 gaaaaagttt gactactaag gaggacaaaa atactatgaa ctattgaata aaatcaatga
     4561 aaatacatca aatactgttt taccagccac ttattcagat gctacgtctt atttgactac
     4621 cgctgcatat aactattggc aaacaggtga ttttgaaaat tcatatctaa aagaagcaga
     4681 aaattttgct cataagtacg gcgtaaaatt aactggtgat taaaaaatat taggaggact
     4741 taacgatgat gcaatcttcg ttaccaagca agacaataaa gaacagcaag aaaaaagcaa
     4801 cagatacgtc tcataaaaaa agtgcttatg gattggtcac accttttgtt attttcttta
     4861 ttgtgacgat tttgattccc atcattatct cgtttgcttt tagttttatg aaaattggct
     4921 actcatttga atttgttgga tttgacaatt atctagctat cttccgagat ccattgttta
     4981 ttaaatctta cacgaatgtt ttgattatca tggcaggatc aattccaata acgatgctat
     5041 tagctattta ctttgctgtt ttattgaatt cagcacagtt aaaaggaaaa ggatttttta
     5101 gaactgtcta ttatattcca accattacat caacggtggc tgtagcttcc gtattcatga
     5161 cttttttcaa tccgactggg ttattcaatg gatttttgaa tgcgctagga attaacggtg
     5221 tacagtggtt aacaaatcct ttttggatac gaatatcaat gatcatcacg atgatttggc
     5281 ttagtgtagg atataacact attttatttt tagctggtct tcaagggatt tctaaagaga
     5341 tatacgaaag tgctgaaata gatggagcat cgaaatttcg gcaattcttt ttattaacga
     5401 tccccatgct aaagccaatc attttaatgg ccacagtttt agccactatc aatggcttgg
     5461 gtaatttcaa tatcccaaat atattctttg ggacttccaa tggaccagaa aattcagctt
     5521 tggtcgtagg agtcaattta tataaaacaa gttttgaaat ggttgatttt ggaaaagcct
     5581 ctgctattgc ctggacgatg gtgtttgtat ctgttatatt atcggctatt caatttaaat
     5641 tcggaggtga taaaaatgac taaacaaaag atacaaagca gaatttttac gtacgtcatt
     5701 attgtgtttg gtgccttaat atttttgatg ccatatttat atatgattgt agcttcaacg
     5761 caaagtaatc aagaaatttt aagtggcgga atcaatttca agtttgggga tatgctatta
     5821 aaaaactggc aagccatcat ggcgagatat aattatccct tgattatttg gaattcgatt
     5881 gttattgctg taatcagtac agttttagct acagccagtt ctacaatggc gggttatgca
     5941 ttagcaaagt atcgttttat ggggaataaa ctgttgttca atacagttat gctatctagg
     6001 atggtgcctt tttttaccac attgattcct ttattttaca ttatgagtcg tttaggatta
     6061 tcgaatactt atgcaggtgt gatcatccct tcgattgcca gtacaacttc agtgtttatg
     6121 atgcgacaat atagcagtca atttcctaca gcactgatgg aggctgccag aatcgatggt
     6181 gccaatgagt gggtgatttt tacgaaaatc gctgtaccga ttttatcacc tacgattatt
     6241 actagtggtt tacttatctt tatgggaagt tggaatcaat atctatttcc attagtcatg
     6301 ctttcagata aagataaata tacggttccc ttaattatca aaaatatgac ggtttctagt
     6361 acaggtgagc caattaatta tggcgcaatt atgctaattt tagcaacttc agtgatccca
     6421 atggtcttga tttatgtgtg ggcgcagtct aaatttaagg agaatgacat tggagctgca
     6481 aataagtaaa aaaaataacg aatgttaacc atctagttcg ttctaggagc aactccacat
     6541 tcataaaaat taaaagaatg gcgggatatc catgaatgaa cagacgaagg aatggtcaag
     6601 aaaaattttg aatgtagctt ctgaaaagat agatgcggaa ttagacagaa tggggaataa
     6661 aattccatat atttcttaca acggaaaata tacagacgta ataaaggaca gaggaatcga
     6721 ctggtggacc aatggttttt ggagtggtat tttatggcag ctctttcaca agacaaaacg
     6781 agaaaagttc aaagaggcag caatacaaca agaacaatta ttagaatctg ccctgtacca
     6841 tttttcagac gttcatcatg atgtaggttt tttattctta ccatcggcta ttgcacatta
     6901 tcaacagtct caaaacaaac atagtcgaca actagggtta catgcagcta cggtacttgc
     6961 tggacgctat aatatttcag ggaactattt agtagcatgg aatgaccata aacctggttg
     7021 gatgattatt gatagtatga tgaatttacc attactgtat tgggcttcga gggaaactgg
     7081 tgatccgagg ttctctaaaa ttgctcggca gcatgcagat acaacagcta agtatctagt
     7141 gaggaaagat ggatcaaccg ggcatatcgc agaatttgat gcagaatcag ggaaatatat
     7201 tcggttactt ggcgggcaag gatacaatga gaattcttgt tggagcagag gaaatgcatg
     7261 ggctttatat ggattcgcgt taagttatcg ttacacaaaa aatccgtact atctagctat
     7321 agcagaaaag atagcagtaa attttattca gaaaatcgaa aaaactggtt atattcctct
     7381 cattgatttt gctgtaaaag attcgaatat tgataccagt gctggagtat gcgctgcatg
     7441 cggtcttctg gaattagcag gatatgtttc ctctgtacga tcaaaaatgt atcatgaagc
     7501 tgctatcaat atactccaaa agacagaaaa gcactatgca aattggaata tggatgaaga
     7561 tgggatcatt ggaggcggag cagaagcata ccatagagaa ctgacttata atgtgccact
     7621 gatctattct gattattttt ttattgaagg cttacttcga ttagaacagt cggaattagc
     7681 tatttggtag taaaaagagg agaaaataca atgaaacatc cagtaccact attaaggacc
     7741 agacaggatt actttgactt atttaaaaag ataaatcaac cactccagcc gttttatcga
     7801 gcaagtggcg cacaaattga ttatatacat caaggggtag gatatggaaa taaaattgca
     7861 ggaatggaag gattctctcg ggttttatgg ggagcggggc ccgcaattga ccaattagat
     7921 gaacgatggt tgaaattaat tctatcagga ataaaagctg gaacagatcc ttcccatccc
     7981 gattattggg gagaaataca tgaaagagac cagcgtatgg tagaaatgcc cgcaatgtta
     8041 ttagcgttat atcatcatga acaattatta tggaaaaaat tagatacagg agaaaaaagc
     8101 cagattattc attggttctc gcaaattttt gaatatgaat gtgcagatgg taattggcaa
     8161 ttttttaaga taattgtcgg aacaattatc caaaaatttg gttatccagt taaagaatca
     8221 gcactgaaag aagcatttga taatatagaa aattgttatc ttcaaaatgg ctggtatcga
     8281 gacagttcaa gaggtcgcca agattattat aatccttttg catttcatta ttatggtttg
     8341 atttatagcg tactagaacc agaaaagccc attagccaga tatataagaa ccgagctatt
     8401 gatttttcta gagactatat tcattttttt gcggaagatg gtgcaaatgt gccttttggt
     8461 cgaagcatga tttatcgttt tgcagtttca agcttttggt cagctatgtt gtgggccgat
     8521 cttttgcctt ttaaattagg tgaaatcaag gggttagtca atcggaatat aagatggtgg
     8581 atgcaaaaag aaatatttga tgaaaaaggt atcctgaata ttggttatac ctaccctcaa
     8641 ttgacattga ctgaacccta taatagtacg ttatctccat tatggctgaa taagattttt
     8701 ttattgttgg cgctacctga agaccatgag tattggacgg ctaaagaaat gcctatgcct
     8761 attcgtgaag gaactaaact tctttcagaa gccaatttgc tcatacaaca tgataatgga
     8821 catacatttt ttttgaatgc gggacaatta gggccaaatt tccatacatt gaccaatgaa
     8881 aaatatttaa aatttgctta ttctagtcaa tttggttttt cgataccgcg agcgaatcaa
     8941 ttgaaagcag aaatggcaat ggattcaatg ctagggattc agcgagcgga cacatatatc
     9001 acgaccagtt ttaagaatga gagcatcaaa acctatggac agttcatcgt tcgaaataat
     9061 gtacgagaca ttgtatgtaa gaaagaggta cttgcctcta catgggaacc tactttacag
     9121 gcaaaaatca gaacttggct tattcctttt gaaggctggc agattcgcgt acacaaagta
     9181 gaattagata cagaatatgt tctatatgag acagggtttg ctattgaatg ttctcctgag
     9241 aaagaaggaa taatcataga agaagacaag gaaaactata gggtgagtga agctggcttt
     9301 tcaggtatta tctgtttaag tgacgatact attaaacgaa aatccacagc tattatgggt
     9361 ttgcccaata caaacttgat gacatgggaa aacactttta ttcctggatt agagggtaca
     9421 tttggaaaag gaacccattg gctcggaaca ggagtggttg ctcatcaaga tcgggattat
     9481 agtttagaag tatggaacaa tcgtcccaaa attgattttg atgggacaac cgggctaact
     9541 atccaaaata aaaacaataa aaaaagacta aaattatgct aaaggctggt ggggaaatgc
     9601 ttgataaagt gatgtttgaa gagataatac atccagaaaa atggataaat gaatgttata
     9661 aagaattaat ggaaaaagaa tggaataatg agatcgagcg ttgtcgcaga cacacggaga
     9721 ggttaatcaa acgtatcagt aacagtcagt atcaattccc aagcgagtgt cctataaaag
     9781 gacgttatcc attaagagac aatattggtt ggacagaagg attttggaca ggaattaatt
     9841 ggttgttatt tgagaagacg ggtgatcctt tatttcaaca aacagctgaa aaagcagata
     9901 tagattttat ccacaggatt gactgtcaaa ttatcactaa tcatcacgat cttggttttt
     9961 tgtattcttt atcaaccgta gcagattata aaataacggg aaatcgaaaa atgaaggaag
    10021 cttcaattga tgctgcagaa acattacttc agcgtttttg ggagaaggca ggagttatac
    10081 aagcttgggg agatcctagt gatccttcac aacaaggtag aatgatcatg gattgttgca
    10141 tgaatcttcc attattgtat tgggcgagtg aagaaactgg aaatcaaaaa tataaaaaag
    10201 ctgctcaggc tcatattcaa aatgcagcag attatttagt aagagaagat ggttcaactt
    10261 atcatacatt ttatatggat gtcaaagatg gaaaaccaag atatggaagt acgcatcaag
    10321 gttatagtga taattcctgt tgggctagag gccaagcatg ggggatttac ggttttgcat
    10381 taagttatcg ttatttgaaa gaacaaaaat attttgattt aagcaaaata ttagccaatt
    10441 attttatgaa tcggttgcca gaagatacca tttgttattg ggatttgatc tttgatgatt
    10501 ctagcagaga agaaaaagat acatctagta tggtgatcat ggtttgtgga ttattagaat
    10561 taatagaatc aggtctatta gacaaaacag aaaagaactt ataccagtct gctgtttgga
    10621 aaattatgcg acaattgaaa gaaagatatg ctacaacgga agaagaagat ggctttttaa
    10681 cacagtctgt ttataataag cccaaaaata atggggtaaa tacaccttgt atttggggtg
    10741 attactatta tttagaagct ttagtgagaa tgactagtaa ttggtcttct tattggtgaa
    10801 aaaggtaaat atggaatttt tgtttgataa aataaaaaaa gaaaaaccaa aacagcatga
    10861 atggtttctg ttttggtttt ttcgttaatt ttcatattct tctaattcca agcttagttc
    10921 ttcccattcc gtcaattttg tttcttgatc ttgtctagca gcttctaaat cttggttcag
    10981 ctcattgagt tttacgtgat cttccaggtt ttctggcgcg accatttctt gttccaactg
    11041 atcgatccat tcatcgatcc gcgccatttc ttcttcaacc tgagtaattt ttcgttgaag
    11101 gctacggata agcttttgct gttctttgct ttggtaataa tcagatttgt tcgtgggagc
    11161 aggagcagat tctggctcat cttgtgccaa caattcggcg atttcttctt cttcctgctt
    11221 tttctctaaa taataatcat agtctcctag atagactttg ctgccatttt cagagagttc
    11281 gacgactttt gtcgcgatcc gattgatgaa atatcggtca tgggaaacaa aaagcagtgt
    11341 gccgtcataa tcgatcaatg cattttctaa tacttctttg ttgtcaatat ctaaatggtt
    11401 tgttggttca tccaaaatca aaaaattttc tttgttcatc gctaacttgg ctaaagcaac
    11461 ccgtgctttt tcaccgccac ttagcagagg gattggtttt tctacatctt cgccagaaaa
    11521 taagaaagca cctaaagcac ttcggatctc tttttctgga gtggtaggat gctcatccca
    11581 caattctgct aggatcgtct tgtttgtatg cagatcagct tgaccttgat cgtaatagcc
    11641 gatggtgaca tttgtcccta gcgtttcttt tccacggata aaagggatct ggtgaatgat
    11701 tgatttcagc aacgtcgatt tcccgatacc attcggacct actagagcaa tcgcttcttg
    11761 acgacggata tctagattga ttggttcaga taaggtgaca ttatcatatc cgatagcggc
    11821 atcttcgatt tgcaagacta catttccaga aggcttttct atactgaata gaaagtttgc
    11881 ttgtttttca tccccttgag gacgatccag ccggtccatt ttttctaatg tctttctgcg
    11941 gctttgcgct cgttttgtgg tagaggcgcg aacaaggttc cgagcaacga agtcttctaa
    12001 tttatggatt tctgtttgct gcttttcata agccttccat tcacttgcta actgttctgc
    12061 tttcaaatct agatatttcg aatagttacc tttgtaatgg cgcatttttc ttctgcttaa
    12121 ttcatagact tcattgacta ctttatctaa aaaataacgg tcatgggaaa caatcaataa
    12181 tgcaccagta tagctttgca gataattctc aagccaggaa agtgtctcga tatctaaatg
    12241 gttcgttggt tcatctagaa tcaaaatgtc agggtgttgc aaaagcatgc gagctaaagc
    12301 taaacgtgtg cgctgtccgc ctgataaagt atcgattttc tgatcataaa atgaagaatc
    12361 gaatttaaac ccatgcaata cggaacgaat ttcattttca taaccatagc cgtttcgatc
    12421 tgaaaaatcg tgttgcagcc ggtcgtattc ttttaaataa gaactataag caggtgtgtc
    12481 tggcgtacct tcgctaatga aaatctctag ctcgcgcatc cgctcttcca tttgtctaac
    12541 ttgttcaaaa gctttcagca tctcgttcca aactgtttca ttggaatcaa gtcctgtgtc
    12601 ctgagcgagg tatccgagag tcgctgtttt atttttagcg atctttcctt catcaggtgc
    12661 ttcaattcca gcaatgatct ttaataaagt cgattttcca gcgccatttc tgccaactaa
    12721 agcgacacga gcgcctgtac tgatttctaa atgtatgttt tcaaataaga cttctgcacc
    12781 aaaatggcga gcaatctgat tggcttgtaa taatatcatg gaatcctctt ttctactttg
    12841 tgaattctcc tcaaataaaa aactattttt caattagtag tctaccataa attccattat
    12901 actgcgagat ttgcagaaat gataaataaa tgaataaaga ataaaatttc acaatatctg
    12961 agcaatggaa ttgatttttt cctataaata ctgctatgat ggagaaatca aagatgtaat
    13021 tagacaatac gtggaggtca tttcgtgaaa gatcatatta ttccaaaggc gacagctaaa
    13081 agactgcctt tgtattatcg ctatttaagg attttaaata atgctggaaa gaaaaaagtg
    13141 tcttcaactg aattaagtga agccgttcaa gtagatagtg caacgatccg tcgagatttt
    13201 tcttattttg gcgaattagg aaaacgtggg tatggttatg atgtagaaga tcttatgcac
    13261 ttttttgcaa agacattaaa cgacgatcaa ttgacaaatg ttgcgctgat cggtgtaggg
    13321 aacttgggaa gtgctttact aaaatacaaa ttccatcaaa gtaatagcat acgtatcagt
    13381 tgtgcttttg atgtgaatga ggaaatcgtt gggcgcatcg tagacggtat ccccgtttat
    13441 ccaatggaag atatgtcaga acaaatcaag atccaacaaa tcgaagtagc aattttaacg
    13501 atacccgctg aaagagcaca agaagtagtc aatcagttaa cggaagcagg aatcaaagga
    13561 atcttgaact ttaccgctgc tcgattgaat gctccgtcag atgtattgat ccaaaatgtt
    13621 gatttgacga atgaattgca aaccttgatc tatttcttgc attctgacgc aaatgcgcat
    13681 tccgaaaata cagcagaata aaaataaaaa gcgattgaac aaatgaaggc aaaactttca
    13741 cttgttcaat cgcttttttc tgtttggaca acgattattt tgttcggaag atttcttcat
    13801 aagaaaggga atcatcttct gctgtcagtt catctagtag ataacggatt tttttaaagt
    13861 cgatgaaccg tgcttgccga aagacttctt ttccttgatg aaaaatcagg acaactggcg
    13921 cagtcagcac ttcaaattta ctagctactt ctggtgtttc aaatgcatcc aaatgaagtg
    13981 ccggaatatc ataatgtgtg agaagttcct ctagtcgtgg acgaacagcg tgacaaacac
    14041 tgcaattagg catcgaaaga taaatgacgg caacggatga ctgttgcagt atgtcttgaa
    14101 cttcagtcaa gttagtggtt tgtttcattt ttattctccc ttttatgata gaagtgatat
    14161 gtatgagacc ttaatttaat gggaataaca gaaaaagtca attgaagtgc tttggtaaaa
    14221 aataagagtt gtgtaaaaat ttacagagca acttgttttt tttgaataaa agcgtatatt
    14281 tgcaaagcga atgaactaga aataaaaagg aggaaactca tgttttcttt tattccaaat
    14341 tcggttttgc agatgagtac gattttctta tcgatcgtta ttgaagcatt acctttcgta
    14401 ttattaggct gtcttatctc aggcgcctta caagtatttt tgaccccaga acgtgtgaag
    14461 agatggcttc ctgaaaatcg tatcttctct atttttactg gttcgatctt aggatttttc
    14521 tttccttcat gtgaatgcgg catcgtaccg atcgttcatc aatttgttaa aaaaggcgta
    14581 ccagttcaca cagcgtttgc ttttatgctg actgctccga tcatcaatcc aatcgtgatt
    14641 ttttccactt ttatcgcatt tggcaattct tggcagatgg caagctggcg ggtgataggc
    14701 agcttttttg ttgcccttgt gattggtgtc tggcttgcct attttcaaaa agacgctgta
    14761 ttgaaagaaa aaatcacgtc taaagtagca gaaaatcacc atcatcatgt ccatgaagaa
    14821 gaacaaagtg agaagaatga accaaaaaat cagctgatcc atcaaactgg gcatgtgttg
    14881 acacattcaa tcgacgaatt ttttgataca ggtcgttatt tgatcattgg cggattaatc
    14941 gcagctgcga tgcagactta tttaccaact agaattatgc taacgctcgg ctcaacaaag
    15001 attttagcca ttatcatcat gctgttattg gcctttacga tgtctctttg ctcagaagca
    15061 gatgcgttta taggatcttc tttgttaagt ctgttcggca cagcacctgt agctgccttt
    15121 ttggtctttg gcccaatggt cgatatcaaa aatctactga tgatgaaacg atattttaca
    15181 ggacgcttta ttttatcgct gatcggactg attgccctta gcgttattgg attcacatta
    15241 tttatttagg agggaaaata tgctgcggtt tttgatttta tctgggtatt tcgaattgat
    15301 gatgtacttg caagtttctg gcaaactcga tcaatatatc aatgtccatt atcgctactt
    15361 agctattctt tcaatgattt tatctgctct tttggcttta gttcagctgt atttgtgggt
    15421 caaaaacgga aaagcagaag aaaaggcaca cgagcacgat catgaccatg ggttgtcaaa
    15481 accttatcaa cgtggaatcg cttatgtact gctagctttg ccagtaatcg tggggacgtt
    15541 gtttccaaca gttagtctcg acactacgat cgtagaagcc aaaggattta atttcccagt
    15601 aagtaaagaa tcagtagggg atccggagat gcagacacaa tacttaaagc cggataccag
    15661 tatttatttc aacaaatcag actacttaga ccaaatgaat gagttgaaag aaaagtatgg
    15721 ggataagcaa actatcaaga tcacagatga aaactattta gaagttatgg agctaattta
    15781 taattaccct agcgaattca ttggcaaaaa aatcagctat gaaggatttg tttaccaaac
    15841 accgaacgat agcaatacag atatcttctt gttccgattc ggcattatcc attgtgtggc
    15901 agattcagga gtctttggtt tgttgacgca tatgccggaa ggagtaacgg ttaaaaatga
    15961 tgaatggtat aaaatagaag gaaccatcca atcagactat tatcaaccat tcaagcgtga
    16021 aatcccatct gtagttgtta caaacgctga gaaagtctct gctccgaaaa atcagtatgt
    16081 gtatcgttct ttttaaaaga acaaaataaa taaacatagc tcagacagct gacacgcaag
    16141 gacgtcggtt gtctggcttt ttttgcatga tgtattcatt tgtttgatat ttattttaga
    16201 agcagcatcc tgaaagtatt tgatatcggt ttcaaaggtg ttggaaaaga ttgtgaagag
    16261 aattacaatt tattcctaac atgtatgttg aaaagttgta tgaaatgatt ttgatagtaa
    16321 aatatcgagt ggaggtactt tttatggtaa cgcgtatatt tattttggat gatagtttgg
    16381 tttttgaaaa aatgctagta gaaattttgg aagaatccag aattttatta agtccttgga
    16441 aatttgagat cgttcaggga ttgaacgtta tacaatttgt tgaattcatt aaaaataatg
    16501 aaatctattc ctcagatatt tttttcctag acatcgattt gaacgcctat tatagcggta
    16561 tcgaactagg cgaaatgctt agagaaaaga atgaaaagtg taaaattgtt tatctaacgt
    16621 ctcatccgaa taaagcaata actgttatta atcgaaagat tacgcctagt ggttatttga
    16681 taaaagaatt cacttatgaa aaaaataaaa aaagcatcca aagttactta ttgaaagaaa
    16741 aagaaaacta tcctgttcaa catgctacga aaaaaaggtg gacaactgtc cgttatggta
    16801 atagcgatca ctatatccat tacgatgaaa tcctttatct aatgagcatg ccaggttata
    16861 aaaataaaat aggcctagta ttgaaagatg aagagattct cgtaaacggc acaattaaaa
    16921 aatataaaaa atcactcgaa tatgagtatt tatgtaaaga tttaaaagct tatatcatta
    16981 atactgaaaa cattacatct attagcagag taaacgaaat agtaacattt gcaaatggag
    17041 aacaactaga gatgggaaag agaagtattg ataaactaaa gaattttatg caagaaaaga
    17101 tagaaaatga atcattttaa agaaaacaaa gactcatatc ttacctgtta gaagagtgtt
    17161 ttcctgtaga agagaacaac ccaagtagca gcctagtcgt ttctatcctt ttttgttatg
    17221 cactcttttt gacatttact ccaccaataa cgaagggtac aataaaaagt aataacaaac
    17281 aaaaggagta cagaaatgat tgaattcatc aatgtaagta aaagttacgg gaaaaaacaa
    17341 gcattaaaag acttgtctct ctcgataaga caaggggaaa tttttggatt tttaggacat
    17401 aatggtgccg gaaaatcaac tacgattaaa agtttagtta gtattattga accatcatcg
    17461 ggaacgattt tagtagatgg aatgaaattg acggaaaatc gattgagtat caaacaaaaa
    17521 atcggttatg tgcctgattc cccagatatt tttttacaat tgacggctgg agaatattgg
    17581 gacttgattt cagctgccta tgaattagac acacaaaaaa aagaaaagcg tttagcagaa
    17641 ttgacagcgc tttttgatat gtatagtcat caaaatgaaa caatcgcaag tttttcacat
    17701 ggaatgcgtc aaaaaacgat tttgatcggc acgttgctgc cagatccaga tatttgggta
    17761 ttggatgaac ctttgcaagg acttgatcct caagcagcat ttgatttgaa agaaatgatg
    17821 aaggctcatg ccgcaaaagg aaaaacagtc attttttcta cacatgtatt ggataccgca
    17881 caacagttat gcgataaact ggctattttg aagaaaggtg aattgattta tcagggggca
    17941 gttagtgaat tattgaatgg ctcacctgat gaaacgctag aaaagattta tttgaaaatg
    18001 gctggaaggc aagcgacagg agatggaccg tatgtataag cgacaattat tagaattaat
    18061 tcgagttaat cttttatatg ttaatccaca gctcacgaat aaagcaagag agaaaggaaa
    18121 aaagggaaag cgattaacta tctatctttt gtctcaaaat cttttttcag ggctgatttt
    18181 tttactgatt tacggatttg ctatgtttgc tatgaacttt gttaaattgc caggtttctt
    18241 tacttattac gtggcacttt ttggtatgtt aggtttaact caaagtatat cagtcattta
    18301 taatatcttt tttgaaggaa atgacctgca agtatttttg cctctgcctt ttggacaaga
    18361 acaggttttt tcagcaaaga ttttggtagt tgctttgacc atcattcctt ttgtttttcc
    18421 gcttctagtc atgtttattt taaccggatg gcgagctgat ctagggattt tgcttactat
    18481 cattatctca atccttttgt tttctctatt tttgattatt cttttttgtc tggctagttt
    18541 gattgttttt ggtttggctc gaacagcagt gtttaaaaaa catagaaaaa cagtttctag
    18601 tatcttgctt ggcagctcga tgctgatcgc atttgctgga atcatgtgga tgaatcatca
    18661 aacggggact attgaaaatg agttaacaga tcggcatccg atttcgatat tattgccatt
    18721 ctatgatgct gccagtcagc cactttctca acaaggttta ttgggttggg gaatactagc
    18781 tatcacggct ctgggatttt tccttgctgt aaaacagctg atagtaccaa agcttacaga
    18841 gcaactgact gattcaaaca gtgagttcaa aataaaaaga acccataaaa agaaccaaaa
    18901 tgtgcatcag cttttattta gttacaatgc tcaattagta aaagaaccta atttgattat
    18961 gcaagttctt tcttcttcct tgttaactcc aatcattttt attcttgctt ttgccctagg
    19021 aggcgaaata aaattaactg atttagataa taagttaatc ggtgttgttt tcctggtagg
    19081 gatcgcattg gctttcttaa cagttaatca gacttctttt atcagcaatc ttatttcttt
    19141 ggatcaagaa aattttctgt ttatccgttc tttgccaatc tctatgaacc aatatttaaa
    19201 agaaaagttc agattcggat ggatattgca gagtttttta actggtggga tcgcattatt
    19261 aagcggactg tcttttcagc tgcctttgtt ttttatccct agcttgctat ttggttcttt
    19321 attcggctgc tacttattgt cactacgtta tttcgctaga gattatcggt tgctgctgct
    19381 gaactggacc aatattaacc aattatttaa cagaggttca ggaagtttgg gtttagtcgc
    19441 aactatgatg ggttcattga tcatcagtat tattcttatc gtcttatacg gaatgttggc
    19501 tttgttttat ccattttggt tagtgaatat tcctgtgatc ggtttagttc taatactttc
    19561 cgctctatgg atcgtttatt atcaacgaac gttttggaaa aaatttgaat aaagaagagg
    19621 ggccgtgaca aaaatcatgt cgcagcccct tttttacaga tagagggtgt tcaaaagtca
    19681 gtttctcagt attaagtcaa ttttatgtaa aaatgtgaga agctattttt acaaaacttt
    19741 ttttaatact cataattact tcttattgct cctgcggtta cttgtcgcaa tgtttgtgag
    19801 atattttgtc atcgctcaag gctgaacgtc tctttcacaa ccttttcttt gttacacttc
    19861 gtttatttaa aaagccgttt ttttcgtttc atcatgattg cgtcacgctt tactttgaaa
    19921 ttacggatgt tatggttaaa gacttctgat aatacaagac ctaaagcgat tgctccagca
    19981 atcataattg cttggaccgc ataatgagcc gcttctcgat aggattcagt aacgagattt
    20041 cgaaccgctt gataagctaa accaccaggg actagaggaa ccatcccggg aatattgaaa
    20101 atcgttacgg gcattttcaa acgtttagag aaaagatcgc tgatgaatgc tactcctaaa
    20161 gaaccaagca atgaaccaaa agcagcagat ccgcctattt gaacacagat ccagtagatc
    20221 atccagccaa aagcaccaga aagaccacaa caaatcaatg aacgcctagg tacatttgta
    20281 ataacggcaa atgccgcagt cgctaaaaaa ctaaaagaaa actgaataaa tatcgtcccc
    20341 atacttgtgt tcctttccta ataaaaaagc tggaaaataa aagcaatcgc aaaaccgatc
    20401 atcgtagcag tcatcatcgc ttctgttccg cgagaaactc ctgagacata atgtccagct
    20461 agcaaatcac ggatagcgtt cgtaatttga actccaggaa ctaaaggcat cacacatccg
    20521 ataatgatta gatcctgatt gcttcctaag ccaaaacgta cgctgtagat agcggctatg
    20581 ccaatcaata aagaagataa aaattccgat aaaaatttga ttctaaaaaa tttcaaacta
    20641 tatagataag ctgcgtatcc caatcctcca atgactaaag tcacaggtaa atcggtcatc
    20701 tttccgccaa ataacaccat aattgttcca ctgatcactg ccgcgctaac gatttgcaac
    20761 cagacaggaa agaaagaacg gtctcggtcg attgcttgca gttcgcagta aagttcttct
    20821 aatgttagct ctgaagcaac atatttacgt gaaagatcat ttacttttac tactttttct
    20881 aaattaatcg aacggttggt aatttgttcc attcggatgg tcgaggtacg atcaagacca
    20941 ataaagagac cggtttgagt gacatagctg accaaacgga aatttccaga agctaaagca
    21001 attcgactca tcgtatcttc tacccgatac atctccgcat tgctttccat catgattttc
    21061 ccagcgagta aacaagtgtc cagcaacaga tctaattctt ttcccgtcat agcatcttct
    21121 ccaataattg attgattagt tttattttac tacaaaacat accagaagcg taaagaataa
    21181 aaaaacttga ggaaggaata aaagaggcgt atactgtttt tgtattgcgg gtgtagttta
    21241 gtggtaaaat cccagcttcc caagctgatg tcgcgggttc gattcccgtc acccgcttaa
    21301 aataggttgt gaaaaagctg ctctgcaaca agtaataaga acagccaagc aaaaacaact
    21361 tcttatgttt ttaacttgga tggacttatt accgcagatg caagcttttg cgcaccgttt
    21421 accaagcggt tgtgaaatca gtgtattgtc ttgagtatga agaaaatttt tgaataataa
    21481 taaatacctt acagatagag tggtgactga ttagagacga ggaatccgtc tcttttttgt
    21541 atttttaagc catcttaaaa caggttttat tttttgctgt ttctatttct ttctagtatt
    21601 tatttatagg ggaacatatg ttaagattta gggagctgtt agtaaaggag taaagaaatg
    21661 agcaaaaaaa caaccaaggc gatcgtgggg atcgtcgctg ctgtgattgc aggggcattc
    21721 ggtgtttcca ccacacaaaa agattcagat atctttcagc agattacaac tatttttgat
    21781 tccacaccac aagcttctca agtagcggat gtgccagaat atgatggaaa acatcaagaa
    21841 atagaaatca atgggaatca gccggaattt acaaaagagg atcttagcct ttccaaagga
    21901 acatgggaaa gctattcaaa tattgataaa ttgaacagag taggacaagc caatgctatg
    21961 cttggaaaag atttgttccc tactgaaaag agagagcctt tgtatattga cccgacaggt
    22021 tggaaacaaa agaaattaag tgacgggcaa tggttatata atcgcagcca tttaattggt
    22081 ttccagctga ctggtcaaaa taacaacatt aaaaacttga tgacagggac acgttcgttg
    22141 aatgctccat acatgcttgc tcacgaaaat gatattgcgg catatatcaa agaaacaaat
    22201 catcatgtac gctaccgtgt cactcctcat tttgagggga atgaactggt agccagggga
    22261 gtgcaattgg aagcagaaag cattgaagac aaaaaaattg aattcaatgt ctttatctac
    22321 aatgttcaag atggctacac gatcaattat gagacaggac aagcaacaaa aacaaaaaat
    22381 tagtagcagc tattcgaatt ttttatgaat attatgaaaa aattgtgtaa aaacatacat
    22441 tcaaagaaat atagcgtata ctttatgaaa agagtacttt tgataaagct acagaaaaaa
    22501 ggaaaaaaac atacaaatag tgtttcttaa tttattcgga agtttggaat atttgtttgt
    22561 agttttccct ttcttcgagt aaaataaggt tgtagaaggt aagctggaat taccttgcgg
    22621 gaaagaggtg tagaatcatg ggttttacag atgaaaccgt acgttttggt tttgacgata
    22681 gtcgcaaaaa agaagtgagc gaaacattag cgattgttta tcgggctttg gaagaaaaag
    22741 gctacaatcc aatcaatcag attgtcggct acttgctttc tggagacccg gcctacattc
    22801 ctcgttatcg tgatgctcga aatttgattc gccgtcacga acgcgatgag atcatggaag
    22861 taattgtcaa ggactacctc tcaaatcatg gagtgaattt atgagagtaa tgggcttgga
    22921 tgtcggttct aagacagtag gtgttgctat cagtgacccc tttggctgga cagctcaagg
    22981 agtagagatc attcggatca atgaagcaga agaagagttt ggctttgatc ggttgaagga
    23041 attagtagca caatatgaag tagaccggtt tgtagttgga ttacctaaaa atatgaacaa
    23101 ttccatcgga ccaagggcag aagcttccat gtcatacgga gaaaagatca aagaactatt
    23161 ctccttgcca gttgattatc aagatgaacg attaaccact gttcaggcag aacgtatgct
    23221 agtagagcag gcaaatactt caagagccaa acgaaaaaaa gtcatcgata aattagcagc
    23281 tgtgatgatc ttacaaaatt atttagacgg ctcaggtaag caattatttt aataataaaa
    23341 gaaaagaggt aatcttatga cagaacataa ccattcccac gaccatgaag gacacgaaca
    23401 catcacattg gtggatgacc aaggaaatga aacattatat gaaatcctac taacagttga
    23461 cggacaagaa gaatttggac gtaactatgt tcttttatat cctgctggtg tttcagaaga
    23521 tgaagatgta gaattacaag cttatgcata catcgaaaac gaagatggaa cagaaggcga
    23581 gttagaacaa atcgaaacag acgcagaatg ggacatgatt gaagaagtat tcaatacatt
    23641 catggcggaa gaagaagaat agaacaaaag aatgaattaa taagatataa aaaaagggat
    23701 tgtgacaaac gtcttgtcac aatccctttt tccaaataaa cgatgttcaa tcagctgccc
    23761 ctcggcattc gctcgaaaaa cggcgaaata tgaagaacta ttttgccatt tgtttctcta
    23821 ataatcaggt caaaacgttg acttcacagt ctcttcctta aacggtgttc tcctgccaac
    23881 tcctcggtca caagtcacaa tattcaacaa acatgagaag ctgttttttg aaattgttcc
    23941 ttgtgactcg gagctgaacg gcaggctcac gacctctttc ttaaacggtg ttcaaaagct
    24001 tgcatctgcg gtaataagtt caaccaaacg aaaattatga ggagctattt ttgtttggct
    24061 gttcttatta cttgatgcag gacagctttt tcacaacctc ttttcgttta gcactaaatt
    24121 tccttaaaaa gtagaaaagc tttcgaagcc tcgtttgttt cttttactgt ataataaaag
    24181 aaaagagtaa caatcgctaa aagatataaa caagagaaag gaaatacaag atgattggtc
    24241 tgatttattt tgtggtgatc gttctggcga acacagttgg cgctatttcc gggatgggtg
    24301 gaggcgtgtt gatcaaaccg attcttgatc ttatcggcgc ccattctgtt gcagggattt
    24361 ctttttactc aacagttgcg gtattcacga tgtccattgt ttcgactgtg cgacaagtat
    24421 caagcgggaa atcactgaat tggcagattg tcggttgggt atctagtgga gctgttgttg
    24481 gaggagtagt cggcaacatt gtattcgaag tttttttaca gttatttgaa aacgagaagc
    24541 atgtgcagct gatccaaatt tttttgacgg tattgacctt ggtttttgcg ttcttctaca
    24601 ccaagcatca tcagccaaaa tttcatttga cgtcatggac ttggtacctt atatgtgggg
    24661 gggttctggg ctttttagct agttttttag gtattggtgg tggaccagtc aatgtttctt
    24721 tgttgatgct gatgtttgca ttgccaataa aagaagcaac actatattct ctatctacga
    24781 ttttcttttc tcaactggca aaattggtaa cgattgcttt aacaagtagc tttatgcgct
    24841 ttgatttatc tatgttgttt tatgttatcc ccgctgctgt tattggaggt ttgtggggag
    24901 caagatttag cagggttttg tctccaaaaa aagtgacctt tatttttcaa gtaattgtcc
    24961 tcgtggttct tttggtcaat tgttacaatg cctttgtcat cttacaaaca ttaggagaat
    25021 ctagactaac gcaagtcaaa atcactgaaa catattgttc taaatgaaaa aaagagtata
    25081 ctatgataaa aaaattcatt ttaaaaaatc agaaattggc tggtgatttg gatgttacgg
    25141 gaaatgcgtt tagaagatgc agaagcacta cgtgaaatca atgcggatca attagggtat
    25201 gatataccat taaccgttac ggtgagacaa atgaagaaac tgctgaagga gccagagaaa
    25261 aattttttct tggtttatga agatcctact tctaaaaaag cagctggcta tgtacaagcg
    25321 gagtcctatg aatcgatttt tagcgatcca atgttcaata ttatggcact tgctgtgtct
    25381 aaatatgctg aaaaaagagg catcggaaag gctttgatga ctggcgtaga agaagcgaaa
    25441 aaacgaggaa taacgacaat aaggcttaat tctgcagaat acagagtaga agcacaccaa
    25501 ttttatgaac acatcggtta tcattccgat aaaatgcaaa aaagattttt gaaaatcctg
    25561 taaatcttta tcaaaaaaat acccgatcac caagccgata cttgatgatc aggtattttt
    25621 gtttttttga ctaagaaagt aattgcatca agaaaccaga gatgatcaaa acgatggcac
    25681 caccaagtcg attgcccatt tgagcaaaag caataagttc catccgatta gctgcagata
    25741 agacggctac atttcctgtt ccgcccatcg agttgttgca taagccagca gtaatagttg
    25801 attcaattgg atataaacca aacaaacgac cgacaaatcc tgaaacaact gaaatcgcga
    25861 taacacttgt cagacataag acaacaaatt gccatgtcaa agcagaagct aatacattca
    25921 gattcagcaa cgcaataccg ataccggcaa gaacagctgc tgtcaagttt ttgacaatca
    25981 agttattgaa catgatagct gcatcttcat aatactctgg taataggttg gcgattttac
    26041 agatgacaac gataataatc ataaatgcgt aagcatggac tttaggaaca aaatagttac
    26101 caatttgtcc taagatgaag aaagtcaatg ataccatcaa accaacacct aactgtggaa
    26161 tggaaggttt cacagttcga ggttttccat cgccaagatc gcctttttcc attaatttac
    26221 cattgccgtt gaattttggc attgattgtc cagcacgagc aactaatgct gcaccgatga
    26281 tagctaagac atttgtcatt gctgtcgcag gaatcaggcg ggaaatgata gaagaagcat
    26341 ccgtacctaa aacatttgca taaatcgttg ataaaggaac ggctccggct ccaacaccac
    26401 cagccatcgc aggtaatgag atgtataaaa cggaatttgc aaaaccattt ccgataagca
    26461 tgcccacgag tcctacggca aaaaaggaga cgaccattga aataagtgca acgggaataa
    26521 aacgaacaga agctttcatc aatagattac ggttcatacc aaggatgcta ccagtaatca
    26581 aggcagcaat ataaaagtcc aaaaagccca tattgctgac aaagttttct gtggatttca
    26641 ctacatattc aggtaagatc ccaaatgtag caatcgcagc agaagcgaag atcgcaaaaa
    26701 cggaaccacc acccaaataa cttttgacga tagggatttt tgtcccgata aaatgaaata
    26761 aattaccgaa aataaccaaa actgcgattg gtccaatcat attcgtcggt aatttcccta
    26821 aagccattgc aatgatcagg acaaggacca tgatcaaata aagtggcaaa cttacgccgg
    26881 tgatttttgt ggaccaaaaa ccttcttttt tcttttcctc aactgcagtt gcagttgttg
    26941 ttttttgtgc catttttaca gatcctttct tattctgtat catatgaaaa aagccaaaga
    27001 aaagcgacaa aagagcagga gttggctggc cgctgttttg ccgcagtcgc ttttttcttt
    27061 ttagacgtca tacaaagttt ctgtatgttc ttttactata acgatcttaa tcttctaaag
    27121 atttatattc aggaacccat ttagcagatt caactgcttc tttgatatcc gtgatttctt
    27181 cacgattcaa gttttgtgcg acaactgatt ttgcgacagt ttcagcaatc gtttgagaaa
    27241 attcagtgat ttttgctact ggaggcaaga tagctgctcc tggttttgtt acatcaacga
    27301 ttccgcctaa tgctcgacta gcttgagaaa tgatttcagc atttactcgt gtagctgttg
    27361 aagcaatcaa accaagtcct aaacctgggt acatcaaagc attatttgct tgaccgattt
    27421 ggtaagttac accattatat tcaacatcag cagcagggat acctgttcca actaatgctt
    27481 ttccgtccgt ccattctatt aaatcttttg cttttgcttc tgccaatttt gttggattag
    27541 acaaaggcat gatgatcgga cgtggtgtat gagcagccat ttctttcacg atttcttcag
    27601 tgaatgctcc aggttgggta gaagtaccaa tcatgattgt cggatggatc gcttttacaa
    27661 ctgcttctaa gtttgtcaat tcatcagcat tagtgaaatc tgagcgtttg cgagcgaatg
    27721 ggatttgacc aggagtcaat ccttctgtat cttcaaacaa taaaccttgt ttatctactt
    27781 gatagaaatg ttggcgtgct tcttcttcag gtacaccttg acggatcatc tcatctaaaa
    27841 ggatgtttgc gatacccaca ccagcagtac cagcaccaaa tgtcaaaatt atttgatctt
    27901 tcaattgttc accggatata ttcaatccgc ctaatacacc tgctaggaca acaattcctg
    27961 taccttggat atcgtcgttg aaagtagtga tttgatcttc gtatttttct aagatcgctg
    28021 cagcatttcc tcgtccaaaa tcttcccaat gcaataacaa ttctgggaat aattcggtag
    28081 ctgattgaac gaattgatcg atgaattcat agtaagtttc gccttctaca cgagcatgac
    28141 gattgcctaa gtataaggga ttattcaata attcttcatt attagtacca gcgtcgatcg
    28201 atacaggcaa aacttgtgct ggattaatac cagcagcagc agtatagacc atcaatttgc
    28261 cgatagcgat gtcaacaccg ttcacacccc agtcgcccat acctaagatt ccttctgcat
    28321 cagtcacaac aatcaaacga atgtctcgtc catctgccgc gttcttcaaa cttgctttga
    28381 tgttttctgg ttcgtcgatt gaaagaaatg cagcatcttg cggtttcaag aaaatttcgt
    28441 tgtattgttc gattgaatcc gcaacgacag gatcataaac gacaggcatg aattcaacta
    28501 aatgttcgcc catcaattta taaaacaatg tccgattcgt attgaataga ttcattaaga
    28561 agatacgttt ttccaaatct gtttgtttgc ttaagtattg accataagct tgaacagatt
    28621 gttgttccag tgtttggaca gtacttggaa gcataccagt gattccatat tttgcccgtt
    28681 cttcttttgt aaaagctgtt cctttattta gaaaaggatt atttaataag tttaaccctg
    28741 taagcatgaa tgattcctcc taagttttaa tacaaacgaa gtatagaagt cagatgatga
    28801 tatagtcaaa aagagcaggt atgataaatt ttatttatat gtaatatagc caaaagaaat
    28861 caaaatagca gtatttgttc tttttatatc gacaatcctt gcttgaatac tatgcgtatc
    28921 tcttatatgc tagagaaaga aaagtagaag aataagggat agatggaatg gataaatagg
    28981 atttctgagg aaaaaagcac atgatagcgt tgataggagt cacctatgtc aggtgataaa
    29041 aatacataag tagaaaatta agaggaggaa gagaaaatga aagaagttca attgattaga
    29101 aaaagcgaac tatcagaagg cggttgcaat gcatgtggcg tagtagaagc gacaagctat
    29161 acattaaaat tagattccaa tcaagcaata atctctgaac tgacagtagg cggtcttgtg
    29221 gacagtctgg cgcttgctga aggttttact ggagaagata tttatgagat gttttcagaa
    29281 gtccgtcagt taaaaaaagg agaaaaatgc atagaggttc atcatgagag cccgaatgtg
    29341 aggttcaaac gtggtgataa tgagatgatc ttcaagaatc atgtgtctga tcacactgaa
    29401 ctatatgaaa tcgtgaatca aatactgtct gggatttttg aattaggacc ttatgagttc
    29461 aaggaagaaa acggtaatcc aaaattaaat gaagaatggc aagaaacgat tgaaacacaa
    29521 agaaacaatc cacacttatt tcaatagtct tccacagtat ttcgtaaaaa tgttgatgaa
    29581 aaagaaacac cgtgaatcag aggtcgtgaa aaacctgccc tgcatcaagt aataagaaca
    29641 gccaaacaaa aatagctcct cctatttttc gtttggttgg gcttattacc gcagatgcaa
    29701 gtttttgaac accgtttaag gaagagactg tgaagtcaac gttctgacct gagtattaga
    29761 gagacaaatg gcaaaatagt tcctcatatt ttgccatttt ttgagctaat accgagggtc
    29821 agctgattga acaccgtttt atcgagaggt tgtgaaaaag tcgcttagtt tcgagtagta
    29881 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttgactt aataccgaga
    29941 aaatggcttt tgaacaccgt ttattcaaaa aaagggactt tgacactact tttgtcaaag
    30001 tcccatcggt tttttatcta tttttattac aatcatttct tccagagtag tgacatctgg
    30061 aatggctgaa atgtaaacag attatttgcc tgtatcaatg tcgaagagta atataaaaag
    30121 tgtaaacgct atcatattgt tcatcattca aattttatag agagaaggca ttgagatgga
    30181 aaaaacatat caagcaggta caaatgaagg aatagtagat tttatcaata tggaagattt
    30241 agaaatcgcg gcttcacaag tgattccagc tggtggttat ggttacatca gcagtggtgc
    30301 tggcgactta tttacttatc aagaaaatga acgggcattc aatcatcgtt tgattattcc
    30361 tcatgtatta agagatgtag aattacccga tacaactaca cattttgacg aagaaatgct
    30421 gactgcacca atcatcatgg caccagttgc tgctcatgga ttagctcatg tgaaggcgga
    30481 gaaagcttct gcaaaaggtg tagcagattt tgggacgatc tatacagcaa gttcttacgc
    30541 ttcttgtact ttagaagaga taagagaagc aggcggggag aaagcaccgc aatggtttca
    30601 attttatatg agcaaagata atggaatcaa tttggatatt cttgaagtgg ccaagcgtaa
    30661 tggagcaaaa gcaatcgtat tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga
    30721 ccgcagaaac gggtttacct tcccgctgcc aatgccaatc gttcaggctt atcaatcagg
    30781 agtaggccaa acgatggatg ctgtatataa atcttccaaa caaaaactta gtccaaaaga
    30841 tgtagaattt atcgctgctc attctgattt gccagtctac gtgaaaggtg ttcaatcaga
    30901 agaagatgtc tatcgatcat tagaatctgg tgctggtgga atttgggtat ctaaccatgg
    30961 tggccgtcaa ttagatgggg ggcctgctgc gtttgattcg ttgcaatatg tggcagaagc
    31021 agtagataaa cgtgtaccaa tcgtgtttga cagcggtgta cgacgtggac aacatgtttt
    31081 caaagcaatc gcatcaggtg ccgatcttgt tgctattggc agacccgtta tttatggatt
    31141 gtctttagga ggaagtacag gagtccatca agtgtttgat ttctttaaaa cagaattgga
    31201 aatggtcatg caattagctg gaacacaaac agtagaagat atcaaaaaaa taaaattgcg
    31261 tgagaatcgt tttatttaag aaataaatga cgaaaaaaat aggagtgcgg cagatgatgc
    31321 cgtactccta tttttatagt tcaaggatgt cactaataga ataatttgaa acgaaaaaat
    31381 aaaattgaaa tattttttca tacttaataa aaaaggaaga atattcatgt aaacgatttt
    31441 attgtttttt tctcttttat acaaaaaggg tcataatttc aagtaagcgg atgaattcat
    31501 gaggattttt tgcatactgt ctctactata cttaggtcac taagaaaaag gagtgattta
    31561 ttatgaaaaa gaacatgatt gggacattat tactttcaac acttcttcta ggcggcttgg
    31621 caacaccagc ttttgctgag ggtcaagcga cttcaaaagg agatattacc tttactgaac
    31681 caacaaatac cgttgagcct cttaacccta ctgatccttc aaaaccagta gagccagcag
    31741 atccggaaaa tccagcaact ggccagacag gttctttaac attggatgta gttcctgaat
    31801 tgccatttgg tacacacgag attgaaagcg gaacaaaaac gtatcaagta gacgcttcaa
    31861 aaaacgatac accttatctc caagtatcag atcgtcgagg tgtaggtgca gatggccaag
    31921 cacaagggtg gaacgtgaca gtttcggttt cagattttgt aaacggttca caagttttgc
    31981 aaggcgctga attagatttt ggaacatcca ctgttaaaag tacgtcagat aacgaatcca
    32041 ctggaccaac tagtcaaact gtaactgggt tgagtaaagc aagtgcagcg acaccaatat
    32101 ttacagcagc aaaagatcaa ggtctgggaa catggctttc tgtttatgat ccagcaaata
    32161 tcacactgaa agtgccgaaa gcagcagctg gaacattcac ggctgatttg acttggaacc
    32221 tcgtggctgg cccagtagca tagagtatga aataaattca acaaactgcg gtgaaaatac
    32281 tataggtgaa ggatttttta ccgcagttgt tgttgaaagg atgtggtgga caatgaatca
    32341 aagtaaacga ctgtttgttt cgtttttttc tattttaagt ctatttttca tttttccgtc
    32401 aatcagccaa gcaaaggatt cagcaggaga ctttggaatc aaaccggtat ttccagagaa
    32461 ccaaatagat aaagcaatcg gttattttga tttattagtg gcaccggaac aaaatcagac
    32521 actagaagta attatcagca attcttcaga tgaagaacga acatttgagg tctctgtcaa
    32581 tccggcggta acgagtgatg gtggaacgat cgattatagt caaaaaaacc caacgttaga
    32641 tgagacattg ccgtttgatg tccgtgacgt tttgctcatt gcgaaaaagg aaatcaatgt
    32701 atcagcacat gcagaaacaa ctatacctat agaagtgaaa atcccagcaa aaagctttaa
    32761 gggacgtgta ttagcgggta ttcatgtttc acctaaagaa gaagctgaaa cagaaaatgc
    32821 tacagaagga gcacaaatca aaaatcgtat tgcttataat ctcgcagtcg ttttacaaga
    32881 aagtcaagag acgatagaac cggatctgaa acttttatca ggagatttgg acgaggtcaa
    32941 cgcaaagcca actgttcagc ttcgtttcca aaatcctcag cctaggatta tcagcaattt
    33001 gatatttacg agcaagatct tttatgaaaa tcagctttat attgaaaata cgtctaatga
    33061 atttttagtt gcaccaaatt cgaattttca cttgaatctt gatttagctg gtgataaagc
    33121 aaaagccggc gattaccgcg cagaaatcat agcaaaatca ggcgacagta atgaatggcg
    33181 atttacacaa aactttacga tcaaaaaaga aaaagcacaa aaagtaaatg aaaattccgt
    33241 ttttgcagta caagaacaat ctttcccatg gctttatgca ggattggttg ctgcagttct
    33301 agtgttatgg ttgattttgc tgtttttgtg ggtccacaag aaaaagaaaa aaggagaaac
    33361 aacatgatga ggtgatttcg ttgaaaacat cgatcttatt gggagggatt attgcaagtt
    33421 tgctgttctt cagctggcaa gagccagtcc aagcagatac caaaagttca ggtacgatta
    33481 cattttatga tctaagtcca gttccgcatc caagtggttc agtcaatggc gcatcaaaac
    33541 agcagacaca gctaccgatt tataaagaat ctaatcaaca tggcgtattc ccggagacga
    33601 atgaagaaaa gatccgtttt ctttatttga tcattggtat agaattatgg cttcttgcgt
    33661 ggttgttgtt atggagaaga aaggaaaagg aggatgaaca tgttgctaca aaataaattt
    33721 tcattttttc gctccttctt ttttacttcg ctttttgttt taagtatatt tatcttttca
    33781 ccttcggcag aagctgttgg agcaacgcct cctgccattt cttatgatcc atacacgtat
    33841 gaaaatatcg tacctccaaa tggtggacgt agtgcagtca atggcgggaa gcttgtccaa
    33901 ctaactgggg ctaaccaatt gcctaaattg actgctactt atttgaacga tggttcaaat
    33961 gatccttgga aaggtggcac ggtccgtttt cattgggaaa gccatgtgaa actaacaaat
    34021 gtacttgatt tggtgaatat cactgcacaa ggatttgcat ttgtggcgat catcaatttg
    34081 ccggaacatg tatcaagcag tgatgtatta aaagcaattg actggaacaa cgcctacttg
    34141 tatattgccg gtgcccccat caaggttact agcggtagtt tattatcagc aggaagccat
    34201 accttgcgtt ttgggatggg gtcgtgggat acaaatactc tattggcaga tattattgct
    34261 attattacgc gaggcgaatt caacgtggac gatatcccgc ttcaatttga tgttacagct
    34321 aatgtcgcga agatgacaga aaacggagat aaatatgata cgagtccaaa caaaattttg
    34381 accaagggta aattacaacc ttctatgtct aaaaaggccg ttttttctgt tgatttttat
    34441 gattcgaata atattatcca agatcgtatg ttcaattcga ttttagctcc agctgcaggt
    34501 agattttatc ctaaattaac tggcggaaac ttgaatccag gagcatatgc attttctgca
    34561 agtgcagaga taaatacgtg gaatagttac atctcaacaa gtgattcaac aggaaaatat
    34621 aatacatttg ctgataccac agaatacgtg gatggaaata accagaacct tccaggctct
    34681 accaatagtc ggacattgac gatggggctg actgaaggac gttttgatca gttgccattt
    34741 agtcgctttg atcgcgtagt caactatttt acaggaaaaa acgttacaca aggagcaata
    34801 ctgactcata ctccagcaag cacaagtgga ttgaatcaga cagccaatat cgtgtacagt
    34861 gggaaagatg cgcaaggcgg attgctttct cctgttgctt taaaggtatt ggagcagtac
    34921 aagctagacg gaacggtttt accaacaaac ttaacagccg atagtcagtg gggaaagctc
    34981 ctttcttctg catcgtacca agtcacggcc aattggtcag caactgaact aaaaaagggt
    35041 tcgatccatt acaggcttta ttcgaaagga acccaaaaat tagcgacagg atggacagat
    35101 caacttttcc aaacgatcca atcaaataac gggggtgtaa atacagctac ttctacacta
    35161 cctgcattgg cttcagggca atattatttt gattatcgct tggtggacga tgtacttact
    35221 aacatgtatc cgtcttttgc gtacaaatgg cagtctgaac aagctggaat caccactctt
    35281 cctcaaataa cagtggctaa ttttccatct gtgagtgcag atagtcagct aaaaaacctt
    35341 agcagacaac ctgagacagg agatcccctg tctgctctat caggagatac gattcaagaa
    35401 aatgtgacat tcacgttaac aaaatctggg gatgcgataa ccgacaaaaa aatcacgatc
    35461 tcacttcctg aaaatactac ttatatcaat ggttccttaa aattaaatgg tacaactatc
    35521 gcagataccg gcattcaaca aggagtttct gtcccagcag atctattgag caaaatagga
    35581 gataccattc atcttactta caactatcaa ttaaatactg tagatacttc tgtacaatcc
    35641 gtcagcatac tgacaaaggc agctgtactt tccagcaata ttacattagc agatggtgct
    35701 aagcttccta acccagttgt ccaaacatct gctaaaacaa ttcttgtccc caaacaagaa
    35761 ttaacattag ttaatgtacc agatgatttt acatttggta acgatctccc caaaccgttg
    35821 aagacaagtt attatgaagc aaaaggcgat ttttcttttg atgtgcggga tactcggctt
    35881 ccaagcacgt ctccttggca actgacaggg acattgactt ctttatttaa gaatgatcaa
    35941 ggtcaagaat taagcggaac aaaattatac ttcaatcatt ctggatcaaa acaattgatt
    36001 cagcaaggtc aaaacacgtt gatttatgag agtgatggaa cagctaaagg agaggttctc
    36061 gttgactttc ccgataccga tggtttactc ttagaggtaa atagcagtac gaatgctcaa
    36121 cctggcgcaa cgtatcaagg catggtcaca tgggaactga cagctgggcc aaccagctaa
    36181 gtgattagaa tttgtatgga tctgtgataa ctgtcacaga tcctcttttt caaggatgag
    36241 aaggaaactt ctgctgttaa aatcagaaat gcttggagga gtggacttgg aacttttcag
    36301 tttgcttgaa aaaaatgaac aacggcaaat caaacttttc caacttttat tagaggaaaa
    36361 aggagagatt tccgttaata acgtacttac ccgcttatca gttgatcggg ctaccttgaa
    36421 agaagatctg ttttatttac aagctaactt ggaaaatatc aaacaattgg aaattgttat
    36481 ccaagaaggg aatattttat taatcagaca tggaaatata gcagcagtcg atgtttatta
    36541 tcattgctac ttgcgctatt ctacaaaata tcagatactt ctttatctgt tggaaaatgg
    36601 ctcatttgat cggcagaaac ttgtcatgga acttaatatc agttcggcta ctttgtctcg
    36661 cagagtgaag gaattaaatg attctttaaa tgagtttcat ctgaaaataa aaagcgggcg
    36721 gctgaccggt ccagaaagtc aaatttgtta tttttatttc caattatttt ggtttggacg
    36781 tccttatctt gaaaacaaaa aagaatacag tgatttatca ggaaacactt ttttagatct
    36841 gctgcaaaaa gagtttagtt ttactattac agaagaagga cgattgaaat ttgaaatatg
    36901 gctttccatt atgaagaagc gaatgcgctg tacgaaaaaa tccaaaccag attatagcaa
    36961 tcttcctgtt ggattgtttc aaacgacaca atttttattg gacctccata catttttgac
    37021 ccgttacttc tttcaatatg cctttccttg gaatcaaaat gaaacgttgg ttttttatct
    37081 tttcatgatc agtaacttta cattagatac acataactcg catgtccagg agtttttgat
    37141 aagcgaaaaa tacaaagacg acgtagtagc tcaattgaat cgaacgttta aacaatcgat
    37201 agaagaatat ttctcttttt atgtttttcc agaagagatt tcgcaaaaga ttgcgttgac
    37261 agtgatgcaa aatcacgctc attttgccta ttttagagga tggatgaata tttttggcca
    37321 agaaggatta aaacagcggt taactgaagt agaagataaa caattcctgg ttttatgtga
    37381 aagattggtc agacaaacat ttgctattgt tgattttact gaagtagacg aggaaaatgc
    37441 ccgctttgaa ttatttgctc gctatgcatc tattttgcgg acgctgatca agcagttaga
    37501 attacgtctg caaattgctt atgatcttcc ttatgaaagg ctaatggcgg atatcgtgaa
    37561 agaaatcatc caggaacaat tgctgaatac gatcaaatat gatttagtcg aatacgagaa
    37621 agaaaagcag tatgatatca ttttgactag ccaattgaaa gagtacccgt cacagaagtc
    37681 cgctaaagtt tttgtattca ctagcaacga atataattat gatttcccct atttgaacca
    37741 atttttaaaa gaatgttatt tagaaaaatt gaatttaagg atgaataaaa catgaaaatc
    37801 gtgatcattg gtgcttccca tgcaggcatc acagctgccc taaatctaag aaaacttcaa
    37861 ccgaaagcag aagttttact gatcgatgaa gatcataaag atggactagg ctatgtttca
    37921 aatggaatca acctttattt aaaaggaaag atacgtagtt tagctgaagt tgcccataac
    37981 atgcggactc ttcaagaaag tggggcaaaa ctgatcaccg aatggagagt aactgaactt
    38041 gatccagaca aacatcagct catcctcgca tccaaagaag gaaaaaacga gaccataacc
    38101 tatgataaat taatcgtagc aacaggttcc tctcctgtca ccttgtataa acaaattgaa
    38161 gctgaaaatg tctacaccta taaaaatcta gtgcaatcta agcaagtact tgccgcttta
    38221 aaagaagcaa aagaagtagt gatcttcggt gctggttata ttggattgga attagcagat
    38281 gctttgagaa ataaaggata cattattcat ctagttgatt atatgccgaa tgttttatca
    38341 agatatttcg ataaggatat gatcaacagt tttcaaaatc agctgcaaac taagcaaatc
    38401 aatttttatc caaatgaatt cttgatcgat tggaaaaaat cagaagagaa agtagtatcc
    38461 gtacaacttc tatctcaagc aataaaggca gatatggtca ttttttctgc ccaaactcgt
    38521 cctaatacga cattgttaaa agaaaaagtg gctttgtatg aggatgagac tgtgatagtc
    38581 aatgaatatc tgcagacaag tgatccggac atctatgcga ttggagatat cgtcccagtc
    38641 agttttgaca aaaataaacg gcatctcttt ttacctttag ttactcgagc tgtacatatg
    38701 gcaagagcag tagcgctaac tttgagtggt cagccaaccg cttacgacct cagacaaaag
    38761 ataacagcag cagtcattat agactacttt ttaggaactg tagggctgac agaagatgaa
    38821 gcgccattct tggaacaaaa taccggttcg tgttcaggag aatttgatct atttccacaa
    38881 tacgatgaag aaaacaaaac agtaaatgca aagctgatct accatccgga tacattagaa
    38941 ataattggcg gacagctgat aagccaagaa ttcttattat cagatttgaa cctgcttgcg
    39001 gatatcgtga aacacaaaac tacgattcca cagctagcag ttgaggattt tggatttctg
    39061 tctgaacaca cctctcgttt tcactacctc aatgagttag cttttaaagt actagctaag
    39121 agagcaaata gcaatagagt agataaaaga ctgtgagaaa tatcacggtc ttttattgtg
    39181 tggggatata ataaatatta atcaatcgag aataaaaaat tcatataatg aaatagcgga
    39241 agaatttttt gagcataata ttcgccaaaa atttatcaag tgataaaatt gttgtataaa
    39301 atgattattt caggaggagg agttaaatgt ttttagcatt aaatgaaatt atgcattcaa
    39361 agttacgtta tgcgttagtt gcaggggtga tgtttttgat cgcctacctg gtattttttt
    39421 tgactgggtt agcttatggc ttagctcaag ataatcgaac agcagtcgac aagtgggagg
    39481 cagattcgat tgtgctttcg aaagatgcaa attcaaatct agggatgtcc atgattacta
    39541 agaagatagc agaagaagtc gaaggtggga aagtggcgta tcttgcccag acacctggag
    39601 tcgtgactag taaagacagt acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg
    39661 ataaaaatca attcatcatg cccaatcttg ttgaaggaaa agcttttgat aacgatgatg
    39721 aggcagtagg cgatattagt ttgaaagaag aatatggtct agccgtagga gatacagtta
    39781 agttatctgg tagtgacaag acgtttaaac taactggctt tacggatcat gccaaattca
    39841 atgtctcacc agttctctat acaaccatca atgcttacca agagatccgc ttcgaaaaag
    39901 aagatacaag tgaaaatgca cggattaacg caatagttgt cagaggtaaa atcagtaatt
    39961 tacctgaaga tttagaacag atcaaaattt ctaaattcat caatgaatta cctggatata
    40021 atgcgcaagt attgactttt ggatttatga tcggattttt gatcgttatt gcggctattg
    40081 tgatcgggat tttcatttat gtactgacga tgcaaaagat taatatcttt ggtgtaatga
    40141 aagcacaagg gatcacagga ggatttattg cgcgttctgt agtggcacaa acattcattt
    40201 tatcatttgt tggtattctt cttggtctat taggaacagt gggaacatcc cttgttctac
    40261 ctgatgcggt tccattccaa agcaattggc tgttttttgg cgtcattagc ttgttgatgc
    40321 tggtagtagc tgtgttaggc gcactatttt ctgttcggac aatcgtaaaa atcgatccgt
    40381 tgaaggcaat tggataagaa ggaggaagag catgaaagca atagaattta aagaagtaga
    40441 aaaaagtttc caagatggcg accaagtagt taaagcattg aaagaaacaa ccatgagtat
    40501 cgataaaggg gaatttgttg caatcatcgg tccttctggt tcaggtaaaa gtactttttt
    40561 gactttggca ggcggattgc agacgcctag cagtggctct gtatttatca atgatcaaga
    40621 attcagtaat caaaaagaaa gtgaacgcgt taaactacgt tttaaagaaa ttggctttat
    40681 tttgcaagct tccaatctgg ttccttttct gacagtaaaa aagcagttat tattagtaga
    40741 taagatcaaa catgaacatc gtcaaaaaga agcaaaagaa ttatttgagc agcttggcgt
    40801 ggataagttg atcaacaagt atccagaaga actatctggt ggagaacggc aaagggtcgc
    40861 aatcgctcgt gcattgtaca atgatccgtc cattattcta gcagatgaac caactgctag
    40921 tttggattca gaaaaggcac gagaagtagt cgatatcttg gcaaaagaat caaaagacaa
    40981 aaataaagca acgatcatgg ttactcacga tacacggctg attgacaaat gcgatagaat
    41041 atttatgatg gaagatggtg tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa
    41101 tggtccatcg atacaacaaa gatacacagc aaaaaatttt gaaagaagcg actgacttgt
    41161 ttatgtccaa gggatatctc ggcacttcaa caagagagat cgcacaaaaa gttgggatca
    41221 cccaacccaa tctctatcat tatttcggtg ataaagaaaa actatatacg gcagttttag
    41281 aaaaccactt aaaagatgtt ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg
    41341 gatttcaagt aaccttgaca aaaatggcgc agtttttgat tgagacacat ctggtcgatt
    41401 tgtttctgat gcttcacgat cttgaaagca atctttcaaa ggaaacaaga gatcatcttt
    41461 tctttctatg gaagaaaaat tatcgtgaac cttttgaaga aatcttttca gcaaaccaat
    41521 ctgtgctaag aaatgggatt tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag
    41581 caccttacat cactcggtcg aaaagacctg tggaagaacc gctaaatgtg gcgcaattga
    41641 tcgatttata tttgaatggt gtattaggag aagcgtaaag ctgttatata gggagaaaaa
    41701 tagttaggtg aagaagcaag atatgacgaa gatcgttata tcttgttttt tttatatagg
    41761 tagtttttca taaatacaaa tttgttattc tctcatgatt atcagggtat atttttattt
    41821 attatgtcgg tgttccagga agtgttttaa tttaataacg aagaaattaa ttgtttgtag
    41881 aatgaaggaa actatttatt gggtagatga atcaccacga atagtcaaaa ggagatttca
    41941 ggatatgcca aaaatgaaaa aaatcatttt agcagtaatg ttattttcta cggttatatc
    42001 ttttacagcc ccttttttgc gtacttaagc tgattttaag ttttgaccct tagtctggat
    42061 ctacgaggtg agagtcaaga atgaaaaaaa taattggagc ttttgtattt gcaatcatta
    42121 tcattattat ctgttttcaa atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg
    42181 tcagcagtac gaacagtacg gttacaaaga caaaggaatc atttgatgat acagaacaag
    42241 agacattaat caaaatcgga aaaaatgacc gtacactata tacagatgac tcgctaacta
    42301 ccccacttgc aactataaac ggcggagagc tggcggagtt tctttctgaa acaaaaaatg
    42361 cttatcagat caagacaaat gatggctata ctggatattt agccctcgaa gatggaacca
    42421 aagtcacaaa gaatatacaa acaaaaccgg aagagctatc tgatgcagtg attgtattag
    42481 atcctgggca tggcggaaat gatactgggg cattaagcaa tgatgaacaa acagaagaaa
    42541 aagatataac attaagtaca gccataaaag taaaaaaggc gctagaggat gctggagcaa
    42601 cggtttattt gacccatact acagatgaat tagtacagct tggagatata tgcgactaca
    42661 gtgaagaaaa aaaggcagat gtctttatca gtttacacgc agactctacg gaatatgcga
    42721 atgaagcaac tggaatcacg acatattatt actatggaca agaggaaaca ttagcacaga
    42781 caattgctga ctccttcact gatctgcctc tggattcaag aggaatctcc actgggaact
    42841 atcaagttct tcgagaaaat cttcaacctt ctattttaat cgagatgggt tatatgaata
    42901 atgatagtga tttaaaggaa ttagtaacag acagttatca gcagcagatt gctgcaagtt
    42961 tgacacaagc attgaccact tattttcagt aataaataag cccagccaaa caaaaatagc
    43021 tcctcatatt tttcgtttgg ccgggcttat tatcgcagat gcaagctttt gaacaccgtt
    43081 taaggaagag gttgtgagcc tgccgtttaa ctccgcgtca caaggagcaa tttcaaaaaa
    43141 cagcttctca tgtttgatga ctattgtgac ttgtgaccga ggagttggca tgagaacacc
    43201 gtttattcgg aacaagggac tgtgacatga cttttgtcac agttccatta tttttttagc
    43261 agcttcaaag ctttttctgt attggcaaaa tgaagtttgg catatttttc aagcataggc
    43321 atgccgcctt tctccaatag ttttgccgct acttggtcag atttcgttcc agctcctgat
    43381 ggaagggtga ttccagcttc agctgatgct ttgtgtaact cttccaaaaa gctggcacga
    43441 ctgatgatag aagcagcagc aacagctaaa tgatattgtt ctcctttagt aacgaagtac
    43501 agtttttctt gtaattgatt tttttctaaa cgcgcatatt ttttaaaatt tgcttccggc
    43561 gtaaactgat cgatcaaaat cgcttctggt ttttctggct cgattttttt tagcagcaaa
    43621 tggatcgctt gattgtgcaa agcgactttc atccgaacgg cattgtattt tggttggatc
    43681 tcattgtatt tttctggaga taagacaagc agtttatagg ggatggtttt ttttaagatg
    43741 tcagcaagct ggatgatttg gctatctttt aactcttttg aatctctaac accaagttta
    43801 cgtaattctg caagctgatc acgtttcaca taagcagcac atactgttag cggaccaaaa
    43861 tagctgccat ttccgacctc atcactacct aaaacggata attgactgat attttttggc
    43921 aaagaagagg ctgtttttgc tggcttggct tgtttatcct gtgcctcccc ccatttatca
    43981 gcttctattg ctgcattcgt accttgaaac atcacttttc cggatgtata agcggtaatg
    44041 gtcgtcccgt ctttttttgc agcaaagcta gtataaggaa tctgtttatt taacaaataa
    44101 gagcgatagt atgctttcat ttgactaagt tggtcgtttg ttgctttgat aatgacgttg
    44161 ctcataaaat tctcctcgtt tttatacata aaactatacc gcaactcacg acttttcgtt
    44221 ttctaagaga ttgcgagaca tagcattttt ttaaagttta tcatataata gttaagtatg
    44281 tgaaaaatat ttgtggagaa cggtgaaaga accaacttca tggtacacta aataaaactt
    44341 tcatcaaata ggaggaaatg attcgtatgg cgcacgaaaa aacacgctac aaagcagtga
    44401 ttgccaacca aacatatact attatcggac gggaaaccaa gcatcatatg gatatcgtaa
    44461 cgaagctgat caatgaacag ttagcagaat tgaaacaatt atcaccacaa atggataatg
    44521 aacaagcagc aatcttgatg gctgtcaatg cgctttcgga tcaattgaag aaacaagaaa
    44581 gaatcttgga gctggaagaa gaaacagcag aattaaagaa aaaaatgatc aaatttacag
    44641 agcttgaaaa tcgtgtgaaa cgcattgaag caatcgagaa cgaagctcgg gaagtattga
    44701 aagaaaatgg tcaagcagat cataccatta aaaatcatgt ggaagcacaa cagattttaa
    44761 acgagaaacg aaaaggacaa attaaacaaa aatcttctaa ttaagatcct tttatcaaaa
    44821 taagaaaaat ttaaaaaagt tagaagagaa aggaacgtag catgctgagt ttgctgattt
    44881 tatttttatt gctaattgcg tttttttctg gagcacgtcg cgggtttgct ttgcaaatca
    44941 tattcgcgat tggatatgta ttgtcattta ttgctgccca gcatttttac aaaccattag
    45001 caagtcattt ggaattatac attccatacc cagctgtcac cccaacatca aagttagcat
    45061 tttttgatca ggtatttgct tttcacctag atgaagcttt ttatgcagga actgcttttc
    45121 tgattatttt gctaattggc tggctgctta cccgatttgt gggggttttt gtccatggat
    45181 tgacgtatgt gccgatcttg agacaggtag actggattgc aggaggcatc ctgtcactaa
    45241 tcatggctta cgttactata tttttgattt tgcagttact tgcctttgtt ccattagatg
    45301 tggtccaaaa tcaattcggc gagcatagtt tcgctcgttt tatcgttgag agaacgccat
    45361 ttcttaccaa caagatgtat gagctttggg taacgaatat tttgaattaa ggaaaatagt
    45421 gctggaaaat atttcgtttt ttgtacagag tagtttattt aagaaggctg cgtagcgtat
    45481 gtccggcaca gctctttttt catagtgaaa gataaggtga taaaatgaat aaccgaatcc
    45541 ttgaaacatt ggaatttgaa aaagtcaaac agatggtcag acaatttgta gtgactgcac
    45601 aaggaaaaga agaactggca gaattggtac cagtgagtga gaaacaaacg atcacaaact
    45661 ggcttcaaga aactgaggat ggattgaaag tgcaacgtct gcgaggcggg atccctatac
    45721 ctaaacttga aaatatccgt ccccatatga aacggatcga gatcggagca gacttgaatg
    45781 gagtggagct tgcacaagtt tccagagtat tgtctacgac aagcgaattg aatcgtttca
    45841 ttgatgatct ttctgacagt gaaatcgaat ttgcacgttt gtatatgtgg gcagatcagt
    45901 tagtcacgat ccctgttttg agccgccgtt taaaggaagc aatcgatgaa gacggacgcg
    45961 tgactgatga cgcttctcct gagttaaaga gcataagaca gaacattcgg cgaagtgaac
    46021 aagctgttcg tgaacagtta gatgggatcg tccgtgggaa aaatgcaaag tatttaagtg
    46081 atgcgatcat caccatgcga aatgaccgct acgttatccc agtcaaacaa gaataccgcg
    46141 gtgtatttgg cggtgtagtc cacgatcaaa gtgcttcagg acagacttta tttattgaac
    46201 caaaacaagt ggtggatttg aataatcgtt tgcgtcaata ccaaatcgct gaacgaaatg
    46261 agattcaacg tattttaagt gaactttctg ctgaacttgt tccccatcgt caagaaatca
    46321 tccacaatgc ttatgtgatt ggtaaaatgg atctgatgaa tgcaaaagca cgatttggta
    46381 aagaagtaaa agccatcgtg ccgggaatca gtgaagacaa tcatgtcgtc ttgaaacaag
    46441 caagacatcc actgatcgat caggaaaaag tagtttctaa tgacatcaca atcggaaaag
    46501 actatcaagc catcgtcatt acaggaccta acacaggtgg gaaaacgatc acgttgaaga
    46561 ctttaggtct gttacaactg atggggcaag caggattacc gattcctgca ggtgaagaaa
    46621 gtcaaatcgg tatttttgag gaagtatttg cggatatagg agacgaacag tcgattgagc
    46681 aaagtctatc gaccttttct tctcatatga cgaacactgt agatatttta tcaaaagtca
    46741 atgaaaaaag tctggtatta tttgacgagc tcggtgcagg aacagatccg caagaaggtg
    46801 ctgctctagc aatcgcaatc ttagatgatc ttggtaaaaa gtctgcttat gtgatggcaa
    46861 caacgcatta tcctgaattg aaagtttatg gatacaatcg ggcaaatacg attaatgcca
    46921 gcatggagtt cgatgtggat acactaagtc caacgtatcg tttgctgatt ggcgttcctg
    46981 gacgaagcaa tgcgtttgaa atctctagcc gattaggtct tgatacagaa gttattgatg
    47041 aagcgaagca gttgatgaat gatgagagcc aagatttaaa cgaaatgatc acagatttag
    47101 agaatcgccg gaaaatggca gagaccgaat atctggaaat gcgtcatttt gtttctgagg
    47161 cacaagaact tcacgatgat ttgaaagaag cttacagcta tttctttgaa gaacgtgaaa
    47221 aagaaatgga aaaagcaaag aaaaaagcaa acgaagtcgt gtcagaggcg gaagaaaaag
    47281 cagaaaaaat cattgctgat attcgtaaaa tgcaacagca gattgggcaa ggaaatgtga
    47341 aagagcatca gttgatcgat gcaaagaccc aattagcaaa tctacatcaa gaagaaactt
    47401 tgaagaaaaa taaagtattg aagaaggcga aagaacaaaa aacgttgaaa ccgggagacg
    47461 aagtgttggt tacgacttac ggccaaagag ggacgctttt acgtaaaaac gggaatcagt
    47521 ggcaagtaga aatcggtata ttaaaaatga atgtgtccga agacgagctg acgcctgttg
    47581 ctcctcaaaa agagccgaca caacgagtta tccatgctgt acgatctgaa tctagtagtc
    47641 acgtgccgaa tcaattggac cttcgaggaa aacgatacga agaagcgcta agtgaggtag
    47701 atcaatattt ggattctgcc attctagctg gttatcctca agtgactatt gtccacggaa
    47761 aaggaacagg cgcacttcgc aagggaatta ctgattatct taaaaaccat cggagcgtca
    47821 aaagctttga gtttgcaccg gcaaaccaag gcggaaatgg tgcgacaatt gttaaattta
    47881 agtaaagctt actaaaggta agcagatggt tcgctatcca agtatagaaa tgctatacta
    47941 atcatagatt ttaaaaatgg aggtctttga tatgtcacaa gtaattacag ataagacatt
    48001 taatgaagaa acagataaag gacttgtatt gattgatttt tgggcaactt ggtgtggtcc
    48061 atgtcgtatg caagcaccga ttttggatca gttggaacaa gaatatgatg aagaagaatt
    48121 cagaattgct aaaatggatg tggatgaaaa tccagaaact cctcaacaat tcggtatcat
    48181 gagcattcca acattaatgt tgaaaaaaga tggtcaagta gtagaaaaag ctgttggtgt
    48241 tcattcaaaa gaacaactac gtcaaatgat cgatcaatat ttataaaaac ttacagcaaa
    48301 aataagctga gggtgggact gaatccagtt ccgtcctcgg tttttttgcg gttgagttaa
    48361 ggttattcaa attaatattt tttgagtata attacgttgt caggctggat tgtttttttg
    48421 acagacatgc tataataact ttaattgaat atgaagggga gtaccagatg aacgaacgaa
    48481 tcaaaaacaa attagcttta cttcctgatc agcccggctg ttacttgatg aaagataaaa
    48541 atggaacgat catttatgtc ggcaaagcca aaatattaaa aaatcgtgtc cgctcatatt
    48601 ttactggcag ccataatacg aaaacggaac ggcttgttag tgaaatcgaa gactttgaat
    48661 acatcgttac agaatcgaat atcgaagcat tgctcttaga gatcaatctg ataaagaaaa
    48721 atgatcctaa atacaacatt atgttaaagg atgataaaac ctatcctttc cttaagataa
    48781 cgaatgaaaa atatccccgt ttggtgatta ctcgtaaagt actaaaagac aaagcactgt
    48841 attttggacc atatcccgat gtcggtgcgg cgaatgagac aaagaagatc ctcgatcgat
    48901 tatttcctct gagaaaatgc aaacctagtc agacgaagga gccttgtctt tactatcatt
    48961 tgggacaatg tctgtgtcct tattatttcg acgtagatcc gaaagtctat acgaaaatcg
    49021 ttgatgaagt gaaaaagttt ttaaacggag gacacgaaaa aatcgagtca gagatccatc
    49081 agaaaatgga aaaagctgct gaaaacatgg agtttgaaaa agctgcagaa taccgagacc
    49141 agatccgtgc cattgagaca atcatgactc gccaaaaaat gacaaatact gatttactgg
    49201 atcgagatgt atttggttat gcgattgaca aaggatggat gtgtgtccaa gtgtttttcg
    49261 taagacaagg aaagctaatt gaacgagatg tttcaatgtt tcctttctat aacgaagcag
    49321 aagaagattt cttgacttac ctcggtcagt tttatcagga aaatgagcat ttcattccta
    49381 aagaagtctt gattcctgat acgatcgaca agcccagtgt agaagctttg ttagctacga
    49441 aagtgctgca accaaaacga ggagaaaaga aaaaattagt tgaattggcg aataagaatg
    49501 cctcagttgc tttgaatgaa cggtttgatc tgattgccag aaaacaggaa cggacgatcg
    49561 gagcagttga acgtttagga aacgcgatga atatcccgac cccaatacgt atcgagtctt
    49621 ttgataactc taatatcatg gggacgaatc ctgtggctgc gatggtcgta ttcattgatg
    49681 gaaaaccggc aagaaaagaa tatcggaaat ataaaataaa aacagtcaac ggaccggatg
    49741 attatgcatc aatgagagaa gtcatctatc gcagatattc acgagtgatt cgcgaagggt
    49801 tgcctttacc tgatttgatc ttgatcgatg gaggaaaagg acaagtagat gtggcaaaag
    49861 atgtcttaga aaatcaatta ggaatcgata ttcctattgc tggcatggca aaaaacgata
    49921 aacataaaac aaatgagttg ctattcggta tagatttggc tgtagtaccg cttgagcgaa
    49981 attcaccaga gtttttctta ctccagcgaa tccaagatga agtccatcga ttcgcaatca
    50041 ctttccatcg gcagctacgc agtaaaaaca gttttgcttc ccgattagat gaaattgaag
    50101 ggttaggacc aaaacgaaaa aaaatgctgc ttaaagagtt caaatcgttg aaaaatatca
    50161 cagcagccac gacagaagaa ttacaagaaa tcggactgcc gaaaaatgtc gcacaaaacg
    50221 tatttgataa attacatcag gaatgaggaa agggcacttt acaaagaaag taaagtgtcc
    50281 tatcatttta tgtacaacaa aaagagcgtt tacgttttgt tggtaaataa taaatcgctt
    50341 acgtctggtg tctaaggata tatgtgattt cttgaagaag gagtgaagaa atgttagaag
    50401 tcaaggatct tgttaaaact tttgggaact acacagctgt cgatcatgtc tctttccaaa
    50461 tccccgatgg aaaaatcatg gggctaattg gccaaaacgg ggctgggaag acaacgactt
    50521 ttcgtttgat cttggatttt ttgacacaag accaaggaga agtgttatgg aatggccatc
    50581 atcttgggga gaaagattat gatgtcatcg gttatcttcc ggaggaacgg ggactttatc
    50641 cgaaagtaac gattcaagac caactgcttt attttgcaga acttagaggg aaatcaagaa
    50701 aagagattga accaaaaatc gatttttgga tggaaaaatt tcaggtaaaa gggaaaaaga
    50761 cggataaagt aaaatctttg tctaaaggaa atcagcaaaa agtccagctg atcgccacgt
    50821 taatccatga acctaaattg atcattttgg atgagccatt cagcgggtta gatccggtaa
    50881 atgctgaatt gctaaaagat ggaatattgg aattgaaaaa acatggttca tgtgtcattt
    50941 tttctagcca caatatggat aatgtagaaa aaatctgcga tcatttgatc atgttgcgaa
    51001 acggaaaaat ggttttgaat ggaaaagtac atgagattcg tgaatcattt ggccgtacga
    51061 agttattctt ggaatctggt ttaagccaac aggaagtaga agaaatcgaa ggcgtgaaaa
    51121 aagtgatcct tcatgaagac aatacgttag agattacctt agaagatcct gaagctggga
    51181 aagaaatctt tactcgtgcg actcagttcg gctacattcc tatgtttaac cagcaaccgc
    51241 caactttgga agagattttc aaattgaaag caggtgaaat cgatgcgtaa gttttggatt
    51301 attaccaaag atgtctattt aaaaaatgtt aagtcgattt ctttcttgat catgatatta
    51361 gtccctttta tcatgctggg tatcatttat gtagcaggaa actttgctca acaaaatagt
    51421 gatgtcaata aaatcggcgt attggctgat aatgagcaaa tcagtcagca aatgagccaa
    51481 gtcaaaacag atgattttga gttcaaagca gttgattctg aagaaaaagc acaaaaagag
    51541 ctttcggaag aaaaaatcga tgcgtattta gtagtctcaa ctgagaatga taagataaat
    51601 gggatgcttt atagcgaaaa ttcattagga caaagtacac aactactgat acaacaacaa
    51661 ctgactggct tgcaatcgat gcttcgagca agtcaattag gcatttcacc agaagaagta
    51721 gctagcttga gccaacaagc aagtttgact cgacaaaaag tcaattttga ttcaaaagga
    51781 aaaatgacag ttggagaaga taactcgaca gtccaatatg tcgtaagtta tgtagcaacg
    51841 attgtcttgt tcattattat tttgacgtat gcgcagatca ttgcacaaga gatcgcttct
    51901 gaaaaaggca cgcggatcat ggaagtgatc ctgtccagta cgactgcaca aaagcatttt
    51961 tacggaaaac taacaggtgt tttacttgtt gcattaacgc aaatggcatt atacggtatt
    52021 atttttggta taggatacag tcaattcaaa gatatggaaa tggtcaaaaa tatattagac
    52081 ggtatctcat tggataactt gattggacca ttcttgtggt attctcttct attcatgatc
    52141 tttggcattt tgattttttc tgttttagca gctttatgtg gctctttggt caacaaagca
    52201 gaagatacag caaaagcaat tttgccagtg acttatctat ctctaggcgg atatatgctt
    52261 ggattgatac taggagcttc agatccaaac aatatcgtta ttcggatcac ttcgtatatt
    52321 ccattcttat cttcatatat tatgccggtt cgcttagcaa atgagacagt cggtatgggt
    52381 ggagcattga tttccttgtt ggtacttgta gccgtaacat tcttattgat gatcttgtct
    52441 gcaaacatgt acaaatcgaa tgtactcgtg tatagcgaag gcggattatg gagttcattg
    52501 aaacaatcga tttcaattat gagaaatgaa cgaaaaaaag cataaaatat agaaaaatat
    52561 agaaggaagc tggacattta ttgttcccag cttctttttt gtatagacct atttctattt
    52621 ttttattata ttggatatat atattcattg aaaatagagc ataacaactt tataatgttg
    52681 agtataccaa tttttggagg cgttttaatg gaccgctatt tagatttact ttcagagaag
    52741 ttccctacaa cggaagatgt catcacggaa atcatcaatc tcgaagcaat tttgcagctt
    52801 ccaaaaggaa cagagttatt tttgagcgat atacatggcg aatttcctgc ttttgatcat
    52861 attctgcgta tcggttcagg aaatcttaaa gaaaagatca aagacctttt ctctgaacaa
    52921 atgacggaaa cggagatcag ccaattcact atttttacgg cttatccaga atatgtgttg
    52981 acgactgatt ggtacaaaaa acaagataaa tcccagctga tccatcgttt gatcgatctt
    53041 ttacggttta cgacagtcaa atacactcga tcaaaagttc gaaaaggatt gccgaaagaa
    53101 tatagctata tcattgagga actcttgtat atcgatgatc gtatcaacgg gaaaaaagac
    53161 tatgtaaaaa aaatcattga acaacttgtc ttgatgaagg aagaagaaaa atttttaaca
    53221 aaacttgcgc agacgatcca aaaatctgtc atcgatcatt tgcatatcgt gggtgatatt
    53281 tttgatcgag gaactcaagc agccaaagta atggatcgaa tcatggattt ttcctctgta
    53341 gatatccagt gggggaatca tgatattttg tggattggtg cgtattgcgg ctctgaggca
    53401 tgtttattga cgcttttacg gattgccgca cgctacaact atttatttga attagagaaa
    53461 gaatatggat taaatttacg tccattattt tcttttgccc atcaaaccta tcagaagaat
    53521 ccagtattta cccctaaaaa caggaaagat ctatctgaaa gagagcaaga agaattagaa
    53581 aagatccatc aagcactttc tatcctccaa ttcaaatcag aacatcagtt gttgaatcga
    53641 cgacctgaat ttaaaatgga cgatcgtaaa ttgttggaaa aaatcaacta tgaggaatca
    53701 acgatcgttt tagaaggtca gctctatgag ttgaaaggga cttgttttca aacgatccag
    53761 ccagatgatc ctaaaaaatt cttggaagag gaacaaaaag tgatcgatag tttgatgtat
    53821 tcattccagc actcgttaag aatgcaaaaa cacatgacgt ttttgatcga aaaaggggga
    53881 atgtatctca cgaacaatca gcatgtactg ttccatggct gtatacctgt agatgaaaag
    53941 gggcagctct tagcatttga attagaccaa tcttatcgcg gaaaagaact gctgggattt
    54001 tttgaaaaac agataactga aagcgcaaaa gatttgactg caaatgaaga tttggcgacc
    54061 gatttgattt ggtatgcgtg gagcgggcca ttttcgcctt tattcggcaa gagtaaaatg
    54121 gcaacttttg agcgctattt tctcacagag cctcatacgc acgtcgaaaa aagcaatccc
    54181 tattatcact tgcgagatga agagtggttc agtgaaaaaa ttttggcaga attcgatgca
    54241 aaagaagaag gttcagcaat catcaatgga cacacgccag taaaagtaaa aaaaggagag
    54301 tcaccgatca aagcaaacgg aaaagcattt gttattgacg gaggtctatc caaagcttat
    54361 caaaaaacaa ccgggattgc tggctactca ttactatgca attcttacgg tttccaaatc
    54421 gtgacacatt atccgttctt gcctattgaa caacaatttg caaaaaaatc tgatcaaacg
    54481 aatgtaaaaa aagtgatcga gcagcagctc ccacgcaaac ttaatcggaa tacgacaaaa
    54541 ggaatggaat tacagcaaca gattactcaa ctccaaaggc tgttggatta tcgaaaggac
    54601 gattgagaaa aatgctagcc aacattgctc aatagactaa aaagcgactg caacaaaagg
    54661 cgtgtcgcag tctccttcgg aattagctca aaaaacggta aaatattaga agctattttt
    54721 ctgtttattt ctctaatact caggtcaggg cgctgatttc acgacctctt ccttaaacgg
    54781 tgttctcctg ccaactcctc ggtcacaagt cacaatatcc aacaaacatg agaagctgtt
    54841 ttttgaaatt gctccttgtg actcagagtt gaacggcagg ctcacgacct aatcagaaaa
    54901 acgattaaaa ttcattcaca aagaaatcga ataaacgctg ttcagatgaa tctacacgat
    54961 gagaaagttc cggatgccat tggacaccaa ggattttgtg catattatct ttactttgga
    55021 ctgcttcaat gagtccatcg ttggaaaagg cgatggcttt taacaaagga gaaagatctt
    55081 tgactgcctg atgatgatag gaattcacaa agtatgaatc agggagaagg tcacttaata
    55141 aactgtccga aacgattttg acttcgtgag ttgcaaactg cggggcagtg ggctgttgtt
    55201 cgtgttttac ggaccatttt ggatataatg aaagatcttg ataaagtgtt ccctccaaca
    55261 cgacattcaa tagttgaagt cctcggcaga ctgcaaaaat cggttttctt tgtttcaatg
    55321 cttccaagat caatgcctgt tcgaattggt ctcgttgaat atttgtttcc gtcagttttg
    55381 gatgagggtc ttccatgtaa agttgcggac taacatcctg accgcctgca agaatgagtt
    55441 tgtcgatttt ctcaattagt tgaggcgctg aatctggaga catgactggc agaataagag
    55501 gtaatccttg agcatctaaa actgcatcca caaatccctg cggtgtatag cttacttgat
    55561 ttccttggaa agtatctgtc gctcgaatca gttgattccc ggcaatgccg atgattggtt
    55621 tcatttttat tccccctaaa gtagactaat tgtaacaagg atttaaaaaa atttaaacta
    55681 tgtaagactt gcccctatac ttatctgttc ttgctgtgtt actatctgta aagagaattt
    55741 tcgtgtgaaa ttcatgacga tagttgaaaa tattgcttaa tttgttataa taaaaagtaa
    55801 gcagaaataa aaaggttggg cgtaagcttc aaggatctag ttcttcaagg ggtgagaagg
    55861 acattttatt gaaggggcct gcctatacta agttatcttt agaggtacat actttcaatg
    55921 aaaaaagaaa gcatgtacct cttttttaaa tcgaaaggaa gtgaagcaag tgttagaagc
    55981 tttggaacaa atccgaaaag cagaagaaga aaatgagaag cgtgctcgaa ccctacggca
    56041 gcagttgaag acctatgcag aagaaaaaga gcaagaattg gagataatcc aaacgaacag
    56101 tcggcaacag ctgaaacagt tggtagaaga aaaagaacaa ttcgaaaagc aagaattaga
    56161 aaaagaaaaa atgaaattga aagcagaagc agacaaggtg caagcaactt tgaaagacca
    56221 atatcaaaag catcagagtc aagcaatcgg tgcaatcatt gaaagggtga aagaaactta
    56281 tggccgtcac taagatggaa aaagtgaccc tcatttcaga taaaagaaat caggagagga
    56341 ttttacaagc agtacaagga atccaaaatg ttgaaatccg ggatctgtat caaaatagaa
    56401 caaacaacca gtgggtagaa aaatattttc cagataccac gattattgat aaagaagcaa
    56461 aattaagcgc tttaggcaat cggctgaaag acatccaaga agctatccaa ttcatacaac
    56521 accacggcga tagccgtcaa aagaaactcc atttgaaaca tcaagaactt tccttacaag
    56581 acttggaagc aaattattcc gaagaaaatt tcatgaataa attagaagaa gtccttgatt
    56641 taaaagaaaa gtgggaacag ctaactgaaa aacgtgatcg tttagaagaa gaggaagaat
    56701 ggcttcttca ttggcaacag ctggatattg ctccaagtat gtatgcaagt aagacgacca
    56761 tttttgagat ggggactgtg agtgcagcaa attttgaaag tttcaaggaa gaactaagta
    56821 agcttgatga cgtttatatg gaagaaatag attatacgcc aaaacaagtt tatctttcgt
    56881 atgttgcttt aaaaaaatca gttgctgcta tagaagaaat tgctgggaaa tacgggttca
    56941 ataaagaaac gtatccttat gaacaaaatc ctaaagaaaa attgcaagaa accaaacagc
    57001 aattatccat tgttcacgag cagcaaaaaa agataacgac agcgttaggt cagtgcagcg
    57061 gctatatcca agaatttgaa tgggtagaag aagtcacact tgccgttgca gaacgggaaa
    57121 aagtaaaaga acggtttatc catgccactt atctgatcgt tttgcaaggt tgggtagatg
    57181 cagaagaaaa acagaatttg atgcatgtgt taaacgaaac tgtatcagaa gaagatatat
    57241 acgttagttt cgaaacacca aactcagaag agattcagca ggaagtccct actaaattga
    57301 aaaaccatcc gcttatcgaa ccttttgaat tgctgactga aatgtacagt ctgccaaaat
    57361 atgaagaagt agatccaacg ccttggatga caccatttta tcttgtcttc ttcgggatga
    57421 tggtcgcaga tgttggttac ggattgctga tgctcattgg gacaatacta gctcaaaaac
    57481 tatttgtttt accccgcggg atgaaacgtt tcgtaaaatt ctttgagatt ctctcggtac
    57541 catcgatcat ctggggattg atctacagtt catttttcgg acaggcatta ccaaaagaac
    57601 tatttggtat ccgcttgcct ttcccaatcc tatctacgac ggatgacgtc aatacgattt
    57661 tgattttatc cgtgattttc ggtctgatac agatcttggt tggattattc gtggcagcaa
    57721 aggaaaatat caggaggaaa gattacttga gtgctgtcag tgatggtttt gcctggcaag
    57781 ggatcctgat tgggatcgtt attgcattga ttggtgtcat gttgataaaa aatacagcat
    57841 ttgtttatct tggcgcgggg ctatcgatca ttgctgcttt atgtatctta atcgtgccga
    57901 ttatccaatc gccgtctaaa gtcaagggtg ctgccaaagg agcgtataat ctttacggat
    57961 tgactggtta tatcggtgat ttagtcagtt atacacgttt gatggcacta ggtatttcag
    58021 gcggaagtat tggcgcggca ttcaatatgc tagttgcttt catgccgcca gccgctcgat
    58081 tcagtgtagg gatccttttg atcatcgctt tacacgcact gaatatgttt ttgacactgt
    58141 taagtgccta tgtccatggt gcacgattgc agtatgtcga attttttgga aaattttata
    58201 caggaggcgg acgtgccttt gacccgctga aaacggcgga aaaatacgtc aacatcaatc
    58261 ataagaaaaa aaacaataaa taattggagg atttttatac aatgatggat tacttaatta
    58321 gtcaaaatgg tggaatggta tttgcagtat tagcgatggc aacagcgacg attttttcag
    58381 ggattggttc agctaaaggt gttggaatga caggtgaggc agcagcagcg ttaacaacaa
    58441 gtcaacccga aaaattcgga caggctttga ttctgcaatt gcttccaggt acgcaagggt
    58501 tgtatggatt cgttatcgcc tttttgattt ttatcaactt aggaaatgat atgtcagttg
    58561 tacaaggatt gaactttcta ggcgcttctc tgccgattgc ttttacaggt ttgttttcag
    58621 gtatcgccca agggaaagtt gcagcagcag gtatccaaat tttagcgaaa aaacctgaac
    58681 atgctacaaa aggaatcatt tttgctgcga tggttgaaac atatgcgatt ttaggtttcg
    58741 ttatttcctt cctattagta ttgaatgcct aagcataaag gaggaaaagc agatgaacgc
    58801 cattgaaaaa atcatctctc agatgaatga agcagcagag caagaaagag cggcgttaga
    58861 gcaagaagag cgaatgaaga tcgatcaaaa ctttgaacaa aaacgtacgc aaatcgaaac
    58921 agaacatcaa aaacaaaaag aaaaacaaat cgaattactt gaaaaaaaat accgtcagct
    58981 taggaatcgc caacaagtag aagtacgaca agaaaatttg aatgcgaagc aggaattttt
    59041 acgacgattg tttgcagatg caatagctga aatggagaat tgggatgaat ctgaacaaat
    59101 ccagtttatc aaaaattcat tgtattcatt acctttgaca ggcaaagtaa cattcattgc
    59161 tggagaaaaa tcagcagctt atctttctca gacattgctg gatgaatgga acaatgaact
    59221 tccctttatg atggttttaa gtgatgaaac tgttgctgat caagcgggtt ttctgataaa
    59281 cgatcaagga gttcagtata attttctttt cagcagcctt gtacaagata tccaaggaac
    59341 gatgagtttt gagattgcca atcaattatt cgaataagga gggatcagtg tatggaatac
    59401 cacgaactaa atcccttgat tcgcggaaga gagcttgaac ttttgtccaa tgagacattt
    59461 gaacggatga ttcaaacaaa ttctgtcgaa gcacttggtg aaatattgaa atccacgatt
    59521 tacagtccat atatttatga cggatatgaa tatgacttcg aacagaatct tttaaaggag
    59581 cagagccgtc tatttcgctg gttaaaggaa tcagcaccgg agcctgaaat cgtttggatc
    59641 tatacgatgc gctatacttt ccataatctg aaggttttga caaaagccga gatgacaaat
    59701 caaaatctgg accatttgta tatcgacgat ggattttatt cattagaaac cttgaaggat
    59761 gccattcata cgcaagtttc aaatgaactc cctgaaagca ttatggacag tatacgagaa
    59821 gtacgtgatt attttgaaga gtctaccatt cttcagggaa ttgacgtgat ttatgatcga
    59881 cagtttttga cagagcagcg acggcttgcc gataagctag gatatcaaga attgctgaca
    59941 gaagtcattg cttttattga cttgaccaat atcacaacga tggctcgagg aatattgcag
    60001 aaaagaacat atgggttcat gacgacggta ctttccagct cgggaagtat cgaaaaagaa
    60061 gtatttttga actttgtaga taaagatatg gaagattaca cgaattttct attagagaca
    60121 gattatgctg attttctgcg ccctgtcatc cgtgagggaa atcttgatct cgtaaagctt
    60181 gaacagttga aagatgatta tctaagttca ttgtatcaag aagcacaaac ccaggctttc
    60241 ggaccactgc cactactagc ctttttgaac gcaaaagagg tagaaagcaa aaaccttcgt
    60301 ttactggtta tcggcaaaag aagccatttc gaaccagaaa caatcagaaa gcgggtgaga
    60361 caagtctatg acgcataaga tcggtgtagt tggcgacaaa gattctgttt tgccattcaa
    60421 attgtttggc tttgatgttc gttatggtac aaccaagcaa accgttcgtc aggcaattga
    60481 agaaatggca aagaatgagt atggcgtgat ttatgtcaca gagcaatgtg cagcattgag
    60541 cccagaaacg atcgaacgtt acaaggaaca aatgatacct gctatcgtat tgattcccaa
    60601 tcatcaagga tcattaggga tagggctgga agaaattcaa aaaaacgtag aaaaagcagt
    60661 aggtcaaaat atcttatagt atgaaaagga gatagaaatt ttgcaaattg gaaaaatcat
    60721 aaaagtctcc ggacctcttg taatggctga gaacatgtct gatgccagta tccaagacat
    60781 gtgtctagtg ggagatctag gagttatcgg agaaattatc gagatgcgtg gcgatgtggc
    60841 gtctattcaa gtatatgaag agacttctgg aattggtcca ggcgaaccgg ttcgctctac
    60901 tggtgaagct ttatctgttg aattaggacc agggatcatt tcacaaatgt ttgacggaat
    60961 ccaacggcca ttagatacct ttatggaaat cacacaaagt aatttcttag ggcgcggagt
    61021 tcaattgccc gcattggacc atgagaaaaa atggtggttc gaaccaacag tagaagccgg
    61081 agaaacggtg agtgctggag atgttatcgg gatcgtagaa gaaacaaaag ttataaaaca
    61141 taaaatcatg gtgccaaatg ggataaaagg aacgatcaaa acaatcgaaa aaggttcatt
    61201 cactattgat gaaacagtat gtgtcattga aaccgaaaat ggtgacaaaa atttgacgat
    61261 gcttcaaaaa tggccagtaa gacgtagtcg gccaatcaaa gaaaaattaa acccggatgc
    61321 acccatgatc actggacagc gagtgattga tacattcttc ccagtgacaa aaggaggagc
    61381 agcagctgta cctggtcctt ttggtgcagg gaaaacagtc gttcagcatc aaattgcaaa
    61441 atgggccgat gtggacatgg ttgtctatgt gggatgtggc gagcgtggaa atgagatgac
    61501 ggatgtttta aatgaattcc ctgaattgat tgaccctaat acaggagaat cgttgatgga
    61561 acgtacggta ttgattgcta atacatcaaa catgccagta gcagctcgag aagcttctat
    61621 ctatacggga atcacaattg cggaatattt ccgtgacatg ggttatgacg ttgctatcat
    61681 ggcagactcc acttcacgat gggcagaagc acttcgtgag atgagcggac gtttggaaga
    61741 aatgcctggt gatgaaggtt atccagctta tcttggttca cggcttgcgg aatactatga
    61801 acgatctgga agagtcattg cattaggatc agaaggtcgc gaaggaagta taaccgcaat
    61861 cagtgctgta tccccgtctg gaggggatat ttcggaacca gtcacacaaa atacattgcg
    61921 ggtcgtaaaa gtattctggg gtcttgattc cagtttagcc caaaaaagac attttccttc
    61981 gatcgattgg atacaaagct attctcttta ttcaacagaa gtagggaagt atatggatca
    62041 tatccttcaa gctgactggg catccatggt caccgagggc atgcgtattt tgcaggaaga
    62101 acagcagctg aatgaaatcg tgcgtttggt agggattgat tccctttcag ataatgaccg
    62161 tctgacatta gaagtggcaa aatccattcg tgaagattat ttgcagcaaa atgcttttga
    62221 tgatgtcgat acattcactt caagagagaa acaattcaaa atgttgaaga tgatcttgac
    62281 ctttggtgaa gaaggacgcc gtgcgttgtc gttaggtgct tatttaagtg agatcatgga
    62341 cggtaccgta tcgattcgtg aaaggatcgg acgaagcaaa tatatcccgg aaacagaatt
    62401 agaaaaacta gatcaaatta ttggtgaaat caagcaaacg atgcaaacga tcgtttcaga
    62461 aggaggcatg agcaatgatt aaagaatatc gtacgatcgg tgaagtcgtc ggtccattga
    62521 tggcggttga aaaagttgct ggtgtcaaat atgaagaatt aatcgaagtc cgtatgcaaa
    62581 atggcgagat tcgtcgtgga caagtattgg aagtccaaga agataaagca atggtccaaa
    62641 tattcgaagg aacaagtggt atcaatttac gtgactcttc tgtacgattt ttaggccatc
    62701 ctttagaact aggtgtatcg gaagacatga tcggtcgagt attcgatggt ttgggacgac
    62761 cgaaagataa cggaccagag atcctgcctg aaaagcattt ggatattaac ggagaagtaa
    62821 tcaatccagt agctcgggat tatccagatg agtttatcca aacaggtatc tctgcaatcg
    62881 atcatttgaa tacattagtt cgtgggcaaa aattaccagt attttctggc tctggtcttc
    62941 ctcataaaga attagctgct cagatcgcgc gacaagcaac ggtactcgat tcatcagatg
    63001 atttcgccgt agtctttgct gctattggaa tcacttttga agaagctgaa tttttcatgg
    63061 aagattttcg tcaaacgggc gcaatcgacc gttctgtcat gtttatgaat ttagcaaacg
    63121 accctgcaat cgaacgtatc gctactcctc gtatggcttt gactgctgca gaatatcttg
    63181 cttatgaaaa gggaatgcat gttttggtta tcatgacaga catgaccaac tatgcagagg
    63241 cattgcgaga gatttctgca gcacggcgag aagttccagg aagaagagga tatcctgggt
    63301 atctttacac gaacttagct actttattcg aacgagcagg aagaatccgc ggactaaaag
    63361 gatcggttac tcaaattccg attctaacaa tgccagaaga cgataaaact catccaatcc
    63421 ctgacttgac tggttatatt acagaaggac agatcatctt gactagagag ctttacaaga
    63481 gtgggattca gccaccaatt gatgtactcc cgtctctttc gcggttaaaa gataagggaa
    63541 ctggacccgg aaagacaaga gaagaccatg cagctacgat gaatcagctt tttgcagcct
    63601 atgcacaagg aaaacaggca aaagaattag ccgtagtttt aggtgaatct gctttatctg
    63661 atgtagacaa gatctatgcg aagtttgctg agcggtttga aaaagaatat gtcaatcaag
    63721 gattctacac gaaccgttcg attactgaaa cacttgatct agggtgggaa ctgctatcga
    63781 tgttgccaag aaccgaatta aaacgaatca aagacgacat gctggacaaa tatctgccag
    63841 aagggaagtg agttctgatg gcacgattga atgtgaatcc gacccgcatg gaattgactc
    63901 gattgaaaaa acagttgaca actgccacaa gagggcataa attattaaaa gataagcaag
    63961 atgaactgat gcgtcaattc atcctgttga ttcgaaagaa caaccagctg cgtcaagaaa
    64021 tggaagagtc tatgacagaa gcaatgtctg attttgtatt ggcaaatgct tcgatcacgg
    64081 aacctttcat cgaagaatta tttgttttgc cggcggaaaa cgttgatttg tctatcgtag
    64141 acaaaaatat catgagtgta aaagtacctg tgatgaattt tgaatatgat gaacagctga
    64201 cagacacacc tttggaatac gggtatttga attccagtgc cgaactggat cggtcaatcg
    64261 atcggtttac taccctgctt ccaaaacttt tggaactgac agaaatcgaa aaaacttgtc
    64321 aactgatggc gagtgaaatc gaaaagacac gtcggcgagt caatgcatta gaatatatga
    64381 cgatccctca attagaagaa acgatctatt atattcgtat gaagctggaa gaaaacgagc
    64441 gcgcagaagt aacaaggttg atcaaagtaa aaaatatggg taccactgac taagtggtat
    64501 ctttttttaa aagaggaaaa cgatgattaa acaaagcaaa caaataaaga aacgtttgcc
    64561 ggctgttcag cttctcgcat taggattttt tctcctgatt ttagttggcg gcggcctact
    64621 taccctacca ttttttagta atagtgggca tgggacaaat tttatcgatg cgcttttcac
    64681 tgcaacttct gctgtctgtg taacgggatt gacaacattg aatacagcag agcattggaa
    64741 cagtgctggt caatttttga tcatgctttt gattgagatc ggcggtcttg gttttatgat
    64801 ggttccgatt ctatgctttg cattgataaa gaagaaagtc agtctcagca cacgtattat
    64861 attaaaagaa gcgctgaatc tagaagaagt atcaggtgtg atgaagctca tgctttacat
    64921 cctaaaattg gcctttgtta tccaattgat tggtacggtt gctttatctt ttgtatttgt
    64981 gcctgaattt ggctggttga aaggtatctg gtacagtttg ttccatgcaa tctctagttt
    65041 ttgtaatgca gggtttgatt tgttaggaga cagtttagcc aatcaccagc aaaatgttta
    65101 tttgatcatg gtggtatctg ctttgatcat ttctggtggg ttaggcttca tcgtatggcg
    65161 tgatttactg gaatatcatc agaaaaagaa gatgacgatt cattcaaaaa tcgcattaac
    65221 cgttacaggt acccttttat taggcggatt cgttgtgttc tttttcacag agcgcaatgg
    65281 ggcaacgcta gtggaaggga atatgtttca acgattggca aacacctttt ttatgagtgt
    65341 gacacctaga acggctggat attattcagt tgattacctc catatgagcc atgcaggtct
    65401 gattttaacg atgtttttga tgtacatcgg cggaacatct gggtcgacgg ccggcggatt
    65461 gaaaacaact acgttaggtg tgttgctgat ccagatgcgt tcgatgttga aaggacgtac
    65521 gagagcggaa gcatttggcc gtaccatccg acaaggcgca gtgttgcggg cacttacttt
    65581 attttttgtt acgttgacgt tgtgtgtagt cgccatcatg attttatcgg tgactgaaac
    65641 gatacctaaa acttctggca tcgaatatat tgcttttgag gtcttctctg cgtttggtac
    65701 agtaggtctt acgatgggat tgacgccaga cttgactgca atagggaaat tgatcattat
    65761 agcgcttatg tacattggac gagttggtgt attgactgtg gtcttctctc tgatgacaaa
    65821 agcacaaaga acacaatcaa aatacaaata tccagaagaa agtgtcatga ttggataatt
    65881 tcatcttctg acagaaacct agtgatttag taaaataaga aaataaaata atttttcttg
    65941 cttcttggat actttaagtg caaaatgttc gtttattttt tgaggcacta cccgttgttg
    66001 aacctcgggt agtgcctctt ttttattgaa aggaatcaat aagatttgat tcctttattt
    66061 atcaataggt tataattcat atttttttat tttttctttt acgatactta gtccagaagc
    66121 taaaatttca aggttattag aggataggga aattcgaacg aatgcatctg gttgcgcacg
    66181 tgtagtaaag cgtctggaat gataaagtcg gataccgtta gcaagaaagt cattctcaat
    66241 tatcttaggg tcaatctcag gatcaactgg aagaattcga tagtaaggga cacgattcgt
    66301 tggtttttct actttgaaaa gagaatcaaa aagctgattg gctctttttg taagacttaa
    66361 tttagtttct aagatcctat ttgcttcatt tgaatggagg atttgagtga tgacttccac
    66421 atctaatcca gaagttttca catttatatt aaaaattgct tgttctatct tgctttggac
    66481 atgtactgga actacaagat aagcaactcg caatccagaa caaataaatt ttgtcattcc
    66541 agctaagtag attgtttggt taggtaataa attttggaaa ggaggaatga tcgtatgttg
    66601 atagtaagtt gttaaaaatg aatggatatc atcttcaata acccatagac cttctttttc
    66661 aatcacttcg gctaattgct ctcgtctttt gtgagagatt tgaaacccta taggattatt
    66721 acaagaaggc ataagaaaga caccatgaat cttctttttt ctacattcct gaatcaatat
    66781 atcaggtcgc attccttgct cgtcaaaatc aattggaacg atctctaaat gatataactg
    66841 agccaattca atgaaattag cgtatgtata tcggtctgtt gctattctat cacctggaga
    66901 gaaaagtgca gaaagtgtga tagccaatcc attttgaaca ccggaaacaa tagatagaga
    66961 ttctaaggac ggaatgactc cttgagtttt taaccaattt gctgcagtag tcaattgaaa
    67021 atttgtgcct ttgggatctt ggtaattaag caaaccaaca agatttgggt tattactagt
    67081 tgattgaata aacgggacaa cgaatttatt acattcttca aaagaactaa ccagaccaaa
    67141 atcaatgata tgttcggtta cttcattggt agagatggtg atggactcaa agacattggg
    67201 agaattaaat gtgccacttc caatattcgt atacagcaaa cctttctcaa ttccatattt
    67261 ataagccttg ccaacggtag taaaatttaa atctaaataa tcagctagct ctctttggga
    67321 gggtaactga gtgtttttct gtaatttatt ctttaaaata tcttgttcta attgttgaat
    67381 caacgatcga taataaggac gcttcaattt ttgtttgtct ggtcgccaag ttaacgggta
    67441 attgtcataa gaattaatag gcattttctc cactccttaa aattgtatac catacaattt
    67501 taccattgat tgtattgatt ttagaagtta atcttttgtg aggagggaat tgtctatgaa
    67561 aattattaat tttgaggaag tagtagcttc ttttagtttt gaagaagcga ttcaagtaat
    67621 gaaaaaatgt ttttttgatt atgaaagtgg taaaatcagc caaaatcctc gattggtgga
    67681 aattttacct gacggaaaag gcaaaaatat gtttgcgatg atgcctgctt atttggggga
    67741 gaacagatat tttggcacaa aagtcattac cgcttttccg gataatcatc agtatcattt
    67801 accttctcat ttaggagaga ttttcttgtt tgatgctcaa aacggactac ccaaagcatt
    67861 gataaacgca aatgcagtca cctggattcg aacagctgct gtatctgcgc tggcaacaga
    67921 ttacttagca aatccgcatg cagaaacact tgctttaatt ggagctggtc aacaagcatt
    67981 gtcgcatttg actgcgattt ctaccattcg tcccatcact acagtgtttg tttacgatat
    68041 tttagaagaa agacgaaata cttttatcgc taaagctaaa aaacagtatc ctaaaataac
    68101 gtttataaat gcaatatcag tagaagatgc agtacgtcca gcggaaatca tttgtacgtt
    68161 gacctctagt aaagaggcat ttcttcaaaa agagtgggta agggaagacg cacatatcaa
    68221 tgctgtgggc acatttactc ctgatacaag agagataagt acatcgttga tggaaacttc
    68281 tcaaatctat gtagatgatt actcagctgc cttaacagaa agcggggacc tgcttatagc
    68341 catggcagaa ggagatttag aggaatcttg tattgttggt acattaggcg aattagtttc
    68401 tgagaaaaaa acattttata aaggagaatc gaggccgacg attttcgacg cagttggtct
    68461 agctattgaa gatctttgtt gtgcagaata tatttataac aaattagaga aaggggcttt
    68521 aaaatgaact tagattatta cgtagttgat gcatttgcag atgaggtgtt caaaggaaat
    68581 ccagcagcgg tttatgtgct agaagaatgg ctgccagaag gtacgatgca aaaaattgcc
    68641 attgaaaaca atttatctga aacagcattt acagtgaaga aaaaccaaga atttgagctt
    68701 agatggttta cgccagatcg agaaatcgat ctttgtggtc acgcgacatt agcaacagca
    68761 tttgttttat ttaactatta caaaatacct gatgaaacga ttaaattttc tactcaaagc
    68821 ggtaatctat ttgtgacaag aattaaagat tattattaca tggattttcc tagtattatg
    68881 ccgaaaaaag tgccgatatt gtctgaatat gaagaagcga ttggggctaa gattaaagaa
    68941 gcctacttag cacgtgattt atttttcgta ttagaagatg aaaaaacggt ggctaaattg
    69001 cagccagatt ttacagctat caaaaatttt gaattaggaa taggagtcat cgtgacagca
    69061 gaaagtgtgc aaaaagactt tgtttctaga accttctttc caaaattaac aatcaatgaa
    69121 gatccagtat gtggatcagc acattctaat cttattcctt attgggcaga aagaatgaat
    69181 aaaacaaaat tgtcagctta tcaaatgtca tcaaggggcg gggatctaga atgtgaatta
    69241 aaagaagatc gtgtgataat aggaggaaaa gcagttttgt tttctaaagg tacctcgttt
    69301 atttgatttc tttcaaatga attaaaaata aattggtgga gagtggcaac cttatagcta
    69361 caatggttgt taaaaaaaga cgcaaacttt ttcaatttaa tgaaaagttt gcgtctcatt
    69421 ttaattattg gtgtttgttt tttattcaat caagagtaaa atgccccata ctaactatcc
    69481 tcggatgact tcgattttat aaccatcagg gtcaatgaca aagtagtaag aaggcgcagt
    69541 gccaggtaat ccttttaagt cagtaatctc aagaccggca gctttatgtt gctcgtgtaa
    69601 tttttctaga tcatcagctg ctatagcgat atgaccatag ccgtctccta gatcatacgc
    69661 accatgatca tagttgtaag tcaactctaa ttcatagtca tccccaggaa gtgtcaaata
    69721 aacaagtgta aatttgtttt ctggaaaatc acggcggcgg ctttcttcaa aaccaaatgc
    69781 cttttgataa aattcgattg atgcttcaag atctttcact cgaacacatg tgtgagccat
    69841 tttcatagat tcatctctcc ttttcctttt actcaatcat aacaaaaaga agtaaggaaa
    69901 tagttgattt gtgcttgttc atgagaaatt tctcatgtaa aatggataga aaataaaact
    69961 gttaaggtgg aaactattca tggataaacc aatttttggt gaaaaaaagc aatttgctga
    70021 ttatcaaacc cgttatgcag catatattgt catagaaaga gatcagcaga ttgcagtaat
    70081 cgaggctcca aatggtgctt tttttcttcc tggcggggaa attgaaggag aagagacgaa
    70141 agaagaagcg attgtccgtg agctgataga agaaatggga attgtggcag aaatttcttc
    70201 ttatctggga gaagctgatg agtattttta ctcgaattat cgtgcaactt attattataa
    70261 tccaggatat ttctttgttg ctgattcttg gaagcgtata ggagaaccaa ctgaaaagac
    70321 caataaaatt tggtgggtga gccctcaaga agcgcttgtg aaattgaaac gtggtagcca
    70381 ccgatgggca gtcgagagat ggatcgaaaa aaataaaaac tgaaagaaag cgagtagatc
    70441 agctttctct cagttttttt atttattaca gtacatattt tttgattgct tcagctacac
    70501 cgtgctcgtc gtttgatgaa gtgatgacat ttgctgcttc tttgacaaga gaaacggcat
    70561 ttcccattgc tactcctaga ccagcatatt caatcatgga taagtcgttc tcgttatctc
    70621 cgatagccat gatttcatct tgatcaatac caaggtgctg tgcgagattc gcaactgccg
    70681 cacctttact tgcttcttta tttagtactt cgaagtaata gggcgcactt ttgaccgttg
    70741 tgtatttatc acggaaagtt tgcggtaaac gcgcaattgc tgcatctaaa atttctggtt
    70801 cgtcgatcat catcattttg acgatgttca tgtctggtgt catttcttca ggtgttcgat
    70861 attttagcgg catcttgact aaacttgctt cgtggatcgt ataagcgctg atatctcgat
    70921 taggcgtgta gatcgctttg tcatcgatcg aatggagatg actgccgaca cggcgtgcca
    70981 tcacttcgat ctctaggaaa tcttcatgtg tcagtccgtg acgggcgatg attttacctg
    71041 atttcgtttg ttgtacgagt gccccgttat aagtaataac gtaatcatta tcttgaaaaa
    71101 ggttcaactc gtttaactgt tcctggacac ctggtaaggg acggccagtg cataaaacga
    71161 tcgaaacacc ttttgcaaca gcagcggcaa tcgcttcttt tacttctttt gttacttctc
    71221 ttttctcatt taataaagta ccatcgatgt cgatagcgac tagtttgata gacataattt
    71281 cctccaatat attttaattt atttcaatca atgcgccatt acggatatgg ctggcaaatt
    71341 cttgataagt ttcgtcaaat aaatcataat gatcacgcaa ggacggatca accatttctt
    71401 ttggaaagaa aaatcgctca tctccttgtt cttgtcccgc taatgcagca acaagctgac
    71461 taacctgtga taattctatc aaagttccgt catttcgcaa aatctcgatt tgtgtgcgat
    71521 gtctgccttg ttctgggcga taaaaatcat aaggtagatc gtagctagaa ttcacagcag
    71581 tgtaataaac aggattatat ccgacctttt ctactaacaa agtgagttct tggactaatt
    71641 ttgaatcccg gttgccagaa aaagtagcag atttcagtgg tttacgattc aaaaaacgtt
    71701 tagctaaatc atttaaaata ggatcagcag attcagacca ttgagtaaag taagtgccca
    71761 agaccccatc atcaagtttg aggtattctt ctaaagtaaa gcttccgtca agaaatggca
    71821 gaagtaattg agaatggagt tcaaaactgc tggcatcttc ttgatacaac tcgtgtgcgc
    71881 gatgaagcag atgctccaaa attacttcca taccacggga aacaggatgg aagtacactt
    71941 gcacatacat ttgatatcgg ctaacgatat aatcttccac tgcatgcatg ccactcatgg
    72001 aaaaagcgat cccgccttca taaggacgaa tcacacgtaa tattcgggta agatcaaatg
    72061 ttccatattc tgttccagtg aaataagcat cccgaagcaa atagtccatt cgatctgcat
    72121 cgatctgact agaaatcatt tggacaactt gtgggttggg gtaagttttt tggatcacac
    72181 tagctacctt ttcaggaaaa ccttcttcta ctcggttaag aatctgatac acttctgttt
    72241 ctggagaagt gatgatctct acagtgatgg cttcatgatt cgtatggaag atatgttcaa
    72301 atgtatgaga gtaaggacca tgaccgacat catgcaacag tgccgcacat aaagtcacca
    72361 gccgttcctg atcatcccag cctccgttgc ctattttttc ttttgaaaaa tttcgagaaa
    72421 agatatcgca gattcgtctt gaaatctcat aaacgcctaa tgaatgagta aaacggctat
    72481 gttctgctcc gtgaaaagtg aaagaagaag tgcctaattg tttaatgcgt cgtagacgtt
    72541 ggacttcttt cgaattgatc aaatctaaga ttacttgatg ctggacatgg atatagttat
    72601 ggactggatc acgaaaaact ttttccatag gcagcatttg atatttataa ggggttgtca
    72661 taatgtagct cctttatgtt attacgttgt tccattctct ggatttggtt gttccattgc
    72721 tccagctgga tttctttttt tagccagtcg gtagaatcct gtaaatcact tgcagaatac
    72781 agcttttcaa aagcttttgc aaatgcttct ttgacttctt ttacaaaaag gggttggtca
    72841 agcagttctt caagtgttgc cattgtagca ggatccactg caggatagcc atttgtacca
    72901 aagtcttctt ttaaagcaga ttgataaaat cgcttcatga cttgcccgcg tttttcctga
    72961 ttaccgttga cacttaagta catcataaca gaaatcccat ttttgatccg acgctgagcg
    73021 ataccggcaa attttttgcc atggatgctt aaatcataag ttccaggaca ataagaatgg
    73081 gtgatttcga atgcttgtat cggtgccttt ggaaaagcgt atttagtcaa taggagcatt
    73141 tgctcgtaag cgtcatctat ggttattttt tttgtgtcac tttgggggaa aatcaaagaa
    73201 atattcaaaa ttccttcatc tgatacgatc cctaatccac ctgaatttct gatcactgga
    73261 gcgtaacctg catctttcag tgtttggatc ccgtcactta gaaaaggagt tcgaacatcc
    73321 ttcataccta agatgactgt gcagtcatat tgccagaaat gtaaaaagat ttgctggtgt
    73381 tctttagaat attccgttaa tatatctgtt aatgcaaaag gggaaaagtc gtttttttga
    73441 taaatgtgct gatctagtcg aatcgcaggg ttcattcaac cggctccttt gtataaattc
    73501 atattttcta caatattata ctctaaaatc aaagagttgt tttggaaaaa aggttttctg
    73561 taaaaatgga caagttggtt gacaaaacaa aagaaactag gaacaatgta gctatgagaa
    73621 gtaaaggaga atgaataatg gaattatcac aaggaacacc gatttcaatc aaattgcgta
    73681 caaaagtaaa acagcaaggg gaagtgcaag atttttattt tgatttgaaa gggcaaatgg
    73741 tgacaattgg cgatacgctg tacatacgtt ataaagagat tcaggaggag acgggcgatg
    73801 agattcctgt aacgatcaaa ttagttccag atggtcatgt acagctgatc cgtgcaggag
    73861 aaatgcggat gcgtttgaaa tttggctata aagaacggtt agaaacatcc tatcgaacac
    73921 catacggtat gatccagatt gctactttta cgaaagaact gcatgttagt ttgaaagacc
    73981 gtcccacagg agggaaggtt cgaatcgatt atgatctgtt catgggaccg gaaaaaatcg
    74041 gcgaatatta tttgaccttg gattttacag cgtaaatata aaagaaaagt ttgatttttt
    74101 gtgtctgatt ttgtatgata aggagtagac tgtgaaagga cgtgtcctga ttggaaatta
    74161 gtgtatttga aggtgcaaac aaaagcgaat tatcaatgat cgaagtagca cacgcgatct
    74221 tagaacaacg tggcgatgtc atggattttt ctgatttagc taaccagatc caaaactatc
    74281 ttgggaaatc agacagtgaa attcgtgatt cactcgcaca attttatact gacttgaata
    74341 ttgacggcag ctttatctca ttaggggata atcgttgggg cttgcgttca tggtatgcta
    74401 tcgattcgat cgatgaagaa gtaaaccatg gaatggacga agaggatgaa gatactccac
    74461 gtcgccgtaa acgcaagaaa gtcaacgcct ttatcaatga tgatgaagac gcgatcgatt
    74521 acaatgacga tgacccagaa gatacagatc tgacagaaga agatgacgat gatctgtttg
    74581 atgacgatga tgatgaagat gaagaaatcg ctgcttataa ttctgacttg caagaaatcg
    74641 gagcagatga cccgacagat gatgaagaag acttgccagg aaatatcgaa gaagatttaa
    74701 gtatcataga cgaagacgat gatgatgaag attatgaaga ggaagaattt tctgactctg
    74761 aaaaagaata aacattattt tcttacgaga ggttgtgaca gagacgatca gcctatcaga
    74821 ataaggaggt gtgacacgat ttttgtcaca ccttcttttt tgtatataaa aatcccaagt
    74881 aaatacataa aaaataaaaa aattgaaact tttagcgcac cttgtacgtc atattaagtg
    74941 aggcagaaag gagtggagaa tgatttttac tggatataag aaaaaacggc agatcgaaaa
    75001 ataccgtcac ctgctgaatg gactcatcga aactgattat caaaaaatgt tccgtatcac
    75061 gatgaattac gtacatgata aagaagaggc cttagatgtg atgcaagata gttttcataa
    75121 agccctgaac tattttgaaa aagaaaaaga gatcgagcat tttagtgcat ggttttatcg
    75181 gatattgatc cgcacggcat tagatagttg gcgcagacaa aaaaggacgc ctgtggactt
    75241 atttgaaatt caagatatcc cccaacagtt acctttagaa acttccgttg cagagctcca
    75301 tatgatttta gataaaatag acagtccgga aaaagagatt ctgatcctct acttttttga
    75361 aggatttcgt ttgaaagaaa ttgcgactat cttggatatg aatgaaaaca cagttaaaac
    75421 gaaaatgtac cgctcattga atgctttacg tcaaatccta gaatgaagga gagaagagtg
    75481 ccatgtattc ccaaaaagaa aatttaaaaa aactaaagaa aacttataaa gaacaagcaa
    75541 ttcccaaagg gcttaaagag gagatccgcc agcgttttat tatcgaagag caatcattta
    75601 taaaagcaaa gcggagaaag agacgtctcc aagggttcgg agtatctttg gctatcgtaa
    75661 tgattacaac aagtagcctt ctttttaata gtcaagttcg aagctttgca gaagatttac
    75721 ccatcttagg atcagtcatt gaattgatct taggcgaacg ctttactgat cgatctgaaa
    75781 aaatcgatat tcaggtaccg aagatcaata cacaaaatga gaaagaaaat aaaacaattc
    75841 atggattaaa tcaaaaatac tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag
    75901 aatatggaaa ttttgaaacg gatcattacc aagttttggg agattatcag aaaatcgtag
    75961 acgataaccg gtttctcgtg attgaaaggc agatcaccca aacagcagca gatagccatg
    76021 ttgaaaaaag atacgacaca atcgacaaga aaaacagtgt ccagctctca cttccacttt
    76081 tattcaaaga tgacacctat ctatctgttt tgacaaaaga agtcaaacgg cagatggccg
    76141 aacaagtcaa ggaagatcca tctaagtact attggactga gcaagatatt caagaaggga
    76201 cgatagaaaa accaacactt gttacgccta cacgaagttt ttatttgaac aaagagcatc
    76261 aattagtttt gactttttct caatacgaaa ttgctcctgg ttatatgggt acaccagaat
    76321 ttgtcattcc taagtccgtg acaaaaacga ttttggcatc ggaagattat ttggatcatt
    76381 agacgagaag tagttgcgac tagcagtttc aattttcaag taagtacaag taaataataa
    76441 gaagagaaaa agagaaagaa gagatatagt gaaaagaaat tatttagtta tcagtattat
    76501 tgctttatcc gtgcttttgt ttttcgaaat tacttcggtg gcctccttga tcaccgaagt
    76561 aaaaggacaa acacactctt cggagcattc gtcacaatct ttcaaacaat cagaacaatc
    76621 aaccattaaa agccaatcaa ctgaaacagc taacgtcaaa gaagccattc aagaatcagc
    76681 ggaaaaggta caaagtcagt catcacaatc aacacaagac agtacaaaaa cgagccgaga
    76741 tccggctgtc aaaagcgtcg caatcagctt tgatgatgga ccaggagcga ccacaacacc
    76801 gcagcttctg cgaatcttaa aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga
    76861 aaatacggcg cagcatcctg aaatcgtgaa gcagacagct gaagcagggc acgagattgg
    76921 caatcacaca tacgatcatc agaatctggc cactctttct gcccagtcga tgacggagga
    76981 agtgacgaaa gcagacactg agatcaaaaa agtcactggc aaaaccccca cttttgttcg
    77041 tccgccctat ggttctgtca cgaatgtagg agcaacgatc atccagcgac cgatcatcga
    77101 atggtctgtt gattcagaag attggaagac aagaaatcct gatttgattt tgcagaagat
    77161 ccaagcaaca gtatacgatg gggcaattat tttattccat gatatctatc ctgaaacgat
    77221 tcgtgccgtt cctcaagtga tcgattatct gaaagagcaa gggtatcgca tcacgacagt
    77281 tggggattta ttagggcatc caacggctgt cgagaactat tatggcagaa acgatcatcg
    77341 gccagttcag taagtgagac aagtatcttt aataaataac acattttaat aagtagttga
    77401 acacataact accttacttg caaaggctta acaatatcta tacaggtaca acaaaaaact
    77461 gtccaccaat tttctttggc ggacagtttt cacttaaaaa taaagaagca actattcgta
    77521 aattatgcta aaatagcagt agaacgcgtc ctgagggaat cgaacccccg tctcaagaac
    77581 cggaatctta cgtgatatcc actacactaa ggacgcaagc actgggataa ttgtaactaa
    77641 acagataaaa aattacaagg ggattggaca aaaaaatgaa atttgagcaa aatttttcac
    77701 aaaaacagca gcagacacag aaactagcca tgacgcaaca gttgcaacag tccatccaag
    77761 ttttacaata taacagtgag gagctgttgg cattcgttga gaatcaagca atggaaaatc
    77821 cattggtgga agttgttgag ccggaatggc agccagatta cataaaagct tcttcatctt
    77881 cttatgaagg agaagagaca aattacctaa atcagattcc tgatacaaaa gggtctttat
    77941 ttgattcatt gatcgaacaa gtccatttga attaccgaga tacttttttg aggaaaatcg
    78001 ttctttatct tgtagaatac atcgatttga acggattttt gacgattagt ttagaagaag
    78061 ccggaaaaga gattggagct acgcctatcc aaatgctcga tgcgttgact ctgattcagc
    78121 aattagaacc agctggagta ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag
    78181 aacgagatga ttatgcgccg gaattagctt atatcgtgtt ggaagaatgt tttgaggaac
    78241 tggtggagcg aaaatggaaa gaaatcgcac aacgtttttc tgttgatcta catgctgttc
    78301 agcagatatt cgactattta caaacgttga gtccaagccc tggccggatt ttcgaccgta
    78361 gcagcgaatt gtacattatt cctgatgtcc gggtattagt ggacgacgat aaaaatgtac
    78421 aagttatctc aaaccgaaaa aatcagccaa atatacgctt tcaagagagt tacttcaaac
    78481 aaatgtcaca acaagcagat aaagaaactg aaaattattt aaaagaacgg aaacaggaat
    78541 tcgaatggtt gaagaaaacg atcctacaac gtggggatac aattttgcgg gttgctcaag
    78601 tcattgtttc ccggcaaaaa gctttcttta tcgacaagga acgtccaatc aagcctctta
    78661 cgttaaagga agttgcaact gagatagatg tgcatgaatc cacggtcagt cgtgcagtca
    78721 acgggaaata tttggaaaca gacttcggtg tatttgaatt aaaaaaattt ttcactacac
    78781 gaattcctac gaatagtaca gaacagacag aagacctttc agcagataca gcgaagaaaa
    78841 agctgcagga attagtcgat caagaagaca aaaacaagcc gttgtctgac cagaaattgg
    78901 ttgaattgct gaaaaaagat gagatagcga tctcaagacg aacagtagca aaatatcgtg
    78961 atttattagg aatcccgagt tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa
    79021 gactagaaaa agtatttcac tttttctagt ctttttagtt gaaaaacgag agcaaaactg
    79081 ttagacttac agtcgtgagg ttgagatttc cctttattga tgtgaaattc gaatagttaa
    79141 tttctttttt tatcgtatat gggtcgtttt aagtctacat tggacgtaaa gtgtccaact
    79201 aaggagatcg ctatgttgga tgaaataaaa atgattgaat ctgttgcacc agatattatc
    79261 gaagtgttgc aagaacgcta taaaatttta cgaaatatct attggatgca gccgattgga
    79321 cgtcgaagtt tatcggaaag tatggggatc acagaacgag tattgcgaac agaaacagat
    79381 cttttgaaaa atctgaattt gatcgacacc tcaaaaagcg gtatgacgct gacaactaag
    79441 ggcgaagaag tttatcaaag ccttgaaaac ttcatggatc agcttttagg tacgcatcaa
    79501 accgaacaac agttggcaga atattttggt aaccaacgct gtatcgttgt ttcaggaaac
    79561 agcgaagagc agaccaaagt ggctgatgca ttcggcgaag cgctgagcga agccctagac
    79621 cgactacttc cagaaggcga gaatatcatc gctgtgatgg gaggaactac tatggctgtg
    79681 gtagccgaac aattgtctaa tctggaaaat aagaaacggc ataacctgtt tgtccctgca
    79741 agaggcggga ttggtgaagc gataactgta caggcaaatt ctgtcagtgc aagaatggct
    79801 gctaaagcaa acggtaacca ccgtgcactt tatgttccgg aacaattaag tctggcaact
    79861 tacaattctt tactcaatga gccatctatc caagaagtct taaacttgat tagtgaagcg
    79921 aattgtgtta ttcatagtat tgggcgtgca ttacacatgg cagcacgtcg taagatgact
    79981 gaaaaagaat tagtcatgct gaaacaagca aatgctgttg cggaatcttt tggctacttt
    80041 ttcaatgaaa aaggtgaagt cgtttataag gtccctagaa tcggcataga attgcaagat
    80101 ttggaaaaag tcccgatcat catggcaatc gcgggcggaa aatcaaaagc caaagcaatt
    80161 cgagcttata tgaaaaatgc accaaaacaa acgtggctca tcactgatga agctgctgca
    80221 aatgagattt taaaaggggt aaccctttaa aataaatatt tttttgattt tcataaggag
    80281 gaaatcttaa atgacagtta aagtaggtat taatggtttt ggacgtattg gacgtctagc
    80341 attccgtcgt atccaagatg tagatggaat cgaagtagta gcaatcaacg acttgacaga
    80401 tgctaaaatg ttggcacact tgttaaaata cgacacaact caaggacgtt tcaacggaac
    80461 agttgaagtt catgaaggtt cattcaacgt aaacggaaaa gaagttaaag tattagctaa
    80521 ccgcaaccca gaagaattac catggggcga actaggcgta gatatcgttc tagaatgtac
    80581 tggtttcttc acttctaaat cagcagctga aaaacattta acagctggtg ctaaacgcgt
    80641 tgttatctct gctcctggcg gaaacgatgt tccaacaatc gtttataaca caaaccacga
    80701 aacattaact ggtaaagaaa cagttatctc aggtgcttca tgtactacta actgtttagc
    80761 tcctatggct aaaacattga acgacaaatt tggtgttgtt gaaggattaa tgacaactat
    80821 ccacgcttac acaggtgacc aaatgactct agacggacct catcctaaag gtgacttccg
    80881 ccgtgcacgc gctgctgctg caaacatcgt tcctaactca acaggtgctg ctaaagctat
    80941 cggtttagta attcctgaat tgaacggtaa attagacgga gctgctcaac gtgttcctgt
    81001 accaacaggt tcattaacag aattagtaac agttcttgaa aaagaagtaa ctgttgacga
    81061 aatcaatgca gcaatgaaag aagcttcaaa cgaatcttac ggatacaaca cagacgaaat
    81121 cgtttcttca gatatcgttg gtatgactta cggttcatta tttgatgcta cacaaactaa
    81181 agtaatgaca gttggcgaca aacaattagt taaaactgtt gcttggtacg acaacgaaat
    81241 gtcatacact gcacaattag tacgtacttt agaatacttc gctaacttat aagatagaca
    81301 aacgtacaag tgtaaattat agacatgtac atttaaaagc ggggaagcaa tcgcgcttct
    81361 tccgcttttt ttgataataa gtttattcag gaggaaataa aatggctaag aaaacagtga
    81421 aagatatcga tttaaaagac aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga
    81481 aagacggcgt gatcactgac gacactcgta tcaaagcagc attgccaaca atcaactacg
    81541 ttctagaaca aggcggaaaa gcaatccttt tctctcacct tggacgtgta aaaacagaag
    81601 aagataaaga aggaaaatct ttagctccag ttgcaaaacg tctaggcgaa ttattaggta
    81661 aagaagtaac attcgtacct gaaactcgcg gagaacaatt agaagaagca atccgcaaca
    81721 tgaatgacgg cgacgttgtc gtattcgaaa atacacgatt tgaagatatt gacggtaaaa
    81781 aagaaagcaa aaatgataca gaactaggta aatactgggc ttcattaggc gatgtatttg
    81841 ttaacgatgc tttcggtaca gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa
    81901 ctggtatccc aacagttgct ggattcttga tggaaaaaga aatcaagttc atcggtgaag
    81961 cagtagaaga accaaaacga ccaatgattg caatccttgg tggcgcaaaa gtttctgata
    82021 aaatcggcgt aatcgaaaac ttgattccta aagcagataa aatcttgatc ggtggcggaa
    82081 tgacttacac attctacgaa gcaaaaggaa tcaagatcgg taactctcta gtagaagctg
    82141 ataaagtgga actagcaaaa gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa
    82201 tcgacaatgt ctgtgcacca gaattttcaa atgatgttga aacacaagta atcgaaggag
    82261 acattcctga tggtttgatg gctttagaca tcggcccagc atcagtaaaa ttatttgctg
    82321 atacattaca aggtgcaaaa acagttgttt ggaacggacc tatgggtgta tttgaaatga
    82381 gtaactttgc taaaggtaca atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa
    82441 caacaatcat cggtggcgga gactcagccg cagcagcaga acaattagga tttgctgaca
    82501 aattcacaca catctcaaca ggtggcggcg caagcttgga attgcttgaa ggtaaagaat
    82561 taccaggact agcagcaatc aacgataaat aaacgaccaa gtttgctcaa ttctgacgag
    82621 taatcgatca aaaggtgaag ggagttttca tgactcccta gccatttttc aataaattaa
    82681 ggagtgtgtt cctcaatgcg taaacctatt atcgcaggta actggaaaat gaacaaaact
    82741 gcatcagaag caaaagaatt tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa
    82801 gttgattcag tcatcggatc tcctgcatta ttcttacaag aattaatgac atcagctgaa
    82861 ggtacagaac taaaaatctc agcacaaaac tgctactggg aaaactcagg tgcgttcact
    82921 ggtgaaactt caccagcagc tcttgctgat ctaggtgttc attatgtgat catcggacat
    82981 tcagaacgtc gtgaatattt ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct
    83041 atctttgcaa atggtatgac accaatccta tgctgtggcg aatctttaga aacctatgaa
    83101 gcaggcaaaa ctgctgaatg gatcgaagga caaattacag ctggtttagc tgatctttca
    83161 gctgaacaag tgtcaaacat ggttattgct tatgaaccaa tctgggcaat cggtactggt
    83221 aaatcagctg acgcacaaat cgctgacgaa atttgtggag ttgtacgtca aacagttgaa
    83281 aaactttatg gaaaagaagt atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa
    83341 ccagaaaata ttgctgaata catggcaaaa gaaaatgttg acggtgcttt agtcggtggc
    83401 gcaagcttgc aagcagattc attcttgtca ttgttagacg ctgtaaaata attgttgaaa
    83461 atttgcgaaa aatgcaagaa atgtttgcac taatgagcaa aattaactaa aatcgtatat
    83521 gagggaaatc ccttaggaaa aaacaaactc aaaggagaga caaaacatgt caattattac
    83581 tgatgtttac gctcgcgaga tcttagactc acgtggtaac ccaacaatcg aagtagaagt
    83641 atacacagaa agcggagctt ttggccgcgg aatggttcct tcaggtgctt caactggtga
    83701 atacgaagca gttgaattac gtgacggaga caaagctcgt tatggcggta aaggtgtaac
    83761 taaagctgtc gacaacgtaa acaacatcat tgctgaagca atcattggct acgatgttcg
    83821 tgaccaaatg gctatcgaca aagctatgat cgctttagac gggactccta acaaaggtaa
    83881 attaggtgcg aacgctattt taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta
    83941 cctagaagta cctttatacc actacctagg cggattcaac acaaaagtat tgccaacacc
    84001 aatgatgaac atcatcaacg gcggatcaca tgctgataac tcaatcgact tccaagaatt
    84061 catgatcatg cctgttggcg ctccaacatt caaagaagct ctacgtatgg gtgctgaagt
    84121 attccacgca ttagcttcaa tcttgaaagc tcgcggatta gcaacttctg ttggtgacga
    84181 aggtggattt gctccaaacc ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc
    84241 aatcgaaaaa gctggctatg tacctggtaa agacgttgtt cttgctatgg atgctgcttc
    84301 ttcagaattc tacgacaaag aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga
    84361 aaaaacaact gacgaaatga tcaaattcta cgaagaatta gtttctaaat atccaatcat
    84421 ctcaatcgaa gacggattag acgaaaacga ctgggacgga ttcaagaaat taactgacgt
    84481 attaggcgac aaagttcaat tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt
    84541 atctgaaggt atcgaaaaag gaattgctaa ctcaatcctt atcaaagtga accaaatcgg
    84601 tacattaaca gaaactttcg aagctatcga aatggctaaa gaagctggct acacagcagt
    84661 tgtatctcac cgttctggtg aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac
    84721 aaacgctggc caaatcaaaa ctggttctct atcacgtact gaccgtattg ctaaatacaa
    84781 ccaattacta cgtatcgaag accaacttgg tgaagttgca gaatacaaag gtttgaaatc
    84841 tttctacaac ttaaaaaaca aataattctt ttctaatgaa gatacatgat cgactattga
    84901 aagatagtcg agtatgatca cttagttaag taatcgaact ctctacttgc tgaaatatca
    84961 gcaggtagag agtttttttt gcaactagct tctgtatatg atgtttacaa agaatggtac
    85021 aagaaaagat catccttgaa aaaggaaagc taacaataac tagcatcata taagagcttt
    85081 taaggataga acagtagaat aggaatctat gagcaagtcc tctcgtataa ataaaatagg
    85141 agggaagtag cagcatattt ttcataaagt ggctctcttt cttcattttt actttggttg
    85201 ctcctatttg aaaattcgag cacactcaaa tacaataaaa agaaatggca agggagagga
    85261 ttagatgaaa aaagaagaaa aaatcgcgat tttacaagag atcatccgaa ttaaatcagt
    85321 gaatggaaac gaaggtgaag tagcagccta tttgaataag cttttggcaa agcatgacat
    85381 aacaggagag attgtttctt acagagatgg tagagataat ttgattgctc gttaccaaaa
    85441 gggccaatca ggaaaagtgt taggcctatc tgggcatatg gatgtcgtgg cggctggtga
    85501 tgaatcgtct tggacctatg caccctttgc agcagaaatc catgggaacc gtttgtacgg
    85561 acgtggtgct acagatatga agagtggctt ggcagcaatg gtgattgcca tgatcgaact
    85621 gaaagagtca ggaaaaccat ttaacggaac agtcaaatta ttagccactg tcggagaaga
    85681 agtgggtgaa ctaggtgggg aacagctcac gaaagcaggc tatgtagatg acttagatgc
    85741 cttgatcatc ggagagccaa ctaattacag tttgatgtat acgcacatgg gatcaatcaa
    85801 ctatacagtc acctcgcacg gaaaagaagc gcatagctct atgccagacc aaggttataa
    85861 tgcaatcaat cacctaaatg aattcattac gaaagcaaat gctgaaatga atcacctcgc
    85921 tgaaacaatc gagaatcctg tcctaggaaa aacaatccat aatgttacat tgatcagtgg
    85981 cgggaatcaa gtcaacagca tcccaagcca tgcgcagctc caagggaaca ttcgatcgat
    86041 tccagaatac ccaaatgata aaatcattgc attgctgcaa tcaatcgtca atgaattgaa
    86101 tcaagagaca gactatcatt tggaattgat gatcgattac aacaaaatac ctgtcaaagc
    86161 tgatccagat tctcctttga ttcattgcat tcagcaacaa ttcagccagc cattaccttt
    86221 agttggagcg gcagcaacaa cggatgcagc cgaatttacg aaagctaacc attccttcga
    86281 ctttgtcgtc tttgggcctg gtgtcgttac tttgcctcat caagtagatg agtatgttga
    86341 aatcgataat tatttggata tgattgaaaa atatcaagga attattttat cctacttagc
    86401 gtaagatttt tttaataaat gaaaaaacag agccagatta gcattatttt gctaatccag
    86461 ctctgtttat tcagcctatt tttctttttg aatatatcaa gcgttgtggc aacatagtga
    86521 tgactaatga aacaacgacg atagacaaca aacgatcaag gtagtctgta cctgcctgta
    86581 ccaagatcac acttgttgtc tgacttaaac ctaacccatg taatagctga acgatcatac
    86641 tggatcctga ggaggtgatc ccgccaaata actgaacggt aatgatcgaa gaaacgatcg
    86701 ttccaggtac cgacaaaatc aaagcgagta ataacagttt caagctgttt ttatctttgc
    86761 cgagatatct tggcaaaatc aatccagcaa gcaagcctgt gagaatttgg attggcgaat
    86821 aatacagaga aaaaatatct gtagtcactc cacttaaaat accgctggac aatcctgtca
    86881 gcattccagg tacaggccca agcaatgcac cagcaaaaat cgttccgata gaatcgagat
    86941 agattggcaa acgaagccat aaagcaatcg ttcctccaat ataattgatc ccaatacaca
    87001 aagccatcac tgtaagtacg cgagttgcca ttttgttttt attcattttg ttagcccatc
    87061 tttaaccttt ccaaatcttt cataatcgtt tcttcttgtg catttaaaag aacggttaaa
    87121 aactttcgga aaaaaagacg agtatctact tttgtcataa ttttactgtt agcaggtttc
    87181 tgccaaaatt cataacgatc aaccaatgtt tgtccacgac ttattccttg agtttccaca
    87241 gcagtgaagc ttttgaaacc ttcacagatg gccccctcga taaaataagc aacagctaac
    87301 ggatcattga tgacacatcc taaaattctt tcttgtttcc agtggaagtc aaaataaaaa
    87361 cgagtgatcg cttttagata ctctccctct tcaggattca tttgacagca gtattcgagg
    87421 atagtcggtg tcaagacgat ttctcttgtc acatctaacc caaccatttc tatcgtttgt
    87481 ttgagattct cgaatacata agaagctgct tcaggatcac accagtaatt gtattcagca
    87541 actggcgaac agttaccatg gcttttgtaa gttccaccca tcgagacaaa acgatccata
    87601 tgtttcccaa tatttgggtt gactttcaaa gcaaaagcga tattggtcaa aggacccaaa
    87661 gcaatgactg atgtatttgt cttttctttg aaataatcag ccaaaaaatc aacggcatgg
    87721 agaggttctg cctgttttct caagatcatc gggaaatttg tttcccctaa accatccata
    87781 ccatgagtgt cttgtgcact gatgaaaggt tgcttcaaag gtttttccat tccttgataa
    87841 acaggaatgt ctaaacgatc acagcgttcc aggcatttca gcacattctg cgtacctata
    87901 tctgcaggga cattcccaca aactacggta atagcaacta cttcgatatc aggggatttg
    87961 acagctaatg ataaagctaa agtgtcgtcg atccctggat cgcaatcaat aattactttt
    88021 cgcattattt ccaatctcct ttctttagag atacttagtt ttttattctg ggaagtttcc
    88081 gaaccagtcc aagacgagcg tcttgattat aatgcacgaa attagtatac tggcttttat
    88141 tgcgaatgta aatgctctct tacaagtaaa tctattgagg agaactttaa gtaaatgata
    88201 caatgaaaaa gaaatggaat ttacgtgtgc cgaaaataaa gaatcctatg gatcggtaca
    88261 ttggataata gttagattat agtttggcag aaagcggcgg gtgtacaatg gttatttttt
    88321 atttcattct tattctgata attataggga tcagttacca ttttctttct tatttttatg
    88381 caaagaaatt agtaagagcg ggagtacaga gaggatatga ctggtatgaa gacactggtc
    88441 atcgtttgat ggaccctgct gcagtcacgt cagtagaaaa aaaacgacaa aaattagaag
    88501 aagagtgtga aagcttttgg gagcagggaa cacccaaaac aatcaaaagt tatgacgggt
    88561 tgaaactagc tggtcagatg ttcatccatc caaataagca gaataaatgg gtcatttgcg
    88621 tacatgatta ccgaagcaca gggaaaagag acatgtctca tataggtaaa agatatgcag
    88681 agaaaggatt caacgttctt gtacctgatt tacgtgcgca cggagaaagt gaaggagaaa
    88741 tcatcggaat gggatggcta gatcggctag atttaattgc ctggatccaa ctcatcttag
    88801 atgaacagcc gaatgcgtcc attattttac acggcggatc aatgggagct tctacgatca
    88861 tgatggcaag tggtgaaaaa cttccatcag cggtcaaagg atttatttta gacagcggat
    88921 atgtttctgt ttatgcagaa ttccgatata tgttaagtaa aatcactgtc tttcctaaaa
    88981 aaatggtcat gagatatgcg aatcattatg cccagaaata cgccggttac tccttaaagc
    89041 aagcatcagc tacgagacag cttggcagca accatctgcc tttattggtc attcatggtg
    89101 aaagagatca ttttgtccct acagaagcag cgtacacgat tcaaaatgct acggcagggg
    89161 ataaagcact gctgcttgtt ccagaagcag aacacttgga agcatcgatg aaagatccga
    89221 aaacctattg gacagtgatt ttttcattta ttgaacaaag aataacgatg aattaatttt
    89281 ctcttctttt ttgtaagtaa ttcgaggata aataattgtt tatagatcag gagactgcta
    89341 aaataaggta ggaataaaaa aatatcagtt atttgaggag tgcggcatat ggaaatcaaa
    89401 gaagaaaaaa atcgtcttgc tttattcaat gatgaacaac aagagatcgg agaaatgaca
    89461 tggtcagatg cgggcccaga catcatgatc attgaccata catttgtaga tccagcatat
    89521 cgtggtcaaa agttagctga aaagctagtg tatactggag tagaattagc aagacgagac
    89581 gggaaaaaga tcattccttt atgcccttat gcaaaaaaag agtttgaaaa gaaaccagaa
    89641 tatcaagacg tcttacgtca gtcataagaa taacagctga aagttttgaa ctttcagctg
    89701 ttattccatt tatatagagt tgtcacggac ttttatctat gataaagtta aagagacgat
    89761 atcttttggg aaggaggctg cgatatgtat aatttaattt tgggaatcgt gatcgtttta
    89821 tcgattgtta tggtcattgc gattatgatg cagccaagca agcaaaatag tgcagcgagt
    89881 gcatttaccg gcggtgcgga tcaactattc ggcaaacaaa aagctcgcgg ctttgaagct
    89941 gtcatgcagc gatcaactgc agttatggga gctatctgga tgatcttatt atttgtccta
    90001 gcatatttat cttcgaagta gttgaataag cctctcgcaa aacggcgggc ggcttttttt
    90061 attgcaaaga gaacgcttaa gaagaagtta tgaaagaaac tatggtaaaa tgaggcgaaa
    90121 gaggtgagac gagtgagaca attaccaaaa cccctttatg caaaacatgg aaagcgagcg
    90181 gttttgctgc ttcacgctta ctcaggaagt ccaaacgatg ttcgaatgct cgcacgattt
    90241 ttggaaaaag cagattatac agtttatgcg ccgcttttta aaggacacgg aacaatggag
    90301 ccatatgata ttttgcaaga aaaagcagaa agctggtggg cagacactaa aaaagccatc
    90361 cattttttac agtctgagca gttttctgat attgccgtat taggattatc aatgggcggc
    90421 atctttgctg tacgagcact agaagaagaa tcagtgattg gcggcggttt tttctgttcg
    90481 cctttgtctc ctgtaaaaac aaacgtgcct gaaaactttg aaaaatacgt acgtcaagtg
    90541 ctgaagacag caggtaaatc agagaaagaa atcaatgaaa aagcagttgc ttaccgctcg
    90601 ttagctgaac aacaattaat ggatatacaa gatcaggcag caatcgtaga aagtaggttg
    90661 tcagatatcc aacagccgat atttttagct caagctggga aagatgaaat gatcgaccct
    90721 aatggcgtat ttgaaactgc acgcaagttg agccgtcagc gagtgacact tcgatggtat
    90781 cctgaaagtg gtcatgtgat tacagttggt acagcaagac gcgaattaga aaaagacgtc
    90841 ttggaatttt tagaaaaatt accttggaat gaggaataaa tatgacaaaa gaaacgataa
    90901 aagagaaaat tctctttttc atggaaagca gtcgcaaacg cagtttttct atggaagaaa
    90961 tcgctgaagg tttagggttc caaaaaagcg atgattttaa attactagta caaacgattg
    91021 ctcaaatgga aagagaacag agtatagttt tcaataaaaa aggaaaagtg aaacttcctt
    91081 taaaaccaat cttgatcgaa ggaacatttc gagcaaatga acgaggattt ggatttgtaa
    91141 cgattgatcc cgaagaagat gacatctata tcccaaaaga agcgactggt tttgcaatgg
    91201 atggagacac cgtagcaatc gatatcatga agcgagcaaa tacagatatg gatcgcggag
    91261 cagaggggaa agtagtcgag attcgagacc gggcgactac tcagttagca ggggaattcg
    91321 ttgcttattc agaagaagaa atacaagaaa cggatcttta cggcgttgtt atcccgaaag
    91381 atagaaagct caaccaattt aaagtatatg cagcagcaga gggagttcgt ccagttgacg
    91441 gaagtattgt gctgatcgaa ctgacccatt accctgaaaa agggtatgcg actagtttag
    91501 aagggataat caagcaagtg attggtcata agaatgaccc tggcatggat atcctctcaa
    91561 ttgtcgtggc taatggtatc cctacgaaat ttcctgatga tgtattgtct gaagcagatg
    91621 aagttccaga tcaaatcagc gaaaaggata taactggacg tagagattta agaaatcagc
    91681 tcattgttac gattgatgga gaagatgcaa aagatctaga tgatgcagtt acggttaaaa
    91741 aattagataa cggtaattat tttcttggag tacacattgc agatgtttct tactatgtga
    91801 cacaaggcag tcaattggac agagaagcat atgaaagagg gacaagtgta tatttgactg
    91861 atcgagtgat tccgatgatc ccgcaacgtt tatcgaatgg tatctgttca ctgaatcccc
    91921 atgttccaag gctgaccatg agttgcgaaa tggagattga tccaaatgga caagtagtct
    91981 ctcatgatat tttcccaagt gtgatccaaa caacagaacg gatgacctac acagctgtca
    92041 atcaaatctt agaagatcaa gacgaagaaa cgatgcaacg ctatgctcag ctcgtaccta
    92101 tgttccaaga aatgggtgaa ttgcatcgaa tccttgaaac gatgcgaatc aaacgtggcg
    92161 cgatttcttt tgaggatcgc gaagcaaaaa tccttgttga tgctgaagga cacccaaatg
    92221 atatccagct aagaagcaga ggaattggtg aacgcttgat cgaatctttc atgcttgcgg
    92281 ccaatgaaac ggtagctgaa cattataata aattaaatct gccgtttatt taccgaatcc
    92341 atgaacagcc aaaagaagaa aaaatgcagc gcttctttga ttttgcttca gctttgggca
    92401 ttctggtccg aggaacgaaa ggaacgatca caccaaaaga tttacagcat gtgatcgaag
    92461 gagtagaaga caaaccagaa gctgcagtta tcaatactat gttgctaaga agcatgcaac
    92521 aagcacgcta ttcagaagac aattttgggc attatggctt agcggcagat tactacacac
    92581 actttacatc tcctattcga cgctatcccg atctaatcgt ccatcgtctg atccgttctt
    92641 acagtgaaga taaaagtgaa agcaatcaaa gttcgtgggc tacggaactt cctgaaattg
    92701 cagatcatag ttctaagatg gaacgtcgtg cagtagaaac agaacgtgaa gtagattcca
    92761 tgaagaaagc agaattcatg atggacaaag tgggggaaga attcgatggg atcatcagct
    92821 ctgttgtgaa attcggcctg tttatcgaac tgccgaatac agtagaggga ttgatccata
    92881 tcaatgaatt gaaacaggac tacttccatt ttgttgaaaa tcatttagct cttgtaggtg
    92941 aaagaactgg tctgactttt aaaatcggac agaaagtgcg agtaaaagta gtcaaagctg
    93001 atccagatga acgagcagta gattttgaac ttgtttccgc agaagaggtt acagcgttgg
    93061 aacggccaaa aacacgaaat ggaaaatcag gaaaacgcga aggaaaacaa gcaggaaaac
    93121 gcccagaaag aaatggcaag aagaacaaac gatatagcga tagtaagcaa acgttcggta
    93181 agaaaaaagg gaagaagcca ttttacaaag gtgttaaaaa gaagaaaaag aaaaaataaa
    93241 atgagttgga ggaaacagct atgccaaaag gcgagggaaa attaattgca caaaacaaga
    93301 aagctcgcca tgattattcg atcatcgaca cgatggaagc agggatggtc ttgcaaggaa
    93361 ccgagatcaa gtcgatacga aacagccgga tcaatctaaa agatggattt attcgcgtcc
    93421 gcaacggaga agctttcttg cataatgttc atatcagtcc ttatgaacaa ggaaatattt
    93481 ttaatcatga tccgttgcgc acgagaaagt tattattaca caaaaaacaa atcatccggc
    93541 ttgaaaatga attgaaaaat actggaatca ctgttgttcc tttaaaagtc tatattcgta
    93601 acggctatgc caaggtattg attggtctgg cgaaagggaa aaaatcttat gataaacggg
    93661 aagatttgaa acgaaaagat atcgatcgac aaattgatcg aacattaaaa aatttctcta
    93721 gataatttca ttatcctttt tcgcatattc ggtgcttttg cttttactta atgataaaat
    93781 ttgttagcat ttaatctgat aaaacatgat tatcataggt tttttcttag atcaggagca
    93841 ccgtgtataa agaaaaattc gcttcgtttt tgactgaaat aaaaaagtgt ctaattttca
    93901 tgaaaatgtt ttgaattttc tgtttcatgg tggtatgata ccgtgtggtt agaaaccgat
    93961 tggaaaaaac cagatacgca catgaattga ttttatgtga atttcgggga aaagaggtga
    94021 aatggattgg atgaacgctc gctaacgttc cacattggac caatttggtt tgatggtaca
    94081 gtttgtataa tggtgttact gacctgtttg attgttttct tcttagctta cttttttaca
    94141 agaaatctta agttgaaacc aacaggcaaa caaaatgcga tagaatgggt cattgatttt
    94201 agtcgaggaa tcgtaacaga taatctgcct agaaaagaat tgagcaattt ccatttgttg
    94261 gcatttacat tgtttttatt tgtttttgtt gcaaacaaca tcggtttagt aacaaaaatc
    94321 gttttaccta acgaaacaac actttggaaa agcccgacag cagacccgtt tgttacttta
    94381 actttggcat ttataatgat cttacttact cacttatttg gggtgaaaaa gttaggcttc
    94441 aaaggatatt tcgtaaattc gttcttgaaa ccatatagtt ttatgtttcc gatgaaactg
    94501 attgaagaat ttacaaactt attaacactt gctttacgtc tttatgggaa tatttatgcg
    94561 ggggaagttt tgctaacctt gattgcaaaa atggtaataa gcttaggctg gttctcattg
    94621 ccgttagcaa ttccattaga gatggtctgg atcgcattct ccttgtttat cggaagcatc
    94681 caagcatttg tcttcgttac tttatctatg gtttatatga gccataagat tgaagtggaa
    94741 gaataatatt cgttttaaaa acaaactact atttatctaa tttaggagga aaacaattat
    94801 gaattatatc gcagcagcaa tcgcaatcat gggagcagca atcggagccg gttatggtaa
    94861 cggtcaagtt atctctaaaa caatcgaatc aatggctcgc caacctgaaa tgtctggtca
    94921 attccgtaca acaatgttta tcggggtagc tctagtcgaa gctgttccta tcttaggggt
    94981 agttgtcgcc ttgattctag tttttggtgt ttaattgaat cagtattaag gcagaaacaa
    95041 tttggtttgt tactgctttt ttacatgaaa actagaagta cttcaaagaa aggaaggctc
    95101 gcctatgctg aatcaattgg caattgcaga agttggcaat gcgatgttag gaaatgtcat
    95161 cgttgtcagc ggctcatttt tgatactact ggctctcttg aagcattttg cttggggacc
    95221 aatcagcgat attttgaaaa aacgtgaaga caagatcgcc aatgatttgg attctgcaga
    95281 acaatctcgc atcaactcag cgaaaatgga acaagaacgc gaacaacaat tgttagcctc
    95341 tcgttctgat gcagctgata tcatcaaaaa tgcgaaagaa agtggagaat taagccgcca
    95401 aaatattttg aaggatgctc aagaagaagc agctcgtcta aaaagtaaag cccaagctga
    95461 tatcactgta gaacgtgatt cagcgctgaa ctctgtaaaa gacgatgttg cagaactatc
    95521 tcttcaaatc gcggaaaaaa tcttgaacaa agaattatct ccagaaatgc acgaatcatt
    95581 aatcaatcaa tatatcgaag gtctaggttc ttcaaatgaa actagataaa tatacagtag
    95641 gaagacgtta cgggaaagct ttatttgaac tggcgatcga ttcaaataaa gcagaagaaa
    95701 tttatcaaga tctcgtaagc ttacgtcaaa tctatgaaca agtacctgga ttaggtaata
    95761 tgttaagtga tgttcgtcta gaacctcatg aaaaacgaga aatcatggac aagcttgtta
    95821 gtggctatga tggaatcgtt aaaaactttt tagaagttgt ttatgcgtat aaccgaatgg
    95881 atgatctttt gttcatgatc gaagaatatg aacgtcgata tgatgaacat caaggtttgc
    95941 tgttgggaag tgtcacaaca gcagttccac tttcagacga acaacgaata caattagaaa
    96001 agaatgttgc aaaaacaatg aactatcaat cagtcgaatt aaaacaaatc gttgattctt
    96061 ctattattgg tggcgcgatt gttgaagcta accatcgagt aatcgacgga agcattcgca
    96121 cgcaattaga aaatatgcgt aatcaattaa atagatagta caaagaggta ggtgaatcga
    96181 atggccatca aagcagaaga aattagtgcc ttgattaaag aacaaattga aaattatcaa
    96241 aacgtgcttt cagtcgaaga aatcggtacc gtaacttatg taggtgacgg gatcgctcgt
    96301 gcacatggtt tagaaaacgc gatgagtggt gagttgcttg aattttcaaa tggctcatac
    96361 ggaatggcgc aaaacttaga atcaaatgat gtaggtatca ttatccttgg cgatttcgag
    96421 tccattcgtg aaggagataa agtaaaacga acaggaaaga tcatggaagt tccagttgga
    96481 gaggccttga ttggacgggt agttaatccg ctaggtcagc caattgacgg actaggtgaa
    96541 atcgttactg acaaagctcg tccagtagaa gcaatggcac caggcgttat gcaacgtaaa
    96601 tctgttaacg aaccaatgca aacaggtcta aaagcaatcg acgctcttgt accaatcgga
    96661 cgcggccaac gtgaattagt tatcggtgac cgtaaaacag ggaaaacttc cattgcgatc
    96721 gatacgatca tcaaccaaaa aggtcaagat atgatctgta tctatgtagc aatcggacaa
    96781 aaagattcta cagttcgtac acaagttgaa acattgaaaa aatatggcgc aatggattac
    96841 acaatcgttg tgaatgccgg tgcgtctcaa ccagcgccgt tgctttatat cgcaccatat
    96901 gctggtactg caatgggtga agagttcatg tacaacggga aacatgtctt gatcattttt
    96961 gatgatcttt caaaacaagc tgttgcttat cgtgaacttt ccttgctgct tcgccgtcca
    97021 ccaggtcgtg aagcttatcc aggggatgtt ttctacttac actcacgctt attggaacgt
    97081 gcagcaaaat taagtgatga tttaggtggc ggttcaatga ctgccttacc atttgttgaa
    97141 acacaagctg gagatatctc tgcttatatc ccaacaaacg ttatttcgat cactgatgga
    97201 caaatcttct tagaaagcga cttgttctac gcaggtactc gtccagcggt tgatgccgga
    97261 ctatctgtat ctcgtgtagg gggttctgcg caaatcaaag caatgaaaaa agttgctggg
    97321 actttacgtc tagacttagc aagttaccgt gaattagaag catttacaca attcggttct
    97381 gacttggatg cagcaacaca agcaaaacta aatcgtggac gccgtacagt agaaatcttg
    97441 aaacaaaaat tgcatgcacc gctagctgtt gaaaaacaag tagtgatttt gtatgctttg
    97501 acacatggat tcttagatag tatccctgta gacagtattt tagacttcga acatgaatta
    97561 ttcgaatatc tggataccaa tcattcagat atctttgaaa caatccgtac aacaaaagat
    97621 cttccagaag aagaagcgtt gaacagtgcg attcaggaat ataaagacat gttcttagct
    97681 acaaaaggaa ataattcctc tacagaagat aaattgaaat ccattcaaaa tgcatagtga
    97741 ggtgagatga atgggtgctt cattaaatga aatcaagaca cgtatcgctt caacgaaaaa
    97801 aacgagccaa atcacgcggg caatgcagat ggtgtctgct tctaagttga caaaatctga
    97861 agcttcttca cagaagtttc aggtttatgc aacaaaagta agagaaatcg ttacgcattt
    97921 gactgctact cagttaaatg atattgcctc agaaaatcct agaggggata tcaattacaa
    97981 cagcatgttg ctcagccgtc ctgttaagaa aacgggttat atcgtcatca ctgcagatgg
    98041 cggtttagtc ggtggatata acagctcgat attgaaacaa acgatgtcta ttttagaaga
    98101 agaccaccaa tcacccgatg attacgtgat gatcgcaatt ggcggaactg gtgcagactt
    98161 ctttaaagca cgcggtatca atctagcata tgagctgcgc aatctatctg atcagccaag
    98221 ctttgatgaa gtccgcaaaa tcgtaaatat ggcaacaact atgtaccaaa acgaagtatt
    98281 cgatgaatta tacgtatgtt ataaccatca tatcaattcg ctaactagtc agtttcgtgt
    98341 agaaaaaatg ctgccaatct cagacttaga tccagaagaa gcaactactt tcgaacaaga
    98401 atatattttt gaaccttcaa aagaagaaat cttgtctcag ttattgcctc agtatgctga
    98461 aagtttgatc tacggtgcga tcgttgacgc caaaacagca gaacatgctg ctggtatgac
    98521 agcaatgaaa acagcaactg ataatgctgc taatatcatt gatgagctga cggtttctta
    98581 caaccgtgca agacaagggg ctattaccca agaaattacc gaaattgttg ccggagcttc
    98641 ggcactagaa tagtattgag attggaggaa aaaagatgag ttcaggcaag attgttcaag
    98701 taatcggtcc cgttgtcgac gtggaatttt ctttagacca atccttacca gatatcaaca
    98761 acgcattagt tgtttataaa aatgacgaaa ataaatcaaa agttgttctt gaagcagcgt
    98821 tagaattagg ggacggagtg atccggacca ttgcgatgga atcaacagat ggtttacaaa
    98881 gaggaatgga agtcattgat acaggcaaag caatctctgt tcctgtaggt aaagaaacat
    98941 taggtcgcgt attcaacgta ctaggagata cgatcgattt agaaacacct ttcccagaag
    99001 atgcggaaag aagcgaaatt cataaaaaag caccagcctt tgacgaatta agtacaagta
    99061 cagaaatttt ggaaacaggg atcaaagtta tcgatttgct tgccccatat ttaaaaggtg
    99121 ggaaagtcgg actattcggt ggtgccggtg ttggtaaaac cgtactgatc caagaactga
    99181 tccataatat cgcccaagaa catggtggta tttctgtatt taccggtgta ggtgaacgta
    99241 ctcgtgaagg taatgacttg tattatgaaa tgaaagattc aggagttatc gaaaaaacag
    99301 ccatggtgtt cggacaaatg aacgaaccac caggtgcacg tatgcgtgtt gctttgactg
    99361 gtttgacgat tgcggaatat ttccgtgatg tagaaggtca agatgtactg ttgtttatcg
    99421 acaacatttt ccgtttcact caagctggat ctgaagtatc agccttgtta ggacggatgc
    99481 cttctgcggt tggttatcaa ccaacattgg caacagaaat gggtcaattg caagaacgta
    99541 tcacatctac gaaaaaaggt tcgatcacat cgatccaagc aatctatgtg ccagccgatg
    99601 actacactga cccggcgccg gcaacagcat tcgcccattt ggatgcgaca accaacttgg
    99661 aacgtaaatt aacagaacaa gggatttatc cagcggtaga tccattggct tcttcttcaa
    99721 gtgctttagc acctgaaatc gtgggagaag aacattataa agtagcaaca gaagtccaac
    99781 acgtattgca acgatatcgt gaattacaag atatcattgc tatcttaggt atggatgaat
    99841 tatccgatca agaaaaagtg ttagtttctc gtgcccgcag agtccaattc ttcttgtcac
    99901 aaaactttaa cgtggctgaa caatttactg gtcttccagg ttcatatgtc cctgtagaag
    99961 aaacagtgaa aggattccgc gaaatcttgg aaggcaaata cgatgatctt cctgaagaag
   100021 ctttccgaag tgttggccga atcgaagacg tcgtagaaaa agcgaaaaca ctaggctact
   100081 agaaaggggt gccccatgga tcaatattta acagtaaatg tggtaactcc tgccggtttg
   100141 gtctatgatc accatgcagc tatcgtcgtt gcacgtacga cagctggtga gcttgggatt
   100201 ttgcctaaac atgcaccaat catcgttcca ttgacgattg atgaagttcg tgtaaaacga
   100261 acagattcag atacccacgt cgattggatc gctgttaatg gcggaatcat ggaagttcgt
   100321 gataatgttg tttcaatcgt tgctgatagt gcggaacgtg aacgtgatat cgatgtatct
   100381 cgtgcggaac gtgcaaaaca acgcgcagaa cgccaaatcg aagaagcaaa agcaaaacaa
   100441 gatacgaacg aattgaaacg ggcaactgtt gcgttgcacc gagcaatcaa tcgtatcaaa
   100501 gtatctaagc actcataaaa tgagatgcgg atgtttatcc gtgtttaaga acagtaaaaa
   100561 gaggttatga cagaagtggt cagcttcaag acataagaag gaatttacga aaattgcttc
   100621 tcaaattttt gtgaaatctc agcttatttt tcgaagaagc tacttctgga acaccgtata
   100681 tcggaggttg tgaaatcagc gttttgtcct gagtattaga gaaacaaatg gaaaatagtt
   100741 cctcatattt tgccgttttt tgagctaata ccgagggtca gatgattgaa caccgtttat
   100801 ttggaataag ggactgcgac aagac
//
LOCUS       DACEQH010000002        90802 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.002, whole genome shotgun sequence.
ACCESSION   DACEQH010000002 DACEQH010000000
VERSION     DACEQH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 90802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.002"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..590
                     /locus_tag="IW833_000097"
     CDS             <1..590
                     /locus_tag="IW833_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306248.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(830..913)
                     /locus_tag="IW833_000098"
     tRNA            complement(830..913)
                     /locus_tag="IW833_000098"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(877..879),aa:Leu,seq:gag)
     gene            1296..2513
                     /locus_tag="IW833_000099"
     CDS             1296..2513
                     /locus_tag="IW833_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1306249.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2697..3533
                     /locus_tag="IW833_000100"
     CDS             2697..3533
                     /locus_tag="IW833_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1306250.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3609..4757
                     /gene="nagA"
                     /locus_tag="IW833_000101"
     CDS             3609..4757
                     /gene="nagA"
                     /locus_tag="IW833_000101"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ1306251.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4978..6036
                     /locus_tag="IW833_000102"
     CDS             4978..6036
                     /locus_tag="IW833_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ1306252.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6055..7074
                     /locus_tag="IW833_000103"
     CDS             6055..7074
                     /locus_tag="IW833_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ1306253.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7176..7892)
                     /locus_tag="IW833_000104"
     CDS             complement(7176..7892)
                     /locus_tag="IW833_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1306254.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7861..8646)
                     /locus_tag="IW833_000105"
     CDS             complement(7861..8646)
                     /locus_tag="IW833_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1306255.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8637..9386)
                     /locus_tag="IW833_000106"
     CDS             complement(8637..9386)
                     /locus_tag="IW833_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ1306256.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9684..10208
                     /locus_tag="IW833_000107"
     CDS             9684..10208
                     /locus_tag="IW833_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306257.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10224..11723
                     /locus_tag="IW833_000108"
     CDS             10224..11723
                     /locus_tag="IW833_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ1306258.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11811..12413)
                     /locus_tag="IW833_000109"
     CDS             complement(11811..12413)
                     /locus_tag="IW833_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1306259.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12517..13473
                     /locus_tag="IW833_000110"
     CDS             12517..13473
                     /locus_tag="IW833_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ1306260.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13778..14734)
                     /locus_tag="IW833_000111"
     CDS             complement(13778..14734)
                     /locus_tag="IW833_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1306261.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14908..15468
                     /locus_tag="IW833_000112"
     CDS             14908..15468
                     /locus_tag="IW833_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306262.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15461..16285
                     /locus_tag="IW833_000113"
     CDS             15461..16285
                     /locus_tag="IW833_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ1306263.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16295..16912
                     /locus_tag="IW833_000114"
     CDS             16295..16912
                     /locus_tag="IW833_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ1306264.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16930..17613
                     /locus_tag="IW833_000115"
     CDS             16930..17613
                     /locus_tag="IW833_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1306265.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18296..19579
                     /locus_tag="IW833_000116"
     CDS             18296..19579
                     /locus_tag="IW833_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1306266.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19595..20482
                     /locus_tag="IW833_000117"
     CDS             19595..20482
                     /locus_tag="IW833_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1306267.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20499..21335
                     /locus_tag="IW833_000118"
     CDS             20499..21335
                     /locus_tag="IW833_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1306268.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21338..21961
                     /locus_tag="IW833_000119"
     CDS             21338..21961
                     /locus_tag="IW833_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306269.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21958..23250
                     /locus_tag="IW833_000120"
     CDS             21958..23250
                     /locus_tag="IW833_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1306270.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23609..23706
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23822..24403
                     /locus_tag="IW833_000121"
     CDS             23822..24403
                     /locus_tag="IW833_000121"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ1306271.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24406..25713
                     /locus_tag="IW833_000122"
     CDS             24406..25713
                     /locus_tag="IW833_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ1306272.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26129..26617
                     /gene="purE"
                     /locus_tag="IW833_000123"
     CDS             26129..26617
                     /gene="purE"
                     /locus_tag="IW833_000123"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ1306273.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26601..27734
                     /gene="purK"
                     /locus_tag="IW833_000124"
     CDS             26601..27734
                     /gene="purK"
                     /locus_tag="IW833_000124"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ1306274.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSIEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27747..29042
                     /gene="purB"
                     /locus_tag="IW833_000125"
     CDS             27747..29042
                     /gene="purB"
                     /locus_tag="IW833_000125"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ1306275.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29222..29599)
                     /locus_tag="IW833_000126"
     CDS             complement(29222..29599)
                     /locus_tag="IW833_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306276.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29855..30583
                     /locus_tag="IW833_000127"
     CDS             29855..30583
                     /locus_tag="IW833_000127"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ1306277.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30583..30837
                     /gene="purS"
                     /locus_tag="IW833_000128"
     CDS             30583..30837
                     /gene="purS"
                     /locus_tag="IW833_000128"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ1306278.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30839..31510
                     /gene="purQ"
                     /locus_tag="IW833_000129"
     CDS             30839..31510
                     /gene="purQ"
                     /locus_tag="IW833_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ1306279.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31511..33733
                     /gene="purL"
                     /locus_tag="IW833_000130"
     CDS             31511..33733
                     /gene="purL"
                     /locus_tag="IW833_000130"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ1306280.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33718..35157
                     /locus_tag="IW833_000131"
     CDS             33718..35157
                     /locus_tag="IW833_000131"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ1306281.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35189..36232
                     /locus_tag="IW833_000132"
     CDS             35189..36232
                     /locus_tag="IW833_000132"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ1306282.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            36229..36807
                     /gene="purN"
                     /locus_tag="IW833_000133"
     CDS             36229..36807
                     /gene="purN"
                     /locus_tag="IW833_000133"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ1306283.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36804..38339
                     /gene="purH"
                     /locus_tag="IW833_000134"
     CDS             36804..38339
                     /gene="purH"
                     /locus_tag="IW833_000134"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ1306284.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38355..39629
                     /gene="purD"
                     /locus_tag="IW833_000135"
     CDS             38355..39629
                     /gene="purD"
                     /locus_tag="IW833_000135"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ1306285.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39751..40665
                     /locus_tag="IW833_000136"
     CDS             39751..40665
                     /locus_tag="IW833_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ1306286.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40798..42363)
                     /locus_tag="IW833_000137"
     CDS             complement(40798..42363)
                     /locus_tag="IW833_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1306287.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42356..43807)
                     /locus_tag="IW833_000138"
     CDS             complement(42356..43807)
                     /locus_tag="IW833_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1306288.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTIEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43821..44837)
                     /locus_tag="IW833_000139"
     CDS             complement(43821..44837)
                     /locus_tag="IW833_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1306289.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45057..46145
                     /locus_tag="IW833_000140"
     CDS             45057..46145
                     /locus_tag="IW833_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1306290.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46199..47725
                     /locus_tag="IW833_000141"
     CDS             46199..47725
                     /locus_tag="IW833_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ1306291.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47727..48932
                     /locus_tag="IW833_000142"
     CDS             47727..48932
                     /locus_tag="IW833_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1306292.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49189..50166
                     /gene="guaC"
                     /locus_tag="IW833_000143"
     CDS             49189..50166
                     /gene="guaC"
                     /locus_tag="IW833_000143"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ1306293.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50430..51854
                     /locus_tag="IW833_000144"
     CDS             50430..51854
                     /locus_tag="IW833_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1306294.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52315..53424
                     /gene="pdhA"
                     /locus_tag="IW833_000145"
     CDS             52315..53424
                     /gene="pdhA"
                     /locus_tag="IW833_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ1306295.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53427..54404
                     /locus_tag="IW833_000146"
     CDS             53427..54404
                     /locus_tag="IW833_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ1306296.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            54429..56072
                     /locus_tag="IW833_000147"
     CDS             54429..56072
                     /locus_tag="IW833_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAQ1306297.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            56080..57486
                     /gene="lpdA"
                     /locus_tag="IW833_000148"
     CDS             56080..57486
                     /gene="lpdA"
                     /locus_tag="IW833_000148"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ1306298.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            57648..57923
                     /locus_tag="IW833_000149"
     CDS             57648..57923
                     /locus_tag="IW833_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ1306299.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            57929..58696
                     /locus_tag="IW833_000150"
     CDS             57929..58696
                     /locus_tag="IW833_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ1306300.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            58864..60693
                     /gene="typA"
                     /locus_tag="IW833_000151"
     CDS             58864..60693
                     /gene="typA"
                     /locus_tag="IW833_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ1306301.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            60954..62726
                     /gene="recQ"
                     /locus_tag="IW833_000152"
     CDS             60954..62726
                     /gene="recQ"
                     /locus_tag="IW833_000152"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ1306302.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            62840..63142
                     /locus_tag="IW833_000153"
     CDS             62840..63142
                     /locus_tag="IW833_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ1306303.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            63145..64308
                     /locus_tag="IW833_000154"
     CDS             63145..64308
                     /locus_tag="IW833_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1306304.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            64334..67762
                     /locus_tag="IW833_000155"
     CDS             64334..67762
                     /locus_tag="IW833_000155"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ1306305.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            67783..68907
                     /locus_tag="IW833_000156"
     CDS             67783..68907
                     /locus_tag="IW833_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306306.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            68897..69334
                     /locus_tag="IW833_000157"
     CDS             68897..69334
                     /locus_tag="IW833_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1306307.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            69318..69656
                     /locus_tag="IW833_000158"
     CDS             69318..69656
                     /locus_tag="IW833_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ1306308.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            69775..70335
                     /gene="rsmD"
                     /locus_tag="IW833_000159"
     CDS             69775..70335
                     /gene="rsmD"
                     /locus_tag="IW833_000159"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ1306309.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            70332..70823
                     /gene="coaD"
                     /locus_tag="IW833_000160"
     CDS             70332..70823
                     /gene="coaD"
                     /locus_tag="IW833_000160"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ1306310.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            70813..71856
                     /locus_tag="IW833_000161"
     CDS             70813..71856
                     /locus_tag="IW833_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1306311.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            72015..72743
                     /locus_tag="IW833_000162"
     CDS             72015..72743
                     /locus_tag="IW833_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ1306312.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            72859..73104
                     /locus_tag="IW833_000163"
     CDS             72859..73104
                     /locus_tag="IW833_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1306313.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            73101..73319
                     /locus_tag="IW833_000164"
     CDS             73101..73319
                     /locus_tag="IW833_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ1306314.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            73455..73994
                     /locus_tag="IW833_000165"
     CDS             73455..73994
                     /locus_tag="IW833_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ1306315.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            73987..76260
                     /locus_tag="IW833_000166"
     CDS             73987..76260
                     /locus_tag="IW833_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ1306316.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            76298..77344
                     /gene="holA"
                     /locus_tag="IW833_000167"
     CDS             76298..77344
                     /gene="holA"
                     /locus_tag="IW833_000167"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ1306317.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(77433..77687)
                     /locus_tag="IW833_000168"
     CDS             complement(77433..77687)
                     /locus_tag="IW833_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ1306318.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(78087..78356)
                     /locus_tag="IW833_000169"
     CDS             complement(78087..78356)
                     /locus_tag="IW833_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306319.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(78826..79281)
                     /locus_tag="IW833_000170"
     CDS             complement(78826..79281)
                     /locus_tag="IW833_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ1306320.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(79419..81347)
                     /locus_tag="IW833_000171"
     CDS             complement(79419..81347)
                     /locus_tag="IW833_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1306321.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(81544..82377)
                     /locus_tag="IW833_000172"
     CDS             complement(81544..82377)
                     /locus_tag="IW833_000172"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ1306322.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(82569..83273)
                     /locus_tag="IW833_000173"
     CDS             complement(82569..83273)
                     /locus_tag="IW833_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1306323.1"
                     /translation="MTETTLYIVRHGKPMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(83296..83922)
                     /locus_tag="IW833_000174"
     CDS             complement(83296..83922)
                     /locus_tag="IW833_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1306324.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            84248..86623
                     /locus_tag="IW833_000175"
     CDS             84248..86623
                     /locus_tag="IW833_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ1306325.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(86647..87138)
                     /locus_tag="IW833_000176"
     CDS             complement(86647..87138)
                     /locus_tag="IW833_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ1306326.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            87325..88245
                     /locus_tag="IW833_000177"
     CDS             87325..88245
                     /locus_tag="IW833_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1306327.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            88399..88743
                     /locus_tag="IW833_000178"
     CDS             88399..88743
                     /locus_tag="IW833_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ1306328.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(88933..89433)
                     /locus_tag="IW833_000179"
     CDS             complement(88933..89433)
                     /locus_tag="IW833_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1306329.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            89961..90044
                     /locus_tag="IW833_000180"
     tRNA            89961..90044
                     /locus_tag="IW833_000180"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:89995..89997,aa:Leu,seq:cag)
     gene            complement(90368..>90802)
                     /locus_tag="IW833_000181"
     CDS             complement(90368..>90802)
                     /locus_tag="IW833_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ1306330.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        29441 a        15662 c        19444 g        26255 t
ORIGIN      
        1 tttttttaga catgaatatc ttatttttat tgctattttt cttaattttc cttctaacat
       61 ataggaaatg tcaaaactat tatgtgcgta tattgactgc attaccgttt ttcttaaacc
      121 tgattattta ttttggtaat acaatgggtc aaagctttac ttacgtcaat ggaaataaaa
      181 gggcaatgat ttggagtagt agtaacctga ataatctctt tacagagtta ggaacgaaac
      241 tgtcactttt ttatccaggt acttggatag ctactttagt agtcttggca ttattactat
      301 gtttaattgt tggaatttat ttaagttttg acaataagaa aacttctatt tttttagtta
      361 ttttgatgat aatgggattt tgttcaaggt taattatggg attttctccc acagtatggg
      421 cttccggaat gcggacgtac tatatacttt atgttgttat cgccatctta gtactaatgg
      481 cagttaaaga gctaatgaaa agcatgagtg tacaaaaaaa tgaatttatg caatttggat
      541 taaccgtgct tggtatttgt acctttataa taacggtgat aaacagataa tatacaaaaa
      601 atccctcgct ttagtattag gtgagggatt ttactcgttt ttcgattaat tgttttaaca
      661 cttgcaagtt tctacgagta gcttgtttcc gaccttcata tactcacctc aggctgttta
      721 catgattgta cgttcttttt gtaaaggata cagtgatatg tatataatgg cataaagaaa
      781 cgttcaaaca aaaaaactcg tgaagcaaaa ttaacttcac gagttgtagt gcagatgatg
      841 ggactcgaac ccacacgagc taacgctcac atgaccctca atcatgcttg tctgccaatt
      901 ccaacacatc tgcgtaataa taacacacta atgatagcga ggttttacat agttgtcaag
      961 ctcaaaaatg ggaggactta ttaagatgga gaaatatgac ggagaatttt caggactggg
     1021 aatgatctta ggtgtattga ttggacttgc attcggacgt tttttgcttg gttttatgct
     1081 aggaatcatt tgtggaattg caatggattg ggcagcgaat ctatggaatg actatcatga
     1141 caattaagtt ctcaacgctt gtttgtgttt ttttaatact tacgtttttc ctaaataagt
     1201 ggcaaaattt tctaggatgg agtatgatat tgaaatagta aataacaaaa aatttggaaa
     1261 gttctaactt tttgaaatga gggattggct aagtgatgag agaatttgaa aaatccaaca
     1321 aattagaagg tgtcagttac gatgttcgcg gtcccgttct agaagaagcg gagcggatgc
     1381 aagaagaggg cattagtatt ttaaaattga acacgggaaa tcccgcacct tttggatttg
     1441 aagcaccaaa cgaagtcatt cgtgatttga tcatgaatgc ccgtgactca gaaggctact
     1501 cagattccaa ggggatcttt tctgctagaa aagcaattga gcaatattgc caactgaaac
     1561 attttccgaa tgtgacgatc aatgatatct acaccggcaa cggagtgagc gaattgatca
     1621 cgatgtgtat gcaggggttg ctggacaatg gagatgaagt actggttcca atgccggact
     1681 atccgttgtg gacggcttct attgcattag ctggaggcac acctgttcat tatatttgcg
     1741 atgaagaggc agaatggtat cctgatattg atgatatcaa atcaaaaata acgagtcgga
     1801 caaaagctat tgttatcatt aatcctaata atcctactgg ggcactttat ccaaaagaat
     1861 tattagaaga aatcgttgaa gtagctcgac aaaataacct gattatttat tccgacgaaa
     1921 tctatgatcg cttagtgatg gatggtctag aacatattcc gattgctaca ttagcgcctg
     1981 atttgttcgt tgttacgctg aacggtctat ccaaatctca tcgtgtagca ggatttcgag
     2041 taggctggat ggtcctaagt ggagataagt cacacgtcaa aggctatata gaagggctga
     2101 atatgttgtc ctccatgcgt ttgtgttcca atgtattgtc tcagcaaatt atccaaaccg
     2161 cgctaggcgg ttatcaaagt gtcgatgagc tgctcctgcc aggcggaagg atctatgaac
     2221 aaagagaata tatctataat gcaatcaatg atattcctgg tctttcagcc gtaaaaccaa
     2281 aagctgcttt ttatatattc ccaaaaattg atacaaagcg attcaatatt ttggatgatg
     2341 aaaaattcgt tttggacttt ttacatgaac atcatattct acttgtacat ggtggtggat
     2401 ttaattggaa ccagccagat cattttcgaa tcgtttatct tcctaaaatg gatgatttga
     2461 aacaaacagc taagaaaatg agagaattcc tagcaaccta tcgccaaaaa tgattttaaa
     2521 aaccaaattt ttcagtagga gggccacaga caaacacact gaggctctct ttctagtgga
     2581 gttttcttaa ataactaaag tatattattt agttgcaagt cttttcttga agtttgcgac
     2641 ttcctaaagt aaaatacgaa tagaatgcga atacgcatat tttggggagg atacttatgg
     2701 aaacgttaat cgagtccttt ggaacgatca ttaaagagat tcgaaaagaa caaaaaatga
     2761 cgcaaaaaat gctttcgcat gatatttgtt cacaaagtgt attaagtcgg atcgaaaata
     2821 atgaggaact gccgaatgta ttggtgatga tgcagatttg tcaacggcta ggggtaacga
     2881 ttgatcatgt gatgaatctc tctcgaaaaa ctactcggac aaacgataaa ttatttgaac
     2941 tgttggatag ttgtttccaa aaacgtgatt atcggaagtt agaacaagta ctaaaatctt
     3001 ctgatatttc tgaaaatctg tatcaagcaa ctgactttca acgctattat tattatctcg
     3061 gtgtttgcga attcacgttg cgtcaaaata tctctcagtc cctttattat ttgaaagaag
     3121 cattaagcta ctctagccaa aaagaaagaa tccatgtttc tgacacagaa atacaactga
     3181 ttagttgtat cggaaaaata tatggtgtgg caggaaaaac ggctgaagca cgctactatt
     3241 tgagtcgaag catccaactg ctgcatcaat cagctgatga acgatcaaaa agtgaatttt
     3301 cgcaaatttt ttataattat ggaaatttct tattccatca aaatgaaatc gacgaagcgt
     3361 tagaacaggt gaatcaagga atctattggg ctcaagagaa gaatagctac tattacctca
     3421 atgatttatt tgtgcttaaa agcttaatat acaagcgtaa agaagaacca gaaagagcag
     3481 cattctatga aggcttagca caagctgtga aacaaatttc aaaaaatttg taaatggtgt
     3541 agaccattct tgacttcttc tctaaataag agtactttag aggtagaaaa acaaagggag
     3601 agaatagaat gagaactttt atctatgctg acaaattctt tttagcgtca ggtgtcaaac
     3661 atgcaggcta tcttgaaatt accgatggac tttttggtga atacaccaaa gaactacctg
     3721 agggagaagt caacatcatt gaccaaagcg gcaaatggat tgctcctggt ttggtagata
     3781 cacatattca cggctatatg aatcatgatg taatggataa tgatgcagaa ggcatcaaag
     3841 tgatgtctga aggtctgtta tcttgtggag tgacttcatt tttaccaaca acattgacgt
     3901 caagcaaaga acgtttaaaa gatgtagctg agacaatcgg caaaatgtat caggaagcac
     3961 caggagctaa aatcaaagga atctattttg aaggtccttt cttcacagaa gaacacaaag
     4021 gggcacaaaa cccaagttat ttcggggatc ctgatttgga tactttccat gaatggcaag
     4081 aagcttcagg tggtttaatc aaaaaaattg ctttagcacc ggaacgtcaa ggtgtagaag
     4141 agtttgtcaa acaagtgact gaagaaggag tagttgtttc tctaggacac agtaatgcta
     4201 cactagaaga agcgcaaaaa gctgttgaag caggcgcaag tgtatttgtc catgctttca
     4261 atggtatgcg tggattgaat catcgcgaac caggtatggt gggtgcgtta ttatctttag
     4321 aacatgtctt ttctgaattg atttgtgatg gccatcatgt acatccaaat gctgcgggaa
     4381 tcctaatgga aaaagcagga catgatcatg tcgcattaat tacagactgt atgatggccg
     4441 gtgggatgcc tgatggaaat tacaatttag gcgaatttcc agtagtagta aaagaaggaa
     4501 cagcacgtct agaatctggc aacttagctg gaagtatatt gaaattgaaa gaagcaatta
     4561 aaaatgttgt agactgggga ttagcaacac cagaacaagc agtcatgatg gctagtctga
     4621 ttccagcaat cagctgtaaa atcgatgata catgtggaaa aatcgctcaa ggacgcgatg
     4681 cagactttat cgtattgaac cctgatatga cattggaagc aacttatcta gatggcgtgg
     4741 aacgtttcca cgcataatat aatttaagtt aaccttaaaa ctaaacttct taagtttggt
     4801 tttaaggttt tttttgtgtt ctagtaaaaa tcactttaca taagtttcaa aagtttcttt
     4861 aatttcctat tgttcaataa gtaacaatgt aaagataaaa ttaaaaatgt taatacgtat
     4921 tagcaaaata aaagcttttt ttattgtgaa taaatgaaca taattaggag gagaaagatg
     4981 aacttatttg acatttttcg atcgactttg acagatatgt cgattgtcag tgcaatcact
     5041 tcaacagttt ttattatatt attagggttt gtttgtagaa aaaaggggat tttttctgaa
     5101 tctgttggaa aaattctatc gaaagtcgtc ttaacagtgg ctttgccagc actggcattt
     5161 cgagctttca tgcaagatat caatacagaa ttactgatgc aaggaatcaa tgtcttgatt
     5221 tggggattag ttatttatat tattcttatt ctagtttcaa aacctttttt cttaagctat
     5281 aaaggggata agcaaacaac gttgcgtgtc ctgacgatct ttggttctac gacctttttc
     5341 ggaacgccta tcgtcagtgc gatttatggt cctgtcggcg tgatgtattc ctcaattttc
     5401 aatattggct accgaatctt tttgtattct tatggataca taaaaatgag tggcttgaag
     5461 atggaagcta aaaatatcaa agcgatgttc gtcaatccaa tcattatcgc tacttttgcc
     5521 ggccttttca tttggctgtt ccaagggtat ctgccacaag tgtcagttac aggtgcagaa
     5581 ggagagataa gccaagtagc gtttttgcga attgaccaga ctgcaccgtg gctatttcag
     5641 ccgatggatt atttagctgg tttaagttca ccattggcgt ggctagcaat cggcgcaaca
     5701 ttaggtgaag taagcttcaa aaaagctgca tctgaaaaaa cttcatggta ttacagtcta
     5761 gtcaaagtgg tatttgtacc tgcattgaat atcgtgattc tagcagtttt gacggcaacg
     5821 aatattctac cagtcaattt tgaagcactg gcaacaatcg ttatcatgat ggctacacca
     5881 gctgcaacag tggcagcagc ctatgctatc agttttgata gggaggcact tttagcctcc
     5941 aatgcatcct tactgtcaac tatttttgct gtggtactga cacctgtatg gatcgttgta
     6001 ttacaaatga ttcatggcat aggtttattt tcataagaaa tggaggaaga aagtatgaga
     6061 atcgcatgtt acggtgttcg tccgaacgag atagattttt ttgaaaaatt gaatatttac
     6121 cattatgatc tcagtcttta tgaagaactg ttgacacatg acaatatcgt gtcagcaaag
     6181 gaccatgatg ctgtattgtt gaggggaaat tgtatagcag atgagaccaa tcttgcaaaa
     6241 atgcaggaat atgggattcg ttatgttttt acacggactg tgggagtcaa tcatatcgat
     6301 ctacaagcgg cagctgattt tggtatgaca gtagcccgtg taccatcgta ttcgccaaat
     6361 gcgatagcag agttatcttt gacttttgcc atgatgcttt tgagaaatac tgcgtatacg
     6421 acgatccgaa catctttcaa agattttcga gtggatgagc aaatgtttag tcgtgaaatc
     6481 agaaattgca cagtagggat cataggaaca ggcaggatag ggttgacgga agctgttttg
     6541 tttaagggac taggagccaa agtgatcggc tacgatattc atcagacaga agctgctaaa
     6601 aaagtattga cgttccactc gcttgaagag ttattagcca aaagcgatat tgtcagtatt
     6661 catgtacctc atatcccagg tgaaaacgat caaatgatca atgaaggatt tttagctcaa
     6721 atgaaaaaag gtagcatctt gattaataca gcccgtggag agcttcaaga taatgaagct
     6781 attttaagag ctttgaaaag caatcattta gctggttttg caaccgatgt ttttgcaaat
     6841 gaaacagagg tttttttccg atccttcaag ccatgggaga cgattccaga ccctgctatc
     6901 cagcaattag tagaaatgta ccctagagtt cttgtaaccc cgcatgtagg ttcaaataca
     6961 gatgaggcat tatcaaatat gatttcgaca agtttcgaga actttcgtga aataatagaa
     7021 actggaaaaa cgaaaaatga ggtatctctt cctaaagccc gtcaattaag ataagaaaac
     7081 atggatattc gtcaataaaa tgtttaaaaa tagtccaagt aaacagttgt tacttctgta
     7141 tacttggact attttgtatg ggataattcg atgactcaac agtaatctgt gagctttttc
     7201 cgcccattct ttgtcaattc ataacaggtt agcggccggc cgatttccaa atatttgatt
     7261 ctttcgttca atatatcttg ttctactaaa aaattcaaat atttcttgac ggatatacgt
     7321 gacagtgagc ttttttgagc cagttctttt gttgaaaaaa tagtatcttc ttttagaata
     7381 aatgcactta ttttttttaa agtcagtttt gacagacctt taggcaaatc gatgaatgat
     7441 ggtatttcgt gaatgggagt ggtgtggttg aatacttgat cgatcaattt ctgatcggct
     7501 ttttcagtcg tttggattgt ctgagagaaa gcaagatact tttcgatcgc cagctgaaaa
     7561 cgttgcaaag tgaacggttt gatcaaataa tctaaggcac cgtagcggat cacattcttt
     7621 atggtttcga tatcatctgc agcagtgatc aaaatcactg ggatatcatt tccttgctgt
     7681 ctaagccaag ataaaaaatg gataccactt tcgccaggca tgtatacatc aagcagcagc
     7741 agatctgcgt ttttttgtct gaaatatttt tttccttctt ctacatttct gaaactttcg
     7801 attacgttca tattagggat gctttccaca aaagtacgat tcaattctgc aaccattgga
     7861 tcatcttcga ttatcaggac atttgtcatc attttgatct tccttttttg gtaattctag
     7921 atagacgatt gtaccgccat ttgtttgttt ggaaaattct aaaaatccac cgtggcgttt
     7981 gacgattgaa tggatgagat tcaatccaaa cccacgattg attcccttcg tcgaaaaacc
     8041 tagcgtaaat attttttctg agatttcttc tgatatcccg acaccgtcat cgcagacggt
     8101 cacgatgaat acttcagctt ctgtgtcata gtttaaatga atccggacct gtccgtttct
     8161 ttcatgtttt tgtagcacgg cttcttttgc attatcaagt aagttcccta acacttgcaa
     8221 aagtgaatgg atggagtcat ccatctccat tttagggata aaagaatcgc tttctaacag
     8281 catttcaaca gattgttctt tggcttcgtt tattttgcct aataaaaaac cggcaattgc
     8341 aggtactttg atcagacttg agacttggcc aatctctttt ttataatttt gatttagctg
     8401 ctggataaaa tgggtgactt cttcatactt cttcaaatca atcaaaccag aaatgacatg
     8461 aagtttattc atgaattcat gggtttgagc tctcatggag tttatatatt gtttcgtccc
     8521 cccaagctcc tccattaatt tcattacttc tgatcgattt cgtaaagttg cgactgctcc
     8581 taaaaaacgg tcattgctaa aaagaggtgt aacgctagaa atagtttcta tcccatttaa
     8641 atacatggat tgagtccgaa cggtcgtttg atgctcaatc gtctctttaa acaaaacgtc
     8701 atatagatta tcactgattt ttttcctact tcgaaatcca atagctgctc tgtttggtag
     8761 aaaatgtttt ttgcaggtgt attgatcaag aggatagttt tatcagaaga gatggctaag
     8821 attccttcat ctacttcatt ttcgataatc gttttttcac ttaactgttc tgctatttct
     8881 tgtggttcaa gtccaagcag tacttttttc attttttggg caagaaaaac ggcgcctatg
     8941 atgcctacgc taagtccaat aatcaatgaa aaaacaatca ttttttgtcc atctttgact
     9001 tgattgtcaa tcgtgttgtt tgttaagcca acacaaactg caccaataat ctcttgatca
     9061 taataaatag ggacgaaata gcggaagccg aaccctaaca ttccttcttt ttcagagaaa
     9121 aattcttgtc ctccaatcac ttttttcaca tcttcatgac ttgagaaagg aagaccgatg
     9181 taacttgggt ctgcgtgaga atatcgaatg ccttcagggt ccatgacaac gataaaatct
     9241 aaattcgatg caatcatgat ttcatttgtt attccttgaa tcgcatgtgt cgggtcacca
     9301 gtctgcagct ttttttggat gagtttatca ttggcaacga ttttagcaat gctagagatt
     9361 ttatctgaca cactagatcg gttcatcgaa agaataaaat tcctgatcaa tatggcagaa
     9421 acaatcaaag aaacgaatgt ggagaaacaa acgacaagga tgatcgtgtt ctggagcttt
     9481 aatggttttt tcttaaaaaa aggaaacata ggctacctcg aaagaattta ttgtgaaaat
     9541 tttaacatga aaacaaaaat ttaacaaatt gtcaacgata aacacaacct tcctataaaa
     9601 aacaccactg agtcggtaaa agaagctata cagaattttc aaacattggt atgatagacg
     9661 tagaaatgga aggaggcgtg tatatgatag ggggaatcgc acttgagacg atttattggt
     9721 atacattgct gatttgtgca ggattggcag tcctattgat tatttttgga gatgtattcg
     9781 attttgatgg cccgcttgat ccgatgctta ttatcccttg gttaactttc acaagcttat
     9841 ttggttttct aggtgagcgt ttaacagatc aatcatcttt atttttattt attgctagtg
     9901 gaggcatatc gatagtgttg gtatttttgc ttaatttcta tatattgatg ccgatgaaac
     9961 atgccgaatc aacgatgtcg tcttctgaaa aaactctgga aggacaagta gctgttgtga
    10021 tcacaccgat ccctgtacga ggtatgggag aaattcagtt gaaaagtgtt accggttcca
    10081 ttagtcgtcc agcttgtttt tacagccccc aagaacagac agctgagcgg ggaacgaaag
    10141 tattgatcat tgagatcaag gaaagaatct gttatgtcat gccttatgaa agcatgataa
    10201 aagcttaaag gagcgtgtgt taaatggaaa taccagttgt ttttatcgcg atcgcaatcg
    10261 cagctttgat tttactgatg ttgctgatcg tctttgtagc gaagtaccag acagcaaaac
    10321 cggatgaagc gttgatcatc agtggaagtt atttaggaag caaaaatgtg cacattgatg
    10381 aaagcaataa caaaatcaaa attgtgcgtg gaggtggcgc attcgtcttg ccagtttttc
    10441 aacgttcgaa tcggatcagt ctattatcga gtaaattgga cgtctccacc cctgaagtgt
    10501 atacggaaca aggcgtaccg gtgatgtgtg atggtacttc gatcatcaaa atcggttcat
    10561 ctgtagaaga gattgctaca gcagcagaac agtttttagg caagacaaga gaagaactgg
    10621 aaaatgaagc aagagaagta ttagaaggac acttgcgctc gattcttggt tctatgactg
    10681 tagaagaaat ctatcaaaat cgcgataagt tcagtcaaag cgtacaagaa gtcgcttctg
    10741 tagaccttgc aaaaatgggg ttgatcatcg tttctttcac tatcaaagag gtcagagaca
    10801 aaaacggtta tcttgactct ttagggaaac caagaatcgc ccaagtcaaa agagatgcag
    10861 atatagcaga agcggaagct ctaaaagaaa cgcgtatcaa aaaagctcaa gcagaaaaag
    10921 aatcacaagc agctgaatta cagcgacaaa cagagatcgc agaatcatta aaagaaaaag
    10981 aactgaaatt agcaacctac aaacaagaac aagatgttgc taaagcaaaa gcagaccaag
    11041 cctacaattt agaaagtgcc cgagcacaac aacaagtaat cgaacaggaa atgcagatta
    11101 aaatcgtaga acgtcaaaaa caaatcgaac tggaagaaaa agaaatcaca cgtcgtgaaa
    11161 agcaatatga ttcggaagtg aagaaaaaag cagatgccga tcggtacgct aaagaacaag
    11221 aagcacaagc tcaaaaagtc aaagaagtga ctgaagccga agcagaacga ttccgtgtgg
    11281 aagcactggc agaagcggaa gccaataaga cacgtttagc tggacaagca gaagccgaag
    11341 cagcactagc aaaaggaaaa gcagaagccg aagcaaaaca aaagatcgcc aatgccttca
    11401 aggaatatgg cgaagcagct gtcttgagta tggttatcga tatgctaccg caattgatgc
    11461 gcgaagcagc acagccgcta ggaaatatcg aaaaaatctc tgttgtcgat acaggaagca
    11521 gttcaggaga aaccagtgga gcgaaccgag tgacaaatta tgccacaaat ttgctttctt
    11581 ctactcaaga aacactaaaa gcgacaacag gacttgactt gaaggaaatc gtagagaatt
    11641 tgtcgacacg tgggactact agccaagtaa atatgtatga agcgccaaaa gaggcagaac
    11701 aaaactcgat gaaggaagaa taaagtagta cacttcaccg ttcaatcaaa aatggaggaa
    11761 gactttttaa agttcttctc tccatttttt tattttattg caaaagaaaa ttagtcctcg
    11821 ctatttttct tttgtttttg gatcttagct tctatctctt gtaaatttgt tcttagcaat
    11881 cgttctttct tttggagttc gaccaaatct tctgatagtt cttccttctt ttctgtttca
    11941 ggaggacgaa gaaagaaatt gataccgatt gcaatgaatg tccctataaa agtatctagt
    12001 acacgttgga tagcatacaa agcggattcg ccttgaggga tgctcaacgc aatcagcaac
    12061 aacgtagcta ttgcggaaat gataccggaa ttattgttaa taccatctga cacgacgatc
    12121 acaataataa ctagaatagg aagcaagatc aattctacca aaaaatcatt gtgaaaatag
    12181 cttttgacaa aaaaataaat gatagctaat gtaccaccta atgtattgcc gatgatccta
    12241 gactttccaa aagaaacggt tgtagtcaag tcctgacgca aagaaaaaac agcagataat
    12301 gcagcaatca tcgggattcc tcgttgagtc aaatgaaaaa acaagataca taaaaaaaca
    12361 gctaaggcac ttttgatcgt acgcatacct agtcgaaaac ggccaaattg catagagatt
    12421 ctcctttttg gtctttttac tatcatacct aaacttttgt ctgatagcta gtttttcatt
    12481 attcaagtat aattaaaata aaaaaggggg aagtaaatgc gtataggaat gcgaacagtc
    12541 aaaacagtga tcagtgtctt cttttcgatg gtcacagcgg gtagcttatc gctgttgtat
    12601 tggccagctg cagggatcat tgcattatta agtgtaggaa ataccaagcg ctctacattg
    12661 cgtacgggca tgaatcgaat cgcagccttt atattagcta cggcaacagc actgttcagt
    12721 ttttttgctg ttgggtatac tatttttggc ttcagtcttt ttttattcct gttcatcccg
    12781 cttgcctctc gcttcaaatt aaccgaaggg atagtagtaa attcggtttt ggtcactcat
    12841 tatttagtgg aggaaaacat gtcttggcag ctgatcggca atgaagcggg attaatggcg
    12901 attggattag tatttgcttt gcttgcaaat gtttacatgc cagatcgtac gaagcagctg
    12961 aaggaaaacc aaatacagat cgaaaaagaa tttcgaaata ttttgagaca aatggcagaa
    13021 tttcttttat cagaaaataa agaagatgtg cagatcaaat gcgaacattt actgacattc
    13081 attcgcgaaa gccaagaaga tgccagagaa catcaagaaa actactggct cagacagccg
    13141 ctatactacg aaacttactt ctctatgaga agagcacagg ccaatgtgat taaagacatg
    13201 ctggaaaatc tagagagaat ccagcaacca gcttattatg gaaaacatat ctatggttta
    13261 ttgatttata ctgcagaaac attttcagag tcaaatgatg gcagacaaat tttaacacgt
    13321 attgaagaag tttatgtatt gtatcggcaa atgcctctgc cgactagtcg ccctgaattc
    13381 gaggatcgag cagaattgtt ccagtttttg cagtctttta aaagttttat tgaaatcaag
    13441 gcagaattct cccaacaaaa aatccaaaaa tagaggttgt gaattcagct aatattcagg
    13501 tcaaaaagct gatttcacaa cctcttcttt aaacggtgtt ctcctgtcaa ctcctcggtc
    13561 acaagtcaca atattcaaca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
    13621 ggagctgaac ggtaggctca caacctcttt cttaaacggt gttcaaaagc tttcctctgc
    13681 ggtaataagt ccagccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatt
    13741 acttgatgca gaacagcttt ttcacaacct tttaaaatta catatttttc catagaactt
    13801 gcatttgatc caagatttcg atgacatctt ttgtcaagaa gagagagcga ttgggcaagt
    13861 caccttcaat catgtgtacc atattcttta tttcgtaatt cattgcttca gatgtattcc
    13921 cgctttcgat gaattccttt gtaccatctg taaataagat ctccgcacga tcagcacgcg
    13981 ggtaatcagc aatcgtcaca taaccgttct caaaggcaac gataccgact tttggcattt
    14041 ttgcctgaaa agtcaagctg acagcagcaa gttcattttc tttgttacgc aagatagtga
    14101 cggattgttc gtccacgcct gtttcaaaag gaaccattgt agaggcaact acttcaggtt
    14161 gagagctcaa aaagaaacga gcgaaagata cagcatacgt accgatatcc aacagagctc
    14221 ctccagctaa ttctggattg aagaaccggt ttttagggtc tggttctttg taactgccga
    14281 aaggtgcttg gatcattttt aaagccccca gttttcctgt atccattatt gagcgtaact
    14341 gctgataaag tggcatgttg aagattgtca tcgcttctgc taagatcaaa ttcttttctt
    14401 ctgctaagcg catagcgtca gccagttctt ttttattcat cgtgatggct ttttcacata
    14461 gaacgtgttt gcctgcttcc aatgctgcca agatatggtc gatatgctgt ctatttggta
    14521 cagcaatgta cacgatgtcg atttcagcat cgtctaacat ttcttgatag cttccgtaag
    14581 cttttggaat attgtaacgg tgagcgaagt tttccgcttt ttctgaagtt ctggaagcaa
    14641 ctgctgttag ttcgctcgtc tcttgattga agctttcggc aaactcatgt gcgatggtac
    14701 caagaccaac gattccccat tgatattttt tcatctgaaa acgctccttc atagttggat
    14761 tgagtttctt gtctgtctat tattataaca aaaatcacga agaataaaga ggagaggact
    14821 tgaaacatta aaaaaaatga aagcagcgat ttaagaggtg ctttttgatc aaaacttaag
    14881 tagaatagtt aaaaggaggg gataaacgtg catcaagtat atattgatct tattatgagt
    14941 gtattattag aacagtttga aactgaaaaa aattttttag atgaatactt gctgatcagt
    15001 ccagaacaat ggcaaaaatg gaaagatggc gagaaaaatc tgccatctga agcgatgcag
    15061 aaagtaaaaa atcttttcac tgattatgaa tggatgctaa cgcaaaagat tttgcgtcaa
    15121 acgattattt ttcccgaaaa acgaaacgtt gccgttgcag agtacaagca aatcaaaaca
    15181 cgcatcgcgc aaaaatggct tagcagcaaa gcagcaaaag tcgagctgat ccctttgaaa
    15241 aataatcagc aggctggtag ataccttgac ttgaaagtaa gtattcaata tgatgaatgg
    15301 ggctatgatg atattttgaa ttttcgtctg ccagcagatg ttcaaaaaca gatcgaagga
    15361 gaaaaaatcg aacttctcga ttgggtgaat gaaaatctgg aagaaactta cgttcatgag
    15421 gcgaatgggg aacgtgaaga acaacgaaag gatttttcaa gtgaataaat ttttaggatt
    15481 tggagcagtt gtgattatga tcgcagcaat gggctatgtt atttttagcg atgatgattc
    15541 gcatgttcaa gccggcaatc aagcagtcga acgaacaagc accaaaacaa ctgctagtaa
    15601 agaaaagaac agtcaaacaa ccagcagcaa ggtaaaggac aaagaaacaa aagatcgtac
    15661 gtccaaacaa ttgccagatg ttaaaacaga cgattggaag ctagttttgg ttggtccaga
    15721 tcacaaatta gaaaaagaaa ttgatgagtc tactcaattg gcatcattaa gtaacggata
    15781 cttgatcgac aaacgcattg ctccagaata tgaagcattt gccaaggctg ctgaagcagc
    15841 tggatttcca ttagtgatgg tctcagctta ccggtctgta tcatcccaac aacaagtttt
    15901 cgaacaaaat gtccaagatg tgatgagtcg tcaaggtgta tcagaagagg aggcgacaaa
    15961 gattactaaa gaaacgatta cggagccagg atacagcgaa caccacacag gtctggcagt
    16021 cgatgtcgtc gatcaaaact ggtacaacag ttatccttct actgtattgg atgcaagtta
    16081 cggagatcag cctggagcta aatggatcgc agataatgct gcgcagtatg gatttatcgt
    16141 tcgttatcca aaaggacacg aagatataac taagatcaca tatgaacctt ggcacttacg
    16201 ttatgtcggt aaagaaaacg ctgagtatat caccaaacac caattgacgc tagaagaata
    16261 tctgaatgaa ttaaatgaaa agtaggaatt gatcatggcg aaaaaaaagt ggaaaaagca
    16321 aaagcgcaag aatatccgtt ggccagcagt ggttgccggt gtagcagtga tactcttagc
    16381 ctttgttttt tccttgaaga atctgtcttc tccttttact gataatcagc aagaaaatga
    16441 agcgcaatcc catcagcaat ttattgaacg gcttgttccg catgcccaag aactacaaga
    16501 tggttatgga gtgcttccta gtatcatttt aggacaagct atattagaat caaattgggg
    16561 aaaaagtacc ttggctagca aatacaataa tcttttcggg atcaaggctt atggggatca
    16621 gaagaaagta agcctggaaa caaaggaatt tgttaacgaa gaatggataa ctatccaagg
    16681 agacttcaaa gtgtatgatt catgggaaca gtccatggat gaccatacac aactgttcgt
    16741 ccaaggagta gattggaatc ctgcgctgta tgaaaaagtg attacggcca ccaactatca
    16801 ggaagccgca caagcattgc aagatgcagg ttatgcgaca gaccccggtt atgcgcaaaa
    16861 aatcatccag gtcatcgaga cgtatcaatt gaatcagtat gaccggagat agcaaaggag
    16921 aagtggctga tggcagaaca atttacgcca gaaatcatga cggaattgag aaaagatatc
    16981 gtaagagtac cggaaatcat tttagaagca agcgggatca agatatttgg aaaattgatc
    17041 cgttcgatca tctttactac ggatattgcg atcatacgta atacaaatgc gaatgcagta
    17101 attgctgtgt atccttttac acctcatcca gcgattacta aaagcattat tgaggcagct
    17161 gacattccag ttttctccgg tgtaggtggt ggtttgacac aaggtgctcg ttcttcctac
    17221 atgagcatgt tcgcagaagc gcaaggttcg ttaggggtcg ttttgaacgg tcctacgcct
    17281 ttagagacag taaaagaagt ttgtcgagta gttgatattc cagttatcag tacggtaacg
    17341 tcgaaatata cagaaataga tgaaaagtta gcagctggta tttcgattat caatatcagt
    17401 gcaggtaaag aaactgcaga agcagtacgc cattttcgta aaaaatatcc tgaacttcct
    17461 atcattgcga caggtggtcc aacggaagaa agtattgctg agaccattca agcaggagca
    17521 aatgccatca catatacgcc accaagtagc ggagagcttt ttagtcgtaa aatggaaaaa
    17581 tatcgggaaa ctgagaaaaa gaatcatgaa taacaggtga gaatcaaaag acgaggaaga
    17641 gcagatagtg gctcttcctc gtcttttgta ctccagtact atttgttcag aattcaaaaa
    17701 aaagagaaat ttttttgttt ttttacctgt aatgtacata agaagagtta aaaccttttg
    17761 ttattattag cattctaaca aaaaaatgtc ttgatttatt gaagcgcttt ctatagaatg
    17821 aaaaacgaat aaaaactaaa aaaggcaccc gacaatttag tagcagctaa attgtcgggg
    17881 cttgaaaagt cagcgggaac tgaccaatca agttgccata gtcaatgctt gtgcatcgac
    17941 gtgtatattg tacctaattt catacagaat tactagttat ttttctgttt tttagggaag
    18001 agcgcacacg aagaagcatg cttatttagc tatgtcaagg atattgggca gttgtctgat
    18061 atcctttttt ctttgcgtaa ttatcctccc atcattgaag caagaagtgt ttttagcgat
    18121 gaaagaagaa tcagcactat ctaatttttc ttgaaagtat tgagttgttc atcctaggca
    18181 gaacgataag gcaagtcttg gtttttattc cccttgaaaa agatcgttta ataaatatgt
    18241 tctgtataaa aatacagtat attgaaaaat aacaatggaa gtagaggtgc agagaatgaa
    18301 gaaaagaatg agaatcatct tagctttaag tgttatgtta ttcttcttga cggcttgtgg
    18361 gcaagaagat gacaaaaaaa tcacgctgaa catggcttgg tggggttcgc aagtccgaca
    18421 cgatgcaaca gtaaaagtaa tcgagcttta tgaagaaaaa aatccccatg tgacgatcga
    18481 ttatgaattt tatgattatg aaggatatta cacaaaatta ggtacactag cagcatcaga
    18541 tgatatctgg gacattttcc aactgaacaa tcaatttcct caatacatca atcagattga
    18601 accattagat gaatatgtgg agcaagggat aattgatatt tctaatgcag atgaatccta
    18661 tttagcaacg acgacttacg acgggcaatt agttggactg tctaacgggg tgaattcgtt
    18721 tgcgattgct tacaataaaa atatatttga acaattaaat atccctgaac cagcttcaaa
    18781 ttggacatgg gatgaatttg aacaaatcag ctatcaaatc actgaagaat taggaatcta
    18841 tgctatttca agattcgaag attttatagc tggctgtatt attcaaatac cacaagttga
    18901 gaaaggattg aatttttata atcgggaaga tctatctaac agcttgggat ttgaaaatcc
    18961 agactatcta acagattttt tccagctgcg taaaaatctg gtagacaaag gggcccatcc
    19021 agaaccaggt ggtggagctg caagtacgac ggatctgtct tttcaggcgg ttagggatag
    19081 agaagcggca atggtgtttc tatcaagtaa ccagttgaca gaaatattgt caggggctcc
    19141 tgaagatatg gagattcagt tgattaatcc acctcgcaga aaagcagatg gagaatctgg
    19201 tatcccttta cgttcttcgc aaatgctgag tatggcaaaa agctcagccc ataaagaaga
    19261 agctgctaag tttattgatt tctttcaaaa cagtgaagaa gccaatgaaa ttttaaaagg
    19321 agaacgtggt gtaccaatca tgtcgaatat tcgagaacag ctcgctcgtg cagatgatgc
    19381 aactttgcaa gcgacatttg attatttaga aatgatttcc gatatggaca acggcgaagt
    19441 gaatgtcttt gatagtgctt tgaatccgga aattcaagac caatacaatc ttttagttga
    19501 aaaagtcatt tatgaagaag taacagcaga agaagcagca gagagtcttt atgagtttgc
    19561 gtcaaacatt ctaggatagc agcaggagga aggcatgaaa aaagaaaaac taaaacggtt
    19621 cttggatcaa gacaatgtgg tcggttacgt atttgcttct ccattcatcc taggatttct
    19681 attgataaca gtgataccga tgatcttgtc actttattat tcgttcaccg attatcgttt
    19741 gggaaatcca gtctcatgga tcggcttgga taattatata aagatgctga gtgatgcccg
    19801 ttttttgaac tccatccgag tcacttttct ctatgtattt gcatctgtac ctgtgaaact
    19861 gatctttggt ctattgattg cttatctttt aacgaggaat atcaaaggaa cgactttctt
    19921 tcgttctttg tattatgtgc ctagtttgat cggtggcagt atttctatcg ctctagtttg
    19981 gcgggagttg ttttctaaaa acgggttagt gaatattttc ctggaacgat taggcgcacc
    20041 tgcgctttcc tggtttagcg atcaaaaact ggtcatgatc cctcttgttt tgatgtctgc
    20101 ttggcaattc ggttcggcaa tgatcatctt tgctgcaggt atcaaagaga tcccaggctc
    20161 gtattatgaa gcggcagaga tcgatggagc gaacaaatgg cagacgtttt acaagatcac
    20221 gttacctctt ctttcgccga tcattttgta taacttggtt atgcagacaa tcgcggcgtt
    20281 tatgactttt acgcaggcat ttatcattac ccaaggtggt ccgaatgatg ccacgaactt
    20341 ttatgctctt tatgtgtata accaagcttt ttcttttaat tctatggggt atgctagtgc
    20401 tatttcgtgg atcatgctgt tgattatggg agctgtcacc tttctgattt tcaaatcatc
    20461 aaaaaaatgg gtcgttgtgt gaatgagagg aggaagaaat gaataataca gtaacgaaat
    20521 ggtttcaata cggttttatt ttgctattag gtctagtcat gatttatccg gtcttatgga
    20581 tgatcgcagg ttcttttaaa acggatcaag agattatgag tggttcgctg aatttgattc
    20641 cagagacttt tcaatgggaa aattacgcaa aaggatgggc ggggtttgct ggaatttcat
    20701 tttttacttt ctttaggaat tcgtttgtca tctctgcagt ttcgacgata gggacagttc
    20761 tgagctcgac ttgtattgct tacgcattat cgagaatcga tttcaaagga aaaagattct
    20821 ggttcgtcgt catgttagta acgatgatga ttcctgcaca agtgattttg attccgcagt
    20881 ttattattta caatcggctg aatttggttg ggacgtatgt gccgctgatt ttaccacatt
    20941 tttttggaca agcatttttc atttaccaga tcatgcaatt catggtcagc atccccaaag
    21001 agttagatga atcagcgatt attgacggat gcagcaagta tagtgtcttt accaaaatta
    21061 tttttccttt gctgaaacct tcgatcatca cgacgatcat catccagttt tattggaaat
    21121 gggacgactt catgggacca ttgatttatt taaacaagcc gagatcttac acggtatcga
    21181 ttgccatcaa attatttgca gacgctggat ctagtacgac ttacgcgagt atgtttgcga
    21241 tgtctacgtt gtcgctgcta ccggtttttc tgattttctt attcttcaat aaatatcttg
    21301 tccaggggat cagtacaagc ggtctgaagg gctgaatatg aaaaagccaa tagaagagat
    21361 gtttctttac aagctcaact actatgtctg tcggattttt ttaaccagtc ttatcagtaa
    21421 tgctcttttc gctttagttc taggatgttt gttcacttta ggattagtgc tgtctgctcg
    21481 tgttgtctat ctgatttatt ttttgatgat gtatccttct tattatctat ctgtccatta
    21541 cttggcagaa aaaaaggtat gttggaggaa aggaaaaatc aaacggatgt gtttcatcat
    21601 tacgttgttt cagctgtttg tcattatttt tgcagttgat tggtatttat tagtgggaaa
    21661 gattggagct tggtatctga ttccttttct attgtgtctg ttgctttttg tatcccatag
    21721 tttttttagt acgctaattg atatctgcaa agaagataaa gcagatagtc ttgtttattt
    21781 gatcaaaagg aatatctggc tgttgtcaaa aaacgtcaag gggtttctgt ggtatttatg
    21841 ccttttgtat ggactgttct ggtgtataca gcatgatttt ttactcactg cattactgta
    21901 tccagggctg ccatttgtca cagtattttg cttaaaagaa aaggagaatg actttaaatg
    21961 aagaaaaaat atgtacaagt cggacttggt gggagagcgc gtttttttta tgaggcaatt
    22021 gcctctacgt atcatcaaac ttgtgaacta gtcggttttt gtgatgtatc tcaaacaagg
    22081 atggattatg caaatcggat acttgtgaac aagtatggac agaaaaaagt aaagacatgt
    22141 atgccatcac aatttgagca actactggat gacgtacggc cggctgaagt cattattacg
    22201 tcagtagatc gaacgcatca tgattatatc attcgtgcaa tgaaaaaagg atatgatgtt
    22261 atttgcgaaa aacctctgac tatcgatgag gaaaaagctc aagaaataat aacggtccaa
    22321 aaacagacgg gaagaagcct gcgagtcact tttaattatc gttatgcacc gtatcacacg
    22381 aagataaagg aactgataga aagtgatgtg attggtgatg tgttttctat ccacttcgaa
    22441 tggctgttaa atacgcaaca cggggcagat tatttcagaa gatggcatcg aaataagcac
    22501 aatagcggcg ggcttcttgt tcataaatct acccatcatt ttgatctagt gaatttttgg
    22561 ttgaacagcc gtccccaaaa ggtatttgca ttcggtggat taaactttta tgggatgcat
    22621 aatgcggaga aacgcggagt agaaatattt tacagtcgat gctatggaag cgaactagct
    22681 aaagctgatc catttgctat agacctgtca gccaatgagg aattgcgaga aatgtattta
    22741 gaagcagaaa aagacagtgg atatattcgt gaccgcagtg tattttccga tgatatctct
    22801 attgaagata cactaggcgt aacggtcaaa tatgaaactg gtgcgatttt gacgtattcg
    22861 ctaaacgcgt atatgccgtg ggaaggattc aatgtttctt tcaatggcag caaaggacgg
    22921 atcgaaatga aagtgatcga aaagtcttac atcaatgcag gtggagataa aaaggatgaa
    22981 ggagcagccg ttcttcagtc catccatgtc tatccgatgt tcggtgagcc atatgaggta
    23041 atcgtcgaac aagaagcggg cggacatggc ggaggagatc cgcgtttatt agatgatcta
    23101 tttggtgaag aaaaagaaga agatccgtgg aatcgggcag caactcacgt agatggtatt
    23161 ttgtcgattt tgacaggaat cgcagcaaat cattctattg cttcaggaaa agcagtagtg
    23221 attgacgagc ttgtcagctt aaaagaataa gaatagattt acggaaatta cttcccgtag
    23281 tttataaaat cttgccttat tgccgatagg ctgtcttttg aacactattt attcggaaaa
    23341 aggggctatg acatgctttt tgtcatgcct tctttttatg tgggctgctt ttttagatag
    23401 gagaaaataa aaataactat ttaaacccga acaaaaagga ataatgcatt aaaaatcatt
    23461 agattttaca aaaaaataag taaaccacgt tgtcagtttc agaaagtagt gttagactgt
    23521 tttcaatatt tgaaacaaca ttttttattc aaatatggca aactgccttc acgtgcaaaa
    23581 cagttgcaag aaaatgaatt tacactatta ttagttttac atacctactt atatatcgtc
    23641 ataatatgga tgacagtttc tagccagtac cgtaaatgct ggactataag taaaagattg
    23701 gctatttcac ccttcttttc ttatgataaa gtggagggtg tttttcttta acagaaacat
    23761 agtaggtttt tgcacaaaag cggttatttc ggtattaata aatgataaag gagcaaataa
    23821 agtgaaagaa ttagttgaac gtattcaaaa agacggacgt atattaggcg agggcgtatt
    23881 aaaagtcgac agcttcgtca ctcaccaagt agaccctgta ttgatggaac aaatgggaaa
    23941 acggtttgca gaagtattta aagaacaagg aatcacgaaa gttgtgacca ttgaagcttc
    24001 tgggatcgca ccagctttat ttgctgcaag agaattagat gttccaatga tcttcgctag
    24061 aaaagcaaaa agtttgacga tggatgaaga attattgaca gcttctgttt attcttttac
    24121 gaaacaagtg acaagtacga tttctatttc tcgtaaattt ttatcttcag aagacaaagt
    24181 attgattatc gacgatttct tggcaaacgg acaagcggca aaaggattga tcgaattatg
    24241 ccagcaagct ggagcgcaag tagaaggaat cgggatcgtc atcgaaaagt ctttccaaga
    24301 tggacgtcag ttacttgaag aaatgggatt gagagtcgtt tctcttgcgc gtattgcttc
    24361 cttgacgaat ggacaagttg agtttcttga ggaggacgca taatcgtgga aaaagaaaca
    24421 caaaatggaa aagctgcggt tttagggctg cagcatctgc tagcgatgta tgcaggagca
    24481 gtagcggtgc ctcttttgat cggtacagga ctaggcttta atcaagaaca gatgacttat
    24541 ttgatttcaa ttgatatctt tatgtgtgga ttagcgacat tattgcaatt gacagttaac
    24601 cgatttttcg gaattggctt gcctgtcgtt ttaggatgtg cgatccaagc agtagctcca
    24661 ctgatcttga tcggaaccaa tgaaggagta ggagctatct acggatctat tatcgcttct
    24721 gggatctttg ttgtgttagt ttctggtgtc ttttctaaaa ttaaaaaact attccctcct
    24781 atagtaacag gaacggttat caccgttatt ggtttgactt tgattcctgt agcaatcgag
    24841 aaaatgggtg gaggtaatgc aacagcagct gattttggtg acaaaacaaa tctgctactt
    24901 gcatttgtga cgattctcct aattgtaggc gtgcaaatgc tggcaaaagg gttcattcgt
    24961 tcaatcgcgg tattgattgg tttagtcgga ggaagcatct tagctgcttt catgggaatg
    25021 attgatctga gcgcgattgg agatgcgccg ttgttccatg ttccacaacc cttttatttt
    25081 ggtaaaccaa ctttcgacgt atggtcgatt ttactaatga tcatcatttc catggtcagc
    25141 atggtagaat caacaggtgt atattttgca cttggtgaca ttactgggaa aaatgttgga
    25201 gaagaagaat tgaaaaaggg atatcgtgca gaaggacttg ccgttatctt aggcggtatt
    25261 ttcaatacat tcccttatac tggcttttct caaaatgtcg gattggttca actttcggga
    25321 atcaaaacaa gaaaacccat ctatttctct gcattcttct tgattgtttt aggattattc
    25381 cctaaaatcg gtgcgttagc acaaatcatc cctgagccgg tacttggtgg aggaatgtta
    25441 gtcatgttcg ggatggtggc agtccaagga atccgtatgc tgttggaagt tgatttcagc
    25501 aatgataaaa atctattgat tgttgctgtt tctgttggat ttgggctggg tttcaatgtt
    25561 acgccgacat tattcagtca aatgccgcaa acattgcaaa tgttcactgg aaatggcatc
    25621 gtaatgagta gcattacggc tatcatattg aatttgatct ttaatggatt gaaaaaagat
    25681 gaagaaatat tggaagaagc cttcaagaag taatagagca aaagaaaggt tgtgaaatca
    25741 gcgttttgac ctgattgtta gagaaacaaa cggaaaaata gttcctcgta ttttgccgtt
    25801 tgtttgagct aataccgagg gtcagctgat tgaacaccgt tgaaggaaga ggtcgtgagc
    25861 ctgccgttca tctccgagtc acaaggaaca atttcaaaaa aaagcttctc atgtttgttg
    25921 aatattgttc cttgtgaccg aggagttggc aggagaacac cgtttatcca taaaaaggaa
    25981 tgtgacctaa cttttgtcac attccttttt tttatcttta ataaacacga acaataaaat
    26041 gtttttcttt gttaacattc gaaatttctt tgacatcaaa aaaagtgatt gttaaaatat
    26101 gaatgtagaa aaactgggag gaaaaacaat gacagcaaaa atctctgtga ttatggggag
    26161 tacttcagat tgggaaacaa tgaaagaagc atgtcaaata ttggatgaat tcggtgtgtc
    26221 ttatgaaaaa aaggtcgttt ctgctcatcg aaccccagat ttgatgtttg aatatgcaca
    26281 aaacgctcga aaaaaaggaa tcaaaatcat tattgcagga gcagggggag ccgcgcatct
    26341 gccgggtatg gtcgcatcaa aaacaacttt gccggttatc ggtgtaccgg tccaatcacg
    26401 aacattaaat ggtttggatt cgcttctttc gattgtccaa atgcctggag gagtacctgt
    26461 agctactaca gcaatcggaa aagcgggagc aattaatgct ggactacttg cgatacagat
    26521 gctttccatg tatgatttag aattggaggc taaattggct gaaagacgtt caatgttggc
    26581 taaaactgtt atagaaagta gtgatcaact tggttaaacc tttgttgcct ggttcaacga
    26641 tcgggatcgt cggtggcggt caattaggtc gtatgatggc gattagtgca aaagaaatgg
    26701 gctttaaagt aggtgtgctg gatcctgtga gcgattgtcc agccgcacaa gttgcagatt
    26761 ggcacattga aagtgtctat gatgacacct ttgcgctgaa agagcttgcc agaagaacag
    26821 atgtcattac ctatgaattc gaaaacgtca gtatagaggc gttaaatgcg attttaccca
    26881 tgtcatttat tcctcaaggg acagacttgc ttgcgattac gcaagaccgc ctgttagaaa
    26941 aatctttttt ggaaacgaat aacatcgtga ttgctcctta tgcaacgatc gtcagtccga
    27001 cggatatcca agatgcgatt gacgggatcg gctatccttg tgttttgaaa accactcgag
    27061 gcggctatga tggcaagggg cagtatgttt tgaaaagtcg ggcggatcta gctcctgcca
    27121 tggacctttt aagagaagga acctgtgttt tagaagcatg gatcccgttt gaaaaagaga
    27181 tttcgatcat ggtggcagga aatggtcaag gagatttcac gacttttccg gtcgtggaaa
    27241 acatccacca caacaatatt cttcatgaaa cgattgctcc agccgcgatt gatcaggatg
    27301 tgatcgagga ggctgagcgt attgcccgtg tgattgcaga agcagtgagc ctttcaggtg
    27361 ttttaggggt agaaatgttt ttaacaaaaa caggcgggct atatgtgaat gaattagctc
    27421 ctcgtcctca caattctgga cattattcta ttgaagcttg ttctatgagt cagttcgatg
    27481 cccatattcg aggtatttgt ggttggccat taggagaaaa agtacgtttg ataagcaatg
    27541 cagtaatggt caatgttttg ggagaacaac tgctggaaag ctatcgcttg attgagaata
    27601 agccagactg gcaattccat ttctatggaa aaaaacaagc aaaaaaaggt cgcaagatgg
    27661 gacatatcac tatcttgaca gaagacatcc agcaaacgct ggaagaaatc aaacaaacga
    27721 atatttggga ttagaggaga aaaaacatgt tagaacgtta tacacgccct gaaatgggga
    27781 atatctggac ggaagagaac aaataccgtg cgtggctcga agtcgagatt ttagcagatg
    27841 aagcttgggc agaacttggt gaaatcccgc aagaagatgt gaaaaaaatt cgtgagcatg
    27901 catcatttga tatcgatcgg atcttggaaa tcgaaaaatt aacacgccat gatgtcgttg
    27961 cgtttacacg agcggtttct gaaacattgg gagaagaaag aaaatgggtc cattatggtc
    28021 taacaagtac ggatgtcgta gatactgctt atggctatct catcaagcaa gcaaatgata
    28081 ttttacggga agacttgaaa cgttttacgg aaatcattgg cgaaaaagca aaagaacaca
    28141 aatatacagt gatgatgggt cggacacacg gtgtccatgc tgaaccaact acttttggat
    28201 tgaaacttgc cacatggtat tcagaaatga aacggaacat cgaacgattt gaacatgcgg
    28261 caaaaggagt agaagcagga aaaatctcag gagcagtggg aacgttcgca aatatcccgc
    28321 catttattga agagtatgta tgtaaacaat tggggatccg tccacaagaa atttctactc
    28381 aagttctccc tcgggatcta catgctgaat atttctcagc gatggcgctt attgctacaa
    28441 gtattgaacg ttttgcaaca gagatacgcg gattgcaaaa atccgaaaca cgtgaagtag
    28501 aagaattttt tgcgaaaggt caaaaaggtt cttctgctat gccgcacaaa aggaatccga
    28561 tcggttcaga aaatatggct ggtctagcca gagtggtgcg cggacacatg gtcactgcct
    28621 atgaaaatgt cacattatgg catgaaagag atatttctca ttcttcagca gaacgaatca
    28681 tcttgcctga taccacgatt ttattgaatt acatgttaaa tcgttttggc aatatcgtta
    28741 aaaatctaac ggtctttcct gaaaacatga agcggaatat gtctgcaaca tttggtttga
    28801 tttatagcca gcgcgttatg ctgaaattga tcgataaggg aatgacaaga gaagaagctt
    28861 atgatcttgt tcagccaaaa actgcgtacg cttgggacaa ccaagtgcag ttccgcccgc
    28921 tgttagaaga agatccaacc atcacatcgg tattatctaa agaagaaata gacgatgcct
    28981 ttgattatca tcatcatttg aaaaacgttg acgtgatatt caaacgagta ggtttggaat
    29041 aaagaagagt gagttgcaaa attcaattaa aatagaatag caaaaataac caaaaaagaa
    29101 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
    29161 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
    29221 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
    29281 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
    29341 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
    29401 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
    29461 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
    29521 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
    29581 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
    29641 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
    29701 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
    29761 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
    29821 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
    29881 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
    29941 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
    30001 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
    30061 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
    30121 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
    30181 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
    30241 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
    30301 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
    30361 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
    30421 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
    30481 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
    30541 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
    30601 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
    30661 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
    30721 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
    30781 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
    30841 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
    30901 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
    30961 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
    31021 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
    31081 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
    31141 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
    31201 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
    31261 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
    31321 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
    31381 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
    31441 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
    31501 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
    31561 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
    31621 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
    31681 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
    31741 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
    31801 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
    31861 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
    31921 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
    31981 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
    32041 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
    32101 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
    32161 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
    32221 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
    32281 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
    32341 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
    32401 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
    32461 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
    32521 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
    32581 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
    32641 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
    32701 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
    32761 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
    32821 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
    32881 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
    32941 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
    33001 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
    33061 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
    33121 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
    33181 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
    33241 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
    33301 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
    33361 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
    33421 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
    33481 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
    33541 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
    33601 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
    33661 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
    33721 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
    33781 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
    33841 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
    33901 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
    33961 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
    34021 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
    34081 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
    34141 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
    34201 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
    34261 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
    34321 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
    34381 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
    34441 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
    34501 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
    34561 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
    34621 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
    34681 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
    34741 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
    34801 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
    34861 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
    34921 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
    34981 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
    35041 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
    35101 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
    35161 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
    35221 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
    35281 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
    35341 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
    35401 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
    35461 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
    35521 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
    35581 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
    35641 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
    35701 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
    35761 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
    35821 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
    35881 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
    35941 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
    36001 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
    36061 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcg aagaatcgaa
    36121 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
    36181 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
    36241 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
    36301 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
    36361 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
    36421 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
    36481 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
    36541 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
    36601 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
    36661 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
    36721 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
    36781 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
    36841 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
    36901 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
    36961 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
    37021 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
    37081 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
    37141 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
    37201 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
    37261 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
    37321 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
    37381 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
    37441 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
    37501 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
    37561 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
    37621 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
    37681 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
    37741 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
    37801 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
    37861 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
    37921 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
    37981 agccgactcc agcagaagca aaagcattag tgtttgcttg gagagctgtc aaacatgtga
    38041 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
    38101 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
    38161 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
    38221 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
    38281 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
    38341 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
    38401 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
    38461 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
    38521 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
    38581 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
    38641 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
    38701 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
    38761 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
    38821 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
    38881 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
    38941 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
    39001 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
    39061 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    39121 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    39181 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    39241 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    39301 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    39361 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    39421 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    39481 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    39541 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    39601 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    39661 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    39721 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    39781 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    39841 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    39901 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    39961 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    40021 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    40081 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    40141 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    40201 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    40261 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    40321 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    40381 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    40441 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    40501 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    40561 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    40621 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    40681 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    40741 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    40801 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    40861 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    40921 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    40981 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    41041 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    41101 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    41161 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    41221 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    41281 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    41341 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    41401 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    41461 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    41521 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    41581 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    41641 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    41701 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    41761 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    41821 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    41881 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    41941 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    42001 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    42061 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    42121 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    42181 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    42241 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    42301 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    42361 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    42421 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    42481 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    42541 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    42601 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    42661 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    42721 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    42781 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    42841 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    42901 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    42961 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    43021 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    43081 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    43141 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    43201 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    43261 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    43321 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    43381 gattgatgat ctcttcaatg gtatccataa aatcttttgc caagatatta ttgatcagct
    43441 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    43501 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    43561 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    43621 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    43681 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    43741 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    43801 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    43861 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    43921 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    43981 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    44041 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    44101 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    44161 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    44221 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    44281 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    44341 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    44401 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    44461 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    44521 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    44581 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    44641 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    44701 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    44761 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    44821 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    44881 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    44941 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    45001 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    45061 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    45121 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    45181 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    45241 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    45301 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    45361 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    45421 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    45481 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    45541 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    45601 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    45661 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    45721 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    45781 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    45841 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    45901 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    45961 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    46021 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    46081 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    46141 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    46201 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    46261 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    46321 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    46381 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    46441 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    46501 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    46561 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    46621 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    46681 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    46741 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    46801 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    46861 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    46921 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    46981 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    47041 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    47101 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    47161 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    47221 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    47281 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    47341 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    47401 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    47461 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    47521 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    47581 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    47641 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    47701 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    47761 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    47821 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    47881 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    47941 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    48001 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    48061 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    48121 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    48181 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    48241 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    48301 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    48361 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    48421 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    48481 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    48541 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    48601 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    48661 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    48721 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    48781 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    48841 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    48901 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    48961 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    49021 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    49081 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    49141 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    49201 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    49261 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    49321 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    49381 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    49441 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    49501 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    49561 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    49621 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    49681 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    49741 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    49801 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    49861 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    49921 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    49981 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    50041 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    50101 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    50161 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    50221 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    50281 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    50341 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    50401 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    50461 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    50521 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    50581 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    50641 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    50701 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    50761 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    50821 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    50881 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    50941 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    51001 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    51061 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    51121 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    51181 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    51241 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    51301 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    51361 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    51421 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    51481 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    51541 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    51601 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    51661 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    51721 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    51781 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    51841 ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct tggatttaaa
    51901 tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact aagaaaaaac
    51961 cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta
    52021 taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa
    52081 atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat gctgttaata
    52141 tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca
    52201 taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt
    52261 ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca
    52321 aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca
    52381 gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta
    52441 tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa
    52501 cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa
    52561 gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt
    52621 tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg
    52681 tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa
    52741 attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga
    52801 aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt
    52861 tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac
    52921 aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa
    52981 aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta
    53041 tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc
    53101 gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac
    53161 cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa
    53221 tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact
    53281 aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca
    53341 gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt
    53401 tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag
    53461 atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg
    53521 ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc
    53581 gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt
    53641 tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg
    53701 acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac
    53761 ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata
    53821 atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt
    53881 atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag
    53941 aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc
    54001 ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag
    54061 caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg
    54121 aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg
    54181 aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc
    54241 aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag
    54301 tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg
    54361 caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa
    54421 acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc gcagaaggtg
    54481 aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag
    54541 aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga acggttaaaa
    54601 atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta gaaatcgatg
    54661 caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc caaactccag
    54721 ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca agcgaaggag
    54781 tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat
    54841 ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa gttcaaaatg
    54901 acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac gtgattgttc
    54961 cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct cctggacaca
    55021 atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat
    55081 caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg gctatgccat
    55141 ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact gcaactggaa
    55201 aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg
    55261 caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct cctgcagcag
    55321 aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac ttggaagaac
    55381 gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac agtaagcata
    55441 cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg gataatcgga
    55501 aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg ccatatgtcg
    55561 tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct atcgacgatg
    55621 caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact gatacggatc
    55681 acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt gctattgcag
    55741 acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca gaagatatgc
    55801 gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg tttacaccag
    55861 tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa caaccaatcg
    55921 taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc
    55981 accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa cgtcttttag
    56041 ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg agattttgct
    56101 gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc tgccatccgc
    56161 gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg cggtgtttgt
    56221 ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca ttatcaagag
    56281 tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa
    56341 acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt tggtttccta
    56401 ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca
    56461 ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc aatcatcgca
    56521 acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt gttagattct
    56581 actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg tggcggtgtg
    56641 attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac gattcttgaa
    56701 ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt agaagatgat
    56761 ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac
    56821 ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt tactgctgat
    56881 tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt agaacaagcg
    56941 ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg tacgaacgta
    57001 cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca caaagccagt
    57061 tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt agattataaa
    57121 gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat gacgatcaaa
    57181 gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc agggaacggt
    57241 cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac gatcgaagat
    57301 aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta
    57361 gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat tcatcctcat
    57421 ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt accaatccat
    57481 atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta
    57541 gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg gtttattacc
    57601 acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg caagaatatc
    57661 agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg aagatgtggg
    57721 aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg aagacttatc
    57781 agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga cgggaatttg
    57841 aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca
    57901 aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata tcaagtcctg
    57961 gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa
    58021 atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt ttctaatcga
    58081 aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag ggtgtaatca
    58141 attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca ccttgaattt
    58201 tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg gcgttggaaa
    58261 gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa aaggaatagg
    58321 cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat cggaaggctt
    58381 acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag aaattgcgga
    58441 aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg caatcttgaa
    58501 aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag cgggaaatgt
    58561 cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt tgacatttga
    58621 aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg cagacctttt
    58681 gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa
    58741 agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa aagtctgcta
    58801 taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa ggagttagtt
    58861 acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga
    58921 aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc acatacgcaa
    58981 ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta
    59041 gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga tactccagga
    59101 cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg
    59161 gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa aaaagcactg
    59221 gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc tgctcgtcct
    59281 gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga tgatgatcag
    59341 ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt gtcagatgat
    59401 ccagcagatc aagaaccaac catggcacct atttttgata caattattga aaaaatccct
    59461 gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact tgactacaac
    59521 gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa agtaggagac
    59581 caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac gaaattattt
    59641 ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga cttgattgct
    59701 gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat
    59761 gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt agttaataat
    59821 tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga agaacgtttg
    59881 atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc tccagatgca
    59941 tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa catgcgccgt
    60001 gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat cgacggtgta
    60061 aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat gggtagtgtt
    60121 atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac aggaaacgga
    60181 caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc aactgaattc
    60241 ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata tctgccaatg
    60301 cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa
    60361 gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt
    60421 acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga tttgactgtc
    60481 aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga ccaaacatct
    60541 gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt gaatgatgat
    60601 gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt agagaaaaat
    60661 gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag agagaaacca
    60721 gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt ggagaagtca
    60781 tctttgtcaa tggacataaa ttcagaaaat acgccgaaag gcgtttaaaa gacttttaaa
    60841 aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga aaggagatca
    60901 acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg
    60961 acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa gaaattatcc
    61021 agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt gggaaatcaa
    61081 tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg tcccctttaa
    61141 tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc gccactttta
    61201 tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc gatagacagc
    61261 agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt atccaagcaa
    61321 tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc tctcagtggg
    61381 ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa ctgccaaatc
    61441 gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct gacataaaaa
    61501 gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga gaaaatctac
    61561 gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat ttaaagatca
    61621 acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac cgtctctatc
    61681 atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta aatgagaatg
    61741 agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt gtagcgacaa
    61801 atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat tatcaaattc
    61861 ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat ggtctttcca
    61921 gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt tttatccagc
    61981 agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa gcgatgacag
    62041 aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt ggcgagacaa
    62101 gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta gaagtaacga
    62161 cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa
    62221 tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta ggttttggac
    62281 atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg
    62341 aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct gttttgaaag
    62401 taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga aaggaaccca
    62461 agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg ctgcgggaac
    62521 tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt tcagattcca
    62581 cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt cagataaaag
    62641 gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg ataaaaaatg
    62701 cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa taaaaacaac
    62761 tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat taaggaatta
    62821 gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa
    62881 tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc tctgtatctc
    62941 acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt cccggcaagt
    63001 cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga tgttaagtga
    63061 gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt atgataaaaa
    63121 agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca tctattagac
    63181 tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat ggtatatagt
    63241 tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt cattaaccag
    63301 agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat gaaaacagat
    63361 gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat tttgctgctg
    63421 attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca aattgcagga
    63481 ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct gtctctgact
    63541 ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa acgtccactt
    63601 gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg taatgcggca
    63661 gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta tgtgtatact
    63721 ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct gatcaatatc
    63781 acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt tgctatttac
    63841 caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg ctactatgcc
    63901 atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa aaaaggattt
    63961 ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact gggagtgatt
    64021 atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt ccttgtaggt
    64081 atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc aatgtttttg
    64141 atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac agggatcacg
    64201 ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg tgtcggcttt
    64261 gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata aaaaaagatg
    64321 taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt
    64381 atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag aagaagatga
    64441 atattccgta caccgtttca aagcggatga agcatattta gtaggacaag gaaaaaaacc
    64501 gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat gcggagctga
    64561 cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga
    64621 agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt ttggagataa
    64681 aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag gcacagatgg
    64741 accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt atccgattat
    64801 gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg atgaaaagag
    64861 tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga
    64921 agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga ttctcgggga
    64981 tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc gccgtcacca
    65041 aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg
    65101 ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt
    65161 tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc aagtggaaca
    65221 tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt tgattgctca
    65281 aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta aattaaatgg
    65341 ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt taccagacac
    65401 agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg atgtcggaaa
    65461 tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa aagtatgtac
    65521 gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga aagaatttag
    65581 aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac atcctgaatt
    65641 ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat ttgaatttcc
    65701 tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg
    65761 ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga
    65821 tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga agggtgccga
    65881 tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata cgacatttag
    65941 agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca agcagattgc
    66001 acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg ggggagcaac
    66061 atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga gaaaaattcg
    66121 ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta
    66181 ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag cgcaaggaat
    66241 cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa aaagtatcca
    66301 agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg gcgatatcaa
    66361 cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa aggaactaga
    66421 acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta aaccgcaggc
    66481 agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc atctgcatac
    66541 tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag caggagtaga
    66601 catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta gcatgagcag
    66661 cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg aaaatgcaca
    66721 gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg aaaacggctt
    66781 gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt attcgaatct
    66841 acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca aacaaatgta
    66901 tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat ctaaagtagt
    66961 tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata tttatgaaaa
    67021 aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag aactagggca
    67081 acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca gacctgcatt
    67141 gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt
    67201 ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt tgatgtatcc
    67261 gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta cattactgga
    67321 cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg
    67381 cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg aagggaatcg
    67441 cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag ataattcgat
    67501 catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac aaattggagc
    67561 aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg
    67621 tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag cacgttttga
    67681 cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg gtgacctttt
    67741 gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag actgggtttg
    67801 ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt cttgtttccg
    67861 ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat gacaacgtgg
    67921 aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc gatcgatcag
    67981 ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg ttttgaaaca
    68041 agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca agacagcaga
    68101 gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg
    68161 atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac ttttgagtac
    68221 catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg tccgctaatt
    68281 gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa cggcctattt
    68341 tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca agtgtttggt
    68401 gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat caatcgcgtt
    68461 aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga acttcccata
    68521 tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga ttttttgaca
    68581 aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc gctaggcagt
    68641 gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt aatcagtaaa
    68701 caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc tactttttcg
    68761 tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc gggaacggat
    68821 tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga agaaaacgga
    68881 gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg atcaatgcct
    68941 ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg aacataaaca
    69001 gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga
    69061 agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg aaaaaaaacg
    69121 agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag ctttccgata
    69181 tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg ccgaagaagt
    69241 ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa aacactttgg
    69301 atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca aaacgacgcg
    69361 ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt
    69421 tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa gagacattgg
    69481 aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa cgttcttacc
    69541 gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta ttaaaagacg
    69601 aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat ctttaaaaat
    69661 ctcttatgag cagtttttaa ataaaaaata ccttggataa cgatcgattt ttgatcattg
    69721 tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga gtagatgcgg
    69781 ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga
    69841 ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata ttttgatgga
    69901 gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc agtatctaga
    69961 ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat catcaaagaa
    70021 aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga tgcggacaaa
    70081 gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct tgatccgcca
    70141 tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg acttttttct
    70201 aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga aactgtggct
    70261 ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat ctatagaaaa
    70321 gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt acaaaaggtc
    70381 acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt ggtgttttta
    70441 tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg atcacaaaag
    70501 ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa
    70561 cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc aaagactttg
    70621 aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg gaaactgtct
    70681 tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa gaagtactgc
    70741 actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct ttagctggaa
    70801 agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat catatttgga
    70861 atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc accgggagcc
    70921 acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg ttctgggtct
    70981 ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa
    71041 atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc gacaaatgaa
    71101 gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc aatcgaacaa
    71161 gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata tgtcatgagt
    71221 gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt aacaaaagta
    71281 gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag ccaaaaagtt
    71341 ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc ttctggtgat
    71401 ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac tgaccatacc
    71461 gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg gccatcagcg
    71521 ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt gcgacatggt
    71581 aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg aatcggagga
    71641 atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt tgcaccagat
    71701 gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa
    71761 gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta
    71821 caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa agaggggttg
    71881 tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa
    71941 acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag agctcactgt
    72001 ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc tttgaaaaaa
    72061 gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg atcatttgga
    72121 tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca aatttgctat
    72181 tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt agttccagtg
    72241 gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt
    72301 acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct tcagaagaac
    72361 gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat agcgatcgaa
    72421 tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct gtaggtgaag
    72481 agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct acagcagatt
    72541 ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag cagattcctg
    72601 ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac ggatccttcc
    72661 agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt gaaaaactaa
    72721 aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc aataaatgga
    72781 cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag gaggtagtat
    72841 ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc aatgctgaat
    72901 gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt cagcggcaat
    72961 aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac agcagataaa
    73021 gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc taaagaaacg
    73081 taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt tctgatcatg
    73141 ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg aataacaagt
    73201 tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat tgtgaagtat
    73261 cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata atgggctaat
    73321 ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg
    73381 ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg
    73441 gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag
    73501 ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg gctcaggcag
    73561 tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca ctcgttcgag
    73621 aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc cactgcatag
    73681 atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca gagatgaatg
    73741 cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc tatgtcacac
    73801 attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt
    73861 atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat
    73921 ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat gaattaacag
    73981 gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt tccggcaatt
    74041 acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg tttgcttctt
    74101 gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt
    74161 ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt tttagaggaa
    74221 aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa tggtaatttt
    74281 ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc ttatcaagta
    74341 aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat agaattgact
    74401 gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt tgaccaagaa
    74461 gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat tgaacagatc
    74521 accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt tatctccttt
    74581 gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt atggggctat
    74641 aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt gctgcatctg
    74701 ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta tttatttcga
    74761 cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac tatctgttat
    74821 gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga
    74881 ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc agttgtcttt
    74941 tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca gcttagtttt
    75001 ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa aaaaacggcc
    75061 tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt ttatgagtgg
    75121 cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt tctttttctt
    75181 cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt acttttgttg
    75241 atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg gtcgtctctc
    75301 attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt actgatttgt
    75361 atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct tttttctttt
    75421 atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat cgatgtcggt
    75481 caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt gatcgatact
    75541 ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg tcctcctgct
    75601 gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga taaactcatc
    75661 ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca gcattttact
    75721 atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca aaggcattta
    75781 tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga caggatcagc
    75841 ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag aaatgaagat
    75901 tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg agatttgaat
    75961 caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa
    76021 ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca tattgaagca
    76081 aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca cagcgaagta
    76141 ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca aggaatgatc
    76201 acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat gatagactag
    76261 taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt tgagtataca
    76321 agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga ttttaggaac
    76381 agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc aattagaggg
    76441 cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag atgaggtagt
    76501 agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg tagagaatcc
    76561 ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg atttattgac
    76621 ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc catatgaaaa
    76681 gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa acagcaatta tcatcaatgt
    76741 ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag agaattcctc
    76801 aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact tagatttatc
    76861 caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac agcaaatcac
    76921 ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt ttgacatgac
    76981 ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag atttagttat
    77041 gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac gtttattttt
    77101 acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg aaacattgaa
    77161 aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg atgaacattt
    77221 gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa cgggacaaat
    77281 cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt taagaaatcg
    77341 ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg
    77401 acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt gcttaaacga
    77461 gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc catatcaata
    77521 gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc tacagcatct
    77581 tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt cacattagcg
    77641 ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag tttcacctcc
    77701 gataacttaa gttcttacgc gtgttaccgc ataattcaac attcattatt atacctaaag
    77761 gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct tttattagga
    77821 ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg
    77881 gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa agtttgaatg
    77941 ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag ttttttttga
    78001 aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca gttattttat
    78061 aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt aatgtattca
    78121 tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt ccatttgcca
    78181 cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc caaatagcga
    78241 tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc agtcagcatc
    78301 attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt ttccacaagc
    78361 gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat tttgatattc
    78421 gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata acaaaaaaga
    78481 gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca ttggcacggc
    78541 ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc atcccctata
    78601 tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa atcaggaaat
    78661 aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc gattgttcct
    78721 atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat cacggatcag
    78781 taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc
    78841 aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg cggcgattcc
    78901 tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc
    78961 agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac cgcaaaaaac
    79021 agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac cgatttctcc
    79081 tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat tgattccttt
    79141 gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa tcaaaacaaa
    79201 agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta aaataattcc
    79261 taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca atcataacat
    79321 gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat aagctgtttt
    79381 tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga agacagccgc
    79441 gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc aggtaaaggt
    79501 cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg tcctacaact
    79561 ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag aataacaaga
    79621 atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag ccgatgactg
    79681 tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc catagcggca
    79741 tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg cgcatcattg
    79801 actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt gactagttgt
    79861 gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg ttcagcaatg
    79921 gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt ttgggattta
    79981 aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc taaaacagca
    80041 attaactgct gatctttaga aagataaatc acggttttgc cttgattttc caaacgttcg
    80101 atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc gtgcttgcca
    80161 actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat ttcttctatt
    80221 ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat cgcatgagca
    80281 agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa atccttttca
    80341 tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc tgttttatca
    80401 aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt gattaaaatc
    80461 ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga tgcgacaagc
    80521 gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc atcccaactc
    80581 caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa aatcaatttt
    80641 acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt attctgcgct
    80701 tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt tgttactcga
    80761 atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc taaatatttt
    80821 tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg tgtgctttct
    80881 aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt atccaccttt
    80941 agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg ggctttttgt
    81001 ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt gtattcctct
    81061 aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta attaccgatc
    81121 aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt ccgatcattc
    81181 cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat ggcactgata
    81241 taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa gatcataaat
    81301 aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt tattagacct
    81361 ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac agctaaatga
    81421 gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta tttataatga
    81481 ttattattaa agaaaataaa aaaccagaac atttctcaaa taagagaagt gttctggttt
    81541 tttctacatt gcaataaacc aataaccaat caaaatgata ccaaggataa tacgatacca
    81601 gccaaaagcg gtaaagtcgt ttttcttcaa atagcttagt aagaatttga tagctaagac
    81661 ggaaaccaca aaagatacga atgttcctac aagaagaaca acagtttctt ccattttgaa
    81721 tgaattccct tcagctaaat atttgacgat cttcaagccg cttgcgccaa acatgacagg
    81781 aatccctaag aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa tcaagataga
    81841 tcctaggatc gttgcgccag aacgggatgt tccgggaaca agtgccaaga cttggaataa
    81901 cccaacaaga agagctgctt ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt
    81961 gtttttgttg cgtttttcga caataataaa ggcaataccg taaacgatca aaacaatggc
    82021 aactggtaag aacttgtgga aatttgcttc taaccaatca tccaaaggca aaccaatgat
    82081 gatggctggt atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat cttttttttc
    82141 taccgttgat ttacttggag aaaatgggtt taacttgtga aaataaagaa cgacgacagc
    82201 caagatcgcc cctaattgga taacgacatt gaacatcgac ataaattgtg aactggcatc
    82261 cagtttgatg aattcatctg ctaggatcaa atgaccagta ctactgatgg gcagccattc
    82321 cgttattcct tcaataatac cgagaatcac tgctttgata atatttgcaa aaaacataaa
    82381 ttccttcctt cccaattaag atacatctta tagtataccg ttattttttg agaaacccaa
    82441 tctgtttttt aatattaaga aaaacttgag aaataaagca gataagtaca cgaagagaaa
    82501 aaagaagaca aagccctcgt tcagactttg ccttttatga ttctctggta aaaattaaaa
    82561 tatccccatt atagaaacga gtcgcgacta tgtgttttcc cgttatcgat ataagacgta
    82621 tcattgatgc cgtggataga aaatcgaccg tttgcataag tgacctttgt tacgctgcca
    82681 tttaaaagcc cagcacgaac aggctgttta ggatcgatca gattaagcaa aaaggcgatc
    82741 gtcaaaccat gagatacgac taaaacattt ccaccaccat ttttttcgca gtgatgggcg
    82801 atatcttcaa atcctgtcca tacacggtct cgcaatgttt cataagtttc cgcccaccca
    82861 gcggtatcta agttgtatac agcattagca atttgttcaa aagaaacttc tgtggtaaac
    82921 atttcatcat atgttttaaa attcaataca cgagggatca caccccacat ttccatgtca
    82981 tatccacctt caagagaccc gaaacaccat tctctaatcc ttttttcaat aaaataagga
    83041 atttcttttc ccttagaatg ttctcctaga ataatacggg cagtttcgat tgcacgacca
    83101 ctgtcactag aataagcaaa tgaaaagtcg acatttttta acccgcgacc taaataatga
    83161 atgccttctt gtccttgctt tgtgaggggt gtatcacacc agccttgtac tcgttcaatt
    83221 gtattgaaca tcggttttcc gtgtcgtacg atataaagtg tagtttctgt cattttattc
    83281 tcctcctaag tcgttttaac gattgaaaaa aggatttgtc cttttttcct gttcaatcgt
    83341 cgttgcatcg ccatgtcctg gatagactgc aaattcatca ggtaatacaa acagctgggt
    83401 ccgaatacta tgaagtaatt gttctaaatt ccctgtatac agatcggttc taccgatact
    83461 tcctctaaac agggcgtcgc cagaaacgac aaaatcatca aaaataaagc tgacgcttcc
    83521 tatagaatgc cctggggtag ggacgacgcg aaaagacatg tttcctaaag tataagtttt
    83581 catttcaaat tcatattctg caggcgagac aataacatca gcaatatcat catgtctacc
    83641 gagtccagaa agattcaaaa tcggatcacc tagccatttt tgctctagtg ggctgacata
    83701 aactgggatt tgataatacg cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt
    83761 atgggtaagc aaaatcgcta ccggctgttg gtttgttttt ttgatttctt gtttgattcg
    83821 ttccgcatct tctccagggt caatgatcaa aagtgcttct tcgttgtaga ccaaataaca
    83881 gttttcttca atcattcctg tttttaatct ttcgatatgc atgtgcattc ctccttgata
    83941 gccaaaactt ctttcatcca ttagtataac gcattcattg ctaggaaaca aaaacaggta
    84001 cttatttctc tttacttatc tgaattatca gactatatga aaagtatttg gatattctaa
    84061 agagaaggaa agtaagtttt catagcattt taatatgtcc ttgttacatt agattcatcc
    84121 aaaaatccaa attctatatc ctatatccct gcctaatcag cagggtgttt ttttatgttt
    84181 ggaaagagag ctctttttgc taaactaggt tttttcaggt aatcttagag agaaagaggt
    84241 ggtggaaatg atcaaacagt caaaaatatc tgtgcggcac ttagtagaat ttattttgcg
    84301 acgtggaagt atcgacaatc gtaaaaaaag taaccatact gcattagaag gagcgagaat
    84361 ccaccgcaag ctccaaaaag atgcgggaga aaactatgaa aaagaagtct ttttaaaaac
    84421 aacagttcaa ttagatgaat attacctgat cgttgaaggc cgtgcagacg gtattttcaa
    84481 aagagacggt atttattata tagatgaaat caaaacctcc gaaccacgat ttgaagattt
    84541 agaaccagaa caagtggaac ttttctttca tcaagcaagg gtgtatgcct atatctatag
    84601 tcaagaacat gatttacaag aaataaatct gcaattgact tacttccaga cggcagaaga
    84661 agtgatcacg agaaaggttg aacatcagac aagagagcag ttagaaactt tttttaaaaa
    84721 gctgactgaa gattataaag aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa
    84781 tgcctctttg atggctttga aattccccca tgatgagtat aggaaaggtc agcgtgaact
    84841 agcggtagct gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag aagcacctac
    84901 cggaacagga aaaacaatct ctacgttatt ccccgctttg aaagctgtag gggaagaaga
    84961 gggagaaaag atcttttatc taacggctaa aacaataact agacaagtag ctgaagatgc
    85021 tatgacagca ttaaaagata caggagctga agtaaaaagt gtcactttga cggcgaaaga
    85081 caagatctgc tttttagatg agacgacctg taatccagat cagtgcccat atgccaatgg
    85141 ttattacaat cggattaatg aaggtttatg ggatcttttg aatcatgaga atcagatcac
    85201 tcgtgaagtc atcgaaactt atgcaagaaa gcatacactt tgtcctttcg aactatcatt
    85261 agatgtcagt atctggtgtg acgtaatcat tggggattac aattatttat ttgatccaac
    85321 tgtttattta agacgttttt ttgaagatga aaaaaacgag gactatctgt ttttgattga
    85381 cgaagcacat aatttggtga atcgttcccg agagatgtat tcggcagaac tatcttatga
    85441 aaaaacgaaa cgaagtaagg aagctatacc aaaggaattc aaaaaacttc atcgccgttt
    85501 caacaaacta ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag atcattggga
    85561 ttatcatcat caaaaagcac cggctgaatc tttagtaaag gcaggttatc aattaagcga
    85621 aaaaattaaa gaatggctgg cagaatttcc tgagcaccca tcacaagaac aattgcttcc
    85681 ttattatttt gatttgcttc attttttgaa ggtcagtgaa ttttatgatg atcactacga
    85741 aacaacagtc gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta ttgatccaag
    85801 cttgtttctt gagcagtcgt tggataaagg aaaaagcagt attctttttt ctgctagttt
    85861 ttcaccactc tcttattatc aggaaacact ggggggacaa aaaagtctgg cttacaaact
    85921 gccgagtcct ttcccagaag gaaaccagca agttttcatc gcaaattacc tagagacgac
    85981 ttatcgaaaa agggaacaca gtctggcaaa attagtagag acgattcatg cttttgctga
    86041 tggaaaggtc gggaattatt tcgttttctt tccttcttat caatatttag atcttgcggt
    86101 ggaagcattc agacagcaat atcccgacag caatgtgcta atccaagaaa cggacatgaa
    86161 tgaggaagaa cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg aaacattgat
    86221 tggattttgc gtacttggcg ggattttttc agaaggaatc gatttgagag gggaccgatt
    86281 gatcggcagt atgatcgtag gtgtaggttt gccgcagatg aatcatgagc aggaactgat
    86341 caaatcatat tatgatgaga aggaacacct tggatttgct tacgcttatc agcttcctgg
    86401 aatgaacaaa gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat
    86461 cgttgtattg gcagatcaac gattttctag tttttcttat cgacgtttat tcccacaaca
    86521 ttggcaaaca gcaagagaag tacgaagtgt tggggaattg gaagaaggaa tacagcgttt
    86581 ttggttatca aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt
    86641 ttttgattaa cgatagttag tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa
    86701 agcaatcaca gcttgtagat catcacggtt cttacctgtc actctaatct gatcttcttg
    86761 gatttgtgct tttactttca gtttttcatt tttgatagct gtagtgattt tcttcgcgtt
    86821 ttcacgatcg attccgttga ccaaatcggc agtttgtcga gctttgccac ctagagcgtg
    86881 ttctgattcg gaaaaatgga tatttttgat tggcacattt cttttgatca gcttaccaaa
    86941 taatacatct ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa ctagattgct
    87001 gttctctaat ttgatttcta ctgtagaatc cttgaagtca aagcgattgg acaattcttt
    87061 tgttgtttgc tgaatcgcat tttttacttc ttctgtattg acttctgata caatgtcgaa
    87121 gctagcatct tttgccatag gaaccctcct gatatacata taaatattta ccatacatct
    87181 atcttatcat aggattaggg cagattctta cctgtttgct aaaattcttc tcatttgagt
    87241 tcgaagtatt ttattttaat agataaactg ttattcttac atttggaata ccaaagtagg
    87301 ggagaggtgc ttgtgagtaa aattatgact gcgtatcata aaaatgaaag attaaagaaa
    87361 atagtggtta ttttgataac cggattgacc gctgcggtcg ctttaaatta ttttttgatt
    87421 ccagccaatg tcttttctgc agggatgaat gggatcgctc aaatcatttc ctcgctttta
    87481 tatgaatggt ttcatatcaa gatagacaca ggaacattta tttttctttt gaatatccct
    87541 gtgtttgtat tagggtttct caaagtagga aaacgagcaa cgatccttag ttttgtgaat
    87601 gttgtatgtg tatcggtgat gacgatgctg cttccagtcg gacagatcac tgataatatt
    87661 ttgatgaatg ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa
    87721 atgggcttta caactggtgg gatggatatc atctctcttg tactttcaca aacaactgga
    87781 aaaacagtcg gaaactacat gctgattcta aatggtatca ttgtagtcat cgcaggcttc
    87841 ttctttaact gggaaagtgc cttgtatacg attatttcca tttatgcaat gactcaagta
    87901 attgatgcca tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca
    87961 gaagaggtaa cagaagaact agcaaaacgg atggtacgtg gaatgacatt actgccgtct
    88021 gtcggtggct attcaaaaaa acaagggaac atgatcatga tggtcatcac ccgctacgaa
    88081 atgtatgatc ttgatcagat cgttcatgaa atcgacgacg atgcatttat caatatttta
    88141 ccaactcatt cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca
    88201 actggcgtat tgccagaaat aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt
    88261 ttagaataaa tttaatctat tacatctaat aagaacattt ttataaattt aaacgagaat
    88321 cttacttcta aattctcatt tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt
    88381 gttaaggagt gtcgtacgat gagcgaagca aaccaagaac ggtctggaca agaaatagaa
    88441 ctaatcaaag aacgtattct tgctgccttg gaaatggtga ttgatcctga attaggaatc
    88501 gacatcgtca atttaggctt gatttatgat atagaattca atcctgaaaa tggagaaaca
    88561 gtcattaaga tgactctaac aactatgggc tgtccattag cagatatttt aacagaatct
    88621 atccatgacg cattaaaaga agtgccagaa gtaacgaaag cagaagtcaa actagtctgg
    88681 tatccagcat ggacgacaga taagatgagc cgttatgcac ggattgcgtt ggggatacga
    88741 tagaaatctt ggctgactag ctatgagaag caaataggaa aaacaaaaaa gccttttatc
    88801 ctttctagaa gaaaagagat aaaaggtttc tttgtttata aaattttgtc tgctatatta
    88861 taagttaatt aaataaatgt gttctattga taaaggacat tcaaatccct ttccttttgg
    88921 aatccttctt ttttacatca gtttgatttt cgtatagtat tctttctcat cttcacgata
    88981 aatcgcagcc ccattttttt gttgtgcttt aagactgcca agattttttt tatttacaga
    89041 caaaagtgcc tcatcaatgc cgattttttt tgcctcttgt agtactaatg ctaatccttg
    89101 tgtggcatat ccttttttac gataattagg ggagatgccg taaccaatat ggccagcgcc
    89161 gtttcttaat gtttcgttca gtttatggcg caaattaaaa attcctacat actcatgttc
    89221 atcactttct aaaataaaga cggttgcggg aacgtaactc tcaggaagat cgattcctaa
    89281 agacgttttt tcgcattttt ttacataatc taaaaattca gcaaatgtat agccataagc
    89341 attgttgata aatccactct catttggtcc aaagctagtt tgtagctgat aagctttctt
    89401 taaatctgct tcccatagtt tgatcagttt catcgtattc acctctttaa gtaaccttat
    89461 tcatttgttt tctactatag cactattgtt gtaaatagcc atgcctattt ttatttattg
    89521 tgtcatatcg tattaagaat catcgaacaa tacaaatatg aattacaata aaaaaattat
    89581 ttgaagttcg ttcattcgtt acttatgatc tctagaagaa agaaaacgtg aattacttca
    89641 gcgtctagaa ttccagaagg atagtaatca acagaagaaa taatcaatat ccaaatgaaa
    89701 gaagatagct gaatcgagaa aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta
    89761 ctaatcccag agtcgttcgt ttaacattga aatcgatcat atggataaaa cagatttttt
    89821 caccaaccct caatagatga tggagttgct cggatgaatt atttgtgtag atggcataag
    89881 ggagggcgta cagaaatgaa aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat
    89941 cctaaactaa aagatgtgat gcggctatgg cggaattggc agacgcgcta gcttcaggcg
    90001 ctagtgaggg tgacctcgtg gaagttcgag tcttcttagc cgcataattt gtttttttat
    90061 aaagagaagg tacacctgga gcgatgagaa agaaaataac atattatcta aaagaatata
    90121 ggaaaaattt taaacaacgg aaccagattc aaataaatca aaaaaaattt gtgtgaaggt
    90181 gggacagttt aaaacctaat agattgattc aaatagtaga tttatagatt ctgatacaag
    90241 aaatactggc aaacgatagt taaggaataa agactaaact taaactcaaa aagaggtcat
    90301 gcgaaagaat aaatcgcatg acctcttact atattttgaa tgtgtttaaa cgttcaaata
    90361 atgaatctta gtaccaaccg tttgcttccc agaatgcttt cgccgcttcc caagaaccat
    90421 aacgtgaagt aacatattgt tctgctactc tttcttggtt tgcagcagag tagtcaccat
    90481 ttaagtaaga agaatctaat tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag
    90541 aaccgcttga ttctttttgt gcgatccatt cttttgcaga gcttgtactt gctggagtag
    90601 cagcagtttc tgttacagca ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt
    90661 ccgtttgaac cgtttcttgt acaacaggtt gttcagcttg aacaggttca gcttggactg
    90721 gtgcttcttg aacggctgct tgaggttcag cagctgcttg tgcagagcct tctgttggga
    90781 ttgttaattg ttgtcctgag ta
//
LOCUS       DACEQH010000003        78404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.003, whole genome shotgun sequence.
ACCESSION   DACEQH010000003 DACEQH010000000
VERSION     DACEQH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.003"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(161..445)
                     /locus_tag="IW833_000182"
     CDS             complement(161..445)
                     /locus_tag="IW833_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306331.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(463..1353)
                     /locus_tag="IW833_000183"
     CDS             complement(463..1353)
                     /locus_tag="IW833_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ1306332.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1373..1591)
                     /locus_tag="IW833_000184"
     CDS             complement(1373..1591)
                     /locus_tag="IW833_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306333.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1561..3261)
                     /locus_tag="IW833_000185"
     CDS             complement(1561..3261)
                     /locus_tag="IW833_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ1306334.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3528..4301
                     /locus_tag="IW833_000186"
     CDS             3528..4301
                     /locus_tag="IW833_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306335.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="IW833_000187"
     CDS             complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="IW833_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ1306336.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5017..5119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5125..6096)
                     /locus_tag="IW833_000188"
     CDS             complement(5125..6096)
                     /locus_tag="IW833_000188"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1306337.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="IW833_000189"
     CDS             complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="IW833_000189"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ1306338.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8066..8884)
                     /gene="purR"
                     /locus_tag="IW833_000190"
     CDS             complement(8066..8884)
                     /gene="purR"
                     /locus_tag="IW833_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ1306339.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9051..9875)
                     /locus_tag="IW833_000191"
     CDS             complement(9051..9875)
                     /locus_tag="IW833_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1306340.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9848..10528)
                     /locus_tag="IW833_000192"
     CDS             complement(9848..10528)
                     /locus_tag="IW833_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1306341.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10551..11489)
                     /locus_tag="IW833_000193"
     CDS             complement(10551..11489)
                     /locus_tag="IW833_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ1306342.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11734..12585)
                     /locus_tag="IW833_000194"
     CDS             complement(11734..12585)
                     /locus_tag="IW833_000194"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ1306343.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="IW833_000195"
     CDS             complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="IW833_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1306344.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15453..16259
                     /gene="proC"
                     /locus_tag="IW833_000196"
     CDS             15453..16259
                     /gene="proC"
                     /locus_tag="IW833_000196"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ1306345.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16338..17894)
                     /gene="rny"
                     /locus_tag="IW833_000197"
     CDS             complement(16338..17894)
                     /gene="rny"
                     /locus_tag="IW833_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ1306346.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18305..19354)
                     /gene="recA"
                     /locus_tag="IW833_000198"
     CDS             complement(18305..19354)
                     /gene="recA"
                     /locus_tag="IW833_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ1306347.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19463..20704)
                     /locus_tag="IW833_000199"
     CDS             complement(19463..20704)
                     /locus_tag="IW833_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ1306348.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="IW833_000200"
     CDS             complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="IW833_000200"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ1306349.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21731..22615)
                     /locus_tag="IW833_000201"
     CDS             complement(21731..22615)
                     /locus_tag="IW833_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1306350.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(23393..24679)
                     /locus_tag="IW833_000202"
     CDS             complement(23393..24679)
                     /locus_tag="IW833_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1306351.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24672..25940)
                     /locus_tag="IW833_000203"
     CDS             complement(24672..25940)
                     /locus_tag="IW833_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1306352.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26192..26653
                     /gene="mscL"
                     /locus_tag="IW833_000204"
     CDS             26192..26653
                     /gene="mscL"
                     /locus_tag="IW833_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ1306353.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26712..27158)
                     /locus_tag="IW833_000205"
     CDS             complement(26712..27158)
                     /locus_tag="IW833_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1306354.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(27251..27496)
                     /locus_tag="IW833_000206"
     CDS             complement(27251..27496)
                     /locus_tag="IW833_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306355.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(27611..28189)
                     /locus_tag="IW833_000207"
     CDS             complement(27611..28189)
                     /locus_tag="IW833_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1306356.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            28403..29575
                     /locus_tag="IW833_000208"
     CDS             28403..29575
                     /locus_tag="IW833_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAQ1306357.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            complement(29625..30128)
                     /locus_tag="IW833_000209"
                     /pseudo
     CDS             complement(29625..30128)
                     /locus_tag="IW833_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
     gene            complement(30132..30992)
                     /gene="rlmB"
                     /locus_tag="IW833_000210"
     CDS             complement(30132..30992)
                     /gene="rlmB"
                     /locus_tag="IW833_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ1306358.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(30985..31380)
                     /locus_tag="IW833_000211"
     CDS             complement(30985..31380)
                     /locus_tag="IW833_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1306359.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(31377..32789)
                     /locus_tag="IW833_000212"
     CDS             complement(31377..32789)
                     /locus_tag="IW833_000212"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ1306360.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(32786..33322)
                     /gene="cysE"
                     /locus_tag="IW833_000213"
     CDS             complement(32786..33322)
                     /gene="cysE"
                     /locus_tag="IW833_000213"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ1306361.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(33644..35104)
                     /locus_tag="IW833_000214"
     CDS             complement(33644..35104)
                     /locus_tag="IW833_000214"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ1306362.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(35274..36431)
                     /locus_tag="IW833_000215"
     CDS             complement(35274..36431)
                     /locus_tag="IW833_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ1306363.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(36465..37835)
                     /gene="radA"
                     /locus_tag="IW833_000216"
     CDS             complement(36465..37835)
                     /gene="radA"
                     /locus_tag="IW833_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ1306364.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(37898..38425)
                     /locus_tag="IW833_000217"
     CDS             complement(37898..38425)
                     /locus_tag="IW833_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ1306365.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            38655..39461
                     /gene="proB"
                     /locus_tag="IW833_000218"
     CDS             38655..39461
                     /gene="proB"
                     /locus_tag="IW833_000218"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ1306366.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            39458..40705
                     /locus_tag="IW833_000219"
     CDS             39458..40705
                     /locus_tag="IW833_000219"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ1306367.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            40781..41125
                     /locus_tag="IW833_000220"
     CDS             40781..41125
                     /locus_tag="IW833_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306368.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(41242..41568)
                     /locus_tag="IW833_000221"
     CDS             complement(41242..41568)
                     /locus_tag="IW833_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306369.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(41941..43142)
                     /locus_tag="IW833_000222"
                     /pseudo
     CDS             complement(41941..43142)
                     /locus_tag="IW833_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            43259..43774
                     /locus_tag="IW833_000223"
     CDS             43259..43774
                     /locus_tag="IW833_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1306370.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(43973..44419)
                     /locus_tag="IW833_000224"
     CDS             complement(43973..44419)
                     /locus_tag="IW833_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1306371.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(44466..45944)
                     /gene="msr"
                     /locus_tag="IW833_000225"
     CDS             complement(44466..45944)
                     /gene="msr"
                     /locus_tag="IW833_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1306372.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(46298..46927)
                     /locus_tag="IW833_000226"
     CDS             complement(46298..46927)
                     /locus_tag="IW833_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1306373.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(46911..47375)
                     /locus_tag="IW833_000227"
     CDS             complement(46911..47375)
                     /locus_tag="IW833_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1306374.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(47862..49130)
                     /locus_tag="IW833_000228"
     CDS             complement(47862..49130)
                     /locus_tag="IW833_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1306375.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(49361..50098)
                     /locus_tag="IW833_000229"
     CDS             complement(49361..50098)
                     /locus_tag="IW833_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1306376.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(50165..50734)
                     /locus_tag="IW833_000230"
     CDS             complement(50165..50734)
                     /locus_tag="IW833_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1306377.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            50862..51734
                     /locus_tag="IW833_000231"
     CDS             50862..51734
                     /locus_tag="IW833_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1306378.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            52076..52948
                     /locus_tag="IW833_000232"
     CDS             52076..52948
                     /locus_tag="IW833_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1306379.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            52945..54903
                     /locus_tag="IW833_000233"
     CDS             52945..54903
                     /locus_tag="IW833_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1306380.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(55154..56005)
                     /locus_tag="IW833_000234"
     CDS             complement(55154..56005)
                     /locus_tag="IW833_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1306381.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            56128..56595
                     /locus_tag="IW833_000235"
     CDS             56128..56595
                     /locus_tag="IW833_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1306382.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(56858..57340)
                     /locus_tag="IW833_000236"
     CDS             complement(56858..57340)
                     /locus_tag="IW833_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1306383.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(57440..58303)
                     /locus_tag="IW833_000237"
     CDS             complement(57440..58303)
                     /locus_tag="IW833_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1306384.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            58901..59749
                     /locus_tag="IW833_000238"
     CDS             58901..59749
                     /locus_tag="IW833_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306385.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(59920..60261)
                     /locus_tag="IW833_000239"
     CDS             complement(59920..60261)
                     /locus_tag="IW833_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ1306386.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            60347..61054
                     /locus_tag="IW833_000240"
     CDS             60347..61054
                     /locus_tag="IW833_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ1306387.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            61041..61364
                     /locus_tag="IW833_000241"
     CDS             61041..61364
                     /locus_tag="IW833_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1306388.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(61453..62490)
                     /locus_tag="IW833_000242"
     CDS             complement(61453..62490)
                     /locus_tag="IW833_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1306389.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(62937..63920)
                     /locus_tag="IW833_000243"
     CDS             complement(62937..63920)
                     /locus_tag="IW833_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ1306390.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(64336..65106)
                     /locus_tag="IW833_000244"
     CDS             complement(64336..65106)
                     /locus_tag="IW833_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1306391.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(65099..66034)
                     /locus_tag="IW833_000245"
     CDS             complement(65099..66034)
                     /locus_tag="IW833_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1306392.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(66628..68277)
                     /locus_tag="IW833_000246"
     CDS             complement(66628..68277)
                     /locus_tag="IW833_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1306393.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(68472..69368)
                     /locus_tag="IW833_000247"
     CDS             complement(68472..69368)
                     /locus_tag="IW833_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1306394.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(69361..70041)
                     /locus_tag="IW833_000248"
     CDS             complement(69361..70041)
                     /locus_tag="IW833_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1306395.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(70042..70824)
                     /locus_tag="IW833_000249"
     CDS             complement(70042..70824)
                     /locus_tag="IW833_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1306396.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(70817..71734)
                     /locus_tag="IW833_000250"
     CDS             complement(70817..71734)
                     /locus_tag="IW833_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1306397.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(71737..72021)
                     /locus_tag="IW833_000251"
     CDS             complement(71737..72021)
                     /locus_tag="IW833_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1306398.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            72809..76036
                     /gene="fss3"
                     /locus_tag="IW833_000252"
     CDS             72809..76036
                     /gene="fss3"
                     /locus_tag="IW833_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1306399.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            76149..76463
                     /locus_tag="IW833_000253"
     CDS             76149..76463
                     /locus_tag="IW833_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1306400.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            76476..76850
                     /locus_tag="IW833_000254"
     CDS             76476..76850
                     /locus_tag="IW833_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1306401.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            76860..77195
                     /locus_tag="IW833_000255"
     CDS             76860..77195
                     /locus_tag="IW833_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ1306402.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            77278..>78404
                     /locus_tag="IW833_000256"
     CDS             77278..>78404
                     /locus_tag="IW833_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1306403.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRD"
BASE COUNT        22901 a        16473 c        14131 g        24899 t
ORIGIN      
        1 taataccgag aaaatggttt ttgaacatca cttattcaaa ataaaaaaag attgtgacac
       61 gaattttgtc acaaccttta aatgtattcc tagagcaaag aagctaagcg aagaaatcca
      121 attctgatta tttattgtat ttctttgttc agcatccttg ctaaaagccc atattggaca
      181 aagtattcac tacacccatg atctcatctt ttcgagaacg tgctttttct gtcggatctt
      241 ctacctcttc tgtgtcaatt cgaacaataa atttccgatt catcgtcacg taagcaccgt
      301 cgttgatcaa aataaacagt tcgttctcat tatagtctgc acaggcatct ttccatacgt
      361 caaagccgtt tttttcagaa gggatatccc gtttaaccac agtttttcca cttaattcgt
      421 ggaacgttac gtcataatat ttcattatct ctacctcttt cgtcattcaa tattttgtac
      481 ttgttctctg attttttcta agatcgtctt agactggacg acttgatttt taatttcgat
      541 tgcatttgat ttggaaccga tcgtgttgat ttcccgattc atttcttgga tcaggaaatc
      601 taattcccga cctacaggac attgtgcttc gataagttca tctagttttt gtacatggat
      661 cgccagacga tccagttcct cgtgaatatc tccgcgttcc aacaagatgg ctagctccgt
      721 caacaaccgt tgctcatcga ctttcgctcc aagaaattcc tccagctttt tttgatacct
      781 tatttgaaat tccttttcat agatttctac aaaactatcg agttttttta gtacttcttt
      841 gaattcttca gtattctttt tcaacactaa agctaaagct tgaccttctt tttctcgact
      901 cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg ccaattcttc
      961 catgctctct tccggttttt cttctaccgt tacgaagttt tcgtccagcg cgaagcgttc
     1021 cgccatccga ctaattgaca cttgattttg actaccgtat cgttgttgaa ttccgtctgt
     1081 caattgagtg ataagtgaat caatcaattc ccagttgatg acgatctgtt tttcgttttt
     1141 gccaatataa gctagattca caaatacttc gattcgaccg cggcttaact gctgtttaat
     1201 aagctgacga aaatcgtttt caacaaagtt caggatttta ggacttcgta tttgaacatc
     1261 tagaaaacgc tggttgacac tttttatttc aatatctaac tgatacgctt ttgtttcgcg
     1321 gatggctttg ccaaaaccag tcatactttt cattcgacag ggtccttcct cattacatat
     1381 tttctattgc tttttgcagt tgttcaaact cttcgttcgt caatgtcagg cctttcccca
     1441 ttttttcatg gtctggcgcc caatctcgaa gatcgaattt tggcgggcga ccattccagc
     1501 tgactaggtt cagttcttta cgccacccct tgttgttttc tgacaatata gcgatttctt
     1561 ctacgatttc atatgaaaat tcttgtgcca tggttgttcc tccttgagat aatataaata
     1621 taggatcagt aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac gatccttgcg
     1681 ttttccttgt agcagataat caaaataaac agttttcgtg cgctcatttt gtattttttg
     1741 ctggtatttt cgataccttc gccattcttc agtacttaat tgaccaattt gtgccaataa
     1801 atcatccggc atacttttta tatcttgttg gcagatcatt tgaatcatca tgtctacttg
     1861 atagatttca ttgggcagta aaaaatgttt ttttgctaaa agttctagca tcgccaatcc
     1921 gcatgtctct ggtacgaccc tgttcttata taaattacgc agtttttgcc aaaattttat
     1981 gcttttgaaa aaagataatt ctaactgaat ttcttttgtt aagtaagaca aaaatcctcg
     2041 ccagtatgtt cttgcaggtt taacagaatg aaatgtatac cagttcccat agtaatagcc
     2101 taatcgtttt ttcatttcca gatagcctaa ttgtaaattt aattggttcc tttggctatc
     2161 aaaaagcaga aagctgccta gtgtccacaa tgtttgacat ttccaatgga taaaaatgtc
     2221 tggcatacga atttttttcg ctgggcctgg cccttgcaca tctacgacaa atgcctctgt
     2281 tgctcctttg ttaatcgcaa tatcaatagg tattttgttg cgatacccac cgtctgcata
     2341 tttatttttt ccgatctttc gataagccat agcgggataa aatgaagcag aggcgagtat
     2401 ccaatctggt atttcttctg tttttgtctg ttggatatca gtcactactt ccgctagttc
     2461 tggcagttta gtagacaccg tatataaacg tattttggga ttatgtttga gtaaggacaa
     2521 atccaaattg tctttgacca tcttttccaa tggagcagaa gaaaccccgc cttcaattag
     2581 tgctgttttc gtcatttggc gtctttctcg atagaagcgt tcacgaaggt cttcaactgc
     2641 agccatttct ggtaacgcaa gcacttgtct tgtcgacagc tttttccata aattcacagc
     2701 ttttttcata tctccttgta aaatcaatgc gccgttcaac gcacctaccg aggtacctgt
     2761 gataatctca aagttgatat catgttcctt cagcgcctgc cacacaccaa tctgataagc
     2821 accgtgcgct cctcctccac ctaaaacaag tgctgtatga tagaccagct ttttatttaa
     2881 ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc aaccattctt ctgatagagg
     2941 agattgaaat aaaaaacaac agttttcttt aataaaaaaa cgagcgcaag cttccatata
     3001 ccgaaaaaat tttttccatt caaaagtttc accttcaatc aacagattat taaaaataac
     3061 atctcctcta cttttagcaa tagtaaaata gacggtatat tttttcgata ctgctttaaa
     3121 agcacattgc ttttttgcag aacgccaaaa agcgtctcta ctttcttgat gactttgaaa
     3181 aaatggaagg ggttgttttg cttgctggat aggcgaatac agcgacattt tctgtgaaaa
     3241 gtaaactttt tttatctcca tcgcgctctc ctttttcttc aagtatacca gtacgtcata
     3301 aaaaggtcat gagtctcgta ttcgactttg tagattccat aagaaaacaa ttcataaaac
     3361 ctcctttaaa taaaaaaata caaaaatcac aaaaattaat tgaaaatgag agtgttttca
     3421 atgaaaacgt gattttgtaa tggaatttta aaataaaaga aacaagcttt tttaatgcaa
     3481 atgttgtaaa ataaaagaga ttaagaggaa ataagaggag gaacatgatg actgagtcag
     3541 tttatgtaca tattgatacc acaagtaatg ctgttttaac aaaagggctg actgctactg
     3601 actttgcaaa cagtatcgtg cattttcctc aaaacttgct cttgcttgat ccatctgctt
     3661 ctgctggaga atatgaatcc catacagggc tgaaagtaat ccgtggtaca gaaaatattc
     3721 agcgtttctt ttcctcttct cgtaacaggc gtggattcga tgaattgaag tggatcgact
     3781 ttactgattt aacgatgctg aaagaattga cacctttaga aatttcagaa ttattatatt
     3841 ttggtcatat gaaaacacat cttcattcgc cgttttttta taaactacag aataattttg
     3901 tatacttcga tcttaatgat cagctgaatc ggatttatta tcgctattta gatgaatttt
     3961 ataggatttt tgctagtaaa ttaagttcga tcttgtcaga taagatcaat gtaaaaaaat
     4021 cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt ggaattattg aaagaaatgc
     4081 gtaatttgat gcaggaaggg atcatttttt cttttgatca agttggtttg atcgatggta
     4141 aatatcgtat cccaatctat gtgattgaag attatatttg gaaattaaaa ggcacccggt
     4201 atcgagaaga ggaaatcatc gctactttaa tctactctgt caccgatcga cactggcaat
     4261 tggtagaaga tgccgatctt tcttatctgc cattgaagta gatctatttc ttatcaaaaa
     4321 ataaaaagga aacaagaaac taggacatga ttctgtctca agtttcttgc ttctttttta
     4381 tttacagctc ctgtcgttta tctggcacaa aaatcgaagg agtcgtttgt tggatcccta
     4441 ataaaacgat gatcgttaca atagctgtac tcaacccgct ggcaccattc attacgaaag
     4501 aaaagagcca aggattcatc ccccatagtg catacgctcc ccagaaaacc actcctgcaa
     4561 taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc accaaacacg atgctttttc
     4621 taatttgttt cgtattatcg tctacatagc ctttttggac atttgaagcc atgagccctg
     4681 ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa cacttgaacg acacttaaat
     4741 aataaacttg tcccaacgga aaatgtagga tgccccaaat gaaacctgcc agcatccctg
     4801 cctttgtccc tcttcttaac gcaaaaagag tcaatggaat ctgccctaat gaaatagaaa
     4861 aactgctgcc gatctggatc ggaatcaacg acaacaccat cgctaacgct gcaacaatcg
     4921 ttccttctac ccaaactcta ttttttccca taaaaaaact tcctcctttg aataaaaaag
     4981 tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc tgtttattca caattcctac
     5041 gctcgcataa ccgatacagg tacgaagggt ttagaaatag attcaatctc agcccaattg
     5101 gactcccctt tgtgagttta tttattattt attttttgtt tcaaacaatg gactaactgg
     5161 tttgttttca tggatacgtt tgattgcatc tcccattagt ccgccaacac ttacttcatc
     5221 gattttgtcg attttacgat cttctggaag atagatcgag tctgtaacaa ctagacgctc
     5281 gatagctgaa tcttctattc tttgtaaagc agggccggat aatactggat gtgtacatga
     5341 agcatacaca ctagttgcac cagcttcttt caaagcattg gcagccaatg tgatcgtacc
     5401 tgctgtatcg atcatatcat cgatcagtac acaagttttg cctttgactt gtccaatgat
     5461 attcattact tctgcaacat ttgcttttgg acggcgtttg tctatgatcg caatcggaga
     5521 tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca ccatggtctg gtgaaacaac
     5581 gacaacatca tcgcctttga ttcctttttc taagaaataa ttggcaataa gtggcgcacc
     5641 catcaaatga tcgactggaa tatcaaagaa accttggatt tggactgcat gaaggtccaa
     5701 agtcagcatt cttgttgcac cagctttttg aatcatgttt gctactaatt tcgctgtgat
     5761 tggttcccgt gcacgtgctt tacggtcttg acgcgcatag ccataataag gcatgactac
     5821 gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc atgatcagca actccatcaa
     5881 gttatcattg actgggctgc tcgttgattg aataacatag acatgcgatc cacgaatact
     5941 ttcttcaatg tttacttgaa tttctccatc actaaattga gtaacagatg attttcctaa
     6001 ctcgacgccg actgctgctg caattttctc tgctaatggg cgattggaat ttaacgcaaa
     6061 aatttttaat cttgggtcaa agtaatgttt tgacatgggg acctccactt tctttttcac
     6121 aactctagaa ttctacctaa taaatagtag tcatattttt taaataaatc aagtgatttt
     6181 gcaaaagtct tagtttagat aagggagttt ttttgcatat ccttctttat tgacttgtct
     6241 tgctctcgca attgccagcg catattctgg aatattgtct gtaattgttg acccggcagc
     6301 aatcgaactg tttttagcta cctcgacagg tccgatgata ttcgtgctag aaccaataaa
     6361 gctgtggtcg ccgacagtcg tatgatgttt gttctttcca tcatagttga cgaagacaac
     6421 accacatccg acattgattt cttctcccag cgtagcatca ccaacataag tcaagtgacc
     6481 gactttagtt cctttgccga tttgagcatt tttcacttcc acaaagtttc cgatatggac
     6541 gttttcgcca atttcagctt ttggtcgtaa atgtgcataa ggtcccacat ctgcatgttt
     6601 cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg acgtgatcct caatcctact
     6661 gtctacgatt tttgaatgtg acccgatgac acaatcagaa ccaatgactg tatttccttg
     6721 gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt tctacgcctg catcgatgta
     6781 tgtcgttgct gaatcgacaa agcttacgcc attgaccata tgcatctggt taattcgctt
     6841 tctcatgatc tcatttgctt tagctaacgc gatccgatca ttgactccca ttgattcttc
     6901 aaaatcatcc gtttgataag cagcaactgt atggccttct tcttttaaga tttcgatgat
     6961 atctgttaga tagtattcac cttgagcatt gtttgttcca actttgctta acgcatcgaa
     7021 caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt tcttgtacaa gagcttcttc
     7081 tggtgttgca tctttttgtt ccacgatttt ttctacgatc ccaatatggt cacggatgat
     7141 tcgtccatat cctgttgggt tttctgcttg tgccgtcaag atcgttgcac ttgcattttt
     7201 tccttggtga tattcaaaaa gattgttcag cgtttcagta gtcaataatg gtgtatcgcc
     7261 actgataact aacgttgttc ctttttttcc ttccaagaaa gaagcagctt gcaaaacagc
     7321 atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta ctgcgttctc ctagttgtgc
     7381 tttaacttgt tctgctccgt gtccaacgat cgtaatcact tcatctggtt tcgtttcact
     7441 gacccgatta atgatatgtt ctaccatagg tttaccagaa acgggatgta aaactttata
     7501 taattttgac ttcatgcggg tacctttccc tgccgccaaa ataattgcat aacgtgcgtc
     7561 caactgcgtc actcctaaat atagtttgct gatttcagtt tatcccagta tacccatttt
     7621 ttcaacaaaa caagtgattt tcttttatcc cagcgttttc tggataaaaa aatagataga
     7681 ccataacggg cctatctatt tggtaaccgg tgctctcctg ccaactcctc ggtcacaagt
     7741 cacactactc ataaaacatg aaaagctgtt ttttgaaatc gtttcttgtg acttagagtt
     7801 aaacggtagg cttacaacct cttcattaaa cgtttttcaa tcagctgacc ttcggtatta
     7861 gctcaataaa cggcaaaata tgaggagcaa tttttccgtt tattgagcta atactcagga
     7921 caggacgctg atttcacaac ctctagataa acggtgttca aaagccagtt tctcggtatt
     7981 aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca aaatttttct tactactcga
     8041 aactaagcag ctttttcaca acctcttatt ctgaaaaata atttcctggc acaaccgtaa
     8101 tatttttcgt ttgagtatct acatctttga cataaagcag tgacgtataa tcatcgatgg
     8161 cgcgatgacc gttgaatttt gattcggcaa atacagtgat gccaacaagt tctgattcga
     8221 attcttcaat caagctcttc attccgttga ctgttccgcc gcctttcatg aaatcatcca
     8281 ctacaaggac acgtgaacca cgtttcaaac ttctcttaga cagttccatc ttttcaattc
     8341 tttcagaaga tccagacaca tagttgacac ttactgttga tccttctgtt atttttgagt
     8401 cccggcgtac aataacgaat ggcgcattca gataatagga cacagcttgg gcaataggta
     8461 cgccttttgt ggctacagtc attacagcat cgattttttc accaaggtat ttagaagcaa
     8521 tgattcttcc aacttgacgc agcagttcag gttctcctaa caaatcagag agataaacat
     8581 atcctcctgg aagcagacgg tcttgttctg atagacgttc tgctagagat tcaatatatt
     8641 ttttcgcctc ttcatatgaa atttcaggga tgaacaatac accacctgca gcacccggga
     8701 ttgtttccaa ggttccatac cctcgttctt taaatgtttt cttgatgatt cccaaatctt
     8761 cactgataga agatttagca gattcataac gttcggcaaa gcttgttaag gaaatcaatt
     8821 catgcgggtg attcaataaa tactgtgtca tgtcaactag acgttcactg cgacgtactt
     8881 tcacctattc caactccttt taagaaattt tatcgtttta cattatataa gtttttcttc
     8941 aaaaattcga gcattttatg aaaaaataag aaaatagttc ggtatttatt aaaataaaaa
     9001 gaagcctata aaaaattttt cgtttttata aggcttcttt tctttataat ttagttattt
     9061 tttcttctga aggcaaccaa tattcgtttg atgatattta caatcaggaa caaagcaata
     9121 aagactaatg tgattgttgc tcctggaggt gtatctagat aaaacgagct agttagtcct
     9181 gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt gaagcttttg
     9241 cctaatcgca taccgattgc tgcgggaaga accatgattg cggatatcaa aagtgcaccg
     9301 gcaatcggga tcatcacagc aatagccaca ccagtaatca cattgaacat catcgacatc
     9361 aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat caaaagtcag aacatacatc
     9421 ggccgtttga ataaaaagaa caatataccg atggcaacga ataagattcc taaaaagatg
     9481 acttgctccc atgtaatggt cacgatcgaa ccaaacagat aggattggat acttgttgct
     9541 gaattccctc cgctcaaatt catcaatact aacgctagcg ccaaaccacc agccattaaa
     9601 atggcaatgg agatttctga ataggttcgg tagatcgttc gcagatactc caatatcaca
     9661 gctgctaata caacgactag catggtcgta agtgtaggat taaggttcaa aaagaatcct
     9721 aacgctaccc ctgctaaaga gacatgagaa agcgtatcag ccatcagcga ttgtcttctg
     9781 atcactaaaa acacacctag catcggggcg attccggcaa tgaagatagc ggcaagaaac
     9841 gctcttctca tgaattcata tgaaagcaac gccattgaga atcctccttt ctcaccagtc
     9901 ggatctgtct gtccgcatac tgtttgatat cttcatgatc atgggtgatc attagaactg
     9961 atttcccatg gacatgcgca ctatgtcgga gaagacgata aaattcattt ctggattctt
    10021 catccatacc tgttgttggt tcatcaagaa caaataagtc agggtcagta gcaaaaatac
    10081 gcgctaaact gattcgctgc ttttgtccgc cagacaattc acctattcgc cgatttcgca
    10141 tatcccacat ccctacagct tctaaagcac gctgtacatg ttgatgatct ttttcagaga
    10201 gacgcttgaa ccagcggcct cttggatatc ttcctgaacg gactaattcg atcaccgtac
    10261 taggaaaacc tgcattgaaa gatgcaactt gttgaggaat ataaccgata cttaattttt
    10321 ttccttcttg atttttctca gcaatcttta tcattccttt tgaaggtttc agtaatccta
    10381 acgatgcttt caccaaggtt gacttggctg ctccattttc tccagttaaa atgacaaatt
    10441 cacccgcatc tacatgataa gagacatctt ctagtacagg ttcatcatca tagtaaaagg
    10501 tcaaatcaga tatttcgata taacgcacct tacaacctcc attctaaaac ttaattgata
    10561 ctttctttta aagctttcag atttttttcc atgacagaaa tataattttc gcctgctttg
    10621 atttgttcat tagtcaagct ttctaaagga ttcaacactt ctagcttcac tcctgtttca
    10681 ttcgacaatg tctcagctac cttcgaagag gcattttctt caaaataaat cactttgatg
    10741 ttgtttttct tcacaaattc ttttagctca gctaagcgac ttggtgtggg ttcctgatct
    10801 ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa
    10861 gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc ggagtatttt
    10921 tgatccagtg cttcaagctt tttgatataa gcatcggcat tcttcgtaaa agtttccttt
    10981 ttatctggat atttttcgcc taattcttca gcaatcgttc tgacttcttt tatagctaat
    11041 accggatcga gccaaacatg tggatcgtat tcatgtgaat gaccatgccc ttcttctgat
    11101 tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga ttcctttagc tgcttcgatc
    11161 atcaaggtct gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct
    11221 gaactattat acacgaaaac atctgcatcg gaaatttctg ctctttcttt tgcacttggt
    11281 tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct
    11341 acgatctctt ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt caaatcctct
    11401 tttttgtctg cttccttgtt cgtatttcca catgctgcaa acagtaaagc acctatcatt
    11461 accgtagctg tcaaaagata ttttttcatt tatatgctcc tttcaatgaa caaatcggaa
    11521 ttttacgatt tattaacgtg aactaatgta tcaaaataaa aaaacacagt caatagaaat
    11581 ccatcaattt ctgatttaag catgtatatt cttgattaaa tcgtaaaatg gatgagttgc
    11641 tgctcatatc tagccatttc actacaataa aaaactgaag aataccattt taggattcct
    11701 cagtttttca tcacctattt tacttcttat attttattgc agggttcgta ctaaataaac
    11761 ttcctcacaa aaacctttta acccattata tactctctgc gctcttgaat aatgttgaca
    11821 caaggcaaaa acggttggtc cgcttccact catcaatgca gcatctgctc cgtacttgat
    11881 catacgctct ttgatttgat ggatgactgg atgccgcttt cccgttatat cttctaaact
    11941 gtttcccatt tttttgatca tctgctgata atcttgctct cggatggctt cagcaagtcc
    12001 gtggatgtct tgatgttgga tggcttgcgt atctacttct ctaaacactg tacgtgtaga
    12061 gacactcatt cttggtttga ccaacacaac ccagcactgt ggcatagaag gtaatacttg
    12121 gatttcttcc cctttgccac cgacaaatgc cgtacttccg tagacacagt aaggaacatc
    12181 tgtaccgact tccaaaccga tttctgccag ttcttctaac gaacagccca gattccataa
    12241 ttggttgatc cctcttagag cagctgcaca gtctgtactt cctccgccta atccagctgc
    12301 tactggaata ttcttcttaa caaagatctt gacaccggtt ttgatattgt atttattttt
    12361 tagcaataaa gcagcttgat aaacattgtt ccgttcatct accggtaaaa atgctttatt
    12421 tgtttctaca atgattttgt cttctgttat ttcttcaaaa aatagatgat ctgccaagtc
    12481 tacgctcgac atcaccattt ccaactcatg atacccatct tgccgtctat ataatacatc
    12541 cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat tccatcctct cacccattcg
    12601 aaaagtctaa gtcgcaagta ttctatcata gggaaaaata aaagtatagc ttatgtctgc
    12661 ctagtggttg attttgtcat cgtttcaaca gctttcacag aattttctct tcataatcca
    12721 aacaagttct agtgattctt ataattctgt gagcacttct tctgccaaac tcggaacatt
    12781 tcctttttct tttcctatca gactgagtag ctgcatggta gcaaatccga aattacttcc
    12841 tttcctataa gcaaaataac gtccttccca atgatcgaca tattttaatt tcttttcata
    12901 agcagactgg aaggaataga aaaaattacc ataattatat acgatatgca tgactttttc
    12961 ttgaaagaaa gaaaaatctg tttctccaac attagctaat ggagctaagc ctagattggc
    13021 ttccgagtag cccattttct cgtatacaat aaacagattg gctaacagat agtcagctaa
    13081 ttcctcagat tctttaggta agacccgcaa caaatcatat cccgcctgct tcttcgtcat
    13141 cggctgagcg gtgatgaagc caacgacttt ttgatcttta cggacaatac cgacaccgct
    13201 agataataca tatgatttat caaatcgtcc cacagagaag tatttttctc gtgcaccttg
    13261 aagccatttt tgtgaaactt catttacagc ttgatgtaat tcttccggca gttgatcgta
    13321 ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat tcaagttgct gactcagccg
    13381 agacaagtct tctgttgcta gatcaacaat ccctgcttca cctactttcg tgaagtgaaa
    13441 acccaattca tgaagcatca ctacgtagct ttctgatatc ttataaaacg caagctgata
    13501 gcctaaacaa tctgcttgtt tcataaactg attcgttgct gcccgcatca ttttaggatt
    13561 gccaactgga tctcctaaaa caaagcattt gtttcctttg atctgataac caaaaacgac
    13621 ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc gaagctgatg
    13681 gcttgtagct gtgcctccgt atgtttccaa taaacggtca aaacggtctt tttcgtactc
    13741 actgccaagc ttcgtagtat cttcagctaa atattgataa agcgccacta aagctagcaa
    13801 tgagatgccc aaaccaataa gccctgagaa ccacacatca tctgaaggaa acaaaataaa
    13861 atgatttcct gttattcgac cgactaaaaa gcgtccgctg tgatagcctg caattgcgta
    13921 aacgataaac aaaaagccga acaagctagc atcaaagatt atagctcccc aagaataaac
    13981 gaattttgtt cgataaaatt cttttctgga taaccacaca gctaacagaa tcaagacata
    14041 taccaatatc aactgccaag ataacgtacg tgtgattgta tttaaaatac caaaagatag
    14101 taaagcaatc gtaggcagaa atgccttttt gacttttact gaaagtgctc tagcaaggcc
    14161 gagtaataaa aagccaacca atagattcac tgtctgatct aaaaaattga atgagaacgg
    14221 caataatact tgaaatagcc gactgattgc agacagatta gtgattgttg atagaagtac
    14281 catcatgatc cccgcaaaat agacagcact tacgagcacg atatgcgcaa ccttttggaa
    14341 aaacagtcgc ggaatgttat ctaaaaatcg attgattttg actcctgtat gtgtgatgaa
    14401 caatagaagt cccgtcataa aaggtagaac ataataaaac aaccgataat ataatagcca
    14461 aactactgct acatcttgcc gaacaggcaa ttgactcatt cctaagatca tcaaaacgtc
    14521 aaaagtcccc attccacctg ggaccatcgt cagcattcct atcaatgtag caatcatgaa
    14581 cataggatag atcgaaagca tcgaaatatc tattctcatc aacttgccga tgatcaaaaa
    14641 taccgcaagg gctcccgtcc attgtccaaa ggatgccagt agcagaccaa gcaagccttt
    14701 tggataaaac tcgaagaaca atgtctttct ctttagataa gcaaaaaaga aaatgactgg
    14761 tgctaaaaaa ctaccgccga aaagccagat ccaatagctt cgaaaaatac tttctgactg
    14821 aataaaaaag acatcaataa atgtaagaaa cgaccataca gacaaaccag tcagcataaa
    14881 tagtgcgact tttgatacag tggctaaaac catttttcta tttgtctgct ttccataaaa
    14941 actagcccgc agacttgctc ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt
    15001 tgttacccac gcagcaagaa gaaactcttt tctttccatt tttggttttc cttgtttttc
    15061 taagactctg atcgtcaccc aatcatacaa caacatcgga gtgacaccca gcagtccagc
    15121 cagaatcatg agcaggatcg tcatgcggct ttgttgtccc atcctttgcc aaatatcttg
    15181 ccaactcatg ccattagcaa tatttgcgac ttgatttgcc acaaagacta acacggagcc
    15241 gaaaaaaatc agttttaaaa tcagactatg ttcctttatc cattgcagca tcaagtatcg
    15301 tttatttttc acattttccc attcctaact tgtatacttt tgtgtcatac cccaatgtta
    15361 tcttatttat tttaattttt ccactttaga cctgttcttt gcttgcgatg atagaaaaag
    15421 aaagatatcc taatgctaaa ggagaggata acatgaaagt aggatttatc ggcacaggga
    15481 atatggcaca agctattatt gatggtttat taaaaaaagg aacagtctcg aaagaagagg
    15541 tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc
    15601 atgcatgcaa aagtaatcag gaagttgcat cacaatcaga tatcgttctt ttagccgtga
    15661 aaccaaaaat cattgaaaaa gtattggaag aactaagaga atatagccag aaagtcttat
    15721 tcatttctat tgcttcagga aaatcattaa cagatttaga aacagcgctt gaagatcctc
    15781 aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg
    15841 cgatttgcaa aaatgaagct gccacggagg aacagcttca gcaagctacg atgatttttg
    15901 aagcaatcgg cagcgtctat cctttagcag aatcagattt cagtacattc attggccttg
    15961 ctggcagctc acccgcattc gtttacatgc tgatcgatgc gatgggacgg actggtgtac
    16021 tgaatggttt gtccaaacag acagccactg agattgctgc aaaagcaatt atgggttctt
    16081 gtcaaaaatt cctttcaagt gataaaaatc catgggaact tgtagaccaa gtttcttctc
    16141 ctggcggtac aactgttgca ggtgtagttg ccttagaaga agcaggattc atcccagcaa
    16201 tcatcaaagg aatcaatacg acgatcgaga aagatcttga gatgttgaat aaaaaataat
    16261 gatagcaatg ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt gccgcggctc
    16321 ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt
    16381 actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact
    16441 aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt
    16501 ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga
    16561 cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct
    16621 gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca
    16681 tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc
    16741 gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg
    16801 agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca
    16861 gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat
    16921 cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac
    16981 gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc
    17041 atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc
    17101 cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca
    17161 tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt
    17221 ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct
    17281 gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga
    17341 tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc
    17401 agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca
    17461 attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct
    17521 tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt
    17581 ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct
    17641 tgagatttta attctaactt actttctctc agctcacttt caacttcaga acggtatttc
    17701 tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt
    17761 gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt
    17821 gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga
    17881 atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc atctatacag
    17941 cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa
    18001 aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag
    18061 aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat
    18121 ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta gatgaacaga
    18181 tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct ttattcaata
    18241 aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt gtttatcata
    18301 atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct gggacagcga
    18361 cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc atcatttcag
    18421 gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga tcttctttat
    18481 aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg tctagaagtt
    18541 ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt ttgaaaggag
    18601 gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata tctgtacctt
    18661 gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc
    18721 gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca cggatttggt
    18781 tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta cgcaatgctt
    18841 gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg tcgatttctg
    18901 ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc ccacttgata
    18961 ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt
    19021 catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc tcagcatcaa
    19081 tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt gcaactgttg
    19141 ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg taaccgccga
    19201 cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt tgttggtcga
    19261 ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt tttttcagtg
    19321 cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt atatttacaa
    19381 atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa
    19441 accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa agatcaaatc
    19501 cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga tatttttccg
    19561 aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat tgaccttcta
    19621 acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt gctatctcac
    19681 gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat tctgctaata
    19741 gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta acgaatccgc
    19801 ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg
    19861 tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc actacgactt
    19921 ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt tcttggattt
    19981 tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt ttcactgtca
    20041 gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg atctgtgtct
    20101 caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa
    20161 ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt
    20221 ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac gcatgtgtat
    20281 ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga accccgcctt
    20341 cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt gtttcaaaat
    20401 atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg aggtgagccg
    20461 cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat aagagtatga
    20521 gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga ttatctccta
    20581 caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac aagaaagtgg
    20641 cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt atttctgctt
    20701 tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt ttatttaaaa
    20761 agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt
    20821 tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa agctgttttt
    20881 tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc cactaaacga
    20941 tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca cacctgaata
    21001 gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac cgatcaggct
    21061 gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag ccgtcttgac
    21121 ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta aacgcaagcc
    21181 tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg cctttccttg
    21241 gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg ggtccgcaaa
    21301 cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta accagtctgt
    21361 gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca gtgtggtgtc
    21421 tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga aaatcgggat
    21481 catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga ctcctttctt
    21541 ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt tccaaatgaa
    21601 tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa gaaggaccgc
    21661 gacacagatt tgtcacgatc cttctttttt atacctacaa gtaaacagat gcgtaaacta
    21721 atttatacga tcattctgcg tattgaagag taaggttcac attttttgga ctctgattgt
    21781 tttcccctgg attaactggt acggattctc cattcgcttt gatcgttgca ttgcttgctg
    21841 caccaatcgt gatggtcgca tttgttgctg tttccggtaa cacggtactc tgtgtttcat
    21901 ttgctgccag tgtcccttga taaacatagg cattgtctac tagcacacct aaccatacac
    21961 gattgacagc agtaaattca agggttacag gcttggtagc gtctgtgata tttactgcga
    22021 tggcactgcc agaatcttct cctgtagcta ttgccatttt cttttcttca cttgattttg
    22081 ttgttgattc tactgtagaa gactctttgg tctgcgtgct ttgctctgag gaagaggact
    22141 cctcgactga tccttctact ttaagcgaag aagctgtgtc tccgattatt ggatcagctt
    22201 gacgatcttg ccaagtcata tagcccacga caacgacaat cgccaaagca accaatccta
    22261 ataaaatgac aggaagactt gtccaaaagc gacttggatg cgattcttcc tgatgcattg
    22321 cttttctaga agtacttact gtttcaggct gcggacgttc agggaccgat cctgtcaatt
    22381 tgcttttccc atcaaaaaca tcgactaaat ggtctccatc ttctcctaca gcttcagcgt
    22441 attgccgaat aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt
    22501 cgattgcttc caaataacgt ttttgaattt ttgtaatctg ctgtaattca tctaaagata
    22561 tttttttatt caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt
    22621 catcttctcc agtctgtttg attctccgaa gcatctgctg ttgcaacgaa agacgttatg
    22681 tttgggtgct gtgacaaaag tcatgtcaca gctccttttt tccgaataaa cattgttctc
    22741 ctgccaactc ctcggtcaca agtcacaata tccaataaat ataagaagct gttttttgaa
    22801 cttgttcctt gtgactcgga gttaaacggc aggctcacga cctctttctt aaacggtgtt
    22861 caatcagctg ttcctcggta ttagctcaaa aaccggcaaa atataaggag atatttttcc
    22921 gtatgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttcc ttaaacggtg
    22981 ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacatta agaagctgtt
    23041 tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct cttggtacac
    23101 ggtgttccag aagtagcttc ttcggcaata agccgaaata tcacaaaaat ttgagaagca
    23161 attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa cctctttgat
    23221 tacttcgttt gattttattt taatataagt tgattaaaag agagcatgat ttgttttatt
    23281 ttctcactaa tttttccaac acaactaaga ataccataaa agctgatttt ggaggaggtt
    23341 cctactacaa gtctaaaacg aaatttaaga cttttcctca taaaatactg gtttattttg
    23401 gtctcatgta aaactcgctg aaggcctctt cacttataaa tgcttcccca gctgccagta
    23461 cgtctttcat ttggatcgaa tcgatgattt caggaagatc aaataaagtt cgatcgccaa
    23521 ataaactttg cgtaaattga ttggcaatat attcgatcga atttaatgat tggaaatatt
    23581 gtccaagcat tttcttcttc agcaaatcga gatttgtttc tgacacttcc ggatcatcag
    23641 cgaatccaag aatgatttct ttgacttttt ctgctgcttt ctctggttcg tccgtatctc
    23701 cgctgaagtc cgcaaagtga aattcacgat ccaagctgaa ttcaaaacca aagctgtcat
    23761 cgataattcc ttgattgtac atcgctagat agtttcgaga agtattgcct aaaatcattt
    23821 ggaacaacag attgattgct gttttatagc gtaacagttc tgttccttct tgcggcaatg
    23881 tgtctaatcc tttaatacca agtacaaatt tatctcgtgt aatggctgct tccaatgcac
    23941 tttgtttgat gatttctttt gtgttttcag ggaaataacg tacaatttct tgctttggag
    24001 ggaaattttt tgcttcttgg ttttcccgaa taagtttcat caatttctca ggctccatct
    24061 taccaactac aaataaaacc atattgcttg gctgataaaa cgtccgatag cacgtataca
    24121 agtcttgagc tgtgattttg tcgatacttt caaccgtgcc cgcaatatcg atatgcaatg
    24181 ggtgtgtagg atacaagtta tttagtatgc caaaaaacat ccgccagtta gggtcgtctt
    24241 catacatttg gatttcctga ccgatgattc ctttttcctt gtttacagtt tcttctgtga
    24301 agtaaggtgc ttgtacaaaa tcgatcagag tcgtcagatt cttttctacc tgatccgtcg
    24361 tagaaaacag atagctggtt ttcgtaaaac ttgtgaaagc attggcggaa gctccttgtt
    24421 tcccaaataa ttgaaagaca tcaccatctt ctttttcaaa caacttatgt tctaaaaaat
    24481 gggcaattcc atctggaact ttcacctttt ctttttcacc atagggaata aattcgttat
    24541 cgatcgaacc ataattcgta ctgaacaaac catatgtctt gtgatagtca tttttaggca
    24601 acaaatacac ggtcaaaccg tttggcagta cttcatgata aagtgtttca ttgatttgct
    24661 catattctgt tttattcatt tgcatttcct cctgctaaaa agaagattgc ctgcaattcg
    24721 atttgctcag ctacttgttg tatctctttg atcgttacgc tctccatacg tttcaaccat
    24781 tcttcatcag ataattttgt ttccggcagc cagctgtcca aaaaagaagc ttcgattgcc
    24841 gcttgcggag agtctagaga aagcagatat tggtttctca gcattgcttt tgtctgagac
    24901 agttccaact ctgttatttc accatttcgc aaactttcta attgctcgtg aatcagatga
    24961 agtacttgat ttcggttctt ttcatcgata ccggtttgga cactcatgaa tcctcggaac
    25021 gtatccacgc tactagaggc ataataagct agactttctt tctctctgac attcatgaaa
    25081 agtttagagt gaggaaaacc gccaaataaa ccgttaaaga ccattagcgc aaaacgttct
    25141 ggttcatcgt aatacacatt tgtctgataa gctaggttca gctttgcttg aacgattggc
    25201 tcacgtactt gtcgttcctt cacgatattc acttgaggtt gggtataaaa cagatctgga
    25261 taaactgttt cccgcgactc aaatggtaat tgcccaactg cttcagctgc tttgtccgga
    25321 tcaacatcgc cgacaacaaa aatgtcgatt tgatcatttg ccatcatttc ttgatagtag
    25381 gacgctagcg aactagcagt gatcttgtcc aaatcttcga ctacgcccaa ccctgggatc
    25441 ttttgttccg ggctgttttc aaaataaagt tcttgcaagg cataggaagc aaaagtttgt
    25501 ttatcttcct tcaaactttc caaatataga cgcagattgt tcttttccaa gtcaaacgtc
    25561 aactgatcaa actgatgatt tttaatattt ggataaaata aaacctcttt taaaaaatct
    25621 accgcttgag aaaaaaggtt tggatcattg acatattcac cattcacaaa actgatccct
    25681 gcgtttagcc aatgtaaatt tccttttttt ctaactgata atccaaagct tgcgccataa
    25741 agctctgcaa gttgcgcact taactttgtc tgatccggat aattcaaact atttgtttcc
    25801 aacatgctgc tgagaagtga acgtttcgtg atgacttctt gttgcagacg cgcagtaaaa
    25861 cggataaaaa tccggatcgt tttgtattgt tttgttggta agacatgaag attcacgcct
    25921 tctgccaatt gatacaccat atttatatgc tccttttcac agttgattgc ccgcttactc
    25981 ttttaatggg tcaacctgcc ttttttactt attcctctgc ttgaaattta taagttcagg
    26041 caattcgccg ttttttctaa atgatgttac actttatagt atagcataga tgttttttat
    26101 caaaagaaac agacggctaa aagcgtatat tgttttttta ttttttattt ttcgatatac
    26161 taaagacaaa taaaagaagg gagggtcatt catgatcaaa gaattcaaag agttcatcat
    26221 gcgtggagat gtcttggatc ttgctgtcgg cgttgttatt ggtagtgcgt ttacaagcat
    26281 cgtaaaccaa gtggtgcaag gcttgatcac accactaata ggttggtttg tcgcaatgat
    26341 cacaggtact tctgatttag aaggagcact ttcaattctt gactggtcgc cgacaaaagg
    26401 cgtcacattt gcgtttggta acgttattag tgctatcatt acatttctga tcactggttt
    26461 tgtcttattc ttagttgtca aagcggcaaa ccgtgcgaag aatttacgct ccaaagaaga
    26521 gcaagtggaa gtggaagaaa ctcctactgc agaagattat cttagtgaca tacgcgatct
    26581 tcttgccaaa caagtggcag aagacaatac acatccagta gagcaaacaa atctttccga
    26641 tacttctaaa taataaagaa tctaaaaaaa agaaagccga ttttcagtgt cggctttctt
    26701 tttttagatt cttaacttgc tacttgttcc tttttcatgt cgatatgtgg aatatcatct
    26761 tccaaataca catctgagat tgccttgaat ccaaacgact cataaaaagc ttgtaggtat
    26821 tcttgtgcgc cgatcacgat tggtttttct ggaaaatttt tctggatcat gttaatcgtt
    26881 tcttgaacaa tctttcttcc taaaccatct ttacggttgt cctgatgaac aaggacacgt
    26941 ccaaaatgga tactctccgc ttcttcatat actcgcgtgt aggcaaggat cttcccatca
    27001 gtatcttgaa aaaacgtatg ccaagcttgt ccatcaatgt catcaacttc ttgataagga
    27061 cactgttgtt ccacaacaaa aactgcgatt cgttctttga cgatcgcgta aaactctgct
    27121 gttgacaatt cttcaaattt ctttacctga tattccacta agcaaacgcc ccctgctttt
    27181 ttattcttag cataccgatt cgcacacaaa aaagcaaacg aaccttacga atctcaggct
    27241 cgtttgcttt ttaaactgct tcagttaaaa attcgatctc aactgtccga gtaagtacat
    27301 ctgaatagct gtaagatact cgttcaaaag aattttcttc aggatccaaa tcaacaacga
    27361 atactgaagg ataggtttct gttaaaacgc cgcggcgttc tgtttgacgc tttcttcctg
    27421 tctgagcaac caacatgatt gggctgccga tacgaccttc taaatctttc ttgattgaag
    27481 ctaaagttgt tggcatacag ttcacctcta ctatcaacta taccacatat ttttaaaatt
    27541 ttcaagttta ccataaaaaa aaaagaaatg caataaaaat aaataagaag ctcattaaga
    27601 tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg agttttactc
    27661 tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc atgtctacaa
    27721 aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa aactggattt
    27781 cgcaattttc gattgcttct tttaataaaa gatgctgaat cgctaaaggt tcttgcaatg
    27841 tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt gagacttcat
    27901 ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag atttgattgc
    27961 ttataatgct tttataataa cttccaaagc taacaccttt gcttttttcg taagttctca
    28021 atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca taatttctta
    28081 aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac agggcagcaa
    28141 atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata aaataattcc
    28201 tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact gattagaacg
    28261 atatgttatt tattttttct cattaaatcg tccaattttt ggataaagtc cgtataattg
    28321 tgtaaaagta aaaaggccat ataacagtcc ttttacggta caatgttttt aacgacaaaa
    28381 acatacccag gaggactttt acatgaccca agtacatttt acactgaaaa gcgaagagat
    28441 tcaaagcatt attgaatatt ctgtaaagga tgacgtttct aaaaatattt taacaacggt
    28501 atttaatcaa ctaatggaaa atcaacgaac agaatatatt caagcaaaag aatatgaacg
    28561 aacagaaaac cgacaaagtc aacgaaatgg ctattatgag cgcagcttta cgacacgtgt
    28621 aggcacgcta gaattaaaag tacccagaac acgtgatggc catttttcac ccacagtgtt
    28681 tgaacgttat caacgaaacg aaaaagccct catggcttca atgttggaaa tgtatgtatc
    28741 aggcgtttca actcgtaaag tatcaaaaat tgtggaagaa ctttgtggta aatccgtctc
    28801 taagtccttc gtttctagct taacagaaca gctagaacct atggttaacg agtggcagaa
    28861 tcgtttatta tcagaaaaaa attatcctta cttaatgacc gatgtactct atataaaagt
    28921 acgagaagaa aatcgagtac tctcaaaaag ctgtcatata gcgattggaa taaccaaaga
    28981 tggcgaccgt gaaattatcg gcttcatgat tcaaagtggc gaaagcgaag agacctggac
    29041 aacatttttt gaatacctaa aagaacgcgg tttacaaggt acggaactcg ttatttctga
    29101 tgcgcacaaa ggattagtct ctgccattag aaaatccttc accaacgtaa gttggcaaag
    29161 atgccaagtt cacttcctaa gaaatatctt taccaccatt cctaaaaaaa attcaaaatc
    29221 tttcagagaa gctgttaaag gaatttttaa gttcacagat attaacttag cgcgtgaggc
    29281 taaaaatcga ttgattcatg attatatcga tcaaccaaaa tattcaaaag cttgcgcatc
    29341 attggatgat ggattcgaag acgcctttca atataccgta caaggaaatt cccacaatcg
    29401 actaaagagt accaatctaa ttgaacgact gaatcaagaa gtacgcagaa gagaaaagat
    29461 tattcgcatc ttccccaatc aaacatcagc caatcgctta attggagccg ttcttatgga
    29521 cctacatgat gaatggattt attcttcaag aaaatacatc aattttgata agtagaaatg
    29581 gtaaaaacat tgtatagcat tttacacagg agtctggact tgactaattt ttgggacaat
    29641 ttttttaact gctcttcatt ccatggagaa ttacgtcgga aatcaataaa atgcatatct
    29701 ttattttctt gggctattgt tttttccgtt ttttttatcg tcttatataa ttcataagca
    29761 gaagttcgta atgctccttg ggaaaagatc atccactgtt ctgctaaatc gcttgtcgcc
    29821 actgtcactt gtgtcaaacg gtcattcagc tgaccagcga ttctctcgat ataactgtca
    29881 gccgtttcgt cttctttcgt aaaaacaaca tccaactggt atttcttata attttgttgg
    29941 atgcccggaa ctagttgggc atcgaagaca acgatgactt ccattccttc atatttcgca
    30001 taatttgata aacgatgtaa aagagcttct cttgcatccg ccattttgtc ctgcttttta
    30061 agctggacaa gttccggcca cgaaccaatc atgttgtagc cgtctacaat caacagctgt
    30121 tttttcatgg tctactcccc ctgacgttgg gaaaaagcct gatacataag caaaccagca
    30181 gcaacactgg catttaagct ctggacgtgc ccggtcatcg ggatcgtcag caattcatcc
    30241 atttcttttt tcaatcctgg actcattcct ttgccttcat taccaatgac taaagcaatt
    30301 gatcctttgg cattccattt ttggaagttt gtgccggtca tatctgtgcc aaatacccaa
    30361 aatcctgttt ctttgagctg ttgcacagct tgtaccaaat tcgtcacgcg tgcaacaggg
    30421 acatgttcga ctgccccagt ggaagtcttt gttacgatag gtgtgattcc aactgctcgg
    30481 tgttttggta tgatcacacc atcgacgcca cttgcgtctg ctgtccgtaa aatcgaccca
    30541 aaattgtgtg gatcttcgat gctatccaaa atcaaaaaga aggggtcttc ttttttctct
    30601 tttgttcgtt caatcagttg gtcaagggat aaatattcat aaggtgtaat cgcaagaacg
    30661 actccttgat gcacgccgtg atcgctcaaa gtttctaatt tttgtttagg aacccatttg
    30721 actggcacag cttgttcacg tgcgatttct ttcagttctt ctactttttc tcctcgtgaa
    30781 tcttcttgaa gaaagagttt attccctcgc ccttgttgta gcgcttctac tgcggcatgg
    30841 tgcccaaaga cgaaattatc ttctaatgct tcttcatttt gatcagaaac tgactgattt
    30901 cgtccagttt gctttttatt tgaatatcta ttttgttttt tgtcttcaaa tccacgtttc
    30961 gattgatgtt tgtttgttct ttctttaccc atttttttca ccaactttct gaatacacca
    31021 gcggatcaat tcttccatcc gctcggtttg ttttgtcaaa tgcagatagc ccatgagcgc
    31081 ttcgaaccct gttgcgaccc gatacgtcgt aacatcagca ttttttgcgc tcgtatggct
    31141 tttagcattt cttcctctgc gataataaag ttcttcttct tctgtcaaaa gatgttcttc
    31201 cagcatttgc tgaatcaaaa aggcttgtgc attcgcagaa acataattcg ttgccgcacg
    31261 atgcagctta ttgggttttg tttctccttg atcaatcagg tggtcacgaa catagatttc
    31321 gtaaatcgca tctccaacat aagccaaggc taaaccgttc aattgtgtgt aatctctcat
    31381 gcttctcttc tccatcgggt tccttgcggt gtatcttcta ataagatccc tttttctttc
    31441 aacaaatcac gaatttcgtc gcttcggaca aaatcacgat tgcttcttgc ttgatttcgt
    31501 tcttcgatca attgttcaat ctcttcatct aataactctt ctgctgtaaa taaaataccg
    31561 aagatcgaaa gccattgcga gaataaagct tctccttgtg ccaagacagc ggcagaaact
    31621 ttcggctggt ctagataagt gttcagccac ttagcaagct catagaccac tgtgatccca
    31681 tttgctgcat taaaatcatc gtccatttct tcgatgaatc gctgttctaa attttctaac
    31741 aaagcgattt tatccgcatc atctgccaaa ttctctgagg cagtttcttg gcggaatttc
    31801 aaattgtcaa acgtattctt cagtcgttgt aaattggtgg ctgcttcttt catcgtcgtt
    31861 tcactgtagc ggatcggacg acggtattgt gtggtcgcca tgaagaaacg caagacttgt
    31921 ggatcaacgt ttttgatcat ttcatgtaca gtgatgaaat tccctagaga tttactcatt
    31981 ttttcatcgt cttcgccaat cgttacatag ccattgtgca tccagtaatt agcgaatttt
    32041 ttccctgttt tcgcttcact ttgtgcgatt tcattttcat gatgaggaaa ttctaaatct
    32101 tgcccgccac catggatatc gatcgtttct cctaaatgtt ttgttgccat aacggaacat
    32161 tcaatatgcc aaccgggacg tcctttgccc catggtgaat cccaagaaat ctcgccaggt
    32221 tttgcttgtt tccataaagc gaaatctaat gggtcttctt tttgctgctg ttcttcccct
    32281 gtacgctgac ttgctccgac ttccagttca tcgatggatt ggtcacttaa tttcccataa
    32341 ttttcaaatt tacgggtacg gtaatacaca tcacctgaag attcataagc atagcctttt
    32401 tcaaccaaca cttgaataaa tgctaaaata tctggcatat gatccatgac tcttggatgc
    32461 agtgtagcag gctcgacatt caacgctttc gtatcttctt caaatgctcg gatgaaacga
    32521 tcagcaactt ctggtgctgt aatacctaat tcgtttgctg tacggatgat tttatcgtct
    32581 acatccgtga aattcgatac gaaatcgact ttatagcctc gatattcaaa atagcgtcga
    32641 attgtatcga aagcaatggt gctacgagca tttccgatat ggatataatt atacacagtc
    32701 ggtccgcaga catacatacc gacatgtccc ttttgtatag ggatgaattc ttcttttttt
    32761 ctggtcaatg tattgtagat ttttatcatt ctgatctcct cactttttct cctttgattc
    32821 gaacgacttt tgctggtact cctaccgctg tagcgtcgtc tggaacatct ttcaacacaa
    32881 cggcacctgc accgattttg gcgttttttc cgatcgttac cggtcctaat atctgcgcat
    32941 tcgcagagag catggctcct tgtttaacag ttgggtgtct tttacccgtt tcttttcctg
    33001 tgccgcctaa tgttacgcca tggaataaaa tcacatcgtc ctcgatttct gcggtttcgc
    33061 caatgacgat tcccattcca tggtcgatga acacgccagt tccaatcgtt gctcctggat
    33121 ggatctcaac accagttagg aaacgccaga attgagcatt gatcttcgcc aataaaaaga
    33181 gccggtgctg atataaaaaa tgagagaaac gatgccaaaa caaggcatga aaaccagggt
    33241 atgtcaaaac gatctcagct gtcgaacgtg cagccggatc gtttctcttt gctgcatcaa
    33301 tcgctcgggt aaaccaactc atgactttcc actcctttcg taagaatttt atgtaggaaa
    33361 tcatcttctt ataaagaaaa gaagcgcctt ttaaataagt ttccttatca aaagacgctt
    33421 tccgcgtggt tccacttttc ttcataatca atgattatct ctgacagcta acgcctgtta
    33481 tacgttcttg gctactttgt ttcctataaa acagtttttc ctgttttctg tgtttcgcca
    33541 agaccactcc cagaggcatt tcggcaacca ttcattgata ccttgcacca cccggtatct
    33601 ctcttgtaat gatcagctgc ttactttttt ctgttcatcg tgtttagttc agtacttgat
    33661 ttaaatgtgc taatgatttt tcacgaccaa gcaattcgat cgtttcaggc aattctggac
    33721 catgcatttg tcctgataca gctacacgga ttggcatgaa aagatttttt ccttttacgc
    33781 cagtttcttt ttgaacagct ttgatggcag ctttgacagt tggagcatct actacttcca
    33841 tttgttctag ctgtgctttg aatgcgtgga gaacagtagg aactgtttca ccagcaagta
    33901 cttcttttgc tgcttcattc aatacaggat gttcattgaa aaatagcgta gacagatcaa
    33961 cgatttctgc agcatagctc atttgaggtt gatacaggct aacgatcttc ttcacccatt
    34021 cgattgtctt ttcatctggg ttttctgcta cacgtccatc agcgattaga taaggcagac
    34081 acatgtcagt aaatgcatct agatccattg ctttgatgta atggttgttt acccattgca
    34141 gttttttcgc atcgaaagct gcgggtgatt tacttaggcg ttttggatcg aacattttga
    34201 tcaattcttg gtgttcgaag atttcgtctt cgcccacagg tgaccagccc aataatgcaa
    34261 taaagttgaa cattgcatct ggtagatagc ctaattcgcg atattgttcg ataaattgca
    34321 agattgattc gtcacgtttg cttagttttt tacctgtttc actgttgatg atcaatgtca
    34381 tatgtccgaa ggttggtggt gtccagccaa atgcttcata gatcatcagt tgttttggtg
    34441 tattggcaat atggtcatct ccacgtaaaa cgtgagtgat tttcatcaaa tgatcatcga
    34501 ccgcaacagc aaaattataa gttggcatac catcgcgttt ttgtatgaca aagtcgccac
    34561 cgatattatc ggattcgaac gaaatagcgc ctttcaccat atctgtaaat gcgtattctg
    34621 tattgcgagg cacacggaaa cgtacaacag attctagacc ttgtgcttct ttttctgctt
    34681 gttcttcagg tgtcaagttc gcacatgttc ctgcgtaatg aggcatttct cctcttgcgc
    34741 gttgtgcttc ccgttctgct tctaattctt cttctgtgca atagcatttg tatgctcgat
    34801 tgctagcaag cagctgatca atcagcggtt gatagatttc tttacgttca gattgacggt
    34861 aagggccgta ttctccagga ttttcaggag attcatccca attcattccc aaccaagcaa
    34921 ggttttccaa ctggcttttt tcgccatctt cgatattacg tttttggtca gtatcctcaa
    34981 tacgaataat aaagtctcca tcattgtggc gggcaaacaa ataattgaat aatgcagttc
    35041 ttgcatttcc aatatgcagg tgcccagttg gacttggtgc atagcgtacg cgtactttcg
    35101 tcattttctt cctcttcttt caaactagac tttgactaac agcaaaaatt ccgtcagtta
    35161 cggcttctca aatatcattt atttaaagcc actcagcaca aattttacct tttctaaggt
    35221 aaatagtaaa gccaactggt tgaatttcct taccagttgg ctgaatttct tttttatttt
    35281 gattgtttag tctttttctc tgtctctttt ttattgtcga ttcctcgtcc tgcatgtgca
    35341 ggtttagcaa agatcatgcg tccagcagct gtttgaaggg cacttgttac aacgacttcg
    35401 atacgttcat tcatgtaatg ctgtccatct tctacgacaa ccatcgtgcc gtcatcaagg
    35461 taggctactc cttgttggcg ttctgtcccg gcttttacta ctagaacatt catcgtttca
    35521 ccagggatga cgactggttt tacggcattt gccaacgcat tgatattaag aacaggtaca
    35581 ttttggaatt cagagacttt attcaagtta tagtcattgg ttacaatgac accatctaaa
    35641 agcttagcca atttgatcaa tttgctgtct acttcactga tatcttcaaa gtctccatcg
    35701 tacatttcga cagatatacc ctcttctttt tgcaaggcat ttaggatatc taatccgcga
    35761 cgaccacgca cacgtttcaa actatcccct gagtccgcga tgtattgtag ttcgtaaaga
    35821 acaaaattag ggatcatcaa ggttccttct aagaatccag tctttgtaat atcataaatc
    35881 cgaccgtcaa tgatcacgct tgtatccaag attttatatt tatggaaatt ttctcctgat
    35941 tttcgttcta acagttgact ctcaacttga ctagctgttt cttgctcgat ttttttgctt
    36001 cgtgaaccaa aaactttctt ccattcttcg attcgtgtcg ttcccattct caatcccaga
    36061 tagcctaaga tgatcatcag taaaatcggc aatacactat tgacgaatgg aatttccata
    36121 ttgtaaagag gggtcgagat cacaacacct aaagtcaatc cgatgatcgc accgatcgca
    36181 ccgaagagaa gataagtcaa gctgacctca cttagttttt gttctacttg tttgataata
    36241 gctgagatat gtttggccaa cagcaaagat aataaaaaga aaataagtgc gcctaacagg
    36301 ctgttggtaa atgtattatt cagccatccg tttgactgtt gaccgattgc ctgccaagct
    36361 gagggtaaaa atgaaatacc caagcttgcg ccgacaatga tcatcagcaa agtaatgaca
    36421 cgtttttgca tcgtttttcc tccgtttcga caatcattta ttagttagcg gaacacacgc
    36481 ttcaacgttt cagccaaagt tgctactcca actatctcga ttccttttgg cgcttcccag
    36541 ttacctaagt tgtttttagg taggtatact tttgtgaagc cgagtttttg gacttctttg
    36601 actcgttgtt caatatgact gacacgtctg atttctccag tcagtccaat ttcaccgatg
    36661 aaacattccg ttggctgtgt tcctttttct ttatagcttg aagcaatact gacagcgata
    36721 gctaaatcaa tcgctggttc atttaatttt accccacctg cagcttttaa gtaagcatct
    36781 tgattctgaa gaagcaaccc agctcttttt tccagcactg ccatgatcag agatacccga
    36841 ttgaaatcaa gacctgttgt tgttcgtttg gcatttccga acatagtagg tgtgactaat
    36901 gcttggatct ctaccaaaat tggacgtgtc ccttccattg ccacaacgat agcagagcca
    36961 gttgcaccgt ctaggcgttc ttctaagaaa atctgtgaag gattagcgac ttcttccaat
    37021 ccatgctcat gcatttcaaa aatcccaatc tcattcgttg agccaaatcg gttttttact
    37081 gctcgcaaaa tccgaaaact atgatgttgt tcaccttcaa aatataacac cgtatccacc
    37141 atatgttcta acattcttgg gccggcgatc gagccttctt tggtaacatg accgacgata
    37201 aaaatggcta tcccatttgt tttagcgatt ttgagtagtt ctgcagtggt ttctcttact
    37261 tggctgacac ttcctgccac acttgtgatg tctggctgcg tcatcgtttg gatggaatca
    37321 atgataacat aatcagggga gatatgttcg atggcacgac tgatttcgtt catatctgtt
    37381 tcagcgtata gataaaattc tgtgttgata cttccgaggc gttccgcgcg taattttatc
    37441 tgctctgcgc tttcttcgcc ggatacatat aagacttttc cgcctatggc tgctaattgc
    37501 tgagaaactt gtaacagcaa agtggatttc ccgatacctg gatcgccacc aatcaagacc
    37561 atagaccctg gcaccacacc tccgcctaac acacggttca gttcttctaa ttttgttttg
    37621 atccgaggtt cttttttagg gacgacgtct gcgatttttg ttggttttgc tttttctcct
    37681 gtcaaacttg tccgagttct gcggtccgtt gtatcttgtt cgatttcttc gaccatcgta
    37741 ttccattttc cgcaatttgg gcagcgtccc aagaacttag gcgacacata cccgcaattt
    37801 tggcaaacaa attgtacttt tgattttttt gccattgtac cgcctccttt tatcctaatc
    37861 atgagacatt aaatcaagct taagtatctt taacgtttta tttactggaa gaaccaaatc
    37921 cgccttgacg ttgagcagtc gtttgatctt gatctgcttt taaaaacggc aaaaagatcc
    37981 cttggccgat acgctcgcct tttttgatca ctacatcctt gaaaccaaag ttggtgaatt
    38041 ggaacatgat atggccttca tttgcctcat tattgtaata atcactgtca atcacaccta
    38101 ctccgtttgt caacactaaa aaccgcttca acggattgct tgaacggtta gcaagctgca
    38161 aaaattcatc ttctcccatg taagctttga ttccagtagg cacgagggtt ggtttcaatt
    38221 gtttttcatt ttctgataca tcttttttca atccatctgc gactgtttcc aacccctttt
    38281 tccagcctgc tttccagata cttggaatca cgacctcttc tgctgcttca aaatcatagc
    38341 cagcagcatg atgtgtagcg cgctgcggta gattgatatt ttgatcttta tatttagaaa
    38401 taacttcaaa tcctcttttt ctcaccggtt ttcctcccta aataaactac tcctattcta
    38461 acatatccga ggctgtaaaa gactttcttt acaaattctt caagaaattc tgctatctta
    38521 aagtaaacca ttaattcgtt ctttttccaa acgactgcaa gttgtacaaa aaaactattt
    38581 tttacatgac tttactcgtc aaaacaagta gaatagaacg aaattaatga gtggatattt
    38641 gattgaggtg tcacatgcgt aaagaaatta aaaatgcaaa acgaattatt gtcaaagtag
    38701 gaacaagtac attgatttat cccaatggaa atatcaatct tgcggcaatc gatcagttag
    38761 cttttgtcct ttctgatctt agaaatcggg gaaaggaagt gatcttagtc tcatcaggag
    38821 ctgtaggcgt tggtatgcat aaattacatt tgtcagaacg tccgactttg attcctgaac
    38881 agcaagctgc ggcggctgtt ggtcaagccg aactgatgaa tatctataat caaagattca
    38941 gtgtatataa tcaacagacg gcacagatcc tcgtgacaag agacgtggta gattatcctg
    39001 aaagtcgtaa aaatgtgagg aatacattgg aacagctttt gattatggac acgatcccta
    39061 tcgtgaacga aaacgatact gtagcagtgg atgaattaga tcatctgaca aaatttggcg
    39121 ataatgatca gctgtcagcc atcatggcaa aacttgtcgc ggcagacctt ttgatcgtcc
    39181 tttctgatat cgacgggttt tattcagata atccgactac aaatcccgag gctcagatgt
    39241 atcgctatat cgataaaatc gatgaaaaga ttctggcaca agcaggtggt gctggtagca
    39301 catttggaac aggcggtatg cagagtaaat taaaagccgc ttctaggata tttgaagcaa
    39361 acagcgcaat gattttagca aatggaaaaa atccggcaat tatttttgat attcttgcag
    39421 gaaaagaagt aggtacttta tttaaggagg acttgaaatg actgacttga tgcaattagg
    39481 acaacaggca aaagatagcg cgcaacaact agcacttatg cctacaaacc gcaaaaatga
    39541 gctacttgtt caaatggcac aaaccatcaa aaagaatcaa caagcgatca ttgaagcaaa
    39601 tcataaagac ttagaaaaag cagtcgaaaa caatatcagt gaaacgatgc aagaccgctt
    39661 gcagctgaca cctgagcgaa tcaatgccat ggctacagag atcgaaaaaa ttgcttcgtt
    39721 agacgatcct attggaaaag tagatgagat gtggacgaac accgacggat tgagaatcgg
    39781 caaaaagcgc gtgccactag gcgtgatcgg catcatttac gaatctcgtc caaatgtcac
    39841 gacagatgca gctagtctag cattcaaatc agggaatgcc gttatccttc gcggtggcaa
    39901 agaagcattc ttctcgaatc aattattagt ccaattattg cagcaagtac tggtcagtgc
    39961 tggcgaatct ccttatgcca tccagtttgt cgatgatact tctcatgaaa ctgcaggaca
    40021 gttgatgcga ttgacagaat acttagatgt gctgattcct cgaggcggag caaaattaat
    40081 ccaacgagtc aaagaacagg caacggtacc tatcattgaa acaggtacag gaaataatca
    40141 cgtatatatc gataaagaag cacagttatc tatggcagtg aacatcatcg tcaatgcaaa
    40201 agcttcccgt ccatctgtct gtaacgcggc agaaacattg ctgatccatt ctgagatcgc
    40261 accttttttc ttgccagcta ttgaaaaaga actggtagaa cacggggtct ctttacgtgc
    40321 ggatgctcgt gcgttagaat atctagagac ggctgcttta gcagaagaca cagattggga
    40381 tactgaatat ctagattata tccttgctgt caaagtggtc gattctttgg atgaagcaat
    40441 tgcacatatc aatcggcata atactaaaca ttcagaaacg atcgtgacag atagttatgc
    40501 ggctagtcaa cgtttcttaa atgaagtgga cgcggcggct gtctatgtca atgcctccac
    40561 tcgttttaca gacggatcgg tatttggttt tggtgcagag attggtatct ctactcaaaa
    40621 gttgcatgcc cgtggtccaa tggggttaaa tgaattaact tccacaaaat atatcattta
    40681 tggcgatgga caaatccgcg aataatgttt cgtgtgaaac attattcccg ccttttgcct
    40741 tttttctgta aaggttctat ccaactagaa aggtgtcaat atgtttgaaa ttttaatgat
    40801 tttttttatt taccttattt ctctgaatat cgctgctttt ctgggagtcg gtattttatc
    40861 ccttttcttt caattcaaaa aacgaagcat agggagtcaa cgggaaaaat ggtcgcagta
    40921 ttttgacaaa atcgggcaaa aagggctggt tacacgcttg cacattagtt acatggttgc
    40981 attatgctta ttagcgacaa tcaattacta ttcttttttc gatcattcaa tcgcttatac
    41041 gattacgcta ctgattgctg ggatttttca cttatcttat aaatatcagc tgaataaaaa
    41101 ccacttgaat cggactttcc gctaaaaaat atccgtacga taagtagagt gccttgaaga
    41161 tcggcaaaaa actgccggac tttttagagg ctgtgacaaa gattttgtca cagcctcttt
    41221 ttccatataa acggcgttcg cctaccagct gcctggtaaa tctgtttcct cttcgttaag
    41281 aagcgaaaat ccgtcaaatg tttcgccatc ataataacga agcagctcat ccatggacaa
    41341 gttcatcgtt ttttcttttt cttcttccgc tgattcagcc agtagctgtt cccgatacac
    41401 ctgatacata ccagctattt tttgctgcat cgcttttcta gtttcacctt ccgcattgat
    41461 tattggcaat tctttgatat agccacgata aatcaaccga tcatctgaaa attcaatggg
    41521 taattcaacg agtgtcaacc cgacttcctg aaaatattct tccagcatct attatccccc
    41581 cattcttttt cttaaatgaa ttttagctta cctactgaaa attttccagt ttcattattt
    41641 tgagaaaaaa agggaatgtg acaaaaatcg tgtcacatcc cctttttccg aataaatggt
    41701 gttcttctgc caactcctca atcataagtc acaatattca acaaacatga gaaactgttt
    41761 tttgaaattg ttccttgtga ttcagagtta aacgacagtc tcacaacctc ttggtaaacg
    41821 gcgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata tgagaagcta
    41881 tttttgtttg gctgttctta tgacttgatg caggacagct ttttcacaac ctctgatttc
    41941 ttattcttct tcatttgtag caaaataatt acggtgataa atgatacaac atacactgat
    42001 aagaacaatg gcacctgcaa aataaaacgc atagctttca ccaatatgtt caccaatcac
    42061 acctgcaata aatggtgcta aagctccacc gaaaaagcgg acaaaactat agctggacga
    42121 agcaacgttt cgttgcagac ctggtatttc catagcaatc gtcgtcaaca gtgtattgat
    42181 aattccttgg aaaaatcctg ctaacacaat gctgatcggt acaaggactg gcatagaagt
    42241 tccagctcct aataacagca aacaaataat gaataagaca aatgctgcta aaattgtccg
    42301 ataagtcgta aacttccgct caagagccgg tgcaacgaaa atcgaagcaa tagccagaag
    42361 cacaccccag ccaaaaaata caaaaccgac ttctaattca gaatagccaa tcaataaaaa
    42421 tggcgcatac gcaagtaaag taaagaatcc gaaattatat aacaatgcga ttagtccaac
    42481 tgaacgaagt ttatgattgc ttaacgcttt gacaccttcc caaaggccta cttttctctt
    42541 tggtttctca gtttcaggta aaagtaccgc tagggctata aaaccgcaaa acatcaaaaa
    42601 tgctacccca aaaaatggat aacgccatga accactgccc aagaccccac cgactagagg
    42661 gccaacagcc attccgcagc caagtgctgc ttcatacatc atgattgctt tttctgtgtt
    42721 accaactaaa atactgacga tcgttgctaa agcagtagag acaaataaag cattcccgac
    42781 accccagccg gcacgtaacc caatcagcat tccgatattc ccagataatc cgccaagacc
    42841 agcgaataca acgatgataa acaatcctgt catcaatgtt ttttcaaacc gatccgacta
    42901 gataagaaac ctgtaaatag catcaccaca ccagtgacta acatgtaact agtaaataac
    42961 agggtcgttt cagctggcgt agcgtttaac tgtgcagcaa tcgtttttaa gattgggtct
    43021 actagaccca ctcccataaa agcgatcata caggtagctg ctacaactag cgctgaactt
    43081 ttcccctttg tttcttcatt tgtttgttct tgtatatcta tttgattctc caatatttcc
    43141 atccctttct ctctattagc atcatacatg atttatttta catgtaaata tgcacatgta
    43201 aaacgtggaa actttactgt gatataataa agaaaataag acgagaggta ggatttttat
    43261 gaatacttta gcctatatat tattatgcgt gcttttatcc aaaccacgtt ctgggtatga
    43321 attgaagcag ctgatcacaa ttttttggga agcccatcac agtcagatct atacaacgct
    43381 tgccaagtta gaccagcagg gttatgtgac cgttttggac aaagacgagc actctcaaaa
    43441 aaagatctac cacttgactg atgaaggacg tgctcttgta gaggaatgga tcaaagagga
    43501 aactcccgct cctacgcaaa aagatgaatt tctagcaaaa atctatgctt tttcgacgct
    43561 cgataaaaca acagctactg gactgctatt cactagagaa caacagctga acaaaatcat
    43621 gcaaaaaaat caaaaaaaat tgaatgacct ctcgctagac aagaaacaag aatttggacg
    43681 ttatgttgtg atccagcgca gaattatgct ttgtcagcag gaactccaat ggtgtcagca
    43741 agtaagagaa caaatgcagc agttttttga gtaagggata aacagaacct ttctagaaat
    43801 atctattgaa gagcaaatta tcttcaataa aagagcaata cagttggaaa agaaaaagcg
    43861 atccgaacga caggccaaaa cctatcgttc ggatcactat ttttctagaa ctgcattcaa
    43921 gagactatga caaaaagcat gccaaggcct tttttcaaat aacagtgttc catcattcat
    43981 tactttgaat tgcttttagc acacttcttg aaagcgggcc aacgatgatc aactgcaacg
    44041 gtaaagcggc aatcacattc attttccaag cattcatata agcagagaat aattgatcag
    44101 gtattccttg ttccatgatc acaccgaata aagacataca tgtcaccatt cctaaaacca
    44161 tcaagcatga aatagttaag atcatttgga ccttgctttc tggatttact ggtagtttaa
    44221 aggcaatttt tttcgccagc acaccgatga tgaagacatc caaaatgaaa gccaccacga
    44281 atccaggcac tagaccaatc aacaaacttg ataaatgaag atcttgatgc agccataaat
    44341 tatacgcgct catccccaag accatcaaaa aacacatcat tgttgtaaaa attattcctt
    44401 cttttttatt tgtaggcaat ctgtaccact cctttaaatt tgtcacgaaa atcatcgtag
    44461 cacatttaaa aatttctcgt aagtactttt tttattttct gcccttttat ttcataaact
    44521 tcatcagccg tacgctcgac aaagtaagaa tcatgagaag taaacaaaat cgttcccgga
    44581 tatatttgca ttagcttctc taaagcttcg atcgttgcca tatcaataaa attagtcggt
    44641 tcatctaaca gcaacacatt acttggctta gtaaaaagca aagcaatcgt cagacgggtc
    44701 gcttctcccc cacttaatgt acataacggt ttcgtcaaag tttcctgagc aaaccctaag
    44761 tgattcagca aactacggac aagcgattct gaaaactctg tttgtctcat gacaaaacga
    44821 atgatggatt cttcagatgt catttgataa cccttttgct gatatacttg aaagcttact
    44881 ttcggagaga gaaggattcc ttctccttgt ttgcggatat gttctaataa ggttgtctta
    44941 cctgaaccat tttcaccgac aatcgcgatt tttttaccta aagaaaactg aaaatcacca
    45001 tttaccagta atgttctccc acttctttcc aattggacat tttgtcccat gatgggatag
    45061 cggctgtgga gttcaagaga ttttggtact ggaaaacgga tttgtttcat ctgctgcggc
    45121 ttggttgttt ctcctatttt ttggagtctt ctctctaatg ttttcgcatt cttttggatg
    45181 gctttttgta cactgtcttt ttgtttagag gaagacaacc gatccggtct gatttgttgc
    45241 tttttctttt tactggacac tttttccaac ttttccgctt gttttttctt ttcttggatc
    45301 gatttttcca atcgtttttt ctctttcatg atcgactctg cttctcgtag ctgagtcttt
    45361 ttctccaatt ccttttgagt gagataggca ctataattcc ccgaaaactc tcggatttct
    45421 ccgtctttta cttcccagat tttcgatgct agctcatcta gaaaatagcg atcatgacta
    45481 acaaaaacca gcgtaccata ataataacga agctcttcaa tcagtcgatc aatacttttt
    45541 ctatctagat gggttgttgg ctcgtctaga agcatccctt gttcataaga agaaagaaga
    45601 cgtatcaact cgatctttcg ttcttcccct ccgctccatt tccgctctga agtttcttcc
    45661 agtcttaact ggaaatgact ggctaattcc aaatttaggt cctttgcttc agcgagatag
    45721 gttaattgag gcaggtagtt gaaggtgatc tctgtttgga ttttcccatg atcgggaaaa
    45781 agttcaccgg ctattagttt gagcaaagtc gatttaccga caccattttc tcctatgatc
    45841 cctatacgat cttgctgata gacacgaaga gaatcaatag atagttctag ctggttgcca
    45901 aagctgactt gtagatttgt tatatttact gctaaatttt ccatatgttt tacactccta
    45961 tttcatgtaa aacaacaatt atcgatacta aaaaagagca gaaaaatctg tttgcaaaaa
    46021 aactgcatca catcttttcc tactctgaac gaaataacta tgactaaatc accaaatctg
    46081 tcatagcttt cacctattct actgtcgttc gaaaaaatgg acatactcca tccatgaaat
    46141 tgttgtttat acatgtcttt tgaatttatt tagttgttat tcaaaagttc gaaacgtaat
    46201 ttcatcgatg cagtcatttt tccgacctcc ttttttatta ggttaccaca cacgattcaa
    46261 aaaaagcaat agctactgaa tagtaactga aaacctttta ttcgaacgta gtgatctttt
    46321 ctggtgctaa acgaacagac tcgtaacctt cttctaccgc ttttcctatt gataaaataa
    46381 taactggaac ataacggtct ttatccaaac caaatgcctc tgccaactga tctgcttcga
    46441 atccaccaat tggatttgtt tcatagccat gatcacgcgc aactaacatg aattgcatag
    46501 ccgctaagct agaatcgact ttgactacat cgttcatttc ttctcttgaa aaactcttgt
    46561 aataaggaat gatcgcagct aattgttgat ctctgacttc ttttggcatc ttccctgatt
    46621 cgtaagcttg gttatagatt tcttcgccat attcgtaaca ttgcatatcg ccaaaaatca
    46681 tgaccattgc agaagaagta tcattttgtc tcgtattgaa acggatcaaa ggacgcaaga
    46741 tttcttttgc ttcctcgctt tcagcgacca caaagcgcca tggttgcata ttgacagaag
    46801 aaggtgcagt cgtcgcttct ttgatcattt ccagcatttc ttcgtgtggg attttgtatg
    46861 tttcatcata tacgcgaacg gatcttctac cagtcataat tgttgaaaaa tcattttttt
    46921 gttccattat tattaacctc tttcgtttct gttgttatat tgcaaagttg ggtattcaat
    46981 ttatctaaaa gtgttagaaa ttgtttcatt tctacatcgc tgaaagccgg ggatagcacg
    47041 gaacctgtat ctttatggtc catttcacaa tgctcgattt tcttctgggc tttttctgtt
    47101 agcgctacat acacttcccg gttgttctga ggatttctct gacgcgaaac atagccattt
    47161 gcttccaata ccttcagatg acgagtaact gctccttgat caatctgcaa cttttcttgc
    47221 aaactgcttt gtagcaatgg attttcttct ttcagtctca tcatgatctc gtatctagta
    47281 agacttatac cgatattttc ttcaaagatc tggacgattt tttgatcggc taatttgatt
    47341 tgatataaaa ggtgactaat ctcttctaaa gacataattt cctcctttct aataattgac
    47401 tcgtcaacga ttgacttgtc aataatagaa taaaaaaaga aaaaagtcaa agaatcagct
    47461 gtgtaataaa aaaaacgctc tcaatagaaa agtatagaga aaagaaattt tttgtagaat
    47521 gaacggaaaa aatgataaag aaagcacaac gctaaaacca agattattga ttcttatcaa
    47581 actatagaag atagctcttc tgcagtagaa aattttacac cgccaaaaat ttccgaaatc
    47641 gccttttgca agaatcccgt cgaatattag aaattggatc agtatgctta aacagtaata
    47701 aaaaagttat gacaagatgt tgtcacaacc ttttttgagt aaacggtgtt caaaaacttg
    47761 catctgcggt aataagttca accaaacaaa aaacatgaaa atctgttttt gtttggctgt
    47821 tcttactact tgatgcaggg cagctttttc actacctctt tttaataacc ataaaatcct
    47881 attccttcat atctccagcc catcggtgtt agccaatctc tttccttagc actcaaggta
    47941 tagtgatggg taccagtttt ttcatttgga ttaaatagtc gataaattgg cttggaacca
    48001 ccagaataaa aacttagacc ttcataacgc caaccgattt ttgttaaaga gttgatctca
    48061 gattgtgcca ttgtgtaatg atggtctcca ctgtaaggat tgtataaacg gtagacaggt
    48121 tgtccgctgt ttggtgcttg ccaagcaatc ccttcatatt cccagcctac agttggcaga
    48181 taattttttt ctccggcatt caatgtatat aaatgttctc ctgtatgacg gttatacaga
    48241 cgatacatcg gagaagttga agttggtgct gtatcatact gtgtcagatt ataggtttcg
    48301 attacattgt tcaatgtaga accataatta ggcgcagtgg cataacgacc tgttagccaa
    48361 gccgtagcat ctttgtaaga gttcgtattg cttttccatg caccggaata gtagtaaacg
    48421 cctggctgaa aagaagtcgt cttcaatacc gttacgttat cttgcaatga ttctttgtac
    48481 gaaggatatt ttctaaaagc ggcattcacc gtaatccatt gcccattcac atattccgaa
    48541 gtaggcatcg taacagattg gccattatag cttcctttaa tcccaaatag attgtaatta
    48601 ggggcagaag ccaaagtact gttcccccag ccactttcaa cgatggcttg agcaatcatg
    48661 actgatgcat ataagtcatt ggcacttgcc aaactttgtg cagttgaggc aatactatca
    48721 atgaacgctt gttgagaatt agcagatgag cgagcataaa aagcatagcc agtatagttt
    48781 tcgaattcag acaaatctgc ttgatgggta tgtgtcaatg tttgcccatc tgtcaattgg
    48841 ttcgtgattt cttctgcatt ctctggattg ttctgagctt ctgctgatgt actgtcttcg
    48901 gtttgtgttt gttctgttgt ttcagttgtt ggttcttcgg ttgaaattgt attttctgag
    48961 acagtagctg actgcaaagt actctctgta tcgttttcaa ttcctgtttc tgaagaactc
    49021 gatgtctgag ttgcgacagt ttctgtcgat tcttcagcta gcactgttgt gttggcaact
    49081 gctagatttc ctaaaagaat aacgctaaat aataaattct ttgctttcat cttttttctc
    49141 ctttgaatta tgaaactttt cctaaataac agtgtaacta aaaaagtaaa agcaagtcaa
    49201 tgtccatatt taaataacat ttgtttcttt ttgataaatt tgctttttct acataaaaag
    49261 acgtaaatga agaatattct tctccattca cgccttaaat tgatggtcgt ttgaataact
    49321 atttaacaag taattctctt tggatattat ccgttatgga ttatacttcc tgtgtcgtcg
    49381 gacggataat catgttactg acagacatcc ggtctggcgt ttctatcgca aatgctacag
    49441 cagaagctat atcttcggag gtcaatcccc attctttttg cgcttcatga agttcaagag
    49501 cagccttttt gtcgctgatg gtttgatata attcagtttg aacagctcct ggagagatga
    49561 tcgtagattt gatgttattt tgccgttgct cttgacgtaa tccttccatg attgcacgga
    49621 cagcaaattt tgttccgcaa taaacggcgg aatcaggata aacaacatgt cccgccacag
    49681 aatcagttga aatgatatgc ccagattttt gtttttccat gattggaagt acggcggaaa
    49741 ttccatttaa cacacccatg atgttgatat caagcatatt ttgccattca tcacggtgac
    49801 cttcaactaa tggagcggtt ggcatgattc ctgcattatt gtacaataca tcgattcgac
    49861 cgaacttttc catcgctaaa tcgataactg cttggacttg tgcaaaatct cttacatctg
    49921 ctgtttgaat atatagctca gcttctggta aagattcttt tattgctttt aggcgatctt
    49981 ctcggcgtgc agctataact agtttagccc ctttttctgc taatagcttc gttgttgctt
    50041 caccgattcc gcttgaagcg cccatgatga caatcacttt ttctgataaa tcattcattc
    50101 tattctcctc ctgttttgaa aaatctcttg cttatactca ctatctctta tttcatctta
    50161 ctgttcaaat aatccgttcg acaggtttga cgattttggc agggattcct gcaacgatgg
    50221 tattgtctgg gacatctttc gtaacagttg cattcgctgc aacaatggca ttctcgccaa
    50281 tcgttacgcc tggcagaata gttgctcctg ctccgatcca agcatttttc ttgatgtgga
    50341 ttggctttac tgacaagcct cttcgttctt ttggatcggt tagatggttc accgtgatga
    50401 ggttgacacg aggaccaatc aatactgaat cttctaaaca gatgccacct aagtcaacga
    50461 acatcacttg acgattgata aagacatttt tccctatact aatatgtttg ccgaaatctg
    50521 tataaaaagg cagcagaaca gtcaccgtat catctacttt gtcactaatg atattttcta
    50581 gccgtttgcg gatatctgct ggttcatggt acccagtatt cagcgctgct aaaggctctt
    50641 cgttttcagc ttggatctga tggatcactt caaagagcgg atccgttact cgaatctctt
    50701 tattgacgat ttgtttttcg atgaggcttg tcattttgct ccctcctttt atccttactt
    50761 tataacggac ggcttatcat gtctaatact tataaaacat acttaactat tccttttaac
    50821 tatacttagt ggtatgcttt tttaaaaaca ggagggaaaa aatggatatt cgtatactga
    50881 cgtatttttt agcagtagct agagaaaaaa atatcagccg agcagcaaaa tctctgcata
    50941 tcacgcaacc tactctctca aaacaactga aagatttaga agaagaactt ggtgtgacac
    51001 ttttcactcg gggctcccga gagatacagt tgacggattc tggtcgatat ctttattcaa
    51061 aaggaaaaga aatcttgtcg ttggtagaaa aaacggcgaa caatttaaca aatgacgcaa
    51121 tcatctctgg agaaatctac attggcggag gcgaaactaa agcaatctct ttcatttctg
    51181 atagccttca ttcgttgatc aaggcgcatc ctgatatcca gtttcattta tatagcggga
    51241 atgcagatga tatcatagac aaactggata gcggattact tgattttgga ctcgtaatcg
    51301 aaccgacaaa caaacaaaaa taccagtcat ttcgactgcc agcccaagat cgttggggac
    51361 ttttgataca tcgaaatcat cctttagcaa agcaaaccgt catccatccg caggaactga
    51421 aaactgttcc tttgatcatt tcccggcaaa cgtcagtgga cagtcaacta gctgaatggc
    51481 taggtttcag catagatcag ttatcgatcg tcggaacata taacttattg tacaatgctt
    51541 ctttgatggt cgataacggg acttttggtg cattagctct agatgggatc atcaatactt
    51601 atgggacgga aaaaatattc atcccactcg atccgccatt acaagcttca ctgaatctca
    51661 tttggaaaaa agaccagcct ctttctcctg ctgctcgtaa atttttagat gtcctaacgg
    51721 agaaatacag ttaagtagag tttttttcct tcgctttcag ctttttaaca acgaataaga
    51781 taagcaaaag atccaacgat taaataacat aagtttctta atttatttct tccttttatt
    51841 tctgataatt ccagtaaatt actataatta taaaaaaaga attgacaaaa cataactgtc
    51901 tatataaaat tgattacgtt aacaataatt cgtttcatgt gactagatca tactaggcat
    51961 gaaaaatctc tgaggaacat ggtttcccta ggaggttttt cgtgtctatt tttgtttggc
    52021 cgcaatcaac tggtctagca aacgtgaaaa ccaaatcttc aaaaagggtg cgcgcatgaa
    52081 aatcaaaaag atctttaatc aaaatgcagt attggtagat gacggtggac aggaaaaagt
    52141 agctattgga aaagggattg gatttgacag aaaaagaaat gatttgattt ttgatcgtga
    52201 tgtcgaacgg atatttgtca tggaaccaga aggtcaaatc aagttgcaaa atttacttaa
    52261 tcagattgat gaacgctttt tatttgcagc agaaacgatc attgatcatg cggaaactgt
    52321 tttgatggaa aagttgaatg aacatgtatt gatcgctttg accgaccaca ttgctttttc
    52381 tgcggagaac attaataacg gtatcgtcat ccgcaacaaa cttttaagag aaatcgaagt
    52441 gctttacagt gatgaattca gtattgctca atgggcagta gagtatctga caaaagagct
    52501 tgggattcca tatacttatg acgaagcagg atatatcgca atccatattc acagcggacg
    52561 aagcggtcgg aacaataacc atcgcagtat ccgtgaagtg acgattgtat ctgacatcat
    52621 ccatttagtg gaatcagaat tggaaacaga tatccattca gaaccatttt cactgaatta
    52681 ttcaagactg gtgaatcatt tacgactgct tctacaacga acccatgcgc aacaatatgc
    52741 tgttttagac acggaaatcg tagaaatggt caaacgaaaa tacccagaaa gctataagat
    52801 ttctaaaaaa atccgtgtgt tgctgaccaa agaataccag ctcgcgatca cgaaagaaga
    52861 actgggttac ttggccatcc atatcgagcg tttaagaagc gcaatcgtac aaacaaatgt
    52921 taacaatcat gaggaggaaa aaaattgaaa gcatatctac aacgaatggg acgttccctt
    52981 caacttccag ttgcggtctt acctgctgct gctttactag tagggatcgg aaactggtgg
    53041 gcatcttata gtaacgacat tgtcgctcat tttttacaag ctggcggtaa cgcagtttta
    53101 ggtcaattac ctttgctgtt tgccgttggg ttagcactag ggatgtcagt ggacaaatca
    53161 ggtgctgctg ccttggcagg tttggtcgca tttgaagttc cagtaaatgt tttaaaaact
    53221 gattcagtag ctactttatt gaatatcaaa ccagaatcag ttgatccatc ttttgctcag
    53281 atcagtaacg tatttattgg tattattgcc ggattgattg cggcagctct ttataatcgt
    53341 ttccacgaag caaaattacc gatggcttta tctttcttta gcggcaagcg tctagtgcca
    53401 atcttggctg cctttgtcat gttgctgatc agtgcagtgc tactgctcgt atggcctccg
    53461 gtttactctg ctttagtttc ttttgctaaa ttcattttga gtttaggctg gggtggtgca
    53521 ggtctttatg gcttcttcaa ccgtttactg atccctacag gattgcatca agcaatgaac
    53581 tccgtgttct ggtttgacgt agcaggaatc aatgatattg gtaaattttt ggcttctgaa
    53641 ggaacaaaag gcgtaacagg aatgtatcaa gctggatttt tcccaatcat gatgttcggg
    53701 cttccagcag gagcatatgc tatttacaga aatgcacgtc cagaaaacaa agcaaaaaca
    53761 gcttccttga tgctagcagc agcttttgct tcattcttta ctggtgtgac agaaccactt
    53821 gaattttcat ttatgttcgt tgcttggcca ttatatgtct tgcatgcaat tttcactggg
    53881 atttctttag cagtgagtgc attcttccat tggacagcag gatttgcctt tagtgccgga
    53941 tttgtcgatt acttcctatc tttgaaaaat ccgattgcga atcagccatt gatgttgatc
    54001 gtccaaggat tagtaacagc agtgatttac tactttggct ttaactttgc tatcaaaaaa
    54061 ttccatctga tgactcctgg ccgcgaggaa gcagatcttt cagatgacga tactgcaacg
    54121 actaattcag acaataaata tgcggcacaa gcaagcaaaa tctacgctgc attaggcgga
    54181 gccgacaatg taacttcgat cgataactgt actacacgcc ttcgtttgca agtcaaagat
    54241 acaggcctga tcgatcaaaa taaaatcaaa gccactggcg tacctggtat gaagatcatc
    54301 gacggaaaaa atgcgcaagt catcgtaggt acagaagtac aattcgttgc agatgagatg
    54361 gcaaaactac atggtggtgc tgcagcacga cctgcacaaa caaacaccgt ggttaaaaca
    54421 gaaacatttg ccggtgaaac gatcgaacaa gatatttacg ctatcgcaaa tgggaaactg
    54481 attccaatca ctgaagtttc agatgatgta ttcgcggaaa aaatgatggg tgacggctat
    54541 gctgtattac cagaaaatgg agaaatcttc tctccaatcg ctggtacgat cacaaacatt
    54601 ttcccaacga aacatgctgt cggaatccaa acagatgctg gtatcgaagt cttgcttcat
    54661 atgggcatca acactgttga cttaaaaggc gaaccattca ctttatatgt agaagaaggt
    54721 caaaaagtcg caagaggtca attgattgct ttagttgatc ttgccgctat tcaatctgca
    54781 ggcaaaaaca cggatatggt cgttgtattc acaaatggcg acaaagtcca aagcttagaa
    54841 atcgaacccg cacgtgatgt aaaagcaaat gataaaatcg gctctgtttc caacaaagcc
    54901 tgagccatcg tcttccccag acttttttat cgataaacaa acaggagctg agacaaaaat
    54961 cttgtctcag ctcctttttc cgaataaaaa cggcgttctc ctgccaactc cgcggtaata
    55021 agtccagcta aacgaaacat atgaagagct attttttatt tgactcttct tattacttga
    55081 tgcagaacag ctttttcaca aaatttttct tcatactcaa aactaatcgg ctttttcaca
    55141 acctctttat acattattcc tgatccaata tttcttttaa tgctgcttca agagaagtta
    55201 atggttttcc caatactttt tctaaatcat cagaggaaat agctaactga tcctttttaa
    55261 tatcatgttg gatcgctaaa aacatgtcag ctgcttcttg agggaatccg gcttctttta
    55321 gattttcgac aaacgctgca tcacttgctt caataatttc taactcttta cctgtcgcac
    55381 gctctaatgc cttggctaat tcttcatagt cagttagatt gccggataat tctaagattt
    55441 ctggagagtc actttctact agtacttttg ctgcagcttc tgcatattca cgtttcaatg
    55501 cccaacctgt ttttccattt cctgcagcat aaacgaattg accagttttt aaagcctgcc
    55561 caatgatagg taattcattc tctacatacc aattattccg taaaaatgta tgggaaatac
    55621 ctgaatcctt aatcattttt tcagtgaatt ggtgatctgg cgctaatata ctgatcgagt
    55681 gatcggcatc tgcaaaactt gtataagcga tataagatac cccagctgct tgtgctgcct
    55741 tcactacatt cttatgttcg gcttgacgat ttcctggaac gccagagaca aaaagcaatc
    55801 gatctattcc tttcaacgct tctttcattg agtctaaatc atcataatca ccaatacgta
    55861 tattaaatcc agcttctttc agtgcttctc ctttttcttc actacgtact agtacataga
    55921 tttcattttg tggaaccatc ttttttaaga attccagcgc ataaccacca aaacctccag
    55981 ttgctcctgt tactaaataa tgcatatttg ccactcccta ttctttttat ttgttaagat
    56041 ttacagctgt acatttataa attacacctt tatcataaag agtgtggtaa gataagtcaa
    56101 gcaaaaaaaa tgaaaggatg acaactgatg aagtattctc tgcaatttag tgatgcaatc
    56161 catgtacttg cctatattga aatgtttcaa gatacccgat cactttctag tgagatgatt
    56221 gccagtagta tcgaaacaaa tgcagcaaat gtacgtaaaa tcatgagtca gctcaaaaaa
    56281 tccggtttga tctttacaaa aaccggaaaa ccgcagcctt cccttgccaa aagtccagat
    56341 cagatttctt tactagatgt ctatcgcagc atcgaaggta atacacagct tatccaagtc
    56401 gatccaaaaa caaatccaaa ttgcgtcgtt ggtgcgaata tccaagaagc tttagaagaa
    56461 agctacgctc ggctgcagaa aaaggcagaa gaagaaatgg ctaagatcac acttgatagt
    56521 ctgatacata aaatagccgt tttggaaaaa gaaaaaagac cagaaaacac aggcctcgta
    56581 gagagatttt tataattctc tctactgtgc ctgcattttt ctgatcggaa tcgtctacat
    56641 aaattctttt attctccggg ggacaaaagc cttgtcatgc ccctttttcc tctctaatgc
    56701 tcaggtcaaa gcgctgaagt catgacgtct tgataaacgg tgttcaagta gctgaccctc
    56761 ggcattagtt ctgaaaaatg caaaatatgg aaagctattt cccatttttc ctctctaatg
    56821 ctcaggtcaa agcgctactt tcacgacctc tatattatca tggtttattg actaccggta
    56881 tccagatttc tcccatatat ttcccgttgg taactcccat ttcgactaca gcatttggac
    56941 ccccaacata ggcgtaatct tctacttctc ttaaaacatg cccaaaggcc gtattcgtca
    57001 aatcatttct taattcttca ggcgattctg ctaaagcgct tacaacaaga tacgttcctt
    57061 ttgggaatgg gatgattcgc gtagtgtttg gaacatcctg actcgtttga acaccgacat
    57121 agtacatcat tttcccgtta tagggttcgt tgactgtaaa gaaataatga ttttcagcag
    57181 cttgtttcaa tgcatctatt gttccattac cgataacttt ttgctcaaat gcttgtttct
    57241 ctgcagccaa accgacaaaa tccgtattgc tactcttcaa ttccacacca atacccgtca
    57301 ccgtaaaatc tggtttttct tcgatcctat actttgccat ttctcccacc ctttctatta
    57361 tattaagttc atcttacata aaattaaagg gaaatgacac gattacgctc ttttttctag
    57421 ggatattttt attttttaac tatttttttg ctttcactgc aatccaaatc tcactgtaaa
    57481 cattactgaa atcctctccc cattttgtaa aagagatctc gggagcttcg actaacccat
    57541 aatctgaaga aggaagccat tctgcataga ttttccccca agtatcttgt aaagtttggg
    57601 gaaatggccc tttattagga aaaatggccc aagtgtgttc ttctatagca agcatttcta
    57661 aatcagcgta cggattttct tttgtcgtag caaagccaat catgtgcatc aaactgccct
    57721 tttcttccaa gcggtctcca tcaaatccat aagaaacgtt gattacttga tttggatata
    57781 aatctcctaa cttatgcatc tcttcccttt gagtctccgt gatcgtctgc gctaactttt
    57841 gtatttcttg attgactcct tcaaatttga caggaacttt tttcgccaca cctgccacat
    57901 aaaactgttc tttcttttcg atacgaaatt ccatcgattt tcctcctctg acatcaataa
    57961 aaaatgtcaa gcgtggaaaa gatttttgta tgccgtatct actgatttct gaaggcagat
    58021 atcctgacca atcacgaaaa gctcttgaaa aaccttcgac tgtttcatat ccatatctaa
    58081 acgccgtttc tgtcactctt gatccatcta gaagatcgcg attagccaaa gccaatctac
    58141 ggtttttgat atagtccatt agcgacatcc ctgcaataaa cgtaaagatg cgttttaatt
    58201 gatattcaga gattcctgta ttttcggcta ttattttata ggaaagtcct tctgtcaaat
    58261 gttcttcgat gtaatcgatc aattggttca gatctttaag catatttatc ttcctccttg
    58321 attgcctttt tatcatatta tcaaagaaag tcaattactt gatgaatcag gaaccaattt
    58381 agtcagatgt agaaaatgtt gtatggatct aaagaaaatt ctcaagcagc gtttcgtaac
    58441 ggataattgg tattatctgc ctatctatgt tttaccttta gaaaaataaa ttttggggaa
    58501 gacagattct aagtaaacaa agcggtaaaa atatctttta ttcccttagt taatccgctt
    58561 actacctact gtacaaagca aattgttgtt caaactgctg atcagaacgc tgcttggatt
    58621 tttctttgcg atacatcata cctcttttat caattggaac aattatttat ttctaacaaa
    58681 atcacttcat ttttgtcaga taaataaaaa acaaaaaaag ttccatcttt aaaacacgct
    58741 attcttttac tttttctcac attctctaag aaaagaaaag gagaatgaat ttttgacaat
    58801 tcgagcaaaa gtgattatca aatatgtaaa atcagctact tttttcttaa acgtttcttt
    58861 atactaaata taaataagtg tttcatgaaa ggagaactag atgaataatt ggcttgagta
    58921 ttttcctgaa aacgttttgg aacgcggtta cagctatcat ctgcacggtt ttgtccgtca
    58981 tttgaactat acaagcaagt acttgtccgc aacagtttct ggcacggaag attataaggt
    59041 agtgatcact tgggatgaaa agaccaacat gacttgtgat tgtctatatg ccatagaagg
    59101 aaaaaaatgc aaacacatgg ctgctgtctt attcgcttac gaagaacgtc caataaaaaa
    59161 atccaactac tcactttccg aactctcgtc ccttgtttcc tccgctagtt cctctcttgt
    59221 acgggagctt ttaacagaga ttcttataga acatccgcac tttatcgaac gcttcaaagt
    59281 taagatgcca tttcatgcaa taaattattc agataaatta acaactatca tccataaata
    59341 cgatcatatt atcaagaaaa acaaaaacag gaaaacagcg aagttcatca tggagatgcg
    59401 aaagttcatc caagaagcag ttgaatcgct tattcagcaa aatgcctatc ttcccgcatt
    59461 cgaattgatc aatgaagtga ttgctactct cgaaacattc tattgggagc ctgaggatga
    59521 acgaaccctt cttctgatag aggactgtta ttatttatgg aaagaattat tagcagaagc
    59581 acctcatgca gaaaaaaggc aaatgttctc ttggttcgtc tgccaagtcg atcatactga
    59641 cgcctcttat tcaaaacgct actctataaa aatcctgaaa gaagactttc gggaaaaaga
    59701 attttctaac caaaagaaaa aaatagataa aaaactaaag aaacggtaaa gaaagacgat
    59761 ttcaatgaaa aatagcaagt aaacgataac aaccatccta aatccacaag aaatacagca
    59821 gaccttgctt taaaaacacg aatcatgcaa aggatgaagt aaagaagact taacgcgtac
    59881 ccaatattag tcatttgact attaggtacg cgttttgttt tataagtcat gacaaatttc
    59941 cttcgttgct ggatagattt tccggcattg ttgatagatt ttatgatact tttctacatt
    60001 ttgtaagaga ggttccgctg catctgtata ttggataaaa ttctgacaac aactttcagg
    60061 tgtgtcgaac caccctaacc ctagtgcagc aatcattgtt gcgcctaatg caggtccttc
    60121 ctccacactt aaacaataga ccgttgcatc gaaaatatca gcttggattt gtagccatgc
    60181 aagatttttc gcgcctcctc ccaccgaaac aatacgtgta aatctgcgac ccgctagttc
    60241 tttacgtttc aatcccacca ttgacaaatg ctcattaaaa atttagaata ttcacaagtt
    60301 taacgacaat tatgaaaatt tatgtcgtgc taaaggagtt ttgttattgg atgaaaagtt
    60361 agatataaaa actgcgatca aagacacgtt acctaccgtt ttcggttata tcggtattgg
    60421 acttgcattt ggtatcgttg ggaaagctgc cggatttcat ccactagtcg tcacgttgat
    60481 gtccctgctg gtctatgctg gttctgccca atttatcaca gtcagcatgc ttgctagtca
    60541 cagcccattg ctttccatcg ttttctcgac ctttctagtc aattcccgaa tgattctgat
    60601 gagtatgacg attgctcctt atttcaagaa aaatcggctg cttcaaaatc tgctgattgg
    60661 cacattgctg acagatgaaa gtttcgcact gggaatgaat aaattgaact atactggaca
    60721 aaagttaaac tttcgttgga tgaatacagc taattggatt tcttatttga cgtgggtggg
    60781 ttcttctctt gttggcgcac ttttagggaa ttttattact gatccgaaaa agtttggttt
    60841 ggatttcgcc atcgtcgcta tgtttatcgg gttactttat cttcaagtca tttctgatag
    60901 aaacacctct aaacgattac agttgatcct gattggtctc actttgattc tggtatatgt
    60961 cggactgatt tttatcccaa gcaacttagt gattgtagtt gtaacactga ttgggtgcgg
    61021 attgggggtg tggatcaaac atgccttctt ttgagtttac attgctgact attttaggtt
    61081 gtactttagc aacttggctt tctagggtcc taccatttgt cttactgaaa aaattcgatt
    61141 taccgcaacc gttgcttgag tatttaagtt ttgtcccgat cgtcattatg tctgctttat
    61201 ggttcagcag tctatttacc caaaatatcg gacatctgcc gcagatcaat ctcgaaaatg
    61261 ctttggcttc agttcctaca cttttagctg ctattctttc aaaaagctta ttggtgatcg
    61321 tcttggcagg aattctttcc ctttcgttga ttcgcttatt ataaataaaa aaacaagagc
    61381 ttatggcaaa ttactgtcat gcgctcttgt ttttatcaaa gtcaaataag aaaaaaatcg
    61441 gcgtattctt gcttatccgt tgaacggacc actaacaacg acttttccta acatatgtcc
    61501 tgtttctacg tctttggttg cttgtttcaa gttcttcgca ttcatcccat ctgaatagac
    61561 cttgctcaat gtactgcgga taatcccttc atttgctaaa gcagcaatgt gcgctaaagc
    61621 ttctccttgc gtttcgattt catagttata gtctgtttta gcgaacatat actcccagtc
    61681 gaagctgata gattggtttt tccaaacacc tatatctaac ggctctttga tccctacgat
    61741 cgttcccaca tgtccaaatg gtcggacaag gtgcttgatt tgatcaaagt agcgggtaat
    61801 atcgaataat acagcgacat actctacttg atcgattcct aattcacgaa aacttttact
    61861 taagtcgtga tgataatcga ttggatagtc tacacctgtt tttttcagcc attcaaaatt
    61921 cttcggactt gcagtcgcgt agacttctag gccagcccaa tgcgctagtt gattcaaaat
    61981 cgaaccgacg cccccggatc cgttgatcac taagattttt ttcccgcgat tggcattttc
    62041 ttctggagtt agaccaaatt tttcaaataa caattcataa gcggtcaatg aggtcaacgg
    62101 caaggctgca gcttcttcat ctgaaagatt ttttggtgca agagcgacaa tacgttcgtc
    62161 aactagttgg tattcttgat tgcttcctgc tcttgttgtt gtacctgcgt aaaaaacgcg
    62221 atcgccgaca gaaaattttt gcacttcgtc acctacagca acaactttcc cgactccatc
    62281 aaaacctaag atacgtaaag catttcttat cccgttatct tggcgaagtt tcgtatcgac
    62341 aggattaaca gatactgcgt tgacttttac cagaagatcc cgtgaactgg gtacaggtat
    62401 ctgttctttt tcatcaataa agctttttgg atcatcgatc ggcaaccctt cataaaaacc
    62461 gactaccttc attaattcct tttctgacat acgaattcct ccttagcttc atcaagttgg
    62521 agcatccata cagtgttcat ttcaatatat atagtatgct atttgttcca ccttttttat
    62581 tcatcataat tattctctta cttaaagtaa actatttgca cttttttggt taaaaaagag
    62641 gttgcgacaa gacttttgtc acaacctctt ataaacggtg ttctcctacc aactcttcgg
    62701 tcacaagtca aaatattcat caaacatgag aagctgtttt ttgaaattgc tccttgtgac
    62761 tcagagttaa acggcaggct cacaacctct gatataaacg gtgttcaatc aactgaccct
    62821 cggtattagc tcaaaaaacg acaaaatatg aagagctatt tttgtttggc tgttcttatt
    62881 acttgataca ggacagcttt ttcacgacct ctaattcacg gtgttcaagc aagaaatcat
    62941 tgttcactga aaagcgttgg atgttctttt tcaaaggtct ctaagatctc tcgtacttca
    63001 tcagtagatt tcgtattcat caactgaact cgcaagtcat ttgctcctgg aaatcctttg
    63061 atatagattt taaagaaacg atgtagtcct gtgatggaac gcgggatgat ttctgaataa
    63121 tgatcttgca gatctagttg cagacgaagt agtccgatca gttctttttc tgtatgttcc
    63181 ctcgggtctt tttcaaatgc ataaggattc ttgaaaatcc ctcggccaat catgatgcca
    63241 tcaacgccat atttctcggc caattcttct cctgtctggc gatcggggat atctccattg
    63301 atcgtgatta gtgtttgtgg agcgatttgg tcacgtaaag caatgatttt aggaatgact
    63361 tcccaatgtg catctacttt actcatctct ttacgcgtac gcaaatggac agaaagattg
    63421 gctatatctt gatgaagtag gtgactgatc cattcttcca tctcagacat ttccgtatag
    63481 ccgatacgcg tcttgacgct gacaggcaaa ccgccagctt tagctgcttc aatcaattgt
    63541 gccgctactt ctggacgaag gatcaagcca ctgccttttc cacgttctgc tacattaggg
    63601 actgggcagc ccatgttgat atcgattcct tggaagccca tttctgccat agcaatgctc
    63661 atttctctaa aaaattctgg cttgtctccc caaatatggg caaccatcgg ctgttcatct
    63721 tctgtaaatg ttaatcttcc tcgtacactg tcttttccat ctggatgaca gaaactgtct
    63781 gagtttgtaa actcagtaaa gaagacatcc ggtgctgctg catgctttac gacatggcga
    63841 aatacaacat ctgttacatc ttccattggt gctaaaacaa aaaaaggctt tggcagctct
    63901 ttccaaaaat tagtagtcat ttatttgtta ctctccattc tatcaagcag tgaagtcgca
    63961 atctttctca aaaacattgt cattatacta ggaaacaaaa aaagaagcaa agtgaacagc
    64021 ttaaaagatg tttctgattg agcaaaaaaa agactgtgac aaaaatctta tcacagtcca
    64081 ttttccggaa taaatggtgt tcaaaagctt gcatttgcgg taataagtcc aaccaaacga
    64141 aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcaga acagcttttt
    64201 cacaacctct ccactaaacg gtgttcaatc agctgaccct cggtattcgc tcaaaaaaca
    64261 acaaaatatg aggaactatt ttcccgtttg tttctctaat actcaggtca aaacgctgat
    64321 ttcacaacct cttttctaac ttctcaatcc tcttccttta ttgatcaaat accaagtaac
    64381 ggcgtacaaa acaacgatga ataacacaag tacgatcatc gatgttgcta ctggcacatc
    64441 gatcgttcca aggaatccgt aacggaaacc ggagatcata taaacgatcg gattgacttt
    64501 tgaaacagct tgccaaaagg gcggtaacat agagatggca tagaaaacgc cacctagata
    64561 agttaacggc tgcaagacaa atgtaggcac gatcgataca tcatcataag actgagcaaa
    64621 aatcccgttg agcagtcctg ctaatgaaaa caagattgcc gtcatcaata atgtaatgat
    64681 gaccatgaac catgaataca catgaagtgg tacaaagaac aaggaaatga tcgttacgag
    64741 agagccgacg agaatactgc gacccacacc accaatcaca aagccccaaa taatcacatg
    64801 cgtaggaaca ggtgctacca agatttcttc gatatttttc tgaaattttt gagagaagaa
    64861 ggacgaagaa acattggcat acgaactagt gatagcagac atcataatca aaccaggaac
    64921 gatgaattcc atatatgaaa aaccacccat gtcaccgatc cgcccaccga tcatttttcc
    64981 gaaaataaca aaatacaagg atgtcgtgat cactggcgga accagtgttt ggacccatat
    65041 acgcaaatag cggttcgttt ccttgatagc taaacttttt aatgcagtaa aataaagatt
    65101 aaacattttt ctcctccgta tgattatttt cttctgtgat tttcaagaac agttcttcta
    65161 aacgattcga cttattcctc atagaaagaa ctttgattcc ttgtcttgta agctgatcga
    65221 agattccgtt caccccttga tttcgttcaa cttcgacagc gattgtctgc tcatcttcaa
    65281 atgtacaagc atatccttca atcacaggtt tggtatcata tggcgctaga tcaaagataa
    65341 atgtttcaaa ttgcagtttt gacagaagct ctttcatact tgtattctcg atcagttcac
    65401 ccgattggat gattccgatg ttgcgacaaa gcatttcagc ttcttctaga taatgcgtcg
    65461 tcaaaatgat cgttgtacca ttttcgttca attctcgtag aaactcccac atctcgcgtc
    65521 tcaactcgat gtcaactcct gctgttggtt catccaagat cagcaaacgt ggttcatgca
    65581 tcaatgccct agcaatcatc agtcttcgct tcattccccc ggaaagcata cgtgcacgga
    65641 catttctctt ttcccaaaga ttggactgct tcaaatattt ttcgctgcgt ttcatcgctt
    65701 ctttgcgaga gacaccgtag tagcctgctt gattcacgac gatttgctgg accgtttcaa
    65761 aagggttaaa attgaactct tgtggaacca atccaatctg ttgctttgct cgaactagat
    65821 ccttgtccag atcataaccg aagacattca cttttccaga tgttttattg actaaagaag
    65881 taataatccc gatcgttgta gacttcccag ctccatttgg ccctaatagc gcatagaaat
    65941 ctccttcttt caccgaaaga tcgattcccc gcaatgcttc gaccccactt ccatagatct
    66001 ttttcaaatt atttatttca agcgcatttg ccatttattt cctatctcct tatagacatg
    66061 attcttaatt gtatcatgga atacagcaga tgaaaaaaca acttggcttc cacacaaaaa
    66121 cacacctgct aacaaagagg agatgtgttt tctagttcat gatcatcttc gatagaataa
    66181 ctatctaaac aactctggaa cttttaaaat cataaaagta ccgacagcaa ctactacgat
    66241 gacatataga agatccttat aactgaggtt cttgtctttt ttattcatgt taagctttcc
    66301 tccgtccttt tatcgaaagc ttttttcttt tattttaacc gaaataaata aaatggaaat
    66361 agttattttc aatttctgtt ttcgctgttt gttttacgga gaactttgcc atggaacgtg
    66421 cgtctcgtcg ctgatgctaa gttttttcga taaaagctgc tagaaggaag acagcgtggt
    66481 ttattttgta tcgtcttttg ctaaatcttc caagatcatc tggacagcag actcgttccc
    66541 attgaattct gccacttttt tggcatcaat gtacttcttt tgataatcgt ctttttggac
    66601 ccacgcatca tgcttcgttt gataaagtta atcaaacgga taccactgac gctgttcatc
    66661 gaacaacaaa ctttcttttt gggcctgttc aactatggat ttaggataat tcaaaacttt
    66721 cagcaaggca atcgcatttc gggtccttga aggtccttct tttaatttga aatcaaaact
    66781 gattcctttt ccttctgtta cttgttcttc aaagtggaca ttctcacaat aattctttaa
    66841 aatctctgtc aattcgatat catgtgtggc gacaaatgct aaagatggat actccgcaag
    66901 ccaccgaacg actgaagcgg aagaagcaat ccgttcgatc gtatttgttc cttttaaaat
    66961 ctcatctacg aagcagtagc agcgttcttt ggaagccact tggtccagca aacgtttgat
    67021 cgatttgatt tctgaaacaa aataactgtc gccttcaaat aaatcatctt cgattgccat
    67081 ggaagtcaac acatgtccag gttgcagggt aaactgttct gcaatcgctg tttggatcgt
    67141 ctgtgctaga atacagctga tggctatact tttgacatac gtagattttc ctgatgcatt
    67201 ggaacccgtg actaatgtat tcttctgcca gttgactgga ttaactaccg cttcttttaa
    67261 caatggatga tagatatctt tcgctgtcac accgccctca gtaaactctg gtcggcaagt
    67321 gatcggcata taggtgcgat aattcaaaac ggctgctgca acttccaatt ctcccagcaa
    67381 ttcccacagc ttgatggctt cagttgaatg cttttctaat ttagtgagaa cgaaatgata
    67441 ggagataaat ggcagcatga acatgatgtt gacatattca aaaatcaatt ctgcttcact
    67501 cccgctcttc attcgaaatg aaaaacctaa tttagggatg tctcttaatg gtttagtttg
    67561 ctgtttcagc tctttttgaa gtggtgtcgt catttttgct aactgattgg cggctgaaat
    67621 cgttgaaacc aaataactca tgctattcag ctctgtttct agtgtttgtt tcttcactag
    67681 ataatagacc gtattgaata ctaagccacc gattgctaga tagattccta gtacttgccc
    67741 taaaagaagc aaacctacac ccaccagcgg aagcaagcca agaaaaataa ataaacctac
    67801 gcgtccaatc tttttacttt ttccatccgc tagatattgt tttgcaaaat tatgatcttt
    67861 ttttcctaaa cgagcgaatt gatattggat tctttctctc agttgaggat tttcctgata
    67921 aaaagcaatt agtttttcta actgataatc ttctccaaaa tcaaagctac gtaaacgctg
    67981 atacaacgct tctgaaccta cactagaata ggtactgttg atcccttcaa atacttcgaa
    68041 catatccaag tcataccaag taagatcgtc gatttcacta tcccatgaac ggaatttttt
    68101 ctccgtgagc cacgcatcct tcaaactttc ttctttatca aatctcggtt gataaggaat
    68161 ttttccccaa ttactacgaa ctgtttcctt tactttcaaa cgtgaaaaaa tgtctaaaac
    68221 aactaataaa ataacaactg caataataat taaaacgata aattctcttt ggcccatatc
    68281 cctctccgtt ccttgatttg ttagttttag tatagcactg ttgcgctatt tgaaaagggg
    68341 gtacaaaaat aactgtcttc gttacctaat tcgtatcaat cacaccaaaa taaaaacacc
    68401 ttccaaaaaa gaagatgtct ttaaaacgag atacaatttt ttgtcattca cttcaggtaa
    68461 attagagcca attaaaccct ccgcaacttg acggagatgg agaagatgcc cccgtctaat
    68521 ctagcggtca tctctcctcc cattccttcc accaattttt ttgacaggta aagcccgatt
    68581 ccgctagatt ccgtatttgt gcggctggta ttttcggtat aaaaacgctg gaagactttt
    68641 tcaacagagg tttgcgctcc tggtttgata ggattggcca ctgttaatcg aatgaatgaa
    68701 tcctcttttg aaaatgtcac ctttgcttgt ccatctgcat aacgcaataa attctgtatg
    68761 atattttgca gcactcgcag caacatgagc ggatctgcta atacagttgt atttatcgag
    68821 tgttcagcga acgctacctc gatatttttc tcagaaaatt gttcataaaa agagaaaaat
    68881 acttcttcca cctctttaga aaaagaaagc gtatggagat ccatttcttt ttgcttcgtc
    68941 tctattcgag caagatcata aaactgatcc gtcatactga gtaatcttgc aacagattgt
    69001 ttgatgatcg ttaaatactt ctcccgtttc tccgtatcct ctacctgttc cagtaattgt
    69061 acataaccgt taatagcagt caaaggagtt ctgagatcat gagaaaggcc gacgatcgat
    69121 agtctgatgt tttcttccat ttcttgaaat gcttgtcctt tttgttcatt ttcatccacc
    69181 catgtattca gtgcatctac taggtccatt agattctttt ctcgaaattc gactgaaaga
    69241 cgaccaccat attcttgtcg ttcagaaaga gtggcgattt tcttagttaa tctcttgatt
    69301 tccctttgca ggaagaaaat aaaacttccc ataaagataa caacgagaag caacagccac
    69361 ttaatcaaat ctaaacccca ctccccacac cgtatgaata tacacagcat gtgcctttgc
    69421 tagtttccct cgaagattgg aaatatgcat attcactgtt ttctcactat caaaatatgg
    69481 ttcttgccaa actgtttcat aaagatttgc tcgcgaaaat acttttttcg gatgttctaa
    69541 aaaaagcttc agcagatcga attctcttcc tgtcaaatgg acagccacac cattcacatt
    69601 tacatccctc gactctttgt ccagtaagat ttccttgtaa tagatgcgtt gcgacagatc
    69661 ttttacttgg acagaagtct gatgacgaag ttggatgccg attcgtaaca acagttcttc
    69721 gatatcaaat ggtttcgtca catagtcatc cgctcctgct gccaacaagg aaactttatc
    69781 tctttgatcg ctttttgctg aaagaacgat gatcggaaca ttcgatgtct gtcggatttc
    69841 tgctaacaat gcttcacctg aaagccctgg aagcatgaga tctagaagga tcaaatcgac
    69901 aggatatgtg cttaacaata attttccttc cgtacctgaa taagcatcaa gaagtttgta
    69961 tctctgttca agaacctcct ccagtaaaga atgaacagct tcatcgtctt cgataatcaa
    70021 aatagtttcc agcaggaaca cctacttaat gtcagatttt tggaaaataa acattcctag
    70081 agctgatgat accacgattg ttactagact gaccagaatc attttcggtg cttgcgaata
    70141 agttgcttgt tgcgcaaagt ccgtatacat gagccacgtt tccgtaaacc actccgttag
    70201 aaattccaag cctttgattt ttgcgaaaac accgggaacg aaggccgttc ccatgatata
    70261 aagaaaagaa agaagcaaac tgacactggt acttctagtg atgaatgtca aagccactgg
    70321 aacagataat gtcgctaata tcagcagaag caatacagga aatactttta gaacataatt
    70381 tccatagttt ttgattccct caacttcacc aaatccgaaa gcaagtgtgt aagcaatcat
    70441 ggctagcccc gtaatcaaaa ccacgccaag tgttgtgatc aaaagcgtaa cgagaagttt
    70501 cgacacataa taagtcattc gattccgtcc taaagataac gtattgcgaa tcgtaccgtt
    70561 ttgaaattct tccccaatat aaaagcacgc aaatgctgcg aaagccagaa gaatccatgc
    70621 agccattttt tccaccatta aaatccctgt tgcaccgttg atgactgtat ccgttgcttg
    70681 gtttaattca ttgccgtatt ttgtatgcat ataccattcg ttccaagaat aaaaaagcgg
    70741 aagtacgacc atcagtccta gtgagaccca cattttacgg ctcttccata cttttatttt
    70801 ttcagcagtt aatagattaa acatgtggat tgcctccagt taattgtaag aaataatctt
    70861 ccagtttttg cccaactagg tggataccat ccacaggaac ttttgcctct accaatgctt
    70921 gattgatggc tgctacttgg tcaattcctt caaaaagctg gataacacgg ctagattgga
    70981 caaaataatc cctataacca agagagtcga gaaccgttac tgcttgttcc gtcgccgaag
    71041 tgtcaatttt tataaattga cgagattctt gtgccaattc ttctttcgat aattctttga
    71101 tcaagtgtcc ttcatgcaaa ataccgtagt gagtggcgat ctgtgacaat tcatctagca
    71161 agtggctaga aagcaagacg gtgatgcccc gttcttttac caaacgttgg atgatttctc
    71221 gcatttctgc catccctgct ggatccatcc cattcgtcgg ttcatccaaa atcaaaaatt
    71281 ctggattcgt gatcagtgca tttgcaatag ctaaacgctg gcgcatacct aaagagaaat
    71341 tctttgcttt ctttttccct gtattttcca atcgcatcat tttcagcagg tcttcgattt
    71401 ctctatccga cacaccgccg ttcgctgctt ggatacgtag gttatctctt gcagttaatt
    71461 ctggataaag agcaggggtt tcaatcgatt gacccatacg tcgtcttgct gcctgcaaat
    71521 ccgtcgttcc aaataattcg atcgaacctt catcaatcga gatcaaaccc ataatagttc
    71581 tcataaaggt agacttaccc gctccattct ccccaatcaa accatagatc attccacgtt
    71641 tgatttcgat cgatacttta tctaatgctt tggcagcacc gtattttttg gttataccag
    71701 ttgctttcaa gaccgtttca tttccaaaat ccatacttat ccttctttct tattgataag
    71761 tgaagtatag ttgacgggta taaagaaagc agccagaaat gtataaagaa agtgtaaatt
    71821 ttccaattga atttcaagaa aaatcagact gtctcgcaga cgtggttgag ttgtctgcca
    71881 atcaaagagc gcaccaaaca gtgctaatcc aagtggactg gccaccatgg taatcgttgt
    71941 tatcgttgac atcagacgtc cttgtatatg cgttggaact tctcgctgaa gccagaccat
    72001 ataaaaagca aaagaaaaca ttaatcctta aaaacggact tatgaaaaat aatacatgcc
    72061 gtttgggatt tacaattagc taccaaataa gaggttatga caaaagactt ttcacaatcc
    72121 cttcttccga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatttat
    72181 caaacatgag aagcaatttt tgtaaattcc ttcttatttc ttgaagctga ccacttctgt
    72241 cacaacctca gaagcggata aataatattg tttgatgata ctttatcttt ttcccgtttg
    72301 tttatagatc gaatgatatt atagaccaag aatcctacct attaacatag ctaaaaacac
    72361 aaaaaaggca ctttccaaat tgtggaaagt gccagaaaag ttaggattaa ggtaactgcg
    72421 ctcagcattg cttcgcccag tacgattctc gtaactcgtg aactcgaacg ataatcgcaa
    72481 ttaacgtttt gagaactgtg aagctttacg ggctttctta agacctggtt ttttcttcgt
    72541 atcattgaat aagtttgaaa aaccttgatt tataaaggtt ttcccgtaaa tatattttat
    72601 tatatttgat taaatttgaa atgtttgtgt tctaaaatgt gttctaattt aaaggcagta
    72661 tatttcgtat tttagattaa atttttaggc tcattcattc tcaaatccat tctattcagt
    72721 caataaaaat tttaaaaaga ctttataaac aaagatgttc aagcgagcat cttatttttt
    72781 tgtgcttaat ttgaaaggag aacatttcat gtataaaaaa caaaaaaaga aattatgtgt
    72841 attaactatt ttgagtttgg ctgttcaagc aattgttgga gcaattgctc ctacgattac
    72901 ttttgctgat gaaattaccc atccacaaac ggtaaccgta gaactagatt tagcacatca
    72961 atatgctgtt gaaggaactt ttagtgatgg tcgtccaatg tcagaagtca ctgttccgca
    73021 ttatgcagtg tacaacggct ttaaacaaga tattttctgt attgaaccag gagtcccgat
    73081 ttacaatgag ttcactcctg gctatgagaa aaacccgtta ccagatatgt ccgataaagc
    73141 caaattagtt tccgttttat ggaaaaaagt aggtacagat gcagatactc atattgtggc
    73201 acagaagatg atctggcaag aagttaatgg gtataccctt cactcaataa aacgatcaga
    73261 tggtagtgcc gtaaatatcg cagccattga ggcaaaaata aatcaagcca ttgacgatta
    73321 tcagaagaaa ccaaccttcc ataatagtac ggctaaaaca gtattagggc aatcgactac
    73381 tgtgacggat acgaataatc tgaatttgtc agagttcgat gaggttgtgg aaaatgccgc
    73441 aaacattgat tatcgtgtga atggtaatca gttgataatc accccaaatg ccaattcaaa
    73501 agaaagtggt gtgctgacgc ttaagaaatc tgctggtacc ggaacacctg tcgcctataa
    73561 aaaggctggc caacagactt taatggccgg ggcgattgat aaacctaata cctacacaat
    73621 caaaattgat gtggaaacgg agggttcttt aaagatcaaa aaagtcgata aagaatcggg
    73681 tgatgttgta ccaggaacgg ttttccattt agactttgga aaaactttac ctgcaaaaga
    73741 cgtgactact gacaaagaag gcattgctac attggatgag attcctcatg gtacaaaagt
    73801 aaccattacc gaaaaatcag taccggcgcc ttatacgatt gataccaccc ccatgactac
    73861 caccattaaa gcaggcgaaa ccatttacgt gacttcaaaa aacgcacggg aaaaagggca
    73921 aatcattctg gataaaagcg gagttgaaac agggagtgat ctttggaatg acaattattc
    73981 cttagctggt aatacgtttg ccattcgcaa agacagtcca accggtgaaa tcgttcaaga
    74041 aatgaccacg gatgaaaacg gtcatgcgga aacaccaaaa gagattgcta atgcgttgga
    74101 attgggaacc tattatgtga ccgaaactaa agctagtcat gggttcgtga atacgttcaa
    74161 acctgtaaaa gtcgaattga agtatgccaa tcaaaccgta gcccttgtta ccagtaacgt
    74221 aaaagggcaa aatcaagaag tcactggcga aaccactttg acgaaagaag acaaagatac
    74281 tggggataaa gcacaaggta aggcagtctt tgagggtgct gaatacacct tgtttactgc
    74341 taaggatgga aaagctgtta aatggagtga ggcttttaaa ccagagttag cgaagggaac
    74401 gaaaggttct gatgaaacgg tcactttgac tttagacgaa aagaaccaag ttgccgttaa
    74461 acatttagct atcaacgagt attattggca agaaacaaaa gcgcccgaag gatatagcct
    74521 tgatgaaaca aagtatcctg tatccatcaa aaaggtggat gatagcgaaa aaaatgccgt
    74581 gattacccga aatgttacgg caaaagaaca agtgattcgc tttggctttg atttctttaa
    74641 atttgctgga tcagctgcgg gcactaccga aactggcttc aatgatctga cctttaaagt
    74701 gtcgccattg gaagggacca atgaaatcac aggtgccgaa gatgaagcga ccacagctta
    74761 taacgagcaa ttagggtttg atggttatgg caagtttgaa aatcttcctt atggggatta
    74821 tttacttgaa gaagtagaag ccccagaagg ttttcaaaag attacgccat tagaaatccg
    74881 ttctactttt aaggaaaaca aagaagactt tgtgaagagt gagtatgtct ttaccattac
    74941 cgaacaagac caaaaacaac caattaaaac ggtgaccgtt ccttacgaga agctgacgaa
    75001 caaagcattt tctgttagtt tgaaccgttt aatgctctat gatttgcctg aggaagaaga
    75061 tagtttaact tctcttgcga cttggaaaga cggaaacaaa gaattaactt ctctggattc
    75121 taccgagcta gttgataaat tgagttacaa tctacatgaa atcaaagaag actggtatgt
    75181 ggtagcgcaa gccattgatg tggatgcgac aaaagctgcc caagaaaaag atgaaaaagc
    75241 caaaccagtg gtgattgcag aaacaagcgc aacgttggcg aacaaagaga aaacaggaac
    75301 ttggaaaatt cagcataaat taaccgccga acaagtttta aataaaacta ttgtcttatt
    75361 caactatgtg tatgaaaata aggaagcttt tgaagcaggc gataagccag tagcgaagga
    75421 tgttagcttg aataaccaag cccaaactgt cagttgtaca gtggaacacc atgtttctat
    75481 ccaaacaaag gcccacctag aaaacggttc tcagaccttt actcatggtg atgtggtgga
    75541 tatgtttgat gatgtgtcga ttactcatga tgtactggat ggctcaaaag aagctttcga
    75601 aacaattctg tatgctttat taccggatgg tacaaacaaa gaaatttgga aatctggcaa
    75661 gattgattat gaagtaaatg ataaagaatt caccaaaact gtgcttgcga aaaaagtaga
    75721 taccggaaaa tatccagaag gaaccaagtt tacttttgcg gaaatcaatt acgataaaga
    75781 tgggaatatc aatggaaaac acaatgaaga tttgaaagaa aaatctcaaa ccttaacacc
    75841 aaaagaagtt ccaaccatac taagtacgcc gaaacaaccg gaaacaccaa ctgttccaag
    75901 tggctctcaa gaatctagtc ccacagtgaa gaccttccct caaaccgggg agaaaaattc
    75961 caatgtttta ctgttcattg gttttacttt gatttttgcg acggcgggct attatttctg
    76021 gaatcgccgg aactaaggtg atgagatgat acaagtaaat cctaagaacc aacgtgcgcg
    76081 accgcctcct aaaaaagtag tcgcgctatt tttgtatcca aaactaacaa tgaaaagagg
    76141 aaaagaagat ggaattgaaa tttgtcgtgc cagatatggc tgaaaccttt ggaaagttaa
    76201 gttatgccgg tgaaggagaa attataacag aaggatatgg acggaatact acggtgattg
    76261 gtcgcagtta tcatttgtat tccagtaagc aacgggccga tgatattgag gtggtagtag
    76321 ctgcggaagc cggtgaaaag gattttgatc aagaccaacc gctaaaagcc gtgaatcccc
    76381 atttggttgc caaaggctat gagattgaga atcgtgggtt tactgattac gtgttgtatg
    76441 ttgatgattt agtgaaagca taggaggaaa agaagatgag attaacagaa ggtattgtcg
    76501 tagattcagg tttaacattt gggaagttgc gtttttccgc attacgccga gaagtacgga
    76561 agcaaaatga ggatggaaca attggtaatg aggtaaaaga acgaacctat aatttaaaat
    76621 cctctgcaca agggcgaatg attcaagtta gtatcccagc taatgtaccc ttacgcgagt
    76681 ttgcttatga tgcagaggta gagttggtca atccaatcgt ggataccgtt gccaattatg
    76741 tttttcgaga aggaactacc gttaattggt tcattaaggc ggatgattta gtcttgaagc
    76801 ggcagccgaa tcaaggaaat cctacgaacc aaaatgaaag aaagaaatag gtgaagatta
    76861 tggaaattaa tgttgaacat attttagatt gtctcgatca atatggaaaa ggcgagctga
    76921 ctgaggaaca actcgttaaa gcattaacct ctgatgaaaa aatgtttctg attatacatc
    76981 aaggtttact tggagaaaat agcgacagtg aagaagactc tgatgtacta gactggttag
    77041 gagaagaaga gtctttcttt atagttgtcg aagtaaatga aaacttatgt cggcaggcag
    77101 aatccatttt ggaagaaatt ggtgtggaga tgccggatgc tattgaaggc tttttaaatc
    77161 aattggttga aacgaaacaa ctcccctcct cgtagcagta aatgactagc ataacttttt
    77221 gaaattgcct aatgaaatcc tattgggctt tttcgattgg aggtggaaac catcaaaatg
    77281 tacaaaggac atcgcatacg cgcaggagat cagcacttgg tttatcactt tgttcttggc
    77341 tggctactcg ctctgttcat cggttggatg agcgtctttt attttcaaga gtttagacaa
    77401 tttgatattt ctaagctatc actttctact attgaaatcg tttggtctat aaaagattta
    77461 gtatgtctgc ttggaagtct cgctttttca ggagctatga tattgcttta tattcacttt
    77521 tttcttgatc attggcgaag cttgtggcat cgacaaaagc tagctcggat gattctagag
    77581 aaccattggt atgaagtgaa acaaactcaa agtgaaggct ttttcaaaga tttgaatagt
    77641 agccgaacca gagagactat cagttacttc cccaaaatct attatcgaat gaaagatgga
    77701 ttactgtcta ttcgcgttca aatttcactt gggaaatacc aagatcagct tttaaaattg
    77761 gaaaagaagt tagaaagcgg actgtattgt gagttagtag aaaaggaact caaagactcc
    77821 tatgtggagt atactttact gtatgatatg atcgccaatc gaattgggat agacgaagta
    77881 gtggcagaaa atgggactct gcgattgatg aaaaatcaag tatgggctta tgattcttta
    77941 ccccatatgt taatcgctgg tggcacgggt ggagggaaga cctatttcct cctcaccatt
    78001 atcgaagcac tattgaagtc agatgcagaa ttgtttatcc tcgatcccaa aaatgcagat
    78061 ttagctgatt taggtacggt gatgcctcat gtttattcac aaaaggaaga aatttctgct
    78121 tgtgtggaag atttctatga acgtatgatg gctcgtagca aggcaatgaa agaaatgtct
    78181 aactacaaaa caggagagaa ttatgcctat cttggacttc cacccaactt tttaatcttt
    78241 gatgaatacg tggcttatat ggagatgtta acgacgaaag aaagtgcggt gattttgaat
    78301 aagctcaaac aaatcgtgat gttgggtcgt caatctggtt tctttctgat tttggcttgc
    78361 caaaggccgg atgcgaaata tttaggagac ggaatccgcg atca
//
LOCUS       DACEQH010000004        78261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.004, whole genome shotgun sequence.
ACCESSION   DACEQH010000004 DACEQH010000000
VERSION     DACEQH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.004"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(94..522)
                     /locus_tag="IW833_000257"
     CDS             complement(94..522)
                     /locus_tag="IW833_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306404.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(550..1188)
                     /locus_tag="IW833_000258"
     CDS             complement(550..1188)
                     /locus_tag="IW833_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1306405.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1192..3228)
                     /locus_tag="IW833_000259"
     CDS             complement(1192..3228)
                     /locus_tag="IW833_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ1306406.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3354..4019
                     /locus_tag="IW833_000260"
     CDS             3354..4019
                     /locus_tag="IW833_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1306407.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(5954..9103)
                     /locus_tag="IW833_000261"
     CDS             complement(5954..9103)
                     /locus_tag="IW833_000261"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAQ1306408.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(9116..10315)
                     /locus_tag="IW833_000262"
     CDS             complement(9116..10315)
                     /locus_tag="IW833_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ1306409.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(10305..11900)
                     /locus_tag="IW833_000263"
     CDS             complement(10305..11900)
                     /locus_tag="IW833_000263"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1306410.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(12484..13191)
                     /locus_tag="IW833_000264"
     CDS             complement(12484..13191)
                     /locus_tag="IW833_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306411.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(13256..14092)
                     /locus_tag="IW833_000265"
     CDS             complement(13256..14092)
                     /locus_tag="IW833_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1306412.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(14110..15519)
                     /locus_tag="IW833_000266"
     CDS             complement(14110..15519)
                     /locus_tag="IW833_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1306413.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(15533..17356)
                     /locus_tag="IW833_000267"
     CDS             complement(15533..17356)
                     /locus_tag="IW833_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1306414.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(18199..18525)
                     /locus_tag="IW833_000268"
     CDS             complement(18199..18525)
                     /locus_tag="IW833_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306415.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(18571..18933)
                     /locus_tag="IW833_000269"
     CDS             complement(18571..18933)
                     /locus_tag="IW833_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ1306416.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(18970..19185)
                     /locus_tag="IW833_000270"
     CDS             complement(18970..19185)
                     /locus_tag="IW833_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1306417.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            19312..19647
                     /locus_tag="IW833_000271"
     CDS             19312..19647
                     /locus_tag="IW833_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1306418.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(19723..21720)
                     /locus_tag="IW833_000272"
     CDS             complement(19723..21720)
                     /locus_tag="IW833_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306419.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(21818..22093)
                     /locus_tag="IW833_000273"
     CDS             complement(21818..22093)
                     /locus_tag="IW833_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1306420.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            22325..23059
                     /locus_tag="IW833_000274"
     CDS             22325..23059
                     /locus_tag="IW833_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1306421.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            23098..24240
                     /locus_tag="IW833_000275"
     CDS             23098..24240
                     /locus_tag="IW833_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1306422.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(24335..25726)
                     /gene="sufB"
                     /locus_tag="IW833_000276"
     CDS             complement(24335..25726)
                     /gene="sufB"
                     /locus_tag="IW833_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ1306423.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(25731..26201)
                     /locus_tag="IW833_000277"
     CDS             complement(25731..26201)
                     /locus_tag="IW833_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ1306424.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(26188..27423)
                     /locus_tag="IW833_000278"
     CDS             complement(26188..27423)
                     /locus_tag="IW833_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1306425.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(27420..28706)
                     /gene="sufD"
                     /locus_tag="IW833_000279"
     CDS             complement(27420..28706)
                     /gene="sufD"
                     /locus_tag="IW833_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ1306426.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(28719..29492)
                     /gene="sufC"
                     /locus_tag="IW833_000280"
     CDS             complement(28719..29492)
                     /gene="sufC"
                     /locus_tag="IW833_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ1306427.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(29689..30537)
                     /locus_tag="IW833_000281"
     CDS             complement(29689..30537)
                     /locus_tag="IW833_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1306428.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(30561..31247)
                     /locus_tag="IW833_000282"
     CDS             complement(30561..31247)
                     /locus_tag="IW833_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1306429.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(31240..32274)
                     /locus_tag="IW833_000283"
     CDS             complement(31240..32274)
                     /locus_tag="IW833_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1306430.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(32669..33007)
                     /locus_tag="IW833_000284"
     CDS             complement(32669..33007)
                     /locus_tag="IW833_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ1306431.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(33009..33365)
                     /locus_tag="IW833_000285"
     CDS             complement(33009..33365)
                     /locus_tag="IW833_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ1306432.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(33371..34558)
                     /locus_tag="IW833_000286"
     CDS             complement(33371..34558)
                     /locus_tag="IW833_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1306433.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPVRS
                     SRR"
     gene            complement(34749..35594)
                     /locus_tag="IW833_000287"
     CDS             complement(34749..35594)
                     /locus_tag="IW833_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1306434.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            35828..36793
                     /locus_tag="IW833_000288"
     CDS             35828..36793
                     /locus_tag="IW833_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ1306435.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            37170..38213
                     /locus_tag="IW833_000289"
     CDS             37170..38213
                     /locus_tag="IW833_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ1306436.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(38275..38739)
                     /locus_tag="IW833_000290"
     CDS             complement(38275..38739)
                     /locus_tag="IW833_000290"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ1306437.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            38985..39932
                     /locus_tag="IW833_000291"
     CDS             38985..39932
                     /locus_tag="IW833_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1306438.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(40143..42215)
                     /locus_tag="IW833_000292"
     CDS             complement(40143..42215)
                     /locus_tag="IW833_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1306439.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(42148..42525)
                     /locus_tag="IW833_000293"
     CDS             complement(42148..42525)
                     /locus_tag="IW833_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ1306440.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(42654..43214)
                     /locus_tag="IW833_000294"
     CDS             complement(42654..43214)
                     /locus_tag="IW833_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1306441.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            43364..44656
                     /locus_tag="IW833_000295"
     CDS             43364..44656
                     /locus_tag="IW833_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1306442.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(44716..45834)
                     /locus_tag="IW833_000296"
     CDS             complement(44716..45834)
                     /locus_tag="IW833_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ1306443.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(45848..47269)
                     /gene="arcD"
                     /locus_tag="IW833_000297"
     CDS             complement(45848..47269)
                     /gene="arcD"
                     /locus_tag="IW833_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ1306444.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(47515..48636)
                     /gene="mnmA"
                     /locus_tag="IW833_000298"
     CDS             complement(47515..48636)
                     /gene="mnmA"
                     /locus_tag="IW833_000298"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ1306445.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNDKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            49040..49639
                     /locus_tag="IW833_000299"
     CDS             49040..49639
                     /locus_tag="IW833_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ1306446.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(49777..51063)
                     /gene="efmA"
                     /locus_tag="IW833_000300"
     CDS             complement(49777..51063)
                     /gene="efmA"
                     /locus_tag="IW833_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ1306447.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(51334..51678)
                     /locus_tag="IW833_000301"
     CDS             complement(51334..51678)
                     /locus_tag="IW833_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ1306448.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(51815..52951)
                     /locus_tag="IW833_000302"
     CDS             complement(51815..52951)
                     /locus_tag="IW833_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1306449.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(53140..54114)
                     /locus_tag="IW833_000303"
     CDS             complement(53140..54114)
                     /locus_tag="IW833_000303"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1306450.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(54194..55069)
                     /locus_tag="IW833_000304"
     CDS             complement(54194..55069)
                     /locus_tag="IW833_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ1306451.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSEKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            55199..57106
                     /locus_tag="IW833_000305"
     CDS             55199..57106
                     /locus_tag="IW833_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ1306452.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(57647..60925)
                     /locus_tag="IW833_000306"
     CDS             complement(57647..60925)
                     /locus_tag="IW833_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ1306453.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(61196..61741)
                     /locus_tag="IW833_000307"
     CDS             complement(61196..61741)
                     /locus_tag="IW833_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1306454.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(61753..62445)
                     /locus_tag="IW833_000308"
     CDS             complement(61753..62445)
                     /locus_tag="IW833_000308"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ1306455.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(62464..62748)
                     /locus_tag="IW833_000309"
     CDS             complement(62464..62748)
                     /locus_tag="IW833_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306456.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(62761..63324)
                     /locus_tag="IW833_000310"
     CDS             complement(62761..63324)
                     /locus_tag="IW833_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1306457.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            63552..64214
                     /locus_tag="IW833_000311"
     CDS             63552..64214
                     /locus_tag="IW833_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ1306458.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            64338..65534
                     /locus_tag="IW833_000312"
     CDS             64338..65534
                     /locus_tag="IW833_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ1306459.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(65635..67011)
                     /locus_tag="IW833_000313"
     CDS             complement(65635..67011)
                     /locus_tag="IW833_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1306460.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(67048..67914)
                     /locus_tag="IW833_000314"
     CDS             complement(67048..67914)
                     /locus_tag="IW833_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ1306461.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(68003..69028)
                     /locus_tag="IW833_000315"
     CDS             complement(68003..69028)
                     /locus_tag="IW833_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1306462.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(69046..69720)
                     /locus_tag="IW833_000316"
     CDS             complement(69046..69720)
                     /locus_tag="IW833_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1306463.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(69904..70797)
                     /locus_tag="IW833_000317"
     CDS             complement(69904..70797)
                     /locus_tag="IW833_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1306464.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(70871..72568)
                     /locus_tag="IW833_000318"
     CDS             complement(70871..72568)
                     /locus_tag="IW833_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1306465.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(72561..73874)
                     /locus_tag="IW833_000319"
     CDS             complement(72561..73874)
                     /locus_tag="IW833_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1306466.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(73874..77392)
                     /locus_tag="IW833_000320"
     CDS             complement(73874..77392)
                     /locus_tag="IW833_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1306467.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(77685..78143)
                     /locus_tag="IW833_000321"
     CDS             complement(77685..78143)
                     /locus_tag="IW833_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1306468.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT        22602 a        16504 c        13512 g        25643 t
ORIGIN      
        1 acaactaaaa atgaatatat taaaactacc aaaaataact tttaacgctg tttcattata
       61 tgttttttct attttatata aacttttgga tacttaatac caaccggttg gattgtacca
      121 acatgagaat gtgtcagttc tatttcctgt cgcacttgca taggcccata ctccttttgt
      181 tgcatttgct gatcgaatag agtttccaac tttcgctttt gcaccgtgac gattatagac
      241 acgactatag tggttagaat gacctgtttt tctcgtacaa gttgtatcac tataataaac
      301 ccatcttcca gagctatgat taattgctgt tctagaaatt tcccggtcta ctggtaaatt
      361 tgacttaata atattacctt tagcatcaaa atgaatctct cctggcttta aagatttgta
      421 atagatttca ccgtgaactg aatttgcttc cacttcagat gacatagcgt ttcctccaac
      481 aaaacctatc cctagtgcac aaataataac tgcttttttc ataaaacgac atcctttcct
      541 ttttgatttt taaagcatga tcttcttgtt tcctattttg gtcagatatg gatcatgcgt
      601 gactacgata actaacgtac cttgggaagc ttgttgcgat aagatttcaa ctactttatc
      661 cttatttctt ttatccaatg acccagtcgg ttcatcggct aaaatgattt tgctattctt
      721 caataagact ctcgcacagg cgattctttg cttctcacca ccagataatg tgtagatagg
      781 cgtacttagc ttcttgttta tatttacttt tttcaatgcc tcatactttg ctgttcgctt
      841 atctgaaaca tttaaattcg aaaacttcaa tccaatatct aaattaaaat cgattgtttc
      901 atattcaatc aacccaaaat cttgaaaaag aaaagatatg gtatttcttt ttatcaagcg
      961 cttctctctc ccagttaatt gtttcgtctc ttgatcgaac aaatagaatt gtcctgaact
     1021 gaactgatca ttcaatccta atatattcaa taacgtactt ttacctgctc cactttctcc
     1081 aacaacagtc acaagatccc cagcatccgc agtgaaacga tccaaatgaa gaatcgtttt
     1141 gtttcgaatg atttttttcg caccttttat cactatcaac ggctccaatt gttaacctcc
     1201 attcatcagc aataactgac ttttcttttc tgtagacaaa ataaaaacaa ctgaaagaat
     1261 aaaatcaatg aagctaaaga aaaaaatatt aaaaagatat ttaggctctc tttctataag
     1321 aaaaaggaag attacacttg tcgttcccca tttgatgagg ttcatcatga agaaatcttt
     1381 atacgtttca aagagcgaat aaccacttga acgaaagata gcgattttcc gacaattgta
     1441 gacaaaatat atgcataaca tgacatagct caacaagaag aaagaaaaga aagtgagtaa
     1501 aaatatctgt atcaagtaat gaaatgatct gcttaacact ttgacagcta tctcttcagt
     1561 ctgagtatac ggaaatgata actgcaagtt atcacgtaac tgactttctt caatcagctc
     1621 aaataacctt tctttcgaca aagagcctat cttgatcttt aacggtggat agatttctcc
     1681 gctgagaata tttctttcca aatacatact gtttttcaat gtcagtattt caacaaccgg
     1741 ataatgttcg atccagggga tagaaggaat aaatgtataa atgggttgat tgttttttat
     1801 aaatagaaat ttcggttctt tttcactgat ttcttgataa tattctgtta tttgcgccag
     1861 gtcatttttt aaatctgtat gtctatatcg ttcagggatc aatataacag ggtgcgtttc
     1921 tttttcggtt atttggatca accttccaga gacatcaatc aaaggaaatt tcttaagata
     1981 attcagattt atttttatcc ctctgccaaa ctgttcattt tcttcattca agtaatctga
     2041 tacattgact aatatacttc cgtttttatt caaagtttca tataattcct gatctgctgc
     2101 tgatgcaaaa cgattggaat aaataaaatc cacgtggttt ttcccgatgg ttactggata
     2161 aaatacatga tagtcctcac cagcgatttc ttttggtggt tcatgggaaa cagctaactg
     2221 tgaagaagaa aaaacgatag ttgctaattc gacattggca acggtcaaaa gaagagaaaa
     2281 caacaatcca aatgtaaaaa gaatgtattg gatatagttg aacgcttctc ttaacgaagc
     2341 gattttttca actaataaaa gagaaatcaa agagatacca tatattccgc tgaacaagaa
     2401 aacgtacatc tggaacaaat aaagaaattg ctccttataa tacataaaaa taaaaccacc
     2461 aatgattcct gcaattatga atgtaataat gattttcttt ttccccaata aacaccacaa
     2521 tacttgatgt aatgaaaagt cattattcaa caataaactg atttctttat tcatcaataa
     2581 gaaaaaggat gataacaaaa ggaataaaag aatcaatcca atagttaaat acactcgata
     2641 attatatacg tctgtatttg aaataaccga atacgaatag ctattttcaa agtcttcaaa
     2701 aatattagtg gcataatcag tgtgaaatgc ttgattgaat cgatcagtga atagtgaaat
     2761 gacccgttca taggcttctt tttctctcgt catcaagaaa aattcacctt caaaatcttt
     2821 tattttgatc gattcttcaa tggacgttac gcgaaaggag gtattagaat agggtacctt
     2881 ctcgtgagaa gatttgacat ccccttgata atacaatgtc atttcaagct ttggctgtat
     2941 cttgaagttc caagatgtat ctacttcatt ggattcattt actgtttttt tcatataaga
     3001 aagctcagtt tctgatgaga cattttcaaa taccatgatt agctgctcat attcttcttg
     3061 cccaataaaa tgttgtggca atcgaatcgg agtataagta tctgtttgat ggaaaaattc
     3121 atttgtccga tagagtacct gtttatttat actaaccaag gcaaaaccac tcatgatacc
     3181 ggtcactatg attgtgagta aaactgtgaa cttttttttg attatcacca ctctccctta
     3241 cttaaatgat tctttgataa aatcataccg tttttcatca aaaaaacgta tctatcaagt
     3301 gtataggtca tgtagaataa atctatatac tgtagaatcg aaaggagaag aagatgaaca
     3361 ccggagaaaa aatacgaact taccgcagaa attgtaagct tacgcaaaaa gaattaggcg
     3421 aaaaaattgg tgtttccgat aaaacgattt ctagttggga aaattctcgt acaatgccag
     3481 actttgaaat gctgtcactt ttacatcata ctttaggtct gccgattgat tttccttcta
     3541 ttgaatcagc taccgaatca atagaaccaa cattgccaga tgaacctctt gttaacaaga
     3601 aatcttattc atcaaaacaa cccttctcac tcaaaaagac actatctttc tttctgctcg
     3661 tcttttgttt ggtctatctt tttcttatca attatcaata caaaaatgaa acgatcgatc
     3721 gattcaatcc ttttttgaaa ccacaaatcg gctataccac actccctgta ggatacgtgg
     3781 acaattccat cgaacgttgg gtatgtaatg atatctttgg ccatggtaca tggctgtctt
     3841 ttcacgctgg agaggtagaa aaagagaaaa accatgctgt aatcaaacac aaaggaaaac
     3901 acgtcatgaa cgttcgcctg ctcacttggg aagccattcc ctcagattac caagaaatcc
     3961 tcagccaaga ctatgacgac gccttaaaaa gaagccctca ggagccagat aggcattaag
     4021 taggagatga acgtagagat agaaaaaatc aatgcacatg atcttatcca aaaaacaggg
     4081 gtacaaatta attgaaaaag ccttgagatg aaagttccta aataaaaaag ttcagaatga
     4141 taggtatttc tggcttatag tataatattt acgccacagc ctcccaatga ttctttttgg
     4201 aatcataatc aaggatggac attcacaaaa atattttgct cattcgatcc gaatagatta
     4261 acgaccaatc tagtaagtaa tatagattgg tacgtagtca agctgcacat aatttagatc
     4321 aattttagtt cttttttata tttctatgat ctgctcattt acttttatat gatggcttac
     4381 agtaacataa ataaatatga taatcgctgg tagtttatcc aagagtactc aataaaatac
     4441 aaaatggatt gttcaagcgc ctatcataat gataaaatga aacaaataaa cgatacaagg
     4501 aggaagagac tttgagtaat ggcagaacaa ttttttcatt gtaaaaattg ttcagttgtt
     4561 aatctatggg atttcggtag catgggcatt tttttataga ggaatcacac caacctggcc
     4621 gttttatctc ttgttagtgg gaatattagt aggaaatttt gcccctcttc actatcgtac
     4681 gaactttttt ccaactgatt ctggcctttt tcttaaaaca aagacgcgaa aattagaaaa
     4741 cattattgaa atgacaatta cctgtataat cattttctta gttatattcc taaacgtctt
     4801 ctaataagaa tctcttaaaa ttagcttgtt acagttagtt acctagttta tataacacag
     4861 ttatcttatc ttccttatca acatcttttg ccaactcttt gtcttaaatt cgaaacatat
     4921 aagcagagct acgaacacca agactgtttc ggctctgttt tttgcatcaa attcactctt
     4981 atatagctga taatctcggc tctttaaaag aaattatttt cgcttcattt tattataaaa
     5041 aggctaggaa ccttgatgtt atcaggttcc tagcctttat ttaagtatta ccccacagac
     5101 ccttccatat agatttttaa attacacact agtttaaact aaaacaaaca aggaaaaact
     5161 taccatttaa tcaaaaaatt agctcatatg aatatattag aatgtaaatt agactggtca
     5221 aaatctggtc aaaaaagagg acgaatacag gtagtcctct tttttgttat tttgatattc
     5281 taaatcattt tttattggat tattcccttg ttcgatatag acccaaattg atctatcaat
     5341 aattatcaaa ctgagtcata tagagacaaa aatttcaaaa atgacctgtt aaatggtcat
     5401 cgaatatcat ttaaaatcaa tacgacggag tcagacacgg agtcaaaaca agcccgctga
     5461 gagtaaaaaa tatacagtcc cataggtaaa gtgccacagg actgtatatt tttttatttg
     5521 agattacctg aaatgattcc atttaatttt tatctcattt tttcctacta gttagtatca
     5581 aatactctcc tattttctca ataaactatt ttgggttaca tggtatctga aacagctaaa
     5641 accttgtcat tatagcaaaa aatcacttta tctccgtttc atttcagata catttcactt
     5701 aagcggattc caaaaaaaca aaagaaaaag gaacccctag ttagcctatg ctttttattg
     5761 gttcctctca aaacacggtc atataacaac ggggtggggt aaatgaatga aaaaggtgga
     5821 ggaaacatta ttcaatagct gtcttaaata cttcgttata aaatgtgaac aaataaaaga
     5881 catctatccc tattttctac acttccactt gactaccatc tttgaatcgt acaattttct
     5941 taactcttct cgtttacctc aattcattct caagtggaat caactcatca tctaacattt
     6001 tacgccatgc tcgcttcatt tcttgtgggt atttaaatgg cttcgcttct ggatgtttgg
     6061 ctttatatcc ttcgtaatcc ttactgttga taattgcttt catattagga attggatctt
     6121 cacctggtag atattgcaga ttagaagcat gtaattcttt ctcagaaaca aaccactcat
     6181 tggaaaagac atgaatcaaa tcatcactcg cttgtgtaaa atagcgacgt ttaatgacaa
     6241 agatatcttc attactatct aaagtcccca catcaaatgc atttaaaatt tgtcggtaaa
     6301 cttctttgac tccttctgtt ttattcaatt tagccaaagc tttttcaatt tcttctcgtg
     6361 catctgaact attcgcagca taagttccta gtaattgatc aatataagtt gaatcaatat
     6421 catacaactt agtggtattt tcactataaa attcgatcgt tgaaaaatct tctccttctt
     6481 tttctggttc ttcctctatt aatgaaccct tcacggtctc gtacacgcca atttgttctt
     6541 ccaaaagttg aatttgattg ttcaaagttg gagatttcac catgtcatca ttgtaatcgt
     6601 cataagtcac taacgattca taagcacgat tcaattcttg aaatgctttg acgtactcga
     6661 tacggatttc aagtggcgta tgttcatcaa tggattccat ggatgaaacc tcttttaact
     6721 taccatatgc ttgtttgaat ctttttttgg attcctgata acttggataa atcaaacctg
     6781 attcttcttt tgcttttgaa tacagttttg tcgcatcctc gacattcttc tccattgtcg
     6841 ctggcttacg aaacgtcaca atcaatcctt ttgtcttatc aggatacgta cgattcgtac
     6901 gtgaaaaagc ttggatcaat cctgcatact ctagattacg atccacaaat agggtttgaa
     6961 tcgtaggggc gtcaaaacct gttaatagac gatccactac aatcgctaaa tcgatttgtt
     7021 tcccaaattg tttgaattcc gctctttttc gtgccaaacg attattgatg tctccgttat
     7081 aacgctcaat gtctgctaat gaccaggaag tatcataata ctgattatat tcctcaataa
     7141 ttttttgcat ttcttcttgt tgggcacttg aatctcggct attttcttct aatgaatagg
     7201 taatagcgat tcgtggaaaa tcaggatcct ccatcgttcg tccttcacga atgagctgat
     7261 tgggaaactc ttttgttaac caatcaggct ctttcgtcat ctctttgatg gcccgataat
     7321 actttttggc catgtcaatg gaactcgtgg ttaagattgc agatttcgtt ggcctgccat
     7381 ttcgaaaatc aaattttgta taggcattat ctggacggaa cattttatga atgacttttt
     7441 ggatatgttc atctgcttca aaaacagctg ggtccaaata actttctttt ttgacaggtt
     7501 ccatttgatc aatcatacga ttgatctgtt ctgatgaata ctctgcatat gtttctactt
     7561 cttttaattt ccgatagatt ttattctcta gtgaagtcgg ctccacggtg ttttcatgct
     7621 ctacttgaaa tcctaagaca gagccatctt ctagcgcatt cttaatggta taggtatgca
     7681 agacctctcc atactgatca tgggttgtac gtgccaattg ccctttggct tgtttccgat
     7741 tctcaggaaa aatcggtgta cctgtaaagc caaaccaagt ggatttcgga aacatttttt
     7801 tgatttcttc catattttca gcacttaacg ctcgatgaca ctcatctact acaaacacga
     7861 tatgttggcc cattaatttt tggaagcgat tggttccttt tttctcttct tgtttctttg
     7921 catatttcaa cgccgcatct agtttttgac gggttgtaat gattacaaca tttgcattgg
     7981 catctgccaa cagggtttca cttaattctt tggcacttcc cgttccaacg atcaagctat
     8041 tcgctttggc gttcccggaa gaaatcccag tattaaattc cgaagcaaat tttgtaaact
     8101 ctgtggtggt ttgattatct aaatctttgc ggtccactaa catgatcgtt cgatcgatcc
     8161 ccgattttcg tgctaaaagc ttagtagata caaaactagt caacgtcttt cctgaaccag
     8221 tcgcatgcca cacataccct gattgatgtt tatttgcggc aataaacaag gcttgaatcg
     8281 catgtacttg ataaggatgt aacaccatga gggttttatt atcttgatct tcactgacga
     8341 ttgtataatc cgcaatcaaa cgatgagcat ccgggatatt taacacttgt ttacaaaact
     8401 catataagtt ttctactttt tcattgtctt tcgttcgcca actaaataaa aacttagggt
     8461 gcaagtcttt cggtaaggca tttgcaaaat accgagtggt ttgttcattg gaaaccacaa
     8521 acaactgtaa ggtcgaaaag atattgttcc gaaacatacc ttcttctgca tattttttga
     8581 tttggttaaa tgcctgataa acaccatctt ttgcagttac ttgtttcagc tcgatttgga
     8641 caatcggtaa tccattgatt aataaggtga cgtcaaaacg tcgatcccgt gcttctatgt
     8701 tacttccacg ttttgcaatt tgatggacca cttcataagt agagattcct ccaccaatgt
     8761 cttgattgga ataaaggata agtgagacag aacccaattg cgaatcttcc cgttcaatcg
     8821 tgatccgagc catcccattt tcacctttta accatttcgc agcatcaaat ggggttttcg
     8881 ttcgcagcag taattccgtt ttgatcgttt caaattcttt atcggtcaaa ggaaaatcat
     8941 tcagttctgc ttgattattt gagataattt tttggcgtaa attgacccaa aggtcttctt
     9001 ctgattttag atccggacga tacgtccact gattatgccc ttctccaagt acacgaatca
     9061 actgttcttc gacttctgct tcctcacggt gactaatttt cattcttctt ccctcctaga
     9121 caaacatctt ttgtaagaag ctttttttcg tttcttttag taaatctaac ttacgttgat
     9181 gaagagcgat cgtatcatct agctgtttga agaaagtacc gattttttgt tgttcatcct
     9241 ttacaggaac cttaacactg atattctcaa tatcagaact gttataagaa ggcaatgccc
     9301 ctacttggga gtatcttggc aaatttatag tttcaaacaa tattttacca aaatagatat
     9361 cccaagtatt attaaataaa taagccatgg tattgttatc tattaagaaa gggcttgtga
     9421 ccaaacgttt tctattaagc attattgctg cgccaacttt agcaaatata atcgctggca
     9481 catcttttat tggcttccat tttttacgct caatttgctc attagataca taatgatttg
     9541 catttctcat ttcatactca ttaccaatgt tattcatgtc ggaaacttta aaaaatggag
     9601 taccttcaga accaccttgc tcggcatcag gaaatccaat tcccgattga gttttaccta
     9661 attctcccaa cttacgctct tcccaatctt ccgtaaaacc aggaaaccgg atttctggca
     9721 cttttgctcc gttttcaggg aacatttttt gtaagaatcc ttttttcgtt tccttcagca
     9781 aatctaactt acgttgatga agagcgatag tgtcgtcgag ttttttgaag aatatgccga
     9841 ttttttgttg ttcttttaaa tcattgggaa tatagaatac tgtatctgaa acttttttcc
     9901 agtctgaacg aggcattttt gttccagatg tatcatttgc agccttttga tatctatcag
     9961 cttgaataag agaataaata aagtccgagt cgctcttctt agctctaaaa acccagaaat
    10021 ccccaatcgc tacgcctgta aaagttgcct tgagccaatt ttttaaatat ggacgtaact
    10081 ttccatatag aatatcattt ggtaaaaatt caatcccttt tctattatca aatttctggc
    10141 tcacatcttt attcagtcta ccttctcctg ccacgatatc ttcaaattca acttttggaa
    10201 gtctactgga gtttgtactt ttattaagtc taacaactaa atcaattaac ttacgctctt
    10261 cccaatcttc cgtaaatcct gggaaacgga tttctggtcg tgtatcattg ctcatgtttg
    10321 aacacctcca gcgcaccttg taaccacatt tcgttttcag gtgttgtttg cagggaagag
    10381 atagcttcta aaagttcttt ttctagcact tgctgttctt gtcgtatctt cttgatgtca
    10441 tttccgacat gaaccatatc aattggttct tcttcttcga aagtatctac atagcgtggg
    10501 atatttaagt tatagtcatt ttctttgatt tcatcaaagg ttgccaaatg agcgtatttc
    10561 tcgacatctt ttctttcagc gtaggtttcg aggatttttt gaatgttttc ttccgatagt
    10621 ttattttgat ttttcccttt aacaaattca cgactagcat caatgaataa tacatctcga
    10681 gtttgacgat tcttcttcaa cacaatcacc gtcgttggaa tcgatgtccc aaagaacaag
    10741 ttcgcaggca taccgatcac agcataaata ctgccatctt ctagtaattt ttgacggatg
    10801 accccttctg cagcgccacg gaataaaact ccgtgtggta agacgatggc catggtacct
    10861 gtttccttca aatgatagaa cccatgaaga agaaacgcaa aatctgcttt ggattttggc
    10921 gctaatttcc cataacgatt aaaccgagaa tcatctaaga aagtcgtatc tgctgaccaa
    10981 ttagccgaat aaggtgggtt cattactact gcatcaaatg tgtaaggttc atctgttggc
    11041 cagtccttat tcaacgtatc cccattgcgg agattcattt cttcggcgtc gacaccatgt
    11101 aaaatcaagt tcatttttgc tagattgtat gtcgttgtat ttagttcttg cccatgatat
    11161 ttaacgttat ctgggtgtgt taagtagtta cgtacgttta acatcaatga acctgaaccc
    11221 atcgttggat caaaaacact aaagaatcgt cgttcctttt gatcgagcgt tacgatttgc
    11281 gccatcatat cagaaaccat atgaggtgtg taaaattctc cggctttctt ccctgcttct
    11341 gaagcaaatt gactgatcag aaactcatac gcatccccga tcacgtcccc atcgtgttct
    11401 aaaacatcta catcattcaa tttcttgatg acttcggtaa tcgtcacatt tcgttgttgc
    11461 tcatctgttc ctagtttttt tgattggaga tccacatcat caaataaccc attaaattga
    11521 ttgtaggtag atgccaattg cacaaaggct ttgtttagat cgttcaattg gaacgttgct
    11581 tgttttgctt gatccgctaa gacgttgaaa agatactctg gtgcaattgc atatcccaaa
    11641 atatcaacga ttgtggcaat cagatcttct tttgattctt catctgaaag taattcacga
    11701 tacaacattg tttgtttgct tactgtgtca tattcctcta aggattcatc tgctaaaaga
    11761 accacttgtt ccaacaatcg atcggataaa tatttataga aaatcagtcc caataaataa
    11821 tttttatatt ctgacgcatc cattttacta cggagattat ctgccgcact aaataatttt
    11881 tgatttaatt ctgcacccaa aaagatacac tcctatctct tatttaaagt tgctatatac
    11941 cctcttattt tagcataccc ccttttataa aaagcaaaaa aaaaagaaag aaacttattc
    12001 ttgctaataa actacgcgaa aggagtctaa cgttgtttga tataacttag actgatcgtt
    12061 aacttatttt ttatatttct acatagttat ctttagaacc atttcagata catatgaaac
    12121 aattacatat gattttttca taaaaaaaca gtactgtcta gtagtataaa atatacaaat
    12181 ttcttaaaaa ttttttcact aaaatcacta cctgaaacag tctaaaacac ttatatatca
    12241 acgtttaatg aaactatctc cgtttcattt cagatacaaa taaatatagt cgggctccaa
    12301 atccgaaaaa aaataccccc agtagcttat cctaagctac tggagatgta taagtacggt
    12361 catataacaa cggggtaggg taaataaatg aaaaagatgg aagaatcatt attcaataac
    12421 ttttttaaat acttcgttgt aatcagttac ctgtccgttc ttaaatacca cagaataatc
    12481 tttttaccca ttttcaagta accgaagtgg aatgtcttta ccaacttgct ctgtcactgc
    12541 attactgata cctgttagat tagctaataa atctgataaa gattgagcat ctgtagaaat
    12601 ctctgtatca gtatttatcg tgattccttt atcttcattt acaagcttcc aagtaggtgg
    12661 tatagctgtg tatgtttcga tgaatgaatc aattctttca tctatcgtct tcccagtatc
    12721 tacttctgtc tcattactct tagatgaatc gattctttca tccatcgtct gctccgtaac
    12781 tgcttctgtc tcattattct caccggaaac tctcttcaca cccaaactta tatattgtcc
    12841 atctttagtg tgcatagtta aaattcccac gcttttacta ataaattcac tataaccagt
    12901 tctttcaata gagacttcaa tgtatttcac actatcatac tcaaaagaaa aatagacatg
    12961 taaaagcatt ttgttgttta catctacata ataatccttt gactctactt gagaccttcc
    13021 tatacggaat acagctttac tgtctgatat ctctatacca gcatccttat tattaatctg
    13081 ccaatctcca agtagctctt ctggcaagct agtagtttct tgtgaacact ttaccacttc
    13141 actggtagag gtcgaaacat tcacctccgc actgttagaa gaattttcca aggaatcagg
    13201 agccttagca caaccaacaa gtctttatat attgtgcagc cgatattttc ttcattcagt
    13261 acacccttga aatatgcagt gttagataaa gcagttcgtc ctgatttgct gaggcaccca
    13321 tctcttcttg aacgtactcc ttgattttca aggccgcccg atactctttc ggaaaacgct
    13381 gcttcactga agccagcaaa aaatcatcct tctccggcaa ctcttccttc ttaatcattc
    13441 gggcaataaa aaactgtaaa tgcgtcacaa atcttaagta aagcatcgaa tctttatcaa
    13501 cctttttttc gggaaacaga tatcctagca tagccaagat atcctttagt gctcttaccg
    13561 agagcatggt ttcttccata tttgcttgtt cactttgggc atttacaatg tgaaacgcaa
    13621 tgtttccagc ctcctcttct ggcaactgaa cattcatttt tttgttgatc aattggatag
    13681 catagcagcc gatctcaaat tcttttgggt aatacttttg aatttcaatc agcaggcgat
    13741 tttgaaaaac aatgcctttt tgactgcgtt caatggcatg cttcaagtga tcaaccaagg
    13801 taaaaaatag acttttattc agcttaaagt caaacttggc gttggcataa tcgataattc
    13861 gcttgacgca aataattaga tcatccggag cagcctcaaa cagtttaatg taatcctttt
    13921 ttagagcatc agaaagtgaa tctaatttaa atactttttg gattttgtca gccggaacca
    13981 aatcgccttt ctttagttga aatccaatcc ctttccccat caaaattact tctttgcctt
    14041 gcttatctaa gcaaagaatt aaactgttgt ttaacgtttt aattactctc attagattca
    14101 ccgttctctc taagctaagc catttcttgc gattactttt tgataccaaa agaagctttc
    14161 tttttttatt cgttttaatt ctttaagatt ctcatcggtt cggtcaacat atataaaacc
    14221 ataacgctta ttgaagcctt gatgggtact aacaacatca atcgctgacc aaggtaaata
    14281 accaaaaaca tctacaccat ctgttaaagc tgcttgaata gccagaatat gtttggctag
    14341 ataatcaatt cgatcctggt cattaattcg tctatcttcc tcaactatgt cgggtgcccc
    14401 atagccattt tcagtaatta aaataggtaa atcatagcga tcgtacaatt tcctcaatgt
    14461 taatcgtagt ccagtagggt caactaccca gccatatggc gttttatcta cataaggatt
    14521 ttcagctgcc cggtagacac cttcttctcc caacatgatc tgttgatccc ccgctctagc
    14581 ggaaacatct gatccgtcat ttttgctggc agcgatggtg gcagttgaat aataattaat
    14641 cgcaataaaa tcgggtttac cggaggataa aattgcctga tcctctgcct caattttagg
    14701 atataatcca cgatcattca aatagttttc aaacaaataa ttgtattttc cccgaactgc
    14761 tacatctaag aaagaccagc ctctcaatgt ttcccaatta tgggctgcaa ttgcatcttc
    14821 tggactggag gtagcttgat acatagccgt taaattgatt gctggaccga tttttccttt
    14881 aagattcaac tgatgataca agtgaattac tctagcttga gctaataaaa catggtgatt
    14941 aatttgaaag agttcttttt ttgaaggcag ctctccactt ggtggcaaac caatcgctcc
    15001 aggatgaagg atcatcgtgt tttgctcatt aattgtcagc caatagttca ccttgtcacc
    15061 atactcagta agcaatacct gtacatagtc cacaaaataa tcgatagttg ctcgatttaa
    15121 ccaaccgccc tgctcctcta gaaaataagg taaatcgaaa tggtacatgg tgacaatcgg
    15181 ttcgattccc tgattcaggc attcatcaat tacttgatga taaaaagaaa ttcccttttg
    15241 attggtttta ttattttcaa tatccataat tcgcgtccaa gcgatggaaa agcgaaaagc
    15301 ttttaagcct aattctttaa acagctgtat atcttcttta tagtgatggt aaaaatccga
    15361 ggcaacttca aaattagcta ccccttctgt atgctgatac ttgtcaataa tagacggttg
    15421 tttcccatcc tcctgatagg caccctctgc ctggtaggca ctaatggagg caccccataa
    15481 aaacttctct ggaaatgctg aaagctgttt gtattgcaca aaaatcctct ccttaactaa
    15541 tttgcatcaa tggttcgtgt tcctttaagc ttccgcttgc ttcactaatt cctgcgtaat
    15601 tttcactgtt ggtcactacc acaatcactg tatcatcgta gccagcgttt ctaatggcag
    15661 cgaaatcggc gttgattaat aattgtcctg ctgacacctg atctcctgct tttacaaata
    15721 cttcaaaggg ttcccctttt aaattcacgg tgtcgatccc cacatggatc aataattcaa
    15781 taccttcttt agaacgtaga ccaatcgcat gtttagattc taaaatagta atgacttctc
    15841 cctcaacagg tgaataaatg ctgttttctt ctggatgaat ggctacgccc tttcccaata
    15901 gtcccttaga aaagacagca tcgttcactt cagaaatagg acacacggtt ccttttaccg
    15961 gattttcaac aatgactgct tcggctattg ccgtttgctc tgattcatca gaagtatcgt
    16021 caaatccaat tagataagca atgataaagg ttgcaacaaa ggcgattaca cacgtaataa
    16081 ttgcgtgaac aatattcatt ggattttctc caataaaggc tggtaaggct gctaagccag
    16141 gggaaacaaa ggcatatctg actaaaccgc tcaagccagc atacaaacca gcaattgcac
    16201 caccaatcat ggaagcaact aatggttttt tcagcttcaa ggttactcca tagagtgaag
    16261 gctcagtaat tcccattaaa gcagtgatcc cagtggacac agctagctgc ttcaactctt
    16321 tgtttttagt tttcgcggct acagcaaagg tagcacctga ttgagcaata ttagaagcca
    16381 gcattcctgg cccattgatc atttcagaac ccgtactgct caattgcata gtagcgattg
    16441 gcgtcattgc ccaagctgta cccgtaacca ccaaaaatgg ctgcaaacca cccatcaaaa
    16501 atggaattaa ccaagctgct ttgccgttaa tccattcagc ccccattgct ataagatcgt
    16561 tcagcaacgt tccaaacggt ccaatgacaa ccaacgctaa aggccccata attaaaaata
    16621 ccagcatcgg cttagtaaag aatttaataa acgaaggcga aattcgatcc gcaaagggct
    16681 ctacatagga catgatccaa acagtaaaga taatcggtaa aactgatccc gcataatctg
    16741 cagctaatac cggaattcct agaaaagaaa cggcatcgcc agtagaaaaa agctgtgaaa
    16801 tatttggatg aagcaaaatg ccagcaacag taatcgccaa aattgggttg acatcaaact
    16861 ttatggctgc cccataggct aatagtactg gcataaaata aaaggctgca tcagcaatag
    16921 tattcaaaat gatgtaagtt tgactactgt ctgatagtac accagtcaaa gtcaagacgg
    16981 ccaaaatggc ttttaccatc ccggcacctg ttatagctgg aatcatcggt gtaaaggtgg
    17041 tagaaatgac actaatcaat cttgaaataa tactttcttg attgtctgaa gaggggccct
    17101 ttttaattga agttcccact tccttttgaa tttccttgaa ggctgtttgg acttcattgc
    17161 caatcaccac ttggtattgt ccaccgctat ccaccacact aattacacca agtgtttttt
    17221 ctaaggcttt cgtatcggct ttccttttat ctttgagttc aaaacgcagt ctggtggcac
    17281 aatgggttaa gctgaccaca ttttcttttc ccccaacctg ttgcaataca tttcttccta
    17341 cttctctgta atccattatt ctctctcctt aaagcagtaa aaaagaccaa gtcaaaccac
    17401 gccccaatca acattgaagt gttagtttgt cttggtcttg cctgcataat cagtaacatg
    17461 ccaataacgt tattttgctg taatccaata atagcacatt atgatagcgc aatcaacagg
    17521 gaatttaatt ttttatcttt tcgtttgacc gtatagggta atataacagt tagatcacat
    17581 caactagggt atatctgcgt atacttactg ctaaaataag tatatctaat attaactata
    17641 ccagtaaaac cattcctaca taaccagcgg gaaattccca aaatcgtgaa agcatcaaaa
    17701 atgcatataa cggatcacac aacttcaaca attctgctct tgggaaaaat tgatgaattt
    17761 ggtgagtaaa ggatggaata aacagcattc caacaatata ggcaacaata gtgatacttg
    17821 tttttttggt aaaaatcagt aagcaatagc caatttcaat cattccgaga tataaaggta
    17881 tcaatagaat gatttgggct agtattctta aaaattaact agcaaatgtt taaactgatt
    17941 tatttctaat ctacatgtag taagtatttc aattccacca attatcaata aaaataccaa
    18001 acatatccat aataattttt tctttttcat ttcttttata ttgttataac agtgaataac
    18061 ataaaaattc ccgacaatca ttgtaatatt tttttgtcac ctgtattagt atttaattta
    18121 taggaaacta taaaaactcc ctagtgaatt tacacctttg ggagttcctg tttggacggt
    18181 atgcataata catttaaatc aatttaacat taacacctca gcatatggta tctccacata
    18241 tatacggtct ttctttggat agtactcaat atttatgttg atagcacttc cattgtctaa
    18301 tatagcttta gcccggtatg tgttatctcc attttcatga gtaatcgtca catcatcaca
    18361 tgatcgattt aagttgtact gttcttgtaa aatctgtgtt accaatggtt ttgatgactt
    18421 ctctaatcca gatgtacctg tcatttttga aaaaataata cctataatag ccaagacaac
    18481 aatacctata atgacaaact tcttgttcat atttacttta ttcaactttt tatctcctat
    18541 ctccaaaata atttgttctt atccctctta tcagtacatt gacatcccta aaagtacacc
    18601 aaagattgct ccagctaccc ctgctatcat caaaatcgta cccattttct catctttttc
    18661 tcgatataag tagcccataa taacagcaac agcaccaaat acaacaggaa taaatgctaa
    18721 cgatactacc atagaaatcc aaccaataat caaatacagc ttccctgtct cttgtccatg
    18781 tacgatttgt tccttcattt cattaattgt ctgctcagta cttttcttta ctgtgtctgt
    18841 tccggttgtc tcatctgttt cttgtagtgc tccacaaaac tggcaatact tactttcatc
    18901 tgttatttct ttgccacatt ttctacaata catgtcttct cctcctaaaa tcattcaatt
    18961 gcttattagt taactgttta ctttattaaa tacatcagaa aacagctcat tcattggtac
    19021 acttaatatc tcagaaagca tcttcatttg atagggttta ggaatcgttc tccctgtttg
    19081 ccacgaactt acacattgct gtgtaacatt catttttttt gctaactccg tttgagaaag
    19141 tcctttttct tttctgtatc ggctgagatt acttactttt ttcatgattc tcccctcctg
    19201 ttttattata tacaacaata tgatgtattt caactcataa aatataaaaa acttcaaata
    19261 ccattacaaa tacaacaact tgttgtacta tactgagatg ggggtgtatt aatgaaacag
    19321 aaacaatatg caaaggtcat tagagaaaag cgaaaagctc tcgggtggac acaaaaagaa
    19381 ttagctgaaa aaatcttctc tacacaacag gcagtagcaa gatgggaaaa ttctgtaaca
    19441 gagcctaacc tcgatagctt aacagctctt tctagagccc taggaacccc tgtaagccat
    19501 tttctagaca atgttgtcgt agattatgag gaagagtttt tggctctcta tcgttctctt
    19561 agcactgaag atgcagttcg aacaattgac tatatgaagc tattaaaaag gcaagaaaat
    19621 gaacgcaatc agctattgaa agattgaaaa ataaataacc caccgaactt taacggtggg
    19681 tgtattgaga tgaaataaga ttaagagcta tggataacct gtctagttta atacaattgg
    19741 gtcttttctc ttccttactc tccgtccatc atctcttgtt acacttgtaa attgatacag
    19801 aggaactatc ccagctcctg ataatatcaa ttcttttatt gtttcatcta tccctgcccc
    19861 tttcgctaaa tatattaaaa aactactact tgctttcaca ttgtatagtt tcttaatatc
    19921 cttagaagct cgttcaagag tgaatccttg tttaacaaag tagcctctgt tttttattaa
    19981 agcatttttc acgctgttat ccccaacatt acgtaggcgt tcacaaacag tgttaataag
    20041 ttctttctct gtcattagat atgtcccttg ccacgataag ccctgtaaaa cttcatgtgc
    20101 ttcatctaaa atcaattgtt tttccttgct gagttctgat cgggaatttt gaggaaatac
    20161 tcttttagcc tctttttcgt taaaacatat cttaacccat tcataagaaa aatattgata
    20221 tgtaaaatga attttttcca gaagtggaag gatatcttca ttcaattttt gaatcacaga
    20281 agttgcatta aaaacgtcta atcgatagac attgcttctc ttatgattaa agtttcctaa
    20341 cgctttctct tcctttaacc tatttaggta ttcgagcatc ttcaaaggaa catcttcatc
    20401 atctaatttt tttatcaatc ctataaaagt cataagtgat agtaacttat tacatttatc
    20461 tatgtcataa gttttaccat agtaagaact cattttttta gctaattgtt cccccgacaa
    20521 aatacaagtc acactttcct tactatcatc aaaaaaatta acatcagaaa gcaagacatc
    20581 atagacacgt cgcatttcct ctagataatt ccttattgtc ctgaaaagtt tgtctttcat
    20641 tgattcaaga gtgtcaaaaa gtacttttga ttcctctttc aattttcgaa aatgtgtagg
    20701 atacaagtct aggttctcta atagccaatt aaatgtttct acccgactgt ctttctttaa
    20761 aattagcatc acaactccaa taaaattttc actgatatca caattactgg aatgacaata
    20821 aaaaatggaa tgcccagttt tactggtaaa aatactagca gagggattat gatcgttgtg
    20881 aaacagacat cttaagtttt tacttgactt aagtaattta ttcatatcaa tggttaacaa
    20941 tctttcataa atctcattaa aagacatgtc ggaggaatca aacaggcagt ctttaaacca
    21001 ctgtttcatt tcttctttgt cgtcattttt tatcaactta acaaaatcgg tttcattgac
    21061 tagttcatac tctctccctg aatcactttg acaattttta gaacatgtca tggtaaaatc
    21121 aatcggaagc tcagtaatat ctaacatgtt gtcttcatta attattatca ctgcatctga
    21181 cttatatcct ccaaaaaaca ttctattcga agcatttaca ttgtcatcac acccggggat
    21241 gttctttttc aagtattcgt aaacagcaac cattttacca tagtcagtaa ttgcttcttt
    21301 taatagaaag acaatcttgt atttttctag attttcagag taccgaatac ttttttgtat
    21361 cgcaaacgca gaattatgaa ctaaattgat agataaaacc ttttctatgc ttaagtatct
    21421 actatcatct ttactcaaat ctactacctt ccccttctca tctttaacag tgttatcgac
    21481 atccagaata attactctgg tttccacaaa actctccttg tcattgcctt tattctttgt
    21541 cttgcttgga ataaatgatt gtgatgaatt tagtatctct acaaactcat ctcttgtaac
    21601 ttctctatag ttatctaaac acctatctct tacatcagtt ttttcttttt ttgtaactgg
    21661 cttctctaat aatttttctt gatctacata aattggaata ttaatcattg atgtttccat
    21721 ttgtgtttcc tccttataaa attctgttaa agactaaaac atacagataa taaaaacaaa
    21781 ctctttatta ctatatgttt tagtcttatt tgtttactca acctttatac cgttccatga
    21841 aactatcaat atcagatttt ttaaatctaa tgaccgtatc aatttttata acaggtaacc
    21901 cgtattgaga aacatatttt ctaatcgttc ctactgatac acctagatat tcacttgcct
    21961 gctgatagtc catatactct agagtgactc cagaaataat gtcttctagc ataggcttta
    22021 gcactgtaat tatctctttc acttgttgtt caacttgtat agaaacaatg ctatcaatga
    22081 tttgtagaga catatttccc cctcctttcg tgattgcttg aacaaatttt ctaagaatta
    22141 tcttcgtatt ttttgctcaa aataaaagta ttttgttatt aaccgattaa tcaaacaaaa
    22201 aatgattacc agttcatcga catacacata atacctcgat aaaaaagggg tgtcaacagc
    22261 tttgtaaaat ttatttacaa cacccctttt ttgaggtata ctaattttag gaggtgtggt
    22321 caatatgata aaacgtccac caataaatta tttagaacga aaaaagatac tcggaacaaa
    22381 aataaaagct ataagaaaga gtaaaaaatt aacacaacca gcatttggtc ttatgattaa
    22441 caacggtcaa ttaattgaca aaaaaactat ctacgaatgg gagaagggaa cctatttacc
    22501 aataccagag cgattatccc gaattgctga tttgggaaat atgagtatag aggagttagt
    22561 ttgtggcaat gtagaagaat atattttggg aattattctt tatagagata gcattgtact
    22621 agatggtata acatttccag ataaaaacct gttccagcat cttcgtcaac agttcccacc
    22681 agtacactct aatttagata catggctgga tagatattcc aaattagaac cagagatgca
    22741 agaatttatt gctaacaaga cttgtaataa agttaaaaat gaaaaaatta gtcttttcaa
    22801 tatcttaaaa atagaagaat tattcatcaa tgctattgta gaagaatttg acaataacat
    22861 tcttttcctc actagcagta tagaagaact actagaaaga atggtagatg aatggctacc
    22921 tattcaattg aaagacatgt cttatcccga agaagctgtg agagaaatta ctgataatat
    22981 aaacaagcta gaacaaacga tttcatcaat tgggaaaaaa tacactaaga aaaaaatgaa
    23041 gggaggtgat accatctagt ggtaagtact ccttgtaaac taagaggaga tgatttaatg
    23101 gcaaaaatat caaagtacaa aaagaaaaac ggtcaagtgg cgtggatgta taaaggacac
    23161 attgccaccg atccacgaac tggtgaaaaa ataaatacta cgagaagagg ttttagtagc
    23221 aaacaagaag ctcaaaaaga ttatgacgag tacagacatc aaatattata cggtgttaaa
    23281 aagaaagcac ctgatatgac atttgaagac ctgtataatg aatggataac tcatcagcgt
    23341 accagtgtta aagcttcaac aatcgcaatc tcagtaagat acgcaaataa tcaaatactt
    23401 ccagcatttg gtaagcttaa actatcgaat atctctgtcc catattgtca aaaagtggta
    23461 gatgaatggc acagtaaata tgaaagttac gattatatga gaaaacagac tgcacaaatt
    23521 ttacgctacg gggttgcaat gcaatatatt gacaataatc ctatggaaaa aacacttctt
    23581 ccacgaaaaa aagagtatga gaaaaatcgt aaattttact caaaagagga gcttaataat
    23641 ttactggatg cttttaaaga ttttggtaac atgaaacaat acgccttctt cagattattg
    23701 gcatacacag gtatgagaaa gagtgaagta ctcgctttac agtggaaaga catagacacg
    23761 tttaataaag aactacatgt caataaaact cttgctgtag atgaatttgg taaagttata
    23821 attcaatctc cgaaaacaag agcttctaga cgagttatct ctttagatac tgagactctt
    23881 tctatcctca acaactggaa attacagcaa aaagaagagt atttaaaact tggatacaat
    23941 actagctcta aagagcaaca tgtttttaca acagttaaaa acactctata cattccaaac
    24001 actgttaatg attggttaag atatattttg aaaaaatata acttgcctcg tattacacct
    24061 catgggttta gacatacaca tgctagttta ttattagaag caggagaatc tgtcaaggta
    24121 gttcaacaac gattagggca tgaaaatagt aaagtgacat tagacatcta tgcacatatt
    24181 acaaataatg cacctaagaa aacaggtcaa gactttgctg atatgatggc tcatcagtaa
    24241 aagagctggt caaaatctgg tcatttctag tatacaggca aaacaaaaaa gctagaaccc
    24301 ttatatatca agggttctag ctcctataac aggtttaccc aacactgcct tccatctcat
    24361 aactgatcaa tcgattgagt tcgactgcat attccattgg aagttctttg gtgaatggtt
    24421 caacaaagcc catgacgatc atttcagttg cttcagattc ggtcaggcct cggctcatca
    24481 gatagtagag ttgttcttct gagatttttg agactttggc ttcgtgttct aaagagactt
    24541 ggctgttgtg gatctcattg aatgggatcg tatcggattt cgaccattcg tccatgatga
    24601 tcgtgtcaca ttcgatatga gagatcgaac ccgcactgtt tttggcaaag gttacttgtc
    24661 ctcgatagtt tacttctccg ccgtcttttg aaattgattt agagacgata gagctagatg
    24721 tatttggtgc attgtggatc atttttgcac ccgtatcttg gatttggttt gctcctgcaa
    24781 aggcgatgga gagcatggtg cctcgtgcac cttttccatc taagtaaaca cttgggtatt
    24841 tcatcgttgt ttttgcacct aggtttccgt cgatccattc aacggttgcg ccttcgtatg
    24901 cttttgctcg tttggttact aagttgtaca cattgtctga ccagttttgg atcgttgtgt
    24961 aacggcaata cgcatctttt cttgtaaaga tttctacgat ggcggcatgc aggctgttac
    25021 ttgaataagt aggtgccgta caaccttcga catagtggat gcttgctcct tcgtcaacaa
    25081 tgatcagcgt acgttcgaat tgccccgtgt tttccgcatt gatccggaag tacgtttgta
    25141 atggaacatc gactttgacg ccttttggca cgtaaataaa ggtcccacct gaccatacgg
    25201 ctgaattcag cgctgctaat ttgttgtcgg ttggcggtac aagttttgaa aagtactctt
    25261 tgaataattc agggtattct ttcaaagcag agtcagtgtc agtaaagacg attcctaact
    25321 tttcgaattc atctttcata ttatggtaca cgacttctga ttcatattgt gcggaagcac
    25381 cggctagata agctcgttca gcttctggta taccaatctt ttcaaatgta tctttgattt
    25441 tttctggtac gtcttcccag tcacgagcgg gtttgtcact tggtttttgg taatatttga
    25501 tggcatcgaa atcgatgtct gaaaggtcgg ggccccatgt ttgcattggc attttgttaa
    25561 atgtttctag agatttcaga cggaattcaa gcatccattc tggttcgcct ttgattcttg
    25621 acatctcacg gatcacttct tctgtcaagc cttctcctgt actgaaaacc ggttcgacat
    25681 catcgtgaaa cccgaattta tattcttcta attctggtac gcccatgttc tcactcctct
    25741 tccttgtggt aatgatggaa agctgcggta ccatcgtttt ccattgcttt tcctagtgct
    25801 ttccaggcaa gtgtcgcaca tttgatccga gcgggaaatt tagctacacc accaagcata
    25861 gcagcatcgc ctaattggtc gggatctttg acttcttttc cttggacaag ctgtagaaag
    25921 tctacagtta aatgttctgc ttcttctatt gattttccga tgacggcgtc tgtcatcatc
    25981 gaggcacttg ccgtactgat ggagcagcca ctgccgtcaa aggcgatatt tttgatgatt
    26041 ccgtctttta catcgacttg caattcgatc acatctccac atgtggggtt gttcaattcg
    26101 atcttgcttg tcgagtcagt caagcttcca cgatgatgcg gatgggaaga atgatccaag
    26161 atcacttgac ggtataaatt gtctaattta gatagtccca tgttggaaaa actcctttgt
    26221 tgccatgatt gcttcaacta aacggtcggc gtcttgcgtt gtgttgtaga aataaaagct
    26281 cgcgcgagca gtcgctgata cttcaaggta cttcaatagc ggttgcgcac aatgatgacc
    26341 tgctctgacg gctacccctt ccatgtctaa agctgtcgcg acatcatggg gatgtaaccc
    26401 atccaaattg aatgcaacga ctcctgtccg aaaattcggg tcttgtggac cataaacagt
    26461 caatccatca attgcttgta attttggcaa cacataagcg gctagttctt cttcatatcg
    26521 atggatattg tccataccga gtttttgcaa ataatcgatt gcatggccta atgcgatagc
    26581 gccggcgata tttggtgtcc ctgcttcgaa tttccaaggt agttctttcc atgtactgtc
    26641 gtataattcg acaaaatcga tcatttcgcc accaaattca acaggttcca tttcttctaa
    26701 ccattttcgt ttaccatata aaacaccaat acctgttggt ccgcacattt tgtgtccgct
    26761 gaaagcaaag aaatcacagt ctagtgcttg gacatcaatg gccatgtgcg gggcagattg
    26821 agcaccatct actacgatga ccccgccgtt ttcatggaca agttcagcta attcttgaac
    26881 aggattgaag acacctaaga cattggatac atgagcaatg gagacgattt tggttttgtc
    26941 cgtaatcttt ttcgctgcat cctccatatc caggaagcct tcttctgtta gttcgatata
    27001 tttcaagaca gcacctgttc gttttgccaa ctgctgccac gggataacat tagaatgatg
    27061 ttccatgtaa gagagcacta tctcgtctcc tgctttgata tagcggtcgc cgaaggttct
    27121 ggctacccaa ttcaagcttg tcgtcgtacc acgagtaaac agcacttctg ctgtttcttt
    27181 tgcatggatg aacgcccgta ctttttctct tgcttgttca taatcatggg tcgcgcgttc
    27241 tgctaacgta tggacaccgc gatgaacatt ggcgttatcg tgttcgtaat agtacgctaa
    27301 tgcctggagt acttgttttg gcttttgggt cgtcgctgca ttatccagat aaactaaagg
    27361 ttcatcattg acgtcttgaa ataagatagg aaaatcattg cgtagctttt tagcatcgat
    27421 catccattca acttcccttc gattacttcg actaattctt tccgaacttg ttctacaggg
    27481 attgctgtta agacagaacc gaggaatcct cgaatcacta agcgctctgc ttcttcttta
    27541 tgcaatcctc tactcatcag atagtacatt tcttctggat caactcgtcc tacactggca
    27601 gcatgtccag cagttacttc gttttcatcg atcaaaagaa taggattcgc atcaccacgt
    27661 gctttatcag aaagcatcaa gacacgactt tcttgttggg catctgcacc ttttgcccct
    27721 ttcaagatgt ggccgatacc attaaaggtc aatgttcctt tttctcggat cactccgtgt
    27781 tgcaaaatat gcccgatcgt atgaggggct ttattggtta ctcgtgtatc gattccttgg
    27841 atttgtctgc ccgagctgat tgccacgatt ttgacttctg cgtgtgcacc ttctcctgct
    27901 aaatctgagt caaagtcagc gatcacatga ccgtcattca tgacaccaat tgcccagtcg
    27961 acagaagcat ctcgtaaaat gtgcccacgg cgattcatat aagaagtaat attttctcct
    28021 aattgatcta cggctgaata tttgattttt gaaccagctt tagcgatgac ttccacgatg
    28081 atattacctg atgattttgc tacttgttct ttggttgtct ggaaacgttc cagataagaa
    28141 aattcactat gttcatctgc cacgatcaac acatgcttga agaagtgctc atcggatgcg
    28201 ccatcttgaa tgaataagga ctcgagtggt tcatcgatca cgacattttt aggcacatac
    28261 aggaaaatac cgctgttcat aaaagctaca tggagtgcag tcaattggtc ttcgttcatg
    28321 tttactgctt ttttcatata atattcttgg actagttctg gatattcgat cattgcagtg
    28381 aacaagtcgg taaaaatgac tcctttttct gctaattcca caggcagttg ttcaaaaatc
    28441 gtccatgaac cttgttggac aagcacagga ttgtctttca ttgcatcaaa tgccggaatc
    28501 gtgcctgttt cactgatggt ttgtgtttct ttcacatcaa ataacgacca gcgatggaat
    28561 ttcacccgat cgatatgcgg caatggcagt tcatctgctt tcgctagtgc gtcttgacgg
    28621 aaggttctca tccaatcagg ttcgccttta cttaaagaaa aatcatttac cgcgtcaaga
    28681 taatcgagcc agtttttttc tttcattctt gctccccctt acgcttcttc ttctttatag
    28741 tcgattccta attctttact gataccagcg tagccttctg cttccagacg tttagctagg
    28801 tcagcattcc ctgtcatgac tactcggcct tccatcatga tgtgaacgac atcaggtgtg
    28861 atatagttca ataatcgttg atagtgggtg atgatcaatg caccgaaatt gtctccgcgc
    28921 atttcattta ctcctttagc aacgactttc aaagcatcga tatccagacc tgaatcgatt
    28981 tcgtctaaaa ttgcaaatgt aggttctaac atcaacaatt gcaggatctc attccgtttc
    29041 ttttctccgc cagaaaaacc ttcgttcaga taacgttcag ccatttcttc tggcatattt
    29101 agtaaagcca ttttttcatc taactttttc aaaaatgcca taacagaaat tttgtcgttt
    29161 tcatcacgtt tcgcattgat cgctgcacgc atgaattccg cattcgtgat acctggtatc
    29221 tcacttggat attgcatagc taaaaataag ccaagtcgcg cacgttcatc tacttccaag
    29281 tctaaaacat ttttcccgtc aaacaaaatt tcgccttctg ttacttcata gtttggattg
    29341 cccatgatag cggcagataa ggtagatttt cctgtgccgt taggtcccat gatggcatgg
    29401 atttctccgg ttttcattgt taggtttacg cctttaagga tttctttttc ctcgatcgac
    29461 acatgtagat tttttatttc taagacagac atgcattata cccccaaatt ctctttcgtt
    29521 ctcactcatt ttagactaat tgagaatgaa gcgcaatgac cttccttact aaaatcaaat
    29581 atcttttatt tattattaaa caattttgat ttctaaatta gaataggtat aaaaaagatg
    29641 agaaataagc cattacagac ttatttctca tcttttttgt tccatacctt attttgcatc
    29701 agcctttact ggtttaacag agccgcccca tttgtctgtg atccattctt ggacttcttt
    29761 gcggtgaagc acttcaatca gttttttgat tttgtcactg tcttcatcac ctttacgagt
    29821 cgcaataata ttcacgtaag gagagttgtc cttttcaagc aatacggcat cttctaatgg
    29881 attcaaccct gcgccgtaag cgaagtttgc gttgattgca actaggtcac cttcatcatt
    29941 ttggtacgta gaagctaaaa gtgatgggtc gatcgtatgg ttgaacttca agttcttagg
    30001 gttttcttca atatcatcga atgttgctgt ttccagatct actccatctt tgactttgac
    30061 taagccagca tcttgtagaa ttgtgatgat gcgtccccag tctgattcag aatttgaagt
    30121 aatgattgtt gcaccatctt ttaattcgga aacatctttg acttttttag agtacaagcc
    30181 cattggttcg atgtggacag caccggcatt gacaaagtca tatccttttt ctttgatttc
    30241 tttgttcaaa taaggaatat gctggaagta attggcatca atgtcgcctt cttccaaaga
    30301 ttggttcggt aatacatagt catcgaattt gacgatttca agatcgattc cttcatcttt
    30361 caataatggt ttcacatgtt ccaaaatctc tgcgtgcggt gtcggtgaag cgccgacttt
    30421 caatgtcgtg tcgtctgctt tatttgctga atctttgctg tcgctgttgt taccgcatgc
    30481 tgctaatcct acagtcagca aaagcgctgc tgcaaatcca aaaattttct ttttcatatc
    30541 ctacatcccc ctatatccta ttatcttttg tctgttttct tagttaagaa atcaccgatt
    30601 ccttgaacga taaatacgat tagtaaaata attaaagtcg caacgaatgt cactgttaga
    30661 ttaccacgct ggtagccttc ctgccaagca agacctccaa gtccacctgc gccgatcgcg
    30721 ccggccatcg cagtaaatcc gatcatcgag atcgttgtaa ctgttaatcc agaaatcaat
    30781 gcgggaatac tttcaggtat caagaccttc cagatgattt cccagtaact tgctcccatt
    30841 gcatctgctg cttccagtac accagggctg acttcacgaa aagcaatctc tactagacgg
    30901 gcataaaatg gtgctgcgga taaaacaaga gctggaatgg ctgcttgcgc acctagcata
    30961 gtgcctacga ttgctttagt aaatggaatg atcaaaacca tcaggatcat aaatggcgtt
    31021 gaccggaaaa tattactgat gatcgatacg attccataaa tgattcgagc atacggtttg
    31081 tttttgcgtc caatagagta aaggatcaat cccaaaacta atccgatgat cgtcactaag
    31141 accattgaga ttaatgtcat atatagtgta tcaaggatag cctgattcat cgtttccggg
    31201 ttgatttgtt taaaatctag atatgtttct gaaaagcttt tatccacgat ggatcacctc
    31261 aattcctact ccttgttctt tgatgaacac ttctgcttga tctaattctt tggtttcacc
    31321 agtcatcaag acagtcaatg aaccaaagga accttctttc acttgtttga tctttccata
    31381 cacaacgttg acatcgatgt tatagcgtcg aatcgcttgg gaaatgactg gctcgttggc
    31441 atttctttcg ctaaaggtca aagttgccaa acggccgctt ggattttctt tgatgagttc
    31501 agctaatagt tctgtcgaat cagcttctgg ttcgatgtct tgttgtacaa agcgtttggt
    31561 tacttctttt ttcggttgtc tgaagacagt caatacgtct ccttcttcta cgactttccc
    31621 caactccatc acagcgactt tgttgcagat tttgcggatc acattcattt catgtgtgat
    31681 caagacgatg gtcaaattca atttcttatt gatcgctaac aataagtcta atacttcttc
    31741 tgttgtttgc gggtccaatg cgctagtagc ctcatcacaa agcaggatct caggatcatt
    31801 tgccaatgct cgtgcgatac cgacgcgttg tttctgtccg ccggaaagtt gtgaaggata
    31861 tgcttgtccg cgtccttcta acccgactaa acgcaacagt tcttctgccc gttctttacg
    31921 tttgctcttt ggtacgccag ctaattccag cggcagctgg atattttcaa gtacagttct
    31981 tgaccataac aggttgaaat gctggaagat cataccgatt ttctttcgga atgtacgtaa
    32041 ttctttgttt tttaattttg taatatcttg atcgtttacg atcacttcgc cgctcgtggg
    32101 aagctcaagt ccattcagga gacggactaa tgtacttttt cctgcgccag aatagccgac
    32161 gattccataa atatcccctt tttcaacgtt tagtgaaacg tcattgacgg cttggatgtt
    32221 tccgtttttc ccattgaatg tctttttgac attcgttagt tggattaatg acatgccatt
    32281 cacatctttc tcttatgatt tttcaaacaa aaaagccttc gccctcttgc cttaagcaaa
    32341 aggacgaaag cttgaacttc cgtgttacca ccttgattta ccgttacttc gcaataacag
    32401 cctcttccag tacttggact tagcttcttt ccgtatactg tcgcgctgta acgggcactc
    32461 ccgtagtaag tttgcttacg ctcgcttact ctgctctaag accatcttcc atctcatttt
    32521 cgttacccat tttcagcaaa cagggctctc ttcaaacgtc cgtgacatgt actcttcttt
    32581 tcaaagcttt ctcgtttcat ataggattgc ttatgattat agcaactgga agaatcagtg
    32641 tcaacttttt tcaagtgatt ttctgatttt ataattcgtc aaattctttt ggatcaacat
    32701 ctttgaaact taagatccac gcattcattt catctgaatc attcaagata ttgatatctt
    32761 caccgatttt atcattaaca gaagaaactg tacccgttag cgggctggca aattcattga
    32821 ccgctttttc cgcttcaact tcgatcagag gttcatcttt ttttagcgat tgtcctgctt
    32881 ttggcagatt ggcaaacgtg atgtctccaa gttcttcttg cgcttcattt gtcaaaccaa
    32941 cacagtattc ttttccatta aataagatcc aaaggttgtc ctttttcttc aaatgcttgc
    33001 ctgccatttt atatcagttc tccttcataa attttttcat tgaatccaat tgcaacaagc
    33061 tggtcctctc gtaagatcag tggtcgcttg atcagcatac catctgtcga taagacttgg
    33121 ctcgcttttg aagccgtaaa ctcgcctact tgatctttca gtccaagttc ccgatatttc
    33181 atcccgcttg tgttgaacag gcgacggatt ggtacttgac tcgtttctag ccaagtctca
    33241 atcgttgagg ccttcggtac ttctttgatc atatcaacgg tttgataact aacgccatgc
    33301 tggtctaacc acgctttggc ttttttgcat gttgaacatt tcgggtacca gtaaaaggtg
    33361 tacaattttc ttacctcctt gaagatctta caggtgcttc attttggtat ctcatcgata
    33421 atatcaaccc gataccgatc atgttcccta aaatcgagga tcctccctga ctaatgaaag
    33481 gcaatgggat ccctgttaaa ggaagtaatc cgatatttgc tccaatattc tcaaagacat
    33541 ggaacagcag catcatgatc aaaccgcttg cgatataggc gtaaaattca ttattcgtat
    33601 cgaaacacac ccgaatcata cgatagatta aaataaaata caagaaaatg acgaatgcac
    33661 caccgataaa gccgaagttt tcaccgataa cggtaaaaat catatcggac tcacgaacag
    33721 gcacatagat attgcttacg ttgaatcctt taccaaacaa gccaccggta ccaattgcca
    33781 aaagtccttg ggccggctga taactcattc ctgacgtatc atggaaaggg tccagccatg
    33841 aatcaatacg tgcaaactga taagatttga atccaacttt ataaagcaag tctcttccgc
    33901 cttcagtcgt aaccaagaag atcgttcctg ctccaacgac tactgccaag attactacag
    33961 gtgcgatgat gcgccaagag attccagaca tcaggaaaat cccgccaaag atcgcaagaa
    34021 agacaagcat cgttccaaag tcatcttggg ccatgatcaa tgctaaaacc ggtattgtca
    34081 cgatcaagag cttcccaatc agccaaaagt ccgatttcat cgtccgttct tttacttgcg
    34141 tattatgttt agttacgatc agtgccatca tcaagatgta cgcaatcttc atcaactctg
    34201 ctggctggaa agtaaacgca ccgaatcgaa accagttttt agaccctgtt gaagcagcta
    34261 aattctgatc atagaatttg agcaagagtc ccattaccac taaccccaaa ccataaaggt
    34321 aaggagtcag tttccagatc catttggaat tcaaatgcat gatgacaacg attgctgccg
    34381 ctccaactaa ataccagaca acttgcatcc ctacgccttt caagactgct ggattgttcg
    34441 gatcatggct aagtgctaca tataatgaga gcagaccaat caaacataac agaaagactg
    34501 gtaaaatcac gccataatca atacgattat cgttcgaagc ttttaatttt ttttccatgt
    34561 ttcatccctt ttcttaaaaa ccatactgct gattattata gcggtccagt tagataatgg
    34621 aaagtctttc catagacatt tgggaaaaat taactttccg tttgtgcagt tgcttgagca
    34681 aatgcagtac aaaagcctac tgcttatctt tagtgtatca tagaactttt tttgatacat
    34741 gcaattatct accagctgag attctcctcg ctgtgagaag tttctgtgct ttcattgtat
    34801 cgaaggatct cttcggcaat ctctagcagc tgatcagaaa ccagattggt tcgttggatg
    34861 aaataaaaat atcgatggaa attcaacgga gtcattggca cattttctgg aacagagcgc
    34921 ttagacaaaa tcgagcgacc gattccttcc ttcagcaacg caacaattac atcgttactt
    34981 ttcacgatca tcttcttatc ttggatattc atctcttcaa aataacgctg cgtgtaatga
    35041 aaaacaccag agtttgcttc tcttacaagc cacaatttgc tttttgggtc cccagcgatg
    35101 actagctggt cttccatcaa agagtatctt tgtacgccgg aagtctctaa tggcttttcg
    35161 ataaaaccaa aatcggattc atgtttcgct acttgttcta atgattcatg agaattcgtg
    35221 atttcgatct caaaatgcac tttaggaaac cttttgatca gatgtttcat caactgaggc
    35281 aaaagataga cagcaaaggt gtgggaagca acgatccgac atacttcttc ttcattttgt
    35341 tcattgagca atttttgctg agtttcttcc cattcttcta acaaaaccag tgtccgttca
    35401 tataataagt cggcttgctt agttggaagc atctcttttc ttccgttacg ttgaaaaaga
    35461 tcgatattca attcccgctc caactgtttg atttgattcg agactgctgg ttgagataaa
    35521 aacagtactt ctgctgctct ggaaaaattt cttgtttcat atactgctcg aaaagtcgtt
    35581 aataacttaa acatggcacc acttccataa ctttttatga taattactat aaaaactatt
    35641 tgatttaaat atacaaaaaa ccatctataa tagaaatgta caacaaaaca gttgctgatc
    35701 caactagtaa cttagatgaa gacaatcagc tgtctgcttg ctcgtaatca tacattccta
    35761 ttcattttac aatgtattcg ttcatcttgg tactaaaatc tattaaataa aaaggagaat
    35821 attatatatg aagaactttt tgaaaattat tccgatcccg atctgcgggt tgatccttgg
    35881 gttagcttcc cttggaaatc tgtttaaaga ctatcatcat gttgctttag gcaatctaac
    35941 tggcggtatc tctatgattc ttatgatatt catttttgtt aaattgttta tcttattcga
    36001 acacacgaaa caaacgttgc atgatccaat catcgcctcc gtttcaccaa cttttacgat
    36061 gtcactcatg gttatttgta cttattttgt ttcatatgaa agtattgcac caattgtcaa
    36121 attcatttgg ctgtgcgctg ttatcttcca agtgatcctt gtcttttatt tcaattatca
    36181 ccatgttatc aaagctgatc tttcaattga agcaatttat ccaagctggt ttatcattta
    36241 cgttggattt ggggtcatca ctgtaactgc cgggaacttc ttcccactta ttggaaaaat
    36301 tttcttttgg atttcattga tctgctatgc gttcttattg ccgatcatca tcaaacgtgt
    36361 ctttttcgta agaaatatgg ctcatccgac acgtccgctg attacgatcg ttgcagcacc
    36421 tggttctctt tgcctaacag gttatcttaa aaactttgct gaacctaatt tttatcttgt
    36481 cgtagcattg ttcgctttat ctcaagtatt gtatttcgtt gtgctgtcta tgcttccacg
    36541 tttgttcaag cttcgttttt atccaagcta tgcagccttt acattcccgc ttgttatctc
    36601 tgcaacagct atatttacta ctgttcatta cttcaacagt ttaggtatct atagtagcgt
    36661 attggatttc ttaaaagttg cggaatgtct gtttgcaagt gtagttgtct tctacgttct
    36721 tgctcattac gtacgtttct taattaaaga acatagcaaa catggaaaat ttgtttctaa
    36781 aaaaatcgct taataatcga ttcaataggt tgtgaaaaag ctgttctgca tcaagtaata
    36841 agaacggcca aacaaaaaca gctgttcatg tttttgtact ggttgggctt attaccgcag
    36901 atgcaagctt ttgaacaccg tttaccaaga ggtcgtaaaa gaagtggtca gcttcaagaa
    36961 ataagaagga atttacgaaa attgcttctc aaatttttgt gaaatttcgg cttatttccg
    37021 tagaagctac ttctggaaca ccgtttattc gaaacaagag gtcgtacaag acttttgtca
    37081 cgacctcttg ttcttttgtc aaaatactgg tttagtgcta ctatgttcga gtatgctttt
    37141 agtatttatt gacgaaagga accgatcaaa tgaaatcatt gtcacatcca ataaaatctg
    37201 taaaggattc agcggtccgc gaaatcttac ccggtttact gctttctttt gccatcgctg
    37261 ccttcagcag gcttcttgct atttatctgc cacagttagg tggagcgact attgctattt
    37321 tattgggtat tttattagga aatacttttt ttaaacagcc tgttctagca atcggcacaa
    37381 aatttgctga aagcaaacta ttagaattct ctgttttttt actaggtgca actgtcacag
    37441 ctcaaacgat tgctcaaatt ggtgtaaaag gaattgtttt tgtgttactg caaatgtcag
    37501 tcactatcat cggtgcttac ttgattggta aaaaactgca attcagcgat acttcttctt
    37561 tgatgatggc tggaggaaac gctgtttgtg gttcttctgc tattgcttca atcgcccctt
    37621 ccatccatgc gaaagaggaa gaaaaaggac agatcatcac actagtcaat ctactgggta
    37681 ctgtgatgat gttgactctc ccttttcttg gattacagtt gtttggtgga aatctcattg
    37741 aaaaaagtgc acttttgggt ggtacactcc aatctgttgg tcaagtagtc gctggggcaa
    37801 gcttacttga caattctgtt gtcgaattcg ccatgctatt taagattcta cgtattatga
    37861 tgctcgttgt tgttgtctat ctgtttgaaa aattcatgca caagaaacaa ccagctctgt
    37921 ttgaaacgcc aactgatacg cagaaaaaac gaaaattacc gcttccttgg tatgtattag
    37981 ggtttgtgat cttttgtttg gtcaacagct tgttttcttt acctgtagtt attgaccaat
    38041 cggcacattt tatcagcagc tggtttgaga tcactgcttt agcggcaatt ggtctgcgtt
    38101 tagactttgc aaaattcatg aaagaaggta cacgcttctt atcttatgga ttagctgtcg
    38161 ggcttctcca aacagtgatg gctttactat ttatttggct gatcaaagtt tagaatgaac
    38221 atgaaaaaac agttgtgatt ctcgcatgga acaatgtccg aatcacaact gtttttaatt
    38281 ttgcagatat ccttctaaca acaacaattg gcgtttgatt tcaaccccat ctattgcgtt
    38341 tcccatatat cctgtcagtt ttccattttg cccgatcact cgatgacaag gaataacgat
    38401 tggcaatgga ttggttcgat ttgcctgccc aatcgctcgc acagctttcg gactatcaac
    38461 agcaacggct acttctttat acgttcggat ttctccataa ggaatggtcg acaaagcttt
    38521 ccagacattt tgctgaaaag gcgtacctct ctttatagat aaaggaacat caaattcttt
    38581 tctttttccc gcaaaatatt cttcaaactg ttcttgtgtg cgcgttaaaa ttcgctgatt
    38641 cgctgattcg ctttctgaaa ttggctgaaa agatacagca gtcaatccgg attcatctgc
    38701 atctaaccat aaaaagccga taggtgtgtg tattttcatt ttgtttgctc ctagtcaata
    38761 gactggaact tcctcctttt tctctatcca aaactacttg ccaactaata aaaattgttc
    38821 ttaaaagttt gataaaccga ggaaacatgg taaactaaat acgttagttt acaaaaagaa
    38881 tacctgttct tttacgtaaa atcaaatttt aaaggtgtta tttaaggttt attaaatttt
    38941 gaatgtaaac catatataaa catacgattg aaaggataga aacgatgaaa gtatcaattg
    39001 ttgtcccatg ttttaatgaa gaaaaaactg ttcctctttt ttttgatgag gttgaaaaat
    39061 tcaaaggtgc ccatgagttc gaatatttgt ttattgacga cggatcaaaa gacggtacgt
    39121 tagcagcaat caagaagcta gcctcagctt atccttatgt ccgctttgtc tcattttcca
    39181 gaaattttgg gaaagaagcg gctctgtacg ctggattgca agcttctaca ggagatttag
    39241 tgaccgtgat ggatgttgat ctgcaagatc cgcccgaact gcttcctgag atgatcaacc
    39301 ggatcactac ttctgatatc gattgtgtcg gcacccggcg ttcgactcgt gaaggcgagc
    39361 ctgcgatacg cagtttcttt gcgaaaaaat tctaccagtt gatcaataaa atcagcgata
    39421 cagaaatggt cgatggcgct cgtgatttcc gcttgatgac tcgtcaaatg gtggatgccg
    39481 tcctagaatt aacagaatac aaccgatttt caaaaggatt atttagctgg gtcggattta
    39541 aaacagaata tatttcttat gaaaaccgcg aacgtgtggc tggcgaaaca tcttggtcct
    39601 tctggaaatt attcaactac tcgattgatg gtatcgtgaa cttctcggat gcaccgttaa
    39661 cgattgcttc atttatcggc gctttgtctt gtgtcggttc tggtattgca attttgttca
    39721 ttattttgcg ggcattgttg tttggtgatc ctacatcagg atggccttcc atggtctcga
    39781 tcttcctgtt cattggtgga atccaattgt tatgtcttgg aatcatcggg aaatatattg
    39841 ggaaaatctt cttagaaacg aaacagcgtc ctgtttatat cataagagaa acagaaaaag
    39901 tgaagccaaa tgaattgaag cctcaccaat aatatacgtc aaatgaagta aacatttttt
    39961 taaatgaaaa aggactgtga caaaagtctt gtcacagtcc ttttttccga ataaatggtg
    40021 ttccagaagt agcttcttcg gaaataagcc gaaatttcac aaaaatttga gaagcaattt
    40081 tcgtaaattc cttcttattt cttgaagctg accacttcta tcacaacctc tttcatatct
    40141 tattacttct tcattgctgc ttttagtgct gctgctaatg ggctttcttc tggttcttct
    40201 gcttgtgcat attttttcag caagcgtcgt tcttcatgtt tggtcatttt cttcttctgc
    40261 tcttttttat caagcaattt ttctgtcgtt ccatcgtatt tacaacggaa ataagcaccg
    40321 tttttacctt cgatgatttc cattttccgg tgacattgcg ggcatcgatg attagagact
    40381 tttggatctt ttcttctgcg gtagttgcaa tctgaattgg tgcagacata gattttgccg
    40441 tcacgtgtgt ttttctcgcg cagattggcc ccacattctg ggcatttctt ttgggtaatc
    40501 gagaaatctt gatattcttg tttgcttgat ttgatctcat tcaccaagcg ttttgtttct
    40561 ttttcgattc cattcaagaa ggttcctgct tgtttctttc ctgcagcaat agcttcaagg
    40621 tctttttccc agctttctgt caaatctggt gttactaaag agggatttac caatactaat
    40681 aattgcttac ctttaggaga tacttgcagt ccttgagggg tacgttccat cagttcagat
    40741 ttgattagtt tctcgataat ctctgcacgc gtagctgggg ttcccagact gtatttttcc
    40801 atttgaccaa gcaagcttgc ttctgacaat ggtttaggcg gtgcagatag ttcttttttg
    40861 atggcgaaat cgggattgat tctcattcct ttttcccatt tgactgtttc cgtctcagtt
    40921 tcagtttcgc ctttccatcc tgcttggacg acacgatttt gccgaaaaat gaatcgttct
    40981 ttaccaaatt caaccgtaac ttttgtttgg ctgactttat ggggttgagc aaacagtcct
    41041 aaaaaacgag agacaatcat ttgatagatt tttcgctctt cattacttaa tttttcataa
    41101 cgtgctctgt tttcagttgg caaaagtgca tggtgatcgg tcactttact gtcttggaat
    41161 acttttgtct gttggacgat ggctccgttt ttcaaatact gcttgacctc aggagcaaag
    41221 tcgctgactg cttgcaatcg ttctttcatc gtacttttca tgtcatttgt cagatatttg
    41281 ctgtctgtac gcggataagt aacgatctta tgggtctcat acaaactttg tacaagactt
    41341 aatgttttct ttgcagaata gccatagcgt tgattggctt ctctttggat ctctgttaaa
    41401 tcataaggca atggcgcaga ttccgttttt aatttttctt ggacttctgt gacagttcct
    41461 ttgcttgtac tgatcaattg aacaagtttt tgtgcttctt cttgtgtttt taaagcaaat
    41521 tgattttttt gaagcatttt ggcttgttct tttccagctt gcaacgtgat cgtgaaatac
    41581 gtttgtggac ggaactgctc gattttcttt tcttggtctc tcactaatgc tagtgtcggt
    41641 gtctgcacac ggcctgctga taaattatcc gcgtacttca cagttaaggc tctggtcaca
    41701 ttcaatccta ccagccagtc cgcttttgcc cgggcaagag cagattcata caatgcatca
    41761 tattctttcg caggtcttag ttgcttgaat ccagttttga ttgctttatc tgtttgagaa
    41821 gagatccata accgcttcac tggtttattg aaatgcacat actctaagat ccagcgagca
    41881 acaagttccc cttctcttcc ggcatctgtc gcaatgactg cttctgagac atcttttcgc
    41941 tgagccagct gcttgattgc ttttaattga tgtcctgttt taggaagtgg tttgattcct
    42001 agttttttag ggatcattgg caacgtgtcc atttgccagc tctgccattc cttattcaga
    42061 tcttccggca ttttcaatcc taataaatga ccaagggccc aagtaacgat caccttgggt
    42121 ccttcataat aatttttgtg cttttgatta gcaccaagca ccttgcttag gtctttcgca
    42181 acgcttggtt tttccgctag aattagttgt ttcataaaat tcctttctgc tttctactgt
    42241 gatgggctga tgatttttca acaacgcaag tcccttgtat tgctgtaaat catcgtcaca
    42301 ctcttcacac cagcgctcat ctccgtcatt ccaaaaggcg ctgagaaagt tactttttgc
    42361 attgatatat tcataatttt gatggatttc gttccattgt ttttcgtacg cttgtcgtgc
    42421 ttcttcgaaa gactcgaatg tttcttcttc ttcaatatct tcttcccagt cttcaaaaaa
    42481 ccaccaaggc tcattgtccc cgaacatggt aattacttga tacatactcg tgctccttct
    42541 ttcagctttt ctagactcat cttagtcact tttcaagaaa gttgcaagaa ttttctctta
    42601 taaaagggtc atttaatgaa catcttaaaa ttgttacaaa tgaatctgat tgtttaaggc
    42661 aaagtagcga taaagttatt gatcatccaa aaatagctgt cttctggatc gattgttggt
    42721 tcttttgtaa agtatcctaa aaaacgtaag gaaagataac cgtggatcat actacgaagt
    42781 tccctgcttc gatgaacttt ttcttctttc gttaattgaa aagcctcaaa tatctgcaaa
    42841 atgatctgat tcgtttcatg gattccatct aataaaacag cttcattggt atgcggtaca
    42901 ctgatcagta actcatacac tgcttgattt gaaaaagcaa aggatcgata agtttctgca
    42961 tagatctgca gtgcttcctg accacttttt cccacaagag cccgtcgcag ctggtcatta
    43021 agttcctgca gaagtttcgc tgttagcgct gttttgacat cacgaatatt tttgaaatgg
    43081 ttatacagcg agggagcctt gatatccaag cgtttggcta aatgctgaaa ggttatttgc
    43141 tgaacatcca tctcttctgc tagttctcga aagcagtcga tgattttctc ttgagacaga
    43201 tttctttttt tcatttccat gtttcctctc ataaaataaa tccttcactc tttcagttta
    43261 ccacgaaaaa gaaagagaaa aaagagactt tcattgataa actacatatt gtagtttata
    43321 ataaagagct gtagatgaaa attttcagaa agaaggagtc taattggctg caagaataaa
    43381 ggaaaaatta tttaggaaaa gagaaacttg ggaacaaaac ttgtttgtct tatggtttgg
    43441 aacatttatg gctggtatcg gatttagttt agtcatgcct tttatgtcac tttacataga
    43501 tacgttaggt aattatagta cgtcacaatt gaacttttgg agcggattga ctttttcatc
    43561 tactttcttg gtgactacgc ttatctctcc ttggtgggga aaattagccg accaaaaagg
    43621 aagaaaattg atgctgttgc gtgcttcatt aggaatggca atcgtcattt ctttgatggg
    43681 ttgcgtgact agtgtgtatc aattagtcgc attgcgtctg cttcaaggga ttttttctgg
    43741 ttatatcagt aatgccactg cccttgtcgc aactgggacg ccaaaagaaa aaagtggtca
    43801 agttcttgga accttagcaa ctggttctgt tacaggaaca ttacttggac cgcttcttgg
    43861 cgggatctct gcttcagcgt ttggctatcg ccctaccttt ttcattacag ggatcatctt
    43921 atttttcgtc ttcttgttga gtctgttttt cgtccatgaa aaatttgtcc cagtgaaaaa
    43981 agaaaacatg gtttctgcca aacagatttt caaagacttg aaatatccac acgtcgtcat
    44041 cggtatgttc gtcacaacga tgatcatcca agcatccaac aattccatta gtccaatcat
    44101 cagtctgtac attcgccaat tgctgcatgg acatgggaat gttacgttga tcagcggaat
    44161 cattgcttcg atacctggga ttgccacttt gatagccgct ccaagatttg gtcgcttagg
    44221 tgacaagatt gggagtgaaa gaatacttgc gattggtttg ggttttgcga ttctggttta
    44281 tatcccaatg gcattcgtca cgaatgtttg gcaattagct gccttacgtt ttttgattgg
    44341 tatctctgat gcttgtctgc tccctgctgt ccaagcattg atcacaaaat attcgcctca
    44401 tcaagctgct ggacggatct tcagttataa ccaatctttc caagcaactg gtaacgtagt
    44461 agggcctatg atcggctcca gtgtttccag tgcatttggt tatcgtggcg tctttctttc
    44521 gacttctctt cttgttttgg tcaacttctt gcttgtacga cacaatacac aagagatcca
    44581 tcagcaagaa actgcagaag aaaaagcttc aggagtctcc atagcttcag ggcactctcc
    44641 ttctcataga caataagcgt aaatgaaaaa cactgcttga acagttgtca cagactgtcc
    44701 ttgcagtgtt tttgtttagg cctgtttttt caagaactca gataagagga ataatccctt
    44761 ttccaaggtt tcttttttac aacagtaacc tagtcgggca tgcttcggta aatcaaaggc
    44821 acttcctggt actaatagta ctcctgtttc ttctaataaa ttgatacaaa atgtctggtc
    44881 atcttcttcg atggctaatt taatgaaaga agtagagacg taattgggag cgacaagttc
    44941 aactttcggt tcattggcta cccattgttt taaaatcaca aggttctctg taatgatctt
    45001 tttgtttcgc gccaatattt tttctttatt cttcaatgca tgtacagcaa gatcatctga
    45061 caggactccg ccgcaaatca tcgtgtagtc tcgatacttt cgaaaaactt ctgccaactc
    45121 aggtccagta gctgtccagc caatacgaat acctggaatg gagtaggttt tggataggga
    45181 attggttgct ataccttttt catagacatc tacgatcgac aagaattcac ctttatccgt
    45241 taaaggcgca tatacttcat ctatcaatac atatgcatct actgtacgag caatttctgc
    45301 gattttttct aaagttttcc gatctagcaa tgtgcctgta ggattgtttg cactattcaa
    45361 acagatgatt tttgtttcag gtttgactaa ggcttctagc tgttctaaat caaactgcca
    45421 atcttcttct tccttcaaat gaacaaagtc aacagcggca cctaaagatt tggggatgtc
    45481 atataattgc tgataagtag gaagcatcga aacaacatgg tctcctgctt ccactaacgc
    45541 gtatagagct aaaagattag caccagtcgc tccattcgtt tgtaagatgt tttccggatc
    45601 gacggtttga taaagagaag cgacttcttg tttgaaaaca tctgatcctt cgatccagcc
    45661 atagtccaat ggttcgtttt tgcgtttttc gaaaaattta cttacgctag tcccatctaa
    45721 gccaacgagt tcgtctaatg ttaatgcttc gatcgagctt tgtgcaatat catataccgc
    45781 tttcttttcc catttattta gccattcttc tacaccgaaa tttgcgattt tcatgtttct
    45841 ttccccctta tatcttgatc cagcctacag caagcaaccc gattgaaagc agtgcggcca
    45901 cacaaatcag cccaatcatt actttttctt ttcccgtaat ttgatgtttg gtttcacgac
    45961 aagcgatcca gtagaagcaa aaaccaggaa gataggcaat actacataaa aggacataag
    46021 aaaatcctgc taaaatgatg cccatcaatt caaaaactac agcaatccca ccgatcacta
    46081 cttgtttttt gattccttct tttaaagaaa actggacttg atagatcccg acaagtagat
    46141 aacaaatcaa aattgccgct gtacataaag aataggcaaa ttgataggca taatcagtaa
    46201 ctaagaaagt caataagaat agattagtca agattgcagt aacaactaac gaataagttg
    46261 gggcattttt tttattcgtt tttccccaac ctttcggcaa cagaccgtct cgagccatca
    46321 acatcgttgt ttctgctggg agcatcgtcc aagacaacca agaaccaatg atcgaaataa
    46381 tcaagccgat attgatcagc gctgctcccc aatttcccac gattcctttt aaaatatttg
    46441 ccatagcagg ttgtgaaata gttgctaact cttcttgaga taagacgccg tatggcaaga
    46501 tcgaagcaag gatatagata aacaataaac aaagcagacc aatgatcgat gcttgctggg
    46561 cttctgaacg tttcttcgcg cggttggcta aaacacttgc gccttcgatc cctacaaaga
    46621 cccacatcat aaccatcaaa caacctttaa tctgcaacca gatcgttcct tgctgccctg
    46681 ttccattcac tagattatca gatacgttac cccaaaaatc agcagtgaat atattcactt
    46741 taaatgcgat aaatacaaat attaaaaata ggaataatgg cactaattta cagatcgtca
    46801 cgattgtatt gatgacgctc gctccttcaa ctccattgtt tactaataaa gtcagacccc
    46861 aaataaaaat acttgcaaat aaaatagaag gaacattttg tccacctttg aatatcggga
    46921 aaaattctcc catggcgctc atcagcatcg ttgcaaaagc aacatttccc agccacgcag
    46981 acaaccagta accccaacca ctgatgaagc cacctaattt accaaatgaa gcttccgcgt
    47041 aaccgaaaat accaccatct agatctggac gcttttctcc taaatgattc agcgataata
    47101 ccaaagctaa aataccaatc ccaacgatta tccatgaaag gatcgctggc cctggagctg
    47161 cagaactagc cagatcactt gtgatcccaa acacaccgct tccgattgac gaactgacaa
    47221 ccagagcaac cagtccccat ttgctaatac ccgtcgattt cttttccatt tccatcaccc
    47281 tcacttataa aattgaacaa atcacttgca aaagattccc ttctattttg caactgctct
    47341 tgcttaacaa aaagcaactt tattcatatt attggatatt atgaactttt tacacaagta
    47401 tttcaagtat aaaatgaata aaaattaatt ttattgcaaa aaaacagcta tgctatccaa
    47461 aagaaaaggt gtttcttttg ttagccatag ctgttttgat tcatttaata tcgcttaaac
    47521 gtattgtaaa acttttgttt cttgatatgc gtggtcgatc aaaccaccac ccagacattc
    47581 cattccatca taaaatacaa ctgcttgtcc aggagtgatt gcacgtacag gttcgtcgaa
    47641 tacgacttct gcacgattat catccaataa gcgaactgtc acaggaacgt cttgctgacg
    47701 ataacggaat ttcgctgtac acttgaattc ttttggcatt ggttcattcg tcgtaaagtg
    47761 gatttcactt gcatccaagc ttgtggcata caatgcagga tgatggaacc cttgccccac
    47821 atataatgtg tttgtcgcta gatcttttcc tacaacaaac cagggttctt gcgaatctcc
    47881 tccgccaccg atccctagtc cttgacgttg accaatcgta taatacatca agccagcatg
    47941 ttgtcctttg acttcaccat caagagtgac catatttcct ttttttgctg gcagatagtt
    48001 gctcaaaaat tgtttgaagt tcttttcacc aataaagcag attcccgttg aatctttttt
    48061 cttagcagtc gcaaggcctg cgcgttcagc aattgcccgt acttctgatt tttccatgcc
    48121 gcctagaggg aacatcgttt ttgaaagctg ttcttgtgat aattggctaa ggaagtaagt
    48181 ttgatcttta tcattgtcta ctccacgaag catgtggacc acgccgtttt catcgcgagt
    48241 gacttgcgca taatgtccag tagctacata atcggcgcca agatccattg cataatccaa
    48301 gaatgctttg aatttgattt ctttgttgca catcacgtct gggttaggtg tacgtcctgc
    48361 gcggtattcg gctaggaaat attcaaatac acgatcccaa tattcttttt caaaattcac
    48421 agagtaataa ggaatcccga tttgagcagc tactttcgct acatccttat aatcttccgt
    48481 ggccgtgcat acaccgtttt cgtccgtgtc atcccaattt ttcatgaata ttccgatcac
    48541 gtcgtatcct tgttccttta acaaaagtgc tgttacagat gagtcgacac cgccgctcat
    48601 ccctacgaca acgcgtgttt tgctgttgtc tgtcatgata tcaccatcat ttcaattaga
    48661 ttctgaaaca tctacgagtt gaaggtcgca acttttcagt attttacatt atacgtcata
    48721 tttctgaaaa tttcatccct taattatcaa gtcgtaaaaa tactgttcta ctggacctat
    48781 tcagtacaca aacatgatag tcaaccaaaa aatcactcat cttttgagca ttcgatataa
    48841 aaaattggca tactcataaa aagggccagc ctttcggcaa taaggaataa ttttataaat
    48901 tgtgaaaaac tatttttcaa aattgcttct tatcgcttaa agctgcacgt ctcatttaca
    48961 accttttctt tttagatgtt atgagttgct cagataagtg attttgttta ctctaagaaa
    49021 aaagggaggc aaaagataca tgaattactt tatcagtgat atgcactttt tccatgaacg
    49081 cttattagga aaaaacgact ttgctccgcg gctctttgca aatgtggatg aaatgaacca
    49141 acagttgatc actagctgga atgcagtaat aaaagaaacc gaccatgttt atcatctagg
    49201 cgaccttgct atgcatccgc aatatgaaaa agcaaatgca gaaattttgg aactggtcac
    49261 tcaactgcaa ggaacaatcc acttgatcaa agggaaccat gacagtcgcg cttttttccg
    49321 ttatttagaa aaaaatgatc ctaggcttac tgacggaagt cccaaatttt atttttatga
    49381 tgtaggtgcc atcgttaaat tcgaccatca acaatattac ttgactcatt atccgatgtt
    49441 gctcgggcct aacgacacta tccgcaatct tcatggacat atccatcatt acagcgtacc
    49501 tatcgagaac gatgtcaatg taggcgtaga cgcaccggaa agagaactct taaaggaaaa
    49561 actaccattt ggcactccgt tatctgagca acaaatcaaa gaaatctatg aagcaaagag
    49621 gaagtacgat aggtcgtgaa aaaactgccc tatatcaagt attaaacaaa taaagataat
    49681 tactcatatt tttcatttga gtaggcttat tgccacagga acattaggat cagaaatgga
    49741 gttgtaacaa gacttctgtc acaactccat tccatactat tcgtcttttt gctcatggtg
    49801 ttcttttacc agttttttct gtaattcttt atcataattt cgggcagctg acgttaaaaa
    49861 taaaacgatt ccgcataata aaatcccgat tccggaaaac aagaacattt tttctacccc
    49921 taaacgatca gctaatggtc ctgcaaataa taatccaatt ggacctgcca ggttcatcaa
    49981 agagttgaag ttccctaaga cacgacctaa aacattcgat tcataactct gctggatcat
    50041 tgccatcagc agtgtattga aatatggcgt tgcacaacct gctcctgcat tcaagataag
    50101 aaagtacaag aacccttgac tatcgtttgg aaccaatccg cttgcaccga tcgtcaggcc
    50161 cataatgatg taggctataa taataggttt cattcggtct ttccatgttc ctataaaact
    50221 gatcagtgca ccgcctaaga gcattccgac tgcgtagact acttccactg ctcccgcctg
    50281 tccaacggaa ccaccaaagt aatctagcgt catcaatgga tacagactga ttgctggcat
    50341 gaacaacagc atcacaaatg caccattgat cgttatgtac cataaccctt tattatccat
    50401 caattgctgc agcccaaatt ttgcatcttt aaaaatagta agcggaactt ccaatatttc
    50461 aggagaaact tttgggatct tcacaaaaat caataagccg acaccaaaaa ctgcccccaa
    50521 tacatctaat aaaataaggt aattgacagg aacgactgaa aacaaagccg cacctagagc
    50581 tggcgcaatg atgaaattcg ctgattggac cattcctaac tgtccattcg tccttgttat
    50641 gtgagaagat ggaacgattg tcggcataat cgactggatc gttggcattt ggaatgtttg
    50701 tgcgaccgaa cgcataaaca aagacacaaa aactagccaa atcgggaagg attctgaaat
    50761 cgttccgact actgctaaaa taagcgcgaa aatcgctaca ataatatccg tgacaatcaa
    50821 aagagctttt ttatcccagc gatccactag cggtcctaca aatgggctta gtaaaaccat
    50881 gggaatcatt cctagtaaag tagcaaaact caaaattgtc gccgaaccgg tttctcttgt
    50941 caaataccag atgatcgcat attggacgac catactagtg attcctgaca aaaactgtcc
    51001 cgataaaaag aacaaataat ttctcttcca attcgttaaa acgaccgact gttcattttc
    51061 cataaacatc cctcttttct ttttcatttt gtatagcgtt tgcacaacaa tcattatacg
    51121 ggattctttt ttcaaaaaat acctttttcc ctaaataaca atcgtttcaa tttaaatttc
    51181 tttacttaga aacaataaat cagcaaaata ttaaaaataa caacatgtgt taaatttaaa
    51241 aataaaaaag acaactaatt tgcgtcgtcc ttacaaagtt agtcatctaa caataggccg
    51301 tttcaaaact agttgtcttt ttatatccac agatcattcg actttttcga ataacccacg
    51361 gctaatcatc ccatccatca gaaaataaaa tttatcttgg ctcattttct gttttggatc
    51421 tttgaaaata ttgactgctt tcaatccatt tgctgtagtg atcgacattt tcaatgtttg
    51481 aatatctttt gatacagtga tttttaattt gaatccttct ttgctttgcg gcgtggcatc
    51541 taatggtcgg atcaattgat aattgcctcc gtttgtttct gtaaatccat tatcgcgcaa
    51601 tgtatatctt tttgcatcag gatgcaaacg atagctaatt ggtgcgccca gtaccgttgc
    51661 tgttgtttca aatgccatga ttttcacctc gataaaatta gtttctcttt attataaaag
    51721 tttatcctga tttttactag gtttttcagt taagttccct aaaaaatgaa ggcgttctct
    51781 cagacatctg ttcatataag aatgacttca ctagttattt tttgacagca cgaactaatt
    51841 gatcaaggaa atactggatg tcgtcttcgg tatttcccaa accaaagctg atacggatgg
    51901 attcttttag tatcggtgta tcttctccat acattgcttc taatacgtgt gaaggctcga
    51961 tatttcctgc tgtacaagct gaaccagtag aaatagcgac cccattaaga tcaagcttca
    52021 tcagtagcaa atcatttgct tcgccaatta agcgaagatt caacacatga gggagtttat
    52081 tcgttaaatc accatttact tgataaggga tttcgtttgc ttctaatgta tcaagaatca
    52141 attgctggaa tcctttatat ttgttgttac gtgcttcacg ttcttcaggt gacagcagtt
    52201 caactgcttt tgccataccc cagatccctg ctaaattttc tgtacctgca cgccgctttt
    52261 cttcctgctc cccgcctaaa agcaatgcag ggatatttac tccgtctcgt ttatataaga
    52321 aaccgacgcc ttttggccca ttgattttat gagcagagat acttaataaa tcgattccta
    52381 attcttctgg atggatcgtt tggtttccgt acgcttgtac cgcatctgta tggaaataag
    52441 ctggatgatc cgctaaaagt tcaccgattt ctttgattgg catcaaattt ccgatttcat
    52501 tgttcccgta catcacagag accaagatcg tatcttctcg taacgctttt ttgacctctt
    52561 cgatcgacag attccctttt tcatctacag gcagatatgt aacgtcaaac ccctttgtct
    52621 ctaaatattt catggtatta agaacagcag gatgttcgat agctgtagtg attagatgtc
    52681 tgccttcttt ttgacgcgag aacgctgttt ctaagatagc tgtattatcc ccttctgttc
    52741 ccccactgtt aaagatgatc tcgtgaggtt ttgcttgcag actgtctgca atgatttgtc
    52801 ttgctgtttc caatttttca tgcgcgtggc gaccaaatcc atgtatgctt gaaggatttc
    52861 caaacgtgtc ttgcatgatt gccgtcatcg cttcaattac ttgtggatgc atgggggtag
    52921 ttgccgcatg atccagataa atagttttca cttcattcat cccttccaaa ttattgctag
    52981 gcacattcta gcataaaata gcgaacaaac caaagaagag ctcatttttg tctagaggac
    53041 gtttcatggg catagaaaaa aggagctgtg acaaaagtct tgtcgcagct ccttgaagct
    53101 gctcacttct gtcacaactt ctttccctct tttttttgtt tattcgaatt ctttttcttc
    53161 cagacggaat aacggactca tcggtgtatt ttcatggatg cgtttgaccg cttcacccat
    53221 taattctgaa catgtaacga ttgttaagtt ttctggatga cgattttctt ctgtgaaaac
    53281 agaatcagtg atacaaatat ctttgattgg tgcattgtct aaaatacttt tcgcatgatc
    53341 ggacaataat ccatgtgacg cacaggcata tacttctttt gcgccgtttt cttttaatac
    53401 atttgctgct cgtgcaagcg ttgctcctgt gttcaagata tcatctacca tgatacatgt
    53461 ctttccttga acatcaccga tcacataacc accctcagtt gaagcatcat ctgcatgatc
    53521 tacgatggct aatgtgctgt tcaaatattc tgaaagacta cgtgcacgct gaacaccgct
    53581 gttttttggc gaaacaacta cgatgtcatc tcccgtcaga cctaattgac ggtaataatg
    53641 agcaaacaac ggcatcgtaa ataaattatc tacaggaata tcaaagaatc cttgtacttg
    53701 aaccgtgtgc agatctaaag tcagtactcg agttgcccct gcttcttcga tcaagttagc
    53761 gatcaatttc gcagtgatcg gttcatgagg tttcgccgtg cggtcttgcc tagcataccc
    53821 atagtaaggc aatacgacat tgatcgtttt cgcacttgct cgcttcattg catcgatcat
    53881 gatcagtagt tccatatagt aatcattgac aggtgcattc gttgattgaa cgatatacac
    53941 atgatcccct cgaatacttt cttcgatatt gatggaaatc tcgccatcac taaattgctt
    54001 cacgacgctt ttacctaatt ctgttccgaa cactttcgcg attttttcag ctaatggacg
    54061 gttgccattc aaactaaaaa tcttcaacgt gtcatcctga tacttattac tcatgtagtc
    54121 ctccccaaaa cttctctatt cttcgatagt ttaacatatt tttccaagat tttctgttaa
    54181 tttcagcaaa ttcttaagga agtgaaactg ttccgttact ccaactggca ggaatgactt
    54241 tatgataatc gccccagttg tcgtgtactt tgtaataacg ttctcctgtt tcagtttcaa
    54301 aataagcgta cgctgtcacc caatgattgc catatgtact gccaagtact ttcaaaatac
    54361 caatcatcac aggtttgcct tcacgtatac gttttgtagc tcgctgccat gaacctagaa
    54421 gagttgctcg tgcacgctca tgattgccat tcttctttaa aaaactagaa atccctgtac
    54481 taacctgaaa aggaatcgta ggaagtccta gtggttggat ctggctacgc aatgccttcg
    54541 tcaaaaccaa cgaatcagcc gtatttttct tgcgaatctc atttggaagc atccacccct
    54601 tacgatagtc ttgataatag gcaaggagaa ctgctgccgc gtaagtacca caaagatagc
    54661 ctgagggggt agcccatttc ttatacctca aaaaacgttc ttctggcaat cccacccagc
    54721 aatttgcgag ttccttttct gacctttttt cttcgatcaa aacgggaata tcttcttttc
    54781 tgttgatcca tctagataac tcgattggct cttcattgat cagcttccat tttttacaag
    54841 aaagaccctc ttgataagta tattttgctg gagaatattc taccggatgt cctgattgat
    54901 cgataatcac ataactttga tcttcaaaaa ccagagctaa aaaaagcaat ttactttttc
    54961 ttgactgcag ctgctgagtg ctggctaatt ttttaatttt tttatctact ttgaaatata
    55021 aaggtttatc cactacttga ttcagttggg atcgcctcct ttttttcatt ataagtgaag
    55081 gattgaaaaa aacaaatcag tttgattgtt cgaattttca attttttatg ttctatttgt
    55141 taattccttc attttgtaag ataatgggca cacgaaacaa aaaaggagcg atcgatgaat
    55201 gaacaaagaa cgattgaaac aagatttcta tgaagcagtc aatgaagaat ggcaaaaaac
    55261 agcaaaaatc cctgcagata aaccagctac aggcggtttc caagatttag ttgacgggat
    55321 cgaccaactt ttgatgggag aaacagacga tctgcttgcc catccagaaa aagtgtcttc
    55381 agaaaaaatg aaacatttcc ttgcatatta tcaactggca aacgattatg accgaagaaa
    55441 tgagttgcat gctcaaccgc ttcaacctat aatcgaaaaa gttgaatcat tgcagtcttt
    55501 cgacgaattg aataagcaat ttccagaatg gctgttggat acattaccat tgccatttaa
    55561 tctggatgta gatgcagata tgaagaatgc gcaaacgaac gccttctttg cttatccgcc
    55621 ttctttattc cttccagata agacgtatta tgcatcagac catcccaacg gtgcacaact
    55681 aataggcgtt ttctttgata tggctgttca gttattagag cttgccggca aagaaaaaag
    55741 tcaggcagaa gcaatcacgg aacaagcagt gcaatttgat aaattgattg ctcctcatgt
    55801 aaaaagtgca gaagaaaacg cagattacag caaaatgtat aatccgcaat cttttgatac
    55861 atttattaaa tacacagata aactggactt gaaaaaactt gtcacgggtt tgattgggac
    55921 aactccagat aaagtgatcg taactgatcc agtgtacttc gatcatttga atgacatcct
    55981 gacagaagct cattttccgt tattcaaaag ctggatgatc attaaaacag tacgttctct
    56041 tagtggttat ctatcagaag aattccgcca agtcagcgga atctattctc gcgtattgtc
    56101 tggaacagac gaagcaatgc ctcagaaaaa agcagcgtat taccttgcct ctggacaatt
    56161 cgatcaagta gttggcgatt actacggaaa aaaatatttt ggcgaagctg ctaaaaaaga
    56221 cgtagaacaa atggtgaaaa aaatgatcgc cgtttataaa aaacgtctag aagaaaatga
    56281 ctggcttggg gatgcaacaa aagaaaaagc cgtcataaaa ttaaataact taggtatcca
    56341 agtaggctac cccgataaga tcccagcatt attcgaccag ttcaaaacag tacctgcaga
    56401 tcagggtggt actttgcttt caaatactct tgttttcagc aaactcacat taaaagatcg
    56461 tttcagcaaa tggaacaaac cagtggatcg ttcagaatgg gaaatgagtg ctgacacggt
    56521 caatgcttat tatcatccat ttcggaatgt gatcgtcttt ccggcagcga tccttcaagc
    56581 accattttac agtctagaac aatcaagctc tgctaattat ggtggaatcg gagctgttat
    56641 cgctcatgag atttcccatg catttgataa taacggcgct ttatttgacg aatatggcaa
    56701 cttgaacaac tggtggacag aagaagatct tacacaattt caaaacaaag cacaagcaat
    56761 gatcgaagaa ttcgatggtc tgccttttgc tgacggaaaa gtaaacggaa aattaaccgt
    56821 ttcggaaaat attgctgatg caggcggtct aagctgtgca ttggaagcag caaaaacgga
    56881 atcagatctt tctatcgagg aattctttgt caattgggca acgatttggc gtacaaaagc
    56941 aaaaaaagaa taccaacaac ttttactgca aatcgatgtc catgcccctg ctaaactccg
    57001 agcaaacgtg caaccaaaaa accttgacga attctatgaa acatttggta tcacggaaca
    57061 agacgctatg tatctagcgc cagaaaaaag agtgcgaatc tggtaagagg ttgtgccaga
    57121 agtggtcagc ttcaagaaat aagaaggaat ttgggaaaat cgcttctcaa atttttgtga
    57181 catttcggct tatttttcga agaagttact tctggaatac catgtattag aggttgtgaa
    57241 aaagctgctt agtttcaagt agtaagaaaa attttgtgaa aatggctttc cacatttttg
    57301 cataaatttg acttactacc gagaaactgg cttttgaaca ccgtttatcg agaggttgtg
    57361 aaatcagcgt cctgacctga gtattagaga aaaaaacgga aaaatagttc ctcatatttt
    57421 gccgtttgtt gagctaatac cgagggtcag ctgattgaac accgtttagt ggagagattg
    57481 tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaagcagc aaggagaacc
    57541 tcatctataa aaagatagaa ggaagtgaga catctattgg tctcatttcc ttctattttt
    57601 actttcctca gtcaattgtt ttacatcata aaaggagaaa ttccacttat ttttttcgct
    57661 gtttcatgac ataaacaact acaccgatta acaaaattcc tccagctaat gctagcaacg
    57721 ggttattaaa ttctcccgtt tttgggagtc cttgagtgcc atttttttca ctttcttttg
    57781 ctgtcctatc cggatgagaa ggttttccat ttggttttcc atttggtgat tcatttcctg
    57841 gattttctct ttctggtttg tcatttcccg ggggcacatc ggcagtttct cgcacagtga
    57901 tcgctttttg ctgggtaaca gattctgtat gagcagacca tttttcccca tcaaatattt
    57961 ccgaacgata agtgacttct agcgtatatt ctccttttga aggtgctgaa aaaatagcgg
    58021 tatgattaga aaattgagca cttgactgtg tttcaacgat ttggatcttt tctgggaaat
    58081 aacgcatatc ttcaaagaca ggcgttcctt gatttggtgt gaaagttgac acaaaaactg
    58141 taaaaggctt cagttcaatg atatttcctt ctgcattctc cgccacacca tttgcttctt
    58201 gtataataat cggattattt tgttcatttg gttttgtctg atttggttct tgtacgattg
    58261 catactctgg atattccgaa gacggtgagg tttctccgtc aaaaatagca cctacagata
    58321 atggaagacc atccactctt tccttttctg tttgacgatt ccccggttta atctgcatca
    58381 gtccatgaac atggatactt ccattttcat agcgcgtaac aggctgctga ccttctttcg
    58441 aacatgtatt tagagactcg atccaatctt ttgatatggt atgtccaaga gtattttctg
    58501 attgtttctc tcgttcattc caatagtagt tcaacgagtg gtagatttga tcttcgtttt
    58561 gattcaatgg gcggatctgc tctctcatct caagattctc tgttcggaaa gataagatac
    58621 cagaagcatg gtagtctgtg tttcctgctg aagaggtata aagagccaca ttatatgaac
    58681 cgttattaaa acttgtactg ctattgacga taatatctgt tccgctattg gaatcgattc
    58741 ctttcgcaag attttcgaaa gacaaactat tgatcaattc atgagggccc gttaaagaag
    58801 aaccgcccat tttgaatcca ttaccgttac cttctccctc aattcctggt acccagccgt
    58861 ttcgataagc aactgagttt ttgatgatta cttttccgat cgaacctgtt tcatttttag
    58921 caaaaaggtc ccaaccgtca tctgcattat gatacgcgat acaaccgtca aatacatttc
    58981 cttctcctat tgtcagtttg gccgcaaatc catcggcatc ttcaaatcct ggatcggcat
    59041 tttcaaaaga tgtacagttc ttgactaagt tatgcgcggg ccatgcttcg aaaggatcat
    59101 ttgagctacc gctgatttgg attcctgtat ttccattccg ataagcatga atctcttcga
    59161 tcaaattatg atttccagaa atctgtaatc ctttttgcat agctgcacta tttgtaatgt
    59221 caaaccctcg taaaccccag tagtgactga acagtgtcat cccatttcca gttcctttaa
    59281 aatcaagtac aggacgcttg ttttctggat ttttctctgc aatcaactga atcgggtttt
    59341 ctcttgtccc gttaacgcct tttggaatcg tcaatcctct ttcgaaggaa taagttccag
    59401 gcgccatgac gattgtctgc ccagcatatg cgtatctcaa tgcttgtgtc agagataatg
    59461 gattttgtct gcttccgttt cctgcatctg acccttctgg tgttacataa actgtcgaat
    59521 atttaggttt acggtagtcc actgtatgaa gaatctcttt tgtttcataa ctagtcatct
    59581 ctacgtattc ttctggcgga tagtttttat ctggcgtaaa actgaaagta aattcgttcg
    59641 ttccttccat cagttttgtc tgataatcaa attcccgatt cgcagtgata gccaagtttt
    59701 ttgcgttctt aagcacctct cctgttgctt tatttttaat tgtcaataat ccgtcagcat
    59761 ttgctctaaa ggacatagta tgatcttggt ttcctgaatg attactagag gtcacagtgt
    59821 aaatcggatc aatcaagcga gtaggtcttt cttgcccagg aggatctgtc gcaggatctg
    59881 agtaagcaac ttcgattcct tctaccttga tcgtcatatt ccttgctgca gcaaatcctg
    59941 cataaataac cgattcatct gaagcgtata atttttccca gtcatacatg atgtcagaag
    60001 ccatttcttc tcctgtttca ggatcgtaat aatatgcctc taaaccagta ttcgtttttt
    60061 tcatctctac gtccagttta gtaatcgagt tcgtggccgg aaacagattc ccatgaccgt
    60121 ttccgaatat attataagaa cctgtttctt tttccaagaa ccctgctgaa gtctctaaag
    60181 gtgttgtttc cactgagaca ctcccaggag ctggcgcagc ttgcggatct tcagatgata
    60241 ttcctgtgat tgcccgtgtc cccaatccaa gtcgcatcgt atatttgctg ccgctcgtgt
    60301 tgaccacttc ttgtctatcc gagtcccaga catattcgat ccgactgcct agtatggcaa
    60361 atgaatttga ataaaatgcc tgcccaaaca aatgattttt tggtactgcg tctcttacca
    60421 ttaaagcaaa tccttcttgg gcattcgtat aggtccaaga ttctatttcg atcgtcgccc
    60481 gcatcctaaa gttgatgtct ttggggattg cttgataata ataggtcatt ccatctgatc
    60541 cgcttgactg gaattttccg ccgttattcg tggaagagag ccgcacgccg tcttctaatg
    60601 gttcaatggc atacctacta gcacttgtgc tttctccgaa aacaacccct ttccactctt
    60661 catttgcttc ttcagattgc ttttttgtct tttcattttc tgattctttc atcataggat
    60721 catttccttc ttccgtatcc atatcttcac tagttcttgg tatttcctcc attgttgaca
    60781 tcccttcaat gttcacgtgt gcattttctt cgttcacaga ctgttgttcg ttagcagcaa
    60841 cttcaacgac agaaattaaa accggggtac tgagcataag gagattcatc attagcgcac
    60901 tgtattttat acgttttttt aacaagtgta ttcctcccgt aacaaattag aaagcgttta
    60961 cttaaagtat tacctaattt atttttactg tcaataaaaa acaaaattat aataagtgta
    61021 ttttataaaa agaaaaaata taacttaagt tgttgataat aaagagattt attaacgtaa
    61081 aaagaataag tttttttaaa ataacgtata aaaaagagca taaaacaaag tcgtttgttt
    61141 tatgctcttc ttttattatt ttgtgctttt tattttatca aatcactttg tttttttata
    61201 aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc ccattcatgt ccaacgggat
    61261 cgaaacttgc cacggcatct ttgaaaatat ggagtttgta tcctagatta tacgcatcga
    61321 ctgctgtatg caacacacag atatctgtac atacgccggt caaataaata tctgtgatct
    61381 ggcgttctcg caaacggatg tctagatctg ttccactaaa agcggaataa tgccgtttgt
    61441 ctatccaata aacattttcc tcttgctcat agtcttggta taaacttttt aaagagccgt
    61501 acaattcccg tccacccgtt cctatcacat tatgcggagg aaataagcgg ttctccggat
    61561 gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac gacaaaatcc ttttgatcaa
    61621 taaacttctt tgtatgagca gtcagctttt tttcaattac ttgtcctgct gtcccagtag
    61681 tcagctttcc atcctctgca acaaaatcat atgtgtagtc aatcgagatc aatgcgttca
    61741 tcttagtcct ccttatacga gatgtttgac aaattcgatc accatttctg ctgatcgttt
    61801 gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc gagtgatctg ctgtgtcgct
    61861 cattgcacgt acgatcaaaa atgggatatt gaactgacga gctgtttgtc caacagcggc
    61921 tccttccatt tcgcaagcta atgcttcagg gaaattcgtt aagatttctt tgattttatc
    61981 tggcgagtcg acaaatgtat cacctgtaac gatcaatcct tcttttgcgt tcaaattcgt
    62041 tgcttttgct gctttaacca tttcactgcg caaatatgtg cttgcttcat aatatagagg
    62101 cattcctggt aattgtcctg ggttgtagcc aaatcctgtt gcatctgcat caaaataagc
    62161 tactttgtct gatataacga tgtcacctac ttgtaagcct tcaccgattc cgccagctga
    62221 acctgtattg atcaccatgt tcacgccgta ttgttggatc aacagacttg tggtaataga
    62281 tgctaagact tttcctatac cggaacgtac gacgatcact tcgtggttac ctaatgaacc
    62341 tgaaataaat aaagcgcctg cgcgttccca tgataatggt tcgcttaggt tctctctcaa
    62401 aatttttact tcttcttcca ttgcgccgat aataccgatt ttcatctttt taattccttc
    62461 tttctatata aatgcgattg ctaaaaaaac cacacataac attaggataa caataataag
    62521 agatttcatc aaaaaagagt tccatttggt cgtcttttcc cgttcgctga tccttgtttt
    62581 cgtgattggt ttattctttt tgctttcttt acgataaaag ctggcaatct ttttttcttc
    62641 ttgctgataa gctgtttctg cttttcgctg tttttttaat gactcgcttg cttgtgcttg
    62701 ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct ttggccataa gccgatccct
    62761 ctactttcct ttggtcaata gttcccagta ttgaatcgca taaatcgtct ttgcatcaca
    62821 aatcaactga tctttcatcg cttgttgcgc ctcttcaaga gttagatgat acaactccag
    62881 tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc tctacccctt gtgcatgata
    62941 aatatgaagg acttcatttg caaaacctgg tgaaagatac atcgaagtaa gatggctcag
    63001 cgacttcgca cgatagccag tttcttcttc taattctcta gctgctgtca tttcgggatt
    63061 ttgtccttct cctggatcga tttttccagc tggaatctcc aaaatcactt tttctaaagg
    63121 ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg tcaaaagcaa tgatcccgac
    63181 accgccagga tgaaatacca gttctctctt cgaagttcct ccatctggca aacgaacttc
    63241 gtctacagca acatcaataa tttttccgct atagatttct ttgcgactga ttgttttttc
    63301 ttcaaattgc tcattgttca gcatatcttc tcctgctttc tttgtaatca taactgcttt
    63361 tttttcattt ttcaattttt ctcaaaaact ccttattagt ttaccataga taaaccttag
    63421 attttctcta aatatagaaa atccttcttt agtccgatac tctttatagt agtccgtatg
    63481 ctaaaataag tatgtaatcg aataacgaaa agaattcaat actcaacgat aaatgacatg
    63541 gagggtttat tatgataaaa cgaaattggc cgtggcttct agcattggtc ttgctatttc
    63601 tctttgtccg aaacaatagt ttttcttttg ttctaggact tttattgata ggtgtagttt
    63661 tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg aaaacaagaa gaaattcctg
    63721 cactgactga cgaacttgaa actcactatg aaaaaagcgg tatgacagcg aatgaaattt
    63781 cattttttcg acaaacaatg aatcaaacaa aaaatgagat caatcaattg caaatgaata
    63841 tgcagcagac agccaaactt aaagcaatcg atctccgcca tgatccagtc aaagcagcga
    63901 aagctttatt caaagaattg gtgaaagaac caaaccgact gcatgaagca agtcagtttc
    63961 tttatacgca tctgccgaat ctagttgatt tgacaaataa gtataatgag atcaatgacc
    64021 atgagatcaa aaacaaacag acctatgaaa aactagaaga aagcgcgcag atcatcgacc
    64081 agctgtcagc tttaatcgca caagattatc aaaaattcgt tgctgaggat ctagatgata
    64141 tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg tgataataaa tatgaaccag
    64201 aacaaaagga ttaactcttt tgttcataaa agaagactcg gaacattcag atgatgtctg
    64261 gctgtttcga gtactttttc taaagtagag acattcatgt aagaatcgag aacagattca
    64321 acaaggagga attttttatg gatcataaac cgactgaaac aaaagatgtt acaccagtta
    64381 acgatacact tgaagattta ttgaacaacc cgttttcaac tccaacagat acgctgacca
    64441 cttcacaaca agcggagatt gatcagctcc aagagcaaca gacagctgct cgtttaattg
    64501 acaaactacc ggcggaacgc caagaacaag ctaaacagct agcagcaaaa atcgatgcta
    64561 atgatgcaca gtctgtcatc agctacggat cggctgccca agcaaaatta agtgaattct
    64621 cacaatcgat gttgaatcat gtgcaagcac aagatattgg tcctgtagga gattccttaa
    64681 ctgaactgat gtatcgtctc caagaagcca atcctgacga actgcgtgcc ggagaaggga
    64741 acatcttcca gcgtgtcttc gggaaggtca aacaatcgat ctatgaagtg acagcaaaat
    64801 atcaaaagat tggtgcacaa attgacaaga tttcagtgaa attagataaa gaaaaagacg
    64861 gtctgctaaa agataatctg atgttagaac agctttacca gaaaaacaaa gactactttg
    64921 atgcactgaa tatttatatc gctgctggag aattgaaaat ggaagaactt caaacaacga
    64981 tcattccaga agcgatgaaa agagcagaag aaactggcga tcagatggat gttcagattg
    65041 ccaatgatta cacgcaattc ctagatcgtc tggataaacg tacgcatgat cttcgcttag
    65101 ctagacagat cacgatccag caagcaccac aaatccgttt gatccaaaac acaaatcagg
    65161 ccttggctga aaagatccaa gcatctatcg ctacagccat tccgctgtgg aaaaaccaag
    65221 tcgtgatcgc attgaccttg cttcgtcaaa aagatgcagt aactgctcaa cgccaagtct
    65281 ctgaaacaac aaacgactta ttgaagaaaa attcagaaat gctgaaaatc tcagccatcg
    65341 aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga gacccttcaa accactcaaa
    65401 atgatttgat cgaaacaatc caagagacat tgcgtatcca aaaagaagga aaagaaaaac
    65461 gtcgtcatgc agaagtagag cttggacaca tggaagaaga tctgaaacaa aaactgttgg
    65521 atttaaccca atagatcacc tttcgcttgt tactaaaaca aacgcccaac aacagtttat
    65581 tacaactgtt gatgggcgtt tttcttatct ttcttctaac ttctccattg gagtctattt
    65641 ctcattcatc ttgctgtgtc catagccata gccaaaataa atgatcagtc caataaccat
    65701 tacgataccg aacacgatcc atgtcacagt ttgcagacga agcatcaata caatacaagt
    65761 caagatggag acgattggca atagcggaac gaatggcact ttgaattctc cagcttttgg
    65821 tttgccaaac atttttctca acttcagtaa gcctagacta acaagcatca aataggcgag
    65881 cgccatgata ttcgttagtt ccgctaatac attcaatggg aaaaatccgg caaagaatac
    65941 agctgccaca ccagcggcat aagtcgcttt tttcggtgta cgatgctttt gatcgatttc
    66001 tgtcataaac ttaggaagta agccatcctt gctgacagca aacagtaaac gagcaagtga
    66061 atacatcatc gagatcgtta ccgtcagtag cgttaaaatt gcccctacag cgatcaccgc
    66121 gcccactgtt ccatgtccta catacctcat tgcaaaagcc actgggtcat tgactcctag
    66181 ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg atgatgtaaa ggacagtagc
    66241 aatcagtatc gaaccaatga ttcctcttgg cacatctttt tgcgggtttt tgacttcctc
    66301 ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa aacacaagag ctgcaccatc
    66361 tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag ttatcaggtt taacgaaaaa
    66421 tattcctaca atgataaaca acgcgatgat accaaatttg acatatacca tggcattatt
    66481 caaacgtaat gctttcttgg cttcaagcga cacgatgtaa gtgacgaata tcactacgag
    66541 caccgcaatc aagtcaatgt atgtaccatt ttccggattg tacccgccag ttaatgcttg
    66601 tggaagttcg gcccctaaac ttttcaaaaa accttgcata tagccagacc atccggaagc
    66661 aacagaggaa accgctagta aaaattcccc gatcaacaac caccctgtca gccaggcagc
    66721 aaattcacca aatacaacat acagataggc atatggccca ccaatcactg gcactcgcga
    66781 agcaaattct gcgaaactta gtccagacaa aatgacaacg attgcagcta agacaaatga
    66841 aagtgacagt gctggaccag cattttgatt ggccgcaacc cctgtcacca caaatattcc
    66901 cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca gacgttttta gttctttttt
    66961 catctcactc gtatgattga gattgatctc tttcttacga aacaaatcca ttgaagttcc
    67021 tccccatttt aggttggaaa acagcgatta aactgttttc atatacccta aaaggatacc
    67081 acctaaaatg actaaaaggc aaccaacaat aacataaatg agctcttttt tcgtttttct
    67141 ttcgccaagg atgaaaatcc cacctaaagt agagataatg atccccattt gtgaaagtga
    67201 gaagccaaca gctaaaccaa gtgatttgac tgttagaagc atgcagatgt ttcctaagcc
    67261 ccataataat ccacagatca tgtttttcca cacaaactga tcgactttaa ctttacggaa
    67321 agcaaagaag cttgcgccta agatcattcc gatactttga ggtaagataa ttgacatcgc
    67381 atccaaattc gcccagttga cgatgatggt atatacacca taaccaatcg tagagaagat
    67441 caatgctcta aaacccttac caaaatcaag cattttatta tctgtatcta tttttccttc
    67501 gtcatcttgt cgggcagtca aatacgcacc gcttacaaga agagctaaag caataatacc
    67561 gtatacataa tctttcgttt gtgtccattc atggaagaaa acggctcccg caatcgtatt
    67621 caaaattaat tgaaaaccag tagacaatgg tagccctaca gaaacaccca tgtacttcat
    67681 tccatggaac tgtccatttt gtccaacact ccaagctaag ccggataaaa atccgatcaa
    67741 gaaaatccaa ggggtgatta caggactaac aacgaaaaag acaactaaag aaaacagaaa
    67801 tgcaccgatc gtcattccta gtgtttgttg attggcatcg ccaccaattt taccacttac
    67861 taaaccaata ctaccccatg ccaacatagg gactaatgct attaaaattt ccataaccaa
    67921 tctcctttta tttcacatga taagaaactt atcagcgaaa acgttaacaa cacaagcact
    67981 ttttttcgtt tctaacgatt ttctaacgtt tttcattata aaaatttaag attgggaaaa
    68041 ataaagtcat tgttgtccct tttccttcag ttgattcagc cgtcaaatgg atacctaatt
    68101 tttcggccag attttttgct aaataaagtc ctaatcctgt tgaatgtgtt ttgctaagtc
    68161 gaccatttct cccagtaaat cccttatcaa agatccgttt caagtcttcc gctgggatac
    68221 cgatccctga atctttaact gaaaggctga ctccttgcgc ttccttttct atgctcacaa
    68281 tgatgtttcc gtgatccggc gtatatttga tggcattact aagcaattgg ttgaagataa
    68341 aagcgatcca tttagcatcg gtcaaaacag tatgatcctc accaacgatc tcgtagcgaa
    68401 tatttttttg gataaaataa tttgcctgac tgcgtatcac aggctgaatg attcctttta
    68461 gtccatattc ttggatgagg tagtcccgcg agaaactgtc gagtcttgca taatacaaaa
    68521 cttgttctac gtattcatcg atttttgata attcattttc tagtaatgta tacttttgat
    68581 cgtcaatatc gaactcgatc gaacgaacta gcaatttaga agcggaaagt ggcactttga
    68641 tttcatggac ccatgagtca atataatctt tttggtcttg ttgattactg attgctgatt
    68701 gcattacttc ttggtgttca cggatcagct gattgatata ttcttctacc agtttttctt
    68761 cttctgtcct cccaccactc aaataatttt gcaaagggga agttttttgt atgtcggcta
    68821 attttcccca ccatcttctc ttgctcaagt aaccacccag cagataaaaa atcagaaaga
    68881 cgccctcaat caaagctaaa taagcaattg tgctcaaatc aactttgatg tttggcgcca
    68941 gccacatcac aaagatcgtc aatgcaataa aaaacagcca tcccaagatc aatgtccact
    69001 gatctttcaa atatttaaaa aaattcatct taaccgctca tcctttcagg gaacgatata
    69061 tccctgtcct acttttgttt cgatatatcc ttggatacct gcctgttcga tttttcggcg
    69121 taaccgattg atgttgactg tcaatgtatt gtcatctaca aatcgttcgt catcccacag
    69181 tgctcgcagc aatttttccc gacttaagat ctttccattg tgcttcatca aaataaataa
    69241 tagacgatat tcatttttgc tcaaatcaat gatctctccg ttgatttcca tacttccgtt
    69301 gtcaacattc aacgtaatgc cattgtggct catcatttca ctagataatt ctgtgtagtt
    69361 gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt acatcgattt ggaaatgttt
    69421 tgtcacaaaa tcatctgccc ccatgttcat agccatgatc atatccatat tcgtattcct
    69481 gctggagata aaaatgatcg gtactttcga tacttcacgt attttttgac accaataata
    69541 gccatccaaa acgggaagat taatatctag taatacaagc tgtggttctt ctctttcaaa
    69601 gtctgagaac acttgattga aatcggtcgt tccaaaggtt tcaaattgcc atttctcaag
    69661 ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg actaccatga tttttgccat
    69721 aaaaaatccc tccatctttc ttcttgctac tacaatatca aagaagccct tgtgaaacaa
    69781 gttggcttac ctggaaaatg tcattttagc aaacgttaaa aaaaactgga aatatgcgat
    69841 ttaatcgcgt ttgtctcttc caagcagctt aaatttatta tgttattctt aataattggt
    69901 ctattatctg ttatttttct ttcgttgttt ttgtctttta aattttcgat aacaccaata
    69961 accgaaaagc gttagcacta gcaatatacc tagaaggagt ataagtaatc gataaagatg
    70021 gtattttttt gtctgttcta tctttgaaga gacttcttct gtaaagggaa ctcgtcttcc
    70081 tgtcactagt aatcggtgag tgttcacggt gtaaggtgta caagtgataa gtgtgactag
    70141 atcctgcttt tctctaattg ctaatgtatt tgtttttgtt ggaaggacaa caattttctc
    70201 aacgacttca taagcgagca tccgaccata cacttcaaga tagaatcggt catcttgctc
    70261 catttcttca agatccgtaa agagtttttt ctcagcaata ccgctatggg cggtaatcac
    70321 ggaatgtgta ctctctcctc cagttggaaa agaggtgcct tgcaggactg tcgctcctct
    70381 atctaacaaa agatcattcg tttcatcaaa taaaggcaag ctaacattga ttttcggaat
    70441 atagatggct ccgatcatat gttgttcgta atattctttt cctggatttt ggacatttct
    70501 aaccgcttgc tcaaaaggat ctttgacttg tcccattcct gggattactg atgtatgagc
    70561 aagcgtctga tttttctttt catgcactgc taattgttgc tcatgttttt cttctttatc
    70621 gcgttcaagc tgcttttgat aattctcgat ccttctttga tccaaataat tgctgagagc
    70681 atctgctaaa aatggatatg taaaaactgc agctccggca agaaacatca ctgccataaa
    70741 taattgtaat aaaagactga tttgggtttg aggtctatta cctattttct ttttcatttt
    70801 cttcaccttc ttatttaaaa aaactgcagc aacctttata aaaccgattg ctgcagctta
    70861 tttttgttgc ttatacttca gacttcattt tagatttacg gtaccagctg tacgctccaa
    70921 tcatcaaact tattccgatt agtaagaatg ccaagagccc gttcccacca gtagcaggaa
    70981 gtgacccgcg tttcatattc ggtactcttt ctggccctgg attttctact cctacgatct
    71041 gaatttcttc attatagctt ccataagaga tgataaattc agtaaaggct gcctcacctg
    71101 ttggaagtac ataaccttct ggcgccatgg tctctttcat ttgataggtt ccataatcta
    71161 acccttgaac ctcaaagtat ccatttccat ctgcgacaaa ttctgttgct tgtccgcttg
    71221 ttgcccactc aattctatct gctcctgatt cataaacagc taaaggcgac tcccctttat
    71281 aaaatattgc gtattcagaa cggtcttcat tccacaattc gaatcttgca tctttaagca
    71341 attcactgct tgatgcatca tatttttcaa actttcttcc acctgttgtg actttctctg
    71401 gcggctctac tccttcttca taatcaaatt catcatttcg tccgatttca ataaccgctg
    71461 tgttgttatg gaaatcatcc gcttctagtt ctgtggaaac cttcaaatta tacgtgatcg
    71521 ttaaatctcc tccacgtgca tctgctaatc tacctaaagt atctactttg gctactccat
    71581 tttcttctgt caaaatcgtc acgacaaacc cgttgcttac tacttctact atataatctt
    71641 gtccttttgt taaaatcgtc tcgccggctc gcacttctat ttcctgatca taataagtta
    71701 gaccaactgt aggcgtatct gtgattttgt aatttttgat aacagcgctt ccgttttcaa
    71761 ggacagaatc gatgaaataa ggaataggtg ctgtgatcgt gtaagaagcc gtttttcctc
    71821 tttctagatc aatataagaa atcgtttctt gttctccagc ttcattaata atcgttactt
    71881 gtctaaggtc gctttcctct acattcatct gtttatctgc ttctctgatt tcgtttttcg
    71941 gatacaaatg cacatctgta ttgtatgtgt ctttccatgt agaaccatcc cacacgccat
    72001 ccaccatatc aggcatcatc actggcatca taacaaccat tggagacgcg attctggaaa
    72061 tatgagctgg cgaataagtt tctaagaaaa gatagacttt atctctagaa gaaggtgctt
    72121 cttccgtcac ttggacggta gagaataccg cttctccagc ttcattcgtt gtttgtttgt
    72181 caatacttgt cagtcgatat tttaaaaacc aactttctgt gtgccgtttt tgaatccatt
    72241 caatcgcttc ggcagaatta agaccgtctt ctcgttccgg tgttaaagga ttatctcgaa
    72301 tcaaatcgta atatacttcc gttacgtcaa acaaatcaaa atcgactcct ggaatatttt
    72361 cttcaccaaa atccgtagaa accaatccgc tattcggtcg ttcttccgga acagatgaga
    72421 aacctttttt gtgtaatgta atcgtcactt cacttggttt ttgtgcgact tcttcggctt
    72481 tgactagttt tccgttcccg aaaatcccta ttcccgcagt aacgagtaac agtacactca
    72541 ttcccgtgac aatcattttt ttattcattt ttttctcccc gttctttttt tattaataaa
    72601 acagcaccta acaataaaag aattcctagt aaagaaatca acgttcttgc ttcattcgtt
    72661 tgcgggaata cggaccattt tcctgattga cctggattgc ttggaccact cggctctttc
    72721 ggtttgtccg ttgtttcttt tacggtctcg ttataaacac gtggtgttgc ggattcatag
    72781 gcagagactg gaactttccc ggattctaat tcagccatct cctgtccgtt aactactaca
    72841 tattgtggtt ggccttttgc atccacccaa aattcaggga tgatgacctc gattctctta
    72901 cttgcttcat ccatctcgta accttccaca ccttttaatt cttcaaaata ataggtgcca
    72961 gaaggcaaga aacgttgtcc tgtcgtaaca aggccctctt tatccgaaat gaatttgctt
    73021 acatttttgt cattcaaagg atcattgtct gctggtttga tccattgatt tttcaagtca
    73081 ttagcaggag ccatatctaa ataatatttc cccccttcta tcatctgata tagagcaaaa
    73141 acagcacctt ctaatggttt tcctttttca gttgttcctc tttgacgtcc atacttgaag
    73201 aagtatggat ctcttagata accgacattt ttaggataga tatgaatctc ttgcagttcc
    73261 tcggttgatt ctgtcgggtg ataaattggc aaaatagcag caattgggat agctgtctga
    73321 tcgatatcaa catccaatcc aacttctgga tcaatcgatt cttcgaaaat caaataaact
    73381 gcatcccgat cttggttttt cttaggtgct ttgatacgtg caatcccgtc tcctaatccg
    73441 ccaaccgtat ctttcccagt tgttaccgaa gtccctattt ggacaagcct ttcttctgtg
    73501 attagctgac ggatattgtt tctgttcgtt tcagcgattt gctggacgaa ttctttagga
    73561 gtcatgtttt cagcagcttt tttttcatga taataatcgg tcatgtcgta aatggcaaat
    73621 gtcgctccat ttagtggcac cgtctgtgaa atgatatctg ctgtttcccc tgcttcctca
    73681 gagatagcca agccatcatt ttcatggtat tcaaattgat cgttataatc gatatctcga
    73741 atcatccgtt tatgcaacac gaattccacg acatcttccc ctgattcttc tgccgacatc
    73801 gcatgtggaa gaaggactgc tgcaagcggt agtaaaacaa gtaattgaag catcaaacac
    73861 aacagctttt tcattacctt gctccctcct ttttagattg tctgacaaac aaaccatagg
    73921 ctgctcctgc aagcgccatg aagaatgcag caagcaggat atagaataat cgtccggatc
    73981 ctcctgtagc aggcaacgga ttttcaggat cgttcccgac actaagctgt aatctgattc
    74041 gatttgtctc caaaagttca tattctaact cttgctgtag atcaggcgtc tctgcttctt
    74101 cgtttggata atacgaaatc tcaactttta gtgtacccgc ttcttttcct ggaactaccc
    74161 gatcattttc tatgtcaggt tcttctcgat aactaatttc taaaataaag taacctggaa
    74221 gttttctata gctgtcaggt gacttcactt ccttgatccg atagacccct tccaacaagt
    74281 ctgaagcatt gaatagggaa gaatcgtttg gatctggtgc caagcctccc tcgatgattt
    74341 catactctcc tgcactattt tgtttctcaa ttgaaaattc tgcgccttca agacgtcggc
    74401 tattcatact gtcaattttc aaaagttcca aatcgattgg cgctagttca tcttctaagg
    74461 ttacttgtag tacaccatcg atgatttcat agccttccgg caagggagca gtttcgtctt
    74521 cgatgaccgt atcttcaatg ataaacttac cttcttcatt cagtgcaaac tggaacactt
    74581 gatcagatag ccgataccca tttggtgcga cagattccga gacctcatat tcaccaatag
    74641 acagatgcag aggttggtcg taatttaaat cgacatactt ttctactcct acggcacgag
    74701 aatagaagta tccgatccca ggcaaacgtt tcgattcttc tggttctaac ggattaccag
    74761 tgtatgctcg atacagcggg aaggaagaat cccccacttt cctgatatcg aaacctgcac
    74821 catctaatgt atggccttca tggtctaatt ttctaactcg gatctcccga taatcaaacg
    74881 tattgtaagc agtgaatgct acccataaac gatcctcttc atctgttgta tacgtgagat
    74941 attgccaatt ttcttcacct gtttgcttga gttggatagt ccctatagca ttatcgtaaa
    75001 gtcgcaagat ccatgtgtca ttttcatcag gttctttgaa accttctggg ttctggctct
    75061 caatgatttt gtattctgca tagttggtag tttcttcact atccttataa acaaatgaat
    75121 ctggctctcc gattggatga ataactttct caccatccac gattacatat ttcccgtctt
    75181 catctactga atagaacgtg agtctccctt tctcttcctc tacttcgttc acaccagaaa
    75241 gccctttcgc tactggatat ccgatttcca gtccgttacc atctgtttca taaagttcaa
    75301 actctgcgcc attgattggc atattactgc cttcaattag tttattgata ttcatgtgga
    75361 ttggcttgaa ttcatttcgc atttccagtt tgacatacaa ctcatcatac tctgtgcctt
    75421 ctatgttagg ctcttgtaca acagaaagct tgtgatattc tccttcataa taggaattcg
    75481 gatctttacc ttccggtccg ccgactctcg aaccagatgt atattcatct acaagctcgt
    75541 agtgacctaa catctcatct tcgttggctg catatttttt gatttggaat tgatacgtct
    75601 catctactaa ctgataacgt ggtggtccag cgatctctct gatccaaaat atgtcgctga
    75661 ctgctccagt ttcatctcct aaaatcggca tcttcgtttc aaagatcgct attccgtccg
    75721 tggtagtttt acttgtttcg attggttcgg cttccccact ctctggattt tggtcatata
    75781 atgcaaattc gactccagcc aacggctcgc cattttgacc cttcttgctg acttggaaat
    75841 tacggccttt atgttcgtaa cgaatggaag aagatgggaa atacatccgt ccagtagtga
    75901 tatcattctc ataatcctcc aggttctgaa tataggccat attgttcact gtgcaaaaat
    75961 ttccgtcttg agctcccgca tcaagtttta cttggtactt gatcctgacc atttcccctt
    76021 cacctaaaga aatcggagaa aatttataag cttggttttc ccgtccgaca tacgttgtcg
    76081 gagtcgccac tacttctggt ggggcttgtc catgacgttc ttcatcccat atttctggct
    76141 cttggtactt catgccgttt tctcctggat ctagatggta aaactgaata tctgccgatc
    76201 tgccagctcc aaatggtgag acattaaaac cagtttcatc ataaagtgtg gcttgctgga
    76261 tcgttctctt gaaggtagaa gagatatcct ccaaggctgt aacaatgtca ttcttgttgt
    76321 tcgcattgta atatcgcggt gttacgccat cttcttctaa atcagcaata tttttcaata
    76381 catttcgtcc aatagcatct agtcgttgtc tcccgctaac gtttccatcg attcctaaac
    76441 caatggagaa gatacttggc gggttctctt gatacttgtt tttcagccaa tgatgataag
    76501 caactgtgtt gatcgcagat gacttttctt gcgaagtagg actgccattc gtattgttat
    76561 tagaagaacc atcaccaacc cagtgagtcg ctttattatc tccttctttt acctctccat
    76621 aacgatagtg tacatttact tcatcttctg gacggttaaa gtcagatgca taggtaactg
    76681 gatgagtaaa ccctgcatta gaagtattcc cagaaaactc ctcaaaattt ctgaacaaaa
    76741 cgtcattatc ttcagcaatc ttatcttcca tcacagtcca gttgctccaa gctccattag
    76801 tgctcgtacg ataacttgac tgaatcggtg cataactgaa ggtcggtgtc ccatctccca
    76861 caacgattag gattttttct ggatttctat tttctccgcc attgtcttta tacaatgttt
    76921 catagccatt tttaagccct agtgtaagcg gagttcctcc gattggtgta cgatttaatg
    76981 ctgtattctc acttcccgtc aaacgattga tatcatttgt caatggaaag atgtccgaac
    77041 tgatttgctc atggttattg atagctgtac tattatggta aaaattcacc ataccgattc
    77101 gaatatccag actcgggtca gagagcagat ctttactcat ttcgtttact gcctcaatca
    77161 acgcagcatt tttagtttga ctattggtcc cagcggttaa ttcactcata ctcgctgact
    77221 tatctagaac aaaaacgata tcgattggac tgatttcctt tttctcgccc ccgatgacat
    77281 ctagatagac attgtaaagg gttgttgcat cttcaggatg tagatcagat gctgtgctgc
    77341 ttggcagaac aggttctacc cattttttga tgtacgcccc gttataatcc aagtaactgt
    77401 tttcaaaatt ccgttcattt ccccaaagct gaacggtttg agctggaacg tttctatctt
    77461 cttcgcctgc agttgatcca ttattagtag catagtcgta atttcgccag ttcgctcccc
    77521 cgtttgtata atcactatca tcgatcaagt tctctccgtc atatggcgag cctttgtctc
    77581 cttgatagcg ttcattgctt tgtgtccaat ttcctaacac ttttgtatag tcattcgttg
    77641 taaacttcat ctattctttc atcaaccacc acttgaagag gtagttaccc ttcgttcaca
    77701 tcggggatct tttctgtttc aaaaatcaat gtgccggttt ctgcttcttt tgaataaact
    77761 tttccgacat accattcttg accatcgatc gtctcgactt ttaggtcttc tccatcttca
    77821 tcataggagg atagacctgt tccacgaacg ttttttacgg taccaatccc ggcggctgcc
    77881 gcatcgattg ctaaacgtat tctaccttca ttggcacttt cctttttttc aaaagccaga
    77941 tgccattcaa gggtttcttc tttttcaata tacttccctt caatttttgc atagtcctca
    78001 tctaagaata cctcatcctc ctttgtttct gttgactcag cagctacatc cagggtcgtc
    78061 atatgataga ctgactggaa atatggtcca aatagcaaac tgacagttac taactgcaaa
    78121 agcttcaatt ttaacccttt cacatcctta tactcctttc aaaaatattt gtcttctttc
    78181 acaacaatca acaaaacaat gcccatctaa ttataattta atttaatttt aattttaaat
    78241 caaatgatat cctttttagt t
//
LOCUS       DACEQH010000005        71539 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.005, whole genome shotgun sequence.
ACCESSION   DACEQH010000005 DACEQH010000000
VERSION     DACEQH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.005"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(606..962)
                     /locus_tag="IW833_000322"
     CDS             complement(606..962)
                     /locus_tag="IW833_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ1306469.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /locus_tag="IW833_000323"
     CDS             complement(976..1386)
                     /locus_tag="IW833_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1306470.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1732..3918)
                     /locus_tag="IW833_000324"
     CDS             complement(1732..3918)
                     /locus_tag="IW833_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1306471.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4136..4819
                     /locus_tag="IW833_000325"
     CDS             4136..4819
                     /locus_tag="IW833_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ1306472.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4900..5619)
                     /locus_tag="IW833_000326"
     CDS             complement(4900..5619)
                     /locus_tag="IW833_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1306473.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(5931..6137)
                     /locus_tag="IW833_000327"
     CDS             complement(5931..6137)
                     /locus_tag="IW833_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306474.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6382..6984
                     /locus_tag="IW833_000328"
     CDS             6382..6984
                     /locus_tag="IW833_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ1306475.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7125..8406
                     /locus_tag="IW833_000329"
                     /pseudo
     CDS             7125..8406
                     /locus_tag="IW833_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
     gene            8419..9147
                     /locus_tag="IW833_000330"
     CDS             8419..9147
                     /locus_tag="IW833_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1306476.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(9216..10739)
                     /locus_tag="IW833_000331"
     CDS             complement(9216..10739)
                     /locus_tag="IW833_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1306477.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            10991..12172
                     /locus_tag="IW833_000332"
     CDS             10991..12172
                     /locus_tag="IW833_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1306478.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(12232..13860)
                     /gene="treC"
                     /locus_tag="IW833_000333"
     CDS             complement(12232..13860)
                     /gene="treC"
                     /locus_tag="IW833_000333"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ1306479.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(14311..17022)
                     /locus_tag="IW833_000334"
     CDS             complement(14311..17022)
                     /locus_tag="IW833_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1306480.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(17032..17733)
                     /locus_tag="IW833_000335"
     CDS             complement(17032..17733)
                     /locus_tag="IW833_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1306481.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            17919..18518
                     /locus_tag="IW833_000336"
     CDS             17919..18518
                     /locus_tag="IW833_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1306482.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            18599..19612
                     /locus_tag="IW833_000337"
     CDS             18599..19612
                     /locus_tag="IW833_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1306483.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            19720..20637
                     /gene="rbsK"
                     /locus_tag="IW833_000338"
     CDS             19720..20637
                     /gene="rbsK"
                     /locus_tag="IW833_000338"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ1306484.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            20665..21060
                     /gene="rbsD"
                     /locus_tag="IW833_000339"
     CDS             20665..21060
                     /gene="rbsD"
                     /locus_tag="IW833_000339"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ1306485.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            21060..22433
                     /locus_tag="IW833_000340"
     CDS             21060..22433
                     /locus_tag="IW833_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1306486.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            22790..24934
                     /locus_tag="IW833_000341"
     CDS             22790..24934
                     /locus_tag="IW833_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1306487.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(24986..26027)
                     /locus_tag="IW833_000342"
                     /pseudo
     CDS             complement(24986..26027)
                     /locus_tag="IW833_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(26304..28106)
                     /locus_tag="IW833_000343"
     CDS             complement(26304..28106)
                     /locus_tag="IW833_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1306488.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(28120..28872)
                     /locus_tag="IW833_000344"
     CDS             complement(28120..28872)
                     /locus_tag="IW833_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1306489.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(28881..29243)
                     /locus_tag="IW833_000345"
     CDS             complement(28881..29243)
                     /locus_tag="IW833_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1306490.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(29419..30291)
                     /locus_tag="IW833_000346"
     CDS             complement(29419..30291)
                     /locus_tag="IW833_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ1306491.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(30342..30830)
                     /locus_tag="IW833_000347"
     CDS             complement(30342..30830)
                     /locus_tag="IW833_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ1306492.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(30968..31759)
                     /locus_tag="IW833_000348"
     CDS             complement(30968..31759)
                     /locus_tag="IW833_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ1306493.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GKSYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(31814..32422)
                     /locus_tag="IW833_000349"
     CDS             complement(31814..32422)
                     /locus_tag="IW833_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ1306494.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(32415..32732)
                     /locus_tag="IW833_000350"
     CDS             complement(32415..32732)
                     /locus_tag="IW833_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1306495.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(32888..33616)
                     /locus_tag="IW833_000351"
     CDS             complement(32888..33616)
                     /locus_tag="IW833_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ1306496.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            33775..34656
                     /locus_tag="IW833_000352"
     CDS             33775..34656
                     /locus_tag="IW833_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1306497.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERANRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(34893..35936)
                     /locus_tag="IW833_000353"
     CDS             complement(34893..35936)
                     /locus_tag="IW833_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1306498.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(35939..36607)
                     /locus_tag="IW833_000354"
     CDS             complement(35939..36607)
                     /locus_tag="IW833_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1306499.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            36749..39085
                     /locus_tag="IW833_000355"
     CDS             36749..39085
                     /locus_tag="IW833_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1306500.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(39200..39511)
                     /locus_tag="IW833_000356"
     CDS             complement(39200..39511)
                     /locus_tag="IW833_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306501.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            39692..41167
                     /locus_tag="IW833_000357"
     CDS             39692..41167
                     /locus_tag="IW833_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ1306502.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            41214..41798
                     /locus_tag="IW833_000358"
     CDS             41214..41798
                     /locus_tag="IW833_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ1306503.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            41800..42219
                     /locus_tag="IW833_000359"
     CDS             41800..42219
                     /locus_tag="IW833_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ1306504.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(42500..42784)
                     /locus_tag="IW833_000360"
     CDS             complement(42500..42784)
                     /locus_tag="IW833_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ1306505.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(43016..43726)
                     /locus_tag="IW833_000361"
     CDS             complement(43016..43726)
                     /locus_tag="IW833_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ1306506.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(43723..45195)
                     /locus_tag="IW833_000362"
     CDS             complement(43723..45195)
                     /locus_tag="IW833_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1306507.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            45333..45806
                     /locus_tag="IW833_000363"
     CDS             45333..45806
                     /locus_tag="IW833_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1306508.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(45959..46510)
                     /locus_tag="IW833_000364"
     CDS             complement(45959..46510)
                     /locus_tag="IW833_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1306509.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            46615..47145
                     /locus_tag="IW833_000365"
     CDS             46615..47145
                     /locus_tag="IW833_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ1306510.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(47628..48071)
                     /locus_tag="IW833_000366"
     CDS             complement(47628..48071)
                     /locus_tag="IW833_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1306511.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(48087..49247)
                     /locus_tag="IW833_000367"
     CDS             complement(48087..49247)
                     /locus_tag="IW833_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1306512.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            49754..51454
                     /locus_tag="IW833_000368"
     CDS             49754..51454
                     /locus_tag="IW833_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306513.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(51556..52389)
                     /locus_tag="IW833_000369"
     CDS             complement(51556..52389)
                     /locus_tag="IW833_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ1306514.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(52693..54858)
                     /locus_tag="IW833_000370"
     CDS             complement(52693..54858)
                     /locus_tag="IW833_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1306515.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            55118..57412
                     /locus_tag="IW833_000371"
     CDS             55118..57412
                     /locus_tag="IW833_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1306516.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            57405..58070
                     /gene="pgmB"
                     /locus_tag="IW833_000372"
     CDS             57405..58070
                     /gene="pgmB"
                     /locus_tag="IW833_000372"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1306517.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            58063..59070
                     /locus_tag="IW833_000373"
     CDS             58063..59070
                     /locus_tag="IW833_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1306518.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            59092..60111
                     /locus_tag="IW833_000374"
     CDS             59092..60111
                     /locus_tag="IW833_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1306519.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(60392..61759)
                     /gene="nhaC"
                     /locus_tag="IW833_000375"
     CDS             complement(60392..61759)
                     /gene="nhaC"
                     /locus_tag="IW833_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1306520.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(61868..63289)
                     /locus_tag="IW833_000376"
     CDS             complement(61868..63289)
                     /locus_tag="IW833_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1306521.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(63532..65409)
                     /gene="tdc"
                     /locus_tag="IW833_000377"
     CDS             complement(63532..65409)
                     /gene="tdc"
                     /locus_tag="IW833_000377"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1306522.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(65700..66956)
                     /locus_tag="IW833_000378"
     CDS             complement(65700..66956)
                     /locus_tag="IW833_000378"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ1306523.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            67536..68837
                     /locus_tag="IW833_000379"
     CDS             67536..68837
                     /locus_tag="IW833_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1306524.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(68930..70318)
                     /gene="ltrA"
                     /locus_tag="IW833_000380"
     CDS             complement(68930..70318)
                     /gene="ltrA"
                     /locus_tag="IW833_000380"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1306525.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(70805..71404)
                     /locus_tag="IW833_000381"
     CDS             complement(70805..71404)
                     /locus_tag="IW833_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1306526.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        21334 a        14529 c        12690 g        22986 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgctcttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acgtttgaat
     1501 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1561 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1621 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1681 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1741 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1801 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1861 tagtcttcgt tcttcttcta tcgtcgttac tggttctgct tttatttcat taaagtcgat
     1921 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1981 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     2041 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     2101 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     2161 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     2221 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     2281 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggatat
     2341 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2401 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2461 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2521 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2581 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2641 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2701 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2761 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2821 ataattagtt aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2881 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2941 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     3001 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     3061 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     3121 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     3181 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     3241 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3301 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3361 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3421 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3481 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3541 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3601 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3661 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3721 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3781 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3841 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3901 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3961 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     4021 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     4081 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     4141 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     4201 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     4261 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4321 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4381 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4441 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4501 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4561 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4621 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4681 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4741 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4801 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4861 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttagttg taaagattcc
     4921 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4981 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     5041 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     5101 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     5161 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     5221 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     5281 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5341 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5401 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5461 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5521 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5581 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5641 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5701 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5761 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5821 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5881 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5941 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     6001 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     6061 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     6121 aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt aattttttaa
     6181 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaaaactgt
     6241 cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca tgacggtttt
     6301 gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta gcataaaatt
     6361 tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt agaagcctta
     6421 ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt tgtcttagaa
     6481 gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct ggtgacaggc
     6541 tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag atatgcttat
     6601 ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta tttatggggc
     6661 gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat ggtctttttg
     6721 ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc aatcaatccc
     6781 attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt tctaattttt
     6841 tctctcatta cgttattttt ctcagcttct tacttttcta cctatttgct acttgggatt
     6901 gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt tggaggaatc
     6961 tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt ttgtatctgg
     7021 ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa aataatacgc
     7081 caattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt ttatggtcca
     7141 tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat cacatccgat
     7201 acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga tgggtctatt
     7261 cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt acttttttct
     7321 tcttttcgac ttcgcgtcaa caattggttt acgcgctttc tttttgggtt attgacgtcg
     7381 cttttaggag atcacttaac aagcatcggc gatttttttg ttttgaaatc ttcggttatt
     7441 gaaccaggag attcattgca gtattttcac attgtcttat ccgctttttt atctgcttta
     7501 ttagcttttt gtttaaaaaa atcgttagag acttggtttc acactgtgga ccgccgtatg
     7561 atcggtacga tcgggacatt gatattattt acatactata tcattatttt ttatacccgt
     7621 ttttctggtg aaacacctga tgtcttagct ttgaatacga gttttttcat tctatatctg
     7681 tgtgttggcc tgatcatctt ggtttattat tggaaaatct ctaataagaa attagctgat
     7741 cagttattag agcaacaagt caggatgcaa caggattata tcaaagatct agaaaaaaac
     7801 tatcaagact tgagagaatt caaacatgat taccaaaatc tgcttttttc tcttcacagc
     7861 tatatttcag aaggcgactt agaaggatta cggtcttatt atgatcaaac gatattacct
     7921 acacgccaga tgatgaacct ttttccggcc aatctaagta tgttggataa tatgaaagtt
     7981 cctgagatcc gtagtttact gtcattaaaa ctcatgatgg cacaggaaaa agggttgatc
     8041 gtccagcttc attttcctga gccaatcgaa ctggatacga aacatacggt aaaccttgta
     8101 cgtatgcttg gtattatctt ggacaatgcg attgaaggag ctagtgctgc aaagaagaaa
     8161 aaaatcgaat tgaccatact caagaagaat cattccgtga tgattcgtgt gatcaataca
     8221 acaacaaata cattaccgtt aaaccagttg aaacaaaaag ggttctcaac aaaaacaaat
     8281 ccaaaaaacg aaggccttgg cttgtttatc ttggatgaat tgacaaagac caatccttat
     8341 cttttccttg aaacatcaat tgaaaaccag cgtttttctc aaacgattta tatacaaata
     8401 aaataaagga gtaatgatat gattcccatt tatatttgtg aagacgatcc aaaacaatta
     8461 gaagttttat caacggtgat caaaaataga atcatgattg aaaacttaga cagttatgtc
     8521 catatttcta cctctgatcc ggaagaactt ctcaatgcag ccaagatcag aacttcttct
     8581 tttggtattt actttttaga tatcgaacta gaagacagtg aaatggatgg tgtagcgatc
     8641 ggtaaattga tccgtgaatt agatccttta ggaaaaataa tctatgtgac ttcccatacg
     8701 gaaatgtcga tgaaaatctt aaaaagcaac atcgaaccga ctgattatat cgtcaaagaa
     8761 gatctttatg atttaaaaga aaatgtcgag catatcctga gcaaaatttt cgaagatttt
     8821 caaatgtccc aaatccaaaa agatatcttc aaaatagagt ttaatgatga gatcaaattt
     8881 ttaccgatca aagacatcca atatttttct acttcgctag gagcgccaca taaactggaa
     8941 gtccatttga ctcatgccca attacaattc tatgagaaga tcaaagaaat cgagaaaatg
     9001 catcggaatt ttatccgttg tcataaatcc tatgttatca atacacaaaa tgtgcggtcg
     9061 attaataaaa aaaccagaga agtcacgttg gcaaacggcg agaccatccc agcctctatc
     9121 cgtggattaa agaaattgat ttcataaaaa gaaccgaata gtttggaccg actaaatgac
     9181 cagccgatcc aaacccttcg gttctttttt gcctatcagt gcaaaagcat ttcctgtgca
     9241 atttcacgtc catccattgt atctggataa aatgttggcc aattctccat ttctttcagc
     9301 aatacttctt gactttcatt tcctgcatat aaatgaaaat gttcggagtc agccggtgca
     9361 atcgtgtgat cactgaattg aatatatcta aaagcgccac tttccttatc ttctgcttcg
     9421 aatagatacc ttacgccacg gtttcctgcc tcgtaggtca agatttcttt ccctacataa
     9481 cgataatttg aacgtttcgt tgttccattc ttaaacgtaa atgccatctc tttctcactg
     9541 atcttgattg aagcaatatc tgtttgatag cccttcgtat aatattcttt atattcttgt
     9601 gctgtcttat cctgattcaa tttttcttta tattcaaaaa caggatcaaa agtcccatct
     9661 tctacatacg tatctactgt ttgccaagtt cctgcccaat ctgaaagaga gcgatcttgt
     9721 acatcctcgt cttcaaaata accgttgtag acgctctttt cttttttggg ggtcaaagta
     9781 tcttctagcg ggttttctgt ctctgtcgtt tttttcaacg cttcgaggtt tgcttccatg
     9841 atagaaagat agttttctcc attttctatt tgttcattcg tcaatccttc caaaggattc
     9901 aatacttcta aagatacacc tgcttcgttt gctaacgttc tggcaatact atctttggcg
     9961 ttttcttcaa aataaatata gttgattcca tgttctttaa caaagtgttt cagttcagca
    10021 attctcgccg cagacggttc ttcactagaa gaaagaccag caatcgatac ttgattcaat
    10081 ccatattcta acgctagata gttgaatgca gcatgttgcg tgacaaaaga tctttgtttt
    10141 gcttgcttaa aggtttcttg ataaagctga tggagtgctt gaagtttctc ggtataggct
    10201 gctgcatttt ttgtccatac ttcctgtttt tctgggtaag cttcaatcag ctgatcacgt
    10261 atgtttgcta cctgcttgat tgctaaactt ggagctagcc atacatgtgg gtctagctca
    10321 tgtgtatggc cttcttcccc gtgttcatgg tcatgatcat gtccttcttc acttcccgga
    10381 agtaaaagca taccttctgt cgcttttatc gtatggacat tgccttcttg taatgttttt
    10441 tggattgcgg ggacccatgt ttccatgttt tcatcattat aaacaaagat atctgtatct
    10501 tggatctttg ccatgtcttt agcagaaggt tcataatcgt gggaatcagt ccctgctgga
    10561 atcaataact ctacttctcc ttcatccccg acgattgctt ttgtaaaatc atacatggga
    10621 taaaaggtcg tcatcacgct caatttttct ttactgtcct cggctttccc acttgccccg
    10681 caagcagtta acatacctgc taaaaccagc atggccaata tacctatcaa tcgtttcatt
    10741 tatgacactc ctcttctcat cttctttcat ttgaatgttc cgattcgctt ttcgcaaacc
    10801 gtaatagtta cgttttagca acaggattta atttatcata tggttaaaag aactgtcaag
    10861 cgttttctta ttttaattga tgagaaagtt tatatgaatg attgacgata tttttgcaat
    10921 caacctctat cattttcctt ttatacccgc ttttgtctat aatgaagtta tagataaagg
    10981 aggaaaaaga gtgaaaaaag aaacagatac aacgaatcgt ttgatccgct ttttaggagg
    11041 aaaaacctct tactatgttt taggaatagt tatcttactt gcgctgatca tttatttttt
    11101 ccatcagatc tcgtttatat tcaagcctgt cggaatcatt atcgctacta tcttgccccc
    11161 attattgttt gcactgatct tgtattatct tttggaacca gtcatcaaac gtttgagcaa
    11221 gcacctttcg aggaacttgt cagtcattat cgtttatgtg attattttat ttttattggg
    11281 gcttggaagc atatggctta ttccttttct tgaagaacag gcaaagacat tgatcagtag
    11341 tttgccagat ttgtttcaag attttcaaga gagtctccga caatttttgg agaaaacacc
    11401 ttttgccgat tcatttaatc aatttttcgc ttctattgac gacgtgacgt ctgatatagc
    11461 tggatttatt ggaaattatt gggaaagtgg cgcacaacgg atcagcaaca ttttttcaac
    11521 tgttacagct atttttatta ctttatttac tggtccaatt gtcgcttttt tccttttgag
    11581 agatcctcaa aaattttatc agtcagtttt agcgattgtc ccaccagcat ttcgtacaga
    11641 ttttaaaaat ttgacaaaga ttgctaatca acaattgggt tcatttctaa aggggcaaat
    11701 cattgcttca tttatccttg gagcagtcta ctgggtttgt ttcttactga tcggattaga
    11761 atttgctagt gttttagcta tttctgctgg gttgttatgt atcattccat atattgggcc
    11821 gtttatcgcc ttctttccag gtttgatcat tgcattccaa gactctactt ttatggctgt
    11881 caaatttgtc attgtctggt ttgccgtaca acttctccac ggtgacttgg tgattcctcg
    11941 agtaatgggt gatcgtttac agatccatcc gattacgatc ttaatcgttt tacttgtcat
    12001 gggtgatcta atgggaattg tgggcgttat ttttggtatt cctatctata cgttagtcaa
    12061 attactcgtt acttttattt tccgtaaatt caaacaacgc tacaataaat tttatggcga
    12121 taaaggagag tatgaaaact cttcattttc tgaagaagat tattttaaat aaaacacaaa
    12181 acgtcgatct tacgagaata gctcgtagga acgacgtttt gtgtttttgt tttaattttt
    12241 cagcaaagca attgcctggt atggttttag ctccagactt tgatgaagaa cagtatcgga
    12301 ataattatcg ataagaatat tggcttctgt gaatccttct gggatggcga tccttgtatc
    12361 tttatcatag aaatttgtta atacaagcag ctgctgtcct tcaaattggc gaagaaagcc
    12421 ataaacttcc ggatgatttg cttcataagc atgataatct ccttgtgcaa cgattggata
    12481 tgtttttctc agccgaatca atttcttgta ataggacaaa atagaatctt cagaagatag
    12541 ttctttttct acattaatct cttctagctt gcctccaact tttaaccaag gtttccctgt
    12601 agtaaatcct gcttgttctg tgtctgacca ttgcatgggt gttcgagagt tatcgcgaga
    12661 ttttgctttg atccgacgaa acgcttcttt ttctgtgaat ccttgttcaa ggagtaactt
    12721 ataagcattc aaactttcta cgtcaatata gtcggaaatc gatgaatagt ctgggtcgat
    12781 catgccgatt tcttctccca tataaatata gggcgtccca cggttcaaat ggatcatggt
    12841 tgctagcatc tctgctcctt gttttcgata ctgatcgata gctacaaatc gattgagcgc
    12901 acgtggctga tcatgattat taaaaaatag agcattccag ccgttaccct cactcatctt
    12961 ttctccccat gtatgaaata gtcgctttaa ttctgcaaaa tcaaatggag gagtagacca
    13021 tttattccca ttttcataat cgactttcaa atgatggaaa ttaaagacca ttgataattc
    13081 ttctctacta ggttctgtat acaagataca attttcgatc gtcgtagagc tcatttcacc
    13141 gactgtgatg atagtggaat ccgtcccaaa cgttgcttgg ttcaattctt ttagatacgt
    13201 atgggtgatt ggtttgtccg tatattcagg tttgccatca ttttctttat tatttttcaa
    13261 ttgttcgtct ttaccgataa cattgataac atcgaaacgg aatcctttta cccctttttc
    13321 catccaaaaa cgaacaactt tgaatagttc ctctcttact gcttggttcc gccagttcaa
    13381 gtcagcttgt gtccgatcat ataagtgcaa ataatatttc cccgtatctc caaatggcga
    13441 ccaagcactt ccaccaaatt ttgataccca atccgttggc tcttctctta aaatgaagaa
    13501 gtcctgataa tgctggtccc ctgctagtgc ttttttgaac cattcatgtt ctgtcgaaac
    13561 gtgattcagc accatatcga gcatgatttc tatccggtat ttctttgctt cggctattag
    13621 ctgttcgaat tcctccatcg taccaaagat aggatcgatg gctgtgtaat cagagatatc
    13681 ataaccgttg tctttttgag ggcttggata aatagggttt agccaaatca tgtcgatgcc
    13741 tagatcattc agatacggga gtttttcttt gattcctttt aaatctccaa ttccatcacc
    13801 attactgtct ttgtaagatt tgggatagat ttggtaaatg actttgtctt taaatgtcat
    13861 ggatatttac ctccattcaa ctgatttatt acctaccaca acttatatat acaagttatc
    13921 atttattatt ataactgaac cgaaagcgtt tgtaaaattt atctttatac attctaagag
    13981 gtctgttgcc caataaaaaa caagagatca gacatgtatt tgtctatcct cttgtttaaa
    14041 atagcaaaat attcttgttt cttctataaa tggtgttcaa aagcttttat ctacggcaat
    14101 cagtcttctc aaatgtaaaa catgaggagc taattttatt tgagtattct ttttgctcga
    14161 tgcaggacgg cttttttaca acctctgatt catggtgttc aatcagctgt ccttcggtat
    14221 tagctcaaaa aacggcaaaa tatgaaaaac tattttgccg tttgtttctc taatactcag
    14281 gtcaaaccgc tgatttcaca acctctgttt ttattcattc gatttcaacg catcaatcat
    14341 atcgatatgc tttaatttga aatgggtaac aatcatcaca ataaccgtaa atacgatagt
    14401 taatcctgct gataagacat aagcccacca tgtgatcact aatggaaaga tgacattctc
    14461 catagacgct tgttgcaaga tgaagtcagt caatatatat cctacaccaa atcctccgat
    14521 gattcctaaa agagtaaaga tgatattttc tcgaacaatg tacatcgtga cttctttatc
    14581 aaaaaatcca aggactttta ttgtagataa ttcgcgtaca cgctcggaaa tattgatatt
    14641 agttagatta tacaagacga taaaagccag cagtcctgat aatacgacaa agattaagac
    14701 aaccgagtct aagttcgcca ttgattcttc ttgcgtttcg atttgtttgg acatgaaaga
    14761 cgtatttgtc acagcgtctg tggcaagcag tttctcggaa agcatttctt cttcttttgt
    14821 tgtcatctta tctgtacgca gcaaaaatgc attatccatc atcgattgat cagtaactga
    14881 agcataatat tcaggggaca tataaataaa gctacctaga tagtttttca cgatttcttt
    14941 tacttcgact ttccattctt tttgatcttc atcataaagc gaaatatgat ctcctgtaga
    15001 taagtcaaat agttctgcta ctttttgcgt aatagcgatt cctgttttag gcaaggagcg
    15061 tgggttctct tcaagatcag ttaaatggat aaaacgatcc aaatcagctg ttttacttgg
    15121 tacgatcact gtcagacttt gcgaagcatg tccttttttt ctcaattcta cggtttgagt
    15181 atgaatagct aggatctcat ttactttttc ttgcttctgc agcatatccg ctgctttttg
    15241 acgactatct tccgtggatg aatccaatgt aacgattgct tgatagtcaa tgatcgttcc
    15301 aaattgtttg gctgaaacag aacttagaga atctttcaat cccacgcctg caaccatcag
    15361 cccggcacat ccagcaattc cgataatcgc cataagcatt cttgacttat atcggaagag
    15421 atttcggtaa ctgatttttt gattaaagct aaggcgtgac cacagcggag tgatgtattc
    15481 aagaaaaatg cgtttccctg gcttcggtgc tttcggttga agcaatgcag caggccgttc
    15541 tcttaaatct ttgaataaaa cgatcattgc agcgccaaat gcggcaatga agaatgcggc
    15601 agctgcttgt aaaatcggcg gccagtcata aaaaataagc gcactcccaa tatcataccg
    15661 ctcgttagac agctcaaaaa tgatccttgg caaaagattc gttcctaata tcgttcctaa
    15721 aacaatacct atccctgagg ctagacttgc atagattgca tatttttttg cgatttcaac
    15781 tttcgtataa cctaacgcct ttaatgtccc gatttccctg cggttttctt ctaccatacg
    15841 tgtcattgtc gtaaaggtga tcaatgcggc aataaagaaa aagaaaacag gaaaaacatt
    15901 ggcaatcgca gcgatacgat cagacagatc accaaattct tgaaaaccga cattttctga
    15961 acgttcattg tataggtatg atggtttagt taaattttca attttttctg catttcgatt
    16021 caattcttcc tctgcagagg ccagttcacg ttctttctca actaattgcc tttcttgttc
    16081 agcaagttga gcttcttgtt gagataatgt ttgaacagcg attgttcgtt gcgcttcagg
    16141 aagctgctgg atagttgctg actgttgcgc taactgtttt ttagcatctt ctaaccgtgt
    16201 tcttgcttgc gttgtttgtg ctttgccatc cgctattttt tgctgtgccg gttctaattc
    16261 ttcgttggcc gattttttca gctcttctaa gcgttcttcg ggtcgatctt gtaaatactt
    16321 ttctgccttt tcttgatttt gctccatttt atctttgtaa gctttgctgt acgtattcaa
    16381 attctttacg tttgtaaaat caagatataa gacagaataa atatctgttt taaaaacgga
    16441 ttcaggtaaa tagacaaaat aatcgacgct accatttccg acgttcgcgt atcctctctc
    16501 caaattgttg atgaacaatg gagaacggac aaagcccgtg atggtatact ctttttcttt
    16561 caattcatct gaatcaatct gataaatatc accgatttta tacccttcat cttccgcttt
    16621 ttcatccaat acaagctggt ttggtttttc cgggagtttt ccatcagtta attgtagctt
    16681 gttctgcttg ttatttttgt tataagaaaa tatttgaatc acttcgtttt tgccagtatt
    16741 ggaataatag atctgttttc ctgtttctac ttttgcatct gggatctttt ctgcttgagc
    16801 aatatctttt tgtgtcaagc caccagtaga aacgatttgc aaatcagaaa gtcctgctgt
    16861 accaacatag tgatccatcg ttttttgcat gacaggtcca gcagttttga cacctacaaa
    16921 taaaagtgtt cctagcatga caataaggat gattgcaatg aatcgtcctt tagattgccg
    16981 aatttcgcga aaacctgctt taagatatgt tcttgttttc ataaaatccc cctaccatac
    17041 aatctcatca atggatgaag gatgatcatt taattcaaca gaccttactt tagcatcatt
    17101 aatgtgaatg actctatcag cgatgggcgc taaagcagaa ttatgtgtaa tgatcaaaac
    17161 ggtattgccg tgttgtctgc tagcgttttg caataatttc aatacttgct tgcccgtttc
    17221 aaaatccagc gcgcctgtcg gttcatcaca taaaagaagt ttgggatttt tagctagtgc
    17281 acgagcaatt gaaaccctct gctgttcgcc tcctgataat tgggacggaa agttattcaa
    17341 tcgatgagct aggcccactt gcctcaatac ctctacagga tcaagcgcat caggagaaac
    17401 ttccgttgcc aattccacat tttcttttgc tgttagatta ggaacgagat tgtagaattg
    17461 gaaaacgaac cctacatctg ttcgtcggta agcagtaagc tgtttatctg aaaattgcgc
    17521 aatatctgtg tcatcaataa tgatctgacc ttcatcaggt gtatccatcc cacctaaaat
    17581 attaagaatc gttgactttc ctgctccact tggaccaagt ataatcacta attcgccttt
    17641 ttcaattgaa aaagaaatat cgtcatttgc tgtaatggtc gtttctccca ttttgtatcg
    17701 tttgtattca ttttttactt cgacatactt cactctgatt cctccatttt cacgatattt
    17761 ctcgctatat ctatgcatcg aatgcgtttt ctcacactta tttattttac cactgaattt
    17821 ctctagacta aagaaaggaa tcctttaatt tagttgacaa ataaacgatt tatgatttac
    17881 ttacataaaa aacattgtta tttgaaagaa ggaacatcat gaatgattta aaggaaaata
    17941 tgatttttga tttatcaaaa actgcttttg tcgcgatcga catgcaaaaa gagatcgtaa
    18001 ataacggtac actctctcca cacactgcag catctattct gacaaacaat gatttgttag
    18061 taagaacatt gaaacgtacc gctgccttga aagttttagt gaacgtagac atctccactt
    18121 tcccttattt gagtcaacaa actgatatgg gtggaatgac tgcttctgta cctccagaat
    18181 tcactgattt acttttgaag gattctttga aagatacaga taatatgttg actatcacaa
    18241 aatacaatcc aagtgctttt tttggtactt ctcttgatct tcaacttcgc cggcgtggaa
    18301 tcgaaactat cattttaagc ggtgtagcga caacaaacgg tgtatatgct actgcattag
    18361 atgcctttca acacgggtac catatcgtgc ttgctgaaga tgcttgcagt gatcgtgaca
    18421 aagaatccca tcaattgttc ataaaaaaga tatttccgaa aactgcacga gtacgctcaa
    18481 cgaaacaaat aatcgaagct atccaacaaa gcaaataagt actggcacat gtgattttca
    18541 tttacttctt tttgatttca tgggaaaata accacaggat ttagatagga gtatgaacat
    18601 ggtgaaaaaa aagatcacaa tcaaagaagt cgctaaactt tccggcgttt caatcacaac
    18661 tgtctccttg atattaaatg gaaatacgga gaaattcagt ccgaaaactg ttcagaaagt
    18721 tttagctgcc aaagaagaat taggctatca gcctgactat tttgctagac aaatgattac
    18781 caaggaaacg aaaacaattg gtgtactcgt tcctgatatc acgaatccat tcttcagtac
    18841 actgatgcga ggaattgagg atatcttgta taagcagaat ttcgtcacga tattatgtaa
    18901 tgctgattct gatcatcaaa aagaaatcga atatctagcc gagctcacac gtcgaggtgt
    18961 ggatggattc attatcgcga cttctgctgt ctctacagat gcaatcaacg aaaatttgaa
    19021 aaaacaaggc cggcccttca ttgttttgga tcagaaaaaa tctgaaggat ttagtgatgc
    19081 cgtacgaaca gacgattttc gcggtggtta cctagcgggc atgcacttgc ttagtctagg
    19141 acatcaaacg atcgcccttg tctatcctga aaatccaccg gaaaatgtcc acgctcggat
    19201 tgaggggttt aaatctgctc ttgatgttta ccaaatccct cacgaccaac tgatcctctt
    19261 acctacacag ttctcaaaac aaggtggcta ccagatcaca gcagaacttc ttgattctgc
    19321 tgcaactggt gtgtttgctt taaatgatga attagctttt ggattatatc gcggattaga
    19381 ggaagctgga aaaagcatcc ctgaagacta cagcattatt ggctatgata atatagatat
    19441 gtgcgagtat atcaagccga aactgacaac gatcgcacaa ccaatcttcg aattgggaca
    19501 aacatctgcc aagttattgt tagatcggat ccaattccca gaaaaagaat gggaagaaaa
    19561 aagattacct gtacgattag aaaaaagatt ttcaactgca cccttgaaat agctttcaag
    19621 gtgtgctata tttttatcga actattaaaa cgttttacta aaacgtttta aaaaataatt
    19681 ttcccatcaa aataaatgag atagttggag gtttgtaaaa tgaacacggt tacagtaatt
    19741 ggaagtatta atatggacac tacccttcgt ttaaccaaca tgcccaagcc aggcgaaacg
    19801 atgcatgcgc atgagatttt tcacgctggc ggaggtaaag gtgccaacca agcagtggca
    19861 gccaaacgtt caggcgcacg gacatctttt attggaggcg ttggggctga cagcgaagga
    19921 caacagttat tagatctact gacaaaagaa aacatcgata catctgggat agctgaaatc
    19981 caaggcgcca caaccggaca agcaatgatt atggtagacg cagcaggaga aaatagtatc
    20041 ttgatccatg caggggcaaa taatgctttc catgaacaag aagtcctaaa aaacaagcaa
    20101 ctgattacaa acagtgactt tattattgct cagtttgaaa gtagcttaga tgcgacgatc
    20161 ctagcttttt ctattgcaaa agacgcagga aaaacaacga tcctgaatcc tgcaccagct
    20221 cgtgaaacga ttcctactga actacttgaa aagacagata tcattatacc aaatgaaaca
    20281 gaaacagaaa tcattacagg aatcagagtg acagatcata acagtttggt cgcagcagcc
    20341 gaaaaactcc atgaattagg tattggaaca gtaatcatta cactaggcag tgccggcgct
    20401 ttctatcata cagaaaaaga acacggtatc gtccctgctt ttaaagtgga tgccgttgac
    20461 acaacagccg caggtgatac atttattggc gcattaagca gcacacttca gccagacctt
    20521 agtaacttga aagaagcgat cctttatgga aatctagcct cttctgttgc tgtgcagtct
    20581 tatggtgcgc aaccttctat cccttaccgc gaagctttag aacaatcagc agtctaaaat
    20641 taacaatgag aggaagaaac aaacatgaaa aaaggaaaag taatcaattc agatatttca
    20701 cgagtaatcg cacaaatggg gcactttgat aaattaagta tcggggatgc aggcatgcct
    20761 gtcccctttg gtactgaaaa aatagatttg gctgtctcaa acggtcttcc aagtttttta
    20821 tctgttttag aaaacgtttt agaagaactt gaggtacaat cgattgaatt ggcggaagaa
    20881 ataaagacaa tgaacccgga aatattggaa aaaatcaaat tattgttacc agatacaccg
    20941 attagcttta tcccacatga agaaatgaaa aaagagttga ataactgtca tgcatttatt
    21001 cgtacaggcg aaatgacacc ttacgccaat attatattag tgagtggagt ggtcttctaa
    21061 tggaaacgac aatgaatcct gaacagatgg tactgtccga gcagattcat atgctgattg
    21121 aacagttggg aggaaaagac aatattgatt ctgtccttcc atgtgcgact cggcttcgta
    21181 tcaaattgaa aaaacagcca gtagccaatg cagaaaaatt aaaacagcaa atcccttcca
    21241 ttaagtattt cattttactc gatagatatg cagaaataat catggatcag ggattggctc
    21301 ctgcttacaa ggaattgacg cgtcaaatcg atcattcagc tccgaaagaa aagctaaatc
    21361 cgatcgaaaa actatttggt ttgatatcgg aaatctttac gccgatcatt ccgctgtttg
    21421 ccggttctgg tatattaaaa ggtctggttg ttttagcgac tacgattggc tggcttagtg
    21481 aaacaagcgg aacttatcat attttgtctg ctgcgtcgaa tgccgtcttt tattttctgc
    21541 caatctttct tgctttttct gctgccaaaa cgtttaaagt gaatggatat attgcggctg
    21601 tcattgctgc agcactgatt gaaccgaatc tgacctcttt actttcagac agtggcaaag
    21661 cagctactga ttttctgggg attcctgtag tactgatgca gtatacgtct actgtcatgc
    21721 cggcgatttt aggtattttc ttttattctt ttgttgaaaa attcctcaaa aaatatattc
    21781 gcgaaaatat gcaattagta tttgtacctt tactttcatt agttatcacg gttcctttga
    21841 cgttaatggt attcgggcct gcgggtgttt accttggcga aggtttagct gccgcaatta
    21901 cttggatgat ggatattaac ggaccgatca gtggcgcaat catcgctggt ggttggaata
    21961 ttttggtaat atttggtatc caatgggctg taaaccctgt catgatcagt aatatctctg
    22021 cttatgggtt cgaccgaatc gttccgttga caggagcagc caattttgga atggctggtg
    22081 cagctttagg tgtatttctg cgaagtaaac gttcaaaaac ccgttcgatc tccggttctg
    22141 cttttgcttc gattttactt gcgggagtga ccgaaccaac tgtttacgga attgcgatcc
    22201 cattgaaaaa accatttgtt gctgcttgca ttggtgcagc tgctggtgga gctgtaatgg
    22261 gatttgccca agtgaaagcg atcgcttttg tatttggtag tctgaccaca ttgcctgcat
    22321 tcatttccgg tacttttttc tggtacttag caggactggc tgttagtcta gtcgtagcaa
    22381 tgatcactac tctcgttagc gggtttgatg aagacctaat gtcttatgaa tagttaatga
    22441 aaaagactga agtatgagat gtttatcaca tatttcagtc tttttctttt taggatcatt
    22501 atttttataa atttttgtga tactttccaa actttggtat aatttacata tgcataacca
    22561 tttttatccc cacttgttta tccccaaaca aaggtggtct atgcaataaa atgaacgtca
    22621 aagaaaaacg aatgaatgct cgttgcttat ttacaaattg ttttatttag aatacacaga
    22681 cagatttatg aaaagaaaaa gcttttcgac acgattatct gttccaaaaa atgtctaatg
    22741 aatgctgaat aagacagaaa atcaatgaaa ggaaaaggac tagtttatta tgaaaaataa
    22801 aaaaacgata cgtttgctag ctgctacttg gctgattgct ccgtctgttt tgtcgaccaa
    22861 taccgtattt gccgtagata atcaaatagc tgatcaaaca gtcacaacag atacgactac
    22921 atccagctca gctactagtg aaacaacgga ttcttttaca gaagacccaa ctggaacaac
    22981 taactcaaca acaggatcga ccgagccaac tgaaattgat ccggctgatc catcagaaaa
    23041 tgaaaagcca gccgagacat attcccttac aattgctcct tctttgattg gtaagatcca
    23101 actgactgct tcgacagcgg attcccctga acagcaacct ttggatatta cggaaagtgg
    23161 aacggtagaa ggtttattag cggaaacgaa ggtaacttac actattacgc cagctgaagg
    23221 tgaacaaatt gcttcagtcg ctgttaatgg tgccaaagaa gtaaattatg atgcaaagac
    23281 ctttacagta ggtacgaaca atttatctat tactgcagaa tttaaagctg gaccggatac
    23341 ttcaacagag aaaccaactg atcctagtga agaagtccct agtacaccag atccggaaac
    23401 gccaaacaaa ccagatgtgg gtaataacaa caataataat ggttcaaata attcaaacaa
    23461 taataacaat aattcgaaca gcgggaattc ttcgaatcaa acaaacccat ctggatcgaa
    23521 taacaatgga aatactcaga gagaaccaaa ccatccaaac aatggttcat cgaatggcgg
    23581 aacgaacaat gcttcatctc gtccaaatac agcaaatcaa agcggtatag agaatccatc
    23641 ttctgcttct tctgattttg ttgtaaaatc accaattgat gctgtattgc ctacaaatac
    23701 aacgaatgtc caacaagcaa tcgttcgaga agcattcaag catcttggaa aaccatatgt
    23761 atggggagca aaaggtccaa atacttttga ctgttctggt ttgacttatt acgtatatat
    23821 gaaagcaacc ggtcattata tcggtggctg gacaggcgaa cagcaatatg caggtacgca
    23881 gattccagta agtcaggccc aacctggcga cctcgtcttc tggggacctt cttccggtgt
    23941 tactcaccat gtaggtatct atattgggaa cggacaattt atccatgcgc ctcaaccagg
    24001 agataaagtt cgagtaactt ctatttcaga tttcacgcct gattttgctg tccgagtaaa
    24061 cttagcaggt ttacctgctg cttcaggttc gctgacaggc ggctcatcta ttttagatgg
    24121 attagatggc tcgttccatt tcagtcaaaa ccaaacaact gatcagttct tgaaaaagat
    24181 tgcagatgat gcacaggaaa tcgggcaaaa agaaggaatc tacgcatcag tcatgatggc
    24241 tcaagctatc cttgaaagtg gatctggaaa cagcttatta tcaagcgaac caaatcataa
    24301 cctttttggt atcaaaggat catataaagg aagcagcgtg accttcaata cacttgaaca
    24361 agatagttcc ggacaaagct accaaatccg cgcacaattc cgtaaatatc cttcttacaa
    24421 ggaaagtttg gaagactatg ccgacttgat caaaaatgga ctgactggca atcctgattt
    24481 ctacaaacca acttggaagt cggaaacaaa agactacaaa gaagcaacca aatatttgga
    24541 aggacgttat gctacagatc gccaatattc acaaaaattg aacgcgatca tcgaagcgta
    24601 cgacctgaca aaatatgatg aaccgaaaaa agaagcagca acaactgaag aagcaaacgc
    24661 tgaaatcctc aatatgtcta agcgattcga tgtgccaatc aaatggacaa cagatggcgt
    24721 gccaatcaca ggattagaat tttcaacgat aaaacgcgtc aacaacgctt cttccatctt
    24781 ccgtgtcgct tcaatctggg atttgtggaa caattttacg gcaagacaga taccagaaac
    24841 aactactcgt acagttaaag ctgcaactgt tcctgtacta accttgttaa gtttagacaa
    24901 atcattctgg cttgatctgc ctttaaagaa atagaataaa ttaaaaaggg gatgtgacac
    24961 tgtcacactc cctttttaat ttattctatt tctttatatt tattttgtat tccgttacaa
    25021 actcgcgtac ttggttaggt tccagagtga catctccaaa actttcaagt cgctccgctc
    25081 ctggcagttc ttgagtctca aatgtgatac cgccatgatt tactaattta ttcccgcgca
    25141 tttccggtcc atcttctcca aaatttgctg taaaaatcac gattcctgaa gcatctgttt
    25201 ttacttggat cgatactttt tcatctgggc ttgtcaattc cgcagcattt agtaaaccag
    25261 attgtttttt caacaaaaat ggatgatcga tgccgcccac tatcattgat tggcgaaaat
    25321 cactggcaaa gacctcacct aattttttag gacgttggaa gtcaaatgct gtgccactta
    25381 ctgaaactac ttctcctgta ggcgttgtat ctttattgag cgtagcaaat aatcggctat
    25441 tcaaccaaag tatatgttcg tcgattgact gtgctggatc accggttaaa ttgaaataga
    25501 catgatttgt aggattgaat actgttttgc ggtcactgat cccttggacc gtcactcgcc
    25561 atgtatcatc gttcgtcagc gtataccgta cttctacatc taaagttcca ggaaatccgt
    25621 tttccatatc tgggctagtc tgatagaaaa tgaccgatgc ttcgttttca ccattgatga
    25681 ctgtataatg ccactgcttg gcttcaaagc taggtgcacc accatgtaaa ccaatgacca
    25741 gtgtccgggt ctgtttttac ttcgaacgtt tcatcatcta atttgaatgt gccattcgta
    25801 atccgtccag ccatacgtcc gattgaagca ccgatataag ggtctttttc tagatattct
    25861 tttgcttcat caaaacctaa gatcaactct cgtttttcat tatcgattat tgtttgaaaa
    25921 tgaacaaccc gtgccccaag atcactgaca gacaaagatg tcccattttg attcgtgatc
    25981 gtaatcaagt gatagttttc gccaaaattt tgttcattga tttccatgtt ccttgcaccc
    26041 tcttttcttt catttgcact gattccagca tacatggaaa ccgttttctt aacagcttat
    26101 tccgtgagta acgagaaaaa atttactata ttctaataaa agaccgtgaa aaaaaggttg
    26161 tgacaaaaat attgtcacaa cctctttttc cgaacaaacg gtgttcaaaa gcttgcatct
    26221 gcggtaataa gcccaaccaa acgaaaaata tgaggagcta tttttgcttg gctgttctta
    26281 ttacttgatg caggacagct ttttcacaat ctctttttca atacgattcc agtatataac
    26341 catgtcgtca gtacatagta gatgaaatac aatacgaaga atatcgtaaa cggaacccaa
    26401 atatctttgt atggattttg catgaataga ccaaacattt gtaatccgaa taagacatgg
    26461 actacaccta agattcctgg tgctaagaat aacacgccta cttctctacg gatcgattgt
    26521 ttaagcaacc cgcgtcgtgt tcctatcttt tcaagcattt cataacgagc aatgtcactc
    26581 ttgcttcctg acaagatctt gaacatcaaa cagctagcaa gcatggtcaa gaaagctaaa
    26641 cctaggaaga atcccataaa ttcaaaacca gagaatagac cattatacac ttcatacatt
    26701 tcatatttct ggttgctgta ctctactttt tgcaaagatg gattgttcat tttattttga
    26761 gcaacaagtg tttcgattgg tttcaggttt gcttggaagt cttttacctg aactagctgt
    26821 aaagtggatt gtggcaagtt cagttgatca aattctgatt gactgactac cttgatttct
    26881 ttttcttgct gttccggcaa ttcatagctt cgaagttcct cttgtacagt cagttcattc
    26941 atttttttag caggaacttt ttctttcgtg atcttcggcg gtgttccatc ttcatgtttt
    27001 atcatgatca atggttgagc cgcaaattca cttgcattat agtaaatcgt tcccgcatct
    27061 tctttttgag tataagaagc agaaagtgtt ggtgaaagtt ctttgatttt ttgctgatct
    27121 tctgcccgag gattattcat cactagatca tatccagaat tgttttctgt caagtctgca
    27181 atttcatttt taaaacctaa tcctaccgtc aaagcgccca gtgccaaggc aaacagcata
    27241 gcgaccattg agagcatttg tgtatattca cgaatacgaa agtttaactg tgacaatgtg
    27301 aaattgttca attttttcaa tgaaatctgt tctgatttct ttagtaatga taaaaagaaa
    27361 atgatcacag aatggaaaat gaaataagtt cctaatacga tcgtaactaa ggctacccct
    27421 ataccaaaga cttgataggt ccccacgtgt ttcatcatat agtaaccgca gcctagaaaa
    27481 gcaattccca gtactacttc aaggaaaaac aagaatttat gctgcttgat tcttgttggt
    27541 gtctgatccg cacgaatcaa tgctaaaatc ggttttttga caatcgaaaa agcattgaca
    27601 acagcagcta ataaaaacaa aacagcaaaa aacaaaacag tgatcaacaa cccgtgaata
    27661 ctaaaaggtg taaaatgcga aatattgaga tctaactgat ccactaataa tcggtggacg
    27721 atacttgtca atcctacacc gacagctgaa cccactaaag tagctgcgat ccctactaag
    27781 aatgtttcag aaaaaatcat ctgtgcgatt tttctgcctt ttgctcccag catcataaac
    27841 attgcatagt ctttttgccg catcgtcatc aaaaaggaat ttgcatataa aatataaacg
    27901 aatgtgatga tccctagtaa aacagaaccg aatcggaaaa tgataactgt agccgcaacg
    27961 atcgtattac ttttcaaaaa agcttcgttg cttgccattg attcaaacat gtaaaaaata
    28021 gcagatgcca taactaatcc tgagaataac accaagtagt ctcttaaacg gctcttgatc
    28081 ccagtaagtg ataatttcca aagcatagtc aatctccccc tactgttcca agtttccaag
    28141 aatagatagt atttctcgat agaaggaatc acggctttga tctccacgtt tcacttcttg
    28201 atggataccg ccatccttga tgaataaaat acgttggcaa taacttgcag aaaacggatc
    28261 atgcgtcaca agaagaatcg atacatgatc ttttgtattc aattcgtcca tcgtatctaa
    28321 aagatcccgg gcacttttgg aatccagcgc acctgtaggc tcatccccta atagaatcgt
    28381 tggttgtgtg atcaatgcac gtgcggcggc gacccgttgt ttttgtccac cggaaatctc
    28441 agaaggatag ctttccaaaa tatgagagat ggataaacgt tctgcaattt tttggacacg
    28501 ttgcttgatt tcttttggtc tgacgccttg taaagaaaga ggaacagcga tattttcttg
    28561 tgctgtcaag ctttctaata aattgaaatc ttggaaaata aagccaatct cattcgcacg
    28621 aaaatctgct agttgatttc cttttaatgt ggtcacatct ttttgattga ttcggatatg
    28681 cccatctgtt ggtttatcta atgtggataa aatattcaaa agtgtcgact taccagaacc
    28741 agaagcgccc atgatcccaa taaattcgcc tttttctaca tcgaagctga tgccgcttaa
    28801 tgcttgcgtc tttttctcat tgttttttcc gtatactttt gttacattct gtacttcgac
    28861 tattttgttc atttctttcc ctacccttta attagttttt ccagtgctgt tttcggcacg
    28921 tctttttttt cgacttcttc atacgaagtg acacctttat ttttgttcca ggtgattttt
    28981 aaatatgctc ctttacgaag cggtcgagtc aattgtccct tgaagtccag ttctttttcc
    29041 ttaccctctt tcgtatagcc aggcaggata tatctatagt tgactgtttc ttgtccagag
    29101 tctgattttg aaactgtttt tgtaccatct gtcgtaattt gtacatagta ttcttctcca
    29161 cccataacta cgtaatctgc gattttcacc cctgcaagta ccacaacgat gaaaacaagt
    29221 agaccgatca gaagtttttt cactttttcc ccttctctct tactttatat actgattata
    29281 gaaaaaagcg aacaattctg ccttcgaatc aactatcatt cttctttcaa ttttgtcatt
    29341 ttctttaaaa caactaaaaa aagagttcaa gacaataatc cgtcctaaac tccttcttcc
    29401 tatcttacaa aaagtatgtc acttacggtc tttcatgtcg cctgtattga taacaggttg
    29461 cgttcctttg tctgtaatga ttgagacaag taaattattg atcaattgga ctttccgatc
    29521 atcggtgaaa ttgatttctt gtccatcttc gatttgctct aaggccattt gagtgattgt
    29581 gacagctcct tcaacaatgg tttgtcgagc agacaaaatg gcttttgctt gttgacgttg
    29641 gagcatggca ctagcgattt ctgttgcata ggctaaatgg ttcaagcgtg tttcgattac
    29701 ttcgacacct gctactgcta aacgctcttg cagctctttt tgcaactcgt cagacactgc
    29761 cgtcgtgttt cctcgaagcg tcaaatcatt ctcattgaat gtatcatacg ggtattggct
    29821 agcgatatgc cgaatggctg tctcactttg gatttctaca aaatcttgat aatacgctac
    29881 atcgaataat gcctttgctg tatcaacgac tttaaagact actactgctg agatttcgat
    29941 ggggtttcca tccaaatcat tgacttttaa aacagaacta ttgaaattcc gaactttcaa
    30001 cgaaacagtc attttttgtg ccaaaggtat cgtaaggaaa aagccatttt ctcgaatcgt
    30061 acctaaatat tgtccaaaaa acaaaattac ttttgcttga ttaggactga ctacagttgc
    30121 cgaacttaac aacaataacg tgatcaacca aagaaaaata cttaaaaaaa gtgcccaaac
    30181 tttcgcttgc cacataccta aatagaagaa aaacaatcca actaaagcta atacgaccaa
    30241 tccgacaatc cctgcataac cattgatata aaatgctctt ttttctctca tgctacttcc
    30301 tgcgctcctt tatccttcaa taaatatcta atgttacttt tttaattttt ttttcgaata
    30361 cgcgccatta caccttctag ttctaactct ttgtcttcag tgatgtacca accgcttgcc
    30421 tgtgtccctc cggctgcctg cggtaaataa tccaattttg ttcgaaccca gtgcttttct
    30481 tctgtaaaat agtcttctac caagacctct agatccatcc ctgctgaaag aggcaaatct
    30541 tcccgaacag atttaaagat aaatcgttcc ttcttcccat catacttcaa tattcctgta
    30601 tcgataatcg gtaaagaata ataatccagc tctttcttca ccgtcaataa atgtttgaaa
    30661 acttcatggt acacttgctc taattggcga ccttctgaat tgttcgggta attaaccatc
    30721 actttttctt tgtctggatc atagaaggtt tggcttagtt gagtgatata agtatgtaat
    30781 ctttccattt ctgtttttaa cttttctctt tgaccttctt ctaccttcat atactctttc
    30841 accctgtttt ctttttagat attttctttc ttattttact atagttatgt gcgttaagcg
    30901 atgaaaaaca gcaaagattg gaaggatctt tgctgttttt tcttaaaata tccagttgag
    30961 tacaggatca atctgtttca ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt
    31021 tagcagtgat ctttagcttt ttctgtcctt gttctaacaa agtatttgtt attggctggt
    31081 agtctttttc aatataagat ttaccagacg tttggatagt atagccactg ttttccatct
    31141 catgcaaaga taaattgcca ccgtttagga tgacttttcc ttctttgctg atcaaactgt
    31201 cttctagatg gaagttgctg ttttcacctg tgacgatcaa gttatccaca ttggcctcat
    31261 ctaatcgaag tgagcctaat tcatctttcg cagttattgt tcctcctgta aattcattga
    31321 acgataccca gccatcatgt gtcttagtat tcactttttt gattgatact tcgttgatat
    31381 caattgcact attattccct tcgattgtca attcagataa ttcatgatct gcagggacaa
    31441 caatagtgat ttgtgcactt gtgtcttcta aactaatgat tgcacctgtt gattttttga
    31501 ctgcattcac agtcaacacg ccatttttct cctttatttc tggcttttct gtcttatcat
    31561 aagtcgtttt tatctcaaaa tccaatccgc gtatgaccga aactgtttga ttttctgcat
    31621 ggatgacgat tttgtttgca tccttgattt tttttgtctc agtaaccata tcgatcaacc
    31681 gaggaccatt atcccaagtc agactctttt gagcgcctaa tgtataagtt gcacctgcta
    31741 ggaggccacc agtaagcatc agaccaatac cggtaatcaa aaaatgtttc aatctcaatt
    31801 tatgtgtcct ccttcaaacg cccgctgaat gcagatctct ttttgtaata aaacgtcgtc
    31861 ccatccattt aacaaagttc atcatgacat aaaaaagccc ttttgttcct ttcagcaaca
    31921 gtggcgaaat aaatactgcg cttcctgtgg cgaacagacc gacaccgata aagaataatc
    31981 cactcagaat cgattgtcca aacacactga ttcctgagat gatcatgaat aggccaacac
    32041 ctaagatact gatacataaa acatagatac taaaaatgag cgctagtaaa gcaaccaata
    32101 gtcccccaat aaccaatata aaccccagtg ctagtggaat gaggacagga gaagcaaata
    32161 aaccgataat gattaaccaa atcaggcgaa tttgtctttt tggatttttc actaactcgt
    32221 tttctgcttg ttcttcttct gaaaagtaac tgatttttaa ttttaaggca aactgctttg
    32281 gtgtaccata ttcatcaatg atctgatcta atgtttttcc agaatctaaa aaatattcct
    32341 cataatactg gatcaaatca aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa
    32401 cttcattcaa atattcattc atttgtgctc tcctccaata accggtctaa agaatctcga
    32461 aaaacaatcc attcatcaat aatttcttct aaatgacgac ttcccattgg tgtcaacgag
    32521 taatatcgac ggtttctgcc ttcaaaaggt tcatcatagg tctcaagata tccttctttt
    32581 ttcaatcttc tcaaaaccgg atatagtgta gattcactga cagagagact ttcttgtacg
    32641 atttttgtta aggcataccc atacatatct tccttgctta atgctgctag cacagtacca
    32701 tcaagaagaa cagttggaat ctggatgtgc attacgtcac cttctattct caagtgtaat
    32761 attttatttc aatctatatt atacaacata taatatagtt tcaaaatcag tttgtcaaat
    32821 acaaaaaaac tgactaaaag atttgatcct ttttagtcag caaaaaatgg cttctctttg
    32881 atttttatta taacagtggc ataatccaat ttgtccacat ccagctcagt ggcatgataa
    32941 cgatcattgc aggtatcatg tcagctactg ggaacatttt cacgttgatc attctaaaac
    33001 cggtagcgac catcaagaat ccgccgcaag ctttaaaatc taagatcatg tctggtgtag
    33061 tcaatggata aataaatttt gccaataaga acaaaatata aaaaataatg aattggggaa
    33121 ttgctatcac agaaacgaca taccctaaat tacacgcaaa aatagctgct gtgaaaaaat
    33181 caaggacaga tttagaaatc aaaatcgtac tgtctcctgt catcccagaa tctaaacttc
    33241 catagatccc cgtaccactt gcacaaaata gaacaatgac cgtcactaat gtgctgataa
    33301 attcatcgtg ggtcatacca agcttttcac tagggaagat tttagaaata ggtacttgca
    33361 tcagcgcagc ccccttattt atcccattac ctaaatgaac aatcaaacca atcgctgttc
    33421 ctatcactaa agcaaaaata acagccggca tatttttcat cggcgcaatg gaatagattc
    33481 ccatccccat cgaacacaca ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta
    33541 atttatttcc tactaatccg ccaattagtc cgccaaacaa aacggaacct acattgatca
    33601 aaactccagt tggcatcaaa aaactcctct ctttttctac gaagcgaaaa aaatcagaaa
    33661 aatttctgta attattgctt tcgctgcaag aaagattgta acagacagag aagattacac
    33721 caattcattt tttgtgttaa agtattcttt attggaataa gtaggaggaa tatgatggat
    33781 atccataaat tcgaagtttt tcttgattta gccgaaacaa tgaatttcac caaaactgct
    33841 gatcgacaat tcaccaccca aggaaatatt tctaaacaga ttctttcgtt agaaacagaa
    33901 ttagacgtca aattattcga acgcgccaat agaaaaatcg aattgaccga agctggaagc
    33961 ttattgctcc catatgtaaa aaatgtcgta gaacaatacc atgctatgca agaagtgctt
    34021 gaaacctata cgaaagacaa caatctttca ttaaatatct taacgatacc gacaatgatc
    34081 aattataaag gtttttccaa aatcaccgag tttttgaaac gccatcctga atttacagtc
    34141 cagttgaaag aagtggaaag tgtcgaatta acttacaatg acgccaatga acatagtgac
    34201 acgatccact ttgctagaag tttccaggcc ccaccaagtt cgattgagtg gttgcctact
    34261 gaaacagacg attttgtagc agttctacct aaaaatcatc ctttagctag taaaaagaaa
    34321 ttggatctaa gcgaactgaa aaaggagaac tttttgctcc ttggccccac tacgaacttg
    34381 tatcaacctg tactagatct gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa
    34441 ggagcaagaa tcgacttgat catcaatatg attgcaaatg acatggggat tgctattgtt
    34501 atggaaaaaa cagtcaagaa tctattaaaa gaaaatactg tgattctccc tatttctcca
    34561 acaacagaga gttatcttgc atttactcgt aaagtcggtg agcattcttt ggcttcagat
    34621 acattttggg agtatttaaa tcaagaagaa aactaagccc tacattttct gtccatctat
    34681 ccacccaaaa aaagagagcg acgcatgact ttcgctctct ttcttgcatc atgtccttgt
    34741 ttcaatgaca cctctaacat ttctcgtagt tcaataagaa agcagcgatt tttatgccaa
    34801 aagaaccgta agaacatttc cctaatcaca aaaaaacgac taaaaacccc taaaaacctt
    34861 caatagaatt tttaggggtt ttattctttt tcctattgtt tacttaaccg tatcattgct
    34921 gtcgttccta ctccttcgat actgctataa tcaatacgtc catcatgcag ttctagaatt
    34981 tttttaacca gtgaaagtcc tagcccattc ccagaaattt gacgacttgt atctccttga
    35041 taaaattttt caaacattcg atctctggtc tcttcattca ttcctacacc tgaatcggtc
    35101 acttccagta caatctctgt ggctgtctca aatagtttga tatgaatctg tccattgatt
    35161 tgattgtact ttatggcgtt atccatgata ttcagccaca cttggtacaa aaactcttca
    35221 ttgcctgtgt aatttactgc agcaagttcg atatccaatt ctagctgttc tttctcccat
    35281 ttaggctgaa aaaaaacgat tacttctctg atttgttcat ctaatcgata ctctttcttt
    35341 tccagttgga tacgctggtt ttccagtttg ttcaatttta agatgttctc tgttaattgt
    35401 gaaagctgcg tgatactttc gataatccga tataaaaata tttggcgttc ttcgtcagag
    35461 agatttgggg cttgtaacaa ttgaacatat ccttggattg ttgctagtgg tgttttgaat
    35521 tcatgggata cactggacac aaagtcattc ctcagtgtct cgatactatt gagttcttgg
    35581 accataacgt tgaaatcttt atacagttgc tgtacttctg ctactttctg ctgttcatcc
    35641 attcgaatcg aaaaatctcc cgttgctacc agactcatat ttgtccctag cgcactgatt
    35701 ggatgcagga tacgccgccc aacaaaaatc gatattcctg tacccacgac catactgaac
    35761 cccgcaaaga cgaataacgg gtataacgtt ggttttccag ccgattcagc aggcgagata
    35821 gaatgtcgag acaataagaa aataaaaccg agaaaacaca gcagtgtcac taagatcgaa
    35881 atgaagacaa tactagcaaa atagatccat aattgtgaaa atagcttttt tttcatcttt
    35941 aaataaccgc cttatagcct agtccccgta ctgtgatgat ttgaaaatct tccgtctttt
    36001 caaaacgggt ccttaaacgt ttgatatgta catcgatcgt tcgttcttct gtattcgtat
    36061 ctaatcccca aatatcatcc atcaactgtt ggcgagtgaa gatcttgttg ggataagaca
    36121 gtaatttata cagaagcaga aattcttttt gaggcagaat caacgaatgc tgatcttgta
    36181 acactgtata agcatctagc tctaattttg tttgtccgat tatcaagcta ctgctgtaat
    36241 tgatctttga acgtcgaagc aatgcttcca cacgtaggat catctcattg acatcgatgg
    36301 gttttgtcat ataatcatct gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg
    36361 cctttgccgt aatcattaga acaggtgtgt caaatctggt gctacgtaat gagtctagca
    36421 agtcatagcc atccattttc ggcatcatga tatctgtaat gattaattct atacgtgtcg
    36481 cttctagttt tttcagcgca tcctctccat tcaatgcggt gatgactgaa aagcccgctc
    36541 gttccaacac agaacggtaa agcaactgta attgcggatc atcttctact actaaaatac
    36601 gattcattga tctccctctg ttttttgaaa aatttctaga ttttttcttg tagttcatct
    36661 tcagttaaca tttatttcgt atactacgat cattgttctg atatacttat tagtataccg
    36721 aatatttcaa tacgggagaa gggtgattat gcttcaatta aaagatataa aaaaatatta
    36781 taaagtcggc gaaaccacca ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa
    36841 agaattcgta gcgattttag gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat
    36901 tggtggacta gataattatg attccggtga tatggtaatc aacggaaagt ctactaaaga
    36961 ctttaaggac agtgactggg atgcctaccg aaacaactct atcggtttca tttttcaaag
    37021 ttacaacttg attggtcatc taggcattat tgaaaacgtt gaacttggga tgacattaag
    37081 tggtgtttct aaagacgaaa aacgaaaacg ggcagaagat gcattgcacc gagtcggctt
    37141 gacaaatcat atgcataaaa agccaaatca gttatctggt ggacaaatgc agcgagtggc
    37201 catcgctcga gcactagcca atgacccaga tatccttttg tgcgatgaac caaccggagc
    37261 gttagataca gaaacaagta tccagatcat ggaattgatc caagagttat cgaaagaaaa
    37321 attagtgatc atggtcacac ataatcctga actggcccat caatacgcag atcgaatcat
    37381 tgaattttct gatggtaaga tcttaacaga ttcccatcct cacatcgaac gaccaaaaga
    37441 tgatcaattt aacttacgca gaacaaagat gagtttttgg acagcgttaa agctttcctt
    37501 caacaatatc cgaacaaaaa aaggacgtac tttcttgacc tcctttgctt ccagtattgg
    37561 gatcatcgga atcgcgatcg ttctgtccct ttcttctgga ttccaaaaac aaatcgataa
    37621 cacgcaagct gaaacgatgg cgaaatttcc gattactatt tctaaggtaa cgactaacca
    37681 gactcgtgac gatgccggat tgggtgcaag caaagcggat tatccagaca gcaaaacgat
    37741 tactgcaaaa gtcagcgaag aagataaagc acagcacacg aataagattg atcaaacata
    37801 cgtcgattac gtaacagaca tcgatcctaa cttaagtaat aatatcggat ttacccgtac
    37861 tacaggaatc aatcttttac gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa
    37921 tcaaaatcct gatacggaat ctctttcttt atctagtacg atgtcagcaa tgacaggtgt
    37981 gggagtgtca agtttcccta cacagcttga cactagcaaa gaaaactttt taaaagacaa
    38041 ttattcttta cttgccggaa gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga
    38101 cggcaataat aatactaata tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga
    38161 cgaaaaactt gattttgatg atatcgttgg tacaacattc aaattagtaa acaacaatac
    38221 ttactataca aaacttccaa ctgggaattt tatccctaat acagattatg atgcgatgta
    38281 tcagaatgct tctgatgaat tgaagatctc ggggatccta cgcgtgaaat cttcttctac
    38341 aatgaatctc ttatctccag gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa
    38401 cgaaaacaaa gaatcagaaa tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac
    38461 tactgaaaaa gttgacgaaa atgccaaaca aacattgctt agttatttag gtggagacag
    38521 cttgccttca agtatcatga tttatccgaa caattttgaa gacaaagaaa agatcttgga
    38581 ttatctagat gattacaata aaggcaaatc agatgaagat aaaatcattt atactgattt
    38641 agctggaacg atgactgaac tgactggcgg attgatggat gctatcactt atgtccttat
    38701 tgcttttgca gggatctctt tagtaacaag tatgatcatg atcagtatca ttacttatac
    38761 atctgtgatt gaacggacaa aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa
    38821 agacatcact cgcgtatttg acgcagaaac gtgtatttta ggtatttctt ctgggatatt
    38881 aggtgtgttt attgcatggc tagctacgtt ccctatcaat agcatcttat acaacatgac
    38941 ggatctaaaa aatgttgctc aactaaatcc tgtccatgcg attattctag tgatcgtctc
    39001 tactatattg acgatgcttg gcggtcatct tcctgcacga atggcagcga aaaaagacgc
    39061 cgctatcgct ttgcgtgcag aataaggttg tgacaaccac atttagattt gagtcataag
    39121 agcgctgaaa tagtagaaaa cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc
    39181 tgtttttttt aattattttt tataattctg gtatactttt ctttcctttg taccacaact
    39241 cttctggcgt atacctgtct tttccattta tgaatactgt tccatctgct tcaatcgtta
    39301 cttcagtccc aatccacata ttacgagaaa gtgcgagaag attttttctt tttcctcttc
    39361 ctttttttcc aaattgcaag gcatcggtta tctcttctgt ccaatgagat ttcacgtatt
    39421 ttacttctcc gttttcaaat gtaactttca aaatattcgc tatattttcc acttttactg
    39481 ctttaggaaa tgactcctcc atttttttca ctttccttcc tccaacacta agtatgcgct
    39541 ttcttttagt gataagtata tcatagttta ttctattatc aaaaaaacgc atccattcca
    39601 ttgtagaata ctttaccgaa caagttgaat tgttttcctc tttttcacct tttactatag
    39661 ataggtaaag acaactaagg aggaatctct catgacagat caaccgtatg atttaagaaa
    39721 agtcatcgaa gaactaaaaa cacttccagg tcaatatcac gaaacagata ttgaaatcga
    39781 tcctgaagca gaattatctg gtgtttaccg ttatatcggt gctggcggta cagtaaaacg
    39841 tccgactcaa gaaggaccaa ctatgacttt caacaatatc aaaggtttcc ccggcacacg
    39901 tgtgaacatc ggaacaatgg ctagtcgtaa acgtgtaggt gaaattttgc accatgatta
    39961 tcgaacactt ggtcgtttct tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt
    40021 agacaaagca gacgctccag cacaagaagt cgttcatcta gctaccgatg cagacttcga
    40081 tattcgcaac attttgcctg cacctacaaa tactcctgaa gatgctggtc catacatcac
    40141 aatgggtgta gtcttaggat cagatccaga aaaaacaatg agcgatgtaa cgatccatcg
    40201 tatggttcta gaagataaag atacgatcgg tatgtacatc atgcctggcg gacgccacat
    40261 tggtcacttc caaaaacaat tcgaagcctt gaataaacca atgcctatca ctatcaatat
    40321 tggtttagat ccagcaattg ccattggcac gactttcgaa ccacctacca ctccacttgg
    40381 ctacaatgaa ctatggtgtg caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg
    40441 tatagcagtc gatgaagtgg gtattgctcg tgctgaattc atcattgaag cagaaatcat
    40501 gccaaatgaa acaatgcaag aagatatcaa cacgaactca ggaaaagcaa tgccagaatt
    40561 tcctggctat actggggatg ctaatccagc tgtacaagta gtgaaagtca aagcagttac
    40621 tcaccgaaaa gacaatccaa tcatgcaaac aacgattggt cccagtgaag aacacgtatc
    40681 tatggccggt attcctacag aagcaagtat tcttgccttg gtcgataaag ctattccagg
    40741 aaaagtagta aatgtctaca atcctcctgc aggtggtggt aaattgatga ccatcatgca
    40801 aatccgtaaa gaaaacgaag cggatgaagg tattcagcgt caagcagcta ttcttgcttt
    40861 atcagccttc aaagaattga agacagtcat tttagtcgat gaagatgttg atattttcga
    40921 catgaacgat gtagtttgga ctttcaacac tcgtttccaa ggagataaag atatcatcgt
    40981 gatcccagga atgaggaacc atccattgga cccatcagaa ttaccagaat ataatcctgg
    41041 ctctattcgt ttccgtggga taagtgcaaa aactatcttt gacggtacag ttcctttcga
    41101 tatgaaagaa cattttgtac gtgcaaaatt ccaagaagta gaagattggg aaaaatattt
    41161 gaaataatga atgatctgca actgttttga catcataaaa aggagtgcgg aaaatggaac
    41221 aattggcaca acggaagaaa aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt
    41281 atgccatcaa cctagtgaag aaactaaaag aatatccgat gatagaaaca cacggaatca
    41341 tcagtccatg ggcaaaacag aacttaaagc tggaatccga cttatcacta actgaattcg
    41401 aaagcctgtt tgattaccat tattcaaaca aagatttagg tgctgctgtc gccagcggat
    41461 catttttgac cgatggaatg atcatcgtac cggcaagtat gaaaacagtc gcaggtatct
    41521 ctgttggttt aggcgacaac ctgattgcac gtgctgctga tgtaacaatg aaggagcaac
    41581 gaaagttagt cattgttcct cgtgaaacgc cattaaatgc cattcacttg gaaaacttga
    41641 ctaaattagc acgactaggt gtgcaaatca taccaccaat tcctgctttt tataatcagc
    41701 ctcaaacgat ccaagatctt gttaatcacc atacaatgaa acttttggat caatttgaaa
    41761 ttgtaaatga gcattcaaaa cgttggcagg gtgattaata tggaaaaaga aacgatagtt
    41821 gaagtaacca aactaggttt cacgatccaa gctgctgtta aagtaatcgg taaagactta
    41881 ttgatcactt taacaggcgg agatacacca catatcggaa cagtcactac attttctcgt
    41941 gatacagaga tgcaaactgt acgtttccct agccacgatg gtcggttcca taaagacgat
    42001 gtcttagccg aaaaaatagc tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc
    42061 acttccggta tccatgttga ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg
    42121 tcagaagagt taggttacaa gattttagat tggttggaaa aaatagattt ccatatcaaa
    42181 gaaccagttt attataaaga tggtgaaaaa ccattatgaa agaagaggtt gtgaaatcag
    42241 cgttatgacc tgagtatgag agaaaaaaat ggaaaaatag cttctcatat tttgccgttt
    42301 ttgagctaat accgagggac agctgattga acgccgttta tcaagaggtt gtgataaagc
    42361 cgcttcgttt cgagtagtaa gaaaaatttt gtaaacatgg gtttccacat ttttgcataa
    42421 atttgactta ctaccgagaa actggctttt gaacaccgtt tatcaagagg ttgtgacacg
    42481 tcttttgtca caacctcttt tatttttgct tcactgtaac tgctacaata tcaccgattt
    42541 cttttccgat gtttttacga atatcttttc gtagtccaag gatgtagcag actgaaccat
    42601 ctggattttt cacgcccata ttcacgatac tgccttcata tggcacattg tcaaaagttg
    42661 cttggacttt aactcttcca gcaccgaatt cttttcttat atcatacgga aaaataatat
    42721 atgccccgcc cttgccgact tcggaagcat agatttttga ttcaaaagaa taaatttccc
    42781 tcattttatt tctcctattt tttacatcta gtatagcata gcaaaaaaaa gaaggaactg
    42841 tgacaaaagt cttgtcgcag ttcctttttt cgaataaacg gtgttctcct gccaactcct
    42901 cggtcacaag tcacaatagt caacaaacat gagaagctgt tttttgaaat tgctccttgt
    42961 gactcagagt taaacggcag gctcacaacc tcttccttaa acggtgttca aaatattatt
    43021 cgccttttgc ttctttcact aattttgcga atgcttgttg ttggaattca gcagctaagg
    43081 ctttcgtacg ttgttcttgc tctgctttgc tcatttttgg attttcagtc attgctttta
    43141 ccataccgtc ttgaacataa gcttttgctt cactttccat cgtactttgc tgtttcttca
    43201 ctaattcagc taacttggct gcttggtccg tacttaattc tacccatgtc tctgggattt
    43261 tgaatgtatt cactcggctg tcatattcgt gattgttatc agcaatattg aaccagaatt
    43321 tatacatgtc gtttttgtat tcccaataag tagttttcgt cactttttca gcatcgccgt
    43381 ctgtcttttc tactttattt gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt
    43441 tttcatttgt tcggtaaaga tacacttttt cttttccatt ccctaaagct ttatacaaaa
    43501 gcatatctgc tccttgggaa tcagcactgg aaacaatttt ttctgttttt gtttccgtta
    43561 ctttttccat tccaaagtga ttggacaaat tagcgacgat caaacttagt gaagcgacga
    43621 agattagtcc gaaaatcaaa gataaaaagg tttgccatgt ttttttagcg aacacgttcg
    43681 ttaaagcaaa agcaagcaag ctgacaatca aaactccaat aatcatgcat tatcccccgc
    43741 tttcattaga acaggttctt ttttcttcaa taaaaaggca ataataaagc caagaatccc
    43801 aaagatgatc gctacgataa acgcggcatg gtaacctgac agtgtggcat tgattgcctg
    43861 atctttataa gctaatggcg tattatccaa tacagatttg cttgggatat ttgcttttgt
    43921 cacattcgtc aacacactga tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa
    43981 tgtattattt accgctgtcc catgactgat caagttattt ggcaatgcat tcataccaga
    44041 agtcgttacc ggcatcatga ccatggagat cccaaacata cggatcgcat aaaggatcac
    44101 gatatataaa actggagtcg tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa
    44161 aatgaacatc cctgtgatgg ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt
    44221 cacaggcatc atagccccca tgatcaatgc acccggcaac aacatcaagc cagaatggaa
    44281 tgctgattct ccacgaatat tttgaatata caatggtacg accatttctg cccctaccat
    44341 cgccatattt gttacaccag ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac
    44401 acgaagttcc aagaatggat gttccaaatg taattgacgc catgagaaca aggcgatgaa
    44461 aatcacacca acaactaaga aaccaattac tttcaagctt ccccagccgt catctcctac
    44521 actagaaaat ccgtaaagta aactaccgaa tccaatcgtt gataaaatag cggataaaat
    44581 atcaatgcta ggatttgaca atggaataac acttttcatt aggaagaatg ctaaaaccaa
    44641 gacaagaaca acgattggca gtaccatacc aaacaagtca cgccaatggt agttatcaat
    44701 gatataacca gataatgtag ggcctaaagc aggagcaagt ccaataacga tccctgacat
    44761 ccccattgct gatcctcttt tttctggcgg gaaaatagat agcatgatat tttgcaataa
    44821 tggcatcgat actcccacac cgcaagcttg gatcaaacga ccgattagta atgtactaaa
    44881 attaggtgct gtaaagcaag taatcgttcc gatcaaaaag atcgtcatgg cagaaagata
    44941 taaatttctt gaactgaatt tattgatcag ccatgctgtt attgggatca tgatcccgtt
    45001 gactagcaag aaaccagtcg ttaaccattg cacatcagac gctgaaatat cgaaagcttt
    45061 catcaatgta ggaaaagctg tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc
    45121 aattaataag acaacaacta aaagtgtccg attatatggt tttccgtaaa tatctacggg
    45181 ttggttgtta ctcatatttc tccttctttc tgaatttttt ctgaaactac tagaatactt
    45241 tagtcctaaa ttcgctattt gtcaacctac ttgacacata agttaatacg gatataatta
    45301 agggaagcaa gaggacaaaa ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt
    45361 tggaaaatga tcaattggtt gttggcatca gtgaattaag tgatatcgta ggtgtgtctc
    45421 cacgtcaatt aaggtattgg gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga
    45481 attgtcctag aaaatatcgt ttacctacag tcataaaagt agaactgatc aaaaaatatt
    45541 tagatgacgg atttactttg tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa
    45601 tgcaccatgt ccgtaaagtt ttttcaaaag caatcaaaga tatcgaattg atcgacgaac
    45661 gctatacgat tttacaaatc ggtaacttca aagaagagaa tgctaagatg tacatcattc
    45721 atgacaacga gacagacgaa ttaacttaca agctccttcc tgtcgatgaa aaagctgact
    45781 acgaacagtt gttaaaagaa ctctaaacgc cactaatctc ctaagtacca agctttttaa
    45841 taagtagatg ctacataaat acttgaaaaa aggcctgttt gaacataaga atatccgaac
    45901 ggtgatctgc gtttctttca tatagaaagg aattctccat cactgttcgg atattttttt
    45961 atttttttcg gttactttct tctaatacat ctgttagcca atttgcatat tctgtttccc
    46021 gattgatcgc atgcgctaaa atcaggtagt gtccgtaatt ttcatgtttt tctttttcag
    46081 taggaaagat cgtttttttt cgctctaata gatgaagtcg tttttcttca tgaaggtcac
    46141 gctgctgctg aataatttct actaatgccg gatcattcac gtctttgacg aaatacaatt
    46201 tcagaacaaa ttcgtctcga ttaaccggta gttcattggt gggtgtatga atccactctg
    46261 ccaattgttc cttcccttct tctgttaatt cataaacttt cttttccaat ttttcacctg
    46321 tgatctctac ttgatgctga atgattcctt gctcttccat ttttgcaagt tcaggataaa
    46381 tctgactatg ttttgcttgc caaaattctc ctatttcatt tttgaacgat tgattcaatt
    46441 catatcctgt catcttcttc ttacttaata accctaacaa aatatatcga agtgtatttt
    46501 ttcttgccat cagttttccc tccttgacaa atctttaaat ccattctatc ataaatgagt
    46561 aatcgaaaac atgtagttta ttacatgttt taatttataa ggagatgctt aaacatgaaa
    46621 gaatttaaag aattggaaga ttttattgga actcacttta tctatacgta tgacaatggt
    46681 tgggaatatg aatggtatgc caaaaatgac catactgttg attaccgtat ccatggcgga
    46741 atggtcaaag gccgttgggt aaaagaccaa gaagctgata tcgttaaatt gacggacggc
    46801 gtattcaaaa tcacttggac agagccaaca gggacagacg tggcattgga ctttatgcca
    46861 aatgaaaaga aacttcacgg aatgatcttt ttccctaaat gggttgaaga acatccagaa
    46921 atcactgtta ctttccaaaa cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat
    46981 gaaacttatc caaaattact tgttcctgaa tttgctacga tcacttatat gggtgatgca
    47041 ggtatagaca atgaagatgt tattagcgaa gaaccatacg atggaatgcc agatgatatc
    47101 cgtagcggaa aatacttcga tgaaaattat aaacgagtaa aataaggttg tgaaagagag
    47161 gttgtgaaat cagcgttctg acctgagtat tagagaaaga aacggaaaaa tagttcctca
    47221 tgttttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcaaga
    47281 ggttgtgaaa tcagcgttat gctttgagta ataagaacgc tagttagaaa atagcttttc
    47341 atattttcta ggctagtggg cttattaccg agaagcagcc gtttgaacac cgtttatcaa
    47401 gaggttgtga aatcagcgtt ttgacctgag tattagagaa acaaatggaa aaatagttcc
    47461 tcatattttg ccgttttttg agctaatacc gagggtcagc tgattgaaca ccgtttatca
    47521 aagattgtaa caactgcata ttcacacagt taaattagtg acttcaaaat gaaataatgc
    47581 tcccagcaaa aaattaggct gggagcatta tttcgttatt ctgttcatca aaaatcagcg
    47641 ttttgattcc atttcatcac aagttcctcc tcatttactg cttcatcttt ttgctgtgtt
    47701 tgaattgtaa aaccttgtga aagatagaaa tgaatagctg attgattttt ttgatagact
    47761 gtcaaagtca gtgttttatt ggtttctttt atttttttca ttagttgcgt accgattctt
    47821 tgttgccggt attccctttt cacaaagatt cctgcgataa actgatcaat caccccagca
    47881 aaaccaataa tttcttgatc tatttctgct aagtaaatca ctgcatctgg taaagctttc
    47941 ctaacagatt ctacttgatt ctcccaataa gttgatggaa tgaattcatg agaatccaag
    48001 ttagcatgca gccaaatcga tagaacagat tccagttctt tggcagacaa ttttgttgtt
    48061 gtgcggatca ttttttctct ccttacttat tcattgattt taaaataaaa accatttttt
    48121 tgaagctctt ttacaagttt tcgcccctca aaaaagtata tcttctcttg ttttactttt
    48181 ctaataccaa aagaaacagc aactttagga tcatcagctg aaacgattcg ataagttcca
    48241 ccaggcaatt gatccagctt gactttccca tgatggtcgc tttgtactga aaacggcaca
    48301 ccatttctag taagtgtttt tttcttatgt ttcaaaatga atgaactacc atttaccggt
    48361 ttccctgacg aatcactaat aataaatgaa aaatcaagca cttttttact taatctatac
    48421 tgatagatta gtcgtccgat aaacactaac agtatgacta cagctattat cgttcctgca
    48481 agaataaacc attgccgcat ggttctttcc tgatttcctt ttccttcttc tttttttacc
    48541 gattcgttat aaggaatccg atgtcctgtc actagcaaac gatgcgtgtt gatcatatac
    48601 ggcgtacatg taagcaaggt aactaagtct tctccttctg aaatcgttag aacagacgtg
    48661 tcatctggca gtaccacttg tatattttta acttgataag ccaatttctc ccccaaaaca
    48721 gtcaaaataa acgtgtctcc gttttctaaa cgatctaatt cggtaaataa ccgtcgatta
    48781 ggtaaaccgc tgtgagcaga gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag
    48841 gttccttgca agacgcctgc tccattttca aggatcctac cattgagccg atcataaagt
    48901 gaaatattta attggatctt aggtatattg atacttccga tcagccattc ctctaattgg
    48961 ctggatgttg tactttcatt tctgttcttt tcattgaaag gatcagtacc aggattgaat
    49021 ccttggtcag ttaatcgttg gtttttctcc tccattttct ttcgctgcac ttcaatttca
    49081 gctgcggtcc gtttttgtac ttgttcgata cgtttttgat caataaaact atttaaagaa
    49141 tccacataaa atggatataa agcgatcagt aaccctacga cgatacatat taataaaaac
    49201 aaacgaaaca aaagtcgacg agactgtttt tgcttactct gcttcacatg tgtcctccct
    49261 tgcaagtttt tttgttcttt cttttattat atgggttcag gaaaaaaata gaaaatgaaa
    49321 gccgatgagg ttgtgaaaaa gctgtcctgc atcaagtaat aagtacagcc caaaaaaata
    49381 gctactcata ttttttgttg ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    49441 tttaaggaag aggttgtgaa aactgcgtta tgctttgagt aataagagcg ctagtcagaa
    49501 aatagcttct catattttct aggctagtga gcttattacc gagaagcagc tgattgaaca
    49561 ccgtttaccg agaggtcatg aaaaagctac tttgtttcga gtattaagaa aaattctgtt
    49621 tcaaaacaag gagatgcaac aaaacttatg tcgcatcctc tctccttaga actttcactt
    49681 atgtttttct tgtaaaattc ataaatattt tcatgaattt tctaatttct tgcctacctc
    49741 tcgcatagga tatatgatga atttatctaa agtttgcaaa ggagttcgac aattgaaaaa
    49801 atatggccca tttttagcaa tttttttcat tgctttggca tttctcatgc ctcatttatt
    49861 cacacataaa ctaattttgg gtgctgatgc cccattccat tacaatcggt tctatgatgc
    49921 cgcgcaacaa attaaatatg gggatttcca ttattttcct tccctttatg gattccagca
    49981 aagtggcagg atcgtgaatg ccgtttacgg cccatttttc gcttatttac aaggattgat
    50041 cgtgttgctt gcaggtagct ggtttcatta tcagattctt tcaaatttca ttctttatct
    50101 tatttcagga ttttctatgc tgattttact gagatatgtc aatgttcgaa attgggtaag
    50161 tgtaccagta gccgttttat tcatgagtac gtattctatt caatactggt ctatcaacca
    50221 aggattcact agctggggga cttgttttta tcctttatgt atgatcccta ttgctgattt
    50281 cgtccttcat aaaaagttcc ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca
    50341 gatccattta ctgtcttcga tcatgttagc tttgatgtat atcccttttt atctttatta
    50401 cttttttaac cagactgaaa aaggaaaagc actgcttcaa ctgattaagt cggtcttgct
    50461 ttattttgtt ctgacagcaa atatttgggc aggattagct attgtttaca gtgggaataa
    50521 gattcttcct ccctttgtta acagagaaat gagtcaaaag gcgatagatc tggatggcag
    50581 ttattggctc cattacccta aggcctttcc tatattgata ttatcaggtt ttttgctttt
    50641 tattttcttt caacaaaaga acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt
    50701 tttctttttt ctttctacta gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc
    50761 ttttattagt ttgatccagt ttccatttcg atttttcgtg ccatttacgc tattgcttct
    50821 actttacctt gccctttcac tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag
    50881 tatatttggc attcttattt tttcagcaca gacgatccag aacttatacc agcatctgga
    50941 aaagtgggaa aatgaaacat ttgtcagtcg acatacttat ctattcgata cgccggaaga
    51001 agcaaggaaa acattcttca gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac
    51061 cacacctgac tatctgccaa tctataagga gacaaaagac aataaatacg accggtataa
    51121 cgaagaaata ctagaaaacg aaacaacttt tataaaaaca catccaggcg gtcttttgac
    51181 aatcaagtgg aaaaacactg gggacaaaac tgttcatatc cctgttatcg tctacgatcg
    51241 gactgtgcta cagcaaaatg gaaaaacatt gactgattat gaagtaacag atatcgggac
    51301 cccaatcgtg aagcagcaaa aaggaatcaa cgagctcacg cttcattatc aaacgccaat
    51361 ctatttctat ttcattcttt ctcttacttt gattggctgg tttaccttgc tatgtttatt
    51421 tatttatcat agatacaagc ttttacgtgc ttaggcaaaa gggataaata catccgatca
    51481 aaaaactctc caaacggtat aatcgtttgg agagttttat tttaatcagt ttttttatgt
    51541 tatatgttat attttttatt cgttcacatc taattcgatt tccaccccgt agtgatcact
    51601 gatgattgga ctatcgatcc catcaaaaat ccgttgatat tttttaacct gcattccttc
    51661 agggacgaaa ataaaatcta tacgcaattt ttctgtattt tgttcccaac catctatttt
    51721 cttttccaca gtagctgctt ttcctttttc attcgcgaca acatacgaat cttgaagaag
    51781 aaggcaactt tctcttacag tataataacc ttgagagtct actggattat tgaaatcacc
    51841 taggaaaaaa agtggtacgc gagtttccaa aaagtagttt tccaagtttt tccactcaaa
    51901 ataaaagcca ctttttttat ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc
    51961 ctgatcatct atctttgttt tcccaatcaa gatcttgcgt gtctgataat cttctttctt
    52021 ttgaaattgg gagacggtaa ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt
    52081 tccttcttca tatttatcat aaccgatgtg gctcatttcc caactccaaa aatactcttt
    52141 tccgtgttcc tttaaaagct gaactaaacg gtaggcaaaa ttatctttac ggataggtac
    52201 ttgattcttt accggacaga acttcattag cttcgcaggt tctacttctt cagattccag
    52261 taattggttg atttcttgta aagcaatcac ttcataatct tctaacagga tcttatctgc
    52321 taattcttgc aattttttct ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat
    52381 gaatttcatt ggttttctcc ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt
    52441 tgtcacaacc tctccagcaa atagtgttct cctgctaact cctcagtaat aagctcaatg
    52501 gaccaggagc tattttttgt ttggctgttt ttatcacttg gtgcagggca gctttttcac
    52561 gacctctcca ctaaacagta ttcaaactgc tgcttctcgg taataagccc aaccaaacga
    52621 aaaataggag gagctatttt tgtttggctg ttcttatgac ttgatgcagg acagcttttt
    52681 cacaacctct ccttacattt taatggagcc gatcacttta ccagctgtca ctgtacctaa
    52741 ttgcttgatc actacttctt ctactttatc gtttgtaaat acgatcatga tcgttggatc
    52801 tttttcttcg ttttggatca catcaagatc catctcagct aatttcgttc ctgttcctac
    52861 ttcttggcct tctttgacag caatctcaaa agctggctga ttcatttgga ctgtatcgat
    52921 tcccatatgg atcaaaactt cgataccatc tttcgtttcc agcccaattg catgtttgct
    52981 agggaaaata cttgtcactt ttccagcgat cggcgcaaca acttctcttg atgttggaat
    53041 aacagcaaag ccgtcaccca tcattttttg tgagaatacc ggatctttta cttcagataa
    53101 aggaatcact tctccagaag ctgcggaaac tagttcttgt tctataccta agtattgctt
    53161 actacctgct tgtccatttc ctgtagaaac catttctggc tctggaatct ctgctcctga
    53221 ttgcagcaga tcttcgatat ctgatttcaa gacatctgct tttggtccat aaactgcttg
    53281 gactccattg tctttgacga tcaatcccat cgcccctgct cgtttccagg cttcttcagt
    53341 ccctactttt tctcgatcat ttacacttac gcgtaaccga gtcatacaag catcgacatc
    53401 tttgatattt tcttttcctc ccagcaaatg aatgatctgg accacttgct gatctgctgt
    53461 tcctgttccg ctgttagata caacggtttc ttcactgtca ttgtcgtaat ttccgttacg
    53521 tcccggtgta gcaaagttga attttttgat caagaaattt gctaagaaat acgtaacaac
    53581 accaaaagcg atcacacaca acacgaagtt gataagatct ccacctagac ccgctttgat
    53641 agctaacggt gtccgagtca gcaattcgat attcccaaat gaatggaccc gcaatggcaa
    53701 gatatctgcc atcgcaaatg cagccccttg gatcactgaa taaatgacat ataatggcac
    53761 agcagcaaac ataaacatga actccaatgg ttctgttaca cctgttagaa agacagccaa
    53821 cgctgctgaa aaatacattg atttatatct tgctttctta tcaggatcga cattacgata
    53881 catcgcaaaa gccattccca tcaagatacc tgaagaacca atcatttgtc ctactttgaa
    53941 acgagccggc gtccagttat gcaacacaaa ctcatattgc gacatatccc ctgcaccttt
    54001 aagattcact aaatcagtgg cccaagccaa ccataacgga tcttgcccaa atacttgtgt
    54061 ccccgcctgt gcgcctgata aaatctcata cgatccgcca agctgtgtat agttgatagg
    54121 aatcgtcagc atatgatgca atccaaatgg caatagtaaa cgttctaatg ttccaaataa
    54181 aaacggtgct agaatcggtg cgctttcttg tgattctgca atccataaac caaaattatt
    54241 gattccagcc tgtacgtttg gccacaccac agcaagaatc aatgccacga ttgttgacca
    54301 taagataaca acaaacggaa caaagcgctt tccattgaaa aatgacaaag catccggcaa
    54361 ttttctataa ttatagtact tattataagc cattgccccg acaaatccag cgatgatccc
    54421 tacgaatact cccatattca aagctggtgc ttccaataca cttgtaaaaa atcctttaac
    54481 catgatcttc gtaccgaaca gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact
    54541 tgtgacacca aagatagccc cagtgatccg attgattaaa ataaatgaga tcccagctgc
    54601 aaatgctccg cctgctcttt cttttgccca acttcctcct attgctaaag caaataaaat
    54661 atgaagatta ccaataattg cccaaccaat attttcaatt actccgcccg ttgtgacaag
    54721 cggcgtccaa tcaggattga tcaagggaat tgatttccct atactgatca tcaacccagc
    54781 tgccggcata actgctacca caaccattaa agcctttcca aatttttgcc aaaactcaaa
    54841 actaaatatc tttttcattc cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt
    54901 atagcgtgcg ttttcttttt tgtaaatgct ttatttttta tttttctgtc tataccattc
    54961 tataaaacct ataatacgtg tgttcccttc tataattttc tcctaaaaat ttattttttt
    55021 aagctttttt ctatgaaata ttttcttttg tctattgact tgtgcaatcg gttgcgttta
    55081 ttatgaacgt ataaaacagc tcattaggag gagaaaaatg aaacagatca aacgactatt
    55141 tgctattgat ccatggaaaa tcagcacgac acaattagat aaaaaaaatc ttcgactgca
    55201 agaatcttta acaagtattg gaaacggtta tatggggatg cgcgggaact ttgaagaaac
    55261 ttactcaggg gaccaccatc aaggtactta tttggcgggt gtctggtatc ccgataagac
    55321 acgtgtcggc tggtggaaaa atggttatcc agaatacttt ggaaaagtga tcaacgcctt
    55381 gaactttatc gcgatagata tttcgatcaa cgatcagctg atcgacttgg cttctttgga
    55441 accagaagac ttttactggg aattagatat gaaaaatggt gtattatctc gtacattcac
    55501 aatcactact tctactaata aagtacgcat ttccttcgaa cgttttctga gcattgtcaa
    55561 aaaagaatca gcttatatct atttaactat agaaatgctt gaaggtagcg gaacagtcaa
    55621 actcgtatca aaattggatg gaaatgttca aaacgaagac agcaactatg atgaacattt
    55681 ttggaatgag attgatcgtg gaactataga agaaatcggc tatttgacga ctaaaactat
    55741 tccaaacaac tttgggattg aacaatttac tgttactgca gcaatgcgcc atttcgttaa
    55801 tggaaaatca gtcatccctg actatcacga agatacttta gcattacatg gttgcttgtc
    55861 tgcagaatta tctcaaggtg agacatggtc agtggaaaaa gaaattatcg ttcttactag
    55921 tagagatatt ccagaagcgc agcaagcaga aaaagctgtc tcagaattaa cgaaccagac
    55981 aaacaattat atccaagcaa aagaagagca aacagcggct tgggcagaac gatggaagct
    56041 tgccgatgtg gtcatcgaag gagacgaaga agcacaacaa ggtattcgat tcaatttgtt
    56101 ccaactattc tctacttact acggcgaaga tgaacgattg aatatcggcc ccaaaggatt
    56161 cacaggagaa aaatacggcg gagccactta ctgggataca gaagcgtatg ctgtccctct
    56221 ttatcttgct ttagccaaac ctgaagtaac taagaatctg ctgaaatatc ggcataatca
    56281 actgccgcaa gcaatccaca acgctcagca acaaggatta aaaggcgcgc tttatcctat
    56341 ggtcaccttc actggtgtcg aatgccataa cgagtgggaa atcacattcg aggagattca
    56401 ccgaaatggc gcgattgcct atgcaatcta caattatacg aattacaccg gcgatacgga
    56461 atatttgaaa aatgaaggac tggaagtttt agcagaaatc gcacgcttct gggcagaccg
    56521 tgtccatttt tcaaaacgag caaataaata catgatccac ggtgtgaccg gcccaaacga
    56581 atatgagaac aatatcaata acaattggta tacaaatacg atcgcagctt gggtcttgcg
    56641 ttacacgcga gaaaattatt taacttatca aaaagaagct gctatcacta tcagcgaaga
    56701 agaactagcc aaatggaaag atatcgcaga gaacatgtac ttcccagttg acgaagaatt
    56761 aggaatcttt gtccagcatg acacattctt agataaagat ctcatgcctg tatccgatat
    56821 tcccacaagc gatcttcctc tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg
    56881 cttcatcaag caggcagatg tactacaggg gatttatttc ttcaatgata cttttacaaa
    56941 agaagaaaag caaaaaaact ttgatttcta tgaaccaatg actgtccatg agtcttccct
    57001 ttcgccaagt atccattcga ttttagcagc agaattagga atggaaagca aagctgtaga
    57061 aatgtaccag cgaacagctc gcttggattt ggataattac aataacgaca cagaagacgg
    57121 cttgcacatc acttccatga ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat
    57181 gaaaactgcc catgatcagt tgagattcgc accatttctg ccaaaaactt ggaaagctta
    57241 ttctttccat atcaattatc gcggacgttt attgttcatc tcagtcacag atgaacaagt
    57301 tactttcgag ttattagaag gttctcctct agaaatttac gtctatgaac agtcttattc
    57361 tttagcggat cgtctagaaa taacgctaag aaaggaggaa gcaaatgttt aaaggggtat
    57421 tgtttgattt agacggtgtc attaccgata ctgctgaatt ccattatcgt gcttggcgaa
    57481 agcttggtca ggaaatcggc attacgatcg accacacatt caacgaacag ctaaaaggcg
    57541 tcagccgaga agactcactc tctttgcttt tagcttatgg cggaaaagaa cactcttttt
    57601 caaaggaaga atttgcagaa ttagcgaaaa gaaaaaacga ttattattta gagatgatcc
    57661 agacgatcga accaaaagat gtcttccccg gggtcgtgcc tttactggat tctttaaaag
    57721 aagaaaaaat caagattgct cttgcttctg caagtaaaaa cgggccattc ctacttgaaa
    57781 aaatgggtct tacaccttac tttgatgcta ttgccgaccc ggcagaagca acaaacggaa
    57841 aacctgcgcc agatatcttc ctccttgcag caaaagctgt cggactaaaa gcagaagaat
    57901 gtatcgggat cgaagacgcg caagccggta tccaagccat cctctcaagt ggcgctcaac
    57961 caattggcgt gggacgtgct gaagaccttg gagaggagat cccgcttgtc cctgatacga
    58021 cttttcttac gatcgattac ttgaaagaaa agtggcatga ccatggataa tccagagatc
    58081 tccgtcgtac cagttccaga gactgaaata aagaaaatca cattgaagaa cgactactta
    58141 gaagtcgttc ttctcaatta cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa
    58201 ggaaaatcag aaaatgtttt attgtcttac gatcgtttcg aagatgttct aacagacaaa
    58261 agcttttttg gagcaactgt cggacctgta gccggaagga tccgggatgc ttcttggggt
    58321 gagcatagat tggagaaaaa ctgcggatca caccatatcc acggtggcac gaaaggctgg
    58381 tctttccaat tttgggaagt agaagttttc aaaactccat catcaatagg tgtggtcttt
    58441 tacttaaaag atgaattttc tacctatcca ggaccaatca ctgctacgat cacttaccgt
    58501 ttaacaaaaa acgaattaga gatgacaacc tctgctagta gccttgttga aaccatctgc
    58561 aatccgacca accatgccta cttcaacctt tcaggaaatg gaacaagaga catctatgaa
    58621 catcagttaa cggtcttttt agatggtgtg cttgaattag atcaagaaaa actgcctact
    58681 ggaaattgga caaaaaaaga aaaactgcca atcgacttta gaaaatcccc aacactgcaa
    58741 gagatccttg catgttatcc agatggacta gatgatgtat ttcttttgca tcatcctagg
    58801 ctttccaaaa cttccttaca attattcgag aagcattctg gtcgccaaat gactatagca
    58861 acttctaata aaagtatggt attattttct acgactggat tcgaagctga tttctctgtc
    58921 aatggaaaac agatgcattc aaactacgga ttagcaatcg aaccgcaaga aatccctgat
    58981 atcgttcatt tccccaaatt tggatcgatc aacctccatc ctggtcaaga acggatcagt
    59041 cagacgattt atcggttctc tgcacaataa caagagaaag gtgtttttat catggctatt
    59101 accgtaaaag atgtggcgaa aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc
    59161 aacgatcatc ctagtatttc cgagacaaca aagaagaaag tccgaaaaat tatggatgaa
    59221 ctaggttatg ttcctaacat gactgcccgc aatctaggaa aacgcatttc cagtgccatc
    59281 ggcgtaatct taccccctct tgattcaaaa gagcggatcg gtaacccatt ttatcttgag
    59341 atcatggaag caatcaacga agaagcgcgg aattatgata tgactacagc aatcgctact
    59401 gctaaaaatt ttgaattgct gttagagaac gtccagcgta tgcatttaca aaaacaagta
    59461 gatggtttta ttctagtgta ttcagatagt caagatactg tcattgatta tttataccaa
    59521 aaccacatcc ctttcacttt gatcggacag ccttatcatc atgagaacga aatcgtttat
    59581 gtagataatg ataaccagct tctaggtaaa caagcaacag aatttttgat ccagaatgga
    59641 cataagaata tcctgtttgt gacaaacacg actcatgaaa gtttgtattt cgaacgatat
    59701 ttcggttatc aaaaagcaat gatgatggct ggtcttcctg cgttatccag tgtagtacta
    59761 gagcatccgg aggattatct gtcctttgag aaaacgttga aagaaaacga agccacagca
    59821 ttggtggtca ttgacgatat cttcgccctt cgcacgatgc agttggcgca aatgtatggc
    59881 tatgtcattc cagagacact ctccgtcatt agcttcaaca actcgatctt ctcaacatta
    59941 acacaccctt accttacaag tatcgatatc gatatctccg aattaggacg tgtcgctatg
    60001 caaaagctgc gcgaattgat ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct
    60061 catcgcttga tcaaaagaga gacagtcgtt cctttagaaa acccatacta aatagctgac
    60121 tcactaaata cgctgaccat ttatcctatg gtcagctttt tatgtaaaag aagatactac
    60181 ttctaacaca ttgtttatct gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt
    60241 ctcacgctct aaacacgtat aaacggcgga agcagaagca actccttcgg aaataagctg
    60301 aaattcacaa aaatttaaaa agcaattttc ttgagtttct tcttatttct tggagttaaa
    60361 cgcttctgtc ccagcctctt ttttccttcc ttcaattttt tacacgtcga cgattcaata
    60421 aaaaaccgcc taatattgta aagacaggtg caagaatcga gaaaaaggcg aatggcaagt
    60481 aatgcaacgc gctaactttc atcgttccca taatgaatgt gccgctgact ccccacggta
    60541 cgagagaatt gacggtcgca cctgaatccg ctagtgttcg tgtcaaatag gtttttgata
    60601 agccaacgtc atcaaaagaa cgcttgaatg tttctcctgg aagaatgatg gacagatact
    60661 gttctccaac gatcagattg attccgatac agctcaaggc agtaaacccg actaaacgag
    60721 aaggtcggtc catcttttct tttaattctt gaacgatggt atcaacgatc agatatttga
    60781 tcagtaaccc acctaatgcc aatgcaagta aaatcaaact agcagaacct aacatactca
    60841 tgatcccgcc tcgagaaagc agttcatcta gctttggatc aggtgtattt gcgacataac
    60901 cattcatcaa gatgtctgct gcttttccga ccgtcaaact tttctcatgg ataaaagaaa
    60961 gaacgagagc agctgtcgat cccgctagta atgtagggat agccggaatt tttttgattg
    61021 cccccccaaa taaaataact agtggaatca atgtccaagg agagatccaa aagctcgctt
    61081 tcatgttttg gatcatttca ttgatactag ctagatcggc tgaatgactg ttatgtccta
    61141 atatcgtata aaaaatcaac gaaaggacga atgctgggaa atccgtataa cgcataccag
    61201 cgatatgttc gaatagatta actcctccaa tacccgctgc caagttggtc gtatctgata
    61261 aaggcgagca gttattccct aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat
    61321 cattgaatcc aagtaaatga ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg
    61381 taaatgaact gccgacagtc actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa
    61441 agaaacgcac agaaacaagt ttaaatccat aaaccataat cgtagggatg gttccagata
    61501 aaatccatga agcaactaag acaccaatca aaagaaaaat caggattgga atgatccctg
    61561 gagtgattcc tttgatgatc ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc
    61621 cataaaacaa caaacacatg aaagctgcaa ggatcgggat atgtggcgag agctggaatc
    61681 cgatgatcag tactcctagg agtgccaaca acacgatcaa tacagcgata ctttctttta
    61741 aagaaatatt tttattcatc gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa
    61801 aaactattca cttggcagga cctcctacta aatcagcgac aaaacgtcgg ctgaaagact
    61861 tattttttta tttttcttct aaactttttt ctacctcaat cccccaagaa tctttcttga
    61921 agtgattgat gatcaatgga ataacgaaca tgaccactgt gatagctgct actaataatg
    61981 catcactcaa tgtcgctgta cctaatgtag caaatacgac aacgatgata gagaagatca
    62041 gcatgcagct gactacccaa gctaatccgt ttccgttttt accaattcgg taaggacgtt
    62101 cgaattctgg atgtttctta cgcattttaa gcaatgaaac agcaatcaaa atgtacacga
    62161 tacaataaat aacagttgta gcatttgtaa gtgtcaagaa tacagcactt acgtcatcca
    62221 ttacaccata aagcaaagca aataatgaaa taacgattga ttgtgttaga acgacatttc
    62281 tagatacgcc gtatttgttt tctttatgga atccgaattt tggtggaagg aatccttcac
    62341 gagctacttg gatgattgtt tttgatggtc cagtaaccca agctgataat tgtaaaagca
    62401 caccaataaa taccatgaag ctgaagatat ttccgatcac ttctggtaag cctaggattt
    62461 gacagtcaat caagataggc tgcgtgatat ttgctaattc catttttcca tctggtacga
    62521 catttgctac aagcatcgca ttgatgacat tcaataaaac taatccgatc aaagcgataa
    62581 atacgccttt tgtataattt tttgttgcgt ctttcaaacg aggcatgtaa acagaactca
    62641 tttcgatccc aacaaaaata aatgtgatac cagctaagta tttcaatgaa tcaatatgtt
    62701 ccaagtcagg gaaagctttt gaccatgaaa agtcacctag atagccgttt gagactagtc
    62761 cgactttgaa ggctgccaaa acacctaaaa cgaacatgat gactactggg atatataccc
    62821 ctagccatac accgatgttc ccgccgactt ttgccatatc gaatttcaag ttcaagatcg
    62881 tgatgatcca gtagataact aaaatacaac ctaaaacgaa ccagtgatta ttgccaagtt
    62941 ctacattacc aaatgtgttt cctaacaatg gtcctaaagt agaagcgacc attaccatac
    63001 ctgggaacat ttgcacccat aatagccatg caacgacaaa tccccatttg ctgcccagtg
    63061 ctgtttttac ccataactga ggaccgcctt cttgaggaag catcgtggaa agctcaccgg
    63121 aaaccattga tatcggaccg gcaaaaaaca aaactgcaaa aattgaataa aaaatcaatg
    63181 tccaaccgac tgcggctaac gtaggaatac tccgaaccgt tgcacacagt gccattgtca
    63241 gaccgataaa ggtcccaaga cccattttac ttacttcaga tttactcacc gttgacactc
    63301 tccttgattt tttggataaa ataacggtgt ctttgaagta gtttcagcaa taaacgaaac
    63361 aattcagcga atatttgagg ggattctttg agtttattgc ttgaagggtt tcttcaaaga
    63421 caacctcaaa acaaagggat cgaagagctt aggtctcccc ttgctcttca gatccaaaca
    63481 tgcgtcagaa acagttagta ttcaagctgt tttctaacat gattaccttg cttattttgc
    63541 ttcgcttgcc aataattggt ctgcgtagat tttttccaat ttttcttgaa gagctgcttt
    63601 gattttttca gcatattctt caaagtgttc ttctttgttc atgtatggtg tcattacgct
    63661 ggcacgcaat acagttactt ttccggcacg gttccattct tcatctgaga atcctagttg
    63721 gttcacaaat tgtaatgggc tgttgccata gtctgggata gcaaagtcag tatgtgaagt
    63781 taagaactca ttaccgtaaa tgcttccttt cacgtatgaa gaataatcat atacgtcatg
    63841 gttcaattta ttcattgcaa ctaagtcatc gttgcctttt tctttgaata catagtctac
    63901 catattgaaa tctggataag ttaatggatg aacttcgatt tctttgtctc ctactttaaa
    63961 tgacaagtca ttcaagaagt tgtagaaacg atgtgcacct tcgattgatg cacccattaa
    64021 tttaccatat cctgtgacat tcaaaggtaa gacatggtgt gctgcccaaa cgctggctgc
    64081 tgttgcccct gcttttgaac cttccaagat ataagcccca agtaatgctg ggatatctgc
    64141 gcctttttca aatacatatg tcgcaaagta agagataacg tctctcatgc ggatatcttt
    64201 gatgacgata ccaccggcag aatatggaac atagcccatt ttatgtgggt caatcgttac
    64261 agattctgct tcttcgattg ctttatatgc actgtgtact tcttctaaga tataatcttt
    64321 gttttctgta aagacattgt atttgtaatg aacatctttt aaatcttcaa atggaatgaa
    64381 gttgttgtct tcgtctaaga aaatcgcacg gccgtatccg ccataagcag catctacgtg
    64441 taagtagaag taaataccgt ctttttctaa tacgcgacgc aatgcaacga ttttatcgat
    64501 accgtcgatt gctccttctt ctgttgatcc tacaacaccg actacaccta ggattggtgt
    64561 tttttcagct gctagaccac gaacgatttt ttcaagttcg ttgatgtcca tgcgatagtt
    64621 gtggtcaact ggcactggaa tcacttggtc taaaccgata ccgatgatgt cggcagcttt
    64681 tagccatgaa tagtgtttag tttgtggaac caaccatttt cctaattttt ctaaatgttt
    64741 tccgctacgt gctgagtgag ctttgatttc gtcgattttt tctgggacgc tgtctaacag
    64801 attcatgatt tcttctgttg acaagttcat taattcccaa tcgctttttc cagcaactag
    64861 ttcaggtgtc acttctttca ttgcaagcgg caatgatttg atgttgcggg cataccaaag
    64921 tccttctaag tttgctaaag aaccatcagc aacgatgtgg ccccagccat ctttatagct
    64981 cattaatttt gcaaattcca ttcctacttc ttcttccatt tggcttgttg caggagaaga
    65041 ttcataggca acgttattac cattccatag catagcaaaa ttgtacgcta gtaaagatgg
    65101 catcaatgtt tcagagttca tatgacccca gtaacgtccg gcattatgcc aaggaacgga
    65161 atgggtacgc atccgcgctg aaatttctga tagaacatcc tttgttttat tcacagtgtg
    65221 ttcaaaacta gcactgcttt tttcttctgg cgtaataata ggcatgtctt gaggcatgta
    65281 gttttgacgc cagcctaaat gttcatccac caattcattc aacaaagctt tatagatttg
    65341 gccgttctca gctttgtccc caatgaatag ggcatttagg tttagatctt tcgacaatga
    65401 ttcactcata gtgaaaccct cctaaaaaaa tatttaaatt ttgtcgagcc ctacattaca
    65461 cctgttcttc tattctgtaa actaagtttt ggcctgattt ttaatcatat tttaatatgt
    65521 atcaattatc cgaattgttc acacttttga aataattaaa aaaaacgagg gaatttacaa
    65581 atgtaaactc cctcgcaaag attgtattaa cgacaaccag cgttctattt taggatgggt
    65641 ttcaaaattg gaatgttgta atagtatttc cctttttcaa ggtaaaaact tactaaattc
    65701 taatcaatta ctttggccaa aaagtaattt ttcttacctt ttcgaataac cacaaactta
    65761 ccatcgaatt catcggatgg atttatgaca aaatttaaat cggtaacgcg gtcaccgtta
    65821 atacttatag ctccgtttga aacatcttca cgtgcttgac gtttagatgg ttcaattttt
    65881 gtggaaacaa gcagttccac gatattttca ggtgtgcttg aaatttcaac gttcggcatt
    65941 ttaccgaatc cttgcgcgat ttcttccgca tttagttctt taatatttcc agagaacaat
    66001 gcttgcgtta ttttttgcgc ttctttcaag tcttcttcac tatgcacaaa tcttgtaact
    66061 tcttctgcca aacggcgttg tgcttcacgt ttctcaggtt ctgtctctac ttttttcgct
    66121 aagtcttcga tttcttcttg agacaagaaa gtaaagaatt tcaagtattt cactacatcg
    66181 cgatcatctt gattcaacca gaattggtag aactcaaatg gtgaagtttt cttcggatca
    66241 agccaaacgg caccgcctgc agtttttccg aatttcgttc cgtcagcctt cagcatcaat
    66301 gggatcgtta aaccgaacgc ttctgcttct ggtccttcta attttcggat taggtctaat
    66361 ccagctgtga tatttcccca ttggtctgct ccaccgattt gcaattgcac atcgtatgtt
    66421 ttatgcagat gcaaaaagtc gatagattgc aggatttggt aagtaaactc tgtaaatgag
    66481 attcctactt ccaaacggct ggcaacgata tcttttgcta acatcgtgtt gatgttgaag
    66541 tttttaccgt agtctctcaa aaattcaagt aaagagattt ttgacaacca gtcgtagttg
    66601 ttcacaaatg tgatatttgc atctttacca aacaattttc tcatttgatt ggaaagtgcg
    66661 tccacgttat gttgcacttg ttccatcgtt tgcaacacac gttctgtttt gcggccgctt
    66721 gggtcgccga tcgttcctgt tccgccgcca atcagaatat atggatgatg gcctgccaat
    66781 tggaatcttt tcatcatcat aaatggaatc aaatgtccga tatgcatcga atcaccagtt
    66841 ggatcaacgc cgcaataaag tgaaatgctc ttttcttcaa cgagctcgcg aagtctttct
    66901 tcgtttgttt gctgattaat ggcgtcgcgc caagttaatt catcaataat attcatttta
    66961 ctgacctccg tgtagttttt ttatgaaagc aggaacctgc tagcatgtgt aattaattat
    67021 ttcttcagga taggatagga aataaaaaaa gtcccttggc atataagcca agggacgaaa
    67081 tctcgcggta ccacccaatt tgtatgtcta gatatccaac atacctctcg aacatttaac
    67141 gctgtcgcgt ggcaatcact gccaaagact cctgaaatgt aattcgcttt aaagcatgta
    67201 ctggtttgca gcgaccacca gctttctgaa acattggctt taacaactac ttcgtttcga
    67261 tcttcgtctt gttattatta atatgtgtct atcatagagc gcaacttttt ctttgtcaac
    67321 agaaaaagga tatttttttt aaattcctca ttttttcttt tttgaatccg ctctaattcc
    67381 gaacatttta ttttcaatta ttgttaaaag aaataacttt ttctgtattt tttctgcaat
    67441 ttatcgagaa gtcactttac tttgcctctt attctttctt cgttacaata aggaatggga
    67501 caagtaaaaa aatgaaaaag caagggacga ggtatatgga aaaaaagcaa aaaattattt
    67561 taggagccac ttctgcttta actatcgtta ttttaattct aagtttgttt tttgtcatcc
    67621 gatcatctac aaaaaaagaa ccaatcattc ctgaaaaaaa aataacagaa aaagcaaaag
    67681 aaccgacaga tgataagcaa caactagtta ctcaagcaaa aaatctggct caaagctacg
    67741 attatgacca agcgattgcg ttattggaaa aagatagtaa gaacggatct actgaaacac
    67801 aacagcttct tcaaacacta aaaaaggaga aagaacaact gatcgactgg tctgatccta
    67861 ctcagatctc tcatgtcttt ttccacagct tgatcgtgga cccgcaaaaa gccttccact
    67921 ctcaacaggc acaaggatac aaagattata tggtgactgt tgaagaattc aaccgttcga
    67981 tcgaacaact ttatcaaaat gattttgttt tagttaattt gaatgatttg atccaaaaag
    68041 acgaacaagg aaatctttcc tttaacgggc ttgcgctacc tgaaggaaaa aaaccattgg
    68101 ttctttcaca agatgatgtc agctattatg aatatatgga taattccggt tttcctagcc
    68161 gattggtcgt atcaaataac aatgaaatca aaaatgtcta taaagagaaa gataaagaag
    68221 aaatcggtga ctttgacatg gttcctctga tcgatgcctt tgtcaagaaa catcctgatt
    68281 tttcttatcg aggcgcaaaa ggtaccttgg ccctgacagg ttacaacgga gttttaggtt
    68341 atcgaacatc aaaatctcaa tatggagaca atgaaaaaac aaaaaaagag attaaagaag
    68401 ctaaaaaagt ggcggaacag ttgaagaaag acggctggag ctttgcttcc catacatggg
    68461 gtcacttgaa tatgactcaa gcttcactag aagatgtaaa aaaagacaat gaattgtggc
    68521 agcaagaagt tgcacctatc ctaggaaaaa caaatattct gatttatcca tttggtgcag
    68581 acatcagcga ctggcagcct tattcagcag aaaatcaaaa atttgcttac ttgaagagtc
    68641 aaggtttcga tattttctgt aatgtcgatg cttctacacc agcttgggga caattaggta
    68701 cggatttcta tcgaaatgct cggatcaata tcgatggcat tcgttttcaa tctgatttag
    68761 acggaaaaaa tcctattctt gatccattca tcaatgtcca tgatgtctat gacaaagaag
    68821 cacgcgaatc tgcttgattt tacgtcattt atgaatcgag tagaggggaa agccaactga
    68881 ccttccgccc ctctcacacc accgtacgta cggacccgta tacggcggtt caataagttg
    68941 agtgtacgaa ctggtacttc aaggagatgt cctcatatcc ttggcgggcc agttctttgt
    69001 ctgttaatga tcgatgaaga atatgacttt tggagactga ccaaatacct tttcttgtat
    69061 ttgaccattc atacgctttc gctttatcga tccctaaacg aatcagattt tttcttttgg
    69121 ttcttggatt cttccattgt ttccaaatat attgtctgat tcttcgcttc aaccaaccgt
    69181 ttaactccct catgaattct ttcatctcac caattccata gtagttgatc caaccagtca
    69241 ttagttgctt gatttctttt aaaataagtt ggatacttct tccacggttt cttttagtga
    69301 gttgtttgag cttgcttttc acacgctgtt ttgcttttgc gtgagggcga attttaatac
    69361 ctttctttgt tggcagaatg cagaatccaa gaaactttcg tttcaatgga cttcctaccg
    69421 cactcttttc tcgatttacc gtcaacttta gttgttcttc gagaaattga atggatttct
    69481 tcatcacacg ctgtcctgct cgtttgcttc taacatagat attgcagtca tcggcgtatc
    69541 gaacaaattt atgtcctaga ttttctagca cttgatcaaa ttcgttcagg tagatgttac
    69601 ttaacagggg agagagattg cctccttggg gcgttccttc ttcagttgtt gtgatgagtc
    69661 cattttccat tacaccactc attaagtatt gacggatcag ttttaggact tgtttgtcct
    69721 ttactttccg ctcaatgaaa tacatcagct tatcgtgatt tacggtgtca aagtaggctt
    69781 tcatatcaat gtccaccaca tatcggtaac cttgttcata ataagatttt gctttcttca
    69841 ctgcgtcatg tgcgctgcga tttgggcgaa acccataact attatctgag aattctggtt
    69901 caaatagcgg ttgtaacact tgattgattg cctgttgaag catacgatca ataaccgtgg
    69961 gaattcctag ttttcttaca ccgccgtttg gtttcggtat ttctactcgc aaaacaggct
    70021 gaggtttata gttcccttgg cgtaaattgg atagtaattc ctcgcggtgc tctttcaagt
    70081 aaggaagcag ttgatcgacg gtcatcccat ctgcaccagc cgccccttta tttctgacta
    70141 cttgaagata cgcttgattg agattattcc gatctagaat tctcccaaac agattttcta
    70201 caccatcttg tttcctctgt tcaccgcaag aagtgctgcg cacttcatca tacccttcgt
    70261 gttccacact agctctttga tgatagccaa gttttcttgt tttctgcggt cgtcgcatct
    70321 tctcacacct cctaaattat tcaagattct tattgttcag tccttcgtaa cggtcagtgt
    70381 cgttactact atgacctcgg ctgacttctc atgattcacc atcatgtcgc catgatcgtt
    70441 gtgctggttt gttttcattc aacgcccaca acaccctcat gagatctccc ccggtaagag
    70501 tgaaaacttt cctcccatgt aactgctaga tttactgtat aggtttcggg cagtattgga
    70561 ctttgtcttg tgatgaagac ttatccgacc taattcagcc ttgtatctag tttctgttcg
    70621 tcagttcagg agtttgcgtc cgacttcctt cagccacaac ctcacgattg ccgccttgtc
    70681 tttcgctaac agttcctact gccaagcctg tagtggactt tcaccaccaa gttttcaccc
    70741 atgcagggcg cacaccaaaa aagccgaact gacattttat cgtcagtttc ggctttttta
    70801 tgctttattt cttcttcggt gcgattccct tgatcaggac ttctgtccat tctgctgtat
    70861 atggaagcag ttttgcgtat atctccggct tagaaattcc ttctaccatt gcttgaaaat
    70921 agaggatcaa gaactcatcc gaatatttca aatccacttt tccttctttc cgccctcgat
    70981 ggaaaagatc aagcatgata ctgaaacttt cttgcgtata cgcatccatc gcgtcagaaa
    71041 attctttttt ttcttttttt gtatagtatg tcatcaaatc gatataaaat tgtttgttca
    71101 ctcgattgag ataacggatt ttgttttcac tcatggcaat cagtgtttct tcaaatggaa
    71161 gattttccgc aatcaacgct ttcgcttctt gccctaaatc attcataaat gcaaaaaata
    71221 cttcataaat cagcccttcc ttgctgccaa aatatttaaa aatcgtcgct ttacttacct
    71281 gagcgttctc agctacctta tccattgtca ctccagcggt tcctatgtct gtgttcatca
    71341 agtcaaaagc tgcttgtaaa atctctttct ttttctcttg tgctctcttt tcaaaaccgt
    71401 tcatttgtag agcctcccgt aattttaaac ttttataata tatatagttc aaatttattc
    71461 tgaggtttat tataactatc tattgagaaa gtatcaagct agcaattcct tgtttatgaa
    71521 ttcattgaca gaaatttat
//
LOCUS       DACEQH010000006        68910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.006, whole genome shotgun sequence.
ACCESSION   DACEQH010000006 DACEQH010000000
VERSION     DACEQH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.006"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(105..629)
                     /locus_tag="IW833_000382"
     CDS             complement(105..629)
                     /locus_tag="IW833_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1306527.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IW833_000383"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IW833_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ1306528.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IW833_000384"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IW833_000384"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ1306529.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="IW833_000385"
     CDS             complement(2351..3031)
                     /locus_tag="IW833_000385"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1306530.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="IW833_000386"
     CDS             3157..4026
                     /locus_tag="IW833_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1306531.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="IW833_000387"
     CDS             4052..4567
                     /locus_tag="IW833_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306532.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="IW833_000388"
     CDS             complement(4614..5177)
                     /locus_tag="IW833_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1306533.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="IW833_000389"
     CDS             complement(5232..5411)
                     /locus_tag="IW833_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306534.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="IW833_000390"
     CDS             complement(5488..5997)
                     /locus_tag="IW833_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306535.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="IW833_000391"
     CDS             complement(6093..6473)
                     /locus_tag="IW833_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1306536.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="IW833_000392"
     CDS             6803..7552
                     /locus_tag="IW833_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ1306537.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="IW833_000393"
     CDS             complement(7736..9013)
                     /locus_tag="IW833_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306538.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IW833_000394"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IW833_000394"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ1306539.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVSFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="IW833_000395"
     CDS             complement(9935..10879)
                     /locus_tag="IW833_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ1306540.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="IW833_000396"
     CDS             complement(10881..11567)
                     /locus_tag="IW833_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ1306541.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="IW833_000397"
     CDS             12041..12361
                     /locus_tag="IW833_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ1306542.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="IW833_000398"
     CDS             12515..13420
                     /locus_tag="IW833_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1306543.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="IW833_000399"
     CDS             13417..14190
                     /locus_tag="IW833_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1306544.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="IW833_000400"
     CDS             complement(14371..15795)
                     /locus_tag="IW833_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1306545.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16006..16452
                     /gene="nrdI"
                     /locus_tag="IW833_000401"
     CDS             16006..16452
                     /gene="nrdI"
                     /locus_tag="IW833_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1306546.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16517..18499)
                     /locus_tag="IW833_000402"
     CDS             complement(16517..18499)
                     /locus_tag="IW833_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1306547.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IW833_000403"
     CDS             complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IW833_000403"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1306548.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19412..20473)
                     /locus_tag="IW833_000404"
     CDS             complement(19412..20473)
                     /locus_tag="IW833_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1306549.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20684..21211)
                     /locus_tag="IW833_000405"
     CDS             complement(20684..21211)
                     /locus_tag="IW833_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1306550.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21278..22780)
                     /locus_tag="IW833_000406"
     CDS             complement(21278..22780)
                     /locus_tag="IW833_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1306551.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23038..24159)
                     /locus_tag="IW833_000407"
     CDS             complement(23038..24159)
                     /locus_tag="IW833_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1306552.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24285..25226
                     /locus_tag="IW833_000408"
     CDS             24285..25226
                     /locus_tag="IW833_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1306553.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25376..26272)
                     /locus_tag="IW833_000409"
     CDS             complement(25376..26272)
                     /locus_tag="IW833_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1306554.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26541..27881
                     /locus_tag="IW833_000410"
     CDS             26541..27881
                     /locus_tag="IW833_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1306555.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28099..28542
                     /locus_tag="IW833_000411"
     CDS             28099..28542
                     /locus_tag="IW833_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1306556.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="IW833_000412"
     CDS             complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="IW833_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1306557.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30867..32090)
                     /locus_tag="IW833_000413"
     CDS             complement(30867..32090)
                     /locus_tag="IW833_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ1306558.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32121..32960)
                     /locus_tag="IW833_000414"
     CDS             complement(32121..32960)
                     /locus_tag="IW833_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ1306559.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32977..33657)
                     /locus_tag="IW833_000415"
     CDS             complement(32977..33657)
                     /locus_tag="IW833_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1306560.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33659..34732)
                     /locus_tag="IW833_000416"
     CDS             complement(33659..34732)
                     /locus_tag="IW833_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1306561.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(35379..36050)
                     /locus_tag="IW833_000417"
     CDS             complement(35379..36050)
                     /locus_tag="IW833_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1306562.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="IW833_000418"
     CDS             complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="IW833_000418"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ1306563.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            39474..39911
                     /gene="ndk"
                     /locus_tag="IW833_000419"
     CDS             39474..39911
                     /gene="ndk"
                     /locus_tag="IW833_000419"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ1306564.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39933..40799)
                     /locus_tag="IW833_000420"
     CDS             complement(39933..40799)
                     /locus_tag="IW833_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1306565.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40959..43052)
                     /locus_tag="IW833_000421"
     CDS             complement(40959..43052)
                     /locus_tag="IW833_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1306566.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(43801..44409)
                     /locus_tag="IW833_000422"
     CDS             complement(43801..44409)
                     /locus_tag="IW833_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306567.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLITAYSEKVDKKVVADRLAEWKKTNEQVKKTTVSSQK
                     NESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(44568..45965)
                     /locus_tag="IW833_000423"
     CDS             complement(44568..45965)
                     /locus_tag="IW833_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1306568.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(45997..46968)
                     /locus_tag="IW833_000424"
     CDS             complement(45997..46968)
                     /locus_tag="IW833_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1306569.1"
                     /translation="MESIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            47342..47767
                     /locus_tag="IW833_000425"
     CDS             47342..47767
                     /locus_tag="IW833_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306570.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(48255..48956)
                     /locus_tag="IW833_000426"
     CDS             complement(48255..48956)
                     /locus_tag="IW833_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1306571.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(48972..49412)
                     /locus_tag="IW833_000427"
     CDS             complement(48972..49412)
                     /locus_tag="IW833_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1306572.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(49412..49987)
                     /locus_tag="IW833_000428"
     CDS             complement(49412..49987)
                     /locus_tag="IW833_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ1306573.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(50835..51314)
                     /locus_tag="IW833_000429"
                     /pseudo
     CDS             complement(50835..51314)
                     /locus_tag="IW833_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(51473..52174)
                     /locus_tag="IW833_000430"
     CDS             complement(51473..52174)
                     /locus_tag="IW833_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1306574.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLDHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(52282..53691)
                     /locus_tag="IW833_000431"
     CDS             complement(52282..53691)
                     /locus_tag="IW833_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ1306575.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(53739..55604)
                     /locus_tag="IW833_000432"
     CDS             complement(53739..55604)
                     /locus_tag="IW833_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1306576.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAEADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHRGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(55725..56576)
                     /locus_tag="IW833_000433"
     CDS             complement(55725..56576)
                     /locus_tag="IW833_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1306577.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(56886..57599)
                     /locus_tag="IW833_000434"
     CDS             complement(56886..57599)
                     /locus_tag="IW833_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1306578.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(57667..57993)
                     /locus_tag="IW833_000435"
     CDS             complement(57667..57993)
                     /locus_tag="IW833_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1306579.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(58116..58343)
                     /locus_tag="IW833_000436"
     CDS             complement(58116..58343)
                     /locus_tag="IW833_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1306580.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(58665..59918)
                     /locus_tag="IW833_000437"
     CDS             complement(58665..59918)
                     /locus_tag="IW833_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1306581.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            59979..60719
                     /locus_tag="IW833_000438"
     CDS             59979..60719
                     /locus_tag="IW833_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1306582.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(60805..61563)
                     /locus_tag="IW833_000439"
     CDS             complement(60805..61563)
                     /locus_tag="IW833_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1306583.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(61560..62588)
                     /locus_tag="IW833_000440"
                     /pseudo
     CDS             complement(61560..62588)
                     /locus_tag="IW833_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(62608..65775)
                     /locus_tag="IW833_000441"
     CDS             complement(62608..65775)
                     /locus_tag="IW833_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306584.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(65999..66883)
                     /locus_tag="IW833_000442"
     CDS             complement(65999..66883)
                     /locus_tag="IW833_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1306585.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(66978..67415)
                     /locus_tag="IW833_000443"
     CDS             complement(66978..67415)
                     /locus_tag="IW833_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306586.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(67632..68543)
                     /locus_tag="IW833_000444"
     CDS             complement(67632..68543)
                     /locus_tag="IW833_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1306587.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT        19759 a        14472 c        11868 g        22811 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaatttaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatgaca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
    15901 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
    15961 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
    16021 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
    16081 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
    16141 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
    16201 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
    16261 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
    16321 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
    16381 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
    16441 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
    16501 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
    16561 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
    16621 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
    16681 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
    16741 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
    16801 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
    16861 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
    16921 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
    16981 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
    17041 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
    17101 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
    17161 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
    17221 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
    17281 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
    17341 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
    17401 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
    17461 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
    17521 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
    17581 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
    17641 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
    17701 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
    17761 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
    17821 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
    17881 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
    17941 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
    18001 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
    18061 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
    18121 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
    18181 cagatgctga tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca
    18241 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
    18301 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
    18361 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
    18421 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
    18481 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
    18541 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
    18601 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
    18661 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
    18721 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
    18781 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
    18841 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
    18901 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
    18961 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
    19021 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
    19081 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
    19141 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
    19201 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
    19261 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
    19321 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
    19381 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
    19441 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
    19501 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
    19561 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
    19621 ttgctgggat aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg
    19681 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
    19741 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt
    19801 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
    19861 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
    19921 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
    19981 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
    20041 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
    20101 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
    20161 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
    20221 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
    20281 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
    20341 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
    20401 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
    20461 aaaagccaaa catattatcc ctctttttct gtatccgtct tcatttctat gactagagta
    20521 tatatctaaa gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa
    20581 ggttgagttt atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa
    20641 gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca
    20701 attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag
    20761 cattgatcac tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt
    20821 cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat
    20881 ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca
    20941 gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc
    21001 tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
    21061 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct
    21121 gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag
    21181 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc
    21241 ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa
    21301 tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt
    21361 tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
    21421 gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt
    21481 tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa
    21541 gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg
    21601 ccaacgttcc tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg
    21661 gctggtattt ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg
    21721 atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa
    21781 aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa
    21841 gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt
    21901 ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc
    21961 gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag
    22021 tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg
    22081 ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag
    22141 ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    22201 aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    22261 cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt
    22321 gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    22381 catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga
    22441 attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc
    22501 atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg
    22561 tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt
    22621 tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    22681 gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt
    22741 cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa
    22801 aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa
    22861 agacccatac atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct
    22921 ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat
    22981 ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta
    23041 cttcgggagg atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa
    23101 tgtttgcctg tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac
    23161 aattgggaga aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat
    23221 tattggtttt cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat
    23281 cgtttgctga tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt
    23341 ttccaatcca gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa
    23401 aaaaagggct ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac
    23461 ctcccagaaa tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag
    23521 cgtcgtatct attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt
    23581 atatggattg gtaacatctg cagcgataat caattttact ttagaaacaa gaacgttttt
    23641 tcgatcaaaa tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa
    23701 agaagcaccc aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa
    23761 agtgacaata atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc
    23821 ataactggaa gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa
    23881 tgcttgtgca gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct
    23941 gatttttcgc tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag
    24001 cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt
    24061 tcttccttta cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc
    24121 acttccttta aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct
    24181 taagtaagac actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt
    24241 ctatatacta aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag
    24301 ctggggccgg agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg
    24361 tcctcttgat cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg
    24421 ctgactttaa cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca
    24481 tcgaaagcaa cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg
    24541 atatgttttg ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa
    24601 atggattagg tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg
    24661 ggatcacgat gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg
    24721 gagaaatcga actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag
    24781 aagtctttca agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt
    24841 ggcgaaaagc ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata
    24901 ttgctgaact aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat
    24961 ttgcttctat cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata
    25021 ttgcttctac ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc
    25081 tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag
    25141 gacaaaaata tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa
    25201 aagaaggaat ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag
    25261 ctggttctct ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc
    25321 cataagaatt gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt
    25381 tgttttcaac agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga
    25441 taaatcgttt tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct
    25501 taaatggtgt atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa
    25561 tattttcagg acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct
    25621 tttgaaaata catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca
    25681 gttccagaat cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc
    25741 caggttgttc attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta
    25801 taaattcctg cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa
    25861 atagatccgt ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat
    25921 tatgtttcag cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg
    25981 ccggttcatc ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa
    26041 aaaataacac aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa
    26101 atccataatc taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa
    26161 tgaattccga ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca
    26221 aaaaataaag tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg
    26281 cctcactttc tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa
    26341 aacgtttcct atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc
    26401 attcttctat taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg
    26461 ttatcattag tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat
    26521 ttaaaagaaa gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac
    26581 atatcaccat gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg
    26641 aagcgattgg gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt
    26701 tatatatcgt gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg
    26761 gattatctgg tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg
    26821 tctactggtc tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt
    26881 tgcagttgtg gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa
    26941 ctacgatcat caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt
    27001 ttgctaaaat cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc
    27061 aaatgttggg cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta
    27121 ctcctcatgg tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg
    27181 gttctgaact gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta
    27241 aagcaatcaa aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc
    27301 ttttgattct ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg
    27361 tatttgaaaa aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg
    27421 ctttgttctc atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt
    27481 tgaaggatcg aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc
    27541 aacgtgctgt tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg
    27601 tcggtgacaa attatttggc tatctggcag gttccctttc gtatacagta ttattgatct
    27661 ggatcattat cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata
    27721 aaggaatcag cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca
    27781 cgaactccat tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt
    27841 atcaaaaaaa gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat
    27901 tttatttatg tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg
    27961 caactccttt ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc
    28021 tctgtgcaaa ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa
    28081 aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca
    28141 acggtagtta tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc
    28201 agtcgtacac gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg
    28261 ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt
    28321 cttcttgttt ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca
    28381 gcttggatga aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct
    28441 gatttaaaag atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa
    28501 gagatcgaca aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata
    28561 aaaaaagagg atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga
    28621 acgccgtgaa taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa
    28681 caaaaatagc ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
    28741 gaacaccgtt tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa
    28801 atggaaaaat agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat
    28861 tgaacaccgt ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat
    28921 tttgtgaaat ggctttccac atttttgtat aaatttggct taataccgag aaactggctt
    28981 ttgaataccg tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta
    29041 tgtttttatt gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt
    29101 tttgagattc cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg
    29161 aaaaatacga atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt
    29221 ccggcaattc cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta
    29281 cctaatacag ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc
    29341 agaataacag ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat
    29401 gttgtaaaaa tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt
    29461 gagatcaagc caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac
    29521 acagtaggca tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt
    29581 ccagtatttg aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt
    29641 gggttttgaa taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt
    29701 gccggcacct ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt
    29761 gaaatagtaa agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg
    29821 gctttacttg tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca
    29881 gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct
    29941 agatttgttg attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata
    30001 gctccaacaa ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc
    30061 ccaatcgttg acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca
    30121 acaaatacag aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga
    30181 gttggaatga tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata
    30241 aagattggga tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc
    30301 gggtatcttt ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata
    30361 ttaggtgaga cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat
    30421 acaagtgttg cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt
    30481 ttagttgttc ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc
    30541 gcgattcctc ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc
    30601 ggtaccacct taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat
    30661 ggactttcca tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct
    30721 gaacaaaaca cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat
    30781 aagatcattt gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata
    30841 actttcacac acaagtttat tttttactag aaagtagcgc cctcttgttt ttttcttgct
    30901 actatttctt gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca
    30961 agagctgtat tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt
    31021 ttagcagcag ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc
    31081 gtaggctgga ttgttggtat gacttggctg acatttccta catcagaaga accgagactt
    31141 gttctttgtt ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag
    31201 aataagtcat caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca
    31261 aactcaaaag tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca
    31321 tatactttat ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa
    31381 tctggaatga cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct
    31441 tttggcaatt gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa
    31501 gcatttactc ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg
    31561 tcgactggat cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg
    31621 cataacgcta catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat
    31681 ccgttctcgc ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca
    31741 tcaatgaatt gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc
    31801 ccgcatgcat gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga
    31861 ccgggtttat tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt
    31921 tctacccaaa acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca
    31981 tagttactta cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa
    32041 cgatattgga ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc
    32101 tcctatgcta gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat
    32161 ccttttgatg ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt
    32221 ttttgatacg ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga
    32281 cgtgcatggt catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca
    32341 tctacagcta caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt
    32401 tgggaagcat ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca
    32461 gtgggcgctt gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga
    32521 agagctctgc cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata
    32581 tcagctacat ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg
    32641 atcgatacta aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg
    32701 aaagaattca aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta
    32761 aatttcacgt attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat
    32821 acatctgtat cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta
    32881 tctgcttttc cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca
    32941 aaacgaataa tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag
    33001 ttgattactg ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat
    33061 cgttacatct gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc
    33121 tgcacctact gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga
    33181 gacccgaatg ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg
    33241 actggttccc atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt
    33301 ctcgatttga cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt
    33361 cgcaccaatc gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat
    33421 aatgaaaggg atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg
    33481 gttagctagt aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat
    33541 catcccgcca atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac
    33601 aaattcatcc ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt
    33661 atcttcctcc ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta
    33721 tttttacacc agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag
    33781 ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata
    33841 aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct
    33901 gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag
    33961 aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt
    34021 tggtcaattc ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc
    34081 ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag
    34141 tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct
    34201 gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt
    34261 cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag
    34321 gataagcaga tgctttttct tccaaaccga caagttccag tagttcattc actttttgac
    34381 gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag
    34441 ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc
    34501 gcaattcttt gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg
    34561 ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac
    34621 cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta
    34681 tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc
    34741 ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt
    34801 ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg
    34861 atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg
    34921 aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc
    34981 tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa
    35041 gtccttttac taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt
    35101 ttcggaagtt tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta
    35161 acggtcgaac agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc
    35221 agcttttctt acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta
    35281 ctttcctttt caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg
    35341 aaaagtcaag tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt
    35401 aacatttcaa taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt
    35461 tcttcgacgg tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct
    35521 gctcgtgaag cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct
    35581 tctaatcctg ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac
    35641 acttcgtctc cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata
    35701 tctgttgctc ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt
    35761 aagtctgcca atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt
    35821 tgataaaaat attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct
    35881 gctttcacta aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt
    35941 tcttttggga tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt
    36001 ttttctgtgt caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta
    36061 atcctttcca aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa
    36121 gaaccagcct tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag
    36181 ctgtcacaag cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag
    36241 gaagtggcat caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa
    36301 taattcctaa tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta
    36361 ctttcggtgt acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc
    36421 atcctgcatg gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac
    36481 ttccgcccac tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa
    36541 atatcgaact tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt
    36601 tttttggctc gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa
    36661 catcatagat cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga
    36721 acgtacttgc aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga
    36781 tatatttcat gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg
    36841 tcaaatcttt ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat
    36901 ctacagaaat accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta
    36961 aaaatccgac agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag
    37021 gattcaattt tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca
    37081 tttccgagat tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca
    37141 ctgaacgtgt aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt
    37201 tcagagcttt caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac
    37261 caatcaaaac catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg
    37321 tcttttgtgc gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa
    37381 gtacggcttc ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt
    37441 tagaaatgtt cagcatttct tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg
    37501 gatcagcaac cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt
    37561 tttgataatt gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat
    37621 ctaacaaatt tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac
    37681 gatcatcttc atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca
    37741 atgtccccgt tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca
    37801 aatttttgat cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg
    37861 taacgatcat tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa
    37921 aagcttctag ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca
    37981 atgacataaa tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt
    38041 gaattggttt ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag
    38101 aaacaatgat tccttccgca cttccactga tgacattact tcccataaag acaagatttt
    38161 cgtaacttgt ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat
    38221 agctttcccc tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca
    38281 tatccgctgg tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt
    38341 ccataggaat ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg
    38401 atttcagttt ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc
    38461 tgacgataac catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg
    38521 ctaatacata ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg
    38581 gcgtgatgta tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg
    38641 cattttcacc gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg
    38701 atgcatggaa tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt
    38761 tttgtgtatc tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt
    38821 ctcgctgctt ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct
    38881 gagaacagat agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt
    38941 catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt
    39001 tcgaatagtt atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg
    39061 cggcaacaaa aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa
    39121 aaagcttctc agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct
    39181 gcttttagct accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc
    39241 tctcgacgtt tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat
    39301 tcggttttcc ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt
    39361 ttttcatact ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc
    39421 tcattttcca cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa
    39481 gaacacttgt tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc
    39541 aacgttttga agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg
    39601 aattagcaaa agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg
    39661 attacatgac ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata
    39721 tcgtccgtaa gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag
    39781 gagactacgc gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg
    39841 cggtaaaaga gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag
    39901 cggcgaagtg atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc
    39961 agttttttct ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag
    40021 aattcttggt agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa
    40081 taatctgcca tcaatgtttc atagatcact gtacttcttt ctttatgtgc taagctccgt
    40141 ttcatatctt cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa
    40201 gtcaggttca ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt
    40261 cgcattcttt ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa
    40321 ggaaaatcag ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat
    40381 tcttcaatga ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt
    40441 ttttcgacca tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc
    40501 ctacggttat tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc
    40561 attcgtcttt ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat
    40621 tctgcaagtg tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt
    40681 ttcactgaat ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat
    40741 tgggactgtg tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata
    40801 atagttctcc tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga
    40861 aaaatacacg aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat
    40921 tagaatgttg tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt
    40981 tcaacgtctg ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa
    41041 aagacacttg tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca
    41101 tttttatagt catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc
    41161 aagagatttt cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga
    41221 tctttggaga tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga
    41281 ttattgtaag cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga
    41341 aaagcaacta cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct
    41401 ttatcatcga ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat
    41461 tcatgattga ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc
    41521 atctgtgcgt tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa
    41581 tctttattat ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt
    41641 tggtaaatac cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa
    41701 tgtgccgttc gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg
    41761 aaattcttgt catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa
    41821 aacggctgtt gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat
    41881 atcccataac ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca
    41941 taacctaagt ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa
    42001 acagcgcttg tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg
    42061 tctgacccaa gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac
    42121 atattttcag catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg
    42181 atcgttgctt gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc
    42241 ttcatattaa tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct
    42301 ttagcgatcc caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa
    42361 acttcatcaa tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg
    42421 attccgtcat aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca
    42481 aatgttcgct gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa
    42541 ataagccctg aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg
    42601 ataggtattt tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc
    42661 cagtagatga catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac
    42721 actttcgaaa agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa
    42781 ataatattca agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac
    42841 gctggctttg gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt
    42901 gggttgatcc acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca
    42961 tagtaggcaa tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa
    43021 cgattttggc acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt
    43081 ttcttatttt gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt
    43141 ttattctttc taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag
    43201 cgattaaact aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa
    43261 aagccagttt ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct
    43321 ccttatttct tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg
    43381 ccaactcctc ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt
    43441 gttccttgtg gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat
    43501 cagctgaccc tcggtattag attatgtcag gacgctaatt ttacaacctc ttggtaaacg
    43561 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta
    43621 ttttttgaaa ttgctccttg tggctcagag ttaaacggca ggctcaagac ctcttgatac
    43681 acggtgttca atcagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggaact
    43741 attttgccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctctgtttt
    43801 tcattgtcca taggattgat attgtccttg tgatgactca ttcgtaataa ttgaggcttg
    43861 attttcttca cttcccgtta tcgtttgtcc actgccagaa tctgaaggac ttgttcctgt
    43921 cgactcgttt ttttgagaag agacagttgt tttcttgact tgttcatttg tctttttcca
    43981 ttccgctaaa cgatcggcga ccactttttt gtctactttt tcagaataag cggtgatcaa
    44041 acgggctact ttgtcgccat catgagtcaa aagcaaaacg gtcaaatgct tgtctttaaa
    44101 ttgatattgt ctgagagaaa tcgcactaag tacgtcatcg atattaataa attgattttc
    44161 ttcttttcct tttggcaaat aaatgtccaa ttcgcctttt tcgtaattgg cagttatttt
    44221 atactccgcc caccaataag ttgagaattc ttttttgaaa ttatctactg attctttgct
    44281 tgctacgatt ttttctgttt ctttctgagc aagatctgct gtcatttttc ctgaaaatgc
    44341 tgcggataaa ataagcagta agaaaaaacc gaataaccaa aagagaagaa acggtctttt
    44401 tttcctcatg ctttcctcca ctcctattta attttgacta ttctattata tacaaaaaat
    44461 gaaatagaaa gacaaaaaag ttgaaaaaga tcgtaaataa ttgaacttct ttttcacaaa
    44521 atggtaagaa tggagaattt cacgcatttg ctctcaacta ttctttctta agcctcaaat
    44581 ccgtttttat ctgatacgtc tttgaaccaa tgacccgatt ttttgattgt tctttttcca
    44641 tcttgtgcta agtctactga aataaagcca tatcgattct tatatgcatt ggtccacgac
    44701 cagttatcca tacatgtcca catatgatag cctaaacaat tggcaccttc ttgaatggct
    44761 tgatggacat atttcagatg agattggaca aattcgatcc gataatcatc gtggatcatg
    44821 ccatcttctt tgataaatcg tgcttcgttt tctacaccca taccgttttc agaaataaag
    44881 cagtcgatat ttccataatg atctcgtaca ttgatcaaaa tatcatagat tccttgttca
    44941 tagatctccc atccgcgata aggattcatt ttacgaccag gcatttcata attgtcgaaa
    45001 taatcctcag gcattggacc gtttttagta tcgattggtg tttcttttgc ttttacccgg
    45061 cgtggctggt aataattgat acctaaaaga tctactgtat tttctttgat aaccgtcaaa
    45121 tcctctgcat ggatcacagg catgagatca agttctttta ctatctcaat caattcactt
    45181 ggaaactcgc cttttacagc tggatcaagg aatgaacgat tgaagaacgc atctgctatt
    45241 tgcgctgctt taacatcttc agggtttgat tcgtctcttg gatagcttgg tgtcagattc
    45301 aagatgatac caataacacc accttgctcc atttcatggt aagatttgat ggctaacgct
    45361 gaagccaacg cttcatgata gccaacttgg atcgcttttt tcatatctga ctcttctgga
    45421 taatgccatc gatataaata acctgcttcg actggaacga ttggttcatt atgagtaaac
    45481 cattttttta ctttcgaacc aaacaattca aaacaacttt tggcatattt ttcataggct
    45541 tcaaccgttt ctcggttcaa ccatccgcct ttttcttgca gatacatcgg catatcgaaa
    45601 tgatacagat tcacaaaggg ttcgataccg ttttcaatca actcatcaaa ataacgatga
    45661 tagaaacgaa cggcttcttc gttgattgtt ccagattcat caggtatcaa acggctccat
    45721 tggatagatg ttcgaaatga attatgccca gtctgtttca tcaactgtac atcctcttga
    45781 tacttcgtat aaacttgcga ggttttttca ggtcctactt gatcaaagaa cttttgcggt
    45841 tcttttttgt accaataatc aaaaatgttc tctccttttc catcgcctga aatccttcct
    45901 tctgtttgtg ggccacttgc tgcagaaccc caccaaaaat ttgctggaaa ttgatatttt
    45961 ttcatcaaag cacctctttt tcttttttag ttcgttttag ttgattccat gtatgatatt
    46021 ttccatcaca atcacttttt cattttcttc ctcttctttt ttgattcgtt ttaacagctg
    46081 ttctgctgct cgttccccga ttttatctgt tggtggcgtg atcgtgattt tcggaaggat
    46141 actcagatcg tttttatcta aataatcata gtgactgaac gcaatatcct tcccgattct
    46201 gacaccttct tgattgaaac attcgatggc accaatcgac atattgtaat tgctgacaaa
    46261 aatagcagtt gccccttttt ctaataatgc ttttgccccg taatagccgc tttttctaga
    46321 atagtctcct gtaaagacct ctaattggat cgatggatca ttttggaccg cttctttggc
    46381 tcctgctaaa cgttctcttg ctacgtagtc tgtttgagtg gcagcaataa atccgatttt
    46441 tttatgtcct tgttcaccat gatgagcgat cacttttgct acactttctc gatcattcac
    46501 taatattgta tcaatacctg ctggtccttc tggacaattc aatgcgataa ctggaatact
    46561 catttttgtg agcatttcaa atccagacca ctcttcttcg gataagaaga caatcagccc
    46621 atcgatcaca tgcttcatca ggtattcaat tttttgttca attttttctg gctgatcggc
    46681 aaacccgctt aataataagc tatacccatt ttcttcagct actctttcga tccctgagac
    46741 aacttccgac ccaaaacggc tggaaatccc gtttatcaaa agtccaatag agaagcttcg
    46801 attattcttc aaccctttag caatgatgtt ttctttatag tccaacgctg aaatcgcttc
    46861 tgctattttt ttcatgttct tttccttcag ttgctgacca ttcaagtaac gggaaactgt
    46921 tcccactgaa acaccagcca tcttagccac atcacggatc gattccatac attctcactc
    46981 ctgccagtta catttgattt ctctttcttg ttgttcgatt atgatgaccg cattctttgc
    47041 atagtgttgc ttgtcatcaa tatccatata actaaagtct tcagtagagc aacttcctct
    47101 cactattaat tatacttaaa caagtatttg aaccggttca tttttaatat agcatttttt
    47161 taaaagattg caacactttt ttaaaacgct ttctgaaagt ggcggatttt ttgccttttt
    47221 aaaattttat tctaaaattt actctatttg tgggtgtttt gtgcgttttt ctcttccatc
    47281 cgtttgaagt tttacattcc aatggtataa tttctttatc ctatcaaatg ggggaagtat
    47341 tttgaagaaa ttaatcgtac tatttatttt gtctgcggga cttttgacat tgactggctg
    47401 tgatttgttt ggcggctctg accaatcctc ggacgaaaca accgattctt ctgtcttaaa
    47461 agcggaaaaa gcagatacca ccaacagtgt atatacgcta aaagtacgta aagatatgga
    47521 tttatccgcg ggacagttga catttacaga taaaaaaatg gaatggacac gcacgtatca
    47581 gacacaagga actgattctt ctgaatcatc tgaagaaaaa acagtgctga cagatatcaa
    47641 gatcactacc aaagatgacg aatatattat ttcaggaaaa gaaaataaaa aagaaacaac
    47701 agtcatattt acaaaaatcg gtaattaccg tttgaaggat gaggatggca acatctacag
    47761 tctatagtgc cgtctgttgg atcttctttt gtttctcccc ttcttttctg ctatagtaaa
    47821 gaagcaagat gattgtaaag gagacgaaat ggatgatcga tagtcctgat tattgtacat
    47881 gtaataacaa caaaaatatc catacagaat ttaatgacca aggaagcttg agcatatgcg
    47941 acaactgtca caaactagtc aaagattcat ttaaaacatt tgaatcttat cctgttcaaa
    48001 attatgtaaa aagtagaaga agataaacaa atccaagcaa aactcactat ggctgatttt
    48061 tatgaacttc aaactcagct tcttcctttg atcaagcttc atcagtaaaa caaacagtgg
    48121 atcatttcag tttacaaatc aaagagtaaa gatttattta tttcaaatat ttgaatataa
    48181 aataaccaaa cgatagaaag gcttattcta catcacgtag taatttttct ttcccgtcgt
    48241 ttggtttttt atttttagcc taattcttga ccttttgcat gctggttatc cgtaaaatat
    48301 tttccagcag gctttccttc caattcagct aatttttctt ggatctgacg gatcaccttt
    48361 tttgcatctt ctggattttc tacaaaatcg aatttatccc catccacttg tattttagga
    48421 catacatcaa aatcttcata ccattgatta tagcgggtgt tcaacgtttc ataataggta
    48481 tataacgtct catcgctttc tagctgttca tagtcacgcc cacgtttttt gatacgttct
    48541 agcatcgtgt ccaaagaaac tttgatatgg accatcaaat caggccgttt ttttggagca
    48601 acatcatcta actcgtttag catcgtatct aataagctat cgtactgctg tacttcgata
    48661 tccgttaccc gacctaaatc agcgtttaga tggaacaaca ggctatcttc atagatggat
    48721 cggtctagca cattgtcatc gttcactaaa gcatttttca ttgctaaaaa tcgcttgttc
    48781 aaaaagaaga tttgcaataa aaaagcgtac tgctcaggat ttgaataaaa gagcggcaat
    48841 acttcgttat cttcgacatt ttcataaaat ggtttagaat caatctcttt tgcaagcata
    48901 tccgtcaaac tgctcttacc tgcaccaatc gttcctgaaa tcaaaatcac tgacatacaa
    48961 cccttcctct tctataagta ctcgcctacg taagtttcaa caggcaatgt ttcaaaatca
    49021 tattcacgta cagcttggtc tgattttaaa tacgtatgga gactttgtgt aatcatcaag
    49081 ttcgtaggta ccgtctcttc ttgtacaatg atcatcggtt ttttcaacat agttgccaca
    49141 cctaattcgt aagccgttcc tggatcgatt gtttgatgct caaagtcaag gatagcaaca
    49201 acaaattctg catttgtcag ttcttccata tctttttcgt atacttcctg cgcccatcca
    49261 gcgctgaata gttcataatt gctttcttgg tgatgacgtg gagagtagaa acttgtgact
    49321 gttttgtttt cctccaacgc tttttcgata cgacttactc gatcgatttg ctcctctgaa
    49381 aaaaatggtc ctgctaaata tatttgactc attattttgc ctccttcaat aataactgat
    49441 ttttaataga tactgctcgt aattcattga ccagttccaa tggattgttg gaaaagatta
    49501 cttgatccaa ccatttttta tccataaaat cctcttttgc aaaagtttct aataattgtt
    49561 gcaagccgct gtaataaccg ccaatattca atattccgat tggcttgtcg ggcgtcagac
    49621 cgattgctat ccaagaaaga agttgtgaga attcctccaa tgtacctaga cctccaggga
    49681 aaacgaggac agcatctccc ttttcaaata ataatacctt tctttcatcg atcgtttctg
    49741 taggaataaa tgtagtaaca tcttttccag gcaattcagt ttcaaaaagc ccattcggat
    49801 agatacctgt gacttttccg ttattttcca gtactgtttt actaatcact cccataatcc
    49861 cggagtatga accaccgtaa accagttcga tttcttcttg tgccatgtat tttcctaacg
    49921 tttgcgcaat aaatttgtaa ctttctttat taccaaaacg ggaaccgcaa aaaacagtaa
    49981 gtttcataaa aaaacactcc ctaactcaaa agtctatctt cattctacac gtgttttttt
    50041 aaaataaaag actagatttt gtagtttttt agtggtatat aggatagacg ccactatata
    50101 tagtatttag aaaaaaatca tttttcagcc attttataga taaaacaaaa ataaataacg
    50161 tgttttcatc gatatgaaat gacttactta tagaagatac tgggatttcg tcctatatgc
    50221 caataacgtg tattatttta aacaacaaat acttgtttct tttattaacg aaaaaatcat
    50281 tttgttctcg tgttatgttt cactgataaa tgatgtttct ctaaataaaa aagccgacga
    50341 actaaacgat caaaatttga tcatttttgt tcatcagctt ctcctaaaaa tctttttgtt
    50401 aacgataaat agctggattt gtaaaaatat cacttgaatc gtcactgctg gaagaaaatt
    50461 cagtgataac cgcacctgtt gcgcctgcct ggaactagtg tttgatattg ggctcgaacg
    50521 tgtactgatc tctagcatga agaataattt ctttttttac tgtataataa ctttctttcc
    50581 tttttggaat agtacaggaa agtttttttg tttcctttcc ttcgacataa ggtataccag
    50641 accctttcga caacgaaagg ttctcatttt tccttcatcg tcatttcaag gagggtgccg
    50701 atgctctgga cagtttcgat cgctcctaaa ccaaaatctg tatattcgat tctttctaac
    50761 tctgattctt tcataccatt cctgtctcaa ggtacatcta ttgtactttt ttcttgtaat
    50821 ccattctgcc tctcctaact aattgtcaaa tcatcccaag atatgtcttc ttcctctttt
    50881 tcttcttgga tgatttctgc attcggatct actggttttt tcaataataa aatggttatt
    50941 cctgtgacaa aagaaccagc taacaatgca ccaatgaatg cccatggtct ggtcatcgta
    51001 gcgactgcaa acattcccga tgccggaata cggctatctg cgccccaaaa catgcttaat
    51061 ccgccaccta ctgtcccacc gactcccgtt gctatgacat tgcgtgccaa gtcgttcaat
    51121 acgataggga aacatccctc tgtgatctga cagatcccca taggaaaagc ggtcttcaat
    51181 gtatcgattt cttgacgatt gagtatattt ttacgaatga ttttccctaa gaaataagcg
    51241 atcgtcaagc cgaatggggt taccatcgaa gctaaaatca atacagtgat tggttcatag
    51301 attccttctg aaaatcgtgc cacaccccct tatccagaat aaatggtaat ccagaaacag
    51361 ccgtgtatct gcggtaataa gcctaaccaa acgaaaaata tgagaagcta tttttgtttg
    51421 gctgttctta ttacttgatg cagggcagct ttttcacaac ctcttttttt atttatgttt
    51481 cataatcaat atggtaagga tgcagctccg tatggtagac cgcctccgaa tattcaatca
    51541 tttccccaat ttgcgtagac gatttccgta tccgtttgat gccgatctgt gcgtctgttt
    51601 tcagaattcc ttgttcttct tctgttaaaa atactgccga gaaatcatca gaaaatgagt
    51661 caatagtata tccttttttc gtcagcaaac gatacaaaga ttctttgcca aattcttctc
    51721 tgctgaattg catcatttct tgtggaagat aatgaacaaa ataaatataa ggctgattgt
    51781 tcaaataata taagcgctta aaacaaacag cttgctcacc gaaaaaggaa aacaaaggat
    51841 cttccttctt tatatggtcc aattgcaaat ccagcgtttc tttccggact tcatatcggg
    51901 agttctccaa gatttgcgta aagcttgccg ctttggataa tcgattgtaa ggacgattgc
    51961 ttagaaccgt cgtcccacga ccacttttct ttgcgacata ttcatctgat gccagcagtt
    52021 cgattgcctt tcggacagtg attttgctga cttgaaacag ttcttctaat tcactttctg
    52081 tcggcaacat cgaaccgact ggatattttc ctgagaaaat atcttcttta attttatctg
    52141 cgacttccaa gtatagtaca cgtgtccttt tcataacgtc accctttgct ttactattac
    52201 taaaagaata gcacataaaa aataaaaaaa gaacacaaat cacatgtgag aagtgtgttc
    52261 tttttcattc agctgcttta attacaagtt tacgccattt gaggcaatta cttctttata
    52321 ccaatcaaaa gattttttct ttgatcgatt cagtgtccca tttccttcat cgtctagatc
    52381 cacatagata aagccgtaac gtttactcat ttctcctgtt gatgcactga caagatcgat
    52441 acatccccaa ggcgtatagc ccatcaaatc tacaccgtct gctactgcat tagccatcgc
    52501 ttcgatatga cgacgtaaat aatcgatccg ataatcatca ataatcgtgc catcctcttc
    52561 catatgatca atggctccaa gaccgttttc aacaatgaat aatggtttct ggtaacgatt
    52621 gtataattca ttcaatgcga ttctcagccc ttctggatcg atctgccagc cccattcact
    52681 tgcctcaaga aacggatttt ttactcctcc cagcaagttc ccttctgaag ttgctgcttg
    52741 tggatcagtc tcattgattg ccgtagacat ataataacta aatccgatgt agtccactgg
    52801 atattctttt agcaactgga agtctttttc cgtttgctct aatttttccg gatcgactcc
    52861 atatttttta tgggtacgcg ctgtgtatgc agggtattct ccacgtactt ggacatccgt
    52921 gcagaaaaaa ttgaattctt ggttttggat cattgttgct gcctgattga ctggattggc
    52981 atcgatgctg taagttgttg catagataat catgcagccg atttggatat ccgaacgcaa
    53041 ttcgtggccg atttttacgg ctttgctact tgcaacgaat tgattatgcc atgcttggaa
    53101 aatattttgg tactctcctg ctccattgct ttttactaat ccttgactta aagctggaaa
    53161 gtgaaaggcc gaattgattt cattgaaagt catccaatat ttgactttac tgctgtatct
    53221 ctctaatact gtctgagcaa atcgttcata gaagtcgatc agttttcgat ttttccagcc
    53281 accgtattct tttgccaaat gaagaggcat ttcgtaatga gaaatcgtga tcacaggttc
    53341 aatatcatat tttaagcact catcaatcag ctgatcataa aattctaatc ccgcttcgtt
    53401 aggagtacct tcgtctccat ttggaaaaat tcgtgtccag gcaatcgaaa aacgataaca
    53461 tttgaatccc atttccgcaa acaaggcaag atcttctttg aaacgatcgt aatgatcgat
    53521 tcctcgatga ttcgggtaaa tatatttttc ttgatcaatc gtccagtcaa attcttcact
    53581 gccgataatc gcaaagcgct gcttacctcc aggcatagca tctgcaacgg atagtccttt
    53641 tccgtctcga tcataagccc cttctaattg atttggcagc agttgctcca ccccataaaa
    53701 atttttctga aaaacgtgat tgcatcctga tttcctcctt attttacaat tgccagaaca
    53761 tcttcgtttg caataatctc tttttggtct aattcaagta tatcggcaaa tgcatcagta
    53821 ttcgtaatga caactggagt gattacgcta tagccagctg ctctaattgc ttcgatgtca
    53881 aaacgaatca ataaatctcc tgctttgacg agatcccctt gtttgactaa gatctcaaag
    53941 ccattcccat ttaattctac tgtatccatc ccgatatgaa tcaaaatctc cgctccgtca
    54001 gtcgttgtga tccctacagc atgtccagtt ggaaaaatag tcgtgatctc tccgtctgca
    54061 ggtgcaacta gttctcctac ttctggatca atagcgatac cttttcccat cacgccagaa
    54121 gaaaaaactt ggtctggtac ttcactcaat ggcagtactt tacccgttaa aggacttttt
    54181 aaggtatgcc gtgctgaagt aatcggttct cctgcattcg catgtttgtt ttctaattga
    54241 ttgtcttgtg tttgttcgtc aaatcctaaa atcaatgtac ctacgaatgc aagaacaaaa
    54301 gcaattcctg tagctattac tgctactgtc acatttgact ctacaccatc caccgtatta
    54361 atgaaagtcg gaatcgtcaa taagctgacc aaactgctgg agaaagcttt tactccggaa
    54421 aatccgataa tagctccacc aatacctcca gaaatacaag cggcaatgaa tggtttcttc
    54481 aaaggtaatg tcacaccata cacagttggt tcagtgattc caaataacga agtcactgta
    54541 gaagaaatcc ctaatgctcg aagtttcgta tcttttgttc gcaatgctac agctaaagca
    54601 gccccacctt gtgccaagat ggctggaaga agcattggca tcaatacatc aaacccatat
    54661 tgttcgatat tcaaaaacat gatcggtacg aatccccgat gcatgccgaa catcacgaat
    54721 acttgccata aacttcccat gattagacct gctacgattg gactaaatcc ataaatcgag
    54781 ttgaagagac cgcctaaagc attaccggca accgtcccga ttggtcctaa agcaagaaat
    54841 gttaatggaa ccatcactac taaaacgaca agcggaacta aaatcatttg cagaaattgc
    54901 ggaatcactt tttttacgaa tggctctaat ttactttgaa cccatacggc tagaatgatt
    54961 ggtatgacag aagaagtata accagatggt gaaagaatca ctgggatccc aaagaaactg
    55021 atagcttctc ccgcaccagc aactgctgtg atagctggat acacaagtgc catagcaatt
    55081 gctactgcta aaaactggtt ggctttgaac tttttagctg ctgtaaaagc aagcatgaca
    55141 ggtaagaagg taaagacacc atctgcttca gcaaacaata tttgataagt accgctatca
    55201 gctgtcagcc agcccatcac cgttgccaat gataagaatc ctttcaatac cccggcggct
    55261 gccatcgccc ctagaaaagg tgtaaatata ctagagataa tatcgatcag acgattcaaa
    55321 atatttttgg actcttcttt tccatcctcc gaaactgcaa gaccttcctc atccacgatc
    55381 gattgataaa catctgctac attactgcca atcactactt gatactgacc gccactttgt
    55441 acgacttgga tcacgtccgg gtcttctttt aacttttgcg tatctgctaa agattcatct
    55501 tttaatctga atcgcaaccg cgtagcacaa tgtactaaac ttttgacatt cttttgtcct
    55561 cctaccgctt cccatacacg acgaccaact gcttgattat ccattgtttt ttccttcttt
    55621 cttttttatt taaaaaataa aaacccagat tgaaaaaaac agtcacaaac attcgtgaac
    55681 ctattttttc aatccaggtt ttgcctgcca tacagtaaca atccttatga ttggatgctt
    55741 tttttctcac ttactactct ttgaatatga atcgttaaat aaatctgctc tgcgatcccc
    55801 atttcctgtt tttgcgtttg tcgaagatac tcattgatcc gttcgacaca atgaaacgct
    55861 tttggatact ttccttgaac taatgcgtag agaaattcat cttgctcgtc agatccttcc
    55921 cctttgagat aacgctgggc aaaaaactgc agatgagtaa tgatacgctg ataattcaaa
    55981 gattcttcat cgaacacgat gccgaagaat ttactgatga tactcagtat atctcttacc
    56041 attgtggtag cggtcatcgt cacttgcatg tcttcgtttc cttgttggct attcacaaaa
    56101 tgaaaggcaa taaaaccggc ttcatcctca gaaagttcca cacccgtctt ttgtttcacg
    56161 aacaaaagag cctctttcgc tgcttgatat tctttcggat aaaatttttt tgtttcaaat
    56221 aataatggat taggcagttg gatattttct tttgctcgtg tgatcgcata atgaagatga
    56281 tcggtcaatg tcagataaat attaggcgaa aattcaactt ttagtttatc ttcagccagt
    56341 tcgactactt gtttgaccca ttcgatctcc tctgccggaa tatcagccat cagctgctgg
    56401 aattggtttt tagcaaaaga atctcttaaa acaaattctt tttggaccgc ttctggcgaa
    56461 atctcgtccc caatcttttt ttgaaaagct agtccttttc ccatacaaac gacttcttcc
    56521 ccgaatccat ttttcgaaat gacgacattg ttattcaaaa ttttatcaat tttcattttg
    56581 cttcgccctc tcaagaaaaa taaaaaaacc tagaattcgc ctactgtttc ttagctgaat
    56641 tctggttttg cctgccgtac agtaacaatc catttcttat tcaaaatcat cataccatcg
    56701 aatttttatt tatgcaagcc ttttcatata aaaaataaaa aaactggaac acaagtctat
    56761 ctagaaaaca accaaatcag agatgcacct ctttgcgttt ctcttttttt cagcaaattg
    56821 gtttttcctt atcgattcat atcctttggc tgtttttaat ctgtaacgac ttatgtccca
    56881 gtatcctaaa atgtttttgt ctctagattg aaagcagatc tcatatcatc tgtcgcatct
    56941 tttatcgatg taacactttc cagttctcct tgtacaacta gtcgcgtgac ggcgtccatg
    57001 tcgccacaag taattagtgt gacaatttct tttccctcga cttcattcag taattgggta
    57061 tctgtaggag caactttttc tttaaaaaat gttttgtatg tatacacatt ttctaaatct
    57121 gtcaaataga tcttggcacc catctctaaa ttttctagcg gtgtaaacag caattcaggg
    57181 ttcgttgcac gatggcttgc taaggcataa tttccttttc ccatttcttg atctggagat
    57241 aatgttcctg ctccgtaaag tagtgacgta ttatccaagc ctttgaaaac cggtaaattg
    57301 attgaaactg aagggactgc gatactagca atcactggta gatctgttcc tctcatttgc
    57361 gacctcatga ccccttctgt cgtcattggt tctaccgcat caaagtcaaa actgacatct
    57421 ttatcgttat tctttttcag atcttctttt gttacattcg ctatagcata tttatctcct
    57481 gtctctctga caaaataatc cttgatctgt tcattgaaaa tcaaagctag tcctactaat
    57541 aacaagagga acaagaggat attgactagc catctttttg cttttccttt ttttttcatt
    57601 ttctctccct ccagactcat tttactgttt ttcttttgct ctttccaaca gccgaatgac
    57661 acatttctac aactctgtgt atttttttcg tacacctgcg cttttttcta caggatattt
    57721 caaattattt ttgatcaatt tttcttcaat gatctcatct agatccaggt gaaggttgtc
    57781 tgccatcatg tatgaataga tcaatacatc tgccagttct tctttgattc gttctaattg
    57841 tgtttctttt acttcttccg gcgttttcca ttgaaaaagt tctaacaatt cagatgcttc
    57901 caaagaaata gagatagcca aatctttttc gttatgaaat tgtcgccaat tacgttcatc
    57961 tctaaactgg ttgacttttt ccatactatt catttgcttg ccttcttcca atcacttctt
    58021 acttgattaa tcataccatt tttttacata aaaaatagat gatgctttta aaaaaactcc
    58081 tgttttcttt tatttaaaat aaatgtcata gaaaatcaat aatttaggta gccaccatgt
    58141 gtatgcagta gcgtgtcttc ttcccttgag gctttttgtt tttcgatacg ttcttcttcc
    58201 caaacagaca acgctactag aatctctctt aatgattttc cttcttcggt gagcgagtac
    58261 tctactttag gcgggacttg gttataggct tttcgatgga tactttcatc ttgttctaac
    58321 tctcttaact gctgggtcag catttttttt gtgatcgtag ggatttgctt gcttctccac
    58381 gcctaaaata agcgatattg ctcttccctt tatctggtgt aagcaaagca gttagcattt
    58441 tgacgatcgt gggttccgct tttcacttct tatctactta gtatcatgaa gtatactttc
    58501 gtactaatac taagtagcgg aagtgtcaac tactttgttt aactacatag caaaagaaac
    58561 agaagggtga caacggtcat cacgcttctg tttcttttga atatgctgtg ttgttgtctc
    58621 tatctccgtg tagactttcc tcaaacttat cctttgacag taagtcagta ggctgatttg
    58681 ctctttctag atgccagata catttctaca aaaaaatagt gattctgttt tgaaaattcg
    58741 atttttagtt ctgcattttc gtaaacatcc aacatttttt tcgcactgta tagacctaac
    58801 ccgccatttt ttttagaggt ttttcctctt ttgaccatct ccgatagcgt caaattcgtt
    58861 ggatttgcct tgttcgctat actaaaacgt gatccctctt tttcttccag cagattgatc
    58921 gtaatcggac tctgatcggc tacacgatga tcgaatgcgt tagaaataaa aatagataaa
    58981 cagcgaataa aaacaaccag atcaatcggg attgttttca cttcgacagg aataactact
    59041 tggatcggga ttttctccga ttctgctttt tctagaaatt ttaaaaagac acccctgaca
    59101 gctggggata caatatttgt tagctgttgt atttgcggtt tttcaaaaat agctgcagaa
    59161 tgatcttcta atatttctaa aaattccaac gtttcttttt tattttcctg aagagtaatt
    59221 tttaatgaaa acagtagact tttataatca tgtttgaatt cctctagatg atggtaatat
    59281 tcttcattta tttgatacag ttgaagcgat tcctgatgtt gtctttcttt tacttggaaa
    59341 ctgatcaaca taatggaaaa aaacaaagaa aaaaaactga tgactatcga caaaagagat
    59401 agaagcagat aattagatga gcggaagtga acacttccca ataccaagta aatcacgccc
    59461 cagcttaata aaatacaaat acctccaaaa tagtgttcag ttggataagt aaaaagcaag
    59521 ttgagaaatc tcgtctgttt gtctatactt tttactaata gatagaaaat aaatgcgata
    59581 agtagtgcaa aaaaacttgc tgttccaaga tgtctctttc ctaaataatc catcagatca
    59641 aaaaccagaa aaaagctgag tccagtgata gcaaaaaaaa gattactgat caaacagctg
    59701 ataaacaggt ttttcgtagt aaaataaaaa agaaacatca ataataagcc aactaggtaa
    59761 ggaaagctca ctttagggat aggaagtaaa agtgtcacca ccatcaaagc tatacaacca
    59821 aagctgtttt ttactagcag ttgtttcgaa aagaataaat agatacctaa atctaaaatc
    59881 aaagtaaagg tcaaataaag tgtcactgtt gcgaccatct tattcctcca ttgttttgtt
    59941 tcaaaatgta cacatatttt tttaatagga gcctgcatat gaaaaagtat tttatcatag
    60001 aagataacca gtttcacagc gaatggatca tcagagaatt ggtcaatcat catatcgaga
    60061 atatcgtgca gctcacttct atctctcaaa tcaatcagtt gatccagttg ggcactattg
    60121 ataatcacga tgttttcttt atcgatatcg acttgaagca atcactcaca ggtattgatg
    60181 tcgccgaaca aatacgtaaa aaaaatgcac atagtaatat tgtttttttc accgcttata
    60241 cgaattttgc agtagagatc atcaatcgga aaatccttcc gattggatat atcagaaaag
    60301 acaggaattt atctgaacaa attcgtcaag tgctagaaca gatcaattgg aaagaacagc
    60361 agatcgtaaa tgtcgataaa atctttattg atcaaaaagg tggaagtctc gtcctgtatc
    60421 ctcgggaaat tttatatatt gaaagtatca aaggtttgaa aaaccaagtt atggtcaaaa
    60481 caactacaga agataaatta gtgaatactt ctttaaaaca attgaagaca gagttagaaa
    60541 agtatgctta ctttttactt ttaaaatctt actgtatcaa cttatcgcaa ttgcagaaaa
    60601 ttgattctgc tcattatgtg ttagaatttt tcaacggtga ctccttactc gttggtcgaa
    60661 agatctttga aaagacaaag gaacgtttcc atgacttcca aaaaactgct tcgagttgat
    60721 gaataagagc tgagacgtaa gcatagcaga tttcagggag tgggccttga atcggctcca
    60781 ctccctgaaa tcgttgatat gattttacgc ttcctcttct gtaggagcat ctgttaacga
    60841 ccatgtgaat gttgaaacgt agttgtctac agcataattg gatacaggaa caaataattc
    60901 cactgattgt ctcccttgtt ccacattctc tcctaatgaa taaatccatg tgccaatccc
    60961 ttcatttgct cctgcagtta ctagatctac tgcctgatct gctgggattt cgattccttc
    61021 agcgccagtt ccagaaacgg tagatggcat tccactgcta ttagttgcgc tatttcgaat
    61081 cacggcgttt ttgaatttca cctttgaacc agttagttta ttcgtatcag atgttgtacc
    61141 aataaattct gttcgtttga ctgataattt ccaaccggca taatttccgc ttttatccgt
    61201 tacttgtacg aaattctgta cttgtgtttg tgctccagta ctgtccgttc cgctttgtaa
    61261 tgcagcatat actgtttttg ctgtacctaa cttgattgtt ccaaaatcaa gatctggagc
    61321 ttggtcaatc gtcagtaatc cggatgttcc ctgatttggt tcgtcaggtg taaaaggagt
    61381 atcaggatca tttggatcaa caggaggaac tggaacaacg tcttcaggat ttgcagcatc
    61441 tggtttggtc acagaaatcg ttccttctgt agtggctgag ctcttattct cagcaaaaac
    61501 actttgtgta cataataata gacttccagc tagaacggta cttaatgcaa ttgttttttt
    61561 catttctttt tccttctttc ttttaaaata aataatccag caaataagcc aataagaatg
    61621 actccaacaa gtatcagcca tcgccagtca aatgtttctg gctgattttt taaatcaata
    61681 tccttttcat tcaattggtc cgctgcatca tcagttattt caaaatcggt atggagattc
    61741 cagcgttttt ctttccaagc acccttcaca accgcttgat aatgtcccgg agctaatttt
    61801 tttccttcta atgggacaag taaatcaaaa tttgtattgg gtgctacatc tattttttct
    61861 ttttttattg aaaggtaagg ctcttttgtt tcttttttgt agatttcaac cgatgtagtc
    61921 aagccgaaca tataagaagc atttggattt tgaagattcg ccttaatcac atttcttcca
    61981 tcgctttgat ccgcataagc tttttttaat ttcaaatctg gggaagactc tttttcgcta
    62041 tttcaaatca agattgcttt cacttgagaa aactgattga cgacaccaac tgtacttttg
    62101 ttagaaacgt tctctttttt ttcttgaaac gtgatccctc ctgcgaccac accagccagc
    62161 tctttttctg gcgctttcag tgaaagtgtc tcagtgaccg cactttgagg aggaacagtt
    62221 atttctccca ctgtttttgc taagtttttc atatcataac ttaatgtttc atcaaattta
    62281 ctttttgatt ctccatattc gatcacaccg tttgaattcg tcgtagcgga gtggatcaat
    62341 gtctcaactg ttactgattt atctgttcgg ttaaagaccg tgtaggggat ttcctgtgtt
    62401 ttcccaggct ccatccttac atttatatat gttttgttct tatcaatctg attagccggt
    62461 aattcaaaat ctaccgcaaa attgaattcg gaggcatgga caggtgtctg ccaaaggatg
    62521 aatccaagga gaaggatcat aagtaaagag cagttgtttc gatgactttt attccatatt
    62581 cgattcatca atcatcctcc ttgatcttta ttctgtcggc gcatctgcta atgtccatgt
    62641 tatctctgct gagtaattac cagctatctg attttctttg gttaagacaa gtttaaacgc
    62701 ccgaccgtta tttaaatatt ctgaataatc tgcttgcaca ttcgttgaat tttgtcctct
    62761 gacaatttcc agttcttctt cattcactaa ttgatactcc tctcctttct tgaaatatag
    62821 gacatcctgc atagaaaatc ctgcatcatt tgtgaaaggc tgagataatt ttatcgttaa
    62881 gatccacgga tttttcgttt ctcgctgatc ataaatccat aatttttcct gtatatccat
    62941 ttggccaaat gcttcttgtg tcaaaccagt tgctttgatc tgaccaaaat cgagtttgga
    63001 aggggcaaga aatgagagac caccttctac tacgatcttc gtagcaggtg caaaaggcgg
    63061 ggcatcatga taaacaactg gcgtatcact tggattgtga ttcccaattt catcgttggt
    63121 acttggcttg ttctgcacac ccttagcagt agcttctcct gcttggtcgt ttaatacaat
    63181 ttctaataca tctcctgcaa aaagttgatt ttctgggata gaaagaacaa attcccctgt
    63241 atcatcaata gttgtttctg acaaaacagc ttgatttttt ttcaatgaaa ccaaggctcc
    63301 tgcttcacct gtacctgtga tcgtgcgaga agctgcattg atcaatgtgg tacttaaatt
    63361 agcaggttca ggaggaatca catcgactgt cgttttggca gagtccagtt ctgtcacttc
    63421 ttggccacct cttacttttt tagcagcaac aacttgcact tgatcctccg tttttaatct
    63481 ctcatcattg ggtacttgaa cttcgaacaa gccgccttgt tcgccttctc cccatgggtt
    63541 atatcctgcc ccttcatttt catctgtttg tccttgcgtg agagcagtaa tttctacaga
    63601 tgatttcgtc gtatctgcgt aagtaatacg taaagtcact tctacttcgc ctgtccaagc
    63661 atctcttggc tttgcctcga caccttcggg aacaacgaca tgcccataga tttttttatc
    63721 tgcattcgtt ggcactctta aggattcaat catcgggagc tgattattgg cactgattcg
    63781 attataattt ttcattccgg cacctgtctg agcaaattca tttgttaaac ctgcactagg
    63841 gcttgatacg aaattgccta agtcggtccc attgatcgat aagtctgtca tatcagaaaa
    63901 ataatccggg tctttttcaa agttatcgaa tgttgcgcct gtttttcgcc ataaagcgat
    63961 ttctgaatgg gcgatcttta aagaagaatc tccatttcct tggaataccc agccgcctat
    64021 gccattggtc tcgcttccaa tccgtttgtt ttggaaatca aaataggtag gagattctaa
    64081 gtccacattc aacacatccc cactgatgat gccggtctta tcttcccctg ttttcccgac
    64141 catgactaaa taagtttttt cacctgccgt aaaatttaaa tcaatatctc cagttgtatc
    64201 tacagttgct ccggaatctg cgcgtatatc gatatttgat ttttctccag tgactgtaaa
    64261 atcagctgaa cctggacttt ctagaaaacc agaagcgtca taaaattgga tcgcttgatt
    64321 tccttgattg agtctgttca cgtaagattt gccatcacct gtatttctta ccctcaatga
    64381 gcctccattg gttactgtca gcttttgcgt accattctca aaacgaactg cgctggaacg
    64441 cccactctgc ttatcgatgt cgataactga tccggctcct tctacgatca aagaaaggcc
    64501 ttttcctaca aaacgcatcc cattgttccc gggttgatcg tcatctccag ccattttgac
    64561 cgagatttgt ccaccacttg atacatggat ttgactagaa tctccttgca agcgaaatcc
    64621 gcccgtagtt gctgtattga tggtgatttt cccaccattc gatacagtga ctttcgatcc
    64681 tgaacctctc atttggatga ttccgcgatt gttgtcattg gtaatattga tctttgtatt
    64741 taccgaaaat tcgcttcccg aaccactgac ttcaaacatg acattaccag gtagcgtatc
    64801 gactgcctct ccttcatcgc taacgccatc tgcccgatca taatcaagag agacctttga
    64861 accgcttaag atatggattc ctggcgctgt ttttccacta ccagtctcat ctcctgaatc
    64921 tagaaaaccg tagaatttcc ctagatacgg tccataaagt tgactaccat tgatcacatt
    64981 caattgatag cttctacaaa aataggcttt acccggagcg gcattcagtc cttccttcac
    65041 tggttggaca tccaacacac cggaaacacc atctaatgtg acagttgcac gaggagcgta
    65101 aatcagtcct aatttggacc catcatacaa atcaagtgtc ccggtaaatt ttatttctcc
    65161 cgtaaaatga tatctgcttc cccaaaaatc ggtcatataa gcaatgataa tggcatttcc
    65221 atcgatattt ggtgtcaatc cacctggatg tgcagctgtt cctttaaaat tctggaactc
    65281 gatcgactga ggtatctcat tgatataaat atggtgtgtg ccgaaattgt gagcgaatcc
    65341 accaaaatca ataacgatat ctcgtttgat ctctaattcc ctgtctaaag tcaaaggctg
    65401 aatcagtcgg atcttagtta ctgattcgtc tgcgagaagc tgcgccagtt cttctgttgt
    65461 ggcagcttcg ctgtacccat ccgctgaaac agctcttgcc agtgccgctt cactgctatc
    65521 agacgtttgt tcagctactt ctgaggtctc acttgtctta ttttcttctt gccttttatt
    65581 agtagaagcc tcactttgag attctgtagt ttcagaagta atcgattctt ctgaaaaagt
    65641 agtcttgtca aaactttgat tctctgattg cgttgattcg tttgttttct ctacatcggc
    65701 aaaaacggat gtcaccatca agagagggaa gacaaacaaa atcgacgcaa tcagcttttt
    65761 tacacattga ttcattctcc accatcctct ctgacgtttt gacaatttta ttttatcgtt
    65821 ttaatctatt tccaattgta ctttcatatt taaaatatgt atttttatgg aaaaattttt
    65881 ctattaggtg taaatttctt ttttgtacaa aaaaaaggct gtgacaaaag tcttgtcaca
    65941 gtcccttatc ccgaacaagc ggtgttctcc cgccaactcc tcggtcacaa cctccatttt
    66001 aacaataaac ctgtaaatgt gagggcaata ctttgattgt cacaggtaat ttatcccctt
    66061 catctccatc aacattcgtc gtcaattctt cctcatcagc taatgacaca actaattgct
    66121 cacaagtaag gtaggctaga tttttcgtag attcatctac tccttttaat aggtctggca
    66181 atgttttaag cgtatcgatc aaagaagaat cttttaaata aaccaaatgg agcttaccat
    66241 catccacttt tgcatttgga agtaatgttt caaaaccgcc gactgagtta gttagtccta
    66301 tcagcaaggt actgctctca atttcttcct tctttccatc aacttctacg tgaaacgagt
    66361 aggattgtgt ggaagctaac tgtttgattc ctgacatgaa ataagctagt tttccaaatt
    66421 tcgtcttctt ctccgcatct acatcattga ttgattcagg gatagagcca attgcgacga
    66481 cattcataaa atagtcgtca tttatttttc caatatccaa tggcttgact gattcaatcg
    66541 aaaaatgatt gatcgcttcc tcaggttcta gcggtatccc taatgctcga gccaagtcat
    66601 tcaccgtacc caatgggaaa aatccgaaat ttggccgatg ttcttgttca gcgattccac
    66661 tgattccttc gttgactgtt ccatctccgc ccatgacaaa cacactatga tacccttccg
    66721 tagccgcttc gcgagtaaag ttttttgcat cccctgcttt ttttgtatgc aagacgacca
    66781 cttcatcaaa caccgattcc aattttgcta tcgctaattg ctcaaattcc tttgcttgtt
    66841 caccgcctga actggggttt accactaata aaactttttt catcactttc tttcccttct
    66901 gattgatttc tttaatataa cgatagcata aagaaaaaaa ttaggctgtg atgaagtact
    66961 gttcatgaaa cattcattta ttttttgaca agataaaaca tccatactat gaaactgagg
    67021 aaaattaaaa caatgatata ggaaataaaa gaaaatgata ggcattgtat acctaataaa
    67081 gtaaaaatct taggtggcag ataaaaacac aaaaatagca agagaatgta atagcgtttc
    67141 ttccactttt tgttatcaga ccttatagcg atcagaggaa taccaaatgc ataactgaaa
    67201 aactcttgat gtactgttaa tatgtcacgg ccttgaataa taaatgcgac aatccccgct
    67261 ataattagtg tgcctaacac caaaacggaa aattgatgaa tatattttat atacaaatga
    67321 tccattgtac ttcctgcctt tttatttgtg agaaaaacaa atccgtatac gtgaataact
    67381 tctagaaata aaagaaattt gcaaaaccac tccatttatc tacttctacg tgcatttttt
    67441 gatattgttt tctttattta agattataca gaaaaatatc tagggtcgct ggattctaag
    67501 taaaaaaaat ttttaaaata aaaagaacta aatcaagtag taattaaact actgttgatt
    67561 tagttctaat aaaaagattt agatgaacag ttttattgat ttaaatagtt catatttttt
    67621 atttataccg cttatttact acttgctgta ctagaatctg aagtgaatgt actcaaaaca
    67681 ttttcaaagt ctttgtcttt gatttttaca ttagcgtctt tcaatacttc gccaattact
    67741 ttattctgga aggtactgtc acttaatttt gtgtctgttg caatttcttt caattctttt
    67801 ttgtatttat ccatgtcgtt gcctttgttt tggtttttca ccattttaac cacataatat
    67861 tccgtagtat aagttgaagc attggtagaa gtgatcacat cagaaacttg accatctttc
    67921 aacttaaagg ctgcttcttt gacttctgcc ggaaccgttg tagtagtgga atcaaatttc
    67981 acttttccgc catcttcttt tgtcgtagta tcagttgact tgtctttggc taatttagag
    68041 aaatcgtctc ccttatcagc agctttcttc acatcttttg catcatcttc acttgctact
    68101 tggataattt gagcttccac ttctggatgg aaagattccc aagccgtttt taaatcttca
    68161 tcggtcaatt tgatatgttt ctttagacct tcttgaaagg ctagactttg tttgatactg
    68221 tctttgaatg tttgttctgt gtaacctgca gatttcaatt gtgaatcaaa tgaatcaccc
    68281 aattgtttct ttgtttgatc atattgagaa tcgatttctt tggttgatac gtcatctcca
    68341 tatttatctt caaatacttg ataaacgatc aactgttgca cagcggaatc tgctccttgc
    68401 atttttttca agctatccat tacttcttgt tcgctgattg taccgccttt ataactaacg
    68461 gctgtttttt caccggcatt cgtacagcct gacaataagc cagtgcctag tacgatggca
    68521 agtgctaaaa atcttttttt catttatcaa ttctcctact tttcgttttt tgcgaattgc
    68581 taaaaacaaa ggtgttcaag atgaaaatca tcaagaacac ctttactaat ctgctcgata
    68641 cgtcctgaat agatataagg ctacacaatc cagaaagttc gctcagcaga ataaatgtca
    68701 agtagcacat cacaccttaa tatactactt cttttgttac cgtttttcaa gtctatcttg
    68761 cgttccgtat ttaacgtaac taattcttag aacaaatgtt atcgattttg tttttcttct
    68821 tccgctgctg ggatattcaa atcagacact tttatgttcg attttccaga catttttttg
    68881 ttgtttttat tatgatatat ttttatacaa
//
LOCUS       DACEQH010000007        67832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.007, whole genome shotgun sequence.
ACCESSION   DACEQH010000007 DACEQH010000000
VERSION     DACEQH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.007"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            121..1335
                     /locus_tag="IW833_000445"
     CDS             121..1335
                     /locus_tag="IW833_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1306588.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(2045..2233)
                     /locus_tag="IW833_000446"
     CDS             complement(2045..2233)
                     /locus_tag="IW833_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306589.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            2324..2533
                     /locus_tag="IW833_000447"
     CDS             2324..2533
                     /locus_tag="IW833_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ1306590.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            2645..4258
                     /locus_tag="IW833_000448"
     CDS             2645..4258
                     /locus_tag="IW833_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ1306591.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            4293..5504
                     /locus_tag="IW833_000449"
                     /pseudo
     CDS             4293..5504
                     /locus_tag="IW833_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            5527..6453
                     /gene="glsA"
                     /locus_tag="IW833_000450"
     CDS             5527..6453
                     /gene="glsA"
                     /locus_tag="IW833_000450"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ1306592.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6661..8121
                     /locus_tag="IW833_000451"
     CDS             6661..8121
                     /locus_tag="IW833_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306593.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            8342..10105
                     /locus_tag="IW833_000452"
     CDS             8342..10105
                     /locus_tag="IW833_000452"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ1306594.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            10189..10647
                     /locus_tag="IW833_000453"
     CDS             10189..10647
                     /locus_tag="IW833_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ1306595.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10756..11439
                     /locus_tag="IW833_000454"
     CDS             10756..11439
                     /locus_tag="IW833_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1306596.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            11586..12200
                     /locus_tag="IW833_000455"
     CDS             11586..12200
                     /locus_tag="IW833_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ1306597.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            12216..12422
                     /locus_tag="IW833_000456"
     CDS             12216..12422
                     /locus_tag="IW833_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306598.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            12963..14093
                     /locus_tag="IW833_000457"
     CDS             12963..14093
                     /locus_tag="IW833_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1306599.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            14185..14940
                     /locus_tag="IW833_000458"
     CDS             14185..14940
                     /locus_tag="IW833_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1306600.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            14998..17037
                     /locus_tag="IW833_000459"
     CDS             14998..17037
                     /locus_tag="IW833_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ1306601.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(17146..18297)
                     /locus_tag="IW833_000460"
     CDS             complement(17146..18297)
                     /locus_tag="IW833_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1306602.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(18451..18981)
                     /locus_tag="IW833_000461"
     CDS             complement(18451..18981)
                     /locus_tag="IW833_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306603.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(19080..19430)
                     /locus_tag="IW833_000462"
     CDS             complement(19080..19430)
                     /locus_tag="IW833_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ1306604.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(19587..21116)
                     /gene="ahpF"
                     /locus_tag="IW833_000463"
     CDS             complement(19587..21116)
                     /gene="ahpF"
                     /locus_tag="IW833_000463"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ1306605.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMVMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(21126..21689)
                     /gene="ahpC"
                     /locus_tag="IW833_000464"
     CDS             complement(21126..21689)
                     /gene="ahpC"
                     /locus_tag="IW833_000464"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ1306606.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(21833..22495)
                     /locus_tag="IW833_000465"
     CDS             complement(21833..22495)
                     /locus_tag="IW833_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1306607.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            22679..24325
                     /locus_tag="IW833_000466"
     CDS             22679..24325
                     /locus_tag="IW833_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1306608.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            24316..25398
                     /gene="uxuA"
                     /locus_tag="IW833_000467"
     CDS             24316..25398
                     /gene="uxuA"
                     /locus_tag="IW833_000467"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1306609.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(25506..27059)
                     /locus_tag="IW833_000468"
                     /pseudo
     CDS             complement(25506..27059)
                     /locus_tag="IW833_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            27207..28348
                     /locus_tag="IW833_000469"
                     /pseudo
     CDS             27207..28348
                     /locus_tag="IW833_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(28474..29553)
                     /locus_tag="IW833_000470"
     CDS             complement(28474..29553)
                     /locus_tag="IW833_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1306610.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(29656..30096)
                     /locus_tag="IW833_000471"
     CDS             complement(29656..30096)
                     /locus_tag="IW833_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1306611.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(30153..30563)
                     /locus_tag="IW833_000472"
     CDS             complement(30153..30563)
                     /locus_tag="IW833_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1306612.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(30743..31177)
                     /locus_tag="IW833_000473"
     CDS             complement(30743..31177)
                     /locus_tag="IW833_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1306613.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(31277..32125)
                     /locus_tag="IW833_000474"
     CDS             complement(31277..32125)
                     /locus_tag="IW833_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1306614.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            32514..34280
                     /locus_tag="IW833_000475"
     CDS             32514..34280
                     /locus_tag="IW833_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ1306615.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            34382..35641
                     /locus_tag="IW833_000476"
     CDS             34382..35641
                     /locus_tag="IW833_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1306616.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            35788..37089
                     /locus_tag="IW833_000477"
     CDS             35788..37089
                     /locus_tag="IW833_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1306617.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            37092..37943
                     /locus_tag="IW833_000478"
     CDS             37092..37943
                     /locus_tag="IW833_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1306618.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            37956..38771
                     /locus_tag="IW833_000479"
     CDS             37956..38771
                     /locus_tag="IW833_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ1306619.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            38922..40088
                     /locus_tag="IW833_000480"
     CDS             38922..40088
                     /locus_tag="IW833_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1306620.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPTQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            40318..40965
                     /locus_tag="IW833_000481"
     CDS             40318..40965
                     /locus_tag="IW833_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ1306621.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            41049..41993
                     /locus_tag="IW833_000482"
     CDS             41049..41993
                     /locus_tag="IW833_000482"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ1306622.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            42128..43579
                     /locus_tag="IW833_000483"
     CDS             42128..43579
                     /locus_tag="IW833_000483"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ1306623.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            43600..44436
                     /locus_tag="IW833_000484"
     CDS             43600..44436
                     /locus_tag="IW833_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1306624.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            44436..45095
                     /locus_tag="IW833_000485"
     CDS             44436..45095
                     /locus_tag="IW833_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1306625.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            45221..45910
                     /locus_tag="IW833_000486"
     CDS             45221..45910
                     /locus_tag="IW833_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306626.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            45916..46872
                     /locus_tag="IW833_000487"
     CDS             45916..46872
                     /locus_tag="IW833_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ1306627.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            46916..47428
                     /locus_tag="IW833_000488"
     CDS             46916..47428
                     /locus_tag="IW833_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1306628.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(47684..48232)
                     /locus_tag="IW833_000489"
     CDS             complement(47684..48232)
                     /locus_tag="IW833_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1306629.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(48321..49502)
                     /locus_tag="IW833_000490"
     CDS             complement(48321..49502)
                     /locus_tag="IW833_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ1306630.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(49738..51246)
                     /locus_tag="IW833_000491"
     CDS             complement(49738..51246)
                     /locus_tag="IW833_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ1306631.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            51405..51596
                     /locus_tag="IW833_000492"
     CDS             51405..51596
                     /locus_tag="IW833_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306632.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            51799..52716
                     /locus_tag="IW833_000493"
     CDS             51799..52716
                     /locus_tag="IW833_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ1306633.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            52709..53782
                     /locus_tag="IW833_000494"
     CDS             52709..53782
                     /locus_tag="IW833_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306634.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            53930..54448
                     /locus_tag="IW833_000495"
     CDS             53930..54448
                     /locus_tag="IW833_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306635.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            54429..54821
                     /locus_tag="IW833_000496"
     CDS             54429..54821
                     /locus_tag="IW833_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ1306636.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            54897..55832
                     /locus_tag="IW833_000497"
     CDS             54897..55832
                     /locus_tag="IW833_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ1306637.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            55965..56744
                     /locus_tag="IW833_000498"
     CDS             55965..56744
                     /locus_tag="IW833_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ1306638.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            56737..57054
                     /locus_tag="IW833_000499"
     CDS             56737..57054
                     /locus_tag="IW833_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1306639.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            57298..58908
                     /locus_tag="IW833_000500"
     CDS             57298..58908
                     /locus_tag="IW833_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1306640.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            59065..59913
                     /locus_tag="IW833_000501"
     CDS             59065..59913
                     /locus_tag="IW833_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1306641.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            60144..60623
                     /locus_tag="IW833_000502"
     CDS             60144..60623
                     /locus_tag="IW833_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306642.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            60656..62884
                     /locus_tag="IW833_000503"
     CDS             60656..62884
                     /locus_tag="IW833_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1306643.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            63013..64656
                     /locus_tag="IW833_000504"
     CDS             63013..64656
                     /locus_tag="IW833_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1306644.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(64729..65274)
                     /locus_tag="IW833_000505"
     CDS             complement(64729..65274)
                     /locus_tag="IW833_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ1306645.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(65297..65782)
                     /locus_tag="IW833_000506"
     CDS             complement(65297..65782)
                     /locus_tag="IW833_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1306646.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(65772..66584)
                     /locus_tag="IW833_000507"
     CDS             complement(65772..66584)
                     /locus_tag="IW833_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ1306647.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(66723..>67832)
                     /locus_tag="IW833_000508"
     CDS             complement(66723..>67832)
                     /locus_tag="IW833_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1306648.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        22665 a        12042 c        13339 g        19786 t
ORIGIN      
        1 tttatatcca tacacacaaa gcagttagtc ttaggattga ctgctttttc tatacagatt
       61 tttaatgaca ggcaattatc aattatggta gttgcctttt ttgattggag gaattgaaga
      121 atgaatgata aaaactttat tgaagaacta agacaaaagc gtgaggaata cggagtaaca
      181 caaacaaggc ttgctgttgc ctgtggtatc agccgtgaat attacaaccg cattgaaaaa
      241 ggaaaacagc cattgaatga cgaattaaga gaaatcatag aaaaacagat tgagcgtttc
      301 aatccgcaag aaccactatt cttattgatt gattactttc gtgtccgctt tcctactacg
      361 gacgcattgg cgattatccg tgatgtatta caactgaaat ctaattacat gctttatgaa
      421 gattatggaa aatacggata tgaaagcaaa tatgtacttg gcgacatcaa tatcatgtgt
      481 tccatgcagg agcatttagg tgttttattg gaactgaaag gcaaaggctg tcggcaaatg
      541 gaatgttatc tgctggcaca ggaacgctca tggtatgact tcatgctgga ttgtatgacg
      601 gctggtggtg tgatgaaacg gcttgatttg gctatcaatg ataaagcagg aatattggat
      661 attccaaagt taaaggaaaa atacaaggct ggcgaatgta tgtcctactt ccgtatgcag
      721 aaagattaca gcggtacaga aaaatgcggt agcgatttac caaagaatac aggagaaacc
      781 ttatatctcg gttcaacaag tagcgaatta tatatgtgtg cttatcagaa aaattatgag
      841 cagtatgtca agaatggcac agaaattgaa gatacggaga ttaagaaccg ttttgaaata
      901 cgcatgaaga acgaacgagc ctattatgcg gttgtagatt tactgaccta tcgggacgct
      961 gaacgcaccg ctttttctat catcaatcat tatgtccgct ttgttgacag agaggacgac
     1021 aagccaaaaa gtcaatggat aacaaatgat gattgggcat ggtttgtagg ggaaaacaga
     1081 gagccgatac gcttaaccac aaagccagag ccttacactt tacaaaaagc gttgcattgg
     1141 ctacaaagac aagtcgcacc aaccataaaa atggtacagg cattagacag agaaaatcgt
     1201 acaacgatac tgaaagatat gattgagcag gcagaactaa aagacaagca taaacattta
     1261 ttacaattag agaaatcaac catagaagaa cgtatagata ccgctgttcc acaagagaat
     1321 gacggtattt tttaatgtaa ttgaaaaatt tcagatttag tcagcaaaga gctgttttca
     1381 ttccctatgt gaagtgaaag aagaaaaata aattttttat taaatctgca acaaaatggc
     1441 tacttgcgta cagatatggt gcgagaaaag gaaatctaaa ctttttttga aaataggtca
     1501 ttaaatcggg gcttgctgtc cctatatgaa gtgaaagaag aaaaaagttt tgaaaaaatg
     1561 acggaaattc gtgattagtg gacagtcttc tgtgaaaaga ggaaaaacat catcattttg
     1621 tattcaaaca ggcattagca gttgagtgtt gatgtgaaaa gatgaaaata ctttgttctc
     1681 aacttaacaa tttaaggttt ccgttcccta tatggtgcaa agaaaatatt tcattctatt
     1741 gttggcagta acggtatggt ttgggtagtt agggtgtgaa aaggaaaata ttcttttttc
     1801 atcatcaact tagcaaaatg acattttcta tccctatatg gtgcatgaaa agaagttaaa
     1861 atttttccat ttaaacttag caaatggacg tttgctgtac agatagtagt gaaaaaggag
     1921 aaaatcaacc gttgattgcc aaaatgaacc gttccatttg tactaataat ggaaaaagtt
     1981 ttggattttg gttacgtttc tcctcttttc caacgcgata tataggaaag acgatttttt
     2041 tctttcaatc gttctttgac aactgaataa agctattcaa tacgtttgac agacagctag
     2101 ccgttgaaac aaatacgcca cgacctttcc cctgcaagag cgagcgaaaa cctatttgtg
     2161 ttcttgtaaa agatttgtcc tcttttatcg ccatgacctg tactgtcaga taatgatact
     2221 cccgtacagc cacagcttga gcattaagag cgtcgcacgc aatgggtacg gctacataag
     2281 aactatgcag aggtggaact cctgtgggct atgacaaaag ccgttgaatt gcttagaaag
     2341 attttacaga aagggggtat gtgttattga taatgctgta aaaacaattc aaaagaaaaa
     2401 tggcaagtgt ggcattggca acagaggaaa gacaaagatt gtagtaaaag agcatttttc
     2461 cgaaaagggc aaaacaatgg aagaacttct aactgatgtc atgctggaaa aagcaaagca
     2521 gacaatcgca taaaatcggt gcagttgtaa gaaatagatt gaatgacaaa aagtacccgt
     2581 gttatacttt acttgcaagc aggtattgta aacacgggtt agttataaag gaggataact
     2641 gacaatgaaa caacagattt acaatactgc actttatctt aggttaagcc gagatgatga
     2701 attacagggc gaaagttcca gcattaccac acagagaagt atgttgcgtt tatatgcaaa
     2761 agaacatcat ttgaatgtca ttgatgaata tattgatgac ggctggtcgg gaacaaattt
     2821 tgacagaccg agttttcaaa gaatgattga ggatatagag gcaggaaaaa tcaactgtgt
     2881 tgtaacgaaa gacctttccc gtcttggcag aaattatatt atgacaggac aatatacaga
     2941 attgtatttc cctagtcata atgtccgcta catagcgatt gatgacggtg tagacagcga
     3001 aaaaggcgaa agtgagattg caccatttaa gaacatcatc aatgaatggg tggcaagaga
     3061 tacaagccgt aaagtcaaat cagcatttaa gacaaagttt gcggaggggg ctcattacgg
     3121 tgcttatgct ccgttaggat ataagaaaca ccctgacatc aaaggaaaac tgttggttga
     3181 tgatgaaaca aaatggatta ttgaaaaaat cttttcccta gcctatcaag gttacggaag
     3241 tgcaaaaatc acaaagcagt taagagcaga aaaagttccg acagcgtcat ggctaaattt
     3301 tacaagatac ggtacttttg cacatatctt tgagggaaaa cccgaaagta agcgttatga
     3361 gtggacgatt gctcatgtca aggcgatatt gaaaagtgag gtttatatcg gaaacagtgt
     3421 tcacaatatg cagtctacgg tatcgttcaa gagcaaaaag aaagtgcgta agcccgaaag
     3481 tgaatggttt cgagtagaaa acactcacga gccgattatt gacaaggaag tgttctatcg
     3541 tgtgcaggag cagataaaat caagacgcag acagacaaag gaaaaggcaa cgccgatttt
     3601 tgcagggctt gtcaagtgtg cggattgtgg ttggtctatg agatttggaa cgaataagac
     3661 aaataaaacg ccttacagtt attatgcttg cagttactac gggcagtttg gcaaaggtaa
     3721 ttgttctatg cactacattc gttatgatgt gctgtatcaa gccgtattgg aacgcttgca
     3781 gtattgggct aaggcagtac agcaggacga agaaaaggta ttgaacaaga tacagaaagc
     3841 tggcaatgca gagagaatac gggaaaagaa gaaaaaggca agtacattga agaaagccga
     3901 gaacagacaa aatgagattg accgtttatt tgcgaaaatg tatgaagata gagcctgtga
     3961 gaagataaca gagcgaaatt ttgtgatgtt gtccagcaag taccaaaaag aacagataga
     4021 actggaacag cagataacaa gcctaagaga agaactaagt aaaatggaac aggatatgat
     4081 aggtgctgaa aagtggattg agttaatcaa ggaatattcc gtaccaaagg aactgacagc
     4141 accattatta aatgccatga tagaaaaaat cctcatacat gaagcaacaa cgaatgagga
     4201 taacgaaaga atacaggaaa ttgagatata ttaccgattt attggaagag ttgagtaatc
     4261 aacaagagta atatttttaa ctaagggaaa ccggcggtgt ctatacttgg gtcagtcgca
     4321 cattgggaaa acgattcggt tttgccgcaa tctttttcca atggtttgag ataacagttg
     4381 gctacttgac catgctgtat ttcttaactg gtgcattatc ctatgcaacg ggtattggtg
     4441 ctatacaaaa caataaatat ctgaaattgg caatcctatt gattattttc tgggctattt
     4501 tactctcaca gttaaaagga accaaataca cttcattgat tgctcggatc ggttttgtag
     4561 ctggtatctt gttgccagca ttagtcttgt ttggcttagg gatccattat gtcgcttcag
     4621 gcgctccact acaaacgata ctgtctatga aaacattgat tcctgatttt tcgaaattgc
     4681 ctacgctggt tgtttttgtt tcctttattt tagcatatat gggcgtagaa acatctgcca
     4741 gtcatgcaaa tgaaatggaa aaccctaaaa aggattatcc attagcaatg tttgtattgg
     4801 ttattctggc aatcatttta gatacttttg gcggattgac cgtcgctact acagtacctc
     4861 aacatggttt gtctcttaat acaggggtga ttcaatcgtt agaattccta ttaagacaca
     4921 ttgatccaaa tcttgcatgg attgcgaaaa ttcttgcgat tcttgtttgt ttaggtgtca
     4981 tcggagaaat tgcttcttgg gtaacctctc cttcaaaagc acttcacgta gcagcagaag
     5041 aaggattact tcctgaatat tttgcaaaag aaaacacaca cggtgttcct gctcatttga
     5101 tgatcgcaaa tgggatcgtt gcaacaatct gggcagccgt ctttacatta agcggcgggg
     5161 ggaataatct ttcatttctt gcagcgatgt cattgactgt tgtaatctac ctgatgttgt
     5221 actttatgtt tttcgcgggc tatttgaagc tggtttttaa agatgacagt cttgaaagag
     5281 cctatcaggt tccaggcgga aaagttgtaa aagtaatcgt tgcttgttta ggtcttttga
     5341 cttcactttt tgctttttgt atctcatttg ttccacctgc tagtatcgca gctggacagc
     5401 atgggattta tgaaacgatt ctgattgttt cttatcttat cgtcttagtt attccattta
     5461 cgatctacgc atgccgtcat aaattccagc atgcagaaaa ataaaaaatg aaagctggtt
     5521 attcaaatgg aagtaaaaga attagaacaa ttgattggaa aaaatttcca ctattatcaa
     5581 acaggacaag tggcggatta cattccagca ttagctgaag tggatcccaa acaattaggt
     5641 atggcgattt atgatttgga tacaaaacaa ctgattgagg gaggagacgc cgatgtgcgc
     5701 tttgccatcg aaagcatgtc aaaagttccc gtattgttat tagcaatcaa agacaacgga
     5761 atagaaaaag tctttcaaac aatcaacacg gaaccaaccg gttttgcctt caattctatc
     5821 atgaatatgg aaatcaatca tcgcaaacat ccaatgaatc cttttgttaa tgcaggagcg
     5881 attgcaacaa cctcgttgat tgccggtaaa gatgcagatg aaaaatttga gcgaatcttg
     5941 tcatttatga aagagatctg tgatgatcct aatatcacat tgaatgaaga aatctatcat
     6001 tctgaatcaa gaacaggtga tatcaatcgc tctcttgctt actatatgaa aggtaatcaa
     6061 atgatcgaag gagacgtacc ggagatactc gatgcttatt tcagacaatg ctccctcaat
     6121 gtcaccgcta caggcatagc aaaattagct gctgtgctgg caaataaagg aattgctcct
     6181 tggaatggca aacaaatcat tactgaagaa agtgctacaa tcgtcaaatc aattatgaca
     6241 actgctggtt tgtatgatga atcaggagag ttttccgtcc atgttggtgt acctgcaaaa
     6301 agcggtgtag gcggcgggtt gatggcagct gtgccaaatc gttacggcat aggtgtcttc
     6361 agcccggcac tagatccatt tggtaacagc gcagctggta tccaattgtt gaaagatgta
     6421 gtcaaagaac tagacgctga catctttgaa taaccattct tgaaagaggc tggggtaaaa
     6481 gtccaaacgg ctcaaagcta cctacaatga ggctgaaatt gtgagcttgt ttgacttttg
     6541 tcccagtttc tttttttatt ggtttttgaa agggcttttc taaataaaaa ggaaacaaaa
     6601 atacgattta cagatatgat taaataaaat cataagaaaa actggaggcg tgtatcagga
     6661 atgctggaaa attacatcga gcgaaatata tttagaaaag tgtatctgtg tgaacaatta
     6721 tttgaattcc aagaaataga catcgaacaa acagctattt ctctaagggt gactacacca
     6781 acgatccttc acgatctaga aagtctagcc gagtgtctag aatattgtat aaaagaacaa
     6841 gtaagagaga agcataagta taaactcgta ttcaaacatg gtattgctct ttctgagctc
     6901 acacagtttc tgtacggtca atcttatttt ttaaagtttt taagttatta ttttaatggt
     6961 atattcacga gtacagaact ggctgatctt gaatttattt ctttgagtaa agtatataca
     7021 atcaaaaaaa tcgtattgga tttttttaaa gaaaatgatt atttgaaaaa taaagaaatc
     7081 attatcccag aatttgactc gcggaacatc ttgctagctt tagtacgtta tgtcaattgg
     7141 gaaggctatg aaaataaaaa tccgtatgta gaagaagctt gtaacgagtt gattgattat
     7201 gtagagactc atttttttaa aagaagatat tcggaagatg aaaagtcctt tatcatacga
     7261 gaaatagaga tcgcaatcgg aagaaaggca tttccccttg gactgaaaga agaagataag
     7321 aaaagcgcag aaacgaagcc gttatttcaa ttagtctgtc aaggattgga agagcaaaaa
     7381 ggaattctag acttacaaga agaagatgtc tattatattt tttctttgtt caacagtaga
     7441 aattatacca atgagaacat ggagctgtta agaaaagatt tcgaagttgt ttataaaaat
     7501 tttgttctca atgacctatc actgaatgaa ttgattttac agattattag tatcgctgaa
     7561 atcgacgatg cagaaaattt tattttcaag caatcctttt tacaatttgt ccgtacgttg
     7621 tgggcagatg gacaagtatt tttaccggaa aggatctatc ttctatcaga aaaagaacaa
     7681 cttctgtacg gaaaaattgt agaaatattg gaagagtgga aaaagaaaaa tcaaatccaa
     7741 ctaagatgga atgaaaattt cattcgaaaa ttcgtcaaaa gtctttcttt gatcaacagt
     7801 gaacaagcgg ggagacagga aatagaaatt tttgttgttt cagaaagttc agtcaaacaa
     7861 tttttttatc gaacccaatt tcatttgtat gcggaagaag gcatcgctga aataaatcct
     7921 attttatttc gttcattaga agaattgcct gatgaatgtc tatgtgcgaa aaaaagagtg
     7981 gttttgtgcg atgctgctag ctatcaaaat ggattagaaa ctgaaaagac aaagatctat
     8041 ccaatctcat taaaaggtgc aaacatccat ttgtatcata ttgctcagga acttcaccta
     8101 ttaaaaaaac atatttcttg atgagacctt aattaggaga ccttctacaa agctttttaa
     8161 ttgtctgtag aaggttttta tttgagtcct gattcgtgcg aagatctact tatataacaa
     8221 gctttctctg aaattttttt aaaatgctat tgacttagag tcaactctaa gtagtagaat
     8281 agataaagtg taacaaatct attttcatta aaagaagata gtacggagga gagaatgtat
     8341 tatgagtaca gtattacatg tattggatgc aatcaatagc cccgcagatg tcaaaaaatt
     8401 atctgttgag gaaatgaaac agttggcaga agagatgcgc caaaaaatct tgcaaaagga
     8461 cagcgatgta ggtgggcacg ttggtccgaa tctaggtgta gtagaattaa cgattgcgtt
     8521 tcattatgta tttgattcac caaaggataa aatcgtttgg gatgtttccc atcagtctta
     8581 tggtcataag cttttaactg gtcgaaaaaa tggttttgaa aaaggacatt accgtgaagt
     8641 gagcggatat tccaaccaac atgaaagtaa acatgactat tttacagtgg ggcacacatc
     8701 tacatctatc agcttagcaa caggtttagc taaagcgaga gatctgaaga aagaatcagg
     8761 aaatgtggta gcatttatcg gtgacggctc cctaagtggt ggcttagctt ttgaaggatt
     8821 gaacaatggt ggtgttttac aatccaatct aatcgtatta gtaaacgata atgagatgtc
     8881 tattgatgag aatcatggca gtttatataa aaacttagct gagcttcgtc aaaccaacgg
     8941 taaaagcagc aataacctat ttaaagcaat gggatttgat tatcgttacg tggataatgg
     9001 aaacgatctg gaaacggtga tccgtttatt tgaagaagta aaagatattg atcatccgat
     9061 tgtacttcac gtccatacgg aaaaaggtcg cggttatcag ccggcaatcg ataataaaat
     9121 gaaatatcat tggcatgtac catttgatct gtccaccggg gaggcaaagc aagtaacttc
     9181 tggagaaaca tttaatatga tcatcatgga ttatttggat aagaaaatgc aaacaagtac
     9241 agctcctatt gccgcagtca atgcagcgat accaggcgcc tttggactga aaacattcca
     9301 agaaaaacat ccagatcatt attttgacgt ggggatcgct gaacagcact ctatcacttt
     9361 cgcttctgct ttggcttctc agggagtacg tccagtcgtc ttccaatcaa gtactttctt
     9421 gcaacgggca tatgatcaat tatcacatga tttagcgatc aacgagaatc cagtgattat
     9481 tttagtcggt ggcggaacga tctcagctag tgaccgaaca caccaaggaa catttgacat
     9541 tccttactta tcaagtatcc cgaacatcaa atatcttgct ccagcaacaa aagaggatat
     9601 gatctctatg ctagattggg cgttcgaaca aacagcgcag cctgtagcaa tccgaattcc
     9661 tacacatggt gtagaacatg gtacagctat cgcaaatgat tatagtattc cagcctatca
     9721 aacagtaaat aatggaggaa aagtagcaat tttagcttta ggcggttttc ttgaattagg
     9781 aaaagaaaca gtaaaagctt ttagaaaaaa aggaatcgaa gcaacgttga tcaatccagg
     9841 aacgatcaca gaaaatgacg tagaaacatt agataaatta aaagaaacac acgaattagt
     9901 catcacatta gaagatggta gtttgtcagg aggatttggc gaaaatatca gtcgttatta
     9961 tggtgattct tcaatgaacg tattaaattt tggtgcagag aaagaattca cggatagcgt
    10021 tcctgtaggg cagctttatg aacgttatca tttaacacca aaacaaatcg ttgaagatag
    10081 tctgcgtgtc ttgaatattc aataattcat tatgctcacc atccagataa ttgtttatac
    10141 tagtatctgg atggtttttt cattataaaa agcaaagggg tggacgttat gtatcagcaa
    10201 gtagaagaat atgtcaaaaa gttccaagaa aaatatccta gttatcaatc agaaaaatta
    10261 ggtttctttt catttgggga tacagaaaaa atggcagacg agttgagcga actagttgta
    10321 aaaaagataa agactggtac atcaagtggt tacgatctgt ataaacaaga gaatgagcct
    10381 ttaccaaaag ttggtcaatt agacgtggtc ctgaatggaa aaaatgaacc agtttgtatc
    10441 atcaaaaata taaatgtgac catcttgcct tttagtgaag tcacgaaaga gcatgcttgg
    10501 aaagaaggag aaggggatcg gacattagct tattggcgga aagtccatgc tgagtttttt
    10561 aaacatgctt ataaagaagc gttgaacata gaatttacag aagagaagct ggtcgtatac
    10621 gaagaatttg aagtaatttt cttttagtaa gaaaggtctg ctgaaagaaa tagctcatta
    10681 ttgtctcctg aatagaaaag ggttacattt ttatatgaag ctgtattttt atataacatt
    10741 actggaggag atattgtgga tacactggaa caaatcaaag gcggagtcgt cgtttcttgt
    10801 caagctttgg aaaacgagcc gctacacagt tcttttatta tggggagaat ggctttggct
    10861 gcaaaagaag gcggagcagt tggtattcga gcaaatacga cagaagatat caatgaaatc
    10921 aagcgaatcg ttgatttacc ggtaataggg attatcaaaa gagattacga tgggtcagaa
    10981 gtgtttatta ctgcgacaga aaaagaagtg gaagaacttt tggagacgca agcagaaatc
    11041 attgcactgg atgcaacttc tcgtaaacgt ccgacagaaa aaacaactgc tgaacttgtc
    11101 caaatgattc acgaacacgg tcggttagct atggcagata tttcgacttt tgaggaagcg
    11161 cttcaagcgc aagaagatgg ttttgatctt ttgtctacga ctttagcagg ctatacagaa
    11221 tattcacgaa agacgcagac acctgatttt gatttattga atcaaatcat agaacaagtc
    11281 gatatcccag taattatgga aggacatact gactctcctg aacaagtaga gaaagcatat
    11341 gaattaggtg ctttttcagt cgtagttgga tctattatca cccgtccgca attgatcact
    11401 aaaagatatg tcgaagccgc aagtaaagca aataactaaa aaacacgcac ggcacagatt
    11461 cttgacaaaa aagaaaaatt gatagatagt ttaaagcaga ttctaagaag atgaaaaaag
    11521 cattactttt cattttgtct tcaagatctg ctttttaacg aattttatag aaagaaggcg
    11581 aaaccatgtt tgaacggagt ccttcagaat tgacagaacg tgaaaattac aagttgctga
    11641 ttggttcagt catccctaga ccagtagcag tagtaacgac taaatcagca aaagatatag
    11701 tgaatatcgc acccttcagt tacttcaata tcgtcagttc gaatcgaccc atcttatcct
    11761 tggcgatcca gagaaaacaa gatgcagtaa aagacacagc aaagaatatt cttgagacaa
    11821 gagaagctgt tatccatatc cttgatgagg acaacgtaga agaagtaaat aaaacagcag
    11881 ccaatctgcc taatgaggaa agcgaactgt acctgactga cttgacttta actgattcag
    11941 taatcatttc agtaccagca ttgacagaag caaaagttcg ttttgaaaca tccttatatg
    12001 aacatgtcga gatacggaat aaaaataaag taacagctga tttatttttg ctagaagtac
    12061 agaaatatca tttaaatgaa acagtctatg atgataagac aggaaggatc gatccaagaa
    12121 aattgaatgc tgtaagccgt ttggcaggaa atgattatgc agcaatagga gaaattttca
    12181 cgattgcacg tcccgaataa aaaggagcac tagttatgtt aaaaataagc tataaaccat
    12241 cgacagattc aaaagaaatg aaaaaagaat atgaaacagt gaatgatttt ctgcaagggc
    12301 agtacttaga agttccgcca ttgcaagacc attttgtcgt tactactgtt actttagatg
    12361 gtaaggaaat agaaatgcca gatcaaacga ttagcggatt gtttaattac tttaacaaat
    12421 aaatatttat tccgaaaagt gaaaaaacat ataaaaaaac acataaaact aaaaaattat
    12481 caaagaatcc tggtgcaact gaaagaattt gcaacgggat tctttcaact tatttgttat
    12541 ttttttctaa aaaatattaa agattgttaa atttcactat ttcatttcaa attccattgc
    12601 ctaatcatta ggaaaagtct atcatgaact cgtaaataga atatagtaat aaccttgtta
    12661 ggtgaggctc ctatacaaac ataggctgtt gcccagaaac gtcgaaagtc gccaatgggt
    12721 aaaacagaaa ctgttggatt aaggcttttt ccaatagcta agaatgtttt ctttacgttg
    12781 tatagtgcta aaactcggac gatgagatga tgctaggtca gaattcttgt tcgaaaaggt
    12841 ttctgacctt ttctttatat ttttgtgact gacttatttg atgagacaaa ctcgctcctc
    12901 acctgacaag tggattgctg tgtgaggagg agatgcggtg aaaaaagatc gattggtgga
    12961 ttttgggtcc ttatttgact ttgtccatga tcggtctttt agaagtatac agtgccagtt
    13021 cttatcgttt attgcaagcc gatgaaaata caaaaagtct attattgcgt caactaattt
    13081 tcatattttt gagttggagc gtgatcttct tagctcgttc agtcaaacta cactatttac
    13141 ttcaccctaa aatagcagga tacggtttag ccttatcgat tttcttttta gtattagtaa
    13201 gaatagggat attcggtgtc actgtcaacg gcgcacaacg ttggatctct ctgtttggca
    13261 ttcaattcca gccttctgaa ctggcaaatc tttttttgat tttttattta agctggtttt
    13321 ttcgtgacgg aaatagtagc ccaaaagatc taaaaaaacc attcctgatt acagtaggta
    13381 taacttttct gattttattt cagccaaaga ttgctggagc attgatgatc ctttcgattg
    13441 cgtgggtcat attttgggca gcggcggttc catttaaaaa agggatctat ctaatcgtta
    13501 ctttttctgc attgctgatt ggagcagcag gcggggtatt atatttagga aataaaggtt
    13561 ggcttccaca gatgtttaat catgcttatg aaaggatcgc gactttaaga gattctttta
    13621 tcgatagtca tggtgcgggc tatcaaatga cccattcgtt ttatgcacta tataatggtg
    13681 gcatatttgg gcggggatta ggaaatagta tcacaaaaaa agggtatctt ccagagacag
    13741 aaacagattt tattttttcg attattacag aagagcttgg gctaatcggt gcattgtgtg
    13801 tgcttttctt attgttctca ttatgcatga gaatcttttg tttaagcagt cgctgtaaaa
    13861 atcagcaagc tggattgttt cttttaggat tcggaacttt gctattcgtt cagacgataa
    13921 tgaacgtggg aagtatcgcg gggctgatgc caatgactgg tgtgccattg ccatttgtta
    13981 gctacggagg aacaagttat ctgatccttt ctttaggaat cggaatcacg ttgaatattt
    14041 cttcaaagat acaggccgaa gagttggcgc tctatcgacc tgaaaaacaa taaagagaaa
    14101 ctgagaaact ctcagtttga attatcataa agtacaatct tttatttttc aagcgagagg
    14161 ttcttttaga aagaggaatt cgcaatgaaa gtatctcaaa aaatcatatt atctattttg
    14221 ttactgctta cattagtagt tggctatttt tcgattgagt ttatccatgg attttcgtct
    14281 gcaaaacaga gttcaactgt gaaaaaggta gagccgaaaa gtatccctac cacactaaat
    14341 gtggctttga ttggttcaga tgctcgttcg aaagacgaaa acggtcgctc ggattcactt
    14401 ctggttgcac aatacaacca gaaaacacaa caagcaaaac tgatctctat catgagagat
    14461 tcatatgtcg atattccagg ttacggaatg aataagataa atgcagctta ctcttatggg
    14521 ggagttgatt tattaaatca aacattaaag gaaaatttca aatttgaagc tccctattat
    14581 gcaagtatca catttcaaga ttttatcgat tgcgttaatg aactgtttcc tggtggagtc
    14641 aagatcgatg cagaaaaatc tctagattta gatggcgtat atataaacaa aggagagcaa
    14701 gtgatggatg gtaatacttt actccagtat gctcgatttc gggaagacaa agaaggagac
    14761 ttcgggagga tcagaaggca gcagcaagtg atcaaagcta tctctcagca gctgaaagac
    14821 gtaacttcaa tattcaagct acccaaagcg gttgggaaat tacttggtag tatccaaacg
    14881 aaccttccag aaagtgtgct tctggattgt ggtatggatt tcctcaaaga catatgttag
    14941 aataaacagg tataaatagt gaaataaagg aatgacaagc aagagaagga ggaaaaaatg
    15001 aaaagaagtg acaagcacgg caaaaatcga acaggcgctt atattgccgg cgcagtgatc
    15061 ttaatagcag ctgcgggtgg cggttatttt tactaccagc actatcaaga aacccaagca
    15121 gtagaagctg gagaaaagac agttgagcaa tttgtccaag ctttgaacaa gggagattat
    15181 aacaaagctg cagaaatgac atcaaaaaag gcggcaaata aaagtgcatt atctgaaaaa
    15241 gagatcttag ataaatacca gaatatatac ggtgctgctg atgtcaaagg acttcagata
    15301 tcgaatctaa aagtagataa aaaagacgat tctacttaca gtttttcata taaagcaaag
    15361 atgaatacct cattaggtga attgaaagat ctttcttata aaggaacatt agacagaaat
    15421 gatggtcaaa ccacgatcaa ctggcaaccc aacttggttt ttccagaaat ggaaggaaat
    15481 gacaaagtaa gcctgaccac acaagaagca gcaagaggga atattataga tcgaaatggt
    15541 gaaccattag caacaacagg caaactaaaa caattaggag tcgttccaag taaacttgga
    15601 gatggaggcg aaaaaacagc caatatcaaa gcgattgctt cctccttcga cttaacagaa
    15661 gatgctatca atcaggcgat ttcacaaagc tgggtacaac ccgattactt tgtcccattg
    15721 aaaatcattg atggagcaac gccagaactt ccagctggag ctaccattca agaagtagac
    15781 ggcagatatt atcctttggg tgaagcagct gctcagctga ttggttacgt gggagatatc
    15841 acagcagaag atattgataa gaatccagaa ttaagcagta atggaaaaat cggtcgctca
    15901 ggtttggaga tggcttttga taaggatctt cgtgggacaa caggtgggaa attaagcatt
    15961 acagatgcag acggtgtaga gaaaaaagtt ctgatcgaac atgaagttca aaatggcaaa
    16021 gatatcaaat tgacaatcga tgcgaaggca caaaaaacag cttttgacag tctaggagga
    16081 aaagctggat caactgttgc gacaacgcca aaaaccggtg atcttcttgc gcttgctagc
    16141 tctccaagct atgatccaaa caaaatgaca aacgggatct cacaagaaga ttacaaagct
    16201 tatgaagaaa atcctgaaca tccattcatc agccgatttg cgacaggtta tgctcctgga
    16261 tctacgttta aaatgattac agcagcaatc ggtctcgaca acggcactat cgatccaaat
    16321 gaagtgttga cgatcaacgg gcttaaatgg caaaaagaca gttcttgggg atcttatcaa
    16381 gcaacgcgtg tcagtagtga tgtatcacaa gtagacttaa aaactgcttt gatctattcc
    16441 gataatatat atgcggcaca agaaacgttg aaaatgggtg agaaaaaatt tcgtacaggc
    16501 ttagataaat tcatttttgg tgaagacctt gatttgccaa ttagtatgaa tccagcacaa
    16561 atttctaatg aagatagctt taactcagat atcttgctag ctgatactgg atatggacag
    16621 ggcgaacttc taattactcc tatccagcaa gcagcaatgt attctgtttt taccaacaat
    16681 ggcacacttg tctatcctaa attgattgca gataaagaga caaaagataa gaagaatgtc
    16741 atcagcgaaa cagcagtaca aacgatcgtg ccagatctga gagaagttgt gcaagatgta
    16801 aatggtacag cacattctct ttctgcttta gggattccgt tggcagcgaa aactggtaca
    16861 gcggaaatca aagaaaaaca ggatgtaaag gggcaagaga acagtttctt gtttgctttc
    16921 aatcttgata accaaggtta tatgatggtc agcatgttgg aaaataaaga agatgatgat
    16981 tcagcaacca aacgagcatc ggaactattg caatacctca accaaaatta tcaataaaaa
    17041 attgcttttt aagtacagta aaaggactgt ggcgttatcg tcacagtcct tttttcaaat
    17101 aaacggtttt cacgatgtct ctttcacaac ttctttttga tttactcaca cttcataaac
    17161 tttttttgta aaatatttga gaacaagtgg agaaataagt gtacttaata aaatcacgat
    17221 aactaaaggt gaatagtact gtggttcgat caattgactt tgctgtccaa tttgtaaaat
    17281 gatcaaagcc atttctccac gcgaaatcat accagcaccg accatcaatg aactatgttg
    17341 agaaaaaccg gcaatctgag caccaaaata acctccaagc aatttggtca aaatcgctac
    17401 aagcgtcaat atagaaataa agagtagttg ttcactaaag tcagaaaaat caacttctaa
    17461 tcctacactg acaaaaaata ctggtataaa tactgcatat cctaaagcct ctacattgtg
    17521 gtatacttct tctttaacct ttgtctgact gacagctatc ccagcaaaaa aagcaccaat
    17581 gacagagcta agcccaacaa gatccgctac gtaagacatt cctaaacaaa taactagcga
    17641 tacgatgata atagaagaat ttgcgtacac tttttctgca aatgaaagta ggaaaggcgc
    17701 aatccatttg actaataaga aaataaaaac aaaataaatt agctgttcaa tcaaaacaag
    17761 cggtaaagaa gcattgctag aagcttcccc cgttagaaaa gataggctga aacttagaac
    17821 taaaacgact aaaatgtcat ctaccacaga agcacccaaa attgttgatc cttcttttgt
    17881 attcactacg tttagttctt ttaatacttc tactgaaata ctgacagaag ttgccgcaag
    17941 aataatccca aagaaaatcg cttcatttcc cgaaacttgg aatgctttgc ctgtcaacca
    18001 gccaaaaaca accgggaaga tgatcccaag caaagcaacg aacataccag gtcggaaata
    18061 ttttttcaaa agagataaat cactttccag accagctaaa aacattaata agatcacacc
    18121 aatctccgaa aagtcatgga ctagaatatc aggtttgacc caattcaatc cagcttggcc
    18181 tagcaaaaca ccaacaagta attgtccaat cactgctggg atacctaatc tccgcgagat
    18241 atgtgagcca atcgttgttg aaatcaatat caggcataaa ataccaataa attccattct
    18301 catcattcct ttgtataaaa tatagcccga acttagcata acatacatta ttgaaaaaat
    18361 aggaaaatat actcacaaaa aaaccaacaa tccaaaagat ttttttggat tgttggtttt
    18421 tatacggtct attttaataa ctaaaaatga ttaacgtata gtcatttttc ttattctgat
    18481 gaaattgata atctcctgga tgcccatgaa aatcaaaata aagccgaata tttggaatag
    18541 aagcatcaaa cttttgaatg gattgaacaa taagacgaat ccgccaatca acaacagaat
    18601 actactataa acactccaac ttttagcttt tgtattcatg ctgaagaaca actggaacaa
    18661 gctgttgatg ataacagcta atccaagaag aatcggcagg atagaaacga ttgcaggtgc
    18721 aaaagcatag acgatcagag ctaagatcag gaaaaaaaga ccactgaaca gaccgatact
    18781 ccaagaacct gttttccttt taaaacggta agcctcaaaa agattcaaca acccaagcaa
    18841 aacaagatag gcggtgatca gataaccgat aaaatgaaaa acagcagaag ggtttagcac
    18901 aatcgcaatc ccagaaataa tataaacagc agctcttaat aaggcgtatt tttgaatatt
    18961 ttgtttaatc gtctcaaaca tataagaaaa cctcctttga cttattttaa cgtgaactag
    19021 tcattttacc aaattaaatg ttacaaaatg agcgaaggag aagaatatct ctggaaaaat
    19081 caacttctga ggatcttttc catttttttg ccttttgcta gctcatcgat cattttatct
    19141 aaataacgaa tttctttcat caagttatcc tcgatgtctt ctactcgtac accacaaaca
    19201 acacctgtaa tcaaggaacg tgaaggattg gggacagggg cttccgcaaa aaattgttcg
    19261 aaagtcactt gtttttgtag ttgatattct agttctgttt gggaataacc cgtcagccaa
    19321 caaatgattt catctacttc ctcttttata cgatcttttc tctccgcttt tgctacatac
    19381 ataggataaa cgcttgcaaa acttgtcgta aaaatacgat tttgcgccat aatcagtcac
    19441 ctttcttttt ttcaaattat ttactactta tagtatataa gaaaaagaga agaaaaagcg
    19501 attattcaaa aaaacaaaaa ggattgtgac aaaattcttg tcaccatcct tttttccgct
    19561 ctctgacttt ttacaatctt catagctcaa gtgcgcatca gataatcgaa tgctcctaaa
    19621 gctgcagtag ctcctgaacc cattgaaaca atgatttgtt tgtatggact atctgtacaa
    19681 tcaccagctg cgaaaacgcc ggggatattc gttgcgccat ttttctcggt cactatttca
    19741 tttcttggat tcatttcgat cgttccatct agccaatccg tattagggag taagccgact
    19801 agaatgaata ctcctgaaac agctagtgta tgcatcacca tcgtacggcg atcgatatag
    19861 gagagagatt ctacctgatt cgtaccgctg atttctttcg tcgctgcatg tttgataaca
    19921 gtgacattcg caagagagac tagtttatcc tgtaagattt gatctgcttt taattctggt
    19981 aagaattcta gaacatatac atgttttgct aacccagcca aatcgattgc tgcttcaaca
    20041 ccagaattac caccaccgat aacagcaatc tcttttcctt taaataaagg gccgtcacag
    20101 tgtgggcaat aagcaattcc tttattttta aactcttttt cgcctggtac attgatagaa
    20161 cgccagtgcg cacctgtcgc gatgatggcg gtttttgctt taagtgatat accgttagct
    20221 aagccaatat taataaaatg ttgctcttta tttaaggaaa cggcctgttg attcatcata
    20281 atgtcgatag ggtaactacg cacatgactt tctacttgct gcatcaattt tggaccttct
    20341 gtgtaagagg taccaatcat attttcaatc cctagtgttt caacgacttg tccgccgaaa
    20401 gaatcagcga caagtcctgt acggattcct ttacgtgctg catagatagc tgcactacta
    20461 gctgctggtc caccgccaat cactaataca tcataaggtt ctttttcaaa caactcttct
    20521 tcaatcaaag ggccagtgat tttttcaaca atttgttcga tcgtcatgcg tccgctacta
    20581 aattcttctc catttaaaaa cacagttggc actgccatga tttttttatc atcgatttct
    20641 ttttggaaca tcccaccttc aatcatcgta tgagtaatat ttggatttaa tacagctaat
    20701 atattcaatg cctgtacaac gtcggggcaa ttatgacaag tcaagctaac ataactgtca
    20761 aaatgcaatg tttgtgttat ttttttgatt ttaagactca aagattcact gattttcggt
    20821 gcccgtccac taatttgtaa catagccagt aaaaaagagg aaaattcatg accgaggggg
    20881 ataccagcaa aaacgattcc actttctttg ccaactggat tgatagaaaa actaggtgtg
    20941 cgcatcgaat ctcttttttc ccaagagatt ttaggagaca tttctgctat ttccttcgta
    21001 aattcagcta gtttttgact attttcacta ttatccaaac taacagaaaa aacaatagga
    21061 gattcaatca gtgctaaata ttgttcgaga ttttgtttag tttctttgtc taacatatcc
    21121 accacctaaa tctttccaac aagatcaaaa cttggtttca atgtttcgcc actttctttc
    21181 cattttgccg gacaaacttc acctgggtgg gtacgtacat attgagcagc gcgaatctta
    21241 tcaatcagtg cactagcgtc acgaccgatt ccatctgcat tgatttccat agcttgaacg
    21301 attccgtctg gatcaataat aaatgttcct cgctgagcta atccttgctc ttcatccaaa
    21361 acatcaaaaa tacgagaaat ctgatgagac ggatcaccga tcatcacgta ttcgatttta
    21421 ccgatagcat ctgatgtatc atgccatgct ttgtgagtaa agtgggtatc ggtagagcaa
    21481 gaaaagactt caacacctaa agatttcaat gtactgtatt gttcttgtaa gtcttctaat
    21541 tcagtaggac aaacaaaagt aaaatcagct ggatagaagc aaagaatact ccagttcccc
    21601 ataagatctt tttcactgac ttctaaaaat tctccttgat gataagcatg tgcattaaat
    21661 tcttcgattt ttttcccaat caatgacata gtaaaacctc caataataat tatttattta
    21721 taattattgt aatttagttg gagttgcaaa gtcaatggat attttgttat ttaataaaga
    21781 ttactcaaaa acgaaggtta attataaaaa tagaagaggt cgcaagaaaa tgctactgct
    21841 ggaaatactc gggataacgt tgagtaacag tttcctgttc tttcagcatc atgtgtaagt
    21901 gcaatcccgt ttgaaatcga gcttcttctg gttttttatc aactaaagcc ttgagtaaat
    21961 cataatgttg ttccatgatc gtttcccatg gaagcccctg aatggtcact cgtaaccagc
    22021 ggaaacgttc taaatgggta ttatggtcat ctaaccagtt ccaaacttct tttcttcctg
    22081 caatttcgaa acaagcttca tgaaataaat tatctgcacg gaaaaaagcg cgttcgtcat
    22141 ttattttgac tgctttttct tgttcttcta aaatcgtttc tagcactatt cgtgtttgtg
    22201 tgtccaattt ggaacaacat tcaatcatga tttgctcttc caactgttct cggacaaagc
    22261 gagcattttc tgcagaaaca agattaattt tggaaacata cgtaccactt tgaggtatcg
    22321 tatacacgag ttcttgctga cgtagttgaa tcaatgcttc gcgaatggga gtccggccga
    22381 tagcaagatg ttcctccagt gccttttcag atattttcct tccaggttct aagtcagcat
    22441 agataatttg tttacgaatc gcttgataag cttgtgattg caagctgctt ttcatcttta
    22501 ttcctccaaa atcatttcta tgaacgtagt ataacagaag aggtctgaaa aacaatctgg
    22561 tatgtcacat taactagtaa atttgtataa atagtaaaat tttggttgac atagcgattt
    22621 ttattgcata aaattcactt gaatacaaaa actaatatgt tagtttagga ggggaaaaat
    22681 gattgattta aacaactgga ggaactttat ccctgaatta tctcaaaaag aagtagaaat
    22741 acctcatttt gaccgtgcat cattaagaga aaatggacaa aaaaatcctg tctggatcca
    22801 tttcggtggc ggaaatcttt atcgaggatt tcatgcagaa atcgctcaac gtttagcgga
    22861 tctaggcgaa ttgtccactg gtgtcattgt ttgtgaaaca ttcgatgaag aaataattga
    22921 aagggcttac caagattttg atgatttgat tcttgaagtg gtcatgccaa gcagtggaga
    22981 gttcgtcaaa cgtatattag ctgcgacagc tgacagtctg tattgtcatc cagattgttc
    23041 tgaacacttt gataagatga ttcgacactt tgaaaatcct agtttacaat tagttacttt
    23101 tgctattact gaaaaaggtt atcgattgaa agatacaaat aataaatata taggaccgat
    23161 tgcagtcgat ataaaaaatg gtccaaagaa gccatcacat acaatgagta ttcttgctgt
    23221 gctactttat aaacgttttt tatctggagc ttggccgatt gcacttgtct ctacagataa
    23281 tttttcacaa aacggtagtc aattacgtac ttctttgcta acgatcgcaa aagaatggca
    23341 aaaggaaggt tatgtacaaa atacatttat ccaatactta tcgaatgaag aaattgttgc
    23401 gtttccatgg acgatgattg atcgtatcac cccgaatcct tccttgtcag ttagcaaaat
    23461 gttggcaaaa gagggctttt caaatatgtc gattttccaa accgcaaaag gaaataatat
    23521 tgctccattt gtcaatacag aggacatcca ttatttaatc atggaagatc attttcccaa
    23581 tggacgtccg aaattcgaaa aagctggggt attcttgact gatcgtgcaa cggtagataa
    23641 agcagatacg atgaaggtga cgacttgcct taatcctttg catacgtctt tagctattat
    23701 gggttgttta ctggggtata ggagcatagc tgaggagatg aaagatccag atttatcgct
    23761 gctagtttct gaaatcgggt acaatgaagg cctcccagtt gtggagtctc caggtatcat
    23821 tgatccaaag atctttctgg aagaagtaat tactgtgcgc ttttctaatc caaatattcc
    23881 taatatgcct cagcgaatcg ccacggatac ctctcaaaag gtggcgattc gttatggtga
    23941 gacgattaaa aaatatatgg aaatagaagt taaagacccg cagacacttg tattcatacc
    24001 attgactctc gctgcttggc ttcgatacct tattggcatt gatgatcaag gaaaaccatt
    24061 catcccgagt ccagacccac tattgacaga ccttcagcat aagctttccg atattcaact
    24121 gggtacgcaa gatacatcac atattcatga gaaaatggct cctattttgt caaataaaga
    24181 aatttttggt gtagatcttt accaagcaga gcttggcaga aaaatagaag aaatgttcat
    24241 caagatggta gcaggaccaa atgccgtacg aaaaacatta caggattact tacaagaaca
    24301 tggaggaaat ttatcatgga aatgactttt agatggtacg gatcacagga agaaaagatt
    24361 accttagatg aaatcagaca gattcctgcg gtcaaaggga ttgtaggcac attgatggat
    24421 ataccagtag gagaggtatg gcctagggag aggatcaaag aactaaaaga agaaattgag
    24481 tctcgtgggt taacactaaa agtaattgaa agcgtcaatg tgcatgatgc cattaaaatt
    24541 ggattacctt cccgtgagca gtatatcgaa aactataagc agacaatccg aaacttggca
    24601 gaatatggaa tagaagtcat ctgctataat tttatgcctg tatttgactg ggtgaaatct
    24661 gaccttgact accgcttaga agacggctca tccacgttag cttttatatc tgatgatatt
    24721 ccagatgatc caagagaaat cgtcgcgcgc atcgaacatt ccagcaatga atttggattg
    24781 ccgggatggg aacctgagcg cttagctcct atcaaatcct tgcttgaagc ctatgcatca
    24841 gtagacgaag aaaaacttcg agaaaatttt gcctattttc tccaatcaat catcccaaca
    24901 tgtgaagagg taggggtcaa aatggctgta catccagatg atccgccata ctcgattttt
    24961 ggattagctc gaatcgtaaa gaatcgagaa gatttagatt ggttgtgtcg catagtagac
    25021 agcccttata acggaatcac tctttgtaca gggagtattg cagaagatcc agaaaatgat
    25081 gtatatggga ttttagcaga attttgccaa agagatcgaa tcccatttgc acatgttcga
    25141 aatattaaat ttttagctaa tcgagatttc tatgaggcac cacataaatc cgaatacggt
    25201 tcattagaca tgtaccgcat actaaaaaca atgtatgaca acggatttaa cggatacatc
    25261 cgtcctgatc atggaagaat gatctggaat gagacaggaa gaccaggata cggcttatat
    25321 gatcgagcat taggtgctgt ttatctaaat ggattatggg aagcaatcga aaaaggaaac
    25381 aaaagtaaag ataattagtg aaaaaaactg taattttatt atgtaaataa gaaggatgtt
    25441 caaattcttg atacataaat ccggaggttg ttaataagca ttcggattta tgtatcttat
    25501 ttggtctagg aagtaacttg attttctttt ttcgattgat ccatgagtgc ttcttctttt
    25561 aattcaaaga tatattctga gatcctcata atcacttttt tccagtcttc tggtgtgatg
    25621 attcctttta aaatatagat ttttttgata tgagaaggca attgtttcaa agggcagtcg
    25681 ctgataacga tgttgcagtt ctccataaaa tcattttgta ttttgatcga ttcatttgaa
    25741 aaaatttgtt ctatcttaac gatcagatct ttactgatat aaaaatcacg cgtatagttc
    25801 acaccaatca caattcgcgg ctgttttttg tagcgatcat acaaatgtac taggtcttca
    25861 accatccatt gcacattttc cttttctaaa ataactggta acaagtgttg attttgttct
    25921 ctgaaatcca tataaaattg ttcaatggcg gtttggactt ctttcaatac gctatgttct
    25981 tgataaagca tgaaagtagg aaggggcgta tttggttgat tgaagttaag aaatccatta
    26041 taaagtttat ttcgtgcgaa tgccaatgag taaagaatct tttctttttc aggtataaac
    26101 aaatcaaata cagtacaaag ggactccacc atttctcttg aataaagaac atgaggaacg
    26161 tttgcttttt ttaaaagttt gtattgctcc aagcttgttt cttccgattc gattttcgtg
    26221 atagaaagtc gagctaaaat attcactaag atgcgttgtt cgcgacaaat tggcagatta
    26281 ggtcgcaaaa tatctacaga gagatcaata aaaaagtcaa tatattcata ctctgtgaat
    26341 tctttttgta cgtctttcac agaagttgca ggaaaattct ctttacatat ttttaataga
    26401 aaatataatt tatctatcat accgctattt agtcggtcaa gtacttcttt tttaaaaaaa
    26461 gtattcatca taaaagccac tacttgcgga tcttcatcat aaaattctgt agcagtactt
    26521 gaaaagatgt tccaataaat atctaatgct gcatattgaa tttgtacctc ttttccagaa
    26581 aaagactttc tgccattatt agaaaaggag atagacaaac cgtactatgc taaaaatgca
    26641 ttaatctttt tcattttcgt atacaaataa gaggcactat agtttgtttg ctgactgaaa
    26701 ctgataatcg aatttgtctg acagctgaac aaagaagtca agatctgata actgactgat
    26761 tcgttgcagt atcttccaaa tagacgataa tagcagtaag ccggatcaat tccttcaaaa
    26821 ttcaacttta aaaaaggttt ttgcttatca atatgcagtt ctgttagatt gagacgctca
    26881 aagtcttgtt ctaattcttt gatgtatagt aaaaaatttg attcagacat atggaaatga
    26941 tctgctaaat attgtgtagt aataaagaaa cgatgttcag ataggaaaag aaaaatatct
    27001 aattttgtat tgcttttttt cttcaagata aacgttttaa tcgcttcttg agtttgcatg
    27061 aaagaggctc ctttttactt aaattgttaa aacttgaaaa tattactgtt tttttcataa
    27121 atttagacaa aaaaacagta aacatatagg tagaatctct actatcttat ttcttgcata
    27181 caaaaaaatc aaggagtgag agagttatga gatattcaac catcttaata tcaacatttg
    27241 cagccttaag cttatcgctt actataaatc agacagtcca cacagaggag acagaaacag
    27301 ccaaatggac agaaaccaag tgggccaacg tcaatcgaat tacctttcag gatgaatttt
    27361 catccttcaa ttcaggagtc gcaaaattaa gaaaaggaca aacagcgacc atcaaagcaa
    27421 aaattgacta ttcgggagac ttagcaccta tactacaatc agctgttaca tttgagaatg
    27481 ttgatcccac tttgtcgatt ggctatgacg agaacacttt gactatccaa cacaagcaag
    27541 cgatatttac cttcaccgtt acattaaatc aggacttgtt aaaaccagcg ctttttactg
    27601 tcaaggtatc agatggacta ccgaacgatc atcatttaac gccgtatagt cagcggcaga
    27661 caattgagca agattcagcc atcgactcag gaggagatct tgttgaagaa ccaacagata
    27721 aaccagaaaa tgaaaataag ccagaagtac cgcctacaga aaatccagac ggagaacaga
    27781 aaccggaaat agaacctgga gaagaacccg atacagaaac tcaaccagag ccagacaata
    27841 aatcaaaacc tgaaataacc cctggagaaa aaccagatgt aactccagaa ccagatacgg
    27901 attctggaaa ccaaacagta ccggaaacaa atccagacac agataatgaa actgaaaatc
    27961 cagaaaaacc agaggtagat ccagaagaaa aaccagatgt gactccagaa ccagatacgg
    28021 acgcaagaga ccaaggaata ccagaaaaaa taaacaaaaa aacaattcaa gaggatggaa
    28081 aaaaagagag taaaaaatca aatctagcaa ttctaaaaat aaatgaagag cagctgaata
    28141 aaaaatcacg aatatttgat agcgcccaat cagctgaaac cttaaaaagt agcaaggata
    28201 caacctttgc tgtcctgaaa cgaaaaataa acagctacca aaatccggag agtcacaaaa
    28261 caaagttata ctgtggagcg gaatcattct attaagtata gcaacaatgc tttctgcaaa
    28321 aagatttaaa caaaacagat ctctctaaca tttgaataat gtctgtgaac gcatttttca
    28381 aataaaaaga aatatccaga aaatgacccc aaaataccag attgaaatct gatatttggg
    28441 gtcgttcata ctttatcttt cttcatattt attttaaggg atatctgttt cttttggaag
    28501 atggttcggg atctttttca ctttcaatac atctaaaaag aaggtaaaga tcggaatgcc
    28561 gacaatcaag ccccaaacgc caaagaaccg ttcgctaaca agtaagacca caaatgtata
    28621 aaaaatcggc agttctgttt tgctggacat caacttcgga ttcagcacat aagactcaat
    28681 tagatggaca atcaaaatcg taatcaggat ataaacaaca tctcggatgc ctccttggga
    28741 ataagctatg aaagatagag gaatacaaga aatgataaca cctgctactg ggatcagact
    28801 taaaatgaaa atcatgatcg ctaaactcag cagctgatga aagccaaata cagccaaggc
    28861 gattgtagtg agcaacgtat ttaaaagagc gacaacaaat tgagcttcta ataccaaacc
    28921 aaatgtattg acaaacttat cggcaaaata gtaaatatct tgaaagaacc aagaaaattc
    28981 actttttaaa aacaatcttg agaatacaat tgtttttttc ttttcaatca taaaaaagaa
    29041 acttaagatg aatgacatag caaacgccac tgccattttt cctatatcct gtaaatagcc
    29101 aagcgcaatc gaagcgccat tttgaagctg ttcaaaaaaa tcgtttcgct caatataagt
    29161 atggatatat tgtaaaacag gatctgcatc ttttggctga tgctcgtaaa aattaataac
    29221 ggactggatc atgtcaaatg tttgatgaat aagcatcggg atatactttg tgaatgccag
    29281 atagataagg taaacgatca gtgtataaat catgatagct aaaacacctg tagggattcg
    29341 gacaaatcgc tgaatgattt gaacaagatg aatcatcaaa taagtaaaga taaatgtgag
    29401 caaaatggtt gtaatcatac ttctaactaa gaaaagaaca aaaacgacaa ataataaaac
    29461 agtgagacgg cgtatttttt cattcgccaa aaaacgctga taaacggtca aatcgacaac
    29521 ctccaaataa caaaaaataa acacgttatt cattatatga tggaatatga aaataagaaa
    29581 gagcaaaaag aaaaaccgct aaatgacgtt atcttatgag ttggttttcc tcataaaatc
    29641 gttcatctag cagtttcagc cctttttctg gtcttgatga aaattatcta gcatttttcg
    29701 ggtcagatgt tgcagtgtat ccaattcttc ctgttcaagt ggaagataag acttgatctt
    29761 ttgtggaata ccttctgctt gtttttgcaa cgcagcacct tgagcagtca gcgtaacatt
    29821 tacgattctt tcatcacccg tttcacgctg acgattaata taattttttt ctgctaactt
    29881 ttttagtaag ggagtcagcg taccagaatc cagacacaaa tcatttccta gctctttcac
    29941 tgaagtcttt ttcttttccc ataaaactaa catcacaaga tactgggtat aggtaagatt
    30001 cagcggcttg aggaggggag tgtagtgtct aatgatttct ttagaggcgg catataaagg
    30061 aaaacatact tgattaacta aatatagatc ttccattttt ccacctccgc tgttttacta
    30121 tagtgtatca tagcggattt acacaaaaaa gtctattcac taactgcttt cagagtaact
    30181 tctatatttc cagctgtagc ttttgaatac ggacacactt tatgcgctgc ctctaataat
    30241 tcttgcgttt cttccagaga aagaccttcg atagatcctt caatctctac acctaagaca
    30301 aagctcgttt cactttctga ataaagggaa acttgtgcac tgacagtaga agccccagag
    30361 atattttttt gctgtttgat caattctaat gcactgttaa aacatgcact gaaaccagct
    30421 gcaaacaatt gttcagggtt ggttgcattt tcgacgcgtt taccaggagc tgcaatattc
    30481 aattcgaatg tacgatcggg attatgcact tctccagagc gtccgcctgt gttgatcatt
    30541 ttcgtagaat acattttttt cataaaatta ccacctttaa ttatatttta tacaattgaa
    30601 ttttacacaa tataattgtg gaaagcaaag aaatggattg gcataagata aataagagaa
    30661 agcacatgaa acagacgcgc tttctcgaat agcaaacata tacaaaaaat atcagagaca
    30721 aaaaataatc tcatcataaa cattacatat atttctcaaa aagttgtttt ctttcttcaa
    30781 aaattacttc acgatggacc tgatgaagca tccataaata accaaaagga tctctgaaca
    30841 ttgagttagt cacaccaaag tccatcattt ctgtcatttc ttgaatctct acacagccat
    30901 ttcgtaatgc tttattatgt gtgtcctcga tatcagagac gatgatattg aaccagatcg
    30961 tatttggatg ttcagcagta ggagcagtca aaccaagaga cgtgttttca tccagcatat
    31021 ggaatctcgt accatacaag gtaaaaatga cttcattatt gcctctaggg taatctgttg
    31081 cctcaatcag ttgtatttca tcaaagattg tttgatacag ttctagtgct ttcaaactat
    31141 catttaatac aaaatcgatt tccgttcctt tatgcataaa gatctcctct ccttttcagt
    31201 cgaacagtta ctagattctt ttattatacc agctaaagaa aacgcattca aaaaaatagc
    31261 ggatttactt accaactcat tcgattggat gataagtaaa tccgcgtccc attacttctg
    31321 ctacgctgct gacaatgaca aaggcacttg gatcgatttc ttctatcaat ggtttcattc
    31381 gggaaaattc atgatcagtc acaacgatca ataaaacctc ttttgacttt cgattatacc
    31441 cgccttttgc ctctagcagg gtaaggccac gtccaatctc agcacttaag cgaaggcgga
    31501 tgtcttccaa ataatgttca ctcatgacca taacagattt ctttcgattg atacctgttt
    31561 cgatataagt catgacaatt gaagtgattg caatagataa aatagcataa agaaactctt
    31621 ctattccaaa aataaacaga ttaagagaaa caacgaatgc gtctgtaaaa aataagccaa
    31681 tcgaagtatt caagtggaaa tattttttaa aaatgagggg agggatcgtc gtaccgccgc
    31741 tagaagagtt gtttttatat aaaatcgcaa ctcctaaagc aaaaatcgca cttccaaaaa
    31801 tgacagacaa caaccgatct gaagtaatca ttgtttctgg aactattgca atgattaaag
    31861 gaaaaaccaa gcttccgaaa agtactttaa aaaagggttt tttacctaaa aatagaagag
    31921 ctagtaccaa cataacgata ttcaacacta aaataactat agctcggtca aagcctaaag
    31981 cagcttccag taatattcct agaccgctga cacctccagc agcaatatga tgaggcccta
    32041 aaaacatatt gatagcgact cctaatatga agagggcaaa aataatctta atgtatgtcc
    32101 atataaattg tctcgtttct ttcatttatt cacttcctta attcattaat aatactctta
    32161 tcttattata acttactttt caccttaagt taactttaat taataaaaaa attaggctag
    32221 tctaaattat tatgtttcat tcttctagaa tatacttcta cgccaaaaac tgttaaaaaa
    32281 cttttacttt agaaaattgg agaaattgat cgcttaagat aaattcagga cactaaatag
    32341 tacaggaagt agactaaaac ttttaaaagt tttaagtcta tctatttggc aagtttaaaa
    32401 aataaaaatt cggtatcaaa aaactgttac cgataacatt tttgaaagcg ttgtcttttg
    32461 cttctctatc acttatagtg atgacgtcat attaaaacac aaggaggaaa tcaatggata
    32521 cagctgcaat ttatcatcga ccggaaagtg aatatgcatt tctatatacg aatacaagat
    32581 ttcgtattcg gcttcgtact aggaaaaatg atatcaaaaa agtatatgtg ctatgtggag
    32641 atccatatac cataacaact gaaaaatggt atcaaaaaca acgaccaatg aaaaaatgtc
    32701 taagtaccaa tgttcatgat tattgggaaa ttgaagtgac gagcgaaaca agtcgcttac
    32761 agtatgcgtt ccatgttgtt ggggaagata acacggactg tttttacggt gatcaaggaa
    32821 tttttcctta tcgagaaaat gtgattactg aaccaaattt ttattttcga ataccttatt
    32881 ttcatcaaat cgatcgattc actattccag aatgggtaaa agaaacagta tggtatcaaa
    32941 tttttcccga aagatttgca aacggggaca aagcaaatga ccccgaacac actttacctt
    33001 ggggaagtaa agatccagac cgagaagact ttttcggggg agatttgcaa ggtgtgatgg
    33061 atcatttgga ttacttgacg gaccttggca tcaatggcat ctatttttgt cccatattta
    33121 aagccacatc caatcataaa tacgatacga ttgattatta tgaggtggat cccgcttttg
    33181 gcgataaaaa acttttaaaa aacttgattg atgaagcaca taaaagaggg atacgtatca
    33241 tgttcgatgc tgtattcaat catatgggtg tccattcacc acagtggcaa gacgttctca
    33301 aaaacggaga aaaatcgatc tataaagact ggttccacat ccgttttttc ccagtagatt
    33361 cttatcagat gactgactta ccagaaactg ctgaaaatat cccatatgat acttttgctt
    33421 ttactccatt catgccgaaa ctaaatacag caaatccaga agtgcaaaaa tatttgctgg
    33481 atatcgcaac ttattggata aaagaattcg atattgatgg atggcgtctt gatgttgcta
    33541 atgaagtcga tcatcatttt tggaaaaaat tcagagaagc agttacagaa atcaaaccag
    33601 acatctatat ccttggagag atttggcatt cctctcaagc ttggctgcaa ggggatgaat
    33661 tccatgcagt gatgaattat gcatttacag attcgattaa agattatttt gcaaaaaaga
    33721 aaatcacagc ttcacaaatg gtcagtggaa tgaatcatca gcaaatgctt tacagagatc
    33781 aggtgaacga aggaacattc aacttattag attctcatga tactgccagg atcttgaccc
    33841 tttgccaagg taacaaagaa ttgatgaagt ctgtgatggc ctttatgttt cttcaaaaag
    33901 ggtctccttg tatctattac gggacagaaa tagggatgac tggtgaagat gacccggatt
    33961 gccgcaaatg tatgatatgg gagaaggagg aacaagatct agagctattc ggttttataa
    34021 aagaacttgt ttctctaagg aaacagttgt ctaagattat ttctgaaggt tctactcagt
    34081 ggctaatagt aaatgatcgt gaagataagc tctatttcac acggaaacta gagggacaga
    34141 tcatctatgt ttactttaat cagtcaaaag aaccttgggt agtagaacag gaaaatgagg
    34201 tgattcttag tcaaaattgt cagttgttag aagacgggaa agcagagata cgacaatacg
    34261 gatttttgat catgtgttga aaactgttac cggtaacaac aagaaagcgt ttgcaaatga
    34321 agatttgtgc ccattataat aagggtgtaa cagcacgttg aatacaaagg aggaaatgaa
    34381 gatgaaaaaa agaagtgtga aacagttggg tttagggtta atctctttag gaatcttagg
    34441 cggattagcg gcttgtggca atggtaactc ttcagcagaa ggggaagccg atgaaggaaa
    34501 aacgttaaca gtttccgttg attctggata taaagattat ataaacgaaa taaaaaacac
    34561 tttcgaaaaa gaaaacgatg tgaaaatcaa gctagtagaa aaggacatgt tcgatcagtt
    34621 ggaatcatta gcgttagatg ggcctgctgg aaagggacct gacgttatga tggcagcata
    34681 tgaccggatc ggtgcgttag gacagcaagg acatctggcg gaagtaaaac tgggaaatga
    34741 agcagcttac gatgaaacag acaaagccca agtgacttat gatggaaaaa tctacggaga
    34801 acctgcagta atcgagaccc ttgttttgta ctataacaaa gatttagtcg atacagcacc
    34861 atcaacgttt aaagatttgg agaatctatc caaagacagt cgttttgctt ttgagtcaga
    34921 agcaggaaaa aatacaggat ttttagctaa atggactgac ttttattatt cctatggttt
    34981 aattgcaggt tacggtggct atgtatttgg tgatgatggc acaaatcctt cagatatcgg
    35041 tttgaataat gcaggtgctg tagaaggaat ctcttatgca acagattggt tcaaaaatgt
    35101 ttggccaaag ggaatgcaag acataaaaag tgcaggagac ttcgccagcc agcaattcat
    35161 gtccaataaa actgcagcaa tcattgacgg tccatggcaa gcgcaaacat acaaagaaaa
    35221 caatatcaat tatggtgtag ccaaaattcc tactctaaat aatggacagc cttatcaacc
    35281 gtttggcgga ggaaaaggat gggtagtcag caattatgct aaaaacaaag acctctctca
    35341 aaaatggctt gattatgtaa cgaatcagga aaatcaagaa aaattctttg aaatggtaaa
    35401 tgaaatccct gcaaaccaac aagctaggga aacagcaaag gctaaaaacg atgaattaac
    35461 aacagctgtg attgaacaat atgaaacagc tcaagcgatg ccaaacatcc cagaaatggg
    35521 cgaagtttgg acaggtgcag aaaacttgat gttcgatgca gcatcaggtt caaaaacacc
    35581 aaaagaatcc gcagatgaag cagtaaaaac aatcagtgaa gccatagaac aaaaatactg
    35641 attaaaatga taaccgtcca tttttataaa aaatggaaca ggtgcttcag ataaaagtgg
    35701 caagagcgag atggaaaatt atcttgctct tgttgccatt atcttaaaag cagtttgcgg
    35761 tttagcaagt ttagaggggg cattcagatg cagcaagata aaacaagcaa tatcaacgaa
    35821 aaaaaaatca cgctttgttc acttattcca gggatggggc agtttgtaaa tggccaagct
    35881 tttaaaggta tcgttttttt cgccatattc gtcctattca ttggtcagat gcttctgggc
    35941 ggatttcatg cattggtagg attggttact ttgggaagta taccaatgga agatcattca
    36001 ttatttattt tgattgaagg cactttgcaa ttcatcatta caatcatttt cttgattttt
    36061 tacggtttga atcttttaga cgcttatcga gtagcgaaga tgcgtcaaga gggcaaaaaa
    36121 atcaatcgga cgatgaagga agtcatacat aatataggtg atgaaggttt tccttatcta
    36181 ttgacacttc ccgcttatct tctcatgatt tttacgatca ttttcccagt cctagttaca
    36241 ttgttcactg catttactaa ttatgacttc aatcatattc caccagccag tttaattgat
    36301 tgggtagggg ctaaaaactt ttttagtatc ttctttttaa gctcatatcg taatactttt
    36361 tttgcagtct tttcgtggac cgtgatttgg actttatgtg caacaacttt gcaaatcacc
    36421 cttggtattc tcacagcggt tgttgctcac cagaaattta ttaaattcaa acgtattttc
    36481 ggtatcattt tcttattacc atgggcggtc ccagctttta ttactatcat gagtttttcg
    36541 aatatattca acgatagcgt aggtgccatc aatactcaag tgattccttt cataaaccag
    36601 cttccttttg tagatatcag tgcaatctct tggaaaacgg acccgaactg gacgaaagta
    36661 gcaattatct tagtgcaggg ctggttagga tttccctata tctatgtgat ggttacagga
    36721 atcttacaat cgatttcaga agacttatat gaagcagcaa aaatcgacgg agcaaatgca
    36781 tggcagcgct tttcaaacat cacgcttcct gctgtcttcc tagtggcagc gccaacattt
    36841 attacacagt atacggggaa tttcaataat ttctccatga tctatctgtt taacaatggc
    36901 ggtcccggaa gcgtaggcgg aggagctggt tctactgata ttcttatttc ctggatctat
    36961 aaacttacga ctggaacttc tccgcagtat tctatggctt cagcaatcac gttgattatc
    37021 tccgtcatcg ttatctgtgt gtcattgctg gtatttaaaa agacaaatgc gttcaatatg
    37081 ggggattaac aatgaaaaaa tttcattcga gtgctcgatt ccaaaaacga ctttctcact
    37141 tttttactta cttttttctt ttaatactgt cagtcattat catctatccg cttttgatca
    37201 cagcaagtac tgccttcaag tcaggaaata ttgcagcttt tagtttagat ctttcaagtg
    37261 actggacttt aggaaacttt actcggctat tcagtgaaac actttacggg acttggtata
    37321 aaaatacatt gatcatttct gtcctaacaa tgatcgtaca agtgactgta gtaactttgg
    37381 cgggctatac ttatagtcgc tatcgtttta tcggccgcaa atcaagtttg atgttctttt
    37441 tagtgattca gatggtaccg actatggcag cattgactgc cttttatgtc atggctttct
    37501 tattagaagc gctcgataag tactggttct tgacaatgat ctatataggc ggtggaattc
    37561 caatgaatac ttggctgatg aaaggatact ttgacactgt accaatagat ttagatgaat
    37621 ctgctaaact tgatggggca gggcatttca ggattttttt ccagattgta ttaccgcttg
    37681 tcaaaccgat gctcgccgtt caagcattat gggcttttat gggacctttt ggggatttca
    37741 tgttagctaa gttcctgctt cgaacacctg aaaatcagac tgttgctgtc ggcttacaaa
    37801 catttatttc tgatgtgaca aaccaaagag tctctctttt tgctgctggg gcaattttga
    37861 ttgcactacc gatttgtcta ttattcttct ttttacaaaa gaactttgtt accggcctga
    37921 catctggcgg aacaaaagga taggagcaga gtatcatgca aacatttccg ttaaactatt
    37981 tttccgcatt acgtacgcca actcaagtgt ttgcgggacg caaactatta tcatggccta
    38041 aatttttctt gatatttgta tttttagttt ccttgatggt catgcctgtg actctttttt
    38101 atgccaatca gatccaagcg attccattag aacaatttct atccgttcat tcgctgatcg
    38161 atgaacaagg aacccaaaaa ttttctgaac ttgaattatc tgaaactggg ttacaagcat
    38221 cgcaacaaac gatagctgtg acccctgaaa ttttggtagg tgtgtcttta tctgagaaac
    38281 aacaatccga tcatggaact tttattgatt ttgagaagga acaatgggtg atccaacaaa
    38341 aggataaatc tggtatacgc cgctatacga tgaattatag cccatctttc caaccagatt
    38401 ctgtacgtac gcccgaagat ttccaacgat ttttagaaag agaattttat gccagtaatc
    38461 gtccaacaat cattttatct tatagtttaa gtcttggtct tgtcctattt gtcatgacga
    38521 gtctcatttt attcggggct tcctttttct tgtggatgac aagaaaaagc caactttcat
    38581 ccattcatac attcaaagag agtgcaaatt tgatgctgaa tgttatggga ataggttcca
    38641 tggcagcagc agttgtaggg tttatccact ttgactttat cttgatgcta agtgtccaaa
    38701 caacgataac tgtcttgctg cttttatggg tttttgtcaa aacaagattc aaagataagc
    38761 gtgtcgaatg acaagtttaa gcgatttatg cgacaatgaa tgtgaaaaaa atgaaaaata
    38821 aagtagtttt tgaaaaattt ctgaaaaaat taaacttctt agttgattct tttctttttt
    38881 agtattaacg taatagggta gtttttttaa ggaggaaaat catgttagag aaaactttat
    38941 acaatcaatt attttcacgt tctttctcgc tccctgtcga agtaaattat tgggacggta
    39001 caacgaaaca atacggagat acggacactc ctacccaaat caaaatcaat atccatgaag
    39061 aaatcccaat gaaagagatt acaagtaacg catctttggc actaggagaa gcctatatgg
    39121 atcatcgaat cgagatcgag ggaagtatcc aagcattgat caacgatgct tatcagcaag
    39181 caaacagctt tttacgagga aaagattatc taaaatggct gccaaagaaa gaaaaacata
    39241 cgaaacaaaa aaacaaagaa gatatccatg cgcattatga cttaggtaac gacttttata
    39301 aactttggct agatccaact ttgacttatt cttgtgctta tttcaataca cctgaagata
    39361 cgttggaaca agcacaaatc aataaagtcc atcatatctt agacaaactc tttatcaaag
    39421 aaggcgaaac gttgcttgat atcggttgtg gctggggaac attgatgttc actgctacca
    39481 aagagtataa tgtcaaggca actgggatca cactaagtga agaacaatac gatttgatta
    39541 ccaaaagaat aaaagaagaa catttagaag ataaatgccg tgtattatta atggattacc
    39601 gcgaactaaa aggcgaaacg tttgatcata tcacaagtgt aggtatgttc gaacatgtag
    39661 gttctgaaaa tcttgaaggt tatttcaaag tggtaaaaga tttattgaaa ccaaaaggaa
    39721 cagctttgat ccatggaatc agccgacaac aaggcggtgc gacaaatgct tggatcaata
    39781 aatatatttt ccctggcggt tatgtccctg gtgttactga attagttgat aatattacaa
    39841 aaaacgattt gcaactgatt gatttggaaa gcttacgtcg tgactaccaa ttgacattag
    39901 aacattggac aaagaatttt catcaagtcc aagatcaagt cacgaaagaa atgggtgatt
    39961 ccttctatcg aatgtgggat ctatatttac aagcttgtgc agcttcattt gaatcaagta
    40021 atatcgatgt gattcaatat ttgcttgttc aacctggaaa caatgacatc ccaatgcatc
    40081 gtgcataatt gaggcaaaag tactgtttaa agactattta aaaagaagct aagttttatg
    40141 atgctccaga agtgaacttt tttgtctaac ttctaggatt gtctctaaag cttagtttct
    40201 ttttctttat attttcataa taaaagtttg gttctttaat ttttaaacat gacagaaaat
    40261 tttttcttcg ttatacttgt aatagaatac tattttataa caaaggggat cgaatcaatg
    40321 aaaaaaactg ttatttatac tagtttattt gcagctttgt ttttagctgc ttgtcaatca
    40381 aataccaatc aaggcacatc tgggaatacg tcttccagta gcgaacagcg ctcttcacag
    40441 gtatcggact cctcaacaaa agcaagcaca tcttcgtcta gccagacaaa aagttctagt
    40501 gaagcaagta ctcagtcaga tatcaaagtg tctgcagctc gtgcgatcga attgttccaa
    40561 gataaatacc cagatactgc aattactagt ttggaattag atagtgattg gggatcttac
    40621 ttctacaaga tagaaggtgt cgatgatcaa aaagaatacg aagcaagaat tgatgctcaa
    40681 aatgaaaaaa tggagacaga gaatccagaa actctagatc gagatgaaca aaatggtcaa
    40741 aagaaaaaag aagatggtct ggatgttgat aaattgatca cgattgagga agcagcaaaa
    40801 atcgccgtcg acaaagtagg aagcggtacg gcaacagatt gggatttgga taaagattta
    40861 ggtactactt attgggaagt caaagtaaaa gacggcaatc aaagtacatc agtcaagatc
    40921 aatagccaaa caggcgaagt actgtcaact gaacaagatg attaagaatt tcttcggaaa
    40981 acaagagttg atttttcttt tagtcatgat acactagaca aaataacttg tttgaaggag
    41041 cgatttactt gagtttagaa acgacctttg ccaatcatat ctttgccaat cctttgatga
    41101 atgcatctgg tgtccactgc atgactaccc aggaattaga tgaactggct cattcagaag
    41161 caggagcatt tatcactaag agctgtacga tcaatgaacg aaaaggaaat cccgaaccac
    41221 gctattttga tgttccttta gggagtatca attctatggg acttccaaac ttaggttttt
    41281 cttattatct agagtatgct cttgcgtatg aaaaagtgca ggaaaaccaa aatcaaccgt
    41341 tatttttttc cattgccggg atgagtgtcc aggaaaactt ggaaatgctt gagaaaatag
    41401 aaaaaagtgg gtttaatggg atcactgaat taaatttatc ttgtcccaat gtgcctggaa
    41461 aaccacaact agcttacgat tttgaggcca cttatgaaac actgaaagaa gtattcagca
    41521 tcttttctaa gccgctagga atcaaattgc ctccttattt tgattttgct cattttgatc
    41581 agatggctga tatcttgaat cagtttccat tgacctatgt caatgctatc aacagtgtgg
    41641 gcaatggttt gtatatcgat acagaacaag aagcagtagt catcaaacca aaagaagggt
    41701 tcggcggcat tggcggagag tatatcaagc cgactgcttt ggcgaatgtt cgcgcttttt
    41761 atacacgttt gaaaccagaa atccaaatca ttggtacagg tgggattcgg acaggacaag
    41821 atgcatttga acatctgcta tgtggcgcaa gcatgttaca gattggtacc gagcttcata
    41881 aagaagggcc ggaaattttt tctagaatca taaaagaatt aacgcagatt atgtcagaaa
    41941 aaggatatac gtcaattgac gaatttaaag gcaaattacg caccatttca tagcaaagag
    42001 acgaaggtca tgattgatct tcgtcttttt ttataaaaaa cactcgtctg ttcattcctg
    42061 tgcaagcgta ctcattgtat gttagacgaa cttgtgggaa aataacagag gcaaaaagga
    42121 ggataatatg aatgatgcaa cagaattagc aaaagagctt aaagaaaaag aatttagttt
    42181 tcaagaacta gttacggata tacagaaaaa aataaagaag caaaatcatg aattgaatgc
    42241 gtttgtcgcg cttgaaccat tcgagagtaa ttcccacgag ctattgattc cccagtcaga
    42301 aaaagggcca ttggctggta tcccatttcc tttaaaaatg ctcggtcaag aaaaaaaggg
    42361 atggctagct acttcaggat ctcgtttatt tgaaacacac cgagcttctc atacatctaa
    42421 ttatgtgcgg caagcagaag cgataggtct agttcctttt ggacaaacca atgcgccaga
    42481 gtttggtttc aaaaatatta cagacccagt catctatggc ccagcaagaa acccttggaa
    42541 ccttgaatgt acaccgggcg gatcaagtgg aggagctgct gctgcagttt catcaggaat
    42601 cgttccttta gctggtgcta gtgatggcgg tggttctatt agaatacccg cttcgttttg
    42661 cggattgatt gggctgaaac cttcgcgagg gacgatgcca gttggtccat atgcttggag
    42721 aggatggcaa ggagctgcta ttgatttcgg cttgacagtt tctatgagag acacggaggc
    42781 tttattttat gggatgagaa gtatccattc cggtgcgcca tatcaagtat cgccagttga
    42841 gtggcaaaca catccaagaa aaaaacggtt gaagatagct ttttgtgttg attcaccgat
    42901 ccactcacag attagtgaag aggcagtact tgctgtaaaa aaagctgtag atttccttgg
    42961 aaaagaaggg catgatttga cagagattcc ttacccacta gatggtcgta aactgatcac
    43021 gtcttattat cagatgaatg gcgcagaaac agcagcaatg atccgctcga ttgaaacagg
    43081 catccagcga gctgtcagaa aagaagaact tgaacccatt tcttggactt tgttccaata
    43141 tggtcagaag ctctctgccg ctacttacat cgattctctg catgtatggg atgaagcagc
    43201 aattgtcatg gaaaacctac atcaagaata cgatctattc ctttcaccaa cgactgcatt
    43261 taccgcccct aaaatcagcg aagatttgca aagtgatgcg ataagagcac agatggaaca
    43321 catcagtgat tgttctgaag tagaaggttt agacgttgtt tcttccatgt ttgaggagag
    43381 cttgaagatc acaccttata cacagttggc taatctgact ggacaaccag caatcagctt
    43441 gcctactcat ttgacaaaag aaaacatgcc tctaggaatc caatttatgg ctgccagagg
    43501 aagagaagat ttgctatttc aaatcggcta tctatttgaa gcaaatcatc agttccattt
    43561 accaaatcca tggaaatagc agaaacaagg agaagaagaa tgaagaaaaa aagattgttt
    43621 gctctaataa ctagtgcctt ttttatttta agcgcttgcg gtacagctgc cgctaatcag
    43681 gaagataaca gctggagtaa tgtagaaaaa aagaaagaat tagtagtagc gacttctggc
    43741 acactgtttc ccgcatctta ttataatgat caaaatgaat tagttggtta tgatgtagat
    43801 ttggcaaaag aggtggctaa acggttaaat gtcaaaatct cctttaaaga atacaacgtg
    43861 gatggacaaa tcacttctgt ttcaaaaaga gaatcagatt ttgctgcgaa cgattttggt
    43921 atcacaaaag aaagagaaaa aaaattctcc ctttcagaga ctttcaaata ttcatttgac
    43981 agtatgatcg tacgaaaaaa agatgattca ggaatccact cacttgaaga tcttaaaggg
    44041 aaaaaagcag ctggcgaacc caatactagc tatatgcgtt tagcggaaaa acacggagct
    44101 aaattagtca catatgacaa tgccacgaat gatcagtatt taagcgatgt agcaaatggt
    44161 cgaacagatg ttattctaaa tgattattat ctgcagaaaa tggccgtatc tgctttacca
    44221 gatatccctg taaaaatact ggagaacgtt tatttcaatg aaaacgaaag tggtttttta
    44281 ttgaaagaag gaaatgatgc tctgaaaaag aaaatcgatg gcgtattaaa agagatgaaa
    44341 gaagatggga cgacaaagaa aatatctgaa aaatattttc aaactgatgt ttcagtcaaa
    44401 ccgaaagaaa cgatggctga gtcggtgagt gagtgatgta ctttcctcta attgattttt
    44461 ccctcatgtg gacttcactt cctttcgtac tgcaaggctt ggtatacaca ttagggattg
    44521 gcttcgtgtc gtttctttta ggaaatctgg tagggttgct tttgacagtt ttaggtcttt
    44581 tggattggct gcctctgaat gtatttattc gtttttattt atcgttcttt agaggcattc
    44641 ccgcactagt cctgctgttt ctgttatatt ttgggttacc ttatcaatta agtgccttaa
    44701 cggcttcagt catctgcttt acgattacca gcagtgcatt tattggtgaa atctatcgag
    44761 gatcactagc aggcgtttct tcaggtcagt gggacgctgg tttagcttct ggtttttctt
    44821 tctttggggt attgaaatat atcgttttgc cgcaagcttt tcgaatctca gtaccggcac
    44881 tcagtaacgt agctatggat ctagtgaaag gtacttcgtt agcagcaaca atcacagtgc
    44941 cagaaatatt tcaaaaagca aagatcatag gcggtcggac attcgattat ttttctctct
    45001 atattttagt tgcactgatt tattgggtca tttgtatcgg gatcgaaaaa gtgcaacagt
    45061 atttagaaaa acgttctatg catcagtcaa tctgaaaaat agaaatggtg ctagcgtggc
    45121 tggcaccatt tctatttttt gtttcttgta tattctgcct agtattttaa aaccatttca
    45181 tgtaaaatgt aaatcgaaaa tatgttcgga ggtagaaaac atgaagaccc cgctaaccat
    45241 cgaaatagaa tcctcgttat accaatattg catcgagcaa ggcggcttgg ttgtagaaga
    45301 agtcacgatg cctgatgacc aaggaattgt agatacgctt agctgcttga caaaatttga
    45361 tggaagccga gaatggcgat gctatgagct gaaagtgaca aaagcagatt ttcgctctac
    45421 agccaaactg tcatttgttg gacattataa ttattttgtt ttaccaagta aactatatga
    45481 agaagtaaaa actgaaatcc ctacaggaat aggtgtttta gtgtatcgtc cgtacacggt
    45541 tgaagaggag atgcctgcct ctggtacatt tctgattgcg aaaaaagcag caaagaaaga
    45601 gcttgctgta ccggaagaag ctttgacaaa ccgcttcatg gcttctttat tcagggaagt
    45661 aagaaaagca aagcaaatgg agcgaggcgt aaaatatttt ccaacagatc aattgtacaa
    45721 agaattgaaa agacgttcag aaaatcaaga tccattttct aacgaacgat attatgaacg
    45781 gtttttagag gaaatagcta gtcaggcaat caatgagttg caagaagaag ttatcgcgct
    45841 tcagcatgat tatgaatatt tgaggcaaca acagcagatc cgtcggttgc ctacagagcc
    45901 attggagtga aaaatatgta ctatccttat ttaagaggaa aacaatttga cctattagca
    45961 ttaaaagaag cactgtcgcg aggattgctc tccaataaaa tccaaccagt gatcgaacca
    46021 gtacgtgatt ccgctacgct taaaaatgtg atcgaattat ttcaaaaaaa gcaccatcga
    46081 atcgctgtga tccaaaatcc ccaagtagga caattcaaat tattcgatca gcatgtgcac
    46141 agttggacag tcaaagaaaa tagctctgtc gtccctgcac agatcttaac gccagaaaat
    46201 ctcagtgaag tactggattc acctccagcg tttcttgttt tcgatggaca gcactaccca
    46261 aaagatacag aggtgtggaa acagttggca ggagttgatt cgaaattttt gataccagat
    46321 actagtcgct ttcgcatctg gctgcccgaa aacaaaattg tgatccgtga ctcttttcaa
    46381 acgagaaagc atgttgaaag ttatgcagat aaaacagatg acttttttag tgatgactat
    46441 ctttttttcc acgcagacgg atatattgga ttttctgatt tcacgatcga aggtagccgt
    46501 tatttcgata aagggttccc tagccgagcg cttgctatcc atctaacgta tattgatgct
    46561 tacggcaata tacgcgtgaa acattttgtc tctgacagta acgactctgc taaagaccaa
    46621 gcattgaagt ttcttgaagc aggagataaa atgaaaaaat ggatcatgcg tcaccatcat
    46681 caactcttga ttacttcagg aatgattgaa ttattggctc tttaccagca acaaaaattc
    46741 cctggactgg gagtgttgaa aaagtggtca cttatgcatc atttggaact tattagtcac
    46801 ctgctagatc atccaaaaga ttggcttaaa tattattcaa aagttcagga actatatgaa
    46861 gtgatccaat gattagataa tgtaaatgaa aaaggaatag aaaagaggaa actggatgta
    46921 tattcggcaa acaaggaaag aagaagtaga agaagtggta gaaatcatta atgccgcagc
    46981 ggatttcatt gcttcgcaag gctctcctca atggcaaaac gggcagaaac caacaaaaga
    47041 aacgattttg aacgatattg aaagaaaaga aagctatgta ttaatagtgg aagatcaaat
    47101 tgctggaaca gctgcattag tctcaagcgt ggatcccgtt tacacaaaaa tagatggtag
    47161 ttggaaagaa tcaactggtc cttatttatc gattcatcgt gtcgctttag cagaaagtac
    47221 tcgaggaaaa aaactagcaa aaccgttctt agaacatttg ttgacgattg cagccagcaa
    47281 aaatatccaa gatatccgta tcgatactta tccagctaat gaaccgatgg aaaagaccat
    47341 ctattctgct ggattcactt atcaagggat gattgaattt ccttttgaac acggcgaaag
    47401 aaaagcatat caaataatca tcaattgact ataaaatcat ctgagcaaag ataaagacat
    47461 taacactact gaaaaaaaca atcatatgat agccaagagg ttgtaacaga agtggccagc
    47521 ttcaaaaaac aagaaagaat ttccgaaaat gcttctcaaa tttttgtgga attttagcgt
    47581 attttacgaa atagctgctt tctgaaactc ccttcatcga gaggtcgtga catagctttt
    47641 gtcacgacct ctcggcttat cctacgagta ttattcgata catttatatc aataatcctt
    47701 tttttatttt atctgcagct tcttggaacg ctccatcgtt tgacacgact aaaagtgagg
    47761 ggatagcttt caaacgtttc aactgtgcta aatcttctac ataaaccgcc agtctggctg
    47821 aaatctgttc agatgaatct ccacgttttt tcaggcgcga ttgcaatgtt tctttagaga
    47881 tcgtaataaa gataggacgg actaccgtcc cgaaatactc atataaatgc caaaaaccat
    47941 ttgctgtcaa aacggcgtaa caatttccta ctgtcagttt ctttttaatt tctgctttac
    48001 tggaaccata tttatgttga gcatattggt cccactctgc aaactcattt cccttgatca
    48061 tctgattgaa tgtattgtca gaaacaaaat aataatcctc ttgatctttt tccccaggtc
    48121 ttggtgatcg attcgtgtaa gtaatgatct tttgttccgg ctgaaaaata gcttctgcga
    48181 gtgaagtttt tccagaacca gaaggaccga taaatacata gcaataattc attgtcattt
    48241 ctcctttgat gattgaacaa gtgaaaaagc tttggaaaat atttccaaag cttttaaaag
    48301 taactttgct tttttgtata ttataccagc aagccagtca caatagcact taaaaagctg
    48361 actaaagtcg ctgttagcaa aattttcaat ccgtgttttg cgacaagatt tcctttttct
    48421 tcatttaatc ccttcatcgc accagaaatg atcccgatag aagagaagtt ggcaaatgaa
    48481 acgaggaaga cagatataat tgctgttgta cgctcagtaa aatggaaatt accatttgct
    48541 aattctgtca tagcaacaaa ttcatttgta accagtttag tcgccatgat actaccggca
    48601 tccactgctt ctttccatgg gattccgctg atgaaggcaa gaggggcaaa gatgtaacct
    48661 aggatctctt ggaaagtgat gccgaatatc ccgtggaaga tagcattgat catggcaatc
    48721 aaagcaacaa aaccgataag catagcagca accgtgatcg ccacatggaa accatcaaga
    48781 atgtattctc ccaacatttg gaaaaatgtc tgttctttgt tttcttcaat gatcagctca
    48841 tcttcttttt cttctaatac atagggattt acaattgaag ccagaatata accgccaaat
    48901 aagttcaaaa caagggcagt gatcacatat ttagaatcga tcagtgccat ataagatccg
    48961 acgatcgaca tagaaactgt agacatagcc gatacactca tggtgaacaa acgtttctca
    49021 gataggaagg gtaattcctt ttttatggaa ataaaaactt ccgattgtcc taaaatagca
    49081 gaagctactc cattataaga ttccaatttg cccatcccgt tgattttact taatcctaga
    49141 ccaacagctt tcataaataa tggaagaatc ttgatatagc gtagaatacc gataatagca
    49201 gaaataaata caattggcat caatacggaa agaaagaaag gtgcacttcc tttatttgct
    49261 acaccggcaa aaacaaagtc aacaccttgc ccagcaaaag caagaagatg atcaaacatt
    49321 tttgccaata cagaaacaac cgctgtacca aaagttgtac gtaataatac gaaaccaaga
    49381 acaagctgta aaacgaacat cacaattagt ggtttcaatt tgattgcttt tttgttactg
    49441 ctgatcaaaa aagcaagccc taatgtgaca aataaaccaa taatacctaa taaataactc
    49501 aaataatcac tcctattcca aattccgttt tattttacct gttttgcaaa taaaatacta
    49561 atctttagtg aaattttcat tactttttta gagtgaaacg cattcatttt ccattagaaa
    49621 ataaagcgaa caaaataaaa ggtttaaaaa gtgtaacgtc ttagtaaagt ctataaaaaa
    49681 acgaaactat cgattaagct aatagtttcg ttttcaacat ttataagtaa actttttcta
    49741 ttggtaatat agattttcgg aagggtagac aggtgcgcag gtaatatcga ttcctagttt
    49801 tttcaatgtt tgttcatcat ttttattgac catcacagta gagtgggctt gtgctccctc
    49861 aagttcagct agtttatcat aagcaacttg agcgattgga ttcgttactg cactgattga
    49921 taaagcgatc aagacttcat tgatgcttag tgcatccaaa gaggaatgaa gtgtctttga
    49981 tttcaatcct tgtattgttt caagaactaa tggtgacaat aaaggaatgt catctgcgat
    50041 atgggctaaa tgttttactg catttaagat aactgccgct gacgcatcca tcaatgaact
    50101 tgtacgcccg gtaatgatga cttcatctgg taattcgatt gcaatcacag cagaaggatc
    50161 ttttgtagga ctttgcgctt gaatcttatc tgcatagtca tgggcaggga ctaccacgtt
    50221 tcggtcttca ggacgtaaac cgacttcttc cataatcagt ttgattcgat taactgcttc
    50281 ttcgtcgatc aatccttttt tgaagtcgca ttctgtttgg aaataacgac ggatgatttc
    50341 ttgtttagag gcttcttgga caacggtatc atccacgatc ccaaatccta cacgattcac
    50401 acccatgtca gtaggagatt gataaacaga ttctttacca gtaatttttt caataatacg
    50461 tttgattaca ggaaatgtct caacatcccg attatagttc acagctactt tattgtaggc
    50521 atcaaagtgg aaggaatcga tcatattgac atcttttaga tcgactgtgg ctgcttcata
    50581 agcaatgttc agcggatgtt tcaacggaac attccatact gggaaagttt cgaacttgga
    50641 gtaaccagct gcatgtcctt tttggctttc atgatacagt tggttcaagg aagtagcgag
    50701 ctttccactt cctgggccag gagctgtcac tacaacgatt ggtttagttg tttcaatata
    50761 ctcatttttt ccaaaaccat cttctgagac gattttttct acgttgactg gatagtcttc
    50821 gatttctgag tgtttataga ccttgatgtt tcgtctttct agtttattga taaagacttt
    50881 cgtggcaggc tgtccactat agcgagtgat aacgacactg ttgattaata agccataacc
    50941 acgtaattca tcgattaacc gcaagatatc catatcataa gtaatcccat aatcaccacg
    51001 gatcttatta cgttcaatat ctccagcata gacgcaaatc agtatttcag cttgatcttt
    51061 caatttttgc agcaatttga ttttcgcatc ttcttcaaat cctggaagta cacgtttcgc
    51121 atgcttgtcg tcaactagct tcccaccaaa ttctaaatat aatttgtcat agtggttcac
    51181 tcgttccaaa atatacgctg actgctcttc aatatatttt tctgaatcaa aaccaatttt
    51241 tttcactagg ttccctcctt aagtactaaa gcattttttc atttttttga ggattcgtca
    51301 actggaagag cgaaattcct caaaaaaaca aagaaaatgg tttaaaccaa cttgtctaga
    51361 tagttattca ttggtatgat agtttttgat aaggaaggtg ctacatgaaa aaaagacaga
    51421 aaaagaaaaa cgcatataaa cactatatcc gttcgatttt taccggttat gaaagaatgc
    51481 ttgaagatcc cgaattggaa caattgacgt ttacttatct aaacgaagaa acgcagctaa
    51541 ctcgtgatga gcatcagcgc attcatttca caacaagaga tctgcctagt aaataaaaaa
    51601 taagataaag gtcatcggat tcgattctgt cctatcagat ggaaagacta gttgatcgaa
    51661 aaaaaggctg taccaaattt tggtgcagcc tgttttatgg agcattgccc gctctttttt
    51721 ataacaattt tttacagaaa atggtactat tgaagtatta ataagtgaaa aacagatgag
    51781 taagaggagg caagtagtat gaaagagcca gatacaaatg caaattatga gccagacgaa
    51841 ctagaggaga cgaatgcgga tcaggataca ggacaagaat cgtcttcttt ttacgatatc
    51901 aaagaaaaag caagcatgaa tctttcccaa ttattagaag acttacaggc ttttgaaaaa
    51961 gctgtccgcg aagaagatat ccctgagatc taccgtttat acaatggccg attgaatgaa
    52021 gaattaaaaa gaagctcaaa tgaaaatcac gagatcgatc aattgctgat gcgtaagatc
    52081 catgatcgtt ttttacagga atttcctttt atggaacaag tagaaaacat ctcgccaacg
    52141 atgagctatt acaaaatcgg cacttattac catgatcggc ccacggtcgg tattgatgca
    52201 agtctgccag aaatttttgt tttgcctaag atcgatgaag agtgggagaa atattcccaa
    52261 ccggagtcaa cggactatga caaaaaaatc aatgagctag acgcaaaggt gatcactgct
    52321 cagacagaaa ttgaacgttt agatgaacaa ataaaagaaa tcaatcgaca aatgactgat
    52381 ttgaacgaca ataaaggatt cttgaatcgt aaaaaaatcg aggaagaaat tcaagaatta
    52441 gagaagaaga agcaagtact atctaatgaa aaattaggct ggcttcctta tattgagacg
    52501 cctgaaacaa tccagcagca aaaagaagca ttgaagcaag aagcaagagc agaccagctt
    52561 cgtgcagcca tcgtagaaaa agaacaaagg caaatcagac gctatttcgg tagcaaagag
    52621 ggattcggtc aagcaatcca tgaattcttg atgaattatc ttggcaatga gaatcctaat
    52681 aatcaatcta atgaaggagg ttccgagtat gaatgaacac gatgaagcag aatggacaaa
    52741 aaatgaagaa caaacagaag atcatgaaga aacgaacact tcattggaag aacaaacaag
    52801 taatcctctg gatgacacag aagaaatccc tatgctagag gaaacaaaaa tacacgaaaa
    52861 agaaaaagta actccgacga atacgaatac tgcagaaaac acccatgctt acgaaataac
    52921 tgaaaatccc gggtatttag aaggaaaaga gcaagatcat caagatatcg tcagtttgat
    52981 tgaaacagcg gaagcagaat tagcggattt acgtatgaga aaagatttga tcgaatcaga
    53041 ttttccacaa ttattagaat catatgatcg ttttatattg aatgaagaac caatctccac
    53101 gatcggaaag acaagcctga tcgaatactt cacgtatgaa ttactgaaac cgttgaatga
    53161 tattggtctg gaacggtcaa attcagctta tgacacatgg aaaacaagac atttttcgat
    53221 caattatacg ttgaatggac aaaacgaatt agtcttttcc tttgtattac cgaccatcga
    53281 tcaacgatac caagtggaat cgatgagtct cctaaacgta tcaccagaga caatggaaat
    53341 caatgtccaa gatgaccggg tgctttcttt gatccgacac tggtcagtgg atcgagtatt
    53401 ctctgcaggg cagatcacga tattcaatca caaattgaat caattattag cccatgcaag
    53461 aacacttggt tttaccgtta atcagacatt actagacaat acgaagccac ttcgtttgaa
    53521 tcttcagagc gaatttgaat tgacgaatga agtattggat gatattttta tcgtggcaat
    53581 gaaaaatcca tcctacgata tggaaaagat caacgaaact acttatcaag tacgattaga
    53641 taaaggacag tctttaacga taacaacaaa cgaaaaagat caaacagtct tgtcgatctc
    53701 atctggagac tacccacgat ctgtcattga tttctttatc aattacgaat tcttggtccc
    53761 attaatggtg agaaagtcct gattggataa gttggattac cagatgtcat tcagataatt
    53821 ctgttccaaa ggacttgcgg cagttatcca gaataattgt cacaagtctt ttctgtatgt
    53881 actaaatgga cgtgaataac ttgctgatga aggagggatt tcattaggaa tgtccacaaa
    53941 taaaatagca cagcttagct tattgtctgc tgcttgtgta gcaggtagga tcgcttttca
    54001 atttattccg aatttccagc caatgactgc tattttttta tttatagtac tttacttaag
    54061 cttaaaagat gcgttaattg tcatgagtct ttctattgcg atcagcagtt tttattttgg
    54121 tgttggacca tgggtcatcg gccaatggat cagctatact gttgttctga gtttgttttc
    54181 aatcgtctgc ttgagaaaaa cagtgcaaag gaatctatgg ttaaagggag tctgtttttt
    54241 tctggcgggc atggtttacg gaataggtat gaccgttttt gatacgctgt tatatcgttt
    54301 gcctcaacct tggatctatt accttcaagg ggtgtcgttt gatttgatgc acagtatagg
    54361 caatgttgtg ttctttcttg tttttctgcc agtcgtcaaa cgtttttaca atcattcagg
    54421 aggaaaaaat gaaaaaaata atttatagtt tgcttgcaat gagtgtcagt atactcattt
    54481 tgacaagttg cagtacaacc tatacacaat cggcttccaa ccaaaaggag gaagtaaaaa
    54541 catcgcaaaa agcaacaatc acgttacaag aaaatggtca aaacttttcg gaaaaagaag
    54601 tgttcttcaa aaaaggcgat gatttactga gtattttgaa acgaaatttt gaagtcaaag
    54661 aagataacgg cttcatcact tcgcttgaag ggcactcaca ggatgagaaa aatcaactct
    54721 attggatgtt tactgtggac gggaaaagtg ctactactgg tgccaaagag atcaaattga
    54781 atgatggaca atcggtcatt ttcaatttgt ctaaactata agtttcataa aaaactgtta
    54841 aagggcgaat attgccataa ttcagtgagt tcagttgatg aaagaaggaa aataacatgg
    54901 aaaattttaa aaaaagcgga atgttttccg taattgctgc tctaggggct aatattctag
    54961 ttgctatcag caaatttgtc ggctatgcaa tcagtggcag tgccgccatg ctaaacgaaa
    55021 gcatccacag tatcgttgat tgcagcaatc agattttgct gttattcggt gataaacaag
    55081 ccaataaagg acagagcgag ttgcatcaat ttggtgaagg acgagcaaaa tactttttca
    55141 gtacaatcgt cgcaatgatg cttttcttcg gcggtggggc acttggggtg atggaagctt
    55201 ttgaaaagtt gacgcatcca tcccatgaag tgggtaatcc gatcattgtt atagcaatac
    55261 tgttatttgg tttggtaatc gaaggaagtt cgttacgcgt ggcaatgaaa gaaatcaaag
    55321 aattgaatac tgaaaaactt tccacgatgc gctttttgcg cgaaagtcga cacagtgaaa
    55381 tactgattat ttttacggaa gacttgtgtg ctgtaatcgg tttggtcttg gctttagcag
    55441 gaacaatatt gacttatgca accgggattg ctgcatttga tgcgatcagc ggattgctga
    55501 tcggtttctt gctgatggga gccgccattt tcctagcaaa agaattttat agtctgatca
    55561 tcggtgaaag cgtgacagca agcgatttat caaaaatcaa gttagctttt gaacgtcctg
    55621 agatcagtcg gctgatcgat ataaaaacag ttcatttaag tccaacggag attttagttg
    55681 cagcaaaagc agatatcgtt ggtaacaaag agaatgaagc ttactcggtc atcaatgata
    55741 ttgaaaaaaa tatgcgtcag tctcttcctg acaagaagat gtatatatac attgaaacag
    55801 ataaatatga cccaaattat tctcgtaaat aaaacgttta ttcatgaatt gaagcaaaaa
    55861 tgagaaaaat ataatcttgt ttttaaataa aacggctaca atttagcgaa tttatgttac
    55921 aatgagtccg ctgtttattt gttgtaatta aaggagatat tttaatgaac aaaaagaaaa
    55981 gtgcgccatg gcttcaagct ttgcatgcag tgatgccact ttgtataagc tatataccag
    56041 taggattagc ttgcggagta ttgttgcaaa aagttggatt tgatcctctc ttatcaggac
    56101 tgttatcgat tctggtcttt tccggtggcg cgcagttttt agttgcatct atgcttacga
    56161 cgcaagcatc atttgcaacg acattattga tggttttctt tttagaatta cgctatacat
    56221 tattaggagc aagtcttgcc ggttttatga aaaaagaaaa gcggacgttt ttggctgtct
    56281 tttctcaatc actgaatgat gaaaactatg cggtgaacta cttaaagttt tcaactgacc
    56341 cttcctggaa taaaagaaag gctttatatg tcaattggtt ctcaatgact gcatgggccg
    56401 gtagcaatat gatcggtaat tttttcggct cggtgattcg agtagatgct gaccttgtcc
    56461 attttgcttt gacggccatg ttcattttca tgtttatcat gcagatgaaa agcgcactac
    56521 tgatctttac aggtttattc tcaggcgtat taggggtcgt attcatggta ttattccaaa
    56581 atactttcgg attgattgca gcaacagtca ttgcttcatt tgccggattt atgctggaga
    56641 aggcattcaa aaaagaaaaa gctaagaaac taagagaccg cggtaaagat gtcaatgagc
    56701 cgctcttcaa cttcccggaa caggaggcca atcaccatga ctaaactata tttgctactt
    56761 ttagttgctt gtctgttcat cgtggcatat atcccgcgct tgttcccgat gctttatttt
    56821 actcatcgaa aagtgccatc ctggttcagt gactggatga aatatgtacc ggtggcatta
    56881 tttgcggcgt tagcttttaa agatgttttc attactcatg aacatctgga tattgcttgg
    56941 aatatcaaaa tagctgcaat gattctagta gcaggcgtgg cttacaaaac acgttcgatg
    57001 gcgttgtcag ttttgacagg acttgcgtct gtctttttgc tgtctatgct ttaaactgtt
    57061 ttattttatt aaaaacagag attgcgacat aagttgttca aactgatcta tccgaactgc
    57121 atttgcaaca gtcaattctg gatgcaagtt tattcgtagg gataaaaaag attagtgttg
    57181 gcttatgatg caagctcttt tttattttag gaattagaga gggatttgtc cttctatgat
    57241 tttttgctat aatagggaag atgtttattt tatgaaagaa tagttgaggg aatttaaatg
    57301 aataaaaaat tgatgcaagg aacattttgg ctaacctttg ctaatcttct atgtaaagtt
    57361 ttaggggtag tctatttgat tccctggcta agtatgatgg ggaacaatca agatggtatg
    57421 cttgctacga cattgtataa tgttggctat cttccttatg gattgttcct gatgcttggt
    57481 acagtcggat ttccaaatgc gattgcaaaa aaagtagctg tagccacaaa agaaggagat
    57541 aacgctgctt gtcggctgat ttttagaagt acgatcaata tcatgtttgt catcggtatc
    57601 gcctctgctg cactgatgta tttatttgct cctttactag ccggaatcag tccgattgct
    57661 aacgttaata atgggatact agctatcaga agtttatgcc catctttgat tgctatccca
    57721 attcttagtg caatgagagg atatttccaa ggaaaaaatg atctgcggcc ttatggaact
    57781 tctttgatta tcgaacaggc tgtgcgagtg atcgttatct tggctggtac ttattacttg
    57841 cgcgtattga ctgacggaac catattagaa gctgtattga tcagtacagt cgcttctttc
    57901 ttcggtgggt tagctgcgat tatccatatg tatatcgtcg gacgcagaaa agacttcttc
    57961 gaacttaaag attttttgat ttccagccgt tatttcgaaa gagaaaaccg atctgcctct
    58021 gtctctatca ttcgggaaac actaccgttt atttttgtcg gctcagtcat tactatcgtt
    58081 caaatgatcg accaagtaac gatgaaacca ctgcttcact ttttccgtcc agagatcgca
    58141 gatcagcaac tggaattctt attcagccgt gcttctgtca atccaaataa attgacattg
    58201 atcctgatct ccatggtagg aacagtagct ataagcagct tgccaatatt aagcacgatg
    58261 aagaaaaaag atcggataca aatcgaaaaa acagtaggag atagtttttc gattgcgtta
    58321 ttgattcttc ttccttcatt gactggaatg tctttattag ctggaccact atacacattg
    58381 ttttttggct atgatcctga aagtgtcgga tatttccaaa tggctttgct agcttctttg
    58441 tttttctctt tattcacgat cctatctacg atgcttcaat ctttgaatca ccatcgtttt
    58501 gcgatccgat taactgtaga agcaattatc ctgaaagtca tcttccaagt aatcggatta
    58561 ggattagttg gcggatacgg gatgagctta tcaaatggct tagcattcgg tattgtcttt
    58621 gttcgcggct atcactttat gtgcaaagaa taccgaatag cacctttagc aaagatcagc
    58681 aattttttct taaaaacttt ccgctccacc ctgattatgc ttgcagtatg ttttgccgtt
    58741 ttctttttgc tgaatcagcg attgtcaatg gaatcaaaaa gctatgcaat gatttattgt
    58801 gtggctgtag gaagtattgg cggcttagta tttttatttt ctcaattcgg aaaaaatggg
    58861 tttcgaatgt tgaaaaatgt taaacatcat agcagaagca aagaataggt taactaatcg
    58921 ttttttgctg tcacttgaaa aacggcaact cgtatgatta cgcttgtcgt ttttctattt
    58981 tccaatttta gtttcatatc taatcttact tatgatatag ttataaggtc tggaaaaatt
    59041 ctttgaaacg aggaaattaa acacatgaaa cttgcagtag ttacagatag ttcggcatat
    59101 ctgccggaac gtatcaagaa ccatcccgac ttattcgtta ttccaatccc tttgattatg
    59161 gatgggaaaa tttataacga aggaatcgat atcgaagcgg atgaatatta tggactttta
    59221 aaaaacagta aagattttcc tactacttca caacctgtcg taggggaagt tttgggttta
    59281 tatgaggaat tgaaagagaa agggtatgac acgattttaa gtatccatct ttcttcaggg
    59341 atttctggct ttgtcaacac attatttgcg atgaaagatg acatcagcgg agtaacggtc
    59401 attccttatg actctaagat cactagtatg ccgatgggac acatggtaga agctgcctta
    59461 gacttaaatg atgaaggtca gtcccttgat gaaatacttg cacatatcga tcgaatccga
    59521 gataatacct acgcttatct gattgtagat gatttgaaca atttagtacg aggaggacgt
    59581 ttgacaaatg gtgcagcgct gattggcagc ttattaaaaa tcaaaccgat cctgacattt
    59641 tctgaaggga aaattgtttt attcgaaaaa atccgttcaa gtaaaaaagc atttgctcga
    59701 gctgaagaaa ttattggtag aagaaataac gaaatacaac cgccagtcaa actttacgtg
    59761 atacatgcaa ataatttaga agtagcgatt gcggagaaag agaaattgca gaaaaaatat
    59821 ccaaatgcag aaatcgaaat tggtcatttc ggaccagtca ttggtacaca tttaggtgaa
    59881 aaagccatcg gcattgccat ttcagcgcaa taattccttt tattcaaaaa acataccaaa
    59941 aatattccaa catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag
    60001 atggtgtttt tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc
    60061 tataatgtcg catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa
    60121 taaaaggatg aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa
    60181 tcgttgtaga agactatgat aaagttgatg gaagaaatgc attaaatacg gatgcaaaag
    60241 ggctttcact gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg
    60301 tatggcgaca tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta
    60361 ttttagattt agcgatctta gctgcacaag gaagtttata cttccaagat gcttatcgtt
    60421 atgaaaaatt ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc
    60481 gaatgacggt agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt
    60541 acgatacact acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc
    60601 ttttagatga gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga
    60661 gaatttctca ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa
    60721 gaaaccgaaa agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg
    60781 gaattggcaa gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc
    60841 ggggaagaca caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt
    60901 gacctgtttt gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga
    60961 ttctaataag gaacaagacc aagaattact agaagctatt taccaaacag atacacttga
    61021 agctgtactg acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa
    61081 attagcagag ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta
    61141 ttacccttct gaaatcgttc gagcgattga aaaaggatat ttattagaga ttcaagagcc
    61201 gactgtcatc cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg
    61261 gatgttgaat ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac
    61321 tacaaatcga aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca
    61381 acatgcggaa aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa
    61441 aactatgatc caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga
    61501 tgaaactgca aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg
    61561 ggcaaatacc tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta
    61621 taaattatcc actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg
    61681 attgctactg gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga
    61741 gcatacaaaa ggaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa
    61801 tgctgaagaa attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga
    61861 gggagaagag aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa
    61921 agaacgcagt agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc
    61981 tcaagaaatg tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa
    62041 taaagaagcg cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca
    62101 ccgaccagct ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc
    62161 aatagtagaa tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac
    62221 gtaccaaaaa ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga
    62281 tttgagaaat tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt
    62341 acggatcgat gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc
    62401 agcgattgcc attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga
    62461 tacagcagac cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga
    62521 cggatttcaa tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga
    62581 tggtgctgca cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact
    62641 gttgcttaat attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc
    62701 aaaagaagat atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc
    62761 cgcagctatt ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt
    62821 agatattact gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct
    62881 atgataaaat aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt
    62941 taccggtaac aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag
    63001 gagtgaaacg atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc
    63061 tatcctgcta gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt
    63121 gaaaaactta attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat
    63181 caatctccat ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga
    63241 ttcggaacga tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa
    63301 gtgattatgg atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg
    63361 aaaaaatcta aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc
    63421 aaagaaccga ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa
    63481 actggacagt attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc
    63541 gagaaattga aagaagaact attcaatatg atccgatggt ggttggataa agggatagat
    63601 ggatttcgtt tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca
    63661 gagaaccctt tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg
    63721 aaacatgttt ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca
    63781 gaagaaggac cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat
    63841 catatccata tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaactt
    63901 gcattaagcg cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa
    63961 aaccatgatg ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct
    64021 atgtctgcaa aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat
    64081 caaggacagg aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca
    64141 gtagattcga aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca
    64201 ttggatatca ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg
    64261 gaacaatatg ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa
    64321 acgatcaatg tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac
    64381 atgatccgca ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt
    64441 catgaacatc cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt
    64501 atggtaaact taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc
    64561 tggacattac tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca
    64621 ccttatgaag ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag
    64681 ttggactttg cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc
    64741 tctaggacca ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag
    64801 ggttttcaaa cgaggtctga caggtacttg gacatgtttg atatgcatac cgattgatgt
    64861 gtcaccgata tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa
    64921 ctggtcaaaa gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac
    64981 aatttcgaaa ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg
    65041 ttcacaacct tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg
    65101 gatgatccat tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc
    65161 gcttgtgctg catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat
    65221 gtcattaact atttcagtca attggtcttt taattctttt tctgacaact tcattttcta
    65281 aaaccttctt tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta
    65341 taggaaaggc aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca
    65401 ctccagccgg ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta
    65461 agcttcctaa taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag
    65521 caacatatcc ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg
    65581 aaatcaaatc gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat
    65641 aaagaaaagc agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg
    65701 gtacagggat caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga
    65761 tcgttaattg cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca
    65821 cttccggtat gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta
    65881 ctttctgcta ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa
    65941 gaaaaggcac aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc
    66001 tcataatgat caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg
    66061 cctttgatca caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt
    66121 tctgttaacg aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc
    66181 gtaatcatcg taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg
    66241 atcacagctt cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt
    66301 tttgttttta tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt
    66361 ttgataccat ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc
    66421 aaagatatag attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag
    66481 gcgcttagtc cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca
    66541 ccagcaaatg gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg
    66601 aataagagta taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc
    66661 atccaattta aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat
    66721 agttaggact tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta
    66781 gctgttcgcg ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca
    66841 tggacttgct cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc
    66901 ggttgtggag aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa
    66961 tctaataaaa agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc
    67021 cgatctttca aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac
    67081 caatcgccat aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt
    67141 cctttagaca gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa
    67201 tctgtaatcc gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt
    67261 gttttcaaac tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca
    67321 tatacttgaa gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc
    67381 tggttgttta tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga
    67441 tgctccttag cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt
    67501 ggccggtgag cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc
    67561 ataggaaatc ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga
    67621 gagatgtgaa aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat
    67681 ttcgtccgcc atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta
    67741 ccagaagtat gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac
    67801 aaagcaggtt gttccattaa ataaagaata gg
//
LOCUS       DACEQH010000008        61055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.008, whole genome shotgun sequence.
ACCESSION   DACEQH010000008 DACEQH010000000
VERSION     DACEQH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.008"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            212..604
                     /locus_tag="IW833_000509"
     CDS             212..604
                     /locus_tag="IW833_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1306649.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IW833_000510"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IW833_000510"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ1306650.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="IW833_000511"
     CDS             3165..4775
                     /locus_tag="IW833_000511"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ1306651.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="IW833_000512"
     CDS             5067..5936
                     /locus_tag="IW833_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ1306652.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="IW833_000513"
     CDS             6101..7372
                     /locus_tag="IW833_000513"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1306653.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="IW833_000514"
     CDS             7369..8664
                     /locus_tag="IW833_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ1306654.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="IW833_000515"
     CDS             8763..9026
                     /locus_tag="IW833_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ1306655.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="IW833_000516"
     CDS             9471..9920
                     /locus_tag="IW833_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1306656.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="IW833_000517"
     CDS             9977..11395
                     /locus_tag="IW833_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1306657.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="IW833_000518"
     CDS             11395..12255
                     /locus_tag="IW833_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ1306658.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="IW833_000519"
     CDS             12429..13202
                     /locus_tag="IW833_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1306659.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="IW833_000520"
     CDS             13349..13714
                     /locus_tag="IW833_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ1306660.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="IW833_000521"
     CDS             complement(13862..15070)
                     /locus_tag="IW833_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1306661.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="IW833_000522"
     CDS             complement(15089..15367)
                     /locus_tag="IW833_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306662.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="IW833_000523"
     CDS             15560..16216
                     /locus_tag="IW833_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1306663.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="IW833_000524"
     CDS             complement(16658..17455)
                     /locus_tag="IW833_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ1306664.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="IW833_000525"
     CDS             complement(17452..18636)
                     /locus_tag="IW833_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1306665.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="IW833_000526"
     CDS             complement(18661..19908)
                     /locus_tag="IW833_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ1306666.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="IW833_000527"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="IW833_000527"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ1306667.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="IW833_000528"
     CDS             21888..22202
                     /locus_tag="IW833_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ1306668.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="IW833_000529"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="IW833_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ1306669.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="IW833_000530"
     CDS             22887..23531
                     /locus_tag="IW833_000530"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ1306670.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="IW833_000531"
     CDS             23546..23875
                     /locus_tag="IW833_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306671.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="IW833_000532"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="IW833_000532"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ1306672.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="IW833_000533"
     CDS             24863..25687
                     /locus_tag="IW833_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ1306673.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="IW833_000534"
     CDS             25680..26027
                     /locus_tag="IW833_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ1306674.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="IW833_000535"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="IW833_000535"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ1306675.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="IW833_000536"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="IW833_000536"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ1306676.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IW833_000537"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IW833_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ1306677.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IW833_000538"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IW833_000538"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ1306678.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="IW833_000539"
     CDS             31684..32451
                     /locus_tag="IW833_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1306679.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="IW833_000540"
     CDS             32441..34483
                     /locus_tag="IW833_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1306680.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="IW833_000541"
     CDS             34764..34913
                     /locus_tag="IW833_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ1306681.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="IW833_000542"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="IW833_000542"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ1306682.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="IW833_000543"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="IW833_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ1306683.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="IW833_000544"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="IW833_000544"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ1306684.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="IW833_000545"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="IW833_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ1306685.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="IW833_000546"
     CDS             39330..40412
                     /locus_tag="IW833_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ1306686.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="IW833_000547"
     CDS             40412..41215
                     /locus_tag="IW833_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1306687.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="IW833_000548"
     CDS             41285..42025
                     /locus_tag="IW833_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1306688.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="IW833_000549"
     CDS             42036..43040
                     /locus_tag="IW833_000549"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ1306689.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="IW833_000550"
     CDS             complement(43085..44929)
                     /locus_tag="IW833_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306690.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45141..45659
                     /locus_tag="IW833_000551"
     CDS             45141..45659
                     /locus_tag="IW833_000551"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1306691.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45787..46470
                     /locus_tag="IW833_000552"
     CDS             45787..46470
                     /locus_tag="IW833_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ1306692.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46779..46852
                     /locus_tag="IW833_000553"
     tRNA            46779..46852
                     /locus_tag="IW833_000553"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46813..46815,aa:Arg,seq:tct)
     gene            47011..47370
                     /locus_tag="IW833_000554"
     CDS             47011..47370
                     /locus_tag="IW833_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ1306693.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47493..49124
                     /locus_tag="IW833_000555"
     CDS             47493..49124
                     /locus_tag="IW833_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ1306694.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49186..49827)
                     /locus_tag="IW833_000556"
     CDS             complement(49186..49827)
                     /locus_tag="IW833_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1306695.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            50109..50417
                     /gene="rplU"
                     /locus_tag="IW833_000557"
     CDS             50109..50417
                     /gene="rplU"
                     /locus_tag="IW833_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ1306696.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50444..50788
                     /locus_tag="IW833_000558"
     CDS             50444..50788
                     /locus_tag="IW833_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ1306697.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50801..51094
                     /gene="rpmA"
                     /locus_tag="IW833_000559"
     CDS             50801..51094
                     /gene="rpmA"
                     /locus_tag="IW833_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ1306698.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51501..54335
                     /locus_tag="IW833_000560"
     CDS             51501..54335
                     /locus_tag="IW833_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1306699.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            54526..55017
                     /locus_tag="IW833_000561"
     CDS             54526..55017
                     /locus_tag="IW833_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ1306700.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55120..56109
                     /gene="manX"
                     /locus_tag="IW833_000562"
     CDS             55120..56109
                     /gene="manX"
                     /locus_tag="IW833_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ1306701.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56142..56945
                     /locus_tag="IW833_000563"
     CDS             56142..56945
                     /locus_tag="IW833_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ1306702.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56966..57880
                     /locus_tag="IW833_000564"
     CDS             56966..57880
                     /locus_tag="IW833_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ1306703.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57963..58337
                     /locus_tag="IW833_000565"
     CDS             57963..58337
                     /locus_tag="IW833_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ1306704.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58476..58721
                     /locus_tag="IW833_000566"
     CDS             58476..58721
                     /locus_tag="IW833_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1306705.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58893..59957
                     /locus_tag="IW833_000567"
     CDS             58893..59957
                     /locus_tag="IW833_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1306706.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60203..60628
                     /locus_tag="IW833_000568"
     CDS             60203..60628
                     /locus_tag="IW833_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1306707.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60621..>61055
                     /gene="nusB"
                     /locus_tag="IW833_000569"
     CDS             60621..>61055
                     /gene="nusB"
                     /locus_tag="IW833_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ1306708.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKEL"
BASE COUNT        19636 a        10485 c        12851 g        18083 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
    45121 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    45181 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    45241 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    45301 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    45361 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
    45421 atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa
    45481 atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg
    45541 catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag
    45601 catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag
    45661 aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga
    45721 gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg
    45781 agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt
    45841 atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat
    45901 gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga
    45961 cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc
    46021 atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg
    46081 atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt
    46141 gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg
    46201 gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa
    46261 ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa
    46321 gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg
    46381 atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg
    46441 acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg
    46501 attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta
    46561 aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc
    46621 aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag
    46681 ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa
    46741 tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct cagctggata
    46801 gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa
    46861 ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt
    46921 ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa
    46981 ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact
    47041 ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac
    47101 acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca
    47161 ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct
    47221 tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt
    47281 gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct
    47341 ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag
    47401 gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt
    47461 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
    47521 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
    47581 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
    47641 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
    47701 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
    47761 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
    47821 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
    47881 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
    47941 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
    48001 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
    48061 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
    48121 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
    48181 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
    48241 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
    48301 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
    48361 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
    48421 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
    48481 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
    48541 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
    48601 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
    48661 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
    48721 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
    48781 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
    48841 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
    48901 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
    48961 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
    49021 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
    49081 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
    49141 agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa
    49201 aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa
    49261 aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc
    49321 atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt
    49381 aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa
    49441 ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga
    49501 acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg
    49561 atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt
    49621 taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac
    49681 atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag
    49741 aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc
    49801 ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat
    49861 aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt
    49921 attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt
    49981 ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta
    50041 ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc
    50101 tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac
    50161 aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag
    50221 ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg
    50281 ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac
    50341 ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg
    50401 acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt
    50461 taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg
    50521 tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa
    50581 cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg
    50641 ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat
    50701 tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat
    50761 ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct
    50821 acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc
    50881 aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta
    50941 ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac
    51001 tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca
    51061 agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt
    51121 gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac
    51181 gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt
    51241 tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat
    51301 atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt
    51361 cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat
    51421 agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct
    51481 atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag
    51541 aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg
    51601 ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg
    51661 aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta
    51721 cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag
    51781 aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg
    51841 gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat
    51901 taaattgttt gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt
    51961 atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt
    52021 gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag
    52081 gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta
    52141 tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact
    52201 ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg
    52261 tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc
    52321 ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag
    52381 tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt
    52441 tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat
    52501 tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg
    52561 aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag
    52621 caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag
    52681 caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat
    52741 atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa
    52801 tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg
    52861 gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag
    52921 tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact
    52981 ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag
    53041 aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg
    53101 ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg
    53161 aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa
    53221 tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc
    53281 aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga
    53341 aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca
    53401 tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg
    53461 gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa
    53521 aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag
    53581 gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa
    53641 aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga
    53701 tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg
    53761 tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg
    53821 ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa
    53881 atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa actgaattca
    53941 tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca
    54001 tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc
    54061 agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag
    54121 caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga
    54181 caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag
    54241 atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata
    54301 atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat acacttttat
    54361 agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa
    54421 aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa
    54481 tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg
    54541 gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca
    54601 ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt
    54661 ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg
    54721 attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat
    54781 ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc
    54841 ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa
    54901 gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta
    54961 cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa
    55021 gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt
    55081 ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca
    55141 agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa
    55201 gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa
    55261 ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta
    55321 tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg
    55381 gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca
    55441 atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa
    55501 gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa
    55561 cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta
    55621 gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg
    55681 caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga
    55741 ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg
    55801 atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat
    55861 cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg
    55921 tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa
    55981 gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta
    56041 cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca
    56101 aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa
    56161 ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat
    56221 ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg
    56281 gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg
    56341 ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg
    56401 gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag
    56461 ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg
    56521 cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt
    56581 tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg
    56641 tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg
    56701 gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat
    56761 ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc
    56821 taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt
    56881 catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact
    56941 attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga
    57001 tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat
    57061 gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa
    57121 agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat
    57181 tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc
    57241 tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt
    57301 tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt
    57361 agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg
    57421 ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga
    57481 tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt
    57541 cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac
    57601 tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg
    57661 aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac
    57721 aacattacaa gacaacttag accaattgat tcctggttta gctgcattag gattaacatt
    57781 gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt
    57841 cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg
    57901 gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga
    57961 aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg
    58021 gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt taccatgcgc
    58081 gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag
    58141 tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat
    58201 acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg
    58261 aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta
    58321 tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt
    58381 actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata
    58441 tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg
    58501 ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct
    58561 gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg
    58621 aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt
    58681 tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa
    58741 ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt aaaataaaaa
    58801 gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg ctataatgga
    58861 aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa aaattgcgca
    58921 aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac aatcttcgtt
    58981 acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa aaagcatttt
    59041 ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg tttgaaatta
    59101 ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa gaagaactgc
    59161 actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt ttagaagaaa
    59221 tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga gaagtgaaag
    59281 atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg gcatatgatc
    59341 acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat caactagact
    59401 tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt gctagcggat
    59461 tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga
    59521 tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca cgtacattcg
    59581 ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta gatgctcaat
    59641 tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat gcagttgcta
    59701 gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca ggacatggga
    59761 tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag caatttgtac
    59821 caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt ggagtacgta
    59881 ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag
    59941 aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca gtgttcacag
    60001 actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt ttaagctgta
    60061 agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg atcggcggca
    60121 gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta tctcgtgctt
    60181 aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca
    60241 agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg ggattgcagc
    60301 ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg tcactgaatt
    60361 attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag atggcttgaa
    60421 agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag caatggaaat
    60481 gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat ctgaagtcaa
    60541 tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg aagaactttt
    60601 tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt cgggaaatgg
    60661 cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa gatgcgattt
    60721 tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca gagtttgttc
    60781 ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag
    60841 tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa atggatttgg
    60901 tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca gcggctgttg
    60961 ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca cgtaaattcg
    61021 tcaacggtgt tttatccaat gtattgaaag aatta
//
LOCUS       DACEQH010000009        59234 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.009, whole genome shotgun sequence.
ACCESSION   DACEQH010000009 DACEQH010000000
VERSION     DACEQH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.009"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(61..1371)
                     /locus_tag="IW833_000570"
     CDS             complement(61..1371)
                     /locus_tag="IW833_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1306709.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="IW833_000571"
     CDS             complement(1383..2801)
                     /locus_tag="IW833_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1306710.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="IW833_000572"
     CDS             complement(3041..4609)
                     /locus_tag="IW833_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ1306711.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="IW833_000573"
     CDS             complement(4613..4894)
                     /locus_tag="IW833_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ1306712.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="IW833_000574"
     CDS             complement(4891..5814)
                     /locus_tag="IW833_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ1306713.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="IW833_000575"
     CDS             complement(5811..6689)
                     /locus_tag="IW833_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1306714.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="IW833_000576"
     CDS             complement(6676..8013)
                     /locus_tag="IW833_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1306715.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="IW833_000577"
     CDS             complement(8016..9119)
                     /locus_tag="IW833_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1306716.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="IW833_000578"
     CDS             complement(9193..10701)
                     /locus_tag="IW833_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1306717.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="IW833_000579"
     CDS             complement(10667..11359)
                     /locus_tag="IW833_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1306718.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="IW833_000580"
     CDS             complement(11381..12355)
                     /locus_tag="IW833_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1306719.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="IW833_000581"
     CDS             12655..13527
                     /locus_tag="IW833_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1306720.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="IW833_000582"
     CDS             complement(14109..16307)
                     /locus_tag="IW833_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1306721.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="IW833_000583"
     CDS             16539..17531
                     /locus_tag="IW833_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1306722.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17611..19011
                     /locus_tag="IW833_000584"
     CDS             17611..19011
                     /locus_tag="IW833_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ1306723.1"
                     /translation="MSEKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLFKGNVAII
                     QPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWDAYLSLAE
                     NDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGFTIIPCEL
                     IDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPREDAGVFNQR
                     HNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERKVHLLNGP
                     HTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIYADQIKER
                     FLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALLLMYRGDQ
                     VKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEIAAKIDQK
                     GVRSVLQEMKGTMVHA"
     gene            19004..20503
                     /locus_tag="IW833_000585"
     CDS             19004..20503
                     /locus_tag="IW833_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ1306724.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20542..21936
                     /gene="uxaC"
                     /locus_tag="IW833_000586"
     CDS             20542..21936
                     /gene="uxaC"
                     /locus_tag="IW833_000586"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ1306725.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22095..22778
                     /locus_tag="IW833_000587"
     CDS             22095..22778
                     /locus_tag="IW833_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ1306726.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22780..23609
                     /locus_tag="IW833_000588"
                     /pseudo
     CDS             22780..23609
                     /locus_tag="IW833_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            23620..25374
                     /locus_tag="IW833_000589"
     CDS             23620..25374
                     /locus_tag="IW833_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1306727.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25650..25991)
                     /locus_tag="IW833_000590"
     CDS             complement(25650..25991)
                     /locus_tag="IW833_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1306728.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26059..28230)
                     /locus_tag="IW833_000591"
     CDS             complement(26059..28230)
                     /locus_tag="IW833_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1306729.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28441..29298
                     /locus_tag="IW833_000592"
     CDS             28441..29298
                     /locus_tag="IW833_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1306730.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29369..30226)
                     /locus_tag="IW833_000593"
     CDS             complement(29369..30226)
                     /locus_tag="IW833_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1306731.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30211..32913)
                     /locus_tag="IW833_000594"
     CDS             complement(30211..32913)
                     /locus_tag="IW833_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1306732.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(32926..34215)
                     /locus_tag="IW833_000595"
     CDS             complement(32926..34215)
                     /locus_tag="IW833_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ1306733.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34365..35411
                     /locus_tag="IW833_000596"
     CDS             34365..35411
                     /locus_tag="IW833_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1306734.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35413..37566
                     /locus_tag="IW833_000597"
     CDS             35413..37566
                     /locus_tag="IW833_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1306735.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38039..39490)
                     /locus_tag="IW833_000598"
     CDS             complement(38039..39490)
                     /locus_tag="IW833_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1306736.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39487..41211)
                     /locus_tag="IW833_000599"
     CDS             complement(39487..41211)
                     /locus_tag="IW833_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1306737.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41204..41818)
                     /locus_tag="IW833_000600"
     CDS             complement(41204..41818)
                     /locus_tag="IW833_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1306738.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42163..43620)
                     /locus_tag="IW833_000601"
     CDS             complement(42163..43620)
                     /locus_tag="IW833_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1306739.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(43661..44581)
                     /locus_tag="IW833_000602"
     CDS             complement(43661..44581)
                     /locus_tag="IW833_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1306740.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44593..45540)
                     /locus_tag="IW833_000603"
     CDS             complement(44593..45540)
                     /locus_tag="IW833_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1306741.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46019..46738)
                     /locus_tag="IW833_000604"
     CDS             complement(46019..46738)
                     /locus_tag="IW833_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1306742.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(46787..48433)
                     /locus_tag="IW833_000605"
     CDS             complement(46787..48433)
                     /locus_tag="IW833_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1306743.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            48612..49202
                     /locus_tag="IW833_000606"
     CDS             48612..49202
                     /locus_tag="IW833_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1306744.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49294..50799
                     /locus_tag="IW833_000607"
     CDS             49294..50799
                     /locus_tag="IW833_000607"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ1306745.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(50883..52235)
                     /gene="celB"
                     /locus_tag="IW833_000608"
     CDS             complement(50883..52235)
                     /gene="celB"
                     /locus_tag="IW833_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1306746.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52235..52753)
                     /locus_tag="IW833_000609"
     CDS             complement(52235..52753)
                     /locus_tag="IW833_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306747.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(52769..53095)
                     /locus_tag="IW833_000610"
     CDS             complement(52769..53095)
                     /locus_tag="IW833_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ1306748.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53108..55087)
                     /locus_tag="IW833_000611"
     CDS             complement(53108..55087)
                     /locus_tag="IW833_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1306749.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55108..55422)
                     /locus_tag="IW833_000612"
     CDS             complement(55108..55422)
                     /locus_tag="IW833_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ1306750.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            55590..55883
                     /locus_tag="IW833_000613"
     CDS             55590..55883
                     /locus_tag="IW833_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ1306751.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(55961..57073)
                     /locus_tag="IW833_000614"
     CDS             complement(55961..57073)
                     /locus_tag="IW833_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1306752.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57460..57639
                     /locus_tag="IW833_000615"
     CDS             57460..57639
                     /locus_tag="IW833_000615"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306753.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            57833..58003
                     /locus_tag="IW833_000616"
     CDS             57833..58003
                     /locus_tag="IW833_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1306754.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(58427..59167)
                     /locus_tag="IW833_000617"
     CDS             complement(58427..59167)
                     /locus_tag="IW833_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1306755.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        17704 a        12124 c        10364 g        19042 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaacgaagc acctgtaaaa
    17641 gttcttcagt ttggtgaagg caattttatg cgtggattta ttgattggca gattcagcag
    17701 ctgaataacc aacaattgtt taaaggtaat gttgcaatca ttcaaccttt agaacaaggt
    17761 cttggaaata tgatgaacca gcaagataat ttatatacgg tcattttaca aggactcagc
    17821 aatggcgaaa tcgttgatac ttccgagatt atcaccgtag tagaacaaat gatcaatcct
    17881 tatgatgaat gggatgctta tctatctcta gcagaaaatg acgacttggc ctatatcgtc
    17941 tccaatacaa cagaggcagg aatccaattc gtggagactg ataatttaac agatactccc
    18001 ccacagagcc tcccgggcaa actgacagcc tttttatacc gacgtttcaa attaaacaaa
    18061 gctggcttta ccatcattcc ttgcgaactg attgacagaa acggcgaacg tctaaaagaa
    18121 atcattttac gctacgcaga aaaatgggat ttagaacctg cttttgttga gtggttagaa
    18181 aaagaaaatg ttttctgttg tagtttagtt gatcgcatcg tcccaggtta tcctcgcgaa
    18241 gatgcaggtg ttttcaatca acggcataac tatgaagatc aattgatggt caaagcagaa
    18301 ccattcatgc tttgggtgat cgaagggcct aaagaattgg agaatcaact accattcaaa
    18361 caggctggct tgaatgtcat catcacagat gatatgactc cttatcgtga acgtaaggtg
    18421 catttgctta atggaccgca tacagcgatg gtccctcttg ctttgttggc ggaattggaa
    18481 actgtagaag aagtaatgaa agatccttta ttctccgcat atgttgatca gttattcaat
    18541 ctagaattga ttcctatgct ttctttacct aaagatgaat tagccattta tgctgaccaa
    18601 atcaaagaac gtttcttgaa tccttttgcc catcacaaat tagaagcgat ttcattaaat
    18661 agtgtttcaa agttcagcac ccgtctgctt ccagtgttca agaagtatat cgaagaacaa
    18721 aatcaggtac caccgttaat cactgtttca ctagctgcgc tacttcttat gtatcgaggc
    18781 gatcaagtaa agccacatga tgatgaaaag acgataagcg aatttactga tgcttggtct
    18841 gataatggaa cagctattcc tcgattattg caaaacgctg cgctttgggg agaagatttg
    18901 tctcaaatac cgaatgtgac agatacagtt caagaaatag ctgcaaagat cgatcaaaaa
    18961 ggtgtccggt cagtattaca agaaatgaaa ggaacgatgg tccatgcttg aaaccctgca
    19021 accaacaatg aaattaaatc gacgagacag tgtggtcgtc gcattaaccc ctattccaaa
    19081 acaaacacaa ctgacgattg atcagcaaac gatcaccact cttgaagata tcccgcaagg
    19141 tcataagatt gcattgggtg atttgaaagc tggagacaac gtgatcaaat atggctatcc
    19201 aatcggtcat gtgacgacag acgtaactgc cggtcaatgg ctgcacactc ataatgtaaa
    19261 aaccaatctt tctggtgaat tggattatcg atatgaaaaa gatgtccatc caagtcatta
    19321 cctttttgag aaccgaacat ttcaaggtta cttgcgaaaa aatggaaaag tagggattcg
    19381 taatgatttg tttttagtac ctaccgttgg gtgtgtgaat ggcattgctg aactgatcgt
    19441 caaacagttc aaggaaaaac atccagatct gggacagttt gaccatatta cgattttaaa
    19501 gcatccttat ggctgttcgc agcttggaaa agatcatcaa aatactcgag aaattttagc
    19561 ggacgcagta aatcatccca atgccggagg agttttagta tttggattag gttgtgagaa
    19621 caatacagtt cctgaattca aaaaaatttt aggtgaatat gatgaagaac gagtgaaatt
    19681 tctagtggct caagatgttt acgatgagat cgaacaaggt gtggctctat tagaagagct
    19741 tttaacagcg gcggaaaatg accagcgcat atcagtcccg ctctctaagt tgaatgtcgg
    19801 tttgaaatgc ggcggttctg atggtctatc gggaataact gctaatcctt tacttggtgc
    19861 attttctgac tacttgattg ctcaaggagg cagcacgatc ttaacagaag tgccagaaat
    19921 gtttggcgca gaacaagtat tgatggcacg agcggaaaat gaagaagttt ttgactcaat
    19981 cgttgacttg atcaatgact tcaaacagta tttcttgtct tacggggaac cagtttacga
    20041 aaacccatct cctggtaata aagcaggtgg gatcactaca ttagaagata agtctttagg
    20101 ctgcacgcaa aaatcaggaa gctctcctgt cgttgatgtg ctacaatatg gcgaaaaaat
    20161 cagaaaacca gggcttagtt tattgcaagc tcccgggaat gatcttgtag ctgcctctgc
    20221 tcttgcttca tccgattgcc agttggtctt gtttaccaca ggaagaggga ctccgtttgg
    20281 ctcatatgtc ccaacggtca aggtatctac gaatacaact ctttttgaac gcaaaggtca
    20341 ttggatggat tttaatgcag gcgtcttatt agaacaacca atggaagtaa tcttagaatc
    20401 gtttgttcaa aaaatcatcg ctgtagctag tggagaagaa acgaaaaatg aacaaaatga
    20461 cgttcgagaa attgccattt ttaaaaacgg agtaacactt tgattttcat aaaacagtat
    20521 aaaaagaaag aaggaagatt tatgttttta tctgatgatt ttttattaaa agacgattgg
    20581 gcgaaaaagc tttaccattc ctacgcaaaa aacatgccca ttattgatta tcactgtcac
    20641 ttagatccaa aagaaattta cgaaaacaac aatttcagca atctgacaga agcttggtta
    20701 ggaggcgatc attacaaatg gcgcttgatg cgagcttgcg gcgtgcctga agaaaaaatc
    20761 acaggaaatg cttctgattt tgaaaaattt ttagcttggt gtcaaaccgt accaaaaatt
    20821 atcggaaatc cgctttacag ttggtcacat cttgaattga agcgtttctt cggtattgac
    20881 ttgttgataa atgaagaaaa tgcagagaaa atttggacac aagcaaatga aattttggca
    20941 gctcctgatt ttcgccgtcg ggaaatcgca aaaaaatcga atgtagaagt gatttgtaca
    21001 acagatgatc ccgcagacga cttgcactat catcagttgc ttgctcatga agaaccagat
    21061 ttgaaggttc taccttcatt ccgtccagat aaagcactca atattgaaaa agaaggattt
    21121 aatgcatggg tcacactact ggaagaagca gctgaccaaa cgatcaccac ctatcaagaa
    21181 ctgatagaag tcttagggca gcggatagaa tacttccatc aacatggatg tcgtgtttcc
    21241 gaccacgctt tagacatctt acgctataaa gaagcggatg aaacagtatt agaagagatt
    21301 tttcaaaaag cacgagctaa caaactatta tcttcagatg aaatcgatgc ttatcgtaca
    21361 gaaacactga tacatttgac ccacttctat cattctcata attggacgat gcagctgcac
    21421 atccatgctt atcgtaactg taatacacaa atgttggaaa aattagggcc cgataccggc
    21481 tatgatggaa tgaatgatct ttctttgact attcctctgc aaaaattatt gaaccgtgca
    21541 gaagaaacgg atcagttgcc aaagacgatc ctttattcat tgaatccgaa tgactatcct
    21601 gcgattttag ctttgatggg ctgtttccaa aaagaacaga atggaaaact gcagttaggt
    21661 tctggatggt ggtacaacga tactcgagct ggtatgcgcg atcaattgac acaatttgct
    21721 gatggtagtg ctttggggaa ttttgtcggc atgctgacag attcaagaag tttcctgtcc
    21781 tacactagac atgaatattt ccgtcgtgtt ctttgtgaat ttttaggcga aatggtcatg
    21841 cgaggagaag cacctgaaga tgaacaactt ttaggaagtc tagttcaagc gattagttat
    21901 acaaatgcta aaaattactt tggctttttt gcctaattga ttaatagtaa cagtaacgaa
    21961 tcaaaaacag gatagtgaaa taacggaatc cgtttatcca cgttttagag aagaagacgg
    22021 accaaatttg ctccgtcttc ttctcttgct gttctgcttc attgcctata aacctttatg
    22081 aaagaagaga taacatgcct actatccata ttgccggtga ttctactgcg gcagaaaaag
    22141 atgaaaacaa gcgtcccgag acaggttggg gagaaaaaat ctctgcttat ttttcacccg
    22201 agatccagat caataaccaa gcaaagaatg gacgcagcac caaatcattt atccaggaag
    22261 gaaggttagc ccgtctagct tcacagtttc agccgaatga tttcttattt gtacaatttg
    22321 gacataacga ccagaaaatc caagaagaaa gaggtacgct tcccttcggt agctatttaa
    22381 aaaatctgca gatatttttt gaatgcgcaa aaaaagcgaa cgtccaaatg gtcttactga
    22441 catctgtgac gcgacgagat tacttagaaa acggaacatt gaatccagat atacttggag
    22501 actatccaaa agcgatgcgt gcatttgctg agaaacacca tattcctctt ttagatgtgt
    22561 tctcaagatc acaagaattg ttccagacct tctcaaaaga agagacaaaa aaattctacc
    22621 tgcatcttat acctgatact tgtaaaaatt acccagaagg actaaaagac aatacccact
    22681 ttagtccaga aggcgctgat aaagttgccc gaataattgt tgaattgata aaagaatccc
    22741 gtctgcctct tgctaattat ttacaaaaag gagtttgaga tgtttccatt aaatttaggc
    22801 atccgtgccc acgatttaga tgttcgaaat cgtgaagaaa tcgttcaaaa agtatcacag
    22861 cttcacttgt cccatatcca atttgcacca caaaaagcat ttcctgaaca acttaaatta
    22921 actgacatga cacttgggag tgctgcctat tttggtgaat acttccaaaa aaacaatatc
    22981 gaactttcca ttttaggatg ctatatcaac ctatccagca aaaatcaaga agttcgtgca
    23041 caagcattgg caactttcaa acagcaaatt gctctgccta tcaagccaag atgattgcta
    23101 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
    23161 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
    23221 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
    23281 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
    23341 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
    23401 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
    23461 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
    23521 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
    23581 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
    23641 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
    23701 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
    23761 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
    23821 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
    23881 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
    23941 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
    24001 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
    24061 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
    24121 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
    24181 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
    24241 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
    24301 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
    24361 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
    24421 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
    24481 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
    24541 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
    24601 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
    24661 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
    24721 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
    24781 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
    24841 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
    24901 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
    24961 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
    25021 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
    25081 tctggtggcc aaaagcaacg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
    25141 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
    25201 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
    25261 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
    25321 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
    25381 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
    25441 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
    25501 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
    25561 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
    25621 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
    25681 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
    25741 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
    25801 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
    25861 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
    25921 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
    25981 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
    26041 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
    26101 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
    26161 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
    26221 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
    26281 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
    26341 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
    26401 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
    26461 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
    26521 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
    26581 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
    26641 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
    26701 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
    26761 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
    26821 atatcctttt tgtaaaccga tctcatgcaa aagccataca gtaggcaaat tcaaactttg
    26881 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
    26941 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
    27001 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
    27061 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
    27121 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
    27181 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
    27241 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
    27301 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
    27361 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
    27421 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
    27481 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
    27541 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
    27601 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
    27661 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
    27721 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
    27781 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
    27841 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
    27901 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
    27961 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
    28021 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
    28081 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
    28141 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
    28201 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
    28261 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
    28321 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
    28381 cttaaatatg ttttaaaaat ggtacaatta gtctgcgatt gcactgaaag gagtacactt
    28441 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
    28501 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
    28561 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
    28621 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
    28681 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
    28741 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
    28801 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
    28861 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
    28921 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
    28981 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
    29041 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
    29101 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
    29161 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
    29221 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
    29281 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
    29341 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
    29401 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
    29461 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
    29521 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
    29581 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
    29641 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
    29701 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
    29761 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
    29821 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
    29881 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
    29941 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
    30001 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
    30061 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
    30121 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
    30181 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
    30241 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
    30301 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
    30361 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
    30421 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
    30481 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
    30541 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
    30601 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
    30661 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
    30721 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
    30781 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
    30841 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
    30901 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
    30961 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
    31021 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
    31081 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
    31141 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
    31201 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
    31261 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
    31321 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
    31381 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
    31441 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
    31501 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
    31561 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
    31621 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
    31681 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
    31741 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
    31801 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
    31861 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
    31921 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
    31981 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    32041 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    32101 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    32161 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    32221 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    32281 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    32341 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcaggtcctt tccaccacat
    32401 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    32461 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    32521 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    32581 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    32641 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    32701 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    32761 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    32821 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    32881 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    32941 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    33001 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    33061 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    33121 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    33181 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    33241 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    33301 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    33361 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    33421 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    33481 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    33541 ataacggcaa gtatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    33601 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    33661 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    33721 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    33781 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    33841 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    33901 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    33961 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    34021 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    34081 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    34141 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    34201 atctttataa gccactaata aaaccttctt tctatttgat atatctttaa agattatact
    34261 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    34321 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgaat caaccattgt
    34381 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    34441 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    34501 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    34561 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    34621 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    34681 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    34741 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    34801 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    34861 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    34921 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    34981 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    35041 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    35101 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    35161 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    35221 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    35281 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    35341 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    35401 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    35461 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    35521 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    35581 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    35641 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    35701 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    35761 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    35821 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    35881 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    35941 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    36001 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    36061 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    36121 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    36181 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    36241 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    36301 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    36361 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    36421 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    36481 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    36541 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    36601 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    36661 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    36721 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    36781 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    36841 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    36901 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    36961 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    37021 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    37081 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    37141 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    37201 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    37261 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    37321 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    37381 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacaa agaatatacg
    37441 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    37501 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    37561 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    37621 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    37681 accgtttatt gagaggttgt gaaatcagcg ttctgacctg agtattagag agacaaatgg
    37741 caaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca gctgattgaa
    37801 caccgtttag tggagaggtc gtgaaaaagc tgtttagttt cgagtattaa gaaaaatttt
    37861 gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa actggctttt
    37921 gaactccgtt tatcgagagg tcatgaaaaa gctgccttgc ctcaagtaat aagaacagcc
    37981 caaaattaga tttctagaca attttttata agttatttca ttacattggc tttttctttt
    38041 atgattctcc actgttttct cggtactctt taggggatct gccgacgatt ttcttgaata
    38101 atttagagaa atacgtggtg ttgttgtagc caatctcact tgctatctca ttgatgtttt
    38161 gattactgtg caaaagcagt tgtttcgctt tttccatacg taaatggttc aaataagctg
    38221 aaaaactttt tttcgtttct tttttgaaca gctgtcctag atacatgaca tttaaatgca
    38281 ggttttctga cacttctttg agtgtaagtg gttcttgata atgctggtgc aggattacga
    38341 ttactttttc cacattgtca ctgtactttt tctgtctctg atgctttttg atgaatgaca
    38401 gaagtagctg ctgaagttct tgcaccgttt ttgcctgatg gattttttcg attccttgta
    38461 aatattcctc atcttccaga tcaatcaatt cccgttgaat ctccataaac agtaaaaaac
    38521 tggaatatcg tatatcttcc ggcatcatgg ctgctaactg aaactgttca aaaaaatcag
    38581 tgatcatttt ttgagcgact tccaattgtt tattttgcag cacgcgtctg aaacttgaaa
    38641 aatcgatggt ttgatctttt gtttctgctt gtactgcgta aaaaacatgc tcgtctttat
    38701 ctccataaaa ttgatgcaac tgtagattgt ctttcgcttg ttggaatgtt tcagggattt
    38761 tgtccaaaga atctgtctct tccccgatac tgatcagcca ttcctgatca ggaaagtttt
    38821 cttgaacaaa acaacagaag ccatcaactt catctgcctc caacaacaac gtgatcaata
    38881 cacggtctcc gtaatttcgt tgatagtaaa agatttgttg ccgttcttgc aaccatctat
    38941 tgataagagt atcatgagtt cttgccagtt gaatcaatac tatcctatgc tgcttctcac
    39001 ttatttgtcc tattatttct tcttcactta tttcatccaa ttcttcattc aaccattgac
    39061 tgaataacag ttcttgatag atttcttgtt gatttttttg ttcattttgc tgatctaacc
    39121 gtgttttgat tttcttgagg ctttcaacca attcgaactt attcacgggt ttcatcaaat
    39181 aattcacggc gcctaattgc atacctcctt tcaaataatc gaattcttgg tatcctgata
    39241 aaatcataaa ttcaaaatta tgacgttctt tttgagccgc ttcaataaat tccagtccat
    39301 ttatttctgg cattgtcaca tccgttacta ctaaatcaat ctgtcgctgt tcaagtacag
    39361 ctagtccttc tttggcattc tctgctgttg ctactatttg aaagcctaaa ctttgccaat
    39421 caattatctt ttttaaccca ttcaaaatca tgtattcatc atctaccaat aacacttgat
    39481 acacgctcat ccctccttga atgtaataga aacggtcagt ccacccgtct cattattctc
    39541 aagcgacata taataactgc tgccgaagct ggcacgtaaa cgttcattca cgttttgcag
    39601 accgatagaa ccgtgaagtt ccactttagg atgagacagt ttttcttcaa tctgttttag
    39661 ccgtttttca gtcatccctt ttccattatc tttgatgatg atccttaccc gtttgccttc
    39721 ctgcaagact ttgacactta gcgcattatc gaatcgagta aaatcaatac cgtgtttaaa
    39781 ataattttca actaatggct ggatcacaaa tttaggtact tcgatctttt ccaaatcagg
    39841 atcgatcata aaatgatagg ctactcgatt aggataacgc atttgataca aatagacata
    39901 tttttcgcaa aagtccagtt cttcttttaa agtgatcgtt ttctcttgat tcgtattgtt
    39961 tcgaagcaaa gcggaaaacg catacacaac atctgctaat tcttcacttc cttcgctgat
    40021 tgcatacatt cggatatatt ctaatgtatt atataaaaag tgtggattga tctgtgcttg
    40081 aagcgcccgc atatgtgcgt cttgctgttt gatttctaat ttgtagatgt cttctacaaa
    40141 ctgattgatg ctatccaaca tttcgttgat accgattgaa agttccttca gttcatattc
    40201 cgtgtttgtt atgtcgatcc gcgtctctaa attccctgct gctgtttgat ccatggcctg
    40261 catgacgata gatacttgct gtgaatatcg tttgaatgtt ttataaagaa agataagcag
    40321 tatattgtct aaaataatga taccaatgat cagcggggca agattttttc ccgtttcgat
    40381 ttttacactt tttttatcta gtacagctaa tatacgataa tcatcagaca gttcttggtg
    40441 ttgaacaaaa ttgttttttt ccagatcgtc aatcggcaat tgtgaatttt tcttcagact
    40501 ctctgtgatg atcatttctt gtctttcaaa cgtttcttta gctattttat tatcatggaa
    40561 tgtgtatagt cgatttgtcg ttccagaaat catgtataaa gagagtccat tgaatgacgt
    40621 tagattttct aaaatcatat ctagttcttt cttttgaaaa ccaataaaaa tacttcctga
    40681 ctcagctcct ccttgtgtga taggcaactt gatataaaag gcgtctagta attttggtgt
    40741 cccctttttt atttccccac ctttgttttc tctagaagat tcataatact ccggtagttg
    40801 attcaacacg atatatatcg cacttagttc ttcataattt gcgtagagat ctttgatttg
    40861 atttgggaaa gaaactaatt catcggtact gttttgctgt tcgtatacat acatcagata
    40921 ctgatctatg ggttttgtta agtaggtcgt cacattttcg atcttttcgg aattccctac
    40981 aagctggttt ttgatgatcc tactcaaggt tgttttatca ttcagcattc tgcttgtgct
    41041 ttgaacggca tcacttgtca tgttttctgc ctgtttttga ttttgttccc aagtatggat
    41101 actaaaaata acggtagcag tcatcgttac accgatcatg ataaccgcgt atttccgcaa
    41161 cagacgattt agtaaggttc cttttgaaaa tagaatccga aacttattca taactttcca
    41221 actgtttatc taattcttca cgccaatgtg ttgtcatcgt accattccaa accgtcaaaa
    41281 gaccaatgac accaaacagg atcaatcctt tgaattgcca agtcacgcct aaaattatac
    41341 cgctaccaat aattattttt agaaaagacg aaaagctgga aaaaaccgaa ataaaactta
    41401 gctttagcag attaggaaca gagatcgtat attcactatc taaaatcata ctgtattgat
    41461 aagtagcaaa taggcagagc aaggcaaaaa gtaaaataaa atcaatagct aaaaaaatga
    41521 gccctttgat ttgaacagat aaatataaat tgtaactcaa taaagcagta ccagataaaa
    41581 ataaactaaa tagcagattg cctcttaaaa aatttctttt atatatattc cagcctcttt
    41641 ttattgtgat ttctttatac tcgaatccgt acaaataaaa tagttcattg actgttttcc
    41701 atgccgcacc taatccaaag atgatcccgc cggcaaatgt atatatccag aaatacaagg
    41761 ttagttttat aatgacccag atccggataa ataactcttc caaaaccttc cctaacatat
    41821 cttctcctcc aaacattaag tttatacttt ctcattatac aaaaaaaggt tgggcaatca
    41881 gcaccttgac ctgattatta gagaaacaaa cggaaaaata gttcctcata ttttgtcgtt
    41941 ttgtgagcga ataccgaggg ttagctgatt gaacaccgtt taccaagagt ttgtgaaata
    42001 gccgcttagt ttcgagtagt aagaaaaatt ttgtgaatat ggctttccac atttttacat
    42061 atatttagct taataccgag aaactggctt ttgaacaccg tttattcgga acaagaggtt
    42121 gtgacacgtc ttttgtcaca acccctttgt ctttattcat tattattctt gtcggaactc
    42181 atcatattgt ttttgcattt ctttcaatac cttatcatag ccagccttgt ccaaagcgtc
    42241 tttcaatttc ggtaatgttt catctggatc aacagtccct gtattcaatc catctaaata
    42301 ttgattcatg acattagaaa tattacttaa ttctgttttg acactgtcag tgttgaagct
    42361 gaatcctaaa atcggtgaag tttctgcatc agcaatcgat tgatcacgtt tggcaatcat
    42421 gtcatccgta atagactctt gtgtatatag aatcttgttg tttcctgtat tccaagcaga
    42481 catatgtgta tttggctgat aaccatctag caatttgatt ttatcatctg aaccatcgac
    42541 tttttcccat gcttctccct cgatccccca aacaagccca ttcaataatt ctggatcact
    42601 gttaagcagc cctagaagtt ctactgattt ttctttattt tttgaagtat ttgatacgac
    42661 aaaatttgcc atttgtgctt gagctgtaga ttttagtgga acagtaattg cttttgagac
    42721 aagttcttgt tttgctgcat ttgtcaaaat tgtatcgcca taatcatacg gtccttgtgt
    42781 ttctccacgc attagccaag tatttccttc tagtggataa tccttattgc tcgttgcagc
    42841 gtcacttgga atgagtcctt gtttgtacca gctatgcatt gtacgtaaca gtccgatata
    42901 cgtttcatta tcgtattggt tgatgatttt cgaatcatct ccatccagat caatagcaaa
    42961 tggtaacgta ttccctaaag gataatcaaa gttcccttgg actttgaagc cttgtccaat
    43021 ggcaaaagct gccgtgtttg gttctttttc atggaattcc ttcaagactt tttctgcatc
    43081 agcgtaagat tggattccat caattgaaag atcgtattta tctagtaaag ctttgttaaa
    43141 agtaagtact tgttgagcaa agacatttcc attgactggg aaagcatata attttccatt
    43201 gaccagattt cctttaatat atgcttcatc taaatcatta taagcttttt cagcgtactt
    43261 aggcgctaat tcagtcaaat cagcaaaggc tcctttttgg gcattggaaa catagttgtt
    43321 cgcaaaggcg atatcataat tttccccgga agaaatgatg acgttcattt tcttttcgta
    43381 atcgccccag ccaatgtaat tgatattgac ttttacaccg atctcttttt cgattcgttt
    43441 gtttgctact tccatcaatt gatcgaaatt atctggttta tctccgattt gatacatctg
    43501 caaagtgtcc gaattgctag cttctgcctg gtctttagaa ccagaacctc cgcatgctgc
    43561 taatgttccc aacaacaacg ctgaaacacc tgtaaaggct agtttcttcc ataacttcat
    43621 ttttcttcct cctaatttta tgatttatat ttttgtttct ttattctttt actccgccga
    43681 ttgttagacc tttgacaaaa tgcttctgga aaaatggata gctgatcgca ataggcaacg
    43741 tcgagatgac tacgatcgcc attctggcag attctcctgg aactgaggct aatccgcctg
    43801 tcaattggct tcctgctcct gaattttgtg tcagatattg aatattactt tggattttca
    43861 tcaaaagata ctgtagtgga actagcttat catcttggat gtatagtaaa gcattgaacc
    43921 agtcattcca gtaacccaat gcggcaaata aactgattgt cgcaatacct ggaacagcta
    43981 acggtaaaac gatctgaaca aatattcgca gttcgctagc tccatcgatc cgtgccgatt
    44041 caataatcga atcagagacc gaacgtttaa agaatgtgcg catcacaata atattgaagg
    44101 gactgacagc cattggcaag atcaatgccc aaatcgtatc ttttaactga agaagatttg
    44161 tcataacaag ataattggcc accatccctg gtgagaacaa catcgtaatc aaacaaaaaa
    44221 cggtaaaaaa acgacggaat ggaaagtttg accgtgaaat cgcataagca tataaagaag
    44281 tagccgtgct attgatcagt gtacctaaaa cagtaacaag tacagtgacg aaaagcgctt
    44341 ggaaaatttt atcctgcatc tggtcgaaca agtacgtata accaaatata ctgaactttt
    44401 ctggccaaaa actgtacccg tgagtcgaca atatggactc atcggtaaaa gagatggcga
    44461 taacaaatat aaatggcaaa atacaggaaa tagcaaagag tgcaatcatt atattagaga
    44521 aaaaattagc cgtcttattg aaagagcgga cttggactct ctccactttc ttttttttca
    44581 tttttttcct ccttagaata atgcagaatc gctatcaaaa cgtcgggcga tcgtatttgt
    44641 gatcatcaac aagacaaagc cgacaactga ttggtaaagt cctgcagcag ccgtcatacc
    44701 aaaatctcct gtggatgtca ggccattgta gatatacgta tccaaaactt gagtcacatc
    44761 atataatgca ccggaattcc taggaatgtt ataaaacagg ccaaaatcag ctcggaaaat
    44821 gtttcctacg gctaaaatcg ttaaaatcgt cattagtgga ataagttgtg gtatcgtcac
    44881 atgtttgatc tgctgccatt tgctagctcc atcaaccata gcagcttcat aataagtcgg
    44941 atcgattccc ataacagatg caaagtaaat aatgctgtta taaccaatcc ctttccatgt
    45001 accaatgaac accaagataa acggccaata tttcggttca ttataccaat tgacagcttc
    45061 tcctccatgt ccagtgatcc actgattgaa tattcctttg tcaggactta aaaaagcgta
    45121 aacaaagtaa ctgataatga cccaagatag aaaataaggt agtaaggaca tcgtttgata
    45181 aactttaacg agtcgtttat ttctcaactc gctcataatg attgcaaata cgactgagat
    45241 cagcagattc aaaaggataa atgctacatt atataaaatt gtatttttag tgatgagaaa
    45301 tgcttgatcc gaagaaaata aaaatttgaa attttcccaa cccacccaag gactttccct
    45361 caagctagcc aagaacccat ctgctgaaat atgaaaattt ttgaatgcta cgacattcgc
    45421 cattacaggt atgtaaaaga aaaaaatcat ccaaagcata cccggtacag ccatcaggac
    45481 aagtgcttta taacgccata gattcataaa aaaatgattg aacccctttc gttttttcat
    45541 ctaaaaaccc ctttcgattc attcgcaacg taattatagc gctttcaata atgagaataa
    45601 ataaacaaag tatagggaaa atgaaacaaa gtataggaga ggttgtgaaa tcagcgtttt
    45661 ggcctgagta ttagagaaaa aaacgggaaa atagttcctc atattttgcc gttttttgag
    45721 ctaataccga gggtcagctg attgaacacc gtttatcaga ggttgtgaat tcagcgtttt
    45781 ggcctgagta ttagggagat cgtgtcctta ctatctaact ctgagtcaca aggaacaatg
    45841 gggactgtaa caaaagtctt gtcacagtcc cttattccga acaaacggtg ttccagaagt
    45901 agcttctacg gaaataagcc gaaatttcac aaaaaagcca tccgaaagtc agacggacaa
    45961 gtctgctttt cagatggcgc tacaactact ctgcttattc ttttagctat ccattcaatt
    46021 atttttctct cacttgttct agctcttctt tggtcatggg tcgatattgc cctttattca
    46081 aatctttagg cagccaaaat tttcccatac ggattctttt taaatataag acttcttttc
    46141 ctactgcttt gaccattcgc ttaacttggt gaaactttcc ttcatgcaaa atcagacgga
    46201 taattgaagt ttctgtctct tgatctactt gatcaatttg gagttttgcc ggcaatgtct
    46261 gctcatcgcc atcaataatc agtccttctg caaataaacg gatatcttct tctgtcatta
    46321 taccgctaac ctcagctaga tattctttct ccacatgttt tttaggtgat agtaactgat
    46381 gggcaaacac cccgtcattc gttaaaatca agaggccctc tgtgtcttta tctaatcggc
    46441 cgactggaaa aagatcttcc cgataatcct ggtcttccaa taaatcgatt acggtttgat
    46501 catgattatc ctgagtagca gaaatgactc ctgcaggttt atgcaaaatg tagtaaaaat
    46561 ctttttgata cgtaatgatt tctcctaaat aagtaaccgc atcttcgaat tcatttactt
    46621 gcgctttatc gcttttcacc acttgtccat tgacttcgat cgttcctttt ttgattaatc
    46681 ctttgacttc tttgcggctg ccaagtccca tatctgctaa aaacttatct aaacgcatgc
    46741 gtttccccca tttcaaaaga gcatcaatct agctcttctg attcacttat tttattttta
    46801 atttatgtcg taaacgcgct attcctggtc ctagtagtct ttctgccaag cggattttca
    46861 aagctaaata accatacact aaagccccta tacctgctac tacaagaatc aacaatagag
    46921 actggaattt gctatcttcg tttaaaaaag caccaaaaat catctttgtg atccacgaag
    46981 ccaaaagcat aatcaaggta agaataaaga tcagcaatgt cctgcgccag acaaaacttg
    47041 gtttaaagcg ggctacttca tggattctct ttaagatcaa ggtacaagac acagcaaatc
    47101 cgatcattgt cgccaataaa gggccgtata cttcaaataa acgaatggct gggtattgaa
    47161 gaaggagttt cactagaaac ccaataacca aataattaat tgccgccttg ttttcaaaca
    47221 ttccttgcag catgttggaa actagcatat atagtcctag gaagagtcct acaaaacttg
    47281 cttggatcag tacattactt cctagagaat ccggtgtata aaataatgta tttagcggat
    47341 aggcaagtaa cataacacca aatgtcgccg gaaacataaa aaatgaaaac aactgtaaat
    47401 ttccgcttgt caattttgcc agacctactc gatctttcaa cgtaacagct tctgtaatca
    47461 acggtaaccc agtagacgcg atcgatgttg ccaatgcaat aactaccatc gtcaacttat
    47521 ctggattggc actaaaaatc gaaaaaagat ccagtaactg tgcattggaa tactcggtgg
    47581 atgcagacat gatcttcata aaagtgaact gatcgactaa tttgaagatc gttacccctg
    47641 aacccacaat aataaacgga atcgcttctt taacggtatc taacaacaat tccttcgttg
    47701 caatatttat tttgttttcg ctgtagtgaa taaacgccga tgtatacgct tgatgtttct
    47761 tcaaaaaata caaaaggaca agaatacttg ctagcatacc gataaatgcc gcgaaagttg
    47821 attgcgtgac cgccgtcaca tagtcaccat ccatgacttt catgatgatg aatgtagcca
    47881 atagcatata aaaaatacgt gccacctgtt ctacaatctg cgacagcgca taaggcatca
    47941 tttcttgatt gccttgcaaa taccctcgta tcacactcat acaaggaaaa accaatacag
    48001 cgatactcaa agaacgcatc gttggaatca gttcttctcc gccaccagaa gcatgagcta
    48061 gccaaggaga ggcgatgtac atgaacaaag caaacaacag gcctaacccg cccatcatct
    48121 gtaatgcacg gataaataat cgtcgtgaag tgccatattc attcaatgaa ttatagcgtg
    48181 cagtttgttt tgcaatcgca gcaggtattc ctgctgtaga gatcagtaga aacaatgcat
    48241 atatattgta tcccatattg aataatccat tggcagcctt agcattttcg cccatccaag
    48301 cataccaggg aatgatatag acagcgccta gcaatcttga aatgatatta ctggcggtca
    48361 tccaagcaga gcctcgtgcc attttttctt gcggtgttaa ttgatctaat gctggttttc
    48421 tttgattatc catcggtcag attccaactt tctacaaaac attactatca ttttaattgg
    48481 taaaacagaa actggcaatc gattttatat aacttttata agaacaattg tttttttgaa
    48541 ttcggtttcg tttttttggt aaagtaaaga aatatacagc cttttatggc taacatttat
    48601 aggagatatc aatgacacaa gaaataaaaa aactacccgt tatattttta ggtcttttct
    48661 ttatcacagt tggattgaat tggttcttgc tccctcacga tatagcttcc gccggagtgg
    48721 gcgccatcgg acatttagtt gaaacggcct tttctatcca acgctcagtt actgtttgga
    48781 gcatcaatct tatgatgctg cttctagccg ctgtcctatt aggaaaatca gtatttttta
    48841 agactgtagc gggaagtctg ctttttcctg ttgttttaga gattgttcca aaagtggaaa
    48901 tgatctcctc acatttcttt tcactgattt caggaagtct tttgttcagt cttggggttt
    48961 acacattgta cacagtcggg gcctcaaatg gcggtataac gataccgccg attattttcc
    49021 acaaattttt tcggcttcca atgccgcaag gacttttact taccaatagt cttatcgtct
    49081 ttttgaatta ttatgttttt ggattgttgg agacactatt cgttctccta tctattacag
    49141 tcagttcatt gtttatgaaa atactcatcc gcttgtcccc tgtgtcaaga aaatttgaat
    49201 aaagctggat tttgtcatag cggaaacaaa cgatatatta aaaagagtgt gctataataa
    49261 accaagaaat tgaataaagc gagggatcaa tcaatgagct tctctttaga agacatccgt
    49321 catttactat taaaagaaaa tctattaaaa gaatttgttt caacaagagg ctggcatctt
    49381 gatgtaccag aaaaagctga atttcttcag ttaagttatg attcaagaaa agctgatgct
    49441 tctactttgt ttttctgtaa aggaatgaat ttcaaagaag aatatttgga ttcagcaatc
    49501 gaacagggta ttcaatatta tgtatcagaa caaccatacg aaaattcagc agtaggaatc
    49561 atcgtaacgg atatccgtaa agcaatggcg cttcttgcta tggcttttta cgactatccg
    49621 caaaataaat tgaaagtgat cggattcact ggaacaaaag gtaaaacaac tgctgcgtac
    49681 tttacaaaag caatcttgga tcacacaaca aataaaaaaa cagcattgct ttctactatg
    49741 aatacaacgt tagatggcaa aacatatttc aaatcccatt tgacaacgcc tgagtcatta
    49801 gatttatatc gtatgatgtc agaagcagtt gaaaatggta tgactcatct agtgatggaa
    49861 gtttcttcac aagcttacaa aacacaacgc gtatacggct tgactttaga tgtcggaatc
    49921 ttcttgaata tctcgcctga ccatatcagt ccgatcgaac atccgacatt tgatgactat
    49981 ttctattgca agcgacaatt gatcttgaat tcaaaaacag ttgttttaaa tcatgaaagt
    50041 gactattttg atttacttag ggaaacagcc gatctattcc agattccaac gatcacttat
    50101 ggtcgttcgg aagaaagcaa ttatcaagtg atccgttcag ataaaggtac tcacggtttt
    50161 actttatctt ctcatgaaga tcgcttagct atctgcgata ctgcttatga tattttgctg
    50221 gctggaggat tcaaccaaga aaatgcagcc agtgcgatca ttgccgcagc actaacaggt
    50281 gcctccagtc aagatgcaca agacggatta aaagaagctc gtgtaccagg ccgaatggat
    50341 caattgatcc aatccaatgg agcgcatgtc tatgtggact atgcacataa ttatctaagc
    50401 ttgaaaacat tgctgaaatt tgccaaaaat gaacatccag atggccgtgt aattgttgta
    50461 ttaggcagcc caggaaacaa agcaatctcc cgtcgacatg attttggaaa agtgctatca
    50521 gaaacagctg acatcgcctt tttgacggca gatgacccag catttgaaga tccgaaaaaa
    50581 atcgcggaag aaatcaatga agcaattact aatccagatt tgatcgttca ttatgaaatg
    50641 gatcgtcctg aagcgattcg acgcgcttta gcagaaagcg ctccgcaaga ttcagtcgtt
    50701 attgcgggaa aaggcgtgga tccctatcaa aaaatcaatg gtgtagatga accttatgaa
    50761 ggggattatg caatcgtgaa gcggttgatt gaagaataat ggtatttata tactagtggt
    50821 ttcccatttc taagaaaaaa agggactgtg attttttgtc acagttcctt ttttcttaga
    50881 aatcatttta acttttcact agctaattct gctgcctttt ctctttcttc atttaataat
    50941 ttattatcat agaatttgat aaatgggaac cagataatga atgcgatgat tgcacaaatg
    51001 actgctaaca cagcagcttt ccaatcacca ccactaccaa caaatgctcc gatcccgact
    51061 ggtgaaggcc acggcatctg tgcaagcatc ggtttgacga tttccagccg aatggcaaaa
    51121 taagcgagtg tagcagaagc catcggagct aagaaaaacg gaaccgctaa atagggattg
    51181 tagacgattg gcatcccgaa aatgattggt tcattgatat tgaataatcc tggtacaagt
    51241 gaggcttttc ctaacacttt cagctgatca gatttcgcga ggaatgcaat aaagatgatc
    51301 aatcctaaag tcgcacctga tccaccaacg gttacataag aattgttgaa ttctcctgcc
    51361 aacggataat ttgcgccttg tgcgttagct gccatattag ctaatacgat cggggtcaag
    51421 aaagaagtga tgatattcgc tccatggatc cctacgatcc acaatgcatg gatcaagaaa
    51481 taaatcacca tgacaccaag ccatgtattc gtcaactgcg taacaaaccc aaatgggata
    51541 gcaatcattt tgaagatatc cgttcctagt gcaactaata atccattgat gatcatcacc
    51601 acaaacgcaa tgacaaatgt tgggatcaga gcagtgaaag aacgagaaac tcctggcgga
    51661 acaacatctg gcatttttat gacccaattt ctcttgacac acatacaata caattcgacc
    51721 gcaataatcg acataataat agctgtaaag attcctgaag ttcctaaacg ttcgacaaaa
    51781 gagcccatcc taataccatt tactacagtt tctgtctcat tgatagaagc aatcaagctg
    51841 attttaccat cagtcatgat caattcaggg atacacataa agaatgcaaa aacagatagt
    51901 aatgcccccg tgagcggatt aacatttaat ttttgttcat ctcgttcaat tgacgttaat
    51961 tcataagcaa acacaatggc aaagtaaata gataaaatcc ccatcgtcat tgtattgacg
    52021 atcatgtaca agtcactaac tttgaaaaaa gttgcattaa aaaatccttc taaaaatggg
    52081 aacggtaatg gcaaaatatt taaaaccaaa aacatcgagc ctacaatcgt gaaaggaata
    52141 gaagccatcc ccgctgccat gacagcccga acgattttaa gttgcgcaat tttccccata
    52201 ggtcccatca ggtatttctg caaaaaatca aacattagtt attctccttt cctaaatata
    52261 acgctttttc atattgttta atacggatat actgttggtc ttggttgagt gaaattttct
    52321 ttaaacgata ctgtataaat gcactggcaa aaataccgct tacaacaaga atgaaaacaa
    52381 ataactgtcc tccattgtta tttgccataa aaggatacaa gaatgtaaat cctgaggtaa
    52441 acaacactgg aatcactaat aaattaaaga cactctggaa caaaaaatac cactttgtcc
    52501 atggaacgtt cgtttggtgt tttcgataaa gctttacttg ttctcctact gcaggcaaaa
    52561 tagctaaaat cagaatcatc ggtaaactac ttcctaaact tttagctgat agaagcagaa
    52621 tcatccattg catgttcatg aaaaagaaaa aggcagtcag gtaacgtacg attagataac
    52681 gattgaaata catgtttttc aaactaagtt gctttttttc taattcgatt ttccttttta
    52741 ctgtatcctg cacaatgatc cttcctttct atttattttt ttgatacaaa taattcattt
    52801 cctgtgcaac ttccaataat gtcatcgtcg tcatcagatg atcttgtgca tggaccatga
    52861 taatttccat ttctattttt gtcccgctgg catattcttt cagaagttga gtttgtgact
    52921 gatgcgcatg aagaatctct tcatttgctt gtcgcaactt ctcttctact tcttcaaaca
    52981 tctctttacg catcgagcga aatgcttcat gtatcagtgt ccgtgcattt ccgctatgga
    53041 aaatgatatc aaaagaagtc acttgtagtg tttcactgtt tattttttca gccatctgtt
    53101 ttcctcctta ttcgatcagt ttgataaatt tactgctgaa attctcaaaa ataggttcag
    53161 ctaagatttc ctgctgtact tttagctggt caactaacct aacgattgct ttagtcacga
    53221 ccgtaattcc ttcattttcg acataagaag gcgaaactaa aaaaacaaaa tgaatttctt
    53281 gatattgatc ccactgcatc ccagaaggaa tcaatgcaac ggctaccttt gtttcgattc
    53341 caactggaac agcgggatgt gggactgcca ctgtatcact gaatatgatt tgtcccatat
    53401 tttcacgttg ctggatctgc tttcgcattt ctcttatata tccttcttct tcatttatgc
    53461 tcagcttttg cagcaaatca tgaagaattt cttccttttt cggttcgtac gtataagtct
    53521 tgaaaaattt ttcagacaac tgttcggtgt aaatctgctt tttttgctgt agagaaaatg
    53581 ctggtatcgg tctatctgag gaatgatagg gtatagattc tccaactacc tttcgtattt
    53641 tacgaacatc atcttcattc aaaaaaatac tgacatgtaa aactggaatt ttgaaaaaca
    53701 ttgtcgaaag atcaatagaa gaaataatca ggtcgattcc ttttaaagtt gtttcattga
    53761 tctcataata gccttttaca cttaccactg taatattttt accgaattca ctgactacgc
    53821 gattctttaa tagttgggca ctgccgtaac ccgttgcaca gataatcaat gcatgtagtt
    53881 tatgcgcagc tctttctttt tccaatgccg ccatgagatg caaagccaaa taggcccatt
    53941 catcttcatt gatggtatag tttttcaaag ccggcatgtt gctgaaatgc ctttttactg
    54001 cgtcaaagac tttgggattt tctttcttta tctcctcagt caatgggttc tcaatgcgaa
    54061 tacctcggct caatcgaatc atcatcggtg acaaatgatc gagtagtcca ttgaaaagct
    54121 ggagatcctc tactaatgaa aaatgaaatt cctgagacaa tcttttcaga actttcgcta
    54181 attcctttga tagttcctga tcattttgat tttctttgtg attcgactta gccatgagat
    54241 gtaatgtcaa ataagcaatt tcttcttcgg gaaacgatac acctgcgatt acctcaatac
    54301 gttgaacgat ccgctgcgcc acttgatact ctactcgctt agaaacttct tgttcgatgc
    54361 ctaattcctt gatttcaaac ccttctcgga gccgtttgat ccccaaagcc aagtgtaaag
    54421 tcagattctg tatgataaaa tccgaaactt ttaacttagc ttctcttgtt tcatcgagaa
    54481 tgataatgac tagctcttca aaactgatgt ctgggaaaaa acggctttgc cctaaatatt
    54541 ctttcaatga atttccataa tggtttccaa aaaaagtatc catgataaaa tgccgtcgat
    54601 ttctttcttc cccttcgatc catgtccctt gaccatgctt agaaacgatt tttagtgaat
    54661 atggaatcaa tcgctccttg atttcttgca tgtcctttgt caaactggaa cgactgatat
    54721 aaagctcttc tgccaaatca tccataaaaa gaatggcatc ttccaaaagc aattggttca
    54781 atatatactt ttgtcgatct tcggactcat aaaatccttg cggttctcta gcatatccag
    54841 gtactatttc ttgccgactc aaaaacaagt caaatgtcag tctttgtaaa atatgcagtt
    54901 ggtagccata acctggttta gcaatgatct tacctccgtt tttttctact aactctttga
    54961 tccgctgcaa gtaattacgg attgtccgat cagataataa caattcagaa gctaaacgtt
    55021 gactggtgac atactccttc ttgtgatcaa tcaaatatct caacagcaat ttttccttct
    55081 gtttcattat catccactcc tattccatca tatgttctct tcgatcacct ctgctaactt
    55141 ctggatgcct gtagggattg ggacatacgc ttggaaaggg atactaacaa caggctttcc
    55201 aacagattct cctaattttt tgaatttatc caaaaacatc gtcgtttgcg ggctgatcag
    55261 aaataaatca aaatcactat tcttaatcat attatcgcct tctgtagctg aaactgcatc
    55321 tacgagtacc tcttctcttt tttgcttaaa gaaatccgtt gtctttgagg ccatcatcga
    55381 ggatgacatt cctgccgcac aaataatcaa tgcttttttc ataaatcaat cactcctttt
    55441 atttttataa accaagcata tcttgtaagc gttttctatt caaaatatat atttccgtag
    55501 cattgcggaa aaaatgtgta tcatatcttg aataataatt tctggcaccg tgatatacta
    55561 gtgatagaat tttttcagga ggggatttag tgagtatcac agtgaatatt ttgtatacaa
    55621 gtacaaatgg acaagccaga aaatttgcag aagaaatggt cgaaagaaac attgtcaaag
    55681 cgattcgtgc agaagaagga aacgagaaat atgactatta ctttccacta gaagatccag
    55741 aatctctttt actgatcgac cgttggaaag atgaagaagc aatcgaaaag catcacaaat
    55801 ccgaaatgat ggctcaaata aacgaactcc gtaaaaagta taaattaaaa atgaaagtcg
    55861 aacgtttcac tgatttaccg taaaaagata cacatggaat gatagcaatt tatcatccat
    55921 gtgtatcttc actttttcct tattttttat tcgatctcat ctaatgattc aaaccgtgtt
    55981 tttctcttat ttttcggata agtatttcag tcaccccaat atacatgatt cctaaacata
    56041 ccccaaggat attctcaaaa atgcggatcg ttactgctcc tggaaccccg aataaactgg
    56101 cagtgattgc caaagcacca aaagaattaa aaactgtttt atatctatag gtagaacaaa
    56161 cacctaaagc tagaccacct aatataccga cccagttaaa aggaataaat tggctgatac
    56221 caataaataa aacacatcca atcaatgagc caatgattcg gtctactgct cgttctttga
    56281 aaccaatcga catcaatcca tagctagata caatagaaga aaatgcaaac cctgcccaca
    56341 tcaatcgctg aaaagggaca tactctcctg caacgatcaa aagactaatg ccaaatgcat
    56401 agatgagcat ccaaattttt tcctgcgaat acatgccttt caaaaaaatc tctttaaaca
    56461 agcttttgtc tttgttcttt tctcgatgtt ttctgtaaag gagaactgaa aaccaacaaa
    56521 aaaacaaaaa taataagcta cttgtttgtg taaagaaatt cttgttcaac gaatctttag
    56581 gtaacgaata aacaataaag agatatgaaa agccgtacaa agaagcattc cccattttgg
    56641 gatcactagc ggttgccatt aatatgctgc ttagtaaaat aaaatgaatc aacatgccca
    56701 taactgacca tttgatcagc tggacatacg gtgctactaa tagtgagaac atgacgattg
    56761 ctaaacctat gatcgaatgg gaaatcttat aaccaaagtc aacaaaacga atactcatca
    56821 acatacagaa taaaaccaca gcgtatggtt tatgcttcga ttcaaataat gttgtaatta
    56881 taatgataaa acaaatggca aacaatacga tgaataatga tcgtaaaaac aatgaacaaa
    56941 tatacatgca cttttctttt ttagaagctg cttctcgtat tttttgtttt aaaataaacg
    57001 gatctagttg tagagactga taaaaatcca cctgttgtcg tcctttcttt ttacttttat
    57061 tttttacagt catctatgac aggttgtaaa tactatggaa catcatatgg cgttgtcatt
    57121 ttcttgtcaa ctatttttcc tattcatgtt tttataaaaa aacatatcgt tttcttgtat
    57181 gattaaatca ccacaaaata aacaactgag agaacgatat gctttatgaa tggttctatc
    57241 aaaaaaattc tgagatcaat agacaaaaat ttgatgatta cagaggtctc ttacgaaacc
    57301 ttacagaaga aaaaaacatt gatcgttgct gcagttctct tccctgctcc atgtgtctgt
    57361 agaaattgtg gttctgctgt ggtagatgga aataggaaat taatggtggt gaaaaacaag
    57421 aagaaggaaa ccattgtcca ctttgaataa tataatcata tgccaatggt tatacgccta
    57481 aaaaagcagc gctatacctg taaaaactgt cgaagccatt ggaacgctca aagttatttt
    57541 atccgtccaa ggcactcgat ttcaaatcat gtcagacata aaatcacttc tttactgact
    57601 gaaaaagtgt cgttattttt tatttcgaaa agttgttagg tatctttgac aaccgttatt
    57661 cgcacattga aagagttgag aagctattta ccaaagtctt ccaaaagaat tctgtcttga
    57721 gtgttgaggg ttgtgaattt cgttctcacg tttccgttga ggataaactg agttttattt
    57781 ccgcggatgg cgagacagaa aaattaatcg agaaaattta cctacacaaa aaattcctcg
    57841 attaacaaac tatttcctta gctgcactaa tcctaaagaa atagaattct tagttacaga
    57901 catgaacgcc acctactttc agctcaccaa acatgttcta ccaaatgcga aattggtgat
    57961 tgatcgatta cttggttttt ccacgccttt caaggaggcg taacctttat tcacgagttg
    58021 gttgaagctt tccgagacaa aagacccgac ttgtttttct ccttattgac aaagagccat
    58081 taaaactaga gaaaaaatta caactgatcg ctgattattt gtagaaagaa aatcaaagta
    58141 ttacccaata aacttgagtc attaggcaaa aaacactgcg aaactcaaga atgattttcg
    58201 cagtgttttt gtatattttt tattaactag tattactaca aaggtttatt tttcacttct
    58261 caacgcagca caaaattgtc tttacttatt tactggttgg gttcaacctt ctctactccg
    58321 atactaatat ttgtttgtat accccttcta aaatcggtag tttccattca attgatgggt
    58381 actagttgat tttaaaaact atgcttattc aattttttat tttagctcat atatttcgtt
    58441 tctttctaaa ttttatactg atgaaaacaa gtagcacacc caatattgac aatataaact
    58501 ggctaatgat ggtttctcca gtttttggaa gatgcttatt tccactgctt gttggattgt
    58561 ttcctcttat atctgagcta tcagcaactt ttctattctc ttttgttaaa aagatcgcca
    58621 cttcttgttc tttttcgatc ttgaaagtta ctggtgtcgc atccaattca taccccgttg
    58681 gcgcttgagt ttctaccagc tgatatgctc ctggtgctaa gccatctatc gccagttttc
    58741 catctgctcc tgtggttaga cctgactgca gaacggttcc attggcatct tgtaactcaa
    58801 aaacagctcc ttgcaggact tctccgcttt ggtcatcgat ctttgtcaac acgacaccgc
    58861 ctggtgtaag tcgattttct ttcgtcaatt ctaccaccgc tgtctgactt ctttcgatct
    58921 caaactcgat tggtgtcgca tccaattcat accccgttgg cgcttgggtt tctaccagct
    58981 gatacactcc tggtgctaag ccgtcaatcg ccagttttcc atctgctcct gtggttagac
    59041 ctgactgcag aacggttcca ttggcatctt gtaactcaaa aacagctcct tgcaggactt
    59101 ccccgctttg gtcatcgatc tttgtcaaca cgacaccgcc tggtgtaagt cggtttgctt
    59161 tcgtcaattc taccaccgct gtctgacttc tttcgatctc aaactcgatc ggtgtcgcat
    59221 ctaattcata cccc
//
LOCUS       DACEQH010000010        53700 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.010, whole genome shotgun sequence.
ACCESSION   DACEQH010000010 DACEQH010000000
VERSION     DACEQH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.010"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            83..970
                     /locus_tag="IW833_000618"
     CDS             83..970
                     /locus_tag="IW833_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1306756.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="IW833_000619"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="IW833_000619"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ1306757.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="IW833_000620"
     CDS             2272..2718
                     /locus_tag="IW833_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1306758.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="IW833_000621"
     CDS             2991..4343
                     /locus_tag="IW833_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ1306759.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="IW833_000622"
     CDS             4514..4801
                     /locus_tag="IW833_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306760.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5027..5470
                     /locus_tag="IW833_000623"
     CDS             5027..5470
                     /locus_tag="IW833_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1306761.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5476..6660)
                     /locus_tag="IW833_000624"
     CDS             complement(5476..6660)
                     /locus_tag="IW833_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ1306762.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6928..7626
                     /locus_tag="IW833_000625"
     CDS             6928..7626
                     /locus_tag="IW833_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ1306763.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7633..8310
                     /gene="nth"
                     /locus_tag="IW833_000626"
     CDS             7633..8310
                     /gene="nth"
                     /locus_tag="IW833_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ1306764.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8436..8969)
                     /locus_tag="IW833_000627"
     CDS             complement(8436..8969)
                     /locus_tag="IW833_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306765.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9266..11647)
                     /locus_tag="IW833_000628"
     CDS             complement(9266..11647)
                     /locus_tag="IW833_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1306766.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IW833_000629"
     CDS             complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IW833_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ1306767.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12348..12887
                     /locus_tag="IW833_000630"
     CDS             12348..12887
                     /locus_tag="IW833_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ1306768.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12977..13396
                     /gene="gpsB"
                     /locus_tag="IW833_000631"
     CDS             12977..13396
                     /gene="gpsB"
                     /locus_tag="IW833_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ1306769.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13418..13785
                     /gene="rnpB"
                     /locus_tag="IW833_000632"
     ncRNA           13418..13785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IW833_000632"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13994..15160
                     /locus_tag="IW833_000633"
     CDS             13994..15160
                     /locus_tag="IW833_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ1306770.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15206..16702
                     /locus_tag="IW833_000634"
     CDS             15206..16702
                     /locus_tag="IW833_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ1306771.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16783..17466
                     /locus_tag="IW833_000635"
     CDS             16783..17466
                     /locus_tag="IW833_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1306772.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18111..19301
                     /locus_tag="IW833_000636"
     CDS             18111..19301
                     /locus_tag="IW833_000636"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ1306773.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19403..20356
                     /locus_tag="IW833_000637"
     CDS             19403..20356
                     /locus_tag="IW833_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ1306774.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20425..21774
                     /locus_tag="IW833_000638"
     CDS             20425..21774
                     /locus_tag="IW833_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1306775.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21957..22280)
                     /locus_tag="IW833_000639"
     CDS             complement(21957..22280)
                     /locus_tag="IW833_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306776.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22409..23572
                     /locus_tag="IW833_000640"
     CDS             22409..23572
                     /locus_tag="IW833_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306777.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23642..24991
                     /locus_tag="IW833_000641"
     CDS             23642..24991
                     /locus_tag="IW833_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1306778.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25036..25467)
                     /locus_tag="IW833_000642"
     CDS             complement(25036..25467)
                     /locus_tag="IW833_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1306779.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25855..28497
                     /gene="alaS"
                     /locus_tag="IW833_000643"
     CDS             25855..28497
                     /gene="alaS"
                     /locus_tag="IW833_000643"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ1306780.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28697..29293
                     /locus_tag="IW833_000644"
     CDS             28697..29293
                     /locus_tag="IW833_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1306781.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29415..30563
                     /locus_tag="IW833_000645"
     CDS             29415..30563
                     /locus_tag="IW833_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1306782.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30601..31014)
                     /locus_tag="IW833_000646"
     CDS             complement(30601..31014)
                     /locus_tag="IW833_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ1306783.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31093..31524
                     /locus_tag="IW833_000647"
     CDS             31093..31524
                     /locus_tag="IW833_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ1306784.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31623..31856)
                     /locus_tag="IW833_000648"
     CDS             complement(31623..31856)
                     /locus_tag="IW833_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ1306785.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32039..33709
                     /locus_tag="IW833_000649"
     CDS             32039..33709
                     /locus_tag="IW833_000649"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ1306786.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33856..34242
                     /locus_tag="IW833_000650"
     CDS             33856..34242
                     /locus_tag="IW833_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306787.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34229..34927
                     /locus_tag="IW833_000651"
     CDS             34229..34927
                     /locus_tag="IW833_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1306788.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34932..35396
                     /locus_tag="IW833_000652"
     CDS             34932..35396
                     /locus_tag="IW833_000652"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ1306789.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35393..36304
                     /locus_tag="IW833_000653"
     CDS             35393..36304
                     /locus_tag="IW833_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1306790.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36539..37078
                     /gene="pyrR"
                     /locus_tag="IW833_000654"
     CDS             36539..37078
                     /gene="pyrR"
                     /locus_tag="IW833_000654"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ1306791.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37092..38375
                     /locus_tag="IW833_000655"
     CDS             37092..38375
                     /locus_tag="IW833_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ1306792.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38388..39314
                     /locus_tag="IW833_000656"
     CDS             38388..39314
                     /locus_tag="IW833_000656"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ1306793.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39333..40613
                     /locus_tag="IW833_000657"
     CDS             39333..40613
                     /locus_tag="IW833_000657"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ1306794.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40618..41694
                     /gene="carA"
                     /locus_tag="IW833_000658"
     CDS             40618..41694
                     /gene="carA"
                     /locus_tag="IW833_000658"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ1306795.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41699..44884
                     /gene="carB"
                     /locus_tag="IW833_000659"
     CDS             41699..44884
                     /gene="carB"
                     /locus_tag="IW833_000659"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ1306796.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44938..45714
                     /locus_tag="IW833_000660"
     CDS             44938..45714
                     /locus_tag="IW833_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ1306797.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45711..46643
                     /locus_tag="IW833_000661"
     CDS             45711..46643
                     /locus_tag="IW833_000661"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ1306798.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46640..47356
                     /gene="pyrF"
                     /locus_tag="IW833_000662"
     CDS             46640..47356
                     /gene="pyrF"
                     /locus_tag="IW833_000662"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ1306799.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47363..47992
                     /locus_tag="IW833_000663"
     CDS             47363..47992
                     /locus_tag="IW833_000663"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ1306800.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47980..48294
                     /locus_tag="IW833_000664"
     CDS             47980..48294
                     /locus_tag="IW833_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306801.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48332..48973
                     /locus_tag="IW833_000665"
     CDS             48332..48973
                     /locus_tag="IW833_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1306802.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49004..49708
                     /locus_tag="IW833_000666"
     CDS             49004..49708
                     /locus_tag="IW833_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ1306803.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            49705..50607
                     /locus_tag="IW833_000667"
     CDS             49705..50607
                     /locus_tag="IW833_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1306804.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50765..52471)
                     /locus_tag="IW833_000668"
     CDS             complement(50765..52471)
                     /locus_tag="IW833_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ1306805.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52661..53284
                     /locus_tag="IW833_000669"
     CDS             52661..53284
                     /locus_tag="IW833_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306806.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
BASE COUNT        17594 a         9322 c        11518 g        15266 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaat cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa
     4981 taggaaacaa aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca
     5041 actgctaata gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga
     5101 aatggaaaaa gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg
     5161 gaaaatccct gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc
     5221 catcagtaag ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga
     5281 agagaataaa aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag
     5341 acacgagcaa ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc
     5401 aaaagaagaa tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag
     5461 tagcaaatga agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa
     5521 tccgaatacc gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc
     5581 gatgacgcgc gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt
     5641 atctgagaca gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa
     5701 accataacag ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt
     5761 gatgaataga taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga
     5821 aaattttttg agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat
     5881 caaagagaag cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg
     5941 agaaaagata gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa
     6001 aggaataacg gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga
     6061 gaaacttaaa tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc
     6121 ataaacggca tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa
     6181 ctcagctaat agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc
     6241 catccatcca aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata
     6301 taaggtggag atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca
     6361 tatcgaattt cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat
     6421 aaggatcgga tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa
     6481 ggcagctaga gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga
     6541 gtgagaataa ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc
     6601 agtgaataag acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat
     6661 gtaaaaactc ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt
     6721 gtttccttgt caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag
     6781 tgaagatacg catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg
     6841 accgatcagt gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt
     6901 atactgtgat tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga
     6961 caaacagtca tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa
     7021 gaatttttat ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat
     7081 ttgcagatga ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat
     7141 cgtctcgtaa caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca
     7201 gatcgatata atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa
     7261 aaaaaatacg aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa
     7321 caagaatttg gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa
     7381 gaagaccatt atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag
     7441 gcttatagtt ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt
     7501 acaaaacaac aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa
     7561 gtagaacagt cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag
     7621 gagtagcgga atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga
     7681 atgtttcccg aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca
     7741 gtgatcttaa gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt
     7801 gcctcttttc cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa
     7861 ataaaaacga ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag
     7921 ctgattgaac gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct
     7981 ggtgtaggca gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc
     8041 gcagtagata cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct
     8101 acggtcatgg aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag
     8161 acacatcata cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc
     8221 gaggtatgtc ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa
     8281 aaggcgctaa aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta
     8341 tgaattatct tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt
     8401 aaatgtacat actcagtagc taggatggta acttattaca atttattgcg ataaaaataa
     8461 aagttgttat ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct
     8521 ataaaataag tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt
     8581 gtaataaaaa tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg
     8641 gatttgggcg gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa
     8701 aaagtgagaa tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt
     8761 aaaagtacag aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat
     8821 aataaacaaa atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa
     8881 aaaagatggt gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag
     8941 ccttctttca aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg
     9001 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
     9061 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
     9121 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
     9181 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
     9241 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
     9301 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
     9361 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
     9421 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
     9481 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
     9541 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
     9601 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
     9661 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
     9721 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
     9781 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
     9841 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
     9901 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
     9961 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
    10021 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
    10081 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
    10141 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
    10201 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
    10261 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
    10321 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
    10381 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
    10441 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
    10501 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
    10561 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
    10621 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
    10681 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
    10741 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
    10801 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
    10861 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
    10921 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
    10981 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
    11041 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
    11101 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
    11161 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
    11221 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
    11281 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
    11341 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
    11401 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
    11461 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
    11521 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
    11581 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
    11641 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
    11701 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
    11761 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
    11821 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
    11881 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
    11941 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
    12001 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
    12061 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
    12121 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
    12181 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
    12241 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
    12301 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
    12361 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
    12421 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
    12481 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
    12541 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
    12601 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
    12661 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
    12721 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
    12781 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
    12841 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
    12901 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
    12961 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
    13021 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
    13081 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
    13141 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
    13201 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
    13261 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
    13321 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
    13381 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
    13441 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
    13501 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
    13561 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
    13621 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
    13681 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
    13741 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
    13801 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
    13861 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
    13921 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
    13981 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
    14041 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
    14101 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
    14161 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
    14221 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
    14281 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
    14341 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
    14401 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
    14461 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
    14521 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
    14581 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
    14641 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
    14701 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
    14761 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
    14821 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
    14881 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
    14941 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
    15001 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
    15061 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg
    15121 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
    15181 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
    15241 aatcaatctt ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
    15301 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
    15361 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
    15421 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
    15481 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
    15541 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
    15601 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
    15661 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
    15721 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
    15781 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
    15841 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
    15901 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
    15961 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
    16021 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
    16081 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
    16141 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
    16201 ttatcgttca ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
    16261 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
    16321 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
    16381 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
    16441 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
    16501 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
    16561 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
    16621 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
    16681 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
    16741 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
    16801 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
    16861 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
    16921 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
    16981 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
    17041 cagatctcga tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa
    17101 gcggccgaac tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta
    17161 ggagcaatcg gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt
    17221 gatcctgaac tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca
    17281 gtgatcaatc atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac
    17341 ggatatgaag aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt
    17401 ctggaatggt ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa
    17461 aaatgatgga ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta
    17521 aaagttactt ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt
    17581 cacaaggaac aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt
    17641 cggaattggc aggagaacac cgtttattcg gaagaatagg actgtgacaa gacttttgtc
    17701 acagtccttt tttcgaattc aacaagtaaa acttttaaga aatattcaga gagtaaaatt
    17761 aaaaattgct gataaacgaa gatggaaatt ttatgtattt ttattggtct atgcaataaa
    17821 taaattaaat attcagaata caattgattt taacgaaagg gcttctatca atggttgtgt
    17881 tgtatttatc tgcccaggta gagtaaaata gatcattatc taaaaggtaa ttgttcatac
    17941 tatataagtc atttgaaagt aaaaagatac tgtaaataaa aaatgtggtt aaataattct
    18001 cataatcagt gtaagcgtta tcaaaaatat agcatacttg ctttgtagat gaatgacacg
    18061 atatatgttt ggtatgttct gcaagacaga tgaatatgga ggtggagaca atgtcttcca
    18121 aagaactaac aatgtattta gagaaagaat tggatcaact gaaagaaaag gggttataca
    18181 atacgatcga catactagaa agtgaaaatg gtgcttgtat catcgtagac ggaaaaaaga
    18241 tgatcaatct agcatccaac aattatcttg gatttgccaa tcgcgaagaa ttgaaaaaag
    18301 catgtattga agcaaccgaa acttatggtg taggagcagg agcagtccga acgatcaatg
    18361 gttcgctgaa aatccatcag caattagaag aaaaaatcgc agaattcaaa gggacagaag
    18421 cagcaatcgc ctttcaatct ggattcaact gcaatatggg cgctatctct gcagtaatga
    18481 caaaagaaga tgctatctta tcggatgaat taaaccatgc atctattatt gacggttgcc
    18541 gcttatccgg agcaaaaatc atccgaatta aacatcaaga tatgaaagac cttgagaaaa
    18601 aagcaaagga agcgatagaa agtaagaaat ataaaaaaat catgtatatc acagatggtg
    18661 ttttctccat ggatggagac attgcacgat tgccggaaat tattccgatt gttgaaaaat
    18721 atggactgat cacctacgtc gatgatgcgc acggctcagg cgtaactgga aaaggtgcag
    18781 gaaccgtcaa gcattttgga ttaagcgaca aaatcgatat gcaaatgggc acgttatcga
    18841 aagctatagg tgttgttggg ggttatgtag ctggatcgaa aacactaatc gactggttga
    18901 aagctagaag tcggccattc ctattttcta cttcgctaac tccaggtgca gctgcagctg
    18961 cattggcttc gattactttg atgcaagaac atccggaact tgtcgaaaaa gtttgggaaa
    19021 atgctaatta ttttaaagaa gaattgaaaa aagtaggcta taacatcggt atgtctgaaa
    19081 cacctattac tccagtcatt ttgggagacg aaaaagtgac tcagactttc tctaagaagc
    19141 tgatcgaaca tgggatttat gctaaaccaa tcgtttatcc aactgtgcct cttgggacag
    19201 gcaggatcag aaatatgccg actgcggaac atacgaggga aatgcttgat gaagcagtag
    19261 cagtttatca aaaaatagga aaagaaatgg aaattattta actaaattta gttttttcgt
    19321 ctatttaaaa ttagagaaca tgttcacgca tatattgtta atcatctgaa gatagtagta
    19381 aaaaagatca gggaggatca tcatgaaaaa agtattggtg acaggatgtt taggacaaat
    19441 tggttcagag ttgatcacac ggctacgtca agaactaggc agtgaaaatg tcattgcgac
    19501 agatatacgc atgcctgaag tcattgagaa aaacgatatc tttgagatcc tagatgtaat
    19561 ggattacgaa aaaatgcgca gcatagcaga acaatatcaa gtggacacat tgattcattt
    19621 ggctgctctt ttgtcagctg ttgcagaagc aaaaccacaa ttagcctgga atttgaacgt
    19681 gactgggctg gtcaatgcat tggaagtcgc tagagaattc cagctgaaat tttttacgcc
    19741 aagttcaatc ggcgcgtttg gtccaaatac tccaaaagat cataccccac aagatactct
    19801 tcagcgtcct acaaccatgt atggggtaac gaaggtagct ggagaactac tatgtgatta
    19861 ttattatgtg aaatatggcg tagatactcg tggtgtccgt ttccctggat tgatttctta
    19921 tgcaaccttg ccaggaggcg gtacgacaga ctacgcagtc gatatttatt attcagcaat
    19981 caaagaagga aagtacacta gtttcattga tcaaggtaca tcaatggata tgatgtatat
    20041 gccagatgcg atcgatgcca ttataaaact catgaatgca gatcctgatc gattggttca
    20101 tcgaaatgcc ttcaatatat cggcgatgtc ttttgacccg gaagagatca aagcatctat
    20161 tcaaaaaatc ataccagatt ttgaaatgga ttatgatgtg gatcctgtac gtcagtcaat
    20221 agcagaatct tggccaaatt cactagatgt ttcttgtgcg caagaggaat ggggcttttc
    20281 gccgaaatac gacttagatg tgatgacaaa ggatatgttg gaaaaactga aagaaaaatt
    20341 ggtagagacg gtataaaaca agaaccacat ccaaaaagtt ttttatactt ttgggattgg
    20401 ctatggtatg aaaggggaca agggatggaa gaatatcaag ccacgccagt aaaagaagta
    20461 aaaatagaaa atgaattaaa acgtacgatg ggatttttta ctgctttatc gactgttatg
    20521 ggcacagtga tcggtgcagg ggtatttttt aaagccgcaa gcgtagcaga agttacagga
    20581 tccgccagtt tgcatatgtt ttcttggttt ctcggtggta tgatttctgt ctgtgcagga
    20641 ctaacaggag ctgaactggc agctgctatt ccagaaacag gagggatgat taaatatatc
    20701 gaacggattt acggaaatac agcagctttt ttattgggct gggcgcaagt cgtgatttat
    20761 tttccagcaa atgtagcagc cttgtcgatc attttcggta cacagttcgt caatctattt
    20821 ggtctttccc agtcaatgat tgttcccgta gctgttacag ctgctgtatc gatcctgttg
    20881 atcaattttt taggttctaa agctggaggg gcttttcagt cgattacgct tgtctgcaag
    20941 cttattcctt tgtttgtgat cgtcattttc ggtttgttcc gacaagaagg agtagacttc
    21001 caactgtttc cgatccaagc aggagaaaat ctttcatttt tctcagcctt gggagcgggc
    21061 ttgctagcaa cgatgttcgc atacgacggt tggatacacg taggaaatat ttcgggagaa
    21121 ttgaaaaagc cggcgaaaga tttgccaaaa gcaatttctt taggaatcat cggaatcatg
    21181 atcgtttatt tattagtaaa tgcggtgttc ttaagaaccg catctatcga cggagtcgca
    21241 ggaaacagca atgcggcaag tgacgttgca aaaatgattt ttggcggctt cggtggaaga
    21301 ctggtcacag ttggtatctt gatttctgtt tatggaacca tcaatggcta tacccttact
    21361 ggaatgcgtc tcccttatgt tatggcaaaa gaaaacaacc tgccttttag caagctcttt
    21421 gctaaacttc atgataaaac taaagttcct gtagccgcag gtatcttaga gctagtcatc
    21481 gctattggca tgatgatgat cggtggtttt gatacactaa cggatatgct gatctttgtc
    21541 atttggattt tttatacgat ggtctttgtt ggtgtcattc ttcttcgtaa aaaagaacct
    21601 gatttgtttc gaccatacaa agttccaatg tatccgttta ttccactagt agcaatcatt
    21661 ggagggactt ttatcgtatc aagcacgctt atcactcaga cctttttagc ttcgatgggg
    21721 atagtattga cacttgctgg tatcccgatt tatctctatt taaaacgtag atagctaaaa
    21781 cattcgaatt ttattagaac tgcacttctc tgccaaacaa aatattttat ttaaatgagg
    21841 agtacgtgga agaaatgtca aaagtactcg ttaattttga tcacgtaaat gtcgttttgt
    21901 ctacgatgaa atagcaaagt aaaagacaga aatcgggctc aaccgatttc tgtcttttac
    21961 tttgatttgc gtaactgaac tattcggcgt acgccgcgat aaatatttaa taaaaccaag
    22021 caaccagaaa tgaaaatggg aagccaaatc agatagacag ggtagccaag cactttagac
    22081 aaagcatgtg gagtcatttt atccatttca agaagtgctt gctgccaagt ctgaagattg
    22141 aagactagca tgaaacccga aataatcagg agaataaatc ctaaataatg aattgtagtt
    22201 ctttttctta cataagcgat cgtagcataa aggaacagta aaaaaatagg tacgccaaaa
    22261 acaaagtaca tattcagcat ctcgacaccc cctttgtgtt tatcttactt tactttgaaa
    22321 gaaatgagaa ataatagcct cgttacttgc ttgaaacagc aaaggactgc taagataaga
    22381 gagatacaaa gaatcaagga ggagtcatat ggcaagttat atcgaaccga ttcgttttgc
    22441 gctgattgtt tttccatttt tagccttagc gatctcatct gtttttttta tttatgaata
    22501 tcgaaagtat ggaacatttc tagtaacaag ggcagttatt ttgtattctt tcgtttttta
    22561 tttattgtgt gcgtattttt tggtgatctt accacttccg gcaaaggaga cggttgccca
    22621 aatgacaggg ccaaagatgg agctgcattt atttgcttca tggcaacatt tcagagacaa
    22681 cacggtattg gtcttgtctg aacctagtac ctacctgccg gcaatgaagc agagtgtatt
    22741 tttggaacca gcttttaatg ttttattact cataccattt ggcatttatt taagatatta
    22801 tttccggctt tcttttatga aaacgatgat tgctagtttc tgcctttctc tattttttga
    22861 attgacgcag ctaagtggac tttattttat ctacccaaga ccataccgct tatttgatgt
    22921 gaatgattta gtgacaaata cattgggtgg aatcattggt tttgtcgtta cgccgttatt
    22981 tacatttatg cttcctacaa gaagacgaat ggatgaactt tcttatgaaa aaggaaagac
    23041 tgtctcattg actcgacgct tggtcgctta tttgatcgac tggacatttt tatctcttat
    23101 taatttggta gcggcgattt tctttaaaat tgcgttcgat tcaggggcag ttacttctca
    23161 tatctggtgg cttttattag aagtaggggt atactttatg attgtaccgt ttataaccaa
    23221 tggatggaca cttggcaaaa aaattgtgcg tatccgtttg gtggaagaag accgcgaacg
    23281 aatttcttta aaagcattga tgatccgcta cgggtatctg tactttcttt tttatggttt
    23341 aagctggttg gggcagttgc ttcagcaaat catgaattcg acaacgaata cggtgttgca
    23401 gatgagtgca gtattcattt ctctcggtgt cttgttacta caaggtattt ttattttaaa
    23461 tatcttgcta tcatttttga gaaaaaaacg gattctgttt tatgaaaaag ctagtcacac
    23521 atatactgtt agcacgattg gccaaacaaa ggatgatgat tctcgaaatt agctgagcaa
    23581 aaacgctttg tctgtgttat aatgccaaag tcgtgataat ttaaagagaa gaggtgcacg
    23641 aatgaataat tttgatatat tccgcttcaa accgtttatc aatgaagcgt taaccgcaaa
    23701 gggcttcaaa cagcccacag aggtccagga acgtttgatt cctttaattg caaaaggaaa
    23761 aaatatcatt gggcaatctc aaactggtac aggaaaaaca catacgtttt tgttgccttt
    23821 gtttgaaaaa atcaatgcag aaaaagagga agtacaagtt gttattacag ctccaagtag
    23881 ggaactagct gcacagatct atgaagcagc tacacaaatc gctaaatttt ctacaccgga
    23941 gatccgtgtc tcgaattttg tcggcggtac agacaaacag cgtcaattaa atcgtctgca
    24001 tcaccaacag ccacaggttg tagtaggcac gcctggtaga atattagaca tgatgaatga
    24061 acaagcgctg aagattcaca ccgcatttgc atttgttgtc gatgaagcgg atatgacatt
    24121 ggatatgggt tttttaaatg aagtagacca aattgcaagt cgattaccag aaaagctgca
    24181 gtttttagtt ttttctgcca ctattccaga aaaacttcgt ccattcttac gaaaatacat
    24241 ggaaaatcca atcattgaag aaatcaaacc aaaagcagtt atttctgaag acattgaaaa
    24301 ttggctgatt tcaacaaaag gtaaagatga gaatcaaatg atttatcaac tgctgacaat
    24361 tggtcatcct tatttagcaa tcgtatttgc caatacaaaa cagcatgtgg atgaaattgc
    24421 cgattacttg aaaaaccaag gcttgaaagt agcaaagatt catggagaca tcaccccaag
    24481 agagagaaaa cgagtgatgc gacaagtaca gaatctggag tatcaatatg ttgtagcgac
    24541 tgatttagct gctagaggga tcgatatcga aggtgtctct catgttatta atgcagaaat
    24601 tccagaagat ctagacttct ttatccatcg tgtcggacgt actggacgta atggcttaaa
    24661 aggaacggcc attacgttat attcgccaaa tgatgagcaa gccattgatg cgatagaaaa
    24721 attgggcgtc tcatttgaac caaaagaaat caaaaacggg gaaatcgtca aaacatacga
    24781 tcgtaataga cgtgccaaac gagaaaaatc aaaagatgta ttagatccca cgctgattgg
    24841 tcttgtgaag aagaaaaaga aaaagatcaa gcctggttat aagaaaaaaa tcgattgggc
    24901 aattgcagaa aaaaataaaa aagaacgcaa aatcgaacgc agacagcaaa gccgtactgc
    24961 tcgtaaaaat aagaaaaatt cacataaata aaaaacaaga ggttgtgaca aaaggcgtgt
    25021 cacaatctct tgtttttaag ctagactttt tttcattccg atacaaagta cttcatcttc
    25081 aataaaaggt tctgtgcaag tatgataccc ttgtttttca taaaaatcta cagccgtcat
    25141 ttcagctact acataactag tctgacaacc ttctgaaacc gcccgttttt cagcagcttc
    25201 aagcaactga ccgcctaacc cttttcctcg gtattctttt gctacgcaaa atcgttccgg
    25261 cttgaagcaa gtaggtgttt tcttttcata acgcaaagta gctactggtc ttttttgatc
    25321 aagaaataga taataggttt gttgcgggat atcttttccg tcgaattcta gttctgcagg
    25381 aatcccttgt tctaatacaa aaacttgata tctcaaaaag cagctagctg cgcgtatcca
    25441 ttcatccgct ccaactattt ttttcatcat attcctcctt gtcatgaata gcagagatgt
    25501 attgattcct ttattcttat cgtaccaaaa atcgtggagt atgaccaaaa ggaaatgaaa
    25561 ataaaaattg agatattgac agtgatcaat agaatgcgta tactaaataa gtacgaaaag
    25621 gatatgattt gttttttaag actcacagaa aattctttct tggctgaaaa aagaatagtc
    25681 actaatcaaa ttgctccctt tttctatttt ggtagaaata ccgacgcagc tcagcgttat
    25741 cgagtttaag aggtaatgat tacacatcat tgcaatcaag gtggtaccgc gagttttcgt
    25801 ccttggctgt gatggtgttt ttttgtattt aatttataga aagcaggtaa tgacatgaaa
    25861 caattaagta gtgcacaagt tcgacaaatg tttcttgatt tcttccaatc taaaggacat
    25921 acgattgaac ctagtgcgtc tttgatcccc gtgaacgatc caaccttatt gtggatcaac
    25981 tctggtgttg caacattgaa aaaatatttt gatggttcag ttgtgccaga aaatccaaga
    26041 atcacaaatg cccaaaaatc tattcgaaca aatgatatcg aaaatgtcgg taaaacagca
    26101 cggcaccata cgatgtttga gatgctgggt aatttttcga ttggtgacta tttcaaaaat
    26161 gaagccatcc actgggcatg ggaatttttg actgatccaa aatggatggc attcgatcca
    26221 gaaaaacttt atgtaacagt ttatccaaaa gatacagaag caaaacgcat ctggcatgaa
    26281 gaagttggcc tgccactaga tcacattatt gatgtagaag acaacttctg ggatatcggt
    26341 gcaggcccat gtggtccgga tacggaaatt ttctatgacc gtggagaagc ttataacgat
    26401 ttagcagaag acgatcctga aaactatcca ggaggagaaa atgaacgtta tttagaaatc
    26461 tggaacttag tattttcaga attcaaccat aagcctgata acacatttga accacttcct
    26521 cataaaaaca ttgatacggg gatgggattg gaacgcatgg tctcaatcgt tcaagatgcg
    26581 tcaacgaact ttgagacaga cttgtttatg cctattatcc acgccgtaga aaagctaagc
    26641 aaacaagtaa aatatggtga aaacccagta acagatattt cttacaaagt aatcgcagat
    26701 catattcgcg ccctttcatt tgctattggc gacggtgcat tgccatcgaa tgaagggaga
    26761 gggtatgtct tacgccgttt gcttcgtcgt gctgtcatgc atgggaaaaa attaggtatt
    26821 gaacaagcat tcttatatcg tttagtccct gttgttgggg aaatcatggt aagttattat
    26881 cctgaagtac ttcaacaaaa agaatttatt gaaaaagtta tccgtacaga agaagaacgt
    26941 ttcaatgaaa caatcaatga aggtttggaa atcttaaatc aagtgatcca agaagtaaaa
    27001 aacgaaaacg ggacgactct agcaggaaaa gacatcttca aactatacga cacatatggc
    27061 ttcccggtag aattaacgga agaggtggca gaagatgaag gactgaaagt tgatcatgct
    27121 ggttttgaaa aagaaatgga tgcacaacgc gaacgtgctc gctctgcccg cagtactgaa
    27181 aaatcaatgg gggtacaatc tgctttattg acagatatca aagtagaaag taaattcatt
    27241 ggatacacag aacaagaatc agatagtcaa ttattggtca tcgttaaagg tgagtctctt
    27301 ttgaatgaag tcgcagaagg tgaagctcaa atgatctttg atcaaacacc tttctatgca
    27361 gaaatgggtg ggcaagtagc ggatcaagga tggatcatga atgaagtagg cgaaatcgta
    27421 gcagttgtta cagatgttca aaaagcgcct aatggtcaat ttatgcacac cgtagaagta
    27481 aaagcaccaa tgagtgaagg acaaacctat catctattga tcgatcaatc gatgcgcaat
    27541 cggatcatca aaaaccatac agcaacccat ctactgcaca aggcattgaa agaagtactt
    27601 ggcgagcatg caaatcaagc tggttcttta gtcgctcctg gacatttacg attcgacttt
    27661 acccactttg gtcaaatcac ttcagatgaa ttagctgaaa tggaaagaat cgtcaacgaa
    27721 aaaatctggg aagctatccc agttgaaaca attgaaacag atatcgatac agccaaaaac
    27781 atgggtgcaa tggctttgtt tggtgaaaaa tacggacatg acgttcgagt agtaaatatt
    27841 ggcggctggt caatcgaact gtgcggaggc acacatgtag caaatacaga aaacatcgga
    27901 atcttcaaaa tcgtttctga atcaggaatt ggtgcaggcg tgcgacgaat cgaggctgta
    27961 acaagtaaag aagcgtacga actacttgaa gaagaagaaa aacaactaaa acaagtagca
    28021 acaattgtta aatcacctca attgaaagaa gttgtttcaa aagcggaaca attgcaacag
    28081 caagtaagag agctacaaaa ggaaaatgaa caattagcta gcaagttagc gaatcaacaa
    28141 gctggtgatg tattcaaaga tccagaaacg atcaatggaa aaacaatcat tgcagcacaa
    28201 gtaaaagtga aagatatgaa tcagctgcgt caattagctg accaatggaa acaaaaaaat
    28261 atttcagata ttcttgtttt agcaaacgaa caagaaggaa aagtcagtct gctagcggca
    28321 atgacgaaag aatcaaatga agctggacta aaagctggcg acttgattaa agcaatcgca
    28381 ccgaaagtag gcggtggcgg tggtggacgt ccagacatgg cacaagctgg cgggaaaaat
    28441 cctgctggta ttccagatgc acttaaagct gcgaaagaat gggttgcgca acaatagggc
    28501 gtttacgttt gacagaaaaa caaaagttca aacattgaac gaaacatgat tatgggggcg
    28561 aagacaaaca agtcttgccc tcatactttt ttcaattgga tggcgcatct aatcaaatgg
    28621 tcggattcgg atggtcttta gttgacgaaa gtgacagaaa tttttacgat tagtataaat
    28681 aaaaaggggt gaaaaaatga atagtagaaa taagaccttt cgactagtct tacgtgcgat
    28741 cttgttagca atcatcatcg tacaagcgat ggttccttgg ctggggttta ttccattagg
    28801 attcattagt ttaacaatta tccatatcac agtaattatt gctgcagtag tattaggccc
    28861 aaaagatggc atggtgattg gcttgttttg gggaatcgct acaattgttc gtgcctatgc
    28921 catgccaaca acgccatttg atacattagt gttcacgaat ccgattatct cggtagttcc
    28981 cagagtactt gttggcttag tagcaggctt ggttttccac tggatttatc aaaaaaataa
    29041 atcaataacg atcagctcag cctttgcagg ggtactgggt tcattggtca atacagtctt
    29101 agtgttagga ttcatgggat tattttatac aggtgcaacg gcagatgcct atggtgtaga
    29161 ctctgctctt ttatttaaaa ccttagcagg ggttgcagca atcaatggga taccagaagc
    29221 aataggtgcg ggaatcataa caccattact agcaaaagcg ttatttgcag caacaccgtt
    29281 gaaaccagaa taatgaccat cttttaatag taataggacg cacgatagat agaaaaagga
    29341 agagaggaga ggaaaacaat cgtcctctcc atctttttac ctgagcaaca agcaaactag
    29401 aaggggtaaa aaacttggct aagaaacaag aagaaaaaaa gaaacaaata ccgcaacagc
    29461 ggacaaaaaa aggagtacta gcgggtatct taggattggt tacgaatctg atcttgtttg
    29521 tctctaagct aatgatcggg cttttttcag gaagtgtctc cattatggct gatgcagtga
    29581 acagtctatc agatactgct tcatccatat tgactttgat cggatttaaa attgctgcaa
    29641 agccagcaga tcaggaacat ccttatggac acgaacgctt cgaatatatc agtgggttat
    29701 ttgtttcgat catcattact tatgtaggat ttcaattttt agagtcttct gctgaaaaaa
    29761 tcatccatcc agaaaatgtc tctttaacac ccatcgtctt ttttgtccta atcatttcca
    29821 tctttttgaa atttgcacaa ggcaaaatgt atcaggttat tgcgcaatcc attgattctg
    29881 aaacattgag agctacagca acagatagtt ataatgatgt ctttactaca ctggctgtat
    29941 tgctttcagc agcaatcgaa tgggtaactg gatggcgtat tgatggatat ataggttttg
    30001 ctttagcatt atatatcctt tatagtggat tgacgatgct tcgaaatttt gtatacgaac
    30061 tattaggaag tcgcccaacg ccagcagaga ttgaagaaat ggaagagcat ttaaatgctt
    30121 gcgatgatat acttggctat catgatttat tggtgcataa ctatggaccc aataaacgct
    30181 ttgcatcagt ccacatcgaa gtagatgaat caatgaattt gaatgtggct cataagatta
    30241 ttgatctgat agaaaaagac tttaaaaagt ctttggatgt ggaacttgtc tgtcacttgg
    30301 atccaatgcc tgttgataac gaaaaatact ataatatctt gcggcagtta aagcaaattc
    30361 ttcttgaaac aggtacaggg cttaatattc atgattttag agtaattcat caaggaaaaa
    30421 cgctgcaatt tgatgtagtg gtccctgaga aattccagtt tgttgataca gaactagaaa
    30481 gaatcttacg tagtcaaata gaagaaaaaa tcggagatta ccaattggaa attacgtttg
    30541 atcacaatta tcttttatat taaatgagaa actataaaaa gactccttaa agtttttaat
    30601 ttaaagagtc tttttttctt ttaattgttt atggagagcc tgacgtgcaa gattatctgc
    30661 tcctttattt ttgttttcgg gcaaccattg caggatcaat aaagaaaatt cggatagaag
    30721 ttcttggatt ctttgaaggt agggaaggaa gagctcgttc ttcgttgcgt ttttgtcgac
    30781 cgtagaaact agtgttttgc tgtctgaaaa acagaaaatc gtttcattct gtaagtcgtt
    30841 tttctttaga aattctaaag tttttaaaaa gacttcgaac tctgcttgat ggtttgtccc
    30901 tatagaaagc gggatagata gctgttcttg tttaccttga tagacaatca agattccacc
    30961 tccactagga ccagggttgc cttttgtaga ggcatcaatg tatactttta acataaaaag
    31021 agattctcct ttttgttcaa actaatgtta tggtaagatt atatcattac tgaatcggtg
    31081 agaggttaga gaatgaaaaa aaatattcga tggcagcctg aactggcaca tacgatcatc
    31141 tattggtcat tgaccttgat cattctgttt tttagtctga ttctttcttt agaaaacaca
    31201 ggtccttatt ggaaaagcaa ttttgtttta ggtatttttc ttgtttttct atttctaggt
    31261 ttgcggcgtt cttttatttt aaaatcaaac tctgtgatta tacgttatgc tgctttttgg
    31321 aaagaccgag aaatatctgt cggacagatt cgatctttga cgccaactaa tactggggtg
    31381 caaatgatgt tgcaagatga gaaagcccca tctgttttca tgatgcgaaa aaaagatcaa
    31441 gtagtccttt ttgatcattt tgaaaaaaac ggacccaaat cagaatcaat tccttatcct
    31501 tatacgaaaa aaagaaaaag ctaattttaa aaatcagaac ctaaaaaaac gaatttttac
    31561 aggaaaagaa aagagtgaga cgtacggttc atcccgttta gcgtctcact ctatttgttt
    31621 atctattcat gcatactagt atcggtagtc tgaacgttgc ctttcttgac atgggcagct
    31681 tgtgttcctc gattcccttc gatgatctca aattcgacca cttgattttc ttccaaagtt
    31741 ttgaatccct cttcttcaat cgcagtaaaa tgtacaaaaa tttcttctgt gtcatcataa
    31801 ctgataaagc cgtatccttt tttgttgtca aaccatttta caattcctgt ggtcaaagca
    31861 ctcacgacct ttctaaagtc tgatcatagc cttcattata aagagtattt ataagtacgt
    31921 caaataaatt aaaaacctgt aattttatgt tttttttctg aataaaaaaa ggtataattg
    31981 gcatatctta acaaagaaga tattttgttg aataaagttc gtgaaaaggg gtaatcaaat
    32041 gaagaatgat ctgcaaattt cacaagaaac agagctagaa ccgattgttg aagtggctag
    32101 taaattagat cttaaggaag acgatttgga actgtatggg aaatataagg cgaaaatcaa
    32161 tttttctgga atcaatcgat caaaggataa cgctgagggg catttgatct tagttacttc
    32221 catcaatcca acaccagctg gcgaaggaaa gtcaacgatc actgtagggt taggagacgc
    32281 gctcaataaa atcaataaaa aatctgttat tgcattgcga gaaccctcac ttggtcctgt
    32341 gatgggaatc aaaggtggtg ctgcaggcgg aggatatgca caggtattgc ctatggaaga
    32401 tatcaacttg cattttactg gtgacatgca tgctatcaca acagctaata atgcattatc
    32461 tgctctttta gataatcata ttcatcaagg aaatgaattg aacatcgatg cccgccgagt
    32521 gatttggaaa cgggtagtcg atctcaatga tcgtgaactg cgtaaagtaa ttgttggctt
    32581 gggtgggcca atccaaggtg ttccaagaga agatgggttt gatatcacag ttgccagcga
    32641 aatcatggct atcttgtgtc ttgcggcaga ccttgaagat ttaaaggctc gtcttgcaag
    32701 gatcgttgtc ggctatacat ttgaccgtca gcctgtgact gctggagact tgaaagcaga
    32761 aggcgctctg gcattactgc tgaaagatgc aatcaagccg aatcttgtac aaacaattta
    32821 cggaacacca gcttttgtcc acgggggacc gtttgccaat atcgctcatg gctgcaatag
    32881 tattttagct accaagacag cattgagatt agcagattac gttgtcacag aggcaggatt
    32941 tggtgcagat cttggcggag agaaattttt agatatcaaa gtacctaact tgaaaaaagc
    33001 gccggatgct gtggtgattg tcgccactgt ccgtgcattg aaaatgcatg gcggaatgaa
    33061 aaaagatgaa cttaaaaatg agaatcttga tgctttgaaa gtaggttttg caaatttaaa
    33121 gcgccatata cgtaatatgg aacaatatca gctacctgtg atcgtcgcaa tcaatgaatt
    33181 cgtaacagat acagacaatg aactcacact gttggagcac ttgtgtgaaa atcaggggat
    33241 tcttgccaag agagcgagtg tatgggcaga tggagcagaa ggcggcgttg atttagcaaa
    33301 agctgtagta cgattgattg atagaaaaga agctaattac aagccattat accgcctaga
    33361 agaaacgata caagaaaaga cagaaacaat cgtcaagaaa atttacggag gaaatggtgt
    33421 tgttttcagc aaaaaagcgc aaaaacaaat agaagagttt acgaagaatg gatgggacaa
    33481 actgccgatt tgtatggcaa aaacacagta ttcattttct gatgatcctt cactgctagg
    33541 tgcaccggaa gatttcacca tcacgatccg cgaaatcatc ccaaaattag gggcaggatt
    33601 tttagtagcc ttgactggag atgttatgac gatgcctggt ttgcctaaaa aacctgctgc
    33661 attgaatatg gacgtgacaa atgacggaga agtgttggga ttgttttaag cttgattata
    33721 atctaccatc gaaggagctg tattttgact acagcttctt tttttagacg atagggaatt
    33781 aattttgttt acactaggat cattcttttt ctttattgta ttttgagcta tactaaagca
    33841 taataggagg gtatgatgaa aacgacattt ataagaaaaa ccggttttta cggtatgggt
    33901 agctcgattc agctaagaat caacaatgaa aaacgcagtg ttctcagtca tgaacaaaag
    33961 ataacgcttg atctggaagt gccctttacg atgcaggtca ccttttattg gctaaaaagc
    34021 cccgtttata cagtgaaaga agcaaagcct gcttatgtga tcacaatgaa ttacttgatt
    34081 cttcaaatct atccgttttt atttctagca actgggttgg gggccgtttt cctacaaaat
    34141 ttatttttca gtttttttgc agttattgtg ttgattgcgt tctttttcta tataaaaaac
    34201 cgggcatatt tgatcaagga ggaaaattat ggcaaactct gatctttttt taatcagttt
    34261 caatcgaatc gaaaaatgga tgagagacga gatgggaaat gcacgaaata tgggatttac
    34321 cgaacttgtc agacgcctag ctcaaagaaa acaattaatg atcagaaaat acgaagatga
    34381 tcttttacag cttgctcagt taaggaatgc gatcgtccat gatcggatcg cagtcgattt
    34441 tgtcattgca gagccaaatg attggacagt caagcgaatc cagctgatcg aacaagaact
    34501 gattcagcct gaaaccgtgc tgcctcgatt tgcaaaacgt gtaacaggtt ttgaacaaga
    34561 tcttccgtta cttgaactgt taaagatagt agcagaaaaa aggtattcac aatttccgtt
    34621 atacaataaa ggaaaatttg tagcattgat tactttacgt aatatcgggt tttggctagc
    34681 aaaagaaagc caaaaaggtc cggttgattt aacaaataaa cgagcgttgg atttgatcat
    34741 ccaaaacgga aagtacacaa attatcattt tgttccagct aaaacacata tttacgaggt
    34801 tgaagcgatg tttcgtgagc aagggacttt ggaagccgta cttatcacta aagatgggaa
    34861 tcccgatggt aatctgctcg ggatcgtacg acctcgtgat atttataaac aaatagaaaa
    34921 ggattgattt tttgttaatt atttattgga tattcagcgc tttgctagta ggagtggatc
    34981 agttggtcaa atggtggatc actgataatc tagcgcttgg agagacgaaa aatcttatac
    35041 caggtatttt gtcactgaat catattcgaa acactggagc cgcatggagt atgctagaag
    35101 ggaagatgtg gttctttact atagtgacac tcattgcagt cgttgtgatt ctcacattga
    35161 tgattaaaaa tcgtgaaaaa ggtaatcgct ggttcatgat tggattgagt ttgattctag
    35221 ctggtgcgat tggcaatttt atcgaccgta ttcgtcttgg ctatgtagta gatatgttcc
    35281 agacagattt tatgagcttt ccgattttta acgtagcaga tgtgacactt gtatgtggag
    35341 tgatttgtat gttaatttat attattttag atgaaaagga tcaaagaaaa aaatgacaga
    35401 acttaaaacg acgattcacc atgaatctgg taggatcgat aaagtattga ctaatttatt
    35461 caccgattac agtcgttcgc aaattcagct gtggctgaaa aatggagcgg tttctgtcaa
    35521 tggacagcca gtcaaagcaa attacaaagt aaaacaagga gacgagatta ctgtcttgat
    35581 ccccgaacca gaaacattag atgtggtccc agaaaatatc ccgttgacga tcgtttacga
    35641 agatgaagat gtagcggtga taaacaaacc tcaaggaatg gtcgttcatc cttcagcagg
    35701 tcatacgcat ggcacaatgg tcaatgcgct gatgtaccat atcaaggatc tttctacgat
    35761 caacggcgtt attcgtccag gtattgttca ccgaatcgac aaagatactt ccggactgct
    35821 aatggttgcc aaaaatgacc aggcacacga atcattagct cagcaattaa aagataagac
    35881 ctctttgcgc aaatatgttg ctttagtcca tggagagatt cctcacgaaa aaggaaccat
    35941 caatgcgcca atcggccgtt cgaaggaaaa ccggaaaatg caggcgataa gagaagatgg
    36001 gaagtcggct gtcacacatt ttactgtatt ggaacgtttc gaaggattta ctctggttga
    36061 attaaaacta gaaaccggaa gaacacacca gatccgtgtg catatgaagt atatcggtta
    36121 tccgttagct ggagatccag tatacggacc gaagaaaact ttgaaaggca atggtcagtt
    36181 tctccatgcg caaatgttag gttttacgca cccgactaca aacgaaaaga tgatctttga
    36241 agcacctctt ccagaagttt ttgaaaaaac tttagaaaaa ttaagaaaaa acattgcatt
    36301 ttaatttaga taacggtata gtaattaaca acgaaagcat aaaacttaat agaattcctt
    36361 taagtttagt cccgtgaggc taagaagggt gggagcaagg atacgtatgg tgcgccataa
    36421 gtagattgct cattgacata gtatagctat cagtgtatca gtatacgctc agtctaaaca
    36481 ttcctcctgt cctcttaggg caggagtttt tttatgcaaa aaagtagggg gaatgaaaat
    36541 gcaagcaaaa gaagtagtag atcaggtaac catgaagcga gcattgactc gaattactta
    36601 tgagattatc gagagaaacc atagtatcca agatatcgta ttggtaggta tcaaaacacg
    36661 aggaatctac attgcatcaa gaattgccga acgcctgaag cagctagaag atatcgatat
    36721 tcctgtagga gaactggata tcactttata ccgagatgac aaaaaagaaa atccagaaga
    36781 accggaactt cattcttctg acatcccagt ttccttagaa ggaaaagaag ttattttgat
    36841 tgatgatgtt ttgtatacag gacgaacgat tcgagcagca atggacgcag taatggattt
    36901 tggcagacca agaaaaattt cattagcagt cctggtcgat cgtgggcatc gagaattgcc
    36961 aatccgtgca gattatgttg ggaagaacat tccaacatct cgggcggaag agattttagt
    37021 agagatgcaa gagcttgatg gacaagatcg catcatgatt ttgaaagagg aagattagag
    37081 gagagagaaa catgtcggag aaaaaaggat ttcataatga tgacgtggtg ttagatatcc
    37141 acgatcgccc aaccccagga cattgggtag gattaagttt acaacacttg ttcacgatgt
    37201 tcggagcaac cgtgttagtc cccatcttag taggtatcga tccaggtatt gcgctagtca
    37261 gttcaggact aggtacattg gtgtacttat ttactactaa aggaaagata cctgcgtact
    37321 taggaagcag cttcgctttc attgcagcga tgcagctttt gatgaaaaca gatggttatc
    37381 cagcgattgc acaaggagca gtcacaactg gattagtcta tttgattgtt gcgctgatca
    37441 tcaaaaagat cggttcagca tggctggata aaatcttgcc tccaatcgtc gtcggtccag
    37501 tcatcatggt tatcgggttg ggacttgcgg caaatgcagc aacaaatgct atgtataatc
    37561 agggcacgta tgatttcaag tatattgctg tagcgctgat cacattagga ttaacaattt
    37621 tcttcaacat gtggctaaga ggttttctcg gattgattcc gattttacta ggtattatta
    37681 gcggatacat cgtagccttg ctgtttggaa tcgtggatac taagccaatc gtagacgctg
    37741 cctggtttgc tgttcctaac tttgagattc cctttgtaca atatgaacca aagctatatc
    37801 ttggagcaat cacaacaatg gcacccatcg cttttgtgac gatgacagag catatcggcc
    37861 atcttatggt attgaataaa ctaactaaac gtaatttctt tgaagaaccg ggtcttcata
    37921 agacgttgac aggtgatgga ttagcacaaa tcgtggcagc ctttgtcgga ggtcctcctg
    37981 taacaagtta cggtgaaaac attggtgttc tggctatcac acgtgtccat agcgtgtttg
    38041 tgatcggtgg agctgcagta tttgcagtca ttttaggatt cgtaggaaaa ttaagcgctt
    38101 tgattttaag tatccctgga ccagtcatct caggaatcag ttttatctta tttggagtaa
    38161 ttgccgcaag tggaatgaag attttaatcg agaacaaaat cgattttgat aaaaagaaaa
    38221 atctgttgat tgcttcagtt atcttagtag taggaattgg cggactcgtc tttgaagtag
    38281 gcacatttac attgtcggca atggcattag caactgtact tggcatcatt ttaaatctta
    38341 tcttaccaga aacttcacga agcgaagaaa actaaggagg aaacattatg atcatcaaat
    38401 cagaacgtat cagcttaaag catttattga ccgttgaggc acttactgat caagaagtta
    38461 tgggcttgat ccgcagaggg caggaattca agcaaggtgc taattggact ccgcaaaagc
    38521 gccagtactt tgctacaaac ctctttttcg aaaatagtac tcgcacacat aaaagcttcg
    38581 atgtagcgga gaaaaaacta gggctagaag tgattgaatt tgaagcaagc acaagctctg
    38641 tccaaaaagg ggaaacacta tacgatacag tactgacgat gtcagcatta ggcgtagacg
    38701 tagcggttat ccgtcatggt gatgagaact attatgacga actgatacaa agtaaaacaa
    38761 tccagtgttc gattatcaat ggaggagacg gaagcggaca gcatccaacc caatgcttgc
    38821 ttgatttgat gaccatttat gaagaatttg gtcattttga aggtctgaaa gttgccatcg
    38881 taggtgatat cactcactct cgcgtagcta aatccaatat gcagatgtta aaacgtctag
    38941 gcgcacaagt attcttctca ggtcctagag aatggtacga cgatgaattc gaagtgtacg
    39001 gacattatat gccattggat gaactactag atcaagttga tgtcatgatg atgcttcggg
    39061 tgcaacacga acgccatgat ggaaaagaaa gtttctcaaa agaaggctac catcaggaac
    39121 atgggttaac agttgagcgt gcgaagaagt tgcaaaaaca tgcaatcatt atgcacccag
    39181 caccagttaa ccgtgatgta gaattagctg attcattagt ggaaggatta cagtcgcgga
    39241 tcgttcagca aatgagcaat ggagtattcg tcagaatggc aatcttagaa gcagtgctaa
    39301 atggaaaagc gtaattatag gaggatgaaa aaatgaaaac actcattaaa aacggtcaga
    39361 tcaatacacg aaaaaatatg acgacaccag cagaaatttg gatagaagat gggcgaatca
    39421 aagcaatcgg tacaggattc tcagaagccg aatttgatga agtgtttgat gcaaagggac
    39481 aattgatcac acctggttta gtggacgtcc atgttcattt gagagagcca ggatttacat
    39541 acaaggagac gatcgaagct gggactcgct cagctgctcg tggcggtttc actactgtat
    39601 gcgcgatgcc gaatttaaat cctgtacctg atacggcaga aaagctgaga caagtatatg
    39661 acatcattag aaaagatgca gttgtgaagg tattgcagta tgcgccaatc acagaaaatc
    39721 tgcgaagtga gaaattagtt gatcaagaag cactgattga agaaggtgct tttgctttca
    39781 caaatgacgg tgtaggtgtc caaacagctg ggacgatgta tctggctatg aaagaagctg
    39841 caaaaaacaa taaagcccta gtagcccata cagaagatga atctttatta tttggcggcg
    39901 ttatgcacgc tggcaagaag gcagaagaac ttggtcttcc cgggatctta agcgtaaccg
    39961 agtcttctca aattgctaga gatctgttgc tagctgaagc aactggtgtt cattaccatg
    40021 tttgccatgt ttctactaaa gaaagtgtcc gtgtaatcag agacgcaaaa aaagcaggaa
    40081 tccacgtaac agcagaagtt tcaccccatc atcttatttt gatcgacgaa gatattcccg
    40141 aagatttcgg cttttggaaa atgaacccgc cgctaagagg acgggaggac agagaagctt
    40201 tgatcgaagg cttgcttgac ggtacgatcg attgcatcgc tacagaccat gccccccatg
    40261 gattagaaga aaaaagccaa agtttcatga aatcaccatt tggtatcgta ggaagcgaaa
    40321 cagcgtttca actgatctat actcattttg tagaaactgg tcgattcacg ttggaacaag
    40381 tgatcaattg gttggcagtt aaaccggctg agatttttgg tttgaatgca ggaacgctaa
    40441 cggttggtgc ccccgctgat gtagcagttt ttgatattac acaaacttgc acgatcgata
    40501 aagaagactt tttatcaaaa ggggagaata caccttttat cggctggaaa gtaaaaggcg
    40561 aaacacaaat gacttttgtc aacggaaaac ttgtgtggca gaagggagaa taaaagtatg
    40621 gaacgtttgc ttatattaga agatggaaca gttttcgaag gaaaggcttt tggtgcagat
    40681 gctagtgtct tcggagaaat cgtttttaca acaagtatga ctggttatca agaaactatc
    40741 actgatcaaa gttttaacgg tcaaatcatt acatttacct atcctatggt gggtaattat
    40801 ggggtaaata gagatgatta tgaatcgatt tctccaacat gcaaaggagt agtagtaaaa
    40861 gaacatgcac gtgtagcttc aaactggcga aaccaaatga cgctagattc atttttgaaa
    40921 caaaaaggaa ttcctggaat atcaggtatc gatacgcgcg cccttactcg caaaatccgt
    40981 gaagtcggaa cgatgaaagc tagtatagtt gatgctggag attccttcga acatgcattt
    41041 gatcagctga aagcaaccgt tctagcaacc aatcaagtgc agcaagtctc aactaacaaa
    41101 ccttatccaa gtccaggaac aggtcggaaa gtcattgtca ttgacttcgg cttgaaacat
    41161 agtattttac gagaattgag taaaagaaac tgtaacctca cagtacttcc ctataacaca
    41221 gatgccgaga cgattttagc gctatcacca gacggagtca tgctgacgaa tggacctggt
    41281 gatccaaaag atgtgccaga agcaatcgaa atgatcaaaa aaatccaagg aaaagtaccg
    41341 atttttggta tttgtctcgg tcaccagtta tttgctttag ccaacggtgc agatacctat
    41401 aaaatgaaat tcggacatcg aggattaaat cacccggtac gagaagtagc gaccggaaga
    41461 attgatttta cttctcaaaa tcatggctat gcagtcgatg aagccacgat tgatccagaa
    41521 aaactcatcg taactcatgt agaagtaaat gacggaacag tagaaggagt caaacacaga
    41581 gattatcccg ctttcagtgt gcagtttcat ccagatgctg cccctggtcc gcatgatgca
    41641 ctacatttat ttgatgaatt tatggaaatg atggacgcag gaaaggagca gtaaacagat
    41701 gccgaaacgt acagatatca agaaaattat ggtgattgga tctggtccga tcatcattgg
    41761 tcaagccgca gaatttgact acgcaggaac acaagcctgc cttgccttaa aagaagaagg
    41821 atatgaagtg gttttagtaa attctaatcc tgcgacgatc atgacggata aagaagtggc
    41881 agatcaagta tatatcgaac cgatcacact tgaatttgta tcaaggattc ttcgaaaaga
    41941 gcgtccagat gctctccttc caactttagg cggacagaca ggcctcaata tggcaatgga
    42001 gctggcagca tcaggtattt tagaagaatt gaacatagaa cttctaggaa caaagctttc
    42061 tgcgatcgat caagcagaag atcgtgatct attcaaacag ttgatgaaag aattgaatca
    42121 gccaatcccc gagtctgaaa tcgtcaatac agtagaagaa gctgtagatt ttgcaaataa
    42181 aatcggctat cctgtcatcg ttcgtccagc ctttacttta ggcggaactg gtggaggtat
    42241 gtgtgaaaat gaagaagaat tacgtatcgt atcagaaaat ggattaagtc tttcccccgc
    42301 tacccaatgt ttgattgaac gaagtattgc aggttttaaa gaaattgaat atgaagtgat
    42361 gagagattcg gctgataatg caatcgttgt ctgcaatatg gaaaattttg acccggtggg
    42421 tatccataca ggtgattcta tcgtatttgc ccctagtcaa acattgtctg accatgaata
    42481 tcaaatgctt cgtgatgctt ctctttcgat tattcgagca cttaagatcg agggaggctg
    42541 taatgtccaa ctcgctcttg atccacatag ttttaattat tatgtgattg aagtcaatcc
    42601 gcgtgtttct cgttcatctg cgttagcaag taaggcgact ggctatccga tcgctaagct
    42661 agctgctaaa atagcgcttg gattaacatt agacgaaatg aaaaatcctg ttacaggaac
    42721 gacttttgca gaatttgaac ctgcattaga ttatgttgta gctaaaattc ctcgctggcc
    42781 gtttgataag tttgaaaacg gagaacggct tttaggcacg cagatgaaag caactggtga
    42841 agtgatggct atcggtcgga atatcgaaga gtctttattg aaagctgtgc gttctttaga
    42901 aatcggtaca caccatatcg aacttcctga gttggcagac atcacggacg atgacctaat
    42961 ccaaaaagtg gttcgagcac aagatgatcg cctgttttat gtagctgaag ctattagaag
    43021 agggtacacc atagaagaat tagcggattt gacaaaaatc gatttattct ttttagataa
    43081 actgttgcat atcgtagaaa tcgaacagca gttgaaacag cagccgttgg atgacaagat
    43141 attgaaaaca gcgaaacaaa acggttttac agacaaaaaa gtggcggagc tgtgggaaat
    43201 aaacgaagaa gccgttcgcc agatgagaaa agaaaatgat ttgattcctg tatataagat
    43261 ggttgacact tgtgcagcgg aattcgaatc aaaaacacct tatttttata gttcctatga
    43321 atatgaaaat gaaagttcac gatcagaaaa gccctcaatt ctagtactgg gatctggtcc
    43381 tatccgtatc ggacaagggg ttgaatttga ctatgcgact gtgcattctg tcaaagcaat
    43441 ccagcaggca ggatacgaag caatcatcat gaacagcaat cctgaaacag tttcgactga
    43501 cttttctatt tcggataaat tatattttga gccattgacc ttcgaagatg tgatgaatgt
    43561 gatcgagcta gaacagccga taggagtaat cgttcaattt ggtggtcaga cagcaatcaa
    43621 cttagccgaa cctttgactg ctgctggtgt aaaaatctta gggacaacga tcgaagactt
    43681 ggatcgtgcg gaaaatcgtg atttattcga gcaagcgctg aaagagttgg atattcctca
    43741 acctttaggg gatactgcta caagtaaaga agaagccgtg gttatcgctt cgagaattgg
    43801 ttatccagtt cttgttcgtc caagctatgt attaggcgga cgtgcgatgg aaatcgttga
    43861 aaaccagcga gatttagaag attacatgga acatgctgtg aaagcatctc cagaacatcc
    43921 agtactagtg gaccgttact tgcttggtgg tgaatgcgaa gtggatgcga tttgtgacgg
    43981 tgaaacagta ctcatcccag ggatcatgga gcatattgag cgtgctggtg tccattcagg
    44041 ggattcaatg gctgtgtatc cacctcaaac attatctgaa tcgatcaaga aaacgatcga
    44101 agattacacg atccgcttag cattaggttt gaattgtgtc gggatgatga atatccagtt
    44161 tgtcatccat gaaaatgagg tttatgtgat cgaagtcaat cctcgagcaa gtcggactgt
    44221 tccattctta agtaaggtga caggaattcc aatggcacaa attgcgacga aagctatttt
    44281 gggagagaaa ctaaagacac ttggttatga aaacggactt tatccagaaa caaaagaagt
    44341 tcatgttaaa gcacctgtct tttcttttac gaaattgcag aaagttgata cttacttagg
    44401 gcctgaaatg aaatcgactg gagaagtaat gggatctgac agacaattag acaaagcttt
    44461 gtacaaggca ttcgaagctt ctggattaca cttgcctgaa tatggagccg tattatttac
    44521 gattgcggac gaaacaaaag aagaagcact agaattagct aaacgtttta cagaaattgg
    44581 ctatagtctg ataggaacga aacatacagc tgcttatttc gaaaaaaatg gtctggtcgt
    44641 tgaaacagta gcgaaaattt ctgaggaagc agcagaaaaa aatgtagtcg acttaataag
    44701 agatggtaaa acacaagtgg ttgtcaatac gattgataaa gatcggggaa atgcttctaa
    44761 agacggtttc atcatccgaa gagaagcagt tgaacatggt attccattgt ttacttcttt
    44821 agacacagca gatgcgatcg ttcgagtgat ggaatcccgt gctttttcaa ctaaccctat
    44881 ttaacggcga gcacatgtta agacaaaaag acgaaaaacc gttgcaggag gggacagatg
    44941 aaacaagaaa tgatggaaat cgttcagcaa aaacagctgg caccaaagat ttttgaaatg
    45001 acattgaaag gtgatctagt aaaagaaatg cagcaaccag gacagttctt gcatattctc
    45061 gtgccgcgtg cagacttgct attacgccga ccgattagct tgaatcatat cgaccacgaa
    45121 acaaatactt gccgtatcat ttatcgggtg gaaggtgagg ggactagagt cttctctact
    45181 ctttctgccg gagaccattt agatgtcatg ggtcctttag gcaatggttt tgatttgtcc
    45241 aatcttcata caggagacga agtatttata atcggtggag gaatcggtgt ccctcctcta
    45301 tatgagttat caaaacagtt agtcgaaaaa ggaattaagc caacgcattt tttaggtttt
    45361 gccacacatg aagtcgttta ttatgaaaaa gaatttaatc gtctaggaga aacaagaatc
    45421 gctactgacg atggaacata cggtatgcac ggaaatgtcg gtaatttatt gttaggaacg
    45481 aagacggaac ctacagctgt ttttgcatgt ggttcaaacg ggttattgaa gacagtggaa
    45541 cagctgtttt cttcgcatca aaatgtccag ttatcattag aatcccgcat ggcatgtggg
    45601 ataggggctt gctatgcttg cgtttgtcat acgccggaag atgaaacagg aacaaaaagt
    45661 gtgaaagttt gtgacgaagg accgattttt aaaataggag aggtaattat atgaatgaga
    45721 atcctttagc ggtcaaactt cctggcctag aattaaaaaa tcctatcatg ccggcaagcg
    45781 gctgtttcgg ttttggcaaa gaatacggaa aatactatga tttgaatcag cttggatcga
    45841 tcatggtcaa ggcaacgact ccaaatgccc gctttggcaa tcccacgcca agagtggcgg
    45901 aaacaccatc tggcatgttg aatgcaattg gtttgcaaaa tcctggtcta gacgttgtta
    45961 tgcataaact tcttccagaa ttagaggagt acaatgagtt acccatcatc gcaaatgttg
    46021 ctggtgcttg tgaagaagac tatgtagaag tttgtagcaa aattggcgaa gcaccaaatg
    46081 tgaaagcaat cgagctgaac atttcttgcc caaatgtcaa acatggcgga attgcttttg
    46141 gaacagattc tgatgttgct tttcaattaa cacaagcagt aaagaaagtt tcttctgttc
    46201 ctgtctatgt caaattgtcc ccaaatgtaa cagacatcgt tccaatcgct caggcaatcg
    46261 aagctggagg ggcagatggg tttacgatga tcaatactct tcttggtatg cggattgacc
    46321 ttaagacaag aaaaccgatt ttagccaatc aaacaggtgg actctccgga ccagcaatca
    46381 agcctgtcgc tattcgtttg attcaccaag tagcaagtat atccagcctt ccaatcattg
    46441 ggatgggcgg cgtgcagaca gtggatgatg ttcttgagat gtttatggct ggagcaagtg
    46501 ctgtagctat agggaccgcg aatttcaccg atccgtatat ctgtcctaaa ctaattaaag
    46561 agctccctgt gcgaatgagc gaactaggca tcgaatcgct ggaaagatta atcaaagaag
    46621 tgagagagga acgtgaacga tgaatcaact agaccaacga ccaattattg cattagattt
    46681 ttcaactagg caagaggtag aagactttct tcgatttttc ccgaaagaag aaaaattgtt
    46741 tgtgaaaatc ggaatggaat tgttctacca agaaggacct gaaatcgtac gttatctaaa
    46801 agaggctggt cataatgtat ttttagatct gaaacttcac gatattccga atacggtcga
    46861 aaaagcaatg cggggattgg caaagcttgg tgttgatctc acctgtgttc atgccgctgg
    46921 cggtattcgt atgatggagg cggcaatgcg aggattagaa gaggggactc cagaaggtgg
    46981 aaaacgtccg ctacttctag ctatcactca gcttacttct acaagtgaag aagaaatgca
    47041 tgccgatcaa ctaatcgaag tttctttaga aaaaagtgtg atccattatg ctagctgtgc
    47101 caaaaaagca ggattagatg gtgtggtttc ttctgcttgg gaagtagaag cgatcaaaga
    47161 aatggcagga gacgaatttg tttgcttaac tccgggtata agaccagaag gaacagtcgc
    47221 aggagatcag acaagagtag tcacaccttc tcaggcaaaa aagatcggct caacttttat
    47281 cgttataggt cgaccaatca ctcagtcaac taatccttat aaagcatatc aaacgattca
    47341 aacagaatgg agctaaccta aaatgaatgt tgaacaaagt atcgcaaaag acttattaga
    47401 aattgaagca gtatttttaa atccaagtaa tccttttaca tgggcaagtg gaatcaaaag
    47461 cccaatttac tgtgataacc gaatcacgat gagctatcca aaagtccgaa aagagatcgc
    47521 aaaaggatta gcaagcaaaa tcaaagaagc ttaccctgaa gtacaagtga ttgctggaac
    47581 tgcaacagca ggaatccctc atgccgcatg ggtagcggaa atactggatt tgccaatggt
    47641 ctatattcga agtaaggcga aagatcatgg gaaaggtaac caaatcgaag gacgtatatt
    47701 tgaaggtcag aagatggttg tgatcgagga tttgatttct acaggcggaa gtgtcttgga
    47761 agcagctgaa gcagcaaaaa gagaaggtgc ggatatcctt ggtgttgcgg ctatctttac
    47821 gtatgagttg ccaaaaggaa aagcaaattt cgaaaaagcc gaaatccctc ttatgacgtt
    47881 gaccaattat tctgtattga ttgaagcagc tttagaagat cgttatatcg atgagcaaga
    47941 attaactctg ctaaaagaat ggaaaaaaga tcctgaaaat tggcagattt aagcgcgctt
    48001 gatgaagata gagaaatccg atttgtgtct ccagaatcat caattggaat agcaaaaatc
    48061 caaaaactga ttgcgagaag catcttttca tcaggtatga aaagaaactt ctatcgtgat
    48121 cagtttttga tttttttaaa attcattttt ctaagactgg ttagcagaaa gaagagaata
    48181 atctcttctc ttttttctat tatatataga ttttctctat tttttggtaa gggagctgtt
    48241 tcaaaaatgg taaagagcgg aaatgttgcg aatatcagtt atttaatgat atgatattcg
    48301 gaaaaagaaa tctcaaaggg aggtaacttt tatggggttg aatgaattta ttttaggatt
    48361 gaacaattta gagacgatca caagggcacc tggatttttt aaatatacag agcatacagt
    48421 tgcagcacat tcgtatcgtg tagcatctat tgcgcaagtt ctaggagaca ttgaagaagc
    48481 gaatggacaa ccgatcgatt ggaaactgct ctatgaaaaa tcattgaatc atgactacac
    48541 agagcgtttt attggtgata tcaaaacgcc ggtgaaatac gcaagcaaag agttaagagg
    48601 gatgctgcaa acagttgaag aaaagatgac ggacgaattc atcaaccaag agattccagc
    48661 tgagttccaa gaagtttatc gccgtcggtt gttcgaagga aaagatgaaa ccgtagaagg
    48721 agaactatta gccatttcag ataaggttga ccttctttat gaatcttttg atgaaatcat
    48781 caaaaataac ccggaaaaag tttataagga gatgttcatt gaggcagtga ctacgattaa
    48841 aagctttgaa catcggcctt ctgttcagta tttctttaaa gagatatttc cggaactatt
    48901 gaaccaggaa ttttatggaa aagaggcgtt tttagaaacc gtctcaacga ttgtaaagaa
    48961 agaagaaaaa taaaaatgat atgagatgca gggggaagta tagttgaaaa agaatatgga
    49021 tgtcgaatgg ggctattctg gagacaaagg tccggaaaat tgggcgagtt tatgtgattg
    49081 gtttgcgaga ggtgcggaat ttccattgca gtcgcctatt catttgacca agggggaaga
    49141 aaccaaaata gaaggagaca cgctttcctt tcattatcaa aagcaacggt ttactgacaa
    49201 ggaattcaaa aacacgattc atttagtacc ttttgaccaa ctcagttatg tagagttcaa
    49261 gaaagaacgc tattatttga cagatattca tttccacttg ccttctgaac atatcatcaa
    49321 cggaaaacag gaagatattg aatttcattt tgttcatatg aacagcaaaa aagagaatct
    49381 agttgtgggt gtcatgtacc agctaatggt acaggaaggt tggctgtata atcaagaaaa
    49441 tggggaaagt tggaacttag aaaaacatga gcattgggta aatccggatg tgttctttcc
    49501 ggagcaaaaa agccatttcc attatattgg ctcattgaca actccgccta ccagcgggcc
    49561 aatccaatgg tttgtaatgg atcatgtagg aaaactgaac caagagtttc ttctcccctt
    49621 cgatggtcag tatgctcgac cgaacaaccg accaatccag cccttgaatg gaagagaaat
    49681 ttattatttc gaggagtttg agcaatgaat attcagcaga tgaaatacgt ggtcgctgta
    49741 gcaaacaatg gtagtttcag agaagcggca aagaaaatgt ttgtctccca gccaagtctt
    49801 tcagctggta tcaaagaatt agaaaccgag ttagggatca cgctgtttac tcgaacgaat
    49861 cgaggggcat atttaacaga agaagggcaa gacttcttaa atcgtgccga gaaaatattg
    49921 gtccagcttg atacattaga aactcactat ctaacgagtg aaaagagaga caatttttcg
    49981 atcgctgccc agcattatga tttcttaggt cctttgaccg cacagctact cgaggaattc
    50041 aaagacgaga tcaaacaatt tcggatcatg gagacgacaa cagcgagagt gatcgaagaa
    50101 gtcaaagagc agcacagtga gatcggcatt atttatatga atgagcataa tcaagctggt
    50161 atcagaagat atttacaaca aggagagcta agtggccgca gcattggaaa attcaaaacg
    50221 catatttttt taaggcaagg acatccatta gctgaacacg cagagatcac caaagaagaa
    50281 ttgttatctt atcctcaagt tcgttttaca caggatggaa ataatttccc gtatttttat
    50341 gaagacctta tcgagatccc tgatcaagaa acagtgattt atacgagtga tcgaggtact
    50401 ttgatgaata tcgttcttga aactgatgct tacgcttcag gatctggtat tgtgattgga
    50461 gggattcgcg agcacttgcg gttaatccct ttggcagaag gcgaactaaa tgaattttat
    50521 atcatttatt cggcgaaacg tccgctcagt gaaatcgctc aaagatttat caaagagctt
    50581 actcggttac tggaccagca gagctaagaa aagcagatga gaacgctgtt tattgaacac
    50641 cgttgatcaa aaaagagggg ttgtgacaga attttgtcac aactcctctt tttgtagcaa
    50701 acaatttata actaattgct gttgtgggaa agtacgcaaa tatgattcta gaaacactca
    50761 ttgattacct gttttgtttc atagctgtaa ttttttcttc ttctggtgtc acgataatcg
    50821 tcttttggtt ttcataaata acataaccag gcttggcgcc atttggtttt cggatatgct
    50881 ttacctgtac aagatcaacc ggcacttggg cagagtatcg gtattttgaa aaataggcag
    50941 ctaactcggc agcttctgta attgtttcat ctgagggctt gtcactttta atgatgacgt
    51001 gcgaaccggg tatatttttt gcatgaagcc aatagtcggt ttttttagct gtcttcattg
    51061 tcagctggtc attttgaaga ttgtttttac caacaagaat gagcgttccg tcagaagaaa
    51121 agtattgatc aggctgagat ggtttcttac gtttttgttt ttttaagttc ttctttttca
    51181 ggtatccttc tgaagtcaat tcttctttaa ttgcttcgat atccattggg ccggcaattt
    51241 ccagttgaga taagacagat tcaagatatt ggatttcatc tttggcttct tggatttgtt
    51301 cgccaatcag cttaacagcg ttcttcagct tttggtatcg atggaaatat ttctgtgcat
    51361 tttgatttgg tgtcaatgcc ggatctagtg ctattttgat tggccgatct tcttcataat
    51421 aattaggtaa aacaacttct tttgcgccgc gtggtacttg tgtcagaaaa gtagtaagta
    51481 gttcgccatt tctacggtaa ttctcagcgt tttcggattc tttgagtgtt tgttcacgtt
    51541 ttttcaattt atttttattt cgttttagtt cattttcgat ttttcgaatc agttctccgc
    51601 cttgttgttt tgcccgatct ttctctgctt tttcgtgata atagctatct aataattgac
    51661 tgagtgttgg ataagctgtt acagcagatg cctgttcgga caatacctga taagcaatcg
    51721 gtgtgaaaaa atctttattt tccgtttcgt aaaaagtagg aataggctga tggctgatgg
    51781 ctgagaaaaa ttcgtcccat acaaccatct tttcattagg acgttccagt aatcttttgg
    51841 tcagctcttg agcgatatcg aaaccgattc cctgaaactg acgttggatt gcttttggat
    51901 ctagttcagt ttgggaaagc cgctgaaaga ttttttcttt ctcggagcta aatgggttca
    51961 agacctcttg ttttggcgga gcaacatatt ctacaccagg aagcaaagaa cgataagtgt
    52021 tttgagagct tccaatgtgt ttgatcgcat ccagtatttt tccagtttct cgattaacta
    52081 agacgatcgt actatgacgt cccattaatt caacgatcaa tatgatattt tgtagatcac
    52141 ccaactcgtt tcgtttagca aagtggaaat ggatcactcg atcattttcg atttgttcga
    52201 tgtcttctaa aatagcgcca tccaaatatt ttcgaagcat catcacgaag ttaggcggat
    52261 tatcaggatt ctgataatcg atttgggtaa tctgtacacg agcataactt gggtgcgcag
    52321 ataataataa tcgatggttt ttgccgcgtg aacgaatcac cagcacgatt tcattttcat
    52381 aaggctgatg gattttcgag atccggccag atagtaacgt ttcacgcaat tcgttgacca
    52441 tcgcgtgggt aaatacacca tcaaaggaca tggttcattc ctcatttctt ttcataagta
    52501 tattcattat aacgaaaaaa agaagaaaaa ccaatttggc cgaatagtaa agcaaaagtc
    52561 tagaaaaatc ttggactaat tacataaaaa acagtgtttc tatcgttgaa atccaaattt
    52621 ttaggtataa taaaaaatag gagaaaaagt agaaaagaag gtgtttgtta tttgggaagt
    52681 agttaaatcg agagtacaag aaatgaatga acatttctca gtgtcattcg cagaaaatcc
    52741 aagtaagtat tttcgacgtc ttgtttttct gctcatctta tcattgctgt taacagttgc
    52801 aatgttttta ttgccggaaa aaatccgaga agagcaagta aatagtctcg tttattcgtc
    52861 cataccaacc agcaaattga aaccttttga ttacagcaaa gctgatcaga aaatcaaaca
    52921 aagccgggcg atatcggtga tgttcagcaa accgaacggg aaaacctatc aggacatgct
    52981 tgatgttttt aatgatcctg agcttatgga agaattgaat cgaccgatct tttattatcc
    53041 gatcgtttat gatgtacatg aactggaaca aaaatataac atacgaacgg atgaagtgac
    53101 atttattttc tttgatggag gaaaagaagc aaatcggata actgcgggta agggaggaat
    53161 aactgatttt gataaggagt tgatccctga actgaatcga cttccgttag ccaatatcaa
    53221 acaattagaa gaagaattgg atagaacggg agtaacttcc caaaattccg agattgctaa
    53281 ttgagacatt ggcgcgatga aacggtgggt ctatatagcg ctatttttaa ttatcagaac
    53341 aagtgatgtt tgatcttcaa aataacaaat aaaaaattaa aaaatatagt tgacgaaatt
    53401 caaaaattcg attagactta aatcaattct taaggaaagg gtgacctgta atgaacttat
    53461 caaaacgtat ttcagcagaa aacaaaaaaa attggcgcag ctggcgtaga taagttgtat
    53521 taatggtgag actttcccat agatacaact ttttagagtg catttctaaa gcttgtatct
    53581 atgctagaca tgaacattga ctgccgcata gattagaatc tatgcggttt tttgtgtaaa
    53641 gacaatgacc gctttggcca ttgtcttttt ttgtagactg gatacttgcc tgattataat
//
LOCUS       DACEQH010000011        53371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.011, whole genome shotgun sequence.
ACCESSION   DACEQH010000011 DACEQH010000000
VERSION     DACEQH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.011"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(438..611)
                     /locus_tag="IW833_000670"
     CDS             complement(438..611)
                     /locus_tag="IW833_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HAQ1306807.1"
                     /translation="MNRYVHGTVQKKNRKINGKLLVNPNGYEWLRAKGSYPVPKYCNY
                     SETLMEWYQLIIT"
     gene            complement(954..1643)
                     /locus_tag="IW833_000671"
     CDS             complement(954..1643)
                     /locus_tag="IW833_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1306808.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFSHTRVGKNGKTFKMHKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYIGHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(1706..2470)
                     /locus_tag="IW833_000672"
     CDS             complement(1706..2470)
                     /locus_tag="IW833_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ1306809.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIVKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            complement(2498..3196)
                     /locus_tag="IW833_000673"
     CDS             complement(2498..3196)
                     /locus_tag="IW833_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ1306810.1"
                     /translation="MARTQKQKAAQTHVVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTIAANIAVVFAKSGQRVLLVDADLRKPVVYETFQLNNAS
                     GLSTALSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYRN"
     gene            complement(3208..3987)
                     /locus_tag="IW833_000674"
     CDS             complement(3208..3987)
                     /locus_tag="IW833_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ1306811.1"
                     /translation="MEETISLKEIFGTLRKHLATIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSENSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4003..4947)
                     /locus_tag="IW833_000675"
     CDS             complement(4003..4947)
                     /locus_tag="IW833_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1306812.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4993..5772)
                     /locus_tag="IW833_000676"
     CDS             complement(4993..5772)
                     /locus_tag="IW833_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306813.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKERVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISVAYATKAQTVAQQMGYSNINY"
     gene            complement(6157..8232)
                     /locus_tag="IW833_000677"
     CDS             complement(6157..8232)
                     /locus_tag="IW833_000677"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ1306814.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDYEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTYHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDADRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(8234..9151)
                     /gene="glyQ"
                     /locus_tag="IW833_000678"
     CDS             complement(8234..9151)
                     /gene="glyQ"
                     /locus_tag="IW833_000678"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1306815.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(9537..10349)
                     /gene="recO"
                     /locus_tag="IW833_000679"
     CDS             complement(9537..10349)
                     /gene="recO"
                     /locus_tag="IW833_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ1306816.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKDMSEKDLPKKEE"
     gene            complement(10492..11391)
                     /locus_tag="IW833_000680"
     CDS             complement(10492..11391)
                     /locus_tag="IW833_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ1306817.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(11406..11807)
                     /locus_tag="IW833_000681"
     CDS             complement(11406..11807)
                     /locus_tag="IW833_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ1306818.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIVGLILFVPKIYHLLFN"
     gene            complement(11785..12261)
                     /gene="ybeY"
                     /locus_tag="IW833_000682"
     CDS             complement(11785..12261)
                     /gene="ybeY"
                     /locus_tag="IW833_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ1306819.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(12290..14482)
                     /locus_tag="IW833_000683"
     CDS             complement(12290..14482)
                     /locus_tag="IW833_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ1306820.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLFAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(14506..15477)
                     /locus_tag="IW833_000684"
     CDS             complement(14506..15477)
                     /locus_tag="IW833_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ1306821.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            16183..17562
                     /gene="brnQ"
                     /locus_tag="IW833_000685"
     CDS             16183..17562
                     /gene="brnQ"
                     /locus_tag="IW833_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1306822.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGTNWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(17718..18164)
                     /locus_tag="IW833_000686"
     CDS             complement(17718..18164)
                     /locus_tag="IW833_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ1306823.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(18191..18367)
                     /locus_tag="IW833_000687"
     CDS             complement(18191..18367)
                     /locus_tag="IW833_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ1306824.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(18529..18957)
                     /locus_tag="IW833_000688"
     CDS             complement(18529..18957)
                     /locus_tag="IW833_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1306825.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            19049..19909
                     /locus_tag="IW833_000689"
     CDS             19049..19909
                     /locus_tag="IW833_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ1306826.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(20004..20297)
                     /locus_tag="IW833_000690"
     CDS             complement(20004..20297)
                     /locus_tag="IW833_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1306827.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(20370..22097)
                     /locus_tag="IW833_000691"
     CDS             complement(20370..22097)
                     /locus_tag="IW833_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1306828.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            complement(22311..23237)
                     /gene="trxB"
                     /locus_tag="IW833_000692"
     CDS             complement(22311..23237)
                     /gene="trxB"
                     /locus_tag="IW833_000692"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ1306829.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(23382..24815)
                     /gene="ascB"
                     /locus_tag="IW833_000693"
     CDS             complement(23382..24815)
                     /gene="ascB"
                     /locus_tag="IW833_000693"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1306830.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(24815..26218)
                     /locus_tag="IW833_000694"
     CDS             complement(24815..26218)
                     /locus_tag="IW833_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1306831.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            26448..27350
                     /locus_tag="IW833_000695"
     CDS             26448..27350
                     /locus_tag="IW833_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1306832.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(27391..29277)
                     /locus_tag="IW833_000696"
     CDS             complement(27391..29277)
                     /locus_tag="IW833_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1306833.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIRAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILMMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGNLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKTSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFKSL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(29373..30224)
                     /locus_tag="IW833_000697"
     CDS             complement(29373..30224)
                     /locus_tag="IW833_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1306834.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSEQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGEKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(30681..32465)
                     /locus_tag="IW833_000698"
     CDS             complement(30681..32465)
                     /locus_tag="IW833_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1306835.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQTITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(32465..34216)
                     /locus_tag="IW833_000699"
     CDS             complement(32465..34216)
                     /locus_tag="IW833_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1306836.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFVIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(34358..34582)
                     /locus_tag="IW833_000700"
     CDS             complement(34358..34582)
                     /locus_tag="IW833_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ1306837.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            34833..36419
                     /locus_tag="IW833_000701"
     CDS             34833..36419
                     /locus_tag="IW833_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ1306838.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            complement(36729..38504)
                     /locus_tag="IW833_000702"
     CDS             complement(36729..38504)
                     /locus_tag="IW833_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ1306839.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSRRKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(38715..39617)
                     /locus_tag="IW833_000703"
     CDS             complement(38715..39617)
                     /locus_tag="IW833_000703"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ1306840.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEGGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQAFDPEVFPELLAEEVTL"
     gene            39896..42241
                     /locus_tag="IW833_000704"
     CDS             39896..42241
                     /locus_tag="IW833_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1306841.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKVIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            complement(42404..42670)
                     /locus_tag="IW833_000705"
     CDS             complement(42404..42670)
                     /locus_tag="IW833_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306842.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNHQ
                     MTAEEAAQEFSNRHVHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(42663..42986)
                     /locus_tag="IW833_000706"
     CDS             complement(42663..42986)
                     /locus_tag="IW833_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306843.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            complement(42998..43204)
                     /locus_tag="IW833_000707"
     CDS             complement(42998..43204)
                     /locus_tag="IW833_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306844.1"
                     /translation="MTEQEIIEALASVVATKENLVDSAKEVYLLRINKARRMGEAFDT
                     LVKEIQDKINEIVTRDRELAQQFN"
     gene            complement(43201..44256)
                     /locus_tag="IW833_000708"
     CDS             complement(43201..44256)
                     /locus_tag="IW833_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ1306845.1"
                     /translation="MMADKQYSVISEAVYWLDPKHRDYDHTYKENSIKKIDSYNFKIL
                     KIKDSLDGMQAMGVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRPG
                     ASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALYV
                     AAEKQWKNVGLNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEVR
                     ISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQEL
                     NNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELEK
                     KWHGKKACGKHVLSPLR"
     gene            complement(44256..44651)
                     /locus_tag="IW833_000709"
     CDS             complement(44256..44651)
                     /locus_tag="IW833_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306846.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGAYRMFVTMINNHDESVYFTFGFWKERNELGDY
                     GVENKSIQKEGITVNKIRVIFSNNKEEYI"
     gene            complement(44694..44948)
                     /locus_tag="IW833_000710"
     CDS             complement(44694..44948)
                     /locus_tag="IW833_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306847.1"
                     /translation="MTVKDEEYRYISEHVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKDSLDGMQAMAAAPIVHSKLEKNFKNKKIPTNFRVLK"
     gene            complement(44945..45340)
                     /locus_tag="IW833_000711"
     CDS             complement(44945..45340)
                     /locus_tag="IW833_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306848.1"
                     /translation="MPKKRRKTIVIILTILAIGIGGKFYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGCYGALVTMTNNEGKSVYFDYFFSKQMNEISSY
                     GVENEEIQNEGVTTPKIRVIYSNGEAEEV"
     gene            complement(45694..46695)
                     /gene="ccpA"
                     /locus_tag="IW833_000712"
     CDS             complement(45694..46695)
                     /gene="ccpA"
                     /locus_tag="IW833_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ1306849.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            46905..48008
                     /locus_tag="IW833_000713"
     CDS             46905..48008
                     /locus_tag="IW833_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1306850.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGADSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVVWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            complement(48070..48672)
                     /locus_tag="IW833_000714"
     CDS             complement(48070..48672)
                     /locus_tag="IW833_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1306851.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSAELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(48675..49115)
                     /locus_tag="IW833_000715"
     CDS             complement(48675..49115)
                     /locus_tag="IW833_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ1306852.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIIRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            complement(49241..50179)
                     /gene="galU"
                     /locus_tag="IW833_000716"
     CDS             complement(49241..50179)
                     /gene="galU"
                     /locus_tag="IW833_000716"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1306853.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKIELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            complement(50194..51219)
                     /locus_tag="IW833_000717"
     CDS             complement(50194..51219)
                     /locus_tag="IW833_000717"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1306854.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNHYFRIYTNPDVVGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            complement(51263..52093)
                     /locus_tag="IW833_000718"
     CDS             complement(51263..52093)
                     /locus_tag="IW833_000718"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1306855.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLLVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            complement(52108..53046)
                     /locus_tag="IW833_000719"
     CDS             complement(52108..53046)
                     /locus_tag="IW833_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ1306856.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            complement(53183..>53371)
                     /locus_tag="IW833_000720"
     CDS             complement(53183..>53371)
                     /locus_tag="IW833_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1306857.1"
                     /translation="NSNGILRRNGLPKSMDFREVNQTFISSVSNQRNYIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT        14870 a        11317 c         9454 g        17730 t
ORIGIN      
        1 tatcttaatt atctatccat ttcatcctac gcattcttgc gtaaatacaa ttcactctgt
       61 tgagctgata taaatcattg gtggtacaaa gataaaatga taactgtttc tatacctcca
      121 aaaattccag taaatatatc tatagatagt ttcaaaaaaa attgtcttag tttcataata
      181 tttaagatag tggtacatac tttataacaa cgtacttttc caaatattat tttccgttat
      241 gaacatccaa tatattacaa agtttaaaag tttagatgaa ctataatctt catttctgca
      301 cagtggataa taaattctta tagataaaaa atagcctctt ctaaagtcac aaatacctta
      361 ctcccacatt ttaagtaaac actctgcagc attctctttg ttataaaatg atagatattc
      421 ctcaatttta tctcttatta tgttattatc agttgatacc attccattaa tgtttcagaa
      481 taattacaat attttgggac cggataactc ccttttgctc gtaaccactc atatccgtta
      541 ggattcacta aaagtttacc gtttattttt cgattctttt tttgaaccgt cccatgaaca
      601 taccgattca acaagctaaa ttataaaaat tggtgtgctg tcattatttt ttggaaaatt
      661 tattaatggc tatcaatata cttttgtatc atactaaata gctcgtcagt tcctatatcg
      721 gatataccaa tattaaagca tttagatcat tatatttaag agtggtatca tcccaaaatt
      781 tcgaatattt tcttttatat aagaataatg atactgaatt gccccattca cctttctttt
      841 tttagatttt ctacaagtga ttcaaaccct ccataattac agttattact tttgaaacga
      901 taatatgaac atgtgtaatc ttttttatta acaatccatc attctatccc taactaatat
      961 gctccattgg gcttgacaat aacataaaac gtttttaaaa tcaacctcaa gtcgtacatt
     1021 aaacttcgat gaccgatata ctctaaatcc aactcaacca ttttatggaa actaacatta
     1081 tttctatcac taacttgcca caaacctgaa caaccaggtt ttacatacaa gcgttgaaga
     1141 tcatatctcg tgtactcagc tacttccctc tccaaaggtg gccgtggacc aattagactc
     1201 atatcgcctt ttaaaacatt ccatagctgt ggcaattcat caatgctttt ggcacggata
     1261 attttaccta ttttagtgac acgcggatct tctttcatct tgaacattgc accttctatt
     1321 tcattttgat caagtaattc ttccaatttt tcttcagcat ctaaacacat cgaacggaat
     1381 ttatgcattt tgaatgtttt tccattttta ccaacacgtg tatggctaaa aaatattgga
     1441 cttcctggct cttcttttct gattcgataa gctacataga acatcaaagg agaagctaaa
     1501 atcaaaccaa taatgcttcc tacaatatca atcattcttt taaaaaataa atagatatat
     1561 cgtttttcaa ctttttcttt atccaaccaa acttgattag gatgtacttc actaatacga
     1621 gataataaat cttcactttc catactttca gtccctttca ctatcttcac tctctcctca
     1681 tttgttttta tctacgaata aagaactaaa ataaaccaaa cttcttcttt tttacctctt
     1741 catagattgg ataactgatc tcatccccgt tgatcagatc ctttgcgact tgtttcatct
     1801 gtactacttt ttccttacca aagtccttga caatctgctc atagcactct tttaagtaaa
     1861 aggtgcgctt agagaccccg tgtgcatcag aagcagccat ctgcacgaga ttatgtttga
     1921 ccattgtttt ggctgttttc tggatgtctt tgccaaatac accaacataa cttggcgcag
     1981 taatttgggc caaacatccc atatctagga acggaagcaa atgatttggg tcttctcgaa
     2041 aatgcgcgtt gcgttctgga tggacgatca ctggtgtctt tcctagttct aacaactcaa
     2101 aaaaaagcca ttctgcatat aacggcactt ccagtgtcgg aaattcgatc agaagatatg
     2161 tatcatctaa atctgtaaat aaaatttcat ctcggtggat gtcttcgatg agcgttccgg
     2221 taatacgtac ttcttgtcct tccaataacg tcaaaggcaa ctgtcttttc tctaattcgg
     2281 cttgtaacga agcgactaga gaaatgactt gtgatttttc attgctgtat tttccattat
     2341 tgtggtgtgg tgtgcataaa atatgcgtga tgccttgctg aatcgctttt tcagccattg
     2401 caatacttgc ttctaagttt tctgccccgt catcgattcc tggtaggata tggcaatgta
     2461 gatcgatcat ttctcttgtc tcctctcttc ttattcctta atttctgtag taataaccag
     2521 catctttgct atgttctgct ccgttgtata caacacctaa gactcgtgct tgtaccatct
     2581 ctagcagttc ttttgcttta gtcaaggatt ctttacgtga tgtattttca cgtacgacca
     2641 ataaagtgcc atccgttttt gaagacatga tctgtgcatc tgttacggct actactggtg
     2701 gcatgtcgaa gatcactaca tcaaatagtt gtcttgcttc tgctaaaatc tgatccattc
     2761 ttggcgagct taataactct gatggatttg gtggtttagg tccacttggc agaatcgaca
     2821 aattctcgac tggtgttcgt tggatctcat ctgctacact tcctgatgaa ctaagtgctg
     2881 tacttaatcc actggcattg ttcagctgga atgtctcata gacgacgggt ttacgtaaat
     2941 ctgcatcgac tagtagaact cgttgaccgg atttagcaaa aacgactgca atgtttgctg
     3001 caatggtgga ttttccttct ccaggacctg atgaagtcac gacaatcgtt ttgatttgct
     3061 gaccagctgc ggaggcaaac tggatattcg tccggatcgt acgatattgt tcggcgattg
     3121 gtgaagatgg gtttgttaat gtgatcaaac tgactacatg agtttgtgct gctttttgtt
     3181 tctgtgttcg tgccattttt cttcctccta gactcttgag cggcttctgc gtgatggagc
     3241 tgttttatcg tttgtagctt ttggtcgtgc aggagctgat ccttttttac ggataccagc
     3301 gttcaattct ttcgctgaca tttctggaac tgttcctaag attgggaatc ctaatgtatc
     3361 cgtcacatat ttttcatctt tcactgtccg atcaagtaat tcgaataaga atgccaaacc
     3421 tacgcctacc atcattccta atactaagcc aatcactaga ttcaatttat tatttggcga
     3481 aacaggtgtc gtattggcaa ctgcacctga gatgatcgag attttatcga cattcatcac
     3541 gtcttttgcg ttctcttgga aaacttgtga agttgtatta gcgatctgtt gagcagtcac
     3601 tgcattcgtg cttattgcct ggatcgagaa catttgtgag ttttctgact gattgacgga
     3661 gatcgcatct ttgatttgtc cagcggtcat tttcacattg tcttctgttt ctagacgatc
     3721 tttgacttca tttaatacta gatcactaac gatcatatct ttatacgtat tgatcatttg
     3781 caggttcgta ttgacatcat tgacattcgc tgtgtctgat tgcggcagtg tcacgatcaa
     3841 ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat gttacgattc ctgagatggc
     3901 caagccagca aacattgaga tgatgattgt tgccaagtgt ttgcgtaatg ttccaaaaat
     3961 ttctttcaag ctgattgtct cttccatttt ttctccactt tcctatttat tctctagatt
     4021 catttgatct tttaattctt ttttcacacg atcaagttct tgatcgctga ctttttgata
     4081 ggagatatta tcttgcatga agccttctcc ttgcagctga tcttgcttga tcgtaccaaa
     4141 tgcctcccga taatctaaag cgattttttt catttcatca aagctcatgt ctgttttcat
     4201 attcgattct agagcattca aaacggattg atagttgctg acaccgttta gactcaatac
     4261 ttttttcgct acaccttcta cgatctttct ttgacgctct tgtctaccat agtcaccatt
     4321 tggatcttca taacgcatac gagaataaga taatgcctga tccccgttca atgtgatttt
     4381 accgattgta aagtcatagc catcttgtga gaaagtgagg tcattattga cttcgatacc
     4441 accaactgca tcaacaagtt cttttagccc tttcatattg atagagacat agtgatcgat
     4501 tgggatgtct acatattttt gtacggtatc catcgccatg gcagctccgc cgaatgcgta
     4561 agcgtggttg atcttgtctt ttgtgtcacg acctacgatg tcgacatacg tatctcgagg
     4621 aatgctgacg atcgttgtct gtttgtcttc aggactgact gtggcaacca tcatcgtatc
     4681 tgatcttcct tgatcgaccc gacctaaatc tccagtatca atccccatca atagaacaga
     4741 gaaagattct tgtttgtcca gatcaactgg tttcgttcgt tctccttggg ttcgttccac
     4801 tgattcgtaa gttttctcaa tagaatgtga gagatccaga tataattttg ctcctactcc
     4861 tacaattgct aatactaata cgataagaac tgctaaaata atcagaaata tttttttccc
     4921 tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt gcttgaatca
     4981 tcgctttaac tgtcaatagt ttatattgct atagcccatt tgctgagcaa cagtttgtgc
     5041 ttttgttgcg tatgctacgc ttattttttc attccggatc tgctctattt gactgagcgc
     5101 cgtcaaatat tgatcgtaag tagcggtggc agtcacttga ccatccttta acatcgtatc
     5161 gaataactga tctagttgcg tgacttgcgc gatctgggta tcagcgaatc ctagatattg
     5221 cataacaacc gttttccaag gactatcttt aaaaaagctc atattttcac gtacacgagc
     5281 gacatctgca cttgtcaccc tttctttgat gatgacatcg tctacagctt gctgccagtt
     5341 tgatacattt ttagtaaata aatcgttgac agctgtctgg atcttcagtt ttgattctgc
     5401 actatctaac tgtgtcacga ttgctttctt ctcatcttgg attgcaacag cttctttagg
     5461 caattctgat tctttgatgt cgtaatcatc tgctgaaagt ttgacagagg atagcttatt
     5521 tgacaaactt gtgattgttt cttctgttat ttcagggttt agaaaaattt tttcttttgt
     5581 actatacagc ttgtctactt cttttgataa agaagtcaag gtgtcttttt gattggtgat
     5641 ggcagattct gcataattga tccgttgctg atgttgccgc tgattattga tatggtatcc
     5701 aaacaaacag atcgcaatag cacctgcacc taccgccaca gcagtcaatg acttgacgaa
     5761 tggcttttcc atagattcgc tcctaagtat tgatgataga taaattaaga tataattcac
     5821 ttacataaaa atgacagttt tttatttaaa aaaacataat taaactaata tattcacaga
     5881 aatatttgta aacaagtttt ccctatataa tcataccaca aactgctttc ttgactaggg
     5941 aaatttacga tttaaaagta aataaaacat taataagata acaattaatc gattttactt
     6001 ttatttcctt aattttcata ggtgttttta aaagttacac aaaaaataaa ataacaaaaa
     6061 aaattaacat tgaattaata atagttattt ataacaaata aaccagagaa atagcctatt
     6121 ttgtgcctgt tctctggttt atttacatat tagaaattat tttgtgatga tttgatctaa
     6181 acttgctagt gacaaagcca tggcagcaat ttgacttaat tgcgttaatc ggttcttacg
     6241 tactgctggt tcttctgcca tgaccatcgt ctgatcaaaa taattttcga tcagtgggcg
     6301 aaggcttaca agctgatcat aattttcgtt gatcgtttgg tcagcaaagc ttgttttcat
     6361 tgcctctact gctttgtgca gttctttctc cgcttcgttt tcgaacagtt cagggtctac
     6421 ttctgattgt ttttcaagct ccgctttttt cgctaagttc acgacacgtg tcaaagcttc
     6481 catcgatggt ttgaagtctt catctgcaag atgctgcttg aacaattgtg ctgctgcaaa
     6541 tacttttgtc agatcttttt gttctgcatt caaaacagct tcgatgacat catgacgaat
     6601 attcttcgtt gtcaataatt ggcgcagacg gcctttgata aagtcgatca cctgttgttg
     6661 tcccgctgac aagccaatgc caaaacgatc cgcatcttta ctgataactt cactaatcgt
     6721 ttcttgcaga acagataaag gaaaggccca ttttttcgat tcgatgattc gaacgacacc
     6781 atatgcttga cggcgcaaag catatggatc gtttgagcca cttgggatca tcccaactgc
     6841 aaagaaagaa aagatactat ctaatttatc agcaatcgct aaaacagaac ctactgtagt
     6901 ctcaggtaga tctccttcac tggatatagg catgtaatgt tcacggattg ctgttgctac
     6961 ggctggtttc tcgcctttaa gcagagcata tttttctccc atgatccctt gtagttcagg
     7021 gaattctcct accatgttgg ttactagatc aaatttatag atttcagcag cgcgttgcag
     7081 atccatcaat tcttcctcag atagtcctac ttgctcgcct atgatgcggg cgatgacttg
     7141 tacccgttgc attttctcgt agatgctacc gattttttca tggtaagtaa cagtttttaa
     7201 tttttcaacg tattggtcga ttgaagcttt cttgtcttca ttataaaaga attccgcatc
     7261 ttctaaacga gcagtcaata ctttttgatt gcctcgaacc acattttcca attttacccg
     7321 atcaccatta cgagcagaaa taaaatgagg aagcagcata ccttgatcat tccgtacctc
     7381 gaaataacgt tgatgttctt tcatagaagt gactagtact tcttcaggaa cagaaagata
     7441 tttttcttcg tagtcgccag aaaagacagt tggatattcg actaaattgt tgacttcttc
     7501 tagtaaatct tcgtctagat caataaccca gtgatttttt tctgctaatt cgtttgcttg
     7561 tgtaacgatc atttcttgtc ttttttgcgg agacgcaatg acgaattgtt cattcaattt
     7621 ttcttcatat tcttttgcat gcgcaaatgt cacatcttcg cctaagaaac gatgacctcg
     7681 agaggctctg cctgttttga catccaagat ttcgaatggg attacttcat catctaacaa
     7741 tgccacgatc cagtgaatag ggcggatata ttcaaaatcg tattctgccc aatgcatcgt
     7801 tacagggaaa gtcaggcttt taatgacatc tgctaaacct gtcaatactt ctttggattc
     7861 cttaccatga gtgtatttcg ttacatagac atattccaca ccttttaatt ctctaaaagt
     7921 gatagcgtct gtcgtcagac cttgtccgcg gacaaatcct tccgctgctt tcgtccagtt
     7981 tccatctgcg tccaaagcaa tttttttggc tggtcctttg acttcttctt cactgtccgc
     8041 ttgtcgttct ggaatatttg ttactttgat tgctaaacgg cgcggtgtgg aaaatgtttc
     8101 gatcgtatca taagtcaatt ggttttcatc taaaaatttt gctgtttttt cttctaattg
     8161 tttcatgctt ggtgtgacaa catgggcagg catttcttcc agcccgattt caagtaataa
     8221 gtcttttgcc atcttattca gcctccttca cggtttctgg atttgctgtg tttttattca
     8281 acaaagggaa tcctaatttt tcgcgttctg ccacaaagat tttggctaca gcacgagcca
     8341 tgttgcggat acgggaaaga taaccggcac gctctgttac ggatactgca ccgcgagcat
     8401 ctaataaatt gaatgtatgg ctgcatttca aaatataatc atacgctgga tgaacaagat
     8461 tttcatcgat acagcgttta gcttcttttt caaaacgatc aaagttgctt agtagcaagt
     8521 cttgatcgct tacttcaaaa gaatatttgg aatgttcata ttctggttgt ttgaagatct
     8581 cgccatattt cacgccatca gcccattcta aatcatagac actttcaact tcttggatat
     8641 aagaagcaag acgttctagt ccataagtga tctcagaagt gactggttta caaggcagtc
     8701 caccaacttg ctggaagtac gtgaattgtg tgatttccat tccgtcaagc catacttccc
     8761 aacccacacc ggcacagccc atggatgggt tctcccagtt gtcttcaaca aaacggatgt
     8821 cgtgttctaa tggatcgatt cctaaaaggc gcaagctttc taaataaagc tcttggatat
     8881 tctcaggtga aggtttcatc acgacttgga attgatgatg ctgatacaaa cggtttgggt
     8941 tttcaccata acgtccatca gctggtcggc gagatggttc tacgtaagca gcattccatg
     9001 gttctggtcc gattgcccgt aaaaaggtat atgggctcat cgtacctgct cctttttctg
     9061 tgtcatacgc ttgcatcagc atgcagccgt ttgatgacca aaatttttgt aatgttaaaa
     9121 tcatatcttg gacggtcaat tttttactca tctgttttgc ccctttcaat tttaaagcgt
     9181 tgctgtcaaa taagaataca ggaaagaaaa cgacaaaaaa tccctatgtc tgattacaga
     9241 catagggacg aataattcgc ggttccaccc tacttccgaa taaagattcg gcagcttcat
     9301 ttatcgtact cgcaagtgcc tcttcaaaaa tcgtttgggt ccggctccca ctctcccgaa
     9361 ctcgctaaac cacgtgtttt ttactgatct tggtcaatgt acgtattcat tttgcagttt
     9421 tcttgtttct ataatagatc ctctcactga acgatctatt tgatctacgg ttaataatag
     9481 acggaactcc ctgtgtttgt caaatggttt tgattttttg ttgtatgttt catttactat
     9541 tcttcttttt taggtagatc tttttcagac atgtctttct ctggcgtctt taatacgtgt
     9601 gcccaagact tcatttgatc gatgaacttt ttgcttttca aatgaatacc gacatattca
     9661 tcatacagct ggtcgatgag ttcacggatt gcttgtttgg tttcttcttt cagatggatt
     9721 ccactgattt tatcataaga aatagctgaa aacatccgaa cgaaataaac agctcgagga
     9781 tctgcatgat aacggtgatc gtccatcgac caatgtcttt cgcatatcac accaccatat
     9841 ttggaagaat agtcaaattt tccttttgtt tccccacata cgacgcagtg tgtccagatt
     9901 ggtgcaattc cgaagcgttg cagtatttga acttcaaaga tattcgtgat gatttctgca
     9961 tcatctcctt gatccatccg ctgcagagct tgttgcgtaa attggaataa atgcgggtca
    10021 tattgatgat cttcgattgc tgcatctact agatttagca aataggtacc ataaccgctc
    10081 aaaaagatat cttcttggat gttccgaaac gcatggacgt ctttgacact gcttaaaaac
    10141 gacaaacctt cacttcgaaa attaccgata tagactgctt cggtaaaggg gagcaaagcc
    10201 ggagctaatg gctgattttg ccgatggatt cctttgatat aaaacatcat tttgccagaa
    10261 gattccgtaa agattttgac taatttatct ttttctttgt aattacggct agataaaatc
    10321 aagcctttcg attctgcaag ctggctcata ttctccctcc taaaaaataa atctggaaaa
    10381 gcagattctg ccgatccaga ttatttatgt ttcgatcttc cgccgttttt ctcaacgtgc
    10441 ggatttttat ccagtttatt tctatactta acctataaga agttcagaat atcagtattc
    10501 atcttttcgg tagccgaagt cttgcagata gacttttttg tctcgccagt ctttttggac
    10561 tttgacccaa agttctagaa atactttgtc tcctaaaaga ttttcgatat cctttcttgc
    10621 tttcgttcct acttgcttca gcatttttcc gcctttgccg ataatgattc ctttttggct
    10681 atctctttcg acgatgatcg tggcctggat atggatttta tcattctcgt ttcgcttcat
    10741 cgaatcaaca acgactgcga cagaatgagg cacttcgtct cgtgttaaaa acaagacttt
    10801 ctcacggatc aattcagaaa cgatgaagta ttcaggatga tcagtcactt gatcatctgg
    10861 aaaatattgt gggccctctg gcatttcact gactaataca tccatcaaac gttcgacgtt
    10921 gtttccttct gtagcagaaa ttggaatcac ttgtgcaaag ggcatctgcg ttgaataatc
    10981 ttcgatgatt cccaacaact cgtctggatg gattttatcg attttattga taatcagata
    11041 aacaggagaa tgcacgtttt ttagacgttc aataataaaa tcgtctcccc ggccgcgttt
    11101 ttgatcagcg ctgatcataa ataagaccgc gtccacttct ttcaacgcac tgtaagctgc
    11161 ttctaccata aaatctccta aacgatgctt tggtttatgg attcctggtg tgtcaataaa
    11221 aatcaactga gcttctggtg tagtatatac tccttggatt ttatttctag ttgtctgcgc
    11281 cttatcactc atgatggcaa ttttttgacc cacgattcga ttcaataatg tggattttcc
    11341 tacattcggg cggccgacga ttgcgacaaa ccctgattta tgttctggca tatacgttct
    11401 cctttttaat taaacaacaa atgatatatt ttagggacaa ataaaatcaa cccaacgatg
    11461 acggcaaaac aagcggtcac taaaactgct ccagctgcca tatctttgat ttttttccca
    11521 atcggatgaa aatgaaaatc cgtaaacatg tctacgacat tttcaaaaac agtattgatg
    11581 atctctacta tccatacgag aaaaacagct aaaagcagcc ataaccattc tatccgatca
    11641 agttggaaaa tcacgcccat aaacaaagcg aggattccga acgcaacatg tttcctcatg
    11701 ttgcgttctt cctcaaaaac agttttgact ccttggacgg caaattccac ggacatcagg
    11761 aaatgtttgt ttttctctac gtttttatct tttaagtcca taggcatcta agatctcttt
    11821 ctgtaaacca aacatttctt tctcatcctc tggtgtcata tggtcatacc cgttgatatg
    11881 caagaatcca tgaaccgcta aaaagccaag ttcccgatca tacgaatggc cgtaagcttc
    11941 tgcttgctct gcagcacgtt cagtagagat catgatgtct cccagtacac gaggcaatgg
    12001 attctctcca tcttcaaaaa tcactgggat ttcgtcttca ccctcttctt ccaaggcaaa
    12061 actgatgacg tctgtcggtg catcttttcc acggtaatca cgattgatta cttggatcgc
    12121 tgcgttgtcc atgaaagtaa cagacatttc tgtttcttct ggcaactcta aatatgatgc
    12181 tgcaaattgc aacaagtcgt cgatttcttt cattttctct tctgagactt ggtttgtttc
    12241 atccatgaag gtaatatcca ttatacatcg cttctttcta ttcttcttgc tattcttctt
    12301 gttcttcttt tatcttctca acttccgatt tcattttagg atatttgatc cgagaatgga
    12361 aggtactgct taaaccgctg atcaatgatt tttctacttt acggatttct tttaacgtca
    12421 atccactgtc atccaattgt ccatcggaaa tacgctcttc tataagatta tggacaaaac
    12481 tttcgatttt ttcgcttgtt ggatggtcca ttgcccgtac tgccgcttca caactgtcag
    12541 ctatattgac gattcctgct tcacggcttt gaggttttgg tccaggataa cggaattgct
    12601 cttccgtgac ttccgggttg cgttcctttg ccttaacgta gaaataacgc atcagcgtgg
    12661 ttccatgatg ctgtctgcaa atatcgatca ccatttgcgg catattataa tcttccaata
    12721 ttttggcacc atctgtcaca tgtccaaaga taatatgttt actgtcttct ggcaataaga
    12781 aattgtgcgg attttccgct cctgaaggca agttctcaac gaaaaagctg gcgtgtttga
    12841 tcttgccgat atcatgataa tagcaagcta ctcgagttag taaagaacgt ccaccgattt
    12901 ctgctacagc attagcactt agattggcta ccatcatgct atgatgatac gtacctggtg
    12961 cttcttctaa caactgtttc aataaagggt gattaggatt gctgagttca tttaatacga
    13021 tcacactatc atcattgacc atcaattcaa tataggggtg cagacctatc gtcagcaaga
    13081 atgagaacaa tgcacctgtg aatccgcaga ctagcgttaa ccaagtcgtt gcatccccaa
    13141 agctcattcc ttggtagata atcaaaatga catccatcat cacagggaaa ataatgaccc
    13201 acatagcagc gaataaccct tgttctgaga tccgtttgcg tctgacgatc gttgccatga
    13261 gaccagaaaa tacataggac atcaaaataa ctgtcagaaa gttcgtgcca atcgaatcat
    13321 aaaaaatgaa aagtgcggat actgcttgga aaataccagc catgatccct gctcttcgat
    13381 tcaagaaaaa gcttaaaaca agtggagcaa aagccgcggg gtaaaacagc ggaatataag
    13441 cagcttgttc tgtttggaag agttggaaaa atttcattag tactacgctg actgacattg
    13501 ctgtcacgta gaaaagtacg tattttgttc tttctcctgc attttgaaat tgaagtgaat
    13561 tataaatcag tacaccgatt tgaagcaaaa cagctaaaac catggcaatg agtgggaaaa
    13621 tcgaagtcgt ttgattcgtc aatcctagta attccaattt tttcatcgct gaagcatcaa
    13681 tttgatttcc ttcgcgaaca atgatttctc cttggaagat catcactggt tgtacagctt
    13741 ctcgtgcaga ttgttttaat tcttctgtct ttttctcatt caatacatca ttgactacaa
    13801 tgccttgatc cactaagtag cggatcattt gttgctgctc tgttgtgaca ttcaaatact
    13861 gaatctgatc ttctgcttct tgtttaaaag cttccaattc gctttcacga acttgtttac
    13921 tcatctgttc gtcaatgagc ttcaaacttt catccctaac cgtgtgaatt tccgtttcag
    13981 tcatttcaaa tatcgtcgaa taaaaattgt tcggcagttt ttggtaaaac gcaacgtttt
    14041 ctgcattcac gttttcaaaa tttttcttca gtgtagcaat ccgctcttct gtagtaggtt
    14101 tcgggatatt ttcatcttcc ttcgccttat ctaccttgtc attataagat ttgttgatgg
    14161 tatcattcgt cttatcgatc aattcgaata attgcttgat ccgattgttc tggtcatctg
    14221 ccagatcttt ttgataagta tattctggag tcactgcttc tgctgctagt tttcttcttt
    14281 gttcggtttc ttccgtgttc tcgatcgttt tattcgcacg gatactctct tctgctactt
    14341 ggccttctct aaaatcattg gccttttgtt tgacactact aaatgtaacg ccgcaaacaa
    14401 tgataaaaaa caagaacaga aggaaaggca atgctgcctt tccgagtttt tcttgtagtt
    14461 tctgcagtgt tttattcaac atgtgcttat cctcctaagc aaagcttatt cttgatgaag
    14521 atccgccttt tcataagctt tgatgatttc ggcaactaca gggtgccgaa caacgtcact
    14581 tgcttcaaaa ttaacaaaat cgattttttt gatttgcttt agtgttcgtt ctgcatgaac
    14641 aagaccactt gtcgtccctc gaggcaagtc tatctgactt gtatccccat tgacaatcat
    14701 ttttgattga taaccgagac gggtcaagaa cattttcatc tgagcgactg tcgtgttttg
    14761 tgcttcgtcc aagataacga atgcatcatc caatgtccgc ccccgcatgt aagctaatgg
    14821 tgcgatttcg attacgcctc gttccatcaa tcggttcgta tgatccaatc caaaaatttg
    14881 gtataaagca tcataaacag gccgcagata aggatccact ttttctttca gatcccctgg
    14941 tagaaagcct aggttttccc ctgcctctac agcgggccgc gtcaaaatga ttttttgcac
    15001 ttcgcctttt tttaaggcag caattgctaa tactacggca agaaacgttt tccctgttcc
    15061 ggctggccct acaccaaata cgatgtcatg tttggctacc gattcaacat atttcttttg
    15121 accgctgttt tttacccgta taggttttcc attacggtct ttgacgatct cttcctcata
    15181 catctcaaca aaataatcta atgtaccttt ttgccccatt ttcaatgccg tcacaacatc
    15241 gggcgtactt acgtggattc ctcgtttgat caattcctgt aatgtccgaa tgactgaggc
    15301 agccaaggaa atctgggatt gttcaccaat gatctgaatg atttctccac gtgtatggat
    15361 ggttgtttcc gttgtttcct cgatcagttt tatatgcttg tcgtgtgcac ctaaaagcat
    15421 gctgacatca tcagcagctg tcaatctaat ctctaaagaa ctagactctt ccgtcaaaaa
    15481 tagacatccc ctttttttcg attcattctt tcctatttta ccatactcag aagaaactat
    15541 atagcaaata agaaaaggtt gtaaaagagt cgtttaacca tacgtaccaa gaaacgttaa
    15601 aaaatagccg tttccattcg ctaaaggtct cgttttgata aaaagaatct gagaatgtaa
    15661 gttatatgaa agtggagaac aaaatattcc gtttcattca gcaggtattc tctaggtatt
    15721 tttcttgttt caaaaaaaga aaacaaaata gttaaaatat tcttgcattt caaaaaaaac
    15781 aggtgtatta ttacgtcaac aaataaacat acagcaaagc gtcgaggaaa acagtaggct
    15841 attcaacgat ttattcccag tgagtcatgt aagtgagaaa tgacaataaa tcgaatagac
    15901 gaacatcatt tccgagtcaa tgtcgaaaac gtagatcatt gtggctgcct ccattatcag
    15961 gctagcggat aactttatca aaaagcgtcc gtgaatgaag aagctttttt aagcgaaaaa
    16021 aggtggtacc gttctagaat ctagagccct tttcccaagt ggaaaagggc tttttttata
    16081 ttttaaggtg gtggttttca tggaatctga tattaatggc attactaatt gctgcaacgt
    16141 ttatatcgtc aatcaaaatg ataattattg gaggaaaaat ttatggaaga aacaaaaact
    16201 tattcatgga aacaactatt gatggtcagt tcacttatct tcgggatgtt cttcgggtca
    16261 ggtaatctaa tctttcctgt ccacttaggt caaatggctg gcacaaactg ggtcagtgct
    16321 ggcgtaggat ttgccatctc aggcgctctt ttcccattac tagctatctt agctgtagta
    16381 gtgacaaaaa gtgatggtct gtatgactta gctaaacctg tcggaaaatg gtatgctgcc
    16441 ttgttcttaa tccttgttca tttgacgatc ggaccatttt tcggtacacc acggacagct
    16501 gctactgctt ttgaaatggc tgctaaacca tttttgcctg aaaaatataa ttcattaggt
    16561 atgtttttat tctctgctct tttcttttta cttgcttatt tccttactgt ccatcccaca
    16621 cggttggtca aatacgtcgg aaagtatcta aactccttgt ttcttgcatt attagcaatc
    16681 atttttgtcg tttctttatt ccacccaatg ggaaatctaa atcaatctgc tgcagatact
    16741 taccaaacaa acgctttaac aaatggtgtc ctagaaggtt ataacacggt tgatgcagta
    16801 gctttgcttg ccttaagtgt cacttttgtc catgcagtcc gtgaccttgg tttcaaagat
    16861 aaaaaagtca cagaattaac agcaaaagct ggtagcctga gcgttgcgct agaagtcatg
    16921 atctattttg gacttgttct tctcggtgcg atgagtttga ataagatgtc tttatcagaa
    16981 aatggcggaa ctgcactttc tcagattact aatttttatt taggtaactt cggcgctagc
    17041 ttcttaggag tgatggtcac attaggtgtc ttcactaccg ctatgggact cgttgtttct
    17101 tttgcacaag atttccataa actgttccct aaagtcagtt atatgacatg gctgcggtta
    17161 acgactttcg tctcttttgt cgttgcaaat gctggtttgg acaatatcat ccaatggtct
    17221 ttaccagtat tgatgctttt gtacccattg tcgcttgcgt tgatcttgct ttccttaaca
    17281 gcaaaatttt tccaaaagac accatttgtc tatcaagtaa cgatgttgtt tgctgctgtt
    17341 cccgccgtat tggatatgct ggcaaattcg cctgctttag tgagtcaaca aagagttgta
    17401 gcaagcatgc tcgaatttta tcatcatcac gttccttttg ctgcattagg acttggctgg
    17461 atggtcccaa cgttacttgg ttacgctggc agcttactct tctattacgc ctatcgccta
    17521 tcagggtaca aacaagaagc aaatgaacta ccagaagaat aaccagatca aacacaattt
    17581 aacataagaa atattaaaca ggaaaaaacc agctagatga aagaatccta ttctttcgtc
    17641 tagctatttt ttagatttct tttttacgag cacaaaaaaa ctgcggacaa tgattgtccg
    17701 cagtttaaat gataaaatta agattgtaat agttctttca caatggcatt tacttggttt
    17761 ccatctgctt ttcctttgac tttaggcatg atcgcaccca tgactttgcc aaattctttc
    17821 attgatgaag caccagtttg tgtgattgct tcttgaacaa gttgtcgaat ttcttcgtcg
    17881 gaaagttgtt ccggcatgta tttttcgact atcacaattt cactttttac tttttcagct
    17941 aaatcgtcac gtcctgcttc ttcaaactca tgcaaagaat cacgtcgctg tttcatttcg
    18001 cgagacaaca ccgttaattc ttcttcttct gtcaagtcgt gaccttcttt aatctgctcg
    18061 ttttgtatag atgacttgat cattcggatg acttgtaaag attccttatc tttcgctttc
    18121 atcgctgttt tcatgtcatc gtttaatcta cttagtagtg acacactacc aactccaatt
    18181 catcacagaa ctagaatttt ttgcgttttc tagctgcttc agattttttc ttgcgtctta
    18241 cacttggttt ttcgtagaat tcacgtttac gagattcttg taaagtaccc gcttttgaca
    18301 cggaacgttt gaagcgacga agagcatcgt caagtgattc gtttttgcga acgactgttt
    18361 ttgacatgtt aattccctcc ctccgagctt aaaaagtaac cgtaattgtt attgaaatat
    18421 atctcaaaaa acaagactgt cctgtaaaca ttatatctaa tgctgttttg accgtcaagg
    18481 aaatcttgct tttttttaaa attatcaaat gttattttat ttaaatcctt attgacaatt
    18541 ttcacatctt ccgaaaatct cgaatctatg cccttcgatc gtacatccac cgagctgttc
    18601 ttcgaaataa ttcattggac acatatggat ttctttcgtt gttccgcaca ccgtacagat
    18661 aaagtgatgg tgatggccga tttccccttg acagcaatga aaacgaaact tcatctctcc
    18721 atttaaatcg gtttcttcca gtaaatcgat ttctgcaaaa tcatggagat ttcgataaat
    18781 cgtatcgtaa cttactcctt tgaattcctg attcatatag tcaaaaactt ctttagcgga
    18841 tacgtagcga ttgcgcttca ctaaataagt taacaatgct tctctttttt tagtatattt
    18901 aaaaccgtta tttttcaatg tttctagtga ttgttcgatc aatgtctgtt ttttcatgac
    18961 ttattcctcc aaatcaaaat gattacgaag tacacattct agagtatggt ataataaaca
    19021 taaaaaaaat gcaagtgagg ttttatccat gtcttccgat tctacagaaa tcgttacttt
    19081 ttttgtcatc tccgactctg ctggagaaac tgcgacaaaa ttagcgcaag ccactatggc
    19141 ccaatatcct agcgttgagt tcaatttatt ccgcagaaca tttgtgacag atgagaaaac
    19201 attgatgcag gcactgcaag atgcactaga agaaaaagct cttgtcttac acacattgat
    19261 caatcaagaa ttgatcgaga tgacgcgttc tttttgtgaa gaacatcaat tatttagttt
    19321 tgatgtgatg actccgccga tatcagaaat cgagcgctta actggtatca agcccattcg
    19381 gcaacctggc gcccttcatt tattgaatga gaactatttc aaacggatta aagcaatgga
    19441 atttgctgta aaatacgatg acgggaaaga tcctcgtggg tttctagaag cagatgtcgt
    19501 tttgctaggc gtatcccgaa cttccaaaac gcctttgagc ttgtttttag ctaacaaaaa
    19561 tttaaaagtt gccaatcttc ctttgatccc acaagcacat attcccaaac agctttggga
    19621 gattgatcct aaaaaaatcg tgggattgac gaataaccca gatattttaa acaatatccg
    19681 taaagaacgg atgcgctctt acggattaaa tcctgatact gcttattctg atatcgaaaa
    19741 aatacgagct gaactcgatt ttgcaaatga tctttatgaa aaactgggct gcgtagtaat
    19801 caatgtcgct tctttatcta ttgaagaaac agcttcgctt attttgaatg ctttagacct
    19861 agaagatcat agttattatg gaactgaaac caccgaagaa acagactaac agtttttctt
    19921 gtctttttct taaaaaaaac tggactgtgt cagcgaagaa gattcgttga cacagtccag
    19981 tttgtctcta tttgcaccta tcgttattga tgttccacat gtttgagtgt ctggttcaaa
    20041 atatccagta cgtgagaatc atcaagacta tacaaaatcg cttttccctc acgatgggac
    20101 ttgacgacat ggttttctct taataaacgc agttgatgcg atacagcaga ttgttccatt
    20161 tccactgctt gggtgattgc tgtcacattt tgttccccgt ctttcagata cagcagaatt
    20221 ttgagacgag tggggtcact caacattttg aatagttggc tgactttctc tacttttgtt
    20281 ttttgcgtgt ctggcaatcc attcacctcc aacttgaaaa aagactgact tctaaagttt
    20341 accacttttt ccatcagtct caagtttttt tattcttctg tcatcttttg gataatcgct
    20401 tcataagcgg ctactttttc agctgcgtct tctgaagagt ttccttttac accgatgtaa
    20461 aatttgattt tcggctcagt tcctgaagga cggatagcga tccagccatt ttcttccagc
    20521 gtatacttca atacatcaga tggcggtgtc gttagttttt ctacgtttcc ttcacggtct
    20581 gttctcgtca actctttgaa gtcttctgtt tggatgactt ttgctccggc aaattctgtt
    20641 ggtgcttctt cacggaattt ttccatcaaa gcagagattt tcgcagaacc ttcttgtccg
    20701 ctcattgtca cagaaatcgt tttttctgcg tagtagccat aggtttcaaa aatcgtttgc
    20761 aagccgtcat acaacgtttt gccttgtttt ttatagtaag ctgctacttc tgctaataag
    20821 agcaatgctt ggatcgcgtc tttgtcgcga acgaaaggtt taactaaata accgtaactt
    20881 tcttcgaatc cgaacataaa agtatgggaa tgatcttctt caaattgttg aattttttca
    20941 gcaatgaatt tgaatcctgt caatacattg aacatggtag ccccatagtg ttccgcaatt
    21001 gctgtaggta attcgctaga aacgatcgat ttcaataccg ctgcgttttc aggtaaagtg
    21061 cctcgcgatt ggtgtgcttc caaaatatat tgagcaaaca gtgctccgat ttggtttccg
    21121 gtcaatactt cataggagcc atttggcaaa cgaacggctg cacctaaacg gtctgcatca
    21181 ggatctgttg caatcagcaa atcagcgcct tctttctcgc caagtcgtat cgcatactca
    21241 aaagcagagt gttcttctgg atttggtgat ttgactgtag tgaaatctgg gtctgcgaca
    21301 gcttgttctg gaactagtac gaattgttcg aatccagctt gtttcaacgc acgttcgccc
    21361 aacatttttc ctgttccgtg aagtggtgtg tacacaagtt tcaaatcttt ccccattgtt
    21421 tccaccaatt catgatcaat cgttactgct ttgatttctt ctagataagc ggcatccact
    21481 tcttctccga tgatcgtgat caatccgcta tgttcagctt cttcgtcaga taatacttca
    21541 acttttaacg gattgctgac ttcacggaca tatttcgtca atgcatctgc gtctgcagga
    21601 ggcatttgtc ccccatcttc gccgtagact ttatatccgt tgtaagctgc tggattgtgg
    21661 gatgcagtta ccatgattcc cgcaaacgtc ttcaaatgac ggactgcaaa agaaagctct
    21721 ggtgttggac gtagactttc gaatacataa gatgggatat tgtgatgtgc taatgttttg
    21781 gctgcttcca tggcaaattc tggtgattga tggcgtgagt cataagcgat agcgacacct
    21841 cggcgttttg tttcttcgcc ttgtgtatcc atgaaacgtg caagtccttc tgttgcttgt
    21901 cttaccgtat agatgttcat tcgattgatc ccaggcccta agattccccg catgcctgca
    21961 gtaccgaatt ccaacggtgc ataaaaagca tcttcaagtt tttcaggatc ttcacccaga
    22021 tcatttaatt gtttttttaa gttttcttct aaattttctt catgtaccca ttgttcatac
    22081 acttgttccc aagacatgaa aataacacct ctttctgaaa tatggttctt aataagtata
    22141 gcattcactt attagatata aaagtatgag gaccaaaaaa cgcttaaatc aagaaaggat
    22201 tcttttatca agcacggttg caagtattcg cataaatcgg attcagtaga tttcacgcga
    22261 aaaaaaagag taaaagaaca attgaaaccg ttcttccact ctttttaaac ttatgcagtt
    22321 gtttcttcta aagattccaa gtatttatac acttgttgtc cagcgatccc accatcacca
    22381 acagcagtag tgatttgtcg caaatctttt tcacgaacat ctccgatggc aaaaacgcct
    22441 ggtatattcg tcttcatttc gttgtctgtt ggaatccagc cagcttcgtt cgtcaaccct
    22501 gcttctctaa atggtgcagt taatggatcc agtccaacat agatgaatac tccgtctgct
    22561 gccaaatcaa actcattgcc ggttttgaca ttacgtgctt tcacacctgt cactaccatt
    22621 tcattcccaa cgatttcgtc aacgactgta tcccacataa aggaaatttt ttcattcgca
    22681 aatgcacggt cttggatgat cttttgtgca cgtaattcat ctctacggtg gacgatgact
    22741 acttcagaag caaattgtgt caaatagatg gcttcttcta ctgctgagtc tcctcctccg
    22801 atcactacta gacgtttgtt acggaaaaac gcaccatcac aaactgcaca gtaagaaacg
    22861 ccacgtccag caaattcttc ctcgccaggt acgcctaatt tacggtggat acagcctgtt
    22921 gcgataatga ctgtttttcc ttcgtaagtt ttatcttcac aaataacttc tttgtggctg
    22981 ccgtgatcct tgatatcttg cacgattcca taagcatttt ctgtcccaaa tcttgaaaca
    23041 ccttcataca tcttataagc tagatcagga cccatgattg aatcgaaacc aggatagttt
    23101 tcgatttctg ccgtattgtt catttggccg ccaggggcgc cttgttcgat catcaatacg
    23161 gataagtttg aacgtgaagc atataaagca gcagtcatcc cagcaggacc tgcgccaata
    23221 acgattacat catacatatg tagattctcc tttacttaat caagtaagca tactttacag
    23281 ccaatgcgaa aaaaagtcta ttgttctgct tgcgtttctc gaatagcttt attatactcg
    23341 aaaaatccgc tgaaaaacaa tagactttta taaatcattg atcaatcatt cgaaagattt
    23401 tctccatttg aatcaattac ttttttatac cagttgaatg atttcttctt cgaacgggct
    23461 aatgtgccat ttccctcgtt gtcacgatcg acataaatga atccgtacct tttcttcatc
    23521 tcacctgtac cagctgatac taaatcaata catccccaag tcgtatagcc taatagatcg
    23581 actccatcta attctactgc atctttcatt gcttggatat gtgctgccag atagtcgatt
    23641 cgataatcat cttccacata accattctca tcgggcgtat cgactgcacc taagccgttt
    23701 tctacaataa atagtggttt ttgataacga tcataaatat cattcatcgt tattctcaag
    23761 cctaatggat caatttgcca accccattca cttgcttcca gatatggatt tttgacagaa
    23821 gcaaagatat ttcctgcagt ctgttccaag agttcgttgt cagtagtcga aactcgagaa
    23881 gaataataag aaaaggagat aaaatcaaca gtatgttctt tcagcaatgc ggcatcccca
    23941 ggttccattt cgatctgaat gccatttcgt tccatttctt tcaatgcata agcgggatat
    24001 tctcctcgtg actggacatc aatgaagaaa taattttctc gatctgattt tctagaagcc
    24061 caaacatctt caggattgca agtgtatgga taatatgctc ctgctgccag catacagcct
    24121 actttgttct ccggatctac ttcatgagca atcttcgttg cgatggcact tgcaaccaac
    24181 tcatgatgtg ccgcttgata tttgatttgt tcttcatttt ctccttcttc aaaatacagc
    24241 cctgcgccca tgaatggtgc atgaaggatc atattgatct cattgaatgt caaccaatat
    24301 ttgacaagtc ctttatagcg attgaagatg actcggcaca gattttcata aaaaccaacg
    24361 agttctctgg aacgccacgc accatattct ttgatcaaat gcatcggaca atcaaaatgt
    24421 gtaatcgtca caagcggctc gatcccgtac ttttgacatt ctttaaagac atcctcataa
    24481 aatttcaacc cagattcatt tggctctttt tcatcgccca ttggaaaaat acgactccaa
    24541 gcaatggaca tccgataagt cttgaacccc atttctgcaa ataaagcaat atcttcctta
    24601 taatgatgat acatatcgat cgcaattttt gctggataaa aatgattttc atcaaattca
    24661 aacattttct tttttccagt aatgacagga aaacggtctt ctccgattgg cacaacgtct
    24721 acgttcgcta acccacgtcc gccttcatca taaccgccct cgcattgatt ggctgctgta
    24781 gctccgcccc ataaaaaatc ttttctgaat cccatcatac ttcctccatt acatgtgtat
    24841 tgcttgataa ttctcttgcc agttcatctt ggacttcgat tccttctgct tttaaccggc
    24901 tgattgtttc cttgaattgc ggtaaatact gctgatgtgc aataaacaac tcatccatta
    24961 tccgttttgc tgtctttcct gatcgtataa gcggattgat cgtaaacgct tgcaaagctg
    25021 ttccataatc accagtcacg gctgcttcga tcgtcagcaa ttccatattc ttcattactt
    25081 gcaaccagcc tctttcagca gcaggaaggg aaccaaacgc tacatttcgt gcgccttgtg
    25141 cgccaacata ggcagtgact tctactacac aatctgccgg cagatcagga acagctccat
    25201 cattctttgt agataccacg atctgtgttt ctttatttcc atagatcgaa gcaatcgttt
    25261 cacaggcagc atctgaataa taagcaccac ctcgtttttg caattgttca ggtttatgat
    25321 ccaattctgg atctttgtat aattcaaaca gctctgcttc tgtttctttc acttgctgcg
    25381 ctcttgtacc aatcgtttga tattcttcta aagcatgttc cagcatttct tcttcacggt
    25441 agtaatagcg gtggtagcca caaggaatca tgttcatttg ttttaattgt tctttgaaaa
    25501 acgggatatc aaagatattt ttcggcaatc cattgtcttc atcgtataaa cgatcaatca
    25561 attctgccgt gcggtcattt cctttattat ctgctacttt gtgccaatgg aagtgattta
    25621 gaccggcaaa cttgtagatc agctcttctt ttttcttgcc aagcattgtt ggttctgtca
    25681 tcatcgccat tactggcaca ttgcataatc cgatcacttt atcccatttg ccatatctga
    25741 taactgcttc tgtcaccatc ccgcttgggt tcgcgaaatt gatcagccaa gcatttggac
    25801 ataaacgctt catgtcttca acgattgcca aaaccactgg aacggtacga aaagctttga
    25861 atatcccgcc agcaccattt gtttcttgcc caatcatccc ataataacta gggatccgct
    25921 catctttgat tcgtgcatct aattgaccga cacgaaactg agtcgtaaca aaatccgcat
    25981 ctttcaatgc ttcttcccga tccagagttg catagatttt gacttcatac ggagaagcat
    26041 cccacatacg ctgagacatc tgactgacga tctcaagctt ttcttttcct tcttctacat
    26101 ctacgagcca gatttctcgt atcggtaatt cctcatatct tttgataaat ccttccatca
    26161 actcgggggt gtaactgctg cctccaccaa tcgtagcgat tttgattcct tttgccatgt
    26221 tctcactcct ttacttttta cacttttatt atcttttttg taaacaaaac aaacaataga
    26281 aattcgtttc tttccagtaa catttacaaa agttagtgtt ttcttctgga tttttgtaaa
    26341 tatcttgtca aaaaatgtct ttattaagct atataacata aatccgttta caaatcattt
    26401 tcaaaaaagt tataatttac gtaaaaaaac aagaagaagg tatctacatg ttattacgtg
    26461 aaaaaatcaa agaaatctcc ttttctcctt cagaaaatga agtgattcag tttctcttaa
    26521 gcgattctcc agcacttggc gaaatgacca tccaagagat tgctgagcga tgttatgtcc
    26581 acccgtccac actcatccgt gtagcaaaaa aaatgggttt tgatggctgg gtcagttttc
    26641 gtaatgcttt tttagctgaa acagcctatc ttgaagggaa ttttcaagac gtagatgcga
    26701 atcttccttt ttccaccaca gacggtatta tgacgatcgc aaataaaatg gcttctttag
    26761 aacagatgac gattcaagat acactttctc tcattcatca tgaccaactg caaaaagcca
    26821 aacaactaat gcttcaagca aaaaaaatcg tgattttcgc cagtaactcg aatactttga
    26881 tttctcaaga tttcatgctg aaaatgaagc gcatcaaaat ggatgtcacg attgtcacca
    26941 cttttggtga atcgttctat gaagcgtaca attgcacgag tgatatgtgc gggattctga
    27001 tttcttatac gggtgaaaac aatgtgatcc ttcgtaccgc agaaatattg aaacaaaatc
    27061 atgtgccaat tttagctttg accagtatcg gtgattcaac gctatcttca atgagtacag
    27121 ccgctttgcg acttacgaca cgcgaacgac tgtattcgaa aattggtaat tttacgatca
    27181 atacatcgat ttgttatctt ctagacgtgt tatatagttg tgtttttgca gaaaattatc
    27241 aacagaattt agtccatcta atttctgtag gacaaaaatt tgataaacgc cctatcagct
    27301 cagccatcat gaaagagcct ttcaattttg aaaataaaga agtggactaa aaagaataat
    27361 taagcctagt ccattctttc acctaaccgt tcaggtcagt gcggtgataa gtgcatcgtc
    27421 tggcccgacc gtctcccctt gattttccaa aatatccaaa taatccgtgt aattggtgac
    27481 gatcacaggt gtttctaaac tgtatccttc ctcttcgatt ttcttcttgt cgaagcggag
    27541 caacagatcg ccttgtttca ctctttctcc ttgctgcacc aagcttttaa aatattttcc
    27601 ttctaactga acagtatcca atccgacatg aatcagcaat tccagacctg cttcagaaac
    27661 cattccgatt gcatgtttcg tcgggaacag ggtcatgact gttccgtcaa aaggggcacg
    27721 tacttctcct ttttcagggt agatcaagat tcctcgacct aatgttcctt gtgcaaaagc
    27781 tgcatccttt gctgcattca gtggagcgat ttgtccttgt atcggtgttt ttacaatttc
    27841 tttacctgat gttttctgtc tatcatctgt ttcatctgct tcctccttcc aaaagaaaaa
    27901 agtcagcgca aaggatacca ccatcgcaat ggcgatccca atcaatacat taatcacatc
    27961 tgttaaatta ccatctggtc taatatagtt gggaattgta aagatgccta gtccaccttg
    28021 attccaagaa gttaaattca ttaacccact atacgcgccc cctaacgcac caccgacaca
    28081 ggtaaaaata aatatttttt tacgtggcaa tgtaaaccca taaatagctg gctctgtaat
    28141 ccccacaata ccagaaacaa tggctggtat tgcaattgct ttgtcttttg gatttctaag
    28201 cttaaagtag agtgcagtaa gtgctgcagt ttgtccaaaa gacactactg caacaggtgt
    28261 catgatattt tgccagccat tcacagaaaa ttgcatcaat cccattggta cgattgccca
    28321 atggagacca aacatcatta gaatttgcca gaagaagcct acgattgctc cataaataac
    28381 tggcgaaagt gctaaaagtg tttcaaatcc agccattaaa atatcagttg cgaatgtgaa
    28441 tgctggtcca acgagtaaaa aacctaaggg cattgtaata agaacggtga agaatggaac
    28501 gaagaaattc gcgatcatct caggtactaa ctttttaatt ggtttctgaa tttggctagc
    28561 taacaacaca ataaagataa tgggaatgac agaagaagcg tagttacgcg caatccaggg
    28621 aatgccgaat actgtttgat aaataggtgc ttcaaaaata gtgccagaaa atatggtcgt
    28681 cagtggtgct tctgctgcac cactcaaagc ttccagcgac aagctaggat tcattaatgc
    28741 tgccccaagc aacatcccta cgaattcatt tactttaaat ttacgggcag ctgttaaacc
    28801 gataataaca ggcataaatt ggaatacaga atcaccgata ttatcaaaca taacaaatgt
    28861 gccagaaccg cggtcaaatg cgcctaagaa gacaagtaat gctgtcagtc cacgaatcat
    28921 cccaccagca gctagaatag caaggaatgg ctggaaacag cccgatattc catcaataag
    28981 catatcaaac ggtctcattt tttctgttga aacagcctca tcaaaagtaa ggtttcctgc
    29041 tcttataacc tcttcaaaca cgactggcac atgattcccg atgacaactt ggtactgtcc
    29101 tcccgctcgc attaccgtca caacaccgtc catatttttc aagatctcat catttgcttt
    29161 ttcttcgttt tttaatttga aacgaagcct tgtgatacaa tgtgtcaaac cattgatatt
    29221 ttccttcccg ccgacatttt ctacaatttt gctcgctaat tcttgatatt ttcccatttc
    29281 tattccctcc aatttattag tggaaggttt agccaactga atgtcactac ccttatttca
    29341 tgcgttcttg ttaatttatc ctcctttttt atttatttac aacgattctc atgcactaaa
    29401 cgcgcagtat ggatcgttaa atacattttt tcgtccgtgg acatctggta gttgaatatc
    29461 tcggctaaaa agtctgctat tttctcaacg caaccaaacg tatccttata ttttttttga
    29521 ataacttggt acaattcctc agaaacttct cccacttctg atttttctcc atctaggacg
    29581 cgtgtcagaa aaaaacgtag gtgtgtgacg aatcgaaaaa aatggactga atcttcatca
    29641 aatgtaatct tatagtaata gcggacgata tttagaatat cctgtattgt tgtagtaaac
    29701 aggtaggtat tgtcttgttg gctttgaatg ccttgtgcat tgactaaatg caatgcgata
    29761 aatccggctt catcttctga taacttgatg tggtatttat ccgagatttt cttcactgtt
    29821 ttttccccga tggcatactc tttaggaaat agttgtttga tatcatacag caatacattt
    29881 ggtacaaata ttcccatttt gtgacgttcg atagccgtat acaaatgatc cgtcaatgca
    29941 atatacactg aatcattcaa ctggacttgt agagcctctt ttgtatattc aatgatttca
    30001 caagaaattt ctaagtactc aagaggaatc gcttgtgcca attcttggaa ctgttctgaa
    30061 agtccgtcat ttgtcaactg aaattttttg tcgatgcatg agtcaaaaat cgggtcaccc
    30121 actttctttt tgaatgccaa accttttccc atcacgatca tttcctgatt tctttcatct
    30181 ctggttatta cgacgttgtt attcagtact ttctcaatca gcatgtttta ctcctttcca
    30241 tgatatatag ggctgtatcc aaaacaaaaa gaaaaaccca aaaagccgtc catggaacca
    30301 tgggagactc atcaggttta gcttgcatca ggcaatcact atccaagttg tatcgttttc
    30361 tttatattat cgaaagcaag cgcttttgtc aacgctttct tagtggctat taggcacttc
    30421 tgactagctg tcgaatcttt tttaaatctg gtgactacga cactaccgct cacagatgaa
    30481 gaatctggga agctctgttt tattttcact ctagcgctta ggtcaaaatg ctactatgct
    30541 actgtcccaa cttctaatat acggtgttcc agtatcagct tatcggtgat aagccaaatt
    30601 tatgcaaaaa tctggaaagc cttttttaca atttttttct tatcacctaa agctgaacgg
    30661 agccctgtca caaccacttt ttacacacca ctgctttgca gtttgtacat atctgcatac
    30721 aaaccatttt gcgctagaag tgtttcatga ttcccgcgtt ctactatatg acctttgtct
    30781 aaaactaaaa tcaattctgc atcccgaatc gtggacagcc gatgggcaat cgcaatcgtt
    30841 gtccgtcctt gtcgcatttt tgtcaatcct tcttggatca atccttctgt ttcagtatcg
    30901 atatttgctg tcgcttcatc caatactagt attttaggat ctgtcacgat tgttcgcgca
    30961 aaagtgatca gctgtcgttg tccgctagaa tagctggcac cgtgttcgat cacttttgag
    31021 tgatatcctt ttggtaattc atcgatgaac ttgtttgctt gaacaaactc agctgcatgg
    31081 cgaatctgtt cgtctgtgat agttggattc atcatccgga tatttcctgc gatatctcca
    31141 taaaacaaga aggcatcttg caagaccaat cccattttct tacgtaactc ttccattgga
    31201 tattctcgaa tgtcatgatc atcaatcaag atctggcctt cataaaattc gtaaaaccgc
    31261 atcaatacgt tgatgatcga gctttttccg cttccggtgt gccccactaa tgcaacggtc
    31321 tctcccggat tagcgacaaa agagatattg ttcaatacat tgtgctctcc atcataagaa
    31381 aagcttacat tgcgaaactc gatcttacct gaagtaatcg tttgatctgc tccaggattt
    31441 tgttgcggtg catattcttc tgtatccatg attttcaaaa tacgactacc ggcaaccatt
    31501 ccatctgtaa aggtgctcaa aaagtccatc atttgagaca ttggattaaa aaatccctgg
    31561 acataggtcg taaatgcata gatcagtcca acttctacgg gagaatgcag cgcatcgatc
    31621 ccaaacaaag tcaacgccaa cgcaatcgcc aatgcatata gaaaattgat gatcggactt
    31681 aacagaatcg aattcgtttt gatcatcgca tagcgcgtgt ctaaatactc gttgttcact
    31741 tgttcaaatt ctttagctaa acgagcttct tggcgaaact gctggataat acgcatgccg
    31801 gaaatcgatt cattcaattt cgtgttcagc tgacttaatt tttcccgcat attccggtaa
    31861 atacgagaac tgaatttctg gtaataccag atgacgattc ctaaaatcgg caagaacacc
    31921 aaagtccaca aggcaatttc tccattgatt tgaaacatgg caatgaagga agaaacgact
    31981 gcaaagatcc cagtcaatac catcaaaaag acataccaaa attcaaatag cgtttctgtg
    32041 tcatttgtca ctcgagaaac ggtcgatcct gccggtgtct gatcaaaata acgcatacct
    32101 agtgtatgca gcttttcaaa taatcttacc cgaacatact gataggtctt caatgaagcc
    32161 atggaataca aataccattg gaaaaaccaa atgatacttt tgactatgac tccaaacaag
    32221 tacataccag caaaaaacaa aataacttgt gtcgtagccg actgtttagc cagatgatca
    32281 tccataaaag tttggatgat ccttggcaat aggacattga tcacagacag agcaaacgca
    32341 aataagatag caacgatgaa cgttcttcta aaaggtttgg cgaacttcat cagtcgtttg
    32401 atgatcttct tctgttcttt aagtggaatc gattttgtcc attccgattg ataattttca
    32461 tccatcaggc ctcgcctcct tctatcttcg cttctaattg ctgtttctcc cacattcgtt
    32521 cataccagcc gcctaatgca agcagctcat gatgggttcc tcgttcaatg attttccctt
    32581 gatcgacaac aatgatttct tttgcatgca tcacgctgct taaacggtga gctgcaatga
    32641 tggttgtttt ctcttttctt atttctttta aattatgcaa gatagcttct tctgttttgg
    32701 catctactgc agaaagtgca tcatctaaga tcagcaattc aggatttttg atcaatgctc
    32761 gagcaatcga aatcctttgt ttctgtccgc cagataaaga gactccacgt tctccaacta
    32821 aggtatcata accttttggc aattccttga tttcattgtt gataaatgct aaagaagccg
    32881 catgctctac ttcttcttct gaaagttttg gattagaaaa acggatatta tctcgaatgg
    32941 tcatcgaaaa caagaagtga tcttgaggca catagccgat tgatcctaat aaagcattca
    33001 atgaatattc tttgatcgaa cgtccgccaa attggatttt tccttgataa tcatcgtatt
    33061 cacgcatcag taatttgatg atcgtcgttt ttcccgcacc agttttccca acaataccta
    33121 atgtttctcc ttcttggatc acaaatttga tttgttctaa tgctggtgat tcttctttcg
    33181 gatagttaaa agaggaaata gctacattca gctcgccttt tgccggggtc tgcactgcac
    33241 ccagttgttc cacgatatgc gtcttttcat gcaaaagttc attgacgcga tcataagaag
    33301 catttcctct ttccaatacg ttgaacaatc gaccaatcgc aaacatcggc caaacaagca
    33361 taccgatata gctaataaat gagaccatct gcccgatcga gatcgtgcct tccaagacat
    33421 actttccacc taaaataatt gttgcaacat aagacagacc gataatcaag gtaatgaatg
    33481 gatcaaataa ggagtctaaa aaattgactc gtttgttttt aataattgca tcttcaatct
    33541 tttcttcaaa atccgcgaga tcttcttttt cttgtccaaa tgttttaatg actttgattc
    33601 cagtaatact ttcttgtgtc ttatcattga tactagaaaa agcttcttgc gaatcacgaa
    33661 aagcatcgtg gagttttgac cccagcactc gtgaagtaac ggctagtaaa ggcagaggaa
    33721 tcagcgcaat cagtgttaag cgccaatcaa taaacaaaat catcgcaatg atcgttgttc
    33781 cgcccgtgat cactgaatct gcaaaggtca agataccagc tcctgctaca ttttggattg
    33841 cattcaaatc attcgttgca tgagccatca aatctccagt ccgatatttt tggtaaaaga
    33901 gattgtccat ttctgtaaaa tgtttgaata gctgctgccg aagttctttc tccaaccgag
    33961 ctgcacttcc ccaaatattc gtccgccaga tataacgaaa aagatattgt aagagagctg
    34021 caaggatcaa taataagatc caccaggaaa tgatctttat tgataaattt ttatcggcaa
    34081 tctcatcaat gatgatccca atcacctttg gaggaactaa ctgtaccaag gccactgcga
    34141 atagggagaa aatcccaatt aaataatgtt tcttttcctg tcgaaaaaac caacctaatt
    34201 ttctaaaaat cgacatttta ctcctccttt gtctttatca agagcatagg attgtctgtg
    34261 cttgtctttt ttgctgaata gtctgaatag tctaacaaat tacgaaataa aaaacggact
    34321 gaaaaaatca gcccgcaaaa agtctttggg tcagaaccta ttttttccct tggttcttca
    34381 tttgctgcat catctggcga attttcttct cagatggttt ttgccccatt gacatcatca
    34441 ttgatcgcaa catgtcctca ttaacgggag gatttttttc taaatagtct ttcatatatt
    34501 tacgcgctag gaaaaagcca cctactgcgc cgattagcaa agcaataata gcaataagta
    34561 cgacaatacc tgttgatacc atataaatct ctcctttcta tttccgaatt gctctcaatg
    34621 atttatttta ctaaagttta ttgaaaaaag aaaggcgtat tctttagttt catgcaaaaa
    34681 atagtggttt ttttagagaa gaacaaaatt tctttcatta accagcaaga cacgccacga
    34741 aaaaaaatta ggcataccga atatttttct ttaatttttt ctttctgagg tgtagaatgt
    34801 aatcgtagat aaataggaaa ggtggttttt tcttgaataa tcaatccaac aaacaatttc
    34861 ataagcatcg acttattgaa catacaaatg gtttatcttt agctgaagta aatggaacag
    34921 tggatgtacc caagggaaaa agttttttta aaacacttct tgcttactct ggaccaggtg
    34981 cattagtagc agtgggttat atggatccag gtaactggtc cacttcgatc actggtggac
    35041 aaaatttcca atatttattg atgtcagtta ttttgatgtc tagtctgatt gctatgcttt
    35101 tacaatatat ggcagcaaaa ttaggaatcg tgacccaaat ggatttggcc caagcaatcc
    35161 gagcacggac aagtaagaca ttgggtgtca ttttatggat cttgactgaa ttagccatta
    35221 tggcaacaga catcgcagaa gtcattggtg cagcaatcgc cctctacctg ctttttgata
    35281 ttccattagt gattgccgta tttatcactg tgttagatgt tttcgtcctc ttattgctga
    35341 caaaagtcgg gtttcgtaaa atcgaagctt tggttgtctg cttgattttc gtcattttat
    35401 tcgttttcgt ctatcaagtt ggcttgtcta accctgattg gcacagtttg tttgccggct
    35461 ttatccctaa cagtcataca ttctcagact caccggtcat cggcggtgaa actccgctga
    35521 ctggagcttt agggattatt ggtgcaacag tcatgccgca taacttatat cttcattcag
    35581 cagtttctca aactcggaag atcgatcacc aagatgaaga agatgtagca caagcggtac
    35641 gcttttctac ttgggattct aatatccagt tgtcaatggc ttttctagtc aatgcactgc
    35701 tattgatcat gggtgtcgct gtattcaaaa ccggttctgt ccaagatcct tccttttttg
    35761 gtctgtatga agctttatct gatccatcag ctatgagcaa tggtatcttg attgctgtcg
    35821 caaaatctgg tatcttgtcc actttatttg ctgtagcact tcttgcttct ggccagaact
    35881 ctactattac tggtacgttg accggtcaag tcatcatgga aggcttcatc catatgcgta
    35941 tcccgatttg gctaagaaga ctagtcacac gattgctttc tgtgattcct gtattgattt
    36001 gtgttttgtt tacaagtaca aaaggaacga tcgaagaaca taccgctttg aataacttga
    36061 tgaatgaatc ccaagttttt ctggcttttg cattgccatt ctcgatgatt cctttactga
    36121 tgatgaccaa tagccaagcg gagatgggga gccgattcaa aaattcattt gttgtaaaac
    36181 tacttggctg gttctctgtg attagtctga cttacttgaa cttacgagga ttaccaggtc
    36241 aaatcgaaag tttcttcggc gagcaagcca cctcagctca actagcattg gctgatcaaa
    36301 tcgcttatgt aatcattgtt cttgtccttc tcctgttggc atggacgatt ggtgatttat
    36361 ataaaggaaa caaaaaatat gaacagcgtt tgcaagaaac gatagaagaa atcgaataga
    36421 aactgattct tttacacaaa ggaggttgtg acagaagtgg gcagcttcaa gaaataagaa
    36481 ggaatttaag aaaattgctt ttcaaatttt tgtgaaattt cggcttattt ttcgaagaag
    36541 ttacttctgg aacaccgtgt atcagaggtt gtgaaaaagc tgtcctgcat caagtaataa
    36601 gaacagccaa acaaaaatag ttcctcatat tttacgtttg gttgagctta ttaccgcaga
    36661 tgcaagcttt tgaacaccgt ttattcggaa aaaggaactg cgacaagact tttgtcacag
    36721 ttcccttttt atttagaaga taaagctttt ttcagattca atttactgaa aactagcatg
    36781 cttgttacta cgatgaattg tgttaatggc acgcctgcgc cagtgtagac acttaaaaaa
    36841 taatcctttt tttctggcgc ataagaacaa aaccacgaga caccgaaaag aaaaaccaat
    36901 tgtgtcagta ttaatccggt gactacaatc aaagaggaac tataataggc acgatcagct
    36961 tttgcaaata cggttcctag tatcataagt aatatccctg ctattgaagc aatcagcacg
    37021 ggaatggcca gaacttggta aatttttatc atgacattcg cgacttttgc tgtttttgtc
    37081 aactggaaat caggtgttct cggatcactg ctatcgatcc aattatccat tagtggaaga
    37141 ttcaataatt cttgggcctg ttcgacttgt tcttgcgtcc cgaaactata attccctcct
    37201 tgatggaaat ctttgtatcg ccatgcatag gcatatcctg atttcagaaa agcaaatacg
    37261 attggcagat cattcatcgt ctttccatcc cctgatccca tcagatagaa ttcttttttc
    37321 tttttgatag ttcccttttg ataggcttct tctaattctt gattgacttt tttccaaaaa
    37381 tcattggttt cttttgcatt gttttggtaa taaccagatt cgcttaccac atctcgcaat
    37441 gcccaaaaaa tgatatctcc tggtaattct tttacttttc cgccttgggt ccacgcatga
    37501 gtcgtataca attcgtcaat acgagtagaa acagtatgaa atgtaggaga tgcgtctaat
    37561 gctttattta atgcagcatg cgagacccat atctcactgt tttctttatt taagttcgtt
    37621 tcgtcatcta ttcggatcag ctgtttcatc aaacgtccaa attcaccact cgaacggtca
    37681 ttgatggtgg caataccata tgttgtctcg tttttatatt cgatccactg atagacgatg
    37741 gagaaaccga ctagagggag taaaaacaat gaaagtttca gccattcttg tttcgaaagg
    37801 agaaatggtc gttcttttaa cttctttgct tctttgaaag aaaagtcttg tcctagcaag
    37861 atctgaacga aactgatgcc taacgcgact acagtaaaag gaagaagcca gatggaatct
    37921 tcgcgaatga accaaaagaa aggaaaagaa acagctaacc ctaaaaacca cggcagcaat
    37981 tttctgcggg aactatattt acggtaataa atagctagta aacaagcgat aatgatctca
    38041 acagccggga tcacgagagc attacgatac gttcgagtgg aatactcatg tgtaaaacta
    38101 atcggagaaa acaaaaaata aaaatagata cttaaaagaa gatacttgtt cttcacgatc
    38161 ggtttaaacg ctagtgcaac caatatgctg gcaagaatac tgaacatgcc gaacaaaact
    38221 gcataagaaa gatgcagttt atgtgcaagt actaaaaaca agctgtagga gatccctttt
    38281 gaaagtgtcg tagtcgtgta ctcccctagc catttaccat ccaaaatatt ttgagcatag
    38341 tgaatcagca gctgatcatc ataggttgcc gacatatctg ttccatagat tgctttacta
    38401 tataaataca accgaataat tgtaaataat acggctatgc caagaataaa gtatgtatgt
    38461 ttctttttta gttggttgat caagggtttt aaagtgttca gcatcttttc gtcctttcaa
    38521 tcacgttttg ctacttgctt gtgaatcttt tcgtttttta cttttctatc agtataccct
    38581 gtttgtctga atttaaaaag agggggcgaa aggagcatgg cgaaaacgac tgcaaaaagc
    38641 actcctttgt ttcttaaaca aaaaaggaca aggcagactg cccaatcctt ttttgttgct
    38701 gtcagataga aaacttaaag tgtgacttct tctgctaata actcaggaaa cacttcggga
    38761 tcgaatgctt gcgctttgaa catggcaatt tcttttccat atggtgcgta agctttcttt
    38821 ttatctttac cgacatatgg agtttctaag attttcgaaa tgttgtcaaa cattggatca
    38881 tggacgattt tgttcaatgt gtcaaatcca attgttccaa aaccgatatt ggcatgccga
    38941 tctttatgag aaccttgtgg atttttagaa tcgttgatat ggagtacttt aagccgatct
    39001 aatccgatga ccttatcaaa ctcttccatc acacctgaga aatcttcttt tatattatat
    39061 ccggcatcat tcgtatgaca tgtatcaaaa gtaacggata atttttcatt caattttact
    39121 ccatcgatga tttgcgctag ttcctcaaat gttcgaccga tttccgtacc tttccctgcc
    39181 atcgtctcca aagcgatctg tgccgtctgt tcttttgtca aaacttcatt caaaccctca
    39241 ataattctag caatcccacc ttctgcccct gctcctacat gagcgcctgg gtgaagggtg
    39301 atttgagtag cacctaaagc ttccgcacga acaatctctt ctctcagaaa ttgtacagca
    39361 aatggaaaca tttctggttt gatcgtattt cccaaattaa tgatataggg cgcatgaacg
    39421 acgatatttg ttagtccgtt ttctgccata aatgcttttc cggcatcaat attcatctct
    39481 tcgatagctt ttcttctagt attttgagga gcgcctgtat agatcataaa ggtcgaagat
    39541 ccgtaactgg ctgcttcttc tgccgcgcct aacagcattt tctttccgct catgctgacg
    39601 tgtgagccta ataacatgga tggattcctc cttgaaaggc aattgtttcg aacaattcct
    39661 atatattggt aagatgatgt acaaagaaaa tggtttgttt ttctttgccc tgttctccat
    39721 agtaactgaa actttctcta aagaaaagct taagaactct ttcataaaaa agatcatttc
    39781 ctcacgtttt atctcattcg aaaaagaaat tcctgtcaaa aacagccttg tctgtttttt
    39841 tgtcttatta tttgctataa ttagcgaaat aaaaccatta gttgaggtga cccttttgcc
    39901 acgcaaaaat actcgaaaaa aacagaatag aagaaaaaaa gaaaaatggt ttgttcctaa
    39961 ggttattttt agagtgtttc aatcattgac tgtttttatt actgttcttc ttttgttgtt
    40021 cgcagctcta ggtatcggaa taggtgctgg ctactttgcc tatcttgtcg aagacacaca
    40081 attgccgact aaaaaagaac tgcagacaga actaggaaac attacggaaa cgtcaaagat
    40141 tgtttatgca gataatacag agatatcaaa aatccagaca gatctgatgc ggacgactat
    40201 ttcatctgat aagatttcgc ctttattgaa aaccgcgatt atcagtacag aagatgaata
    40261 cttcgataaa caccaaggtt atgtccctaa agctgtttta cgagcattag tttcagaagc
    40321 tactggcatc ggctcgtctg gaggatccac tttgacacag caattagtca aacagcagat
    40381 cctaaccgat gaaacaacat tcaaacgtaa agcaaacgag atcgttcttt ctgcacaagt
    40441 agaaaaatac ttttcaaaag atgagatcat tgctacgtat ttgaatgtct cgccatttgg
    40501 tcggaataat aaggggcaaa atattgctgg agtccaggaa gctgcaagag gaatctttgg
    40561 tgtagatgca aaagatgtga ctctgccaca agctgcttat atcgctggtt tgcctcaaag
    40621 ccctatcacg tatagtccct acacgaatac aggcgcgcta aaagaagatc tttctgccgg
    40681 tcttgccaga aaagattttg ttttattcag tatgtaccgt gaaggacaaa ttacgaaaga
    40741 acaatacgaa gaagcgaaag cctatgatct tacaaaagac tttctcccac aacaaatcgc
    40801 ggaacagact gacagagaat tcttgtatta taccgtgatg aacgaagcaa caaggattat
    40861 tgcacaacaa ttggcagaaa aagataatgc tgatatgtca gattcatcgg tcagtgaagc
    40921 ttattatcaa aaagcacagc aaacgatcca aaacaaagga tatacgatcc attcgacgat
    40981 cgataaagat atctatgctg cattgcaaac cggcgtagaa aattacggct atatgttaga
    41041 tgatggtgct ggtagccaag tggagactgg aaatgtattg atggacaata ggactggacg
    41101 tatctatgga ttcgtgggtg gccggaatta cagccagaat caaaataacc acgcctttga
    41161 tacagaacgt caagctggtt catccatcaa accggttctt gtttacggtc ctgcaatcga
    41221 tatgggactt gtcggatcag aatcccgtgt atcagattat gcaacgactt ggcaagaagg
    41281 agaaaatgct ggcgagaaaa tcgtcaatgc gaccaatgaa gggtcgaaca ccttccaaac
    41341 cgtccgtgag tcactggaat ggtccaacaa tatcccagct tatcacttat accaagacgt
    41401 attgaattcg ggtggatcga aacaatatgc ttacgagcac tacttggcga agatgaacta
    41461 cccagcaaat gataattggg gcgttgaatc tgctccttta gggacagtag atgtaacgac
    41521 gcttcaacag acaaatggat tccaggcctt agctaatggt ggcgtctacg aagaaggcta
    41581 tatcattgat tccatcacag ataatgcagg taatgtgatt tataaacatg aaagtaattc
    41641 tgtccgtatt tattccgaag caacggcctc gatcatgaat gacatgatgc gcagtgtgat
    41701 caatgaaaaa atcactacac cattcaaaga tgtcatttca agcttaaacg gaaatctagg
    41761 aaaagcagat tgggtcggaa aaactggttc taccaacgaa tacagagatt catggctgat
    41821 cgtctctact ccgtctatca ctatcagtag ctgggcagga cacgatgaca atactggaat
    41881 ggacagccaa gcaaggatac gttcggctaa ttatcttgcc aatctaatca atcaagctta
    41941 ccaagcaaac cctgagattt tcggcactga tgagaaattt gacttatcat ctgatgtgat
    42001 gaagaaaaaa gtatctgggt tcacaggaca aacagctgga aaagttactg tcaataaaaa
    42061 atctatccaa acgccaggaa aaacagtgga atcactttgg gcaaaaaatg gtccgaagaa
    42121 aagcgccttt aaattcggcg ttggcggtac tgatgaaaac tatacagatt actggaacac
    42181 cgcttcagct tatgctagag cgaatccaga gaccaaagaa tcagacgata aagatgattg
    42241 atctaataga ttaatcagaa ttaaagggag ctgcgacaaa aatcttgtca cagctccctt
    42301 tttccgaata aatggtgttc aaaagcttgc atctacgata ttaagacaaa tttatgtaaa
    42361 aatgtgaaaa gccattttca caaaattttt ctatataact ttactaacga aactgtttta
    42421 atttcttttc ttgtctttgg aataattcgg ttagtttttc tgcaaattgc ccttttactg
    42481 attctttcaa gtgtacatgt cggttagaaa attcttgagc agcttcttct gcagtcattt
    42541 ggtggttttg atctgtcaat cgtttttctg cccaaatttg ctgagatatc cccataccaa
    42601 ctgaacgtac atttgatcga tgtagatttt tctctaattc ttttagtaat tgcattaaaa
    42661 attcattcat aagatgattt ctccatagtt atttgtctga ttttttgacg ataggaacgt
    42721 tctagttcgt cttgctgctg atagagtttt cgtcgttcta cttgtagggc ttgttgataa
    42781 tgctcctgat aattcgaata acttcgtctt gcttgaaaca gaggctcatt atctaaagct
    42841 aagttcttta atacatgatg cagtcgctgc tcctcttgct ccatcaatcg taatccttga
    42901 ttcttaaagt ggatcaattc atcttcttga gcttctaatt gtcttcgttc acttcgataa
    42961 tctctttcca attcttctag tttcatctac ttctccttta attgaattgc tgtgctaatt
    43021 ctcgatctcg tgtcacgatt tcattgattt tgtcttggat ctccttcacc aatgtatcaa
    43081 aggcctcccc catacgtctt gccttattga ttcgcagcag atagacttct ttcgcagaat
    43141 cgactaagtt ctcttttgtg gcgacaacac tagctaatgc ttctatgatt tcctgttcag
    43201 tcaacgaagg ggcgacaaca cgtgcttgcc gcaagccttc ttgccatgcc attttttctc
    43261 tagctcaaaa attcctcttt gattgatgcg gataacttct tccatagcgc ttttcatttt
    43321 tgctgataac ttttccacta ctaacctagc ttgtgtgtca tctaagtaaa tttgttcatt
    43381 aactgtcagc cggccgccac ttaatcgtag ttttttacgt atgttgttca gttcttgcaa
    43441 ctgataggaa aacaactggg tcgcttggtt ttctttctgt tgttttcgag ctttaacatt
    43501 attttcattt tcaggaataa tcagctttcc attttcatca aacttccaag aggttaaact
    43561 gtgggaagca agcagattca agaatgctgt ttccatactg atccgaactt cagcacctgt
    43621 gccattacca ccaaaattac cgataaaatc ggcgtgattt cggtaattgg tgagcatacc
    43681 gggattttct tccacccatt tttttgcttc tggtgataag agatcatacg ggtccggccc
    43741 gtttaagccg acatttttcc attgtttctc tgctgctaca tataatgcta agaatccacc
    43801 taatgaatgg ccggtagtag aaatagcagc ttctccatat tcctttttta cttcattagc
    43861 aaatgcaatc gctgaattga tttgccccgc tgttttcaat ccactaacgg ataaaatctt
    43921 gtttgttgtt gctttttcag aaaaacttgc tcctggtcta ttcccataga gattcatgat
    43981 actccttgca tccgtttcca aatctttcca atcacttaag ttcgttcctg caaaagcaat
    44041 cactacttga gaaagatcca ctttaccgtt cttgattggc gctacaccca tcgcctgcat
    44101 gccgtccaat gagtctttga tttttaggat tttaaaatta taactatcaa tttttttaat
    44161 agaattttct ttataagtat gatcatagtc tctgtgttta ggatctagcc agtaaacagc
    44221 ctcactaata acactgtatt gtttgtctgc catcattaaa tatattcctc cttattatta
    44281 gaaaaaatta ctctgatttt atttactgta atcccctctt tttgtatact tttattttct
    44341 acgccataat ctcctaattc atttcgttct ttccaaaatc caaacgtaaa ataaacagac
    44401 tcatcgtgat tatttatcat tgtcacaaac atacgatatg ctcccgtcat ttcatttcga
    44461 gcagattttt caatcttcac ttccttgata tccgcaaaag tatttttcaa aactttgact
    44521 gactgttttt ctactgcgag taaatctttc tgtctttgtg cttctctttt gtccatatag
    44581 actttccctc cgattcctaa tatgacgatt aataaaatgg ctaagctgag ttttcttatt
    44641 ttccgagaca agaacgtgcc tcctttcttt ttatgtaagt attgatacct tcattattta
    44701 agtacgcgaa agtttgtagg aattttttta tttttgaaat ttttctccaa tttgctatgg
    44761 acgattggcg ctgcagccat cgcctgcatg ccatccaatg agtctttgat ttttaggatt
    44821 tggaattttg tttctccaaa gtttttaatg gcgccttctt tgtaactttt actatattga
    44881 ggatgttttg gatctagcca ataaacatgt tcacttatat atctatactc ttcgtctttt
    44941 actgtcacac ttcctccgcc tctccattag aataaataac tcttatttta ggagtagtaa
    45001 ccccttcgtt ttgtatttct tcattttcta caccataact gctaatttca ttcatttgtt
    45061 ttgaaaaaaa ataatcaaaa taaacagatt tcccttcatt atttgtcatt gtcacaagtg
    45121 caccataaca tccagtaaca gggttttttt taaattcttc taccttcact tccctgatat
    45181 ctgcaaaggt atttttcaga actttgacgg attgtttttc tactgcgagt aaatctttct
    45241 gtctttgtgc ttctcttttg tccatgtaaa attttcctcc gattcctata gcaagaatgg
    45301 tcaaaatgat gactatggtc tttcgtcttt tcttaggcaa atagaagtct cctttcgggt
    45361 tatgtatgat ggattgtttc tcctttaaga taagtacgca atcgagcctg gatttttttg
    45421 gattttttag agttactttt ctacttttct actttctttt ttatcacgtt ttcattttaa
    45481 agtaattttg atagtagtga acaaaatgta gcatctacgc acagattgtg tcgtatacga
    45541 tggacgtaag gtttgtttcc gccattttct ctttttttaa agtcacaaaa aaaaggggtt
    45601 atgacaaaat tttgtcataa ccccttttct cgaataaacg ttgttccaga agttggccac
    45661 ttttggaaca acctctaaat gattgatctt ttattattta gttgatcctt tttcttcgat
    45721 tccataaggc aagataatcg ttttttcttc gacttcttct ttattcatca ttttcgtcaa
    45781 taggcgcatg gataccgctc ctaaatcgta taatggttgt gtgatacttg tcaaacgagg
    45841 acgagctact tctgtcagta aagaattgtt gcttgtgatg atttcgaatt cttctggtac
    45901 ttttacgcca cggtctaata aaccatccaa gataccaatg gctaattcat cgtctgaaac
    45961 atagacagct gttgcaccgc tattatggat ccggtcaact agagacagac catctttaaa
    46021 gttgtattgt gcctcaaaga ttaatccttc gctatatggt agattgtttt cgcttaatgc
    46081 ttctttatag ccttttagac ggttttgacc attgatagga tcaatcagcg caccgctgac
    46141 aaaagcaatt tttttattgc cgctttttgc taatttattt actgcatctt ttgttgctga
    46201 tacataatca atgtttacgc tacctacttg ttcatctgga tcaatggaac cagctagtac
    46261 aacaggtgtt tttgaacgtg aaaattctcc acggatttcg tctgtgatat ggtgacccat
    46321 aaagatcacg ccgtccactt gcttagccaa taaattattc aatacattga cttctttttg
    46381 atcgttgcca tctgagtttg ctaagatgat attgtatttg tacattgttg ctacatcgtc
    46441 gataccacgt gctaaagagg caaagaacat attgctgaca tcaggaatga ttaccccaac
    46501 agttgttgtt ttcttacttg ccaagccacg tgcgactgca tttggacgat aatccaagcg
    46561 atcgataact tccaagactt tttttcttgt tgctggtttg acgtttggat tgccatttac
    46621 gacacgtgaa acagtagcca tcgaaacatt tgcctctctc gctacatcat agatagtgat
    46681 tgtttgcttt tccatttgtg ttctcctatt catattttat atgttttctg aaaatacgtc
    46741 ttacatttct aaaataggat atcaaataat ctcaacaatt gcaaccgttt tatctctgtt
    46801 ttcatgaaaa tttttacttt tttcagtttt aagaacgttg gtcttttaat caatcagtgg
    46861 tacaattatt gaatacaaaa ttaatttaga aagtaggttg ccaaatgaat aacgtaaaaa
    46921 tcaatgaact acgtcaatgg atggcacaac accaagtaga attggcttat atcagcgacc
    46981 ctggccacat cgcttacttt tccggatatg aaagtgatcc tcacgagcgt gtactagctc
    47041 tttttatccc tcttgaacaa gatcctttcc ttttcactcc tgctctggaa gttgaagatg
    47101 ctcaaaacag cagctgggcg tacgatgtac gtggctactt agacagcgaa aacccttggg
    47161 agatcatcgg aaaagagctt cgcgcacgct atgggcaacc tggtaatgta gggattgaaa
    47221 agaatgctct gactgtcgat cgcttcgatg ctatctctga tatcttgaca ggtgcagatt
    47281 cttacactga tctaacttct attatccagc gtctgcaact gatcaagacg gtagaagaaa
    47341 aagacaaact gatcgaagct ggtaaatggg ccgatgttgc ctttgaaatt ggcttcaaaa
    47401 cagtaaaaga aggcgtgact gaacaagcaa ttatcgcaga aatcgaatac caattaaaaa
    47461 aacaaggtgt ttcacaaatg tctttcgaca ccttagtcct tgcaggagca aatgctgcaa
    47521 gccctcatgg tacaccagga agcacgaagg tttcaccaaa tgaactagtg ttattcgatc
    47581 ttggcgttgt ttggaatggc tattgcagtg atgctactcg tacagttgct tatcaaaaac
    47641 caacagaatt ccaagaaaaa atctacaaca tcactcttga agcacagctt gccgcacaag
    47701 aggcagttcg cccaggtgtg acagcaggtg aacttgatca aatcgcacga aacgtaatca
    47761 acagctatgg ctacggcgaa tatttcaatc accgtttagg tcacggaatt gggacaaccg
    47821 tccacgaatt cccttctctt gtagaaggaa acgacttagt gatcgaagaa gggatgtgct
    47881 tctctcttga accaggaatc tatattcctg aaaaagtagg ggtacgtatc gaagattgtg
    47941 tgtatgtgac aagtgatggt tgtgtgccat tcacgactac accaaaagaa ttacttgttt
    48001 tagagtaatg aaaataaaaa aggtgggagc tggtccgctc tcaccttttt attctagaaa
    48061 tgagtattct tagtgattgg tagtcgaatc tgtcattgta tctttcattt cttttttagc
    48121 ttcttttgca tcttcttttg ccattttcaa catttctttt gtgtcatctg caactggttt
    48181 tgcttctgac attccttcat tgaccgtctc tcctgcttta tctgcagcat cttttacatc
    48241 gatcatgata tcttctgaag tatctttagc aatactacct aattcgtctg ctggtttttt
    48301 cagatcttct gctgtttttt tgaaacgatt agataaatca gctgattgtt ctttgaggtt
    48361 taacaagata tcatctgatt cacttcctgc ttcctctgct gattcactta ccttttgctt
    48421 aacattacct gctagatccc ctgcttgttg agaaagatct tgcgcttttt cttttgcaat
    48481 gcctgttaac tcagctgatt tttcttttgc catatctgtg taatctgaag ccatgtccat
    48541 caaatcatct gcttgagcag ccaattcatc ccgaagttct ttaccagatt tgggtgctaa
    48601 caataatgcg gcgattgctg cggctgtacc tccaactact gcacctaata aaaatccacc
    48661 ttttttagcc attcttattt tcctcctgtc gtttcagtat gttttttgtt tttaaaaaat
    48721 ttcatcgctg ttccaccgac ttttccggca acagtcgctt tagctgtcgt ttttccgatt
    48781 ccgccgactt tcgtcacgat gtttttacta gaatgattaa gttccgatac actttcactt
    48841 aaatcagcaa ctgctgtgaa caatggatcg atcgttgcga cttttccatt gatatccaag
    48901 agcagctcat tacttttgac cagcaagcct tctacttgtc tagataaaag atctacgtct
    48961 tttgtgacga tctcaattgt cgtatttgct tctgcgacag tctggtctac tttttctaaa
    49021 gttttggaaa cttgcaacaa tacgcgaatg atgaaaacga caagtatcgc aaaggcgatt
    49081 gctgcgatca atcctgcaat ttctcctcct gacatgatca aactcccttc ttgtctctct
    49141 tttctataag gatagcattt tcgtgttttt taaacaaata aaaacgagct tcaactactt
    49201 tttattcaat tagcagctga agctcgttag aaaaaaatga ttattttttc ttttcttctt
    49261 tgatttctgt tattcctttt ggattatgat cgatcttgct tccttctgct tctaatgttt
    49321 tacctaaaga catgatgtat tcacgcaaag gtgcccctac ttctggatgt gtcagaccat
    49381 attcaatact tgttttcata aaaccaaatt tgtcaccaac gtcataacgt ttacctgtga
    49441 atttacgggc aaatacgcgt tgtgttttat tcaacgtatc gattgcgtca gttagttgga
    49501 tctcatttcc agcacctggt tgttgttctg ataatacatc aaagatttct ggagtcaata
    49561 agtaacgccc aataatagct aaatcacttg gtgctttgct tggatctggt ttttcaacaa
    49621 agtttttgac attgtataac ccatcttcca gtacttcccc tgggttgata attccgtatt
    49681 ttgaagtttc atcgtgagga acttgcataa cggcaatcgt agaagcgtgt gtacgttcat
    49741 agtcatcgat caattgctgt gtcaatggta cttcgtcttc catgatatca tcgcctagca
    49801 taacgacaaa cggttcattt ccaataaagg ctcttgcttg aagaacggcg tgtcctaacc
    49861 cctttggatg agattgacgg atgaaatgca agttgacatc tgtcgtttct tctactaatt
    49921 tcaataattc gatttttcct ttttcactta ggtttgtttc taattcgata tttgaatcaa
    49981 agtgatcttc gattggtcgt ttagcttttc ctgtaacgat caagatgtct tcgattcctg
    50041 atttcaacgc ttcttcaacg atgaattgga tcgttggttt gtccacgatt ggcaacattt
    50101 cctttgccat cgcctttgtt gctggtaaaa atcttgttcc taatcctgct gctggaataa
    50161 ctgctttttt tactttcata tacattcgct ccttcaaaaa attgttaatg agaattcatt
    50221 ttcttttttc ccatcacgta acatgatttc ttttgcggct gttttgattt ctttttgttc
    50281 atacagcact tcatagattg tttgagtgat gggcatttcg atttccatca tttgggctag
    50341 ttccatcgct gcttgagtag tattcacacc ttcaacgacc attcccatat tggctagcac
    50401 ctcttctaga ggctgtcctt gtcctaacaa gttgcctgcg cgccagtttc tggaatgaac
    50461 acttgtgcaa gtaacgacta gatcgcctac tccgcttaag ccaataaagg tcagcgggtt
    50521 ggcacccatt gctacaccta aacggctgat ctcagctaat ccgcgcgtca tgattgccgc
    50581 ttttgcatta tcgccaaacc ctaagccgtg aatagcacca gctccaatgg caataatatt
    50641 tttcaaagct gcccccatct ctacaccaac cacatcaggg ttagtataga ttcgaaaata
    50701 atggttcatg aacagctctt gtacatattt tgctgctttt gcatctgtgc ttgccgctgt
    50761 gatcgtcgta atgtcatgct ttgctacttc ttccgcatgg cttggcccag aaagaacagc
    50821 aacagctttt cttttttctg tcgggatttc ctctaacaag atttcagaga ttcttttatg
    50881 cgttccctgt tctaatccct tacttgcgtg gatgatcact ggcttcgtat tcaatttggc
    50941 aactagttct tttgcaacag aacgtatcgc tttcgtcggg ataacgaata gtaccgcatc
    51001 tgcatctttt actgcatctt ccatatcggt gaatgctcgg atggaatcgg gtagatgatg
    51061 atctggtaaa taacgttgat tggtgtgttg ggtgttgatt tcttctactt gtgaagcacg
    51121 atgtccccaa atccgaacgt catgtccgtt ttcagcgagg acttgtgcta aagcagtccc
    51181 ccaagagcct ggtcctaaaa ccgctactct ataattcatt ttaccactcc tgaaattttt
    51241 ttcattttta acgctctaaa aatcaaataa tttgttgatt ttttcctaat gagcggtcat
    51301 aatccggtaa atttcctttt tttctacgcc aaataaacag aatcaatgtg ccgacaagta
    51361 aaacagcaga caataattga gaaatgcgga tcacacctaa aaagtaaagg ctgtctgtgc
    51421 gtaatccttc gataaagaaa cgtccgaaac tataccacat tagataacat aaaaggactt
    51481 ctcctttttt caaaagatgc ggtttcttcc gcaaaaccaa aagcagaata aatccaatca
    51541 aactccaaag tgattcataa agaaaggttg gttgtcgata gatcccttcg atatacatgt
    51601 tatcaatgat gaattgcggt aaatgcagac tttctaagaa acttcgtgtc gtgtctggac
    51661 catacgcttc gtggttcatg aaatttcccc atcgtccgat tgcctgcgcc agtaatacac
    51721 ttggtgcagc gatatctaaa aaggtccaca aagaaataaa atgatatttg gtaaaaaagt
    51781 acagtgtgat tgcacctgca atcaatcccc cgtaaatcgc taatcctcct gaacggatat
    51841 tgaatatttg gattggatca gctatatatt ggggaagatc gaataaaaca taatacaaac
    51901 gggcaccaat aatggaaatc ggcaatcccc acaacataaa atctgtcacg tcatctgctt
    51961 ttaatcctac ccgaacagct tctctgctgc tgagccacat ggcaataatg attcctgata
    52021 cgataatcaa tgcataccag tagataggaa ttcctacaag ctcaaaagca actcgattga
    52081 tttgtgctaa catattgtca ctccttgcta gtcattgcct gagttttctt caatcaagcg
    52141 acttaatctt tcttcaaatg ttttcgttgc gtcaaagccc attgtacgcg cgcggaaatt
    52201 catcgcggct acttcaatga tgattgcaac gtttcgtcct gttttgacag ggatttttat
    52261 ttgtggaatg cccacttccg cgatttcaac ggtcgtgtct tcagaaccca aacggtcgta
    52321 tttcttatct ttcgcccatg cttctagata aacagctaac tggacttgca tagagcctcg
    52381 taccgcactc gcaccgaaaa gattcattac atctatgatc ccgatccctc gaatctcaat
    52441 cagatgttcc aggattttag gaggttctcc gattaccgtc agttcatctt gctgatagac
    52501 atctactcga tcgtctgcga tcaatcgatg tccgcgctta atcagttcca acgccgtttc
    52561 acttttccct atcccactgt ccccttgaat tagtacacct aatccgtaga catcgactaa
    52621 cacaccgtgg acacttgttc gaggggctaa gcgcccatct aaataactag ataattcacc
    52681 tagcagccga gacgttgaga taggcgaacg cagcacagct atcccatttt cattggctgc
    52741 ttggatcatt tcttccggag ccggcaatcc tcttgaaata ataaatgcgg gcatatcttc
    52801 actacataat cttctcatga tgatcaaacg ctcttctggg atcatccgtt ccgcaaaact
    52861 gatttcttta ctgccaaata gctgtaaacg attatgagaa taataattaa aataaccggt
    52921 caactctagt cctgggcgtg agatgtcccc agtcacgatg acgcggtcta aacttttctc
    52981 gtcgcctgtg acgatttcca aagataattt ttttactaat tggctaactt ttacaacttc
    53041 tgacattcat gctacctcat ttctattaga gtgttctcta ccattttatc attgtctagc
    53101 caaaagtact accaattaaa aaaaacgttt ttatttaatt accaatgtaa tagaaaacgc
    53161 attcttaatt atatatgatt tgtcaaatta agctagaata aaatgcttct tgtacatagc
    53221 tcaaaaatat ttcgattggt gttctgtaat tcaatgattt tcttggaata taattacgtt
    53281 gattgctgac actggaaata aatgtctgat tcacttctct aaaatccatt gatttcggca
    53341 gtccattacg acgcagaatc ccgttagaat t
//
LOCUS       DACEQH010000012        52640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.012, whole genome shotgun sequence.
ACCESSION   DACEQH010000012 DACEQH010000000
VERSION     DACEQH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.012"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            117..971
                     /locus_tag="IW833_000721"
     CDS             117..971
                     /locus_tag="IW833_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ1306858.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            972..2309
                     /locus_tag="IW833_000722"
     CDS             972..2309
                     /locus_tag="IW833_000722"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ1306859.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            2314..2538
                     /locus_tag="IW833_000723"
     CDS             2314..2538
                     /locus_tag="IW833_000723"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ1306860.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            2540..3430
                     /locus_tag="IW833_000724"
     CDS             2540..3430
                     /locus_tag="IW833_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1306861.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            3439..4257
                     /locus_tag="IW833_000725"
     CDS             3439..4257
                     /locus_tag="IW833_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ1306862.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            4364..4813
                     /locus_tag="IW833_000726"
     CDS             4364..4813
                     /locus_tag="IW833_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1306863.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            4831..6513
                     /gene="recN"
                     /locus_tag="IW833_000727"
     CDS             4831..6513
                     /gene="recN"
                     /locus_tag="IW833_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ1306864.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(6568..6684)
                     /locus_tag="IW833_000728"
     CDS             complement(6568..6684)
                     /locus_tag="IW833_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ1306865.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(6757..7713)
                     /locus_tag="IW833_000729"
     CDS             complement(6757..7713)
                     /locus_tag="IW833_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1306866.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            7884..8252
                     /locus_tag="IW833_000730"
     CDS             7884..8252
                     /locus_tag="IW833_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ1306867.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            8422..8853
                     /gene="mraZ"
                     /locus_tag="IW833_000731"
     CDS             8422..8853
                     /gene="mraZ"
                     /locus_tag="IW833_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ1306868.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            8874..9833
                     /gene="rsmH"
                     /locus_tag="IW833_000732"
     CDS             8874..9833
                     /gene="rsmH"
                     /locus_tag="IW833_000732"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ1306869.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIGLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            9838..10239
                     /gene="ftsL"
                     /locus_tag="IW833_000733"
     CDS             9838..10239
                     /gene="ftsL"
                     /locus_tag="IW833_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ1306870.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            10240..12432
                     /locus_tag="IW833_000734"
     CDS             10240..12432
                     /locus_tag="IW833_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1306871.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            12458..13420
                     /locus_tag="IW833_000735"
     CDS             12458..13420
                     /locus_tag="IW833_000735"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ1306872.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            13434..14795
                     /locus_tag="IW833_000736"
     CDS             13434..14795
                     /locus_tag="IW833_000736"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ1306873.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            14821..15909
                     /gene="murG"
                     /locus_tag="IW833_000737"
     CDS             14821..15909
                     /gene="murG"
                     /locus_tag="IW833_000737"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1306874.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            15924..17144
                     /locus_tag="IW833_000738"
     CDS             15924..17144
                     /locus_tag="IW833_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ1306875.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            17300..18625
                     /gene="ftsA"
                     /locus_tag="IW833_000739"
     CDS             17300..18625
                     /gene="ftsA"
                     /locus_tag="IW833_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ1306876.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            18648..19889
                     /gene="ftsZ"
                     /locus_tag="IW833_000740"
     CDS             18648..19889
                     /gene="ftsZ"
                     /locus_tag="IW833_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ1306877.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            19906..20583
                     /locus_tag="IW833_000741"
     CDS             19906..20583
                     /locus_tag="IW833_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ1306878.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            20597..21196
                     /locus_tag="IW833_000742"
     CDS             20597..21196
                     /locus_tag="IW833_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ1306879.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            21211..21498
                     /locus_tag="IW833_000743"
     CDS             21211..21498
                     /locus_tag="IW833_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ1306880.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            21547..22329
                     /locus_tag="IW833_000744"
     CDS             21547..22329
                     /locus_tag="IW833_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ1306881.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            22350..23057
                     /locus_tag="IW833_000745"
     CDS             22350..23057
                     /locus_tag="IW833_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ1306882.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            23319..26117
                     /gene="ileS"
                     /locus_tag="IW833_000746"
     CDS             23319..26117
                     /gene="ileS"
                     /locus_tag="IW833_000746"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ1306883.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(26166..27692)
                     /locus_tag="IW833_000747"
     CDS             complement(26166..27692)
                     /locus_tag="IW833_000747"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ1306884.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(27707..28354)
                     /locus_tag="IW833_000748"
     CDS             complement(27707..28354)
                     /locus_tag="IW833_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ1306885.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(28538..28867)
                     /locus_tag="IW833_000749"
     CDS             complement(28538..28867)
                     /locus_tag="IW833_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ1306886.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            29044..29772
                     /gene="sfsA"
                     /locus_tag="IW833_000750"
     CDS             29044..29772
                     /gene="sfsA"
                     /locus_tag="IW833_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ1306887.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            29788..30801
                     /locus_tag="IW833_000751"
     CDS             29788..30801
                     /locus_tag="IW833_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1306888.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            30801..32078
                     /locus_tag="IW833_000752"
     CDS             30801..32078
                     /locus_tag="IW833_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1306889.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            32141..34843
                     /locus_tag="IW833_000753"
     CDS             32141..34843
                     /locus_tag="IW833_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1306890.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            34995..35312
                     /locus_tag="IW833_000754"
     CDS             34995..35312
                     /locus_tag="IW833_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1306891.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            35342..35662
                     /locus_tag="IW833_000755"
     CDS             35342..35662
                     /locus_tag="IW833_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1306892.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            35748..37208
                     /locus_tag="IW833_000756"
     CDS             35748..37208
                     /locus_tag="IW833_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1306893.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            37276..37497
                     /locus_tag="IW833_000757"
     CDS             37276..37497
                     /locus_tag="IW833_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306894.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            37528..37710
                     /locus_tag="IW833_000758"
     CDS             37528..37710
                     /locus_tag="IW833_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ1306895.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            37710..38123
                     /locus_tag="IW833_000759"
     CDS             37710..38123
                     /locus_tag="IW833_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ1306896.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            38246..39427
                     /locus_tag="IW833_000760"
     CDS             38246..39427
                     /locus_tag="IW833_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1306897.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            39816..39889
                     /locus_tag="IW833_000761"
     tRNA            39816..39889
                     /locus_tag="IW833_000761"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39850..39852,aa:Arg,seq:cct)
     gene            complement(39958..41097)
                     /locus_tag="IW833_000762"
     CDS             complement(39958..41097)
                     /locus_tag="IW833_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1306898.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(41396..42031)
                     /locus_tag="IW833_000763"
     CDS             complement(41396..42031)
                     /locus_tag="IW833_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306899.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(42144..42779)
                     /locus_tag="IW833_000764"
     CDS             complement(42144..42779)
                     /locus_tag="IW833_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ1306900.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(42813..43274)
                     /locus_tag="IW833_000765"
     CDS             complement(42813..43274)
                     /locus_tag="IW833_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAQ1306901.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(43404..43835)
                     /locus_tag="IW833_000766"
     CDS             complement(43404..43835)
                     /locus_tag="IW833_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1306902.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDK
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(43853..44173)
                     /locus_tag="IW833_000767"
     CDS             complement(43853..44173)
                     /locus_tag="IW833_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1306903.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            44472..45248
                     /locus_tag="IW833_000768"
     CDS             44472..45248
                     /locus_tag="IW833_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ1306904.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            45263..45466
                     /locus_tag="IW833_000769"
     CDS             45263..45466
                     /locus_tag="IW833_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1306905.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            45482..45820
                     /locus_tag="IW833_000770"
     CDS             45482..45820
                     /locus_tag="IW833_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ1306906.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            45807..45986
                     /locus_tag="IW833_000771"
     CDS             45807..45986
                     /locus_tag="IW833_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306907.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(46029..46499)
                     /locus_tag="IW833_000772"
     CDS             complement(46029..46499)
                     /locus_tag="IW833_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306908.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            46586..47284
                     /locus_tag="IW833_000773"
     CDS             46586..47284
                     /locus_tag="IW833_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ1306909.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            47462..47803
                     /locus_tag="IW833_000774"
     CDS             47462..47803
                     /locus_tag="IW833_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306910.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            47796..48467
                     /locus_tag="IW833_000775"
     CDS             47796..48467
                     /locus_tag="IW833_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ1306911.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            48473..49159
                     /locus_tag="IW833_000776"
     CDS             48473..49159
                     /locus_tag="IW833_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306912.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            49162..49911
                     /locus_tag="IW833_000777"
     CDS             49162..49911
                     /locus_tag="IW833_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1306913.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            49923..50192
                     /locus_tag="IW833_000778"
     CDS             49923..50192
                     /locus_tag="IW833_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306914.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            50354..50656
                     /locus_tag="IW833_000779"
     CDS             50354..50656
                     /locus_tag="IW833_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306915.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            50653..50814
                     /locus_tag="IW833_000780"
     CDS             50653..50814
                     /locus_tag="IW833_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1306916.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            50811..51116
                     /locus_tag="IW833_000781"
     CDS             50811..51116
                     /locus_tag="IW833_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306917.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            51116..51472
                     /locus_tag="IW833_000782"
     CDS             51116..51472
                     /locus_tag="IW833_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ1306918.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            51432..51677
                     /locus_tag="IW833_000783"
     CDS             51432..51677
                     /locus_tag="IW833_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306919.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            51674..52093
                     /locus_tag="IW833_000784"
     CDS             51674..52093
                     /locus_tag="IW833_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ1306920.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            52090..>52640
                     /locus_tag="IW833_000785"
     CDS             52090..>52640
                     /locus_tag="IW833_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ1306921.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVV"
BASE COUNT        17789 a         8801 c        10970 g        15080 t
ORIGIN      
        1 aattagaagc tggggcgtaa gtcctagctt ttttctatga atcatcataa acctgttata
       61 aatcgggaaa attctatggt agaatgaaaa cacaaaaaat atctgggagt ggttagatgg
      121 cagaattgat taacggaaaa gaattagctg aaaaaatgca ggcagagatt gctgaaaaaa
      181 tccaaaaatt gaaagataat ggtatccatc ccggattagt cgttttattg gtaggggaaa
      241 atccagccag ccaaatctat gtaagaaata aagaacgtgt agcaaaagaa atcggaatcc
      301 attcattggt agagcgttat cctgagacga tttctgaaga cgaactactt gcagaaattg
      361 aaaaatacaa tcaagatcct agttttcatg gtatattggt ccaattacca ttgccaaagc
      421 atatagatga agaaaagatc ctgttagcta tccgccctga aaaagacgta gacggttttc
      481 atccgatgaa tttaggcaga ctccttgcgg gtaaaccgga caagattcct tgtactccat
      541 acggcatcat gaaaatgttt gaagcttatt ctatcgatcc ggcgggtaaa cgagcagtag
      601 ttatcggacg aagcaatatc gtcggtaaac cgatggctca gctactcttg atggcagatg
      661 caactgttac tgttgctcat tctaaaaccg aaaatctggc tgaactggca caagaagcag
      721 atattttagt cgtagcaata ggacgtggac attttgtcac aaaagaattc gtaaaaccag
      781 gtgctgtagt gatagatgta gggatgaatc gtgatgctaa cggtaagctg atcggtgatg
      841 tgaaatttga tgaagtggag cctattgcag gctggatcac tcctgtgcca aagggtgtag
      901 ggccaatgac tatcacgatg ctgatgtatc aaaccgtaaa atctgccgaa gcttttggag
      961 caggtgaata gatggcacaa gaatatttaa ctgtcactac attaacgaag tatttgaaaa
     1021 gaaaatttga tgccgatcct tatttagaac gcgtatattt aacaggcgaa atatcgaatt
     1081 tccgtttacg tgccaatgcc catcaatatt tcagcttaaa agatgaccat gcaaaaatat
     1141 ctgctattat gttcaaagga gcattccaaa aattacgatt ccagccaaaa gaaggaatga
     1201 aagtccttgt cgttgggcgt atttcattat atgaagcggg aggttcttat caaatctatg
     1261 tagagcacat ggagcctgat ggaataggtg ctttatatca agaattggaa gaacgaaaag
     1321 aaaagctgat gaaggaaggc ctttttcaag ggcctaaaaa aatgctgcca cgtttcccaa
     1381 agcgaatcgc ggtccttacc agtccaagcg gagcggttat ccgcgatatc atcacgacgg
     1441 tgaaaagaag atatccaatt gtccaattgg ttttgttccc aacagttgta caaggaaacc
     1501 aagcagcgga caatgttgtc aggaatatcc agagagtaga agagatgggc aatttcgata
     1561 caatgatcat cggtcgtggg ggtggttcga ttgaggattt atggccgttt aatgaagagc
     1621 gggttgctcg ggcgatttat caagctaata cgccggtgat ttcatctgta ggacatgaaa
     1681 cagataccac gatcgctgat ttagtagcag atgttcgggc tgccactcca acagcagctg
     1741 cagaattagc tgttcctgtt ttgtcagaag agttgatgcg cattcaagaa cggcaagcta
     1801 gattgcagca ggcgtttgta cgccagatcc agcgcaaaca agaacggttc gagcgtgctc
     1861 gacgttccta tgtatttcgg cagcctgaaa gattgtatga aggtcagacc atcaaattag
     1921 accagttgaa acagcgatta tatcaagcta cacagcaaat ttatcatgcg aaagaaaaac
     1981 aaacgatcgc attaagccac caattggaac aagtggctcc gctttatcga gttaaagcag
     2041 ctcgccaaga aactgcttat ctagaaaaac gcttagaaga gaaaatccaa caatatatga
     2101 aacaacagca gcagcgtttt gctcaagctg ttcaatctct tgatttgttg agtccattga
     2161 aaatcatggg aagaggctac agctatacga cgttaaatga tcaagtagta aaaagtgtca
     2221 ccgagattca tcaaggggat ttgctgacga tccattatca agatggaatc gttaaaggac
     2281 aagtacaaga gatagacaaa ggagaataaa aatatggaaa atccaacttt tgaagagtca
     2341 ctgcaagaat tagaaaaaat tgtcatgcag ttagaacaag gagacgttcc attagaatca
     2401 gctctagatt cgtttaaacg aggaatggaa ttaagcaaac attgccaaga tacattagct
     2461 aaagcagaag agaccttgac gaaaatgatg acagaatcaa atgaagaagt tgattttgac
     2521 ggaaacgagg aaagctgaga tgagattaac tgacttttca gctattcatc tacctgctgt
     2581 agaaaaagaa atcttgtctt ttttagatga gcatacaacg gatagaggac tctatgatgc
     2641 gatgagttat tcagtgaaag caggcgggaa aagaatacgt cccttattac ttttaacggc
     2701 cgttgcttcc tttgatgagc ctattgatgt tcccgtctat caagtagcag ctgctttaga
     2761 aatggtgcat acgtattcct tgatccacga tgatttgcct gcgatggaca atgatgatct
     2821 tcgtagagga aaacctacca atcataaagt attcggcgaa gcattagcta tattggccgg
     2881 agatggtttg cttacaggag cattccagct aatcagtatg gcacacttag gtaattctcc
     2941 taagcttctg ctgcttcagc aattagctgt ttgtgcggga tcacaaggta tggttgctgg
     3001 gcaagctgca gatatcgagg gcgaatcgaa aaagctttct ttagaagaat tagcatttat
     3061 tcatgaaaga aagacagggc atttgattcg ttatgccttg ctggctggtg gcatattagc
     3121 gaagcaacct gaagaaatct tattgctact tcaacgatta gccgaacatt taggtttagc
     3181 ttttcagata agagatgatc tactcgatgt gattggtaca acgaaaacat tagggaaaac
     3241 agctggtaaa gatgaacgta tggaaaaaaa tacttacccg cgtttattag gcctagaaaa
     3301 gacacgtgag gcgttggaga ttgagctgtt atccgccaac aagatcatag acaaattaga
     3361 agaggacgtt ttttcttttg atggcgggct gatgcgccag atgatcaagc aatttgatgt
     3421 ggagaaatga gggaaagaat ggaaaaagaa cgcgtagatg tattagcggt caaacaagga
     3481 ttatttgaaa caagagaaca agccaaacgt tcagtgatgg ctggtttgat ttacaacgaa
     3541 aaaaacgagc ggtttgataa accgggagaa aaaattccag tgagtagtga attaagagtc
     3601 aaaggtaaaa aacttcctta tgtttctcga gggggattaa aattagaaaa agcactcaaa
     3661 gcatttgatc tatctgttga aggaaaaaca ttgttagata ttggtgcatc cactggcgga
     3721 tttactgatg cagctttgca aaatggcgca aaaatgagtt atgctttaga cgttggctac
     3781 aatcagctag cttggaaaat aagacaagat cctagagtcg tcgtaatgga aagagtgaat
     3841 tttcgttatg caaaacccga agactttacc ttaggaattc cagaagttgc tgttatcgat
     3901 gtttctttta tttcactaaa actgatgctg cctccattac atgctatttt aaaagaagag
     3961 ggagaagtga ttgctttaat caaaccccaa ttcgaagcag gcagagaagc tgtagggaaa
     4021 aacggaattg ttcgtgaccc tcagacccat caaaaagttt tagaagacat tttgtcattt
     4081 gcggcacaag gtggatatga tgtgctggaa ttaagttatt ctccgatcac ggggggagaa
     4141 ggaaatatcg agttcttggc tcacttgagg aaagtacccg aaagcggaac aatcaattca
     4201 gctattaata tggcagaagt ggtttcaaat gcacacgaac agtttgacca taaataaatt
     4261 gtaactgtga aaatcaagtg agaagtatgc ggattcttct ttattatacc aaggaaataa
     4321 gctatcattg attaagaaga atatgctaag gagcgaagac gtaatgagaa aaaaagatcg
     4381 gcatcgtttg attactcgat tgttgagtga gcaagatatc cgaaagcagg aagaatttgt
     4441 tgaaatcctg aaaagcaaag gaatatctgt tactcaagct acgatttcca gagatataaa
     4501 ggaactgaaa ctgatcaagg taccggcttc agatggcggc taccgttata gcataccagc
     4561 tgaaacaaac gaagatgtaa atactaaatt ggaaaaacta ttaaaagacg ctttcgtggc
     4621 agttgaccaa atggaaaaat ttgttatttt gaaaacattg ccgggaaatg cttcggcagc
     4681 agcaaatttg attgataaac gttacaagaa agaattattt tcgattatca atgatgacga
     4741 taatgttttg atgattacac gaactgaaga ccaggcagtt ttattgaaga aagacttcct
     4801 gcattacctt taagttgagg agcgaatgtg atgctacaag aaatcagtat tactaacttt
     4861 gcgattattc cggaattgcg gctttctttt cacgaaggaa tgaccgcgct gaccggagag
     4921 actggtgcgg gtaaatccat tattatcgat gctttaggtc tgttagcagg aggaagaggt
     4981 tcaagcgatt atatacgtca aggagcagaa aaatgtgtgc ttgaagggtt gtttgaacta
     5041 ccaaaacaag aaggattctc tgaactgatg gttgaattag ggatcgaaac agatgaggac
     5101 aatctgattg ttcgacggga tatgtcctta actggaaaaa atgtttgtcg agtgaatggt
     5161 catattatca cattagctaa tctgagaaga atcggcagtt atttagtaga tatccaagga
     5221 caaaacgaac atcaggaact gctccagcct gaatcacatc ttgctttatt ggatcgtttt
     5281 ggagatgtag ctttccaaca gaagaaaaag agttatcagc aagaatatat ctcttatcgt
     5341 gagctggaaa aaagggtacg aaaagtgcag caaaatgaaa aatcctatgt tcagagaata
     5401 gatatgctcc atttccagca agaagaaata gctgctgctg atttgcaagt cggagaagaa
     5461 gagaaattaa aagaagaaag agaaaaactt agcaattatc aaaaaattgc tgatggatta
     5521 gctgcaggtt acggagcact gacgaataat gaacaaaaca gtgtagatgg tgttggcttg
     5581 gccgtttcag aactgcaagg gatcgcccat ctagatgtgg agtatgaagc aatttatgaa
     5641 aatatccaaa gtgcttacta tttgctgcaa gatgcaatcg gcgatatgag tcgtcagatt
     5701 gatttattgg agttagatga gagtcgttta gaagaagtta cgcaacgttt agaattaatc
     5761 cgacagttga aacgtaaata cggcgaatcc attgagtcaa tattggctta ctatgacgaa
     5821 atcaccgaag agctagcttc ttctgatttt tcagaaggtc aattggacaa aatgaaagag
     5881 gaactagagc aaaaagagct tctacttcag caacaagccg cagatcttca tgaagcgcgt
     5941 aaaaaaatcg caaaagaatt ggaaaaatcc attctgcatg aattgaaaag tctgtatatg
     6001 gaaaacactg agtttgaggt acgtttctta aaagaagaaa atagacaatt gaatcatgat
     6061 ggatttgacc aaatcgaatt ttacattacg accaatccag gagagccact gaagcctttg
     6121 gtgaaagtcg cttctggtgg cgaactgtct cggatgttgc ttgctttgaa aactattttt
     6181 tcttctgagc aaggtgtgac aagtattatt tttgatgaag tggatacagg tgtgagcgga
     6241 agagttgcac aggccatcgc agataaaatt tctcaaatat cgaaatactc tcaagttttg
     6301 tgcatcactc atctgcctca agttgctgca gtcgccgact atcaatatta tattgtgaaa
     6361 gcagtgattg atggacgtac gcaaacttct gtttcagagt tgaaaacaaa agaaagagaa
     6421 gaagaaattg cacgtatgct ggctggaagt gagattacaa aactcacact cgaacatgca
     6481 aaagaactat tgaaactcgc aaaaaggacc tgagccacct cgcagtggtt caggtccttt
     6541 tcagcagctg atagctatat tttattttta catgacactc attacagcag tcaatacaac
     6601 agatccaatc gttaaaatca gcattgtcca aatgacaaat tttgttactt tagcaaaagt
     6661 acttgtttgt tttttttcac gcattgtttt caacctcgtc attaattagt tgattcaagt
     6721 gtacaatctt ttttccattt ccacaagagg gcttttttat aaaaacttcc ctttttttaa
     6781 ataataggta gttacgatac aaataaataa tgttaataga actagccata taaaggcatc
     6841 atccctttca gcaataggta acttaatgtt cattccatag atactgccga cgattgtcgg
     6901 tatagttaaa ataatcgtta aagaagttaa aattttcata atattattca gattattcga
     6961 tacgattgct gaaaaagtct gattcatttg ttctactagt tttaattgaa tgcgagaagt
     7021 agtcattgcc tgctctgttt ccaccaatac atcacgtaaa tgatttttca aagcatgatt
     7081 ttcttggaaa agtttatttt gcgctagttc tttcaatgca tctaaattag cagaagtcgc
     7141 ttctctgaaa taaacgaggc ttttttggat atccatgatt tgataaagtt gtttgttttc
     7201 agtggatact tgaagtttgg cttccaaagt atcgcattga gattgaagcg tagctaaata
     7261 actgttgtaa gagagtgtga cctgccaaat gaattgaaag aagatattga gattaggcga
     7321 aatatcactt tttggaaagg tctggctaaa gactttcttt aaaaacagtg gttcatgatt
     7381 cgtaattgtg ataagcttct ttttgggcgt caaaataata gagagcgggt atgtatccac
     7441 ttgaaaataa ccgctaggac ttgttttagc aaaaggatat tgtaacaata ataacgctgg
     7501 ctttgtcaac tttgattgat tgaattcttc tgtacgggaa ttttctgcat cgtccaatat
     7561 ggaagtgatg taatctgacg gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg
     7621 attaggacgt tctacacaat accagatcgt ttggtcagtt tcctttccgg tattacgcag
     7681 tttctcatta tctaatgtaa aatatttgat catgattttc ctcctgttgc ctgtttcttt
     7741 tattataacg gatagggaat tggaatacat ggaatgtgtt tgtaagatat agaatataac
     7801 tgcataaatt taaataaaat gaaacagttg tgtaaagtat atacagtata cttgaagcaa
     7861 cgataaaaaa ggagtttacg ttcatgggca cattcacacc acttatcttg ttttggtata
     7921 tttacccggt aatcgttttg tttggttgcc aatttcttgt aaaattattt tcgttaaatc
     7981 gtcgttttaa aataaaagcc cctgatttgg cgattccttt tttgtggggc ggtatgcatg
     8041 cattgtctag aaatacaggg acaatttcta tcttaccgtt tttctttatt tctgttttgc
     8101 taatgggaat ttgtattgct gttttccaag catattatta tgaagaaatt atttatccgc
     8161 gttatttcaa gatgacttgg cgtttggttt ttctgttgac aatcgtcttg tacgctgtct
     8221 tgatcattgt gaacatactt tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt
     8281 ttaaaaggct ttttgcagct tgatttttaa aaaaatgttt atgaataaaa aaagttgttc
     8341 ccggatttcc cagaaaaggt ggtagaaagt gggggattgt ggtagactaa ttttagttag
     8401 tggaggaatg gggggctttc aatgttgatg ggtgaattcc aacataatat tgatgcaaaa
     8461 ggacgtttaa tcgtgccctc aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca
     8521 cgcggattgg atggctgtct tttcggctat ccgatgagcg aatgggaaaa tctggaagct
     8581 aaactgaacg aaatgccgtt ggctaaaaaa gacgcacgta cttttgtgcg attcttctat
     8641 tcggctgcca ctgaatgcga acttgataaa caaggacgaa tcaatatacc cagcacgtta
     8701 agaaattatg cagctttgac aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa
     8761 atttgggatg agacacgatg gcaagatttt tctgcagcag cagaagagaa ctttgacgaa
     8821 attgctgaaa gtatgattga tttcggacta taagaatagg aagaggctga ctaatgactg
     8881 aaacatttca acactataca gtcatgttga aagaaacggt agatggattg aacatcaaac
     8941 cggatggcgt ttatgtagac tgtacactcg gcggtgccgg acatagtgaa tatttattaa
     9001 gccaactaag ttctaaaggg catctgtatg cgtttgacca agatcaaaaa gcaatcgatc
     9061 acgccaagct gcgtttagct tcttatgtcg aacaaggtat ggtcacattt atcaaagcga
     9121 acttcagaga attagaagaa cgcttgagtg agcatgtaga taaggtagac ggcattttgt
     9181 atgatctagg ggtttcctct cctcagctag atgaagcaga acgtggattt agttatcatc
     9241 aagatgcacc gttggatatg cgcatggatc agtcagctcc tttatccgct tatcacgtaa
     9301 ttaatgaata ttcctaccat gaattagtaa agattttctt ccgctatggc gaggagaaat
     9361 tttccaaaca aattgctcgt gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa
     9421 ctggtgagct tgtcgaaatc attaaacaag tcatcccagc tcccgcaaga agaaaaggcg
     9481 ggcatcctgc aaaaagaatt tttcaagctg tgcggattgc agtaaatgac gaactcggtg
     9541 ccgaagaggc ctctttagaa caggcaatcg ggttattgaa agtcaatgga agaatcagcg
     9601 taatcacatt ccattcattg gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta
     9661 cggtacaaga tctaccaccg ggacttcctg tcatacctga cgaattccaa ccagaactga
     9721 aagtcattac tcgtaaacca atccttcctt cagaggaaga gctaacagaa aacaatcgat
     9781 cacgcagtgc aaagttacgt attgcagaaa aaataagaat gaaagaggag tagaatgatg
     9841 gcagaattaa agaaaatgca agattacgaa tatgatttac ctgaattggt ggatcagccg
     9901 gaacaacaaa tgcctgattc ttcttcgaat caagaaatca ttcttccaca atcaccggca
     9961 aaacgattaa agcagatttc tgctttggaa aaaattgctg tggcaagcgt ggcgattgct
    10021 attgttgctt tatgtgtttt aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa
    10081 gaaatcacgt caatacaaac agaaatcaat gataaaaagc aagaaaaaac gagtttagaa
    10141 caagaaaaaa acgaactttc tcgtactgaa cggattaaac aaatcgcaga agaaaaagga
    10201 ttgtctatta atgacgacaa tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt
    10261 cggcggttta tggaaaagaa aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc
    10321 attttatttg ctacgagtat tggattattc tttttatttg ccgtccgttt ctcgtatatc
    10381 gttattggag gtcatgttgc tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa
    10441 ggaagtgaag tagtaaaagc aaaaagagga acgatttatg atcgtaacgg tgtggcgctc
    10501 gcagaagatg cctcctctta ttcaattaaa gcgatactct caaaaacata tacctctggg
    10561 gataaaaaac tgtatgttga agaaaagaat tttgataaaa tagcagaaat cctgcataag
    10621 aatctttcta tagacaaaaa agatgcactg aatattttag aggacggcgc aaaaaaagag
    10681 ctgtatcaag ttgaatttgg tagctatggg aaaaatatct cccaagaaac caagcaaaac
    10741 atcgaagcgg acatgaaaaa agaaggtgtc gctggtcttt attttgtcga ccatcaggct
    10801 cggatgtatc caaatggcgt cttttcttct cattttattg gatacgcagt tccagacaaa
    10861 gatgagaatg gcttagttgg taaactaggt ttggagtctg cttacaatga tatcttgagc
    10921 ggtaaagatg gtaagattat ttatcaaaaa gataattttc aaaatcctct gccaggtaca
    10981 gtggcagaag aagaaaaagc tgtcgacgga caagatatct acacgacttt agacagcaga
    11041 ctacaaagtt atttagaaac actgatggat caagtaaatg aagaatatca gccagaagaa
    11101 ttgacagcag tcctgatgaa agcaaaaacc ggagaaatct tggcaatggg acaacgacct
    11161 actttcaatc ctgaaacaat ggaaggattg actggaaaag atgctatctg gcgtaatttc
    11221 ttggtacaag atagttatga accaggatca accatgaaag ttttcactac tgcagctgct
    11281 atcgaagaag gagaattcaa cgagaatgaa accttccaat ctggaaaaat ccaagtagaa
    11341 gatgctacga tcaatgacca tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct
    11401 ctttcatggt caagtaacgt cgggatggtc atactggaac agcgattagg cggacgatgg
    11461 tataactatt tacaaaaact gggatttgga caaagtactc attctggatt agatgatgaa
    11521 gtaaacggtg ctttacctac attaaatatt gtcgaccgag caatgagtgc ttatggacaa
    11581 gcggtaggtg tgacgaattt ccaaatgatg aaagctttca cttctattgc gaataacggt
    11641 acaatgattc aacctcgata tatcagcaaa gtggtggatc cccaaactgg agaagagcga
    11701 acaactcaaa cagaagttct agggcaacct ttttcaaaag aaacaacaga aaaagtccgt
    11761 gaatatatga gagatgttgt cgaaagcgaa aactacggta gtgcttatgg tgtatatagt
    11821 gtacctggat acaatgtatc ggcaaaaacc ggaacagccc aaatcgcttc tgatacaggg
    11881 ggatatcaaa caggagatac agcctacctg tattcgattg tggagatggt tccttctgaa
    11941 gatccagact atgtcttgta tctaacaatg aaacacccta agacatatga tcgaatggca
    12001 ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg attttaaaga aactgaagaa
    12061 gattcagata cagaaacaaa aacagaaaaa gtatccgtag ccgattatcg taatcttgaa
    12121 gcggacgttg cagcagctga tgctcaaaaa agtggactgc aacctgtagt gatcggtaat
    12181 ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc agttgatctc tggagaaaaa
    12241 ctgatcctat atactggtgg tgataagctg atgcctgacg taacaggatg gtcaaaagca
    12301 gatatcatga agctggggaa aatcctcggt attgaagtta gttttgatgg agatggttat
    12361 tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg aagacaaact tagttttact
    12421 ttagaagaat aagaagagaa ataggagaga aaagagcatg gaatggacac aagcattgat
    12481 tcccattgtc agcagttgtg caatgacaat tgccgcaatg ccgctattta ttggttattt
    12541 tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga ccaaagtggc acaatagcaa
    12601 agccggaact cctacaatgg gcggcttagt gtttctgatc ggctctattt tgacaggaat
    12661 ttgggttgga gcttggcaaa aacagctaac tcctactctt ttcatcttgc tttttgtatt
    12721 agcgttatac ggagtaatcg gctttcttga tgattttata aaaatattca agaaaagaaa
    12781 catgggactg aactcgaagc agaaattgat tggacagatt ctagggggaa tcatctttta
    12841 tcttgtttac cgttctgaag gttatccagg tgtattgaat ttcttcggac tagatctacc
    12901 tttaggaatc atttatggta ttttcgctct cttctggctt gttggatttt caaatgcagt
    12961 aaatctgaca gatggcatcg atggattagt tgctggctta ggaagtatct cttttgcagc
    13021 ttacggtcta attgcttggc atcagcatca gtatgatgta ttaatcattt gcttaagtgt
    13081 tttaggaggt ttactgggtt tcttcgtcta caacagaaaa cctgccaaga tttttatggg
    13141 agatgtcggt tcattagcgt tgggcggact tttagcggca atttctataa tgctaaacca
    13201 agaatggaca ttgcttttga ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct
    13261 tcaagttact tcctttaagc ttacaggaaa acgaattttt aaaatgtctc caattcatca
    13321 tcactttgaa atgtgcggat ggtcggagtg gaagattgat acagtttttt ggttgatcag
    13381 cattgtgact tcgttgatca cattatggat cgtttggtag gagatgacgg gaaatgaaaa
    13441 aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa
    13501 gtgcggccaa attattacat gaattgggcg cacttgtcac cgttaatgat ggaaaacctt
    13561 ttgacgaaaa tccggaagca caagagctgc tttctttagg gataaaagta ataacaggta
    13621 gtcatccaat cgaattgttg gatgaagaat tttcattgat ggtcaaaaat ccagggattc
    13681 cctacagtca tcctttagta gcaaaagccc aagaaatggg tattccggta attacggaag
    13741 ttgagctagc ttacgaagta gcggaatgcc caattattgg gattacagga acgaacggaa
    13801 aaacaacgac aacaacgatg acaggactac tactgaatgc aggagcagat caaggaattg
    13861 cacgcttagc gggtaatatt gggtatccag caagtggtgt agcgcaagaa gcaaaatcag
    13921 aagataaaat tgtgatggaa ttgtctagct tccaactcat gggaatcaca gatttccgcc
    13981 ctcatatagc ggtgattacc aatatctatg aagcacatat tgattatcat ggtacaagaa
    14041 aagaatatgt caaagctaaa tggaaccttc aaaaaaacat gacagaaaaa gattatctga
    14101 tccttaactg gaatcagtca gaactacaag aattagcgca aagaacaaaa gcacgcgtac
    14161 tgcctttctc gacaaaagaa gtgttggaag acggtgtgta tgctgacgat tactcaatat
    14221 actataaaaa agaaaaaata atggaaatat ctgaacttgg cgtaccaggt aagcataatg
    14281 tagaaaatgc actagctgct atttctgtag caaaattgta tggtatatca aatgaagcta
    14341 tacgagaaac gttgcatttt ttccatggcg taccacatcg tacgcaatat gtaggagaaa
    14401 tccaaggcag aaaattttat aatgattcaa aagcaaccaa tattttagcg actaaaatgg
    14461 ctctaagtgg atttgaaacg tctaaggtcg tgttattggc tggcggatta gacagaggaa
    14521 atacatttga tgaattaatt ccttcattaa aaggaatcaa agcaatggtc gtatttggcc
    14581 aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg catcgagaca atagtaacag
    14641 ccgattctgt agaacaagca gttcctttag cattagaaaa cagtacagat ggtgatgtag
    14701 ttttattatc accagcgaat gccagctggg atcaataccc aaattttgaa actagaggaa
    14761 acagattcat ggaagctgtg aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa
    14821 atgaaaattt tggttacagg cggaggaaca gggggacata tttatccagc tcttgccttt
    14881 gtcaattatg taaagactca agaaccgaat gctgaattca tgtatgtggg agcaaaacga
    14941 ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt tccatacctt ggagattcaa
    15001 ggatttaaac gaaaaatttc catgcataac atcaagacgg tccgattgtt tcttaagagt
    15061 atccgtcaag caaaaaaaat cctcagtgat tttcaaccag atatcgttat cggtactgga
    15121 ggatatgttt ccggagcggt agtgtacgct gcagccaaaa tgggtattcc aacagttatc
    15181 catgaacaaa acagtgtacc aggtgttacc aataaatttt taagccgata tgtggatcgg
    15241 atcgctcttt catttaaaga tgcggcttcc ttttttcctg aaaacaaagc agttcttgta
    15301 ggtaatcctc gagcacaaga agtagcagat actaaaaaat cagatatttt gaaaacattt
    15361 gatttggatc cagaaaagaa aacagtcttg atttttggcg gtagtcaagg agcacttaaa
    15421 atcaatcaag cagtatcagt ttttttaagt gaatatattc cttcttctta ccaagttttg
    15481 tatgcttcag gagatagata ttatgaagag atcctaaata aaataaatcc tgaaatacaa
    15541 aatattagtg tccagcctta tatcaaaaat atggcagagg tcatggcttc cagtgatctg
    15601 ctggtggggc gtgctggtgc aacttcgatc gcagaattca cagctctggg gttaccggca
    15661 gtgttggttc ccagcccata tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt
    15721 gttcacgcag gagcagcaaa aatgattgca gataatgaac taacaggtga atcactaagt
    15781 cagacagtca acgaaatcat gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct
    15841 aaagaacaag gaatccctga tgcttcaaaa cgtctttatg acttagtaaa acaaataatc
    15901 aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc
    15961 cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag
    16021 aacaagagat gaaaaaccaa gagccaaaaa aagaagacat aacattagat acaccccttt
    16081 cagattctga agaagaaaaa acggttcaag aggaacagca agaagaaagc cagtccgaga
    16141 aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa cggtggacca tataacggtt
    16201 cttttttgaa ccggctgccc aatttaaaaa gtcaacggca caaagtacta tttcgtcggt
    16261 tagctttgat catcactgta ttgggtatac cgctgatttt tttgatctat tatgtttctc
    16321 cttatagcaa attacaagca gttgcagtat caggcaataa gacagtcaac agccaagaaa
    16381 tcatctctga tacaaaattg tcattaggag aaaatgtttg gggacagtat tttcatcgat
    16441 ctacttatat cgatcgcctt aaaaaagcac agccaagaat cgaaacagca aatatacatt
    16501 tcaaagggat gaatgaattc gatttggatg taacagaata taaagagatt gctttgatcg
    16561 caaaaaacaa tcaatactac ccagtgattg aaaatggaac agtattagat gaaaaagtag
    16621 caaatcctac aaaaaatctc cctatcttgg aagagttcaa agactctgca aaaatcaaag
    16681 aattaacaaa gcaatacaat cagttatcat cagaattaca aaaagcaatt tctgaaatca
    16741 agtacacgcc aagagcaagc aataaaaatc tgatccaatt gaatatgaat gacggcaatc
    16801 aagttattgt aaatatcaat aatcttgcga accaaatgaa atattattcg caagtagcca
    16861 aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg tatcttctcg catccttacg
    16921 gtagcactca agaaacggaa gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa
    16981 atgcggatca aacaaacgaa tcaagtgaag aggcagaaac gcaaaaagca caaacacctg
    17041 gatcgtctag cacaaacgga tcatctgatt caaatgattc aagtgaaaca agtcaaatag
    17101 aagataatcc aagtacaact ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa
    17161 agtaagaatg tactttctaa aaaccagtaa tttatggtag aattggaagc agtgaatata
    17221 tctattaata agtatatttt aaaacggacg ctagttgttc gcttgtatat ggaatagtaa
    17281 gtaggaggga atcccattca tggcaaaaac aggaatgtat gtaggccttg atattggaac
    17341 gacatctgtt aaagttgtcg tggctgaata tatcgacagc caaatgaata ttattggagt
    17401 aggaaacgca aaatcagaag ggattaaccg aggcattatc gttgatatcg ataaaacggt
    17461 ccaagcgata caacgagcag tacgacaagc agaagaaaaa gcaggtatcc agatcaaagg
    17521 cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa aattgccagg gaatgattgc
    17581 agttaacgga gattcaaagg aaatcaccga tgaagatgtc agaaatgtgg cttcagctgc
    17641 ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca atattgccac aagattttac
    17701 tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg atcggcgtaa gattagaaat
    17761 gtacggtctg ttgtttacag gacctaaaac gatcatccat aacatacgta aatgcgtgga
    17821 aaatgccgga ttaatcgtca acgaaatggt gattactcct ttagcgctga ctgaatcaat
    17881 tctttctgac ggagaaaaag attttggtac gatcgtcatt gatatgggtg gcggccaaac
    17941 aacaacggct gttatgcatg ataagcaatt gaagtttacc aatcttgacc aagaaggtgg
    18001 cgaatttgtc actaaagata tctctattgt attaaatacg tcatttaaca atgcagaagc
    18061 gttgaaaata aactacgggg atgcataccc agaacggaca tcaccagatg aagaatttcc
    18121 agtagatgtg attggtcagt cagaaccagt gaaagtcgat gaacgctact tgtcagaaat
    18181 tatttcagct cgaatggaac agattttcaa caaagcaaaa gatgcattgg accaaatcga
    18241 agcattagag ttgccaggtg gcgtcgtatt gacaggcgga gctgctagtc ttcctggtgt
    18301 tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa ttgtatgttc caaatcacat
    18361 ggggctgaga aacccggtat ttacaaatgt aataagtatc gtggattatt cagcaaactt
    18421 gagtgaagtg tatcaattgg caaaaattgc tgttacgggt gaaacctcag cagctcgtca
    18481 aatggtagtt gaacaagaaa caacaaacac atatgagagc tatgaagcac ctgaagaaac
    18541 aatctacgat gagccggaac caaaagagtc cggagaaaat gtaaaaaata aaatcaaggg
    18601 tttctttaca aatatttttg attaattcat cctgggagga ataagatatg gaattctcaa
    18661 ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat cggagtcggt ggtggcggtg
    18721 gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg tgttgaattc attacagcca
    18781 acacagacgt gcaagcatta aaaaattcaa aagctgaaac agttatccaa ttaggcccca
    18841 aatatactcg tggtttaggt gctggttctc agccagaagt tggacaaaaa gcggcagaag
    18901 aaagtgaaca atccttacgc gaagctttag acggtgcaga tatgattttc atcactgcag
    18961 gaatgggtgg aggaaccggt acaggtgctg caccaatcgt tgcaggtatc gcgaaagaat
    19021 tgggcgcatt gactgttggt gtcgtgacac gtccattcac ttttgaagga ccgaaacgtg
    19081 gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa tgtggataca ttattaatta
    19141 tctcaaataa ccgtttatta gaagttgtag ataaaaagac accgatgctt gaagctttcc
    19201 gtgaagcaga taatgtttta cgacaaggtg tacaaggtat ctctgatttg atcacagcgc
    19261 caggttacgt taaccttgac tttgctgacg tgaaaacagt gatggaaaac caaggtacag
    19321 cattgatggg gatcggtgtg gctagtggag aagatcgcgt agtagaagca acgaaaaaag
    19381 caatctcctc tccattgctt gaaacatcta ttgatggagc tgaacaagta cttcttaaca
    19441 tcacaggcgg tttggatatg acattgttcg aagcacaaga tgcttcagat atcgtagcaa
    19501 atgcagcaac tggcgatgta aatatcattc taggtacatc tatcaatgaa gaaatgggcg
    19561 atgaaatccg tgtgacggtc atcgcaacag gtatcgatga atcaaaaaag gaacgcaagt
    19621 ctagccgacc agctagacaa gcacaaatgc aatcacctgc tcaaaagact gtattagata
    19681 tggatcaagc taaaccaact tcttcagaag aagaaaacag ttttggcgat tgggatatca
    19741 gacgtgaaca aaatgttcgt cctagagttg atgattccaa ttttgacaat atcgaaaaaa
    19801 aagaatttga tacattcaac cgcgaagaaa caaaatctca tggcgatgat gaattaaata
    19861 cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag agcacatgat tgctgacaac
    19921 ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc ttgttcatcg tgatccgaaa
    19981 gaagttacat tagtagctgt aacaaaatct gttgaatccg ctcaagctga agaattggtt
    20041 agagctggcg ttatgaattg tgcagaaaac cgagtagata aattattaga aaagaaacaa
    20101 gatttatttg attttcctga aataaagtgg catttaattg gtaatttaca gcgcagaaag
    20161 gtaaaattga ttgtaaatga aattgattac tttcatgcgt tggacagttt aagattagca
    20221 gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt ttattgaagt aaatgtttct
    20281 ggtgaggaat caaaacatgg aatttcacca gatgagcttt taccgtttgt ggaatcttta
    20341 gctgcctgca caaaaatcaa ggttgttggg ttgatgacta tggcgcctaa agaagcttct
    20401 gatgagcaaa tccataaagt gtttggtacg ctgaaagaat tgcgtgatct tgttcaagca
    20461 aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg ggatgagtca agactttcaa
    20521 attgcgattg aagaaggtgc aacatttatc cgtgtaggaa cagcgctatt caaagacgaa
    20581 taaaggagga acaaaaatgc cgcttttaaa cagaggagca attgctaatt ttttcggatt
    20641 agctgatgag gaagagtacg aataccaaga ttatcctgaa acaacaaaaa aggctgtaca
    20701 gccaactaaa aaaaatacaa cacagaatca atcaactcat tctgtatctg ctgcgaatac
    20761 tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg catgaaagta ttcagccggc
    20821 tgaaaagaaa aaagtaattg aactacatca atcacctaca aaaactgtag cgaaagaaag
    20881 aacagcttca agaacctcta cacaacctaa agcaaatctt gctggaaaaa tcactgtaat
    20941 ggaaccaaga gcttattctg aagctatggc gattgctaaa cgaatcattg ctggagaagc
    21001 aaccttagtg aattttcatt tagtagaaga aaaacaagcg agaagaatcg ttgattttct
    21061 aacaggaaca gtttatgcac tagatggaga tatcaaacgt gttggagatg agatttttct
    21121 ttgtacaccg actgggatgg agattgacag ttcaaccgct caatcttttg cagatgcgaa
    21181 tttgtttgat ctgtaagagg aggaactacg atttatacta ttgcactatt acttcattta
    21241 cttcaacggg cagcctattt ctatagcatt atcttgatgg tatatgcatt gctctcttgg
    21301 tttccaggcg ggtatcaatc agggctagga cgattattag ctaagatctg cgaaccgtat
    21361 atcagtttgt tcgatcgtct gcctttgagg attggaccct tggatttcac aattttaggg
    21421 gcaatcctga tcttgaactt agccgtacaa ggtttagcac agatcgttat atggataact
    21481 gtccatttag tttattgatt gaagcaacaa aatctttatt ctcagaatag ataggagggg
    21541 aaaattttgg atgcaaatgt ataccagcac tttcgggcag atgaacgtac atttatcgat
    21601 tcggtaggcg attggattga acaggttcaa ggacaatatg caccctatct gaccgaattt
    21661 cttgacccaa gacaatccta tattttagaa acactgattc gacaaagcag tgatttgcgt
    21721 ttcatgtttt atggcggata tgaacaggca gaaagaaaaa gatgtttgat tttccccgat
    21781 tattacgaac ctgtagagga agattttgaa acagcattgt atgaaatcca ttatcctagc
    21841 aaatttgcca gcctaagtca tgggaaaatc cttggaactt tagtgggaac ggggatcaaa
    21901 cgctcttact ttggcgatat catctcggac ggtgatcgct ggcaagtatt tattgcaaat
    21961 gaggtggatc attttgttgc tgcacaagta acaaagattg gaaaggttgc tgtacgcttg
    22021 gaaccaatca aatacacaga aatgattatg ccgaaagata gctggacaca agaaagagga
    22081 acagttagtt ccatgcgact agacagtgtg atctctgaga tttataatat ttccagacag
    22141 cggtccaagc agctgatcga agctggtaaa gtaaaagtga actggacaga aaatacaaga
    22201 cctgattttg tacttgaact attagatatt atttcagtca gaggttatgg cagagtccag
    22261 atccaagaac ttgaggggaa gacgaaaaaa gaaaagtaca gggtactgtt cggtgtatta
    22321 cgtaaataaa ataaaaaggt ggtttactca tggctttaac tccattagat atccaaaata
    22381 agacattccc gacaaaaatg cggggatata atcaagacga agtagatgat ttcttagatt
    22441 tagttgttcg tgactatgag gaattgactc aaagaaatcg agaattagaa aaagcagtca
    22501 aacattctga agaaaaactt gagtacttca atgaattaaa agatgctttg aaccaatcaa
    22561 tcatcgtagc tcaagatacg gctgataaag taaaaacaag tgcaagcaag gaatccgaag
    22621 tgatcgttac ttctgcacaa aacaaagcag atgagttagt tgcgaacgct gaaaaacgtg
    22681 cacatcaatt aacaactgat gcagaagaaa aagcaagaaa aatcttgaca gatgcaacag
    22741 aaaaagcaag acagcttgcg acagaaactg aagatcttaa gaaaaaaaca cgagtattcc
    22801 atcaacgtat cagcttgatg cttgagtctc aattagaaca agtgaaaagt ccggaatggg
    22861 atgaaatttt acaaccattc tccagctacg tgactgacag ccatgaagtg atcaaagaag
    22921 ttttagcaaa acagcttgac aatgaaaatg atactgatgt aaactcagac acaggagtca
    22981 tagaagcacc tatgacggca tttgatacac aggctattga tatctcgatc gtgcctggtg
    23041 aaaatgaaaa cgagtagaac agaaaagcac tttgttattc ttaaagcgag ccgatgagag
    23101 tggaagttcg gtaagacact gcatggtgaa agatcctcta cttgagacct gcttttccga
    23161 aattagagta agaagcagcg gctgacctcg ttattggtca cagagagttt gcactagata
    23221 gatgcaacta aatttgggtg gtaccacggc atgttcgtcc cttgcggctg aacatgcttt
    23281 tttgtgtccg gaaaacaaat aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc
    23341 aattaggaaa aacaaaattc cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc
    23401 aaaaagaatg ggaagaaaat cgaatctacc aaaaaagaca agagttgaac gaaggaaaac
    23461 ctagctttgt tcttcatgat ggacctccat atgccaatgg taatatccat ttaggacatt
    23521 cattgaataa aatcagtaaa gacattatca ttcgttcaaa atcaatgtct ggctatcgtg
    23581 caccatatgt tccaggatgg gatacacatg gattgccgat tgaacaagta ttggctaata
    23641 aaggcgtgaa acgtaaagaa atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg
    23701 cactttccca agtagataag caacgagcag atttcaaacg cctaggagtt gcaggagatt
    23761 gggaacatcc ttatgttaca ttagatcctt cttatgaagc tgcacaagta cgcgtatttg
    23821 gtaaaatggc tgaaaaaggc tatatttaca aaggattgaa accaatttac tggtctccgt
    23881 caagtgagtc ttctttagcg gaagctgaaa ttgaatataa agatgtaaaa tctccatcta
    23941 tttttgtagc tttcaatgtc aaagacggca aagatattct tgatgaagat acagcttttg
    24001 tcatttggac gacaacacca tggacattgc cagcaaacca aggtattgct gtaaatccaa
    24061 aatttacgta tgtattggtg gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc
    24121 tagaaacagt ccaatcagaa atcggttggg aaaatgtaac agttctaaaa gaatttgctg
    24181 gacaagaaat ggaatatatg actgcaagtc atccattcta tgaacgtgaa tcacttgtga
    24241 ttttaggcga tcatgttact ttagacgctg gtactggatt agtccatact gctccaggac
    24301 atggggaaga tgactatatc gctggtagca aatataaact atctgtagtg tcacctgtag
    24361 actcaaaagg attgtttaca gaagaagcac ctggatttga aggtatcttt tatgacaaag
    24421 caaatcctat gatcactgat ctattaaaag aaaaaggagc tttgctgaag ctagatttct
    24481 ttactcatag ttatcctcac gattggcgga cgaaaaagcc tgtgatctat cgggcaactc
    24541 cacaatggtt tgcatctatc gataaattcc gtcagaatat tttggatgaa gtagaaaaag
    24601 tagagtgggt tatcccatgg ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg
    24661 attgggtaat ttcccgtcag cgtgcatggg gagtaccact tccgattttc tatgctgaaa
    24721 atggagaagc aatcatcaca ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac
    24781 atggatcaat tatctggttt gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc
    24841 acccaggttc tcctaatggc gaatttacaa aagaaaaaga tatcatggat gtctggtttg
    24901 attctggttc ttctcatgaa gctgttttac gtcagcgacc agaattatca ttcccagcag
    24961 atatgtattt agaaggatca gaccaatatc gtggctggtt caactcaagt atcacgacta
    25021 gtgtggctat caatggtgta gcaccataca aatcagttat ttctcaagga tttgctttag
    25081 atggtgaagg ccgtaagatg agtaaatctt taggaaacat cattgcacca gaaaaagtga
    25141 tcaaacaatt tggtgcagat atccttcgct tatgggtatc tagtgtagat tatgaagcag
    25201 atgttcgtgt ttctatggat atcttaagtc aagtggcaga agtatatcgt aaaatcagaa
    25261 atacgatgcg tttcttatta gcgaatacag aagatttcga tcctaaaaaa gatactgttt
    25321 cgtacaatga tttacgttct gtagacaaat atatgaccgt tcgattgaac caagtaatca
    25381 aagaaatccg agaagaaggg tacgacaaat ataacttcat gcatatttac cgtacagtta
    25441 tgaacttctt gacggttgat ctatcgtcat tctacctgga ttttgctaaa gatgtagttt
    25501 atattgaagc agaagatgat tatcaacgtc gttgcatgca aactgttttc tatcaaacag
    25561 ctgtagcttt gacaaaatta ttgactccta tcattccgca tacagcagaa gaaatctggt
    25621 cattcctgaa agaagaagag gagtacgttc agttaagcga gtttcctggt tatgaagagt
    25681 ttgcaaacca agaagaattg atggatacct ggactgcttt catggacttc cgtgataatg
    25741 tcttaaaagc attggaagaa gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa
    25801 aactaactgt ttatccaaaa caacaagttc gtgaaatgtt aaaagctttg gatgcagata
    25861 ttgcacaatt attgatcgtt tcaccagact actttgaagt tatggcagca gatgaacaag
    25921 tgccagataa cgctatggta tttgatgatg ttgctattac agtggaaaaa gcagacgggg
    25981 aaacttgcga tcgctgtcgc caagttcgta aagatgtagg agtagatgaa aaacttcctc
    26041 atctatgcgg tcgttgtgca aaaatcgttg aagacttcta tcctgaagct gtagctgaag
    26101 gattcgaaga aaagtaaaag ggaaaagggg gtgtgacatg ttctttgtca cacccccttt
    26161 tgttatcaat cgtttaattt tccacgttca cgataccagt tatctggttg ccagatccat
    26221 tcgaatccgt ctttttcaag tagttcgaaa gctgctttag gtcccattga tccagctttg
    26281 tagttgggaa aatccggttg tgtttggtcc caagcttttc gaatgacatc cacgatatgc
    26341 caagattgag cgacttcttc ccaatgagag aagttcgttc catcaccatt caacgcatct
    26401 aatagtaatt tttcgtaagc ttctgggcta ttttcaacca tttctgcact atttctaaat
    26461 tccaatttga ttggttccgt ttcaaatcct tgccctactt ctttcccatt caaggataaa
    26521 gaaaatcctt cagttggctg aatatagatc gttagaacat taggtggaag tgtcgtatcg
    26581 tcacatggtt catcgacaga tgttttgaat acattaacag gaacttgttt gaatacgatg
    26641 ttgatccgag tccctttttc agtcaaacgt ttgcctgtac gtacatagaa aggaacacct
    26701 gaccaacgga agttatcgat agtgaatttg cctgcgacga atgtttcagt ctctgattga
    26761 gcatccactt tgtcttcttc gcgatagccc ttgaattgta caccatccaa ttcaccttga
    26821 gcatattgcc cgcgaacgaa gttttctaac acttcttctt cgttataaat gcggatagct
    26881 ttcaatgctt tgatcttttc tgtacggatt tctttttcgg aaaatgctac tggcggttcc
    26941 attgcaagta atgctacaac ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca
    27001 ctatgatcgt agtagccccc gcgatcttct acaccgaggt tttcgccaaa agtaatttgg
    27061 atattgtcga tatagcgatt gttccattga gcctcaaaaa tattattggc aaaacggata
    27121 gctgagatgt tttggatcat ttcttttcct aaataatgat caatcctgaa aatatcttgt
    27181 tctgggaata cttctctgat ttgctggttc aattcaaaag cagactggta atcagaaccg
    27241 aacggttttt ctatgatcaa acgattgaag ccattttttg tcataatatt ttgtgatttc
    27301 aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag ccaaataata gacacggtta
    27361 ccgtttaatt gatagtgtcc atctaatcgg tcagacaatt cttttaatgt ctgataatgc
    27421 gttgaatcat ttacatcatg ggattgatag taaaaatggc ttgcaaaggt agttgcttct
    27481 tcatctgaag gattcaatgc acggattgtt tctttgacaa cttcacggta gtgctcatcg
    27541 ctccagggac ggcgagcggt tccgattaca gcaaaatgct cgcctaaatt tccttttctg
    27601 taaaggcgga acaaagacgg gtacaattta cgctgggcta ggtcacctgt accgccgaat
    27661 atcgtaaaca atacattttt ttcattagac atgtggatac actcctttat attttatcga
    27721 caaaaatcgt gctggccgcc ttaaagctga cggggatttt ctttctatca ttttggatag
    27781 tgatcggtcc ttcataagct gcgatgtcaa cgatctcata ttcttcgttg atattcaggt
    27841 caatagtgac taagtaatcc aacaattctt tttcatcaag cactcgtgcg atacgcactt
    27901 ttgtaccgac aggataggac tccaatgttt gtcggcgttc ttcatggaca acttcctctt
    27961 ttttaggaat cgatcctcca tgaggacaat gttcaggatg attcaaatag ttttctaaat
    28021 gatctgcaag atgttccgaa gtcacatgtt ccaatacttc agcatcatca tggacttcat
    28081 tccagctata gtctaaatgt tcaactaaaa aaacttccca taagcgatgt ttgcgaatca
    28141 gactgctagc tttttgcagt ccgcttgcag tcaattgtac accttgataa ggggaatgtt
    28201 ctacaagctc ttctttcaca agtttagaaa tcatttcact gacagatgca gctgagacat
    28261 ctagactaga tacgatttgt ttattgttga ctttcgttgt atctccacct agttctaaaa
    28321 tgattttcaa ataatcttct cgatttggtg tcatattcga tcatcctttc atggtaaaaa
    28381 accatcatta gtaagtgtaa caaaaaaaac gttaattgac tatttttcta aataaaaaac
    28441 aaaggatttt tagacgtttt tacaacaaaa aacgaaagat gatagagaag actcattctt
    28501 tcgtttataa aggatatata tatacattgg gagggatcta tttttcagat aacaacttat
    28561 atgaagtgtc tgcagattcg tcataaagag tttgttcggg ataagaaaag ataagataaa
    28621 aaccatactg atcacgtacg actaaacgat attcatctat ttctaccaca gatgtgccaa
    28681 gagactgacc gttgatcaaa aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct
    28741 ggctatcact aaagaaccat gtaccttgat actttgagag tccttctgac ttttccgctt
    28801 gttttttctt tttcaagtaa ataaaacttg agagaacggt aatgagtgaa ccaagaaaat
    28861 agatcatttt ttttatcacc aaatcactcc tttatgagaa aataatcgca tagacatatt
    28921 ctttcttctt tatttcattg taccgaataa aagctaaaat ttctctaaga ctttcgactg
    28981 gtttttttac tgtttttcta caaattatga taggatacat agtaggaaga aaggtgacat
    29041 aatttgaagt atcagcaagt ggaaatagct ttttttattg accgcccgaa tcggtttata
    29101 gcacattgta tgaaaaaaga cggagaaata attattgcgc atgtcaaaaa tacaggtaga
    29161 ggaaaagagg tcctgatacc aggcgctgaa gtggctattg tatttgctcc tggacctaaa
    29221 cggaaaaccg cctatgatct gattgctgta aaaaagaagg gtgactggtt taatatcgat
    29281 agtcaattgc ctaatcggct ggcaattgat ggtattttag acggcacgat ccttttacca
    29341 ggattaactg gtccatttac ggtatacaag cgtgaagtaa cttatcttca ctccaaattt
    29401 gatatttatt tagagacggt ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca
    29461 cttgaaaata aagcgattgg tgcttttcct gatgcgccca cactcagagg attgaaacat
    29521 ataggagaat taacgtatgc cgcacaagat ggttatgccg cttatgtctt gtttattgct
    29581 cagttcgaac atcttcatct ggcaacgatc catgaagaaa tgcaaccagc actagctgat
    29641 atggttcgac atgcgcagca aagcggagtt caaatcctcg cctataattg tcaagtatcg
    29701 gcagatgagg taactgtcaa gcaggccatt ccatttgatg tagatatccc ttttgaagat
    29761 cctatacaat agaaagaagg taaaataatg atcaacttag gtattatcgg aacaaattgg
    29821 attactcatc aatttgtcca agcagcttta agcacaaaaa aatatgaatt gacagcggta
    29881 tattctagac atttggaaaa agcacaggaa tttggttctc attatgaagg ggatatcgaa
    29941 ttcgcaacag atttgaagac ttttttcaat attactcata tggacaccat ttatattgct
    30001 tctccgaata gtcttcactt tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg
    30061 atagttgaaa agccagcttt ttcgacgcca aaagaaatgg atgaaatcat ccatttggca
    30121 aatgaacaca atgtgtattt ttttgaagca gcaagaaata tccatgaatc aggttttaaa
    30181 aagattgccg attacttacc attaaaagac gagataatcg gtgcaaattt cagctatatg
    30241 aaatattctt cgcgctatga ccaagtatta gatggagaag aaccgaatat cttctcgact
    30301 catttctcag gtggtgcatt gatggattta ggtgtttacc ttgtatatgc agctgttgct
    30361 tggtttggaa tgccaaaaga agtccattat ttcccaagaa agatttctac tggtgtagat
    30421 ggtcttggat gggggatctt acgttatgag aattttgatg tatcgatcca gccaggtaaa
    30481 atcggtgatt cttatttacc gtcagagatt tattttgact caggaacgct agtgatgaat
    30541 ggagtcaatg caattgaaca agcacaattt catgatcgcg cgcataaaga aatcattgat
    30601 ttggcggtaa cagctgcaga aaatccaatg gcagatgaag cagaggattt tgccaatgtc
    30661 atggctcatc cgaaagatcc agcttgggga ttgttgtatg aagagtggat cgagctagct
    30721 agaaatgtga accaagtgat ttatgattta agaaaaaatg gcggcatcaa attcgatgca
    30781 gataatgagg aagattttta atgaacgaat ttgtacaagt atttcccgaa aattggcaga
    30841 aaaaatatga ggataaaggg tttatctcac cttctcttat tcaacgagca gtttttcagc
    30901 cactaagtaa taaacaaagt atagtagcgg tttctcctac aggttcaggt aaaacactgg
    30961 cttatctttg gcctctacta ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt
    31021 ttgcttcttc tcaagaacta gctattcaag tagcagatgt tgcacgtgaa tggggaaaag
    31081 acaaagagtt gaaagtccaa tctttggtgg gcggagccaa cgtcaaacgt cagattgagg
    31141 gtttgaagga aaaaccagaa atcttgattg gtacaccagg aagaatattg gaattgatga
    31201 aagcaaaaaa aataaaagca catcaagtga aaacaatggt atttgatgaa gcagaccagc
    31261 tatttgacgc tgggaacagc caaatcattg atcaaattct tcatcaagct ccaacagagt
    31321 atcagctagc ttttttttca gcgacagcag atcgttccct tgaaagcatt gaaaaaatca
    31381 ctgggaaaac gtttgagaca attgacgtga cagcagagga cgattctcga aaaggactga
    31441 gacattactt tttacgtgta ccgatacgaa agaaagatga ctacttgaga cgtctcacgc
    31501 atatagaaga ttttcatggt ctgattttct ttaatcaact gactgaatta ggcgtaatgg
    31561 aagaaaaatt gctgtataga ggtgtaccgg taggaagtct ggcctctgat cagaacaagc
    31621 tactccgaaa agcagcgcta gaacaattca aaaagaaaat ggtcagagca cttttgacca
    31681 cagatgtggc ggctagaggt ttagatatta caggtcttcc ttatgtagtg aatgcagaag
    31741 ttccgttaag tgaagaagct tatttgcatc gttcgggaag aactggtcga atgggtaatg
    31801 aaggaatagt aattacactc gtacaagata ataatctaag agatttaaaa cggcttttac
    31861 gtccatcaaa tataacactt gaagagattt ttttatacgg aggtaagctg caaactgagc
    31921 agccaaccaa agaagatgca gctcctgcag taaataaaaa gtatgcgaaa actttttctg
    31981 gaaaggagca gaacccagaa tataacaaag aaaaaataag aaaaaaagtt aaaaataaat
    32041 caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc gcctttttat gctatgatta
    32101 tgaaaacggg aacaaaattg gttgccagag gaggggttat gtgaaagaac gtcaagaaga
    32161 gatcttgcag ctattgacag aaaaccaaca aggattgaca gcaagtgatg tagctgagcg
    32221 cttaactatc gatcgaagca atgcaagccg atatttaagt gaactataca aggcccatca
    32281 tattgtaaaa accgctggcc gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc
    32341 tgatgaagtg catgtcgact cttctacgca agtgactttt gaaacactcg ttggagaaaa
    32401 tgattcttta aaagtcagca tacagcaggc aaaagctgcg attctttatc caccgcgggg
    32461 gttgcatacg attatttttg gagaaactgg tacagggaag tcgatgtttg ctgaatgtat
    32521 gtatcatttt gcaatcgatt cagagatgct ttctgcagat gcgccatttg tttctttcaa
    32581 ttgtgcggat tatgctcaaa atccgcaact gctctttgga cacatctttg ggatcaaaaa
    32641 aggggcttac acaggagcag ctcaagatag tcccggtcta atagccaaag cagacggcgg
    32701 tatattgttt ttagatgaga tccaccgttt accgccggaa gggcaggaaa tgctgtttac
    32761 tttcattgat aaaggtattt accgtccgct tggagaaagt gcacaagtac atgaagcatc
    32821 tgttcagatc attggggcga cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg
    32881 acggattcct atggcaatca ccttgccgaa tcttgcttca cgttcactgg atgagcgtta
    32941 tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat
    33001 cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt
    33061 gaaacgagac ttgaaactag tctgtgcaaa atctttttta cattaccgta ctcatcatga
    33121 gaaaaagcta atcattcgaa aagaagaact ttcccttcaa gtacaaaaag gattactgaa
    33181 aatcaaggaa ataccggaac gtctcgaccg attcatggac agcaaaagtc agtatttgac
    33241 atttgaacca ggatttgctg atgttgtctg gtcacaagat ccagaacgaa acatgcaggt
    33301 ctataacgat attgaggaaa aagtcacttc tctttccgaa accggtgtgg agaacattga
    33361 tctagagacc ctcatttcaa aagatgtgga tgcttatttc cagacatatg tcaaagaatt
    33421 gactaaaagt tcgattccca aagatctttt gccagatgaa atctggcggt taaccaatca
    33481 attgtatgat gtggctgaaa aacggttaga ccgtatttat aatgaaaaag ctcgttttgc
    33541 ttttgctttg catctgcaaa gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca
    33601 cccagatttg aataacgtac ggaaaaattt taagagggaa ttccaggtag caatcgatct
    33661 ttcaacgatc attgaagaag aagaacaagt tgagattcct tttgatgaaa tcggctttat
    33721 tagtatgttt ctttctatca atctaggaga aagtgagcct gttaaagaaa acaaagtaga
    33781 tgttctcata ttgatgcacg gcagacagac tgcttcaagc atgttggaga tggcacaaga
    33841 actattagga acaaaagtag ggaaagcttt taatatgcca ctggttatgg aagttcaaac
    33901 aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa gagcatttga cgaatggcct
    33961 actcttgtta acagatatgg gatcattaaa caccttcagt gatttgattt atgaagagac
    34021 tggcattcgg acgaaagcta tcacgatgac aagtaccatg attgtgatag aagcgatacg
    34081 gatggcaagt gttggaagaa gcttagaaga tatttatcaa aatatccagc tttcctttga
    34141 atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc
    34201 cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc aaattatatc agcgtattat
    34261 gccagtgatt aatcaaaata aggtagaact gatccagatg cagtttttag aacgtgaatc
    34321 attcaaaaaa cacatcgatg ccttattgga agaatatgag atcaaagcaa ttgcgggaac
    34381 agttgatgtt gattatcaaa atatcccatt tttttcagcc tatgacgttt ttgatgatga
    34441 aaaactaaat attttaaagc gtatagctga tgatgagatc ccgacagcta aaattgtcga
    34501 ttcacttgct ggatcattaa gcgatgtctt ttctattgat gaactgatcc gttcattgca
    34561 aaagacagtt cagcagatcc agaccgattt gtcgattatc gtggaacctg gagtagatac
    34621 tggaatcgtt atccatttag ccttcatgtt agacggattg atcaaacaag aaagccatcg
    34681 gcaatttgag aaactgtcta ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg
    34741 ggcaaacttg atggcactag aacgaaaata tcaggtaaaa attccagaag atgatatcgc
    34801 atttcttaca caaatgtttc ttgaaaataa aataaaaaaa tgaacacact aatcatactg
    34861 tttacacact aaacatctgt gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct
    34921 atttcagctc ttttttttat tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa
    34981 aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt ttgttcagca gggatgagta
    35041 cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga aaaagggatg gaagccgata
    35101 tttttgcggt ctctgcctca gatgcagata ataatttaga atctaagaat gtcgatgtat
    35161 tattattagg tcctcaagta cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta
    35221 aaggtattcc attagatgtc atcaatatgc aagactatgg catgatgaac ggagaaaaag
    35281 ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta tgcagttggg ggtataacaa
    35341 cgtggaagat caacaaaatc tagaagccat catggggtta atcatgtttg gcggtaatgc
    35401 aaagagcgat gcaatggaag cgattgcggc tgctaaaatc ggtgattttg agttagcgga
    35461 taggaaaatc gctgatgcag aagaatcact tatacaagcg catcatgcac aaacagacat
    35521 gttgacacaa gaagcacaag gaaatcatat gcaagtgaca ttattgactg ttcacagtca
    35581 agatcactta atgacatcta ttgcgtttac tgatttagca aaagaaatca tcgaacttta
    35641 tcgtcgcgta tacagcgaat aaatacaact tcttttttga acggattttt tattttgtta
    35701 taaggagaac tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat
    35761 caagttggat ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt
    35821 tagttgcttt gcgtgatgct tttatcggca cactgcccgc aacaatggcg ggatcagtcg
    35881 ctgtaatgat caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg
    35941 acagtttagc agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg
    36001 gttttgtctg gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt
    36061 ataatcttgc ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta
    36121 ccttgattca aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc
    36181 cttcaaatgt cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca
    36241 cactgtcagt tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt
    36301 ttacagttat gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca
    36361 acattacgat caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca
    36421 ttttaccagc gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg
    36481 tagctgatgg gcaactgatc attgatttgg tgcaaaaata tattgcagaa ccattcctag
    36541 gattatctca agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt
    36601 tctttggtat tcatggacca aatgtattgg caccagtgct agagggaatc tggggacaag
    36661 cacaactaat caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag
    36721 ccgtgttaga tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg
    36781 cttttgcatg gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt
    36841 tctctaaacg tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca
    36901 atatcaacga accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc
    36961 cattcttgat tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc
    37021 tagtcgctcc ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct
    37081 tagcaacagg agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt
    37141 tcttgatttg ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac
    37201 aacaataaga tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt
    37261 cgaggaggaa ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa
    37321 ttgttagaag gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc
    37381 aagctgttct ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa
    37441 ctaatcaaag cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa
    37501 atggtttaaa aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt
    37561 ggttctttga tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt
    37621 atggtcacag gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa
    37681 agagaagaag caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag
    37741 catcgttttt ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg
    37801 gcaaatcagt aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa
    37861 ataaccgggc tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta
    37921 catcgacaac gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga
    37981 cctgtgaggt tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg
    38041 atatgctttt aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag
    38101 atatgatcga agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat
    38161 gaagtaagct agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag
    38221 cttttttgta ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc
    38281 gattgaggaa atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag
    38341 gcgagaacgt tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga
    38401 ggatcggcta acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga
    38461 gtacctaatg gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg
    38521 gatcaatcag atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc
    38581 ttatctggcg ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga
    38641 taaaatcagt tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa
    38701 aataaagcgt atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat
    38761 gccggaaaac caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa
    38821 gcaaaaaggc aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata
    38881 tatcgtttat cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca
    38941 tgatgctttt tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt
    39001 tgaatattca tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc
    39061 ttctgtaatg atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga
    39121 aaattatcca tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta
    39181 tgcgaaatgg aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc
    39241 aaaagtagaa caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct
    39301 attattaggg tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa
    39361 agaatatgaa gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta
    39421 tttttgataa aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg
    39481 gcatttgaag gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt
    39541 tcttttgttt tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc
    39601 cgattttttt tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc
    39661 attggaaagg acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac
    39721 tagtggaaac gttttgccta acttataagc tgattttttt agcagataag cttgcaagta
    39781 aaaaacaaac aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag
    39841 caatcgcctc ctaagcgata ggtcgctggt tcaattccag tcaggaacgt ataaataacc
    39901 cctgtatcct ttgcggtaca ggggtttgtt ttgtatttgt gccaaaaatg tgccaaacta
    39961 gttatcattt ttatataaac tatcgaacac agtattgatt ttttctttat cctgatcttc
    40021 aagctctcga acaatatgaa gataagtaga cattgtagtt tctaaactgc tatgtcctaa
    40081 acgtttcgat acgctaagta tatttactcc ttgataaagt aaaatagatg catgtgtatg
    40141 ccttagtcca tgcaaagtaa attgcttttc aatccctaat tcttttaatt ttttgcgcaa
    40201 atatttgctg acggcgtttg atgaaactaa tccatttttt aagttgaaaa aaacaaaatt
    40261 atgagggttt ttgatttcaa aattctcgaa taattctttt tgattgattt taaacttttt
    40321 taagagatca atcgtatgtt tatcaatgga aattttccta tttgaagttt cgttttttgt
    40381 ttttccccat tcatttaatt tataattcca tgttttatta atagtgatga tttgatcttc
    40441 gaaatcgata tcttcccatg ttaatcctag aagttcagca aatcgcattc cagtggcacc
    40501 agcaactaac acgagcatag gagaagagta ctgcgcagaa aggttttctt ctacgagttt
    40561 cattaaagat ttaaattgat cataatctaa atatttatct tcttttggct tcaatgaatc
    40621 ttttcctttg attactgctt ttctagttgg atcaaacgga attaatcctt cttctacggc
    40681 atctttcaac gacgctctaa tatggttatt aaacttaata acggtagatt ttacatggtc
    40741 tttagcatac ttatttaaaa agcgttgata tcccactcta tctaaatcag aaatcaatac
    40801 cgctggcata tatttcttta tgttcattaa cgtatcttcg tattttctat aagtgatagg
    40861 agatactgtt ccttctttat aaagttgcat ccaatcctca aagtaatctg ataataatag
    40921 atttttgcga tcccctttaa ggcctttgtt caagttatat tctaattcgt tggcggcatc
    40981 ttttgcttct ccttttgtct taaatcctga ttttcttatc ttcgtgtatt ttccgtcatc
    41041 ttttttgtat gaaatttcgt attgccaact atttccacgt ttgactaatc ttgccataat
    41101 tgaatcagct ctctttctct gatacaatag gaactataaa gaagcccatt gtttgggttt
    41161 tattattttt tgcacatcct ttttgcttgc cggcgcaagg atgtgctatt tgtcgtttta
    41221 atggtataat tggatgtgta ttagatcatc caatcagagg tgtttattat gattaaagtg
    41281 atttttaaaa gaataactga acttccagag tttagattat ttgttgatta ttggtatgtc
    41341 tggtttttta ttgttgcatt gctgataggt ctagatgtca tattagaaag aaaacttaag
    41401 taagagttga gttaataaat tgaattccta aatgagatat tttgtagccc atattttgtt
    41461 tgccgacatc acctaatact atggagctaa tcgttttttc taacagacca aaatcagata
    41521 atctatttaa gctaccatct attagctctt gtgaatagcc agacatgctg atatttccag
    41581 tgaaattttc gtttccacga ctcattaagc atgctaacaa ttgaatctca atcgatgtta
    41641 attgattcag tacaagaaga taatactcct cgttatccca tgaagcgttg ttaattcgaa
    41701 gtaaacaatt cttataaaga tttttataca tatctatttt attttgtgcc tttgattttt
    41761 ctacctcgtc atttatccct tcgaatattc caatgagttg gtccttgttc tctaagttga
    41821 aaacatcttc tggaatttga tcgcgaatgt tttctaatga ttcgtttaaa tttttgtaga
    41881 atttctcgat gcgtttgaaa cgcttctcgt ttttagaccc aaaataaagt gactgcacaa
    41941 cgccaccaat ataaggaatt gctccaatac cgccttcaac tattaattca agatattctt
    42001 ttttggttag tttttcattg ctattatcca tagttacctc ctattttgtc atttccttta
    42061 caagcattgt gactcctgat ggcactcttt tgtataggtt ttattattgt gcacaatcta
    42121 actcttagtg gggcgtggtt catttatttt ttacttactt tactattaat catatcccat
    42181 tgagcatagg atttttctat gttcgatcct cttgaactaa tttctcctgt aaaagttact
    42241 ttatcgccct ttgtaaaggt atcagcattg tttaagtcct tcgcaaaaat tacatatgaa
    42301 actttaggtg ttgcagaaat atcattccaa cttttatcat cgtactcttt gtctgctata
    42361 atggctattc tagtagtcat tgattcgata acagttcctt caaaagtata tgttttacca
    42421 ctaatatatt tagtaaaagc tgtattttgc tcagtagctg gagtaataga ataataacta
    42481 tctaagaaag caccccagtc atgattattt gtctctaaaa attcttcaaa ttgagttagg
    42541 cttttattgc tgctgttaga tgtagtagtt tgagtatcag atgatgagct ttcctgtttg
    42601 ttattgtcat ttgcctcgtt tgatgaggaa agcgagaaac ctacggtagt aaaaataatc
    42661 ccgataataa ggagaatcat aggtgttttc ttatttgttt ttttgataaa tcttacaatt
    42721 aacatgatta aacctataat aaataaaaca ataccagtaa aaccaaatat accgtccatt
    42781 aatagatcct ctttctcttt tgatattgaa tattataaac caagcaattc ttttttctta
    42841 aaatcgaatt cttcttgatt aataatccct tcatctaaca actctttata ctttttaatt
    42901 tcatctgcac ttgaaatttg tgtttctttg gaattattat ctaatataga ttgttgcttc
    42961 tctatatatt tttttaaatt ttccatttca ttccaatatt tttttgtaaa cataacggtg
    43021 ttttcatctt tcgtggccgc taatatacct ccgcgacttt cgtttccgcc tagcaaggta
    43081 aattgtatat aaccattact catacctggt ttttttaatt gtacggctga tatattttta
    43141 aaggggatag ttttttctcc tttaatacct tgattaacta aatttataaa tccttttcta
    43201 ataattgaaa tagaatcatc agttacttta atcaaggttt ttcctggaga ttttattaaa
    43261 atctcttttt ccattttttc ttcctcattt ctgtgatatg attttttttg tagaagctca
    43321 tatatgagga aagagcccgt gttgcagcac gggctttttt aatgttttgc tgaaatcggt
    43381 ttttttaata attcttgatg tattcataca tattgccttg cgtaagaata ttttttctta
    43441 aaatagcatt ggcagacagc ataacaagtg tatctgagct tattctgaat agaataatac
    43501 tccataaatt tttcgagatt aaactgagat ttatcagtca gttcgttctc aatatagata
    43561 tttaataaaa ttagaatagc tattttatct gcttctgttt caaattttga gtgaaaagtt
    43621 gtagaagtat cgtacaaaac tgaaaattca aaatgagagg cgctgaaatg tgcaagttca
    43681 tgagataaat gaaaggcttc tgcagtttca ccgtatagat tttcattcaa aaaaatgatt
    43741 ctgggttttg gatagtagaa accaggctct ttcatctcca tataaactac tttcaaattg
    43801 tattcgctca acatttcttt caatttcaaa tacatacaaa ccatcactcc aactattcat
    43861 tttcctctaa agctttagca attgcaatcg ctttacgcat tgtctcctta gatatttctt
    43921 ttccatcaaa agaaaaaaca gtatcgtctt ctgataaatc cacatgttta ggggtttctc
    43981 tttcttctct acctagaagg tagtctacag agacatcgaa atagtcagca atccgactta
    44041 attcatctga attaggagta ttatttttcc actttgctag ataaccgtta gaatatccaa
    44101 aattaatttc taactgccgt atagatatct ttttcctctt cgccaagtct tttattattt
    44161 cataggtatt catcgataaa acaacctttc taaatgttta caaaaaaagt ttagaaaaat
    44221 acgcagaaat aatttgacta attctgagta atgagctata ctatatcttg taaacagatt
    44281 taacaactaa aaagacaaca aaaaacacta ttgatatgta aatgcagacc gccaagaaag
    44341 ctttaaaatc aatgttttta tgtcttattt aattatgctc ttagtataga gtattactca
    44401 gatactgtca attgaattta gaaaaaagtt gttaaatttg tttacgaata taaaagaaag
    44461 gaatgagaaa tatgaacaca ccgcaaattt ttaatttcga acaaaacgaa gttcggacaa
    44521 ttttagtaaa tgatgaacca tattttgtag gcaaagacgt tgcgagtgtt ttgggttatt
    44581 caaacactaa agatgctttg tcgcggcatg tagatttgga agataagatg gggtcgcgaa
    44641 tcacgacctc aggtcaatca agagagatga caatcatcaa cgaatctggt ttgtacagtt
    44701 taatcttaaa atcaaaactt ccctctgcca aaaaatttaa acgttgggta acaagtgaag
    44761 tgttgccagc aattagaaaa catggaggtt atctaactcc agaaaaagta gaagaagctt
    44821 tgcttaatcc agatacaatc attcaattag caactcaact aaaagaagaa agaactggaa
    44881 gattaatcgc agaacaaaag attgcagagt acgaacctaa aatctcctat ttagatagca
    44941 tattatcttc tacagattca gtaacaatta gtcagattgc agcagattat gggatgtctc
    45001 cacaacagat gaataaatta cttcataaac taggtattca gaaaaaagtc ggtaaccaat
    45061 ggttattgtg caaaaaacac atgaaccaag gatacacaaa atctcataca actgagatcc
    45121 cgaaagccga tggtggcact aaaattgtaa tgaataccaa atggacacag aaagggcgtc
    45181 tatttatcta cgaattacta aaaaaagaag gatattaccc tcaaatggat ttagaggaaa
    45241 ttggttagaa aggagtttta gtatgactga cattgcagaa atcactcaac gagatagaga
    45301 aaaaatcaaa gaatatgtcg aaagttcaaa gttcttaact tacaccatgc ttgctgaaag
    45361 atttggaatt agcaaaagct acttatcttt aattttaaac ggtaaaaaga cttctgcaga
    45421 agcaaacaga attatagatt cgattatcac tatgtacgaa ttgtaaaggg aggaacagct
    45481 aatgcaatat ctagaagcaa aaatcccaat tccagaaggc tatgtaatta tctcccaagt
    45541 ggattatgag gagttaaaaa aagctgatga tactggtaga tggatgacgt tgccagaagt
    45601 gctagaacgg attaacagaa aatatgattg gtttacttct agagttttaa agaacccaag
    45661 atatagaaat attatcgata tcgagaaaaa caaaaatgga tttgtctatt atccagttga
    45721 aggaagagac acatatctat ttttaagaag taaaacactt gaatttttag aaacaaattt
    45781 ttcggaaatc ttaaggaggc aagcggatgg gaaaatttaa tagagcgcta gtgttcagcg
    45841 caccgctaat catctacgct ttaggacttt ggggaagcag gcaagcgttg ataggaacga
    45901 ttgtttacat ggtttggatt tttatggggc ttgatgaagc tgaagctgag tacagagcga
    45961 aaaagccaac cgagggggct gactaaaaat gaatagaaaa gagaaactag aatgtatatt
    46021 actattactc agtttaattc tttcactaat ttctctattg ggaagtttgt atttttgata
    46081 tcaacaattt tgtggatttg ggttctattt agagtaaata tcatttttgt ttggtgatta
    46141 aggagtttat taaaattaat tttccctact tcaaatgcaa ggtaacaaga tatagcactt
    46201 ttaggaggaa tatttagcgg tacagcttca gagtatattc tattatcatc gactgatcct
    46261 cctttagcaa ttaatttctt aaatggagta gctgaccatc tgcggtttaa gttagcggat
    46321 gaaagttcta aattaactaa catagctggt tctgttgaat agttagaaat gattactttt
    46381 gtataaatca tatctgcagc gaagtaagaa gcattaagtt caacttctag ttgagggcgc
    46441 attcctttta tttttatccc tgttaaaaac gtactcaata taaaacttat tattgacatc
    46501 cattgaaaaa ttgttaagtg aaaaaaattc aaaataaaca ccaccagttt ttaactaaat
    46561 tataccaaaa aggagagaag aaataatgca agaattagta attttgaaaa ataaagaagc
    46621 tgtgactacg agcttacaag tggcagaaag cttcgagaaa aaacatcaac atgttttaag
    46681 agatattgat gcactaaaaa aagatgtgtc caattttgga cagatgtttg tagaaggtaa
    46741 tgaaccagat tcatatggca gaaatcgacg agttttcttc attagtagag atggattttt
    46801 cttgctggct atggggttta caggaaagaa agctatctac ttcaaacaaa aatacattga
    46861 agcattcaac gaaatggaag atgttattcg caagaatact gttcctcaaa caattgaaga
    46921 tatgatgatc tatcaattag aagaaatgaa agatgttaaa aaagatgttt ccatgcttaa
    46981 agatactatg cgaattagcg gacaacaaga gtttgaaatt aagcaaaaag gaaatatgaa
    47041 agttatggga gttctagggg gaaaagaaag ccgagcttat gaagaaatca gcaaaaaagt
    47101 attctcaaaa ttttggtctg aatttaaacg taccttttca atcccaagat atggcgagtt
    47161 acctcgtaag agattcgatg atgctgtttc atttattgaa atgtggttgc cagaaactgc
    47221 gatccgtatg gaaattgatc aactgaacag acaacagaga ctttttggtg atgacaatga
    47281 atagagctga agcgctaaga atagggacgg caattgctaa ttgctggtgg aaatactata
    47341 aaccaatcat cctaagccaa caacatattg acaagcaaaa agcatggcaa caaataaaaa
    47401 agtgactccg ccggcaagca aagagtcaca aagaaaacac atcataagga gattttagca
    47461 tatggaaaaa gaactttcca ctctagatca atatttgatt gatcctgatt ggggcaagcc
    47521 gaaaattgag gaaacaagtg gtcgaaaaat cagacgaaat cttttgacga atgaagaact
    47581 agcttgggat caagatgatt taggcaacca tgtaactatt tgggatcatg tttatcttat
    47641 ccatctatcg aagcattcga ataaacctga atatatttac gtcatcgaag atggcttgat
    47701 tgatgcgcta gaagagtacg acagagataa cttgattgat atctcttatt acggaccagg
    47761 taagaaatac attgctgaaa tggaggcaga atttgatgag tgaaatcaaa gggacaacga
    47821 actttgaaaa actttttagt cgtaagttaa ataaaattct caagaaaaaa ggaaattttg
    47881 attatttatc ttgggctcac gcgtgggaga ttatgaaaaa gaatgatcca caggcaacgg
    47941 taactattaa tgagtacaaa cactacaggg ttgtttctgg aactcatcaa gactttcttg
    48001 ttgaggaata taaacctttt cttatggatg aaactgggac ttatgtatct gtctcagtaa
    48061 cggttaaagg acacacggaa accgaattat ttcctgtttt agattatcga aaccaaccag
    48121 ttgttaaacc aaatgcaatg caaatcaata actcattgaa gcgatgcttt gtgaaagcat
    48181 tggctctaca cggactggga ttatatgtat ttcaagggga agatattcca acaccaccta
    48241 gaatcgatac aaagaaatta aacatgctag agacgattct agaagctttc aatgagcaga
    48301 tgggtaaaga tatgaccaaa accttaatcg aatatgttaa tgagcagaca gataaattag
    48361 ggctcttagc tgataacgtt gaaactattg aacagttaag ctatgagcaa tgtgccttga
    48421 tggagcgagc aatagcagct aagagaaaag aattagataa gaagtgatat gagtgtttaa
    48481 accattaatc gattcatatt cagcggttct gaaaaagttc aaaggaaaag acataagcgc
    48541 aaccatcaat gaggaagtga acattgatcg actaaagacg atgtatgacg gctacgatgg
    48601 tgatcgagtc attgaaattc gttttattga tcctagacgt ttcaccgtac agcaacgaaa
    48661 cttcatctat gcgctgatag gcgatatttt tatcgataca ggcatgccaa cggacttctg
    48721 gaaggaattc ttctactttc gttttgaagg tgtcacaggg cgcaaaataa gcctgaaaga
    48781 cgaatcgaat acaactgtga gtgatgccaa tgtcttagca aatatcatct tagatttcat
    48841 ctttgaacat catattcctt tcaaagaagg ctatgagatt ttacctgcga atcaagaata
    48901 ttacttctac aaatgcatca caaaaagagt ctgctgcatc tgtggcaaaa caggagctga
    48961 catcgatcac tttgacaaag cgctaggaag acgaaagcgc aaagaagttg atcatgcaga
    49021 gtacacattt gcagcactct gcagaatcca tcacacagag aagcacaaaa taggtgtgat
    49081 taatttcaaa aataaatatc aaatcaaagg gatcaaatta aaccagaaaa caatcaaaaa
    49141 gttaaatatt ggagggtaaa aatgacagaa catcgaagtt attatgcgat tataccagcc
    49201 aacgtaaggt acgacaaaag acttaaacca aatactaagt tgttatacgg agagataacg
    49261 gccttgtgta atgaaagagg cttttgttgg gcaggcaatg agtactttgc agatttatat
    49321 ggtgtgaata aagagaccat atcgcgatgg gtaagtgatt tgattaagtt tggatacttg
    49381 aatcgggaaa tcatttacaa agagggtacc aatcaaataa tcaataggta cctacgaatt
    49441 aatcaatacc ctattgacga aaaacgcaat acccctattg acgaaaaagt caaagataat
    49501 aatacatcta ttaataatac atttaataat acaaaagaat atataagaga gttaccgcct
    49561 tcgaaaaaat cgaaggctaa gcccgtccgt cataaatacg gagagtataa aaatgttctt
    49621 ttgtcagatg agcagatgga gaaactcaaa acagaattcc ctaatgatta ccaagagcga
    49681 atcgaacgac tgtcagagta ttgtgaatca tctggtaaga cttataaaaa ctatttggca
    49741 actattcgaa gttgggcaag gaaagaaaaa agtgaaccta agaacgcaag cagtggatac
    49801 aagcgcacag gaagacgaga gaagcttcct gaatgggcaa tcgaccaaga agcctatctc
    49861 aagaaaaaag cgctagaacg agctaataga caatcaaaag caccattcta agaggtggaa
    49921 aaatgaagat cgattatcta gaactaatta atgaaatagc gaattataaa aagggcgagg
    49981 aattagacgt cctgagagac gtatatgatc aactcgaaga agctggaatt gaaggaatta
    50041 agaatgatcg ttcgagttgg agtaagctca gatactattt cgcactttat atcgatacaa
    50101 cacaattaag aaatttagca tataccaaat tactatttgt tgattgtgtt aaaggcttgc
    50161 aaaaacatct taatgaactt gagcaggtgt aatcagatgg acctaaagac atttacagca
    50221 cagattgaac taatgcatca agaagcttta agacaaagtg tatcgtacga agacaagtgg
    50281 ctcaacacgt tccatggcgg acgtgagagc gcacttgatc aagtactcaa attactgaaa
    50341 ggagaatgtc gggatggata agaaagcggc aatgcagcga attatcgaat tgacttattc
    50401 agaagattgg caaaatgaca aagaagctgc ttcagaagtg atgaggcttg gaagagcgat
    50461 gtgggcagac aagagcaaca agccaagacc acgaaaaatc gcaatttggc acggtgacaa
    50521 acttctagtg atagggacag ctgaacagtt agcaagtctc acaggcttgc acgagaaaat
    50581 cgtgaggaaa agagcaaggt gtggctacac agacgctaag aagagaacgt ttagatacgt
    50641 ggaggaatca tcatgacaac agaagaagtg attcaaatgc gtattcgaag ccttcagcgt
    50701 gagattgacg atctggaacg aacaaaggca gtgatggtca atgaaacggc gagaaaggca
    50761 atcgatttgc acatagagaa tttaagaagg gaaatccatc gattggagga atgagcgtgg
    50821 ataagaaagc agcaatgaaa agaattgctg aattaaccaa gtcagaatct tggcaagaag
    50881 acaaagaaat agttgcagaa gtccaaaagc tcggtaaatc aatgtggact gaaaagccca
    50941 aacggagaac gccgagaaaa attgcaatct ggcatgatga ccgaatttta gtaacaggta
    51001 ctgctgaaca gttatctgaa attactggat taagcaaaaa cattatctgg gatagagcta
    51061 ggagcttatg gattgattca aaaggacgac agtttaggta tgtggaggag agataatgga
    51121 tctcattaca caatacagtg acatcatcct caagaaaatc atgatgaaga ttcagaaaga
    51181 taaaaaatca aaagaacgag ctgaattagt taagttagaa atggctgaaa caggagcagg
    51241 agtgcgaagt agcaggcatt ggaaagcagc agcaaacatt gaattttatt acaacgaaat
    51301 tcaaaaaggg ttcgatcaga tgcgtgagct ggatcggcaa acaaattgga gcaagaaact
    51361 tcatcaagat cgtttcaaat ttgtagagaa gtataaagaa atattagaag agtatttgag
    51421 gaggacagca aatgataaaa aaactcgttc aattcagcat ggatttatat gatatcgaat
    51481 caggagcaac actatctgtg gaatcggacc atctaatcat aaattttggt ggaaagcgcc
    51541 agattatttt gtgggtagtt gatgatgtac tgtttccaga aattgttcat gatttcgaag
    51601 aatcaaaagc ggttgagttt gaaatagtga aaaaagtaat ggaattgatt gaaaaatacg
    51661 aggaggacag cgaatgatac cgaagtttag agcgtgggat aaacgaaaga acgtaatgag
    51721 agatgtagcc gtcttgcatt ttactaaaaa cggcaaaaca aactttattg aatattggat
    51781 aaatcctacc gaattgaaat catatcacgt gcgaaacatc gacctcatgc aatccacagg
    51841 aatgaaagat aagaatggtg tggatatatt tgaaggggat atagtattag tcagcgtgcg
    51901 aaatggcttc gattacttag ataataaagt ctgtattgtc aaaaattcaa tagattattc
    51961 cggattagtt tgtgccactg ttgatgaaga cttagagtat caaattttta acacagagct
    52021 gtttgaagaa tacacgtacg aagtcaccgg aaatatatac gagaatagcg agttattgga
    52081 ggaacagcga tgaataaaca ggaagcaatc aaaaagttag aaagtattaa agcgatagga
    52141 aatgatgcaa tagctgcttg ctataacgag agtataaatt caggtattac gttaatgaaa
    52201 aaaatagacg aaccgcagaa accagttatt ccacaactcg tggccggttg gcttgagaaa
    52261 tctacggacc cttttacaaa agctgaaaaa atagcgtatt taatcaaatc taaagatggt
    52321 gattcatatt atttctgtga ttggtttgta cgagatggca tagtgacgca agagcaagga
    52381 gaagaattac tcgcttgggc aacaagacaa tcatatgaaa cactattgag cctatacaac
    52441 ggctacgagg ttgagaaaga gccgttatgg gcaataaaga atgccgatgg aaactatctt
    52501 actaaatgtg ctttatgggg aaaagatgga gtaaattata gttttgaatg caatccatct
    52561 catcgattgc ttttcactga taaagcaaca gcggatgctg cagcattgtt ggtgaatgga
    52621 acagtggaag aggtggtaga
//
LOCUS       DACEQH010000013        52636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.013, whole genome shotgun sequence.
ACCESSION   DACEQH010000013 DACEQH010000000
VERSION     DACEQH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.013"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            23..901
                     /locus_tag="IW833_000786"
     CDS             23..901
                     /locus_tag="IW833_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306922.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            1377..1493
                     /locus_tag="IW833_000787"
     CDS             1377..1493
                     /locus_tag="IW833_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1306923.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            2421..2759
                     /locus_tag="IW833_000788"
     CDS             2421..2759
                     /locus_tag="IW833_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306924.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            2853..3083
                     /locus_tag="IW833_000789"
     CDS             2853..3083
                     /locus_tag="IW833_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1306925.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            3483..3854
                     /locus_tag="IW833_000790"
     CDS             3483..3854
                     /locus_tag="IW833_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ1306926.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(4128..4820)
                     /locus_tag="IW833_000791"
     CDS             complement(4128..4820)
                     /locus_tag="IW833_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1306927.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(4823..5608)
                     /locus_tag="IW833_000792"
     CDS             complement(4823..5608)
                     /locus_tag="IW833_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1306928.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            5845..7245
                     /gene="lacG"
                     /locus_tag="IW833_000793"
     CDS             5845..7245
                     /gene="lacG"
                     /locus_tag="IW833_000793"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ1306929.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            7270..7587
                     /locus_tag="IW833_000794"
     CDS             7270..7587
                     /locus_tag="IW833_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1306930.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            7600..9273
                     /locus_tag="IW833_000795"
     CDS             7600..9273
                     /locus_tag="IW833_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1306931.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            9287..10918
                     /locus_tag="IW833_000796"
     CDS             9287..10918
                     /locus_tag="IW833_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ1306932.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(11409..12974)
                     /gene="guaA"
                     /locus_tag="IW833_000797"
     CDS             complement(11409..12974)
                     /gene="guaA"
                     /locus_tag="IW833_000797"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ1306933.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(13033..13956)
                     /locus_tag="IW833_000798"
     CDS             complement(13033..13956)
                     /locus_tag="IW833_000798"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ1306934.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            14152..14757
                     /locus_tag="IW833_000799"
     CDS             14152..14757
                     /locus_tag="IW833_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1306935.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            15065..16081
                     /locus_tag="IW833_000800"
     CDS             15065..16081
                     /locus_tag="IW833_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1306936.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            16222..17523
                     /locus_tag="IW833_000801"
     CDS             16222..17523
                     /locus_tag="IW833_000801"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ1306937.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            17569..18231
                     /gene="deoC"
                     /locus_tag="IW833_000802"
     CDS             17569..18231
                     /gene="deoC"
                     /locus_tag="IW833_000802"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ1306938.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIVETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            18252..18650
                     /locus_tag="IW833_000803"
     CDS             18252..18650
                     /locus_tag="IW833_000803"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ1306939.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            18716..19798
                     /locus_tag="IW833_000804"
     CDS             18716..19798
                     /locus_tag="IW833_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1306940.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            20010..21572
                     /locus_tag="IW833_000805"
     CDS             20010..21572
                     /locus_tag="IW833_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1306941.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            21565..22698
                     /locus_tag="IW833_000806"
     CDS             21565..22698
                     /locus_tag="IW833_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1306942.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            22698..23654
                     /locus_tag="IW833_000807"
     CDS             22698..23654
                     /locus_tag="IW833_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1306943.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            23753..24919
                     /gene="deoB"
                     /locus_tag="IW833_000808"
     CDS             23753..24919
                     /gene="deoB"
                     /locus_tag="IW833_000808"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ1306944.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            24943..25761
                     /locus_tag="IW833_000809"
     CDS             24943..25761
                     /locus_tag="IW833_000809"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1306945.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            25778..26485
                     /gene="deoD"
                     /locus_tag="IW833_000810"
     CDS             25778..26485
                     /gene="deoD"
                     /locus_tag="IW833_000810"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1306946.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(26783..27469)
                     /gene="gpmA"
                     /locus_tag="IW833_000811"
     CDS             complement(26783..27469)
                     /gene="gpmA"
                     /locus_tag="IW833_000811"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1306947.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(27641..28222)
                     /locus_tag="IW833_000812"
     CDS             complement(27641..28222)
                     /locus_tag="IW833_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1306948.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(28355..29038)
                     /gene="rpiA"
                     /locus_tag="IW833_000813"
     CDS             complement(28355..29038)
                     /gene="rpiA"
                     /locus_tag="IW833_000813"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ1306949.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            29357..29770
                     /gene="rpsL"
                     /locus_tag="IW833_000814"
     CDS             29357..29770
                     /gene="rpsL"
                     /locus_tag="IW833_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ1306950.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            29854..30324
                     /gene="rpsG"
                     /locus_tag="IW833_000815"
     CDS             29854..30324
                     /gene="rpsG"
                     /locus_tag="IW833_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ1306951.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            30418..32505
                     /gene="fusA"
                     /locus_tag="IW833_000816"
     CDS             30418..32505
                     /gene="fusA"
                     /locus_tag="IW833_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1306952.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            32677..33864
                     /gene="tuf"
                     /locus_tag="IW833_000817"
     CDS             32677..33864
                     /gene="tuf"
                     /locus_tag="IW833_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1306953.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            34271..34579
                     /gene="rpsJ"
                     /locus_tag="IW833_000818"
     CDS             34271..34579
                     /gene="rpsJ"
                     /locus_tag="IW833_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ1306954.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            34610..35239
                     /gene="rplC"
                     /locus_tag="IW833_000819"
     CDS             34610..35239
                     /gene="rplC"
                     /locus_tag="IW833_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ1306955.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            35267..35890
                     /gene="rplD"
                     /locus_tag="IW833_000820"
     CDS             35267..35890
                     /gene="rplD"
                     /locus_tag="IW833_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ1306956.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            35890..36180
                     /gene="rplW"
                     /locus_tag="IW833_000821"
     CDS             35890..36180
                     /gene="rplW"
                     /locus_tag="IW833_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ1306957.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            36219..37052
                     /gene="rplB"
                     /locus_tag="IW833_000822"
     CDS             36219..37052
                     /gene="rplB"
                     /locus_tag="IW833_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ1306958.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            37095..37373
                     /gene="rpsS"
                     /locus_tag="IW833_000823"
     CDS             37095..37373
                     /gene="rpsS"
                     /locus_tag="IW833_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ1306959.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            37396..37743
                     /gene="rplV"
                     /locus_tag="IW833_000824"
     CDS             37396..37743
                     /gene="rplV"
                     /locus_tag="IW833_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ1306960.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            37757..38413
                     /gene="rpsC"
                     /locus_tag="IW833_000825"
     CDS             37757..38413
                     /gene="rpsC"
                     /locus_tag="IW833_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ1306961.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            38416..38850
                     /gene="rplP"
                     /locus_tag="IW833_000826"
     CDS             38416..38850
                     /gene="rplP"
                     /locus_tag="IW833_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ1306962.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            38840..39028
                     /gene="rpmC"
                     /locus_tag="IW833_000827"
     CDS             38840..39028
                     /gene="rpmC"
                     /locus_tag="IW833_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ1306963.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            39053..39319
                     /gene="rpsQ"
                     /locus_tag="IW833_000828"
     CDS             39053..39319
                     /gene="rpsQ"
                     /locus_tag="IW833_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ1306964.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            39377..39745
                     /gene="rplN"
                     /locus_tag="IW833_000829"
     CDS             39377..39745
                     /gene="rplN"
                     /locus_tag="IW833_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ1306965.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            39782..40090
                     /gene="rplX"
                     /locus_tag="IW833_000830"
     CDS             39782..40090
                     /gene="rplX"
                     /locus_tag="IW833_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ1306966.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            40118..40657
                     /gene="rplE"
                     /locus_tag="IW833_000831"
     CDS             40118..40657
                     /gene="rplE"
                     /locus_tag="IW833_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ1306967.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            40676..40861
                     /locus_tag="IW833_000832"
     CDS             40676..40861
                     /locus_tag="IW833_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ1306968.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            40897..41295
                     /gene="rpsH"
                     /locus_tag="IW833_000833"
     CDS             40897..41295
                     /gene="rpsH"
                     /locus_tag="IW833_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ1306969.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            41327..41863
                     /gene="rplF"
                     /locus_tag="IW833_000834"
     CDS             41327..41863
                     /gene="rplF"
                     /locus_tag="IW833_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ1306970.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            42024..42380
                     /gene="rplR"
                     /locus_tag="IW833_000835"
     CDS             42024..42380
                     /gene="rplR"
                     /locus_tag="IW833_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ1306971.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            42401..42901
                     /gene="rpsE"
                     /locus_tag="IW833_000836"
     CDS             42401..42901
                     /gene="rpsE"
                     /locus_tag="IW833_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ1306972.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            42916..43095
                     /gene="rpmD"
                     /locus_tag="IW833_000837"
     CDS             42916..43095
                     /gene="rpmD"
                     /locus_tag="IW833_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ1306973.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            43139..43579
                     /gene="rplO"
                     /locus_tag="IW833_000838"
     CDS             43139..43579
                     /gene="rplO"
                     /locus_tag="IW833_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ1306974.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            43579..44874
                     /gene="secY"
                     /locus_tag="IW833_000839"
     CDS             43579..44874
                     /gene="secY"
                     /locus_tag="IW833_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ1306975.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            44936..45583
                     /locus_tag="IW833_000840"
     CDS             44936..45583
                     /locus_tag="IW833_000840"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ1306976.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            45774..45992
                     /gene="infA"
                     /locus_tag="IW833_000841"
     CDS             45774..45992
                     /gene="infA"
                     /locus_tag="IW833_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ1306977.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            46025..46141
                     /gene="rpmJ"
                     /locus_tag="IW833_000842"
     CDS             46025..46141
                     /gene="rpmJ"
                     /locus_tag="IW833_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ1306978.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            46159..46524
                     /gene="rpsM"
                     /locus_tag="IW833_000843"
     CDS             46159..46524
                     /gene="rpsM"
                     /locus_tag="IW833_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ1306979.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            46552..46941
                     /gene="rpsK"
                     /locus_tag="IW833_000844"
     CDS             46552..46941
                     /gene="rpsK"
                     /locus_tag="IW833_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ1306980.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            47021..47959
                     /locus_tag="IW833_000845"
     CDS             47021..47959
                     /locus_tag="IW833_000845"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ1306981.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            47987..48367
                     /gene="rplQ"
                     /locus_tag="IW833_000846"
     CDS             47987..48367
                     /gene="rplQ"
                     /locus_tag="IW833_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ1306982.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            48639..49478
                     /locus_tag="IW833_000847"
     CDS             48639..49478
                     /locus_tag="IW833_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1306983.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            49454..50323
                     /locus_tag="IW833_000848"
     CDS             49454..50323
                     /locus_tag="IW833_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1306984.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            50313..51110
                     /locus_tag="IW833_000849"
     CDS             50313..51110
                     /locus_tag="IW833_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1306985.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            51313..52059
                     /gene="truA"
                     /locus_tag="IW833_000850"
     CDS             51313..52059
                     /gene="truA"
                     /locus_tag="IW833_000850"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1306986.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT        16789 a         9480 c        11416 g        14951 t
ORIGIN      
        1 cggataatca gtcatttaaa caatgaggtt caaagatatg aaaaggggaa atttgatgaa
       61 gaaaataagc aatgaaatcc aaattctggc agaaacttta actcttccca aagtcgttat
      121 agagcaaatg attgtacaag caaaaaacat ccttgatgag gtgtttgagc aatatagtca
      181 tgaactaatg gatcctaata aagcaaaggc gactgcaaag aaaatagaca aagtctatga
      241 agaggaacct tttgcagcat tagctattta tctatatgca gcttctcaaa gttggaaaaa
      301 aatttatcaa ccattgaaga ttccaagaac tatctatttg gcaacaatga atgcttttac
      361 tagatttatt catgaacatt tccaagtaac aggaagctat cgctttgacc gtggtttttg
      421 gatttggcat tacactagtg gcttgatctt tcggattggt gaattggagt acgaaatgac
      481 atattttccg aaagggtaca cggatctaag attagaagga aagcagtgcc tttctcttca
      541 tattccttcc gatgcagatt tatctcagaa taagataaga gatagttacg aaagtgccat
      601 tcgttttttt gaactttatt ttcctgatta ccattaccaa gtgatgtaca cagatacttg
      661 gttactatca cccaatctta ctaaatggtt gaaaaaagag tcaaaaatct gtctttttgc
      721 agccgattat cgattattat cagttgatga acaggacgat agtggagtcc cttggatctt
      781 tggacgagta gatgcccaaa tacatgatta tccagaaagt acgagtttac agcgacaagc
      841 aaaagagcaa ttgcttgcag gcgagcacat tggctctggc ttaggtattc ttacttcata
      901 agtttagtac ttcgaaatag tttaaaagag cgtgagacaa aagtaaagaa cacttttgtc
      961 tcacgctcta aacacgtata aacggcggaa gcagaagcaa ctccttcgga aataagctga
     1021 aattcacaaa aatttgaaaa gcaattttcg tgaatttctt cttatttctt ggagttaaac
     1081 gcttctgtcc cagcctcttt tttagtgttc tatcgaacac acagtacgtt caataggtaa
     1141 actagctata acaatagata ttaaagttaa cggacaagag ggcctttttt aggtagctaa
     1201 aacgtaataa tgaaaattta tttccagaga taggagtcaa tcagctagtc tgatcgactg
     1261 ttttaacaag ttgactttca ttttaacttt tgataccatt taaaacacaa atcgtaattg
     1321 tttcgatttg tgttttttag catgactaca atcaaagaag aaagtgggtc gtccatatgg
     1381 aaaaaacaga tatctcaagt gcataccgcc gcttgaaaag cccaaatatc aaaacacgaa
     1441 aacgtgcgct gaagatcatc aaagatgtga aacggaatag tgggaaaagg taagaagaag
     1501 gaaacttaat taaaaaaata gccgaaaaac gtttttcggc tattttcaat aatcggtttt
     1561 tgtgtaaggt ttccgaaaga actgctgttg attttccatt tttaacatat gataaattgg
     1621 tagaatcttt cttttcaaat tgataccatt ccctaattcc agttatatag ttaactacag
     1681 aaccacaaag cacgtacgtt tataaaagta ttatctgtag ttatttttca attccctttc
     1741 aacctcttat tttgaccagt agtattgatc actttacctt ttaacacctt tgttcgtttt
     1801 tgaaacattt ttagtcaaga atggaaacgt aactacataa tcaaaggttt tgcgaaatat
     1861 ttcatgaaat atttcatgta gaagcgagca atacggttgc aaataaccat tatcgtttaa
     1921 taagcagtta cgaaagctcc caatatctct aacgttcagc tcactccatt tcttttcttc
     1981 aaaaaaatca gtgattcgta tctctttcta tttgtcgaaa aagttaactt taaaaagtaa
     2041 cgccgaaaaa atatgcaaaa aaataaaaaa gcagttacta ttgctagaat aacatcaagg
     2101 aataataagt tttggttttc agataatagt cgataagttg tgtattggaa ttttagatac
     2161 agtaataaag tatttttcat gaaattttgt gagaatcact attacaagta tctttaataa
     2221 aattaatggt taaggaggaa cttaaagtag aatgtaaatg aatagttgta gtcagtcgat
     2281 tgtgatacta gactcaaagt tgcaggaata agtttttcaa tttgtgattg gaagataaaa
     2341 aatgaaaaag aagggctttt ttttatactt tgttgattgt agtgtttatt ttccctctgt
     2401 ttagccaagt aacattttct atgactcaac cagtttcaag tgaggaacat gataaccttt
     2461 ttcgtgctac agaagagttt aagaaatatc aggaaaatat tgttccggag actccattag
     2521 ctagtgagcc aattactgat attgatggaa accaaatagg atggtatata cagtacgaag
     2581 ttatggtggt aaataatgac tcaatagtaa aaaatacagt aggcttagag ctaacaccga
     2641 ttgaaaaggt gagttcattg ttaacgtata tttatactga tgaaagtgac gagactgaga
     2701 tcattttaac ggattactca aaaattgcta ctgataaaaa gtatatattg tggatgtaga
     2761 cactagtgaa atactttatg atatagaaat tgatgaaaat ttactagatg tagcacaaga
     2821 aatcgatcat caaaagcagg aagctattcg atatgctggt agaaaaggga aaagaaaata
     2881 tttattacgt gaatgtagca aaagttagag aagatgaaaa tgagtggaaa gaattcaaga
     2941 gtcgctactc aattaactcg actccgacat ttacagttta cagagaagga tcgatagaaa
     3001 aaactgtttt ttggactaaa gaaagtggaa tgtctttagc tgaagtagaa gagtttttgg
     3061 attatgttag tatgcaacag taagggagtt cttattatga aaaattttga cacatattat
     3121 gttattgttt tttcgatatc tttgctgtta atattactaa ctttaatgca gtataaagag
     3181 tatccagagt attttatttt aacttttgta tatttagtaa gtagctacac acttctatat
     3241 aaattcagaa gttcttctag agtttctaaa gtttgtttct taattagctt attgattcta
     3301 taatattttt gatttatata ggaaattaaa aatattatag aatcaacact cagagaacgg
     3361 gttggttttt aatagattta ataagtactg ataatgaaat ttctcctggt tgggaaaacg
     3421 aagttggtta ccctgtagaa gaattttttc aagattactt tgcggagtat gccgatagga
     3481 taatgagaga ttacgtaaca gataaaactc aagaagtcat caatcaatcc cagtcattaa
     3541 atgaattatc tgataaggcg aaagaagaag cagttgccat tatttcttca gggattagtg
     3601 actatattga tatgagtggt gaaatcaatt atggcgataa agacaaaatt gtacgcatga
     3661 taaaagcttt tatcaaaact tttgatgtga aggcagatct taagtttaat gatacttacc
     3721 tagtaggaaa attaattaat atccttggga cagagaaaga tacggaaacg tttatttcct
     3781 ctttgtctat gttgtttaca gataagcatt tttcaactca gcttggtgga gaggaactag
     3841 tggaaatctt ttaaccatta cgcccagcat taataaaagt atatcaaaaa agtactcctg
     3901 atgattttta agattggttt gagaaataat gtgaaaacac tatttttaat gttaattata
     3961 gatagagatg aacgcgtcat atggttaaaa gaatgaaaaa gtcaaagata cactgctata
     4021 tttcactaag gatatagcag tgtatctttc atgtcgtttc actggcagtt ttttataaaa
     4081 ggaggtgtgc atttcagttg cttttctttg gtagttagtt gaatcgttta agaattaacg
     4141 gatgtggcag acaggtgctc tgtccaaaaa ccagtaaaat ctttgatggt tccatcttta
     4201 tttagctgtg cctgttcaaa tactgttttg tttttaccga atggatcaat tgcaataatt
     4261 gttccgatcc ccgcacgttt ggcagcaagt aatcctgagt aagcatcttc gataacaaga
     4321 caatcagccg gagctaagct cagtttgtct gccgctttaa gaaaaatatc cggttgcggt
     4381 ttcccaggga aagagccgtc gtcatagaca atcttgtccc agtcaaacca gcgggctaaa
     4441 tcgaaaatat caaaataaaa agcaacattc tctttgacag tagccgtggc aatcgttaat
     4501 ggaatctgtt ggtcagtcag ctggtcaaga gtcgcttcaa gcccttttgt caactgaagc
     4561 tcgttttcat tttgcaagca aagcatacga taatagcttt ctttttcttc tgacaatacg
     4621 gcgatctcag aggcggtcaa atgctctgaa atgaaatgtt tcaaaatctc cgtattcgtc
     4681 cgaccatgaa tattttttaa aatatcctcg tctgataaac catcttttgt atgatcatgt
     4741 atcatatgca gccaagcagc ttcatgcaga tgagagtcca aaaacattgt tccgttgaaa
     4801 tcaaatataa tccctttcat agttacgctc ctatagtggt attttcggtt gaatcagcaa
     4861 tttgcagatc gacatattgt gcatagtgct cttgaacctc ccgcgagatc tggcgatccg
     4921 taatgagcag atcgaaatca tgcaaattat aaaacacata aaaatcatct cgattgaatt
     4981 ttttattatc aacgagtaga tatttcttac gggaattatt tagagcgatc tgctgtgctt
     5041 ctccttcttc ggtactgtaa gtagagatcg cttcgttgtg aacgccattt gaactgataa
     5101 atgcttttgt aaactttagt cttcttaaat tgtcgttggt gatcggtccg acaaatgcgc
     5161 cggtattttt ccggaaatca ccgccaatca gtagaatttc tggtccatct gcttgagcaa
     5221 gaatatcaaa aactggataa ttatttgtaa tgatccgaat tttcttgttc agtaaatgcg
     5281 tcgccaatag ttcgattgtt gtacctggtc ctaagaaaat cgtttctccc tcgtgaatca
     5341 aggaggctgc tgtggcagca atttcttttt tttcttgaat ctgcagagtg gctttttcag
     5401 tgtgagaaag ttcttgatct aaagtgtagg aaagactttg agcccctcca tggacacgca
     5461 gcagcttgcc cgatttttct aattcatcta aatctctgcg aacggtcata tcagaaacat
     5521 ctaattgatt gatgatttca ttaacggtaa ggattccttt tttattgacc atttctacga
     5581 tagtaagcaa tctttctttt ttcaacattg tcttcacctc taatttaagg ataatctatc
     5641 atataaacgg acaacagtca atctaattcg aacaaaagta aacatttagg gtggttttta
     5701 tgtttgttac aatagatttt attgctgttt tctggttttt atgattattt gtgtttgttt
     5761 ttgttgacaa atgtttcgga tgcgtttaca atgcaatcag ttagaaagag cgaaaactga
     5821 ttatagattt gaggagagat gtacatgctt aaattaccag aagattttat ttttggcgga
     5881 gccacagcag cctatcaggt tgaaggagcc actaaggaag gtggcaaagg cgctgttgct
     5941 tgggatgact ttttagagga acaagggcga ttcagtcccg atccggcaag tgatttttat
     6001 catcaatatg caaaggacat tgaattgtgt gaacgttttg gtgtaaatgg tctaagatta
     6061 tccattgctt ggagccgcat ttttcctgat ggagctggga aaccaaatcc agaaggaatc
     6121 gctttttatc atcgtgtgtt cgaagaatgt aagaaaagaa acgtaacgcc gtttgtgacg
     6181 ttgcatcatt ttgatacccc taaacgtttg tttgaccatg gagatttttt gaatcgagaa
     6241 acaatagaag catttgtttc ctatgccatt ttttgttttc atgaattcaa agaagtcaag
     6301 gtgtggagta cctttaatga aatctatcca gttgcaacca atcaatattt gttaggtgtt
     6361 ttcccaccag gaatcaaata tgattttaca aagatcgtcg cctgtcttca taatatgatg
     6421 gttgctcatg cccgtgtagt gaactatttc aaagaaaacg aacttccggg tgaaattggt
     6481 gtagttcact cattagagac gaaatatgcg gcaacagacg cgccggaaga taagcatgct
     6541 gcctttttag acgatgcatt gtcgatccgt ttcttattag atgctaccta tttaggctat
     6601 tattcaactg aaaccttgac tgctcttgat gagatatgtg aagcaaatca ggccagctat
     6661 cattttcctg aagaagattt cgttgaattg aaaaaagcat ccacgcgtaa tgactactta
     6721 gggatcaatc actaccaatg ccattttgtc aaagcttacg atggagaaaa tgcaatccat
     6781 cataacggta cgggtgaaaa agggacctct gtttataaag tgaagggaat cggagaacgt
     6841 atttataaag aaggaattcc tcgaactgat tgggactggc tgatttatcc agaaggtcta
     6901 tatgacttgt tgctgcgaat taaaagcgat tacccacatt acaacaaaat ctatatcact
     6961 gagaacggga tggggtataa agatcagttt gaagatggca ttattatgga tcagccgaga
     7021 atcgactatt tgagagtcta tttggaatcc ttaagcaagg cgataacagc cggggtaaat
     7081 gtgaaggggt attttttgtg gtcgcttatg gatcttttct catggacaaa tgggtataac
     7141 aaaaggtatg gcttgtttta tgttgatttt gaaacccaga agcgttaccc aaaagaatca
     7201 gcttactggt acaagttggt cagtgaaacc aaaacgatta tttaataagt gataatggag
     7261 gaagaaaaga tgaataaaga ggaattacaa atgcttggtt ttgagatcgt tgcatattca
     7321 ggcgacgctc gcagtacatt attgaaatta ttgaaagaag taagacaagg aaactttgaa
     7381 catgttgatt cggcgttgaa agaagccgac gaaaatttaa cccttgcaca taattcgcaa
     7441 acaaaaattt tagcggaaga agcatctggg aaagagatgg agatgggatt catttttatc
     7501 catgggcagg atcatttaat gaccacttta ttgctgagag atctgattca agactttatt
     7561 gtactgtaca gacagaatca aggataggag aagaacgcta tggatggaat cattaaacaa
     7621 atcgaaaaag ggaaaccatt ttttgaaaaa atctcacgta atagttattt aggagccgtt
     7681 cgtgacggat ttttaacagc gatgccagct attttatttt caagcatttt cattttgatt
     7741 gctgcggttc ctgagatttt tggttaccaa tggcctgaat ctgtctccac atggctatgg
     7801 aaagtctata attattcgat gggtgttgta gggttactcg tttcagcaac cactgctcgc
     7861 tgcttggctg attcgatgaa tcgtcgctta ccgaaaaaca aagtaatcaa caccacctct
     7921 gtgatgctgg cctctgtggt aagtttcctg ttgttaagcg tctctccgat tgaaggtgga
     7981 ttttcaacgg aatatatggg gacaaaggga ctactggctt cctttgtcgc tgcattttta
     8041 acagttaatt tatataagtt ttgtgtgctg aaagatatta ccattaaaat gcctaaagaa
     8101 gttcctggaa cgatttcgca aaccttccga gatatttttc ctttctcttt ttcagtattt
     8161 gcggccgttt taattgattt aggcattcga caaatcttcc aaatctcatt tgcagaagca
     8221 atcattacgg tgctgcagcc gctatttaca gcagctgacg ggtacttagg aatcgctatt
     8281 atctggggag cgatggcgct gttttggttt gtcggtgttc atggaccgtc gatcgtggag
     8341 ccggcaattg cggcaattat ctatgccaat gtggaaacca atctgcaatt gttcaattcc
     8401 ggtgaacaag cttctaaagt actgacggtg ggtttaggga attttgtagg aactatggga
     8461 ggaactgggg caacattggt tgttcctttt atcttcctac tctttgctaa gtctaagcag
     8521 ttgaaggcag ttgggaaagc ttcctttatt ccagtaagtt tcgctgtcaa cgaaccattg
     8581 ctatttgcgg cacccatgat tttgaaccct tattttttta ttccattttt gttaacacct
     8641 gttgtaaacg tttgcctgtt caagtttttt gtcgatgtat tgggcatgaa cagctttatg
     8701 tacgttttgc cttgggcaac tccagcaccg atcggtttgg ttctaggaac gggcatgggt
     8761 atcttagctt ttgttctagc agcggttcta atcgtcgttg acttcctgat ctatctacca
     8821 ttttgcaaag catacgatgc tgtcttagta gagaaagaag cacaaaaagc agaggtgatc
     8881 gcaaccgagg aaggtgcttc tgccgatgta cgtgaggaag tacctgtggc agcgagtcaa
     8941 gttatcagcg atgcaaataa agaaatcaat gtgttggtac tttgtgctgg cgcagggaca
     9001 agtgcgatgt tagctaatgc tttaaaaaaa ggtgcatctg aatcgaaagc accgatcagt
     9061 gcaatggcgg gtgcttatgg ttctcattac gaaatgctgc aggattttga tatggtggtc
     9121 ttagcaccgc aagtcaattc ttactatgat gatatcaaag aagacaccga tcgcttagga
     9181 atcaagctgg ttgcgacaaa aggagcagaa tatatcggat taacgagaga tcctgaaaaa
     9241 gctgttcagt ttattctgaa ccaatttcaa taaaggagga gccataatga aaatgggaat
     9301 ttggttgcgt aaagctgttt tatttgcagc tgtgttaggg gctttggggg ctgtttcaaa
     9361 aactgttgaa gcatctgcca gcgaatttat tcgcggagca gatatttcga tcctgtcaga
     9421 tatggaaaaa tctggtgcaa aatattatga agacgatatt tcaaaagatg ccttagagat
     9481 tttaaaaaat aatggtgtca actatgttcg actgcgtttg tggcaggatc cttataatag
     9541 taatggagaa agttatggag cagggacaaa tgatctggaa acgacgattg accttgcaaa
     9601 acgagcaaaa aatctgggac ttaaggtgct gctggatttt cactacagtg atttttgggt
     9661 tgatcctggg aaacagaatc ttcctaaagc atggcaaggt ctgacctttg aagaaatgaa
     9721 tacggcattg tacgactata caaagaacgt tctgtcggaa atgaagcagc tggacgttta
     9781 tcctgatatg gttcagatcg gcaatgaact gaatagcgga atgctctggc cttatggcaa
     9841 gtcttgggga gaaggcgggg gagaatttga tcgattggct gcttttttga agtctggtat
     9901 ccaagctgtt cgagacactc aacctaaaac aactccagta atgctccact tagctgatgg
     9961 tggcgataca ggagcattta cttggtggtt tgatgaaatc acgagtcgag gtgtgtcatt
    10021 tgatttaatt ggagtgtcgt attatccgta ttggcacggt tcaatgaatg aacttcaaac
    10081 caatatgaat gcgatcagcc aacggtatgg taaagaagtg atcgtagttg aaacagctta
    10141 tgggcataca acagcgaatg ccgatactat gccaaatgct tttggtgaag ccgaagcagc
    10201 tgcgggggga tatgaaccaa caccgacagg acaagcagag tatttgctgg atcttgccga
    10261 tcggattcag gcagtaccga ataatcgcgg ggcgggcttc ttctattggg aacctctttg
    10321 gtacaacgga aatgtgagtt gggcaacaca agcaggtatg aattatctca gtgttcaatc
    10381 cactatggga aacgaatggg acaaccaagc gatgtttgat tttgctggaa acgccttgcc
    10441 aagcttgcgt gcgtttaaac aagcaggggc gcaaataaat ctagttaaaa atgcaagttt
    10501 cgaaagcgat ggaccaacga ccagtccttc caattggcaa acctggtttc aacaaggaac
    10561 cgctaagggt acgatcaaaa cggaagcggg cacttttttt ggggactaca aactaacttt
    10621 ttggcaggat caagcatacg aggcatcagc ttatcaaaat gttagtggca taagcaaagg
    10681 aacttacaca ttgagtgcct atgtaatgtc tgggggagag tttgatacta atcagttata
    10741 tatcaaaaat tatggtggag cggaactaaa tactgcggtt gttaaaacaa gtgaatggac
    10801 aaaaattaca atcaaagaca ttccaattac aacaggtgtt tgtgaaattg gggtttatag
    10861 caaggcttca ggaaatgctt ggacgaacgt tgattttgtt tcattggtga aacaatagac
    10921 tgcggatcgt ttcgattcaa gatcggaggt tcccttcagc cacatcgtta gcgtagaagg
    10981 caggaatcga acctgcacct cgcctttgcc aaaaataacc agaaacgatt tccagtcatt
    11041 tttaccaaaa cgagtaaagc accagacttc atctacactt tgtattatgc taaagagagt
    11101 tcaacgattt cgtaagaagc aatgaaaaaa ttgcaaaaag ttgtgcagat aacataaagt
    11161 ttgtttaaga aagggtaacc ttagaattcc gtcgtgaata tgatttgagc acttagatgg
    11221 atagaaaaaa gttctcggaa aacagttcgt ttttcgagaa cttcttttta tgataagaat
    11281 gaaacaaatt aacttatatt atagtctctt ttggtaggga accatcttat ttttaatgaa
    11341 aatcaagcat tttagctatt gaagaatgtt gatttatagg gattccccct ccatattatg
    11401 ttggctcttt attcccactc cactgttgct ggtggtttag aagtgatgtc atacacgata
    11461 cggttgacgt gggctacttc gtttacgata cgtacagaga ttttttgcaa gacttcccat
    11521 gggatacggg caaagtcagc ggtcattccg tcgattgaag ttacagcacg gataccgact
    11581 gtgtagtcgt aagtacgtcc gtcacccata acaccgacag agcggatccc tggaagaaca
    11641 gtgaaatatt gccagatatc acggtcaagt ccagcagctg cgatttcttc acgcaagata
    11701 gcatcagatt cgcgtacgat ttctagcttg tcttcagtga tttcgccaag aacacgaata
    11761 ccaagaccag gaccagggaa tggctgacgc caaacgattg aatcaggcat gcctaactga
    11821 gtccctaatt cacggacttc gtctttgaat aatgtgttta atggttcgat caattcaaat
    11881 tgcatatctt ctggcagtcc acctacatta tggtgagatt tgatcgtctg tgctgtttct
    11941 gtaccagatt cgataacgtc agtatacaac gttccttgtg ctaggaaaga gacgccttct
    12001 tcaccagcaa gttttgttgc ttcgtcatca aaaacatata cgaattcatt cccaatgatt
    12061 ttacgttttt gttctgggtc agatacacct ttcaacttgt ctaagaagcg atctcttgca
    12121 ttgactttga tgatgttcaa gccgaatttt ccgcctagac tttccattac ttgttccgct
    12181 tcgcctttgc gtagcagacc gtggtcaaca aagatagatg tcaattggtc accgatcgct
    12241 ttttgtaaaa gaaccccaac gacactagaa tcaacgccac ctgaaagacc aagcaagact
    12301 tttttgtcac caacttgttc acggattttt tctacttgca tatcgataaa gttatccatt
    12361 gtccagttac cttcacagtg acaaacatca aaggcaaagt ggcgcagcaa ttcgattccg
    12421 tattcggaat gacgtacttc tgcatggaat tggataccat aaaaattacg tgttgtgtct
    12481 tggatagaag caatcggaca atctttgctt gttccgactg tttcaaagcc ttcaggaact
    12541 ttagttacca agtcaccatg gctcatccat actgtttgtt gttttggtgt ttctttaaac
    12601 aagacagctt gatcgcttgt tacttctaac aaggctttcc catattcgcg gtttttagca
    12661 ggctctacgg aaccgccaag tttgtacgtg atcaattgca taccatagca aatccctaat
    12721 acaggaatac ctaaattgaa gatctcagga tcgatcccaa atgcattctc atcatataca
    12781 ctgtttggtc cgccagaaag aatgattccc tttggattca tcgcttttac ttcttctgca
    12841 gtaatgcggt ggctcaataa ttcggaaaag actccaagat cacgaatacg tcttgtgatc
    12901 aattggttat actgactacc gtagtctaat acgataatct tttcaacgtt cgtcatatcg
    12961 gcaacgtttg tcaaatttat tacccctatc cctttaaatt ttatctcaaa atagtcaaac
    13021 tattttaaaa tgctaatatt ttcgcatgaa aatcgaatcg atgatatgat tttcagtttt
    13081 atggagaatt atatcggctc ttcctcgtgt aggcaagata tattcctgta aatttttcaa
    13141 attcacattt ttccatacat cttttgccat tttaaaggct tcttcacgat ctccgatagc
    13201 aaaacgataa taataattct ctggattttg gaaagccgta tccagtaacg caccaaaacg
    13261 atccaaatac catttttcga tcagcacggg atcggcatcg acatagacag aaaaatcaaa
    13321 aaagtcgctg acgtagattt gctgattcgc aggtaattga agcgtattga tcccctcaac
    13381 gatcaagata tcaggctgct ggatcaattc gtacttgtct ggaatgatat catacacatc
    13441 atgtgagtaa acaggtactt ttatctcttc ctgaccagat tttacacggt tcaagaaatc
    13501 gatcagcatc tccatatcat aactttcagg aaatccttta cggtccatga ttccacgctc
    13561 ttttaatata cgatttggat aaagaaatcc gtcagtcgtg atcagctgaa cgtttcttcg
    13621 tttgaaggta cgagacaaaa tcgtctgcag cagtctggct gttgtacttt ttccaacggc
    13681 tacacttccg gcgatcccga taataaaagg cggtacttct acgtatttat gaagaaataa
    13741 acctttactc aagaacaaag attcaaattc tttcatataa agataaatca gatgagcaag
    13801 aggtacatag atatctcgaa catcctgcat ggaaatgcgg tcattcaaac ttttgatatt
    13861 atctagttca tcttgtgtca aaggtgcttg cccattacga taaaattctc gccattcttc
    13921 tcttgccaca cggtagtagt tcattggatc attcatatta gcctcctgcg ttctcccatc
    13981 aatggaacta ttttaacaca aaataaacgc aaaccccacc acttaagaga gattgccgat
    14041 ctttttgtat aatctttata atagaaagaa acggagtctt attcttgact tgcgcacgga
    14101 tacataaaag gatagactgt tgtcatgaaa gaaatgagaa ggatggaaga catgacaaat
    14161 cattactatt ctgaacgccc agatacggcg cacgattttg aacaatgggc ttttgagtta
    14221 aaaggcagaa cctttcaatt tgtaacagat tctggtgtat tttcacgtga aacagtggat
    14281 tatggttcac gtgtcctgat tgatgctttc gattgggaag agttacctga tgaaggaaag
    14341 atcttagatg tgggatgcgg gtatggaccg attgggttag ctttggcatt tgcgacacag
    14401 cggtttgttg aaatggtcga cattaatgaa cgagcggtaa gtctagcgca aggaaacgca
    14461 aagcgtaacc aaatcgaaca tgttgatatc catcaatcga atatctatga agcagttcat
    14521 gaagaaacct atgcagctat cgtaagcaat ccgccgatcc gtgctggaaa aaaagttgta
    14581 catgaaattt tgacaggcgc atatcctcgg ttgaagaagg gcggtacact gacaattgtg
    14641 atccagaaga aacaaggagc gccgagtgcg caaaagaaaa tggaagaaac atttggtaat
    14701 gcagaaatcg tcacaaaaga taaaggatat tatatcatca aaagtgtgaa agaataacta
    14761 gctgaaaaat agtggagcga cgaaagagtg gaactgggcg tgcatgtacg ggtacgtatc
    14821 agttcttttt ttgttgagaa gtttttaaaa acaaagaact attttttctt caaaaagaaa
    14881 tagtaggaac agagtcgttg ttttttacga taaaataaat aagatacaga acttgtatag
    14941 aaggctgcca agtcggtaga taaaggaggt ttttatcaaa aaacatactt tccttttttc
    15001 tctattcttt tcttgggggt tttggtacac tgatagggaa tttagataga agggagccaa
    15061 taggatgaag ctgaccatca gagatatcgc agaaatggca ggcgtatcag taacaactgt
    15121 ctcacagatt ttgaataaca aaggaagcag atttagtgat aagacaagaa aaaaagtgtt
    15181 gtcgatcgtg aatgaacatc attataagcc cgattatttc gcttcaaatg tgatcaatcg
    15241 acattcaaag accatcggaa tgatcgtccc tgatgtcact gatttcttct tctccaaact
    15301 gatcgaaggt gtagagagct atttgaatcc tttgggctat gtgatcatgt tatgcaattc
    15361 tcgtcatagc caagaaaatg agatcaagta tttacaagag ctttcccacc gctcagtgga
    15421 cggaatcttg ctggccacac caaatatttt acctgagaaa tatgcattag acagcggttt
    15481 ttatcaaaaa atgccaatca ttttaatcga tcgcggattg aatcgtcggg ataacggacg
    15541 gcttatcgta aaggaatatg agggcgccta tcaagctgtt tcgctgttta ttgaaaatgg
    15601 ccatacgaag atcggcatgc tgaaggaaac cactggatac tatcaattag aagaacgttt
    15661 caacggatat cgtcatgcgc tgaaagatca tggcctgcct tttaatggga aatttgttga
    15721 acatggagag ttgactgtcc aaggtggata tactgcatca aaaaaattac tcaaacataa
    15781 agatatcacg gctatttttt gcgggaatga tgccatggcg atcggatgct atcaggcgat
    15841 cgatgaattg ggcaaaaaaa tcccagaaga tatctcagtc ataggattcg atggattaaa
    15901 gctgtcagaa tacatgattc cgcaattgac gacagtgcag cagccaagtt ttgatattgg
    15961 attttatgct gcacgtttcc tgatcgatac gatcgaattt cctcaaagaa aagttccgaa
    16021 caaagtgttc gaaacaaaat taattattcg tgagagcgta aaagtattga caaagggcta
    16081 actcggcggt atattgctta tgtaagcatt tacaaaggct agttagtgct ttttttattt
    16141 ttagtaaacc agtttactaa agtggtttac ctttcctaaa aataatgtat aataccgaat
    16201 gaagacaagg aggatcaggc aatgcgaatg gtggatctga ttgaaaaaaa gagggatgga
    16261 aacgagctgt cgaaagagga aatcgagtac atcgtcacga actatacgaa tggaaagatt
    16321 ccagattatc aagtcagtgc attattgatg gcaatttttt atcaagacat gacaaatgaa
    16381 gaaatcacga acttaacatt ggcgattgct aattctggtg atgtcatcga tttgagttct
    16441 cttgaaggaa taaaggtaga taagcattcg acaggtggcg ttggtgacac gaccacattg
    16501 attttagccc ccctagtagc tagtgtcgga gtgacagtgg ctaaaatgtc tggacgcggt
    16561 ctaggttaca caggtggtac actagacaag ctagaagcaa ttcccggatt tcagatcgag
    16621 ctttcggatg aagaatttgt acgcattgtt aatgaaagta aagtagcggt aattggtcaa
    16681 tcaggaaact tggcaccggc ggacaaaaaa ttgtatgcct tgcgtgacgt cacagcgacc
    16741 gttgattctc ttccgctgat tgccagttcg attatgagta aaaaaatcgc ggcaggagca
    16801 gatgcaatcg ttttggatgt gaccactgga gacggtgctt tcatgaagaa catcgaagat
    16861 gcaagacgtc tggcaaagac catgacgagc attgggaaac tggctaaccg agaaacagtg
    16921 gcggttatct cagatatgtc agaacctctt ggcgaagcta tcggaaatag tctagaagtt
    16981 gtcgaggcaa tcgaaacatt acaaggaaat ggtccagaag atctagtaga aatgtgctac
    17041 gccttaggaa gccaaatggt tgttcttgct ggcaaagcta agacaaccga tgaagcaaga
    17101 acgttgcttc aagaagctct ggaatcaggt aaagcgttag caaaattcaa agaaatgatc
    17161 caaaatcaag ggggagaccc agcgatcgtg gaacatccag aacgaatttt gactgcacgc
    17221 tacacaatgg agcttcctgc taaacaaagc ggagttgtgt caaaaatcgt ggcaaatgaa
    17281 ttaggaattg ctgcaatgat gctaggtgct gggcgtaaaa ccaaagaaga agatattgat
    17341 cacgctgttg gattgaaact gcataaaaaa attggtgaca ctgtgacaaa aggtgagtca
    17401 ctattgacga tctatagtaa cgacgaagaa atcacttctg tcattgaatt attatataaa
    17461 aatattgaaa ttggtgaatc ggcaatgaag ccaacgttga ttcatgacat catcacagaa
    17521 taaaggaaaa aagcggactt taccgtttta aagaaaaggg gaaaagaaat ggaactaaac
    17581 cgaatgatcg accatactat tttgaaagca gacgcatcta aagaagctgt gatgcaaatt
    17641 atcgaagaag caaaaaaata ccatttttat tctgtctgca tcaatccagc atgggttgcg
    17701 ctagcgaaag aacaattaca aggtgagcca gtagccgtat gtacagtaat cgggtttcct
    17761 ctaggagcaa acacacccga gacaaaagca tttgaaacaa cagatgccat caataacggt
    17821 gcagatgaag tcgatatggt catcaatatc ggtgctttga aatcaaagga tgatcaacaa
    17881 gtccaaaaag atatcgaagc agttgttgaa gcagcaaaag atcgtgcgct tgtcaaagtt
    17941 attgttgaaa catcgttatt agatagagaa gaaatcatcc gagcttgcga aatagcaaaa
    18001 gctgcaggcg ctgattttgt caaaacatca actggtttct caacaggcgg agcaaaggta
    18061 gaggatgtcc gtttgatgcg tgaaactgta ggaccagaaa tgggtgtcaa agcttccggc
    18121 ggtatccata acgaagaaga agcaatggca atgatcgaag ctggtgctac aagaatcgga
    18181 acaagcgcag gagtagcaat cgtatctggt tcaactggtg aaggctatta attttttcaa
    18241 aggagaagct catggcagaa ctcaaacaag aatggatcaa cacagctatt gaagcgttag
    18301 ataaagccta tgttccttat tcccattttc cggtaggcgc ttgtctcgta acggaatcag
    18361 gcaagattta tcaaggcatc aacattgaga atgcatcatt tggattaacg aattgtgccg
    18421 agcgcacagc ttttttcaaa gcagtatctg aaggagaaaa aacatttacg catctagtcg
    18481 tagcggggca cacccccgat cctatctctc cttgtggtgc atgtcgacaa gtaatggcag
    18541 aattctgttc ccctgatatg ccggttacgc tagtaggaga caatggtgtg acgaaggcaa
    18601 cgaccgttcg tgcactgctg ccttatgcat ttactgaaaa agatctataa ctcatgttca
    18661 ttcagtcagc tgactgttga aaaataaaac aattatttta ggaggcttta cagaaatgaa
    18721 aaaagcaaaa ttatttggtt taggtgctgt cgcactagca gcagggttat ttttaggtgc
    18781 atgcggcaac aacggctcta ctgattcatc cggtggcaaa agctcatcag acacaacaac
    18841 tgcagcattg atcacagaca caggtggtgt agacgaccgt tcattcaacc agtcagcttg
    18901 ggaaggacta gaaaaatggg ggaaagatca aggactttca cgaggaaatg atggcttcca
    18961 atatttccaa tcttcaaatg aatcagatta catcccaaat attgaccaag cattgaatgc
    19021 tggattcaaa acgatttttg gtattggtta taaactaaaa ccagcgatcc aagaacaagc
    19081 aacaaacaat acaggtacga actttgttat tatcgatgat gtcatcgatg gattagataa
    19141 cgttgtatct gcaactttca aagacaacga agcttcttat cttgcaggtg ttgcagcagc
    19201 ttatacgact gaaacaaacg tagtcggctt tatcggcggt gtaaaaggtg aagtaatcga
    19261 tcgttttgat gctggattca aagccggtgt tgatgcaggt gcgaaagaat taggaaaaga
    19321 aatcaaagta ttgaatcaat atgctggtga cttctctgct ccagataaag gacgttcaat
    19381 tgctcaaggt atgtatgcac aaaacgcaga tatcatcttc catgcttcag gcggtacagg
    19441 taacggtgta ttccaagaag caaaatcatt gaacgaatca ggcgacaaaa aagtttgggt
    19501 tatcggtgtt gaccgcgacc aatcagacga aggcgaatac acattaaacg gcgaaaagaa
    19561 aaacttcaca ttgacatcta cactgaaagc agttggaaca gtagtagaag atttggcaca
    19621 aaaatcagcg gacggtaaat tccctggcgg cgaacacact gtttatggat tgaaagaaga
    19681 cggtgttggt ctaacagaag gacaactttc tgacgaagcg aaaaaagcag ttgatgaagc
    19741 aaaagaaaaa atcatttcag gtgacgtaaa agttccagaa actcctgaag aaaactaaaa
    19801 ccatttctat agccttgtta gacacatgtt tgacaaggct atggtttgat tagaacaaga
    19861 attgttcatt tatccaagct gccttgtgac agtttggata agggaacaac tccctgaagt
    19921 aatgtttgtt tcaaaagaag aacgctcact aagcgtgaat cttagtcaat cgttgcccta
    19981 gatagaaatc gttaggatgt gaaaaaaagg tggttcaaga aaatattgtc atagaaatgc
    20041 agaatattac caaagaattt ggaacattca aggcaaatga caacatcaat ttacaagtaa
    20101 aggcaggaga aattcatgcg ttgcttggag aaaacggtgc tggcaaatct acattgatga
    20161 acgtgctttc cggattatta gagccgacat cagggaaaat tttgatgcgt gggaaagaag
    20221 taaagatcac aagtccgacc aaagccaacc aattagggat tgggatggtc catcagcact
    20281 ttatgcttgt tgatgccttt actgtaacag aaaacatcgt gttgggaagc gagcctagtc
    20341 gcgcagggat gcttgaccat aaaaaagcac gaaaagagat ccaaaaagtt tctgaacagt
    20401 atggattgtc agtcaaccca gatgcttacg ttcgtgatat ttcagttgga atggaacaac
    20461 gggtagaaat tttaaaaaca ctttaccgag gagcagatgt actgattttt gatgagccga
    20521 cagctgtatt gacccctcaa gaaattgatg aattaatcgt gatcatgaag gaattagtca
    20581 aagaaggcaa gtcgatcatt ttgattacgc ataagttaga tgaaatcaaa gcagtagctg
    20641 accgctgtac agttatccgc cgtggaaaag gaatcggtac agtcaacgtt aaagacgtaa
    20701 cctcacaaca attagccgat atgatggttg gaagaacggt ttcattcaaa acgatgaaaa
    20761 aagaagcgaa gcctcaagaa gtcgttttgt ctattgaaaa tctagtggtg aaagaaaatc
    20821 gtggattaga agccgtgaaa aacttgaact tagaggttcg tgctggcgaa gtacttggta
    20881 tcgctggaat cgatggaaac gggcagtcgg agttgatcca agctttgact ggtttgcgaa
    20941 aggcagaaag cggacatatc aagctaaaag gggaagacat cactaataaa aaaccccgaa
    21001 aaatcactga acatggtgta ggacacgtgc cagaagaccg tcataaatac gggttagtcc
    21061 tagatatgac attatctgaa aatatcgcac tacaaaccta tcatcaaaaa ccttacagta
    21121 aaaacggcat gctgaattat tcagtaatga atgaacatgc cagagaattg atcgaagaat
    21181 atgatgttcg aacaacgaat gaacttgttc ccgcaaaagc tttatcaggc ggaaatcagc
    21241 aaaaagcaat tatcgctcgt gaagtcgacc gagatcctga tctgttgatt gttgcaaatc
    21301 caactcgtgg actggatgta ggtgcgatcg aatttattca taaacgtctg atcgaacaaa
    21361 gggacaaata caaagcagtg ttactgatca gtttcgaatt agaagaaatt ttaaatgttt
    21421 cggatcgtat tgctgttatc catgaaggag aaattgtcgg tatcgttgat ccgaaagaaa
    21481 catctgaaaa tgaattaggt ctattgatgg cgggctatac cttagaagaa gcacgaaaag
    21541 agttagaaaa gggggctggc gcacatgaat gatcgcagcg aaaaattccg taatttatta
    21601 gtgcctgtac tttcagttgt tttaggattg atcttaggcg cgatattgat ggctgctttt
    21661 ggttttcagc ccatccaagg ttattcttct atgctagatg cagcccttgg ttcgcaaaga
    21721 gggataggtg aaacgttaag gcaagcaacg ccgttgattt ttacagcatt aggtttttct
    21781 gtagctaact cagctgggtt tttcaatatt gggctttctg gacaagcact ttgcgggtgg
    21841 atcgtcagtg tctgggtggc acttgcgttt ccagatctgc caaaagttgt tttgcttccg
    21901 ttatgcgtga tcttaggtgc cttagctggt gcgatggcag cggctgttcc aggtttactt
    21961 cgagcatttt ttggaacaag tgaagtgatc gtaacaatca tgatgaacta tatcttatta
    22021 tacgtaagca catttgcttt acatgaagtg atgcctgaaa gctatcgctc aagtttggac
    22081 tcttccaata tgatcagtca aaatgcctca ctgcggttac catggttaac acaagctttt
    22141 ggcggctctc gcgtaaactc aggattattt ttagcaatca tcgcgcttat tttcgtttgg
    22201 ctgctgatga agaaaacgac tttaggattt gaaatccgtt ctgttggatt gaatcctttt
    22261 gcttctgagt atgcaggaat gagtagtaaa cgtacgatcg ttttgtcgat ggtcatttct
    22321 ggcgctttgg cagggcttgg cggtgtggtt gaaggattag gaacgtatca aaacttcttt
    22381 gttcaaacga cttcgctatc tattggattt gacggaatgg ctgtttcctt gcttggttct
    22441 ggatcagcaa tcggtattct tttatcagcg atcttgttca gtattttgaa gatcggtgga
    22501 cttggcatgc agacaggagc aggtgttccg tttgagatcg tcaatgtatc gattgctttg
    22561 attatcttct ttgtcgggat caacttcctg attcgtttcg tcatggctaa agtcttccaa
    22621 gggaaaaaac aagaagagat cgttgcgaca atagaatcga aaccaacgaa tcaaaaccat
    22681 gaagggggcg aactataatg tcgactatcg aattaattgc cttgatcctt acttctacac
    22741 tggtttattc aacaccgcta attctaacgt cactaggcgg aactttttct gaacgaagcg
    22801 ggatcgtaaa cgtagggcta gagggaatca tggtcatggg tgcttttagt tccatcgttt
    22861 tcaacttgac gatgagtcca tcacttggtt ctatgacacc atggatcgct attttagtgg
    22921 gtggcgtcgt aggtatccta ttttctctga ttcacgcagt agcaacagtc agtttgcgtg
    22981 ctgaccatat tatcagcggg acagtaatta atctgatggc acctgcttta ggtgttttct
    23041 tgatcaaagc atggtatggt aaaggtcaaa ctgataacat cacgcagaat ctggggtatt
    23101 tttctttccc aggattagca gatattccgg tcattggacc aattttcttt aaaaatacat
    23161 tcttacctgc ttattgtgct attttgattg ctgtcctagc ttgggtgatc atcttcaaga
    23221 cgaaattcgg cttgcgcctc cgttcagttg gtgaaaatcc tcaagctgcc gacacattag
    23281 gtatcaatgt atatgctatg cgttattcag gcgtactgat ttctggcttg ttaggcggta
    23341 tgggcggtgc tgtttttgct cagtcgattt ccggtaactt ttcagctggt acgatcgttg
    23401 gacaagggtt catctccatg gctgctatga tctttggtaa gtggaatcct ttaggggcaa
    23461 tgggtgcctc attgttcttt ggttttgctc aaagcttgag tatcattggc gcacaattac
    23521 cagttatttc ttcgattccg ccagtattgc tgcaaattgc tccatatttg ctgacaatca
    23581 ttgtgctggt tgttttctta gggaaagctt ctggtccaaa agcaaacggg aagaactata
    23641 tcaaatcaaa ataatctttt agcgaataaa aagggtgggt tacgggaagt gtttttcgtc
    23701 tcgccctttt tgttttaaag gcaataaaaa taaaaagaaa gaaggaataa tcatgtttaa
    23761 acgcgtacat ctgatcgtca tggattcagt aggaattgga gaagcaccag atgccgataa
    23821 gttcggtgat gttggttctg atacattagg acacattgcg aaagaagcag gattgaacgt
    23881 tccacattta gagcagcttg gtttaggaac gatccgtcct cttgaaggtg tggaatcagt
    23941 gaaagatcat caaggctatg cgacgaaatt agaagaagtt tctgtaggga aagatacgat
    24001 gacaggccac tgggaaatca tgggattgaa tatcaaaact ccatttcgtg tgtttcctaa
    24061 tggattccct gatgatctga tcaaacaaat cgaagaattt tctggccgta aagtcgtatg
    24121 taacaaacct tatagcggaa cagctgttat tgacgattac ggtgaacacc aaatgaaaac
    24181 aggcgattta attgtatata cttctgccga tcctgtcctt cagatcgcgg cacatgaaga
    24241 aatcattcct ttagaagaat tatatcggat ttgccaattc gttcgtgata taacaaaaga
    24301 tgatccgtat atgattggcc gaatcattgc acgtccgtac gtgggtgaac caggagactt
    24361 cacacgaaca agcaatcgcc atgattatgc gttgaatcca ttcggacaca cagtccttga
    24421 ttctttgaaa gaagcaggaa aggacgtgat tgctgttgga aagatcaacg atattttcaa
    24481 cggacaagga atcactgaat ctgtccgcac gaaaagcaat atggatggcg ttgaccagct
    24541 gctgaaagtt atgaaaaacg atttttcagg aatcagcttt accaatttag tagattttga
    24601 tgcattatac ggtcatcgtc gggatgtagt cggctatgcg catgcgattg aagaatttga
    24661 cttacgaatt ccagaattgc ttgaagcgat ggaagaagac gacttgctaa tgattacagc
    24721 cgatcacgga aacgatccga cgtttactgg taccgatcat acgcgagaat atgtaccgtt
    24781 attggtttac agtaaaaaaa tgaaagaaca aggggcattg cctcaaggat attattcaga
    24841 tatttcagca acgattgctg agaactttag tgtttcttcg actgaaaatg gcgaaagctt
    24901 cttgaaatta ttaaaataat ctttttaaat aggagaaagc aaatgaccaa gttaagcgag
    24961 atgttacaag aaacgaccca gtttatcctt gataaaggag taaaagaaat tgattttggt
    25021 ttgattctag gttcaggatt aggtgaatta gcagatgaaa tcgaagatgc tgtaatgatt
    25081 tcttatgatg agatcccgtt ctttcctgtt tcgacagtag ttgggcatgc aggaaagcta
    25141 gtttatgggt ctctggcagg caagaaagtg ttagccatgc aagggagatt ccactactat
    25201 gaaggtcatt ccatgcaaac agtgacctat ccagtacgtg tgatggccgg actaggggcc
    25261 cattcagtca ttgtgactaa tgcatgtggt ggtgtcaacg aatcatttgt accaggagat
    25321 ttgatgctga ttaccgatca tatcaatttt actggtgaaa atcctttgat cggtccgaat
    25381 gaagatgcaa tgggccctag atttccagat atgagtcaag cttatacgcc tgactatcaa
    25441 gcaacagcaa aagcagcagc agaaaaacta ggtttggatc ttaaagaagg cgtatatatg
    25501 ggctattctg gaccaactta tgaaacgcct gcggagatcc gaatgtctcg gacaatagga
    25561 gccgatgcag tagggatgtc gactgttcca gaagtcatcg tagcagctca cagcggatta
    25621 aaagtgttag gaatctcttg tattaccaac ttagcagcag gtatgcaagc gaatctgagc
    25681 catgaagaag ttgtagaaac aactcagaga gtcaaacaat cttttaaagc attggttaaa
    25741 gaaacattga cattattgta ataggaggaa caataaaatg agtgtgcata ttgaagcgaa
    25801 agaaggcgag atcgccgata agattttact tccgggagat cctttgcgag ctaaatatat
    25861 tgcggagact tttttggaag atccagtttg ttacaatcag gtacgcggta tgttaggctt
    25921 taccggcaaa tataaaggag aacgtatttc tgttcaagga acaggaatgg gaatgccatc
    25981 agccaccatc tatgcccatg aattgatcca gtcgtatggt gtaaagaaat tgattcgagt
    26041 aggtacatgc ggtgcattgt caaaagacgt ccatgttcgt gatttagtac tagcacaagg
    26101 tgcagcgacc agttcttcga tgatcgaaaa aaatttccaa gcctttcatt ttcctccaat
    26161 cagtgatttc aatctattac tgaaagcata tgagatagct aaagaaaagg gctatacggt
    26221 tcatgtagga aatgttcttt cggaagattc tttctacaaa gatgatttga cagagacgtt
    26281 ccaattagct gaacttggcg ttttaggagt ggaaatggaa gcagcggctc tttattactt
    26341 aggcgcgaaa taccatgtgc agacattaag tttgatgacc gttagcgacc atttgatcac
    26401 cggtgaagaa acaacagctg cagaacgtca atctacgttc aatgaaatga tcgaagtagg
    26461 attggaaaca gcgatagcag aataagaagg aaatgaaaaa gctgccctgc attaagtaat
    26521 aagaacatcc aagcaaaaat agctcctcat gtttttgact tggctggact tattattgca
    26581 aatgtaagct tttgaacacc gtttaccaag aggttgtgaa tctgccgttt aactctgagt
    26641 cacaaggaac aatttcaaaa aacagctttt catgtttgat gaatattgtg acttgtgacc
    26701 gagaagttgg caggagaaaa ccgtttatac gaaaaaaggg actgtgacaa atcgtgtcac
    26761 agtccctttt ttatagtgtt ttttataggt agtatttttt agcaactgtt aagtcatcat
    26821 ttaattcata aacaagtggt tgacctgttg gaatttcaag atccatgatg tcttcgtcag
    26881 agataccttc gatatgttta gctagggcgc gtaatgagtt accatgtgca gctactaaaa
    26941 cagttttgtt gtctagtaat gctggagcaa tttcatcttg ccagaatggc aaagcgcgtt
    27001 ctaatgtcac tttcaagttt tctccaccag gaatatcgcg tttgtctaac attgcgtaac
    27061 gacggtcatt tgctgcagaa ccttcgtcag tttcttccat taatggagga agagtatcgt
    27121 aagaacgacg ccagatgtgc acttgttcgt cgccgtattt ttcagctgtt tcttttttgt
    27181 tcaaaccttg taatttacca tagtgacgtt cgtttaagcg ccatgatttg atttgtggta
    27241 cccaaagttg atctgaatat tctaagatca agttacatgt tttgattgca cgagttaaaa
    27301 cagaagtgta agcaacatca aattcgattc cagcttcttt gattttgcgt ccgccttctt
    27361 ttgcttcttc gattccttct ggtgctagat ttacatcagc ccaaccagta aattggttca
    27421 atgcattcca ttcactaaga ccgtgacgag aaaaaactaa ttttggcatg tttagctctc
    27481 cttttaagat aggtttatat ttctgaaatt ttcagttctg ttttattgta caattttttg
    27541 gaagagattt ccatcgaaaa attagataag attctatgag aaaacaagta gagaatataa
    27601 aaaaacgaac agtgcccacg caactgttcg tttttaggta ttagatgcat aattgctcga
    27661 tttctttttc aggtaaattg atatatttat aacatgttcc aactgaaaca ccgcattttg
    27721 aagaaataaa ttggatcgtt tctttttttt cataatagag acgtcggatt tttttgatcg
    27781 tttttgcatc gatttttggt cttccgccga tttttccttt tttacgagct tcatctaatc
    27841 caactaatgt acgttctttg atcaatgcgc actccatgtt tgctaggttt tccattaatt
    27901 ggaaatagat ttttcccatt tcgcgcgtat cgattccttc atcaagacta atcaagtgca
    27961 gcccgcgatc tttgaataat tgcgtgagtt ctgttaattg ccgcgtagat ttgcctaatc
    28021 gatgaagttg gcaaatgact agtgcatcgc cttgttgcat gtcagcgatc actgactcca
    28081 aatgaacagc agtagaatca ttttgttcca cttcatatgt ctcatggaaa atttcttgac
    28141 aaccaaagtc agataattga tcaacctgag ttgttaaatc aacgtcattt atggttgtgc
    28201 gtgcataacc aataattttc atggtgattc ctttctagca gtgattttct aactggatat
    28261 aacagtaaaa atgtagcata aagtacgtca aaaagatagt tttttcgcaa attttccgct
    28321 gtcttttttt attggatgat caagcagttt ttctttattt acgacggatt gcgtcaagaa
    28381 tctcaacgcc tttttctgag ccgacaacta ctttagaaac catgtttagg aaaagcccat
    28441 gttctactac cccaacaagt tcatctaaat aggtagctaa gctttctggc tgttcgatga
    28501 cttctagatg cagatcaatg atgtaatgac caccatctgt ggttaagact tctccgtttt
    28561 cgtctgtacg caagacgggt tggaatcttt tttcttcaaa aatatgaagt agctgttggg
    28621 agccataagg gatcacttca actgggagtg ggaacttccc aagtttatcg actaatttag
    28681 aagaatcaac gatccaaatc gtttcttttg agtacgtagc gactattttc tcgaaaagca
    28741 aagcagcgcc gccgcctttg atcccttgaa aatcagcact gatttcatca gcaccgtcta
    28801 ttgtcaaatc gactacgggg acttcatcaa tactctttaa cggtattcct aatttttccg
    28861 cttgttcttt tgtcgcattt gaagtagtaa ctcctgtgat acgtaaacct tcttcacgca
    28921 tacggcgacc aatctcttct accatataat aggcggttga accggtcccg agacctacga
    28981 tcatcccgtc ctttacgaat ttcgcggctt caatgccagc taattgtttt aaattcatct
    29041 ttgcgtccac ctttttttag tttgagcatc gtgctttcct cactatttta gcgtgattta
    29101 cggtaaaaag ataccattct tttttattcc aataaagctt tttcaaaaaa aattgcatct
    29161 cttgttgaca tgttttttca ttcgtgatat tctaacaaag gtgctttatc tacaggtttc
    29221 tgtgaagaaa cgtgctgact gtttatcaaa gcatatttga taaggatgca gacattgcat
    29281 ttatttttta tcacaaaaaa agaaccacct ggatgtgtgg aactagacgc aaatcgtggg
    29341 aaggaggaaa caaggaatgc ctacaattaa ccaattagta cgtaaacctc gtaaatcaaa
    29401 ggtggaaaaa tctaattcac ctgctttgaa caaaggatat aacagcttta agaaaactca
    29461 aactaacgta aactcacctc aaaaacgtgg ggtagcaact cgtgtgggta caatgacacc
    29521 taaaaaaccg aactcagctt tacgtaaata tgcacgtgtt cgtttgtcaa acttaattga
    29581 agttactgct tatatcccag ggattggtca taacttacaa gaacacagtg ttgtattatt
    29641 acgcggtggt cgtgtaaaag acttaccagg ggtacgttac catatcgttc gcggtgcttt
    29701 agatactgct ggtgttaacg accgtaaaca aagccgttct aaatacggaa ctaaaaaacc
    29761 taaagcttaa ttttaattat taaacaaatg aaacttgctt ctactatata gaagtaatta
    29821 ctatgaacaa tatttcggaa ggaggagtta cggatgccac gtaaaggtcc tgttgcaaaa
    29881 cgtgatgttt taccagatcc tatttataac tcaaaattag taactcgctt gatcaaccgt
    29941 gtaatggttg acggaaaacg cgggattgca gctaacatta tctataatgc ctttgacttg
    30001 atcaaagaat ctacaggtaa cgatccatta gaagtgtttg aacaagcaat gaaaaacgtt
    30061 caacctgttc ttgaagttaa agcacgtcgt gttgggggtt caaactacca agtaccagtt
    30121 gaagttcgcc ctgaacgccg cgttactcta ggtcttcgtt gggtagtaaa ctacgctcgc
    30181 ttgcgcggtg aacacacaat ggaagaacgc ttagcaaaag aaatcatgga tgctgcaaac
    30241 aacacaggtg cttctgttaa aaaacgtgaa gacactcata aaatggctga tgccaaccgt
    30301 gcgtttgctc actatcgctg gtaagattct ctctgtttgg tcggctttta taagtcgacc
    30361 tcagatagtg tccttatttt tagcaactag atagtaaaga gaggagaaac aaggagaatg
    30421 accagagaat tttctttaca aaacactcgt aatatcggta tcatggccca catcgatgct
    30481 ggtaaaacga ctgctactga acgtatcttg tactatactg gtaaaatcca taaaattggt
    30541 gaaactcatg aaggagcttc acaaatggac tggatggaac aagaacaaga acgtggtatt
    30601 acgatcactt ctgctgctac tacagcacaa tggaagggtc accgaatcaa catcatcgac
    30661 acaccaggac acgtagactt cacagttgaa gttgaacgtt cattgcgtgt acttgatggt
    30721 gctgtaactg ttcttgatgc acaatcaggt gttgaacctc aaactgaaac agtttggcgt
    30781 caagcaacaa cttatggtgt acctcgtatc gtatttgcaa acaaaatgga caagatcggt
    30841 gctgacttct tatattcagt atcaacttta catgaccgtt tgcaagcaaa cgctcaccca
    30901 atccaattac caattggtgc ggaagacgac ttcacaggaa ttatcgacct tgtaacaatg
    30961 aaggctgaaa tgtatacaaa cgatcttgga acagagatcg aagaaacaga aattcctgaa
    31021 gaataccgcg aattagctga agaatggcgc gaaaaattgg ttgaagctgt agctgaaact
    31081 gatgaagaac ttactttgaa atacttagaa ggtgaagaaa tcactgaagc tgagttaaaa
    31141 gaaggtatcc gccgcgcaac agttaacgta gaattctacc cagtactatg tggttcagca
    31201 tttaagaaca aaggggttca attactattg gacgctgttc ttgattactt gccatcaccg
    31261 ttagatatcc cagctatcaa agggattgac cctaaaactg atgaagaagt agaacgtcct
    31321 gcagacgaca gcgctccttt ctcagcttta gcatttaaag ttatgactga cccattcgta
    31381 ggtcgtttaa ctttcttccg tgtgtactct ggtgttttac aatcaggttc atacgtacaa
    31441 aacgcaacaa aaggcaaacg tgaacgtgtt ggtcgtatcc tacagatgca tgccaactct
    31501 cgttcagaaa tctctgaagt ttacgctgga gatatcgctg ctgctgttgg tttgaaagac
    31561 acaactactg gggacacact atgtgatgaa aagagtctag ttattcttga atccatggaa
    31621 ttcccagaac cagttatcca agtcgctatt gaacctaaat caaaagctga ccaagataaa
    31681 atgggtgtgg ctttacaaaa actatctgaa gaagatccta ctttccgtgc tgaaacaaac
    31741 gtggaaacag gcgaaacaat catcgctggt atgggtgaac ttcacttaga tatcatcgtt
    31801 gaccgtatgc gtcgtgaatt caaagttgaa gctaacgtag gtgcgcctca agtatcttac
    31861 cgtgaaactt tccgtgctgg cactcaagcc gaaggtaagt ttgtacgtca gtctggtggt
    31921 aaaggtcaat acggtcacgt atggattgaa tttacaccaa atgaagaagg tgctggtttc
    31981 gaatttgaaa atgcgatcgt cggtggggtt gttcctcgtg aatacattcc agctgttgaa
    32041 actggtttga aagatgctat ggaaaatggt gtcttagctg gctatccatt agtagatatc
    32101 aaagctaaac tttacgatgg ttcataccat gatgtcgatt caaatgaaac tgccttccgt
    32161 gttgctgctt ctatggcatt acgtgcagca gctaagaaag ctaatccagt aatccttgaa
    32221 ccaatcatgg ctgtagaagt tgttatccct gaagattatt taggtgatgt tatgggtcac
    32281 gtaactgctc gtcgtggacg tgttgaaggt atggaagctc gcgctggcgg ccaacaagtt
    32341 gttcgtgcga tggttccact tgctgaaatg tttggttatg ctacaactct tcgttctgct
    32401 actcaaggtc gtggtacatt tacaatgaca tttgaccact acgaagatgt accaaaatca
    32461 gtacaagaag aaatcattaa gaaaaacggc ggaaaagctg aataatttct taaaaccgga
    32521 tagtcctgca aagagattat ccaaagagac tatattttta atatagtttc gtgtaaaata
    32581 attatcgatg atatgggcat gtggtgtacc ttgtgtacat catataccta tcagacaaat
    32641 aaaaaatata ttgtattttt aggaggacat tttaaaatgg caaaagaaaa atttgaccgt
    32701 tctaaaccac acgttaacat tggtactatc ggacacgttg accatggtaa aactacatta
    32761 acagctgcaa tcacaactgt actatctaag aaaaacggcg gccaagctat ggcttacgat
    32821 caaatcgatg gtgctccaga agaacgcgaa cgcggaatca caatctcaac agctcacgtt
    32881 gagtatgaaa cagatacacg ccactacgct cacgttgact gcccaggaca cgcggattac
    32941 gttaaaaaca tgatcactgg tgctgctcaa atggacggag ctatcttggt agtttctgct
    33001 gctgacggcc caatgcctca aactcgtgaa cacatcctat tgtctcgtca agttggtgtt
    33061 ccttacatcg ttgtattctt gaacaaagta gacatggttg atgacgaaga attactagaa
    33121 ttagttgaaa tggaagttcg tgacctatta acagaatacg aattccctgg tgacgatgtt
    33181 cctgtagttg ctggatcagc tttgaaagct ctagaaggcg acgcttcata cgaagaaaaa
    33241 attcttgaat taatggctgc agttgacgaa tacatcccaa ctccagaacg tgacaacgac
    33301 aaaccattca tgatgccagt tgaagacgtg ttctcaatta ctggacgtgg tactgttgct
    33361 acaggtcgtg ttgaacgtgg acaagttcgc gttggtgacg aagttgaagt tgttggtatt
    33421 gctgaagaaa cttcaaaaac aacagttact ggtgttgaaa tgttccgtaa attgttagac
    33481 tacgctgaag ctggagacaa cattggtgct ttactacgtg gtgttgcacg tgaagacatc
    33541 caacgtggac aagttttagc taaaccaggt acaatcacac ctcatacaaa attctctgca
    33601 gaagtatacg tgttgacaaa agaagaaggt ggacgtcata ctccattctt cactaactac
    33661 cgtccacaat tctacttccg tacaactgac gtaacaggtg ttgttgaatt accagaagga
    33721 actgaaatgg ttatgcctgg cgacaacgta acaatggaag ttgaattaat ccacccaatc
    33781 gctatcgaaa acggtactaa attctcaatc cgtgaaggcg gacgtacagt tggtgccggt
    33841 gtcgttacag aaatcaaagc ttaattcact taagttttaa agtaaagcaa catttattcg
    33901 gacgtaatcg ctcggaacga aggtcttagg atctttgttc cgagcttttt tatttattgc
    33961 aggatcagga aaaaagacag gagtattata aatagaagaa acacgaaata aaagagtcag
    34021 tattttctaa atgaaacttt atgaaaacaa aatgtttttt acaagaaaaa gccttgcttt
    34081 gtgagtttat atatattata atgacaaagg tgcgtttact ttagtgaatt gcacagacgt
    34141 aatcagaatg atttcgtcgg ctaagaaacg agaggttgcg acacgcccgg acgctttgcc
    34201 acgaacgagc gtgacggaaa ttttcgtgga gctatgtcta cttttcaaaa taggcgaagg
    34261 agggaacaag atggcaaaac aaaaaattcg tatccgttta aaagcgtatg aacaccgtat
    34321 tttagatcaa tcagcggata aaatcgtgga aactgcaaaa agaactggag ctagcgtgtc
    34381 tggtccaatt ccattaccaa ctgaacgcag tctttacaca atcatccgtg cgactcataa
    34441 atacaaagat tcacgcgaac aattcgaaat gcgtacacac aaacgtctta tcgacattgt
    34501 gaacccaaca ccaaaaactg ttgatgcttt gatgaagcta gacttaccat ctggtgtaaa
    34561 catcgaaatc aaattataaa actaaaacaa aaataatgga ggtgtactca tgaccaaagg
    34621 aatcttaggg aaaaaagtgg gaatgacaca aatcttcact gaatctggtg aattaattcc
    34681 agtaactgtt gtagaagcta ctccaaacgt agtactacaa gtgaaaacaa tggaaacgga
    34741 tggctacgaa gctattcaag ttggttacca agatatgcgt gaagttttat caaacaaacc
    34801 tgcgaaaggt catgttgcaa aagcaaacac ggctcctaag cgcttcattc gtgaattcaa
    34861 aaatgttgag ctaggagaat atgaagtagg aaaagaaatt acagtagatg ttttccaagc
    34921 aggagacatt attgatgtaa caggtactac gaaaggtaaa ggattccaag gggttatcaa
    34981 acgccacgga caaagccgcg gaccaatggc tcacggttct cgttaccatc gtcgtcctgg
    35041 gtcaatgggt ccagttgcac ctaaccgtgt atttaaaaac aaacgtcttg ctggccgtat
    35101 gggtggcaac cgcgtaacta tccaaaactt ggaagttgta cgtgtcgatg cagaaagaaa
    35161 tgttatcttg atcaaaggta atgttcctgg agctaaaaaa tctttaatta ctatcaaatc
    35221 agctgtgaaa gctaaataat tgtaggaaga aaggaggaac taaggaatgc cgaatgtagc
    35281 attattcaaa caagatggaa gccaaaacgg tgaaatcaca cttaacgaag aaatcttcgg
    35341 aattgaacct aatgaaagtg ttgtttacga tgcaatcgta atgcaacgtg cgtcattaag
    35401 acaaggaaca cacgcggtta aaaaccgtag cgctgttcgc ggcggcggac gtaaaccatg
    35461 gcgtcaaaaa ggaacaggtc gtgcacgtca aggatctatc cgctcaccac aatggcgtgg
    35521 aggtggcgta gtctttggac caacaccacg ttcttacagc tacaaacttc ctaaaaaagt
    35581 tcgtcgttta gcaatgaaat ctgtattgtc agaaaaagtt gctgaaaaca acttggtagc
    35641 aatcgaaggc ttgaacttcg acgcaccaaa aacaaaagaa ttcaaacaag ttcttgctaa
    35701 cttatctatc gactctaaag tattggttgt tctagaaaaa ggtaacgact ttgctgcttt
    35761 atcagcacgt aacttaccaa acgtttctgt agtaacttct gataatgtga gtgtgctaga
    35821 cgttgtatca aatacaaaag tgttagcaac acaaactgct cttactcaaa ttgaggaggt
    35881 gcttgcataa tgaacttact agacgtaatc aaacgcccag tgatcactga aaaatcaatg
    35941 cttgccatgg atgacaagaa atacactttt gaagtggaca ctcgcgcaaa caaaacttta
    36001 gtaaaacaag cggtagaagc tgcttttgac gttaaagtca aaaacgtgaa catcgtgaac
    36061 gtacgtgcga aattcaaacg tatgggcaaa tatgcaggat atacaaaaaa acgtcgcaaa
    36121 gcgatcgtta cattgactga agattcaaaa gaaatccaat tattcgaagc tgctgaataa
    36181 gcactaatag ataactcatt aaattaggag ggaaaaacgt ggcgattaaa aagtacaaac
    36241 ctacctcaaa tggtcgtcgt aacatgacag gttctgattt tgctgaaatc acaacatcaa
    36301 cacctgaaaa atcattgtta caaccattga aaaaccatgc tggacgtaac aacaacggcc
    36361 gcatcactgt acgtcatcaa ggtggcggac acaaacgcca atatcgcgtg atcgacttca
    36421 aacgtaacaa agataacgtt gttgcaactg ttcaaacgat cgaatatgat ccaaaccgtt
    36481 cagctaacat cgcgttagta cactatgaag atggagtgaa agcttacatc ttagcaccta
    36541 aaggactaga agtgggtatg aaacttgttt caggtccaga agcagatatc aaagtcggaa
    36601 atgctcttcc attagaaaac attccagttg gtactgttat ccacaacatt gaaatgaaac
    36661 ctggtaaagg tggacaattg atccgttctg ctggtacaag tgctcaagta cttggtaaag
    36721 aaggcaaata cgtattgatc cgcttgaact ctggtgaagt tcgtatgatc ttagctgcat
    36781 gccgtgctac aatcggttct gtaggtaacg aacaacacga attaatcaac atcggtaaag
    36841 ctggacgctc tcgttggatg cgtaaacgcc caactgtacg tggtagcgta atgaacccta
    36901 atgatcaccc acacggtggt ggtgaaggta aagctcctat cggacgtaaa gcaccagttt
    36961 ctccttgggg tcaaccagct cttggttaca aaactcgtaa caaaaaagct aaatcagaca
    37021 aacttattgt tcgtcgtcgc aaaaataaat aattactttg accatgtgtc gcacattttg
    37081 agaggaggtt caccatgggt cgtagtttaa agaaaggacc ttttgtcgat gagcatttaa
    37141 tgaaaaaagt tgaagctcaa gcaggggttg aaaagaaaaa agttatcaaa acttggtcac
    37201 gccgttcaac agttttccca agttttgttg gatataccat cgcagtttat gatggacgta
    37261 aacatgtacc agtttacatc caagaagaca tggtaggaca caaattaggt gaatttgcac
    37321 ctacaagaac ttatcgtgga catgctgctg acgataagaa aacgaaacgc taatttgagg
    37381 ggaggataaa tccaaatggc agaacaaatc acatcagcta aagcaactgc aaaaacagtt
    37441 cgcacttcac ctcgaaaagc ccgtttagta attgacctta tcagaggtaa aagtgtcgca
    37501 gatgcaattt caatcttgaa attcacacca aacaaatctg ctggaatcat tgaaaaagta
    37561 ttgatgtcag cagttgcgaa tgcagaaaat aactttgact tagatgttga aaacttggta
    37621 gtaagcgaag cttttgttaa cgaaggacct acaatgaaac gtttccgtcc tcgtgcaaaa
    37681 ggatcagctt ctccaatcaa caaacgtaca agtcatatca cggtagtcgt atcagaaaaa
    37741 taaggaggga taatcagtgg gtcaaaaagt acatccaatt ggaatgcgtg taggcatcat
    37801 ccgcgactgg gatgctaaat ggtatgctga aaaagagtat gctgagttct tacacgaaga
    37861 tttaagaatc cgtaaattta tttcaactaa acttgctgat gctgctgtat caacagttga
    37921 aatcgaacgc gcaacaggtc gcgtaaacat ttcaattcac acagctaaac caggtatggt
    37981 tattggtaaa ggcggttctg aagtcgaaag cctaagaaaa gaattaaaca aattaactgg
    38041 taaaagaatc catatcaata tcgtggaaat caaaaaacca gatctagatg caaaattagt
    38101 aggtgaagga atcgcacgtc aattagaaaa ccgtgttgct ttccgtcgcg ctcaaaaaca
    38161 agccatccaa cgctcaatgc gtgctggcgc tcaagggatc aaaactcaag tatctggtcg
    38221 tctaaacgga gcagatatcg ctcgttcaga aggatactca gaaggaactg ttcctcttca
    38281 tactttacgt gcggacattg attacgcatg ggaagaagca gatacaacat acggaaaact
    38341 aggagttaaa gtgtggatct atcgtggaga aattcttcca actaaaaaaa acactgagaa
    38401 aggagggaaa taatcatgtt agtacctaaa cgtgtaaaac accgtcgtga attccgcgga
    38461 aaaatgcgcg gggaagctaa aggcggaaaa gaagtagcat tcggtgaata cggtttgcaa
    38521 gctgttgatt cacattggat cacaaaccgc caaatcgaag ctgctcgtat cgcaatgact
    38581 cgttacatga aacgtggtgg gaaagtatgg attaaaattt tccctcacaa atcttatact
    38641 gccaaagcaa ttggggtacg tatgggttct ggtaaagggg cacctgaagg atgggttgca
    38701 ccagtaaaac gtggtaaaat catgtttgaa atcgcaggcg ttcctgaaga agtagctcgt
    38761 gaagcgttac gtctagcttc tcacaaatta ccaatgaaaa ctaagatcgt aaaacgtgag
    38821 gaaatgggtg gtgaatcgaa tgaaggttaa agaaatcaga gaattaacca ctgccgaaat
    38881 gcttgatcaa gaaaaacaat taaaagaaga attgtttaat cttagattcc aattagcaac
    38941 aggtcaatta gaaaacactg cacgtattaa agaagtacgt aaatcgattg cacgcatcaa
    39001 aactgtgttg cgtgaacaag ctaagtaatt atggaaggag gccattaagc gtatgactga
    39061 aggaagaaat caacgcaaag tttatcaagg tcgcgtggtt tcagacaaaa tggataaaac
    39121 aattaccgtt gctgtcgaaa caaagaaaaa ccaccctatc tatggcaaac gcatgaacta
    39181 ttctaagaaa tacaaagtgc atgatgaaaa caacacagca aaagttggcg acatcgtgag
    39241 aattatggaa actcgtccat tatcagctac aaaacgtttc cgtttgttgg aagtagtcga
    39301 agaagcagtt attatctaat aacacgagat tttcctatat agattgaatg caaaatctga
    39361 aaggaggata cacaacgtga tccaagcaga aagtcgttta aaagtcgctg ataactcagg
    39421 cgcacgtgaa attttgacga tcaaagtcct tggtggatct ggtcgtaaaa ctgctaacat
    39481 cggtgatgtg atcgttgcta cggttaaaca agcaacgcca ggtggggttg tcaaaaaagg
    39541 tgacgtcgtt aaagccgtta tcgttcgaac aaaatcagga gctcgtcgta cagacggttc
    39601 atatattaaa tttgatgaaa atgctgcagt gattattcgt gatgataaaa gccctcgtgg
    39661 aacacgtatc tttgggccag ttgcacgtga actacgtgaa aacaacttca tgaagatcgt
    39721 ttcactagca ccagaagttt tataatcttg agacacttat caaaggaggt gcgaaacagt
    39781 tatgtttgtt aaaaaaggcg ataaagttaa agttatcact ggaaaagaca aaaacaaaga
    39841 aggcgttgta ctagcagcgt tcccgaaaaa agataaagtt attgtcgaag gtgtaaatgt
    39901 cgttaaaaaa caccaaaaac cttctcaagc tgctcctcaa ggtggaattg ttgagatgga
    39961 agcgccaatt catgtttcta acgtgatggt ggttgactct actggtgtag ctggccgagt
    40021 tggctacaaa gaagtagacg gcaaaaaagt ccgtgtttct aaaaaaaccg gtgaagtttt
    40081 agataaataa aagtttaagg aaggagggtc ttactgaatg aaccgcctaa aagaaaaata
    40141 tcttaaagaa gtaactccat cattgatgga aaaatttaac tatagctctg ttatgcaaac
    40201 acctaaagtt gaaaaaatcg tcatcaacat gggtgttggt gatgcggtat caaacgctaa
    40261 aaacttagat aaagcagttg aagaattaac tttgatcact ggtcaaaaac caatgattac
    40321 aaaagctaaa aaatcaatcg ctggattccg tttacgtgaa ggaatgccaa ttggtgcaaa
    40381 agttacattg cgcggagaaa gaatgtacga attcttagac aaattggttt ctgtttcact
    40441 accacgtgta cgtgacttcc atggtgtaag taaaaaagcc tttgacggtc gtggaaacta
    40501 tactttaggt attaaagaac aattgatctt cccagaagta gattatgatt tagtagataa
    40561 agtacgtggt atggacatcg ttattgtaac aacagcaaac tctgatgaag aatctcgtga
    40621 gttgttgaca caattaggca tgccattcca aaaataatta aaggaggcga actacgtggc
    40681 taaaaaatca atgattgcta aaaacaaacg tcctgcaaaa ttttcaacac aagcttatac
    40741 tcgttgcgaa cgttgcggac gtccacattc agtttatcgt aagttccatc tttgccgtat
    40801 ttgcttccgc gaacttgcct ataaaggtca aattcccggc gtgaagaaag ctagctggta
    40861 aaaagtaaat tcgcataaga aggaggtaaa agatcaatgg tcatgacaga tccaattgca
    40921 gattttctaa ctcgtatccg taacgcaaac atggttaaac atgagtcttt agaagtacct
    40981 gcatcaaaaa ttaaacgtga catcgctgaa atcttgaaac gtgaaggttt cgttcgtgat
    41041 gttgaataca tcgaagatga caaacaaggc gtgatccgtg ttttcctaaa atttggtaaa
    41101 aacggcgaac gtgttatcac aaacttaaaa cgtatctcta aaccaggttt gcgtgcttat
    41161 gtcaaagctg acgaagtacc aaaagtattg aacggtttag gtatcgcaat catttcgact
    41221 tctgaaggtg ttgtcactga taaagaagct cgtgaaaaaa atatcggcgg cgaagttatc
    41281 gcttacgtat ggtaatcaag tagaatctac aaggaggtgt ctttaagtga gccgtatcgg
    41341 aaataaagtc gttgttatcc ctgaaggagt aacagttact caagacggaa acaacgttac
    41401 agttaaagga ccaaaaggtg aattgactcg tactttttca gcagatatca aaatgaatat
    41461 cgaaggaaac gaagtaactt tcactcgtcc aaacgacagt aaagaaatga aaacaattca
    41521 tggaacaact cgtgcgaact tcaacaacat ggttgtaggt gtcagcgaag gtttccaaaa
    41581 agcgttagaa ctaatcgggg ttgggtaccg tgcacaatta caaggatcaa aacttgtatt
    41641 gaacgttggt tactcacatc cagtagaaat cacaccacca gctggagtaa ctgttgaagt
    41701 accagctaac acacaagtaa ttgttaaagg tgcgaacaaa gaagaagttg gcgaattagc
    41761 tgcaaacatt cgtggaactc gtcctccaga accttataaa ggtaaaggta ttcgttatgt
    41821 tggcgaatat gtacgccgta aagaaggtaa aactggtaaa taatcgttta gaaaaattgc
    41881 atagaagcca gcagcaggaa aaaggcattt ggctgaagtt taccttcaaa aaaatacatt
    41941 tttacttgtg ccgcagcttc tatttttcga aacgtattta ctgcttgaaa tagtagtaca
    42001 ataaaaataa agaggtgaca attgtgatta caaaaccaga taagaacaaa acacgtcaaa
    42061 agagacatcg tcgtgtacgt aacaatatct ctggtactgc tgagcgccca cgcttgaacg
    42121 tttttcgttc taacaaaaac atctacgctc aaattattga tgacgtagcg ggtgtaacgc
    42181 tagcaagtgc ctctaccttg gataaagaaa tttcaggtgg aacaaaaaca gaagcagcac
    42241 aagctgtcgg taaactagtt gctgaacgtg cagctgaaaa aggcattaaa gtagtagtct
    42301 ttgaccgtgg tggatacctt taccatggcc gtgtgcaagc tttagctgaa gctgctcgcg
    42361 aaaatggact agaattttag aagaaggagg aataccattc atggtttata tcgatccaaa
    42421 acatttggaa ttagaagacc gcgttgttgc aattaaccgt gtaacaaaag ttgttaaagg
    42481 tggacgtcgt ctacgttttg ctgctttagt tgttgtcggt gacaaaaacg gacacgtagg
    42541 gtttggtaca ggtaaagctc aagaagttcc tgaagcaatc cgtaaagcaa ttgaagatgc
    42601 gaagaaaaac ttagttgaag tacctatggt tggttcaaca atcccacacg aagtaatcgg
    42661 tgcttttagt ggtggtagaa tcctaatgaa acctgctgta gaaggttctg gggttgctgc
    42721 tggtggacca gttcgtgccg tattagaatt agctggggta gcagatatca cttctaaatc
    42781 tttaggctca aatacaccaa tcaacgttgt tcgcgcaaca gttgaaggtt tgaaacaatt
    42841 gaaacgtgca gaagaagttg cagaacttcg cggcaaatca gttgaagaat taatcggata
    42901 aggaggacaa caataatggc tgaattaaaa gttactttaa aacgcagtat tatcggacgt
    42961 cctcaaaacc aacgcgatac agttaaagcg ttaggtctta caaagattaa cagttcagtg
    43021 gttaagcctt ctaatgaagc aattaaaggc atgatcaata ctgtttctca cctagtggac
    43081 gttgaagaaa tttaatttag aaaaatcata actagaacat taaggaggtg ccaaacctat
    43141 gaaacttcat gaattaaaac ctgctgaagg ttcacgccaa gtacgtaacc gtgttggtcg
    43201 tggtacttca tctggtaacg gaaaaactgc cggacgcgga caaaaaggac aaaaagctcg
    43261 ttcaggcggc ggtgtgcgtt taggatttga agggggacaa acaccattgt tccgtcgttt
    43321 accaaaacga ggattcacaa acgtgaaccg taaagactat gcagtcatta atcttgatgt
    43381 attgaaccgc tttgaagacg gagctgaagt aactcctgtt gctttagttg aagctggaat
    43441 cgtgaaaaac gaaaaagctg gaatcaaagt attagctaat ggggaactaa acaagaaatt
    43501 aactgtgaaa gcagcaaaat tctctaaagc agctcaagaa gcaattgaag cggctggtgg
    43561 atcaatcgag gtgatctaat gtttagacta ttaaaagatg cttttaaagt aaaagacatt
    43621 agatcgaaaa tcctatttac tgtatttgtg ttgtttgtat ttcgcctagg tgcacatatt
    43681 actgtaccag gcgtaaatgc tgcttcattg caaaaccttt cttctttgcc atttttcaac
    43741 atgttgaact tggtaagtgg tagtgcgatg caaaactttt ccattttctc aatgggggtt
    43801 tcaccataca ttactgcttc tatcgtcatt cagcttcttc aaatggacat cgtgcctcga
    43861 tttgttgaat ggtcgaaaca aggggaagtt ggtcgtaaaa aactgaatca agcgactcga
    43921 tatctgacat tagtattggc atttatccaa tctgtcggta tcactgctgg attcaatcaa
    43981 tacgctcaat taggtttcgt gaataatcct aacgtagcaa cttacgtaac gatcggtttg
    44041 attctaacag ccggtacgat gtttgttact tggctaggag aacagatcac agaaaaaggt
    44101 atcggaaatg gcgtatcaat gatcattttt gcaggtatca tttctagatt gcctgaaagt
    44161 atcagagaaa tttatgaaga ttactttata aatattgatc cttcagacat atggaaatct
    44221 gctatttttg tggccatttt actagtagcg gtcttgttga tcgtgacatt cgtaacattt
    44281 ttccaacaag ctgaacgcaa gatcccgatc caatatacga aacgcgtagc tggcgcgcct
    44341 acaagcagct atctgccgtt gaaagtaaat gcagctggag ttattccagt tatctttgct
    44401 agttcgttta ttgctacgcc aaatgctgtt ttacaagcat tgagcggtag attcggttct
    44461 gaaaactggt acaaagtgat gatggagatc tttaactata atacagcacc tggtgctacg
    44521 atttatacga ttctgatcgt tgccttcact ttcttctacg cctttgttca agtaaaccct
    44581 gagaaattag cggaaaactt gcaaaagcaa ggaagttata taccaagcgt acgccctggt
    44641 aaggggactg aagagtatgt ctcaagatta ctgatgcgtt taagtacagt tggtgcagtc
    44701 ttccttggtt tagtggcatt gcttccaatc attgctcaaa tggtttggga tcttccacaa
    44761 tcaatcggat taggcggtac aagcttactg atcgttatcg gtgtagcttt agaaactgcg
    44821 aaacagttag aaggcttaat gctgaaacgt aaatatgttg gattcattga gtaagaaagc
    44881 gtgttgaggt ttcccctcaa cacacgttct cacacattta ttaggaggga aaacaatgaa
    44941 cctcatttta atggggctgc caggcgcagg taaaggaaca caagctgaaa aaatcattga
    45001 tgcttatggt attccgcaca tctcaacagg agatatgttc cgcgcagcga tgcaaaacga
    45061 aacagctctt ggcctggaag caaagtctta tatggataaa ggtgcattag ttcctgatga
    45121 agtaacaaat ggaatcgtga aagagcgact agctgaacca gatacagaga aaggcttctt
    45181 attagatggc tttccacgta cgctagatca agcaaaagct ctggacgcaa tgctaaaaga
    45241 tttgaataaa aaaattgatg ctgtcatcga tatccatgtt ggtgaagaaa tcttggtcga
    45301 acgtctagcc ggccgattta tctgcagcaa ttgtggagca acttaccata aaattttcaa
    45361 ccctacaaaa gttgaagaca catgtgatcg ttgcggtggg catgaattct atcaaagaga
    45421 agatgataaa cctgagacgg ttaaaaatcg tctggctatc aacatcaaaa atagtgaacc
    45481 aatcttggct tattataaag aacaaggttt gctaaacact attgatggtg ctcgtgaaat
    45541 cgatgctgta tttgcagatg tacaaaaaat cattgaaaaa tagcacgatt gcccgtagcc
    45601 gacttgcatt atgcagttac tcttgtcaag ttcaataaat tatgatagaa tatgtcagtc
    45661 cgacttctag agaatcctct agatgatcca ttttgagaat tttgaggtgg gaactgcagt
    45721 ttccgccatt ttatcgtgtg aaaatgcgaa acaagtacaa ggaggtactg tgcgtggcaa
    45781 aagaagatat gattgaagtc gaaggtacag tcgtcgaaac tttgccgaat gcaatgttta
    45841 aagttgaact agaaaacgga caccaagttc ttgctactgt ttcaggtaaa attcgtatgc
    45901 attacatccg tatcttacct ggtgacaaag taacggttga attgtctcca tacgatctta
    45961 accgtggccg tattacttat cgctttaaat aattgtactc cgtaaaacta caggaggtat
    46021 tatcatgaaa gtaagaccat cagtaaaacc aatgtgcgaa cattgtaaag taattcgtcg
    46081 taaaggacgc gttatggtga tttgcccagc aaatccaaaa cataaacaac gtcaaggata
    46141 attggaggtg ctataaatat ggctcgtatt gcaggagtag atattcctcg tgataaacgt
    46201 gtagtagttt cacttactta tatctatggt attggtaaca ctactgctaa aaagatctta
    46261 gctgatgctg gtgtatctga agatgttcgc gttcgtgaat taacgaatga acaaacagat
    46321 gctatccgtg cagaagtaga taaattaaaa gttgaagggg atcttcgtcg tgaagtgaac
    46381 ttgaacatca aacgtttgat ggaaatcggt tcttaccgtg gaatccgtca tcgccgtgga
    46441 ttgcctgttc gtggacaaaa cacgaaaaac aatgcacgta ctcgtaaagg cccagctcgc
    46501 gctatcgctg gcaagaaaaa ataattctta agtgaaggag gttaaaactt catggcagca
    46561 aaaaaagtga atcgtaaacg ccgtgtgaag aagaatatag aagcaggtat tgcacatatc
    46621 cattctacat tcaataacac tatcatcatg atcactgata ctcacggaaa tgcattagcg
    46681 tggtcatcag ctggttcatt aggtttcaaa ggaagcaaaa aatcaactcc ttttgccgct
    46741 caaatggcag cagaaactgc tacaaaagca gcaatggaac acggattaaa gactgttgaa
    46801 gtaacagtta aaggacctgg ttctggacgt gaagcagcaa ttcgttcatt acaagctaca
    46861 ggtttagaag tgactgcaat tcgtgacgtg actccagttc ctcataatgg tgcccgccct
    46921 ccaaaacgcc gtcgtgttta atgagtgccg ctcattttga gttttatcag taacgtcatt
    46981 gaacaagaca cttttcgttt tgaaaggggt aaagataaga atgattgagt tcgaaaaacc
    47041 aagaatcgca aaaattgatg aagaaaaaga ttatggcaag ttcatcgttg aacctcttga
    47101 aagagggtat gggactactt tagggaattc cctacgtcgt attttattat cttctttgcc
    47161 aggtgctgcc atcactaata ttcaaatcga tggtgtactg catgaattct ctaccatcaa
    47221 aggtgttaga gaagacgtca ctcaaatcat cttgaacatt aaaggcttag cgctgaaaat
    47281 gtatggccaa gaagaaaaaa cccttgaaat cgatattacc ggacctgcta cagtaactgc
    47341 tggcgatatt atcgttgata gtgatgtaga aattcttaac aaagatatgt acatctgtag
    47401 tgtctctgaa ggagctacgt tccatgctcg cttaacagtg aaacctggac gtggttatgt
    47461 tcaagcagat gaaaataaaa aagaagatat gccaattggc gttcttccag tcgattcaat
    47521 ctacacacct gttcgtcgtg tgaactatca agttgagaac acacgtgttg gacaccgtga
    47581 agattttgat aaattgacga tggagatttg gacagatggt tcaattgaac cgttggaagc
    47641 aatgagtttg tctgcgaaaa tcatgactga acatttggat atttttgtga acttaactga
    47701 tgaagcaaaa aatgctgaaa tcatggtcga aaaagaagaa acacaaaaag aaaaaatgtt
    47761 ggaaatgacg atcgaagagt tagacttgtc tgttcgctct tacaattgtt tgaaacgtgc
    47821 aggaatcaat actgtacaag aacttacaaa taaatctgag cctgaaatga tcaaagtccg
    47881 taacttagga cgtaaatcat tagaagaagt taaagcaaaa ttgcacgact taggtttagg
    47941 tttacgtaaa gaagattaaa accttttacg aaggagggaa accaacgtga gttatcgtaa
    48001 actaggacgc acatctagcc aacgtaaagc gatgttgcgt gatttaacaa ctgatttatt
    48061 aatcaacgaa cgtattgtga ctactgaagc tcgtgcgaaa gaagttcgct caactgcaga
    48121 aaaaatgatc actttaggta aacgcggaga tttgcatgca cgtcgtcaag ctgcggcttt
    48181 cgtacgtaat gaagttgcca gcgtgcgtga agaaaatgag gacatcgtca ttgaatcagc
    48241 tttacaaaaa ctgttcaacg atattgcacc tcgttacgca gaacgtcaag gcggatacac
    48301 acgcattttg aagacagaac caagacgcgg tgatggtgca ccaatggttg tcttagaact
    48361 tgtctaaaat cacactaata acatcacttt atagatgatt cttttagagt gttatgatgt
    48421 tggaagatat ttttccgagt ctagctcagc gctgctcctc aagcgaatat cgcttgggga
    48481 gtaagcactc atcataaagt gtttttttat accttcaatt acctgtcatg acagatatgt
    48541 ttgaaggcat aaaaaaacat tttaagcgag aaataattga aaattttgtc tttttttgat
    48601 agtgttagta ggaactttag gatgaagaag gagtaatcat ggagccaatt attgaattag
    48661 aaaagataaa ttataaatac cagccagatg atcttcgccc cgcattgaaa gatgtctcct
    48721 ttacaattga taaaggtgag tggatcgcta ttattggaca caatggttca ggaaaatcga
    48781 cacttgccaa aacgatcaat ggattgcttc tgccagaatc agggatcgtg aaagtgggaa
    48841 atcaaatatt ggatgaagaa aatatttgga cgatccgaca gatggttgga atggtttttc
    48901 aaaatccaga caatcagttt gttggttcga cagtagaaga tgacgttgct tttggtcttg
    48961 aaaatcaagg gattcccaga gaagaaatgc tagtgcgtgt gaaagatgca cttgagaaag
    49021 ttagaatggc tgagtttgct tcacgcgagc ctgctcgttt atcaggaggg caaaaacaac
    49081 gtgttgccat tgccggcgtg gttgccttac gacctgatat cattatttta gatgaagcaa
    49141 ccagtatgct agatccagaa ggtcgtgaag aagtgatctc tacgatcaag aagatcaaag
    49201 aagaaagtca attgaccgtt atatctatca ctcatgatat tgatgaagca gctaatgcca
    49261 atcggatact agtgatgaga caaggcgaac ttgtccgtga aggtacacca aaagaaattt
    49321 tttctgctgg tcctgaactg atcgacttag gtctagatct accatttcct gaaaaactga
    49381 agagcgcact gaaagaacgt ggggttgatg tgcccagtga atatatgaca gaagaaagga
    49441 tggtggattg gttatggaca tccgttttga acaagtagac ttcacttatc agccgaatac
    49501 accatttgaa caaagagcct tatttgacat caatatgacg atcaaagaaa acagttatac
    49561 tgcattagta gggcataccg gaagcgggaa atcaacctta cttcagcatt tgaatgcgct
    49621 tgttaagcca acaagcggta cagtccatat tggagaacga gatattcagc cggatacgga
    49681 taataagaat ttaaagccca tccgaaaaaa agttggcatc gtctttcagt ttccagaagc
    49741 acagctattc gaagaaacgg tagcaaaaga cattgctttc ggtcctaaaa actttggtgt
    49801 cagcgaagaa gaagcactag tcctagcaaa agaaacatta gaacaagttg ggctggatga
    49861 aagctatttg gaacgttcgc catttgaact ttcaggaggg caaatgcgta gagtagcaat
    49921 cgctggtgtg cttgccatga ggccggaagt acttgtattg gatgaaccaa cggcaggact
    49981 tgatccccag ggtagaaaag aaatgatgga gatgttttgg cgtcttcata aggaacagca
    50041 gatcactatt gtattagtta cgcatttaat ggatgatgta gccaattttg cagattatgt
    50101 ctatgtgttg gaaaagggac gaatcgttaa tagcggggag ccgcaagagg tctttcaaaa
    50161 cattgaatgg ctaaaagaaa aacaactagg cgtacctact gccaccgagt ttgcagaaga
    50221 attaatggct aaagggatga actttgctac cttgcctttg acagctgaag aattagcaga
    50281 cgctatcgtc cattatgctg gaggaaatgc ccatgatgaa taaattgata tttggtcgtt
    50341 atatgcctgg agattccttg atccatcgat tggatccccg agctaaatta ttagccagtt
    50401 tttattttat cggaatcatt tttttagcaa ataattggga aacgtacctt ttgttggcat
    50461 ttttcacatt actttctgtc ttcttgtcaa aagtagattt gaaatttttt atccgaggca
    50521 ttcgcccatt gatctggcta atcctcttca ctgtagcttt gcaggtcttg tttacaagtg
    50581 gcggagaaac ttattggagt tggggtgttt tccatatcac tgaatttggt gtgatgaacg
    50641 gcctctttat tttctgtcga ttcgtgttga tcatttttat gtcgactcta ctcacgctta
    50701 ctacaccgcc tttggaatta tcggatgcga ttgagtactt gcttcgtcca ttgaaagccg
    50761 tacggttccc agtccacgaa atttcattga tgctgtcgat tgctcttcgt tttgtgccta
    50821 cattgatgga cgaaacagaa aagatcatga acgctcagcg cgctcgcggt gttgattttg
    50881 gtgaaggaaa cctagttcag aaaatgaaag cagtcattcc tttgctgatt cctttatttg
    50941 tcagcagctt caaccgtgcg gaagatcttg cgactgctat ggaagcaaga ggctacaaag
    51001 gtggggaagg aagaacgaaa tatcgtgtat tgcattggca taatagagac acgatggtta
    51061 tcattgcttt tgtattatta actgtgctac tgatcttttt gagaggataa acgttcagtg
    51121 ggccgctatc tattcttttc ttgctgatct gtttgcaaga aaggatgcga gcagtgtatg
    51181 acaagaaata ttagggtctg tggcagacta aggtcacagg cctctttctt ttatctgaaa
    51241 agaaagaacg aaaaaatttt ataaaaaaat cattattcca gataaccttt taggaacaaa
    51301 ggagagaaaa acatggtacg ttacaaagca atcattgcat acgacggaac aaattttaat
    51361 ggattccaaa aacagccaaa tgggcgtact gttcaagaag aagtagaaaa aacattacaa
    51421 aaaatggcaa atggaaaaga aatcacggtc tttggttcgg gaaggacaga tgccggagta
    51481 catgcgattg ggcaagtaat ccattttgat tatccagagg agcgtccgtt agaacgaatg
    51541 cgtttcgcgc tagataccca gtcaccagag gatattgctg taagacaagt agaaattgtt
    51601 tcagaagact tccacgcaag atatttggtc aaagaaaaga cgtatcaatt tcgagtggat
    51661 atagggaaac cacgcagtcc ttttcgacgc cattatgcaa gttattttcc ttaccctctt
    51721 gatttggaaa aaattcagcg ggcgcttcct gatttattag ggactcacga tttcacctct
    51781 ttttgtgcat caggaagctc gatagaggat aaagtacgta cgatctacga ggctaagatg
    51841 gaggtaaatg aaacaaaaga cgaattgttg tttacattta ggggaaatgg cttcttgtat
    51901 aagatgatac ggatcttagt aggaacgctt ttgaaaattg gcaatggccg attgcctgaa
    51961 gatagtatcc cagatattat tgcgaaaaaa gatcgtaatg cagcaggacc tacagcacat
    52021 cctgaaggac tttatttgta tgaagtgaaa tatgaataag aggttgtgac agaagtggtc
    52081 agcttcaaga aataagaagg aatttacgaa aattacttct caaaattttg caaaatttcg
    52141 gcttattttc gaaggagcta cttctggaac accgtgaatc agaggttgtg aaaaagctgc
    52201 cctgcatcaa gtaataagaa caaccaaaca aaaatagctc gtcatatttt tcgtttggtt
    52261 gggcttatta ccgcagatgc aagcttttga acaccgttta aggaagaggt tgtgaaatca
    52321 gcgttttgac ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    52381 tgttgagcta ataccgaagg tcagctgatt gaacaccgtt taagaaagag gtcgtgagcc
    52441 tgtcatttaa ctctgagtca caagtaacaa tttcaaaaaa cagcttctca tctttgagga
    52501 atatcgtgac ttgtggacga ggcgttgata agtgaatcat ctttttgtta cctatttttt
    52561 ctgtaaccaa aaaacgatgg aacgttgttt tgattgccct taatgaggac agtcggaaca
    52621 ggtttgtcat ttttat
//
LOCUS       DACEQH010000014        52141 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.014, whole genome shotgun sequence.
ACCESSION   DACEQH010000014 DACEQH010000000
VERSION     DACEQH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.014"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            330..1175
                     /locus_tag="IW833_000851"
     CDS             330..1175
                     /locus_tag="IW833_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1306987.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="IW833_000852"
     CDS             1456..2676
                     /locus_tag="IW833_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ1306988.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="IW833_000853"
     CDS             2673..5393
                     /locus_tag="IW833_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1306989.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="IW833_000854"
     CDS             5377..6321
                     /locus_tag="IW833_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1306990.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="IW833_000855"
     CDS             complement(6390..7400)
                     /locus_tag="IW833_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1306991.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="IW833_000856"
     CDS             complement(7580..7924)
                     /locus_tag="IW833_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1306992.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="IW833_000857"
     CDS             complement(7917..8351)
                     /locus_tag="IW833_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ1306993.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="IW833_000858"
     CDS             8489..9226
                     /locus_tag="IW833_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1306994.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="IW833_000859"
     CDS             9219..10430
                     /locus_tag="IW833_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1306995.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10637..11416
                     /locus_tag="IW833_000860"
     CDS             10637..11416
                     /locus_tag="IW833_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ1306996.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="IW833_000861"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="IW833_000861"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ1306997.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IW833_000862"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IW833_000862"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ1306998.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="cpaF"
                     /locus_tag="IW833_000863"
     CDS             14132..15061
                     /gene="cpaF"
                     /locus_tag="IW833_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ1306999.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="IW833_000864"
     CDS             15018..16067
                     /locus_tag="IW833_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ1307000.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="IW833_000865"
     CDS             16064..16342
                     /locus_tag="IW833_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ1307001.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="IW833_000866"
     CDS             16317..16787
                     /locus_tag="IW833_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ1307002.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="IW833_000867"
     CDS             16804..17106
                     /locus_tag="IW833_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307003.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="IW833_000868"
     CDS             17123..17494
                     /locus_tag="IW833_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ1307004.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="IW833_000869"
     CDS             17466..17825
                     /locus_tag="IW833_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307005.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="IW833_000870"
     CDS             17997..19004
                     /locus_tag="IW833_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1307006.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="IW833_000871"
     CDS             19029..20213
                     /locus_tag="IW833_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ1307007.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="IW833_000872"
     CDS             complement(20650..22233)
                     /locus_tag="IW833_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1307008.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="IW833_000873"
     CDS             complement(22811..22954)
                     /locus_tag="IW833_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ1307009.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23176..23655)
                     /locus_tag="IW833_000874"
     CDS             complement(23176..23655)
                     /locus_tag="IW833_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1307010.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23728..24012)
                     /locus_tag="IW833_000875"
     CDS             complement(23728..24012)
                     /locus_tag="IW833_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307011.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24090..24284)
                     /gene="ssrS"
                     /locus_tag="IW833_000876"
     ncRNA           complement(24090..24284)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IW833_000876"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24356..25642)
                     /locus_tag="IW833_000877"
     CDS             complement(24356..25642)
                     /locus_tag="IW833_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ1307012.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25780..26451
                     /locus_tag="IW833_000878"
     CDS             25780..26451
                     /locus_tag="IW833_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ1307013.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26417..26905
                     /locus_tag="IW833_000879"
     CDS             26417..26905
                     /locus_tag="IW833_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ1307014.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26918..27865
                     /gene="prmA"
                     /locus_tag="IW833_000880"
     CDS             26918..27865
                     /gene="prmA"
                     /locus_tag="IW833_000880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ1307015.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27866..28630
                     /locus_tag="IW833_000881"
     CDS             27866..28630
                     /locus_tag="IW833_000881"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ1307016.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITKITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28863..31076
                     /locus_tag="IW833_000882"
     CDS             28863..31076
                     /locus_tag="IW833_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ1307017.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31087..31533
                     /locus_tag="IW833_000883"
     CDS             31087..31533
                     /locus_tag="IW833_000883"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ1307018.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31704..32963
                     /locus_tag="IW833_000884"
     CDS             31704..32963
                     /locus_tag="IW833_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ1307019.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33052..33999)
                     /locus_tag="IW833_000885"
     CDS             complement(33052..33999)
                     /locus_tag="IW833_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1307020.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34393..35685
                     /locus_tag="IW833_000886"
     CDS             34393..35685
                     /locus_tag="IW833_000886"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ1307021.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35700..37469
                     /gene="aspS"
                     /locus_tag="IW833_000887"
     CDS             35700..37469
                     /gene="aspS"
                     /locus_tag="IW833_000887"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ1307022.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37578..38453
                     /locus_tag="IW833_000888"
     CDS             37578..38453
                     /locus_tag="IW833_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1307023.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38719..40602
                     /locus_tag="IW833_000889"
     CDS             38719..40602
                     /locus_tag="IW833_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1307024.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40677..40961)
                     /locus_tag="IW833_000890"
     CDS             complement(40677..40961)
                     /locus_tag="IW833_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1307025.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40951..41685)
                     /locus_tag="IW833_000891"
     CDS             complement(40951..41685)
                     /locus_tag="IW833_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ1307026.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41814..42437
                     /locus_tag="IW833_000892"
     CDS             41814..42437
                     /locus_tag="IW833_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ1307027.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42629..43750
                     /locus_tag="IW833_000893"
     CDS             42629..43750
                     /locus_tag="IW833_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ1307028.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43757..46885
                     /locus_tag="IW833_000894"
     CDS             43757..46885
                     /locus_tag="IW833_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ1307029.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46904..50098
                     /locus_tag="IW833_000895"
     CDS             46904..50098
                     /locus_tag="IW833_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1307030.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50244..50951)
                     /locus_tag="IW833_000896"
     CDS             complement(50244..50951)
                     /locus_tag="IW833_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1307031.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51716..52114
                     /gene="spxA"
                     /locus_tag="IW833_000897"
     CDS             51716..52114
                     /gene="spxA"
                     /locus_tag="IW833_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ1307032.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        17113 a         9069 c        10787 g        15172 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc ttgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggctt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat
    23041 tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg
    23101 cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg
    23161 caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc
    23221 agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc
    23281 aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg
    23341 attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga
    23401 ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt
    23461 tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga
    23521 acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac
    23581 cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt
    23641 gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta
    23701 gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa
    23761 tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa
    23821 gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc
    23881 gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc
    23941 ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga taatcattaa
    24001 aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa
    24061 gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg
    24121 tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa
    24181 atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt
    24241 tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca
    24301 tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt
    24361 tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt
    24421 catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat
    24481 attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt
    24541 tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt
    24601 tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg
    24661 gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca
    24721 atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac
    24781 ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct
    24841 caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt
    24901 gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc
    24961 tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag
    25021 gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg
    25081 cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc
    25141 gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt
    25201 tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct
    25261 cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct
    25321 gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt
    25381 ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc
    25441 gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag
    25501 gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt
    25561 ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc
    25621 gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta
    25681 tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt
    25741 atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata
    25801 gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat
    25861 ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac
    25921 ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg
    25981 gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg
    26041 aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc
    26101 gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc
    26161 aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct
    26221 atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat
    26281 gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt
    26341 gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg
    26401 ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga
    26461 aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga
    26521 gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg
    26581 gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca
    26641 gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg
    26701 gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg
    26761 gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca
    26821 ctgggataat caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac
    26881 ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt
    26941 agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag
    27001 tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga
    27061 gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt
    27121 tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt
    27181 acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag
    27241 caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac
    27301 ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact
    27361 agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc
    27421 actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt
    27481 gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga
    27541 agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca
    27601 tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa
    27661 cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac
    27721 aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat
    27781 gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat
    27841 tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc
    27901 aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat
    27961 ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat
    28021 cacgaagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa
    28081 ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg
    28141 gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc
    28201 ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat
    28261 tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga
    28321 aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga
    28381 ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc
    28441 agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga
    28501 ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga
    28561 aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc
    28621 tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc
    28681 aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa
    28741 gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat
    28801 tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc
    28861 ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca
    28921 tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg
    28981 acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta
    29041 tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg
    29101 ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta
    29161 tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc
    29221 tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg
    29281 tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc
    29341 aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga
    29401 tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt
    29461 attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg
    29521 gtactgttga agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg
    29581 gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca
    29641 acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg
    29701 ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata
    29761 ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag
    29821 gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg
    29881 ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga
    29941 cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt
    30001 ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac
    30061 caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta
    30121 tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc
    30181 ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg
    30241 gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac
    30301 gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca
    30361 aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag
    30421 aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg
    30481 gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa
    30541 aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg
    30601 acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa
    30661 ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta
    30721 aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa
    30781 atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt
    30841 atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc
    30901 aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata
    30961 aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg
    31021 tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg
    31081 acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa
    31141 gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg
    31201 caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa
    31261 caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa
    31321 ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga
    31381 ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc
    31441 acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct
    31501 gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac
    31561 tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg
    31621 caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta
    31681 ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag
    31741 atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg
    31801 acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat
    31861 cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat
    31921 tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc
    31981 accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc
    32041 gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat
    32101 ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat
    32161 cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg
    32221 gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa
    32281 aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg
    32341 tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag
    32401 tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg
    32461 cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact
    32521 tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa
    32581 tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca
    32641 gttgggttat tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag
    32701 attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat
    32761 atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat
    32821 ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct
    32881 ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc
    32941 gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac
    33001 agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc
    33061 acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg
    33121 gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg
    33181 attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat
    33241 ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg
    33301 atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc
    33361 tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag
    33421 ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac
    33481 atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc
    33541 gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt
    33601 tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg
    33661 ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc
    33721 tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt
    33781 ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt
    33841 atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc
    33901 ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc
    33961 tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc
    34021 tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca
    34081 gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg
    34141 aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct
    34201 aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac
    34261 gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat
    34321 ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag
    34381 ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa
    34441 tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat
    34501 aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat
    34561 acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg
    34621 acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc
    34681 ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa
    34741 cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca
    34801 gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg
    34861 gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc
    34921 tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct
    34981 aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa
    35041 aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat
    35101 tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat
    35161 atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca
    35221 aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa
    35281 ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg
    35341 actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc
    35401 gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt
    35461 ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca
    35521 gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg
    35581 attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat
    35641 gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa
    35701 tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta
    35761 cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac
    35821 gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg
    35881 aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac
    35941 gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg
    36001 aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa
    36061 tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta
    36121 aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg
    36181 actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact
    36241 acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat
    36301 tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt
    36361 tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa
    36421 caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa
    36481 tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag
    36541 caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc
    36601 taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg
    36661 gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg
    36721 atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag
    36781 aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca
    36841 ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa
    36901 tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg
    36961 atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag
    37021 aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc
    37081 cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg
    37141 gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac
    37201 tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag
    37261 ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa
    37321 tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag
    37381 cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga
    37441 atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat
    37501 acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata
    37561 taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga
    37621 ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt
    37681 atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt
    37741 tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata
    37801 tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta
    37861 tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg
    37921 atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt
    37981 tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa
    38041 caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg
    38101 gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa
    38161 aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac
    38221 atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca
    38281 atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac
    38341 gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa
    38401 gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt
    38461 ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc
    38521 taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga
    38581 aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg
    38641 acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat
    38701 gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact
    38761 gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca
    38821 actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg
    38881 atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt
    38941 tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg
    39001 gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc
    39061 agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga
    39121 tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat
    39181 ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc
    39241 ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca
    39301 ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg
    39361 ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa
    39421 caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc
    39481 ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt
    39541 ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg
    39601 ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag
    39661 tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg
    39721 catactttag tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg
    39781 gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc
    39841 agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca
    39901 attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa
    39961 ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa
    40021 aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa
    40081 gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct
    40141 gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa
    40201 ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact
    40261 gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg
    40321 gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg
    40381 caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg
    40441 attgattggt acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat
    40501 ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga
    40561 agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca
    40621 ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat
    40681 atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg
    40741 gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct
    40801 gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca
    40861 ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga
    40921 tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc
    40981 tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc
    41041 taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg
    41101 gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc
    41161 taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact
    41221 tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg
    41281 tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac
    41341 taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac
    41401 agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg
    41461 aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac
    41521 tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg
    41581 agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat
    41641 atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa
    41701 tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc
    41761 aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt
    41821 atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct
    41881 atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta
    41941 cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg
    42001 caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt
    42061 ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga
    42121 aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta
    42181 aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc
    42241 aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg
    42301 aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac
    42361 gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg
    42421 aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc
    42481 tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt
    42541 gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa
    42601 ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg attggcatat
    42661 cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat
    42721 actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga
    42781 taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa
    42841 tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact
    42901 ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga
    42961 agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc
    43021 aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc
    43081 caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag
    43141 ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg
    43201 gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac taggacattt
    43261 gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt
    43321 ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct
    43381 ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt
    43441 caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat
    43501 ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc
    43561 tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa
    43621 agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga
    43681 agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga
    43741 aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat
    43801 caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa
    43861 gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac
    43921 ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga
    43981 aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc
    44041 agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac
    44101 gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc
    44161 agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt
    44221 actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt
    44281 tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa
    44341 agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa
    44401 aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca
    44461 atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac
    44521 ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa
    44581 aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa
    44641 agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt
    44701 cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag
    44761 tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa
    44821 tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa
    44881 caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca
    44941 attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc
    45001 tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca
    45061 agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga
    45121 aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc
    45181 aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca
    45241 gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg
    45301 ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca
    45361 gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt
    45421 agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca
    45481 attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga
    45541 acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac
    45601 aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgcaag
    45661 aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta
    45721 caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag
    45781 tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga tacgacgact
    45841 tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca
    45901 attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga
    45961 aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca
    46021 gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa
    46081 agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct
    46141 gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca
    46201 acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca
    46261 agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag
    46321 ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga
    46381 aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt
    46441 acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc
    46501 ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca
    46561 ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact
    46621 ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga
    46681 tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat
    46741 ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag
    46801 agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag
    46861 caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc
    46921 agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt
    46981 ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt
    47041 tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca
    47101 taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg
    47161 tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat
    47221 cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt
    47281 gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag agctttctat
    47341 tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa
    47401 aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca
    47461 gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca
    47521 tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg
    47581 aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca
    47641 gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca
    47701 attttccgat caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt
    47761 gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca
    47821 agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt
    47881 gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt
    47941 taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga
    48001 agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa
    48061 aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca
    48121 tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt
    48181 acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt
    48241 gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt
    48301 tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca
    48361 accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg
    48421 gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta
    48481 tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca
    48541 agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa
    48601 aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc
    48661 gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa
    48721 aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt
    48781 aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca
    48841 gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt
    48901 agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt
    48961 aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac
    49021 agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca
    49081 aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac
    49141 tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat
    49201 cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc
    49261 gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt
    49321 tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa
    49381 tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat
    49441 gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact
    49501 tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta
    49561 ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa
    49621 caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga
    49681 gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg
    49741 gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa
    49801 ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg
    49861 gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa
    49921 tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact
    49981 ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac
    50041 gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga
    50101 gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt
    50161 tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat
    50221 ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga
    50281 tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg
    50341 ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta
    50401 tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa
    50461 atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga
    50521 atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt
    50581 gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc
    50641 tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct
    50701 tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc
    50761 atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg
    50821 cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg
    50881 accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact
    50941 gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac
    51001 tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt
    51061 tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa
    51121 acacgtcaat aagtttgatg aacaaattaa tcattatggt agaagtcgat tgatttattt
    51181 taatgcaatc tctgtggaat tttcgaaaga tttatttttt taatacagtc tcaatataac
    51241 tattcaaaca taaaataacg ttatttgatt tatttgttta tagtaaattt tatttagtaa
    51301 tcattgcttt aaaatactac gcttatataa cagagaaaat aagcgttata ataaagaaca
    51361 gtattattta aaatgacaga aaacattact attaaagaaa taaaaaacat aaaaaatctt
    51421 aagaaatcgg tttcatttag cttttgagat aaaataatag tacagtttta tacgctgttt
    51481 catatgttta gaaaatttga attagatttg aaaactctaa tttttaaatg ataaaaaaag
    51541 cgagaatgct ggttttactg tatattaacc ttagataact ttcatttttt taaaaaaatt
    51601 atgaaaaaaa ggtagctatt taaaagttga ttatgtataa tagaaacaga tgggagatta
    51661 ttatgatctt ctgttacaaa aaagtgattc ttatcacgaa aaggagtggt gcacgttgtt
    51721 aacattatat acttccccaa gttgtacttc ctgcagaaag gctcgtgcgt ggttgcaaga
    51781 acatcagatt ccatttgttg aacgtaatat tttttcagaa ccattgaaca gttctgaatt
    51841 aaaagcgatt ttacaaatga cagaagacgg aacagaagaa atcatctcga cacgttccaa
    51901 agtattccaa aaactgaaca tggatttaga tgatctgccg ttacaagaat tattggaact
    51961 tgtgcaaaac aatcctggat tgcttcgccg tccaatcatg attgatgata aacgtctgca
    52021 agttggtttt aatgaagatg aaattcgtcg gttcctaccg cgcgatgtac gacaattaga
    52081 attacgtcaa gcacaattga tggctggatt atagaaggat aatacgaaac ttggaaaaga
    52141 c
//
LOCUS       DACEQH010000015        50795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.015, whole genome shotgun sequence.
ACCESSION   DACEQH010000015 DACEQH010000000
VERSION     DACEQH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.015"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..100)
                     /locus_tag="IW833_000898"
     CDS             complement(<1..100)
                     /locus_tag="IW833_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAQ1307033.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVF"
     gene            complement(115..1362)
                     /locus_tag="IW833_000899"
     CDS             complement(115..1362)
                     /locus_tag="IW833_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1307034.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(1438..1584)
                     /locus_tag="IW833_000900"
     CDS             complement(1438..1584)
                     /locus_tag="IW833_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ1307035.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(1688..1999)
                     /locus_tag="IW833_000901"
     CDS             complement(1688..1999)
                     /locus_tag="IW833_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1307036.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(2001..2198)
                     /locus_tag="IW833_000902"
     CDS             complement(2001..2198)
                     /locus_tag="IW833_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ1307037.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(2592..4319)
                     /locus_tag="IW833_000903"
     CDS             complement(2592..4319)
                     /locus_tag="IW833_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1307038.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(4312..5505)
                     /locus_tag="IW833_000904"
     CDS             complement(4312..5505)
                     /locus_tag="IW833_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1307039.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(5692..6336)
                     /locus_tag="IW833_000905"
     CDS             complement(5692..6336)
                     /locus_tag="IW833_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1307040.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(6430..6564)
                     /locus_tag="IW833_000906"
     CDS             complement(6430..6564)
                     /locus_tag="IW833_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ1307041.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(6566..8698)
                     /gene="feoB"
                     /locus_tag="IW833_000907"
     CDS             complement(6566..8698)
                     /gene="feoB"
                     /locus_tag="IW833_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ1307042.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(8695..9168)
                     /locus_tag="IW833_000908"
     CDS             complement(8695..9168)
                     /locus_tag="IW833_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ1307043.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            9569..9793
                     /gene="nrdH"
                     /locus_tag="IW833_000909"
     CDS             9569..9793
                     /gene="nrdH"
                     /locus_tag="IW833_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ1307044.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            9952..12111
                     /gene="nrdE"
                     /locus_tag="IW833_000910"
     CDS             9952..12111
                     /gene="nrdE"
                     /locus_tag="IW833_000910"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ1307045.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            12161..13126
                     /gene="nrdF"
                     /locus_tag="IW833_000911"
     CDS             12161..13126
                     /gene="nrdF"
                     /locus_tag="IW833_000911"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ1307046.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(13215..14354)
                     /locus_tag="IW833_000912"
     CDS             complement(13215..14354)
                     /locus_tag="IW833_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1307047.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(14663..15376)
                     /locus_tag="IW833_000913"
     CDS             complement(14663..15376)
                     /locus_tag="IW833_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ1307048.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(15630..16331)
                     /gene="nagB"
                     /locus_tag="IW833_000914"
     CDS             complement(15630..16331)
                     /gene="nagB"
                     /locus_tag="IW833_000914"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ1307049.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            16565..18097
                     /locus_tag="IW833_000915"
     CDS             16565..18097
                     /locus_tag="IW833_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1307050.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQINWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(18288..19490)
                     /locus_tag="IW833_000916"
     CDS             complement(18288..19490)
                     /locus_tag="IW833_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1307051.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(19572..20261)
                     /locus_tag="IW833_000917"
     CDS             complement(19572..20261)
                     /locus_tag="IW833_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1307052.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(20367..20735)
                     /gene="rplL"
                     /locus_tag="IW833_000918"
     CDS             complement(20367..20735)
                     /gene="rplL"
                     /locus_tag="IW833_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ1307053.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(20788..21291)
                     /locus_tag="IW833_000919"
     CDS             complement(20788..21291)
                     /locus_tag="IW833_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ1307054.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(21701..22390)
                     /gene="rplA"
                     /locus_tag="IW833_000920"
     CDS             complement(21701..22390)
                     /gene="rplA"
                     /locus_tag="IW833_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ1307055.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(22494..22916)
                     /gene="rplK"
                     /locus_tag="IW833_000921"
     CDS             complement(22494..22916)
                     /gene="rplK"
                     /locus_tag="IW833_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ1307056.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            23259..23927
                     /gene="sdaAB"
                     /locus_tag="IW833_000922"
     CDS             23259..23927
                     /gene="sdaAB"
                     /locus_tag="IW833_000922"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ1307057.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            23940..24812
                     /gene="sdaAA"
                     /locus_tag="IW833_000923"
     CDS             23940..24812
                     /gene="sdaAA"
                     /locus_tag="IW833_000923"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ1307058.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(24903..25922)
                     /locus_tag="IW833_000924"
     CDS             complement(24903..25922)
                     /locus_tag="IW833_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1307059.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(26095..26982)
                     /locus_tag="IW833_000925"
     CDS             complement(26095..26982)
                     /locus_tag="IW833_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1307060.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSRVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(27059..27604)
                     /gene="nusG"
                     /locus_tag="IW833_000926"
     CDS             complement(27059..27604)
                     /gene="nusG"
                     /locus_tag="IW833_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ1307061.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(27705..27875)
                     /gene="secE"
                     /locus_tag="IW833_000927"
     CDS             complement(27705..27875)
                     /gene="secE"
                     /locus_tag="IW833_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ1307062.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(27899..28051)
                     /gene="rpmG"
                     /locus_tag="IW833_000928"
     CDS             complement(27899..28051)
                     /gene="rpmG"
                     /locus_tag="IW833_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1307063.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            28213..28857
                     /locus_tag="IW833_000929"
     CDS             28213..28857
                     /locus_tag="IW833_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1307064.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(28913..29416)
                     /locus_tag="IW833_000930"
     CDS             complement(28913..29416)
                     /locus_tag="IW833_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1307065.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(29429..29475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            30013..30978
                     /locus_tag="IW833_000931"
     CDS             30013..30978
                     /locus_tag="IW833_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1307066.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(31077..32249)
                     /locus_tag="IW833_000932"
     CDS             complement(31077..32249)
                     /locus_tag="IW833_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1307067.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAVFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(32236..33381)
                     /locus_tag="IW833_000933"
     CDS             complement(32236..33381)
                     /locus_tag="IW833_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1307068.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(33395..34319)
                     /locus_tag="IW833_000934"
                     /pseudo
     CDS             complement(33395..34319)
                     /locus_tag="IW833_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            34436..35515
                     /locus_tag="IW833_000935"
     CDS             34436..35515
                     /locus_tag="IW833_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1307069.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            35547..36140
                     /locus_tag="IW833_000936"
     CDS             35547..36140
                     /locus_tag="IW833_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1307070.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(36250..36477)
                     /locus_tag="IW833_000937"
     CDS             complement(36250..36477)
                     /locus_tag="IW833_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1307071.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(36580..39168)
                     /locus_tag="IW833_000938"
     CDS             complement(36580..39168)
                     /locus_tag="IW833_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ1307072.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(39188..39847)
                     /locus_tag="IW833_000939"
     CDS             complement(39188..39847)
                     /locus_tag="IW833_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1307073.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(39896..40399)
                     /gene="trmL"
                     /locus_tag="IW833_000940"
     CDS             complement(39896..40399)
                     /gene="trmL"
                     /locus_tag="IW833_000940"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ1307074.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(40620..41486)
                     /locus_tag="IW833_000941"
     CDS             complement(40620..41486)
                     /locus_tag="IW833_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1307075.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            41595..42236
                     /locus_tag="IW833_000942"
     CDS             41595..42236
                     /locus_tag="IW833_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ1307076.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(42298..43659)
                     /gene="mgtE"
                     /locus_tag="IW833_000943"
     CDS             complement(42298..43659)
                     /gene="mgtE"
                     /locus_tag="IW833_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ1307077.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(43677..44576)
                     /locus_tag="IW833_000944"
     CDS             complement(43677..44576)
                     /locus_tag="IW833_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1307078.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(44578..45375)
                     /locus_tag="IW833_000945"
     CDS             complement(44578..45375)
                     /locus_tag="IW833_000945"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ1307079.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(45353..46057)
                     /locus_tag="IW833_000946"
     CDS             complement(45353..46057)
                     /locus_tag="IW833_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ1307080.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            46188..46763
                     /locus_tag="IW833_000947"
     CDS             46188..46763
                     /locus_tag="IW833_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ1307081.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            46881..47558
                     /locus_tag="IW833_000948"
     CDS             46881..47558
                     /locus_tag="IW833_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1307082.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(47637..49445)
                     /gene="pepF"
                     /locus_tag="IW833_000949"
     CDS             complement(47637..49445)
                     /gene="pepF"
                     /locus_tag="IW833_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ1307083.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(49621..50619)
                     /locus_tag="IW833_000950"
     CDS             complement(49621..50619)
                     /locus_tag="IW833_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ1307084.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
BASE COUNT        15310 a        10558 c         8829 g        16098 t
ORIGIN      
        1 taaatacttt atcgtgaaaa agaatgaaat tatcaataat tcgttctaac tgatttaatt
       61 gtataatttg atcttcacat aatattattg gataactcat aaatcctcct tccctcaaaa
      121 caacaaaata acttgagtag taaattgata cggatcgttt tgatagttca caaacatatt
      181 gggattcttt ttattgattt cctctatact agataaacct aacccttgat gtcccttttt
      241 tgttgttatt ccttttttca ttagagtatc tatagagagt agtatctctc tacaactatt
      301 cgatatcgaa atttctatct gtttatcatc tcggtaaata gctagagaca gttctctatc
      361 ttgactctct atagatgctt caattgcatt atcgagaata attccaagca ccctaataca
      421 gtcaaatatt ggaacaggaa cttcaataat agtacttgga cattcgaatt tacaacaaat
      481 cttattgtta tttgcttggt atagcttcgc tatcaacaaa cttttcaaat attcattttt
      541 tatatttttt aaataacgat attcaaactt attggttaga taagaattag aatagatctc
      601 taattctttt atttcttttt ccaatatcga atcatggttt tgattagcga tttcttttaa
      661 actcagcaat aaatttttat agtcatgtct aaatctggca aatttttctt gatctttttc
      721 cagtgaatca gtatattttt ttagataaac caattctttt cttttcaatt gctgttcata
      781 ttgttctcgt tgcctaatag ttattctaat aaataaaaat agaacaacaa acatttgaat
      841 gattaaaaat attaagacac caaacacaaa atgatcaaat acttgatatt gatgagcaac
      901 ataagaaaca aataaaccaa ttgtaagtaa ataaatcatg aatatagcag taagtttaga
      961 actggactgt tcaacaatcc tatttatagc taattttcta tataaccaaa taaacatcag
     1021 caaaaacaaa gaatcaagca ttatctggaa tactacataa ccataccctt ttatcccttc
     1081 aaatgaaaac atatacgtca taaccatgga agtaataata gatctgaaat catcaataag
     1141 catacataat agtaatttat ttaaaagcac tatattagtt ttcttctttt cctttaatat
     1201 aaaataactt ccgattatag gtatagctgc ggaaacatca gtaaataatt caacaaaaaa
     1261 agtgagtggt atcaataaac atacaaaaag atattttctt ttgttaagat cagtattagt
     1321 catataaaat aagagaaaaa agaaaaagtt tcccacgaac attcttaata gataagcttt
     1381 tgtatcgata gtaatcaaga gatcccctcc tgattttagg atatcataaa aaaaatttta
     1441 gttacacttt ttaaataatg aaaaaacaca ttccactaaa tttgatgccg gttttccttg
     1501 aggttttgtt ccagcacctc ctttaatttt tttaagctct tgatctgaac attgtttagc
     1561 atttagtcta tttttttctt ccattacgat tccccctaca aactataaat acttttatgt
     1621 cactgattca atatacctat ttttctaatc aaaaaatttt gagacatatt taatattata
     1681 agtatcatta aaattgagat ttatctccat aatctgctcg atataaacca tttattccag
     1741 cagttcttcc aatttcactc aaatttctta ttaattcctc ttgatattca gtgaatctta
     1801 ttttattagt ataagcagta aaatatagat aattcaccaa tctatttatt aaaggaccgg
     1861 gatctaattc atatttttgt ttttctaatt ttaaatatac cttttgtaaa acctcaagaa
     1921 tatcagaata tttaggatct ttacttatag atatatcatt atataattca tgaacaattt
     1981 gcttagcatt ttttttcatt ttagcacttc cctggaattg ctccacctaa aaaaccacct
     2041 atagacattc ctgcaataca agtagttgcc ttggcccaat cgaccgtaca tttattttta
     2101 gtgcaataca ctccatttcc ataatatttt ccactatgag tggtaccccc atagataagt
     2161 tgtgtctctt taatagacaa aatttttaaa tgtttcataa tctaatcacc cctaaattaa
     2221 tgtttttaga ctattattct aacattcatt aaatttcaaa aaacaattcc ttcttgaaac
     2281 gtagcaaaat aattcctgaa taagaattta atcactttta agtatataaa ctatgtatat
     2341 tctttaatat tttttgagaa tgcacttcgt acataataaa ctttaatcac attagtcttt
     2401 catacctacg atcgatctct ttgactcgca cccataaatt cataatagtt tgtcggcgtc
     2461 tttggacgtt tcgaagatga gctacgcatt ctcttttttt atagacaaaa aagatagaga
     2521 cgaacatgga gcttccaaat ttggatattt gatccaattt tggaagctcc atttatttcg
     2581 tgtctgtctt tttatttttt ccagctttcg actaattctt ggttctcttt gatccagtct
     2641 tttgctgctt gctgtggatc ttttccattg ttgatttcca acataacttt ttccatatct
     2701 ttttctgacc aatggaaatt gtctaatact ttgtaagctt ctggctggtc ttctttcaat
     2761 ccttttcgaa ccatcgtatg gatctgctct tcgcttccca ttccgttttc aggatcttct
     2821 aagtatttca gatcatattt ggcaaacatc caatgaggtg tccatcctgt aactacaatt
     2881 ggttcgtgtt ttttgatcgc ttgacctagt gcaacggtca ttgctcctga agaagatgtc
     2941 tctactttcc agtctttcaa gttgtcatat ttttggatcg tgttttcagc agccgtaact
     3001 acaccagctc ctggctcgat tcctatgatt ttcttacctg cctgatcggt taactcatcg
     3061 atcgaattta catccatgta ggctggcact gctaaaccaa cttttgctcc tgtcaaattg
     3121 gcacctaggt cttcgacttg atctttatat tgtgcatatt gtgaaccatg agttttaggt
     3181 agccaagctg aaaccatggc atctgactcg ccatttgaaa cagatttcca catgattgaa
     3241 ttatctaatg gcgtcatcgt tacatcatag cccatttgtt tcaatacttc accgactaca
     3301 tttgttgaag caacttctgt atcccattca acataagata agttgatacg ttttgtttct
     3361 ttggcagatg aaaaactccc tataccaatc gcacctaaga tcactactac tacagcacca
     3421 atgatcagtc cttgacgttt tttacttttc tgtgttgttg ctcctgcagc ttttttattg
     3481 tttggctggt tcaaatgctg tgtaaaacgg tcaatgatga ttgctaagat aactaatgca
     3541 accccgttga caaatccatt cccgacttgt gctctttgca atgctgataa tacgccgcgt
     3601 cctaagccgg gtgcaccgat catagaggca ataacgacca ttgaaagtga cagcattgtt
     3661 gtttgattga tccctgccat gatcgtactt ttagctaaag gcaattctag tttgaatagt
     3721 ttttgacgac ttgtgctccc gaatgaatca gaagcttcta ctaattcttt aggaacttgt
     3781 cgaatcccta aattcgtaaa acgaacggtt ggtggcagag caaagatgac tgaggcaaat
     3841 acccctggaa ccatcccgat accgaaaaat gcaactgctg gaatcaaata aacgaatcct
     3901 ggcattgtct gcataaagtc aaggatcggt ttgataatac tttgggcttt actgcttttt
     3961 gccatcaaga tccctaatgg taccccaatg atgatcgaga tcacactaga taaaagaaca
     4021 agtgtgacag tattcatcaa atcattccat agaccttggt tataaataaa ccatagaccg
     4081 attaatgtga acaacgtaag tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc
     4141 aataacaaga taaacaaagg agctggaatc actaacagta aatttgtaat ccccgacatc
     4201 actgcctgtc caatcgtttg taaaaatgaa aataggccag aaaaagttgt cgtaaaccat
     4261 tctgtgatat tttctaccca gtttgccact ggtaattgat agttcaatag attattcatg
     4321 ttcactcacc tcgcttgttt ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc
     4381 ttttaatttt ccatcctcta caactgcaag tggagtcggt gagtcataaa taatattgaa
     4441 aatatcactt accaccatgt ctttcccaat caccgtcaca ttttcatcta ggaagtcgat
     4501 caatggacgt ttttgcttcc tagcttctaa cgcttgatct gcagtgatga tccctttcag
     4561 gtgtctcttt ctgtcaacag ccatcagcat gctgacttct tcattacgca tccttgtcaa
     4621 tgcaacgttt ggtccatcag attcgatatt cgtcgtcaat gcaggtacca tgatgttttg
     4681 agccgtaagc actttggaac gatcgacttc ttccacaaat tcacgtacat aatcatttgc
     4741 tggattggtc aatatttctt cacctgttcc gatctgcatg atttcgccgt ctttcatcaa
     4801 ggcgattctg tccccaatcc gtaaagcttc attcaaatca tgggtaatga aaataatcgt
     4861 tttttgaaca ttggcttgca ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg
     4921 atcaagagcc gagaacgctt cgtccatcag caagatctca ggatcatttg ctaaagctct
     4981 ggctaggcca acccgttgtt gcatcccacc agataactga ctaggatatt ggtctttaaa
     5041 tgaaagcaaa ctagaatttt ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg
     5101 tacaccacgg acttctaacc cgtattctgt attttccaag atcgttcgat gtggaaacag
     5161 accgaagttt tggaaaacca tattgatttt atgacgacgg acttctcgca attcttcttt
     5221 gcttaacttc gatacatctt ctccatcaat atagatactg cccgaagttg gctcaatcag
     5281 acgattcaat agacggatca atgtggattt cccactcccg gaaagcccca tgatcacgaa
     5341 gatttctcct tcttttacgt caaaattcac gtcatacaca ccaaccgttg caccggtttt
     5401 ctttaaaatc tccgttttcg gctggtgatc attcatcata tctaaagcgg cttgtgtctt
     5461 tttaccaaat atctttgtta aatgttttac ttctactttt accaaacaaa ttctcctttt
     5521 ttatgtaaat tcatttttct ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt
     5581 cactttgtca aaaatgaaag aaatttgctt gtccaaactg aaaaaaactt ggctaaatct
     5641 ttgtaaataa aaggtttagc caagttaata tttttttaat atttcacaca atcattctgc
     5701 aagctgatgc tccgggtaaa aaaagtttgt catacgctgt agtgtatatt catttccaat
     5761 aaaatgaacg atgtcccctg ccgtaaattt agcatagggg ccaggtgaaa caagcagttc
     5821 ttcattatga gaaattgcga taactgtggc gccagtcgcc tgccagatat tcaactcgct
     5881 aattgaacgg tccagatgca ttgctttttc tgataaagat aattcaaacg gaagtaaagg
     5941 attcatctta tgtattcgct tcgtttgatt gaccagctga tccaataatt gagaaaaatg
     6001 attcagctca tcttgttgct tctccacact tgccatgatt tctgcttttg tatcttggat
     6061 ggattgtaca tcttgatatt gcaataggaa atcttttgct ttttccttcg aagcaacaaa
     6121 tgccccactt ccatgccgta cttccatgat tccaagatct accagcacat tgatagcctt
     6181 gcgggtagtc tcaggagaag cattgtacgt actggcaagt gttgagcggg catggatctt
     6241 ctcacccact ttatatcggt tttcaacgat acgttcggct aaatcaacag caatctgctg
     6301 gtagcgcggg cggacgattt ttttctttcc agacattttc tttcctcttt ctttttttac
     6361 tttccctcat cttactatgc tctagcaaaa taagcaaaaa aggggagaat agcctcttgc
     6421 aacgactgat catcgcttga tctgttcctt ttttactggg caagctgttt gacagtcatc
     6481 acaattctta ccggatttta aacgagtata gacaattgcg cctgcacttc caaagattaa
     6541 cacacttaat aagaatgtcg ccatcctatt cctctccttc taatgacaat atagtataaa
     6601 actggttctt ggattttggc tgacggaaca aaacataaac gagtcctatc atcagtaaaa
     6661 tagcaagata cgtccctaac gctacctgtc caccctccaa taagtgtccc aactgataaa
     6721 tcacaaaact aactaaataa gctaaaccgc attgatagcc tatggcgatc caagtccatt
     6781 tcttggcatt catttcccga tggatagccc caattgccgc aaaacaaggc gcacaaagta
     6841 aattgaaggc taacaatgaa taagctgctg cgggagtata tgtgtgttgt agcacttgcc
     6901 agatttctct gccattttcg gatacttccg catgaccaaa caaaatacca aatgtgccaa
     6961 taatattttc tttagcgacc aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc
     7021 aaccaagcgg ctgaaaaaca actgccagga ttcgcccgaa aaaggctaag atactttggt
     7081 cttcagaaac aggttgagca taaaaattat aagaagagct gaaccagatc acgatactag
     7141 aaacaaagat aatcgttcct gcttttttaa caaaagactt gctgcggtca taggtttgtc
     7201 ttaggaccgc tgaccattga ggaaaatgat atgcgggcaa ttccatgata aaaggagctg
     7261 tatctccagc aaacaaacgt gtttttttca agctgattcc agagaaaata attgcagtca
     7321 taccaagaaa ataggcagaa ggagctaccc aagattgatg aggaaaaaag gcccctgcta
     7381 ctaaggcaat aatcggcaac tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca
     7441 aacgacgatc ttgctcgttt tcaatcgtgc gactggccat gacgcctggc actccacatc
     7501 ctgtagcaat cagcatagga atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc
     7561 gatccatgac aaaagcgatc ctcgccatat atccgcagtc ttccagaaag ctcaagcata
     7621 aaaacaatat aatcaattgc ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc
     7681 cgttcaagat cagctcctgc atccatcccg ctacttgcca ttcagctaac aatctgccaa
     7741 catgttctgg taaccatgta ccaaacaatg tgtcattgat ccaatctgtc cccattgttc
     7801 ctactgtttg aatagccaga taatagatca gccacatcac aaatgcgaag atcggcaagg
     7861 ctaaccaacg attcgttgca atctgatcga ttttgtcact catcgataat tggaaagaat
     7921 catttgacac aacacagagt gtacatagtt gggtaataaa ttcataacgt tcattgacca
     7981 gaatcgtctc actatcgtcg tccaatagtt tctctgttaa ctggatcagt tgctcgatct
     8041 ctttttgctg agaaactgct atctcgtaat cctctcttac cttctgatct cgttcaaaat
     8101 atttcattgc ataccagcgt ttcttgccgc tttgttctcc tagtacatct tcaatttcat
     8161 gtaaagctgc ttctaaacgt ggatcatatg tacagggaac aggaatagaa ggccgctttt
     8221 ttgcagactc ctgaatcttt tcgattagtt tttcgattcc ttgctttttc aatacgctga
     8281 ttggtaaaac tggtacacca agaccatagg aaagcttttc tagattgatt ttttttcctt
     8341 cttttttgat gacatccatc atattcagac caatgatcat aggaatgtct aaatccaata
     8401 actgcgtagt taaataaaga tttcgttcta aattggtagc atcaataatg ttgatgatca
     8461 gttcaggctg ttcctgaacc aaataatctc ttgcaatcac ttcttctggt gtataaggag
     8521 acaaagaata gatgccgggt aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc
     8581 cttctttttt ttctactgtt acgcccggcc aattccctac atattgattc gaaccagtca
     8641 agagattgaa caagctagtt ttcccgctgt ttggatttcc gactaacgcg atattcattc
     8701 tttttcctcc ctttgtatca agattagttc ggcctctgac tttcgtaacg tcagttcata
     8761 ccctcgcact gtcagttcga ttggatctcc cagaggggct agttttcgga taactatttt
     8821 tacatttcgc gtcaatccca tatccattaa ccgtcgcttc actgcgcctt gtccgtggat
     8881 tgctacgacg atcccagtct ctcctgcttt taattgatca agagaagtcc agctttcaga
     8941 ggaagcagtc tttttattga caaagatttg acctagtatt tcccgactta ctgccactcg
     9001 atgacttttt accagtaaaa tggcattgtt gcctgataaa ttgacgacga cgattttact
     9061 tccggcaacg atcccgatat tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg
     9121 ttcgacttga taaacgaaat tctgctttgc ctgcgctaag gtctgcatat tgattcctct
     9181 cttgtgaaat aaatcatgta ttaaaatcat tcattatatg ttctaatttt cttacgtgtt
     9241 acgcaaataa atgagaatca ttctcacata gaatactctc ggtaattgag aatgtcaatt
     9301 atacttgtga tattatgagc gaatctttag tattgataga taaaaatgag aaaaaaatat
     9361 ttcttcatag ttttccacaa ttttttctct attaaatttg ctattttccc acacgatact
     9421 tgttttcagc tcttttttca aaaacacaaa acttagtatt ccacagtttc cacaacacaa
     9481 tatgtagtgt ttgttgtttg acttttttac taagagtttt atactatcta ttatagataa
     9541 taagaatgtg ttagaggagg cactcaccat gaacgtaaaa atcttttcta aaaataattg
     9601 tatgcaatgt aagatggtca aacggttttt aacagaaaat caaattgctt ttgaagaagt
     9661 caatatcgat gaacagcctg aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt
     9721 tcctgtcatt acttctgatg cagaaacagt tatcggattc cgtcctgatc agttgcgtaa
     9781 acttgccagc taacatgact aaaagaatca aaacgtgata catggatttg aagggcttga
     9841 gacagcacca gtttttgtcg caaacttcca aatccttttt taaagcttaa aaacaatgca
     9901 atgatttgca atcaagcatc atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt
     9961 aaaaatttac aagatgtcag ttatttcaag ctgaataatg aaatcaatcg gccagtcaat
    10021 ggtcaaatcc ctttgaataa ggataaagaa gctttagttg ccttttttga ggaaaatgtc
    10081 aaaccaaata cgatgacttt tctatctatt atggaaaaaa tcaattactt gatcgatcaa
    10141 gaatatatag aagcagaatt cattcaattg tattcaccat catttattga aaagctatat
    10201 caatttttgg acgagcaaca tttttctttc aaatctttta tggctgctta caaattttat
    10261 tctcaatatg cgttgaaaac caacgacggt tccgcttatc tggaaactta tgaagatcga
    10321 gtagccttca atgcgcttta ctttgcgaac ggggatgaag aattagcact tgctcttgca
    10381 gacgaaatga tccatcaacg atatcagcca gctacacctt ctttcttgaa tgctggacgt
    10441 aaacgtcggg gcgaattggt gtcttgtttc ttgactcaag tcactgacga tatgaactct
    10501 atcgggcgct caatcaactc tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt
    10561 actttatcaa atcttcgtga agccggcgct cctatcaaag gatatgaggg tgctgcaagt
    10621 ggtgtcgttc cagtcatgaa attattcgaa gatagtttca gctattctaa tcaattaggt
    10681 cagcgtcaag gagctggcgt ggtttatttg aacgttttcc atccagacat tattgctttc
    10741 ctttcagcaa aaaaagaaaa tgcagatgaa aagattcgcg taaaaactct ttctcttggc
    10801 gtgatcgtac ctgataaatt ttatgaattg acacgtaaaa acgaagatat gtatttattt
    10861 agtccatata gtgtcgaaaa agaatatggt gtcccattct cttatgtaaa tattacagct
    10921 gagtatgatc atctagttgc aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat
    10981 cttgaaaatg aaatctcaaa attacaacaa gaatctggct atccttacgt aatcaatatt
    11041 gatacagcga accgcgagaa tccaatcgat ggcaagatca tcatgagtaa tttatgttca
    11101 gagattttac aagttcaaac gccttctgtg attaacggca aacaggaata tgaagtatta
    11161 ggaacggata tctcatgtaa cttaggctcg acaaatatcg tgaatttgat gcaaagccct
    11221 aactttggtc aatctgtgcg tacgatgact cgtgcattga catttattac agatgcatct
    11281 gatatcgatg tcgtaccacc gatccaaaac ggaaataagc tgaatcatac gattggatta
    11341 ggtgccatgg gcttacacac ttatcttgca aaagaacaaa tggaatatgg ctcagaggat
    11401 tcattagact ttacaaatat ttacttcatg ctgttgaatt attggacact tgttgaaagc
    11461 aacaatatcg ctcgtgaaag aaaacaagtt ttccataatt ttgaaaaatc agcttatgct
    11521 gacggcactt atttcgaaaa atacgtaact ggagaattcc agccaaaatc agaaaaagtc
    11581 aaagcattgt ttgaaggcat ttttatccca actgctgaag attggaatgc tttgaaacaa
    11641 gcagtaatga aagatggact gtatcatcaa aatcgcttgg cagtagcacc aaatggctca
    11701 atttcatata tcaacgatac gagtgcaagt attcatccta tcactcgcat gatcgaagaa
    11761 cgccaagaga agaaaatcgg taaaatttat tacccagcgc cttatctatc caatgagacg
    11821 attccgtatt acacttctgc ttatgatatg gacatgcgta aagtaatcga tgtttatgca
    11881 atggcacaac aacacgtcga tcaaggcatg agtttgactt tattcatgcg ttctgaaatc
    11941 ccagaaggca tttatgaatg gaagacaaca acgaagcaaa cgacacgtga tttgaatatt
    12001 ttaagacatt acgctttcca caaaggaatc aaatccattt attacgtacg tacctttact
    12061 gatgatgcag aagaaatcgg aagcaatcaa tgtgaaagct gcgtgatcta atatcataga
    12121 tagattcgct tattcatatt ttaattaaag gagcattctc atgtcagaaa catattatga
    12181 agcaataaac tggaatgaaa ttgaagatat cattgataaa tccacttggg agaagctgac
    12241 agagcaattt tggctagata cacgtatccc actttcaaat gacctagatg attggcgtac
    12301 attgtctcaa ttggaaaaag atacagtagg acatgtattt ggcggtttga cactgctaga
    12361 tactgtccaa tctgaaagtg gcatggatca gttgcgtaaa gatgtccgca ctccccatga
    12421 agaagccgtt ttgaacaata ttcaattcat ggaatccgtt catgccaaaa gttattcttc
    12481 tatttttagt acactgaata cgaaaaaaga aatcgaagaa atttttgact ggacaaatac
    12541 gaatccttat ctgcaaaaaa aagctgagcg tatcaatgaa atctacaaac acggtacacc
    12601 gctagaaaag aaaattgcca gcgtcttttt agaaacattc ttgttctatt caggttttta
    12661 tactcctctt tattatttag gaaacaacaa attggctaac gtagcagaga tcatcaaact
    12721 aatcatccga gatgaatctg tacatggaac ctacatcggt tacaaattcc aattaggatt
    12781 caacgagtta cctgaagcag aacaagaaac gttaaaagac tggatgtaca atcttcttta
    12841 tgagctttac gaaaacgaag aacgttatac agaagaatta tacgatccaa tcggctggac
    12901 agaagaagtc aagacattcc tacgctataa tgccaacaaa gcattgatga acctagggat
    12961 ggatccactc tttccagata ctgccaatga tgtcaaccca atcgtaatga acgggatttc
    13021 aacaggcaca agcaaccatg acttcttctc acaagtcgga aacggctatt tgttaggtca
    13081 tgtagaagcg atgaaagacg acgattattt gattggattg gaataaggat ttattttaat
    13141 tcaaaaattt ttcgaaaaat aaaaagagtt tagcgtacag atattttaaa tgtacactga
    13201 actctttttt atgcttattc acttgtttct tccagcactt ttggactgtc taacttttcc
    13261 gaaacatgtt ccattcttct caataaatag attttccgga tatttaatat tatcgtctta
    13321 atcactgcat agacaggtat gcaaatcagc atgcctaata ttcctgcgac acttcctgct
    13381 ccaatcaata ccaagatgat cgtcaaagga tggatattca ttgatttccc aaataacaac
    13441 ggcttgatca aatttccatc taattgttgg atcactaaaa tcgacaacgc catatacaaa
    13501 gcttgaaatg gagagataaa caaaccgaca atgattgctg gtaccgctcc aataaaaggg
    13561 ccgacatacg ggataatatt cgtgattccg caaatgaccg caagcaacaa gctataaggc
    13621 tgatcgaata ttctcattgc caaaaagctc attacaccaa taatcaaagc atctaagatc
    13681 gtactgctga tataggcaga taatgtttcg tttaattctt ttaccgtttg gcgtaactct
    13741 ttacgaatcg ctttcggaaa gaagtgagac aacgcatcaa taaatttgtg cccatccttg
    13801 aacataaata gcaaaataaa tggaacggta aataacaaaa taaaaaattg catcataaat
    13861 gaaatgattt ttgagacact tgcagataaa ctgactaata tgatattgaa caaattacta
    13921 agagataaat ttgctgatgc taattgttct tccaaattga aatttttaaa ctcttcccgc
    13981 tgcgccaatt catttaacca tttgcttgtt tcatctgcat acattggcaa gctctgggtc
    14041 aattgaattg tctgttccat taactgaggc actacattca tgacagttaa aacaactaaa
    14101 atgattacga ccaaaaacgt caataaaaaa ccgaacactc tcgggatccg atgcttttcg
    14161 agaaaaacaa cgattggatc gaacatataa tagataaaac cagcaacaag caatggtaaa
    14221 aaaactgccg aaagcatttt caatattgga ctaaaaatat tcggcatttg taaaataaag
    14281 tacacgccga tgatcgtgag tactagctcc actgtccaga aaaataattt tgattgttta
    14341 aaacgtgatt gcatacatct cctccaaggt cctttgatac ttctattcta tcatttaaca
    14401 aagagttacg cgctgagatt ttcatttgct taaaaaaaac aaaaaaaata aacatatttt
    14461 aatgagtaga cgagtactta aacaaaaaca cggtttggag tagtgacaaa aattatactg
    14521 cagtcccttt ttccaaataa acggtgttca aaagcttgca tcttcgtaat aagcccagcc
    14581 aaacgaaaaa tataaggaac tatttttatt tggctgggct tattacttga tgtaggacat
    14641 ctattttaca acctcagatc atttattgcg caatactgac taaatgcaac tttgtgatat
    14701 ttttgtgtac ggacaactcc tcgatcacat cggcataagt gagccctttt ggaagatcaa
    14761 tcgtatagat atttttgtac actcggtagt catcaacgaa ttttacgtca aaattcacat
    14821 cttcgattac gatatttttc tcttcaaaat aatcggtaag aaaatctttg gtttcttttc
    14881 tatggataaa ttggatttct aactttttag tcgtaggtac atggatcacg cgtttcacta
    14941 ctgtgagtgc aatagcaatc ccgataaaac tgatgatcgc aatggaatag taacccatgc
    15001 cgatggcgat ccctaatcca gcaattgccc ataaagaagc ggcagtcgta agtcccgtca
    15061 ccgattgttt cgtgacgatg atcgttcccg cgcctaagaa accgacacca ctcaccactt
    15121 gagcaatcag ccttgcttca tctgaacgaa tcgttccagc aagctgtgga tactctaaag
    15181 catctcttaa tgcaccacta gcgatctcaa cttggatcaa tgcaatgata catgctccca
    15241 tacaaactaa aatatgcgta cgcatacctg ctggacgatt tttatattgt cgttcaaaac
    15301 cgattgctcc gccaaatagc atagcgatcc ctaaacgtaa aataatctct ggaattgtca
    15361 ataccatatc attcatctaa acaccctcca gtccaatata tcatgtctga aaggagcaga
    15421 caaaaaaagt gcaattttta cctcttacga aaaaaggact gtgacaaaat ttttgtcata
    15481 gtccttttta tgaataaacg gtgttcaatc agctgaccct cggtattact caacaaacgg
    15541 caaaatatgg ggagctattt ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt
    15601 tcacaacctc tttagcctac tattgaaaat tacaacttac ttgctgcttc ttcatcgatg
    15661 atcacaacaa cattgtcgtg attttgcaat acacttgctg gcaaatgatt cgttacaggt
    15721 ccttcaatca tccctttgat tgcatcagct ttgctttcgc caaatgccat caagacgatt
    15781 tctttacttt tcataattga accgattccc attgaaacag cttttgttgg tacatcttct
    15841 gctttatcga agaatcgttt gtttgcattg attgttgatt ctgttaagtc aactactgaa
    15901 gtcgttccgt caaatgaagc accaggttca ttgaaaccga tatgtccatt ttgcccgatt
    15961 cctaagattt ggatatcgat tggatgtgca tcgatgattt gatcatactt tttgcatgct
    16021 gcttccagat cttctgcttt tccatctggt acaaacgttt ctttgaatgg ttttttatca
    16081 aataattgga catccatgaa atgacggtag ctttgttcat ctgaaccgcc tagtccaacg
    16141 tactcatcta agtttacaga agtcatttca ctgaaatcca aatcgcttga agtcatttct
    16201 ttatataaag taactggtgt gcttcctgtt gccaatccta atacttttgc acctttgttc
    16261 ataccttctt tgatgatttc aaatgctttt tttccgcctt cttcagcgtt ttttacacga
    16321 atgatttcca tagtgattgc tcctttatat tttggtatag cccaattata gcattctttt
    16381 tttcttttta caataattag tatagaccaa attgcatttt tttcattttc ttgacaataa
    16441 ataggaatcc gtgttaggtt tcataagatg gttcaatcaa acaatccatg ttgcatgaaa
    16501 accaagcaag tgattcactg tggacgcttt atgtctgcag tttttttatg aaaaaggagt
    16561 tattatgagt atactgacat taaacaacat tacccagcaa tttggcgaca aaatcctcta
    16621 tgaaggtgtc gatctgcaat taaatgctgg cgaacatctt gggctgattg gacaaaacgg
    16681 agccggtaaa agtacattga ttaaaatcat cactggtgag atcttaccag atgacggaca
    16741 gatcaattgg caaaaaaata ttcacaaagg ttacctcgat caatatgtgg aagtcgatga
    16801 aacattaaca atcgaagaat ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga
    16861 aaaaattgct tttctttacc aaacatatag cgagaccttc gatgattcct tgctggaaaa
    16921 agctgggaaa ctccaaacgg aattagacca aggagtgttt tatcaaatcg acaccttagt
    16981 tgctgagatg agcagcgggt tggggatcga tgtcttaggt ttagattcac tgttgaaaaa
    17041 tctaagtggt ggacaacgtt caaaggtgat tttagcaaaa ctacttttag aaaacccaga
    17101 tgttctgatc ttagatgaac caaccaatca cttagatgat cagcatatcc aatggctgac
    17161 gcagtttctt caggattttg acggtgcgta tatcgtgatt tcccatgacc aagaatttct
    17221 tgatcagatc accacccata tcgcagacat cgagtttgga aagattacta agtatactgg
    17281 ccatctaaaa caagcattga aacaaaagga acaaaatcgc gaaagctatt tgcgccaata
    17341 tcatgctcaa caaaaacaaa tcgaaaagac cgaagcttat attcgaaaat acaaagccgg
    17401 ttcccgatca acgatggcaa aaagccgaca aaaacaatta gataaaatcg aacggctgac
    17461 gcctccagct tcagcaagta aagctgtctt tgattttcca ttctcgccta ttgtcacgac
    17521 attagcagtg gaaacaactg atttagtcat cggatatgat cgaccgctac ttgcacccat
    17581 caatttgacc atccgctttg gtgaaaatat tgccattcga ggcttcaacg ggattggaaa
    17641 atctacttta ttaaaaacac tgatcggtga gattgaaaaa ataagtggcg atttccactt
    17701 cccagataac acaaaaatca attacttttc acaagatctg tactgggaaa atccattaga
    17761 aacaccacta caatatttaa gcagtcaatt tccaaaagcg actatcaaag aattacgtcg
    17821 ccaactagct aaagcagggt tagttaatca attagcaagt gaacctctca ctacattaag
    17881 tggtggggaa caaactaagg tgaaacttgc acagttgaca atgaaccaag gtaatttact
    17941 gattctcgat gaaccgacga accatatcga tcaagaaacg aaagaaagct tacaagacgg
    18001 gatacagaaa tttccaggaa cagtaatcat cgtctctcat gaacaagaat tttatcaaga
    18061 tcttgtgcaa cgagtcattg agattgaacc aaaataaacg tttgattgaa tagttgaaaa
    18121 aaaggagctg tgacaaaagt tttgtcacaa ctccttcttc tgaataaatg gtggccaatt
    18181 agctgacctt cggtattagc tcaaaaaacg gcaaaatatg aggagctatt tttatttggc
    18241 tattcttatt acttgatgca gaacagcttt tcacaacctc ttttcgtcta ttttttattt
    18301 tcgagatttc tctttccttt aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa
    18361 acaagcccgc taaccgttac acctaaccag ccatactgct gccacattgt cgtccctagc
    18421 aatgaaccag ccgcacctcc aataaagtac ataaacataa atacagtatt atttcggttg
    18481 cttgctgctt cacctagccc ttgtactcgt gtttgattgg cgacttgacc aaattgattc
    18541 ccgacgtcta ataaaataat agctaaaagc aaaatggcca aatggcttcc gccaaccaat
    18601 aaagcaataa agcttattgt ttgcataaca agtcccatat acacgatttt tctttcagaa
    18661 taagaatctg ccagtcgtcc cacgataggt gcagcaactg ctccagacaa accaaaaatc
    18721 gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg gactgctgat ataaaaaatc
    18781 aacgtagacc aaaagatcga aaatgttcca aacatgaaaa atccactgac tgccgcttct
    18841 cttaatagac gttgagaagc aactaaatgc ggcaaacttt taagtgaatc aaggtatgaa
    18901 acagctgtac tactttgctg gacagaacgc ggcattttcc agtaaagcag aagtgtaaga
    18961 attccgactg cacataaagc aaataaatag atcatccgcc aagaaaaaag actggcaaca
    19021 ataccagata ccgtccgaga aagcaaaatc cccacgagta aaccgctaag caatgtaccc
    19081 atgaccttcc ctcttgcttt tggtcccgct agtaccgcac cgtaaggaat gatgatttgc
    19141 ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg caaataacgt aaacgaaggc
    19201 gcaaaaaaag cggccaacag agagacggca gataacgctg ccattcgaat gatcaatttt
    19261 ggacgatcca ccacgtcccc taaaggaact aaaaacagta accctaacgc atagcctaat
    19321 tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa cagataaact agctgctact
    19381 tttgttccaa tcggctggat gtaatacata ttggcaacaa caacgccaca agtgattgat
    19441 aatagtaaaa taagcgaagt tgttaaaggt tttttttcat tactcttcat tgatttcccc
    19501 catttcctat cttagtttac aacttatgga aaagattgga aatagaaaaa tacaactatt
    19561 cgccacactt attatttgtg cttttgtttt ccttgttttt tcttgcgatc tttataaatg
    19621 aagacaacta accctactag cacagtaaca cttgcaacca acgtgactgc agttttgtta
    19681 tatccgtgaa acagagcttc tccaccacac gcatacaaca cagacgaagg gatgactcca
    19741 atcacgatcg acaatatgat ttgctgtaga ggcaatttca tcgcatctgc agcaaaattg
    19801 accatagaag atggaataac cggtatcata tagcctaaca ttacacctat ttttggatgt
    19861 ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg ttttcttatc taaaaatttt
    19921 aagcgatgca taaggaaaat tgcagtgatg ttaccaagag cattacccaa aatgttgatg
    19981 atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga ctccaactac tgaagtagga
    20041 atacctggaa cagcacacat caccgctgtc aatacgatca agagtatcga tgcaaaagtc
    20101 ccatgcgaac gaatatgtgc gagtagctct gccttactcg ctctcggatt taaaaataat
    20161 tggatgtctg tctgatattc taaatacaac cgatagccca ctaccagtat cagaatgatt
    20221 cccaacgaga tcaatatcaa tcggattatt ttctttttca tatttcctcc tgaaaaaaat
    20281 ctatattcat taaataatag tttaacataa aaacaaaaaa gcaccgaaat ccaacatagg
    20341 atctcagtgc ttttaaatag taaaaattat ttaacagtta cagatgcgcc aacttcttca
    20401 agtttagctt tcaattcttc agcttcttct ttagatacgc cttctttaac tggtgcagga
    20461 gcgccatcaa ctacagcttt agcttctttc aagcctaaac cagttgcttc acgaactgct
    20521 ttgataactt taactttttg gtctccagct gcagttaatt ctacagtgaa ttctgtttgt
    20581 tcttcagcag cagcagcgcc accagcagca gctacagcta caggagcagc agcagatacg
    20641 ccaaattctt cttcaatagc tttaactagg tcgttaagtt ctaggatagt tgcttctttt
    20701 aactcagcaa cgatgttttc aatgttcaat gccattataa tttcctccgt ttaattgttc
    20761 ttttttattt aatgctttat gcggcaatta agctgcatct ccatcttctt tgtctgccac
    20821 tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt agtacagata gcaacataga
    20881 tagaagtcct tcgcgatttg gcaattttgc caatgctgtg atttcttcca atgaagagac
    20941 tttaccttcg atgataccgc ctttgatttc taatgctttt gcttctttag caaattcgtc
    21001 catgattttc gctggtgcga ctacatcttc gttactgaat gcaacagctg taggtccagt
    21061 gaatacttcg tccatgcctt caaggccagc ttttttagct gcacgtgaaa gaatagaatt
    21121 tttgataact ttcatttcta cgttagcatc acgaagttgt ttacgtaaac gtgttacttc
    21181 atcaactgtt aatccgcggt agtcaacgac tactactgat gcagctgctg taaatttttc
    21241 gcttacttca tcaacgattg ctgctttttt tgcgataatt gcttcactca tttttgagtt
    21301 tcacctcctg aattttgatg gaagagtttt ttgcactgaa aaggcaagtc acttttatag
    21361 ttactttgct caaccagtat tattcatcac ctattcactt cgttcatagt gattctactc
    21421 gctttgctcg aaaggaaact cctttcgctt tgctcaacca gtattgttca tcatctcttc
    21481 acttcgttca tagtgattca caataaaaaa ctctatgcca ccgcagacat agagggacta
    21541 ttgaatatat tcaataaacg tcctcggtag gaaattaagg cttgatgcca cctactgtct
    21601 tcggtacaag ttaattatta acctcaacta gcttagcatg ctaaactagt tgagtcaaga
    21661 attatttttc agttggatca atcaacattc taatatttaa ttagaaagaa gcttggtcaa
    21721 catggatacc aggtccaaat gttgttgtga tcgttacatt tttcatgtat tgacctttag
    21781 ctgctgatgg tttagctttt agcaatacat catggattgc tgcaaagttt tcaactaatt
    21841 tttcatcgtc aaatgaaact tttccgattg gtacatggat atttccagct ttgtccacac
    21901 ggtaagttac tttaccagct ttcacttcgt ttactgcttt tgttacgtcc attgtaactg
    21961 taccagtttt agggtttggc attaagcctt ttggtcctaa tactcgtcct agacgaccta
    22021 cagtagccat catatcaggt gttgcaacaa ctacatcaaa gtcaaaccat ccgttttgga
    22081 ttttttgaac catgtcgtcg tcacctacga aatctgcacc agctgcttca gcttcttttg
    22141 ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt accagttccg tttggtaata
    22201 caacagcacc acggatttgt tggtctgctt ttttagggtc tacgttcaaa cggtatgcaa
    22261 cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt tgctaaagct actgcttcgg
    22321 caacaggata aactttactt gaatcaactt tttttaatgc atcttgcatt tttttgctct
    22381 ttttagccat tttggttcct ccttgattgt ggttataacg gttgtacctc ccacgtgttc
    22441 tatatctttc aatatagagc caactgagaa gctgctgtct tagggtgtat agattattct
    22501 acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc gcattgctgc ttcaacgtta
    22561 gctgcgttta ggtcttccat ttttaattca gcgatttctc ttacttgatc gctagaaact
    22621 ttcgcaactt tatttttgtt tggttcgcct gaaccttttt cgattttagc tgcttttttc
    22681 aatagaacag cagctggcgg tgtttttgta acgaatgtga atgaacggtc ttcgtataca
    22741 gaaataacaa ccggaatgat caaacctgct tgatcagctg tacgagcgtt gaattctttt
    22801 gtgaatccca tgatattgat acccgcttga cctagtgcag gacctactgg tggagctgga
    22861 gttgctttac ctgcaggaat ttgcaattta acgatttttt ctactttctt tgccacgaga
    22921 catacctcct taagtccgtg atgtggttaa ttggagatgc atatttctcc tcccacgttt
    22981 tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac aatgcacact ggtacagtat
    23041 agcaaattta tttgtttttt caagattttt ttctgctatt tttacatttt gcttgaataa
    23101 acgctaaatt tattctaaaa aaacaacaat ttttcgttct catttcttct tttatgttac
    23161 acttgcgcta gcatggttca acagatgaaa gcaaacaata acacggtttt ttagaccctt
    23221 gtacaaagat tcctacataa caaaatgagg tggagaaaat gaaagattta aggtatagaa
    23281 gtgtctttga tattattgga cctgtgatga ttggtccaag tagctctcac actgccggag
    23341 ctgctagaat cgggaaaatc atacgtagta tatttggcga acagcctgat tctgtagata
    23401 tttatctata tgaatcattt gcaaaaacct atcgggggca cggaacagat attgcattag
    23461 ttggcggcct gttagggatg gaacccgatg atgaacgttt agctgattct ttgaaattgg
    23521 catatgaagc cggtatggaa gtcttgtttg ttcctaagag tgaaaaagcc gaacatccaa
    23581 actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct ttcagtcact ggcatttcga
    23641 tcggcggtgg caatatccag atttctgaat tgaatggatt caaaatttct ttaagcatgg
    23701 gtactcctac cttgatcgtt gtccaccaag atgttccagg tatgattgct aaagtgacta
    23761 gtatactttc tgagaccaat acgaatatcg gcactatgac agtgacacgg gaatcaaaag
    23821 gtgaaaatgc cattatgata attgaagtag atgatcctca agtagaagaa acggtaaaaa
    23881 gactcaaaca gctgccaaat atcgacagcg taaattattt cgattaagga gctaaaaaaa
    23941 tgtttttatc gattgaagaa ttagtagatc aagcaaaagc ttatccaagt gtagcagaat
    24001 tgatgattgc agttgaaatg gaaatgagtg gccgttcccg tagtcacatc atcgagatca
    24061 tggaaaaaaa cctctcagta atggaacaat caatcaacga gggaaaagaa ggtgttacct
    24121 ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga tgattattta aatgaggcag
    24181 actttttaag tggagaaaca atcttaaaag ctgtacgcaa tgctgtagct gttaatgaag
    24241 tcaatgcaaa aatggggttg atttgtgcta caccgactgc cggaagcgcg ggtgttgttc
    24301 ctggtgtgct gatggctgtc cgagatcggc ttgatttgac acatgaacag caattggaat
    24361 tcttatttac cgctggcgct ttcgggttag tcatcgcaaa caacgcttct atcagtggtg
    24421 cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc aatggcagca gcagctttgg
    24481 tttgtgcaaa aaatgggact cctcatcaag cagcacaagc agtagccatt acattgaaaa
    24541 atatgatggg actgatttgc gatcccgttg caggcttagt tgaagtcccg tgtgtcaaac
    24601 gtaatgcttt aggctcttct caagctttta tctctgctga tatggcatta gcgggtatcg
    24661 aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta ccaagtcgga cgtcaaatgc
    24721 cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt tacgccaact gctaaacgac
    24781 ttgcggaaga gattttaaat aagagggcgt gataaagctc cttaatttcg agtattaaga
    24841 gattagttat cttcataaca aaaaacgaag agaggactaa ttgcccttct cttcgttttt
    24901 tattaatcca tcaatttcgt attttttact tcatcgatcg tttttgttcc tgcaagttgc
    24961 atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag atgttactcc ttctgcccca
    25021 cctagattca acccataaat cactggtcgg ccaatcgcaa ctaagtcagc accacttgcc
    25081 agagctttga agacatgctc tcctcgacgg acaccagagt cgaaaatcac tggtacacgt
    25141 ttgttgacta cttcggcaat ttttggcaat acttcaaagg acgcaggacc tccatctagt
    25201 tgacgtccac catgattgga tacccagata ccatctgccc ctgcagagat agcgattact
    25261 gcatcttctg gcgattgtat tccttttacg ataactggaa gatgggtgat ttctttgatt
    25321 gttttgatgt catctggtgt caagccttgt tttgcagctg cataaatttc tgcgattcct
    25381 tttccttcgc cattaccact tgcactttgt tcgctatacg cagctaagtt aggcattggt
    25441 aatggaaatt ggaattgatt gatgacgtct tcttcacggt aaccaccgag cgttgaatct
    25501 gccgttaaaa tgatagcttt agcccctgct tctactgctt tgttcaaaat gaactcattg
    25561 aatctgtcgt ctttactcat atataattgg aaaaactgcg gtgcgcctgg cgctgcatcg
    25621 gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg aaaagattga tccagcagca
    25681 gctactcctt tagctgtatc agcttcccct ttttcatgtg ccaatccttg ggccgctgat
    25741 ggcgcttgga taatcggtgt atctaattca attccgaaaa cagatgtatg caaatcggct
    25801 gagtcgattc cgcgtaatat ccgtggcatg attttctttg tattaaaaga agtcgtattt
    25861 tctttcatcg tccactcatc ttcagaacca ccgcgaatat agccaaaagc tcctgcttcc
    25921 atgcgctctt ttacacgacc ttctagttag gcaatgttta caatatcgat tggatgttct
    25981 tcattacttg tatgataagt catagtagat ccttctttca tcttcttgat tacatataaa
    26041 tcatactaaa cttacaaaaa gtaagcaaac atttactctc agatactttt atcgctattc
    26101 tttccaaata aatcgactta caagctgatc tacttcttgg ttttcatgaa gcatactatg
    26161 agaggcattt tcacctgtca ctactttttc ctctatttca tttccgcgtt gcttcaacaa
    26221 cgcatagacc gctaatgcac tgtttaacgg tactgttcca tcactcaagt ctgtttcatc
    26281 tagttgacca gcaatcaaga aaaatcgagt ggttatagga atattaccga tcatttgctg
    26341 gtaatcttga tagcggttag aaacgacagc aggccccttt gctaactcat ctgtcagaga
    26401 ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca attgctgtta atgtctgaac
    26461 ttgtggcaat gacgtatcct gtccatacgt tcctaaatag cgtagtgcac taactcctcc
    26521 catggaatgc cccactatgt tgactttatc tatgccgtag ttttctttca gatacaaaag
    26581 acaagttttg atccattctg cttgattcca ctcgttattt ttattatctt caaacaatac
    26641 ttggaccatc ggattgtctg cttttccaga caaatctcct gttgcttgta tttgtccatc
    26701 tgttcctact gttaaaacca attcttttac tgcttgctga tttttttcca aacgccgcaa
    26761 catcccttta aaggaatttt ctgtcccgct gtagccgtgg aaaaaaagtg ttggaatagt
    26821 ggttttttct atcactctac tggattctgc cgttttttct ttatcagaaa cttcagtagt
    26881 atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa ctgataagga ctaaagctgc
    26941 tgcagttgca cgtttgcata aatttttgat tggttctgtc atacacctct tcctttccat
    27001 atagaaaaaa gaaagacttg aaaggccaat catatgatcg acatttcaag tctctttatt
    27061 aatcgatttt atctacttga tcgaaatcaa gttctgtact ggtttcacgt ccaaacatat
    27121 cgatgttgac tttcaatttt tctttttctc cgtcaacttc tgttacttgt ccttctaatc
    27181 cagcaaatgc gccttcgatg attttcacga cttcgccaac ttctacttct agatcatttt
    27241 gtcgcaggct cataccgata gaacgcaaga tatgattgac ttcttctgga agtaatggtg
    27301 ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt cacaccaggt gtgttgcgga
    27361 caacatacca tgaagcgtct gtcatgatca tttcaacaag tacgtatcct gggaatgttt
    27421 tatgaacgat ttctttttct ttcccatttt tgacttcagt ttctttttct tcaggaacaa
    27481 cgacacggaa aataaagtcg gccattttca tactttgcgc acgagattcg atatttgctt
    27541 ttactttgtt ttcatatcct gaatatgtat gcaagacata ccagtttttt tcaaaagatt
    27601 ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc tcagtacacc cgaagttttt
    27661 ctttcgcttc attatagcac taaaagtacg ttgtggctag cgagttattt taaaatccag
    27721 ccaaataagc tttggatggc tgtatccatt acgtaaaaca tcaatccgaa aataatagaa
    27781 gtttcgatga ctactaacgt atctttacgg agttgttttc tgcttggcca agttacttgt
    27841 ttgatttcat cttttacgct ttttagaaat ttcatgagac aacctccctt gctactgttt
    27901 atttcgtttc tttatgtaat gtatattgat tacaatattt acaaaactta ttgatttcca
    27961 aacgttcgcc gctttttcct tcactgactg attttgtgta gttgcgagac ccacaaacgg
    28021 aacaagcgag tgctgctttt ttctttgcca tttacttctg cccccttgct gagaatcaga
    28081 cttatacttc ataacagtat cacgtaactt tttacttgtc aatttgtgaa ataaactatc
    28141 tattgttttg gataaaatta tgatatgatt aatttgaaat taagctaaaa tgagacaatt
    28201 ggaggtatag cgatgctatt aaaaacactt gtatataaga aaaaagatct gacaaccgtt
    28261 acagagacta gtacattaga agaagcatta aaaattttag aaacttccgg ataccgttgt
    28321 gtgccgatct tagattcttc aggtaatatt ttccgtggaa acatttacaa aatgcatatc
    28381 tatcgtcata aagcaaatgg cggtgatatg acgcttcctg tcactcatct tttgaaaaac
    28441 gctacaaagt tcatttacct ggatacttct ttcttcaaag tattctttac gatcaaagag
    28501 cttccttata ttgctgtcct tgacagtgaa aatcattttt atggtatttt gacacatagt
    28561 acgttgctta atatgctctc tcaatcttgg agcgtagaac aaggaagcta tgtactgaca
    28621 attgcttcca caggaaagca aggagatttg gctgctattg caaaaatcat ttcaaaacac
    28681 agcagcattg ccagctgtat tactcttgat atcgaaaaag acgaatacat tcgacgtaca
    28741 ttgatcacgc taccggctaa cactgaagcg aaagtatgtg aaacgatcgt caaccactta
    28801 gagaaaaaga attataaagt ggtagaaatt gaaaatttgc aaaaccaaga agcataatca
    28861 aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt cgctttttat aattagacac
    28921 gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa tggtgctgaa acagccatga
    28981 cgataaactc acttaatgca agcgtgccat aggttgtcca aaatgcttgg ccacctgttg
    29041 gaactaacat ataagcaatc agtcccatgc tgatggtaaa gaagatcgtg ttcaatacta
    29101 aacgcatctt tacatttttc actttgtttt gcaataaagc tgtcaggcct aaagaaagca
    29161 atgtttggcc gccaccaaaa accacatcca tgataccaaa accaaagaag gcattataga
    29221 cgatgacccc gcctaaaact ccccaaagca atttacggtt aaagacaact aaatgattca
    29281 aactttctga aatacgaaac tggatcggcc ctgaggatac aggcgcaact aaaatagtta
    29341 acgctagata aagagccatg atgatgccat ttacaacgat tacggataac cgttgtctgc
    29401 ttttcgtttt ttgcattttc tctcctcttt tctcctagtt tttttaacgc gggatggttg
    29461 atgaaccgcg gttatgtgaa acaaacaata attataacag acttttcatt tatttcaatc
    29521 cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg taaacgtttg ctaaaaatcg
    29581 tgggatatgg tataaagtaa gtgtaagggg tcataagaat tcccagtaaa gcagacgaag
    29641 taggatctgc aagaacagaa ttgttgtgaa acttaaataa ataaaaaagg atgtgggaat
    29701 ttgagcaacc tcttactaga gaacggtcta gcaagcagct gaagatctaa gtggacatgc
    29761 cgaaccttgg aacttctcct tacgtaagta atggatcatg cgcttgctag gcttttttgt
    29821 gtttttcggt cttcggacct ttttcatttt cctagacttc tgctataata accaagaatt
    29881 aattttgtaa cagatttttt tgaaaaaaca agtttctttc attcaaaaaa tagaaaaagc
    29941 ttgctaatta ttggttaacc agcaaacagg agtattaaaa tatcttgttt tattgaatag
    30001 caggaggaaa tcatgaaatt ccattatcat atattgatca atccatcggc aggcagcgga
    30061 aatggctata aagtagcaga aagaatttta ccggtattaa aaaataagca tattgactat
    30121 acacttcatt atagtgaata caaaggacac gatgctgaga ttgctgaaac tttggcaaaa
    30181 gaaacattga ttccttggat agaagaagaa cagaaaacaa tcgatacttt cccccttttg
    30241 atcatcgtag gaggtgacgg caccttacat caggtacttg atactttcta tcagatggaa
    30301 gtagaattcc cagttgctta tattccagca ggttctggag atgattttgc tcgaggggca
    30361 ggccttccaa aaaatcccaa aaaaggactt cagctgattt tagcagctca atcaccagaa
    30421 aaagtccaca taatggctta tgaagaaaaa ataagtgaaa aaaaaggaat agctgttaat
    30481 aatttcggta taggattaga tgcagctatc gtccatgcga caaatcattc ttccacaaaa
    30541 aagcgattga acaaatacaa cttaggatca ttttcgtatt tattctctat tctccgtgct
    30601 ttattcactc aaaaaggatt tccaattctg gtagatgcag gaggaaaaca attaagtttt
    30661 tcaaacgcat ttttatgtac agcaacgaag catccttttt ttggcggagg aatcgcgatt
    30721 gttccaactg ctgatgcgtc taaaccagtc attgattttg tcgtggtcga acgaatcaat
    30781 ttctttaaaa tcttgtggct cattctttta cttatacaga aaaaacaatt gaaatcgaaa
    30841 tatttccatc actacacaac aaacagatta cgtatcgttt caacaacacc gcaacatggt
    30901 caagaagatg gagaagaaat ggaaaaacga ccttttgata tcacaataac cacaactaaa
    30961 cagttgtttt ggtgttaata aaagaggttt tgtcagaagc tacatctgga acaccgttta
    31021 ttcggaaaaa aggactgtga cacgactttt gtcacagtcc tttttttact atgcagttaa
    31081 ttctttgccc aattgggttg ccatcgatag tttctttctt tacccaaaat aaattggatc
    31141 attgaaaaca ccgcagcaaa agcaactaag acacaaccat tgaaccaaaa agattctgca
    31201 aatgtttgat tgtaactgag tgtttcactg atcaagttat tattttgaac aaaccaatat
    31261 gttggtgtga ataccgctat tttgctaacc atttctggca agtattctgc tggtacaaac
    31321 accccaccga taaagcaact gcctgtgata aaaatatctt tgatcccacc gctggtttct
    31381 gagtttttta ccaatgaagt gatacaagcc gaaaagctaa tgatcggcaa taaaaacaag
    31441 aaagtattta aaatatgata ccataccgtt atatcccacg aggtgtggat cactaacaga
    31501 accattagaa gtaagaagcc actgaacaac agcagcgagt aactaatcaa actaaaggtt
    31561 actttgcgat tcaagtttcg tttggtaatg ggcgcactct ggtttcgttt actgatctct
    31621 ttgcgattaa atgctagatt gatgacgctg aaaccgctaa aaatggatag aaacaaccca
    31681 taagacaaga aattaaaggt tgtgcccgtc aacagctttt tcatccgctg actatatccc
    31741 gcacttagct caacttctcc agtaaccgag agtgccattt gtgtctgttc catagctgca
    31801 tcgattgttt tatcttcttc ttgaaacaga atcagtgttt gcaaaaaggt gttcacttgc
    31861 tgtgtaacaa ctgttttaga aaaggcatct gggcgactct gtatcttgat cgtcggcatc
    31921 tttcctactt cgacttgttc ggtaaagtct gcaggcagat gtaagataat gtttacacga
    31981 ttaaaataaa gagcatcatc gatttctttt tgtgaggttc catccaatgt aacgactgtt
    32041 tccttgtcat tcaaatattt acgaaggcca tcgactacag gcgactcttc ttcactaatc
    32101 aaggcgattt tcgcatcttg cagagaggta ttttcaacat caatattttg cgcaaaaaag
    32161 aatgtggcaa caacagtgat aattacacct atcagtaaat tcgatttatt tgctttaagc
    32221 aatgtccaaa tcgttttata aagcgtcata acgcacccct ctttccatcc aagcatttcc
    32281 taacgcaaaa aggaaaataa atccgatcaa ccatagaaga ttggtataaa aactagccaa
    32341 atcagtgtag aaataaagtt tatacaaact ttcgctgatc aagttgacga gatttaattg
    32401 accaacaatc ggaatatgaa gatcaatcca atacttcact tgttccgacc ccatcatacc
    32461 agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg ctatcctttt gcaccagatt
    32521 catttttggt atcaaattac caaccaatgt acctaacaaa atcgcacaca acgaccccag
    32581 agcggtaaca atcatcaacc attgccaacg ttcaccaaag tctacttgat agataaaacg
    32641 aaagacagct aacataatat aaatctctgt caaaacaatc gtaaagcttg ctgccaaatt
    32701 agcgattgaa actaacgatt gctttctagg aatcaaggac aagcgaatac catttgacga
    32761 ttgatttgct tgctgatcct gtacattgcg gaccccccac atcgtcccgt agagaatcgc
    32821 cattccaatc agagtgaaga aataaaaact tttgaaactc atattacgac ctgcatgaac
    32881 ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg ggcgcgagat caacgttctc
    32941 cacttctgcc actttactcg aaattatacc aatacgctgt aaaaattgat ccatgacgtt
    33001 tttcatcaac gtctgactca ttcctgcttg acccacatgg agcgaaacag atgaaccatc
    33061 aatgagataa aaaccagcca aatcatctgc atttagcttt tcattcgctt ctacttccga
    33121 tagttcctgt acttcaaata atggtttatc attgacttcg acttgttcta agactgtggt
    33181 aaaattttcc gaaagcgctg tttgatccgc tttgattaca attccagtat taatcgtttc
    33241 gagattgcca ccttgatcaa tctcgccaaa ggccgccata aacatcaatc ctaaaagaat
    33301 tggaaaggcc aatgtccaaa acaacatgga tttatttcgc agcagtattt ttgtgcgata
    33361 tttataaaaa tgccaaaaca ttatttcccc tcctctagtc tctcaaatcc ttgccagtaa
    33421 tctctaaaaa gacatcattc aaggacgctt ctttggttgc tatttttccg taactgatct
    33481 tttcttcttg taagattgta agaatcggca acaatactgt atttagttca gtagttgtta
    33541 cttggagctg acctttttta taagcgattc catcagaagc tattgttcgc tgacaacacg
    33601 ttttctggga gtaatgggat gtcgatcgtc aaacgatccg ccgtccgtac catatctttt
    33661 agagattctt tggttccttt agcaatcacc tgtccttgat ccatgatcac gatctggtcg
    33721 caaagaattt ctacttcttc catgtaatgt gttgtataga caatggttgc tccttgtcga
    33781 ttcagctcct tgatactttc taaaatttta ttccgacttt gggggtctac agctactgtc
    33841 ggctcatcta aaaaaattaa ccgcggcttg tgggcaatcc cacatgcgat gttcaaccga
    33901 cgtttcaagc caccactaag ttctcctgga cgaaacgaaa catatttttc taatcccact
    33961 aattgaatca cctcttcaat caaggctttc cttttctctt tttcggaaac atataaaccg
    34021 caaaaataat cgatattttc ttgcacactt agttcgtcaa agaccgcaac gtcttgagga
    34081 acgaccccaa tcattgcctt gacagcataa ttttcaggag tcattgttgt cccgaacaat
    34141 ttaatggttc ctttgtcata atttaacaaa gaaagcatac agttgatggc agtcgatttc
    34201 ccactgccgt ttggtcctaa cagacctaag acttctcctt cttgtacttg cagatcaaaa
    34261 tgattcaatg ctacaaaatc tccatagcgt ttgactaatt tatcaatttc gataatcatc
    34321 atgaaaaact ccttccaaat ttcttgttga tctgactata ataaagtgca agaaaagaaa
    34381 ctagtgacga ttggctaaac cacacatgac atttgtcaca atgaaggagt ctgctatgca
    34441 taaatctcgg ctactcaacg aaactagctt tctactggtt atctcattgc ttttgctggt
    34501 aagtcaagga ttcgttgcta tgcaagttat ctatttattg atcgccgttt tattcactgg
    34561 tgtcagttat ttactgccta caaagccaaa ttggattttc caagttattc tatttctttt
    34621 atgtgccttt ttacctgatc ttgtcctgtt tctgccagcg acgatgcgag tgatcgcccc
    34681 tgaagaaagc caccttgatt acttgttttt gttaggtttt ttgttgattt cgacggttgg
    34741 taatcatccc cccatcaagg tcgcctttgt cgtaattttg ctgttttttt ccttttactt
    34801 acgaaaaaaa gatcaacgaa cgaccgatct tgagaaaaag tacattgtct ccaaagatac
    34861 tcattgggaa aaacaacaag agttatcgga acaaaataaa gcattggccc tgtcacaaga
    34921 aacacaatta gccttgcaaa ttgtggaaga acggaaccga attgcccgag acatccatga
    34981 caatgtcggt catctgctgt caagtgcgat tttacaaatt ggtgcgcttg aaatgatcaa
    35041 tcaagaacca aaactaaaag aaccattaga aaatttaagt gccaccgttc acactgggat
    35101 ggatcggatt cgtcaaagtg tccatgacct ccatgagaca tcgatctctt ttcaacactc
    35161 cttagagttt ctgatcgctg attttccttc ttctgttacg attgaaggaa acttgtttga
    35221 aggattgaat gaaacgcagc aaaactgctt cttaatggtc atcaaagaag ccttaaccaa
    35281 cagtatcaaa catagtcatg ccaaaaccgt tgttttacat tttcgaacgt tgccggcctt
    35341 ttatcgcttg caaatcacga atgatggcac cagcccacaa aaaaattctg ctcccggtat
    35401 tggtttgaaa acgatgcgtc aacgtgttca agaacaaaag ggacaagtcc atattttcca
    35461 agaaaaccag caatttcagt taacgatcat tttaccaagg gagggacacc aatgataaca
    35521 gtagtcatta ttgagatgat ccatttgtga cgaaatcgtt acaaaccatt ttagaaagca
    35581 cgaaagagat ccgtgtgtta ggcattggtc attgtgccaa ggatgcgcta gtcttatacg
    35641 aaacccatcg tcccgatgtt ttgctgacgg acatccgcat gcctaaacaa acgggcatcg
    35701 atgctgccaa agaaattttg gctaaatttc ccaaagccat ccttttgctt ttgacgacat
    35761 tcaaagatga agaatacatc cgcgaagcat tttccatcgg tgtcaaaggg tatttaatca
    35821 aacagaactt gcaagcgatt attccttctg tcaaatccgc ttataatgga caggccgtat
    35881 ttggtaatga aatcatcgag actttcactc aaatgatgaa gaataaaccg acgattgata
    35941 gtcatgctta tgcttctttt agtgaacgtg agcttgcaat catcaaagaa gtagctgccg
    36001 ggaaaaacaa taaagaaatt gcagatgcct tgtacttgag tgacggcact gttcgcaatt
    36061 acatcagcca attactagaa aagctagatt tacgggatcg cacgcaattg gctatctatt
    36121 tttatcagca tttgcaataa gtcttatcta acacaaaaaa acctgaatga tgctccttcg
    36181 catcattcag gtttttttca catcttctat tcaattgtcc cgcaaaccat ttcctcacga
    36241 ttgaaagaat caaggattcg cagggctgcc aggattcgtt ggattgttcg gcgtgttagg
    36301 cggaaaaata ggcgccttgt ctaacgtttt ttctagtttt gattcttcac gatcgtctcg
    36361 ttctacatca ggactttcat tcaatttgaa ttctttttcc atctcatcta actttttaaa
    36421 gtcattgcca gattcttttg ctaatttcgc taatttatct tcggtttctt tagccataaa
    36481 gtaacgcccc ccttttgatt tcagcttagc atatcacact gaaaaaacaa aagaaaacgc
    36541 taaccaacga tcatttttag ctatttctca tctttaaaat caagcatttt ttgccggtat
    36601 aaaatcgtag ggagaactgt tttccatacc aatcatcgga tctactaact ggttatcgat
    36661 cacttctgct gtaagaacga aatctttaga aacagcaggt tctacctctt ctttcttttc
    36721 ttctccaaca aatgaagaaa acagatctgg ttccactgtt tccattactt ggtttttcgt
    36781 ttcattcgtc ccactgtcat cttctacagg tagcttttct tttacaaccc gtggctcttt
    36841 tgtatagttc ccatcttcaa atggatcatt tccgaccaaa gaatcttcat aagctgctag
    36901 tttggtccgg agagtgatat tcattttatc attatccatg atccttgatt tcaacgtagt
    36961 catccggctt gctgattcat tcgctacaca agtgacatag gcttgctctt cccctaacaa
    37021 aatcaacagt tccttactgc gagtcactgc cgtgtagagc aaattacgct gaagcatccg
    37081 ctgatattga tggaccatgg gcaaaataac catcttgaat tcactgcctt gtgatttatg
    37141 gattgagcaa caataagaaa gagtgatttt attccattcg tttcttttat aagtgacctc
    37201 atttgcatca aactggataa ctaattcatc cactttatct tcagaatctt ttgccaagat
    37261 gatacccgtg atttctccca tatctccatt gaatacattc aattcgggag tattgactaa
    37321 ttggaggact ttgtctccta tccgataaac ggtgtcgttc caatgtactt ccttttttcc
    37381 aggtttccct ggattgaata tctcctgcat cattttattt aacgcatcga tccctgcagg
    37441 tccccgatac attggtgcta atacttggat atcttgtgca cggaatccct tatttttcgc
    37501 tttttccaca attttagaga tcaagggttc aatttgataa gcatcacttc ggaaatagga
    37561 acgatctttt tgattctttg tgaaatcttt tggcagacgt ccttctttga tctcatgggc
    37621 aagaggaata atactagaac catcaccttg ccgataaatc tgattgagtt ccattttggg
    37681 aatttgttcg atttccaaaa gatcatggag tacttgacca ggaccgacag agggaagctg
    37741 atctttgtct cctactaaga taacttgcat attcgtcgga atcgctttga ataatgtgtt
    37801 agccagccat gtatcgacca tagacatctc atcaacaatc aaaagtccgc cttctaattc
    37861 tttcgtactg actgaaggtg ttttttcccg tccattcagc cccagaagac gatggatcgt
    37921 actggcaggc agacctgtcg tttcgttcat tcgtttagct gctcggccag ttggtgctgc
    37981 aagtagaata ggaaaaattt cttgggtata ttttgaagga tccaattcca gtccgttcaa
    38041 ctctgcaaaa agattgacga ttccgttgat cacagtagtt ttccccgtac ctggtcctcc
    38101 tgttaaaata aatagagggg aacggattgc ttcagcgata gcttgttctt gcgaaggtcc
    38161 atactgtatg ccgaatattt tttcgatacg ccgaatattc ttctgcactt ctttttcagg
    38221 ataatggatt tcttttttac gactgagaag gcgctggatc gaattaccga tcccccattc
    38281 agaaaagtac aaactgtttt cgtaaagatt agtctcttct tgttgtatct ttccttcttc
    38341 aacaagttgg ataattacag tagcaacctg atcaggctgg atttcaattg ggcgacttgt
    38401 ttctaaaata cggatcgttt gctgaagcaa tgtctccgcc ggtacgtatg tatctcctgt
    38461 ttgaactgca tgctgaaaaa tctgatgcaa gatagctgct cgaattcttc tcggtgaatc
    38521 tgctgcaata ccgatttgtt cggctagatt atctgcacgt ttgaacccga ttccttctat
    38581 gtcttcgact agctggtacg gattttcttc gatgatatcc aaagactcgt tcttataggc
    38641 ctggtagata ctgaaagcta actgactacc gaacccgtaa cgattcagtc ccatgatcac
    38701 ttgatccatc ccgtggttta gccgaatcgt ctcagcaagc atttctcttt ttggcttcgt
    38761 caaaccggag atttgtttta ataccgaagg atcagcaatg attttttcga tagcatcttc
    38821 tcccagcaat tctacaattt tttctgctgt tttcttcccg attcctggga atttttcgct
    38881 ggaaagatac gctacgatcc catttgcagt tgttggcttt tcttgctcat atctttcgag
    38941 tttaagctgc cttccgtatt taggatgatc cactaattct ccataaaaac gataagtctc
    39001 attttcttga atatcgccga aacttccggt gactacaatc tctttttcac ggtaatcagc
    39061 tgtggtttca ctgatgtgta ccaaaagcac tttgtaaaaa ttactaggat tttgaaaaaa
    39121 aatcgcggca actgtaccaa cgaaataagg catttcttct ggctgcatcg gaagccgcct
    39181 ccttcttcta tagtaaaggt tctggtgctg tttcagttcc taaaaaatct gtttggttcc
    39241 atactttcag ttcatatggc aataatctgc aaggctcttc agtctctaaa atcgtcaaac
    39301 tactattgaa caatccaccc atttcacgca actcacttag ctcttttcct gtcatccatt
    39361 gaatggctgc agttaaagaa gctccatgtc ctacgaataa aagtgggcca tctttatgtt
    39421 gggcaaccgc atctgctaca acagtttcga tacgtgtgat tgccgattca atcgtttctc
    39481 cgtgaaaagg agaaggatca tacagatcta aatgattacg caaattcggc agattatccg
    39541 gatatttctg gtacatttca tcgatcgact gaccttctaa agtacccagc cctagttctc
    39601 ttagcttatc tgtataaaaa atctctaccg gacgagcaag ctctttgttg ataccttctg
    39661 ctgtatcacg tgctcgcttt gctgtactgg aataaattgc tttaaaagga acatatttta
    39721 cctttttccc aaatgctttg atttcttcgt aactttgtgg caaaagcgga gaatcgccat
    39781 tgctcccttg aaaacgtctt tcctgattcc attgtgtctt cccatgtcgg gtaaaatata
    39841 gttccatacg cagcaaactc ctgtctttct tttattttac tctataaaaa actgtctaat
    39901 ccagcttgtc attttcatag tgatgcgtca attctaaatt agggaatcct tgctggcgaa
    39961 gtgcttcata cactatcatt gcagctgtat tcgataaatt caatgagcgg acatgctcat
    40021 cattcatagg gatgcgtaaa cacttttctt cgttttcacg cataaatgtt tctggcaaac
    40081 cggttgtttc ttttccaaat aaaaagaagt gctcttctcc atcagcaaaa ttttcgtcac
    40141 tatatacttt atgggcaaat tttgagatca aatgtagttt acgattacct aaaaatgtta
    40201 aaaaagcttc taaattactg tggtaagtaa tatttacatc attccaataa tccaaccctg
    40261 cccgttttag atgtttgtca tcagttgaaa aacctagtgg ctcgattaaa tgaagcggag
    40321 tattcgtggc tgcacacgta cgggcaatat ttccagtgtt tgcagggatt aatggttcaa
    40381 ataaaacgat atgattcatc aggatcattc ctctcatatt tatgttcgtt tctattttct
    40441 gacaatttga tttttgcata aaaaaaaacg tatcaactcg gtgtcagtca ctgcgagtcg
    40501 ttacgttagt gaagctcttc tcactaactt ttatcagaac aggaacctga taaaaaccaa
    40561 tgaaaaaaca acttgttgct aatataacac cctcaacttt tttttgcaac ctctactcat
    40621 taaaaactgc tagaaaaacg ttttctctta gttcctttta ataattctat gtcaattgta
    40681 atcttcttca aaggattctt atataagtct gctttgattc gctcttctac ctgccattct
    40741 aaaggtgaca tggccaaaag atctttttga ttttcctctg gtatctcaaa ttcataggtt
    40801 acacgctgac gttcagttaa tttcattgct tcagcaaact tggctactac acgttcattg
    40861 gaataactct gtttttcagg ttgattcgga taaaaagcct gacgtagttc ctttaaataa
    40921 ttttcttgtg gcaccacctt caacacttct ccatcttctg agagtacacg ttggaattct
    40981 tggtaatgag aaggcgaaaa gatatttaaa accgcgttga atgtttgatc agcaaacggc
    41041 aaattggtca aatcagctac acaccaaaat gcttcggcag attgttctgt agctgcgtag
    41101 actccttctt ttgaaatatc aaatccaaca gcattttgaa tctttttcaa ttttgaaagt
    41161 tcattcaaga agctaccttc tccgcagcca acatctaata gcgagacttc ttcaggtaaa
    41221 tgctgcgcaa tagtctcaat aacgggctga tacatgccag attggatcat tctacgcctt
    41281 ggaacgaaca ttccttgatc gtattctgtt ttaaccgcat gggataaaaa ataaagtgtt
    41341 ccttttttgg aaaggtcaaa acggtgatta tttttgcata tcaagccatt atcagacttt
    41401 tttacttctt cttggcaaac cggacagcgg aacagctgat gattttctgc caaaaactgg
    41461 cgtcctcggt caattttttt taacatagtt ccccctcatt tctgttttat cttaccgtta
    41521 gatgctcact ttggcaactg ctaaaaatat gatacaatgc ctagaagaaa gtgaaagata
    41581 aaggagaaga ttagatgatc aaagaatttt gcgctgaaaa ctacacatct attcctctag
    41641 caattgctag aggagccgat cggatcgaat tgtgtgacaa cttagcggtc ggtggcacaa
    41701 ccccgagtac tggtgtgatc gaagaaacgt taagttatgc tggggaaaaa gatatccccg
    41761 taatgacgat tattcgtcct agagggggcg acttcgtcta taacgatatc gaattgaaaa
    41821 tcatgcatac tgatttgatt gaagcaaaga aattagggac ggatggcgta gtatttggtt
    41881 gtcttactcc ttcaggctgg ctagacgaag aggccttaga actattgatc gaaacagcag
    41941 aagggttgca gatcaccttc catatggctt ttgacagtat tcctgaagat cggcaattcg
    42001 aagcaatcga ttggctggta gaacatggtg ttgaccgtat cttgactcat ggcggtcctg
    42061 ctgacaaaac tattgtagaa aatttcccac ggcttaaaga attgattgcc tatacagatg
    42121 gccgcttaat cattttgcct ggtggtggtg tcactgcaga gaatgcagca catgtagcta
    42181 aagaattgaa cgtaagcgaa gtacacggaa ccaaaatcgt ggatcttcag ttataatctt
    42241 ataaacaaaa aaacatttgc aacaggcata atgcttattg caaatgtttt tgttttttta
    42301 cataaaatag cccataaata aagaagcaat attgaaataa atcagtacac tcgtcaaatc
    42361 gctcaaagtc gtgataaagg gaccgcttgc tactgctgga tcaaatccta gacgatccat
    42421 tagcatcggg attaaactac ctgcaagatt cgccactgtg attgcaaata gcatagccat
    42481 ccctatgaca aaccccaaga taaaattatg tttccaaaaa cctacgatta gaaaaatcgt
    42541 gatccccgta acagctcctg tgacaacgcc ggtcaagact tcagccaaaa caagtcgaac
    42601 aaatccatga tctttctcat cagaaatcgc aagtcgacga acagccacag ctagcgactg
    42661 ggttccagca ttaccggcag ttcctgtaat cagcgaaatg aagactgcta aaatactcgc
    42721 ttcacttacc aagtcttcat agtgactgat taaagaagcg gtcgacatac ctaaaaacaa
    42781 cagagtgatc agccaaggca aacgtcgaga cgctgccttt actggatttt cattaatttc
    42841 ttccacattg accccggcaa gtcctgaata atcgctagca gcttcatcat caataacgtc
    42901 gatgatatca tcgactgtga cgattcctaa caaatgatca tcataatcgg taacaggaag
    42961 agctaagaag tcataatctc taaatgtttg tgccacatct tcttggtcgt cgcctacatg
    43021 aactgaaagc actcgttcgc tcatcagatc agaaatcatt gcgtcgtcat cattcacgat
    43081 taggtcgcgc agagaaataa cccctactag ctgaccttct tgattaacga catagatgta
    43141 atagattgtt tcagctacat ctgcttcgtt cttcagcacg tacatcgcag agcgaacagt
    43201 ttgattggct acgatcgaaa caaattcagt tgtcatgatc gcgccagccg tttcatcctc
    43261 gtaatggaga agttccttga tttcactggc atcgtctgcc gacatcaaac ttaaatattt
    43321 agcaatttgc tttttatcca gcgtattaag caagtcaaca gcattatctg tatacatctc
    43381 agaaagcata tcagctgcat agctaggacg catttcatct aggtattctt tcatatgttc
    43441 gttgtcttct tcaatcacat cgaacatatc cgctaattct ttaggcgaca gataggaata
    43501 gatcagctgc ctgttctctt ctgtaagaga ttggtagaat tgcccctgct catagatatg
    43561 catctctaaa aaactgtttc tgaatgcctg aatattattt tcccttaaat cttgagtcaa
    43621 ctgggcaaat ctttcctcta attcttgatt ttcatccaaa ccaatctcac ctcgccttat
    43681 tcttcaaacc gtctcacaac tgcctccatg tctgcggcta aaggctgctt gaactccagc
    43741 aactcttctg taaatggatg gtaaaaacgc agatcataac aatgaagggc ttgtcttttt
    43801 attcccagat ccattcgccc gccatacata tcatcaccga gcagggcaca gccaattgcg
    43861 gaaaaatgaa ctcttatttg atgtgtcctt cctgtatgca gctggatatc aactaacgcg
    43921 atttctgtta gtcgcttctc tatccagtac tcagtctgtg ccgaacgtcc ttcatcagag
    43981 accattcgct tcaatagcga agaataatct cgggcgatcg gcaaatcgat atgtccatgt
    44041 gccgataaat ctgaaagcct tccactgact aaggcctgat attttttcac aaacttcttt
    44101 tcacgaagtt gcatatctaa ttttgcgtgc gcaaatccat gctttgcaaa aagcatcaaa
    44161 ccagatgtat cccgatctag tcgcgtcacc acatggatta cttggttttc gtaaccttgt
    44221 tttttataat aagcctttac acgattagcc attgtcccat ttggatggta ttgggcagga
    44281 atagacgaaa cgcctgctgg tttatttact accagaacgt gttcatcttc atacacgata
    44341 tcgatcggtg tttcatccaa aagtacggtt tcatgttctc cttcagcagg aatcacaatg
    44401 gttaccttat catccttagc tagagaaaac aagacatttt ctacttgatc attaactttg
    44461 atttgaccgc cttgaaattt tattttcgct aacagaccct ttgagatccc tttttctttt
    44521 aaaaaatatt ttacttgctg agaatgatct ttctcataat cccatgtaaa ctgcatatca
    44581 atcctcacta ataaaggcat ctttcacacg atgccaaaaa tgcatatgtc gataagaagc
    44641 aaaatggatc cgttcttctg ctattcgata ataaattgag cggatatttg cttttgcgac
    44701 attcaattga tcaaccgtta caagataatc ttctgtattt tccaatttta tttctagcca
    44761 ttcatcatgg gcaatcacga tcggagaacc caaagtacga aacactcgat tattcagtga
    44821 agcaatctct gccaactgaa aggcattgat actaggatgg atcacagcac cgccgacact
    44881 tttattatat gctgtcgaac cagtaggcgt agaaatcgtc aatccatctc ctctaaaact
    44941 ttcaaacagc tcgtttttga tgtatacatc ggctaccatc gtacgattgg cccgtttaat
    45001 cgttgattca ttcagtgcta aaaaatgctg gtccggtgtt tcatccaaat aactgatccg
    45061 aacatccagc aagggataac taacactttg ttcccggttc gttcgtaagc tgtctactag
    45121 ttcttccagc tcgtaatccc gccaatccgt gtaaaaccct aaatgacctg tatgcacgcc
    45181 caagaaacgc acatcattca gacaatgact gtagtgatgg aacgccgaca ataatgtgcc
    45241 gtctccgccg actgagatga ccaattctgg ctcatgatca tctcgttgaa tccctgcttg
    45301 ttctaaaagt gcgttcaaac gttctgtcac ttctatagtc tttgcttctt tattatgaac
    45361 gatcgctact ttcatcccct gcctcctctc tttcgctttc tttcttcaaa agcgttcgat
    45421 agtagacatc atctgatttt cctcttccat gggagaataa ccgctgtgct tcttggattt
    45481 cttcacggat actggacatc tcttcatcca actgataggc agcttctgcc gcacgctgaa
    45541 gtcgttcact catttcttct ggaaaaacgc cttgatattt ataattcagc gaatgctcaa
    45601 tcgttgccca gaaattcatc gcaagagtcc gaatctggat ttccgccaaa attttcttct
    45661 caccgctgat cagctgtaca ggatactcga tcaccagatg ataagaacgg tagccacttg
    45721 cttttttatt tgtgatgtaa tctcgttctt ggagaacttt catgtctttt cttttacgga
    45781 tgatctcaac aacttgatgg atatcttcta caaactgaca catgatcctt aacccggcaa
    45841 tatcagacat ttcttcttct aaacgtccca ttggaatatg ccgtaattgt gattttgtta
    45901 caatactatc gactggcttg actctcccag tgacaaattc aatcgggaca tgtcgatttt
    45961 gttcacggaa ctgtttgcgg atcccccgca gtttcacttt caattctgat acagtttgtt
    46021 catatggtgc taaaaatgct tcccaatctc gttccatagc tgatacctct acttcattaa
    46081 cattcattgt ttattctacc atatatgcaa agttttttcg tactcttgat ttatgcattg
    46141 accaaaaaaa gaaaattagg caaaatagac aaaaaggagt cgatcaaatg acacaaatgc
    46201 ttgaaatcga atataaatct atgctgacaa agcaagagta cgagatattc atcgctcact
    46261 atcaattaac agataaagat ttccgtgtac agactaatat ttatttcgat accgttgatg
    46321 aacagctgaa gaaacaaaat tgtggcctac ggatccgttt cgttggtcat caagctgaat
    46381 atacattaaa aacacctgct gaaaaagggc gtctcgaaac aacggatacc tttataacag
    46441 aagaagcaga agcatttatc ttcgaaaaac gtctgcctcg ttcaggcgca gtttttcaaa
    46501 aactttctga tttgaatatc gatcctgctt cattaatcca aaccggaaag cttacaacaa
    46561 aacgggctga attccctatt gaagaaggac ttttagctat tgatgaaagc tggggagaca
    46621 atctacacga ttttgaactg gaactagagg taggggatgc atctgtggga aaaattgctt
    46681 ttataaattt tttaaaacgt ttttctttac cttatcgtcc tgctaagaac aagatccaac
    46741 gtatgcttga agctaaaaat tagaaacctt tcattttcac aaggcacttg agttcatttt
    46801 tttacttttt tctgttaagt tataggatgt gaaagaatct agtcactagt tgtatagaat
    46861 ctattttggg ggagattcat atgatcgaaa tctatttatt tgttaatcct ttaggggaat
    46921 actgtttcga aatggaacaa cagttattac aatttatcga agaagagtac gggacaccat
    46981 caaaagaaaa gatgcaattt cgctttcttc ctttggtcaa tttgcagaca attggggatg
    47041 tcatgcaaca aaaaggaatt tctcagaaca atcttgagga acgtaatcac ttattctcaa
    47101 tgacttattc tgcagcattg gactgcaaag cggcgcagtt tcaaggaaag aaaaaaggcc
    47161 gccgtttttt gatcaaattg caagaagcag tcggctgtaa tgacatacca tattctcaag
    47221 aacttgctga atccatattt gaagaaatcg gcggcgattt gaatatgttt aaagaagatc
    47281 gtcagtctga cttcgttaaa gaagtattct tttctgatca aaaaattgct cgggaaatgg
    47341 gcatcacaaa acacccttct gcagtcgttt acaattatgc ctgcgaccgt gattttggtg
    47401 ttttagtaga agtaaaaggt acaagtagcc tttggagaat cacagagctg tgtaaaacaa
    47461 cagataactc atatcaagtt tttaatggtg aacatttaca tacaaatgat tctcagttcc
    47521 attcaagaac tagccattta cacttgcttt ctaattaaaa aaatacgtcg actcccttac
    47581 aagagagccg acgtattttt ttcgtttcta ttcgagaaaa tcaaaaatag aaagccttat
    47641 aaacgatcga tcaattcttc taattcattt agacgttgtt caaacatcga cattgcatct
    47701 tcaatatatg cagcctgtgt catatcaact cctgcttttt tcattacttc cacaggataa
    47761 tcgctgttac ctgcttttag gtaagccaag tagttctcta gtgcctctgg ttcctggttt
    47821 agtattttct tcgccagtgc tgaagcagca gaaaagccag tcgagtattg gaaaacatag
    47881 taattataat aaaaatgcgg tatccgtgac cattcgaatt taatttctgg gtcttcttcg
    47941 actgctggac cataatattt tgcattcaat ttaccataac tatcacttag gtattcacta
    48001 gtcaatggca cacctttttc atcttccgta tgcatgaaat gctcgaattc agcgaattgt
    48061 gtctggcgga aaactgttcc tttaaaccca tccaagtaat ggttgagtac ataagcccgt
    48121 acacgagggt ctttttctgt ttccaataaa tactccgtta ggatattttc attcgttgtc
    48181 gaagcaattt ctgctaaaaa gatggagtag tcgccgtaca cataaggctg gttcgaacga
    48241 gtgaaataac tatgaacact atgtcccatt tcgtggacaa gcgtaaatag ctgatccagc
    48301 gtatcatgcc aattcaataa aatatatgga tttgtgtcat agcttcccga agaataagca
    48361 ccgcttcgtt tccctttatt ttcgacaaca tcgatccaac gttcagagaa tgctttttct
    48421 acgatggcca tgtattcttc acccattggt ttcagtgctt ctaaagcttt ttcttttgct
    48481 tcttcgtacg taaaggtaat tggagcttcc cctaagaccg gtgtataaag atcatacatg
    48541 tgcagttttt ccacttctaa taaacgtttc cgtaattcca tgtatcgatg taacaaaggc
    48601 aaatgcttgt ttaccacgtc taccaaagta tcgtatacac tttcaggaat atgattattg
    48661 ctcaaagacg cttctctggc agagctgtaa ttacggactt tcgctttaaa attatgtcct
    48721 tttatatgtg tgcctaaagt agaagcaaat gtatttctaa attgttcgta aacactgtac
    48781 aatcccttaa atgctgcttc gcgcaccctt cgatccgtgc tttctagcaa ctgaccatac
    48841 acgccatgag ataattggac tatttcacca ttttctcctt caatcgttgg aaaaactaga
    48901 tctgcattat tcaaaacagc aaatgtatca cttgaagcat caaagatttc acctgctcca
    48961 gcaagaagag attcctgctc agcagataag acatgggctc gattatctac tatttgctgg
    49021 atataatggc gatagacttc caattttggt tcttctttga aatattgcca aatctggtca
    49081 tctgacaatt gcaatatttc tggttcaaac caggaaacgg cttcactaac tttcgaaaac
    49141 agactgcttg ctcttgcata aagcgcttgg taatctgtat ttccagtgtc ttgatcgttt
    49201 ttaaggtgcg catatacata aatgacttca gtttggcgat aaaccctcaa tacgaattca
    49261 atcgcattta aaaattgaga agcgccttga tcaagtgttc ctttgtgttt ttcagattgt
    49321 tttaactctt ctgataattc caaatatttc tcatcaaact cttggtcgct tgaaaagatc
    49381 ttggtcaagt cccaagttaa attttcaggc aattcttctc gttttggcaa ctgctttact
    49441 tccatgaaat ccactccttt tcttccctta gctctatcta tcaaaataga tcatagtcta
    49501 acatccacat gtcacagcat ctaacttttc tactcattca tgtgtctgct tccatcaatt
    49561 ttagcataac taaatcttgt tttacataga aaaccgattc ctagcctatt tttatactta
    49621 ctattttttc aaagaagaca agtgtatgca ttcgttaaaa aactgctgat agaccatctc
    49681 ttcatcaatc ataggaaaaa gccattccct tttatgttct tttatttggc aaagccatga
    49741 gttgaaatca ggaactttct ggttttccaa ggcttcttcg tacaacattc gataaagaaa
    49801 aacctgttct tgtaaataaa agaaaaaccg acttttttga tagatccacg atgatagatg
    49861 caaaagatgt ttatgacgca agtagcattg ttcttgtatg gatactgtat tcttttgctt
    49921 gtgaataagt tggcgtgtca gccatttctt cccatcttca ctcacttttt ctattttttt
    49981 catttggttc attttaattt cgttactagt gaatatttct ttaaggtccc tactagaaaa
    50041 agtccagcag ctactgcaaa aggaaatatg ccctgataca gtctctgtaa tatgatgata
    50101 caaataaagc ttttgtttct tgagatcagc tttccagcaa tgcactcctc tttttctatt
    50161 atagtagcag caactttttt ctatcaatga gaattgcgat tgtccaagaa aagaattacc
    50221 aagtatccac catacttggt aactgttatt caaataattt tgggttcttt caataaatct
    50281 ctggtgagac agtctagaac attggatttc tattgctaga ttcgccaaca aaatatccgg
    50341 cctttgccgc aacctcggca aataggcttc caaaaatact ggttctactg actgtcccag
    50401 ccaatccatg aagcattcct ttaactgtaa gtgctctttt gattctcttt cactaaagac
    50461 cgaacagtta tctgattttt gatgggcaaa atgagcacat tttttctttc cctttttcaa
    50521 aatcactctt tttcgacagc cacgacaata atatttcttt cctttttctg catgtttagc
    50581 aaaacagcac ttattatatt cattcattgc aagcagcatt tttctccctc tgaacttatt
    50641 tacgtctaag aatttattta cacgaaaaaa gagggcgtga aaaagctatt ctgcatcaag
    50701 taataagaac agctaaacaa aaatagttcc caatattttt cgtttggttg ggcttgttac
    50761 cgcagatgca agcttttgaa cacatttata cgaaa
//
LOCUS       DACEQH010000016        49881 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.016, whole genome shotgun sequence.
ACCESSION   DACEQH010000016 DACEQH010000000
VERSION     DACEQH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.016"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(84..263)
                     /gene="rpmF"
                     /locus_tag="IW833_000951"
     CDS             complement(84..263)
                     /gene="rpmF"
                     /locus_tag="IW833_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1307085.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(321..890)
                     /locus_tag="IW833_000952"
     CDS             complement(321..890)
                     /locus_tag="IW833_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ1307086.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1076..1978
                     /locus_tag="IW833_000953"
     CDS             1076..1978
                     /locus_tag="IW833_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1307087.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2013..3797)
                     /gene="pyk"
                     /locus_tag="IW833_000954"
     CDS             complement(2013..3797)
                     /gene="pyk"
                     /locus_tag="IW833_000954"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ1307088.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3871..4833)
                     /gene="pfkA"
                     /locus_tag="IW833_000955"
     CDS             complement(3871..4833)
                     /gene="pfkA"
                     /locus_tag="IW833_000955"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ1307089.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5001..8315)
                     /gene="dnaE"
                     /locus_tag="IW833_000956"
     CDS             complement(5001..8315)
                     /gene="dnaE"
                     /locus_tag="IW833_000956"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ1307090.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8546..8731
                     /locus_tag="IW833_000957"
     CDS             8546..8731
                     /locus_tag="IW833_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ1307091.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8793..9989)
                     /locus_tag="IW833_000958"
     CDS             complement(8793..9989)
                     /locus_tag="IW833_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1307092.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10159..11595)
                     /locus_tag="IW833_000959"
     CDS             complement(10159..11595)
                     /locus_tag="IW833_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1307093.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVVTTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11888..12697
                     /locus_tag="IW833_000960"
     CDS             11888..12697
                     /locus_tag="IW833_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1307094.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12883..15036)
                     /locus_tag="IW833_000961"
     CDS             complement(12883..15036)
                     /locus_tag="IW833_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ1307095.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(15330..16178)
                     /locus_tag="IW833_000962"
     CDS             complement(15330..16178)
                     /locus_tag="IW833_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1307096.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(16402..18342)
                     /gene="thrS"
                     /locus_tag="IW833_000963"
     CDS             complement(16402..18342)
                     /gene="thrS"
                     /locus_tag="IW833_000963"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ1307097.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(18679..18858)
                     /locus_tag="IW833_000964"
     CDS             complement(18679..18858)
                     /locus_tag="IW833_000964"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ1307098.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(18905..20728)
                     /gene="cadA"
                     /locus_tag="IW833_000965"
     CDS             complement(18905..20728)
                     /gene="cadA"
                     /locus_tag="IW833_000965"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1307099.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            21006..23285
                     /locus_tag="IW833_000966"
     CDS             21006..23285
                     /locus_tag="IW833_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1307100.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(23378..24025)
                     /locus_tag="IW833_000967"
     CDS             complement(23378..24025)
                     /locus_tag="IW833_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307101.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(24204..24923)
                     /locus_tag="IW833_000968"
     CDS             complement(24204..24923)
                     /locus_tag="IW833_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1307102.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(25054..26205)
                     /locus_tag="IW833_000969"
     CDS             complement(25054..26205)
                     /locus_tag="IW833_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ1307103.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(26422..27162)
                     /locus_tag="IW833_000970"
     CDS             complement(26422..27162)
                     /locus_tag="IW833_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1307104.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(27166..27489)
                     /gene="rsfS"
                     /locus_tag="IW833_000971"
     CDS             complement(27166..27489)
                     /gene="rsfS"
                     /locus_tag="IW833_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ1307105.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(27546..28139)
                     /locus_tag="IW833_000972"
     CDS             complement(27546..28139)
                     /locus_tag="IW833_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1307106.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(28123..28770)
                     /locus_tag="IW833_000973"
     CDS             complement(28123..28770)
                     /locus_tag="IW833_000973"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ1307107.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(28783..29094)
                     /gene="yhbY"
                     /locus_tag="IW833_000974"
     CDS             complement(28783..29094)
                     /gene="yhbY"
                     /locus_tag="IW833_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ1307108.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(29110..30219)
                     /gene="yqeH"
                     /locus_tag="IW833_000975"
     CDS             complement(29110..30219)
                     /gene="yqeH"
                     /locus_tag="IW833_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ1307109.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(30216..30743)
                     /locus_tag="IW833_000976"
     CDS             complement(30216..30743)
                     /locus_tag="IW833_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ1307110.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(30923..31708)
                     /locus_tag="IW833_000977"
     CDS             complement(30923..31708)
                     /locus_tag="IW833_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ1307111.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(31701..32570)
                     /locus_tag="IW833_000978"
     CDS             complement(31701..32570)
                     /locus_tag="IW833_000978"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ1307112.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(32571..33950)
                     /locus_tag="IW833_000979"
     CDS             complement(32571..33950)
                     /locus_tag="IW833_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ1307113.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(33958..34377)
                     /gene="fabZ"
                     /locus_tag="IW833_000980"
     CDS             complement(33958..34377)
                     /gene="fabZ"
                     /locus_tag="IW833_000980"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1307114.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(34402..34878)
                     /gene="accB"
                     /locus_tag="IW833_000981"
     CDS             complement(34402..34878)
                     /gene="accB"
                     /locus_tag="IW833_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ1307115.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(34881..36116)
                     /gene="fabF"
                     /locus_tag="IW833_000982"
     CDS             complement(34881..36116)
                     /gene="fabF"
                     /locus_tag="IW833_000982"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ1307116.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALTTLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(36135..36872)
                     /gene="fabG"
                     /locus_tag="IW833_000983"
     CDS             complement(36135..36872)
                     /gene="fabG"
                     /locus_tag="IW833_000983"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ1307117.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(36884..37801)
                     /gene="fabD"
                     /locus_tag="IW833_000984"
     CDS             complement(36884..37801)
                     /gene="fabD"
                     /locus_tag="IW833_000984"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1307118.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(37908..38132)
                     /locus_tag="IW833_000985"
     CDS             complement(37908..38132)
                     /locus_tag="IW833_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1307119.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(38185..39147)
                     /locus_tag="IW833_000986"
     CDS             complement(38185..39147)
                     /locus_tag="IW833_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ1307120.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(39157..39597)
                     /locus_tag="IW833_000987"
     CDS             complement(39157..39597)
                     /locus_tag="IW833_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1307121.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            39903..40868
                     /locus_tag="IW833_000988"
     CDS             39903..40868
                     /locus_tag="IW833_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1307122.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(40925..41155)
                     /locus_tag="IW833_000989"
     CDS             complement(40925..41155)
                     /locus_tag="IW833_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ1307123.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(41241..42128)
                     /locus_tag="IW833_000990"
     CDS             complement(41241..42128)
                     /locus_tag="IW833_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ1307124.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(42217..43440)
                     /locus_tag="IW833_000991"
     CDS             complement(42217..43440)
                     /locus_tag="IW833_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1307125.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(43457..44536)
                     /locus_tag="IW833_000992"
     CDS             complement(43457..44536)
                     /locus_tag="IW833_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1307126.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(44667..46394)
                     /gene="ptsP"
                     /locus_tag="IW833_000993"
     CDS             complement(44667..46394)
                     /gene="ptsP"
                     /locus_tag="IW833_000993"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ1307127.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(46394..46660)
                     /locus_tag="IW833_000994"
     CDS             complement(46394..46660)
                     /locus_tag="IW833_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ1307128.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            47139..49373
                     /locus_tag="IW833_000995"
     CDS             47139..49373
                     /locus_tag="IW833_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1307129.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            49521..49841
                     /locus_tag="IW833_000996"
     CDS             49521..49841
                     /locus_tag="IW833_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1307130.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        14252 a        10709 c         8725 g        16195 t
ORIGIN      
        1 ttttttattt accgactaca aaaaaataaa cctcaaggct ccatggttag ccttgaggtt
       61 ttacattttc taattaaaat gtattaagct tcttttgaca ttacgtcctt accatcatag
      121 taaccacaat ttgcacatac gtgatggctt tttttcattt caccacagtt tggacattcg
      181 tttaaccctt tgattgtcaa tttgtagtgt gtacgacgtt ttgctttttt agcttttgat
      241 gttcttctag ctggtactgc cattttgcta cacctccttg taaagtcttg tatctaaatt
      301 agacacaata tattccaatc ttacttgtta tcgtcgtctt ttgatttaga atcaaaaagt
      361 tcagatagtt ttgcaagacg aggatcaatt ttattctcgg cttcttccat ttttctgcgc
      421 tggtattctt cttcggaaac aacttcccaa tcattgcctt ttggaagttc tgttgctgct
      481 tcctcttctt ttgtcaatac ccgcattgga atcgccaata agatgttgtc ttcaatcgac
      541 tctgctaaat tcaataatgg accttcaatt agcaaaatct catctgcaga catttgttca
      601 tctctacttt ggaattgttc ttctgtcatg aatacttcgt ctacagaaaa atccatgggc
      661 agttcaacag gttctagtga acgtgttgaa ggaactgtca aagtcgtctg cacagtgtaa
      721 tgaacgatat agcctttctt atcaactgag accaaacctt cgacttccac tggcgcaaca
      781 tcaagtatta aattgtctcg cttcatgaga gcggctttca agtcgagcgt ctcatgaaaa
      841 gttaacggtg tctcctggta ttttctcaat tccaataaag accatttcat ttttcatcac
      901 tcctaagcaa caaaagcaat tatacagggc aaaaagcatg ttgtcaatga aattttcttg
      961 acaccttgca gttcataaaa aaacagaaaa atctttatta gaaagcctct gtttgctatc
     1021 atcctcagaa tcatttacaa taatcattat tccattaaag gagcggatga tcttcatgaa
     1081 cacgaaagat atcgaatact atgtccaaat cgtcaaacaa aaaaatttta cgaaagtggc
     1141 taattatttc cgtgtgagcc aacctactat tacatatgca ttacaacgtc ttgaaaaaga
     1201 gtttgacacc gtacttatta caagagatcg atcgcatcac gaattaatcg tcacgccttc
     1261 tggaaagcag ctgtttctcc atgcacaaac cattctccaa gagatttcta ctgcaagagc
     1321 tgaaatcact cagttgcaaa agaaaaagct tcgttttggc atgcctccta ttatcggcaa
     1381 tgaatacttt cctaaactgt caagttactt tatcaagcat gaattgatga atcaaattga
     1441 gattaccgat ggaggctctc gggacttata cgggatgatc cgacaaggga ggatcgattt
     1501 agctgtatta ggctcaacac agccaatcgt ggatcaatat ttagaaacag agttactttt
     1561 ggacaaacgt ttcatgatcg tcgtgccacc tacacatcct ctttccaagc aggatactgt
     1621 ctctttcaaa gatatagcag atgaacagtt tgtactattg aacgaacatt atgtccaccc
     1681 cgcagcattc actaagcttt ctaaacaaac acatactcat ccaaaagttg tttatcaaag
     1741 caatgacttg acgattttaa agagtatggt ccgagaaaat gtgggtatcg gttttttggc
     1801 agagattgct gtccatcctg aagatcattt agtttgcctc cctctttcag atgaagttca
     1861 gcctcgcttc ttgatttcat tggcaaaacg aacacagcag atcgtcacgc ctcttcaaga
     1921 acaagtagtc caagttatcc atgaattcgt tacagcacaa aaaaacagct cttcgtaaaa
     1981 gaagagctgt ttttattttt tattaagcta aattagatag ctgttgtttc gccacggtaa
     2041 acgatgccgc gacgtgaatc aacggtaatc aattcacctt ctgtaattgt tgttgttgcg
     2101 cctttagcac caacgattac aggaatatct tgtgcaattc ctacaactgc agcgtgagaa
     2161 gtcaatccgc cttcttcaac gataacagca gctgcttttt cgattgcagg catatattct
     2221 ttgtcagttg atggtacaac tagaaccatt ccgtcttttg ctttttttac agcttcttca
     2281 gcagatgaag caataactgc atttgcaacg acagattttt gtccaacgcc ttgagcttct
     2341 aataatttag aaccaatcat ttgaattttc atcacgttag ttgttccgcg ttcaccaact
     2401 ggtacaccag cagtgatcaa gattaaatcg ccttctgatg cgaaacctaa ttcaacagct
     2461 tttttagctg ctagttcaaa catttcatca gttgttgatg gtttttcagc aactgttggg
     2521 taaacgcccc agtttaacat caaaccacgt tttgtgcgtt cgtcaaaagt aacagccaag
     2581 atatctgcat ctggacgata ttttgaaatc atttttgcag tgtatcctga ttcagttgca
     2641 gcaacgattg ttttcacgcc tagatttttt gcagcacgag ctacagataa accgattgtt
     2701 tcagttacat ctgttttatc gaattcgttc aattggaatg tacctaattc tgataatgca
     2761 ttttcagctt cttggtcgat acgtgccatt gttgaaacag cttcaactgg gtagtccccg
     2821 tttgcagatt caccagatag cattgttgca tctgtgccat caaatacagc gttagcaacg
     2881 tcagaagctt ccgcacgtgt tggacgtggg ttttcttgca ttgattctaa catttgtgta
     2941 gcagtgataa ctgggatacc agcagcatta cattttttga taatgcgttt ttgaaccatt
     3001 ggtactagtt cagctgggat ttcaacaccc atatctccgc gggcaatcat aacaccgtca
     3061 gaaactttga tgatttcgtc aatgttgtcg ataccttctt gagattcgat tttagggaag
     3121 atttgaacat gagtcatgtc tttttcttct aagatttcgc ggatatctag aacgtcttgt
     3181 gctttacgta caaaacttgc agcgataaag tcgatatcgt tgtctaaacc aaaacgaata
     3241 tcgtcagcat cttttggagt aatacctggc aaactaatag aaacgcctgg tgcatttacc
     3301 cctttacgtg atcctagcat acctgcattt ttaacttcta cgactaattc acggtttgct
     3361 tcgtcttttt caatgatttg catatcgatc aaaccatcat caaataaaat gtgtccgcca
     3421 acgtgaacat cgtcaaataa tcctgggtaa gtaaccgcga ttttttcttt tgaacctaca
     3481 tgttcaggat ccatagagat gcgtgtttga tctcctactt caaatttgat gtatcctgca
     3541 cgaccaaaat cagcttctgt tgtgccttga acagttgtac ggatttcagc tccttttgta
     3601 tctaaaagaa taccgacatc ttttcctgtc tttttaactg cttcacggac catagtcata
     3661 cgtgataatt gttcttcatg gtcaccatga gaaaaattaa agcggaagac atttgcccca
     3721 gattcaatca attgagaaat aatttctact gagttacttg ctggtcctaa tgtactaacg
     3781 attttggttt ttttcattta aaaaactctc ccattcaatt ttgtttattt aaagctgtac
     3841 acagcttaaa tagcttggtt tctttcactc ttaaaatgaa agtgaattat ttagatcata
     3901 tagtgataag tctggtttat gtttgttgtt ttcaagtgta tcaatgatgt cagacgcaac
     3961 gatttcgttg tcatggatac cgacacataa tccacctttt ccttcttgaa gcaattcgac
     4021 agcgtaagca ccgaatttac ttgccaacac gcggtcgcgt gcgcttggtg ctccaccacg
     4081 gacaacgtgt cccaagatag atacacgtgt atgatagtca ccaaattctg aaagtttctc
     4141 agcgaattca tttccgccca ttacaccttc agccaagatg atcaagcaat gtttcttccc
     4201 gcgatcgcgg ccttcttgga tttttttagc aactgaagcc atatcaaaat catgttctgg
     4261 gatgatgatt tcatccgctc cgccagccac acctgaccat aaagcgatgt ctcctgcatt
     4321 gcgtcccatt acttcgataa cgaatgtacg aacatgagaa gtcgctgtgt cacgaatacg
     4381 gtcaattgat tctagtactg tattgattgc tgtatcaaaa ccgattgtga aatcagtccc
     4441 aggaatatca ttatcgattg ttccaggaat acctactgct gggaaaccgc gttttgtcaa
     4501 agccattgcg ccatgataag agccgtctcc tccgataacc actagacctt cgattccgaa
     4561 ttttttcaat tgttcgattc cttttaattg accttcttca gttgcaaatt ctggataacg
     4621 tgcagaatac aagaatgtac caccacgttg gatcttgtca cctacatctg ccacatcaag
     4681 acgacgaata tctccagcaa ctaaaccggc aaaaccataa ttgatcccgt atacttccag
     4741 tccttcgtaa atacctttac gaacaacggc acgaacagct gcattcattc caggtgcgtc
     4801 tccgccactg gtcaaaattc cgatgcgttt cataataaac attcacctca ttaaaatact
     4861 ttatcctaag tatgacttag gtttacaacc atttttgcgt taaccacgac taacattcta
     4921 acattatttt tctcacttgt cttgatttac ggataaaaaa atgaaaaaaa atgaaagcaa
     4981 gtagtatttt ttaccgtaag ttatttgaaa accacattat cttcgccaag cagataaata
     5041 agttgcccct ttatactctt gctgtcatcc acccaataag catctgacag aactactttt
     5101 cgttgcgttt tttcgtgata cataactact ggtatatatc ctgggtgttt ctgaaaaatc
     5161 acttgtaatt gtttcattat ctcgggatct tcccgttctt ctgtaatacg caaatagcaa
     5221 gtttgtgaag gatactgggc ttctaactgg ttaggatcag caaagctttc tccaattagt
     5281 tgcagttcct gattatattt gctgatttcg ctccgccctt gaacaaagat cactcgatca
     5341 acggcaaaca aattacgata tttacgataa agagttggaa aaactgtgac agaaatttct
     5401 ccactactgt cggttccttc aacaaaggcc atcagttctc ctttttttgt ccgaatttct
     5461 ctaacttcct tgatcatcac taataaattg actggttgat tagggacgat atctgtaatc
     5521 agctgaactt gtttagctaa acgaagacga tcaaaccctt ctgcaggatg acctgacaaa
     5581 taaataccta agtactcctc ttccagcttc aagcgttctt ctagtgtata gtcagcgact
     5641 tcttcttctt ttaatgccat gattcccagc aagtcaagac ttccaccact gtaaacgata
     5701 ttttggattt ttccttcgag atcaagcatc aattgtttcc tgttaggaga taattcatca
     5761 aaaactccta ctgctgtcag tgattgcagt aactctaatt tcagccaacg cttatcgatc
     5821 cgtatgagga attgatccac tgactggaac gggccatttt gttttctttc gtttaatata
     5881 tcttctacaa aatcacgccg aatacctttt atcgctgtca gtccgaatct gatttcttcc
     5941 ttattgacca actcaaaact ggaattactt tgattaatgg acggcgagga aagtttgagt
     6001 ttatttttac gtgcttctgc aatatattct ttcaattttg atgtgttatg ccgaacagaa
     6061 ttcatcaggc tggtaaagaa agcacctgga tgatgggctt ttagatacgc catttggaat
     6121 cccacgaatg aataagcaaa tgcatgtgac cgattgaatc catagtttgc aaatttttcg
     6181 atatattcat atacggtcgc tgctgttttt tccgaatatc cctgttgtaa tgctcctgat
     6241 acaaaatgcg acttttcttc atctaatacg tcttttttct ttttactcac tgcccgtcgc
     6301 agaatatctg actgccctaa tgtaaatcca gccatagtcg aggcgatctg catgatttgt
     6361 tcctgataga cgatcactcc atatgtgttt ttcaaaatcg ggatcagtga atcatctggg
     6421 tagcgaatgg cttcttttcc tttcttcctg gcaatgaagg tatctatatt ttgcataggg
     6481 cctggtcgat acaatgcatt gactgcggca atatcttcga tgttttctgg atctaatctt
     6541 cgtaaaacgt tccggatacc agcagattca aattgaaata ctcctgaggt ttcgcctcgt
     6601 tgaaacaaga gcagcgtttt tgcatcattt agcgggatct cttttaatcg gatctcatac
     6661 ccttctactc gcttaacagc tttcaaagta aaatcaataa tcgataagtt gcgtagtcct
     6721 aaaaagtcca ttttcaaaag accgattgct tctacctcat tcatcgtata ctgggtcaga
     6781 aaagcttcat cagaaccgcg ttggagcgga actagttcaa gtagattctt atcgctgata
     6841 actacacctg ctgcgtgagt cgaaacatgt cttggcaatc cttctaaaat cactgcagtt
     6901 ttgaataaaa gttggttttt ttcgcttgcc tgaactaatt ctactaattg atgcgaagtt
     6961 ttataagctt gggtcaatgt gattttcaat tgattgggta ttgcttttga ccagcggttt
     7021 gcttcacttt gcgataaacc aaacactcta gctgcatctc taagaaccat ttttgctgcc
     7081 attgtgccaa atgttgcgat ttgtgccata tgatattgtc catacttttc tcgaacataa
     7141 ttcaatactt cttcacgtcg attgtcagga atatccaaat cgatatctgg catcgtaaag
     7201 cgttcggggt ttaaaaagcg ttcgaacagc aaatcatatt ctaatggatc cacatctgtg
     7261 atttctaaca cataggctac taatgaaccc gcagcagaac cacgacctgc accggtaacg
     7321 attttgtttc tatgagcaaa agccattaca tcccatacga tcaagaaata atcatcgaat
     7381 cccatttgat ggatgacaga taactcgtag tccaaacgtt cttggtattc ctttgtatag
     7441 tcagtgattc ggcgttccac ttgcgaatag cataactctc ttaaatattc tccagcttgt
     7501 tttccattag gaactggata atgcggaagt aatttctgat ggaactggaa ttcaaatgaa
     7561 atcgattcag ctaaacgctg ggcgcctatt acagcttggg aaagacccgc tttttcgaat
     7621 cgtttgatca tttcttgttc tgaaagaaga tagacatttg ctgttagtgg taaagatgca
     7681 gataaatttt ctaaaggtat ttggcttccc tctttgatat gtttcatcac ttcaagtgca
     7741 aaaagttctt cttgttgtaa actagcgatg ggatgtaatg cacaaagcga ataattttct
     7801 tttatcacat actgatacca ttcaggcaat tgatcaggcg tgttttctgt caagccgata
     7861 taaaaatttc tagtaaaaac attttcaagt aaaaactgcc aatgaccgaa aaagttatcc
     7921 tctttattga aagcctcaga aacagaatga tttgccggaa caacaaacgc tagatggtcg
     7981 gtttctgtca tttctgcaag ttccaatttt tcttggatca tttttcggct ggaaagtttc
     8041 atcagctgct gataacctat ttcatctttt gcgtacgcaa gcaagccata atttttttct
     8101 tcataagtat aactaagatg taatccaatg attggctgta ctccttcttc tacacaaata
     8161 cgatagaatt caggtgcacc gtataagaca tctttatccg ttatggctaa tgaagtgtag
     8221 ccttgttttt ttgcttcttg tacatattct cgaatacgta tcgtactttg taaaagagaa
     8281 taagaagtaa ttgtataaag ttgtgggaaa aacatcttgc attccgcctt ttttttcgtt
     8341 aaaattcact agcgttaaaa ggtttgatcc ttttactttc attaagatag caggcgctga
     8401 agttgattat tccacttcat ttgcttgctt gtaatctgta ggaatgagtt ctttcgcaag
     8461 aactcaaatt ttattgagat ttggtctcgt ttctttacat tcaaagaaaa tctggtaaaa
     8521 ttaagataga aaagaggtgt tctttatgcg atatatcgtt acattattct gggctgtcgt
     8581 tctaggtcaa gtagtcggtt atattggtgc tgcattaaca agcggaacat atgattttac
     8641 tttgacaaca atcatttcat tcattgcggg tgtcattatc cttttgattg gtgcagttgc
     8701 gccaagaaaa gaaacctctg cccattcgta aaaaaacagc caagagcttg agatttctca
     8761 agctcttttt tattttctac tcctcaccac cattataatc tgctgcgaag aactttacga
     8821 aagttgaata aagaaagaag aatatttgac agaacaaaca aacttgtcac aataaaaaca
     8881 gcactgtaat tataagcatg cgcgactgtc gatcctagca tgggacctaa aacttgtcca
     8941 aagttgctaa acatctgatt gtagctatag attcggctga ctccttcttg aggcgtaatc
     9001 ttactgatca gtgtattgac agaaggcatc aaagcgccag ttgaaaagcc taaaataaag
     9061 cgaagtatac ctaattggag cggtgtagtg acgaatgcca taggtaaata acagcaaaat
     9121 gataaaatga gtcctccaat tagaactttt tgattcccaa tcttatcccc tagttttcct
     9181 agtttcggtg aagaaaagac agcggaaaca ccagctatcg aaacaatcaa gccgctgaca
     9241 aaaaggatat tttctgtact tccgctcaat tcacgaatat ataaggtcaa aatagggctg
     9301 atcgtagtga ttcctatctg taaaatcaag gtcgtaatga agagaccgat caaaatagag
     9361 agatgatcca tttttgaaaa aatttctttg gtgcttatca gatctttttt ctcgattgga
     9421 tggaaatctt ctttgactaa gaaaatggtc aataccgttg tgatcatcaa caaagcccct
     9481 gtgatcaaga atacattttc cataccaaac cactgagcca gtgcaccgcc cattgaaggt
     9541 ccgatcagat ttccagcaat cgctccggta gacaatgttc ctaacgccca tccgctcttt
     9601 tctctaggtg cctgtgaagc aatcatcgct gtggcatttg gaatatatcc agacaaaatc
     9661 ccattccaaa accgcatgat caaaagccaa tacgcgtttg gtacaaaagc caaagatccc
     9721 attgttaatg tcatccctgc tgctgcacgg atcatcatga gttttcggcc ttttcgatca
     9781 gctagatttc cccagattgg tgcaaccacg gcagatgcaa aagcagtcac agagattgct
     9841 agtcccgaaa acaattcgat ttgatctttt ggtgttccta gttgctcaac ataaactggt
     9901 atgaatggca taactaaact aatacttgca ccagtgaaaa aacagccaat ccacgcaatc
     9961 actaaatttt ttctccaatt aatctccatc tatcatccct ctttcgtctc ttttatcata
    10021 cctattttcg aataaacaag caacgatctc gccagtaaaa agagaaaaat cccgcaaagg
    10081 tcattttata tgtaacgttc tttttatgta aaacaaaaag agatcagaac aaatactgtt
    10141 ccaatccctt ctctttagtt aaattgttgc tagaatgatg aagttgataa tgaataaaat
    10201 atcaacgatc cacaaagcaa cagaaacttg attgtatttt cctttgatta ctttcacaat
    10261 tgtatagaaa ataaatcctg ctgcaatccc ataggaaatg ctatagcaca atcccataaa
    10321 gatcgatgca aagaaggccg gtaaagcttc ttccatatct agccatttga tgtctgcaaa
    10381 agaagccatc atcattactc caactaaaat caatgctggc gcagttgctt gatttggtac
    10441 aatggcgatc agtggagaga ataaactact taatgcgaat agcactgcca ctactactga
    10501 agtcaaacca gtacgtccac cggccccgat cccagctgca gattcgacat aagttgttgt
    10561 attcgatgta ccaaagatag cccccacaga agtagcgatc gcatcggcaa atagtgcttt
    10621 atccattttt gttttgaacc cacgaccatc ttccaacgcg atctcgtctt cttttgagaa
    10681 aatacctgtg cggcgtcctg taccgataaa tgttccgatc gtatcgaaag tatcagataa
    10741 actgaatgcg atgatcgtca tcaatacttg aggaatcttg gaagaatcac tgaataaaga
    10801 ttgcatgcct tcagaaccaa atgctgcacc gaaagttgtt cctaattctt taaatgaatt
    10861 acctaacgaa ttcgtttgcc aatcaatgga acttaaatca acaacaccca taaaaatacc
    10921 aaggactgtg gtcactacga taccgataag aatagcccca cggacatttt ttacaactaa
    10981 aatggctgtc agtaccaaac caatcacagc caataagata ggagcgttgt caaagttggc
    11041 taacgcaggg acgattccac catttatcgt aacatttgtc gcttttccgc cttcaacgac
    11101 agagctcgaa atagctgact ggtctgctga aaaagataag aatcctgcat ttttgattcc
    11161 gacatacgca acgaaaatac cgatcccgcc accaatcgca tgttgaagac tttcgggaat
    11221 cgcccgaata atcatttttc gaatatttgt gacagtaata aaaatattga ttaatccgca
    11281 aataaacacc attgccaagg cttgttgcca agaataacca agcccaaaaa cgaccgtaaa
    11341 agtgaaaaat gcgttcaatc ccataccagg tgcctgagca tatggtacat ttgcgaacaa
    11401 tcccattacc agcgtaccaa taatcgatgc aatgattgtc gctagaaata ctgcttggaa
    11461 gggcatacct gatgctgaca aaatcgtcgg atttacgaat aagatataac tcatggcaaa
    11521 gaatgtcgtt actcccgcca ttatttcagt tgatacattt gtattgtttt tctttaaacc
    11581 gaaaaacttt tccataaaaa gttccactcc ctgaattttt ttctgatgga aaactataat
    11641 aataaaaaat aagtaaaata gggcaaatcc atcgaacaat ttcaaattat atagacgttt
    11701 cattcgcttt tcaacgtttt tttaacaaaa aaagcgaaaa taatacttaa cgttcgtgtt
    11761 tagcttatgt ttagctattt atatagccat aactgtagtt tttgttagga atagctgatt
    11821 gctcttgtta attacctaaa atatgctaga ctaaaacaaa tgctagttga ggaaggaagg
    11881 acccttattg aataaaaaat taattgccat cgatctggac ggaactaccc tcaatcaaga
    11941 ttcgatgatt acatctaaaa caaaagagac tctaaaaaaa gcaatcaatg ccggtcatca
    12001 tgtcagtatt gctacaggac gcccattccg aatgagccat cagttttacc aacaattaga
    12061 attaacaaca ccgatggtta attttaacgg cgcattagta cacatcccta atcagcactg
    12121 ggatggtgaa agagaaacgt tgatcaatcg tgaaatcgct tttgaaatct tatcgcaaaa
    12181 aaagcagcta aatttagatt ttatcgctgc tgaaaatcgt gatacttttt ttattgacag
    12241 ttttgatttc tttgatgaaa aaatttttgc ttcttctcgt ccaggagaga agaatttgct
    12301 ttctcctaaa aatttgacga caaatcctac atctttgctt gtccgaacag acaaacgttt
    12361 tgcaggagct gtatctgctg aattaacacg tcaatttggc agttatgtcg atgttcggac
    12421 atggggtgga cctactgcca tcttagagat tgtttcaaaa ggaatccaaa aagcaaaagg
    12481 cgtgcaagag attgctaact acctgtctat cgatcaaaaa gacgtcattg cctttggtga
    12541 cgagcataat gatttggaat tgttggatta tgcaggttgg ggagtagcta tggctaacgg
    12601 tactgaccaa ctcaaaggca ttgcaaatga tgtcactcct ttgagcaatc aggaagacgg
    12661 tttagctgtt tatctggaaa aactacttaa actataatag agaattagat tccacaaaaa
    12721 agaggttgtg acaatttttg tcacaacctt ttttgccgag taaacggtgt tcaaaagcca
    12781 gtttctcggt atgaagtcaa atttaagcaa aaatgtgaga agctattttc acaaaatttt
    12841 tcttcatact cgaaactaag cggctttttc acgacctctt ttttattttg ccatgaacat
    12901 atcataatac gcgtccatta tttctttagc aatcgtcaag tttatgtggt cttgttcgtc
    12961 taataagttc ggcaagacaa cactgatggc aatttccgga ttgtctgttg gtccgtatgc
    13021 cacgatatta ctattgactg tcgcaatatc tggatgacct tccgcaaccg tttctgcagt
    13081 ccctgttttt gcggaaaggt ccattttcgc actggctaat ggtttggctg ttgtgtaagg
    13141 atcggtacca tggacaactt ggtggaatcc ttcacggagg atcgccatat tttctgcaga
    13201 aatattgacc gtattttcta ctgttgtgcc aatttctttc ttcacttcac ccaagtttcc
    13261 attttcgtca ttgccataga tcgctttgac taggtgcggc tgtactcgtt tcccgccatt
    13321 agcgactgtt gaagcatatt gagctaattg gattggtgta tatgtgtcaa actgtccaaa
    13381 tgccaaatcg gtaaagttac ctggagcata ccattctttg ccattatccg cattgaactt
    13441 cttcatataa tcaacagaac gtgagatccc aggagattca ttcggcaaat cgatcccagt
    13501 cgttgtacca agaccaaact ctttgaaaga gtctctcaat tgctgataag ctttagcttc
    13561 ttcatctaaa ctaggtagtc ccattccagg tgtatagtct agtcctaaca tctttaacgc
    13621 cactttgatc atatacgtat tagaagacaa ttccgatgct ttgactgcat caagagcaac
    13681 ttgtccgctt cgattaaaga cagagctttt ttctgaagca ccttgcagac gaatcggctc
    13741 atcaatcaaa acttgatttc cgctgatcgc gttacttgcc cagcctgctg tcaaagtacc
    13801 agccttgact acagaccctg gatcaaaggc gctagtaatc gtgcctaaag cattctcggt
    13861 gatttctttg gaaccttgtt catgactgaa accagtcatt gctagcactt ctcctgtctg
    13921 aggattcata gccactgcat aagcaccagg cgaatactgt gctttgccgt tgttgatcaa
    13981 tgtttgataa ttccttttca aaatttcttc tacttttgat tggaattctg cattcataga
    14041 taacattaaa ttagaacctt tttccccaga gaagatttct ttttgattcg aaacatttcc
    14101 ttcattatca agggaaactt catattgggt tttggttcct tgcaggacat cttcatattg
    14161 tttttccaaa taactttgtc ctacacggtc attccgagaa taaccttttg ctaggtattt
    14221 ctctgcttca tctgctggaa gtccttgctt ttcagtcgtc acactgccaa gaatactttt
    14281 cagtgaactt gctgcattat attctcgtgt ccagtctgtg ccagtcgaaa ctcctggaag
    14341 ttcagaagcg cgttctgcca caacagctaa ctcttgatca gtaacaccag agtttttgat
    14401 atagacagtg tttaattcat atgcactatt catccgttta aagatggtag caatttttaa
    14461 ttgctcctcg tttaaattaa tctcatcagc tgtgacttta ttgaccatcg cactatattg
    14521 ttcgctgttc cctaattgct tttcttttgc cgaaagtctt tttgtggctt ctttcaaatg
    14581 tttgggatca gccagccaaa aatctttttt atctctatct gtcagatttt catctaccgg
    14641 aacatcgatc agcgagttca gcttcgttgc gatttcaagc aaatctttag ccgtaacttg
    14701 tgttcctctc gtatatgtga tggcctgatt cgctttattc gtaactaaag cttttcctgt
    14761 ggagtcgtag atcattccgc gaggagtact ttcttggacc gtaatcgtag aagtagactt
    14821 cactcgcgcc tccatctctt cgccgttcac gatctgcaaa taacccaatc tagcaatcaa
    14881 cgcaacaaat aacgtaaata taatgaaaaa aagaaaattt aagcgaaaag gaacatggga
    14941 ccggcgaaac gttttgttct taggttcttg tgcacctttt cttatttttt ctaaccaaga
    15001 agtatttaat atttttttca taaaatcttt catcatttcc tatccttccg cctatttaca
    15061 ttcatgcttc attgtaccca aaaacttctt aataaaaaag aaaaaatggg cggagcaaga
    15121 aattctttac ctaatttcaa gataaaatca actttttgtt tcacagcaat cacttaaagt
    15181 tacatatcca actatttttt tcattaaaaa ataaacttat ttttgaaaat tatgttttct
    15241 taagtcgtga aacactttga tggatcattt atttgtggaa aactacgttt tgttattcta
    15301 aaaaacttat ataaaaaata gtaaggtttt tatttcgaat ccataaaata ttcttggatt
    15361 gcttctcgtg tttttttcac tttttcgcta agcttttcgt ttttagccat atagaaataa
    15421 aaaatgatat cgatcgtagc aaattgggcc aaaagtgaat tagttgccgc acttcgattc
    15481 gtgatttctt ttggtctaga tgtttgtacg agtacatcgg ctatcttgct aagcgggtta
    15541 tttccaaatt gcgtcaacgc aatgatttcg atattcatgc ttttcgccag ctcggctaat
    15601 gcgactacat ctgcacttct acctgagttt gaaacaagcc aaagcacttt ctttttctca
    15661 ttgctgacaa gttgcgggag caagacgtgg atatcttttt caaaaatgat tggcttaccg
    15721 acacgagacc atttttgcag aatatcttct actactaaag aagaggcgcc aaccccatat
    15781 aaaaaaacag tctctgccga ttccaaacac tctacgactt tttggatcgt ttcttcttct
    15841 agaatatcta ctgtttcttt tatcgctact tgtgcattgt taccaagttt gtttttgata
    15901 gagtaaaagg actcatttgc ttctatatca ctaaaacctt catcgattcc agtctgtagt
    15961 tctgttgaaa tcgtgatttt gaagtccgtc agactttgaa aaccgatttt tttggaaaaa
    16021 cgtacaactg tcggcgcact caacccggaa gcttctgcta attcattagc tgtcattgta
    16081 gggaccttct caatattacc gataatgtag ttggccaact ttttttctgc tgaagaataa
    16141 tcttccatca tactttcaat acgactaatt acgctcataa gttcctccac ccttttaatg
    16201 atagtctatg taagtatacc atatcagacc cttattcacg gtcgtatact ggataaaaaa
    16261 caaaaaaaca atccgcccaa cagaatatct tatcccatca atcccacaga ttctattgag
    16321 catctgtgtc tttgaggaat acctgttatc tattgttcgg attgctttct atctactact
    16381 tttttacagg aaatttacct attagttttc tctgctgtaa tttttgactt ctgcttccat
    16441 agcatcaata aagatattca aatcttctac agctgtttct ttacttccat aacgacgtac
    16501 gttcactgtt gcatcttcca attccttgtc tccaacaact aattggtaag ggatcttttg
    16561 tgtttgagag gcacgtatct tatagcccat tttttcatta cgatcatcaa cttctacacg
    16621 caaccccttc atttgcaaac gttctttgat ttcataagca taatcgccat gtgcatcaac
    16681 agaaacaggg atgattgttg cttgtatagg tgccaaccat gttggaaaag cgcctttgta
    16741 tacttctgtt aaataagcga caaaacgttc cattgtagaa acaattccgc ggtggataac
    16801 tactggacga tgcgtatttt ccccgtcttc tccaacatat gtcaaatcaa agcgctctgg
    16861 caataagaag tctaattgta tagttgataa tgtttcttct gtaccaagag ctgttttgac
    16921 ttgaacatct agttttggtc cgtaaaaggc tgcctcgcct tctgcttcaa agtaatctag
    16981 ttctaattca tccatagcgg ctttcagcat tgcttgggct ttttcccaca ttgcgtcatc
    17041 atcaaaatat ttatctgtat tgtttggatc gcgatagctc aagcggaaac gataatctgt
    17101 aatattgaag tcagcatata ctgccaccat caattcaagc gtacgtttaa attcatcttt
    17161 gatttgatca ggacgaacaa atgtatgacc atcattcaac gtcatttctc gtacacgttg
    17221 taaacctgac aaagcacctg atttttcata gcgatgcatc attcctaatt cagcgatacg
    17281 aattggtaat tcacgataag aatggatcgt gtttttgtag accatcatgt gatgagggca
    17341 gttcattggg cgaagaacaa gcatttcgcc atctcccata tccattggcg ggaacatgtc
    17401 ttcttgataa tgatcccagt gtccagatgt tttgtacaac tcaacatctc ccattattgg
    17461 tgtatataca tgttggtaac ctagactaac ttctttgtct acgatataac gttcgatcgt
    17521 gcgtcggatt gttgcccctt ttggaagcca gaatggcaaa ccagaaccta cttctgggga
    17581 aaccatgaac aagtcaagtt cttttcctag tttacgatgg tctctttctt ttgcttcttc
    17641 acggagacgg atatattctt tcaaagcttt cttgtcaaaa aaggctgtcc cgtatacacg
    17701 ttgcatcatt tgattgtttg agtttccacg ccaataagca cctgccactg ataataattt
    17761 aaatacttgt atacggccag tggatggaac atgtggtcca cgacacaaat cgatgaattc
    17821 gccttgttga taagcagtaa tcacttcttc ttctggcaat tcactaatca gttctacttt
    17881 gtacggatca cttgcaaaaa tttctaatgc ttcctgtttt gaaagaacac ggcgttcgat
    17941 cggcagattt tctttgacga ttttcatcat ttctgcttcg attgccggca aatcttctgc
    18001 agttacttgg ttttgtccat tatctgtatc gtagtaaaac cctgaatcga tagctggtcc
    18061 aacgccaaag tggatatttg gatagaggcg acgcatggct tgggccatca aatgagctgc
    18121 tgaatgacgg actaagccaa gtgcatcttc atggtctggt gtgatgatct caatacttgc
    18181 atcttcctcg attggacgag tcaaatcaac gagttttccg ttcactttac cagctaaagc
    18241 tttttttgct aagctttttg aaatgctttc agcaattgcc aatgttgttg ttccagactc
    18301 aaattctttg actgcaccat ctggaaaagt aatttttatc ataatagttt cctccttgaa
    18361 atgttttgat tgatcagatc atcaagctga aaaacaaaaa aatcctagca tctcttgaaa
    18421 agagatacta ggacgaaaag ttcgtggttc cacctaatgt tcaaacgcaa atgcgttcct
    18481 caagcgttgt aacggtccga ccgcccttgc ttcaacaagg gtttcgaaag tggtcatcct
    18541 ttggttttct cctggaaaag tttcagccta gcttttcctc tcttatcgaa gactctcaaa
    18601 ttcagttggc tttctcatca atcaattatg tgtttattta accactaata aatagaaaaa
    18661 tcaagtggga aactaaactt attttttcca ttccccattt acaccgtatg ttcctttttt
    18721 catctcattg atgatgacat ggatgtgttc ttttggtgcc tgcgtgtttt tatgaacagc
    18781 ttccgtgata tctttcatca tattttctaa ttgttccgga ctgcgacctt ctactaattc
    18841 gacatgtacg aatggcataa tgctctctcc tctttctgat cgttcttttt tcaactcgat
    18901 tctatcatac tatgtttgtc ttgggtactg aatcaggaaa aatggctgga tctttttttg
    18961 ctctcaaggc actcaaaata gagaccgtat ccacgacttc ttgcaaaaca gctccgaata
    19021 atgcggggat tagtcccgta cttgcaatta acatcaaaaa aatacaaata aagatgccaa
    19081 tcagtacaga ctgtttcgct attctcatcg tatctcttga aatcactact gcttttgcta
    19141 cacgcaagag atcatctttc aagatgaccg catctgcact ttcactagca gctgtagaac
    19201 catgagcccc cattgcaata ccgatatctg cagcagcaag tgctggtgcg tcattcacgc
    19261 cgtctcctac catcgcaaca ggtctttgat ctttagggat actttctaaa atggtcagtt
    19321 tatcttgagg caagctacgg gcatgtacag aatctaattg gactttttct gcaatcgttt
    19381 gagcaatggc agatccatcc ccggtgatca tcatcaaatt tttcacatca agttttctta
    19441 gctccttgat tgtctttgct gcttctgggc gaatctgatc cgaaaatagg atcgttcctg
    19501 caaaagatcg atcaattgtt acatagattg ctgtattgga cgtttctatt tcttcagctg
    19561 aaacaaaaga ggcacgtcca atctttactt catgattatt gactactgct tggattcctt
    19621 gaccggtcac ttctttcagt tgactaacag gatacagttg gctgtttttc acgttgttca
    19681 ctaacgaacg agctaagata tggctagatt cctgttctgc actagctgca tacagaagta
    19741 gctcttcttt ttttattcca ttgactggct gaatcgcctc tacttccaat atccctttgg
    19801 taagcgttcc tgttttatca aaagcaatcg tttttgtacg tgcgagcttt tcaatcgttg
    19861 ttccagtttt tacaacgatc ccgtttttac ttgaacggct cattcctgca actaaagcaa
    19921 tgggcgcagc tagaatcagt ggacaaggtg atgccactac cagtacctca gcaaatcgta
    19981 aagggtcttt tgagaaccac caagaaatac caccgatcaa ataagcaatc aacgtgaaag
    20041 ggactgcata acgatcagct aaacggacaa atcgggcagg cttttctttt gattcttcga
    20101 ctagtttaat aatcatttga tactggctgt ctgcggcacg tttttcagcg atcatttgga
    20161 tcgatccgtc tccatttaaa gaccctgaca taatctgatc tccaggtttc ttggtttccg
    20221 ggcgggactc acctgtcaaa gaagattcat cgaccgaaga ttctcctttg gtcactcgac
    20281 catctgcggg tacaagttcg ccaggcttga ctagtaactg gtcatcaata tttatttcat
    20341 cgattactat atcctgcaat tttccttctt tgaatcgatg ggcaattctt ggggtgttat
    20401 ctaacaaaga ctgcagttct ttacccgctc gtttattcgc gtaatcctct agactatctc
    20461 cgcccgtaag catgatcaat atcatcaaac tagcccagta ttctttgacg gctaatgttg
    20521 caaggattgc ggtaatcgct aatacatcta cgccatatct tcctgaacgt aacgtcttga
    20581 tcatttccaa aaacatcaag aaggcgagaa tccctccagt aaatgcgaca atcgcaaatg
    20641 caagggccgg ctggtgaaaa acaaattcac ttaataaagc tatcccacca ataagcaaag
    20701 taagtaaaaa ttttttgaaa tgtgtcattg ttttttctcc tcttttatgt gacttagaga
    20761 ggttagtaat aaatttccag ctgcctcttt catcttaagc atagtccttg actagcactt
    20821 tttaaaacaa taggccttaa ttgaaaacaa ctatcactat cagcgtttcc aaaattcatc
    20881 agttctacaa acaaaaatat tcagcaagga ttactttttc gattcatcag ttaaaaagta
    20941 ccttttttcg aaaaactatg ctatactttt aattcgttat ttacggcgga aggagcaaac
    21001 atgcgatgaa tgagaaacaa ctagaacaaa cgcacgtgga tgaaacgatc gaactaatcc
    21061 agatcgaaca aaatatgctg gatacaaaac aacaagaatt gactcaggaa atgaatcatt
    21121 cgacaaaaga cacagctaat cacaagattc gagctggttc caatgaagct ttttatgaat
    21181 ctgctgttga atatcgccaa catgagcagg aattgctttt gaaataccat actcttgaag
    21241 cacagcaaaa acgcctgcaa acgttgtctg tcatgaaaga tagtccttat tttgcccgta
    21301 tcgactttaa agaagaccgt gaaacagaaa cattatatct aggtatcgct tctttaagag
    21361 atctggcaga agaaacaatc gttattgact ggcgcgcacc aattgccaat ctttattatg
    21421 aaggtgagat tggtcccgca ttttatgaaa ctgaaacaga tagaatagat gtcgatctgc
    21481 agttgaaacg acaatttaaa atccaagaag gacgtatttt atctatggta gatacgtcgg
    21541 aagtaatcaa tgatgacttt ttgctagaga tattggatga atcatcaagc aaccatatga
    21601 aaaacatcgt ctctacgata caaaaagcac aaaatgcaat cattcgggac acaacaagta
    21661 aagtaatgct gattgaagga atcgccggaa gtggaaagac atccgctttg cttcaacgta
    21721 ttgcttttct gttgtatcat aatcgcaagt ggctcgaggc tgaaaacgtt cttctatttt
    21781 cacccaacca tttgttttct gattacatct cgactgtatt gccttcatta ggtgaaagcg
    21841 gcgtgcctac acagacattc aaaaattatt tggtgcagct gctgccagag ttttctttac
    21901 tagatgaaca gcaacaagaa agtggttttt tgctagggga aaaagatccg atacaagtga
    21961 tgaaaagcgg attgacctta gtcgatcaga ttgatcgcta tatccaaggc atcacaaact
    22021 acggtccctt gttccgtgat atgaaaataa atggccgtac gatcctatca aaggaatcga
    22081 ttcgccaatg gtataaagaa acaaatgaac aattaccttt acatcataga ctttctctat
    22141 tacaaacgaa gctcctgaaa aaattaggcg gtctgcaaaa agacgaaaca cgtcagcaat
    22201 gggtcaaaga tctggcggaa gaacagctcc aagaactata cgcaacagat ccaaatcttg
    22261 aatatacaga acaaaaggag cgttcattac gtaaaaaact ggccaaccaa atcgtacgta
    22321 aaagattccg tcgaatccat cgagggatca tgaattatca atttgtcaat ataacgaaac
    22381 aatatctcca ctttctacaa actgtgccac tttctcttct aacacgacat aaaatcagcg
    22441 agaccaggtg gcaggaacat ttgacaactg ttcgtacaca cttgcgtcaa agagaattgc
    22501 gtcaagagga tgctgtacta ttctttttac tgatgcgccg aacatatcca gtgacggtta
    22561 cacacaaagc tcgctatatc tttattgatg aaatgcagga tttcgcacca tcacaagtgg
    22621 cattgctgca agccttgttc ccaaaagcaa atttgacttt ctgtggagat ctaaatcaaa
    22681 atgtctttgg aaatgaaacg atcgttggat cattggaaga ccttttcgtg gaccgtgaag
    22741 tcactcgttt ccaattaacg acaagctatc gctccacaaa agaaatcact gattttgcta
    22801 atcattttat tgtgaaagaa catcaagtag aaacaacagc aagaaaagga gaccttcctt
    22861 tgctcatcca aggaacaaca gatgatatca aattgaattg gttagcagat accattcgaa
    22921 cgactcaggg aaaagcccgg tattggcgta cagcaatcat agctaaaaca caagaagaat
    22981 gcgaagctct ttatgctcaa ctgccagacg agttgaaaca acaagtccag ctgattttaa
    23041 atgagagtga ctttatgaag cgttccatta ttttacttcc agccttttta gcaaaaggtc
    23101 tggagtttga ccgagttttc ttgtgggatg tagatgcaga acatttttct tctcagcaag
    23161 acaaattgat cctttacacc atgtgtacac gtgcgatgca tgaattgacc ctttttacct
    23221 ccaataatga aagtcctttg ttaaacaata tggatatttc atcttatgag aaaaaagtac
    23281 tgtaagtaaa aagacaactg ctatcattaa tgttagcagt tgtcttttta tttacaataa
    23341 taattgcaga aaaagtaatt caatcattta aggtcattta ttctatatat aaatccaaat
    23401 ctgtttcagg atattttaat ttgacaagcg aaataatcat tttcagttct tccatgtttt
    23461 ctggatattc tgggtcaaat accttgctat gacggccaat cccgccattg tcgatcaatc
    23521 ggtaaattcg attccattca atgaattggt caattgattg gaaattgctg attggatagc
    23581 cgccacaatc caaaatctct gatacacgaa atgctaggta tgcactatcg atcactttat
    23641 tttttttcgt gtctatcttc tcatttcttt cgataagaat tttttcagga cgatgtttca
    23701 agctccactc taatagtaga aagaaagcct ccatccgata ctcttcttca aataaccgaa
    23761 aggctgaatc agaacgacga aaaagcagcg gaatcagata attttgtcta taaatcagca
    23821 ttggtgcgcg gtccaccata cactctaaat aacggttcag ataccctgta ccgacatagt
    23881 ttcctaatcg atagccttca acgccgaatc ttgcgtattt ttgcgctaat tttgaacaat
    23941 cagttacgtt cacagctaac tcttctactt tgcggtaacc gcttaagata cgttcaaaag
    24001 gttctaaagt gatttttgct tccatattct tccccgtcca tcttccacat acttcatatc
    24061 atttcacatg atacttatgc tctcatcata ccacaggttg aattagaaaa gaaaataaat
    24121 cacacaaaaa agcaagacaa tctgttagtg agattgtctt gcttttcgaa aaagactgaa
    24181 tacttttcgt taaaactggt tgcttagtct tctaatgcca cattatggaa cacatgctgt
    24241 acatcttcta gatcttccag tgcatcaatc attttttcaa atgtttcttg atcttctgga
    24301 gatagtgtta cctcgttttg agggatcatt tggatctcag cgattgaaaa atcagtgatt
    24361 cctttttctt ttaatgcttc ttgtatacta tggaaatccg ctacttcacc gtaaacgatg
    24421 atttgtcctt cttcttccat tacatcgcga acatcgatgt ctttttccat taggtattca
    24481 aggatctcgt cagcgtcttc tcctgcaaaa gcaaagattg ctgtattgtc aaacatgtaa
    24541 gaaaccgctc cggaaacacc catgttcccg ccatttttcc caaatgctgc ccgtacgtct
    24601 gctgctgttc gattcacatt gttcgtcaat gtatcaacaa tcaccattga gccgcttggt
    24661 ccaaatcctt catagcgcaa ttcagagtat gtttcatctc ctgcaccttt tgctttttcg
    24721 attgctcggt caataatatg ttttggtaca ttataagttt tagcgcgatc aatgacgaaa
    24781 cgtagttttt ggtttgcatg cggatcagga tcacctgatt ttgctgccgc gtagatttca
    24841 atcccaaatt ttgcataaat ccggctatta ttagcatctt tagcagtttt ctttgctacg
    24901 atatttgccc atttacgtcc catattttca cttcgctttc tgatttgata gtttcataac
    24961 gcaaaaacgt cctgttccat tatacttgtg aatcacgatt ctgtgaagct ttttttcttt
    25021 cttccttcat tcttctcatt tttagtcaat tatctatact cggattggga tttgcccgaa
    25081 attctgttcc ggaatattgc ctcccaattg atagatttga tcacttctaa ctaacagttc
    25141 cgcttcttgt gcctcttttt ttccgattcg ggaataaata gaaatctctt ttttttcttt
    25201 taagaatctt cttccagcat cggtaaatcc aagtagtcga agataatttt tattttgttg
    25261 gacaggaatc tcttgctttt tgatattcag caagacataa caagataatc gttggatccg
    25321 tgtccatgta tatctttttg tttttacctt tgacacaaaa tcctcaaaac tatcagcagt
    25381 tgcagctgct gttttcatgc gcgattctat tccttcggtc atctgataga tatctgtcag
    25441 ttccagcaaa gtagaagata acaaccgata acgcaaatac ggccaataag attcccaagt
    25501 ctgcagcgaa caagttgaaa gatcttcttc tgtctgaggc ggcacaagtc cagctattct
    25561 ttttccttct tctaacgcat ggcgaatggc tgttgcactg gctatcctct gatctgtgat
    25621 gattttttca tgatagcctg cacttttacg tcggatcgga tgtaattcca tagggtggac
    25681 atacttggca ttttcctttg cataacttaa tcccaagata tgatttggcg aagaaaaatc
    25741 aagcgtaata tcgggataca gactgcgaaa aacctcagtc attttttgcg gataactata
    25801 cgtttgatca gacagttctt gaaatgcctc ttctatccta tcttgattct cttggacaaa
    25861 gcgtccgaaa ctgctgtaat caaacgaagt ctcttcttct gttccaaaac acaaactgtc
    25921 acagtccagt gtttgcaaga tttttactgc acctttagca aaatagtcag ctgactgaac
    25981 cgcccattcg acgggaagct ccactactaa atccacacca ttttgtaaag cttcattcgc
    26041 gcgatgccat ttatcgatga tagccggttc gcctctttgc aaaaaattcc cgctcattac
    26101 agcaatgacg acctctgctc ctgttatttt tcgtgccatt tccacatgat agcgatgtcc
    26161 attgtgaaaa ggattgtact ctacgataat cccgcagctt ttcatgcgca tcctccttca
    26221 tagttatcga taaaaaagaa atagagacaa gtaaaaaaag gcaaaaatcg gaaaaaatac
    26281 ccgaactata taaactattc tcgcaaacac aatacttctg cgacgaaaag ctgcactgca
    26341 agagcaatgc ctatatcgtt cggatatata atttatgttg aagatttatt cttttcttgt
    26401 taactcgagc aggcaaaacc gctagtcttt gtatgctaca aagaaccagc gctttgattt
    26461 ctctgatgct gcttcgtctg taaaatcaga aaatgcttcg actttcgaaa agccgctgtt
    26521 ttccagcatt cttaaataac tttccaatgg ataggtacgc tcttgatgta gttcatcttc
    26581 acgaataaat cgaccagacc cttcttgttc ttcaacgaaa aaggtaagaa aatgttcgat
    26641 gctgtgtttc tcgcttcctg gataactatc ccacaaaaaa gcaaaattat ccccttgata
    26701 atgatagcta tattctggaa aaacctcatc tacttgatag gtggagtgta cgtcaaataa
    26761 aaatcggcca ttttcttcta acgcttgata gacttcatca aacacttgct gtacctcttg
    26821 tctatttttc atatagcaca gcgaatcaga aaaacaggta attgcctgat attgtccgat
    26881 ttctgataaa tcgagcatat tgccttccac aaattggatc gatgcgtctt cttcatatgc
    26941 tcgctggctg gcaatcatca acatctcttc tgaaaggtct aaccctgtca ctttatatcc
    27001 atcttttgca aaggcaaccg ctaatgctcc agtaccgcat gccagttcca aaatattttt
    27061 tgtgtcttct tgtaaatgac gtttcgaaaa agctaaccat tgctgataaa gtgtttcgtc
    27121 catcacttca tcgtaaatga aagcaaatgt ttcataagcc atatgttaat ccaaccattc
    27181 actaagatcg actagcggag catctgacca tagtttttcc agattataga aatttcgttc
    27241 tggcgcatgg aaaacatgta cgatcacatc tccaagatcg atcaataccc attttccgcc
    27301 ttcttttcct tcaatacgtt ttacttcata tttgttttct tcttctttat caatgatttc
    27361 ttctgtaatt gcgttgattt gacgttcgct atttgctgaa cagatcatga aataatctgc
    27421 caacaaggaa acatttcgaa cgtctaaagc gataatgtct tctgcccgtt ttgaatctgc
    27481 tgcttcaatc gcaattttta attgttcttt actatctatg atgatttcct cctaatatga
    27541 gcagcttatt tttttgctac ccagtaatta tatgtttcga ttgtttttgg ataaatctgt
    27601 tgttcttgcg caagcaaatg ggccaatgta tgttttgttt caaatgctac agcttcgtct
    27661 agatcagccc atgcaatggc tcgtgcatct tgtaccccag gaaaatctcg acctggttca
    27721 atatagtctg ctacataaat gattttgtca aggagactca tcttagcagc cccagtcgta
    27781 tgcacccgga ttgcatgaag aatctctgca tcggtgattc ctaattcccg ctctacaaac
    27841 gaagcgccaa caagaccatg ccagattgca ttattataat tcaataactc cggatcaaaa
    27901 ccgtctctta cgattaccat tttgaactcc tcatctggtc gttcttttgc ataatcatgt
    27961 gtcaatgcag caatactagc tttttcagga gaagcaccgt attttttggc aagagccacg
    28021 gctgtttctt ctactccaag cacatgcttg aagcgttgtt cgctcattct catctgtatt
    28081 ttttgcatca ggttttctct agaataagca gtatattcat tactatagtc catattcata
    28141 caacccttca ttttgaatat aatcaattac tttatctgga acaaggtagc ggatagaaca
    28201 gccttctttg atcttttgac gtattttcgt ggaactgatg tcgatctcag gtacatctac
    28261 ccaaattaca ggatatggag tatccgttgt gtatcctggt cgacgtatac ccacaaagtt
    28321 gaccatagaa gtcagctcat cgattttata ccattttggc aaatattcta ccatatcccc
    28381 accgataata aagtaataat ctgtatccgg attgttttgc gtcaattcct tcatagtatc
    28441 ataggtgtaa cttttcccac cacgagctaa ttcaatcgtt tcaatctgca aaaaaggatt
    28501 gtcttccacc gctaattcca acatgttgag tcgatgtttt gcatcaatcg tctgtttctc
    28561 atcaacatga ggtggctgat aagaaggcat caaaaatacg cggtcaagac ctaaattccg
    28621 gccagcttgt tcagccatca ctaaatgtgc aaggtgaacg ggattgaatg ttccgcccaa
    28681 gatccctact tgctttttct tctctaagaa ttgaggcatt tcttcaggaa ataattgtga
    28741 acgaacaaat gtcttagctt gcgtattcat catggtcact ccttatattg cttttacttc
    28801 ttttgatagc ctttgatact tttcttttgt ggaaggtttg tataatacaa taacacgtcc
    28861 aatgatttgg acgatttcgc aatctatttc tttttctaat acttgagctg cttcttctgc
    28921 aatctcatct gtattttgaa gtaacgatac tttgatcagt tctctttttt ctaaagcttc
    28981 ttcaatttga acgatcattg ctgaattgat cccacctttt ccgatttgaa aaatcggctg
    29041 aagatgatgc gctttgctac gtaaaaaacg cttttgtttt cctcttaaac tcataatttt
    29101 ccttcttcct taaatcaatg ctttgcgttt caatacatct acaccctttg gcgcccagcc
    29161 cgcaattact actggcgctg ttacagtgat ccagcctaga ccggcaaaaa cgatgtctgt
    29221 tttttctttc acagaaaact caaaacgaac aagctccgga aatgcctcga cttcatcagg
    29281 acgaggaggc tgtaataatc cacctacatg tttttgataa aattcgtctg ctttttccag
    29341 tttcgttcga tgaatattta catcatttga cacataagca ataaatgaag cacgattgcc
    29401 gctgataaaa tcaaatcttg caagtccgcc caagaaaagt gtttgccctt cattcagctg
    29461 ataaacttta ggtttgattt cttttaacgg tgcaatgatc cgcaaatctt ttttacctaa
    29521 gtagtgcgcc atttgatggc gatggataat tcccggtgta tcaatcaaaa aatgcccgtc
    29581 atctaaagga atctcgattt tatccaaggt cgttcctgga aactgcgaag ttgtgattac
    29641 atcttgtact cccgctgtat ttttgatgat ttgattgata agcgtcgatt ttccgacatt
    29701 tgttactccg acgatataga catcccgtcc atcgcggtat tcttcgattt tttctaatag
    29761 ttccgtcatc tcatgagatt tcttagcact agttaataat acatctactg gacgaattcc
    29821 tgcttcgtaa gcacgttcct tcatccattg tgttaattta gtttttttca atgatttagg
    29881 taatatatcc actttgttcc caactaaaag tactggattg tcaccgacaa aacggtgtag
    29941 tcctgggatt agtgaaccat tgaagtcaaa aatatccacc acattgacga tcaatgcatc
    30001 tttttgccct aattcattca gtaagcgcaa aaagtcgtca tccgtcaatg agacatcttg
    30061 gatctcatta taatggcgta atctgaaaca acgctgacaa tagacttccc cggaagctaa
    30121 acctttttcc aaagccgttt gcggcgtgta tcctaattca ccgggtttgt ttgtttgtat
    30181 aacggcacca cagccgatac attgtaattc ttctgtcatt cgattccacc tcgccagccc
    30241 atatcagggt gttttgctaa tagataggcc ataattcttt tttcccaaaa acgattaaat
    30301 tgtgtcttcc aactatctgt tgcgataatg ggttttacaa ggatgctacg tatccctgca
    30361 cgattggctc cacgtatatc agtcataatc tgatcgccta ccattactat ctcatcatct
    30421 gctaaatcca attgtttctt tgctatgttg atccctcttg ctaaaggttt cattgctcgt
    30481 gcaacataca aaagatcaaa tttttcaatc gctcgcgcga cacgttttga attattgttt
    30541 gaaacaacaa caacagggat tcctgcattt ttcatttcta aaatccaagt aagcaactct
    30601 tctgttccat ctggattatt ccatgcaatc aacgtattgt ctaaatcggt caatactgct
    30661 ttgatgccta gattctttaa ttgggcaggt gtaatctgat agatggcttc taccatccat
    30721 gttggtttgt aatttgaaaa catgctgtct cctcatctgc aaaaaatgaa ggagcacata
    30781 aagtactcct caaccccgta ttatactgca tatcagcaaa aaaaactacc tctatactcg
    30841 ttcgtctttc agaaaaagca tttctttaca aaatgaccaa aaataaagat tgatcacaga
    30901 aaaatttaga aacaaaaaac aactaatatt ggcggaacct cttgtatcgt ttttccagta
    30961 atttatgctg atctaatcga acaagttccg tcaatctttc aatcaacgct tttttcataa
    31021 tgtgtgcgac tttatatcgt ggcaactgct cgccgttgaa cgtttcagga ataactcgat
    31081 caacgattcc taactcgcgt agttctggcg cagtaatttt catcaactca gctgcttctg
    31141 cggcgcgact accgtctttc cacaaaatcg acgcaaatcc ttctggagaa agcacagcat
    31201 aaatcgtttc ttccagcatc cagacttcat ccgctaaacc tagagcaagg gcaccgccac
    31261 ttccaccttc accaatgatg atggcgatga tcggtacctt taaatcagac atttcgagca
    31321 gatttctggc aatagcctct ccttcgccgc gctcttctgc tcctacaccg caatatgcac
    31381 ctgctgtatt gatcaaggta atcaccggtc tattgaactt ttccgcttgt ttcatcagtc
    31441 gtaaagcttt tcgatagcct tcaggatggg gagaaccaaa atttctgcgg atattttcct
    31501 gcagatcccg tcctttttga ataccgatca ctgttaccgg tttgccatct aatcgagcga
    31561 ttccaccaac aatcgcttcg tcatctgcaa ataaacgatc accatggcat tcctcgaaag
    31621 aatcgaagat ctcttcaaaa aaatccaagc ttgtccatcg ttctttagct cttgctaact
    31681 gaacgacatc atgtgctgtc ttatccacga tctatccatc ctttctgtgt atgcaattca
    31741 agaagcgtat ggattttctg tttcaattcc atgcggggaa caatctgatc gacaaaacca
    31801 tgagataata agaattcagc cttttggaaa tcttctggaa gttcctgctt gatcgtctgt
    31861 tcgatgacgc gtcttccagc aaaaccgatc aagctttggg gttctgctag aataatatcg
    31921 ccttccattg caaaactggc agttactcct ccggtcgtcg ggtccgttaa aacagttaga
    31981 taaaaaagac cttcattgct atgccgtttg acagcagcag agatttttgc catctgcatt
    32041 agagaaaaga ttccttcttg catccgtgcg ccacctgaag ctgtgaacaa gacgactgga
    32101 agactttcct ttgttgctcg ttcgaataat ctggtgatct tttctccgac aatcgttccc
    32161 atacttccca tgataaaatt aggatccatc actccaataa tgaagggaat cccctgaatg
    32221 gttgctttac cagtgaggac tgcttcatga agtcctgttt tatcttgcat ttttttaatt
    32281 ttttccgtat attcgggaaa tgctagcgga tctttggtta caagatccgt atcccattct
    32341 tcaaaacttt tttcatcaat aatcaaagct agacgctgcc atgcaccgat tcgaaaagcg
    32401 tatccgcaat gagggcatac tttttcttca ccgatgtctt tcgtataaag gatatgtttg
    32461 caatttggac actttgccca catgttatca ggaacagatg gcgagttagc aggtttgctc
    32521 tctctattag gattgatccg aatatatttc tttttcttaa acagagccat ctatgcgcct
    32581 cctatctccg tttctggtgt ccaattcggt aaaaattctt cttgtaaaaa tgctgtgctg
    32641 taatcacctg ctatgactgc aggatgacta atcaaatcca tctgaaattc tgcgttcgtg
    32701 atgattcctt ctgtgataaa ttcacttaag gctcgctgca tcttcattaa tgcttcaaaa
    32761 cgcgtatttc catggacgat tatttttgca atcattgaat cataatatgg cgggatcgta
    32821 tatcccgggt aaactgcact gtctactcgc agtcccatcc cacctgctgg aagcagtaag
    32881 ttttggatct tccctggaga aggagcaaaa tggaaagcag gattttctgc attaatccgg
    32941 cattcgattg cgtgtccttg gaatactact tcttcttgac acacattcaa aggctcaccg
    33001 gctgctatct ccacctgttt ttttactaaa tcgattcctg tgaccatttc tgttactgga
    33061 tgttccactt ggattcgagt attcatttcc atgaaataaa aatcgccttc ttcatccatc
    33121 agaaactcga tcgttccggc attttcataa ttgactgctt tagctgctcg aacagcagcg
    33181 tctcctagtg cttctctttt tgtttgtgag atcactacag aaggagattc ttccagtact
    33241 ttttgattgt tgcgttgtaa agaacaatct cgctcgccta aatgaatgac atgaccaaat
    33301 cgatcaccta agatctgaac ttctatatgg cgagcaggat aaatgatttt ttccaaatac
    33361 atatcatcat ttccaaatgc cgctgctgct tcttgctgag ctgaagaaaa atgctgtggg
    33421 agctcttctt ttgattgaac tttccgaatg cctttcccgc caccacctgc agctgctttc
    33481 agcatcactg gataaccaat tctgtctgct atttccaatg cttcttctac tgtagataat
    33541 acaccagtac ttcctggaat gactgggaca tttgcttctt gcatcaattg acgcgcattg
    33601 atcttatttc ccattgcatc gatcgttgca cttttaggac caataaatgt gatattgcac
    33661 tcttcacaca tggaggcaaa tcggctattt tcagataaaa aaccaaatcc tggatgaatt
    33721 gcttcggctt ttgtcacgat tgcagcgctc agtacttcct gaacattcag atatgaatca
    33781 acggcttttg ccgggccaat gcagattgct tcatctgcta actgtgtatg aagtgcttcg
    33841 gcatctgctt ctgaatatat cgctacggtt tggatcccta gttcgcggca tgcacgaatg
    33901 atacgtacag cgatttcacc gcggttagct atcaatactt tagaaaacat atttcaccta
    33961 tccaatcata aaggttaatt ccgcttcggc tactttttta ccgtctactt tagcgattcc
    34021 tttgcctatt ccggctgaag ctttcacttt caatatctca acttcaaggt ataaggtatc
    34081 tcccggtgta actttcttac ggaattttgc tttgtccaat ccgccgaaat aagccgtctt
    34141 tcccttgaat tgttcaagag aaagtaatgc aactgctcct gcttgagcca ttgcttcaac
    34201 aatcaatacc cctggcataa caggttcttc tggaaaatgt ccttgaaaaa agggctcatt
    34261 gatcgttaca tttttcttag caacgatccg ttctccttca accatttctt ccactcgatc
    34321 aatcaaaagc attggataac gatgtggaat gatttctttt atttcttgta tgttcattgt
    34381 tttccctctt tcctgctttg cttatttttg ctgaatccta aagagtggct gtccatattc
    34441 gacaactgac tcgttttcta caaggatttc tgtgatcact ccgtcggcct cactagttat
    34501 ctcattcatc agtttcatcg cttcaataat gcagataact tctcctttgg caacttgatc
    34561 acctactttt ttgaaagctg gtttgtcggg cgctggttgc agataaacga ttcccacgat
    34621 tggcgaaaca acctcgattc catcaaatac tgccgattct ttttctttca ttatttctgg
    34681 agcagttgtt gcttgttcag atatgacatt gactggctcc gaaactggca acgtttcgtt
    34741 tgtttgaacc atattcatgc ctcgacttgt tttattttta ttcatataca attcaaattg
    34801 accttcacgc aaatcgaatt ccgttagcga ggattgatca aattgactga ctaattctct
    34861 gatttcattg atatccaaaa ttattcctcc cagcgtttca tgcacagaac cgcattatgt
    34921 ccgccaaacc ctaatgaatt actcattgca taagtaaaac ttgcaggttt gctttcgttt
    34981 ttgacaactg tcaagtcaat ctcaggatct tgtttttcta cattgatcgt aggtgggata
    35041 aattgatgtt ctaatgccag caaagttgtc aaggcttcga tcccgccggc tgctcccaat
    35101 aaatgtccag tcatcgattt cgtgctgctg acgtaagtat ctctgaaatt ttcacctaat
    35161 gcgatttgga ttgcttttgc ttctgcctgg tcattggctg gtgtacttgt tccgtgagca
    35221 ttgatatatc cgacttgttc cggctgtaca ccagcttctt ccatcgcaag cttcattgct
    35281 ttagatgcac cagaaccatc tggagtagga gctgtcatat ggtaagcgtc acagtttgca
    35341 ccgtatccaa cgatttctcc atagattttg gcaccgcgag cttgtgcatg ttctaaagat
    35401 tcaagaacta gaacgcctgc tccttcaccc atcacaaaac cattccgatt ttcgtcaaac
    35461 ggaatcgaac cacgattagg atcagttgat tctgagagtg ctgttaaagc tgcaaatcca
    35521 gcaatcccca tctcacaaat cgttgcttct gttcctccag caagaatgac atcagaatag
    35581 ccatgtttga tgttacggaa tgcttcccca attgaattcg ttccgctagc acaagctgtt
    35641 acgatagctg tacaaatacc ttttgcacct acacgcaaag caatatttcc agctgccatg
    35701 ttggcaatcg ccattggtac gaacattgga gaaactcgtt ttgcaccttt ttcatgtaga
    35761 cgaataactt ggttttcgat tgttggaaga ccaccgatcc cagatccgat gatgacgcca
    35821 aaccggtctt gattcatttt ttctttatct aaaccgctca tctccattgc ttctaaagca
    35881 gcgtagattc catagatcga aaacatatcc attcgtttgc tatctttttt aataaaatat
    35941 ttatcgtaag ggaaatcttt gacttcaccc gctaaggtaa tccctgtttc tgaagcatca
    36001 aatttagtga tcggtgcgat gccattcgtc ccctctttta agctattcaa aaagctgtct
    36061 gcgtcatttc caataggtga agtgactcca tagccagtaa ttaccactcg attcatttgt
    36121 ttgctctcct tttcttatcc gtgcatgacc aatccgccat cgacattgat gacttgtccg
    36181 gtgatatatg gattttgtgc taagaaaact gctgtttgtg caatatcttc tacttgtccg
    36241 aaacgctgca tcggaatttg cttttccgct tgttccttga ctttgtctgc taaaacttcc
    36301 gtcatatcag ttgtgatgaa tcctggcgca atggcattac atgtaatgcc tctagttgca
    36361 gcttctcgtg ccacagattt tgttaatcct actacgccag ctttactagc tgcataattt
    36421 gcttgaccaa tatttccaat caacccagat acactagaga gattaatgat ggccccttct
    36481 cgctgcttca tcattttttt caatacgtgc tgcgtcatat tgaaggttcc aatcaaattg
    36541 atatccagac attttttgaa atcttctgca tccattcgca tcacaagttt gtcatttgtg
    36601 attccggcat tgtttactaa cacatggatc gatccaagct tttcttctgc ttctttgatc
    36661 atttgtcccg ctttctcata atcagagatg tcaccggaaa ctcctacgca ttttacaccg
    36721 aatgcttcga tttcttctat cttttctttt ggaatctctc cgcgaccatt caaaatgata
    36781 tttgctccag cttttgcaaa agctaaggcc atcgctttgc cgattcctct tgtactacct
    36841 gtgataaata cattttttcc ttttacatcc ataacgttcc tccttatagt attgctattg
    36901 tttcatcaaa tgtctgctta ttctcaacac gcagaactgg tattgtttta tcgatttttt
    36961 tcataaatcc gcttaatact tttccggggc cgacttcgat gacttgttcg atgccgattg
    37021 tttttaactt atggatactc tcatagaaac gaacaggttg catgacttgc tgttcaagta
    37081 gtcccgcgat tgtttctttc ttcattattt ccgttgttgt gttgcttatg ataggaaaag
    37141 aaggttcaga gaattgaatc tgacttaggt ccttcgccag tttttttgct gccggttcta
    37201 aaatagctgt atgaaaaggc ccgctgacat ttagagggat cattcttttg aacccttttt
    37261 cttttaatag taaaacagct tcgtctactg ctttttcttc gccaccaatc acgatctgct
    37321 gaggtgtatt ataatttgca ggagatacga tgccgtactt gctagcttca tgacagcttt
    37381 cctcgatcgt ttcaatcggc gcattcatca ctgcaaccat tttcccgctt ccagctgggg
    37441 tagcttctgt catataagcg cctctttttg ctactaacgc aactgcttca gtgaaggata
    37501 gcgcattact agctactaat gccgaatatt ctcctaaact cagtcctaaa gctgcatctg
    37561 ggatgattcc atgctccttc aataagcgat aatacgcgat gctgactgtt agaatagctg
    37621 gctgtgtata ttgcgtctga tccaaacgtt cattttctgt aaagcaaagt tcagccatat
    37681 cgtagccgag aatctcactt gcttcatcaa atgtctgctt tacaacggct tcttcatata
    37741 aatcttttcc cataccttga tattgtgcac cttggccact aaataagaat gctgttttca
    37801 tgtttttctc caatatatcc agaatatatg tttgattctt cctattcaaa aaggccgaga
    37861 cattcctatt tcaggaaatg tcccagcccc caacctaatt ttaatgatta tgcagtttgt
    37921 ttttctacgt aagtcactaa atcttgaacc gttttgattc cgtcttctgt ttcgattttc
    37981 acgtcaaatt catcttcaat ttcattgatg atttggaata aatccaagct gtcagcttct
    38041 aagtcttctt ggatgtcagt agtcaatttg atttcttcag tttcttttcc taattcttct
    38101 gcgatgattt cttgaatttt ttcgaatacc ataataatat attcctccga tattgtttgc
    38161 tttttatttt tataatttaa cagtctacaa ctccatgaga atacttcccc atgttaaacc
    38221 tccgccatag cctgtaaata ttactttctg ctgttttcct aaggtgatct ttccactaga
    38281 aactgcttca tccaataaga tagggatgct tgcagcagat gtattcccat atttatccat
    38341 attggtcagg aacttttctt gcggcatctt tacttttcgg gcaattttct caataattct
    38401 gaggtttgcc tgatgcaaaa gcagataatc aactggtttg tctttcatga cttcttggat
    38461 attgtttgca acgtctcgta cggcaaaatc aaaaatatct ctgccagtca tttgcaaata
    38521 atacgaacct tctgaatctt ctgaatagaa cggattttta ttttgctggt agccagcggt
    38581 caatgagcta cttctggtac cgtcagctgc taatttttct ttcagcaatt ttttctgtgg
    38641 attcgcttct aagagaatcc cacctgctcc atcgccaaac aacacagcag tcgtacgatc
    38701 agaccaatcg attagcttag atagtgtttc accaccaatc accagtccct tagttgagcc
    38761 gcagcggatc attttttcag ccatgcttaa cgcataaaca aatcctgagc acgcagcact
    38821 gatatcaaaa gcgatagcat gagttgctcc caatttccct tgtacttgtg ctgctacaga
    38881 tggtgtcgtg taatcgggcg acattgttgc tactaaaata aaatccaaat ctttagggtt
    38941 tagatttttc tgttttatca atttttccgc cactaaaatg cataaatcag aagtctcctg
    39001 atttgttaca catcttcgtt cacgaatacc cgtccttgac gaaatccatt catcagaggt
    39061 atccatcatc ttggaaagat cattattagt aataatcttt tcaggaagga cgctggctgt
    39121 tgcagtaatc attccaaatt gttccattaa atatcctcac ttatagtctt gcaagaagtc
    39181 gtgcaagttt ttcagtgcat ccagcaaggc atgctgttcc tccgtagcca ttccgtctaa
    39241 gatgttcttg accatttcac gatgaaaatg ctgatggacc cgaaatagta actttccttt
    39301 tttcgttagt cccaacttta caacgcggcg gtcatcttca ctgcggatac gttcgacata
    39361 gccttttttt actaacttgt taatagcagt ggtcaaggtc ccaacagtaa ttgatagttc
    39421 tttggctact tctgaagttg tttttttctt gtacatcccg attgcctcga tcgtgtgcat
    39481 ctctgtgatt gacagatctt tgaattgaga ttttttcagc tcagactctt cgattgtcaa
    39541 aatatcattg aagacactaa caagataatc atttactgtt tttaaatctg gttccatgtt
    39601 ccaccgtcca ctaattagtt tgattatcaa atcttttgat gttcaaacta tatcttaact
    39661 tttttcaaaa tgcaacctta aaagatcatt tttttactat atgagctttt tttaagattt
    39721 tactcattta aaacaactga taaatcagca tttataattt cttttattag aaaaatcatc
    39781 tatctcacat attttgatta tcaaagtatt ttctgtgaac agaactatag aattccctaa
    39841 aacttgtcca ttatttcaaa atttgttata gtctttttat tcaaagcgta aaaggagatc
    39901 ctatgttaaa agaaaaaatg aaaactctcc ccatgctgcc gggtgtctat cttatgaaag
    39961 accgagatgg taaggtcatc tatgttggta aagcaaaacg gctcaaaaat agagtaagca
    40021 gctattttca tcagaataaa cagcattcaa aaaaagtatt acgtatgatt catcatataa
    40081 cagattttga tttcgtcgta gtagacacgg aattagatgc tttattgctc gagtgtcaat
    40141 tgatccaaca ttaccgtcct ttttataacc gacaaatgaa ttatttcagt aattataatt
    40201 atgtacatat taccaataag ggatttgtat taacagatac tcctacggct cgaacatacg
    40261 ggccttttcg tctctataaa aaaatgccgt ctattttgcg tataatggaa gaaacttatc
    40321 aaatgccttg gttgtctgaa attagtttgt tagctttgcg agtccagctt cctgacctac
    40381 aggaaatgac ttttgaacag aaaaaaaagg aactccaagg gctgtttcaa ggccgaaata
    40441 aaaagctact gacttacttg aaaaaaagac aacaacattt catctatcag ttgaatttcg
    40501 aaaaagccgg tatgcttcaa aaagatatcg aattagttac ttattttatt agacggatcc
    40561 aggaacaaaa gcagttttta agaacaccat cgctcacctt cagcatgcca ttagcagcag
    40621 atgagagtca gaaaaagcac tacctcgttt gttatggtca acttgcagaa acaatgatta
    40681 cctctggtga cgtttctcct gacttctatt atgaaaagaa agaagctcat ttatctttga
    40741 aacgtcaatt gtcaaaagaa gaaatcgatc ctgtacaaat cttgatcagt taccgcaaaa
    40801 aattagaaaa agaacagttt gaaattgaac tgttaaacaa aaaagaagct gaaaagcaac
    40861 taaattagtt gcttctcggc ttcttttagg catcttcaac ttcaaatgtc aattatccta
    40921 aaatttattg gattaagtca tagatttcca tcgctacgat atcgatatta tcaaattcgt
    40981 attgctgatg agtatctgtt tcagttaatt caaatgtatc ggtcgcttta tcataagtta
    41041 ctgtacactt ctctacgcct tctttttcaa aacggcgaac ttgcagttca ttatctgctg
    41101 actcgaccat agcctccagt cgttttatga ttgcgactaa ttgtgaatgt gtcatcggag
    41161 gtcctccttt tcttatttct gttatattgt atcaaaagca tagcctactt gcattgattt
    41221 tctctaaaaa aactcgtttt ttatgatttt ttttctggta tttttccttc aggccaccaa
    41281 agtttgatca aagcttgggt tccgtcattc tcaaaacctt tatctgccaa ctcttcatat
    41341 agtttcaatg cttgggtagt tgctggcaat acaagatcca ttttttctgc ttcttctaat
    41401 gcaatcttta aatctttgat aaaatgtttg acaaaaaaac caggtgtgta atcttctttt
    41461 aaaatacgag gagaataatt gcttaaagac cagttagctg cacttccacc tgaaagcgtc
    41521 tcaatcactt tctttaaatc aagtccgttt ttttgtgcat agacaagcat ttccgtcatt
    41581 cctgtcatcg tacctgcaat catgatctga ttagccattt ttgtatgctg acctttccca
    41641 gcagaaccat gcaatgtaaa tgtcgtacca aattctttga ataaaggcag tacttgatca
    41701 taaacggctt cttctccacc gaccatgatc gtcagtgtac cattctttgc tcctaagtca
    41761 ccaccggata ccggtgcatc taacgcatct gcgccttttt cttttgctgt ttttgcaatt
    41821 ttttctgcca ggcttggtgt acttgtagtc aaatcaacta aaatatgtcc tgagatatct
    41881 gcagaaaaaa tccccgactg tccatagtag atttcttcta catctttggg gtaacctacc
    41941 attgtaaaga gaatattact ttgttctgct accgcttttg gtgtatctgc ccagatagct
    42001 ccttctgtta ctaaaggatc agcttttgct tttgtccgat tatagacagc tacttcgtgt
    42061 cctgcttcta gtagatggcg tgcgacagca cttcccatga cgcctgttcc gataaaacca
    42121 agtttcattt tttcgacctc ctaaaaatca aaaatccttg cttctatgct agtatacgct
    42181 agaaacaagg attttttcat aaaaaatgtt tgaattttat cttttaaatg aatttaattt
    42241 tattttgatt cgttcaatcg agtcttcttc ttcttttctt cgcatctctt cttcatgata
    42301 aacaaccatg tcttgataaa attcgatcat agcttcgccg aaatgtgtag atgaaatatc
    42361 ataaagttta gcttctaaaa cttttggatc tttaggaact tcactggaaa tataatcaat
    42421 aagtgctggt gcaaagtctt cgtcggtttc aaaagttttc cctaatgaag gatgatcgaa
    42481 caatcggttc aaataagcat ttccttccac gacacaaggt gttccggctg ccatcgcttc
    42541 tgtataagta agcccttgtg tttctgaagt agaagcactg acaaaataat ctgccgcttt
    42601 ataataaagt gctacatcat tgttgtccac ttctcctgtg aactgcacaa attcttctaa
    42661 ctgcagttct gagatcaatt cctctaactc ttctacataa ggtccttttc ctacgataac
    42721 taatctggcg ttaggataag cggagatgac ctgaggaaac ccattaatca aggcttggat
    42781 atttttctca taagaaatcc tgcttaaaga aagaatcatc aaatggtttt cctgaagacc
    42841 tagtgactcc cttaatttgc tgatatcctg ctgagtgata tcagaacgtt tgaatttttc
    42901 aatgtcgata ccagtaggaa tcacgcgaag cggtgctatt acaccatagc tgcgtaatgt
    42961 atcaataaca cgttcacttg gacaaactac acctgtcgta tgatttgcaa ataatctaga
    43021 caagtatttt acatgggatt gcctgatcac ttttccttta ccgatataat gaaggtagtc
    43081 ttcatacatc gtatggtaag tatggatcag aggtattttc aacttcttag ctaccatctt
    43141 cccaagtatc cctgctccga attctgtatg ggtatgaata agatctagtt ctagttcttt
    43201 ggcaaccaaa taagcatacc acatgcctct aacaacaact cttcgatcct tgaaagaaat
    43261 aaatggtaca cttggcattc gaatcacatc tttttccaac ttatccgcat taggatcggt
    43321 cgttgtaaaa atatagactt catgcccatg tctttctaat tcttgtttta atgttttgat
    43381 cgatgtagca actccgctga cttgcggaaa gtaggtatcg gtaaaaaagc cgattttcat
    43441 gtttttcgcc tccaaactag tccttgacct gatttacttt ttttaatttg ctagagactt
    43501 tttcattcag tacactttcg tatacatttt taagttcttc accgatttta ttgatacttc
    43561 gctcttttgc tgtttgataa ccggcttgtg tcgtactcgg tagttcttga ttggtaatct
    43621 gttggatcag tcggataaat tcttcgtttg tcttccccat atagcagttc ttgctatctt
    43681 ctaaccagcc attatacact ggaatatctc gaacaagtac gttttgtttg cttgctaaag
    43741 cttctaaaac aacgatccct tctgtctctt cataagaagg gaaaaagaat agatctgcac
    43801 ctgaatacgc accttctatg atctctccac gaatgtaacc aggaaacagg acattttcag
    43861 gatgatcttc ttttacaatt ttccggatgt tccgaggaat cgagtacatg ggaacatgac
    43921 caaaccaaat aaatgtgtat tcgggcatct tcttagcaat ttctacgaaa tcaatgattc
    43981 ctttacgttc gaaaaacaaa ccgacacaaa tgattacttt ttgatccgga gacagtttga
    44041 aatattctct gaacttttgt tctttcattg gatcagggtg atatttctct aagtcgatcc
    44101 cattggaaat agcttgaatc ggcacacgga taccatagct ttccaacaac tgttttgaat
    44161 aaggtgttgg cgtgatcaga tagtccgctt tttggtacaa tcctaccaga tattttttta
    44221 cgataggaga aagctggttc gatccgataa aggaatttcg gaaatcttcc tctgtagaat
    44281 gcgcatgata gacaactttt tttccttctc ttctggcttt attgaccata ttatgactat
    44341 tgattccgta tgtattgatg tgcaggatgt cataatcttc tttttcatct agtgtataag
    44401 agataccgac ttccgtcaga gcacgttttt gatgatcaag cgcacgcccg attccagatt
    44461 ttgcaagcat cttttcactt tcaaaatata ataatacctt caccttacgt cccccttcac
    44521 ttatatagta taacatagcc tacaaactgt cactcaaact taagccctat ttttgtctat
    44581 tcttactgac aaaaacgttt tcttaaaaaa gaacaaaaaa actggcgtag ctcttttaca
    44641 agccacacca gcttttgata cgttgtttat tttacatatt catgaacaag ttcaacgact
    44701 tcttccatca tatcgcattc tttcaatgca cgatcagcaa gttcagccat tttcgctgtg
    44761 tccaagcgtt tcattaaact acgcgtttta agaatagatg tcgcactcat tgagaactca
    44821 tctagaccca tacctactag caatggaaca gctgtttgat ctccagccat ttctccacac
    44881 atacctgccc attttccttc agcgtgtgct gcatcaatta catttttgat caaacgtaaa
    44941 attgatgggt tatatggttg gtacaagtaa gaaacgcgtt cgttcatacg gtctgctgcc
    45001 attgtgtatt gaatcaaatc gttcgtacct acagagaaga aatctacttc ttttgcaaac
    45061 ttgtctgcta agactgctgc tgctggaatc tcgatcatga tgccgacttg gatcgtatca
    45121 gatacttcct tgccttcact tatcaatttt tgtttttctt cttcaaagat cgcttttgct
    45181 gcgcggaatt ctttgagtgt agctaccata gggaacatga tacgtaaatt accatgaaca
    45241 gatgcacgaa gcaatgcacg catttgtgta cggaacattt cgtcgccttg ttcagataaa
    45301 ctgatacgca atgcacgata tcctaagaat ggattcattt catgaggtaa ttgcagatat
    45361 ggtagttctt tatctccacc gatgtccatt gtacgaacaa ctacaggttt tccttccata
    45421 ccttctaata cagctttgta agcttcatat tggtcatctt ctgttgggaa gtctggagaa
    45481 tccatataca ggaattcagt acggtacaga ccaactgctt ctccaccatt attatggaca
    45541 ccaactagat cttttggtgt tccgatgttt gcagctaatt caaaatgctt gccgtcagct
    45601 gtgacagttt ctgcattttt taatttttcc cattctgctt tttgtgctgc gtaatctgca
    45661 cctgcttttt caaattcagc tttttgttca tctgtaggat cgatgattac atcgccttcg
    45721 atcccgttga cagccaaaat atcgccttct ttgactttag cagtaatttc ttttgtccca
    45781 acgattgctg gaatttctaa agaacgtgcc atgatagcag aatgtgaagt acgtccacca
    45841 atgtctgtta caaacgcttt tacaaaattg cgatctaatt gtgctgtatc acttggagtc
    45901 aagtcatgtg caacaacgac cacttcttcg ttgatcattg atggatttgg taaagtaacg
    45961 cctaataagt gcgctaaaat acgtttagca acgtcacgaa tatctgcagc acgctcttgc
    46021 atataagcat tatcttccat tgcttcgaac ataccgatat acatatccgt tacttctttt
    46081 agcgctgatt cagcatttac gctgttgtct ttaatatttt gcttgatttg acctaccatt
    46141 tctggatctg aaagaaccat caaatgggca tcaaatactt gcgcttctgc ttcacctaag
    46201 ctttgcgctg ctttttcacg aatttgctgc aattcttcag tagatttagc taatgcagca
    46261 tccaaacgag cttcttcagc cgcagtatct tctactgttg ccttactgaa tgttaaatcc
    46321 ggttgaacta gcaggtaagc ttttgcaacg gctacaccgt cactagcggc aatccctttt
    46381 agcatttcaa ccattattca gataagcctt ctttcttcat tgtttcaacg attgctgcca
    46441 ttgcatcagc ttcgtcagca ccttctgcag taatagtaac atcagaacct tgacctacac
    46501 ctaaagacat aacgcccatg attgatttca agtttactga tttaccttta tactctaagt
    46561 ttacatcaga gttaaatttg cttgctgttt gtactaatag tgtagctgga cgagcgtgga
    46621 tcccagtttc tgctactacg tgaaattctt tcttttccat attaatcggt ctcctttaag
    46681 tgagtgttgt ttttatgtga gggttttctt ttttagtaat tcagagaatc ctaacaaatg
    46741 ccaccctttc atctgtaaag attaacattt tttttcgtta gatacaacag ttttcattca
    46801 atttcagata aaagtattca aattattccg gattcttttt catccttttc ttcacatttt
    46861 ataacaaacg tgtttatttc ctttttcttg tcgctttctc ttctcctgac gtttatcatt
    46921 ctttcctttg accctctttg ttttcatatg aaacgaatta cttgcttccc tttttatttt
    46981 tagtataata agagtcaaag gtcaggaatg gtcaatagag atttcacctt ttctacctca
    47041 taaatctctt aaaaaactag gggttattct tttacctcat gaaaaacgag atgtaaaata
    47101 tggatgtacc catttttgtg aaaggacgtg aatgattcat gctttgtcaa aactgcggca
    47161 agaatgaggc aacgattcac ttgtatgcca ctgtcaatgg tcagcggaca caactcgatt
    47221 actgtcagag ctgttatcaa aaaatcaaaa atcaacaaaa tggaggtctg actaatatgg
    47281 cccaaaatga tccatttgga tttggcagtt tggacgatct tttccgttcc atgtctcgcc
    47341 aaatgcaaca gcaaggaaat tatgagcaaa caccaccaac acaatttggc ggaaacaaca
    47401 acttcaatgg tggtcagcca cctcaaggca atacagaagg tttattggga gaatacggaa
    47461 tcaatatcac agaacaagca cgacaaggtg atatcgatcc tgtgatcgga cgcgatgacg
    47521 aaatcaaacg agtcattgaa attttaaatc gccgaaccaa gaacaatcct gtcttgattg
    47581 gagaaccagg tgttggtaaa acagcagttg ttgaaggttt agcacaaaaa atcgtagatg
    47641 gtgatgtacc gcaaaaatta atggacaaag aagtcattcg tttagatgtc gtatctttag
    47701 tccaaggtac tggtatacga ggacaatttg aagaacgtat gcaaaaattg attgaagaga
    47761 tccgtcaagc agaaaatgtg atcctattca tagatgaagt tcacgaaatc gtcggtgctg
    47821 gttctgctgg tgatggaaat atggatgctg gtaatatttt aaaaccagct ttagctcgag
    47881 gagaattgca aatggttggt gctactacct taaatgagta ccgcatcatc gaaaaagacg
    47941 ctgctttaga acgtcgtatg caacctgtac gggtagatga accaactgtc gaagaaacga
    48001 tcagtatttt gaaaggtttg caaaaacgtt atgaagacta tcatcatgta aaatatactg
    48061 atgaggccat cgaagcagcc gcaactttat ccaatcgtta cgtccaagat cgcttcttgc
    48121 cggataaagc catcgatttg ctagatgaaa caggttctaa gaaaaacttg acgatccaaa
    48181 tcgttgatcc taaaacaatc gagaaaaaac tgcaagaagc agaagaacaa aaagttttag
    48241 cttctcgaga agaagatttc gaaaaagcag cttattatcg cgatcagatc aataaacttc
    48301 aaaaaatgaa agaacgtcag ctcactgaag aggaaacacc tgtgattacc gaaaaagata
    48361 tggaaaaaat cgttgaacaa cggacaggta ttccagtggg tgaattaaaa gaaaaagaac
    48421 agacacaact gaaaaactta gcagatgact tgaaagctca tgtcatcgga caagataacg
    48481 ctgtagatcg agtagcgaaa gcgattcgcc gaaaccgtgt tggattaaac aaacagaatc
    48541 gtccaatcgg ctccttctta tttgttggac caactggtgt aggtaaaaca gagcttgcga
    48601 aacaacttgc ctacgaacta ttcggttcac aagattcaat gattcgtttc gatatgtctg
    48661 aatatatgga aaaacatagt gtctctaaat tgattggttc tcctccaggt tatgtcggat
    48721 acgaagaagc tggtcaatta acagaaaaag tacggagaaa tccttacagt cttgtcttgt
    48781 tagatgaagt agaaaaagct catccagatg ttttgcatat gttcttgcaa atcttagatg
    48841 atggtcgatt gacagatgca caaggaagaa cggtcagctt taaagatacg atcatcatta
    48901 tgacaagtaa tgccggaact ggtaaagtag aagcaaacgt tggttttggt gcggcacgcg
    48961 aaggtgttac tcgttctgta ttgaaccaat taaacaacta ctttacacca gaatttctga
    49021 accgttttga cgggatcatc gaattcagtg cattatcaaa agaaaacttg atgacaatcg
    49081 tcacactaat gctagacgat gtcaaccaaa tgctggcagc acagcaactt cacatcgaag
    49141 ttcctacgaa tgtcaaagaa aaattagttg acttaggcta tgatccatct atgggtgctc
    49201 gtcctcttcg acgcacgatc caagaacaaa tcgaagatgg tatcgctgaa ttttatcttg
    49261 atcacccttc tatccatgag ttgaaagcaa aactggataa agatggaaaa atcatcgtta
    49321 cttctaaacc agaacgtttg gctaaagaat cagccgaaga aacggctgaa taaatccagt
    49381 tttatagcgt gagacggtat ttctttgaga aatgccgtct cattcttttt tcagaaaagt
    49441 caaaatactg ttcattttat aaaaaagaaa ttataataag agaaacaagc aattaaaaat
    49501 acataaaaga ggtgagcgcc atgaaattgg taaatgtaac aaacagccat tcccgtcttg
    49561 tattaaacca gcttgaaaac actgatgccc atctagtcaa ggtctacaca gccggaaata
    49621 ccactattgt ctacactgaa gcccctgaac ataatgaaat tctgctgatc aatgacaaac
    49681 gtaaaatcca gccgaaagaa atcgaagatg ctaaaaatta tttcttagat aaaatcaaag
    49741 acgccgttta ccacgaggat gaaatcaaag taatcgaatt ggcaggcctt gttgaaatct
    49801 cgattccaaa acatgaatta ggcgctacat tagccgtgta aacaaatttt gaacataata
    49861 attaaagagc gtgggacaaa a
//
LOCUS       DACEQH010000017        48157 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.017, whole genome shotgun sequence.
ACCESSION   DACEQH010000017 DACEQH010000000
VERSION     DACEQH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.017"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(862..1203)
                     /locus_tag="IW833_000997"
     CDS             complement(862..1203)
                     /locus_tag="IW833_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1307131.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1203..1433)
                     /locus_tag="IW833_000998"
     CDS             complement(1203..1433)
                     /locus_tag="IW833_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1307132.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIGDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1720..2058
                     /locus_tag="IW833_000999"
     CDS             1720..2058
                     /locus_tag="IW833_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307133.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2263..2502)
                     /locus_tag="IW833_001000"
     CDS             complement(2263..2502)
                     /locus_tag="IW833_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ1307134.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2478..2825)
                     /locus_tag="IW833_001001"
     CDS             complement(2478..2825)
                     /locus_tag="IW833_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ1307135.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(3557..4138)
                     /locus_tag="IW833_001002"
     CDS             complement(3557..4138)
                     /locus_tag="IW833_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1307136.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4321..4947)
                     /locus_tag="IW833_001003"
     CDS             complement(4321..4947)
                     /locus_tag="IW833_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1307137.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4969..6300)
                     /locus_tag="IW833_001004"
     CDS             complement(4969..6300)
                     /locus_tag="IW833_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ1307138.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6455..6856)
                     /locus_tag="IW833_001005"
     CDS             complement(6455..6856)
                     /locus_tag="IW833_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1307139.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6942..7148)
                     /locus_tag="IW833_001006"
     CDS             complement(6942..7148)
                     /locus_tag="IW833_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307140.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7243..8745)
                     /locus_tag="IW833_001007"
     CDS             complement(7243..8745)
                     /locus_tag="IW833_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ1307141.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8763..9218)
                     /gene="argR"
                     /locus_tag="IW833_001008"
     CDS             complement(8763..9218)
                     /gene="argR"
                     /locus_tag="IW833_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1307142.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9316..9933)
                     /locus_tag="IW833_001009"
     CDS             complement(9316..9933)
                     /locus_tag="IW833_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307143.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10154..11527
                     /locus_tag="IW833_001010"
     CDS             10154..11527
                     /locus_tag="IW833_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1307144.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11602..12714
                     /locus_tag="IW833_001011"
     CDS             11602..12714
                     /locus_tag="IW833_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ1307145.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTVHWPTEDYQKDISTYITKNPS"
     gene            complement(12786..14423)
                     /locus_tag="IW833_001012"
     CDS             complement(12786..14423)
                     /locus_tag="IW833_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ1307146.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14591..15199)
                     /locus_tag="IW833_001013"
     CDS             complement(14591..15199)
                     /locus_tag="IW833_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1307147.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(15583..16734)
                     /gene="queG"
                     /locus_tag="IW833_001014"
     CDS             complement(15583..16734)
                     /gene="queG"
                     /locus_tag="IW833_001014"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ1307148.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(16753..17505)
                     /locus_tag="IW833_001015"
     CDS             complement(16753..17505)
                     /locus_tag="IW833_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307149.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(17646..18596)
                     /locus_tag="IW833_001016"
     CDS             complement(17646..18596)
                     /locus_tag="IW833_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1307150.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(18597..19436)
                     /locus_tag="IW833_001017"
     CDS             complement(18597..19436)
                     /locus_tag="IW833_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1307151.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(19433..20179)
                     /locus_tag="IW833_001018"
     CDS             complement(19433..20179)
                     /locus_tag="IW833_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1307152.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            20417..21787
                     /locus_tag="IW833_001019"
     CDS             20417..21787
                     /locus_tag="IW833_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1307153.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(21842..22738)
                     /locus_tag="IW833_001020"
     CDS             complement(21842..22738)
                     /locus_tag="IW833_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1307154.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(23019..23696)
                     /locus_tag="IW833_001021"
     CDS             complement(23019..23696)
                     /locus_tag="IW833_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1307155.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(23787..24692)
                     /locus_tag="IW833_001022"
     CDS             complement(23787..24692)
                     /locus_tag="IW833_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1307156.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTAVSWTT"
     gene            24867..26321
                     /locus_tag="IW833_001023"
     CDS             24867..26321
                     /locus_tag="IW833_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1307157.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            26337..27368
                     /locus_tag="IW833_001024"
     CDS             26337..27368
                     /locus_tag="IW833_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1307158.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            27375..28562
                     /locus_tag="IW833_001025"
     CDS             27375..28562
                     /locus_tag="IW833_001025"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ1307159.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            28718..30100
                     /locus_tag="IW833_001026"
     CDS             28718..30100
                     /locus_tag="IW833_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1307160.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            30072..30458
                     /locus_tag="IW833_001027"
     CDS             30072..30458
                     /locus_tag="IW833_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307161.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(30658..31326)
                     /locus_tag="IW833_001028"
     CDS             complement(30658..31326)
                     /locus_tag="IW833_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ1307162.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(31515..32312)
                     /locus_tag="IW833_001029"
                     /pseudo
     CDS             complement(31515..32312)
                     /locus_tag="IW833_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(32593..33972)
                     /locus_tag="IW833_001030"
     CDS             complement(32593..33972)
                     /locus_tag="IW833_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1307163.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            complement(34147..35316)
                     /locus_tag="IW833_001031"
     CDS             complement(34147..35316)
                     /locus_tag="IW833_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1307164.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            complement(35291..36442)
                     /locus_tag="IW833_001032"
     CDS             complement(35291..36442)
                     /locus_tag="IW833_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ1307165.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            complement(36402..37763)
                     /locus_tag="IW833_001033"
     CDS             complement(36402..37763)
                     /locus_tag="IW833_001033"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ1307166.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            complement(37760..38761)
                     /locus_tag="IW833_001034"
     CDS             complement(37760..38761)
                     /locus_tag="IW833_001034"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ1307167.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            complement(38777..39472)
                     /gene="dapD"
                     /locus_tag="IW833_001035"
     CDS             complement(38777..39472)
                     /gene="dapD"
                     /locus_tag="IW833_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ1307168.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            complement(39478..40194)
                     /locus_tag="IW833_001036"
     CDS             complement(39478..40194)
                     /locus_tag="IW833_001036"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ1307169.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            complement(40191..41072)
                     /locus_tag="IW833_001037"
     CDS             complement(40191..41072)
                     /locus_tag="IW833_001037"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ1307170.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            complement(41075..41875)
                     /gene="dapF"
                     /locus_tag="IW833_001038"
     CDS             complement(41075..41875)
                     /gene="dapF"
                     /locus_tag="IW833_001038"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ1307171.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            complement(41862..43121)
                     /gene="lysA"
                     /locus_tag="IW833_001039"
     CDS             complement(41862..43121)
                     /gene="lysA"
                     /locus_tag="IW833_001039"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ1307172.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            complement(43607..44170)
                     /gene="efp"
                     /locus_tag="IW833_001040"
     CDS             complement(43607..44170)
                     /gene="efp"
                     /locus_tag="IW833_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ1307173.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            44598..45515
                     /locus_tag="IW833_001041"
     CDS             44598..45515
                     /locus_tag="IW833_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ1307174.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPNGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            45528..46673
                     /locus_tag="IW833_001042"
     CDS             45528..46673
                     /locus_tag="IW833_001042"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ1307175.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            46844..47302
                     /locus_tag="IW833_001043"
     CDS             46844..47302
                     /locus_tag="IW833_001043"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ1307176.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            47461..47931
                     /locus_tag="IW833_001044"
     CDS             47461..47931
                     /locus_tag="IW833_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ1307177.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(47984..48099)
                     /gene="rrf"
                     /locus_tag="IW833_001045"
     rRNA            complement(47984..48099)
                     /gene="rrf"
                     /locus_tag="IW833_001045"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        13617 a         9923 c         8624 g        15993 t
ORIGIN      
        1 atttttactc attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat
       61 aataatattg acaaaaagtg actcaaaata aaaaaaaact tacattatac tggaatgaac
      121 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
      181 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
      241 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
      301 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
      361 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
      421 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
      481 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
      541 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
      601 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
      661 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
      721 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
      781 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
      841 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
      901 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
      961 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
     1021 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
     1081 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
     1141 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
     1201 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
     1261 tcggcctctt ccccaccact aaatggatct ccaatttttg gaactaacgt aattgttcca
     1321 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
     1381 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
     1441 actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac
     1501 tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt
     1561 agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc
     1621 caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg
     1681 ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa
     1741 gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt
     1801 gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa
     1861 cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta
     1921 gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc
     1981 tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa
     2041 gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac
     2101 gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag
     2161 tatatgcagc ttatgagtat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc
     2221 tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac
     2281 taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg
     2341 ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg
     2401 gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata
     2461 aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc
     2521 attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
     2581 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
     2641 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
     2701 agatttgacg gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc
     2761 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
     2821 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
     2881 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
     2941 gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
     3001 aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct
     3061 gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
     3121 gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct
     3181 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
     3241 tatgagcagc tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca
     3301 acctctccac taaacgatgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa
     3361 aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca
     3421 caacctctaa tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac
     3481 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg
     3541 tcacaacctc taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt
     3601 gaaaattctg cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct
     3661 ggatttcagc aatcaacgct ttggcttgac ggtctgcttc ttcatcggat agtttttctt
     3721 cagaaagtgc caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta
     3781 gcaacagacg gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac
     3841 agccatgata agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta
     3901 atgcttcttc tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata
     3961 cgtccatatg cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat
     4021 aaaattgacc ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat
     4081 agcctttttt ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg
     4141 atattctcct ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct
     4201 tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg
     4261 ttctcatata gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc
     4321 ttatgatagt tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa
     4381 gttcgtcaac gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac
     4441 cacaacattg aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc
     4501 gttcattcca ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc
     4561 attgctttct ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata
     4621 aggtttcaat tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact
     4681 ttttctagca aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca
     4741 tttatggtca tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt
     4801 tgcttgtttt accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt
     4861 tgtcaaacca aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac
     4921 tactgctgta tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac
     4981 atcatttaga gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata
     5041 aggaagtttt ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc
     5101 atagatactt gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc
     5161 tttaggatct tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt
     5221 gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg
     5281 agtgataaat acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc
     5341 cttcaactga tccataatga ttcgataatc atctagagag atataagtga actgcaatcc
     5401 gcctttgacg tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac
     5461 agcgattgct ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt
     5521 acttggtttt cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc
     5581 atcttctgta aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac
     5641 acctagatgg aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc
     5701 ttcatcttca cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta
     5761 ttcattgaac attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt
     5821 ttccggtaat tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt
     5881 caatcctttg attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc
     5941 ttcaacaatc actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg
     6001 tgctgtaccg tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc
     6061 gcgaagcatg cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct
     6121 ttctccattg aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc
     6181 atacatacgt gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt
     6241 cgctaaagtc ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat
     6301 ttttccctcg cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt
     6361 ctattctcca aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc
     6421 acgtttttag tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga
     6481 tatttcctct agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt
     6541 gggcttcttc ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc
     6601 cgccatcatc taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc
     6661 ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa
     6721 ataattgttc tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca
     6781 tcgagccatt tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt
     6841 cgtatgcttt tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt
     6901 atagtgtatg acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg
     6961 ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata
     7021 tttgttccaa aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga
     7081 tacatatata ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc
     7141 gttaccattt tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac
     7201 tttttttgcc gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga
     7261 ttcaagatca gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt
     7321 tccgaattca tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat
     7381 caaaaaccag ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc
     7441 cgggtcgaag ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt
     7501 gtttccttgt tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac
     7561 gggctgtttc aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg
     7621 caaaagcatc aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt
     7681 gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag
     7741 ttctaatgct ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc
     7801 tttttcttca atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt
     7861 tcctggatgc tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat
     7921 cactgttcca gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc
     7981 aagatctgct tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc
     8041 aggatttggg atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga
     8101 atccgcacct aagctattca atactttttc agcaaagaat ccaccagcac catttccggc
     8161 atcaacaatg attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt
     8221 aatttttgtg actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc
     8281 agtttgttcg ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc
     8341 tgctcctcct tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc
     8401 agtcagcata accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc
     8461 aggcgtagta gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat
     8521 caacgcctct ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca
     8581 ttttttattt ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc
     8641 gatctttttt accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat
     8701 tcctcgaata tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc
     8761 atttattccc tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg
     8821 caaataataa tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact
     8881 tcatctagcc aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg
     8941 aactgaacag actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca
     9001 tttaaagaaa cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga
     9061 ctttttacta acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg
     9121 cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg
     9181 atctggcgga tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga
     9241 ttatagcaaa agataagtaa tatttcttca tattttggta ttttttactt ttattttttg
     9301 ttttcattca ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc
     9361 aggtgctgat tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc
     9421 atagccttct tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata
     9481 gggcccgacg tgtaagattt gggcacaaag accttcttcc atctttttga atactacttg
     9541 ttctttcaag tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc
     9601 tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac
     9661 tggttcatgc aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata
     9721 catctgataa tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac
     9781 tgcatataga caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct
     9841 gatgctaaag aatgattttt taggaacctc tagtatttgc gggctttttt tcatactata
     9901 gctcttgtct ttacgccatt caattttttc catttttctt cctccgcgaa attccattta
     9961 gttatttatt atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc
    10021 agtttgtgct agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg
    10081 agagagaggc ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt
    10141 agaggtgatg taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac
    10201 agggatgatg tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac
    10261 tcttatcaga cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct
    10321 tttgatgatt tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa
    10381 aaaactcttt ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc
    10441 tcctaccttc atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc
    10501 tcttccgttg atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat
    10561 gatgggtgtc ggtactttga caacggccat tgctccagca attggcccca cttatggagg
    10621 cattatgact tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgat
    10681 tctttctttg attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta
    10741 tttggattgg ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt
    10801 gagtacgttg ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt
    10861 ccttttttac tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc
    10921 aaataaaaca ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg
    10981 tgtatctttt gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc
    11041 tggattagcg atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa
    11101 aatgctggat caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat
    11161 tggttggcta gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg
    11221 tcatgtgttt tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat
    11281 gaatgcactg aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca
    11341 gttttctggc gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca
    11401 tacttcccat aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt
    11461 gttgctgttg cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa
    11521 agcgtagatt ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg
    11581 tttgtaaaag gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt
    11641 atgaccctag ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg
    11701 acaggacagg caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga
    11761 aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa
    11821 gatttccaac ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa
    11881 aaagatttag ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt
    11941 ggaaacatcg aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca
    12001 gcagtttcac tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat
    12061 cttggtcagc gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat
    12121 aagaagagcg attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta
    12181 attttgacac ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat
    12241 atacgtccgc aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac
    12301 tggcctgttg ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc
    12361 cttttaggtg ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct
    12421 tataagggat tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac
    12481 tggccatacg agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc
    12541 ggcgtactgc cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg
    12601 accacaggtc cttatatcgg ctatctatta gccaacttct taaatactgt tcattggcct
    12661 acggaagatt atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac
    12721 agctttttca agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg
    12781 ttattttagt tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag
    12841 agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc
    12901 agatttttat aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg
    12961 atgtcttcaa atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg
    13021 attgacaatg tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca
    13081 tagcaggctg tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga
    13141 ggatagacac ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt
    13201 gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct
    13261 aaaactgggt cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa
    13321 acagcatcga tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg
    13381 actgtccgcc catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt
    13441 gtacatgtca cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac
    13501 ggatagtctt gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt
    13561 cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt
    13621 gctaaggtaa tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc
    13681 tggtcattac tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt
    13741 cccaatgccc aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct
    13801 agcacaaccg ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca
    13861 tgtgtccatt cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt
    13921 tgataagtct ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa
    13981 ttgttctcaa atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg
    14041 ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt
    14101 aaaattttct cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt
    14161 gctgttcttc ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa
    14221 tctagttctt cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt
    14281 gctagttgat aagctgtctt cctatcaaaa acttcatgaa cattattacc gtaaaatgtc
    14341 gtttctacaa tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat
    14401 tcgagactct cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct
    14461 ttattataca ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta
    14521 tttttcacac aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc
    14581 ttttatcggc ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac
    14641 cataacgaga tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc
    14701 cgtttaagta tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt
    14761 atgaaccgcc tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg
    14821 ttgcagcagc tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat
    14881 gttcttgcgt tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg
    14941 ttgctgaaga atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga
    15001 tgtttgcgat cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt
    15061 tttgtgagat cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag
    15121 catttgtagt tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa
    15181 ttttcttaaa tgaattcata ttaaactcct ttttttctcc gttttttatt ctttcgacgc
    15241 tattaacttt atcatttaga aatatttcat aggtaaaaga aacatgacat ttaatgacaa
    15301 aataacgaat tatttaatat ttgtaacatt taaacaaata tttttttgat acaaaaccat
    15361 aaaagccttg ttgtataagc aaagtaatcg tttttctatg tttttttatt tttgaatgaa
    15421 aaacgcagat tctgtaacat tcactgcaat aatccataaa aaaaagcaaa aaaatttttg
    15481 atttttatca aattctctta aaaaaatgta aaaaatacaa aaaaaccagt aattgttaca
    15541 acaattactg gacaaaaact ttgaataatg ttaactattt ttttatttta actttctccg
    15601 caataatttc tgggcatgtt ccaattcgtt tataacgact tcttctgttt ctttagctaa
    15661 tatttcatct aatacatcaa gccatttttc cggttctttt gttcctagac gtccaatagc
    15721 ccaagctgct gttcctctga tgacagggcg gacatctgtt aaacattctg aaatctcagg
    15781 caatgccgtt cgatcaccta gattagctaa agcaatcaat gcgtttctct gcaaaggttt
    15841 cttcccacgc catgaaccag ctaaatgccc aaattgctgt ttgaagtctt tattagacat
    15901 cgaaagcatt ggctttaatt ttggatatat ttcgtctact tttggttcca tctcttcatg
    15961 aaaatgaaaa tcttttcctt gattgtaagg gcagactaac tggcagatat cacatccata
    16021 aatgacattt cgcatttttt tacgatattc ttccggcatc atcccttttg tctgagtttg
    16081 ataagacaaa catcgctggg cattcattct tccgtcacca agcaatgctt ttgtcggaca
    16141 agcagtcacg cagcgcatac agtcaccgca accaaattct cctggtttat caggctcaaa
    16201 tacaatatta gtaacgatct caccaagata gacaaatgaa ccgaactctt cagtgatcaa
    16261 caatccgttt cgtccaataa atcctaaacc agctcgctgt gctacagcga catcgatcag
    16321 ttctccagta tctacttgcg gagctagacg ccattcctct tctttttgcc atttttctgc
    16381 tcttgtttgg atgaattcga tcaatcggtt caaacggtct ctgagtatat catggtaatc
    16441 gatcccccaa gaagcacgag caaactgccc gcgtttttca tccctaggca cttcttcatg
    16501 gatcctagta ggatacgcaa gtgcaatgga aatgatcgat ttcggctgat caaatgtcat
    16561 ttctggataa agacgctcat cgatatttgg atgttcaaag ccagaagtat ggccagctgc
    16621 tttttgttct tccaacgatt cttttaatga atcaaaaggt tccgccgtgg tgaacccgat
    16681 tttgtcaatt cctaatcgtt tgctttcttg tatgatttct tcttttaatg tcatggtctt
    16741 cctcgcttgc tgttaacggc tgtataaccc ataatatagt ttttcttttt tcagctggac
    16801 aaactggatc tgctgatcgg caatcttctt ttcttccatc catttttttc ttgcagcaaa
    16861 tacttcccaa taatttgttg aaattaattt tcttttgtta tctaatgcat taaaaaaatt
    16921 caatgaaact ggttttgtac ttaacagttc atggagcctt acctcttccc atggcgaaaa
    16981 ctcagacaga tcgcgggaat acaatacttc ttcatagaga tttttttgct gtgtgtacgt
    17041 gaatgtttgc gggattttcc caggcctagc aatttctact agacgattat aaaacatatt
    17101 cattaatttg ctgttggcaa taaattgact tttataatag gccgactgga tctttttgat
    17161 tactccttct gctttgatga ctgaaaataa tgcaggccat gctttgacgg ttatttcgtc
    17221 gttttgttta actaatacgc caggtgtgaa gccgactgga gaaaggaaca caaaataacc
    17281 gataccggta ttttcatcta aaggctgaaa ttcatcaaac aaccatttgc ctatgtacag
    17341 ctcttcatct ggttctgttg tctcattttg atttgctatg aataatttag ctaattctgc
    17401 ttcaagaaat tgaaggtctt gatccttgtc ggtattttcc tgctggctga tcaatcgaaa
    17461 gacttcttct ttaatcgtat atttatcttt acgtgtaaaa ttcatagcca caccttcctt
    17521 ccttactcta actataacgg aatttcttca aaaaaggtat ctgtacgacg aaaaacagca
    17581 taattttttt tcatatctct tataaaaaat gcaaggtctg ctcctcaagc tagaccttgc
    17641 acttttcagc tttcttttgt ttgtgacaaa ccttcatgga tcgtttcaat attccatttc
    17701 atcatctgat agtaagaatc tccttgttct ccttcttttg ctatggaatc agtgaacagt
    17761 ttatcataga ttttcaaacc cgtttctttt gctacccgtt ccatgcttct tgagtctaca
    17821 ctggtttcca cgaataaaac cggtacttct tttgctctta tctgatcaat gatcgctttc
    17881 atttgatcag gcgtaccttg actttctgta ttgatctccc atatataagc tgctggcaaa
    17941 tcatatgctc gtgaaaaata cttgaatgcc ccttcacttg ttactaataa ttttttgttc
    18001 tcttcgatag aagcaaaact ttccttcgct tgtgtatcta attcttttag tttttccaca
    18061 tatgcttctg catttttttt atagagtgtc gcattttctg catctttttc agagaatata
    18121 cgtgcgattt cctccacata ttttattccg ttagataggt ctagccaagc atggggatct
    18181 gcttttgtat gttcttcacc gctagttaaa taaagaggtt ctacattttt gctaactgca
    18241 aaataatctt ttccttcttc ttttttagcc gtttccatca agttatcgaa ccagctgtta
    18301 cctgtttcaa gattcaaacc gttgtataaa ataacatctg catcgcttgc ctttttgatg
    18361 tcttctggta gtacttcata ttcatgcgga tctgttccga ctggtacgat actgtggata
    18421 tctatttgat ctgttccgac ctcttttgcc atgtccgcca agatcgaatt ggtagccacg
    18481 actgacaact tttctttact tccttcatcc gcttggtcag aagcctgctt tccgcaaccg
    18541 gctaatacca agaaactaac tgccaagatt aaaaagagtg atttttttgt tttcatctat
    18601 ctgcttcctt tctgaaataa aattcctttt gttggagaaa ataaaaaagc aaggataaaa
    18661 aatgcggtac atgctaacac gatcgtagct cctgatggcc aattgtaact ataactgaaa
    18721 aacacaccga caactgcact tagcataccg attcctgcag ctgtaatgat cattttcagt
    18781 aaatggttcg ttagcaagta agccgtggcg gctggtgtga tcaacatggc aatcaccaag
    18841 attgttccta ctgtctgtaa actgactaca gccactaatg tcaaaaagaa catcaaaaga
    18901 taatggatca gcgatgtgtt cagcccataa gcttttgcca ttgtcggatc gaatgatgtg
    18961 atcttcaatt ctttataaaa gaaaaagaca aatatcaaga cgattccact aacgagtgct
    19021 gtcagcaaca aatcgctttc cctaacagct aagacattcc caaataaaat atgatacaaa
    19081 tctgtcgcac tctgggcata tgaaatcaag ataatcccta atgcaaaaaa ggagctaaag
    19141 acaattccaa tcgctgtatc atttttcaat cggctccgct gagtcacgaa accaatcact
    19201 gctgctgtta gcatcccaaa agcagtggct ccaaagatgt aactaaaacc aaacatataa
    19261 gaaatggcta cacctggaag aactgcgtga gagatagcgt ctcccatcaa agacatccct
    19321 cgtaaaataa taaaagaacc gatgactcca gaagcaagtc ctacaagcat ggaagtgacc
    19381 aaagcgtatt gcaggaattg ataattcatt aatccatcaa taaaggattg gatcattttt
    19441 tctctcctcc ttttactaaa tgtctgatga tctcaccata ggccaattga aggttcttcg
    19501 atgtaaagac ttcttgtact ggaccggaag cgatcagctg tttatttaaa agaataacct
    19561 catcgaaata ttcttctact ttatgaagat catgatggac gatcaaaatc gtttttcctg
    19621 aattcttcaa ttcctgcaag atgtcaaaga tctttcgttc acttaacgca tcaatcccta
    19681 cgaatggttc atctaaaaag atccattctg ctccttgggc tagagctctt gcaataaaaa
    19741 ctctctgaag ctgtccaccc gatagttcgc tgatctgtct tttggcatat tcttccatcc
    19801 ctactttttt caaagcttgt cttgcacgtt ctttttcttt tttcccaggt ctttgtccaa
    19861 ttcgtaaaga tggatatgtt cctaaaagta caacgcctaa tacatcaatt ggaaaggaaa
    19921 gatccaattc acttcgctgt tccacgtagg ctattttttt acggactgag tttaccgact
    19981 gtccagaaaa ggtgacttta ccagtagtag ctggtatcaa ctctagcaaa gatttcataa
    20041 atgttgattt tcctgcaccg tttggtccga taataccggt aattttttgt ggatttatcg
    20101 tcaagttgat agttttcaaa acagttttcc cttgataagc ggctgaaagg tcttgaatta
    20161 caatggccgt acgttccaaa tcttacactc ctttttaggt ttacctaact ttttatttaa
    20221 atatagttta actaaaatgg aaagcatttt caagtagaat gatgaaaaag tttttttcga
    20281 attttcctcg cattttatga aaattttctg tagaatcaca tcaagagttc aataaggcta
    20341 acctaataat gaaaaacatg tgttgaaacg tttgattatt ttttcttgtt gactcaacaa
    20401 cgaaaggagt ttttgtatgg aagaagaaaa gttaactcga agtcttagtg cccgtcatat
    20461 ccaaatgatc gcactaggtg gaacgatcgg tgtcggtctg tttatgggtg cctcctcaac
    20521 catacgttgg acagggccct cagtgatggg agcctatgcg attgcaggat tctttttgta
    20581 tttgattatg cgtgctttag gcgaaatgct ttacgttgat ccttcgactg gttcttttgc
    20641 aaattacgcg agcgagtaca tccatcctgt ggctggctac ttgaccgctt ggagtaatat
    20701 ttttcaatat attgtcgtag gaataagtga agtgattgct gtaggatctt atatgaatta
    20761 ttggtggcct gatttgccgg caattattcc cggaatcatt gtcgtcttgt ttcttatgct
    20821 ggcaaatctt atttctgtca aggcgtttgg cgaattagaa ttttggtttt cgatcatcaa
    20881 agtcataacg atcattttga tgatcattgc aggtcttggg gtaatcttat ttggatttgg
    20941 taatcatggt caggcagtag ggatttcaaa tctttggaaa aatggtggat tcttcactgg
    21001 aggcgtcaaa ggatttttct ttgcattatc gatcgttgtt gcttcctatc aaggaatcga
    21061 actgatcgga atgactgctg gtgaagcaga gaatcctaaa aagacgatca ttgaagctgt
    21121 ccagtccact atcggtcgaa tcttgatttt ttatattggt gctattttta tcattgtgag
    21181 tatctatccg tggaatcaat tgagtgagat tggttcacct tttgtacaaa ctttttctaa
    21241 agtcggcatt acatttgctg caggactgat taattttgtc gtgattacag cggcattatc
    21301 aggttgcaat tcagggattt tcagcgcaag tcgtatggtc tatacacttg ccataaatgg
    21361 aaagatgtct aaaaaatttc tgaaactatc tcgtcacggt gcgccttttt atcctgtcgt
    21421 agcaatctca ggtggaatct taattggtct gatcttaaat tatcttctac caatgttcat
    21481 caaccagtca gaaaatattt tcgttttcgt atacagctca agtattttgc ctggtatggt
    21541 tccttgggta gtgattttga tcagcgagat tcgcttcaaa aagaagcacg cagataaaat
    21601 gcatgatcat ccttttaaaa tgccactatc tccttatacg aattatgtga cacttgcttt
    21661 tttagctatc gtattgattt tcatgtttat caatcctgaa acgagaatct cattgattat
    21721 tggactcgtt ttcttgcttt acatgacgat ccattacttt gtacgcgaaa aaagaaccgg
    21781 aaaataaaaa aagaacgaag cttcacctta gtggaagctt cgttcttttt tattgctttt
    21841 atcaattcaa ttttagcgtc tcaccgggag tcaggaaata attctccatg ctcatcccat
    21901 tcagcgacaa gatcgtttct actggtacgc cagttcgttc ggctatttga tttggacctt
    21961 ctccttccaa aactacggtt gtcgcagcct gtgaagtcgc gctgttggta ttagtttcag
    22021 aggaatctgt tgtgtcttgt gttgttcgag cttgcatttc agaagcttct accgactctg
    22081 ctgtctcaga atcagaagat gttgtgtctt gtgtgcttgt tgactctgct tgaagagagg
    22141 cgctagtctg caaataaata tcggaaatct tagcgacatg attcgtataa ttagccgata
    22201 attgattgat caaagtatct aagtctacac cgtctttatt ttctgctgcc tgatacgctg
    22261 cacgtaattt tctgatccct tcgtcggaga ttgcttgaag ctcacgtgct ttttggttag
    22321 caactgctga taaaccagct attccctctt gatttccctg aatttctgtc tggtattctt
    22381 caatcaatgc aggcgtcttt tcttcgatct ttttcgaata atcatttacg attgcttgaa
    22441 gttgctctgg caaaccattt gctgtttttt cttcagaagt ttgtgtctct tttgttttag
    22501 ttgatgactc acttgtttgt ttcgtctctg ttttctttgt cgttgtttcc gttatggtcg
    22561 tacttttcgc ttctgacgaa ttttttccat gaaaatatag acccgcaact cctcctgcta
    22621 tgatcaaaaa cacggcaact gctgccccaa tcatgatcat ccgcttacga gctgcctgtt
    22681 tttgctgtct tcttcgggtc atccgaacat tttctcccca ctcattttca tcatacattt
    22741 ttttcaccac caatttttat gtataatagc gttatcaaac gcttcatatt cttattgtag
    22801 caaataattg tcgctgtgca catttttatc tttgttattt ataagaaaat ttcaccagtt
    22861 gagatgattc caagattgtc cgtcgatttc tccttgcagc tgcattgttc gttcgtctat
    22921 ctcagccatc agtttcttta tttcatataa gagcgcacgg tcttgatatt ctgatttttc
    22981 gcttaattca tctaattgtt ggatcagcca atcatttgtc ataatgaagt tcctttcatt
    23041 tcttgcagtg taaaggtcaa gtcttcgatc tgctcttttg cctcagtcag tgcttctacg
    23101 atatatgctt gtttttcaga ttcttcttcc tgcattttct gattttcctg tactctttga
    23161 ataaaccagt caatccgctg agtcagcgat ggtttgcctt ccaatgagag atactgctga
    23221 taagcaatta gcttctccca acgtttttcc tgggataaat acataaattg tgctaaaacg
    23281 aatggcggaa gataggtcat atggaattta tttgcgattc gctgatacgg cctcaaaaat
    23341 tcggaaatcg tatattcttc ttttcctcca gcttggtatt ctttttccgc cacacctgtt
    23401 gtcaatacga ttcctagttc tttccctttt aatacatgtg ctgctcgttc caagacatta
    23461 tcttgccaaa tttttaaatg agcaggagaa ctataccagt aaaacggaaa ctggaaaatg
    23521 atccgatcat gctgggccaa gagctgtagt tcagtctcta tatctatttt tccgtctgga
    23581 taacttgatt ccaaatgatg ccaagtagct ggtaaaccag cagaagcttc tttgaaaaat
    23641 tgctgattta aagattcttc gatcgttgga tggctaatga tgataagtgt acgcatccct
    23701 tttcctcctt tttagtcttc gctttacagt gtagcataac cgccacaagt ttcttttgcc
    23761 gatgcttgtc attttaacca gactaatcag gttgtccaag aaactgcggt ttgtttttcc
    23821 aactgttttt tcaaggtatc caaagcaata ttcttaaaat ttttgttttt catacgttta
    23881 cgatagttcg tagccgtttc tccggtactc tttttgaaca ttcggctaaa tacgactaga
    23941 ctattgaacc ctacttgata ggcaatactt tccacggatt catccgtcag agaaagaaga
    24001 aacgcccctc gattgatccg taccccgatc aaatattcct tgatactggt acccatatga
    24061 tttttgaata ctcgcgacaa atggctccga ttgaccgcta aatgctgggc gatttcctca
    24121 acagacaatt tttcagcata atgtgcctcg atgtatttga ctgtttcaac agctaaagag
    24181 gaatgaacct tttgtacacc accgctgacc tttccgccat cttttaaaag gactgataaa
    24241 aaacggtagg ttaatgctgt taactgtaat tcattttcca aggtttcttc cgtatctttc
    24301 atgcattcta aaataatgtc gatatatttt tgtatgtcag aagagaccat cgtgtattgg
    24361 tcttctctga aaagcgactg ccgaatgatt tcttctgcag cactcccacc aaatgaaagc
    24421 cagcaataca tccacggatc ttctccatct gataagtaaa acgtcgagtc tcctggacga
    24481 atcaagaaca gatcaccttt tttcaattgg aattgttgat ttttcacatg ataagatcct
    24541 ttgccttcca tcacgacatg caggatgtaa tcattgcgaa tagcaggacc aaaagaatga
    24601 aacggcatcg ttttagagaa accacaaaaa tcaaaataca attctttttc tacttgttga
    24661 tgttcctttt ttaaaaaaat cgattgctcc acgtctatcc ctccttactc ctttagtata
    24721 atgcaacaaa aagttaaata aaagtcacag aaatatatta aaaaggaagc gatttcttgt
    24781 taagtttaag tcattaaaag atctttgtta ttcgttgtaa ccggtttact tagtattata
    24841 tattgaacag gagaggaata cagattatga aaagcaaaat gatccaacgt gtgtcctacg
    24901 ctttaggcgc ttttggacac gacacattct atgttacatt aagcacttat tttatggtat
    24961 tcgtgacaag ccagatgttc gcaggaacag ataaggcaac gaatgcaaaa atgattgcta
    25021 cagttacaag cttagttgta ggtatccgct tagttgaaat tatttttgat cctttgattg
    25081 gtggcatcat cgataatact cgtacaaaat acggtaaatt caaaccatgg ttagttatcg
    25141 gcggcgttgt tagttccgta ctcttggtag caatctttac caatttcttt ggcttagcta
    25201 ctcataatac gactttattt atcatattat ttgcaatcgt tttcatctta ttggatagtt
    25261 tttattcatt taaggatatc gctttttggt caatgattcc agctttgagt acggaaacaa
    25321 aggaacgtga aaaatttgct acgtttgctc gtttcggctc ttctctaggt gcaaatggta
    25381 ctactctagt cgttgtccct atcgttactt tctttaccta tcttgttact cataaacata
    25441 ttgaaggcgc ttcaggctgg ttctggttcg gtgtgattgc tgctgttatt tcaggcggta
    25501 ctgcgattat cactgccttg ggcacaaaag aagtcgatac tgcgatccgt cagcaaaatg
    25561 aaaaagctgg gatcaaagat gtgttcaaag cagttgctca aaatgatcag ttgatgtggc
    25621 ttgcccttgc ttatatcgct tatgcgattg ccaatgcagc aacaactgcc gtattattct
    25681 acttcttcaa atatgtactt ggccaaccaa ctgaattttg gatcgttggt ctagttgctg
    25741 ccttacttgg tttagtagct gttccactat tcccaatgct tacacgcgtc atctctcgtc
    25801 gctacgttta ccttggaggg atcgcaatga tgatttcttc ttactttttc tttacgatcg
    25861 caggttcaag tttgccaatc gttatcatcg ggctagtatt gttctacttc cctcaacaat
    25921 tgattttctt atccgctttg atgacgatca cagactctgt tgaatatggt caatggaaaa
    25981 atggtatccg taacgaagct gttactctat caattcgccc tttactagat aaaatcgctg
    26041 gtgctttgtc taacgggatc gtagggtttg tcgctatcgc ttctgggatg actggtaatg
    26101 ctactgctgc tgatatcact gctcatggcg taacgacttt caaagcttat gcattctatg
    26161 ctcctgctgc tttgatgatt ttctccgcta ttatttttgc ttggaagatt acattgacag
    26221 agaaaaaaca tgcacaaatc gttgagaaat tggaaaacaa attggctcca gctgatacaa
    26281 tggaagatga acttcacgat gcagtagttc cttcccatta agaaaggatc ttgaaaatga
    26341 agattacaga aaaagatttt ggccaaggtt atcacttgat aaccttggag aacaaaaaca
    26401 agctagcact ttctatctct gatttaggtg ctagaatcgt cagtttaaaa tcaaatgatc
    26461 gagaattagt tttgggattt gacaccgctg aagaatatat cgaaaaagat ccgtatatcg
    26521 gtgcttctat cggtcgcacc gcagggagaa tcgaaaatgg ccacttttct ctcaatggaa
    26581 aaacttacca attagccaca gatccaaaaa ctggacataa tctgcatggt ggtgctcctg
    26641 gatttgaatt aaaaaaatgg tcttatgtca ttttgaatgg cgaaaatgaa gcttctgtca
    26701 tttttactac tacaagtccc gacggagaac atggttttcc aggtacaatg gatgtggaaa
    26761 tccgctatac tttgacaaaa gataatatct ggcgagtgac ctctcgcggc accagtgacc
    26821 aagatacttt attcaatccg acaaatcatg tttacttcaa cttgacggga gatgcttctc
    26881 agtcaatcga tcaacatgag ctttggctca atagcgaagc ttatgctcct ttgcgaacag
    26941 atagtattcc tataggggta aaagaaaatg ctgcaggaag tgctttcgat tttcaaatac
    27001 ctaaaaaatt agcgtccgtt tttgctagtg atttggatca aaaaaatctt gttgacggaa
    27061 tcgatcatcc atttttctta aaagaaaccg gcttgggtaa agaagccgct cgcttgacta
    27121 gccccgataa aagaatccaa gtagatattg ctacagatgc ttctagtgtg gttattttca
    27181 ctgcaaattt cggcacagaa acaccagaaa tgcgcaaccg aaaactggca catcacggtg
    27241 gcatcacatt tgagactcaa acagctccag gtgcagagcg cttctcagca tttggttcga
    27301 ttcatttaaa agccggatca gtatttgaaa cagtgacaga attcaaaatc aaaacaagaa
    27361 aggaataact catcatggaa atcaaaagaa gcttaaacac aaaatttgcc gaaacattcg
    27421 gacctaaaaa aactacacaa tacttctcac caggacgcat caatttgatt ggtgaacaca
    27481 cagattacaa tggaggccat gtcttccccg cttccatcac ttatggaacg caaggtgtag
    27541 cagcacctcg agacgataat aaaatattag tttattccac aaactttgac gatatcggtg
    27601 tcattagttt tactttagat gagctagaat atgataaaaa agcaaactgg gcaaattacg
    27661 taaaaggaat ggtccttaaa ttaaaagaag ctggacactc aatcgatcat gggtttgagt
    27721 tattagtgga aggaacaatc cctaatggtg caggactttc cagtagcgct tcattagaat
    27781 tattagtagg tgttgtatta gaagatctgt ttgatttaaa aacagatcgc ttggaactag
    27841 tccaaacagg taaaaaagta gaaaatgaat tcatcggtgt caattcaggt atcatggatc
    27901 agtttgccat cggcttcggt gagctagatc atgcaatctt attagataca aatacgctga
    27961 aatacgaaat ggttccagtg aaactagatg gttatgcaat cgtcatcatg aatacgaata
    28021 agcggcggga attggcagat tcaaaataca atgaacgccg tagcgaatgc gaagaagcct
    28081 tggcacgttt gcaaaaaact ttgcctatct cttctttagg agacttagat gaagaagaat
    28141 ttgaatcaac tattgaccaa atcggcgatg atacattgat acgcagagct cgccatgccg
    28201 tttctgaaaa tcagcggact ttgaaggcaa aagctgaatt agaagctggg aacttagaag
    28261 catttggtca actgttaaat gattctcacc actctcttcg ctatgattat gaagtaactg
    28321 ggatcgaatt agatacacta gtagatgctg ctcaaaaaca agaaggtgtc ttaggtgcac
    28381 gaatgacagg agctggattt ggtggctgtg cgattgctct tgtcaaagaa gaaaatatac
    28441 ctgccttcaa aaacaatgtc tatgatgaat acatgaaagt tgtcggctat gctccggaat
    28501 tttatgttgc acacattgga aatgggacaa caaagatcga tccagaagct gttcatgttt
    28561 gataaaaaaa tcaacgatct cagctaaaaa caaatagata aaaataacct ctcagcaatt
    28621 tccaaatgtg agccttttgc tcgtatttga aaattgctgt tttttattga cacggaaaga
    28681 gaacgtgtgt tctaattgtg ttgaagaggt gattgttatg tgggcgaatc aaaatccggt
    28741 atttgtttat gaaaaagaac cttcaagaga tattttatgt attgactgca aatcttttta
    28801 cgcctccgta gagtgcgttg aaaaggatct tgatcccctt acaactaaat tagtagtcat
    28861 gtcctatccg tccgattcga cagaaacacg gggtagcgga ttaatcttgg cttccagtcc
    28921 tgcagcaaaa aaagcttacg gcatcaccaa tatcagccgt gccagagatc ttcccttccc
    28981 ctatccaagt gatttatata ttgttgcccc aaggatggcc tattatatgg aaaagaacag
    29041 agaaatcaat tctatctata aaaaatatgc agacgaagcg aaccatcatg tctattccgt
    29101 cgatgaaagc ttccttgatg taacaaatag catggcactc tttggtaaaa caaatgcgta
    29161 cgacatggct aaactgatcc ataaagatgt gtttgagcag accggaatct acacgactat
    29221 aggaattggc gataacccgc tacttgctaa actagcctta gacaatggct caaaagacgg
    29281 aaagcaaatg tttgctgaat ggcgttatga agatgtacca gaaaagttat ggaaaatctc
    29341 agatatcact gacttttggg gaatcggcaa acgaacagca aaacgtctga aacaaatggg
    29401 tatacattct gtttatgatt tagctcacgc ggattattat cacttgaaaa gcaagctagg
    29461 ggtcatcggt actcagcttt atgcccatgc ttggggcatc gatcgaagtt ttttgggaca
    29521 agaatataca ccaaaatcaa aaagtatcgg aaatagtcaa atcctagata gagattatac
    29581 aagacgagaa gaaatcgaga tcgttatcaa agaaatggcc gaccaagtcg gcactcgctt
    29641 gagacgatct gcagcaaaaa cacaagtagt cagcttatgg gtaggatact ctttaggcta
    29701 tcttgatcca aatggcaaga ccgggtttca ccagcaaatg aaaatcgaag cgacaaatgc
    29761 tagcagagat ctagcagatg ctttattgac cattttccac cagcattacc actaccagga
    29821 tattcggcgt gtaggcgtga actgttccga cctcgtatac gcgacaggtt tacagcttaa
    29881 cctttttgaa gatcctgaaa agcagataaa tgaagcgaaa gttgattttg ttgtcgatac
    29941 gattagaaaa aaatacggct ttcaatcaat catccatgcg catagtctct tgcaaggagg
    30001 acgcgccatc gcacgtagtt ctctcgttgg cgggcatgca ggtggtatga gtggattaga
    30061 aggtggaaaa gatgagacgg acagcaaaaa aattcattga ctataacgag tatcgagatc
    30121 ggccatttgg attaaaatgg gggactgctt ttgcgatgga agaactagtc aacggtatca
    30181 aaaaaaatga agaagaagca cgcaaacagc ctcttcctaa acaacaaatg acattgcaag
    30241 aaatcgatag tatactgaca gaagcctttt tatctcgtaa agcagtaatc attcagctca
    30301 atttaaaaga tgaatttgga agactcttgg atgaggtcga agggcacttc attggtgaag
    30361 cctatgaaga ttactttatg attgaccatc agccgatatt gtgggaagac gtacggtata
    30421 ttgagttaaa ggaaacaaaa aaatggtttg tgacatgaga ggttgtgaca gaagtgatca
    30481 acttcaaaaa ataagaaagg atttttcgaa aattgcttct taaatttttg tggaattttg
    30541 gcttattttt cgaagaagca acttctggaa caacgtatat tagatatcag aggttgtgaa
    30601 aaagtcgctt agttttgagt aacaagccaa ggcgctgatt tcacaacctc tgtctatcta
    30661 tccatctatt cggtaagatt catgcatgac gtcttcttta tttagtatgt agagcccttt
    30721 ttcttctgac cattcaacat aaatcagctg ttttacatct gcatagcctt ctttcttcaa
    30781 gttctccata agccatgctt cagtcaattg attggactga agcactttat gctgaatttg
    30841 cccttcatct acaagcagga tcgataaagc tttgtcttca tctgattttt tcaatacact
    30901 catttgaccg gcattctcta ggataacatg tttggcattt tcaagaaaaa agcattcttg
    30961 ctgtcttaag attgctcgca actgctccat ctcgatatgg ttatttgtca actctgtcat
    31021 gttgatgacg ccgtctttga ttagaacaga tggctcgcct ttgacccaac gattgacctt
    31081 ttcatttttt tggacgatac gttcgatacc ataaatcatt actgcccaaa gcgcaatggc
    31141 aaataggaca tgccctacat ggacattgtc atcataggtt gattcttcta agatcccacc
    31201 taaaacaagc gtatagacta aatcaaacgg tgtgatttct gacatggatt tcttcccaag
    31261 caaacgtacc actaataata aaccgatcaa cccgaacacc agtttcattg taattgaaaa
    31321 aatcattttt tctcccccta actatctgtt aattaaatta tatatgcttt ctcacgaaca
    31381 cgcatggaat atacaaggaa gaaatgtatc cttttactaa aaaaaggact gtgacactgt
    31441 agtgaccccc aaaagttaga cttttttgga gtggagaaat ccactccttt tttttatgct
    31501 ctaaaatgat atttttacac aaccgttacg gttgttttac gaaattggac gagactttgc
    31561 cagtttaatt tctgttttat ccgttcatta ttgtagtaat agatatagct atcgatggct
    31621 gattttaatt cctcgaaaca gttataaact ttcccgtgaa aaatctcttg ttttaataag
    31681 ccaaaaaaat tctccataag tgaattatct aagcagttcc ctttccgaga catgctttga
    31741 aagattttgt actccttcaa tttgtttcta taagctttca tttgatacgc ccaaccttga
    31801 tctgagtgaa acgtacgacg atagggacaa tcgttagtgg tctggatagc ttcctccaaa
    31861 ccttccatta ccgctaatgc attgggcttt tcagagatac gataggataa aatctcacta
    31921 ttgaacatat ccataaaagg atctagatac aatttctttt gacggataat gcctttggca
    31981 tccacttcat ggtatttaaa ttcagttgta tccgtcgtga ttttctgatg gcagatactg
    32041 gtgtagaaac gacgatggat ccgatttttg gctactgctc caacttttcc tcgataagaa
    32101 ttgtagcgac gtgatttacg cgtgtatgcc gttactttta ttcctagctt cttgatcaag
    32161 cgctgaactt tctttttatt gatagagaaa ccgcggtttc ttagttcatt ggtcatccgt
    32221 aaacagccat aatccttgtg cttctcccga attttaagca tttccgcttc aatctcttga
    32281 tctggatttt tacggtccaa tcgcctttgc catccaaata atcatgaacg atctttaatt
    32341 taagttcaaa agtatattta accataaaaa ataccgacct ccaaaagtta gattttttgg
    32401 tctaactttt gggggtcggt acacaaatcg tgtcacagtc ccttttttga aaataaacgg
    32461 tgttcaaaag cttgcatctg cggtaataag tccagccaag ttaaaaacat aagaagctgt
    32521 ttttacttgg ctgttcttat tacttgatgc aaggcagctt tttcacaacc tcttttcaaa
    32581 tatttcgttt tcttattcta caaatggttc ttctcctaac gcttgttgag cggctagatt
    32641 caacatgctc cattgtcggt caaagcctgg ctggaagaag aagtcagctt ctgctagttc
    32701 ttctaaagtc agtttgtttt ggatcgctag tgccaataca tttccttgac ctgtaacatc
    32761 ataggtagat aatactgctc ctcctagcaa ttgatgggtc attggatccg agaataattg
    32821 gatggacact tttggatttc cttgttcttc tggtacatat tttggtcgta acgtatcttc
    32881 ataataagac actcgtacat ctactcctgc ttttgctgct gtaaagctat tcaatccgct
    32941 tgctgcaaaa tgatagtcaa agacacttaa tgcagaggaa cctacgactc cgccgaacgg
    33001 tttgtttggt acttcttcaa aaagatgttt tactacataa cgtgcttctc tacgtgcaac
    33061 agtcgctaaa gcgatcggca tttttttgcg tgctgggata gaataagcaa gtgtagcatc
    33121 tcctactgca tacacatctg gaagattcgt ttgcaagtat tcatttgttt ggatccagcc
    33181 acgttcatct aagtcgacga tcccttttaa ccattctgta tttgccttca caccggcagc
    33241 ttgaatgact agatctgctg ggatttcttt ttcacttgtt ttgattgcct caacttttcc
    33301 gttaccagta aaagcttcga tttttgcatt tgtcaaaact tcaataccgt tttcatttag
    33361 gtgttgttct aaaatatctg tcatctcagc atctaaataa gtgcctaaag gtctatcgat
    33421 cacatcaagc aatacgactt ctttcccagc ttttcggctt gcttctgctg cttcgatccc
    33481 aatgtaaccc gcacctacga cagcgatttt tttaatagcc ggatcattta atttttcttt
    33541 gattttggtt gcccaattgt atccacgcat caagtagaca ttttctagat ctgctcctgg
    33601 tactggcaga ttgtttggtt tgacaccaga actcaaaatc aatttgtcat aaggatattc
    33661 ttttgtttcg ttatctgatg cgctagtaac cgttactgtt tttttatcgg catcgatcgt
    33721 ttttacttcg tgattattta aaatatgaac attggattgc ggaaagtttt ctggtctgaa
    33781 attacgtaca tcatttacat ctgttacctt gtcttctaaa tacagctcca taccacatga
    33841 catgaaggaa atatagtctc ctgcttcaaa tagcgtgata tctacatttt caccgtatcg
    33901 tgtcaatagt tccaagatcg attgatgccc tccatgagat gcacctacaa tgacaacttt
    33961 gattttttcc attgatgaaa cctccttgaa tattctttta aaatcattct aaacaattta
    34021 aacttaacat tacattagtt atgaatcaaa agaaacgcgt tcatttgaac taaatttaaa
    34081 aaacaatcgt ctccttgcag aatataccgc aaaattaacg attgtttttt tgataaaatc
    34141 tatgaatcac tgttctgtta aaaagtcttt gatccgtttg caggcctcat gtaatttttc
    34201 catgctgcta gcataagaaa tccggatata accttctcct tctgggccaa atgcaattcc
    34261 tgggatacac gcaacttttg cctgttccgc taattctaca caaaactgcc aagagtcagt
    34321 ttgaatggca gctgggagtt tgcaaaacaa gtaaaatgca ccgtttggct gggtcacttc
    34381 aaatcctaaa ggcgccaatt gctggaccag atagtctctt cgtgtttgat attgctcttt
    34441 catattctcg ctatctttca ccccatttat tagcgcttca gccgcagctt tttgcgacat
    34501 cgttgtagcg gcagtcacca gatattgatg gactttaatt agttcgtcaa taagggcttt
    34561 ttgagcaaaa ataaagccca ctcgccaacc agtcatcgca tgtgacttgg ataacccgct
    34621 tatcacgatt gtttgttctg gaagataagt agctatggac atatgcgttc cactataagt
    34681 caattcagca tatacttcat cacttataac aaataccgga tattttttta aaacctcagc
    34741 gattgctttc aattgttttg cagacaatgt cgtcccagta ggattgttag gatagtttaa
    34801 aatgactgct ttgacagctg atccatattg ttcaaatgct cgttcaaatt gttccggctg
    34861 acagacaaac cctgtgtcag aagtatctat cgggatcaat tcagcaccag ctagttccac
    34921 taatggctga tatcctgaat aagcaggcgc tggaataaga accttgtctc cttcttccaa
    34981 aacagataac aacgcagagg caatcgcttc cgttgcacca actgtcgtca aaacttccgt
    35041 ttgcggatca taagttaatc cataacgctg ttgctgaaat atacaagccg cttcccgcag
    35101 ttcctctaat cctctcattc cggtatagtg agaaaagtct tcttcaatcg ctgctattcc
    35161 tgcttgcttg acatgttcag gagttggaaa atccggttct cccagggtga gtttgatcac
    35221 atctggtatc gacgatattt gctgatcgaa ttgtcgtatt tttgatactt ctattctaga
    35281 aagcctagga ctaaatcgat tggataaatt catcattatt tactcgctcc tcttttgaac
    35341 tgctatcgat ttttacaaga agattttctg taaaaagaat cgttttgtac cattctacac
    35401 cattttctaa aacatccata tcaaaatcga acgtcggatc atgtaaagca ggggtatcac
    35461 cgacacctaa aaagaaaaat acacatggat attgttgcgt atatatgcca aaatcttcgg
    35521 cttgaagaac tggagcatct aattcatgta gtttgtttga atctaacagt gctgcatata
    35581 agcctctatg gttgattaca gctggatagc cgtcattata gcgaaaagaa atagtagctg
    35641 tagagtgaaa ctgttctgct aaatggtcta atcccctctt caaataattt tgcatatctg
    35701 aactgtaaga acggatactt ccggatacaa taacttctcc agctaaaaca tttcggatcg
    35761 ttccaccttg gatttggcca aatttcatta gatgattcca ctctgccggt aattgccggt
    35821 caaaatcata aacattcttc aaaaatcgta cagctgcctc cagactatcg attccttttt
    35881 cactagaagc aatatgtgca gactggcctt taatgataat gtctgtttca ctgctttgtg
    35941 ccatcaaagg tcctggtcga gagaaaactt ctcccttagc tagtccaggc catagatgaa
    36001 agccaaatat tgcttgtact tcatatttat ccaacaatcc tgactgaata acgctttgtg
    36061 cgccaccgga aatttcttct gatggctgat agatcagtac tacattataa ataagatcct
    36121 caatgtgttt agttataaag tcagtcaacc ctaaaagcat cgccatatgt ccgtcatgac
    36181 cacatgcatg catataacct tcatgcttag aagcaaaaga gagattcgtt tcttctttga
    36241 ctggcagtgc atcgatatct gttcgaaaag caatcgtttc ttcttgctga ttatcaaaat
    36301 aaaggagtag tcctgtttcg ccgatttcat ggataacggc ctcgctgttt tttacttgtt
    36361 catataaata tttttttgtc ttgaattcat gaaacccgat ttcaggaatt tgatggagtg
    36421 ccattcgata ttctttcagc atccgtattc accatccctt catagatacc cttcactact
    36481 gtttcatatt gattattcgg tacaccgatg atcagattca actcctgtgc tccttgtgat
    36541 aagatcgatg tgcgaatttc taatttgttc aatatcgata acactttccc agataatcca
    36601 atcaattctt tatgcggacc cccaacgaca gagatcaaag ctaagtcttc gatcacgacg
    36661 atttcttcca cgccaagtgt ttcttttaat tctttcgtga tgagaaataa cttatcggca
    36721 atcgcttctg ctgagacgat cactccgata ttgtcgatcc ctgaaggaat atgttcgatt
    36781 gatatctcat gtttagcaaa aatgctcatt gctttccaga taaacccgat ttcatcggac
    36841 atatgacgtt tgaataaagt gatggaaaga aaatccttgc gtccagctat tcctgtcagt
    36901 ccattttcct tttctatttc gtgattcgat atttttgtcc cttgagcgaa aggatcattg
    36961 gtatttttta tttggatggg gatatcagcc tcttgaactg ggaaaacagc tgcttcatga
    37021 agaacgttag cccccatata agccatctct ctcagttctc taaaagagat ttcctcgatg
    37081 attttaggat gatcgatgat tcgcggatca gccatcatga ttcctgaaac atccgtccaa
    37141 ttctcataac tttccgcttg caacatcttc gccagtatcg cgccggtaat gtcgcttccc
    37201 ccacggccca ataatttttc ttttcctgca gggttcgccc cgtaaaaccc tggaacaacc
    37261 attttcggat atcttttaaa tgcttttgtg atttcctgtg ctgttttttc ctgatcgatt
    37321 ttcccatcat agtcaaagaa aatcaggtct tttgcgtcaa taaactggta accaagatac
    37381 tcagccatca gttgagcagt cagatattca cctctactta ctaaataatc ttcagttatc
    37441 ttttcgtctt ccagttgttt ctgtatgcgt gctagttcat cttcaatagg taactgcagg
    37501 gcaagaacat ctcttacttg aatgaaacga tcacaaatcc ttttccaaag gggtgtccaa
    37561 tcaacatgat gctgaaggta agcataaatc aaataaagta agtctgttac tttgtcatca
    37621 tctgacgtac gtttgcctaa agcagaaaca acgatcactt ggcgattggg atctgaaaga
    37681 acaattgtac ggactttttt gaattgctct tcatgagaca tcgatgttcc gccgaatttc
    37741 gctactttca gcgtattact catcttgcca cctccgctat tttctgcatt aaagctgcag
    37801 caatctgtac ggcattcgca gcggctcctt tgcgtatgtt atcggcagtc gtatagaaca
    37861 acaatccgtt ctctaaagaa gtgtcttttc tgatcctccc aacataaacc atatctgttc
    37921 ctctagcaag aatgctcgtc ggatatttat gtgctgctag atcatcttct aattgaatcc
    37981 ctggaaactg gctgagacac tctctaattt cttccgtcgt aaactctttt tgcagttgaa
    38041 ctgccacaga gactccatgt ccattttcga ttggcacacg tacgcaggtt gctgatactg
    38101 gcaaagcagg ctgatgcaga atcttacggg tctcatttat cattttctgt tcttcttttg
    38161 tatacccatt ttccaaaggc tgatcgattt ccggaatcac cgtttggcta atattataag
    38221 gatacaaaac tggttcctcg tcattttgcg tacgatgaag atcttcaatg cctttttgcc
    38281 cggaaccaga aactgcttga taggtcgaat aattgactcg ctggatacca aaggcttctt
    38341 gtaaagcttt aagcggaagt accgcttgaa tggttgaaca atttggattt gcaatcagcc
    38401 gattcaatgt atagtcttcc agattgacct ctggaacgat taatggaata tccgaatctt
    38461 cccgccatgc tgaggaatta tctatgacaa atagtccctg acttcgtgcg attggcgcaa
    38521 actctttgga tacagaagca cctgcagaaa atagcgctaa atcatatcct tcaaatgaag
    38581 tttctgtcaa ttcctgtaca gtgtgtttct ttttcttgaa tacacaaact ttaccggctg
    38641 aacgttttga agctaataat ttcaatcgtc ctactggaaa atcatattct tctaacactt
    38701 tgagcatcgt ttggccgact aatccggttg cccccacaat acaggtatct atttcgcgca
    38761 tttttctcca tctcctctat aattcgcgta acgcagcaat caatgctgta ttttcttttg
    38821 tctgatcatc acttattttt aatacacgtg caggtattcc accgacaaca gtttctgctg
    38881 ccacatcttc taaaaccact gctcctgctg ccacgactgc atcttttccg atatggacac
    38941 cttcaacaat cactgcattc gcacctacca gtacgccgtc ttctacgata accggtttgg
    39001 ccgatgctgg ttcgatcaca cctgctaaga cagctcctgc accaatatgg cagtttttgc
    39061 ccactgtcgc tcgtccacct aagacagcac ccatatcaat catcgtgttt tcaccgatta
    39121 ctgcaccaat attgataatc gctcccatca tgatcacggc attgttgccg attgacactt
    39181 gatctctgat aatagcacct ggttcgatac gagcattaat ttttttaata tctagcaaag
    39241 gaactgcgct gtatcttgca gcagtttcga tttcgatttc aatgattttt tctgattcag
    39301 cttctaaaac cggagcaatc gtctcatagt caccaaaaac aacgaagctg cttccttcac
    39361 caaaaacttt actttcttta aattcgattg gctcggttac attcagatag actttcacag
    39421 gcgtcttttt agatgccgtt tgaatgtagc ggatgatttc ttgagcttcc acgtttttta
    39481 cacccctttg tccattaata aatcttctaa tttgtaataa cctggtgact gatctttcaa
    39541 ccagtccgct gcttctactg cgcctgcagc aaagattcga ttagaaaaag attcatgttt
    39601 gatcgtaaat atttcatcag tagcagcaaa caatacatca tgttcaccag gtaaagaacc
    39661 tgcacgaata ctgtgtacac caatttggtt ttcttctctt tttttccctt gccagttgta
    39721 tacgggctgc agcttttgca cttcttggat tgtttgaagc agcatatttg cagtaccaga
    39781 tggtgcatct tttttacgac tatgatgttt ttcaatcagt tcgatctgcc agccttgcaa
    39841 cattgcagct gcttgcttga ttagttgatt catcaccatg atgccgaacg aaaaattagc
    39901 actatacatc acaggtacat gtttggcatg ttcttttatt tcttggaact gtgattgtgt
    39961 ataacctgtc gtgccaatga ctaacggaag ttgatatgat aagctatacg aaaggatttt
    40021 ctctaaatta tctggatgag aaaaatcaat aagcagttct ggcagttccg taaaagcaag
    40081 cgtagttgct tctttaggtg ccgtccgtaa aagaaggacc gtatctcctc tttcttgtgc
    40141 aacttcttcg actctatttc ccatctgtcc actgccaatc agtccgattt tcatttttca
    40201 accgtccttt cttttaacaa aagctgagct aattgtttct ttttctcttt tgaaagagaa
    40261 actaaaggca ggcgtagagg tccggaaggt aagtctaata atgacatggc tgctttgaca
    40321 ggaataggat tggtctcact gaacaaggca tggatcagtt caagtgcatt taattgcttt
    40381 tcttttgctt gctgccatct tccgtcctgc gtatctttta ctaattcatg gactatttta
    40441 ggttggatat tcgcccatac acttattaca ccgcttcctc ctacactcat tactggtagg
    40501 atcaaatcat catttccact gtaaaggaag aattcttctc caatcaaccg agcggcatcc
    40561 atcacataag ccatatctcc tgaggcttct ttcaaaccaa tgatattagg atgttctgct
    40621 aaatttacta atacatggat tggaatcgac atgcctgttc tagaaggaac attatacaaa
    40681 ataagcggaa tcggtgaggc ttctgcaatt gctgtaaaat gagcagctag tcctgcatca
    40741 cttgttttgt tgtagtaagg ggtgatgacc aaaagtgcgt ctgcccccaa agaagcaaaa
    40801 tgcttcgctt tttcaatcgt ttctttcgta ttattcgttc cagctcctgc aatcaccggc
    40861 acacggccag ccactttctt tactgtcagt tgaaggatct gctcctcctc agattgtgtg
    40921 agcgtagaag attctcctgt tgtacctaaa actaataatc catctgtttg ttcatctaaa
    40981 tggaattgga tcaatttttc caaaccagca taatctactg atccctcttc attcatcggc
    41041 gtgatcagcg ccacgatcga accttttatc atcttcattc ctccaatatt cttgtccatt
    41101 catgaactgc tgggccagcc atttgtatcg catcctcacc agagatcatc aagtctcctt
    41161 gttccaagtg aacaactacc tgatctgctg aaattttccc agtttcctta gcaaccacat
    41221 aagaggcaca gcaacctgta ccgcaagcaa gtgtccaacc tactcctctt tcataggtac
    41281 ggacgatcaa ttcttgttca ttgactactt ctacaaagtt gacattggtt ttttctttga
    41341 acaaaggatg atgacagatc agttcacctc tttttctcca gacttcctca cgcgcatttt
    41401 caacaaaaac aacggtatga atcgttccta aaaataaact agtgatcgtt actttttcac
    41461 cattgatctc tatttcctgg ttttttattg gataagtttg attagctttg attttttccg
    41521 gagaatcatc tggcactccc atatctacgc tgcaataaaa aggtgaggga tccaacaccg
    41581 taatttttct tgtgcctgct aatgtctcta cgttgaattc ctgttcattc atcatgttct
    41641 gatcatttac gtatcgagca aaacagcgaa tgccatttcc gcacatcggc gcgcggctgc
    41701 catctttgtt gtaatagatc atttccaacg gatcgatttt tacggcgatc aatccgtcta
    41761 tatcatattt ttcttttgtg cataaagaag cggctaattg actataatct gtgtcttctg
    41821 cgtaatctaa taagataaaa tcatttccgc agccattata tttatgaagg atcataggaa
    41881 acttcccccc ttagtagatc agaaaatgct tgtctgcgaa caatcagtct tgattggcca
    41941 ttattgacaa aaacaactgc tggtcgtgtc gctcgattgt agttgctgct catcgagtat
    42001 ccataagctc ccgtggaata aacagctaaa agatcacccg attctgcttt cggcaaataa
    42061 gcttctttaa ttaccacatc gcctgattca cacatttttc ctgcaatcgt cactttctct
    42121 gttttttctt ccgacagttt tgctgctaaa tcacaatcgt attctgcctg atacagagct
    42181 ggacggatat tatctgtcat cccgccatct acaaagtaat attttttatg tggtgtcttt
    42241 ttgatgaaac caatcgtgta tagtgttgta ccagcttctc caacaatact tctacccggt
    42301 tcgatcagta attgacgaat ggtcagtttt cgtttttcca aagcttcttc cgtatattca
    42361 atcagttttt ttagtgcctc ttcgactggg atcggctgat cattttgtgt ataacgaatc
    42421 ccaaaccctc cgccaagatt caaggaaaga ggttggtcaa aatcggttaa gtaattcgtc
    42481 aatttgtcga tttcagctaa ccaagcagta acatcaaaaa tttgtgaccc gatatgagca
    42541 tgaaatcctt ctaactctag atagctgctt tcttttatca actgaagggc ttgttggcac
    42601 tctttacttt catagctcat gccgaatttt gaatcaatat gcgcagtgac gatatattca
    42661 tgagtatgtg cttctactcc gacattcaac cgaagcatga tacaaatttt ttttaaatag
    42721 cgttggctta attctgctaa taattcaact tccatcaaat tatcggcaac aatatgcaac
    42781 aaatcatttt caagtgcata gatcagttcc tctggcgtct tgttgttacc atggaagtag
    42841 atagtatctc taggaaaatc agcttgcaaa gcggtataga tttccccacc actgacaaca
    42901 tctagcccga atccatattc acgaactaaa tgcagcatct caatcgtttg gaatgcttta
    42961 gaggcgtaaa gcacttttgt atcaaaacgt ttcgatacaa agcctcgctt gaatgacagc
    43021 atcgtttgtc tcatcttttc ttcatcatac acatacagag gtgtcttata cttttctgcc
    43081 aatgtgcttg ctgtgactcc gccaattatc aattctgtca ttttttcatt cctcttttca
    43141 ctttttttcc gtttttattg gaatttcatt tgataaagat ttgaatttta ttggaataat
    43201 attaaacaaa attttctgaa ttgtctagta tatttcgaaa tattttttta aaaataagaa
    43261 taaaaatggt tataaaaaca aaatacccga actattagaa gctatttata cataaaaaca
    43321 taaattcaac actatttagt gatgaaaaaa tattttcgct tcaatatagt tcggaatagt
    43381 tcggatattt tcaattttat gaatcatttc aatttcttca taaataagtg cagttttttc
    43441 tttctctagc ttttgccatt catctttcaa aatataaata aaatcaaata cttttttatt
    43501 tttcaatttg ctgattccac aaaaaacctc ttgaaaggca gatccgatga tctgcctttc
    43561 aagaggtcaa gctctgtttt caaattctta tcaatccgtt agcgaattat tttgacgcac
    43621 gttttacata agaaccttct gctgtattga tttccaacaa ttcatctgtt tcgatgaagt
    43681 caggaacatt gactactaag ccagtttcca ttgtcgctgg cttacctgaa ccagtgactg
    43741 ttgcaccttt gatggaaggt tgtgtttcca caacacgtaa tacgacagtt gttggtaatt
    43801 gaacgccgat gacttcgcta ccgaaaaatt ggatttttac ttccatgttt tctaggatat
    43861 atttcatttc ttcttctact gtttctacag gaatttcata ttgctcatat gtttccaaat
    43921 ccatgaagaa agctgtatca tccatcgtgt acaaatattg tacaggtttt gtgtcgatat
    43981 gtgctttttc aaatttttct tctggtctga acgttgtgtc gtatgttgca cctgaacgga
    44041 catctttcag tttcatacgc ataactgtat ttcctttacc aggtttatgg tggcttgcat
    44101 ccagaacttt aatcagtttg ccgtcttgta cgaaagtcat accggctctt aaatcacttg
    44161 ctgagatcat tatttttgct ccttcttttc tttatcataa tcaactttat tgtatcagac
    44221 aaccggccag aaaactagag tattctctat cttttaaggt ctcatgccta tttgtcagaa
    44281 aaataaacac gggtaaacga tgctatcgaa atattttttt ctttcaagcc tttgactttt
    44341 attttttttc atgctatcgt taataaaaat tttacttgga gggatacaca tgacagtcag
    44401 caaccaaaaa caattgaatc gttgctgttg tatggtgcat aaaagcagca tactatttgt
    44461 catgtctatt aattgccctt catagaatga gtaccaggta acttatcttt ttatctactc
    44521 aatttttctc tatgtctttc aaggagctga tttaatagtc agctcctttt tttattatct
    44581 ttttttagga ggcccacatg attatctcga caattacaga agctattggt tccacaccgc
    44641 tttatcgctt tgacaatcag cactattcta tgcctaatgg ttctgctatt tatgcaaaac
    44701 tagaatattt aaatcctgga ggaagtatca aagatagatt gggcagttat ttgatcaaag
    44761 aaggatttgc ccaaggaaaa atcaatgaaa aaacgacgat catcgaacca actgccggaa
    44821 atacaggaat tgggatcgct ctggccgcac tcacatacaa attaagaacg gtttttgttg
    44881 ttccagagaa gtttagctta gaaaaacaac aattgatgaa agcattggga gcaaagatca
    44941 tccattctcc tagtgagcaa ggaatcatcg gtgccatcag caaaagtaaa aaacttgcag
    45001 aggagatatc aaacagctat ctgcctcttc aatttgaaaa taaagataat cctgctgctt
    45061 actatcatac gttagggcca gaaatctttc atgaaatcaa agaaaatatc catagttttg
    45121 tagcaggaat tgggagtggt ggaacctttg ctggaacttc tacctttttg aaagaaaaat
    45181 atcccgacat tcgaataatc ggtgtagaac cagagggctc tgtgttaaat ggtggtgatc
    45241 cagcccctca cgaaattgag ggtatcggtg tagaattcat tccgccgttt ctttctcccc
    45301 tttcaatcaa tcagatcgag acaatctctg atgtagaagg gtttaactat actaggcagt
    45361 tagcacgaga acaaggacta ttagttggaa gttcgagtgg tgcggctttt gcagcagcat
    45421 taagagaaat tcgtcgttta ccacctggtc atcgtgtcgt tacgattttt ccagacgcag
    45481 cagaccgcta tttatcaaaa aacatttact tataggagga aagaaacatg cacattcaaa
    45541 caaaattgat ccacggaggc atcagtgaag atcccacaac aggagctgtc agtgtaccta
    45601 tttatcaaac atccacttat cggcaagatg gcgtcggcca gcctaaacaa tatgaatatt
    45661 caagatctgg taatcctact cgttttgcat tggaagaact gattgctgat ttggaaggcg
    45721 gtgttcgagg attcgctttt tcttctggtc tttctgggat tcatgccgtt ttttctcttt
    45781 ttcaagctgg cgatcatatc ttattaggag atgatgtcta tggaggaaca ttccgtctat
    45841 ttgacaaagt tcttactaaa aatggtctcg agtacacgat catagatacg agtaatcttg
    45901 ataaaataga acaatccatc aaacccaata caaaagcact ttatcttgaa acacctagca
    45961 atccgcttct aaagattact gatttagaaa aaagtgccac tctggctcat caacatggat
    46021 tgatagttat tgctgacaat acatttgcta ctccttattt tcaaagacct ttagatctcg
    46081 gttctgatat cgttgtccat agcggtacca aatatctagg cggacacagc gatgtagtag
    46141 ctggtttagt gacttctaac cataaagatc tagccgatca gatcggattt taccaaaatg
    46201 ctatcggagc cgtacttggt ccccaagata gctggttact tcagcgcgga atcaagacac
    46261 taagtgttcg tatggaggaa catcaaaaaa atgcttttgt ggtagctgat tttctatttt
    46321 ctcatccagc agtagaaaag gtctactatc caggattacc cgatcatgaa ctccatggag
    46381 ttgctaagca gcagatgagc ggctttagcg gaatgatttc ttttacttta aaaaatgaag
    46441 aatctgctat tccatttgtt gaatcactcc aactatttac tttagcagaa agtttaggtg
    46501 gcgtagaaag tttagtggaa attccttctg tcatgactca tgcgtctatt ccaaaagaaa
    46561 aacgagaaga ggctggcata aaggacggct tgatccgtct ttctgtagga attgagtatg
    46621 gtcaggatct cataaacgat ttagctcagg catttgacag aatcaagaac tagaagtccc
    46681 aataaacgac ttagcagaaa aatctaaaaa atatttcaga ttgctacacc ttggaaacga
    46741 taagtgttag aatcaagtag aaagacgttt ttgtaaattt cactaaataa ttctttagtg
    46801 aaaagcatca aaacgaatga acaaacagat aggagtctta caaatgagaa tgaatgtcga
    46861 aagttttaat cttgaccata caaaagtcaa agcaccttat gtacgtgtag ctgatcgaaa
    46921 aacgggtgcg catggcgatg tcatcattaa atacgacgtc cgctttaaac aaccaaataa
    46981 agaacacatg gacatgccaa gtctacattc acttgagcac ttgactgccg aactaatccg
    47041 caatcacgca gattacatcg tagactggtc tccaatggga tgccaaacag gcttttactt
    47101 gactgtattg aatcatgaaa gctacgaaga cattttagat gttcttgaag caacaatgaa
    47161 agatgttctt gaagctactg aagtacctgc aagcaatgaa acacaatgtg gctgggctgc
    47221 cagccataca ctagaaggtg cacaagcttt ggctcgtgaa tttttagcaa aacgcgatga
    47281 atggtctgaa gtagaagcat agtttctaaa agaacgtaaa gatgagaagc aatatagcta
    47341 tcttatcttc gtattttatt aaatatttat tgtctgaaaa gagttctaaa cgggaaaaca
    47401 aatagctagt tttcagttta gcgctctttt tattatataa aatagaaagg agaaaacttt
    47461 atgtctattt ataattttga aactacttta gaagacggca ctgtctattc attgaaaaaa
    47521 tatgcaggca aaccgataat cattgttaac actgcaacaa aatgcggtct agcacctcaa
    47581 tttaaacaat tagaagaact ttaccaagct tatcagacac aaggtctggt tatcttaggc
    47641 tttccttcca atcaatttaa acaagagcta gattcagcga ctgaagcagc agaagcttgt
    47701 aaaaccactt atggcgttac cttccctatg catcaattga ttgacgtaaa tggagaaaat
    47761 acagatcctc tttttactta tcttaaaaat gaagctcctg gtactgtcac taaagcaata
    47821 aaatggaatt ttactaaatt tttgattgat agaaatggca aagtaatcga acgatttgca
    47881 ccacaaactt ctccattgaa aatgaccgct gcaattgaat ccgttcttta acttaataaa
    47941 aataaaaaaa gatgaactat ttctagttca tctttttgcc tttgcgtggc gacgtcctac
    48001 tctcacaagg ggcaacccct cactacaatc ggcgctaaga agcttaactt ctgtgttcgg
    48061 catggttaca ggtgtatcct tctcgctatc gccaccacac tgtggtgtta tcttttattg
    48121 agtaaatttt gttcactcaa aactggattt gaagtca
//
LOCUS       DACEQH010000018        47861 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.018, whole genome shotgun sequence.
ACCESSION   DACEQH010000018 DACEQH010000000
VERSION     DACEQH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.018"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(37..1188)
                     /locus_tag="IW833_001046"
     CDS             complement(37..1188)
                     /locus_tag="IW833_001046"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ1307178.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1201..1638)
                     /locus_tag="IW833_001047"
     CDS             complement(1201..1638)
                     /locus_tag="IW833_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1307179.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1653..3419)
                     /locus_tag="IW833_001048"
     CDS             complement(1653..3419)
                     /locus_tag="IW833_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ1307180.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3683..4360
                     /locus_tag="IW833_001049"
     CDS             3683..4360
                     /locus_tag="IW833_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1307181.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4363..5490
                     /locus_tag="IW833_001050"
     CDS             4363..5490
                     /locus_tag="IW833_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1307182.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5690..6130)
                     /locus_tag="IW833_001051"
     CDS             complement(5690..6130)
                     /locus_tag="IW833_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1307183.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6141..8174)
                     /locus_tag="IW833_001052"
     CDS             complement(6141..8174)
                     /locus_tag="IW833_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1307184.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8176..9879)
                     /locus_tag="IW833_001053"
     CDS             complement(8176..9879)
                     /locus_tag="IW833_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1307185.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9973..10923)
                     /gene="manA"
                     /locus_tag="IW833_001054"
     CDS             complement(9973..10923)
                     /gene="manA"
                     /locus_tag="IW833_001054"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ1307186.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10962..11987)
                     /locus_tag="IW833_001055"
     CDS             complement(10962..11987)
                     /locus_tag="IW833_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1307187.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12196..13065)
                     /locus_tag="IW833_001056"
     CDS             complement(12196..13065)
                     /locus_tag="IW833_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ1307188.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13524..15416)
                     /locus_tag="IW833_001057"
     CDS             complement(13524..15416)
                     /locus_tag="IW833_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1307189.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15413..17140)
                     /locus_tag="IW833_001058"
     CDS             complement(15413..17140)
                     /locus_tag="IW833_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1307190.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17156..17806)
                     /locus_tag="IW833_001059"
     CDS             complement(17156..17806)
                     /locus_tag="IW833_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1307191.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18010..18306
                     /locus_tag="IW833_001060"
                     /pseudo
     CDS             18010..18306
                     /locus_tag="IW833_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            18478..19098
                     /locus_tag="IW833_001061"
     CDS             18478..19098
                     /locus_tag="IW833_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ1307192.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19190..20356)
                     /locus_tag="IW833_001062"
     CDS             complement(19190..20356)
                     /locus_tag="IW833_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1307193.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20368..20721)
                     /locus_tag="IW833_001063"
     CDS             complement(20368..20721)
                     /locus_tag="IW833_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1307194.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20849..21871
                     /locus_tag="IW833_001064"
     CDS             20849..21871
                     /locus_tag="IW833_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ1307195.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(22310..22744)
                     /locus_tag="IW833_001065"
     CDS             complement(22310..22744)
                     /locus_tag="IW833_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307196.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22854..24713)
                     /locus_tag="IW833_001066"
     CDS             complement(22854..24713)
                     /locus_tag="IW833_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1307197.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24831..25991)
                     /locus_tag="IW833_001067"
     CDS             complement(24831..25991)
                     /locus_tag="IW833_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ1307198.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26298..27722)
                     /gene="araA"
                     /locus_tag="IW833_001068"
     CDS             complement(26298..27722)
                     /gene="araA"
                     /locus_tag="IW833_001068"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ1307199.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27738..28436)
                     /locus_tag="IW833_001069"
     CDS             complement(27738..28436)
                     /locus_tag="IW833_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1307200.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28393..30054)
                     /locus_tag="IW833_001070"
     CDS             complement(28393..30054)
                     /locus_tag="IW833_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ1307201.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30272..31672
                     /locus_tag="IW833_001071"
     CDS             30272..31672
                     /locus_tag="IW833_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ1307202.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31924..33003)
                     /locus_tag="IW833_001072"
     CDS             complement(31924..33003)
                     /locus_tag="IW833_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1307203.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33089..34048)
                     /locus_tag="IW833_001073"
     CDS             complement(33089..34048)
                     /locus_tag="IW833_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ1307204.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34060..35583)
                     /locus_tag="IW833_001074"
     CDS             complement(34060..35583)
                     /locus_tag="IW833_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1307205.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35593..36156)
                     /locus_tag="IW833_001075"
     CDS             complement(35593..36156)
                     /locus_tag="IW833_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1307206.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36162..37025)
                     /locus_tag="IW833_001076"
     CDS             complement(36162..37025)
                     /locus_tag="IW833_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1307207.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37039..37935)
                     /locus_tag="IW833_001077"
     CDS             complement(37039..37935)
                     /locus_tag="IW833_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1307208.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38110..39381)
                     /locus_tag="IW833_001078"
     CDS             complement(38110..39381)
                     /locus_tag="IW833_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1307209.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39371..40888)
                     /locus_tag="IW833_001079"
     CDS             complement(39371..40888)
                     /locus_tag="IW833_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1307210.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41086..41976
                     /locus_tag="IW833_001080"
     CDS             41086..41976
                     /locus_tag="IW833_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ1307211.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="IW833_001081"
     CDS             complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="IW833_001081"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ1307212.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42885..44357)
                     /locus_tag="IW833_001082"
     CDS             complement(42885..44357)
                     /locus_tag="IW833_001082"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ1307213.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44994..45356)
                     /locus_tag="IW833_001083"
     CDS             complement(44994..45356)
                     /locus_tag="IW833_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1307214.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45579..46331
                     /locus_tag="IW833_001084"
     CDS             45579..46331
                     /locus_tag="IW833_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1307215.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46471..47394)
                     /locus_tag="IW833_001085"
     CDS             complement(46471..47394)
                     /locus_tag="IW833_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1307216.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47409..47681)
                     /locus_tag="IW833_001086"
     CDS             complement(47409..47681)
                     /locus_tag="IW833_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307217.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13933 a         9944 c         8260 g        15724 t
ORIGIN      
        1 aaaatttcgt cacattcccc tttttcgtat cctattttaa gcgacttgct tggcataacg
       61 gttcacgttt tgcttgatat tcccgattgt tttcggatca ttgattcctg tgacttcttg
      121 aatcaattgt tccaagtttt catggatctt catttcatgc aattgacggc tttgttcatc
      181 ttcaggatca tagtaattaa aaataatccc cattgttgcc gtcaaatgtg ggcaagtcaa
      241 gttatgttct tgtagttctc tcactggacg tgtaaatctt tcttgataac ctaacttacg
      301 taatggtgtc cgagcgacac gtgtgatcgc atcagagata tttttgtttt ggaagcgatg
      361 aatgattttt tcaatatacg catgatgctg ctctgcatca aacccccatt tggcaatcag
      421 caaacttcct gtttcctgca aaactgattt taattgtgcc acaactaacg catcttgcat
      481 tgcttcatca atggtctggt aacctaacaa ggcacctgta taagcgacag tcgcatgacc
      541 agtattgaca ctaaatagtt tgcgttcgat atatggttct agatcatcta catataaaac
      601 cccttttaat tggatttttt tattttttct ttggctatcg tcaatgaccc attctttgaa
      661 aggttccact tggacgaata aaggatcttc atggtgttgt aatgggacga ttcgatcgac
      721 tgctgcattt ggaaagccga tatatttgtc agtaaatgat gtttccggaa gatatttata
      781 cacttctttt tctagaaatg ctgaaccgcc aatcatattt tcacaggcaa tgatatctaa
      841 agatgttgtg cttccttcct tttctctttc ttggatacct tttgcaatca actcagcaat
      901 aaaaggtaag atattcgggc caatggcggt agtaactaaa tctgcttcag cgatttcttg
      961 gattactttt tctggatttg tttgattgtt gattcctttg acatttttta cagtgatttt
     1021 ttcttttgat tcatctgcta attcgatttc gtattctttt ctttcattta aagcatcaat
     1081 gatcgtgtga ttcacatcta cgaaagtaat ctcaaatcca ttagctgcca aaacctctcc
     1141 aataaatcct cgaccgatat ttcctgcacc aaaatgtact gctctcattt ttctctctcc
     1201 ttaagcaatt gctagatttt ccgttacttc ttctttgctg agcgcgtctg ctagctggac
     1261 aacattgtcc atatcactgc aataaagtgc gatttgctgt acaagttgca aatgctcatc
     1321 tccgactcct gcaataccga acaaaacagt cgcaatcttt tcttcttctt cggtaccaaa
     1381 atctacacca tctggtactt gaacgacaca gatgccggat tttttcacat atttttttgc
     1441 ttcatctgtt ccatgaggaa tcgcgataaa attccccata taaacagata acattttgtc
     1501 tctatctacc atggcatcga tatattcctc atcaacgcac cctgcttcta caagcatctg
     1561 accgcaatag cggatcgctt cttctttggt tttaaaagat cgattgagct cgatcatgtc
     1621 gaatggcaaa ctttgcatgc tgttttcctc ccttaagctg tctgtttcaa atattgtgca
     1681 atcgattcgt atttttgcgg attgaccatg ctgttgactg ataaatgaat ggattgaggt
     1741 gcagccatct gtactttttc cgctaaactt tctttggcta cgatcagtga atcaggttca
     1801 tcttttaaat cttggatatt gacttttctt aacgggatag ttaaatggtt cttgtctaat
     1861 atatctttga gcacggtcat ccccattgtt gctgatccgg aacgatcatc gtggacaagt
     1921 agaatctctt tgatttgttt gatgtttgct gaaagaaact cttctgctgg ctcattctca
     1981 atttcagaaa ctgatgtttt ctgcttgctg cttaattctt gaatgatttc atcatatcga
     2041 ggtgaattca aaaagttttc tactgagaca aaacgcgcgc tcggtgcttt ttgttttgct
     2101 ctttcttgta gttcgacctg agtaacgatc aatgtattcg agtcatctgt cagttggctg
     2161 attgcttgat tggtcaccgt ttgaggcaag tctgcttcct gtactttttt tctcaagatc
     2221 gaagcgccca tcgcacttga tcccattcct gcatcacaag caaaaatgat tttttgtacg
     2281 ccagagaatg tagctccttg aggaatacct tgggcacctt tggaagcttg cttagctgcc
     2341 tttgtctctt gaacagattt ttcgaagtct tcatttgtat ctttcgcatc cgctttcaaa
     2401 attactgcag aaataccaaa cgaaacagct gcggcaacaa tcacgcccaa aataacagga
     2461 aggtaagaat tgaacggtgt catcgctaga atcgcaatga tcgaccctgg agaagctggc
     2521 gctcttagtc cagcatccat caattggaaa atgaaacttc ctgacattcc tccgcctata
     2581 actgataaga ataataacgg tttcatcata acgtaaggaa aatagatttc atggatcccg
     2641 cctaaaaagt gaatgatcat ggctcctggt gctgaagatt ttgctgctcc tttgccaaac
     2701 aaagtaaaag caagtaatac gccaagtcct ggaccaggat ttgcttcaag caagaataga
     2761 atagattttc cagcctctgc tacttgttca gttcccaatg gtgtcaaaat cccatgattg
     2821 attgcattat tcaaaaataa gatttttgct ggttcaataa aaatacttgt caatggaatc
     2881 aaatgggcag ataaaatgat ctctacacct tttgccattg cttgagtcaa tgcatcgaca
     2941 actggaccaa tcgcagaaaa acctaataac gctaatccaa aaccgataag acctgctgaa
     3001 aaattattga ctagcatttc aaaaccggat ttgatccgtt tttggaatag ctgatcaaat
     3061 ttcttgatgg catatccacc aagaggtccc atgatcatcg cgcctagcat cattggaata
     3121 tctgttccga caatcacacc catcgttgca attgccccaa caactgatcc gcggtcacca
     3181 gcaattactt ttccgccagt ataaccgatc aataaaggaa tcagataagt tagcattggg
     3241 cttaccatgg tagccaaagc ctcatttggt aaataaccat ctgggataaa tagagccgtt
     3301 aatacacccc aggcaatcag tgctccaata tttggcatga ccatgcttga taaagtactg
     3361 cctaattttt gtaccctcgc ttttagcgaa atgttgtttt tgggaatcgt ccgttccatt
     3421 ttgtctctcc ctctctcctt ttgatactct aattgtatcc gcttactaaa aagagaaata
     3481 gtcgtacttt ggcacgaaac tttgtgccaa aggtttgagt atgtcagcaa aataaaaaga
     3541 tttcggtatc agaaaacttc aattttaaat aaaactgtta ttttaatttt tttataatat
     3601 gcaaacaata acttttgtta gcatttaatt tagatgtttt ccattctatt atgttattaa
     3661 attttatatt ggaggcctat ctatgacatt actgatcaaa aacatgaaca tgaaacgtgg
     3721 taaaaaacgt gtgattcgac aattgaatct tttagtagaa tcaggagaag tagctgcttt
     3781 gattgccccg aacggatttg gcaaaacaac tttgctgaat ggcattgccc aactgctgcc
     3841 aacaacttat gatcaattgt cccttcacaa catctctcct actgcttata cagattatcg
     3901 ccggtctttc ttttttctag aatcaacgaa tcagttatcc gactatctat cggctgttga
     3961 ttatttgact ttgatcaaat actattggca gagcaaagta gaatgccaaa ctgttttcca
     4021 acgattgaag atgactgagt accttacttt gccgattgga aaaatgtctc taggaatgaa
     4081 acaacatgtc ttgcttgctg cttacttgat cagtgattca tctgtcatgt tattcgatga
     4141 accgttgaac ggattagacc caggaagtat tgatatcttg aatagcctca tcagacaatg
     4201 gcagaaagag gggaagacga ttcttctttc ttcccacgct atcgcgaata tccaatcgat
     4261 ttgttctaaa gcatgttttc taaaaaatgg agaaatcgtc acagaatcct caaatatgga
     4321 agagatttta gctatttatc gtaaattata tgaggtgtaa ctatgtcttt acttacattt
     4381 gaacgcaatc tcttactaaa aaacaaagta aactttttat ttcctctatt gttggtcgtg
     4441 ctctttgcat tcccactttt ttttgatcat aaacttgctt atacagaatt cgatgaactg
     4501 aatcataact atgaagagat gcaacgtttg attgaaacgt tgaaagaaga tgagaatgag
     4561 aaagaatttg tggaaagttt agagaaaagc aacaaactga tcgaagcaat cttacatgca
     4621 aaaaacacag ggaatgtcca gcaaatggtc gaagcaactt atcattatga aaaagatata
     4681 ctggatcgac tgatttctgg tcaaagacaa ggaattccaa tcattgaaca acaaaaacgt
     4741 gtcgagttgc tccgttatat gaaagaacat cagatccaaa gatattctat ttttgacttg
     4801 ccagcgcatc tccctttagc aaattactac gaaaatattt tttcaggaat gatttctagt
     4861 tttttgattt tatgtataac cgccttgttt ctttcttcca tcatctctta tgaaaaaaga
     4921 aagcaggtaa tctctctagt caatctatta ccggatagta tggtaaagaa acatagcatc
     4981 cgttttacca tttattatgg tgcagctatg ctaagtctcg tgatgccatt tttgatcgtc
     5041 tctatactag taataatcaa aaatggtttg ggagacttcc gttatcctgt aggaacgatc
     5101 attggtcagg agatacgaat ccttccgatg tatgagtatc ttttccagag ctttctgttc
     5161 ctgttgttgt gggtgctctt tttaagtaca atcagttttc ttctatctgc tttatttgaa
     5221 cattctctag tcaatctttt aggtacactt ctttgtcttt tcttagcaga gtatcgcttg
     5281 ttttcttcga ttggatggat agaatcaatc agtcattact tgccaacttc ttatgttgat
     5341 tttcaaaatg tgatcatagg aggagatatt ttctcgccac tagcttcaga acaggttact
     5401 ttcatgaatg gtatcttgac acttggtata tggagtatcg ttttattatt cataggtatg
     5461 ggcactatct atataaaaaa gagctattaa aacacaaaaa taataggggc ggtgacaaaa
     5521 accttgtcac cgtcccctat ttcaaataaa cggttttcta gaataaatag ttttcaaaag
     5581 cttgcatccg cagtaataag aacctcccaa caaaaatagc tcctcttatt tttatcttgg
     5641 ttgaagttat tacttgatgc aaggcagctt tttttactgt gaacattggt tatatatgtc
     5701 catgttcaaa aatcatttgg acttcttcaa tggttttcgc atcactgagc gccattactt
     5761 ggtcgatgtc tgaacagaaa aaggctaaat cttggagcac cgctaactga tgttcttttt
     5821 ttaaagccac tacaaataag agtgtcgcga tttttggttc ctcttttgtt ccaaagtcca
     5881 caccatcagg cacttggact aaatagattc cttcttctac tacttcttca tctttgtcag
     5941 tcccatgtgg caaggctacg aaattcccga tatagacact cgctttttct tgtctttcga
     6001 tcattgattg gatatatgcc ggtttgattc ccccttgttg ggtcatccac tggccgatct
     6061 cggtcaacgc ttctttccaa ttttgatagg attgatcgag caatattttt tctgctggta
     6121 gctgtatcat cttttcacct tcatttcttt atttcttcaa tcaggatttt cgctaacaac
     6181 tgataaacga tttctttatt tcctgagcgg aagatctcgg tgttcaagtc attcatgatg
     6241 attgctccac tgatctttcc taaaagaatc ccgtcttttt cttggattgg cgaaggcgcc
     6301 agcataacta acatacggga caaatcgact ggctgtctat ccatcccttc aatggtcaac
     6361 ggatgtttca aattgaaaat acaaaagatc ggtttagaaa tcaatgagct ggatgtatgg
     6421 aataacccca tatttgtatg aggaataccg ataggtgctt gttcatatcg cttaagcagc
     6481 cggcgatgaa ccacctcttc atccgttact agtccttcag gaaaataagg aaataagctg
     6541 cgaatcgttt cggaaatcgt ctcaggatta tccagttctt ggataaaaaa acgaccgagt
     6601 aattgattga tttgatccat gaaatgcatt gtttcctcat aagtatcttc tatcatccac
     6661 tcgttattgc tgttatacgt aattttttgc ctttttttct tctcaatatg cgaaaaagct
     6721 tcttttagct gcttgacttc atcttctagc agtagcgggg agactaaaat atattttcca
     6781 ccgtatcctg gtaaaataga tgtagaaata acaacatcat attcttcttg taaatcgatt
     6841 ttttttaaat cagcaatccg atagaaatga atttgttgga tgaagggaaa ataccttttc
     6901 aatcgtagtt ccaacatgct agtagaagct aatccacttg gcgaaaatcc tgcaatactg
     6961 atattgcttt ctttcggact tcgttccaat gaattcgcaa aatgaaggac catataagcg
     7021 atctcttctt cggataattt tttttcttga aaaatccgct ctactgcttt acggattgca
     7081 ttcgcaactt cctcgtattg aaccatgatt ttttctaaaa tcggattggt cagtgtctct
     7141 tcttgtaaaa cggctcttga aaacacacca gataaatgag tcaacagcat tttgtacaaa
     7201 gtttcatctt cataaaaatg gacattcgtc tcttcactta cttcttgaat cagttgtttt
     7261 actaaataag caagctgtgt atcgaacgtc tcttcaaaga aattctgatc aaatgagttc
     7321 gtgaagtcat ttaataatcc tgcaaaaaag acgatctcat ttactggata aagttgtttt
     7381 gtctcagaag cgatccgtga aaacaatcgt ttagctgtct gtagtaattc tttgtttgcc
     7441 agactattat atgtctcatt tgtcagtaaa tgatttttag tcacacgctc catcgtcaat
     7501 gtcagcatca aaaccaaatg ttcaagcttt ctatcactta gattggtctt tttttggatc
     7561 aattcttccc cgattacttt gcgtgcaaga gatagatgtt gcggatcaac gaatcgaaaa
     7621 aaataattca gtgatgcatc gaaagaaata ctatgaaaaa gttcatattc attgatttct
     7681 aattctaata cattcgctgt cagaagccgg cggtattttt ccggtccttc gatctcgtat
     7741 ccctggttcc gaatgatttc caaaggcagt tggcgaatac tttcttccaa ctgtttgata
     7801 tctgcataaa aagtggtatt actgatgcga tatgattcca tgaaatgagc aagactcaac
     7861 ggctctgtcg tcagtaacaa ctcggctaaa atcgcgtgtt gtcgtttagc agtggataag
     7921 tcattacttt tcccatctgt attttctttt aattgctcta atacttcttc tgatgcctca
     7981 atgacaaatg agcctctagc tgcttttttc aaagaagcat ttaccgattt caatgagact
     8041 tcaagattat ctaattcgcg gtaaatagtc cgtttactga ccttcaataa attcatcatt
     8101 ttttggatag atacaggtga cgggctactg agaaattctg ctaataattg tttttctctg
     8161 ggcgataaat acatcttata tttctccctt cacgagtttt tcgtattcat tcgtagctac
     8221 ccaatgttca actagaacta gccgagggat cttttcttct agctgttcag ttacagctag
     8281 ttctctttgt gcgatataaa tcgtttcttc ttggattttg attttttcta ttgattcttt
     8341 tgagataaca aactgttttt tcgctttttt tgctaattga cttaaaatcg tcatgcccat
     8401 agtttgtgag ccgcgtctgt tgtttttata caaaaataca atattagcag gcgacttttt
     8461 ttgttctgtg atatctgcta tctctttttt ttcttcccgc tttggaaatt ggatcgcttc
     8521 ttttattcgt gacagcaaga tatctgtttc caaaaagtga tccagtacat agatttgact
     8581 ttgaggtact ttttctcttg caagacccgc tagttcatgt tgcacgataa ccagctgatt
     8641 tacttggtta ttcaattcat agatagagct gtatccgaca gaaagatcga ttttttctgc
     8701 tgctaatttc tttttcagca gggcggctcc catagcactt gatcccatac ctgagtcgca
     8761 agcaaaaatg acggaatgaa tgatagatgg ttccaagatt tctccccttt ctttgaccgt
     8821 tttgttctct ttttctttat ttggtaattt caatagtatc gctgcacaaa tgaaggtcac
     8881 aaatgtactg attgctatcc ctatacttac cccagctagt tgtggataag gcgcattggc
     8941 aaaaatcacg agtagtgacc caggcgatac aggtgcagct aatcccgtat ctaaccattg
     9001 aaatatcaac gtgccgcttg ccccaccagc cattacagct aaaaatagat acggtcgcag
     9061 caagacaaat ggaaaataga tttcgtgaat cccgccaatc gcatggatca tcaatgctcc
     9121 actcgcattt actttttcct gtttttctgc aaaacaaagg atagaaagta agatccctaa
     9181 accaggtcca ggatttgcct ccatcaaaaa taaggaagag tacccgactg tttgtgagaa
     9241 ttcgattcct aaaggtgtca atacaccatg attgattgca ttattgaaaa atagtacttt
     9301 tagaggttct aagatcagac tgacaagtgg taacagattc attgtgacta gccaagacac
     9361 aacggaggcg agcaacaagt gaacactatt taaaactggt tcaataaaaa cgattccaat
     9421 tatacaaata acgctaccag tcagtcctac tagtaggttg ctgacaagca tttcatatcc
     9481 agccttgaat ttcacgtgga atctagaaac aaattgttca tatacgatgc cggcaagcgg
     9541 cccgataatc atcgctccta cgatttgtgg agcattggaa cgagcgatca accctaaaga
     9601 agcaattgca ccggcgactg cccctttttg tccccctacc gatttcccgc ctgaataact
     9661 gatcaagatg ggtaaaagca gctggatcat ccaatggcat acctcttcta atgtggtgtg
     9721 caaatcgcca gacacacctt ttgttgctaa tgacaagatt ccccaagcga taaacagact
     9781 tagatttggc atgatcattt catttaagtg atgtccagct tttctcagtt gtttcataga
     9841 cacgcgactg ctgttgtttg ctttccattt gtattccatc tttttcacca tacttctatt
     9901 tattgatgga cggattttat gtagaaaaag cagcaaacga aaaaccgtct gctgctttca
     9961 gctatctggg aactatttgc ctggttcagt cgcaatgatt gctagttcac cttggaccgc
    10021 ccactcagaa acgccatttg gaataataca gcttgttcct ttttctaatg gatatgtttt
    10081 tccgtcaacg acaaggtctc ctgctccttc gattaccgtc atcaatgtat aaggggcttg
    10141 ttttttgaaa gatgtgattc ctttaatatc ccactcatat acattgaaaa actcagtttc
    10201 taaataagtg acgatcgatg aattcccttt tcggacttct ttgatttgta attctggtgt
    10261 tttcgcaggg acagttgtta catcgatcga ttgttggata tgcagctctc ttgtttgtcc
    10321 ttgcgcatct tttctgtcgt agtcatacac gcggtaagtc gtgtcagaac tttgctgtgt
    10381 ttctaaaatc atgattcctt taccaatagc atggatcgtt ccgcttggta cataaaagaa
    10441 atcacctttt ttaactggta ctttcttcaa aaggtcatcc cagcgcccat ctttgatcat
    10501 ctcagctagt tcttctcttg tctttgcatg atgaccatag atgatttctg caccaggttc
    10561 agcatcgatg atataccagc attcagtttt tcctaattca ccttcatgtt tcatcccata
    10621 ggcatcatct ggatgtactt gtacagatag atcatcttct gcgtctaaga tcttgatcaa
    10681 taatggaaac actggttctg aaggatggcc aaacaattcc ttgtgttctg accatagttc
    10741 atctaatctt ttccctttga attcgccatt ttctactaca ctgacaccat gaggatgcgc
    10801 actgatcgcc caatcttctc cgattttatc gttagggata tcaaaaccaa agactgaacg
    10861 taaacgactt cctccccaaa ttttttcttg aaaaactggt tttaaaaaca taggttcctg
    10921 cataattcaa actcctctca tttttcatag ctattatagc attatttcgg taataccgct
    10981 ttctctttca aaaagttttc aacaatttca tctcgacata tgagatactc cgcacgatga
    11041 tcgacaggat gtacgcgatt tgacaaaaat atcaacgctt cttgtgtttt tatatcaatc
    11101 aagaaaaacg tacctgtata accagtatga aaaagaatcg catgaccgtc ttctacatcc
    11161 ttttttaagt cccatcctaa agaacgatag ccattttttt tcggtgtttg atcttgcagt
    11221 aaagaatcaa tcgtctcttc ttctaaaaaa cgaccattac ggatttttcc cttgttcaaa
    11281 tacatttgga caaaagtaat gctgtctttc aaatcaataa agagacccgc gttacctgca
    11341 tgttcagcca aaactcttgc tttcggatca tgagtttgtc cacgcaaaac ctcacccgaa
    11401 ggcagttgtt ctgtgggaac gatttttttg gaaggaaaag gaagaaataa gctgttcatc
    11461 attcctaatg gttccaatac ttgttcttga aagatttcca ctgcagtttc ttggtaaatt
    11521 tcctctagca tcaacccaag aagaatcgta ccggcatctg tatattttac ttcttttcct
    11581 agttgctcac ctggttctag cttcaaataa gcggcgatca attcttcctg agatagctta
    11641 tctctgtttg gtatccatgt tttgatatca gaagtatgtg tcagtaaatg acgcaaagtg
    11701 attgtctgat tttcaaattc aggaagatat ttatgaagcg gttgatcgag ttctatcttt
    11761 ccatcttcta atagacgtaa aactaatgaa gtggttgcaa cgactttcgt cagtgaggct
    11821 gcatcaaaca acagatcttc tgtcatcagc tgcttttcag gaacgacttg ggcaaagccg
    11881 gctgtaaatg tttctacttg attttctttt atgaaggcga ctgctacacc tggataaaat
    11941 ccttcttcca tctttttcaa aataagctgt ttcgtgtgtg gatacatctt tttctcctgc
    12001 ttttctatca gattcttaca gtccaagctt aaagcttaat ctatgaggaa tcaacctttt
    12061 ttacacaaaa aaactgcgcg aaaaaccgcg cagtttcccc cggtatacta ttttctcttt
    12121 ctatctctag aataagcgat agtatgaccg ttcataaaat gacgagacat gcgacaatcg
    12181 ttaagaaaga aaaagttaag cgtcgcatcg aatgtcagtt ttattctttt cttttggctc
    12241 actgacgaac caccattcga ttcctatagc atcatagatt gtgagtactg tgtgcttttt
    12301 gtctaagaag aaatcaactg atagttgctg caagtgagca atcagtcctt ccatgctttc
    12361 agtatcgatc atgaatttca gaaaatcaag tccaatcact tcatctgtag gcaaatcaat
    12421 tgcgcctccg ctagcttctg tcaaaccaac atggaaatct ccttcattca atacatggat
    12481 gccctcttct tcattatgta ccttcaaacc aagtacatga ttaagaaatt ggcgttcttt
    12541 tgttaagtta gtaacattta aatggatttt ttcaaaataa gaacctgttg atagtttact
    12601 gaatttttct tcagattttc ttaataatgc ttcttgatcc attgtttctg gaatttctct
    12661 ttggtcgttc ttattctctt catggtaaat ttccaattca ttctcttctg ggtctacgag
    12721 caaaatcccc atacgtccat gatcttctaa agcttctttg attggataat tcattttacg
    12781 gatacgacaa aggatatctg ccatttcttc tgtgctaggg ataatcaatg aaaaacggtt
    12841 cagctttttg atcaccccca tgtgatcatt cgctctagga ctttcttcca gcagcaacat
    12901 ttcacttcca gcttcttctg ttcctaaaat ggctaattcg ttttcttctc ttttcaaatc
    12961 aaagccaaca acgttcttgt aaaaatcaat cattttatct cggtctttca cccgaatggc
    13021 tacacttttt aaataggtgg cagcccccaa ttgaaaatct ttcatatttc ttgctcccct
    13081 ctacgtcaag ctttatttta acgatggatt cttcttcttt caagcaataa ctttgatttt
    13141 gttaaaaaaa atgtgagaaa attcgcattt tgcttgtttt ttgtaaatta atgagtaaaa
    13201 actcttataa aaagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
    13261 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
    13321 gaacaccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca
    13381 aaacggaaaa atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg
    13441 attgaacacc gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa
    13501 aaaaataata aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa
    13561 agatctgcat agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc
    13621 gaaccatgat tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca
    13681 acaacgaaac ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct
    13741 gttcgtgtat ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag
    13801 aatgcccgcg caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg
    13861 tttaatacag tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct
    13921 ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca
    13981 tagatacttc ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat
    14041 tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca
    14101 tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca
    14161 atagcgacca tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct
    14221 ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct
    14281 ggaaggccag atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg
    14341 gctgaagcaa ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga
    14401 gagaactgtg ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt
    14461 gcaactttta ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc
    14521 atcaacggca tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc
    14581 tgattttctt tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg
    14641 gtatgaccac catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga
    14701 tctgcgaaaa acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg
    14761 atcatcaata acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc
    14821 ccaatcaatg tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg
    14881 atgttatcca tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa
    14941 taggaaatcg gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt
    15001 cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa
    15061 tacatcacga ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt
    15121 gttacttgtt ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct
    15181 gtggttgctt tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct
    15241 agtacaaata cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt
    15301 accgttcccc agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca
    15361 ccatgtcctg gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta
    15421 agtcctcctc tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata
    15481 gttcttcatg tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg
    15541 catccattac tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg
    15601 ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat
    15661 caaacacata gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt
    15721 gtccaccaga gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct
    15781 ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg
    15841 catccggctt accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt
    15901 gaggaacaaa gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc
    15961 catttatttg gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg
    16021 tcgttttacc tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga
    16081 aatcaatatc ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga
    16141 aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga
    16201 taccgatttt ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca
    16261 ccatgataaa aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca
    16321 taaatgcaat cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc
    16381 aagtgattgc tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga
    16441 cgatcgtatt aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat
    16501 tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc
    16561 gaaataccag gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa
    16621 agtacatgat tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg
    16681 tcaactgatg atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat
    16741 tgatcatcat acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg
    16801 ttcttgtgat caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg
    16861 attttttata gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa
    16921 aaaatgtatt tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac
    16981 ctgtatgcca aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg
    17041 taagtgtcgg caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa
    17101 ttgctgaagt caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt
    17161 tatatactcc gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta
    17221 ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag
    17281 tattcatcac taattggatc agtaattcca attcatgatc attttttact ttcagcttcg
    17341 tccgatcgat caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt
    17401 gaggcgtttt ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt
    17461 tcataaacaa gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt
    17521 tagaaaaata ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta
    17581 acatatcttt agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt
    17641 tatcttcaaa atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag
    17701 caatcgaagc ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc
    17761 ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg atcctccttt
    17821 ctttcaagat gacacattgt catatgacgc tgtgtcatta taacacctcc tacctgtttt
    17881 gcaagacatt tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg
    17941 aatccttcca tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg
    18001 atgagataaa tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct
    18061 gacagcatcg ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac
    18121 tcgctttcta tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa
    18181 ctttaatata ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact
    18241 gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt
    18301 gcttgaatat tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc
    18361 ttttataccc tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag
    18421 ccttttcttt ttctcatagt tataacaatc atagcaataa aaggagaagg taagcatatg
    18481 gcatctgttt tagtagtcaa aggtcatccc ctaactgcag aagaatcgcg cacagttaag
    18541 gcattgacgt cttttttaac aagttataaa gaaaatcatc cagatgatga agtgactgtt
    18601 ttggaacttt atcgcgatga tattccggaa atcgatgaag aattattatc aggttgggaa
    18661 gcattacgtg ctggtgcaga attcacttcc ttatcagaaa gtcaacaaca aaaaattgct
    18721 cgtttcaatg aattgactga tcagttcctt gctgctgata aaatcgttat cgcaaacgcc
    18781 ttatggaatc taaatatccc aacaaaatta aaagcttggt tcgataccgt caatgtagct
    18841 ggaaaaacgt tccgctatac agaaaacggg ccagaaggat tagtgacagg taaaaaagca
    18901 ctgcacatcc aatctaatgg cggtgtatac aacggacaag actttgcctc tcaatatgta
    18961 aaaggtattt taaactttgt aggcatcgat caagtagatc agctattcat cgaaggaatc
    19021 gattatgacc ctgaccgtgc agatgagttg atgcaaaatg cattagataa agctgctgct
    19081 ttaggtaaat cattttaaaa attgagataa atgcatacaa aagagacaga ggaaaaaatc
    19141 ttcaaagcag catttgtacc ttggatattt tcccatctgt ctctttttgt tattctttct
    19201 gctttttcaa cggattttct tcataaagag aagagattgt tgtgtaccat tcaaaaacat
    19261 gagtaatcac tactttagct gctgcataaa caggaatccc caagatcaca ccgatgattc
    19321 cgaacaattt cccagaagtc agcagaacta ataaaatcgt tactggatgg attgctaact
    19381 gattccctag tactaatgga gagatcaagc gtccttctat tgtctgctct actgcaaaaa
    19441 cgatcagtac tttgatgatc atgaatggac ctgctacaat ggctaaaaag atagccggaa
    19501 tcatcgctaa aaatgatcct aagtaaggga tcaggttcaa aaaacccgca ataatcccca
    19561 acgtgacagc atactctaaa ccaatgaccg caaaaccaat cataaacatg attgccacag
    19621 caaaagccac agttagttgt cctcttatat aagaagaaac ttgactattc atttcactca
    19681 gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta
    19741 attgtttacc gtctttcaat agataaaata agataaacgg catcgtcagc agagccacta
    19801 aaatagtcgc taccgcgccg acaaaacttc caagactctg aactgtactc ttagatatat
    19861 cttggatcat atcgcctgcc gaattcatga acttatcacc tgctgtttcc aactgttcgc
    19921 gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa tttattatca atcgtatcta
    19981 catattgagg gaagttactg atgaagctca ctgtttgttc ctgaatctta gggatgatca
    20041 ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa
    20101 gccttggtac ttttcgcttt tcaaagtaat caactaccgg attcatcaag taatacaaga
    20161 ttcctgctaa aatgataggc agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca
    20221 aatatgaaac ttttgtaaat aaaaagacga tcagcaatat taacaataca acaagtaagg
    20281 ctgtgactac ttgattattt aaaaaccagc gccaaaacca cgataccggt ctgttcttct
    20341 tattttttcc ttccataatt cctcctgtta gttataaaaa atttggcttc ccaaaccaat
    20401 agcaggcagt ctatgcggat gaagatagat tccatttgca atttgtcctt cttctggaca
    20461 agcatcaaaa atcaatgata cgtgagctac tgtctctaaa ttcccattgg ccatcggacc
    20521 gacataatcg atcgtatata cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga
    20581 aaaaggttcg tcttttgata cttcttgaat caccgaatat tttcgtaagg cagctgttgc
    20641 tgttttatcg aacaaaatca gcatgggttc ttccttgttt attgcttctg tcccaatttc
    20701 tgtaatcttt gcctgaatca ttgcactctc tcctttaatt gctttcaaaa acagtataac
    20761 acattctcat tctttagccg tgtggaataa ttatgtgata gaataaaaac gaaaactatt
    20821 tcattacaat taggaggaat cagctcatat gatcgaaaag ataattttag gaacctatac
    20881 gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat acagaaaaag aagaattagc
    20941 caacctttca ttagctgttg aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg
    21001 caatctttac acagttacta gtgtggatgg attaggtggt gccggtgcgt acgataagga
    21061 ttttcatttt ttgaatgcgg taacagagtc tggcgctcct ttgtgttatg tagcggttga
    21121 tgaagcacgt caattactat acggggcgaa ctatcataaa ggtgaagtaa atgtctacca
    21181 tatattagga gatggcgaac tgaaagcagc agattcaata ttccatcaag aagcgacagg
    21241 tcctcataaa aaccaagatc atgcgcatgt ccactatacg gatctgactc ctgaccaacg
    21301 attagtcgta tgtgatcttg gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg
    21361 caaattgaat cttgtaacta ccttcactgc agagccagga acaggccctc gccatcttgt
    21421 ctttcatcca aatggacaat tcgcttattt atttggtgaa ttagacagca cagtccgcgt
    21481 gctttcttat aataaagaaa caggtgcttt tgaagaatta cagaaaatat cgaccttgcc
    21541 agaagaattc gacggtgaaa atggaggggc agctatccgc atttcaaatg atggcaagtt
    21601 cctttatgct tctaaccgag gacataactc gattgctgta ttcgcagttt ttgaagatgg
    21661 ttcccacatt caaaacaagc aaatcatctc aacagaaggg gattttcccc gcgatttcgc
    21721 actagatcca agcaataatt ttgtcgtgtg tgccaatcag aacaccgata atctgacatt
    21781 atatcgccgt catcctgaaa caggattact ggaattactg aaaaaagatg tctatgtacc
    21841 agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca
    21901 agacgtatta gacagactac gggaagaact aaatattcct ttctttgatg ggaaaatcga
    21961 agataaagag tatagcgaag aggaatatca gaaactcaaa gccgatttac agaactactt
    22021 tgaacaatat gtacagaatg tagaaaacta atcagctttc catactatct gaaaagaaca
    22081 tcaggacaag acttttccgt ctatcctgat gttcttttct acaaataaaa aaccgccgag
    22141 atggttagac tcgacggaaa aggagtattt actcgatttt gagtaagtac aaggttgttg
    22201 ttggttatag gtatgtcatt atcataaagg attttgatca ctctatcata aaggtaatat
    22261 tacatttata ttagataact ctagttttta ttgttatcaa agatttcttt tattttttca
    22321 acaacaaatt agtcattttt tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt
    22381 ttgcttcaga tgtattgaat agatcttcat ctacccctga ttcaagatcg atactttgga
    22441 tcatttcttc acagcctgct acatatgaaa gataagcttt ttcaaaattt ttatgaatgc
    22501 ccattgcttt tgctggtgga cgcaaagtac tgactttctt aagcattgcc cgatattttt
    22561 ttgttccttc ttcaaataac aggatgatct cttcaagagt cgatttgtcc agttcacttg
    22621 tttttccttc atcaattgcg tttctgatca cttcatattt cggatgcatt ccttctccga
    22681 ttgcttctgt ttccttgacg atttgatcga tggactgcac ataatagcca atattcggta
    22741 acatgttcgt tttcctactt tctccattgt tgttattatt tattctactt gtttttatcc
    22801 tcaaatgaca agtacttgaa aaaatttaat ggtttctata cagctcgcca ttcttattcg
    22861 tgctcaaaga aaccatctgc taaactatat tgtttccctg cgacaatgtc atattggatc
    22921 aattgatatt cgtcgtaaat ctcttgttgc gagcgattga attgcgcttc tttataccac
    22981 tgtccgtttt gatagtacag ttctcgttca aacgccggaa gttcctgttc taatgataaa
    23041 agcagctgat aaaagccatt tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa
    23101 taaaacggac tggtgattgc atcgtgagtc gtttgctttt ctaattctcc tttactgtcg
    23161 aacataagaa attgtgtttc atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct
    23221 gaatagatcc ctggaagatg atccccccaa aaaacaacta aagtccttct agaaagattc
    23281 ttcaattctt ccgtgaactg cttcagtgcc gtgctgctat aggaaatatc ttgcagataa
    23341 ttctctaagt caagtgtccc acttccatct gaagtttttg caggatagtc tagattatca
    23401 tactttccat cgtagggcat atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt
    23461 ttttcttcct tgagaagtgt cattacttct ttgtaagctg actcatcaga aatatatgga
    23521 ttattctcta tcgtatccgt ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct
    23581 aatgtctgat aaacatcctc tcttttatac atcgaagtat tgtatggatg gatcgctgtc
    23641 gtctggtatg attgtgcgtt taacgctgaa acgatagaag gaagctgatc catttttggt
    23701 acaagcatcg tgtaaggggt ggtgagttgc gcattaaata acgccataga aaaaccagtc
    23761 aatgcttcaa attcgatatt tgctgttcca ccgccatagt tttgagaaag catattgcca
    23821 ctatatgttt gatcagctac ttcataataa tctgccaatg gctcaccaga tacttctacg
    23881 ccgtttaatc gactaggatc tgagaaactt tcactcatca cgaacacgat gttcggcgac
    23941 tcttcctcta cagttttatt tttttcatcg gctagcttct ggtatttttc agtgatttct
    24001 ttgatctttt ctttcgaata tccttcaggt tcatccattg gttctacctt cagattgtac
    24061 aaaaacccac cgataaaacc agtgttatag tagttcattt tctggctgta aggtatccat
    24121 agtgccgttt tattatatgc ttttcgcaac aaattatcgg gattgttgaa attactgata
    24181 taccctagca gtccaattgc cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt
    24241 ccttttttca ggcttcgaaa catgtaccag caaaacaatc ctatcacggt tcctgcggca
    24301 gctaagatca ctgtaaacag catagtgccg gtcatatctt tcagcaatcc gatttcagtg
    24361 atcatcttca aatcgtctgg ataaatcggt tcttgccgat aactcatttt catataattt
    24421 gcaaaaccta atacaccgat ggctacgaca tacgtaaggg aacctaaagg gacagatcct
    24481 gctagtgcaa tcagaaaaat aagaataatc agcaagacga gacaggctaa gaagaatttt
    24541 tccgtatgcc aagaaaaagc aaatttcaac gcaaggtcta cggatagatc attttgacac
    24601 cactgcaaaa ataaattact aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt
    24661 ccttttaaaa tctttttctg tttggtcgaa agtctgctta tatatctttt caaatgcttg
    24721 ctccttcatt tctatcaata aaagaatgtt gcttctctta ttttagttgt acgaaattcg
    24781 aaagtaaaga acggaaagaa gaattaatga aatccaaata ttctctttct ttatggcctt
    24841 tctggtttct ttaataagcg gacctttcgt actccagaaa ttttttctag ctgttgtttc
    24901 acttgattga gctgttcaaa atcagtactg tctatgtcaa tcaatatgta gacatgctca
    24961 ttcttatggt tacgcgatat atctgcgatc gctagctcgt tctcattcag cagagaaaat
    25021 atctctgcta atatattggt tttcttttga taaaatatag tgaatcgaaa cggcgcttgg
    25081 aaaaacattc gtgcggatgg ataattgacc gcttctctaa ccgtaccgaa caaaagataa
    25141 tcttttaagg cttcaactgc caaacgtccg ctgtctgcca aagcttcctg agtcgatccc
    25201 cctagatgag gcaacataag tatacggtca ttttctaaga actcttcttc tggaaaatca
    25261 gtcaagtatt tagccagatg ttgtttttcc aatgcttgca taacagcata tttatcaaca
    25321 atttcacttc ttcctacatt gataagtaca gcatcttttt tcattttttc tatattcgcc
    25381 tgatcgatca atccttttgt atcttctgtc aacggcagca tgactacgat gtaatccgag
    25441 gtagaaagaa ctgtttctaa atctgcttgc cgaacataat ccaactgggc atcgcggata
    25501 gaataaccca agacatccat ccctaagcta tagcaactta gcgctacttt tgtgcctatc
    25561 gctccaagac caagaagacc gatggttttg ccttgcaatt ctcgtcccac ataagcactt
    25621 cttttgtttt ctgcctgttc taaaatatta ggacctgtta gtgtttgtac catacgactt
    25681 gcttcgatta tcggacggga agataagagc aaacagcaca aaacaagctc ttttactgca
    25741 ttagcattta cacctggtgt attcatgacg atcgtcccgt tttcagaagc tttttcaacg
    25801 ttgatcgtgt tcaccccaac accagctctt gaaattgcta gcaaatctgg tgtaagccat
    25861 tcatctttca ctttcgtact acgaataact attccttcgg ctggcaaatc atctattatt
    25921 ttgaaatatt ccccttgtaa cttttctatc tcttgttctg gaatagcatc aatcaaatga
    25981 acgtgataca taaaaccccc tccttactaa caagtatctc gaaatttctg caattagaca
    26041 actgtttacc atttgacttc ctagaagcaa aaagagcagt tagagcagag taggctgagt
    26101 gataaaaata aggctatgac aagttactgc cataagcctt attttcgaga gcaaaatatt
    26161 gagggtgaga tatcttgata aatggtgttc cgttagctga cattgataat aagactaaat
    26221 ttcaaaaaag ttgaaaagca aatttcgtca atctcttctt atttcttaaa actaagtggc
    26281 tttttcacaa cctcttttta tctaataacc actgtttcca actcaactaa ttttgcccac
    26341 gtttcaattt gatctgttgt tagatttagc gagacaacgg tatgatgacc gccacctgcc
    26401 tgaatccatg aatggacacc ttcatggaaa ttcggtttag ttttccataa cacacgtgca
    26461 acaggtaaat taggtgcttc agtcgttggt tcaaaggctt ctacttcatt gatcagcagt
    26521 ttataatgcg taccgaaatc tgccatcgat acgactaccc cttctcctgc tttcccatca
    26581 aatacaagac gcgccggatc ttctctatct cccattgata aaggagaaac aacgattctt
    26641 ggtttcgtcg cagcaagcgt cggatctact tccatcatat gggattccaa gattgcttct
    26701 tggccactag ctagctcgta agtataatct tccatgaatc ctgtatcttt ccctcgagcc
    26761 atgattttca gcaaccgatc aatcgctgca gttttccagt cgccttcccc agcaaaaccg
    26821 tatccttctg ccatcaaacg ttgtacagct agtccaggta gttgtttcat tccatacagg
    26881 tcttcaaaat tagtcgtaaa agcgttatag cctccttctt ctaagaaacg gcgaataccg
    26941 atttcctgct gagcttgtac ttttacatgt tcctcccatg tcttttcttc ataatcacca
    27001 tagtcgaagg tatacaattc cttgtactct tcaaataatt gatcgacttc ttcgtctgat
    27061 acgcgatcga ttacttgtac aagatctccg ataccaaaat aatcaacagt ccaaccaaat
    27121 tgaatctgtg cttccacttt gtctccttct gtgacaccca cgtttctcat gttgtcacca
    27181 aaacgagcca cttttattcc aaaactttca ttataggcta ctgcaacatc catccaatca
    27241 gcaatctctt tttggatctc aggtcgtttc cagtagccaa cgacgatttt attttgttta
    27301 tttaatcgag cattgatgaa cccatattcg cggtcaccat gagcggattg atttaaattc
    27361 atgaaatcca tatcgatcgt tttccaagga atgctttcgt tatattgcgt agctaaatga
    27421 agcaatggtt tttgtagaag ctttgtcccg cggatccaca tttttgctgg tgagaaagta
    27481 tgcatccaag tgatcacacc tgctacttct tctcgataat tgacctcttt catgattttt
    27541 gtgatcgtat cagcagtaac tgctaattct tgaaggcgga ttggataagg caattggcca
    27601 ttttcattca gttcatcgac catttcttgt gcatgtttct ttacttctcg cagtgcttct
    27661 tccccatata aatgctgtga tcctacaaca aaccaaaatt ctttttctcc aatattcaac
    27721 ataattagcc actcctttta tttgttttgt ccgtaatacg cattttttcc gtgttttcgt
    27781 aaataatgtt tatctaaaat ccgttgaggc agctcttctg aaaatgaatt gagctgtctg
    27841 gtaaataaat tcatcttgca tacttcatct agtacgactg cgttcattac cgcactttgc
    27901 gcatctttcc cccaagtaaa tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag
    27961 ggatcaattt tgcgttcttt gaatgtttct atgataactt ttcctgtttc atattcgtac
    28021 ccgctgtcga tttcctgttg tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta
    28081 tctgcatgcg tcgtccccat cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc
    28141 catgtcgagt gagtatggac gattcctccg atttgaggaa atgaacgata taaaacagca
    28201 tgagtaggcg tatccgaaga gggattgagt ttaccttcaa tgatatgatt gtccaagtcc
    28261 acaacgacca tgtcgtctgc tgtcatctct tcataactga ctccgctagg tttgataacg
    28321 aaatatcgtg tttcaggatc aaaagcgctc acatttcccc atgtatattt gacgagtccg
    28381 tgtttaggca agtcaagatt tgcttggtat acttcttctt tcagttgttc cagcattttc
    28441 tatcactcct ttttcttgca attgatgaat cgctgttttt tcaatgtcga tcccctcacg
    28501 gtaacgtctc ataaatatct gatagcctgc tatttcttct gctgaaggtg tgatggtgat
    28561 tccttggtct tgtccaaaca cgtcttctgc caagaagttt tctaaagact tcccatgtcg
    28621 atccatcata taggctgcta ataacgcgat tccccacgct ccaccttctc cggctgtgtc
    28681 catcactgta actggtgcct ccatagcaga tgctagaatc cgctgtccaa cttctggtgt
    28741 tttaaaaatc cccccatgcc ccactaaacg atcaatttgg acattctcag atttcaatat
    28801 atccattccg attctcattg caccaaaagc actggctaaa tggatccgca tgaaattcgc
    28861 taaatcaaaa cggctacctg gtgtacgtac aaataatggc cttccttcat ttactcctgt
    28921 aatgttttca ccggaaaaat aaccataaga aagtaatccc ccgcaatccg tatcgccttc
    28981 tagcgctttg ttgaataata ccgtgaataa tttatctctg cttatctcca tacccaaaga
    29041 attggtaaat tcttcaaaaa tactgaccca agcattgata tccgaagagc agttattcgt
    29101 atgaaccatg cctactagtt ctcctgaagg tgtagtgacc atgtcgattt ccggatggac
    29161 attttctagt tctttttcta atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt
    29221 ccctgtccgt ttggctacac tgttcgtagc gaccataccg gttcctgcat ccccttctgg
    29281 cggacaaacc gggattcctg gtctcaaatt tcccgtaggg tcgatcagat aagcccctga
    29341 ttcgcttaac ttgccagctt cttctcctgc tactagaact ttaggcaaaa tgtgatccaa
    29401 gtcaatcgct acgtgcgctt tttcgattaa ctgaccaaaa atatttttca tttcttgatg
    29461 gtaggttttc gttgaaggat cgataggaaa catgcctgat gcatctccta cgcctagtac
    29521 tttctcacca gtaagctgcc aatggacata acctgcaagc gttgtcaaaa aattaatttc
    29581 tttcacatgt tcttctttat tcaaaataga ttgatacaaa tgagcgatac tccatctctg
    29641 cggaatattg tattgaaatt tttccgttaa tattcgttct gcttcttcag tgattgtatt
    29701 tcgccaagta cgaaatggta ctagtaactg ttccttgcta tcaaatgcta gataaccgtg
    29761 catcattgca ctgaacccaa tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa
    29821 tacttctgct gccatttttc ggtaactgac gcgcagtcct ttccaaatat cttctaatga
    29881 ataggtccag ataccatttt ctagtttgtt ttcccactca taactaccag cggcaatagg
    29941 agtaaaagat gaatcaatca atacagcttt gatcctagtt gatccaaact caatccctaa
    30001 agatgtacga ccatctatga catctaacat actttgcgca tttacttcct tcataatgat
    30061 ttaactccct ccttctcgtc aaacctgatc aaaccgtctt tttagtagcg cttacaatga
    30121 ttattgtata cttttgtacg tacatgtcaa gcggttattt acaagaatac aataaaaaac
    30181 acttgttttg tacgtacata agttataaaa tgaaattgct tacaaaatct gatcaaccgc
    30241 cttctcgaaa ttctagaagg agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc
    30301 gtttatttat ttttttggcg catttggcgg cattctattt ggatatgata ttggtgttat
    30361 gaccggcgcc ttaccttttt tacagcatga ttggggtttg gcaggtaaag cttctttgat
    30421 aggatggatc acgtcttcgg tcatgttagg tgcgatcctt ggcggctctt tatctggtct
    30481 tctttctgat aagcttggac gacgaaaaat gattcttctt tctgcactta tttttatggc
    30541 tggttctgtc ctttctgcat ctgcccctca caatggttct tattttctga ttgcagcaag
    30601 gattcttcta ggtttagctg ttggcgctgc atctgcccta gttcctgctt atatgtctga
    30661 aatggctcct gctagactaa ggggaagatt gtcagggatc aatcaagtga tgattgcttc
    30721 tggcatgctc ctttcctatg ttgcagatta tttgctaaaa gtgttgcctg aaacgatggc
    30781 ttggcgcgtg atgttagggc tcgctgctgt ccccgcgctg attttatttt tcggtgtttt
    30841 agctctacca gagtcaccgc gttttcttat gcaatctggc cgcttggaag aggcaaaaag
    30901 agtattgaac tatattcgga caccaaaaga agctgaacag gaattcgaac aaatccagct
    30961 gaatgtcaaa caagaaaaga caaccggaac ttcttggcat actttgtttt tagaaaaata
    31021 tcgttcctta gtcattgccg gtattggtgt agcagtattc cagcaatttc aaggagctaa
    31081 tgctattttt tactatattc cgctgatcgt agaaaaagcg actgggcatg cagcgagcga
    31141 tgcattgatg tggccgatca tccaaggaat cattttagtt gctggttctt tgctcttttt
    31201 ggttattgca gacaagttca atcggcgaac tttattgaaa ataggcggta gcgtgatggg
    31261 actttcattc atcctaccag ctgttttagg aactgtactg gatgctcata caaattcgtt
    31321 gttgattctc ttgtttttat gtatttatgt cgcattttat tcttgcacat gggctccact
    31381 gacatgggta atcataggcg aaatattccc actagctgtc cgcgggcgag catctggcct
    31441 agcctcttct tttaattgga ttggttcttt cctagttggc ttgctttttc ctgtgatgac
    31501 tgcttctatg tctcaagaaa tcgttttcgg catttttggg atcatctgct ttttaggggt
    31561 tttatttatt caagagatcg taccagaaac tcgtgggaaa tctttagagg aaatcgaaca
    31621 aagtgcatct aaaaaaactt atcctaaacg tatttctatc catcattctt aaagaggttg
    31681 tgacagaagt ggtcagcttc aagaaataag aaggaaccgc ttagtttcca gtcacaagga
    31741 acaatttcaa aaaacagctt ctcatgtttg atgaatattg tgacttgtga ccagttggta
    31801 ggagaacacc gtttagtgga gaggtggtga aagaaacgtt cagccttgag cgatgacaaa
    31861 agctctcaca aacattgcga cgagtaaccg taggagcaat aagaggcggt aaaaacccgt
    31921 ctcttatttt tcgttttctt tcactgtttc ccgttcaact agtacaggtt catagagaat
    31981 atttggctgt gcctgtcctt tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc
    32041 caacttttct tttggatgcg tcaatgttgt caatttttta ttccctaaat ggcttaaaga
    32101 agaatcgtct gttcctacga tcgataggtc atctggaata tgatagccat tttctgtcaa
    32161 cttatctagc attaaactag ctatttgatc gttatagcaa atgatgccat ctaaagcttg
    32221 ttcttctttc aaccgttgta ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa
    32281 agtaatgata tcttccgtca aaaaacgcac attatactgt tcacacgcgc tgataaaacc
    32341 tttcatcctg tattttcctt gaagatcgtc gatttttgtg atgaacaaca gacgctgatg
    32401 tccttttttt atcaaatatt ctgctgccat aaaaccgact ttcacatcat ctaaacaaat
    32461 ataagaggca tttaactctt cataagcagc attgatcatc accatcggaa tgccttgttc
    32521 tctcaaagca acataaagtg ctaagtttgg gttatactga ttacttttcg taggctcaac
    32581 gatcaaacca tctaccccat aagcaatcat tttcttcaaa cactctttct ccaattcgtg
    32641 atcgttattc gtactggcta aaagcaatga atacccagat tctcgtaatt cattttcgat
    32701 tcctcggata atcgacggaa aaatataatc agagagatat gtggtgatga caccgatcgt
    32761 ttttgttctg ttcaatggtg ttttttcttt cttatagctg tcatctacat atgtgcccga
    32821 ccctttttct ttacgcagat agccctcatt taccaacaca gcgattgctt gccgaatcgt
    32881 atgtctgctg acgtcatata tttcttgcag cttgaattct gagggaatgg ttgtatttac
    32941 ttcgtattcc tcacgaagga tcttttgttt tatctcttct gcaatatctt gatattttgc
    33001 catttgcctt gccccttttt atttgtacgt acatatatca taacgagtct gcaaggttta
    33061 ttcaagcaaa aatcaaggtt tcttgtgctc ataaggcata cggaacaggt tcaccaaatt
    33121 ctggaaatcc ttgctcattc cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt
    33181 ctaacggatc ccctttttgg ttcttctctg gtcgagcatg atagatcaaa atatcaaagc
    33241 acccgtcttc tgaaaccgta aacgagttgt gaccaggacc aaattgctta tttttttcag
    33301 aagaaacgaa tactggctcc gataatttat gccatgattc ttttaataaa agattcttgt
    33361 gctcatcagc ccacagcagg cccatggcat agttttcatc tgttgcacta ccagaatacg
    33421 taatgaatat tttcccattg cgagtaagga ctgctggacc ttcatttact gaaaaacccc
    33481 gtttttccca ttcatactca ggtatagaaa ggaggatctg cttccccttt aacgtccaag
    33541 gattatccat ttcggataga taaagattag aattcccagt aatttcagga tcttgttggg
    33601 cccacacata atatagcttt ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt
    33661 cccttttcgt ttttatttgt cccttttcta tccaatctgt attcattgga tctatatgct
    33721 gattttcaag tacgaacatg cggtgatgat gagattcatc acgtatagtt ttatgattgc
    33781 ttgcagcaaa gtaaatatac cattttccat tgatataatg gacttcaggt gcccagatca
    33841 attcactcat cggtccttct tcatgagcct gccatacaat caggcttgca gcatgttcta
    33901 attcattcag tgactttgcc cttcttaatt caatcacttg gtagcctgga accgatcctg
    33961 taaaataata ataaccatct gtatgtttat atacccaagg atctgcccgt tgccgaacga
    34021 taggattatt ataaagtgtc ttcatcataa caaatttcct cacattttct ttattctaat
    34081 tacattccaa gagtattttt ctatccctac atttaattcg tcatgttttt ctattgaaaa
    34141 atagtttatt tcaggtttta ttttttctgg ttcttcacgt gtatttttgt ccaacagatc
    34201 gctactggtt aatgtgtagt gctctccttg atctagcaaa ttgaaatcat gttcaatcgt
    34261 tatcttttgt tgttcatttg tgcgattgat caagaaaata gtcagctgat tgccgttatc
    34321 tacgatcaca ctgtccacat aaggaacatt ttttatcttt tcacaatcat aaacagggca
    34381 aatgatttgt tgtttcaata cttgaccttt agcatatttc gataagtgtt gcatcacata
    34441 ataagttgga ttcctccatg cttgtccttt agcatctgtc agtattaaag gtattgtgtt
    34501 tactaacaat gattgacaac taatctttac gcgatctgcg tgtcgtaaaa tagccagccc
    34561 taatgaacca aataataatg tatcttccat agtaaagtaa caccaatcga tgggactacc
    34621 gacttcaaaa tccctatgtc ttttttccgg ttgggcatgt acattccatt catcaaacgc
    34681 tagatacatt gttttctcac ttctcaaata tccttttaca taatcacaag tagcaacgat
    34741 ttcttcaatt tgacgatcga atcgataagc ttttgcaagg tatgtcgctg tatcatcacg
    34801 ctcattttta ggaggtttag tcaagtcttc ttcatcatac ttatcaatat agttatgcaa
    34861 agctaaataa tctacattct cataagcttc ttctaatgcg atacgatccc attcagggta
    34921 cgatggtaaa cgtggcgtag agcttccaac taagattgtt tcaattgaat catccactaa
    34981 cttcatgact tttgaggctt catttgctaa cctaccatat tcataagcac tcttagcccc
    35041 cacttgccat ggaccgtcta gctcattccc taaacaccat aatttaacgc caaaaggctt
    35101 tttttgccca ttttttattc ttaaatcact ccagaaggtt ccttttggaa aattacaata
    35161 ttctactaaa ttttttgctt catcaattcc ttttgttcct aaattcaccg tgaatatcgg
    35221 tttcgcttgt acatgactga tccatttaaa aaattcgtgg agtccaaaag aatttggttc
    35281 tatacctcgc catgctaaat caattttttt tggtcgttgt tctttcctac ctacagtatc
    35341 cacccaatca taaccagatg taaagtttcc tccaggatag cgaatgacac ctaattctaa
    35401 ttcctttaca taagagagga catcttgtct aaacccgtca ccatcagctg tttcatggcc
    35461 cggttcataa atccctgtat agacgacact tcccatatgc tcaacaaatg agccaaacag
    35521 ccgagaactg atctctccta ccgtaaaatc ctctctgatt tttactttca cttcttcgtc
    35581 caacagcttt ccctaccttt catttcctat agtcaagata atagatacaa gtttccatac
    35641 gccaccagca aagataaaaa taaaaggagg gaaaagaaat atgagcagta caaatcctaa
    35701 taacaatacg caacaaagaa tatttctcaa aaaatcaaaa aatactttcc atgcactttg
    35761 ttcaaaaatt tgtaaaaatg tgatatcttt gttttcagaa atcacatata tccaggataa
    35821 aagaaacatg gaataaataa gtaaaactac atatccacta attgaaagta cgattgtctg
    35881 ttcttctaat aattgattaa caagaaaata aataatgatc gttaacagac taaggaacaa
    35941 gttcttacca aaatgttgct ttattccttg aaagaaaaaa taaatgatat ttcctgatcc
    36001 ctcacctttc cattgcgcca ttacataaaa aacagcacat gtagcaggca cgatagtaat
    36061 aacaggcaaa cacacgatta cccagagaat acctaataag atgtaatcag aaaccacaat
    36121 caactgtcca taaaatttag tctcaactat tttcatcttc ctcactcttt cattccagac
    36181 atagctgttc cttgaacaaa atatttctgt gcaaaaatat aaaccactag taatggcagc
    36241 attgaaataa ccgttccagc cattacaggt ccataaaaag aaatgttctg ccctgtaaat
    36301 aaagctaacc ctgctggtaa agttctcatt tttgaactac ttgtcaatat caatggataa
    36361 agcaagtcat tccaactatt catcagcgtg aaaatcccta atgataaaag agtaggtttg
    36421 ctcaatggca acatgatttt caaaaaaata ttaaactcac ttaatccatc aatgcgtgct
    36481 gcctcttcca aagaatctgg taaagtaata aaaaaggaac gcatcatgaa gataccaaat
    36541 gcagttgtca tacgaggaat gatcaaacct gcataagtat caagcatgcc aagcaaattg
    36601 acttcaataa atagaggaat catgaagact tggaatggga tcatcattgt cagcaaaacg
    36661 aaatagaaca aaactgattt tcctttgaaa cgcatacgag caaatgcata ccccgcaaga
    36721 gaatcaaaga atacagaagt gatcactgtc cctccagcaa aaatcactgt atttttgatg
    36781 taatctaata aagggatcgt tgtccaaacc ttaatatagt tatctagggt atagctatct
    36841 gcaaatattc taggcggaaa tgagataatg tctttttcgt gtttaaaaga agaaataatc
    36901 agccacaaaa aaggaaacgc aataaccaac aatataacta gcatactaat cgttcgaatg
    36961 atttttttac cactaactgc ttttcgacta ttcacagtaa ctgtaatcga gctttctggt
    37021 gccatacagg tttccctcct atttcatttg tttttctttc cgtgacatgt acacattagt
    37081 ggagatagct atagttgcaa caatgataaa caaaaccgaa gacagtgctg atgcatagcc
    37141 caattcataa ggtgctgtaa atcctcgact gtaaatatat tgaacagctg tttctgtctt
    37201 aaattgtggg ccaccagcgg ttgcaacata tacttggtca aacacttgca tcgaaccaat
    37261 cagattcgtt aaaatacaaa aacctaagga gggaacgatc tgaggtagcg taatgttaaa
    37321 aaactgctgt cgatttgttg caccatccat ttgagcagat tcatacaaag agggagagat
    37381 tccctgaata gcagtcaaga gaatcgtcat agtaacacca aaattcttcc aaacagtcat
    37441 gatggctacc gtcggcatag caagggttgg atcgcgaaaa aattgaggga tcggcaagcc
    37501 gacttgtgaa aacaagtaag gaatcgcccc tacattcgga tcaagcaaca tagaaaaaat
    37561 aatcgcaatt gacgtcaatg aacaaataac tggaatgtaa aatgtcgaac gacaaaaacg
    37621 attgaatctg gtatttttag ataatgctac tgctgctaat aaaccaacga taatttgtgt
    37681 tggcgtttcg agtacggtga aatataaaga atgaatcaac gaattaactg ctcgttcatc
    37741 actaaatata cgtgtgaaat tttcaaaact tacaaaattt cttgatgtaa agaaaatatt
    37801 caaatctgta aaactaatag caaatgttcc taaaagcggt atgacagtaa ataataataa
    37861 aactaaaaaa gatggagtta aaaacaaata cgcagcacga tcaggacgat tccaatattt
    37921 ggtgaatccg ttcatatagt ccacctcatc tttcattttg ataaaatata caatcttggt
    37981 ttaaacgaat ctgaataaga tatccgaaca atcgctgtga tttttgcaat aacacctatc
    38041 tgcaaatact aggtcgatca ttcagatatc tgtatcttgc caacagtcta tcctgtataa
    38101 agttctgtac tatttttgca gtaattgatt gatggcatca tctgcatcgt tcatgagtgt
    38161 ctcaggatct tctcctgcta ataatttttc aatcagtgga ttgatgatat cgttattgat
    38221 cgcagagatt tgggtaaaac ctggtaaata tggctctgca tagtccattt gtttcgataa
    38281 ttcagataca ataggatcat tttttacttc ttcatctttt gctactgaat ccaagtaggc
    38341 aggaaaacca ttttcgatac tccatttttt accaatttca gttgtattcc aatatttcaa
    38401 aaactcatag acagcttctt ttttattatt atctgtactt gccggaatgg catatcctac
    38461 gccatctaaa atagctgttt ttttgtctga atcactccct tgtggtaagg tagtcacatt
    38521 ataatcaatc tcgtttgctt tcaatccgtt atttaaccat ggcccattga tttcaatggc
    38581 tagttgacca gcattcatta aattgtccat ttcggcgcca gaaatcgatt ctggaccagt
    38641 cttgtctaca tggatcattt tttggatcgc ttttaacgtt ttaaggttct caggagacgc
    38701 aaattcagac ttagtataat catcgttcac taatttaccg ccatttgcta acatccagtt
    38761 atataacggt gcagttccat ccttattgaa gacaaaacct gatacatttt tactactatc
    38821 agttaatttt ggtgccattt ctgtcaattc ttcccaagta gtaggtggtt gttcagtatc
    38881 taatccagct tgtttaaaca aatcattatt ccaatacata tacatgcctt gtacctgcat
    38941 ggggacaaaa tactgtttac cttctacttc acctaattca atagccgttt ctttaaaatc
    39001 agatttatct actccatcat attcccagaa atcgtctaat ggttgtaccg ctccgttttc
    39061 tacatactgt gcaaaatctg gataatttaa tgcaataaag tcaggtgctt ttttagaaga
    39121 aatcgctgta ggaagttttt cattcaaatt ggcccaagtc atgatatcca tttcaatttt
    39181 gatgttttta tcatttgttt tattaaattc attaacgatt tcttgcaata tttcaccatc
    39241 agatgcagta aacccattcc aaaaagtcaa agtcacttct ttatcagaat cagattctgc
    39301 tgaacttccg ccaccgcaag ctcctaaaat acctgttgca gctaccaaca aagagccaat
    39361 taaccatttt ttacctttca ttttttgttc ctccttcatc tgataaacga catctgacca
    39421 cattccaaga tagcggtttc aataaacaat tgattttccc atcttcagct tttacatttg
    39481 ttgaatgact tggttttact tgttcacttt taggaagatt agttgctttt gtatcaaatc
    39541 cactcatttc agaaaaatca atcactccct ctaatgataa ttcagacaag tgaacagtta
    39601 gattcatttc ttcttctcca cggttaactg caaatatgac aagttctttg tcatttatta
    39661 cggctatact ttctaaatac ggcacttctt caaaatcttc cgtcgcatag tttgctgaac
    39721 gaatttgtgg gcttaacacc tctccttgtc catacacagc agcttgcata aatggataat
    39781 agatcgtctg tctccaagct tctccgtttg tttctgtcct gattggtgca ataacgttta
    39841 ccaattgagc aagacaagca atctttactc tatctgcatg tttcaataaa gtgattaata
    39901 gacagcctaa tagtaatgca tcttcaaaat tatagttatc ttctagtaaa ggaggagcaa
    39961 tctgccaagg cgctgcttgc tcatcttgtt ccgctgaatg ataccaaata ttccattcat
    40021 caaaggacag gttgatctgc ttcttactat gtttcttcgc cttgactgta tcgcacatcg
    40081 ctgtcacttc agaaatgaac cgatccatgt ctaaggaacg tgcaagataa tgagaaagat
    40141 catttttttg gttgccataa tattgatgca gagacaaata atctatttga tcgtacgctt
    40201 gatcaagaac tgttaactcc caatcaccaa atgtaggcat ttgccgattc gaacttccgc
    40261 ataaaaccac ctcaattgaa tcatctacga gtttcatcgc cttagccgtt tcctctgcta
    40321 accgtccata ttcttccgct gttttatgtc caatctgcca aggaccgtcc atttcattcc
    40381 ctaaacacca tgtcttaatt ccaaaagggc tctcttgacc attcgaacgc cttaaatcac
    40441 tccaataagt tcctttagga aaattacaat attcgactaa attacgagct gcatctactc
    40501 cacgagtacc cagattgaca gccatattga tttcggcccc cacatgctga caccagttat
    40561 aaaattcatg aatcccaact tcattcgttt caattgtccg ccaagctaaa tccaaccttc
    40621 taggtctatt tgctatgggc ccaatactgt cttcccaacg aaatcctgac acaaaatttc
    40681 caccaggata tctaatcatg ggaatgttca attcttttac caagtgaatg acgtcttggc
    40741 gaaatccact tttattagct gatggatgat ctggctgata aatcccttca tatactgctc
    40801 gtcccatatg ttcaataaat gaactgtaca accgcggatc aactttgcct acacgcttga
    40861 cactactaat caacattgtg gcttccataa ctgacctcct ttaatttata catgaataat
    40921 acacctcgtt gaaagcgttg tcaataatat aaatgttaaa cttacttatt ttttatcgat
    40981 tttaattaac caaaatatta ataatgatta aaacaaataa tttaatataa cttttattaa
    41041 caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca actacaaata
    41101 aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat caagatcatc
    41161 cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact tggagtgagc
    41221 agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat caaaacagaa
    41281 aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat tagttttcca
    41341 gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg tcactataca
    41401 aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat cgggaaagtg
    41461 gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct ctggtttacc
    41521 agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac attggagatg
    41581 ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa ttggccttca
    41641 gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc tggagatttt
    41701 gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa tcaatacggc
    41761 ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga acccttgtca
    41821 aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg aatcgaagtg
    41881 aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt cggtaattac
    41941 gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct attttataaa
    42001 acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt atttccagaa
    42061 atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc accatgcttc
    42121 gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat caatttcatt
    42181 atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt caagatctgc
    42241 agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca attgtttccc
    42301 ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac caaatttcgt
    42361 gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc ctgctctttc
    42421 tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc ctttgttaaa
    42481 atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa cagctggttt
    42541 gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt ttgcatcttc
    42601 ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt tttcatcttt
    42661 tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta aagtagaatc
    42721 ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt tcaaatatgc
    42781 tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg gtttgacacc
    42841 taattcattg ataatttctg tctgcaggct catttattct cctcctatgc ttctgtatga
    42901 agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt ccagcattca
    42961 gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc ttcccatagt
    43021 gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa ttcgtaaact
    43081 ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc tcgaacagtt
    43141 ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc tcgagggtct
    43201 tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg agtgatgcgc
    43261 cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa cttgatcgta
    43321 tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc accaagggct
    43381 ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat tttagctttt
    43441 tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata gatttttgct
    43501 tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata ggccatatct
    43561 ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt cgccacgcga
    43621 atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa cacgcagtct
    43681 ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga ttggactaac
    43741 gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg tacattgctt
    43801 gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc atccagttct
    43861 tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga tttgatacga
    43921 gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc tgtttctact
    43981 aattggcagt gcgctaaagg cccttccact tggatcaatg gttcatttgc aaaaactagt
    44041 tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa atactccaaa
    44101 aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc attgaacgtc
    44161 agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta tccgtggtta
    44221 aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg acctaattcc
    44281 cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa actgtcgtct
    44341 gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc ctacccatta
    44401 taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag cacatgtttt
    44461 acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc ttattggtat
    44521 ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac gaagaaatta
    44581 gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca atcgtagccg
    44641 ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa tcaatggcaa
    44701 aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa agaaacggat
    44761 ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat ataccttgcg
    44821 ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag taagaaagca
    44881 gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc ttgtttttta
    44941 ttgtatacat tagatatgga gcaggttacc ataaagatga ttattagtcg ttattaaata
    45001 gaatccttcg tatttttttg ttctatcact acaatatctt tatttggacg tttgaattgt
    45061 attccggcac gttctagctc ctgtttcact cctacatgga ctttgacatc ttctcgtgtc
    45121 atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt tttcttcgta
    45181 ttgatcatca cgacttgctt tttatcatat gtgtctacca atagcagttg tgttcgatct
    45241 aacgaaaata atttgtaata attaatagcc gtattcccaa aaaagaattc cactacttta
    45301 ggataaaatg tcttaatatt taaattacta ttcacaggtg tttcgattag cttcatctca
    45361 ttcctctttt ctatatagtc gatttagatc caatcttaag ttatgttacc atctttccta
    45421 gcattaagga atcggttaca attgaacctt taaagacaaa aggatttacc ttttatcact
    45481 taatagtata acataatttc atgagaaaaa aatacaattt tttcttgacc atctcgttac
    45541 gtaaaggatt atgcttacat ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa
    45601 tgtcagagat atcaggagtc agtccgagaa ccttgcgctt ttatgatgag attggactgc
    45661 taaaaccagc taggatcaat tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc
    45721 gtctgcaaca catcttattt tatcgaacaa tggcattcaa gttggatgat atccaagagg
    45781 tcttagacaa tccatctttt gaccaccaga aagctttaat caagcatcag caaatgttgc
    45841 tggaaaaacg ggcacaaatc gataccctct tgacaactgt ccaacagaca cttgatatgt
    45901 acgaaggagg aagaaaaatg agtgacaaag aaaaatttga agggtttaaa caacaaaagt
    45961 tgaaagaaaa tgaagaatcc tatggacaag agattcgaca aaaatacgga aaaaaaaagg
    46021 ttacagcatc caatgaaaaa tggatgaata tggataaaga aacgtatgaa gaaatgcaaa
    46081 ctgttgaaaa gcagatgtta tttgatctca ctctttactt aaaagaacca gaagatgaaa
    46141 gcttagcaga ccgtatcttc caagctcata agaagtggtt atcttacagc tggcctgaat
    46201 atcagccaaa agcgcataaa ggactaggaa tgatgtacga agccgatgaa cggttcactg
    46261 catattatga cgagcgaagt ggtcatggtg cagcaaaagc tttaaatgaa attatccaaa
    46321 aaaaggcgta atcttttaca tgatccaaca aaaaagtaca cgtaaaatga cctaataaca
    46381 aaacagcaga cacccaagtt agatttttat gctaactttt ggtgtctgct ccattctttc
    46441 acaagttttt tcatcctact ttaaatatat tcatcgttga ataaacgtat aaggtatttt
    46501 cactttatcc tttttttctg taagaaacaa ttgaaatgcc atttctccgc aagcttccag
    46561 atggtggtcg atggttggga aaccaagaag ctcgctgatc agcaaattat cgcgtccaat
    46621 caccactggt gtcttatctt gcagatagct ttgcaagatg cctgctgcaa ccatatccga
    46681 atttgtaaag acaccatcaa ctccaagctc tttaaaaaag aagcctgctt gttttccttt
    46741 ctctgcatct atacaatccc aaaaaacaag tgattcatca aaagatggaa aaagttgatg
    46801 acatagctgc aaagtgattt ttgagttata acttagcctg ccacttcgcc ccaacgtgat
    46861 gcccaattgc tttatacctt gctgcttcaa ataagtaaga ccttctgtaa ttgattgttc
    46921 tcgatcaata aagacacagc ttgcctgttt tcccttgatt tcttcacaaa aaactagagg
    46981 tccatatttt tgatatgcca ataaatcatc tagtgtattg gcacgagacg taatgatcaa
    47041 tccatcgaaa gctttagctg caaattcttc aagatacctt cgctcttttt ctttctgata
    47101 gcccgtcggt aaaagtgtca ctttgtaatc ataatcaaac gctgcttcaa tgatcccact
    47161 gatcaactga tcaaaatacg gatgattggt ataaggcaga atcactccaa tgcttttcgt
    47221 ttttccataa cttaactgtc tggcatttgc attagggata taatcgactt ctttcatgac
    47281 cgcaagtatt tctttccttt tttcctcatc tacatatgga tgtccattga ttacacgtga
    47341 aatagtagaa actgaataac ctgtgagctt tgcgatatct cgaatgtttg ccactgctgc
    47401 ttcgccacct atacaaaaag attttcttgt aaatagtcta ccacagctgg accgcattca
    47461 aaaaactgat acatttttcc ttcatgcgaa aaactgatcc gctgcccgcc ccaatatata
    47521 tcttttgtca aattttcgat ttcattcatt ggtatttctt ttactatatt cctattcaac
    47581 ccttgaaaac tgtacaacgt taatacggat cccttgaact cgacaaacgc ataaaaataa
    47641 ttgttcgttg tgccatctgt tatctctaca taagtattca ttgcaagccc tccttttgat
    47701 actttgagta tagtgtatga aacgcgtctc atagcaagga gtttgttttg ccaaagaaaa
    47761 gttttattcc tctttcatta ttaccaaagt ctcttatttt aaattgaata cattataatg
    47821 atactgtaaa tatatcttta ttattagtga atggatattt a
//
LOCUS       DACEQH010000019        47112 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.019, whole genome shotgun sequence.
ACCESSION   DACEQH010000019 DACEQH010000000
VERSION     DACEQH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.019"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(137..1528)
                     /locus_tag="IW833_001087"
     CDS             complement(137..1528)
                     /locus_tag="IW833_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ1307218.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1741..2127)
                     /locus_tag="IW833_001088"
     CDS             complement(1741..2127)
                     /locus_tag="IW833_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ1307219.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2183..2806)
                     /locus_tag="IW833_001089"
     CDS             complement(2183..2806)
                     /locus_tag="IW833_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ1307220.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2870..3538)
                     /locus_tag="IW833_001090"
     CDS             complement(2870..3538)
                     /locus_tag="IW833_001090"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1307221.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3550..3990)
                     /locus_tag="IW833_001091"
     CDS             complement(3550..3990)
                     /locus_tag="IW833_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307222.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4049..4720)
                     /locus_tag="IW833_001092"
     CDS             complement(4049..4720)
                     /locus_tag="IW833_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1307223.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4905..5303)
                     /locus_tag="IW833_001093"
     CDS             complement(4905..5303)
                     /locus_tag="IW833_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ1307224.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5300..6073)
                     /locus_tag="IW833_001094"
     CDS             complement(5300..6073)
                     /locus_tag="IW833_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1307225.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6070..6753)
                     /locus_tag="IW833_001095"
     CDS             complement(6070..6753)
                     /locus_tag="IW833_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1307226.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6920..7807)
                     /locus_tag="IW833_001096"
     CDS             complement(6920..7807)
                     /locus_tag="IW833_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1307227.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7804..8673)
                     /locus_tag="IW833_001097"
     CDS             complement(7804..8673)
                     /locus_tag="IW833_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1307228.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8810..10096)
                     /locus_tag="IW833_001098"
     CDS             complement(8810..10096)
                     /locus_tag="IW833_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1307229.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10369..10947
                     /locus_tag="IW833_001099"
     CDS             10369..10947
                     /locus_tag="IW833_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1307230.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            10947..11876
                     /locus_tag="IW833_001100"
     CDS             10947..11876
                     /locus_tag="IW833_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1307231.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11873..13609
                     /locus_tag="IW833_001101"
     CDS             11873..13609
                     /locus_tag="IW833_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1307232.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13672..14508)
                     /gene="pheA"
                     /locus_tag="IW833_001102"
     CDS             complement(13672..14508)
                     /gene="pheA"
                     /locus_tag="IW833_001102"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ1307233.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14505..15014)
                     /locus_tag="IW833_001103"
     CDS             complement(14505..15014)
                     /locus_tag="IW833_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ1307234.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15007..16302)
                     /gene="aroA"
                     /locus_tag="IW833_001104"
     CDS             complement(15007..16302)
                     /gene="aroA"
                     /locus_tag="IW833_001104"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1307235.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16315..17406)
                     /locus_tag="IW833_001105"
     CDS             complement(16315..17406)
                     /locus_tag="IW833_001105"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ1307236.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17423..18589)
                     /gene="aroC"
                     /locus_tag="IW833_001106"
     CDS             complement(17423..18589)
                     /gene="aroC"
                     /locus_tag="IW833_001106"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ1307237.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18591..19655)
                     /gene="aroB"
                     /locus_tag="IW833_001107"
     CDS             complement(18591..19655)
                     /gene="aroB"
                     /locus_tag="IW833_001107"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ1307238.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19688..20710)
                     /gene="aroF"
                     /locus_tag="IW833_001108"
     CDS             complement(19688..20710)
                     /gene="aroF"
                     /locus_tag="IW833_001108"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ1307239.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21369..22343)
                     /locus_tag="IW833_001109"
     CDS             complement(21369..22343)
                     /locus_tag="IW833_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ1307240.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22324..24207)
                     /locus_tag="IW833_001110"
     CDS             complement(22324..24207)
                     /locus_tag="IW833_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ1307241.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24225..26450)
                     /locus_tag="IW833_001111"
     CDS             complement(24225..26450)
                     /locus_tag="IW833_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ1307242.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26564..27598
                     /locus_tag="IW833_001112"
     CDS             26564..27598
                     /locus_tag="IW833_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1307243.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27665..28678)
                     /locus_tag="IW833_001113"
     CDS             complement(27665..28678)
                     /locus_tag="IW833_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1307244.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28756..28944)
                     /locus_tag="IW833_001114"
     CDS             complement(28756..28944)
                     /locus_tag="IW833_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ1307245.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29384..29512)
                     /locus_tag="IW833_001115"
     CDS             complement(29384..29512)
                     /locus_tag="IW833_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1307246.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29515..30888)
                     /gene="rlmD"
                     /locus_tag="IW833_001116"
     CDS             complement(29515..30888)
                     /gene="rlmD"
                     /locus_tag="IW833_001116"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1307247.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(30999..32054)
                     /locus_tag="IW833_001117"
     CDS             complement(30999..32054)
                     /locus_tag="IW833_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1307248.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32064..33494)
                     /gene="gatB"
                     /locus_tag="IW833_001118"
     CDS             complement(32064..33494)
                     /gene="gatB"
                     /locus_tag="IW833_001118"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ1307249.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33491..34963)
                     /gene="gatA"
                     /locus_tag="IW833_001119"
     CDS             complement(33491..34963)
                     /gene="gatA"
                     /locus_tag="IW833_001119"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ1307250.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(34963..35268)
                     /gene="gatC"
                     /locus_tag="IW833_001120"
     CDS             complement(34963..35268)
                     /gene="gatC"
                     /locus_tag="IW833_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ1307251.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35374..37410)
                     /gene="ligA"
                     /locus_tag="IW833_001121"
     CDS             complement(35374..37410)
                     /gene="ligA"
                     /locus_tag="IW833_001121"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ1307252.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37427..39661)
                     /gene="pcrA"
                     /locus_tag="IW833_001122"
     CDS             complement(37427..39661)
                     /gene="pcrA"
                     /locus_tag="IW833_001122"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ1307253.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            39816..41375
                     /locus_tag="IW833_001123"
     CDS             39816..41375
                     /locus_tag="IW833_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ1307254.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41486..43417)
                     /locus_tag="IW833_001124"
     CDS             complement(41486..43417)
                     /locus_tag="IW833_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1307255.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43435..44349)
                     /gene="pfkB"
                     /locus_tag="IW833_001125"
     CDS             complement(43435..44349)
                     /gene="pfkB"
                     /locus_tag="IW833_001125"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1307256.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44346..45098)
                     /locus_tag="IW833_001126"
     CDS             complement(44346..45098)
                     /locus_tag="IW833_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1307257.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45258..45467)
                     /locus_tag="IW833_001127"
     CDS             complement(45258..45467)
                     /locus_tag="IW833_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307258.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45478..46806)
                     /locus_tag="IW833_001128"
     CDS             complement(45478..46806)
                     /locus_tag="IW833_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307259.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT        13143 a        10183 c         8400 g        15386 t
ORIGIN      
        1 caacctctta ttcacggtgt ttcagaagta gcttctcgga aataagccga aatttcacaa
       61 aaatttgaga aactattttt aggaaacttc ttcttatttc ttaaagctga ccacttctat
      121 cacaacctct ttttgactat gcattcacat cgatcacttt agaatcatca ttgatgacat
      181 cgcgatctaa atctttgatg cgtgcacttg tcacgatacc agctaccatg ctaccgctga
      241 cgtttactgc tgtacgtgcc atatcgatta atggttcaac agagataact aacccaacga
      301 ttgctactgg caagttcatt gcccctaaaa cgatcagtga agcaaatgtt gcgcccccac
      361 cgactccagc tacaccaaaa gaactgattg ttacaattgc cacaagcatc aaaataaatt
      421 ccaaactaaa gacattcact ccagctgctg gtgcgacgat cgttgccaac atagctggat
      481 aaacgccagc acagccgttt tgtccaatag acaaaccaaa acttgctgag aaattagctg
      541 ttgcatcatc tactcctaaa gcttttgtct gagtctcgat attcaatggc aaagctccag
      601 cacttgaacg tgaagtaaac gcaaagctca ataccggaaa ggcttttttg aaatagttga
      661 ttggattgac ttttgcacca atcaaaatca gtgagtggac aatcaaaaca gcaagcaatg
      721 ctacgtaaga agcaacgaca aatttaccta aattgacgat agctgcaaaa tcacttgttg
      781 ccaaaacgtt tgtcatcaaa gcaaatactc cataaggtgt caaacgcaaa acaagcgtca
      841 cgatccgcat aatgatttta tacagactgt cgactaaatc tgaaaagaat gctgcttctt
      901 ttggcgcttt tcttttcacg cctagataag cgattccgac aaatgccgcg aaaataacga
      961 ctccaatcgt acttgttgca cgactacctg ctaaatcagc aaaaacgttt ttagggataa
     1021 aggataaaat ctgctgtgga atcgataagt tttcaactgt ttcctgacgt gttgccagtt
     1081 cagagattcg tgctgtttct gcagctcctt gtgtgaattt agctccttgg agtccgaaca
     1141 gcattgttga accaattccg atcaatgctg caatagctgt tgttccaagt aaagaagtca
     1201 atacagtcgc actaattttc cctagctttt cagaaacttt catttttgtg aatgcaccaa
     1261 tgatcgaaat aaacactaaa ggcataacta gcatttgcaa gaatgataca tatccttgac
     1321 ctacaatccc gatccattcc attgattgag taacgacttt gtcgtctaca ccaaagatca
     1381 attgaagcag accgccaaag acgataccaa ctcctaatgc tgtaaagacg cgtgttgaaa
     1441 attttgtatg gcgcttttgc atacgataga acacatataa taatgccgca aaaactagaa
     1501 ccactaaaac tgtgatgagt gttgtcattt ttctcctcct catattatta atgacaagtc
     1561 catcgaagga agtaaaggtt cccgagaatg attcttcaac catcctcctg aaaaaatttt
     1621 tttcagtgac ttttatattt tagacgattt ttatcgaaat gacattcttt ttgcttacaa
     1681 ttagtaataa aaaaacatac ctgtgatagc tttaatctat cgcaggtatg tttttactgt
     1741 ctattcttgt ttttctgatt gaaaattcaa ttttacccca ggctgatcta aggcgatctc
     1801 tgtcacctca taagacattc ggcgtacgat atcgaataac ggtgctacta gctcgtccac
     1861 ttcttcttgt gtgacatcat tttgaccttc aattttccga ttgaggatat gattgatctg
     1921 actcacactt ccagataaaa catatgtatc aaacactaaa cggaactcta agcgtgctcc
     1981 tacgatgcta tgttctttcg gatagttttc gatcgtttct tttaaaggcg agaagcctac
     2041 tttcaaatct gtttctactt cttggttcgg caatctcata tcataatgaa atgcttctac
     2101 aacttctttt tgtcttctga tttccatata attttatcac tcctttatga aaattataac
     2161 atatctcctt tgaaagggga tactactttt taatgatgct ctgcaaggta aggatttcat
     2221 tcagatttcc taccacataa tctgccggga tctcacccag aaaatgatcg atgtcgtaaa
     2281 gacaagtagc gactcctgca tttttccctg cttcgatatc taaacgacga tcgccgatca
     2341 tcatcgtgtc tgttctttct aaatgaaaag tatcgatcaa ataatttaaa gatgctggat
     2401 caggtttacg aggaaagtcc tgatcgatcc ctactacttc ctcaatcaga tgtgccaatc
     2461 gatgttcttt taacaggccc catgttgatt ctgtcaaacg gtgggtcagg atgaaatgac
     2521 gaccgccgtt gtcttttagc atttccagta cttgtttcgt ttctggaaat ggacgggaaa
     2581 cttccgtact ttctttttca tatcggtgaa ataattcatt aaactctctg gcattcaagt
     2641 ttgattcatt gatcaaataa ctagtagaat actttttcat tttgaagtag atttcttttt
     2701 tgtccatact gataccaaaa tcttttagtg cttgccatgc accatctacc attgcgggat
     2761 acgtatcaaa caatgtacca tcaaagtccc atatataatt tttcatgttc cttttgttcc
     2821 attcctttgc ttcttttttc tatctctcac ttttagataa aggtgttctt taaagtattt
     2881 ttttgtccag aattgtcaaa tcatctgcta atgtatactg gattggttgt gcatttggga
     2941 tatcgatcgt atccatttga tcttcaggaa catcttctaa atattttgtc agtgctcgga
     3001 tgctattacc atgacccaca actagtaaat cttgccgatc ttttagtttt ggtgctagat
     3061 gatcttgcca taagggaatg atccgattca ctgtcatttc cagactttct ccgtatggga
     3121 tcaaatgcgg atccaagtga tcatatctgc ggtcaaatct attttctgcg gttaacggcg
     3181 gaacttccgt atatcctctg cgccatgttt ttacttgatc ttttccgtat atttctttca
     3241 tcttatcttt gtttttaccc acaagatccc cataatgccg ttcatttaga cgccatgttt
     3301 ttattatagg aagccataac tgacccattt cttctagtgc gacttgtccg gtaagaatcg
     3361 cacgtttcaa aacagaggta taaacggcat cgaattgaag tccggcttca agcatttttt
     3421 gaccggcaac tgctgcttgt tcttttcctt tttctgttaa ggcgacatct agccatcctg
     3481 tccagtaatt ttcaaaattt gcttcgcttt cgccatgacg taataatact aatttcattc
     3541 ttttctccct taaacattcg tttcgaattt tataccacat gttttgcatg tcacacgtat
     3601 tcgtcctttt cctttaggtg cacgctgctt ttgatgacaa ttcgggcact caaaaaaagt
     3661 ataacttttt cgatccattt tgacttgctt tttgtttttt atctgatgat atttttcttt
     3721 gactctatca agatatttca aataacgttg attctcattt agtctggggt agattttttt
     3781 tgataggaaa cgaaatatca tccatccgaa aaagacgatg aacaacaatt gtccgattct
     3841 atagggcaac caacgactta gcaaactaaa gacaagcgca gcgatcaata gaccgcgatt
     3901 caactgatca aatttcgcat atcttccctg cataagtttg tgatagttct gctgaaatcg
     3961 tattagcctt tctaaccatg tttgcatcat attatctcct ccatcatcta atatttatta
     4021 tgacaaaaaa atccaaaaag aaaaagcctt aattggcctt ttccattgca acttggtgaa
     4081 ggacattccg atttctggta aagtcaatca caatcacttt ctttttatct tctctgttga
     4141 ctactcgaaa tgtgtccgtc aatggaagaa acagcttctt agatggtagc tgttgcatac
     4201 gtataaaacg atggatcatc gtaatcaatt gaggaaattg aatagacgtg tgaagcagac
     4261 ctggatattt tttcaacagc tgtgtcgttt tgatcagatt ccgtttttta ctgatcactt
     4321 ttaaaaagct ttttagaatc ttttgctgac gtctaaaaat agaaaaagaa ttttcgtcat
     4381 ctatttgata acgcatttgt ttttgcagtt ctgctgcagt cagatggatc ttcccatcgt
     4441 ttgtatcgat cattaaacct tctggaaaca gttcaaccac tgctcgatcc caagattcaa
     4501 gagaaagctc gatataatct tttactggta attccaattc taccttaaaa aagtgcaaaa
     4561 cagccgacag cccttctgct tgatagattt ctcctaatgt tagcccctct agatggaact
     4621 ctggtgaaaa atgcagcaat gttttatttt cctgcaattg aacatataga aatgtagatc
     4681 gctcaatcag agtatctttt aaaacaacta aaaaattcat ttttctcccc cttttttcat
     4741 catttttaaa atagcatatt ttgaaaagaa agtctgatgc ttagagtaga atttcataaa
     4801 aatttactat ttcataagta ttcacgagaa aaatacaaac aaaaaagcgg aaataacgct
     4861 catccgcctt cttgtttgtg tttatttcct cttcaaacgc ttgtctatga ttcagacttt
     4921 tctctatcgt tattttcatc taaaagttct tgcattccga taatttctcg atattgatga
     4981 atcttagtga taccaatgac tttgtctaca tcaatactaa aacaaatacc attacctgga
     5041 atatcgatgt tgatgccttg accaatcgca tccattactt tattcgtcaa actatcgggt
     5101 actaatgtca acacaatatc tttttctggc gaaatgtcca aacctaaaaa tttccctgaa
     5161 tcatgtacac cagaacctct tccatgtaag atcgttccgc ctgctgcacc tgcttctttt
     5221 gaaaagtcga taacatcttc gcccattcct ccgtcaacga tcgtcacaat catgtgaaga
     5281 ttcaatgcta caagatcttt catttgcttt cccccaattt ttgcaatctt tctttttcga
     5341 ttctgtcttt ttcatctttt cgtttataaa tatatcctag tactaaagtg gataatattg
     5401 gtgtcaatgc aatcattgca accacaccaa acccgtcaat gatcggatct cttccttcaa
     5461 tttgtgcagc gattgccacg ctcattgata aaataaatgt cgaacacatc gggcccgttg
     5521 ctactccacc gttatcaaaa gccattgcta aaaagatctt gtccactttt cgtcctaaaa
     5581 taaatgctag cccgtatcct gggatcaaga aataataaag agagaatccg acgagcaaac
     5641 gccacataga caatcctact gctgcgccga tcccaattga aattaccatc agcatgattt
     5701 tcgatcggat tctcccctcg ctcaattctt caacctgttt gatcattaca tgaatcgccg
     5761 gctcagcaaa ccctactaag aaccccatta aaaagccaag gggaaccagt atataattgt
     5821 aaggcaatcc cgcaatagca ctgcctaaat gctgtcctac cggtacataa gccatattga
     5881 ctccgtgcag gaaaaggatc aacccaatag tcgttatcaa gaaacctttc ataatgtcaa
     5941 agaaacgctt tttgcgcata cgaaaactaa tgatattcat taaaacaaat aggattacga
     6001 taggtgccac tgccaaaatg acttccatgg taacgatttc gattccatct atcacttgct
     6061 gccagaaact cattggaaga tcacccccaa aatcatcacg gcaagaatcg gccctaacga
     6121 agcaataccg accattccaa aactgtcatt ttcatttttc ttttgtttga tcatactagt
     6181 catccctccg gcaagggcca agataaatgg aacagttact ggaccagtag tcactccccc
     6241 cgaatcaaaa gcaatcggca taaattcgga actggtgaaa aatgaggcga tcaaaacgcc
     6301 tatgtagcct atagccatca attgataata ggatagcttg aatacgaccc gaagcagcgc
     6361 aaaggctaga aatactccag tgccgacgct gacgatccca atcagcaaca ctctgccaat
     6421 ctctccgtca gatatatcat atacttgttg tcccaaaact tgcacggaag gttcagcaat
     6481 cgtgatcacg atcccgatag caaatcccaa actgaccaaa acggtcaaac ttttcttttt
     6541 gatcatatat ttccctacta gatctccgac ttccatcata gaataatctg ctccaaacaa
     6601 aaagagcgtc attccgatca tcatgattgc cgcaccaacc agaaatgaag cgaggtcttc
     6661 tccttctaac ggtgcaaaaa caaacgttaa aatgacaatc aggacagtca tcggcaatat
     6721 agcagcaatc acttctttaa aattttttag catgtatcct cctcctttac tctttaagtt
     6781 taccttaaaa tgaaagagtt ttcgagcaca aacttaaaac gttatcattt aatttttctc
     6841 aacataaaat aacacacgtc ccaaaagtat ctttttactt ggaaaaatga ctttgaagga
     6901 acgcgcttat tgtgtttttt tattttacag agccgatcat tccttgaacg aattgctttt
     6961 gcaaaacaaa aaatacgatg gcagttggta aagtagcgat gactaatcca gccatgatca
     7021 ttccatagtc tggtgtatag gaagcaccca tagcagataa aacaagcgga accgtccgtt
     7081 tatcaggaga ttgcaaagct actaagggcc ataaataatt gttccagcta ctcatgaatg
     7141 tgatgattgc agccgcggca taggtatttt tagccgtcgg catatacact ctaaaaaaaa
     7201 tacttagttc ttttagaccg tctaatcgag cggcttctac tagatctttt ggaaaagatt
     7261 taacattttg tcgaaagaag aagatcaaaa aagcagtgct aacagatggc aggatgacaa
     7321 ccaagaaact gttgattccc aaaaccgttc cgttcaattg tgaaaacatt cgatataaag
     7381 ggatcatcaa tgccgcaaaa ggaaccatca tcgatagcag tagaatattg aaaattaggt
     7441 cttttcgttt actttgataa atctcaaatc catatcctgc cattgacgaa atcagcaatg
     7501 caaacacagt ggtcaccaaa gcgatcatcg ctgaattcca aagggaacga gtaaaattca
     7561 gctcattgga aaacaaattt tgaaaattga tgatcaagtt actgccaatc tttatttttc
     7621 ccgatgtgat atccacgggt gtattcgtca ttcccaaaac catccagaca aaagggaaaa
     7681 tggaaataaa tgataggatg cttaatgctg tatactttaa tactgaccca attgtcgaac
     7741 gaagttctaa tgcttgtttt ttgacgttgg ggcgttcgat tgtttctttt tctaccatat
     7801 ttttcatcga ctttctcctc ctactttcgt ttggattatc gacaatatca caataaagga
     7861 aacaataaca aaggaaactg ctgccgcata tccaaaatta ggcgtgaatt tataactcaa
     7921 attatagatg tattgagata gggtggtcgt tgcatttgct ggtcccccgc cagtaatatt
     7981 ttgtacttca tcaaaaagct gcaatgtccc gatcgtcgaa gtaatggaag taaacaaaat
     8041 gattggtttt aaatttggga tcgtcacttt gataaactgc tgccatttag aagcaccgtc
     8101 aatagatgct gcttcataaa tctccgaatc aatattttgc atgcctgaaa ggaaaaaaat
     8161 catattgtat ccagtccaac gccaagtgat cgaaagaatg atcagaactc tcgcccaaat
     8221 tggatgagtc aaccagccaa tcggtgtgtc aatcatacca atgcccaaca acaagttgtt
     8281 tacaaggcca gattgagaaa atagactttt catgatcaga gaataactga ccatcgaagt
     8341 gatacatgga agaaagatag cggttcgaaa caacccttta tatttcaact ttttatcatt
     8401 taaaaggttg gaaataaaaa gtgctagaaa caacatgatc ggtacttgta caagtaaata
     8461 caggaatgta ttaaaaaaag cttttttaaa agttgcatct gtcaacatcc gctgataatt
     8521 acctaacccg ttaaatgtca tattgacagg gggaccactt tgaaaagacg tgatcaaagc
     8581 caaaaccatc ggaataaaaa ccatgattga tatcaaaatg actggcaaga ttaaaaaagc
     8641 atgcccgttt ttgaataaat ctttctgctt cattcttttc cctccaattc tatcattgat
     8701 tatctacatg aaaagacaag aaagacaata tctgtaaggc agacgtgatc ttttttgtct
     8761 tttcgtttgt ctgatgaaaa agcgaagaaa ttcacttttt taccaccgat caattagcca
     8821 attgtgcttc tacttgtttt tgtgtatctg ctagtacctt gtctgtctct tctccattga
     8881 tgattcgatg aagagcttca gctacgactg cttcaattgc ataggtctca tgtccgtaat
     8941 tcacttctgg tatttcagat gtccatgttg aaaaatcgtc aaagatcttt tgaccactgt
     9001 aaaattcaga agcttcttga taagcttctt gttcattcgc gctaagcatc gtagatacta
     9061 gtccgatttc tttcgcaaga gtgcccatca gttcttcatt cgaagcaaac gttgcctcta
     9121 ggaaatcttt cgctgaatct tctccagcta ctcctttgat cacataccag ccagcgccgc
     9181 caaggttaga agcttgcgct ttttgcagat tttctggaag tgcaggtatt ggtgcaatct
     9241 tccattttcc agattggttt tctgctcctt ggatcgttga agaataccaa gcgccagttg
     9301 ggctagaagc tactgctcct gattgtatcg cagttactcc agcattccag tcagaggttt
     9361 gttcgactag atcttctttc aaaagagtgg caaacaattc aagaccatac tttaaagatg
     9421 cattgtcttg gatcgtgact gtctccccat cttcagatgt ataccattct cctgcctgtt
     9481 gcataatcat ccttactcga ccaaggtcag aaggattgac ttcagcgatt ttctttcccg
     9541 tcttttcttt gacatctcgc gctacttgaa tatactcgtc ccacttcaga ttctccatat
     9601 cctcttctgt ataacctgct tctgccatca gatccgtacg atagaagttt gctgttacgc
     9661 ccgaatcgaa aggaacaccg tatattttat ctcctacttt gtttaccgca aatttatacg
     9721 gtgcaaaatg atcttccttt acaatagcag acaaattagc gaatgcatct ggataagaag
     9781 tcaaatagcc ttgtatccta taatcttcaa tcaagacgat attgggcaat ccttctgtat
     9841 tcccgctcgc taaagcggtg ttcagtttct gaacgatatc atcctgagac atcgtaacaa
     9901 cttctatctc tgtttcttcg ttctcataaa ctttttttgc ttcattcact gctttgatat
     9961 tgaatgtctc atcccacgcc cagacagtga ctttttcttc tttgtctgct gtactactag
    10021 cacttccgca tcctgttaag aaacctgatg ctgccgctaa acaaacgact ccagcaatta
    10081 actttctgat gttcattttt atccgctcct tttccatcca ttagttgttt acgtttgcta
    10141 cattcatcat accaaaattt gaaaacggtt tctttattat tcagatactt caaaaatttc
    10201 tcctaaaata ataaaaaaat acaaatcaac tatgacaaaa ggaataatct tagcatctag
    10261 accaaagatc gaaatggtga ggtttcttta aaataaaaat atccttgcat tttctcctag
    10321 aaataagtat ttttaaatta caagactatt cttaaggcgg gtaaaaaaat gccaatacgc
    10381 gtaattctta tcgatgatga acagacgatt ttagatggaa tgaaacagtt gatcaattgg
    10441 tctgagcacg ggtttcaatt aatagggact ttttccagtc cgctgaaagc gcttgatttt
    10501 tctttggtac atgcaatcga tatcgtggtc acagatgtaa tgatgcctga attaaatgga
    10561 atcgaattat cgcggctatt aaaacagcaa caacctgaaa cacagatcct tatcctctct
    10621 agctatgatg aatttgatat ggttaaagat tcttttaaag aaggcgtcgc tgattatctt
    10681 ttaaagcctc gattgagtcc tgattttttt ctaaattcct taactgccgt ttcacaaaaa
    10741 atcaaaaaaa agcccaacaa aagcccaatc accaatcgtt atgagcagat ctgcgagaat
    10801 ctttcacagc agattgctgg acaaactcgt ccattgcttg accctcttct ttttgaacac
    10861 accttgtttt tctgttatat accgaaaaac ggccattcag ttcaaaacaa ggctctaaac
    10921 aaaaacagtc gatcacccat tcattgatgg atagcgacga ttcgtttact atttatcctg
    10981 tttctacctt ttcggaagaa tccggctatg taatcaatac tgattccaaa gatacactta
    11041 ggaagtttgt tcaaaagttt cgtcaagaag tttttccagg tgattttttt gttgtttctg
    11101 attcttttgc aatccaagat atcaatgaaa tattccaaga gatcagaaat tgcaccaaag
    11161 gacaacgttt ctatcataag ggaaagaacc ttgcttatca gcatgaactt cttccgttat
    11221 taaccaacca cgagcagcaa gcaagtagtt atttaacaaa aatcgttcat aaagactacg
    11281 ctttagctgt caaagaactt gagaatttct tcacgacgat attggaacag cctattgatc
    11341 cagcttattt gaaacatgag gcaatcaatc gtttctatgc attactcaac gctttggctg
    11401 acgagcatgt tgggaatgaa gaactttctt tgctcaagat cgcccttcct tctttattag
    11461 ccgatgctga gtatttcgaa gatttttcta ttcttgtcaa agaaacactt gagaaactca
    11521 cccacttttc tgcaaatgca aaaaaagaca atttcgatat cttgactttg atctacgagt
    11581 tcgtccagga aaattatgag aaagaaatca atttacaact tttatccgaa aaatatcatt
    11641 tttcatatag ctatctctca gccatcttta ctgaaaaatt tggtatcaac ttttcaaaat
    11701 atctaaaaaa agtccgtatc agtaaagcca agctcttttt gacggaaaca cggcttactt
    11761 tgacggaaat ctgtgagaaa gtcggctata ccgaactggg ttatttctcc agagttttta
    11821 aagaagagac aggaatgaca ccttctcaat accgtaaagg aacttttgta aaatgagaaa
    11881 aaaaatcaaa gagcatgaac tctttgccaa acttctgttc attttgttca tcggtctttt
    11941 tctgcaagcc atcgtcattt ctttgtttat ttatcatcga tcaagggatg cctatatcca
    12001 gctgttcaat caatcgaatg atgtggtact aaaaaagatt caatctgaat ttgaaagttt
    12061 gaatgataca atcgagaata cattagccgc ttttgattct aatccagctg tcaaaagcta
    12121 tttttccaat gatccagccc aacatatgga acagcttcag caactaagaa caatccaaaa
    12181 aatgaatgac tctctttcaa aaatccatcc aatgatcgat tacgatgtat tgatctttgg
    12241 agaaaatgga agaacttttg tcggaaacga catgctcact gccgtatctg ccgattcttt
    12301 ttttcaatct gcgattgctc agagagtgaa tgaacgtgct gctgatacac aaatgctttt
    12361 tgcacaccac ggattgactc ttcgcgataa gaaagcaccc agcgtcttct ttgtcaaaaa
    12421 actgaaaaat acattgaatc acgtttacgg atatgctgtt ttatcgatct catccaagca
    12481 actagcgaat ctttttcaat ctgtagtaaa tcctgagata tcgaagatct ccttcatgaa
    12541 cgaagaacag acgatcatcg catcaaatga agaaaataca attgggagcc gatcaatgat
    12601 tttcaaaaaa ctaacagtag gtgaaacaac gatttcaaat ggacagcagt tgacccgtct
    12661 tcctctgtac cgccaggatt gtacattact cagtcaagtc gatcttcatt ctttatctaa
    12721 ccaaatgggc gtgatttttc cgattatttt gtataatatc gtgacacttg cttttgctgg
    12781 ctcattggta tttatgtatt tggatcgaca cacaaaatcg atctaccgct tgattgattc
    12841 gctaaaacgg ctagaggaag atggcttaaa agctactgtt cctgtacaag gaagccatga
    12901 agtccaattg ctagggaata cgatcaatca attgctaagt aatgctcagc atcattatga
    12961 acagacgatc caagacgaaa agaagaaacg ttcgcttgaa atccaaacta tgcaagcaca
    13021 gatacagccc cattttattt ataatacgct gactagcttg aaatttttga tttggcagaa
    13081 aaaaacagat gaagcagttc aaggaatcga cagtttcatt actttactaa gaagtacgat
    13141 tgggaaaaaa gaagaagtga ttccagtcaa ggaagaaatc aaaagcgtac aaagttacat
    13201 cgatcttttg agtatgcgat atggcgaaag aatctcggct aaaataatga ttcctgatga
    13261 actgcagtca ttatctatgc cgaatatgat ccttcaacca attatcgaaa acgcctatct
    13321 tcatgctttt cacaataaaa cgcaaggatt catcacggtg tacggcaaaa caaaaaatca
    13381 gcttctttta ttcgaagtga ttgataacgg cgatggcttc gatactgaaa ataccaaaca
    13441 aaaaaatgat catttttcag gaatcggcat cagccatgta gatgagcgga tacgcctaat
    13501 gtatggcgat gcatatggct taacgatcca atcagtgatt gggacaggaa cgactgtaac
    13561 gatttgtcta ccgcttcttc ctaaaaatca atcagaatat caaaaataaa gaatagccaa
    13621 gcgctgattt ttttatttta agttaaataa atcaatcagt attcttgact ttcatctacg
    13681 cgtctcttct actgtcagca ctggataaca gcccaatatt tgactttctc ctccgagcat
    13741 cttgatttct tggagcgcat tctctactaa tgtcattgga cggtccaaca acaaatcgat
    13801 cacgaaaaaa tattctccca agctagtttt caatggtcgg gattcaatct tacttaagtt
    13861 gatttcccgc cagccaaaag ctgccagcat tttatgaagc atccctggcc gattagcagg
    13921 tagtgtcaag atgacactca tttttactgg agctgactgc tggctggtca tctttcggtc
    13981 accgacgatc caaaaacgtg tctggttcaa ctcattatct tggatattct cagctaagat
    14041 ctccaatcca acatgctgtg ctgtttctaa tgaagcaatc gctgctgcgt cttctttcgg
    14101 atgttctgct acatacattg ctgcagcagt tgttgattct gttgctacta aaggaacatt
    14161 aggataatgc gtttctaaaa actgttgtga ttgtgctagt gcttgtggat gtgaaaggat
    14221 ttttgtaatc tttgttgctg gattgcctaa aagctgctgc ttgataggaa gaacgatttc
    14281 acttttaact tcgacttctg gatgtttgga aagaagatcg attgtgtgat gaacagctcc
    14341 ttccaatgaa ttctctacag gaacgacagc cagatcgact tgtttgcgaa acaaagcgcg
    14401 caaacaaaca gggatggaag catacgcagt caattgttct gtaggaaata gctgacaagc
    14461 cgcctgataa gtgaatgaac tttctggccc tagatagctg acgttcatat gcttttgatc
    14521 ctttctataa tggtatcgat gatcttttct ggtgtttgat tggtcgtgtt gattcgatgt
    14581 gtcgcacttt cttcatacca agaaagtcgt tgtgccaata atgcttttat ttcttctctt
    14641 gtttttccaa gagctaaagg tcgaatagac gtttcatctt gttggatacg atcaactaga
    14701 cagtccgctt ctgcctctaa gtaaagaaca atcggctgtg tttttaaaaa acgccgattt
    14761 tctgcacttt ggacaatccc tccaccagtc gcgataattt tgtcttcatt tgacagcttt
    14821 cttaaaatat ctgactccac ttttcgaaag cttttttcac cgtaatagtg aaaatattcg
    14881 gcaatcgaca attggatatg ttcctcaatc tttgtatcta aatctacata ctctttttgt
    14941 aaacattgag ctaacccttt accgattgtt gtttttccag cgcccatgaa accaatcagc
    15001 aaaatgctag ccatgagctt ctcctcctga cacaagttct gctaaatcgt caaaaaatgc
    15061 cggataagag attgaaacag cttcagctcc ttctagttcg caaggtccat cagctatcaa
    15121 agcagctact tgcagcatca taccgattcg atgatcgcct ctgctggaca cttttgcgcc
    15181 atgcagaggt gtcggcccat ggatgatcag accgtctgct gtcgctttta tctttgctcc
    15241 cattttctgt aattcttctg ccaccgcatc aattctgttg gtttctttta cttttaattc
    15301 ttctgcgtct ttgatgatcg tgattccttc tgcttgagta gccactaaag ctagaatggg
    15361 caattcatca atcagacgcg gaatgatttc tccaccaatc gttgctttct tcaaatgact
    15421 cgtcttgaca cttaaatcag ccgattggtt ctgttcattg tgattcgtct gggtaatcgc
    15481 tgcgcccatc tcctccaaca catctaagat accagttctt gttggattga ccccgacatt
    15541 ttttaacaac agttggcttt ccggaacaag aagtcctgct gctagaaaga aagcagctga
    15601 tgaaatatca cctggaacag ttatctgttg accagttaat ttttgcggtc cggtcactat
    15661 gatcgtttta tcttccactg tgatccttcc gccaaattga cgaatcattt cttccgtatg
    15721 gttacgggaa cgttcttttt caacgattac ggatgtgcct tctgcttgta atgccgcaaa
    15781 cagcaaagca gatttgactt gcgcactggc tacaggcata ttgtatcgga taggtgataa
    15841 agagcgggtt cctgtcacag tgattggtag atactggtca ttttcttctc cttgcaagtc
    15901 agcgcccatc tcgcgtaaag gttccatcac tcgccccatt ggacgtttag acaacgaagc
    15961 atccccgaac agagtagtcg taaaaggctg tccagccaaa atacccatca ataaacgtat
    16021 ggtcgtaccg gagtttccca tgtcaattga ttcggttggt tggacaaatg agtggctacc
    16081 gcgaccatga atcttgatca cctcttcctc ttcttcgatc tcgacaccta attgttggaa
    16141 ggcatgtaaa gtactcagac aatcttctgc gcgcaaaaaa ttttgtatag tcgtcgttcc
    16201 ttcagcaatt gctccaaaca tgatgctgcg atgggaaatt gatttatctg ctggtatcct
    16261 aacagtccct cttaatccat gtatttgttg taataattgc atttatccag cctcctattt
    16321 gatccagcaa tgataactgg tattagacat aatgattctt tttgcttgta acaaatcctg
    16381 ttggttttta aaactgattt gcagtattcc taaaatatct tccctggtct cttggatctt
    16441 caaattaatc aaagaaactc ccgcttttcc gatcagtcca gttacttctg caataacgcc
    16501 aggttcatct ggcacatcga taaataaatc aaaaaaggca ggaatcgcgc cttgcttatg
    16561 gacaggcaac tggtctcgac tttcttttgc ccgattgaaa aaagaaaaga tttccgattg
    16621 gttctcagtc atgatccatt ctgctatttg tgtcatttct tgttgccaat ctgccaatcg
    16681 ttccaataat atctgcttat tgctcaacaa aatatctgtc cacatgacag gatctgagga
    16741 agcgatccta gtgatatctc gaaagcctcc tgctgccagt tgtttggctc gcggatactc
    16801 ttgattgaat tgttctgcct gattgactaa gccagctgcc agaatatgtg gaaaatgact
    16861 tagcattcct gttatacggt catgttctct tgctgtaaga gttacatatt ttgctttggt
    16921 tccgcaaaat agttcttgca gtttttcaac atcttttttc gtcttttcca aaggtgtaaa
    16981 gatgtaatag gcattttcaa atagtttagc atctgctgcc tgtatacctg atttatgaga
    17041 accagccatt ggatgtccac cgacaaaccg aagatttttc tgctcagcgg atgtcacgac
    17101 ttgctgcttc gtactgctga catccgtaat gatgacgtga tcagacaagt tgattctttc
    17161 aaggacatca agataagcta aggttgtacg gacgggaacg gctaataaga taacgtctgc
    17221 ttgttctgca cctgtttcaa aatcacttgc aacgtaatca acgatctgct gttctactgc
    17281 ttgctcacgg gtttttgact gactatccca accaactact atcagttctg gattggtctg
    17341 acgaatacaa agtgccatag aagcaccaat cagtccaagc cccacgataa aaacatgttg
    17401 tgccatctta tttgccccct ttttagtaat gtttcaagta gtcacggtac tccttcactg
    17461 cagctttcaa ctcaacaagc gaatcacttg ggaattttga caatagctct gtcgctacca
    17521 cagtggctac aacattttcc gcaacgacgc tagctgcagg aactgctgta ctatccgaac
    17581 gttctacact tgctttgtaa ggttcttttg tatcgatatc aacactttga agtggcttgt
    17641 acaatgtagg aatgggtttc atgaccccgc gaatgacaag tggttcgcca ttggtcattc
    17701 cgccttcgaa gccgcctaga ttattggtac gtcggaaata gcctctttct tcttcccata
    17761 ggatctcatc catcacttct gagcctggta atgaaccaga agtgaatccg accccaaatt
    17821 ctacgccttt aaaggcattg atactaacca cagcttgtgc gattttggca tctagcttcg
    17881 tgtcccattg tacgtagctt cctaatccag ctggaacgcc tccaacgagc acttctacaa
    17941 ttcctccgat cgtatctcca tttttctttg tctgatcgat caaatctttt attctttgct
    18001 caactgctgg atcaacaact cgtacttctg acgcttcaga aatttctttg atttcttgaa
    18061 tcgagatatt tccaggtatt tctgcttgga ttccaccaag acaaatcacg tgtcctgcta
    18121 cgatgatgtc aagttcttcc agaattttct tggcaattgc gccgattgct acacgcatgg
    18181 tcgtctctct tgcagaggac cgttcaagca cattccgaag atctcgatga tggtatttca
    18241 taccgcctac caaatccgcg tgtcctggac gcggacgtgc aactcgccgc agtgattggt
    18301 ctttttcagg cacttctgct acagacatga cagttgtcca atttttccaa tctttatttt
    18361 ctaccactaa agtgacaggt gaaccaattg ttttgccgtg acggatgcca gacgtgatcc
    18421 gaacacgatc tttttcgatc ttcatccttc ctcctctgcc gtaaccagct tgtctgcgag
    18481 cgagttcgtg attgatttct tcttctgaca gaggtaatcc tgctggtagt ccttcaataa
    18541 tggctgttaa ttctggtcca tgggattctc ccgcagtcat ataacgcatc ttacagtcct
    18601 cctaagtaat ctttgaatgt ctctgtggga acaggaacga tttttgcttg cccaatcttt
    18661 tctagcaaga tgatattcaa ttggcttcct cttgctttct tatcatgggt tatcgcgtgg
    18721 aataattcat ctggatgact tagatcgtac gtaatcggca gatggaattt ttgaagtatc
    18781 ctttctaaag cagctgtcga tccagttgga gacaagccga ttttttctgc atgagaagtg
    18841 atggctacca ttccaatagc tactccttct ccatgactga ctaccccata tcctgctgta
    18901 ttttcaattg catggccaat tgtatgacca aaatttaaaa taagtcgatt tccttggtca
    18961 aagacatctt cttccactac ttttcgtttg atttccaacg tagcagtaat gattggaaca
    19021 gcatgttcca gaagatcttt ttcgtctttc agctcgttca gctgtttcca aagcgttgca
    19081 tctgctatag ctgctgattt gatgatttca gcgattcctt ctcgtaaacg acgtaccggc
    19141 aaagttttta gaacctcagg atcaatcaat acaccagctg gctggctaaa tgttccgaca
    19201 aggtttttcg ctttgtttgt attgactgct gtcttcccgc caatactaga atctacttgt
    19261 gcaagtagcg tagtcggaat ttgaacgaaa tggatcccac gcatgaacgt cgaggccacg
    19321 aatgctgcta gatcacccac cattcctcca ccaagtgcaa gtatcccatc gctgcgggta
    19381 aaatcattat ctgctaaaaa atcgtataac tcagctgctt gttctaaaga tttactggtc
    19441 tcacccgccg gtacgattga aaggactggt tcaaaattag cttgtctcaa ttggaacatg
    19501 attttttctg catatagtgg agcaacgttt gtatcagtga tcacagctac tttttgaggt
    19561 ctccaaatcg tttgaagcca tgatccagcc tgttctagtg cgcctttttc aatatgaata
    19621 tcgtattttt ggttatcaac atgtacagta agcattcgtt tcacttcttt cttttaaaaa
    19681 gctggaatta atcgagtgtc tgaatctttt ccattgcttc tttcatgatc gcaacttctt
    19741 tcatcatgcg tgagtaagtc ttttcattca acgattgggg accgtctgac caagcatttg
    19801 caggatcagg atggatctct acgatcatgc catctgctcc gcttgccaca cccgcacgag
    19861 ccattggtgg tacaagttcc caaattcctg taccatgact tggatcaaca atcacaggga
    19921 aatgagttaa ttttttcatc aatgggacag cacttaaatc aaacgtatta cgtgtcgctg
    19981 tttcgtaagt ccgaatccca cgttcgataa aaatgacagg agatttgtct tcgactgcaa
    20041 tgtattctgc agcattaagc cattcattga tcgtaccgga aattccacgt ttcaatccaa
    20101 caggtttacc tgttttaccg acagcagata gcagtttaaa attttgcata ttacgtgcgc
    20161 caatctgtag gatatcgctg tactcagcca ccatatcgat atgcccttca tccatcactt
    20221 cagtaatcac tttcattcca aattcatcag ccgcttgacg gatgtatttc aatccttctt
    20281 cttctaaacc ttggaaagca tatggagaag tacgtggttt gaaggcacca cctcgcagaa
    20341 tttttgcgcc cccggctttg gccattcgcg cacattctct gatttggtct aatccttcga
    20401 tagaacaagg accagccatc gtcacgaaac tgccgtcacc aatttttact ccgtctacat
    20461 ccacgactgt attgccagga tggaattctc gagaagtcaa tttgtatgtg tttaaaatac
    20521 gcacagcatt ttctactcct tcatagcgtg taaatgctcc ttcttgtaaa cttcgcgtat
    20581 caccttttag tccaatcact actcgttctg ttccttgatc gattgagact ttgaatcctt
    20641 ctttttcgat atgatcgacg acttttttta tttgttcttc cgttgcttct tttttcataa
    20701 ttactatcat tgtttttccc gctttctttt gtttacttat ctatcttggc tgattttctc
    20761 tactttttca aggtggtttt acattaattc ctactaaatg accgtgtcct gttgttctca
    20821 ttttcgctcc ttctttccta cttatgaccc agataatcaa caaaaaaaga tgtccctgta
    20881 ggaaattgcc tctacagtga acatcttcaa cgagccttaa aaagcgctgc aaaaatataa
    20941 aatagccact atagatgaat ttctctacgt ggctgtaagt cgtgtttaaa aatctttagc
    21001 catcatagat acaaacctgg tttgtttgta cccacgaagt catgaataca aacctatcta
    21061 aaatagctaa agtaatacgt attcagtttt gtaagttttc tagatttcat aaaaaatcgc
    21121 tccaaactga tgtaagaatt gtacctagta tagacataaa aaaatcgact gtcaatcatt
    21181 tttatgaaac tttttttaat tttctgatag ttaactttgt ttttcagaaa aataacaatt
    21241 ggttttattg aagaaaatag tcatgaacaa aagtatctga gaactttttt actctcttgt
    21301 tcatgacata tagaataaag cagctttgtc ttgtaaactc cgtttttcaa ataataatct
    21361 aaagattgct actgaataat aaagccgatt tcatggtcct cttcgctgct gattcggtaa
    21421 ggtgactcaa catccgctcc ccagctattg atacccccga ctcctcttac ttttgcaaaa
    21481 atcgtaagaa ccgttcgtct ttctggcggt agttcttcta tatgtgttgc ctgttccagt
    21541 tcttctgctt tgaatggtaa agcggaaaaa gcaaatggcg tatcctgctg ttcaaatcgc
    21601 aacgaaaaat cttcttgatc tttatccgta ttatccaacg tttgatttct tgttagttct
    21661 acccatttcg tttccatatg catgccgcat tcttggggaa caaggtatgg ggaaacgggg
    21721 agtcctgcca ctttataaat accttcgaca ccaccagcca tccgatccgg atatgtttcc
    21781 cctgataggc cttcatactc aaaaaatttt gctttcgttg gcataatgaa ccttaaaccc
    21841 aatgcgggta attccggtaa tcctttttgt ccataataat gcatattgac aagcatttta
    21901 ccctctccag cgatttggta ggaaattgtc acttgggttt ttggtactgt gttgatttcc
    21961 agtgtaaaaa tgatttcaac cgtttcagcg tattcattac ttgaatattg attatttact
    22021 ggagcaatcg gcaaatcgat ctttttattg tccaccagta cttctatttc tttgactttt
    22081 ggatacaatc cggctcctaa ccacataccg gatttaaatc caaatccgct acctctgtca
    22141 ttatcggtcg ttgctcgcca aaaaactgga agtgtttccc ggtatagcca ttcttttccg
    22201 tttttattga gcgattccag tccgcctctt gtataagaaa aaatataatg cgtattcccc
    22261 gatttgacac ctaatgtaca gtcgccgaag acgattgcaa ctttttccgt atccatatga
    22321 cttttattta ctgtattttt cataataata tcttacctcc tgcatggcgg gtttttcttg
    22381 tccatctgca aaaacgatcc cattccctga gaattcatag tcacttggtt tatcgtcaaa
    22441 gtcaccgcca tagcgcatca cttctctgcc tgtgattgga tcagtcactt ggatcgcctg
    22501 atctttaaaa tcccatatat agcctccttg gtacatttca taacgatcaa gcaacgtcat
    22561 gtaagaatcc attccaccta acgaattccc catgtcatgc atatattcac ataagatgaa
    22621 cggtttttcc gctttatttt caaggtagtc aatgatctct tctggtggcg catacatgca
    22681 gctttttaca tctgaaatcg aatcttcgaa tgctggattt ggaaaattcc cttcataatg
    22741 gacaagtcga tcggggtcta cttttttgaa gtaatcgttc atttgtcgaa tgttttcttc
    22801 cgcataagat tcatttccaa gagaccaaaa aagaatactt ggatggtttt ttaatgtttc
    22861 gaaattcgtt tttgctcgat caagaacagc cgcctgccac tgaggtactg cacctggtac
    22921 gttgtaagaa ggctcaaatg cgcccatttt ttgccatgag ccatgggatt cgaggttcgt
    22981 ttccgccatc acatggattc ctgcttggtc gcataaataa taccaagtga tctggttggg
    23041 gtaatgagat gtccgtacgc cgttgatctt tgcatttttt atgatagcca aatctttttc
    23101 catatcttct atagtgatgg cgcgtccttt tttcggatgc cactcatgtc gatttacccc
    23161 gttgataatc atccgttttc cattgaagta gatgatttta tctttgattt cgattttacg
    23221 aaaaccgaag gaatagtcaa tatactccgt tactcttttt tcttcgtcta tcgtctccaa
    23281 ttcaagctta tataaattgg gctgaaaatg cccccacagt gccacatttt caaatccatc
    23341 gatgcgaatc gtcatttgct cttttaacgg atattgctgt tgatagattt ccttcccatc
    23401 aggatctagt atcgctacat tcagctgact gtttttggct acttgctcaa aagacaaatc
    23461 tacttcaagt ttgccatttt gacatttgtc atctaagcga ggtttgatcc aaagatcttc
    23521 taaatggttt tgtcctcgag caactaagaa gacatctctg aagatcccaa aaaatcggaa
    23581 aaagtcctga tcttccaaga atgcagctgt acttcttttg taaacctcca cagctagaat
    23641 attttcttct tccaaataag gtgttaaatc aaattcagac ggagtgaacg aatcttcggc
    23701 atacccgata aaatgaccat tcaaccaaat aaacattgct tgttctacac cagcaaaatg
    23761 aatcgagatt tgttttccct ttagaccttc ttctaatcga aaagtcgtca aatacgaacc
    23821 aaccggattg tctttagcag ctgaaaagat cccttccgcc tctttctcag ggcttgtcgt
    23881 gaaaggggga cggcgaaata catgtccctc ccacggatac atcgtattga tgtaatggat
    23941 cttgtcataa tttgctaatt caatatgact aggaacagtg atttcatcaa aagaacaggc
    24001 atcaaaatct tttttataaa atgtttttgg tcgtaccgaa ggatttggcg aataacaaaa
    24061 acgccatgat ccatttaaag actgtttaag ggaggatgtg gtttctactt cttcccttgt
    24121 tgcataacaa acatgatcgc tatgtgcatt tgattggttc actctaaaaa cttgtggatc
    24181 atctaaccat tttatatttg ctttcatact tttttccttc tctctcataa ctttttgaaa
    24241 tgaagcagtt ctgtttggaa atcaccttga aaatcgggga agtaaagtcc aactgtcatc
    24301 aattcggccc ccgaaaatat ccgcttcgag gtctgttcct gataaagtgc ctcatcatct
    24361 aaccccatca gatatacttc atggaacgct ggttgagctg aagctagtat cctccctaaa
    24421 aacacaatag cttcatcttg tttaggagac acaaacatcc atgcggcaat attttcttca
    24481 aaaggactcg ccaaacgata ataatggcca tactggatca ccggacgaat cgtttgatag
    24541 gatataattt ggtcttttac ttgctttttt tcttcaggag acagctttgt caaatccagt
    24601 tcataaccaa aaacagcgct catagctgtt tctcctcttg ttttcaacga agtagatctg
    24661 tcggtctgat gatttggtac agctgataca tgagctgtga tggacgaaat cggataagca
    24721 aggcttgttc catattggat tttcatacgc tccactgcat ccgtgttatc acttgtccat
    24781 gactgcggca tataataaat aaacccggca tcaaaccgtc cacctccacc agaacagcct
    24841 tcccataaga tttccggata atcagttgtc aaatgttcaa gtagttcata aagcccgagc
    24901 atataacgat gcgctacttc tccttgtctc tgtgggtcaa gggtaatcga atagacatct
    24961 gaaagcgaac ggttcatatc ccatttgatg tagtcaagag gaatcgtatc taaaatcttt
    25021 ctcatttgtt tctcaatcgt ctgccgaaca tctaaccttg taaaatccag tacatgttgt
    25081 gatctggaag cggaaggcat tctacctggc tcctgcataa gaaaatcagg atgtgttcga
    25141 tacagttcag aatcaaccga gatcatttca ggttcaaacc ataaaccaaa tttcaatcct
    25201 ttgctatgag cataatctgc tatttcacgt aatccatttg tcaacttgcc ttcatactcg
    25261 aaccagtctc ctaatgaaga attgtccgca tctctttttc caaaccagcc atcatctaat
    25321 acgaacattt cgatgcctag ctcctttgct tcatcaatga tcgccttgat tttttcacta
    25381 ttgaagtcaa agtaagtggc ttcccagtta ttgaccagaa tcggccgttc agcaaattga
    25441 tgacgacctc ttgcgattct ttcacgtagc aactcatggt ggatctgact cattttgttc
    25501 aatccctgac ttgagtaaga aaggatcatt tcaggtgttt gaaaagactg gcctgcattc
    25561 aattcccacg aaaaacgatg gctgttgatc ccaccgacga ctctcaattg attgatctga
    25621 tctttttcaa gttcgaaaga atggttgccg gaataaacaa attgaagtcc gatcgcttct
    25681 ccagtatttt ctgtcgtatg atgatgaact aaagcaataa agttattcat atggtgactg
    25741 ctcgttcctc gacggctttc aaatactttt gttccagata aaacagattg ccgtgaaatc
    25801 tgtctttcat tgacatgtgc tcctggcaaa gcaatgactt catcaaatcg cctagtatgg
    25861 gcaaagtcta gctggaaact agccgctttt tctagtttga ttgtttctcc ggtttcattt
    25921 cttatcttta cagatcgtgt gatcactgct ctatcagtaa atatggtata gaacaaatcg
    25981 aaataaatcg ctgtttctct atccttaagt gaaatcacca acgtttgtgc ctctgacttg
    26041 tccatcacat aagtttgagg caagccttcg atctctggct tcccatctgt aatataatgg
    26101 ctgtcatatc tcagatccag taagtctgat ccattcttgc gctgaatgat cgatgcgggc
    26161 gttcgaaagt ccatgctgcc atgagaagag tattcttgcg gtagtgtatc tttcgacaga
    26221 gaccggtcag gatttaaggg aacatttcct gaaaatccac gatctcttcg gggataattt
    26281 ttattgtctt tgtactgctt gactggtttg ccaaaataaa tatgcgccaa tacattgctt
    26341 tcttctatct ttatcagata agaaagaaat gtatttcgta aatggaagac tcggctcttt
    26401 tcatcaaata agatgaaatt tttatctgtt tttctatgcg taatatccat catttcactc
    26461 ccaacaaaac ggaaaccgtt tacttttttt ataattcctg tatactgttt actaaacaat
    26521 tttgtcaata aaaatttttt atttttttga ggagtgtgta aaaatggttg gaattagaga
    26581 tgtcgcacga aaggcaaatg tctctcctgc tacagtttcc cgtgtgttga ataacgatca
    26641 gacgatccat gtggctcaga ctactcggca acggatagta gaggcagctg ctgcgttgga
    26701 ttatgacatc agtactcgta aatatatcaa aaagagaatg ccttcgattg gtgtgatctc
    26761 tacgatcggc aaagcgagcg aagaacagga tatctattac aaacatttgc gagtagggat
    26821 ggaagaagaa gctcgacgtc tgcatttagg gatgaatcga atctataatc tatctgataa
    26881 tcctaaacaa tgggaagatc tagatcagtt aggtgcggtg atcgttgttg ggactgttac
    26941 gaaagaatcg attaccgcct tgttaaaaca aaacatgcaa atagtcgtcg tggataaccc
    27001 tgacatccag caagaggtcg atatggtgta tgcagatctt gaacggatga ctaaaaccgt
    27061 actggaattg ttcttagaaa atggtcataa aagaatagcc tatatcgggg gatataaagt
    27121 cgatatggat gaattcggtg ggaagcagat ttctataaat gaaaagagga tgcgtgccta
    27181 tcagcatttt atgatcgatc atcacttaga aaaatatatg tcttatcatc taggtggctg
    27241 gacagaaaaa caaggtgagg tacttgcgac taatctatta gaaaatgaat cccctcttcc
    27301 tactgccatt cttgtcggga gcgatccaat ggcaattggt gtctatcggg cattgaaaca
    27361 aaaaaatatc caaattggag aagagattgt tgtagcaagc tttgataata tcgagacaat
    27421 cgcaaacctc tcccctggct tgaccagtgt tcaaatcaat gcgaaatcaa ttgggcaagc
    27481 agctgttcga atggcattgg agcgaattga cggtatacgg gacgaaccaa tcattttgac
    27541 ttatcctgca cgattgattg ttagggaaag ttttatccca agaacaaaaa ggtattgaaa
    27601 gagaggttgt gaaaagccgc ttcgtttctt catactcaaa acgaaacggc tttttcacaa
    27661 cctcttactt atacttattc acaaaatcca atgtctgctg gtaacaattt tgcgattctt
    27721 ttagcgtgta atcaaattgg tattcatggg taatctcttt ttttgtatct gtataaaaca
    27781 aggattgcac gggtaccttt aattctgtca accgattttc taatgcttga ccttgctcct
    27841 ggaatgagta tgcatttccg tcagttatat acgttggcgg aaattcctta gaaacatggt
    27901 cagccacact agcttctttc aattgctcac tcgttttcca atcttttgtt ccaatcaacg
    27961 accacgcaac ggttttgacg aaaaacttca taaatcgatc atccgaatgc tgctgtgctg
    28021 ttagcttcaa atcaacaggt ccgcaataag aaatggttcc tttcagacta tcttttggaa
    28081 gtagctgttt gattttcatt tgtttagcat acgcatcatt cgtttgtata gctgcatatt
    28141 gcaatgcgat ttgtgaacct gcactatctc caccaaaaaa taattgttcc atgtctagca
    28201 atttgtcgtc tttttcttca gctaataaat ctttgatcag ttcattcact tgaagaactt
    28261 gattagggta ttctgaatca ggcgctaatt cataattcat cgcaaccact gcgatgttgg
    28321 cgtctgaaac taattttgtc gcaaattctt tgacacccga tttatctccg ccgacgaatc
    28381 ctccaccatg tacccaaaat agaacaggaa caggcccctt gctgtttttt ggatagtaga
    28441 tgtcataagt attctcttta tacttggact catacgtcaa atctgttttt ttcgccacgt
    28501 ttttttctgc ttgatcgaac gtttttttat cagtgatttg aacagtattt gaaaacatat
    28561 ggttgatcaa gaatgctcca ggtctggggg aaagctggaa ggcaagaaag attgcaaacg
    28621 cgatcccaag aatgattccg aagaaccatc cgataaattt aagaattttc tttttcataa
    28681 ggctccctca gtagttagta ttaagcattc tgattatacc atagtgttat ttcctatcaa
    28741 gctctcctag atgatttaat gattttcccc aaacttcttc tgcaatgctt ttgacaaaag
    28801 aaattttttc cattgttctt caaacaaaac attttcttct tctgttgaag caaatccaca
    28861 ttgaggactt aagcacaaac gatccaaagg cagatatcgt gtcgcttcgt agatccggtc
    28921 aatcacttgc tggtgctctt ccatttcgcc atttttcgtt acagtcaaaa tctaagacaa
    28981 ctaatttatc ttcagagact ttctctaaaa gaacaaaact cccagcacgt tcactatcgt
    29041 attctaaata ataggcacgg acattttctt gagtaaaata atgggtctgc tacgtcgtca
    29101 tcccccctga tgccgccaaa atcgaataat aatttccgcg tcaaacatgt atatttagtg
    29161 ttgaatcatt tgagaatatg aaaataaaaa tctttttgat caatcagtta atcaaattga
    29221 aataacgaaa aatctgagac agaatgacgt tcattctgac ccagatttga ttttgcacaa
    29281 ctcaacagtc cttattggca aatcgccaaa ataatgtaga ctaactaaaa agactaagaa
    29341 acaataacaa aagttaacac ttcttagtct ttctttagca cgtctattta ttaaagattt
    29401 tagggccttt tctcatgttt ttacgatcat tcttaggtgt ttccatttgt ttggatcggc
    29461 ctccaccttg tttctttttg atcaattctt tcattttttc tttttggtcc atttttattc
    29521 tcctttaacg aatgaagcga ctaattcgat atgatgggtt tgcgggaaaa gatctacagg
    29581 ctgaactttc ttcacggcat aacctgcttc tgcaaataac ttagcatctc ttgcaaaagt
    29641 tgccggatta catgagatat agacgattcg ttctggtgac atttcgattg ctgattcaat
    29701 aaaatgatga tctaatcctt ttcgtggagg gtcaactacg atcacagatg gtttgatccc
    29761 tttttcttgc cattttttca taactttttc tgcttttccg acttcataat gaacattttc
    29821 gattttatta tttaaagcat tgaactgtgc atcttcaatt gcttctggaa taacttccat
    29881 cccatacacc ttatccactc tgtctgctaa agacaagcca atcgtaccta ttcctgaata
    29941 agcgtcgatc acaatgtctt ttttatctaa ctcggcaaac tcgatggctg tttggtacag
    30001 atactctgct tgttgagtat ttacttgata aaatgacttt gctgagattc gataggtttt
    30061 tcctaatagc tgatcttcga tatagtctct gccatataaa actttttctt tgtctcctaa
    30121 aatcacattt gttttttctt cattgatatt ttggatgacc gaaaccactt ctggcaattc
    30181 ttcatgaata gcttcagcaa tttccttgga tttaaagaat ttttctttac gagtaactaa
    30241 aacgaccatc atttcatggg aataatgacc acgacggata atgatgtgac gaagctgtcc
    30301 ttcatttttt tcttcattat atcctcgtac ttggaaccgt tgcaagatat ctctgactac
    30361 tttgattgct tgatcgatga caggatcttg gatataaaaa tcttcgatag ggactagatc
    30421 atggctattc tttttgtaaa aaccggtggc taactgtcca tcgatacggc gaacagggat
    30481 ctgtgcttta ttacggtagc cgaatggttc tgccattccg accgttggaa gtacaggaac
    30541 ttctggcatc ttagcaatct tcgccattac atgctctact tgtttttgtt tgaagacaag
    30601 ctgttgttca tacttcaaat gggctaaagg tgcgatacct atgcgaagta atgcattgtt
    30661 ttctacaggc tgacgatcag gtgattgtgt aataaatgtt tcgacttttc catatccaaa
    30721 cttattgcct gcttttacta ctagtatttc tacttcttct ccagggagtg cattttcgat
    30781 aaataatgga taaccatcga tttttgctac ccccattcct tcataactca agtcgacgat
    30841 ttcgactgta tatcgttcgt ttttctttac tatttgctgt tctgtcatat tttgctcctt
    30901 taagcctatt tttcaatcct actgaactct actataaatc agctaattac acaactaaaa
    30961 ccggcactga ccttactacg ttcagcaccg gcttttgatt aattcttttg attaggtgtt
    31021 tcgtctgcta tttttccgtc accgtcgata tcttcatccg ttaaacgttc gacttctttt
    31081 acgaattctt tgctgacaac ctctaattct tcttcatctg tacctgtgat tgcttcatca
    31141 ggaatctgat cggtattggc aaagaactca atgtgttgat gaagattttg gaaatgcatc
    31201 ggtgcttccc cgccgtattc accatctaag ttgatcatta ctggtcgatt gatatcatct
    31261 tctgtttcaa cgaacaaact gcttgttttg gtatagatta ttcttggatc atcgacatgt
    31321 cgaccgccat tcagcatgag tgctgcaata tgcaagatct caaagatatt tgctgtcttc
    31381 acaatgatta aagaaaactt cccatcatcc aatttggcat ctggcgcgat ctgttcgaat
    31441 cctccaacag agttcgtcag ccctaggaaa aacatcgaag catttccgat atattcccct
    31501 tcatcatact tcaagcgcat tttgattggc tttactcgag ggagcatctc tgctcctttc
    31561 gcaagataag caagataacc aaaaatactc tttagttctg atggaacttc ataagtcaat
    31621 tctgtcaaat agccacctgc agcgatattg atgaagtaac tgttatcaga aaggccgatg
    31681 tccattttta cggtttggtg tttttttatc acttctgctg ctgctttaat attatctcta
    31741 ggaattttta atgctcgagc atagtcgttc gttgtaccag ctgggatgat ggccatttta
    31801 ggacggtgtt caagcggagc gattccatta accacttcgt tgattgtgcc atcgccacca
    31861 gctgccacta ctagatcaaa acctagttct gcgactctac atgcttcatt cttggctgaa
    31921 tctggttctg gtgtagttgc ataggcgctg gcttcaaatc ctgcttcttc taatgcctgt
    31981 aggatatccg ccaaattttt tttcaataat tcttttcccg aggtggggtt ataaattacg
    32041 cgtgcttttt tcattttcag cctctatcgt ttcgctaatt catctttaag tagcttattc
    32101 acgacaccag gatttgcttg tcctttagtt gctttcatga tttgaccgac taggaagcca
    32161 accgcacgat cttttccatt tttgaaatca tcgacagatt gttggttgtt atccaaaatt
    32221 tcattaatga ttggcagtag tttgttcgga tcagataatt gcacccaacc atttttttca
    32281 actacttctt gggcttctcc gccatttgtg atcaattcac ggaagacttt tttggcgatt
    32341 tttgagctga tcgttccatc ggcgatcaaa cgaatcattc ctgctaagtt ctcaggtgtc
    32401 aattttgtct cagctaattc cagtttctca ctgttcaagt aagcagaaac ttcacccatc
    32461 aaccagttag atgcttgctt ggcatctgca ccattttcca aaacagcttc aaaaaaatca
    32521 gacatttctt ttgtaagtgt caataccatt gcgtcatact ctggaagtcc aaactcacgg
    32581 atatagcgtt cgcgacgaga agctggcatc tcaggcaaac tagcttttac tttttgaatc
    32641 cattcgtcat cgatcacgaa tttcgggata tcaggctctg ggaagtaacg ataatcacta
    32701 gacccttctt tgacacgcat caaaactgtt ttattcgttg tttcgtcaaa acggcgtgtt
    32761 tcttgttgga tttctcctcc tgaaagaaga acttttgctt gacgtttttc ttcaaaagct
    32821 aaaccttttt tcacgaaaga taaagagttc aagtttttca attctgcttt cgtaccgaac
    32881 tcttcttgtc cataagggcg aagagaaata ttggcatcac agcgcataga gccttcttcc
    32941 attttcacat cgctgacttc tgtaaattgg ataattgatc gcaatgcttc tagataagcg
    33001 tatgcttctt ctggtgaacg catatcagct tcagaaacga tttcgatcaa aggcgtgcct
    33061 tgtcggttca aatcaacgta agaataaccg ccaataccat gcatgttttt tccagcatct
    33121 tcttctaaat ggacacgttc gatccggatt tttttctttt taccttctac ttcgatttcg
    33181 atccatccgt catgaccgat tggttgatca aattgggaaa tttgatatgc ttttggatta
    33241 tctgggtaaa agtaattttt ccggtcaaaa tgagtatctt tagagatttc acagtttaat
    33301 gccaaagctg ctttcatccc gaattccagc gcagctttat tcattactgg cagcacgcct
    33361 ggataacccc agtcgatcac gtttgtattg ctgtttggtt ctgcaccaaa atgagctggc
    33421 gctggagaga aaatttttga gttggttttt aactcgacgt ggacttctag tccaatgact
    33481 gtttcaaaat tcattcgtct ttccctccta aaatagctgg ttgttgttta tggaattctg
    33541 ttgcttgttc aaacgcatat gccgcttgat acatcgtttt ttcatcaaaa taattaccga
    33601 tgatctgcaa tccaactggt aatccttctg ataaacctgc cggtacagac atacctggaa
    33661 gtccagccaa gttgacaggg atcgttaaga tgtcactcat gtacattgtg attggatcgt
    33721 taatgttctc accgattccg aatgcaactg ttggtgaaga tggcccgatg atcaagtcat
    33781 aatctgcaaa gactttttca aaatctcgtt tgatcaatgt acggacttgt cctgcttttt
    33841 tgaagtaagc gtcataataa cccgcgctta aggagaatgt acctaacatg atacggcgtt
    33901 ttacttcttc accgaatcct tctgaacgtg agttgacata tacatcatct aatgatttca
    33961 cattttcaga gcgataaccg tagcggatac catcgaatcg ttgcaaattg gaacttgctt
    34021 ctgatgaggc aatgatataa taaacagcca caccgtattt tgaatgaggc aagctgactt
    34081 cttccacaac agcacctaat gaacggaatg tttctgctgc tttcaataca gcttctttta
    34141 cgccaggggc cacaccttcg cctaagtatt ctttaggtaa agcaatcttc attcctttga
    34201 tgtcgcctgt cagtccctct gcaaaatcag gaactgaaac gcctgaagaa gtaccgtctt
    34261 tttcgtcata accgctgata gctgtcaatg ctaatgcatt atctttcacc gtacgggtca
    34321 ttggtccgat ttggtcaaga gatgaagcaa aggcaatcaa accaaaacgt gagacacggc
    34381 cataagtagg tttcatccct acgatgccgt taaaagctgc tggttggcgg atacttcccc
    34441 cagtatcact acctaaagaa actggaattt gtccagctgc aactgctgct gcagaaccac
    34501 cagaagagcc accaggtact tttgttgggt cccaagcatt ttttgttttc ttgaaataag
    34561 aagtttctgt acttccaccc atagcaaatt catccatgtt tagcttacca acagggatca
    34621 tatctgcttg atagatttta tccatgaccg ttgcatcata aattggttca aaattgtaaa
    34681 ggatcttaga tgcagcggtt gtcaagatat cttttgttac gatattatcc ttgataccaa
    34741 ttggaatacc agcaaggacg ttgctttcat caatcccttt ttcatctaaa gcttttgcct
    34801 gttccaatgc tttttcttcg ttcaatgtaa tgaatgcctc gacctcaggc tctgtttctt
    34861 tgattcgttc aaatgttgtt tttgtcaaat cttgtacagt gatttctttc gatacaagca
    34921 tatgatgcag ttcttctaga gaacgattgt gtaattctgt cattatgcgc cagcctcccc
    34981 attatcgatg attgcgggta ctttgataaa gccgtcttct gtttccggca catttttcaa
    35041 taattcatcg cggttccatc cagcttgcgc ccgatcttgt cgcatcacgt ttactgtttc
    35101 aaccacattc gacgtaaatg gaacaccttc cgtatctact tcttccagca tttcaaccat
    35161 atcgatgatt ttacctaatt ggtcggtaaa acttgcaagt tcttcatctg cgaacgcaag
    35221 ttttgctaat ttggcaacgt gtttcacttg ttcttcacta atcgccatac taccccttct
    35281 ttctaaacga gttaaaaact ttgctctcta ttttaacaca agagtgccgt gtctgaaatt
    35341 gtttattgaa atttgtctga aaaaactcat ttttcattct tctactgagc gacttttagc
    35401 caatgcatct gccatttgat cttctgtcca aacttcgatt cctaattctt gtgctttggt
    35461 cagcttgctg ccagcatctt ctcctgctac cacgatatcc gtttttttcg atacgctacc
    35521 agtcactttc ccgccaagat tttcgatcgt ctcttttgct tcttcacgag taaatcgtgt
    35581 tagtttacca gtaaggacta ctgttttttc tttaaaagga gactcgactt cttgcaattg
    35641 ggcatttcga agccctttgt attcgaaatt gacgcctgct tgttttaatt cattcatcaa
    35701 ttcatggact tcttcgttac tgaaataagt gactacactg tcggcaatga tatcaccgat
    35761 cgtatcaagc gcaataatcg actcatagtc acttttactc aatgtttcta gatcaccgaa
    35821 atgttctgct aaaatttttg ctgctttcgc tccgacatgt cggattccta acccgaaaat
    35881 caaccgctcg accgaattat ctttgctgtt gtcgattgct gtcaaaatat tatttgctga
    35941 tttttctttt attttgtcca acgtcaataa ctcttcttct gtcaatttgt aaagatcagc
    36001 aacatctgct actagttttt tatcatacat ctgttctagt acgcgcggcc ccaacccatc
    36061 gatattcatc gcatttcttg aaacaaagtg gttcaaacct tctttcattt gagcagggca
    36121 tttaggattg atgcaccgta aggctacttc ttcatccaaa tggacaagtt cacttccaca
    36181 aactggacaa tgtgtaggca attggtattc ttcgctgtct ttaggtcgtt tatctaacac
    36241 gacttgcgaa acttctggga tgatgtctcc cgctttataa atcagaactg tatctttcaa
    36301 gcggatatcc ttcaacttaa tataatcacc gttatgcagg ctggcacggc ttactgtcgt
    36361 tcctgctact cggactggtg tcatgatagc tgttggcgtt actacccctg ttcgaccgac
    36421 tgtccattcg atgttttcga ttagcgtttc aacttcttct ggcggaaact tgtaagctgc
    36481 tgcccatctc ggtgctttga tagtaaaacc aagttgatct tgtagcgaga attcattcac
    36541 tttgatgacg attccatcga tttcataggg caaatccaca cgtttgtcat gatattcttc
    36601 aatgtatgac cacacttcat caatcgaatg acatagacgt ttttcccgat tcgtgtggaa
    36661 tccaattttt tctagctctt ctagcgcatc atactgggtt tttgcttgca taggaccgaa
    36721 atcagctact gtgtataaaa aagtgtccag atttcttttc gctgtgattt tagaatctaa
    36781 ctgacgcaaa gaaccagctg cggcgttccg aggattcgca aagatatctt ttccctctgc
    36841 ttctctgtct tggttcaatt ggacaaatga ccgttttggc atgaaacatt cgcctcttac
    36901 ttcgatgttc attggttctt ttaatttgat tgggaccgac cggacagttt ttaggttttc
    36961 agtgatgttt tctcctacag ttccatcccc gcgagtcgca cctctcacga aatttccatc
    37021 ttcataacgc aaagaaacgg aaagtccatc tatttttagt tcacagcaat agtcaactac
    37081 acgtcccact gctttttgta cacgttgatc aaaagctatc agctcttctt tgctgaaaac
    37141 gtcgttcaaa ctgtaaagcg gaatatcatg agtgactttt tcaaaacctt ctaatacttt
    37201 tccgcctact cgttgagtgg gagaatcaga cgtgatcaaa tcaggatatt gttcctcgat
    37261 tgctactaat tctgcgtatt ctttatcata aacatagtct tccactgttg gtttatcttc
    37321 tacgtagtat tcgcgagacc attggttcag tcttgtacga agttcttctg ctcgtttagc
    37381 agcttcgtca atcgtcattt catgttccat catttaccct ccactactaa gctttttcaa
    37441 ttggcgcaaa ggcagccaaa agacgtttta ctccttgact tgggaaagcc acatctagtt
    37501 ctagatcctt cgacgtcccg cttactcgga cgactgttcc tagaccccat tttttgtgtt
    37561 tgactttatc tccaggtttc cagctgttat tttcccctcc agtagcactt ttagagggaa
    37621 caaccgctgt ctctggctgt ttatagctat atgctgtttt tttagcgaat acaggcgttt
    37681 ttttaggcgt aggacgcatg ccttcgattt ccagtaactc ctgatcgatt tcttctacaa
    37741 aacgagaagg tcggttatat tgcgtccgtc catataatgt tctggagaaa gcatttgtta
    37801 aatacaacgc ttcttctgct cgcgtgatcc ccacatatgc gagacggcgt tcttcctcca
    37861 gttcgctttc ttccatcaat gctcgagata aaggaaaaac cccctcttct agaccaatca
    37921 ggaatacaac tggaaattct aatcctttgg ctgcatgcaa agtcatcaat gtcacttggg
    37981 aagcatcttc ttctaaattg tcaatgtcgg aaacaagtgc cagatcattt aagaagaccg
    38041 ttagtttttc ttctggtgca tcagcgtctt cttcattttg ctgttcgaat tgtttgtcga
    38101 attcttgtgt gaccgttaaa aattcctcca agttttcgat gcgtgcttgt gcttctaatg
    38161 tattttggat ttttaagtct tctagatatc cgctacgatc caaaacctct ttcgtcaatt
    38221 ctgtcactgt caagtatggg atcatctgag tgacttcttg gatcatctct ccaaatgcac
    38281 ctaattgctg tcctgctttt ccaccgatat ttgccagatc cacattttgc gctgcttcaa
    38341 gcagtggcca ttcatgaaga gaagcaaaac tgcgtagttt ttcgatagat cctggtccga
    38401 tgcctcgttt gggcgaatta acgatacgct caaaactgat tgaatcttga ggatttacca
    38461 gcacattcaa atacgctaaa atatctttga tttctttacg atcgtagaac ttgtgccctc
    38521 caaccatttt ataaggaata tttgctttca acagagtttc ttccattaca cgagattgtg
    38581 cgttcgtccg gtacaagatg gcaaagtcgc cgtaattccg atgattgctt cgaatctctt
    38641 cttgcatccg gtctacgata aaacgggttt cgtctcgttc attatctgca cggtagtaag
    38701 tgatcttgtt tccttctttg ttttctgtcc acaagttttt tggttttcga ttgctattgt
    38761 tttcaatgac ttggtttgct gcgctaagga tgtttttcgt ggagcgatag ttttgttcta
    38821 gcaaaattac tgcagcatct ggataatctt tttcaaaatc caagatgttc tgcatatctg
    38881 caccgcgcca gccgtaaata ctttgatctg catctcccac gacgcacagg ttcctaaatc
    38941 tacctgccaa taaattgact agcgtatact gtgcgtgatt tgtatcttgg tactcatcta
    39001 catgaatata atggaatttg ttttgataat acgtgagtga atcaggatgt tcttcaaata
    39061 atcgaatcgt attcatgatc aggtcgtcaa agtccataca ttgattatat tgaagctctt
    39121 tttggtaagc agcataacat tttgccgcaa tctcttcgta ataactgcct tgcatttttg
    39181 caaaatcctg aggggtctgc aggctgtttt ttgcttgaga gattgttcct aatatacttc
    39241 gaggatcata ttttttcgga tcaatgttca gttcttttag tatccgtttc atcaaggtca
    39301 gctgttcaga agaatcgatg attgtaaagt tccggttata cccgataaaa tcaacatctc
    39361 ttcttaaaat acgcacacac atcgagtgaa aagtcgatac ccagacatct tcgcctccag
    39421 atgcgagaat cgcattgaca cgttctttca tttcttttgc agccttattg gtaaaagtaa
    39481 tcgctaggat gttccaagga ttgacttctt tttcctcaat caaataggca atgcggtgtg
    39541 tcagcactct cgtctttcca cttcctgcac ctgccattag cagtaatggt ccgtcagtgt
    39601 gaagaacagc ttctttctga cgggggttca tcccgtttaa taattcagct ttggatgaca
    39661 ttaacaacat cctttctttt atcaatcttg ttaattatag cattttcttg gctttcatta
    39721 aactagctag ttcaaaatca tttgtttctt tttatggatt tttccttgtc aattcgttga
    39781 tttcgcgcta aattaaaaga aagcgaggcg tttgaatgga aaaaaaagga aaaatcaaga
    39841 aattagataa gactcagata tattttattc taaaaggaat atttgttgga ctgctgacag
    39901 gaatcgtcgt tagcctgttt cgattatcta tcgaaaagct aagcgattta gttcgaagta
    39961 tctatgaaat gagtcgggaa cagccgattt acttaattgg cctagctatt tgctgcatcc
    40021 ttgcagcatt tttcgtgggt tatctggtca aaaatgaacc ggatatcaag ggaagcggga
    40081 ttccacaagt agagggacag cttcgcgggg aattatcaat gaactggttc tctgttttat
    40141 ggaaaaagtt cattggcggt gtactttcag ttggtgcggg cctttttcta ggtagagaag
    40201 gaccttctat acagcttggc gcatccattg gacaaggagc tggacaattg tttcggagtc
    40261 catcttcaga agaaaagatt ttaatttcta gcggtgcaag tagcggactt gcagcagcat
    40321 tcaatgctcc tatcgcagga ttactctttg ttttagaaga aatccatcac tccttttctc
    40381 cacttgtttg gctgacatca tttgcttcag caatcactgc taacttcgtc tctctttact
    40441 ttttcggctt gagacctgtt ttatatctag gtgaccttcc ttctttgccg ttgaaatatt
    40501 atggttcctt agtgatctta ggagtacttc taggcatctt aggttttatt tatcagaatg
    40561 tcttgctggc tttgccaaaa ttttacaacc gattgccttt accagcttat tttcacgggt
    40621 tcgttccttt cttgctgatt ttgccaattg gttatttatg gcctagtact ctaggcggag
    40681 gcaatcaaat cgttttagct tttggacatg cttccttgcc aatcattatg attatcggtt
    40741 tgtttgttct tcgattcgtt ttttccatga tttcttacgg tgggggtctt ccagggggga
    40801 ttttccttcc gatattgaca ttgggggctt taatcggtag cttttatgga aacattatgg
    40861 ttgatttcgg gatggatcca atctacatca aggattttgt cgttattgcc atggctggtt
    40921 atttcacagc gattggaaaa gcaccgctga cagcaattat tttagtgaca gaaatggttg
    40981 gctctcttaa tcatctgatg cctttaggat tggctgctct tgttgcttat atcgtcaatg
    41041 accttctcgg cggtaatcca atttacgaat cattattgga acgtctactt ggaaatactc
    41101 agaccgcctc actaaaaggt gcaaggacaa cttttgattt tccagtaacg gcagagagtt
    41161 ctttagacgg attaatggta cgagacttca attggcctaa aaatatgctg ctaatctcta
    41221 tacgcagagg aaatcaggaa atcttgactc acggtgatac gatcatgaat gtaggagata
    41281 ttttggttat cttgactgat gaagcaaact tgccaagaat caagcaggaa atcgaggcta
    41341 aatcttctat cacaaatttg aagcaatcat tttaacgaac atggaaaaac tattttacaa
    41401 caactattca tctacactac ttgattctat atgattctat aaaaaaagaa gaggctggga
    41461 aaacccagcc tcttcttttt tatttctatg cttctgctac tgcaggagct tttctcaata
    41521 aaccataaat cacaccagat acgactgctc cgatagcaat aaacaccaaa tacatgaccg
    41581 ggtgactcag taagaataca acgaaaatcc cgccgtgagg agccagtaat ttgattccga
    41641 aagctccgac taatccgcca gttaaagcag aaccaataat aaaacttgga atggcacgaa
    41701 tcgggtcagc tgctgcaaat ggaatcgatc cttccgtgac aaacgatagt cccatcacga
    41761 tgttcgtcaa accagcatct tgatctgttt tgctgaattt attcttaaac aaacgtgtag
    41821 caacgaaaat tgccaatgga ggtaccattc ctccagccat aacagaagcc atcacgacac
    41881 ttccgccagt tgcaacagtc gtagctaaag ttgctgtacc aaacacataa gcggctttgt
    41941 tgattggacc gcctaaatct gccgccatca ttccagctaa taatgcacca agtaaagcag
    42001 cattcgaacc actcatactt gtcaagaaac cgttcaatgc atcattgatt gctttcatag
    42061 ggacattgac cagcaacatc aagaaaccag taatcagtaa gccaaacact ggataaaaga
    42121 ggatcgtttt aattccatcc aatgattttg gtaagttctt gaataattta cgtaaaaata
    42181 cgatgacata accagctaag aatccaccaa tcagtgcacc taagaatcct gcaccgcctg
    42241 tattagctaa tgcaccagca gcaaatccaa cgatcaaacc agggcggtca ccgatacttg
    42301 aagcaataaa tcctgctaat accggcagca tgaaaccaaa tgcagcttgt ccgatttggt
    42361 tgaaccagct agcagcttga ttgtaattcc ctagatttga taattgatca tgtggcacgc
    42421 caataaattg gtcgatcatg aaagaaagcg caatagcgat tcctccacca ataacgaaag
    42481 gtaacatgtg tgatacgccg ttcatcaaat ctttgtaaat tctttgtccg atagtaccat
    42541 ccgcatttcc ttcttctgaa ctgctttctt ttccatcacc atggaatact ggtgcagtac
    42601 cactaagggc tagattgatc aactcttctg gtttacggat accatcactg acaggacgat
    42661 tgatcagttc ttttccgtca aagcgattca tctctacttt tttgtctgct gcaacgatta
    42721 caccttctgc acgggcgatg tcttcagcag tcaatcggtt tttgattcct tctgaaccgt
    42781 tcgtttcgac tttgatctcc acacccattt ctttcgcttt tttcttcaaa gcatcttctg
    42841 ccatgtaagt gtgagcgatc cctgttggac aagctgtaac ggcaacaacg aattttcttt
    42901 ctggatgagc agaagcttct gcagcttgtg tttcttgttc tgctttttct tctgcttctt
    42961 tttgctcttc agctgtttgg aacaagtctt ggacttcttc tggtgtattt gcttctttta
    43021 attttcctac gaaatctgca tcaatcagca atcgagatag tgctgctaat gcttgcaaat
    43081 gtgtgtcgtt tgcgccttct ggagctgcga tcatgaaaaa cagataagtt ggctgtccgt
    43141 ctaaggcttc ataatctact cctttttggc tttttgcgaa taaaactgtt gcttctttaa
    43201 ctgcactgtt tttcgcgtga ggcatggcga ttccatcacc aagacctgta gaagtttgtg
    43261 cttcgcgggc tagaattcct tctttatatg tttcgatatc ggaaatccgg ccggcatcaa
    43321 acattttttg gaccatttca tcgattgcgc cttttttatc agtcgcttgc aaatccatga
    43381 tcatcgcttc tttgaccatt aaatccttga tattcatgat tatcctcctt gaaactaatc
    43441 aatttttgtg atttcaactt ctggcagcaa ctcatcaatc aattcgcgag ctgccaagtc
    43501 atcagaaaaa gctgttgcgc ttccgcaagc aactccccat ttaaaggctt cgattggatc
    43561 tttcgtacgg ctgtaagccc caataaagcc agcaatcatt gaatcccctg cacctactga
    43621 atttttcaat gggcgaacta atacattcgt tttataagtt ccttgttccg tgaagagcaa
    43681 agcaccgtct ccagccattg agatcagcac attctgtgcc ccttcagcta gtaatttttt
    43741 cccatacgga atgatttctt ctacagattg gaaggttgtg ttgtaaagct cagctaattc
    43801 gtgattgttt ggtttgatca acaacggttt cttaacaagt gcatttttta ggtcatcacc
    43861 tgttgtatca atcacgaaat ctgcaccttt ttctcgaatg atatcaatca gttcttgata
    43921 aaatcctttt cgcaggctag ctggcgcgct tcctgacaat acgacgatat ctccttgtcc
    43981 tactgttcct aatgcttgct tcaactcatc gatttcttca tctgaaatag atgggcctaa
    44041 gccattgatc tcagtttcag tgtctgattt tagtttgata ttgattcttg tatcttcttt
    44101 tacggttgtg aacaaagact ggatttgttc tttttcaagc cacttcgaaa taaattcgcc
    44161 agtgaaccct cctaaaaacc ctagtgctgt agattgagaa tcgatccgtt tcaaaatacg
    44221 ggaaacattg atcccttttc ctccaggtaa cttgaagtct tccttcattc ggttcaaatc
    44281 gcctagtttt aacccatcta cgcgtacgat ataatcaatt gatggattaa gtgttactgt
    44341 gtaaatcatt gtattgcctc cttaattgtt gtcttttcac ggaattcatt aacgaattct
    44401 aatggacaac gctcggttat gatcgtggct gcttccaaag gtgcaacaga ggtgaaggac
    44461 aactggttga atttggtatg atcaatcaat acaaatgtct cttcggaatt tttcatggct
    44521 aaacgtttaa cagctgcctc ttctggatcc ggagtcgtga atccttgttc aagatgggca
    44581 ccgttcattc ccataaatgc tttattaaaa tgaaaatttt ttagttgttc tatcgcatta
    44641 gatcctaaaa cagcattagt tgccagcttt atcgttccac ctaaaataat ggtagcaagc
    44701 tccaaatcaa ctaaatttgc cgcatgttga acggaatttg tcactactgt gatctgtttt
    44761 ccttgcagaa aaggaatcat ttctaacgta gtagagccag cgtctaaata aatgacatct
    44821 tcgtcattaa tggtttcagc tgccgatttg gcgatttgct gtttttcgtg aacgtttttg
    44881 agtgattttt cactcatatt ctgctcaaaa ttcaataatt gctctttctt agctccgcca
    44941 tgaactcttt ctagaaaccc actattctcc agctcttgta aatcacgtct tatcgttgat
    45001 tcagaagcat tcaaaaggtt tacaagatct tgtgatttca cgattttctg ctgttcaagc
    45061 aagtctagga ttttttgtct gcgttcctct gtaagcattt tcacgatcac ctcaatacaa
    45121 ataatagcat attttttttc tactagcaat ataaatcagt caaaaacgtt cataaaaatt
    45181 ataaatcagt caaaaaaaaa ataaaaaacc agtcagaaag actggttttt tatttttttg
    45241 tctatcatca agccgtttta tcgtttttta ttttcgcttc ttgttttttt catataaaca
    45301 gaagcaggtt ttctttcctt atgaagaaga taataataaa gtcccatccc gccaaacatg
    45361 accaaaaaac cgatgactga tcgaatagga aaattaggaa caaaaaagag acagacggat
    45421 aataatgatg ccggaaccat tattcgataa tagagttgtt tatccatttt cttcacctca
    45481 tattttttgt aaagtaatca attgttctaa ttctcggtaa tctaaaaatt gcacacgcag
    45541 atgctgctca cctttcttga catcgaattg gatacttcgc cgctttttct ttattcgttt
    45601 gaaggtaaca tcagagagtc tatgtttgat ttcctttaac ggtaattctg acagggattc
    45661 tgaaggaaca tagattctta aggataaaag atactcttga tatttttcat ctgttacctc
    45721 tacttctaga tattccctaa tataatagca ataagcttcg attccattat ccaaagcaac
    45781 agtcggatcg ataactttat agcacatcat gcgtgatcga ttctcgtcaa cccaaggaaa
    45841 atacgcctga gcatttagtg ttccgcgaac acgcctttct ttataatctc cttgttggat
    45901 atcataactt tttgttacaa aaaatgaagc attcttttta ggaacgggtt gcatttgtgc
    45961 attctgatac tggacagatt ggatcgtatg gtcagtgtaa atgtattcaa cgaatggcgg
    46021 tttcaataaa ccgacaccat tgtcccaagc aagatgatca aacccaagct ggtccctaaa
    46081 ataggattga aacatttgat tgtttggctt ttgccagtta aaggggtctc gataaagctg
    46141 gatacatatg acttcgtttt ctaattcctt cactttttct acttgttgga tgtcgagaaa
    46201 aatcggttta gggatgattt gatgataata gcgactataa tacatttccc aacacgaagt
    46261 gaaacataaa ccttggtaaa aaagatcgta tcctggaaat tggttgcaat cgactactac
    46321 ttccccgttt tgttgtttca ttttaggtaa atgttcgatt tcatcctttt tctcaaacaa
    46381 tagccgttga gagatctgtt ttgtattgtg atagataaac tcgctataag ctcgcatata
    46441 ggcgaacacc ccatgagcaa tcattttttt ctgaatgaag tctcgaatca aaaattgctt
    46501 ttctttgaaa acagagatag acaatacatt ttttatcaat aattgattat cttccaagcg
    46561 aaaactacat tcgttctcgt catttcctac aactaaccaa ctttcgtctc ttaccgatgc
    46621 tagcattatt ttttcttgaa gctttcgaag atcatacgga tccgcttgtt gtgtgaaaga
    46681 gcggataaat gttggctgga tcaacagacg tgtcaaaata gcccaatcgc ttatttcctg
    46741 ttttttttct tcttcattta agtctgtgag ctggatagtc atcaataatt tatcttcgcc
    46801 aatcattttc tcacctcttt caaatctttt tataatttaa ttataaccaa tgataaagaa
    46861 aaactaaaac tttatgtctt agaaaccaat ttggacgatt acatgataga ctaagtaaaa
    46921 agaaacaaag aggtgtttac aatggggttg aaatttgaac gtacagataa tttagataaa
    46981 cttttcgaat catttgctgt cgatccggaa aaaacagttg caccaaaaga agagatcgat
    47041 cgatttctaa aaccacagcc acaaaatgaa aagaaaaaag acaagaaaca aaagtaagtt
    47101 aagctcaaaa aa
//
LOCUS       DACEQH010000020        45850 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.020, whole genome shotgun sequence.
ACCESSION   DACEQH010000020 DACEQH010000000
VERSION     DACEQH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.020"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            591..1280
                     /locus_tag="IW833_001129"
     CDS             591..1280
                     /locus_tag="IW833_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1307260.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IW833_001130"
     CDS             1267..2475
                     /locus_tag="IW833_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1307261.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IW833_001131"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IW833_001131"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ1307262.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IW833_001132"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IW833_001132"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ1307263.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IW833_001133"
     CDS             complement(6578..7273)
                     /locus_tag="IW833_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ1307264.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLTPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IW833_001134"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IW833_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ1307265.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IW833_001135"
     CDS             complement(8472..8663)
                     /locus_tag="IW833_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ1307266.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IW833_001136"
     CDS             complement(8684..9574)
                     /locus_tag="IW833_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ1307267.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IW833_001137"
     CDS             complement(9561..10328)
                     /locus_tag="IW833_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1307268.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IW833_001138"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IW833_001138"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ1307269.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IW833_001139"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IW833_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ1307270.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEVQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IW833_001140"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IW833_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ1307271.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IW833_001141"
     CDS             14944..15282
                     /locus_tag="IW833_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307272.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IW833_001142"
     CDS             complement(15450..16208)
                     /locus_tag="IW833_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1307273.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IW833_001143"
     CDS             complement(16226..17044)
                     /locus_tag="IW833_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1307274.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IW833_001144"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IW833_001144"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ1307275.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IW833_001145"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IW833_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ1307276.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IW833_001146"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IW833_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ1307277.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IW833_001147"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IW833_001147"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ1307278.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IW833_001148"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IW833_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ1307279.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IW833_001149"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IW833_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ1307280.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IW833_001150"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IW833_001150"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ1307281.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IW833_001151"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IW833_001151"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ1307282.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDRAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="IW833_001152"
     CDS             27086..27385
                     /locus_tag="IW833_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ1307283.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="IW833_001153"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="IW833_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1307284.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="IW833_001154"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="IW833_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ1307285.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="IW833_001155"
     CDS             28352..30325
                     /locus_tag="IW833_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ1307286.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="IW833_001156"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="IW833_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ1307287.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="IW833_001157"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="IW833_001157"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ1307288.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNIIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="IW833_001158"
     CDS             33204..34496
                     /locus_tag="IW833_001158"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ1307289.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35283..36146
                     /locus_tag="IW833_001159"
     CDS             35283..36146
                     /locus_tag="IW833_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1307290.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36275..37897)
                     /locus_tag="IW833_001160"
     CDS             complement(36275..37897)
                     /locus_tag="IW833_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1307291.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38146..38667
                     /locus_tag="IW833_001161"
     CDS             38146..38667
                     /locus_tag="IW833_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307292.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38853..40583)
                     /gene="spxB"
                     /locus_tag="IW833_001162"
     CDS             complement(38853..40583)
                     /gene="spxB"
                     /locus_tag="IW833_001162"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ1307293.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40748..41566
                     /locus_tag="IW833_001163"
     CDS             40748..41566
                     /locus_tag="IW833_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ1307294.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41669..41866)
                     /locus_tag="IW833_001164"
     CDS             complement(41669..41866)
                     /locus_tag="IW833_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307295.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            42035..42613
                     /locus_tag="IW833_001165"
     CDS             42035..42613
                     /locus_tag="IW833_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1307296.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(43658..44161)
                     /locus_tag="IW833_001166"
     CDS             complement(43658..44161)
                     /locus_tag="IW833_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1307297.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44519..45544
                     /locus_tag="IW833_001167"
     CDS             44519..45544
                     /locus_tag="IW833_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1307298.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT        13990 a         8679 c         9019 g        14162 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggttgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtgtcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga cttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagaccgt gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    25441 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    25501 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    26881 aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac atcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgag tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
    34621 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca cagtcccttt
    34681 ttccgaacaa acggtgttca aaagcttgca tctacggcac taagcccaat cagatgaaaa
    34741 atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca gctttttcac
    34801 aacctctcca ctaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatattcaa
    34861 caaacatgag aagctgtttt ttgaaattgt tccttgtgac tcggagctaa acggcaggct
    34921 cacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg
    34981 caaaatatga ggaactattt tttccgtttt tttctctaat actcaggtca aaacgctgat
    35041 ttcacaacct ctgattcacg gtgttccaga agtagcttct acggaaataa gccgaaattt
    35101 cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt
    35161 ctgtcacaac ctcttatttt gattagaact tgcgaaaata aaccggttaa agtgtgtcta
    35221 gcgcaagaga tttgctataa tagacgataa gggtaaaatg taacgagaag gggttgaaaa
    35281 atgtgtatca aattgtaaca gattcttgtt gtgatctgcc ataccatgta ttagcaaaag
    35341 aaaaagttga ttttttatca atgaatctcc aattgaatga tcaagagtta gtagatgacc
    35401 tcggaaagac attcaattat gaccgctaca tgaatcagct gaaaaatggt gcgatgccta
    35461 cgacatcaca aatcaatgtt ggccgatatt tagaattttt ccgttcttat gcggaaaaac
    35521 agattcctgt tttatatgtg gctttttcca gtggattaag cggctcttac agtagtgcgc
    35581 ttcaagcggt tgaattgctg aaggaagaat atgaagaccc tgagatctat gtttttgata
    35641 caaaagctgc aagtattggg gaagggcttt tagtattgga agctgtaaga ttgaaaaaag
    35701 aaggtaaaag catcgaagaa accctagagt ggctggaaag taattatttg aaagtccatt
    35761 catgggtaac agtagacgat ctgaaatatc tagaacgagg cggtaggatt tccaaagcaa
    35821 gtgctactct tggcagtcta ttgagtgtaa agcctattat tacggtggat aaagaaggaa
    35881 ggctgacgaa tgttgacaaa gttcgaggaa gaaacaagtc gatccagaca atcgtcaatg
    35941 agacggtaaa aacaatcgtt gcaccacttg atcaaatcgt ttatgtggct tacgctgggg
    36001 atctagagag cgcggagaag gcaaaacggc ttttggaaga gcagataaag atgaaagatg
    36061 tgaagcttta tcccttagga ccaacgatcg ccagtcacac aggctatggc tgtatcgcga
    36121 ttttttcgat gggaatcagt cgataaaagc agtaactcat tctgtaaaaa agcccagaaa
    36181 ttgattgtaa caaaaacagc gactcttaaa agatagatga gcatcctatc tcttagaggt
    36241 cgctgttttt ttatttttta agctaaatag gccattatgc ccataactcg ttcaattgtt
    36301 tttgaatatc tgcattttcc aaaaattcat catatgtcgt atcagctcga tcgatcacac
    36361 ctttatccga aacggcaatg atgcggtttg ccaatgtttg gataaattga tggtcatgtg
    36421 acgcaaataa gattgagcca gtaaatgcca tcaagccgtc attcaatgct gtgattgatt
    36481 ctaagtctaa atggttcgtt ggatcatcta atacaagtac gttagctttc gaaagcatca
    36541 tttttgaaag catgcaacgg actttctctc caccagataa aacattcact ggtttcaata
    36601 cttcttcacc agaaaacaac attcttccta agaagcttct taagaaggtg ttatcatctt
    36661 cttctttact tgcatattga cgcaaccagt ctaaaatcgt tagatcagta tcgaattctt
    36721 tcgttgtatc ctttggtaaa taagaattac ttgttgtcac accccaacgt actgttccag
    36781 tatctggcgt aatttcaccc atgataactt tgaatagcgt tgttgttgtt atatcatttt
    36841 ctgcgacgaa agcgaccttg tcttctttgc tcaaattaaa agatatatca tctaaaattt
    36901 tctttccatc aatcgttaca cttacatttt ctacctgcaa taagtcgttt ccaatctcac
    36961 gttcaggttt gaatccaacg aatggataac gtctagaaga aggttggata tcgtctaaag
    37021 taattttttc aagcattttt ttacgtgaag ttgcttgttt tgattttgat gcattcgcac
    37081 taaagcgggc aataaattct tggagttctt tgatttgttc ttcttttttg gcatttgcgt
    37141 ttgcttgaag tttagccgct agttgactag attccaacca aaaatcataa tttcctacat
    37201 aaagtttgat tttaccaaaa tctagatccg ccatatgtgt acatactttg ttcaaaaagt
    37261 gacggtcatg ggatacaacg atcacagtat tttcaaaatt gatcaaaaat tcttccagcc
    37321 aattgatcga ttggatatcc agaccgtttg ttggttcgtc tagcaaaaga acatcaggtt
    37381 taccaaatag tgtttgtgct aataacactt ttaccttttg tccaccagtt aattcactca
    37441 tttgctgatg gtgcaattct tcagggatgt tcagaccttg cagtaaaaca gctgcttctg
    37501 gttcagcttc ccatccatta agctcagcaa attctccttc taattcagct gctcgaatac
    37561 catcttcgtc agtgaaatcc tctttcatgt aaatcgcatc tttttctttc agcacttcat
    37621 acagacgctt gtgtcccatg atgactgttt caagtacagt atagtcttca tagtcgtaat
    37681 ggttctgttt caatacggcc atccgttcat ctggtcccat agtaacggaa cctgtggaag
    37741 gctgcagatc ccctgataac actttaagga acgttgattt acctgcacca tttgcaccaa
    37801 tcaagccata gcagtttccg ggggtaaatt taatattcac atcatcaaat agtttgcggt
    37861 ctgaaaagtg caaactcaca tcatttacag taatcaattt ttttcctcac ttttatcatt
    37921 attagttttg acaagtctta tagaattata taggggaaag gtaaaagatt caaggcaatc
    37981 gaacattctt tgtgtttcac gtgaaacaga atgaaaaaaa taaaaataaa agcggagcga
    38041 aaatagctac tataggattt attagtttgc aacagcataa tttttgacgt tatttataag
    38101 agggagtact ataaaactgt aaaagaaatg aaagaaggat tgaaaatgga tgaaaaaaga
    38161 accatcgact ggggctcatt gattttggga attttatttg tacttgtctc attgttatca
    38221 tttcaagacc ctgtcggaaa tttagttgca attgtagtag tctttgcgat ttttgccttt
    38281 attaaaggga tatttgaact gtttgtacgg aacagattaa aagagctaac aggatataaa
    38341 ggaaagatgc ccttaataat cgggatcatc gatattctag ttggcgtctt tttcttattc
    38401 aatattggtg caggagtcgc agcattgcct tttgtttttg cagtatggtt tattgctgat
    38461 tcagttttag cgttatttac tgctgacttg gcaagaggag taagtgaagg atattattgg
    38521 tttacgatca tcgtcaatat tttaggaatt ttattaggaa ttttcttatt gtttaatcca
    38581 atttcttctg cgttgacatt aagcttttta gttggattct attttatgtt atttggtatc
    38641 acacatatcg tctatgcatt tagataagta aaatcaaaca aataaaaaag ccggcttccg
    38701 attacagttt tactgttgta attggaagcc ggctttttta tcatggacaa atatgcacga
    38761 ggtgattttt aggtgtcgag acaaatttat gcaaaaatgt gagaagctat atacacaaat
    38821 tttatcttaa tactcgaagc atagcgcagt tgtcacaacc tcttttcata taattcattc
    38881 aatactggca aatcatggac ttcatatttt tctttgaatg cttggatttc ttcttcagaa
    38941 tattttcttg gatctaaatg cagatcttct actggcaatg ggcgcttgtt attgatcttc
    39001 acatcgatta ctacaggacg atctgatttt gcagctgcat caaaggcaaa tcgaagttgc
    39061 gagtattctg tgactgtaaa gctatttgcc ccgagcgctt ctccaacttt accgaaatct
    39121 gcatcttcta aaaatacacc aaatttcttt tgttccgtat cttcttgttc tgcttcgatg
    39181 aaaccaaaag aatcattcga aaagacaaca ttaataattg gaagctgata tttgacttgt
    39241 gtaatgatat cttgcatgac catagcaaac ccgccatcac cacttaaggt aaatacttgt
    39301 ttttctgggt agcttagttg tgcggcaatt cctcctggca caccattacc catagttgcg
    39361 aaccatccag atgtcgtatg tttttgttta ccgttcatat tcaataaacg aatagaatgg
    39421 atagtcgtgt taccgacatc tgtgacgaaa atcgcatcgt cttcagcaat acgattgatt
    39481 tctttataga cagcctctgg acgaatcggt tcttcctctc gatcttcaaa agatttcaac
    39541 caatttaccc atttttcttt attttcttga tttgctaggt accagctatc agcagggcga
    39601 gcatccccta actcgacaag tctttgcaaa gctgtgtttg catcaccgag gatcgacaaa
    39661 gatgtttcat ggcgtcgtcc aaattttgac gcatcgatat cgatttggat aaattcagca
    39721 gttggattaa aaaatgcacg tccaaatggg aagtcgctac cgacaaataa gatcagatcg
    39781 gcttctgcca atgcttcatt tccgggttta gtagcgacac gtccagcaaa accaagaaag
    39841 ttttcataat catcaggaat aattcctttt gctagaacgg aagcgacaac aggaactgaa
    39901 aaatgttctg agaattcttt gatttgcgga aatccgtttc gcgttccttg accaacataa
    39961 agaactggtt tttttgcttt ttcaagataa ggcaaagcag ctagcaaatc tttttcttct
    40021 ggtaatatca cacttgtttt gtgagtgtga gcggtggaga aaggttgttc gtcaatttct
    40081 tcaaaaccaa gatcaacagg gatagtcacg atagccacgc ctttatgctc ataggcggct
    40141 ttaatggctt catctactac atgcggaaga ctttgtggtg tcatgactgt acgattgtat
    40201 acgctaacat ctgcgaaaat aggattttca tttagttcct ggaaagagtt atagttcatg
    40261 gatgtagaag caacttgtcc taagaacgct actagaggca catggtccat ctgtgcatca
    40321 tacaaaccat tgatcaaatg tgtcgcacca ggtcctgcag agccgaagac agcgccgact
    40381 ttccccgtta attttgcatc tgcagcggct gcgagtgcgc cgacttcttc atgacgaact
    40441 tgaatgtagt tgatttcggt tttttcttga tatagcgcat ccataattga attgaaagat
    40501 cctccgggaa tgccgtaaat atggtcgata ccccagcttt caagaacttt taccattgcg
    40561 attcctgcat tgatttttga catggtgttt cactccttta gcaatgatga tacataaatt
    40621 ttttgtatct agagtataga actttttatc gaaaatccca actaattcgt cttttcacaa
    40681 aaatgtagtc atgtttttgg gaatatgcta gagtagtaca tagatagatt caagagaggg
    40741 gaatggcatg gttgaatggc tattgagact tgaaccttgg cagcaagcac ttgctgggac
    40801 agcttttact tattttatga cagctcttgg tgccgggttg gtttttttct ttaaagaaat
    40861 caaaaaagaa gtgctaaact tgatgttagg atttgcttct ggtgtcatga ttgctgcaag
    40921 tttttggtct ttgttagatc cggctattac aaaagcagaa gaaaatggag atattgcttg
    40981 gctagttgta agtattgggt ttggtttagg cggtgtgttt ttatatatgg cagataaaac
    41041 actgccccat atgcattttg gaccacaaca tgaagcagaa ggattgccta cccatttaaa
    41101 acggacgatc ttgcttgttt tttctattac attgcataac attcctgaag gtttagccgt
    41161 gggggtagca ttcggtgcag cagcaactgc ggataatccg acagccgcta tattagccgc
    41221 tgtttcggtg gcgctaggta ttggtatcca aaacttccca gaaggagcag ctgtatcaat
    41281 cccgttgcga caagaaggtc tgagtcgtaa aaaggccttt gtttacggac aagcttctgg
    41341 aatcgttgaa ccaattgctg gagtgattgg agcgctacta gtaacgaaag tggagctgct
    41401 gcttccttat gcacttgctt ttgccgcagg agcaatgatt tatgtcgtgg tggaagaatt
    41461 gatccctgaa gcacagcaga cattgtccag caaacgtcat tttgcagttt ttggcgtcat
    41521 gagtggattt atcattatga tgattttgga tgtggcatta ggataaataa aaaaaatata
    41581 tatgatatat caaatagggc cgcgaactta tatcgcgacc ctattttttt agtaagttat
    41641 atcataatga agtgaatggt atcatacatt aataatagtt ttttctatgt gtatttcctt
    41701 tgatccagcg aacaagccag atgataagtg cgattgggat tagaactttg accgcaaacc
    41761 ataaaagccc gcttaggaca ctgacaaaaa gactgatgac gaagctagct gcaattagca
    41821 ctaaaagaac catgaagata tttaagaaca tatttttttg acgcatacaa aaccctcctc
    41881 ttgttcattc tttactatag aaaaagtata acagggattg tttaaaaaca cctccaactg
    41941 aagactgatt tttacttcat ctacttaaat gatcaaacaa ttgctttttc tctggtttat
    42001 catctaaaat agagcggaaa aggaggagaa tatcatgcct aaaaatcgtc ttgaagcatt
    42061 tacagatgct gtaatcgcga ttatcatgac tattttagtt ttagaacttc atgaacctaa
    42121 cggagacacg tgggaggcgc tcgctgcatt aggttaccga ttttttatct atattgtcag
    42181 ttttatcatt ttagcgattt attggaacaa tcatcatcat ttatttcaag ttgtacataa
    42241 gataaatgga cgggtcttat gggcgaacag tttctttgtt tttgcgcttt cgctttttcc
    42301 gtttgcaact tcatggatca gtgagtacat caatagcctg gctccagaaa tgacttatgg
    42361 ttttgttacc ttgaccgcta atattgcgta ttatatcttg gcaaaagaac ttgtcaaagc
    42421 aaataaaggc cacgagaaag cattgaaggc gatgttgaat aattacaaga aatcgtatct
    42481 atcgattggt ttgaatgtgt taggaattgt cttagggtat ttagtagcgc cagtagccgt
    42541 gctgatcgca aacatcgtga ttctagctgc ttggatcatc ccaagtcgta ccatcgaatc
    42601 acaatatcgg taaaatgatc cgtgtttaaa atttaagaga aaaatataga aaaaataaat
    42661 aaacgatcaa atatccgagt gatactagta ttcaaagagg gatgcagatg gatcaaaaaa
    42721 gaattcatgt aggactgatt actagttttg tcacgatgct tatttatttg gatcaaggtc
    42781 atttgacttg gacagtagtt ggtgtcgtag gatttttgac attattcaca gcaataaaaa
    42841 aataaacatc tttatgtaat ctgatgttct atctatttaa gaaaagcatg tttacagcag
    42901 tatgattctg ttgtaaacgt gtttttattt gtggaaaatt taatttttag aaaagtgtat
    42961 cgaaaaatag atagttagtt tttccacagt ggataacttc gtaagagata aaaataaaaa
    43021 attagtcaaa gaagggcgta tagatttctt ttgataggag atatacaaca gaaatgaata
    43081 aatattaacg tgttttcaaa cagtataaaa cggtgtggtt tttggaataa aaaaataaca
    43141 tatctcattt tacacttttg ttattttata cttaaaaaat aacgacacag aaaataccgt
    43201 gtctcagatt tctctactac ttctaaaaac tactgttttt ttcctttgaa atgccttcaa
    43261 aaagtaggat caggcagaat atactggcag aatcaaggag aagggagcct aaataaggag
    43321 aaaaaccata gaaaatataa agtaattact tataaaacaa gctttttata gaaaaataat
    43381 atacctgagt agggtataaa ttaagacgga attcctttct agattgagat attgactagg
    43441 aagaatgtta agattttaag cctttaagct aaaaaaaaac cctttttgtt aaataagggg
    43501 aatatagcct gtcagtttca aactgcaagt cgtcctgctg caataatata gtagattaga
    43561 gatgaaaata gattttttta aaccaatgac tgaataaaaa gaccgtcata gatacacatt
    43621 ggaatgtgtc tattacggtc tttttttacg aacgatttta ttgaagacgg acagttcggg
    43681 tcaaaaataa aaaagctacg atcagaaata agaataaaat gtatcccaaa ataatggcta
    43741 gacggtctaa cttatttgtc gatagatgtt tgtttagaag ttgataggcc cataaaccta
    43801 tcagtcctcc tgttgtatta gttatcaaat cagtgatgtc cgctacgcca ataccgaata
    43861 ggaattgaat caattctgcc aagaagctta caaggaaaat aatccctaat tttttagaaa
    43921 aagaaagtct cttgaaattg acattcaaaa gcaccccaaa aggaaaaaag acaatcaagt
    43981 tataagtggt ctcacgtaat accgtcgtat tttcaaaaga aaaagtagaa aaagggacga
    44041 gattgacatt gctgtaagaa taagtgagaa attgtggaag attaaatgat agcttgaata
    44101 aaatcaacca taatagaaag aataaataaa tccctaataa aaattttgat acataccgca
    44161 tagacatctc ctccatttaa aaagtatacc atcgaaaacc gctgaaaaat cataaaaaag
    44221 tatgaagttt aaggaccaac gtagttatcg tacgaaaatt agaagctttt tttgttggga
    44281 atccggtttc tttaacaaag ctaagatagc gagttgcttg gaaaagttgc tatctttttt
    44341 ctgtcaaaac gactcggaat gttcgttttt tgttctaatt tctccaagtt gtttactttt
    44401 tgatactaag tttatgaaaa atcaagaaat agttgttata ctaggtaatg ctgacaaacg
    44461 ggattggcat ttcatcttgg ttcagctttt tttccaatta ctaggaagga gggatagatt
    44521 gttcaagact tattttcgtt tcaataaaat acaagataat ccttttcgta tcatgaatct
    44581 gatgctactt atctttattg tattatggtt aggttacata aatcatgatt ttttgatttc
    44641 aagtttttca tcattaggga tctttacttt tttctattat caaaacatac cgatgaaata
    44701 tttgctgaaa cgtatgtttc tgatcggttg cggactgttg gttgctttcg ttttggggat
    44761 cttatcgact tatgttttat ggcttgaacc atttgccgta gctttggttg ctttttccag
    44821 cagattcgtg ttgcgattat tccatatttc taaaccaggt ggcttgtttt ttgcgatgct
    44881 ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa ttaccgatcg tctcacttta
    44941 tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg attacaaaat tactggattc
    45001 acggcctgag cagacaattg aaaaagcaac tttaaaagaa agatttcacg aagagccttt
    45061 agtgatcatt gattctgttt tctacagcgc tgccttattt ctttccgttt atgtcagtca
    45121 cggcttgaat ctgcataatc cttattggtt gaccttgtca tgtgcatcga ttttgctagc
    45181 agaaaacttg gatgcgatga agcatcgaca agtccaatat ctgattggat cgatgggtgg
    45241 gttatgtgtg tcggcattct tgtcatttgt gccgtttacg cagttgcaga ccatttttct
    45301 gatcactttt ctatatggaa tcgcacaatt tctagttgct agaaattatg cagttgctaa
    45361 tatttttctt aatccgatgg cgttgatgct atctacactt attcgtggag catacttgat
    45421 ttctttgatc gaatatcgtt ttttagggat cgtcatagga agtttcatcg gtttaggtgt
    45481 tgcgtgggta atgacggttg gtttgcaaca ttatttaacg gttgttcgtc aaaaattaga
    45541 atagaaggac aaatatatgt gccaaaagag gttatgacaa gatttttgtc acaacctcta
    45601 attcaaggtg ttcaaaagcc agattctcag cattaagtca aattcacaca aaaatgtgga
    45661 aaggaacttt caaaaaaatt ttatatttat tctatcaaaa aattttgttt atgatggtaa
    45721 ctgaataaac tttcctgaga tggtcaatgc tttccttgaa agtcttacag atattctttc
    45781 ttcatagaaa gtttaagttt tatagttagt gtacaataaa agtatgttta taccatatgg
    45841 tgtccggata
//
LOCUS       DACEQH010000021        41209 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.021, whole genome shotgun sequence.
ACCESSION   DACEQH010000021 DACEQH010000000
VERSION     DACEQH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.021"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(26..328)
                     /locus_tag="IW833_001168"
     CDS             complement(26..328)
                     /locus_tag="IW833_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1307299.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="IW833_001169"
     CDS             complement(353..2446)
                     /locus_tag="IW833_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1307300.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="IW833_001170"
     CDS             complement(2935..3699)
                     /locus_tag="IW833_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ1307301.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IW833_001171"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IW833_001171"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ1307302.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="IW833_001172"
     CDS             complement(4704..5780)
                     /locus_tag="IW833_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ1307303.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="IW833_001173"
     CDS             complement(5804..6397)
                     /locus_tag="IW833_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ1307304.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IW833_001174"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IW833_001174"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ1307305.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="IW833_001175"
     CDS             complement(8067..8366)
                     /locus_tag="IW833_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ1307306.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IW833_001176"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IW833_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ1307307.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="IW833_001177"
     CDS             complement(10121..11032)
                     /locus_tag="IW833_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1307308.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDRPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="IW833_001178"
     CDS             11152..12009
                     /locus_tag="IW833_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1307309.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="IW833_001179"
     CDS             complement(12196..12558)
                     /locus_tag="IW833_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307310.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="IW833_001180"
     CDS             12904..14343
                     /locus_tag="IW833_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1307311.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="IW833_001181"
     CDS             complement(14350..16902)
                     /locus_tag="IW833_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ1307312.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="IW833_001182"
     CDS             complement(17016..18347)
                     /locus_tag="IW833_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1307313.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="IW833_001183"
     CDS             complement(18313..19791)
                     /locus_tag="IW833_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1307314.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IW833_001184"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IW833_001184"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1307315.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="IW833_001185"
     CDS             22401..23828
                     /locus_tag="IW833_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1307316.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="IW833_001186"
     CDS             complement(23920..24285)
                     /locus_tag="IW833_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1307317.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="IW833_001187"
     CDS             complement(24282..24647)
                     /locus_tag="IW833_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1307318.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="IW833_001188"
     CDS             complement(24839..25087)
                     /locus_tag="IW833_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307319.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="IW833_001189"
     CDS             25086..25214
                     /locus_tag="IW833_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ1307320.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="IW833_001190"
     CDS             25271..26965
                     /locus_tag="IW833_001190"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ1307321.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            26969..27088
                     /locus_tag="IW833_001191"
                     /pseudo
     CDS             26969..27088
                     /locus_tag="IW833_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            27170..27361
                     /locus_tag="IW833_001192"
     CDS             27170..27361
                     /locus_tag="IW833_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307322.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            27739..28872
                     /locus_tag="IW833_001193"
     CDS             27739..28872
                     /locus_tag="IW833_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ1307323.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28903..29223
                     /locus_tag="IW833_001194"
     CDS             28903..29223
                     /locus_tag="IW833_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307324.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29252..29716)
                     /locus_tag="IW833_001195"
     CDS             complement(29252..29716)
                     /locus_tag="IW833_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1307325.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29847..31104)
                     /locus_tag="IW833_001196"
                     /pseudo
     CDS             complement(29847..31104)
                     /locus_tag="IW833_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(31133..31450)
                     /locus_tag="IW833_001197"
     CDS             complement(31133..31450)
                     /locus_tag="IW833_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1307326.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31476..31796)
                     /locus_tag="IW833_001198"
     CDS             complement(31476..31796)
                     /locus_tag="IW833_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1307327.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(32260..33675)
                     /locus_tag="IW833_001199"
     CDS             complement(32260..33675)
                     /locus_tag="IW833_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1307328.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(33692..35008)
                     /locus_tag="IW833_001200"
     CDS             complement(33692..35008)
                     /locus_tag="IW833_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1307329.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35158..35856)
                     /locus_tag="IW833_001201"
     CDS             complement(35158..35856)
                     /locus_tag="IW833_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ1307330.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(36145..37134)
                     /locus_tag="IW833_001202"
     CDS             complement(36145..37134)
                     /locus_tag="IW833_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ1307331.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(37214..38047)
                     /gene="msrA"
                     /locus_tag="IW833_001203"
     CDS             complement(37214..38047)
                     /gene="msrA"
                     /locus_tag="IW833_001203"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1307332.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(38222..39856)
                     /locus_tag="IW833_001204"
     CDS             complement(38222..39856)
                     /locus_tag="IW833_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307333.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(39843..41177)
                     /locus_tag="IW833_001205"
     CDS             complement(39843..41177)
                     /locus_tag="IW833_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307334.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        12504 a         8476 c         6959 g        13270 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga cggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctatcgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcagatgcaa gcttttgaac accgtttacc aagaggttgt gaaaaagctg ccctgcatca
    27121 agtaataaga acacccaagc taaaactgct tcttatgttt ttgacttggt tggacttatt
    27181 accgcagatg caagcttttg aacaccattt accaagaggt tatgaaaaag ctgccctgca
    27241 tcaagtaata agagcagcca agctaaaaca gcttcttatg tttttgactt ggttagactt
    27301 attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aaggcgttta
    27361 gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc ataaatttga
    27421 cttaataccg agaaactgat cccttctgtc ataacctctt tttatttatg tctgtttctt
    27481 tttgaaaaca actatttttt caacgtttac tctcttgcca atccaacatc atctaacgga
    27541 tacaactatt tcaatgatta ccattacact aaaaaatcgg aaacacacct ctccgaacac
    27601 aaatacataa gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg
    27661 taagatctcc tttaaaatga aattatctgt agtactaata atgcagacaa ttttatgaat
    27721 ctgaaaggag catctccaat gaaagcagtc acttggcaag gaaaacagaa aatggaaatc
    27781 agaaacgtag atgatccaaa aatattagaa ccaacagatg caatcatccg tatcacagca
    27841 acagccattt gcggctccga tctgcatctt taccatcatg gtgaagcagt tttggaaaaa
    27901 gggtatgttg taggacacga accaatggga atcgttgaag aagtgggatc tcaagttcaa
    27961 acattgaaaa aagggcaacg tgtggttatc ccttttaata ttagttgtgg tcattgtcat
    28021 ttctgcctga atcacatgga aagccaatgt gatgaatcaa atccagatca gttatatggc
    28081 gggcttttcg gatttggcaa gttgaacggt aatcattggg gtggtcaagc ggaatatcta
    28141 agagttcctt atgcagactc tacttcattt gttgtaccag ataatgatct tcctgatgaa
    28201 aaagttttat ttctatcaga tattttgccc acagcttatt ggagcgttga aaacgcagga
    28261 gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc cagtcggtct attcgctcaa
    28321 aaattcgctg ttatggctgg agcagagcgc gtaatcgcag tagatccagt acagcaccgt
    28381 ctagataaag cggctaatta caatggggtt gaaacttatt tcttagaaga cacggcacaa
    28441 gctggaacag aattgtatga actggctaaa ggtggcgcag atgtcattat cgattgtgta
    28501 ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag cttacaaagc
    28561 ggcacaatct ctccattaca gatggcatcg caagctgtca aaaaatttgg aacagtccaa
    28621 gtaactggtg tttatatgac tccagcttct tcttacccgc tgcaagaatt ttttatgcgg
    28681 gatatcgctg tcaagcatgg acaggcacca attatccatc taatgccaaa aatttacgca
    28741 atgattgcag aaggactatt cgatcctaca caaatcatta ctcattcgct gccactatca
    28801 gaagcagcac aagcatatga gatctttgac aaaaaagaag ataaaaatat aaaagttgtt
    28861 ttgaaacctt gaattcagta aaaaatacag gtgattttct ttatgcccaa aatcaaacga
    28921 aatacccatg agatcgaaat aggaaaagaa gaagatatcg aaatacgagg acgtcgcttc
    28981 cgcttctatt tccaaaatcg ttacacattg atctcactgg ctgtcgattt attaaccggg
    29041 attttctata tttttggaag ccttgcttcc ttaactaaaa tccccgatcg atatggcacc
    29101 tatttttatt tggctggtgc tattttcttg acgattcgtc ctattctcag aatcgtaagg
    29161 aacattttta tttacgacga aaaaaaagca atgaagagaa caagcaaaaa aaaagaggca
    29221 tgagaatttt tcaaatctca tgcctctttt ttcaccctat cgtatcactt tgatacagac
    29281 gagcggtcgt cccactttct tccttgacca ttgtcaaaaa tgtccactcg tatcgttcat
    29341 aaaacgtggt atgatctgta attaagtaaa gtttttcgta cccaagttga tgcatttctt
    29401 ttctgatttc cgctaatatc cgcttggcta tcccctgctt acgcttttcc ggttcaacaa
    29461 acagggcaca aagatttggt gtcagatcag ggcggttatg gaagtcgttt tcaatgacgc
    29521 ccgctcctcc gacgatggtt tctttttcat ctaagaccac ataccattga ggaatacttt
    29581 ccttattagc gattccagta cgaatacttt cctcatacac aacagcagga actccccatt
    29641 ttgatgaaaa ccaattcgcg ctttgtttta ctaattctgg gtgttctctt aatttgatcc
    29701 agttatatgt aaccaatgtt taaacctccg actaaaataa aacgtttaca aaaattatac
    29761 aagtctttat gaaaattagc aatgacaaca atagtgatgt aaaaaaaaaa cggaaaacaa
    29821 tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg
    29881 atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg
    29941 gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt
    30001 acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca
    30061 acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg
    30121 ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag
    30181 tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa
    30241 cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg
    30301 ataaacggtg cgctatgccc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca
    30361 ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag
    30421 aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat
    30481 ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa
    30541 attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat
    30601 gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg
    30661 cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg
    30721 tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct
    30781 gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gcaagaaagc
    30841 aatcaagaca gaaagaatgg caaatgtacc gttccaagca gcagccccga atcctttcca
    30901 tgattcacca ccaaaaattg agttcatcaa ttcttggaac ccgggaatcg gtaagttatt
    30961 gatcattact gctaatgctc ctaagatcat taacggcata ctcaccataa atgagtcccg
    31021 aatcgcaact aaatgccgtt gtgcaccaat ttttgatgct acaggtataa aatgtttctc
    31081 cataaatgca acaaatttat ccataaatct tccctctttt ctttactgta gattacgcgt
    31141 tcaatcgacg atacagatca atgatctctt ttgctaaatc tgtaaaagct atagatgtca
    31201 tcagatgatc ttgactgtgg accgtcaaaa gcgtgacttc aacgtgattt ccttgtgctt
    31261 cttgtgtcag catctctgtt tgagagtgat gtgcttggac taaagattcc tctgcatctt
    31321 tgattttttc atctgctaaa gtgaaatcac cttttttcgc agcagcaatc gcttccattg
    31381 catcgctttt tgcatttccg ccaaacatga tcagacccat gatggcttct agattttttt
    31441 gatcttccat ttattgtatc ctccgataat ttttattatt tgtttattag tttttctgct
    31501 tggtcgagta ctttttcacc attcatcatg ccatagtcag ccatattgat tacatctaaa
    31561 ggaatgcctt taggtgctag cttttcagca aattgatttt gcataaagcg tacttgcggt
    31621 cctaaaagta aaacgtctac atttttagat tctagttgat tatccgcatc tgaagcagat
    31681 acagcaaaga tgtctgcttc cattcctttt gcttctgctg ccttttgcat ttttgtgact
    31741 aggagacttg tactcattcc agctgaacat acaagcatga ttgttttctt ttccatttta
    31801 aacacgttcc tttctatact tacacttgta tataagcaag tagcgtgcca actttaaaag
    31861 cgctttaaaa agttgatatc atcacattta aggtctatac caagttgcat acacactaaa
    31921 aaagaaagcg cacactaaac acacaaatta atttttatta ccttttttct tttaacgtgc
    31981 acaaaaaaag ttgtgacaca aaacttgtca caactttttt caaataaacg gtattcaaaa
    32041 gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca taagatgttg tttttacttt
    32101 gctattctta ttacttgatg caggacagct ttttcacaac ctctcaataa acggtgttca
    32161 atcagctgac cctgggtatt agctcaaaac gctggattca caacctcttt tatttcaaat
    32221 aaccagtaaa tacaatgaac atctacaaaa aacttctatt tatttataga tttcgtcagg
    32281 caaatccaat tctctcttat caatcaaagt ctgataccac gaagcagatt ttttcaatgt
    32341 tctgcttcgt tcatcgttca attcgattcg aactaatcca tatcgattct taaatgcatt
    32401 cattggtgac acacagtcag tgaatgccca taacatatag ccttcgcaat ttgcgccttc
    32461 ttctgttgct ttgatcaagt aatgcaaatg ctgtccgata aattcgatac ggtaatcatc
    32521 ttgcacgatt cctttctcat ccatatactg ttcttcatat tctcttccca taccgttttc
    32581 tgttatcagc caagggatat tatgataatt ttcttttaga tacatggcta tatcatacat
    32641 gattttcgga tagatttccc atcccctgga attattcatt ttcttcccag gtaaatcaaa
    32701 gacttcataa tatcgttcag gatggaaagg tgtctcttca ttccattgat aacgaggggc
    32761 gcgaacgcgt ttaggataat attgattgat tccaaggaaa tctactgtat gatgctgaat
    32821 cgtttctaaa tcctctgttg caggatcgaa ataaatcgat tgtttttgac acaactccag
    32881 gaactcttct gtgtagtatc cttttaccat cggatcaaaa aatactcgat tataaaacaa
    32941 atcatacata taagcagctt tttggtcttc tggagaattg gatcgagcat agaccatttc
    33001 aggatttaaa atacagccaa tccttccacc ataatttccg ctgcggtaaa tctctactgc
    33061 tttagccgtt gctaaaactt tatgataatt ccacatcatc cacttttttg tgttttgttc
    33121 atgcggccag cgaacagcat ccaaatagca acgcgtttgg ggaacgattg gttcgttgaa
    33181 tgtaaaccag ttttttaccc tatcaccgta acgatcaaag gcaatttgag catacttagc
    33241 ataaaaatca actacttttc ttgaagacca gccatcatat ttttccaaaa gaaaagctgg
    33301 gacttcataa tgttccaaac aaatcatcgg ttctacgtct gcttcgatca aagagtcgat
    33361 catttcactt atatgctgag catattcttc atctactata cagttctcat aatcagtaaa
    33421 aaaacgagcc caattgatag aagtccgata atgtgtcaga tggatggatt tcatcagatc
    33481 cacatcctgc tggtattgtt ccataaaatt agttgctacg gctgggccat acccattatg
    33541 ccaaacaaaa tgttcatttt tataccagct atctataaac gaatcctgtc cttctttttt
    33601 tcctttccaa ccttccgttt gccaagcaga agatgcagca ccaagaataa aattttttgg
    33661 aatagtaatt ttcatgattt cctcctgttt tttagatccc atcattttcc acacgatttg
    33721 ctacttttac aaatggcaga tagatcaaaa tagaaacaat aatacaaaca agttgcgtaa
    33781 taactgcacc gatatttcct gctgttgcca gataagcatt gacgatcggc ggcattggcc
    33841 aagggaccat tacaactgct ttcgccgcaa atcctaaagc ggttgcaaaa taaccaatcg
    33901 ttccagtgat aagaggcgtt aagataaacg gaatagctaa aattggattc agcatgatcg
    33961 gcataccaaa aataactggt tcattgatgt tgaagatccc aggagcaagg gataacttgg
    34021 tgatttgttt catatcttcc cgttttccaa ctaacaatac tgcaatcagt aaaccgatcg
    34081 tgactccaga cccgcccata ctcatataca tatcccaaaa tggcatagta ataatgttcg
    34141 gtacttcttt tccagcttca aaagcttccg tatttacagc aattgccgct aaaagcaacg
    34201 gttcccgtat cggcttcacc atttgatttc catggatacc aatcacccaa aagacttgcg
    34261 caataaacat caatagcaaa atacccggta acccttgtac gacaccttct aatgggcgtt
    34321 gtacgacatt gtaaatgata tcataaaggt acattcctgt aagttctttg atgataaatc
    34381 ccgcagccgc gatacccgat acagtcagaa tcgtcgggaa taaagcagaa aaactagcag
    34441 aaacattcgt tggcacggta tcaggcattc tcagctgtag tttcttctgt ttcctcaacc
    34501 agcaataaag ttcaatagaa aggatggaga ccaacattcc caaaaataac cctttcgtat
    34561 ctgtatattg tttagccaat acgttttcta ctataacagt ctgattttca ttcactaatt
    34621 cataaacagt tggattaaca gataaataag acatcactgc gatgatacca gggaatacac
    34681 catccacttt attcagcttg gctaattcga tccctatcag aaaaacagca tagatggtca
    34741 agaaacttag tgtgacatag ctgatggcag atgcgatagg ctttaattct tctaaaaaac
    34801 gcaagaaact tatctgtgct aagccatttt ccgagtcaaa gaccattgct gaaaacaatg
    34861 tgccaaaagc acccgtaata attaccggaa tcaatgcagc aaatgcgttt tttatcacca
    34921 tgatatacct taaattattg attctcgttg ccacgttccc taaaacattg gctaatttat
    34981 ccataaaact cattccagat cccctcattc ctgcttcttc tccttgacgt catctctttt
    35041 tgtctattag tcagcgctta catttcgatg aacagaaact atttactcct cttttttacc
    35101 aaatataaaa caaaaattca tctgatgtat ttatcataaa atacatcaga tgaactgtca
    35161 attagtttct ttttctccta ttattttctg taattctctc cgatttattg ccatatggac
    35221 agtcattgca tcaaacgcag caagtgcatt ccgcctttcg atggcctgaa cgatctctgt
    35281 atgaatatcg atcgtctcga ttttcagatg ctggatatca taatttttat taaacaaagc
    35341 aattgattca ttaatgattg ggataatatg cagcaaggct acgtttccgc tagcttctgc
    35401 aatcatcgta tgaaaatcga tgtctaattg aacatgttct tgtgtgcctt ctcgaaaaga
    35461 agtttcaatc ttttgcgtta attttttcat ttggacgatt tgattcttcg ttgcatattc
    35521 cgctgccaaa cctgccatct taggctccaa taaatatctc agttcaaata agtctcgtat
    35581 catccgatcc tgatcttcaa tcaaagaaaa accgagtggg tcattggcaa tccctcgctt
    35641 atcactgaca aatgtccccg ctccttgccg aacttcaagg atattcctgg aagctagagc
    35701 ccgcacagct tctcgcaccg tgcttcgccc cacctctaaa taatcggaca attcatattc
    35761 attcggcagt ttatctccta cttgcaactg catttccttt aaatatctaa ggatcttgtc
    35821 agtagttact tctactaggg ttttattttc ttccaaacca catacctcct tttcatctga
    35881 tgtatttttt cgtaattatt ataacacgtt tgaatcatct gatgattttt tgtatacaaa
    35941 attgattaat ccgttcatag acatacaaac gaagaagata tttaccataa aaaagactag
    36001 gataaaattc actcctagcc ttcgatcatt tttattttga acgacaaaaa atatgatgtt
    36061 caataacaga cgatcttcat tcgattcttg atgtcatctt ttgttttttt taatatatgg
    36121 ataaaaagtt tgattacatc gtttctattc tagaatttgg tcgattctca attcttcttc
    36181 tggtgagaaa tctaaattat ccatcattcg aacactttct tgtaactgac ttaaccgact
    36241 tgctccgacc agtacacttg taacagattt ttgtcgcaaa ttccatgcaa gggccatttg
    36301 cgctaatgat tgtcctcgtg atacggcgat tgtttgcagt gctttgattt tttccagagt
    36361 tgatccaact tgttcttctg aaaggaatgg tatttcctta cgatgtgctc tagaatcttc
    36421 aggaatacca tgtagataac gatttgtcag caacccttga gcaagcggac tgaacgtgat
    36481 tgcccctagc ccttcttctt ctaatactgg agatagatcg tcttctagta gtgcacgaga
    36541 aaacatattg tagcgcatct gatgaataat gaacggtgct tcctttcttt ttaaaatttc
    36601 agtcattttt tttgtatctt ctccattata gttggatata ccaatatata atgcttttcc
    36661 ttgtcttaca agttgcatca aagcttctgc tgtttcttcc aaaggtgtat cagggtctgg
    36721 gcgatgatga tagaagatgt caacataatc tagccccatt ctttggagac tttggtcaca
    36781 acttgaaatg atggatttac gagagcccca ttccccatat ggcccttccc acatatgata
    36841 acctgctttg ctagaaataa tcagttcatc acggtaggct tgaaaatctt ctctaaaaat
    36901 acgcccaaag tttttctctg cagctccagc agggcctcca taattattcg caaggtcgaa
    36961 atgggtaatt cccatgtcga atgcgcctct taaaatctct ctttgattat ggataggatc
    37021 ataatcacca aaattttgcc ataatccgag agataaaatc ggtaatttca aacctgaatg
    37081 accggattta cgatattcca ttttttcata acgtgcacta tctgcaatat acaatttttc
    37141 tcctcctcat caaatctttt taaaacgctt tcttttttct atttttagtt tactacattt
    37201 taaagaaaag gagttaatca aaatgatttt ttcgtatatt tttttgccat ttgtttttta
    37261 ctcgttgata gatcagaaat tgattacgtg cttgctggat ttttttataa cgtttcggtt
    37321 gttttttgta aaattgctga tgatagtttt ctgctggcca aaacgtaccg gctggttcga
    37381 tttttgtgac gattggttgg tggaaagttc ctgaactgtc tagtttctgt tttgattgtt
    37441 ccgctaattc tttttgtcgt tcgccttgat aaaaaatcac tggacgatat tgcttccctc
    37501 tatcttgaaa ttgtccaaat gtatctgtgg gatcagtaat ttgccaatag attgtcagca
    37561 actcgctata acttatttct cttgtgtcat aaatgatctc cacagcttcc acatgacctg
    37621 tgtaaccacc acttacttga tcataatttg gtttttcaac atgtccgccc gtgtatcctg
    37681 acaaaacaga taggatccct tttttcgatt caaacggctc gaccatacac cagaaacatc
    37741 cccctgcaaa tactgcacgt tcttgatagt cagcatcttg catggcatgc ttggaaaagt
    37801 catatttttg ctccttatca aatccttccg taatcaataa ataaaagtcc attacatcag
    37861 gcgtcagatt cattcggata gctaatggac gtagtgttgc ttctagtttt gataactgtt
    37921 ctttaacgga tatattttct cttatgtcat tctttgtctg aatcaatact tttcgttccc
    37981 aatctcgtgt attcggattc aaaatcagat tataaagtgt ttgaatgaat atttgttcct
    38041 catccataga ttctcctcct ttccgaagaa gacaagctag tgactttagc tgtaatttat
    38101 cataaagtga ggaaataagc gattacttca tccccctaca gatttttcgt ataaaaaaga
    38161 gagaatcctt atcgacagat tctccttttt aatcaattta ttttataaat gaaaagttaa
    38221 tttacctttt atggaaaact attttaaaaa ataaacataa atatgcagtt aatgtaacaa
    38281 gataagaaat aatagtcgtt actgtataac ttgtgcttac tttaatatca gcaactcctt
    38341 tgactccgct tatttgtacc aatccattag cattcacctg ctcctttaca ggttgatgat
    38401 ttatttctac attcgttcct aaataagcat atacaggaag tgtgattact tggctcttct
    38461 cattagttac tctatatatg atagaactac cagttgtttt ataaggaagg tttacccatt
    38521 gactgccaat ctttgcttcg tgttttatta tatgctcctt attctccaat gaagcgatag
    38581 gagaataatc atataaaaaa taatccattc ctttagtggt atacgcctct actgtttctt
    38641 tgtttacaac aatatgtccc ggctgagaaa ttattttttg attgaaattt tttttactag
    38701 aaacagttaa agtgattaat aacaaaacag tcataaataa ataaaatagt tctcttgtct
    38761 tattgtatct tctatttttg aaaaatatca tcgttaatat cagagaagag aatattattt
    38821 gcaagcttaa gatccgccaa ggaaattgta acaatttggc tggcgtattt tgcagaagat
    38881 accatggcac caaactagtt gtagctagcc aagtcaaaca cgtaccataa taaatctttt
    38941 tttctatgtt tgttaatttt ttaaataaaa tcaggggaat acataaagat aacagaccaa
    39001 ctagtcccat tgagtaagta gttaagtcgt tgttcatagc ttcagtcaga ctatcgaaca
    39061 cattagatgc atgagcatat aaatttgtca cgctaggttt gttgattttt tggtagagca
    39121 tttgttcaaa catcggatac caaaaagcac tcgtcagaac caatgtcatt cctgccgcac
    39181 taaataattt tttaaatata gcaataaaat ctgcattctt gaaaccttta gaaaccaaac
    39241 gaataaaaat aaaaatgata attaatgctg tactcattaa tgttgatagt acatggctgt
    39301 acactagcaa actcatactc aatgctagct ttacagacgg atacttttct tcttttccag
    39361 agatgatttg ataagcataa aataatacag ggataacaag tgttattgcg atagcttcgg
    39421 caattgcgga acgatagtag atgtctactg tccgataagc agagaaaata tagagacaac
    39481 taaataaaaa tgccgctttt ttttgtttta aaaaccgctc gccataatga taagctacga
    39541 gtgttgtaca caagttcaat aaccaaacgt aaataatata gcttacaatc aaattttttg
    39601 tcatccaata taacattaca gctggaaaaa aagtcagaaa gggataaaaa taatttacac
    39661 catatccagt ataattgaat gtctcataat taatcggact ggtaaaaata gtcgataacc
    39721 ccttgattcg agataagtga aaagaaagat ctccagtggt ataaatactc tgtgtatttt
    39781 tgaaaaagat gaaagaatat atactactaa ctataattaa tccactcaat attactactt
    39841 ttttatttat tttcattctt cttgtttatc tcctttttag tcacatatcg agaatctttc
    39901 aacttattag gtgcaaatcg tttaaaactt gaatacacca ctaaacaata agtcagaata
    39961 ctgatccata aagccatgag agcagttgga gtatattttg ttgtaatttc tatcttattt
    40021 tctccagcat ttacatccac attcactaag ccatgttttt tataagagtc atttacgact
    40081 ttaccgttta cttttacctg tgtacctaaa taagcatata ctggtactgt cactttttgc
    40141 tttttgttgc ttgccactgt gtaagtaatg ttattatctg cagctttata cttccctttt
    40201 tcccacgatt ctcctacttt tacttcatgt ttttctaagt gaggcctttc ttttatcgct
    40261 tctgttgggg cgtaatcgta taatcctccc atatctgatg ttgtataacg tgggagtgtt
    40321 tcttcattga taacaatatg accgtgtttt gattggataa cttttgcgta attttctttt
    40381 gctgctatag acgtgacgag tagaaacacc acagtagcgc caatgattaa ccatgaatat
    40441 tttttattca tagtcttttt cataaaaaca agcgttatga tcattgaaga aaaaaagatt
    40501 tggataccta agatacgcca aggaaactgg agcaagctta tcggtgtttt ttgaaacaaa
    40561 aaccatggaa ataaattagt tacgcatacc catgtagaaa ctacgccaac atagatgatt
    40621 ttttcttttt tctccatttc tttcagtatg aataaaggaa aaatcaacgc aaccaatcct
    40681 aataacccca tagtgtatgt tgtaatatcg ttatttaacg cttgaatgat actatctgaa
    40741 atagaaagtg cttctagctg caaatctgtt ttgaagggac tgtgtatttc ttgataattc
    40801 atctgctgca acataggata ccaaaaatag ctagttaatg cacaagtcaa aaaaacagcc
    40861 tttactaaat tcacgaccac aaattttata tcgtttttta tttgcttgct taaaaacaaa
    40921 cgaactataa tcatacaagc aatcaatgca ctacacaata gtgtagttaa gacatgagta
    40981 tacacaagta gacttatact gacagataaa gcgatgactt ttttttctct attgaatatc
    41041 aatccataag cataatataa aacagctggg ataatcgtaa gagcgattgc ttctgcaatt
    41101 gcggatcgat aatagatatc aactgttcga taagttgcaa aagtatataa acaactaaat
    41161 aaaaaagcag catctatctt tttaaaaaac ttttctccat aataaaaac
//
LOCUS       DACEQH010000022        39550 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.022, whole genome shotgun sequence.
ACCESSION   DACEQH010000022 DACEQH010000000
VERSION     DACEQH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.022"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..132)
                     /locus_tag="IW833_001206"
     CDS             complement(<1..132)
                     /locus_tag="IW833_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1307335.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..841
                     /locus_tag="IW833_001207"
     CDS             542..841
                     /locus_tag="IW833_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1307336.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            914..2275
                     /locus_tag="IW833_001208"
     CDS             914..2275
                     /locus_tag="IW833_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1307337.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            2297..2557
                     /locus_tag="IW833_001209"
     CDS             2297..2557
                     /locus_tag="IW833_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307338.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2571..3323
                     /locus_tag="IW833_001210"
     CDS             2571..3323
                     /locus_tag="IW833_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ1307339.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(3417..4022)
                     /locus_tag="IW833_001211"
     CDS             complement(3417..4022)
                     /locus_tag="IW833_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1307340.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(4075..5049)
                     /locus_tag="IW833_001212"
     CDS             complement(4075..5049)
                     /locus_tag="IW833_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ1307341.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(5427..6206)
                     /locus_tag="IW833_001213"
     CDS             complement(5427..6206)
                     /locus_tag="IW833_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1307342.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(6273..7544)
                     /gene="celB"
                     /locus_tag="IW833_001214"
     CDS             complement(6273..7544)
                     /gene="celB"
                     /locus_tag="IW833_001214"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1307343.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7697..9094
                     /locus_tag="IW833_001215"
     CDS             7697..9094
                     /locus_tag="IW833_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1307344.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            9175..9615
                     /locus_tag="IW833_001216"
     CDS             9175..9615
                     /locus_tag="IW833_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1307345.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9763..10089
                     /locus_tag="IW833_001217"
     CDS             9763..10089
                     /locus_tag="IW833_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1307346.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            10090..10839
                     /locus_tag="IW833_001218"
     CDS             10090..10839
                     /locus_tag="IW833_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307347.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10919..11374)
                     /locus_tag="IW833_001219"
     CDS             complement(10919..11374)
                     /locus_tag="IW833_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1307348.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11801..12076)
                     /locus_tag="IW833_001220"
     CDS             complement(11801..12076)
                     /locus_tag="IW833_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307349.1"
                     /translation="MKLEDKKVILKKIKRLPEQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            12370..13185
                     /gene="thiD"
                     /locus_tag="IW833_001221"
     CDS             12370..13185
                     /gene="thiD"
                     /locus_tag="IW833_001221"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ1307350.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(13514..14965)
                     /gene="cls"
                     /locus_tag="IW833_001222"
     CDS             complement(13514..14965)
                     /gene="cls"
                     /locus_tag="IW833_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1307351.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            15306..17972
                     /locus_tag="IW833_001223"
     CDS             15306..17972
                     /locus_tag="IW833_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1307352.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            18336..19088
                     /locus_tag="IW833_001224"
     CDS             18336..19088
                     /locus_tag="IW833_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1307353.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(19110..20264)
                     /locus_tag="IW833_001225"
     CDS             complement(19110..20264)
                     /locus_tag="IW833_001225"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ1307354.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            20449..22890
                     /locus_tag="IW833_001226"
     CDS             20449..22890
                     /locus_tag="IW833_001226"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ1307355.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(23220..23924)
                     /locus_tag="IW833_001227"
     CDS             complement(23220..23924)
                     /locus_tag="IW833_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307356.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(24111..24356)
                     /locus_tag="IW833_001228"
     CDS             complement(24111..24356)
                     /locus_tag="IW833_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307357.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(24373..25224)
                     /locus_tag="IW833_001229"
     CDS             complement(24373..25224)
                     /locus_tag="IW833_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1307358.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            25390..26394
                     /locus_tag="IW833_001230"
     CDS             25390..26394
                     /locus_tag="IW833_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ1307359.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(26635..26991)
                     /locus_tag="IW833_001231"
     CDS             complement(26635..26991)
                     /locus_tag="IW833_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1307360.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            27359..28546
                     /gene="tuf"
                     /locus_tag="IW833_001232"
     CDS             27359..28546
                     /gene="tuf"
                     /locus_tag="IW833_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1307361.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(28734..31325)
                     /gene="mprF"
                     /locus_tag="IW833_001233"
     CDS             complement(28734..31325)
                     /gene="mprF"
                     /locus_tag="IW833_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1307362.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(31351..32070)
                     /locus_tag="IW833_001234"
     CDS             complement(31351..32070)
                     /locus_tag="IW833_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1307363.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            32390..32911
                     /locus_tag="IW833_001235"
     CDS             32390..32911
                     /locus_tag="IW833_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ1307364.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            32970..33830
                     /locus_tag="IW833_001236"
     CDS             32970..33830
                     /locus_tag="IW833_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ1307365.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            33937..36330
                     /locus_tag="IW833_001237"
     CDS             33937..36330
                     /locus_tag="IW833_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1307366.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            36512..38353
                     /locus_tag="IW833_001238"
     CDS             36512..38353
                     /locus_tag="IW833_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ1307367.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            38377..39486
                     /gene="rpoD"
                     /locus_tag="IW833_001239"
     CDS             38377..39486
                     /gene="rpoD"
                     /locus_tag="IW833_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ1307368.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT        12748 a         6961 c         8186 g        11655 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaagaaaa
      541 aatgaaaaat cggttaattg tcgcatgtgg aagtggagta gcaacgagtc agacaatcgc
      601 tagtaagatc caaagtatgt tagaagatga cggcatcgca tttcctgtag aagcagttga
      661 ttacaaatcg attcaaaatg agcttctaac tgctggaatc tatgtgtatg tcgcacaacc
      721 tgatgaggaa gtacttgaac aagcaaaaga tttgggtatt gaagtatttc caggaattcc
      781 atttttaact ggaatgggtg tagaacctat ttatgacagt atcaaagagt tagtacagta
      841 aaggaggatt gttggataaa aaagttggtt catagtttta tttactatat ttagaaagaa
      901 agaaggttta tttatggaag ttttccaaac cattatcaat tatgttttga acttaggatc
      961 agccattttt gttcccctga ttattttgat acttggattg ttggcaggga tgaaattaaa
     1021 aaaagcattc atgtctgctt tgactttagg gattgctttt agcggaatgt ctatggtcat
     1081 tggctatatg tctaacgcag taagtccagc atcagaagca ttggctaaaa atacgggaat
     1141 cagtttgcct gcacttgatc ttgggtggac aggtgcagca agcatcacat ggtcgtggag
     1201 ttatgctttt atattttttg cagtaacgat tggggtcaat tttgtactgt tactttttaa
     1261 ttggacgaag acgttaaatg ttgatatgtg gaatgtttgg gggaaagctt taactgctta
     1321 tttagtttat tatgtttccg gtagtttggc tgcaggcttt cttactgcaa tggtccaagt
     1381 tatattggaa ttaaagcttg gtgatatgtt tcaaaaacat atacaagatc tcacagggat
     1441 tccattagta acagtgactc attttatgac atctgccgct gtcttgttgc tgcctttcaa
     1501 tatgatcatg gataaaattc ctgctttaaa taaacgagcg gatacgaatg cattgaagaa
     1561 gaaaattggg atcttctctg aaaactctgt aatgggattc attattggat tggggcttgg
     1621 ctttgctgct gcgtatggcg tttcaggttc tcttaatcta gcgatccaaa cagctacagc
     1681 attaagtttg tttcctatga tctctaaaat gttcatgcaa tctttgtctc ctctagcaga
     1741 cgctatgtca gaatttatga aaaagcggtt taaggatcgt gaagtatata tcggattgga
     1801 ctggccgata ttagctggtc gttcagaaat ttgggttact gctattttac ttgttcctgt
     1861 gtttattggt tatgccatta ttttgccagg aaaccaagta ttgccattag cagggattat
     1921 caactattcg attgcggtcg gcggattact tttaacagga ggaaacttgg ctcgtatgct
     1981 gatcttggga atcatcacta tgcctattta tttgtacggg gccacttacc ttgcgccaat
     2041 actcagtgat ttagcagaaa aaagcggagc agtcgaggga atcaagaaag gacagttgat
     2101 cacatggtca tccatagaag ggccggagtt cagggtttta tttgcggagg catttaatag
     2161 gaatattctc gctattgtag gatgcgtgat ttttctagga ctgtttgttc tactttataa
     2221 atatatgata aaagcgaaag taccgagtga acgttatgct gaactacaaa aatagcattg
     2281 aaatggtggg aaagtaatga aaagagaaga gacagataaa ataaaatgga cagtcgctct
     2341 ttgtggtaca cttctgctat ttctatatgg actctttact cagaatatca tcatcaatct
     2401 attggtcatt tttttcgcac tagtgatcta taaatatgga aatcacgttt tattcagaga
     2461 gtacgatgaa aaaagaaaac ggaaaataga agaatcaatg aaaataaaag aagctaccaa
     2521 agaaatattg agagaaaaaa gttttataaa aagataatgg aggggtcatt atgtcagatg
     2581 gacgtatgtt atttgagaga gaacgacagg acatggcacg tatcgtaaga gtgattttcg
     2641 aaaggaaaaa cacaaatgtc gcaggaggga atttttcctt taaaaccact gatgatcaag
     2701 ggaaagacta catcatcatg acgccgacca tgatgtcaga agcctatcta ggagagattt
     2761 ctcctgcaca ggttctagta gtagaaccaa gaacgcgtag aatcgttgac ggagtcggtc
     2821 atttaactcg tgaaatcaat atgcatgaag cgatgtatga gactaatcca gctatcaaag
     2881 ctgtcctaca tgctcatgcg cctaatgcaa tgttctgggc gactactggg ttgcctatgc
     2941 ctaatttgac agaagccaca gctaaagtaa aagagattcc tgtcatggac tttgagccta
     3001 attgctctga agaacttgct gaattgacaa gcgactatgt aaaaaaacag catctcgaat
     3061 taccgcatat gttgctgctc aactctcatg gcgtattgat caacacaggt ggagaatcag
     3121 gaatcgaagc tatccataaa gcactgcaaa tccttgatac agttgaatgg aatgcagaaa
     3181 ttgcctacaa gcagacgatt tttcaagcat tagggattac tgacggtttt tattcaaaag
     3241 gtgagaaaat cgggggattg gaagagttga aacataaaac ggctatttat aataaatctg
     3301 ttattgcagc tggtggagac taacctatct gacacaaaaa actacgactt tcacaaaatc
     3361 tgatccagat cgatatctgc ttcaggttgt attatgtaag tcgtagttct tttcttttat
     3421 ttagttaaga ggtggtctgt ccattccctc gcttgcttga tttcgtccat cgtcaactga
     3481 tgacctgtta cagttagctg aatagtcaca tttgcttggc gtgtttcgaa ttgatcggcc
     3541 aattctttaa aggaagcagg ggaaacgatt ggatcgttat caccataaga aatccagacg
     3601 gttttatcct ctaatgtgga acggctttga tgaacaccta gagacatcgg atggaaaaac
     3661 aaccctttat ttagctcggt ctcacgctcc aataataaat gagcagcgat gtttgcgcca
     3721 tttgaatagc cgacaagcag ccagtcactt aacggtatca gttctttttc gctgatttct
     3781 ttgatttttt catggagttt atctgtttct ttttccaaac tgtccaggtc gaattgattc
     3841 aatccattac gcttgaaaaa acgattcatc ccatcttctt ggaccgtgcc acgaaaagaa
     3901 agcaatgtac tgtttggtgc aagaaagtga gcgatatcca gtaaagaatg ttcatcgcca
     3961 cctgtgccat gaagtaagat caaatgcttg ccacttgttc ctttttcaaa aatgtaggtc
     4021 attttgttcc ctcttttctg tttctataga tgcaaatgaa gtagcatgaa gagattattc
     4081 acgtttttta tttgcatcac ttgtatcgaa aggacggaca taagcttcaa tggcttcacg
     4141 acgttcttct aagaatggag gaagagatag tttttctcct gctgattctg ctggttcatc
     4201 ttcaaggaat ccaggaccat cagttgctaa ttcaaacagt acgtgatgag cggctaggaa
     4261 gtactcagat tggaaataga aacgatcgac gaaaccagaa tttggcaatt caatcttatt
     4321 gattacttga atccagtatt ccaatgcttc tttgtctgct acacgcaatg ctaagtgatg
     4381 gatatttcca aatccttcga ttgcttttgg caagtctgtc cgatgttcaa taatgatgct
     4441 tgcaccattt cccccttcac cgatttcaaa taagtagaaa tctcctgaat ggtctgtttc
     4501 tttgaatcct aagatttttt caagaatcaa gcggatatgc tctaattgac tgacacggac
     4561 aaagacaggt ccaagtcctg tgatcgcaaa ctctgtcggt acattgctgt ttttccaagg
     4621 aatgccagct gctacacctt gattgttttc gtcagagatc aattgataat actgattatc
     4681 gaaatcttca aattcgatat attttttgcc gaaacgttca ttgattgctg aatgagtaac
     4741 gtcatatttt tcaaaacgtt ccaaccaaaa gtctaatgct gcgtcacttg gcacacggaa
     4801 agaagcacga gagatcgtat ctgttccttt ttttcctttt ggctgatttg gaaaatcaaa
     4861 gaaagtcata tctgttcctg gcgcaccagt atcgtctgta aagtaaagat ggtaagtttc
     4921 gatgtcatct tggttaactg tttttttgat taagcgcatt cctaaaatat ctgtaaaaaa
     4981 gcggtagatt tttgtagcgc tagatgtcat tgctgtgacg tggtgaattc ctctgatttg
     5041 tgtatccatg ataaatttcc tcctgttaac ttacaaaaag taagtagttg atttagacat
     5101 agtatagagg gtgcatctct attttgcaat taatttgtct tctaaagtat caggaaggaa
     5161 tcaagaatgg aaaagaggag ggacggatca ataaaataaa aagacttacg actagctgta
     5221 tcgatttcaa aaaaactagc tttaaaaagc taactttaga atcattacgg gtggtcataa
     5281 gtctttttat gctgagttat tttttcattt ttgagctaat gcttaggata gaatagaatg
     5341 ttgatttcac gatctcttct tgttacttga aactgcttac cactgtcacg atctcttggt
     5401 aaacggcttt ttcacaacct ctttggttaa gttgaacgca catccagcac agaatagttc
     5461 cgaacatcaa atcggtttcg gctggaagaa tattcaatcg gctgtccatc tttgagatag
     5521 acgacttgct gaacttccag tacaggatcg ttttctgcac agttcaaata ttgctgatca
     5581 tattcatcac ttttatctgc agaaatagca cgataagccc ctgcaaaatg gacattcaat
     5641 tcttgatgaa gatgatcata gatagaggct aacaaatgtt ctttttttaa acccggcaca
     5701 agatggactg gcataaaggt atgctctaaa acatatggct cgccatctaa gatccttaac
     5761 cggtgaatat tgtagacagg ttgctcggca tcgatcatca atcgttcttg gatatattga
     5821 ttagggaatt caacatcaaa atgaatgatt tgactcttca aagaacgatg tctgcgtttc
     5881 atttggagac tcaaaccttc atactcgtta gcgggcgatg tgtctttttg cataaatgga
     5941 tgattcaaga ttttcgttcc tgatcctcgc tgcgaataga caagtccttc cattgctaag
     6001 atattgatgg cttttttgat tgtcatccgg ctcgcaccaa attcttttac caagtcgact
     6061 tgattaggca aaaaactatc gagaggatac tcttttgaac ggatacgttc tcttaaaata
     6121 tttgcgattt cttcgtattt tggcacgtta ttcacctctt ctagagtata acgaaagatt
     6181 atgcttccga caactcgaag tagcatctgg aacattgttt attcgaaaaa agaggtcatg
     6241 acaagttttt gtcacaaccc cttaatgatc tattaagcaa tatctaaatc tggttgttta
     6301 tctagtactt tcaggaatgg gtaccagata aaggcaacga tgacaaagtc gatgacttgc
     6361 agcataccac caagccatga atttgttgct aagacaccgc ttgcaatgat tggcacagtc
     6421 catggaacag agacgccagt tggtgcaggg acaagtccag cagccattac caaataattg
     6481 aacgtcgtta cgattagagg tgccacaacc caaggaataa ggattgttgc attcaatacg
     6541 atcggtagac cgaagatcgc tggttcattt acattgaaaa taccaggtgc taaagctaat
     6601 cgtccaacat cacgatactg tttcttttta agaacaaagg ccatcaataa aacgacagct
     6661 aaagtcattc cagagccacc aataccaaca gtgaaggttt ccatgaatgg ttttgtgatg
     6721 atgtgtggta attcttttcc atgttgataa gcttctaagt tatcaagcat cagtgtattc
     6781 caaatcgggt ccattacaga gttgacgatg atttgtccgt gaagtccgaa gaaccataag
     6841 aattggacaa agaacaaagc aatcaatgtt gcaggtaaac tacttcccaa accagttaat
     6901 ggtttttgga tcacttcata aacgacgtca tgtaagttag cattgaaaat accagtcatc
     6961 acagcattga ttactaagaa aatagttagt gtcactacag cagggatcaa agcggcaaat
     7021 gattttgtta ctgcaggcgg aactccatca ggcattttga tctgccagcc gcgtttcgtg
     7081 attctgcagt agatttctcc agccaagaaa gcagcgatca ttccaatgaa cattcctttt
     7141 gcgccaagac gatcaagtga aagcacacca gtcacatctt caccgccagc ggttttcatt
     7201 gcaaaaggcg taagaatcaa gaagctggca aaagacacgg caccaccgaa aataccttca
     7261 acgtcataag attttgataa ataatagccg ataccaaaag taacaaaaac cgacatgatg
     7321 ctcattgttg cattttgccc attaccgaat aaattcgata aagttccctt tgttgcgtca
     7381 ctgaaaaaag gcaagttatt gataacgaca acgatcgaac cgaacatcgt taaagggaag
     7441 gaaagcatga aagcatcccg cagcactgta aggtaacgat tttgccctaa taaattagct
     7501 aagggcatga ttttctcaga aagcttgtcc ataaaaccgt tcatttttat tcacctcaaa
     7561 tttatttgca atcgattaca tacaaagtat aacgccaaaa attttaaatg tctagtcatt
     7621 ttcataaaaa gtattgattt attttttagg gttcgttatg attacggtat caagaaaaaa
     7681 gagaggaagt aaaaaaatga aaacctacac attcccagaa aatttttggt ggggttctgc
     7741 tgctagcgga ccgcaaacag aaggaagagt aagtgatgat ggtaaaggag aaaatatttg
     7801 ggatcattgg tatgaattag agcctgaaaa attcttcgat caagtaggac ctgaaaaaac
     7861 ttcgcaagtt tacacaaaat acaaagaaga tgttcagtta atgaaagaaa ctggccataa
     7921 cacatttcgt acatctatcc aatggagtca tttgattcca gaagggacag gtgaaatcaa
     7981 tcaaaaagct gtttcttttt atcgagatta ttttactgaa ttgctggata atggtattga
     8041 accttttgtg aacctttacc atttcgatat gccactacca ctgcaagaaa aaggcggatg
     8101 gttatcaaga gaaacggtcg acgcttatgt ttcttatgca gcagcgtgct ttgaactgtt
     8161 tggagatctg gtcaaaaaat ggtttactca taatgaacca atcgtacctg tagaaggagg
     8221 ctacttgtat caattccact atccaaatca tatcaatatg aaagaagcgg tccaagtagg
     8281 ctatcatgag aatctagcaa gcgcaaaggt aatcaaagcc taccatgaag gcggacatga
     8341 tggtgagatc ggaatcatct tgaatctgac accaagctat ccgagagatg aaacgaaccc
     8401 agaagatgta aaagcggcga agatcgctga cgcattcttc aaccgatcat tccttgaccc
     8461 tgcagtgaaa ggagagtttc cacaagagtt gatcgaaatt gtcaaagaat tggctataat
     8521 gccagagatc catgaaggag acctagaact catccgtgaa aacacgattg acctgctagg
     8581 aatcaattac tatcagcctc gtcgaatcaa agcaaaagaa actccaatca atcacgaaca
     8641 tagccccatg ccagatgatt attttgataa ttacgacatg cccaataaaa aaatgaatcc
     8701 atatcgtgga tgggaaattt acgaaaaagg aatctatgat attttaacga atacaagaga
     8761 aaactatggt aatatcaaat gctatatttc tgaaaatggg atgggggtcg aaggagaaga
     8821 acgatttgtt aacagcgaag gaattattga agatgattac cggatcgagt ttgttacgga
     8881 tcatttgaaa tacgtccatc aagcaatcca agaaggaacc aattgtgtgg gttaccacat
     8941 gtggacatgt atggacaact ggtcttggac aaatgcttat aagaatcgct atggctttat
     9001 cgctgttgat ttggcacaag acggtaaacg gacgatcaaa aaatcaggtc gctggtttaa
     9061 aacggtctct gaaaaaaacg gatttgatgc ctgatatttt ttgataaaac gttcacaaaa
     9121 tgtttgttct aacactgtaa tcgtgctaca ctaacagtga aggaggcgtt tacaatggta
     9181 gatacttatc gtaatatttt agtagcaatt gatggttctg aaaaggcaga aaaagcattt
     9241 cttgaggcaa tcacgattgc caaacgaaat caggcaacgt tgcacatcct gtatgtcaat
     9301 gaagtaacag gcaattactt tggtgatttt gcatttgtta caacaagcct tcaggaagaa
     9361 ttggatgagg ttgcggaaaa ccagatgaaa gaacatcgaa acttggcaat tgaaaaaggc
     9421 ctgacagata tcgagacata tgttctttac ggttatccaa agacactgat tgctaatttc
     9481 catgaatcga aagaaccaat cgatctgatt gtcatggggg cgacaggtct gaatgcggta
     9541 gaacgtgcac tagtaggttc cactacttcc tatgtagtca accacgcatc atgtaacgtc
     9601 atggttgtaa aataaataaa ttaagggaac agaaagcaaa aaagcataac gcttcttttg
     9661 ctttctgttc cttttatttt tttgtaaaaa attactttac ctgatgaagt aatcatgcta
     9721 ttctaattac aacgacagat ggagtaatat ggaggaaaga aaatgatttc aagtgatgga
     9781 atacgcggat acaatgatgc gattatttta tcgatcctta acaaaaacga ttcttatgga
     9841 tatgaaattt ctaaaacgat tcgtgagagg acaaatgatt cctatgtgat caaagagaca
     9901 acactttact ccgtttttgc ccgtttagaa aagaatcaac tgattgtttc gtatcctgga
     9961 gaagtgactc atggaaaaaa gcggacatat tataaaatca ctgaaatagg gcgtaatctt
    10021 ttagaagaaa aaaagcaaga gtggctgacg acgaaagacg tagtcgatca atttttggag
    10081 ggaaattaaa tggatacaat ccgtaattat ttagacagtc tttttatcgg agtacctcaa
    10141 agtaccgaga tagataagct gaaaactgat ttgttggcaa atatggaaga ccattatcat
    10201 gaattgatgg gagaaggaaa aaatgaacag gaggccatcg gtacagtaat ttcgacattt
    10261 ggttcaatcg atgaattatt agaagaacta gatgtcgaaa aaaaacatca agctgacgag
    10321 acggaaacaa acacagcttc tatttattta agcgaagcag aaaactactg gaaagaatac
    10381 agagcggcaa gtctccaagt ggcatcaggt gttctattca ttagcttatc ttttgcaagt
    10441 tttcttttct tttgttcagc aggatatgtg ttcatgggta tttcttgtct gattttcggt
    10501 atagcattag ctgtcggttt ttttattgct tctggtatga aaatcacacg tttaaatcac
    10561 tttttgcacc atcggaaaat acctgaaaaa gtattagcag aagcaaaaga aaaagaagaa
    10621 gagtatcagc gaagttttgg cttctcactg attgcaggaa ttggattatg tattttttca
    10681 cttttcccac ttttggcttc tttaatgtgg tatatggatg gtagtatcgg tgcctctata
    10741 ttttttgtta cggtaggaac gggtgtcttc ttgatcattt atggcagtct agtccgtcat
    10801 tcttatcgtc aattcactca aagcgcttat tactggtgaa tgtattcaga tttgtaaaaa
    10861 tattctattt atccggcgta gaaaaaaaga ctgtgacacg cttttgtcac agtctttttt
    10921 atatataata atttaaaaat tcatcatcta aatccgtaga attttctaga ttgcttaccc
    10981 atgtaaaatc gttatagaca gatttcagtt gacttctgcg ccattgtaca gggataaaaa
    11041 gacgcatgct atcttcattg aatccgacta ataatttctt tccgtcagta atgatgggac
    11101 gttgtaacaa agtaaaatgc gagacgatca gatcgacaag ctctgaggtc gtacattggt
    11161 cgatttggtc tttcaaattt ttataatcaa tcgagcgttt agctaataga tcgtcaaatc
    11221 cgttttctgt taatgacaat aaataaaata tctctttcct tgttaaaggc tgtttaggtg
    11281 tgaaaattcg ttctctgtaa ggcatttgat ggtctttcag ccagtttgcc atcagatggt
    11341 aactatgtgt gcgagaagaa tagatcgtaa gcataaaaaa actccttaaa tagtccatta
    11401 atttgttaga tctgttgaga taaataatag gtaaggagct gtgcataagc attgatcaaa
    11461 acaacccaaa catcattaga aataactttc taaagtctta aacttgcgac gagaaacaac
    11521 tagactaata gaaaaaatgt cttagccatt tggcttcctg tttgtttttt tgcttatgct
    11581 gcagctcctc gggaaagggt tctaacaatt tgaagaaggt tgatgattga tctagtttaa
    11641 attgtgtgaa atttgaaatc tggtgttttt actagatgaa tcacctaaca actatgatta
    11701 taacttatag aacttctgtt ttgcattcgg ttttgttata gttgttataa agttttattt
    11761 tattacagaa catttttaat taataaagaa cagttagaag ttaaaattgc tgattcacat
    11821 agagtttatg acttcttgga aattcttttt caatcagttt tttcatctgt ttatgaaatt
    11881 cttttttttc agtaaacata aaggtttggg tatcgtaacc ttcttcatgt aggaaataga
    11941 ttattccttt ttctgtgttt tgcgtataag cttttatact cctgtctttt ttgaaagaac
    12001 agatcgtata gccatctatt tgctctggta gtcgtttgat tttcttcaaa atgacttttt
    12061 tatcctcaag tttcattttt ttcacctgac tttatttttt tcatatggaa atcatagcgc
    12121 ctttggttat atgaaaacaa gaaggatact gaaatatata taaatcaaat agtggttatt
    12181 tattattaga atagcgaata caaataaacg accactattt tctgatttat tgcaaatgtt
    12241 ttttcaaata aatttgatta ccaagaaaag gtagtggtat atttactgag ggatactttg
    12301 aaaaacatat gatagaaaca gtaacagtat tatgaaaaat ctttttataa aaatggagga
    12361 atcgatttga tggaagatca gtatgtacaa gcaatgacga tcgcaggatc agatagcgat
    12421 ggaagcgcag ggatgcaagc agatatgcat acattcttta caagaaatgt ttacggtgtt
    12481 tctgtcatga ccgcatgtgt tgcaggtaat tcttacggaa tcggagcaag tgtgacattg
    12541 ccaacagatt ttatcgacaa agaatttgaa ctgattgcga aggactttca aatacgtgca
    12601 gctaaaacag gtatgctagc agattcaaag ttgattgaga cagttgtaaa aaattaccaa
    12661 aaatatgatt ttggaccatt agtcgtggat cccgtaatcg taacgaaaca tggaaatctt
    12721 ttactagaag aatctgcttt gcaaagcttg aaagaaaagc ttgtcccttt ggccgaagta
    12781 ctgacaccta atttttatga agcagaaaag ctgactggac ggacattagc agctgaagaa
    12841 gattttgtgc aagcagcaaa agaactgcaa gatatgggtg caaaaaacgt tatgatcaaa
    12901 ggacatcatg ctaaaaatgc gcagcaagaa acagtcaatg actacgtatt gttcgaatca
    12961 ggaaaatcat tttggttaaa tgcaccattt tatcctaccg accggatcaa cggaactggc
    13021 gattcgctat ctgcatgtat tgcagctgag ttagcgaaag gaaaaacagt tgaacaagcg
    13081 atccgattgg caaaagacta tgtaaaccgt gcaattcgtg aagaaataaa tgtagggcac
    13141 aaatatggcc ccatcaatca ttgggcagct ggtgaattag agtaaaagaa agaagttgtg
    13201 aaaaagttgc cctgcatcaa gtaataagaa cagccaagta aaaacagctt cttatgtttt
    13261 taacttggtt ggacttatta ccgcagatgc aagcttttga acaccgttta agaaagaggt
    13321 cgtgagcctg ccgtccagct ccgagtcaca aggaacaagt tcaaaaaaca gcttctcatg
    13381 tttgttgaat attgtgactt gtgaccgagg agttggcagg agaacaccgt ttgttcggaa
    13441 gaagggactg tgacaagatt tttgtcacag tccctttaat cttagtatat ttttctctac
    13501 ttgaataaaa aaattatagg attggagaaa ataatcgaga aatttgttgc ttgaataata
    13561 gccagttaga catttccttg actgtttcag ttgtcatgga tgaacaatgt gtctcatctt
    13621 ctttgaactg agtagcaagt tcttctaaaa actcaggatc gtaaatgaca gcacttgttt
    13681 caaaattcaa tcggtagcta cgaatatctt gatttgctga acctaccatg cagatttcat
    13741 catccattat caatgttttt gcatggagga agccgttctc atagacaagg atttgtacat
    13801 tttccttgat cagctgccga gcataatatt gtgttgctcg ataaataaaa ggatgatccg
    13861 gtttgttggg aatcatgatt ttcacatcta caccagaggc agttgcgatt tttaaagcag
    13921 caatgacact ttcatcagga acaaggtaag gtgtctgtat ccaaacacgt tttttagcag
    13981 aagtaatcaa tttgataaat gaaagcttga tttgttccct ttcgttattc ggtccgcttg
    14041 aaacaagctg gatggatgta tcagcaagct tagattcatc tcgatctgct tttttaaaga
    14101 aataattcaa atgatagccg accttttttt cttcaggtac cgagacgttc cagtctgtta
    14161 agaagcgaag ctggagcaaa gaagaagcgg caccgaaaat ccgtatatgc gtatcgcgcc
    14221 aatagccgaa tttttttgtt gtatttacat attgattggc aatattgaaa ccaccggtat
    14281 aactaatctt tccatcgatc acaacgattt ttcggtgatc atggtaattc aatcggaaac
    14341 gaagtaatgc cctttgtgag gtaacaaatg tatggacatg tccaccattt ttgattagac
    14401 gattgaaatc ttttgctttt gtgccatgag agccaaatgc atcatataat attcgaactt
    14461 ccacgccttc tgcggctttt tcttctaaaa catgtaagac ttgctggccg atattatctg
    14521 tcacaaatgc gtaatattcg atatggatcg aatgtttggc tttttttaga tcttgaagca
    14581 gtgaatccaa tttctcttgt ccgtctgtga gaagagtgac agaattcatt cttgtcagcg
    14641 gcatacgatt taatgaagag aagaagtcaa caaattgctg tttgtcttta tcgcccatgt
    14701 caggatcgta atgttcaatg gtatctcctc taaaagactg aaagttttct aattctttca
    14761 agtcactttg tcggagatag aattttttct tatccgttaa tccacgtccg aaaaataaat
    14821 ataatacaaa gcccacccca ggaagggcaa acaatactaa gagccatgcc caaatggctg
    14881 ctacatctcg gggtttgatc aaaatagcca ccaaagcaat aagtgcattt aatagataaa
    14941 gggcggttat aatactagat accacaaaaa tgttcctcct tacatacaat aacattagta
    15001 tagcatgata gaagaaagaa gcctaccaag gtgaatatac ttttcactcc tttcatgaaa
    15061 atgtaggtaa atcgggctaa agtagttgct ttttgtaggt taataaggta ggatattagt
    15121 acagttgaat atctccttag gggagtaacc ggcaacgttt gttgcgataa tatcaacagt
    15181 aagttaacca aatggttttc tggtattgta tgaaatggtg agacctaaga caaatgcgta
    15241 tttgtcttag gtctcttttt tttcagacag gactagggcc aagatcaaaa aatgggggat
    15301 gaaatttgga aaacaaggaa caagcacaac aagcggtcta taaagaaacg atcgactcgt
    15361 tgatgcagca aacgcagagc tcaaccgatg gattgagcac cacgcaagcg aaggaaaaat
    15421 tagaaagaga tggcttgaac cagctaaaag aaaagccgaa gaagccaact tggaaattgt
    15481 tcttagaaac tttcaaagat gcaatggtta tcgtattgct gattgtcgca ctgattcaga
    15541 tggtcatggg tgactacatc gaattcttag ttatttttgc agtattgatg ttgaattcta
    15601 taatcagtgt tgtccaaaca aaaaaagcag aaggttcttt agaagcgttg aaaaaccttt
    15661 cagcgcccga tgctcgagtg attcgtgatg gtaaggaaaa aacgattccg gcaaacgaac
    15721 ttgtggtagg agatatcgtg ttgttagacg caggcgatta tgtacctgct gatggacgtt
    15781 tactagatgc gggatcatta aaagtagatg aaggtatgct gacaggagag tcgacttctg
    15841 cagaaaaaga agtaacggat atcacaagta cggtaccaat cggagatcgt acaaatatgg
    15901 tattcagtgg aacgatcgta acttacggac gcggtaaatt cttagtgact tcaactggga
    15961 ataatacgga gatcggacaa gttgctggcc ttttggaatc aacaactgag caagtcactc
    16021 cgttacaacg aggtctggat aaattcagta aaaaactaag tttggctatt ttggcattgt
    16081 ctttattgat tttaggtatc caactgtttc gtatttatct tggagaaggt acaggagata
    16141 tgactgcaga tatcgtcagc gcagtgatgt ttgccgtagc cgtagccgta gctgctattc
    16201 cagaagcatt acagtctatc gtaacaatcg tgttatcttt gggaacgaat aaaatggcaa
    16261 aacgtcatgc cattattcgg aagcttccag cagttgaaac acttggttcg acaagtatca
    16321 tctgtacaga taagactggt acattgacac aaaacaaaat gacagttgtt gactactttt
    16381 taggtaatgg cgatactggg gatttcacaa atgacccttc aaaatggaca gctgacgaac
    16441 gtcgcttgat cgaaatcagt gttttagcga atgatgcatc gatcagtgaa gacggtacta
    16501 aattgggcga ccctacggaa gtagcgttta tcgactttag tgaaaaatta aaccagcctt
    16561 atcaggaaat ccgaaacaac tacccaagac aagcagaact tccttttgac tcagatcgaa
    16621 aattaatgtc tactggacac acaattgata acaaaaatta cttgtttgtt aaaggcggac
    16681 cagatatcgt cttccaacgc agtaaaaaag tattgctaaa tggtgaagtc gttccgatga
    16741 cggatgaatt gatgaaaaaa ttccaagagc aaaatgaagc tttttcaaca cgcgcattac
    16801 gtgtactagc ttttgcttac cgtccacttg ataattttga tttatcattt gaagatgaag
    16861 acgatttgat ccttgttggt ttgcttgcta tgatcgatcc tccacgtgct gaagtgactc
    16921 aggcggtaac agaagcaaga agtgcaggaa tcaaaacaat catgattact ggtgaccaca
    16981 aaacaacagc tgttgctatc gcaaaacaaa tcgggatttt tgaagaagga aacctggctt
    17041 tgaccgggac ggaactagat gaattgacag aagatcaact aaacgaagtt ttagaaaaag
    17101 tcactgttta tgcacgtgtt tcacctgaga ataaaattcg tatcgttcgt gcttggcaaa
    17161 ataaaggtca tatctcagct atgacaggtg atggtgtaaa tgacgcacca gcattgaaac
    17221 aagcagacat cggaattgct atgggaacgg gtacagatgt ggcaaaagat gcagcagcga
    17281 tggtattgac ggatgataac tttgcttcta ttgtcagcgc agtagaagta ggacgtaatg
    17341 tgtttgataa tatcaagaaa gctatttctt atctatttgc aggaaattta ggtgcgatca
    17401 ttgcgatcat ctttgcctta gttgctgact ggtccaatcc atttacagca ttacaactgt
    17461 tgttcatcaa tttagtcaat gattcactgc cagcaattgc attaggtaca gaaaaagctg
    17521 aacctgctac gatgaaccgc ccgccaagag atccaaattc aggaatcttt acggacaaaa
    17581 cattgatttc tgtaacgtat cgtggtgtgc tgattgggat tgctgttatc tttgctcaat
    17641 ggcttggcat gcagcattca cctgaattcg gtgtagccat gtcattttca acattgattt
    17701 ggtgtcggac attgcaaaca ttcccagcac gttcaaacac acaaacagct atccaagcag
    17761 ggttcttctc taacagaagt gtcttactag ctgtagttgt atgtagcgcg ctttactgct
    17821 taacaatgat cccaggactg aaagaagtct tctctatccc atcagcattt ggattgacag
    17881 aagtctttac atgtatggga atcgcacttg tgacaattgt tttgatggaa atcactaaat
    17941 tgatcattgt acgagtacaa ggaaataaat aaaacgaatc aacgaggttg tgagcctgcc
    18001 gtttaattct gagtcacaag gtgcaatttc aacaaacagc ttctcatgtt tgttgaatat
    18061 tgtgacttgt gaccgaagag ttggcaggag aacaccgttt aaggaagaag ttgtaaaatc
    18121 agcattttga gctaatactg agggtcatct gattggacac tgtttattcg gaaagaggga
    18181 ctttgacacg actttggtca cagtcccttt ttttatttat gatagacgga aagccaatca
    18241 ttttattatt catcaaaaga gatagaatag ataaaagagg agaatgattt attccgaaaa
    18301 gtgaaaacat ataacaaaaa attagaggaa atcaaatgaa tgaaaaagaa tgggatgaat
    18361 ttgctgaaga atattatgaa atccagcaag aatcgcaagt aactattgct gaagatgtaa
    18421 aaaattatct gaaaacggag aaagtcctgc ctgctgaaac agcagcagac gttgctggag
    18481 gatctggcag atttctttcc ttagcaaaag aaattaatcg ctatgatttg atcgattttt
    18541 cttcaggaat gctagaaaat gcaaaaaaag aagcagaaaa aaatgagttg ccaaatgttt
    18601 cctttatcaa acagtcgtta gaagaattta tccaatcaaa tgagacatat gaaatggttt
    18661 ttacagcagc aaatccagca ttggtacatc ttattcaatt agaaaaattg atgaaaaagt
    18721 caagcaaatg gtgcgtgata atcagagtag tggaatcaat cgatgatgtt ttttatccta
    18781 tcgaaaagca actaggaatg atagaagaag atcctaatac atcgccagtt gtaatggatc
    18841 aatttgaaac atacttaatt gatgaaggat tcaaagtgaa gaaaaaagat tttacttatt
    18901 ttacacagga atcaattact gaagaattga tgaagctata ctatgacgcg tacgcaaaag
    18961 atcctgttgt gaccaggtac attcgcaaac tattcagtga aaaacgagaa atccttagta
    19021 cgactaagct gacctataga ctgctccttt ggtccgcaga taaaaaaaga acctttcacg
    19081 actcgtgaag ggttcttttt atgattttat tatattttgt agtaacgaat atcattttcg
    19141 atctttgtaa tcgaaaaagg tacatctgaa gtatattctg cacattctcc gtgttcagga
    19201 agcgtctcac tgaaaatcgt ttcatattcg tcaacagtga tccgtgttcg agaatctagc
    19261 atctcttgat gagattgtgc gaagagatat tcttgatagt tttcttccag ataacctgta
    19321 aaaaattcac ttactgcacc agaaccgtag ctgaatagac cgatgcgatc tcctggctgt
    19381 agtgatttcg aattttccag tagagaagtt agccccaggt ataatgaacc agtgtaaaga
    19441 ttaccgattc gtcggctgta acggatgctt tcttcatagc gagccataag acgttcctga
    19501 ttttcttcgt ctgtttggtc taatacgctt tgcaatgcct tttttcccat cttagtatac
    19561 ggaatatgga aagcaatcgc ctgataatct tcgagtcctc gacccgacaa ttctttatgt
    19621 cgattccaaa ctttttggaa tgattcgata tacgtagaat tagataaagg accatcaaca
    19681 acaggaaatt cgctataatc tggacgccag aaatcataga tatcttctgt cagaaatacg
    19741 ctgtcgtctt caatcgataa aatacgcggg ttttgagtga tcatcatcgc aacagcaccg
    19801 acaccttgcg tcacttcacc accgcttgcc aagccgtaac gagcaatatc acttgctatg
    19861 actagtactt ttcgttctgg atgatttttg acatattctt tcgccatgtg caaagctgcg
    19921 gtgccaccgt agcaagcctc ttttacttca aaagaacgag caaaagattg gattttcagt
    19981 aaatggtgga taattaccgc actcgctttt gaatgatcaa tcccggattc tgttccgacg
    20041 atgaccatgt cgattagctc acggtctttt tctgttacga ttttgctggc agcatttgct
    20101 cctagtgtaa tgatgtcttc atttgcacga ttgactgcca tttggtcttg accaatccct
    20161 atatggtatt ttgcaggatc atccccacgg ctttctgcca actctgtcat atctaaatat
    20221 aaattaggaa taaaaaagga aagacgatca atccctattt tcatttcaac acctctttac
    20281 ttatgataaa aaacttactt tactaaggta tcataaaaag attaaaagaa atataaaaaa
    20341 gagaatattt aaggattgtt aaaatttaat cttagaaaaa aataactgga acaagagcta
    20401 tttgttttta cagcaaagaa tggtatattt ttgtaggagg tggcttactt gaaagaagtc
    20461 gttatgatag atgctgcaag aacgccgata ggaaagtatc gcggaagttt aagtccattt
    20521 actgcagttg aattaggcac acttgtgact aaaggattat tagataaaac aaaactgaaa
    20581 aaagataaaa tcgatcaagt aatatttgga aatgtcctgc aagctggtaa tggtcaaaat
    20641 gttgccagac agattgcttt gaacagcggt ctccctgtag atgtcccagc aatgacgatc
    20701 aatgaagttt gcggatctgg tatgaaagca gtgatcttag cacgccagct gatccaatta
    20761 ggtgaagcag aacttgtgat agccggcgga acagagagta tgtctcaagc acctatgctg
    20821 aaacagtatc agtcagaaac aaatgaatat ggtgaaccaa tttccagtat ggtcaacgac
    20881 ggattgactg acgcattttc aaatgcacat atgggattaa ccgcagagaa tgttgcaaca
    20941 caattttctg tgagcagaga agagcaggat cgctatgcct tgtcgtccca gttgaaagca
    21001 gcacatgctg tcgaagccgg tgtattttct gaggagatca tcccagtcaa gatttctgat
    21061 gaagacgtgt tatctgagga tgaagcagtt cgtggaaata gtacattgga aaaactgggc
    21121 acgttacgta cagtattctc agaagaagga actgtaacag caggaaatgc ttccccgttg
    21181 aatgacggtg cctctgtggt gatccttgca tccaaagaat acgcagaaaa taataatctg
    21241 ccttatttag caaccatcaa agaagtagcg gaggtcggta ttgacccaag tatcatggga
    21301 atcgctccaa tcaaagccat tcaaaaattg acagatagat caggaatgaa tttatcaaca
    21361 atcgatcttt ttgagatcaa tgaagcattt gcagcttctt ctatcgtagt tagccaagaa
    21421 ctgcagttag atgaagaaaa ggtcaatata tatggaggag caatcgcatt aggccatcca
    21481 attggtgcaa gcggtgcacg tatcttgact acattagctt acggattgct tcgtgaacag
    21541 aagagatacg gtattgcttc attatgtatt ggtggaggtt taggcttggc agtcttgtta
    21601 gaagcgaata tggagcaaac acataaggat gtgcaaaaaa aaaagtttta tcagcttacg
    21661 ccatctgaac ggcgcagtca attaatagaa aaaaatgtat tgacacaaga aactgcgttg
    21721 atttttcagg aacaaacact ttctgaagag ctctccgatc acatgatcga aaaccaagtg
    21781 agtgaagtgg aaatcccaat ggggatcgcg cagaattttc aaatcaatgg caagaaaaaa
    21841 tggattccga tggcaacaga ggaaccatct gtcattgcag ctgcaagtaa tggtgcaaaa
    21901 atctgtggaa atatctgcgc agaaacacct cagcggctga tgcgagggca aatcgttcta
    21961 tcaggaaaat cggagtatca agcagtgatc aatgcagtta atcatcgaaa agaagagctg
    22021 attctttgtg caaatgaaag ttatccttca atcgtgaaac gcgggggcgg tgttcaagat
    22081 atctctactc gggaattcat ggggagtttc catgcatact tatctattga ttttttagtg
    22141 gatgtaaaag atgcaatggg cgctaacatg atcaactcga ttcttgagag tgtggcaaac
    22201 aagttacggg agtggttccc tgaagaagag attctattca gtattttaag taattttgct
    22261 acagaatcat tggcttctgc ctgttgtgaa atcccattcg aacgcttagg gagaaacaaa
    22321 gaaatcggtg aacaaattgc caaaaaaatc caacaggcag gggaatatgc gaagcttgac
    22381 ccttaccgtg cagcaacgca caacaaaggg atcatgaatg gtatagaagc tgtagtggca
    22441 gctacaggaa atgatacacg tgcggttagc gcttcgatcc acgcctacgc ggcaagaaat
    22501 ggattatacc aaggattaac agactggcag atcaaagggg ataaactagt tggaaaacta
    22561 acggtgcctt tggctgttgc cacagtaggc ggagcttcca atatcctgcc aaaagcaaaa
    22621 gcatctttag ctatgttaga tattgattca gccaaggaac tagcacaagt aattgctgca
    22681 gtagggttag ctcaaaacct ggctgctttg cgtgcattag ttacagaagg aatccaaaaa
    22741 ggccacatgg gacttcaagc tcgttcatta gcgatctcaa ttggggctat tggtgaggaa
    22801 atcgagcaag ttgcgaaaaa attacgtgaa gcagaaaaga tgaaccagca gacagctata
    22861 caaatattgg aaaaaatccg ggaaaaataa aaaagcaata tctttagaag aataaagcaa
    22921 gatttacagt tattttattt actgcagatc ttgctttttt ctctatcaga aaatatgttt
    22981 attattagaa ttttttatag ttaaggttag gcatcttaag aaatcaggca actccaagat
    23041 atcttcaggt agaagagaag tcgcctaaca ttgtaggatc ataggtgatt tgttggacag
    23101 gtcaagctga tatttgagcc tgatgttaga aggaatttct ttttacttgc tgtagaaatt
    23161 attatatgac taaaaaggct gggattttaa atcccagcct tttatgacaa atagaatttt
    23221 tatcgatcag taatcgctgt cgctgcaaat tgttcgatca aaggaatcaa agtcaatttg
    23281 actttgtatt tccgttcgag tgctttttcg ataacgaact cattttcagt agtcatagaa
    23341 atagttgcta atttacgagg ttcatcagca tttttttcat taataactac ttcatgttca
    23401 ttttgttctg tgtaaaattt aggatactca gcaacggctt ttgaaaaatc agcaaaactt
    23461 aaagcggcat tcgtatcagt tgagcgtgta ttttcagttg tgtccgcttt tggtttttcc
    23521 gttttttctt ctggcacact gactgtttct tctgtaactg gtgtcgcaac agcttgcgca
    23581 gttggttcat cgtttttcaa tcgttgatca ttccagtaag acacgatttc aataagaaga
    23641 ttgtcacgga atcgttcgaa aatcccgttt tctcctgaac gaattctctt taatacagta
    23701 ttgaagttgt catcgattgg cacgttgatc cgtgtatctt catttgtttt cccagctaat
    23761 ccgatcgtta agatttgacg gtttttggga tcaagcatga cttgtaacgg caaaccataa
    23821 cgtcgcaagc tctcttcttc tagagaacgg aataattgtc ctcttacacc atccaatgaa
    23881 ggacggaatt gatttaataa tttttgtttc tgttttttct tcatatgttc tctccttcgc
    23941 tcataccctt tcgattataa gaaaaaaaca gccaaatgaa aaggagtaat gcaaaaaaac
    24001 ttgcgtatac gcaaattaca acagaaaaat ttgcgtattt tcccgttttt tgaaagaaaa
    24061 ataaaaaatg gaacggatta tgtatatata cataatccgt tccattcttt ctattttttc
    24121 ggtatcaagg caaaaacagg tagttcaggt gaataaatga cttcatgttc gtgccagggt
    24181 ttgaccatta gcgaatgatc gaaccgtttt ttcagttctt cgatgatttg atcatgtgtc
    24241 cagtttcttg caggaaaatg ttggattttc tctgcatcga tcaaaaagac aaaatcggaa
    24301 tgaaaagtct gggtaacttc ttccgagaga gaattcaagc gttcgattgg atccataaaa
    24361 tcacctttcg acttaaaatt gggtagtatc tgggtctttt gctttgccga ctacaccatc
    24421 taactgatcg atttgtttca tttcttcttc tgttaattca aaatcaaata attcagtatt
    24481 ttctttgatt ctgcttggtg tcactgactt aggaagagga aggaattcgt gttgcagcga
    24541 ccaacgcaaa gcaacttgtg caatcgtctt gtcatgtgct tcagcgattt gtttcatttc
    24601 aggcacatcg aagattttac cagtgcctaa tggactatat gcttcgagga tcatatcatg
    24661 tttttttgca tactcaacta cagttgtctg taattcgcct ggtgcaagga aaacttgatt
    24721 gaccatcggc ataatttttg ctgtttttgc tagtgtatct agatggtggg gtaagaaatt
    24781 gctcacaccg attgctttga ttttacctgc ttcatacagt tcttcaaatg ctcgccatgt
    24841 atctgcattt gcttgttccc aattatcacg aaaagcaaca gggtttggcc agtggatcaa
    24901 aaacagatct aaataatctg tttggagctt cttcaatgat tcttcaaaag aagacatcac
    24961 aagttcatag ctgtggtttg cattccataa ttttgttgtc aagaatatct cttctctagg
    25021 aattccactt tccttaatcg cttggccgac gctttcttca ttcttgtatc cttgggcagt
    25081 atcgatatgg cgataacctg cagccagcgc ttctttaaca gaggaaacag cgacatcccc
    25141 atcaggtgtt tgccatgtgc cgaatcctac taccgggatt tcgtaaccat ttgatagacg
    25201 ataagtgctt gttaatgaat tcatactttc agctcctttt ttattcgttc aactataagt
    25261 gtagcgcatt tttcgaaaaa aagaatccat tttgtttcta ctcttgaaat ttttgtttga
    25321 aagaaaatga ttttgattct ggtatttttg attttttttc cgtaaactaa gacagaaggg
    25381 aatgagaaaa tggatatcta tttaaaaaaa gcaattctgc atattattga cagagaaact
    25441 ggttctccag tcttttcaca aaaagagctg gatctgacaa aagaatatat tcgtgatttt
    25501 cttcaaaaga agatccaaaa aatctcttct gctcaaacaa aaactggaca actagcagaa
    25561 gaaagcatga tcgttgaatc acttaaaatc gctcaagaaa attttgtgga agcaagtgaa
    25621 gcaatcgttc agcggtggtt cgatatctac caagaaagtg aagaagcacc tagtgcagat
    25681 gtatttgttg tcttgtatga attagataca gtgatgtact tatctttatt gaaagtcaat
    25741 tatcgtgagg cttacactca ctttgttgaa gcagacgaag cagggatcga caaccgcttg
    25801 attttgaatc gagcaattct aggggcgaaa acacaaaaag cagatgaagc attcgctgtg
    25861 aatttgactg attggaccta cgaattaatt gaaaaaaaat atgagttttc tggcaaaaaa
    25921 gagctttatt tttctggaag agtgatcgaa agtgttcctg caccttcttt agaagaaaat
    25981 gtaaaagtga tccaaaaagt agccaagcat cttggaaaaa aatttgagac agaagaattt
    26041 gatatcatgg ctgatttgaa agaagctgtc tatgatacga tagaagaaaa aggtcggttg
    26101 gatcatgaga tgattgcgga aaaagtcttc aaggaaaaca tcacggcaaa aatggctttt
    26161 aaagaagaag tacaagagca aggatttgtc ccagaagcac ctcctgtaaa agaagtacaa
    26221 gaaatttctg agaaaaaatt tggaaaacaa aaattcaagc tatcaaacgg gatcgaactg
    26281 atcgtaccag tagatgtata tcgcaaccca gatttgattg aatttgtgaa taatccggat
    26341 ggtacgatat ccgtgatgat caaaaatgtg gatgaagtat tgaatcgttt ataaaaatga
    26401 ctagaatatt aggtgttcaa gtaccagtga tcagttaaat gaattgtgtc aagagaagga
    26461 tcggaaaaga cgaatataac aaaaaaagag cgtgagacaa aagtaaagaa cacttttgtc
    26521 tcacgctcta aacacgtata aacggcggaa gcagaagcaa ctccttctta tttcttggag
    26581 ttaaacgctt ctgtcccagc ctctttttat ctaataaaat tttaagacca tttactaatt
    26641 atcgattttt tctgccgcat ttttaggcac ttcagaagcc tttatttctt cccagcttaa
    26701 tacaccttta cgaggattga ctttgagttt aagatacgca ttcatcttta atggatgttc
    26761 tctttcttcg cgaagcgtca cctgtttttt ttcaccatct ttattataag ccgcttgctt
    26821 gtaggtataa gtcgtgaatt tctctccgtt atctgcttgg gtagctgaca tttcgccatt
    26881 cgtcgtgatt tttgtataat aagcttcgcc gccgtaaaaa taatgataag ctgcaaaact
    26941 tccgccgaga aatatgccta aaacgacaag tacgccaatt aattttttca tgtacttcac
    27001 ctctttcctt attattaacg aaatagaaga tttcgtctat tgaaagttgt tggcaacagc
    27061 tgaattttaa acttttcctc attttgttct tattgcttca aaagaaagtt aaggactatc
    27121 ttgaagtttt tctcaaaaaa cacatttttt tatttataaa aggtactttt ttcgcgtata
    27181 agacagacga gtgctcttag ttgactttga ccaatattga cgtataataa gcttagttcg
    27241 aatcttacaa aaagtaagtc ggacgtattc tttttgattt agtcaaaaaa agactataat
    27301 aaaaaggtga gagctgaaat ttttcagtga tctatattat ttaggaggtt tgtaaattat
    27361 ggcaaaagaa cattatgaca gaagtaaacc acacgttaac attggtacga ttggacacgt
    27421 tgaccatggg aaaactactt taactgctgc gatcactaca gtattaggca aaaaaggcct
    27481 agcaaatcct caagactatg ctagtattga tgcagctcca gaagaacgtg aacgtgggat
    27541 tacgatcaat accgcacacg tagaatatga aacagaaaaa cgccactacg cgcatattga
    27601 tgcaccagga cacgcggact atgtgaaaaa catgattaca ggggcagcac aaatggatgg
    27661 tgcaatctta gttgtttctg caactgacgg tccgatgcct caaacacgtg aacacatttt
    27721 attgtcacgc caagttggtg taaaatacct gattgttttc ttgaacaaag ttgatttagt
    27781 cgatgatgaa gaattgatcg atttggtaga aatggaagtt cgcgagttat tgagcgaata
    27841 tggtttccca ggcgatgaca ctcctgtgat caaaggttcc gcattaaaag cattgcaagg
    27901 cgatccagat gctgaagctg ctattatgga attgatggat acagtagatg aatatatccc
    27961 aacaccagaa cgtgatacag ataaaccatt actattgcca gtggaagacg tcttctcaat
    28021 tacaggtcga ggaactgttg cctcaggtcg tattgatcgt ggtgctgttc gtgtcggtga
    28081 tgaggtagag atcgtaggga tcaaacctga aacacaaaaa gcagttgtaa caggtgtaga
    28141 aatgttccgt aaaacgttag attacgggga agctggggat aacgtaggcg tgttgttacg
    28201 ggggatccaa cgtgacgata tcgaacgtgg acaagtactt gctaaaccag gttccattac
    28261 tccacataca aaattcaaag cagaagtgta cgtgttgaca aaagaagaag gtggacgtca
    28321 tactccattc ttcaacaact atcgtccaca gttctacttc cgcacaactg atgttacagg
    28381 aacaatcaca ttgccagaag atacagaaat ggtcatgcct ggtgacaacg tgacgattga
    28441 tgttgacttg atccatccaa tcgctgttga aaacggaacg acattctcaa ttcgtgaagg
    28501 cggacggaca gtaggttcag gtatcgttac agagatcgaa gcataagaaa cgcgactcga
    28561 atattttggt aatgaaaaat gtttaaaata aaagagcggt ccaaacagga tttctgtttg
    28621 gaccgctctt tgttcttcaa ttgtataaga agagattgta gaagcatgaa ctttcgtttt
    28681 acttcagtct cttttattca tcatgagagt gttcatctat cggtatgcag tttttaatac
    28741 tttcttcgta tccatttttt caaaccactt attctacctt gtgtctgctc aactggcgca
    28801 ttatcaataa tcagcaaagc aatcataaca tatgcaatcc aactatctct agaatataga
    28861 gtataacgtg agacccagtt tgtagcgtac ttgcttttat attctcttaa tccatggaaa
    28921 gaataaaaat gagaaccaaa ctcgtaaacc aagtatgcaa tcctttcctg taaaaaactc
    28981 tttctggaag ttccgacatt tgaaagaggg gccatcccta aattgaacca ttggatatgt
    29041 tcttctttca tataggcaaa caaatgcaag aacaagaaat ccatactgcc agaaggtgct
    29101 tttttagaat catatcgcat cagatcaatc gttcctactt tattgttcgt gtaagtaggc
    29161 atgatcgttg caaaagcgac aatttcttct tgggtatttt tgacaactgc aataggcgct
    29221 ctttgtagat aatcttcaga gaaaaagcct aaagaaaacc ctttttcctt tcgtgaaccc
    29281 aaccaattat cagagatatt tttgaaagta ctcatttgct cagtagaaaa tggtggttgt
    29341 aagacatcaa atgtaaatcc ttctcgttcg atccgattca agacagcacg agtgcccttc
    29401 atttttttcc ctgaagttgt aaaactattc aggtctacat aagcttcttc ccccatctta
    29461 atgaaatcgt agccgaattc atgcaaaatc ataacaatct cttcgcttgt ttcgtagaaa
    29521 actggtaaat agcatagtcg atctgtttct tcgataaaag cttcgattgc ttctggaaaa
    29581 tcttctttct taccagaagg atcacccatt acaatacatt tattattgaa gcaagcgaat
    29641 tggagaagaa cagttggttc accgtctttt tcataggcaa acatccgctt atcttttaag
    29701 aagatcaatt gactatcgga gtttcctcca taagttgtaa ggatcttcag ggccttttct
    29761 tcattaaagg cttcgccaat ctgtttcttt tctccttgaa ggaaatgaac aaatagaacg
    29821 atcatgaaac taacagcgat gatggccaac aatcccgaaa accaaatctt ttcagaagga
    29881 aataagaaaa aggaaataaa atggtgacgc cgatgaggga aatccggcag attgtatacc
    29941 ccaataacga tgtaaagcaa ggacagtgca ccaataatga ttccatcgat cgtacgccac
    30001 tcccatgaat agatcaattg ttcccgaaat aattcgtttt tagaagcaag aatgatcaga
    30061 agcagaatag acaagaaaac caccggtgtg aaactaaaat cacttaataa aacataaagt
    30121 aaagctagaa caatcaagaa gattgtaggc aaatatgctc gtttcacacg tgctgcgatc
    30181 cctctgccca taatgattaa aagaaatccc aatagtatac taggaaattg gataataaaa
    30241 tgaaatttca atgggttcaa actatgaagc cagcgaaact ctgtaaaagc ttgcgggata
    30301 gtggcagaaa gtactagcat gattccagaa aagtataata agacaaccac gattttatgc
    30361 gcaatttctg ttgctaattg tttagggata ccagaataac gctgatcaaa agtggaaccg
    30421 atatttttga agaaaaagac gattccaatc aaaaacggaa tgatgtagta gaacaggcga
    30481 tagagcaaga tccacacaac gactatttct cttggcacac caatagcgga caacccgata
    30541 atcatcatta catcgaaact cccaatttct cctggaatca tagacacgat tcctatcact
    30601 gaagcagcaa tgaataatgg aattgtttgc cacagatcaa tccggatttc cattaaataa
    30661 ccaacagaaa ggaaactaaa caggacaccg gtccattcca acaatgaaac tagcaatagc
    30721 tgtccttgag ttttaagtga ggtaccacca aggaatcctt tctttttgaa agtagtaaat
    30781 aaaaagacga caggaaaata tagaccgccg ccgatcaacc atatccaata ttgcttcaaa
    30841 aaaggagcaa ctggcgtgaa gactacaagt aaaaaagaaa gcagactgta aatagaaagt
    30901 ccagccatca aaaacaagaa aactttcgat aatgcttgga tcacgtttct gctctctttc
    30961 ttttgtccat atagttcaga gcgaagcccc acactaacaa acccgccgaa accggcaata
    31021 ttgttgatcg tattgatcag ccagcttgtc tcaaacaggt aacgaggatt ctgcttttgt
    31081 tcaagcatcc gattcaaaat aatatcataa ccaaccatag gtaacacaga gataagacca
    31141 atcaatagca ttagtccgct tttccataca ggtatagtgg caaacgtttc tcctaattgc
    31201 tggatagata gagttttacc gatggacatc aattcaccga caataatgac aatgacagaa
    31261 atcaaaaaga ttgttttgaa cagtcctagg tggtttttca gccattgtat ggtatttttt
    31321 aacaaataaa tcaccttcta ttcctaatag ttatgtttca gaaagaaatt gaagaacttc
    31381 tcgattgaag cgttcaggat cttttctagc taattcatgt ccttgttctt tcacaagcac
    31441 aaaggaagct tttggtatag ttttagctat atacaaagaa tgttttagct tgattacatc
    31501 tttttttccc acgatgatca aagtggggga gttgattttt tttaaatcat tttctgtaag
    31561 cccgatgtca tgcagcaaaa gtttgataac taaaagattt ttgcgaagac ttgggcgaaa
    31621 taaagagagt aaccataccc atgcataatg aatattactg atgactctgg cagaaaaacg
    31681 aacgccttta acaagcgtgt ttccagaatt taaaatcaat cgatgaactt ttttagggaa
    31741 gctgctggca aataccagtg ctagatttgc tccatcacta aatccaagaa aatctgcctg
    31801 atcgattttt tccagcaaca tgatcgtatt cagatcctcg gccattaact gaaaattcaa
    31861 catagatgct tcgtttgtcg aacgaccatg tcctcggcta tctactagat acacggtata
    31921 gtagcgttcg agtacaggga cctgttctga aaaaaagcgg ccgctgccgt cattgccatg
    31981 cagtaaaaag agagggaagc cttgaccgct cttttcatag taaatttcgc tcccatctgg
    32041 catttttgca tatttctttt cttttttcac ataatcacct actgtgtaaa gtataacgat
    32101 tatatcagtc attgggcaat tgtttttact gaaaaacaac gatcattttc gtaagatgtc
    32161 aaaaaataca ggaaaattca ttttttctta aggcgtacac agaaatcaag gaagaaatag
    32221 ccaagaagat aaagaaatag taagataaaa aaagatgtca aaataactag gtgatgaaat
    32281 gaagcgaaaa cgattatcta ttatccgtat tcttttttac atgatcattc tgacggttat
    32341 tggtgcggtg ttgtttttat gaggaagaac ttatcgttcg gtaaaacgag tggaaagcta
    32401 tcaaatagaa ataacaacag ctgcaaaaaa ttatcatatg gaaaactacg agaatttgat
    32461 caaggcgata atattgaccg aatcaaaagg aaaagggaca gatctcatgc agagctcgga
    32521 aagtctgcat ggagaagctg agatcattga taatccagct gacagtattg atcaaggtgt
    32581 caaatatttg gcggaaatga tagtagaagc aaagaaacaa ggatgcgatt tatggacagc
    32641 tgtccaagca tataattttg ggaaagatta tattgcctat gtaaaaaatc atggcggaga
    32701 aaacacagta gaattagcag aagcgtattc taaagatgtg ctgagcccac ttttaggaaa
    32761 tgaggaaggg aaacaatacc gatattggcg gatccagtcc ttgctttaca atggcggcta
    32821 tctctatcat aatggaggta acatgtttta tcctgatatt gtcaaaatga atgaaaaaat
    32881 aatagagacc tatgaaaaag tctttaagta aaatagcagg aaatgttcaa tcatgaataa
    32941 aattaaatga aagaaggagt gcgcaaatag tggaaaaaag gcaaaaaatg gaaaatcaag
    33001 aagtggggta cacaaccaaa gactggatgt tacggtttgt gaagggaatg tttatcggtt
    33061 caggatttat cttgccagga gtcagcggtg gtgcattagc agctgtcttt ggcatgtatg
    33121 agcggctgat ttctttttta gcccacctta caaaaaattt caaagaaaat gttttgtttt
    33181 ttctgcctgt tggtctaggc ggagtgacag gcatctttct actttctttt ttagtcagtt
    33241 ttttattagg tgcgtatgaa acaacgatat tatggttttt tgtcggatgt atcgctggaa
    33301 ctgttcctgc gttatggaaa gaagcaggga aaaaaggaag agatacttct gatgtgatca
    33361 tcatggttgt tacttttgtt ctggcttatt tgtttttatt atacggcgct caattatttg
    33421 acgggcaagt cgatcagaat tttggaacat ggctgattgc cggcggattg attggtttgg
    33481 gtatgatcgt cccaggattg agcccttcta attttttggt gtatatggga ttgtataaag
    33541 cgatggctga cggatttaaa gaagtacgac ttgaagtgat tgtgccgatc ggaattggtg
    33601 ggattctttg tgttcttgga ttgtcaaaaa tcatggatta cattttctca aaggcatata
    33661 gcaagctatt ccatttcatt ttaggtgtag tttttgcttc aacgattatg atcattccta
    33721 ccgattacgc gggattggga gttgctgggg tactagcttg tcctgttctt tttatagccg
    33781 gtacagcact aggttggtgg atgagccgtt tggaggaaca atataagtaa agatagattg
    33841 ataataaaaa cagcacacgg gtgattcaaa caaaaaggtt ggcatctttt ttgtaaaatc
    33901 attcgtgtgt ttttctataa attttataaa aacaagttgt ttttaatctt ggaacacgct
    33961 agacttaaac acgtgattat ttttgaaagt tggcggaaaa tggagaaaaa gaaaatgaat
    34021 ggacttacac agcaagaggt agttcagaaa atggcttctg gccagcaaaa tgactaccag
    34081 gaagatgcat caaaaagtac aaaacaaata ttcagcgata atatattgac gttattcaat
    34141 tttctgaact ttggtattgg gatttgtctg ttatttgtag gtgcgtattc caacatggcg
    34201 tatttagcaa ttattgtcgt caatatcgtt attggaattt atcaagaaat acatgcaaga
    34261 aatcttgtac ggaaattatc gattgtttcc caatcagaag tactagtgat ccgggacggt
    34321 aaagaacaat cgatttctac taaagagctg gtattaggag atttggtaaa aatcggagca
    34381 ggagaacaga taccagccga tttacaggtt atatctggac gggcagaagc aaatgaagct
    34441 cttttgacag gcgaatctga tttgattgtc aaagaaactg gagctgaatt gctttctgga
    34501 agctttttga caagcggttc tgtcatggct gaagttatcc gagtaggtgc agataattat
    34561 gctgtccgct tgactagcga ggcaaagact cacaaagcta ttcgttctga acttgtagat
    34621 tcgattcgca aagtatccaa attcactagt ttcgtgatta ttcctttggg gatcatacta
    34681 tttttggaag cactgttcat ccgcgatgct tctatcaaaa tttctgttat tgcatctgca
    34741 gcagctttgt taggcatgct tccaaagggt ttggtattgc taataagtat tgcattaacg
    34801 acaggggtaa ccaaactggc aaaaaaacga atccttgttc aagacatgta ttcaattgaa
    34861 acgctggccc atgtcgatac attatgcttg gataaaacag ggaccattac tgaagggaaa
    34921 atgagtatcc agcgggtaga agcactacat ccgctgtacg agaaagccat cccagaaatt
    34981 atggggacgt atctggcaga aagcagtgat aacaacatca ctatgcgggc tatgagagat
    35041 tattttgagc tttctaatcg gtatgatgtg acaaacaaag tcgcattttc ttctgatcgc
    35101 aaatggggcg cgatggaatt tccagaactg ggagtcgtgt acgtaggagc gcctgaacgt
    35161 ttggtggaag aagatcggct tccggacagt ctaaaaacag cccaagcgaa tggctatcgc
    35221 gtacttatgt tggggatcgc tccaaatcag caattgaacg aagaaaaacc agcatttgtt
    35281 gagccggttg ctctgtttga aatcgatgat ccaatccgaa aaaacgctca agaaacacta
    35341 gcttatcttc atgaggaagg tgtggatctg aaagtgattt ctggagacaa tccttttact
    35401 gtctccaata tcgctagacg tgcaggatta ccgaattatg atgcgtatgt cgatctttct
    35461 caaattactg aagaaatgga agtgcgagaa gctgctcatc gctatacggt ttttggacga
    35521 gtgagtccgc agcaaaagaa attgctggta aatgaattga aagaaagcgg cagaacggtt
    35581 gcaatgacag gcgacggtgt gaatgatgtt ttggcgttaa gagaagcaga ttgcagcatt
    35641 gctatggcag aaggtgatgg agcaacacgt cagattgcga atctggtatt gttggactct
    35701 gattttacta cattgccaga agtcctgttt gaaggacgcc gagtggtcaa caatgtgacg
    35761 aaagtctcag gcatcttttt tatcaaaacg atttattcat ttattttatc gattatatgt
    35821 gccgttacag caattggatt tccgtttatc cctatccaaa tcacgctgat tgatttagcg
    35881 attgaagggt atccttcctt ctttttatct tttgaacaag ataaaagaaa aatcacttac
    35941 cgttatttgc ctacagcact tattaatgct ttaccaaatg ccttgcttgt agtagtgaat
    36001 atcattgcgg tgtatctact tggaaaaaca ttcgcatggg gaaatgctga aactactact
    36061 ttgatgtatt acttgctgat tgggatcagt tgcatggcag ttgtaaaagc ttgttatccc
    36121 ttcaacccat tgcgcatctt cctagcagta acgacgatcg tagggatata tgtagctgca
    36181 atgctgttcc atggaatttt ggaagtaaac ttagggttgg gagagagcgt aggatacttc
    36241 tttgtattca tgctcgtaaa tattgttctt cgagtattgt actggaaggc tgatattcaa
    36301 aatcagttac tgaaaagagt agcaaattga tgcttaatga gatagaaatg tgttatactt
    36361 gaaaaagcaa tgaaatatga tacaatcaaa catgtataat tgtgtccttt aaaatcgcac
    36421 tgatttcagt tagtgcgatt ttttaccggt tattctcctg aaatatcttc tgttaaaccc
    36481 aaaagatatc aatagagcag ggggtgaaaa aatggctcaa cggattcctc aagaggttat
    36541 cgaaacagta cgcagtcaaa cgaatatcgt tgaggtgatt ggacaatatg tccagttgaa
    36601 aaagtctggt aaaaattact tggggctgtg tccttttcat gaagaacgta caccgtcttt
    36661 ttctgttgca gaagacaagc agatttttca ttgctttggt tgtggtaaag gcggaaacgt
    36721 gtttactttt cttcaagaac tagaaggtct aagttttcca gaagcagtgg taaaagtggc
    36781 agacttcgaa cagataccgt tagaagatca atggcgtcag cgtactatag ctgtccaaaa
    36841 tcctgaatct gctacgggga aactgattgc tgctcatgaa aaagcagcgg aaatctatca
    36901 tcacatgcta ttgcatacaa aagctggaca gccagcttta gaatatttgc aaagtcgtgg
    36961 gttgtcttta gaattaattg aagaattcaa cataggtttt gcacctaatg agcgttcttt
    37021 tcttcagcaa gtattcaaga atgaatcaat ttcaaatgaa ttgttagaac gctctggatt
    37081 gtttgtcacg catgatgatg gccgtatgtc tgatcgtttt tatcaaagag tgatgtttcc
    37141 acttcgtaat tctcaaggcc agacgatcgg tttttcagga cgctggcttg cacctgaaaa
    37201 agggcaggaa aaagatcagc ccaaatactt gaatagtcca gaaacagaac tgttcaataa
    37261 aagacaagtt ttatttaatc ttgataaagc aagaagcgat atccggaaaa acggagaaat
    37321 tttgttgttc gaagggttta tggatgtgat tgctgcatgg caggctggta tcaaaactgg
    37381 attagcttct atggggacaa gtctaacagc tcaacagatc caacaaatgg aaaagctgac
    37441 gaaagaattg gtcttttgct atgacggaga caacgcaggt tttgaagcaa ccaatcgagg
    37501 gattgaattg cttcggcaaa acagtcgatt agcactttct gtcgtagtcg ttccagaaaa
    37561 gcttgatcct gatgaatatc tccgtaagta tggagaagca tctttccaag aattagtggc
    37621 tcatgggcgc gaaacagtct ttacttttaa atcacgttat ttaaaacaag ggaaaaattt
    37681 agataatgaa aaagacaaga tcgaatacct cgaagaatta ctagttgaac ttagccgagt
    37741 tgtttcacca atcgaacaag atatgtatct atctcaactg tcaacagagt ttcagatcgg
    37801 cagagagacg atacaaaaac aactgcgcga tcttcgaagg acaaatcagc aaaatcgtca
    37861 agaacctcag gaaagtcaac acactcaggt tacgagacaa gaaactgcac ggaaaaagag
    37921 acctttgacg caagtagaaa aggcagagca gattctatta tatcgtgcat ttaaagaagt
    37981 ttctgtccgt aatattttaa aagaacgaga atttcagttt attcatgatg catatgcaga
    38041 agtttatttt ttattcgatg catatgtctc acaacattat gaattcaacc ttgcagagtt
    38101 tttagacttt ttgcaagatg aaaatttaag gcggttggtg gtggaaatcg aatatttacc
    38161 ggtggcagaa gaaagttcgc cacgagaaat ccaagacttg ctagatgtga tcagcaaatc
    38221 tggcttagct gatgaaataa cattaaaaaa acaaatgcag caagaagcca gacgaactgg
    38281 aaacaaacag cttgagcttg aattgacgat tgaaatcatc aatttaacga aacagttaaa
    38341 acaagccaaa tagctactca aggggggcct tcttttatgg caaatggaac agataaaaaa
    38401 acgtatgaag cagcagtagc agctttcatc aaagaaaaca aacccaaacg acaagttgta
    38461 tatgatgatt tatccaatca gcttgctaca ccattcactt tgaatgcaga tgaaatggac
    38521 aaattgatcc aaaagattga agacgcaggt atcagtgtgg tggatgaaaa tggtgatcca
    38581 tcaattcaca gcttgaagag tgctgagaaa aaagctgaac aagcaaaaac agaagatctt
    38641 tctgcgccaa ccggtgtaaa aatcaatgac ccggttcgta tgtatctgaa agaaattggc
    38701 cgtgtctccc ttttaactgc ggaagaagaa gtagcgttag cattgaaaat cgaagaaggc
    38761 gaccaagaag cgaaacagcg tctggcagaa gcaaaccttc gtttagtagt aagtatcgct
    38821 aaacgttatg taggacgagg catgcagttc cttgacttga tccaagaagg aaacatggga
    38881 ctgatgaagg cagttgaaaa atttgactac cgcaaaggat ttaagttttc aacctatgct
    38941 acatggtgga ttcgtcaagc catcactcga gcaatcgctg accaagcacg tacgatccgt
    39001 attccagttc atatggttga aacaattaat aaattgatac gtatccaacg tcaattgctg
    39061 caagaccttg gaagagaacc tacgccagaa gaaatcggtg cggaaatgga tcttccaaca
    39121 gaaaaagtcc gagaaatctt gaaaatcgcg caagaacctg tttcccttga aacgccaatc
    39181 ggtgaagaag atgactctca cttaggtgat ttcatagaag accaggaagc aacaagtcca
    39241 gcagaacacg cagcttatga gttactaaaa gaacaattgg aagatgtact agatacattg
    39301 actgatcggg aagagaatgt ccttcgctta cgttttggtt tagatgatgg ccggacgaga
    39361 acattggaag aagtagggaa agtatttggt gttactcggg aacgtattcg tcagatcgag
    39421 gctaaagcat taagaaaatt gcgccatcct tctcgttcaa aacaattaaa agatttctta
    39481 gaataagcgt ggactattga ccagaagaga aatcagtaaa actgtttggt ttattttaaa
    39541 agaaaaaaag
//
LOCUS       DACEQH010000023        39110 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.023, whole genome shotgun sequence.
ACCESSION   DACEQH010000023 DACEQH010000000
VERSION     DACEQH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.023"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(55..402)
                     /locus_tag="IW833_001240"
     CDS             complement(55..402)
                     /locus_tag="IW833_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307369.1"
                     /translation="MFKVYFPFQWLFKGILLLLLGLILFGSYLTIKARYFPEEKPVKI
                     VTKDLKMGETYQADGVEITFKKVELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENKRLFVKSCW"
     gene            complement(395..697)
                     /locus_tag="IW833_001241"
     CDS             complement(395..697)
                     /locus_tag="IW833_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307370.1"
                     /translation="MSDPYIKVYCSKETKTIFDSFYEQNKGIFGTKANMFRVMVSNLP
                     VLANQSNVKLSNPESKGIEQKISEIESIVVGKLDEIDQKLSYYLKNKCKTEEGKDV"
     gene            complement(913..2304)
                     /locus_tag="IW833_001242"
     CDS             complement(913..2304)
                     /locus_tag="IW833_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1307371.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDNSEE
                     ALQEMNLVRTRMNKSEDIAQAFHVIHSFDKNISKELSIEKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNIDMETCKRLRIDPKDLERMRKINDEVLVENGLEPVDNQY
                     YENLEKRTNLYSNDPSKLKNGKINYQEFVQNAIEATLENCEVNSFVRFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQDVNFYERRDELITLGREKQSRKEKSQYVERTFSARKLGK
                     KYSFESVFERLEKNLEETHMNNFVDLDTEQIIALASGVDYKTGELNVPFVELDSIAQK
                     QFSDHEGAKKIIKYDDFLREVDRHYANTISLEDDQEYTIKLHTDVNPRTLRSTKSKTI
                     LVKKTDQKELFLPAEGFKNCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQRRQRDFEMDF"
     gene            complement(2289..2900)
                     /locus_tag="IW833_001243"
     CDS             complement(2289..2900)
                     /locus_tag="IW833_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ1307372.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVALIKQLNQIGNNLNQAVKALNIIAKYFREQSENKKEK
                     SLHFSFDVSRYNIFKDELANSIAKKMMISYPDDIFLESNRSTSSFLKLSEVEKDYFQY
                     WLEKQTRPDTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(2912..3100)
                     /locus_tag="IW833_001244"
     CDS             complement(2912..3100)
                     /locus_tag="IW833_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307373.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     MMKNEIESNFKCCSLEKM"
     gene            complement(3114..5651)
                     /locus_tag="IW833_001245"
     CDS             complement(3114..5651)
                     /locus_tag="IW833_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307374.1"
                     /translation="MKIIKKKIANRKTNWLPVIFLVLIAVILFTSNIAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESTGLNKVYQAVLSTIVVGLMILSLVIIGYKMITGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVEGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLGTGSGSSVNPFIKAVA
                     YVCAVIVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTVYAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKS
                     AEVLINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKSVKENLQNSTSSQDRRSVDVRENLQNTTSSQNGNRTVYVRENVQSSGTSQPRTVDV
                     RENLQNTTSSQNGNRTVDVRENVQSSASNQPRTVEVRETRAEASQGDKTQKINIIEER
                     KERPNEKVIVEQEVKEKKRPRFTYENNPLFQDISDKPNPLFDSLFKK"
     gene            complement(5638..5856)
                     /locus_tag="IW833_001246"
     CDS             complement(5638..5856)
                     /locus_tag="IW833_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307375.1"
                     /translation="MIENHLVDKIHCLDQNADTLELTESICSNTIVLDLKAVNDDYVT
                     HIIIDRKIAQKLSESLRKWAEGGNNENN"
     gene            complement(5858..6901)
                     /locus_tag="IW833_001247"
     CDS             complement(5858..6901)
                     /locus_tag="IW833_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1307376.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKNKMQMFPNFYYLLKPVLCKDDVYKINKITHQENSQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLFESLS
                     TKNNKLDEELIETFIEENLLKQMWGESIVNCLKLASNSDYRQFDNWYKKFNYAIKSAE
                     KEQKVQLKIIYEICNNSYFVDHVREQLAMTLRDLIRRAKTDHRIKQKNNYIFTSLKNK
                     ALELIKLQKKEGI"
     gene            complement(7056..7382)
                     /locus_tag="IW833_001248"
     CDS             complement(7056..7382)
                     /locus_tag="IW833_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307377.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(7390..7593)
                     /locus_tag="IW833_001249"
     CDS             complement(7390..7593)
                     /locus_tag="IW833_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1307378.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKSK"
     gene            7668..8318
                     /locus_tag="IW833_001250"
     CDS             7668..8318
                     /locus_tag="IW833_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1307379.1"
                     /translation="MSIKTLLNLWFLCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            8373..9539
                     /locus_tag="IW833_001251"
     CDS             8373..9539
                     /locus_tag="IW833_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1307380.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(9628..10020)
                     /gene="rpsI"
                     /locus_tag="IW833_001252"
     CDS             complement(9628..10020)
                     /gene="rpsI"
                     /locus_tag="IW833_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1307381.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(10036..10479)
                     /gene="rplM"
                     /locus_tag="IW833_001253"
     CDS             complement(10036..10479)
                     /gene="rplM"
                     /locus_tag="IW833_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ1307382.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(10998..11465)
                     /locus_tag="IW833_001254"
     CDS             complement(10998..11465)
                     /locus_tag="IW833_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ1307383.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(11585..13123)
                     /locus_tag="IW833_001255"
     CDS             complement(11585..13123)
                     /locus_tag="IW833_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ1307384.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            13203..13889
                     /locus_tag="IW833_001256"
     CDS             13203..13889
                     /locus_tag="IW833_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ1307385.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(14090..14617)
                     /gene="msrA"
                     /locus_tag="IW833_001257"
     CDS             complement(14090..14617)
                     /gene="msrA"
                     /locus_tag="IW833_001257"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1307386.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14701..15378)
                     /gene="pgmB"
                     /locus_tag="IW833_001258"
     CDS             complement(14701..15378)
                     /gene="pgmB"
                     /locus_tag="IW833_001258"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1307387.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(15375..16505)
                     /locus_tag="IW833_001259"
     CDS             complement(15375..16505)
                     /locus_tag="IW833_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1307388.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(16505..18688)
                     /locus_tag="IW833_001260"
     CDS             complement(16505..18688)
                     /locus_tag="IW833_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1307389.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(18685..19710)
                     /locus_tag="IW833_001261"
     CDS             complement(18685..19710)
                     /locus_tag="IW833_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1307390.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(19755..20582)
                     /locus_tag="IW833_001262"
     CDS             complement(19755..20582)
                     /locus_tag="IW833_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1307391.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(20579..21646)
                     /locus_tag="IW833_001263"
     CDS             complement(20579..21646)
                     /locus_tag="IW833_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ1307392.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(21672..22508)
                     /locus_tag="IW833_001264"
     CDS             complement(21672..22508)
                     /locus_tag="IW833_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1307393.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(22505..23374)
                     /locus_tag="IW833_001265"
     CDS             complement(22505..23374)
                     /locus_tag="IW833_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1307394.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(23444..24727)
                     /locus_tag="IW833_001266"
     CDS             complement(23444..24727)
                     /locus_tag="IW833_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1307395.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(24741..26426)
                     /locus_tag="IW833_001267"
     CDS             complement(24741..26426)
                     /locus_tag="IW833_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ1307396.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            26701..27726
                     /locus_tag="IW833_001268"
     CDS             26701..27726
                     /locus_tag="IW833_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1307397.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(28321..31974)
                     /gene="rpoC"
                     /locus_tag="IW833_001269"
     CDS             complement(28321..31974)
                     /gene="rpoC"
                     /locus_tag="IW833_001269"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ1307398.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKSVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(32053..35670)
                     /gene="rpoB"
                     /locus_tag="IW833_001270"
     CDS             complement(32053..35670)
                     /gene="rpoB"
                     /locus_tag="IW833_001270"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1307399.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            36398..37366
                     /locus_tag="IW833_001271"
     CDS             36398..37366
                     /locus_tag="IW833_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1307400.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            37384..38289
                     /locus_tag="IW833_001272"
     CDS             37384..38289
                     /locus_tag="IW833_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1307401.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            38286..39092
                     /locus_tag="IW833_001273"
     CDS             38286..39092
                     /locus_tag="IW833_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1307402.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
BASE COUNT        11040 a         8210 c         6924 g        12936 t
ORIGIN      
        1 atgcggcgca cagaacgcgc cgcatatcgg actaagtttt attcaaattt gtcttcacca
       61 acacgatttg acgaagagcc ttttattttc aatcgaccat tgttgcggac gtatcggatt
      121 ttctgtatca tttttcattg tataggtcac ttgtaataca cgatcatgtt gcgcaaattc
      181 atttcttttt tcggtcaatt caaccttttt aaacgttatt tcgaccccat ctgcctgata
      241 agtttcaccc atttttaaat ctttagttac aatttttact ggtttttctt ctggaaaata
      301 tctggcttta attgttagat agcttccaaa aagaatcaat cctagtagca ataaaagaat
      361 tcctttaaag agccactgaa acggaaaata cactttaaac atctttcccc tcctccgttt
      421 tgcatttgtt ttttagataa tacgataact tttgatcaat ttcatccaac tttcccacca
      481 caattgattc tatttcagaa attttttgtt caattccttt gctttcagga ttacttaatt
      541 ttacatttga ttggttagct aacactggta aatttgatac catcacacga aacatattgg
      601 cttttgttcc gaaaatccct ttgttctgtt cgtaaaaact gtcaaaaatt gttttagttt
      661 cttttgaaca atacacttta atatacggat cagacatgtg tttttactcc ttatcttttt
      721 ttatttaggg agtctgtagg gagcttgctc cctcagaggt tttccccatc taacttgcca
      781 gaaagtagcc ctgcgtaagc aagggcggat tgactggcaa gtagatgggt agctggcgta
      841 tgccctcaaa accgcctctt tccaattccc tccgtatcga tgggaaactg gaaagtctac
      901 tggctaattc ctttagaaat ccatctcaaa atctcgttgt ctcctttgct gattttcaat
      961 catttgttgc gtgaactcgt aatctttttt agcacgatac attttcacat cgtcattcat
     1021 tatgcgactg atttgatagt gaatctgttt agcagaaggt gtatgagaac aatttttaaa
     1081 gccctcagct ggtagaaata attctttctg atctgtcttt ttaactaaaa ttgttttact
     1141 tttagtacta cgtaaagttc taggattcac atcagtatga agtttaatcg tgtattcttg
     1201 atcatcttct aaagatattg tgtttgcata atgcctgtct acttctcgca agaaatcatc
     1261 atatttaatg atttttttag caccctcatg atcactaaac tgcttttgtg caatggaatc
     1321 taattccaca aacggtacat ttaactcccc agttttataa tcaactccac tagctaacgc
     1381 tataatttgt tccgtatcta gatcaacaaa attattcata tgtgtttctt ctaaattctt
     1441 ttcaagtcgt tcaaaaacag attcaaatga atattttttc cctaactttc ttgcactaaa
     1501 agtacgttcg acatactgac ttttttcttt tctagattgt ttttctcttc ctaaagtaat
     1561 tagctcatct ctacgctcat agaaatttac atcttgtttg taaagaacat agccaagttt
     1621 gttatttgtt tttgaaaatt gataaacttc gatgttgtat tcttttgcta aatgatccga
     1681 aaaacgcaca aatgaattta cttcacaatt ctccaacgta gcttcaatcg cattttgtac
     1741 aaactcttga taattaattt ttccattttt cagtttactt ggatcgtttg aatagagatt
     1801 cgttctcttt tctaaatttt catagtactg attgtctaca ggttcgagtc cattttcaac
     1861 aagcacctca tcgttaattt ttcgcattcg ttctaaatct ttagggtcaa tacgcaacct
     1921 cttacaggtc tccatatcaa tattattaat aacaagatga ctatgaaaat gatctttatc
     1981 attgtgactt gcaacaataa tttgcgaatt tggaaaagct ttttctgcaa attcgacagc
     2041 tatttcgtgc attttctcaa tacttaactc tttagaaata ttcttatcaa acgaatggat
     2101 aacatgaaat gcttgtgcta tatcttcaga tttattcatc ctggttctta ctaaattcat
     2161 ttcttgcaat gcttcctcac tattatcact acaatttatt cctgagactg caacgcattt
     2221 ttcttttttt gaaggattag ctgcatattg taatagcgag gataaatttt tcttgcgtcc
     2281 tttcgatgtt atagtagctc ccaaagttgt ttcacctcat cttttaaact tgcaataatt
     2341 tgaatcgagt gaattaaatt ccggtcaact ttttgggaat actcttcttt cgtatcgggt
     2401 ctagtttgct tttctaacca atattgaaaa taatcttttt ctacttctga cagtttgaga
     2461 aaagaagatg tactccgatt ggactccaaa aaaatgtcat ctggataact aatcatcatt
     2521 ttttttgcta tgctattcgc aagttcatct ttaaaaatgt tgtaacggct aacgtcaaaa
     2581 ctaaaatgca ggctcttttc tttcttattt tcagattgtt ctctaaaata tttagcaatg
     2641 atatttaatg cttttaccgc ttgattcaaa ttgttaccaa tttgatttaa ctgtttgatt
     2701 agagctaccc tatcatcttc agccaataac ggcggttcat atttgagtcc ctttaaccgc
     2761 tcacgacaaa catctgctct agaaattcct ctttgttttg caatcaatgt cagttcaaga
     2821 taagtctttt tatcgacacg aaaatttaag gcctcactat gtttgcgatt tttttcgtac
     2881 attttttgtt tatcatccat tgattatccc ctcacatttt ttctagagag caacatttga
     2941 aattgctctc tatttcattt ttcatcatta attcttcttg aatcatcagt tcaatataat
     3001 ttgtgaccgt gattttttca tttgtcgctt tctttactga gtcgcataat tcggcaagtt
     3061 ctaagaaatc ctgcgtacta atctgtagaa ttaattgcat gacaatcacc tctttatttt
     3121 ttaaatagac tatcaaacag cggatttggt ttatcagaaa tatcctgaaa cagtggatta
     3181 ttttcataag taaatcgtgg acgtttcttc tccttcactt cttgttctac aatcactttt
     3241 tcgttaggcc tttcttttcg ttcctcgata atatttattt tttgcgtctt atctccctga
     3301 cttgcttcag ctcttgtttc acgtacttct accgttcttg gttgatttga cgcactactt
     3361 tgtacatttt ctcgtacatc tactgttcga tttccatttt gactacttgt agtattctgc
     3421 aagttttctc gtacatctac cgttctcggt tggcttgtcc cactactttg tacattttct
     3481 cgtacatata ctgttcgatt tccattttga ctacttgtag tattttgtaa gttttctcga
     3541 acatctactg agcgcctatc ttgactactt gtagaatttt gtagattttc tttgactgat
     3601 ttttctaatc cggtatttga atttttagct tcttggattc tttctttagt caacgcttct
     3661 ttttcgtcca tattagcgcc tgtacgccct tctacacgtt tccttaacat ttcctcacga
     3721 ttcattgctt caggcgtgta ttttgcttcc tgtcgcttct gattaattaa tacttcagca
     3781 gacttagctt gaccgtgcat agcattttta cgttcttcaa tatcttgttt aactttttgc
     3841 tgaattgcac ttgcaggatt aatatttgat tcagcattat ttttcattgc ttcacgcata
     3901 cgttcagaac tagacataat tctatcagcg ttttcaactt tagcgccatc ttttctatcc
     3961 gaaagacgtg ttggcataga atcagcatat tttccagttt catcttcttt gtttttagct
     4021 actataggtt tgctattttt attaatagcg tacacggtac catcatctaa accttctttg
     4081 aatttattct tggtatcatt agccatatca cgcatactct taaatggttt tgttgctctt
     4141 tcactggcca ttgtagctcc atccgaagct aagcctgata atcctcgttc acgagcatac
     4201 ccaagtgttt tccccgtttt attagctttt tgagaaagtg attgaccaaa gttgcgttca
     4261 ggttttcgtt cttccccttc cgcttgatcg tttccaccac tatttttccc acctttagat
     4321 cggttcatac cacctgttcc tttacgaaac agcatagctc ccatgccaaa tgctgaagca
     4381 agctggccat agccttcttt taaaccaata tccacgccaa agtaacgcat gactgttcca
     4441 ctacccttta tcagcacaat aacagcgcag acgtacgcta ctgcttttat aaaaggattt
     4501 acagaagaac cgctacctgt tccgagataa ttaacaaaca ttgcaaacat agacaatccg
     4561 aacccttgaa atccgactgt caaataacac tgtaaaatat ctgacaaaac ttgctttgat
     4621 cgttggcctg tttccaagtc agtagcaaat actaaaactc caagtacgcg tttaaacgct
     4681 agctcgagta ttgctgtaat tataatgaaa gcactaaaaa catacgctac cgctaaagca
     4741 atcaatccaa aagaaatccc ccatagattt gcttgatagc gataatatcc tgatttaatg
     4801 ccctcaacaa aggtcgacag aaaattatcc gaaaatttgg tagcaacaaa ttcattatca
     4861 gaattttcaa ctaattcata acgcagatta tcagctattg ggttctcttt ttccattttg
     4921 tcgagtacat tatctgtcaa tacttgcgta agatccgttt tccaaaaatt ttcttttgtt
     4981 agattgttcc ggtcattcgt tttatcgatc atggaatatt gatctgtttt attgatataa
     5041 gctaaatcag taaccccttg ttcaatcagc gaccacgcca cgccttcgtc cgaatttgta
     5101 atcgtagtcg tatcattata aaatgtttta gaaaactcta ttccgctgtg aatcaacgtt
     5161 ggcattaaaa gaatcaatga aattgacatt acaacattca ttccaacaga ttttagatca
     5221 accgttccct tacctgtaat cattttataa ccaataatca ctagtgaaag tatcattaag
     5281 ccaacaacaa tggtacttaa aacagcttga tagactttgt tcaatccggt actttctaca
     5341 aagctaaaca aatctaaaac ttcgggaatc atattttgaa tgccatcgac aacagcgaac
     5401 attgatttta taacagacca gcctatccac ataccgaact ggctgtaaaa atttccgttg
     5461 tattttttca aataattctg gtaaagcgga ttagttaaaa actcagtttg actctcagta
     5521 tcatcaaacc aattgaaaat actgccttct gcaatgttac tcgtaaaaag aatcacagct
     5581 attagaacaa gaaaaataac cggtaaccaa tttgtttttc tattagcaat ctttttttta
     5641 attattttca ttgttacctc cctcagccca ttttcttagg ctctcagaga gtttctgagc
     5701 tatttttctg tcaattatga tatgagttac gtaatcatca ttaacagctt ttaaatcgag
     5761 aacaatagta tttgagcaga tactctcggt taactcaaga gtatctgcgt tttgatcaag
     5821 acagtgaatc ttatctacta gatgattttc aatcatttta aattccctct tttttttgta
     5881 gcttgattag ttctaacgct ttgtttttta gagaagtaaa gatataatta tttttttgtt
     5941 tgatacgatg atctgttttt gctcttcgta tcaagtcacg taaagtcatt gctagctgtt
     6001 ctctaacatg atctacaaaa taactattat tacaaatttc atagataatt ttcagttgaa
     6061 ctttttgttc tttttcagca gatttaattg cataattgaa ttttttgtac cagttgtcga
     6121 attgacgata atcactattg ctagccagtt ttaagcaatt cacaatactt tctccccaca
     6181 tttgtttaag aagattttct tcaataaatg tttcgatcaa ttcttcatct aatttattat
     6241 ttttagttga aagtgattca aaaagatcat cattatgtga ttctttaata tctttactat
     6301 ctttataaga cttatataat tctagttcat tttttgaact atcagcaaaa ttgtttttgt
     6361 ctcttgtttt cattgatgtg tcgcatagtt caaattttga actatcaaca gaatattgat
     6421 ttgttccata gttcaaattt tgaactatca agtttttttg actattttct tgatgggtta
     6481 tcttattaat tttataaaca tcatctttgc acagtacagg ctttaacaaa taataaaaat
     6541 ttgggaacat ttgcatttta tttttattga agccattttt tcttttcaca agaagtttaa
     6601 ctttttgaag ttcatctagt gctgataaga ttgtattttt tgacattccc gaaatttcta
     6661 cgagattttt gatttcaaat atcaaaaaaa ttttttgatt ctcatcatac aagttatttt
     6721 tcaatgaaag tctatttcta tcttttagta acatataaac taacttagaa cttagtttca
     6781 ttttcttata tttttcattt acgtttaaaa aaataggata ttgatagtat aattctgcat
     6841 atatatcaga attagaatag ccttgctttt caactaagct ttcaacttct ttttcattca
     6901 ttgtcacacc tgcttctgat tttatttgct ataatagatt caaataaagt tttctcagaa
     6961 ctttattatt gagtgttccc ccgatctgca aactgacggg aacactcttt ttaatttttt
     7021 ctacttcatc attattcaaa aaaacacctc gttttttaaa tttgatgact caatatccat
     7081 tttttgcatt cttcttttga aaacaactcc attttttcta ataaaataac tggcaatccc
     7141 attcttttaa atttcaaaaa agttgtatat gatacattca aatatttagc catctctttt
     7201 ccttttagaa atggtctttc taacccggta tcaattcttg cttgatcaat agcctctttt
     7261 gtcatttcaa aaacataatt tttcaattga ttcgtttgtt gtttatctaa cattactgta
     7321 attcctgcat ccattatttt tctcctctca ttttctattt cttcaatcag ccaactcgcc
     7381 aagttgtatt tatttagatt tcaaatcatt aagtaaccaa tttaccaact tttgataagt
     7441 agatgctcta atactttctt tgctcccatt ttcgattttg acatatgttt tccgatttat
     7501 acctatcaat tttgctgcat cgtttttagt aagtataaga tcggctctat gtcttcttaa
     7561 gcttctaatg aattgatcat taagagttat cattcagcgc ctccttttaa attattcact
     7621 ttgtggtttt tatcttaata agcacatttt aaaccacgaa acgagtaatg tcaattaaaa
     7681 cattactcaa tttgtggttt ttatgctata atagtttgga atttactata gggggtgtta
     7741 ctttgaatag aataaaattt tatattgatt ccagtaaaaa gaccattaaa caaattagta
     7801 cagaaactgg tattaactat acaactctta cgaactatta ccgtggtgag cgaaatccta
     7861 gaaatactga aacatggaat atccttgctg attatttcca tgttcctgta agttatttga
     7921 tgggattaga cgaaacaaat tccatagaac aacctaatct tgatagaata aaaactgctc
     7981 gtttagaaaa aaaggacaaa atagtcaaga atattgccag tcttgactca gattatttta
     8041 ctgaattatc taatctatac ttttccatga cttctttact tgaagtcaaa cttcaagaag
     8101 atgactccga ctttcttttt ttaaaaagta ttctagagga ctcctttgaa tacacgattc
     8161 tatttgccaa acaaaagaag tttattaata aagctttaaa cttaaatatc gatgaaagaa
     8221 aagagttaga atctgagtta tttggcaact taacccaagt acaggttaac aatctaaaaa
     8281 gagagataac agctcgatta atatcaaaac ttcaataaaa ctaattattc aatcagccaa
     8341 ctcgccaagt tatcagattg gagagattag ttatgtcaat acaaaagtac acaacaaaaa
     8401 gcggtgcacg atatagtgtc cgcttgtatg ttcgtaaaga tgaaaatgga aagcaagaat
     8461 actatgtaaa aaaagggttt aaaactgaaa aagaagccaa gttacacgaa gcaagaaaaa
     8521 aggtcgagat tgaagatacc ggctatcata agccttctaa tgacttgttt ataacagtgt
     8581 atgaagaatg gttgccttct tataggaatc gtgtacaaga aacgacatat caacgtacaa
     8641 aagacctttt ccgcctgcat atactaccaa aattcgggaa taagtccatt tctaagatta
     8701 ctcctgttca ctgtcagaat gcagtcaatg aatgggcttt aaaattcaag aatatcaaac
     8761 agcttaagtc ttacagctct caaatatttg agtatgccat ttttgcagag ctattacacc
     8821 gaaatcctat ggcaaataca aatctaccaa agcgggaaaa gaaagaaaat gaaaattact
     8881 tttcattaga agaactaaaa gctttttggg atattctcaa taaagaagag cctttaaaac
     8941 atattttaat cttccagctt ttgattacta ctggtatacg taaaggagag ctagctgctc
     9001 ttcattggtc tgacatttat tttgaagaac agctaatgta tgttagaaag agctatgcaa
     9061 ctattcgaaa tgaagagaag aatgcaagaa gaaaaactgt caggattcaa aaaaatacaa
     9121 aaaatatatc ttccgaacga atattaccaa ttgatgcaca aactattgaa atgttaaaaa
     9181 tctggaaaaa agaacaagct cgagagcttt tgcagtttgg cataaataca aatacaaaaa
     9241 atcaacttat ctttacctat gtaaatgcag acggtgacat taatcaacct ttgcatgcgg
     9301 actattcaaa taatatattg aaacgtttag agaagaaata taaatttaag cacgttacta
     9361 ttcatggatt ccggcacact cacgctactc ttctactaga aggtggagca agtatcaaag
     9421 aaacacaaga tcgattgggt cataaaaatg ctgaaactac ccttaacact tattctcatg
     9481 ttactgaaaa agctcaacga aatgcgttag aaaaattcat tgcttacact ggtttataat
     9541 tttgtcatta cctttctcat taccagaacg aaaaaaagac accttctcgc aatgagaaag
     9601 tgtctgaaat cgttggtata acaacgatta acgttttgaa aactgtgaag ctttacgtgc
     9661 tttcttaaga cctggttttt tacgttcgac catacgtgcg tcacgagtaa gtaagccagc
     9721 acgtttaaga gctaaacgga agtcaggatc aacttctagc aatgcacgag cgataccatg
     9781 acggatagct cctgattgtc cagcgtatcc tccgccgttt acgttaacaa atacgtcata
     9841 tgcgcctttt gtttcagtaa cagcgaaagg ttgattgatt acttcacgca agtcagcatg
     9901 tggaatgtat tcttcaacat cttttttatt tacagtaatt ttgccagttc ctggtactag
     9961 acgtacgcga gcaactgcgt ttttacgacg gcctgtgccg atatattgaa cttgtgccaa
    10021 tgaaattccc tcctattaga ttaagtttgt gatgtctaaa acttctggtt gttgtgctgc
    10081 atgtggatgt tctgctccac cgtatacgtt caattttgta aattgtttgc ggcctaaagt
    10141 gtttttagga agcatacctt ttacagaagt ttcgatcaaa cgacgagaat ttttagcacg
    10201 caattcgcca gcagtgattg atttcaatcc tcctgggtag ttgctgtgac ggtaataaat
    10261 tttgtcagtc gcttttttac ctgttaattt cactttgtct gcattgatga cgatgacgaa
    10321 atcgccagta tccaaatgag gtgtgaaagt tggtttattt ttaccacgta gtacagatgc
    10381 aacaactgtt gaaagacgtc ccaatggaac atcagttgcg tctactacat accatttacg
    10441 ttctacttcg cctggtttgg ccatatatgt tgtacgcacg ttattttcct ccaatttaag
    10501 ttctgatgtt tgacatacac ttcgagtttc cggggctctt tgtggggcaa acaataccat
    10561 ttaacatatt acccgctaat ctagtcaatg tcaacgtttt tctgcttgat tgaaaaattt
    10621 atatgaaact ttacttcttt tctttctttt atataaaagg gtcgtttcct gtcaaaatga
    10681 aaaacaaaaa gaggctgtga caaaagtctt gtcacaaccc cttattttga ataaacggtg
    10741 ttctcctgcc aactcctcgg tcacaagtca caatatccat aaaacataag aagctgtttt
    10801 ttaaaattgt ttcttgtgac tcggagctga acggcaggct tacaacctct tggtaaacgg
    10861 tgttcaatca gctgaccctc ggtattagct caaaaaacgg caaaatatga ggaactattt
    10921 tgacgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttttcggtc
    10981 aagttcaaca acagttttta cgcgtcgatc tctggcgctt tgcccaaatg agcttggatc
    11041 atccagatac gtttttctgt ggctgttttg aattcgataa agatatcttg tgttggatcg
    11101 tcgccttctt ctccagaaac ttcgattcct ttttggtaaa gatctgctag ataacgatag
    11161 cctgcaacaa gtgtttccaa acgttctgga atagtacgat cccagcggcc aatttcatca
    11221 gggatttttg tgttatctgc aaattcacgc aatgtagaat aaggtgaacc gtctaatgtg
    11281 atcaagcgtt cggatacttc atctaactga gcgttcaaat catccatgaa ctcatccatc
    11341 attgggtgca atgtaaggaa acctggtcct ctcatgtacc aatgtgtttg gtgaacaaca
    11401 acagacattt ggcttagatc tgcaactaat tgatttaata cttctttggt ttgttggaat
    11461 ttcatctttc ttgcctccta acattttctt acacttaaaa taatagtaga ctttcaatga
    11521 aaagtacaac aaaatcattt acctgagaac gattttcagt tcgctttttt atccgtaaag
    11581 ccaatcattt ttcagaatca gctaattctt ctcgcagttg agcaaattgc tgataagtct
    11641 gtgctaaatg cttttgtgtc gaaacaaata atggatagat tttctgatat tttttatgat
    11701 cagtttcttg tactccaact tgctctttca attctatcaa ttcctgtgct tcactgatac
    11761 tttggatctt tccgatgctt ctccagccta aaatcacagc tcctaatgcg gatgcttctg
    11821 tgacagtagg gaaaacaagc gggtaaccca gtacatcagc catcatctga cgaaaaatcg
    11881 gagattgagc aaacccgcct gtagcacgga tctctgtgag ctgattttcc tggcaaactc
    11941 cttttaaaat ccgataaagg ttgagacaga tcccttctag aatcgccttc atcatttctt
    12001 tttctgtatg ttgccgctgt aaaccgataa aactgccaaa cgcatctgcg tcccaaagcg
    12061 gtgctcgctc accaagtaaa tagggatgaa aaatcagacc attggcaccc gctggcacaa
    12121 aagcgatagc cgaaagcagt gcatcatatg gattttctcc tttttcttta gcagattgaa
    12181 cgacttcctg taaaaatgta tgccctgccc aatcaaagat gccagcacca ttgctagtcg
    12241 ctcctcccac tacccaatgt ttctgatcca gaacataaca aaacgtctcc atttctggat
    12301 ggaactgagg ctgagtagta ataaaacgaa gtgctccgct cgttcctacg gtcaaagcgg
    12361 cgatcgtatc accaaaagca cctaacccta gattagaaag cggcccgtct gcccctccca
    12421 aaacaaatga tatatcattt ggcaaaccta gttctgctgc tgtttttgat ggtagacctg
    12481 ttacttgata ggtggaagga aagacttgcg gcaattgctc tattcgaatg ttcaagtagt
    12541 caagcacttc ttctgcccat tggaattcat ggatatcaaa atatccagtt cctgaagctg
    12601 ttccgtagtc gctgatcagt tcgccaaaca atctataaaa gatatagtct ttgataccga
    12661 caaaataagc agtttttttg aataattcag gttggctgtt ttgcagccac ttgattttgt
    12721 tcaatggtgt cattggatga atcggcgtcc cagtctgccg ataaaattct ttgccaagca
    12781 cggtatattt cagtttttct gcttcgtccg ctgctcggtt atctgcccaa gtgatcattc
    12841 gagataacgg tgttttctct tcatccaaca atatcaggcc atgcatcgca gaggagaagg
    12901 agacaaattg aatgattgtg tctgaaggca actgtttaat tacttctttt ataacggtgc
    12961 agacaccttg aaagatcaat tcctgatctt gttctgccat cccttgtgtt tcttggatca
    13021 aagggtagcg aaaataagcg gatgctgctt cttgggcttc atctgtgaaa gcaactgctt
    13081 tggtttgtgt ggttccgata tctaatccga ttgaaacgtt cattttttca cctcaaattt
    13141 tatgtatgta caaaaattta gtttaagctt gcgtatcttg gttttgaaaa gggggagatg
    13201 gcgtgttact ttattttatc attggttgct gtatcggatc atttttatgt ttaaccgccc
    13261 agcggattcc gttaggtcac tctattattt atcctagatc tcattgtgtc aaatgctgtc
    13321 gttcgctttc ttggtatgaa ttgattccga ttctctcgat catcgtccaa cgatttcgct
    13381 gccgctattg caggtgtcgg ttgcccttct actatttgtt ggcagaagcc ttatgtggtg
    13441 gtctttttgc ctggttcttt actttttctt ccgcaagaaa tttttcgacg ttcatatgga
    13501 tgctttcggc tttgctattt tcgttgatgg atttatttta cttcatggtc cattctcaca
    13561 cgctattctg ttcttgggca attttatgga ttttttggtt gcaggccggc gtcttccaat
    13621 ggcaaagtgt gctgttaacg tttatcgtag gcggtgtgtg ccttcgctat ggtcaatcct
    13681 atctcggagc tggcgatact ctgttgatgc tttcttggag tggtggcttg tctctagaag
    13741 agttgcttca gatcttgttt cttgctagtc tgtttggaat atccgttttt tctgtttact
    13801 ttatctgtca taaaaaaaag ttggaaaagc tgcctttcat tccttttttg agcagtgcgt
    13861 tgctgattgt ccttcatctt aggtattgaa aaaaggttgt gacaaacgac ttgtcacaac
    13921 ctctgatata tagtgttctc ttaccaaatc cttgtgactt ggagctaggc gacaggttca
    13981 caacctctcc actaaatggt gttcaatcag ctgaccctca gtattagctc aaaatgctgg
    14041 ttttacaacc tctgatatac ggtgttccat aagtagcttt ttcggcttct tatttcttga
    14101 cgccccaatg tcgacgaagg aaacctgcac gtccagaacc ttctcgataa agattgtaat
    14161 ggagcttgtt tttcttgtaa taatcctgat ggtaatcttc tgcaggataa aattcgcttc
    14221 ttggttcgat ttttgtcacg attggcttat cgaatcgccc gctgtcttgc aatgcttgtt
    14281 ttgaacgctc tgcaatattt cgttcttctt catcattata gtagatcact ggacgataag
    14341 aatcaccgcg atcagcgaac tgtcctaatg cgtcagtcgg atcggtttgc tgccaataga
    14401 tttccaccaa ctgatcataa gaaataactt ctggatcata ttcgatctct actgcttctg
    14461 tatgccctgt tgtgcctgtt gttacttgtt cataagttgg atttggtaca tgcccgccag
    14521 tataaccgga tgtcacagaa atgatccctg gctgctcttc aaatggtttg accatacacc
    14581 aaaagcatcc gcctgcgaaa actgcttttt ttgtcatata aaatccccct tttttctcat
    14641 cataccttga tttccaaaag gaggataacg ttttgcttgt gacagaaaaa acggaaacta
    14701 ttatatacgt gatacggctt ctgataaatg ccaaaacgaa cgtacttgat caaaaacggt
    14761 taaatcagga atcacatatt cagcagaagg aagatttatg ttttcaccta ttcccaatac
    14821 atgcatgtta ccagccaaag ctgcttgaca accagcttct gaatcttcaa tgaccaagca
    14881 tgcatttggc ggtacattca attgctgtgc tcctttgaga aaaacttctg gatcaggttt
    14941 tgcttttgat acttgcgtgc cgtcaataag gacatcaaaa taacttgtca aattcgtttt
    15001 ttctaaaatg aggcgtgcat ttttgcttgc tgatcccaat ccgatcttga tgtttgtttt
    15061 tttcaagtac tcaagaacag ctattgcacc aggcaaaacg gatgtttcat ccattttttg
    15121 aatcgcttga agatatagat tgtttttttg ctcagcaaag gctgatttct ctttttctga
    15181 gaatttctgt tccatttttc caaatgacag caatcgctct agtgactcgg ttctggatat
    15241 tcctttcagt tgctcgtttt ctttctcagt gaatggaatg gacagctggt tagctaaaac
    15301 cagccacgct tcgtaatgat aacgagctgt gtctacaaga acgccatcta aatcgaatag
    15361 tgctgctttg attttcatcg attacctcct tgaaaatgaa cgacttgaag taaagatgcg
    15421 atgtttttag ctgtgcgatg aagattatca gtggcttgct gtaaagcttc ttgtaaaggc
    15481 acagctgcag gaaggatcgg aaaaacggcc gaaatgccaa catctgctgc tttttcgctt
    15541 cctttaccaa cacttccgca aatagctatc acaggaacgc cctctggtac tcttctagca
    15601 atcccaactg gcgcttttcc ttttagactt tgtccatcta gacgtccttc gcctacgatt
    15661 acgaggtctg cttgctgtac ccgtttatcg aactccagta aatccaatac gtagtcgatg
    15721 ccagagacta ttttccctga agcaaatgct aacagtcctg cacccattcc accaccagca
    15781 ctactgcccg atgcctcgat tacttgaggg gcaaatctca tataaaaatc atgcatggct
    15841 tggtcagcca ctgctaattc attttctttc aagccttttt gtggtccgaa aacaaaagaa
    15901 gcgccttctt ttccgcataa aagattagta acatcagtaa tgacagtgat tttggcttta
    15961 atcagctcaa taggcacgtt cgttttatca actgtagcaa ttttacctag attttctccc
    16021 agtgcttcta ctttttgatt atcttgatcg taaaaagcat aacctagacc ttctgccatg
    16081 ccgatgccgc catcgttggt actacttccg ccaacgccga tgatgatgtg tcgaacacct
    16141 tgttgcaaca attgtcgtat cagttctcct actcctcgcg tggataacct aagtggctgt
    16201 tgttggttta gtgggacatc tttcaatccg acgatttctg ccatttcaaa aatcgcagtt
    16261 tgaccgtctg aagcgtactg gatttctttg tttttgccaa atgcattagt aacgactgct
    16321 gtttgccgct tcaaccttaa actgtctgct aatgatgaaa ccgttccttc tcctccatcg
    16381 cctataggta gcagatcata ttcagcctga acaaaaactt cttgaaaacc tgattgaatg
    16441 gcttgtgctg cctctaaggc tgtcagactt tctttgaaag aatctggcgc aatcagaata
    16501 cgcattattt tcccctccgt tccaagatca gttctttttc cagctggtat tggtctccat
    16561 tgacaaaaat aggtaatgtt tctttcgtct ctttttttat tactatttgt ttttttgtca
    16621 aaacaaatag caacctgtga tttttccaaa taaaaggaaa agaaattttt tcccatgctg
    16681 aaggaagtct tggagaaata gaaagagttc cattcgttac tgtaatacct gaaaaaccaa
    16741 atataactgc tagccatagt gctcctaatg aagcagcatg aattccgtca tctgatgaat
    16801 gcggatctgg tcctaaatca atcaggcacg cttcttggaa taaatgatac gcccattcca
    16861 tttcttggat acggcaagcc tctatggcgt gaatcgcttt actcaacgat gagtcgtgga
    16921 ttgttttgtt ttcatagtaa tgcaaattct tagaaacgat tggttgagaa aaatactcag
    16981 gtaataagta taacagcatg acaagatctg cttgcttgct gacttggagt tcatttactt
    17041 cgctacgggc ataatcaagt aaaatagact ggctgccagg acgttctttg taagtggtca
    17101 aatcaataac tggcttactg agatacgtgt catcttgcgg aatcagctgt tctttgttag
    17161 gcactggcaa atataactta tctaaaaaat tttgacattc tttcaaaaaa agcggatcgc
    17221 cagcttgata tttaacagct aaggccacat tataatgcgc cagatagttc gtatatgcat
    17281 tattgtcgat atattctgtg tattcatctg gaccaatgac tctgtgtatc tctaaatgat
    17341 catcgacttt tacagctctg ctcagccaaa aagctgctgt ttcatgaatg agcgtttttc
    17401 cctttgtttc taaaaacgat tgatcctctg ttgcttggac atactcttga acggcataag
    17461 caaggtctgc actgatatga tgttctgcgg cagctgaagc aacttcttgt cttgttccgg
    17521 ttttgatatt caatgcagca aatttgggtg tctcttcctg accagtgtgt gcgctttccc
    17581 atgggaaaag tgcaccttga tagtgataat ctcttgcttt ttgccgtgcg ccttctaatc
    17641 gatcataacg gtacagcagc aaattctttg ctacttcagg aaactgatat aaaaagaaag
    17701 gcaggataaa aatttccgta tcccagaaaa catgcccttt ataaccttcg ccagttagtc
    17761 ccttcgcccc tacactaaca cttggatcat tgcggggagt cataatctgt aaatggtaag
    17821 cagcgaagtc catcgctaac tgatcgaaag aagaatcgcc ttggatcgtt atccgatgct
    17881 gatgccaaaa atctgtccaa taagacatcg actgctctaa aatttctgta taactaacgc
    17941 ttttctgggc ttgggcaata cttttcttaa acgggtctgt ttgctgaaga gaggtagaga
    18001 gcacgatgat cttctccagc gtaaaggacg tattttttct gatcttttct gaaaaggaag
    18061 cggtgatccg gcgatttttt gcttggaata cacctgcttt attgaattta gctgcaatcc
    18121 caatatcaat tttactttct gttgtctggt aggatgcact gatcaaatcg tcaccaaata
    18181 ctcgcagttc tttttcaacc aactgctgtg taccaaaatt ggtttgctgt ccatcgatac
    18241 ccgttttcac atctacttgg cagtcttcgg tcaacggctt gatgaccact ttcagggctg
    18301 cctgatggag attattttga tcagcaacac gacaagtaat gacttccact ttttttcctt
    18361 gagcagtttc aatgagcgca tagcgtttaa gctctcccat ttctaaatct agtgcccgct
    18421 cgtatgcaag aactttcatc gtctcaagag aaaaatgttc accgtcgatt tttatttccc
    18481 atctgatgac atctgggaga ttcactaact cggctgattc tgctcctaaa ggtcggttat
    18541 atacaccagc tgcaaacatt ccccttactt gattccgata gtcttcctcc tggcttcctc
    18601 tgataccaaa aaatccattt ccaatggtca tctgactcgc atacttattg atatactcta
    18661 aatcaaactg tttttcttct actttcataa ggtaataact tctcctgttt ctgccgaacg
    18721 atataaattt tccaccagat tctggatgac atatccttgc cagccatctg caatcatctg
    18781 agtctggtca ccgagcgccc gattcacaaa atgagccata cttagttcat gtgggtcatt
    18841 aactggcgtg gtcttttgta aggaaaccag ctttccatct acatcttcga atagttcgcc
    18901 tggatacaga ttagcacctg cctgatcgcc acaaaaagcc acattcagtt tgtcttctct
    18961 gatattcaaa gcaaacgaag tattcagttg gatgatcttg ccgttttcta attccagtgt
    19021 gccaaaaatt gcatcttcta ctgtgaattt gcttggatcc catttcccga atttccctga
    19081 attttttttc gtaccaattt tttggaagct gtacgcccga acgcttttta ctttaggaaa
    19141 tccagtaata tacatcgctg tatcaagcat gtgaatgcca taatcgatca aaggcccgcc
    19201 tccttgaagt tctttgtcga taaacgttcc ccagcctgga acaccagacc gacgaagcgc
    19261 attcgcttct acgtaataaa tatccccgag aacatcaaga tgattcttga tcattgttgc
    19321 ttcgtcagaa aaacgatgct ggaatccata agccaaaatc tgcccgtttt tatcggctgt
    19381 ctctgccatc tttcttgctt gatcagctgt catagctggt ggtttctcgc acatcactga
    19441 agcattagct tcgagggcag ccaatacatg ttcgaagtga aagcgattgg gtgtacagac
    19501 actcacgata tcgggagaaa tctcattcag catttgtgta actgatgtat acgcatgaga
    19561 aaatcctgta cgtcggcaaa agtcttctgc tcgctggctg actggatcaa ccacagcggc
    19621 caattctaag acatctgatc ttttttgata ataagcagca tgtacttttt ctgctacttg
    19681 tccagcgcct atgattgcta ctttcttcat ttatttctcc ctcctttgct tatttactta
    19741 ttgttattct ttctctattc tttctctaaa tcatttaaat gacgcgctgt ttccagacag
    19801 tttttcaagt attgcaatga ttcctgataa attttcactt cattttctcc tcgcaaacga
    19861 cattcatata ccatataacc ttcatactca ttttgaagta gcgtttgaaa atggcgagta
    19921 aaatcaattg cgccagatcc tggttggtag cgatgattat ctgctaaatg gatatgaccc
    19981 acatactctt tttgcgtagt caatgctttt gaaatatcat cttcttcaat attcatatga
    20041 taaaaatcag cgatgatctg cacatgtttc aattgattag agtcaatgta atgacgcgca
    20101 tccgctaaca aattgatcat atggtcttgg tatcggttca aaggctccaa ataaataacc
    20161 gtacctgttt cttcggctac tttatctaaa taacgaagtg attcgctgac tgctttatag
    20221 tctccttctt tgcttcgggg tgaaaccatc ggcggtaatc gataagtgaa cattccccaa
    20281 gctgctggga taacgattcc tttgcctcca acctcgctta gtgcacgcaa aatctcagca
    20341 atttgacgta caccattaag acgccgttct tcgataaagt caccgatcca cccatcgtag
    20401 ccgttacaag ctgtcactac tgggatccct gtttctttga ttgcctgttt gactgtgtcg
    20461 atattttcta ttaacaactt accgtcgatt tcgtattctt caaatccaca gtctttgata
    20521 aatctaaatt tttctaaaat gtctcttggg aaaaatgctt gatcttgcgt accgaatttc
    20581 attgttctcc tcctcggact tgaacgccca ttttgatact ttcttctggg tgctggtcta
    20641 cataacgcat gtaagcttct gcgctttctg caaatggcac aactggatca atgatttctt
    20701 cacaatccaa gtaaccgttc atcaataatt cccaacatgt gtcctcgatt cgtttacggt
    20761 tccatcttgg gtaatctggg tttggttcac tagatgctcg agcaaagacg atttttgcat
    20821 tattgaaatg tgcttcgcga ccaagattaa acccagctgg aaaaggttta gcaaaggcaa
    20881 cataagaaat gatcccgcca tagccgatcc ctcttaaaga agcttgtaat gcggcagcac
    20941 tgccactcgt ttcaataact gaatctactc cacgtttatc tgtcaattcc tttagacgaa
    21001 aaccgacatc tgctttcata ggatctagcg tgtaatcggc accgtatttt tctgctaatt
    21061 ctctacgctg cgctatggga tcgatcccaa cgatggtagt agcacctgct tttttagcta
    21121 attgaatcgc aatttgcccg atagcgccta aaccgaagat tgctacagca tcacctgctc
    21181 gaacatgtgc ttcccggata ccacctaaag cgaattgagc aggatcgtaa catactgcat
    21241 ttttccattg atccaaccga tcaagtttac gtagtttata attatcaaca ccgtttgtaa
    21301 tcagcgtttc acgaatgggt ccataagtgc agactaactc tcctttttga tactctgtca
    21361 ccaaactgcc cgtttcttcg atcgtaccga caatcatatt tcctaattga aattcgccaa
    21421 ataccacacc gcgttggctt ccttcttctc ttggcaagaa taggcgccat tcttctgaaa
    21481 attcttcatc gataaatgga cttaatcctc taaagtcgac aatttctgtt cctggttttg
    21541 gagtagcgaa aattactcgg atacgtactt cgttcttttc gatctcacgg tcttcgtaat
    21601 ctttgaatcc ggctattctt ggttctaatg caactaattt tttcatcttt tctttcctct
    21661 ttcttttctt tttatccttt tacgccgcct tcgactaaat cacctttaat aaatcgttca
    21721 gataacgcat acataatgat gactggtaaa gcggtcacca atgaagcggc catcattcgt
    21781 ccccaaatgt agtctggtgt actgaataat gtgttcaaac cgattggtaa agtaaatttt
    21841 tcggaggtcg acaggaaaat cgaagcaaat aaataatcat tccacgaaac cataaagcaa
    21901 taaacgaaga cagaaatgat tccagaaata gccaatggta taatgatccg aataatgatc
    21961 tggatccggc taagaccatc gatcattgct gcttcttcaa tatcagtcgg aatcgtgtca
    22021 aaataacttt ttagcatgaa tatcgcagta ggaagtgttt gaacgaccat cgtgataatc
    22081 agtgctgtat tggtatcata caaacctaga ccggaaatga ttttaaataa tggaacaacg
    22141 atcaaaatac cggaaaacat ataaacggtg taaaagctag cattgatttt cgagcgagct
    22201 ttgaagcgta gttttgacaa agcgtatgcc ccgctgatcc caatcactac tgcagttacg
    22261 gaggctatca aggagatcac taaactattt ttgaaataca tgatgaatgg aaaaatctca
    22321 gcattgaaaa tatcctgata atgttcaaaa gtccattctt tcggaaaaat agtttgtgtc
    22381 gttgcaattg cttcttgtga atccttgaat gatgtcatca gcatgatcaa aaatggaaac
    22441 aaattgacag ctaatagcaa caaaatcgtc gcgtaaaata agattttcct gctcttttta
    22501 tttttcattt caagtccacc ttctttcgtg agagcaagac gaccgctgca ataatgatga
    22561 acaatagaat cgatatagct gctgcttttc caagatcatt gaacgcaaag gctgtctgat
    22621 acaaataaac gcctaagata ttgactttat tcgtcagtag atagacatct gtaaacatat
    22681 agaacatcca gatagatcgt aaaacaatca cagttgctag tactggtttg attgccggca
    22741 gtgtcacgat cttgaacttc tgccaagcat ttgcaccgtc catttcagct gcttcataaa
    22801 gcgttttgtc gactgtttgc aaaatagcca aaaatgaaat aaacgcatac ggatagtatc
    22861 tccagatagc aaacaaaacg accataaaga agctagcaac tggctggtcg aaccacagcg
    22921 gtgcttcgtt gaaaagctgc aacctatcta ctaacagatc attgacgatt ccataactat
    22981 tattgaacat atatttccaa gcaaaaatca aagaaataga tggcgtaaca taacttaata
    23041 aaatcagtga acgcaccgtt ttcctaaatc tgaacttgcg gttaagcaaa atagctacgg
    23101 ctaatcctaa agctgtactg cctgctacaa ctaagaatgt gtaagcaact gttaaaccta
    23161 aagaacgata aaattcacta tcacttaaaa tttcaagata attgcttaat ccgataaaaa
    23221 cggcgtctga gttaggattc agcggcatat cgaaaaaact gatctgaata ttggaaagca
    23281 ttggaatcag cactaaaaca gctaataaaa gcatgcttgg tgctaaaagt cccatggcta
    23341 attttaagtc agaatgtttc cttggacgat tcattgaaac ctccccctga taatctgcca
    23401 atagctatct acttttttcg aacaccattg gcagaacttc ttgttatttc gtattttttt
    23461 gtgcgtcagc taaagcttga tcgacatctt cgtttccgac agttacttgg ttcactgctt
    23521 ttccgatcac acctgagctt gtgatatctc ccattttcgt gaaattcttt tccccgacta
    23581 gtccaaagac ttgtacttca tcaaaagcac tagcaatctc agatggcagc tcgccaaaag
    23641 acttgatgac ttcattttct ttgtaaccat ccagttcgac tacttgttta ttcactggtt
    23701 gagcaccacc aggagacatc aagacccact tttccatgtt tttcggttga gataagaaag
    23761 ctgtgaatgc ttcagcagcc tctttttgtt tctcgtctag accagaagaa atcgtcaatc
    23821 cagatactgt gccatagaca gcttgatctt tttgtgttgg gatagcaaaa ccgatatttt
    23881 ctgagttccc cgcttcataa acagaaggca aaatgtaagt tgaatagatt gccattggtg
    23941 ctgttccatt catgaaagca tctttgattt ctgtcacatc attagagcct ggcatcgtgt
    24001 attgggataa gttttgataa aacgacaatg cttctctcat cttttccgta tcgatcgtta
    24061 cctcgccttc atcatccaaa acatttgctc catttgacaa agcaaattga gagaacgctt
    24121 gctcactcat tgttccttca gcagtaggca tagcaattcc atattgttta ttctctttat
    24181 ctgtgaaatg ttgagcaatt tttaaaatat catcccaatt ttctggttct gaaaaaccag
    24241 cttctgataa tttttctttg tcataccaaa taccttgaac ccagccgcta attggtgcag
    24301 caatatagct ttttccatct tctgaacgaa caaggttttt ggctccgtca taataattat
    24361 cttctccagc ttcctcgata acgttttgga ctgccgtctg atcaatcagt tcatctttat
    24421 ccatcacttt agcaaaatcc tgactgactt ccattacttc tggtaattta ccggaacgag
    24481 ctaacgtaac gatttttgta ttgaacgctg attcttctac cggcacttgc ttgattttga
    24541 tgtctggatt ttctttttca aaatcagcaa ccagttcatt gatcacactt aatcgttcct
    24601 gttcgacaga agagtgcatg aattgaatcg ttgtctgttg tgattcgtta gagctttgac
    24661 tttcaccaca agcagtaaga agaaatccag tagctagcag acaggtagta acaataattt
    24721 ttttcatttt tttccacctc ttattttatt attttcagcc acatatattc atacggctgg
    24781 aatatcaact ttcgttcttg actgcaatgg ataatttgtc cagaaaacag gtctaggtaa
    24841 tctccttttg gtaaaagata acctgctgat tgattggaaa aattatggat aactaagact
    24901 ttctcttcct tcgcttcgcg aataaaactg ataaaagaat ctgctgagtc cagtaagtaa
    24961 atcaaactat tcggatggaa ggctttttct ttttttcgaa tagatagcat ctgggtcaat
    25021 tcaccatata ctgctgttct taatggatct gtctgcaact ctttctcaat atctgctaac
    25081 aaaaattttt gccgattgat ttttcgattc tctcctgttt tttctacgcc tttgtaatca
    25141 tttcgacttc ccagcacact ttgaatataa attgccggca cgcctggtaa cgttaccaaa
    25201 aatgaatgag cagctaaaaa gcgtttgctt ctccattccg tcgtatctgt tggagaactt
    25261 aacgcatcta gatacgtagc gtttatctca tatggactgg tcgttccatc tgggtttttc
    25321 ttgtagttta ctcgggcacc ctctttcttc agctgttcca ctaattcaag gatcgttttt
    25381 tccggcagaa tacctcgtac cggattcaat ccgataccgt catgggaggc taaaaaatta
    25441 aaaaaagttg tttttttcga tgcgaaatcg atggttttta accattcttt caacttactt
    25501 gtattacccg aacgaatcgc ttgaagggtc aaaggtggta gcgaaaactg atagaccatc
    25561 tgtgcttcat tctcgccatt cccaaaataa ctgatattat ctttgtgtgg cacatttgtc
    25621 tcagtgatca ataccgttcc tggagctgct tcatcagcga ttgcacggaa taattggatg
    25681 attttatgcg tttctggcag gtgtatacaa gaagttccta gttctttcca taaaaatccc
    25741 acagcgtcca agcgaataaa tgaagcgcct ttttctaagt aaaacaaaag aatatcgatc
    25801 attttcaata aaacttctgg atttttaaaa ttcaagtcta cttgatcttc gctaaaagtc
    25861 gtccatacgt gtttttcttt cccgtttttg tcaatgaacg ttgtaagtaa cgggctggtc
    25921 cttggtctag tcacgctgct tgtatctgta tccagtggca aatcaataaa aaaatctttg
    25981 tacgtatcat tcaagtttaa atattcctga aaccaagtac tttcggcaga catatgattg
    26041 cagacaaagt cgaacattaa ccgtgtttcg cttctcattt cttctatatc ttcccatgtg
    26101 cccaaacggt cgttgattgc ctgataattt acaacggaga aaccatcatc agatgtatat
    26161 gggaaaaatg gcaagaaatg tacaactcta aatgtatctg ccaaatactt ttgataaaat
    26221 ttcttaaatg tacgcaaagt tggttgctgc tcttcaaaaa attgatcccc ataagtaatc
    26281 aagaaaatat cttcttcgtc ccaatgcagt ttgcctgcct ggcctttctt ctgctgataa
    26341 acactgactt tttgttcaat catagaggcg gttttctcag acaaatctga gtcgcgataa
    26401 atcgcttgta aaagttttgt atacatcgga ttcccctttc ttttttggga gcgttcccaa
    26461 cgatttgatc atattatgga aaacgcttac tgtcaatagc atttttttaa ataattttga
    26521 tatacacaaa aatacaaata agaaagctta tgtatcaata attaatagct tcttaaaaaa
    26581 atatggtctc tcatgatcat gcaagctatt cctaaatttt caaaatcata gacaaaaaag
    26641 aatgtctatt gtactatgga attattctaa aacgctccca aaaaggaggt gcatataaaa
    26701 ttgaaaccta ctatttatga cattgcgaaa aaagctaatg tctcaaaatc aactgtctcc
    26761 agagtattaa ataaccagcc aaatatctct gataaagcaa aagcaagtgt acttcaagca
    26821 atcaaagaat tgaattatga accgagcaaa attgcaagag gactgtcagg aaatggtttt
    26881 gacgcgattc tagcattatc tcatcgtact tctcatacga cctatggcaa tccttttttt
    26941 tcagatgtta ttcaatccat ttctgccgtt tctgaacaaa ataacttcga cttgattcta
    27001 caaacggcaa aaacaccaga agaagagcta gaaaaatgcg cgaaaaagat ccaagaaaaa
    27061 atgatccgag gaattattat tttaagctct acgatggatg aaacacttct ttttgaattg
    27121 gatcaataca atattcctat tgtggtaatc ggaaaagtcg gaaacgacta cacgaacatc
    27181 tactcagtgg acacggataa tttccaggat agttacgatt tggtggaaac actgatcaag
    27241 aatggacacc gtcatatcgg ctgtctccat gctccgacag aagtttatgt atccaatgat
    27301 cgtctcaatg gttaccgtca atgcttattt gatcaccacc tagatatccg taatgaatgg
    27361 attatagacg gcggctttcg cttagaagat agcatggcag caatgaatga tttaatgaag
    27421 ctacgagaaa gaccaactgc gattttttct accgatgcat taaaatcatt gagtatttac
    27481 aaagtggcag aacaatctgg aaagcagata cctgatgatc tttcgatcgc aggattcagt
    27541 gatcccttat tgtcctccct attttcgcca cagttgactc ggatagacat tccgacaaat
    27601 cagttaggaa tcaaagccac agaactttta ttccaactaa tccaccacca ataccctgaa
    27661 gaaaaaagaa tcattctttc aaccgagatt actctcacag attcgatcca acaacaagaa
    27721 atgtagaaca gaattataaa agaacagaaa cactgaggat aagtcagtta ctctgttctt
    27781 ttttcaatca ctcatccctc gttaccccct cccggtattt tagcgaattt ttcttatttt
    27841 ccatctgttt tagatagctg tggataacta taatgtttgt gttaaactat tttttccaca
    27901 gggaagaaaa aactattttt aaccacgttg tgtataagtt tttatagttc gtttttttaa
    27961 acacatttac ccttatttca atccatgtta tttggggata cttatctcta aaacataaat
    28021 agaataacag acatgtctac tggttttgtt ttattcgagg aattattcaa taatagctag
    28081 caaaatatta aaaataaccg ctctatcttc ccatcaattt attttgcatt caaaaaatga
    28141 tctatcttta cttttgttga ttgacttctc ttggaagatg atcgttccga taccttaatt
    28201 ggatacgaac cgatgttgtt aagatccttt cccacttttc ttctagttag acaaattatt
    28261 ttagacaaac caaaaaagag gttgtgacaa taatttgtca caacctcttt tttaaaatat
    28321 ctattttgtt tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat
    28381 actgtagaca ttttcgcttg ctacaccgac ttcttttggt tccatgtcac gataacgttg
    28441 cataccagta ccagctggga tgatcttacc tataataaca ttttctttca aaccaagaag
    28501 aggatctttc ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt cttggaatga
    28561 tgcagcagat aagaaactat ttgtttctaa tgatgctttt gtgataccta gtaagacagg
    28621 tcgagaagtt gcaggaacgc ctcctgagac tagcgtattg tagttttgat ctttaaagtc
    28681 tgcgatgtct aataatgtac ctggtaagat atctgtatca cctggatcca ttacacgaac
    28741 tttacgcagc atttgacgaa ccattacttc gatatgcttg tcgccgattt ctaccccttg
    28801 catacggtaa acacgttgta cttcacgaag caagtagttt tcaacagata atacatcgcg
    28861 aacttgcaac aattgttttg gatcgattga tccttctgtc aatggtgcac cacggtggat
    28921 catatcgcct tctgctactt tcatacgtgc tgtataagga acagtatacg tacgtgtatc
    28981 tgtttttcct ttgattgtta cttctttggt acgtgttgca ggatcttctg agatatcgat
    29041 gacatcccct gttacttctg tgatcactgc ttgccctttc ggattacgtg cttcaaagat
    29101 ttcttggaca cgaggaagac cttgagtgat atcgtctccg gcaaccccac ctgtatggaa
    29161 tgtacgcatc gtcaactgag taccaggttc accgattgat tgagcggcga tcgtaccaac
    29221 tgcttctccg acttcaacgt cagagccagt tgcaaggttg cgtccgtaac aatgtttgca
    29281 gacaccatgt tttgtattac atgtgaagac agaacggatt gtcactgttt cgacacctgc
    29341 atcaacgatt tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc
    29401 atttgtttca ggatgacgta cttctttacg ggtgtaacgt cccactaaac gatcttctaa
    29461 tgattcgatg atttcatttc cctcgcggat ggcttggatt tcaagacctc ggtcagtacc
    29521 gcaatcttct tcacggatga ttacatcttg ggccacgtca accagacgac gagtcaagta
    29581 acctgaatcg gcagtcttca aggctgtatc ggtcatccct ttacgagccc cgtgagttga
    29641 gataaacatt tccaagacgg atagtccttc acggaagttc gaaatgattg gcaattccat
    29701 gattcgacca ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg taaagttgga
    29761 aatgttacca cgagctccag aatcagacat catgaagatt ggatttttcg cttccaaacc
    29821 ttccatcagt ttttgttgga tgctgtcttt tgcagcattc caaacagcaa tgacacgttc
    29881 ataacgttcg tcatctgtga tcagaccacg acggaattgt tttgtaatag tttcaacttg
    29941 tttatgggca ttttcaatga tttcttgttt ttcattcagt acactgatgt cagcgatccc
    30001 tactgtgata ccggcatgtg tcgaatgttt gtaccctaag tctttcatac ggtcaagcat
    30061 cttagaagtt tctgttactt tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt
    30121 tttcttcttg aacggtaata ctaattcttg ctctttgata tgtgcaggaa tatcagtacc
    30181 agcttcaaca aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt
    30241 cagataaggg aattcaggag gcatgatctc gttgaagatg atcttaccaa ctgttgtgat
    30301 catcgtacgt tctttttgcc attcagtaaa tggtttttca cctaatgagt tagggttcac
    30361 accgatacgt gaatgtaaat gaacatatcc gttgcgccat gcgataacgg cttcgttttg
    30421 atctctgaag accattcctt cgccttcgcg tccttcttct tccattgtca agtagtagtt
    30481 acctagaacc atatcttgag aaggagtaac aactgattta ccatctttag ggttcaagat
    30541 gttttgagca gctaacatca acatacgtgc ttctgcttgt gcttcttcat tcaatggaac
    30601 gtggacagcc atttggtctc catcgaaatc ggcattataa gcttcacata caagtggatg
    30661 caaacggatt gcgcgtcctt gtactaaaac tggttcgaat gcttggatac caagtcggtg
    30721 aagcgtaggt gcccggttca gcaatactgg gtgttccttg attacgtctt ctaaaacatc
    30781 ccatacttcg tcttcttgac gttcgatttt acgtttcgca tttttgatgt ttgaagctag
    30841 ttcgcgttga acaagctcac gcatcacgaa tggtttgaat aattcaatgg ccatttcttt
    30901 tggaagtcca cattggtaca tcttcaagaa aggtccaaca acgataacgg aacgacctga
    30961 gtagtcgaca cgtttaccta gcaagttttg acggaaacga ccttgtttcc ctttcaacat
    31021 atgagaaaga gatttcaatg ggcggttacc tggtccagta actggacgtc cgcgacgacc
    31081 gttatcgatc aatgcatcta ctgcttcttg cagcatacgt ttttcatttt gaacgatgat
    31141 tccaggtgca ttcaagtcta acaaacgttt caaacggtta ttacggttga ttacacgacg
    31201 gtaaagatca ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa ccattgggcg
    31261 taaatctggt ggaataacag ggataacgtc cataaccatc cagcttggtt gatttcctga
    31321 agcgcggaat gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt tttgtccttg
    31381 tgcagatttc aattcttctt tcaattcggc tgcttctgca tctagatcaa cattatcaag
    31441 taattgttta atcgcttctg cacccatagc agcttggaat tcttgtccat attgttcacg
    31501 tttttcgcga tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg
    31561 ttcgatgact acatatgaag caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc
    31621 taatacaaga cccatacgtg aaggaatccc tttaaagtac cagatatgtg aaacaggagc
    31681 tgctaattca atatgtccca tgcgttcacg gcgaactttc gaacgagtaa cttccactcc
    31741 gcatcgatca caaacgattc ctttataacg gatacgtttg tattttccgc aggcacattc
    31801 ccagtctttt gtaggaccaa aaatccgctc acagaacaac ccttcgcgtt caggttttaa
    31861 ggtacgatag ttaatcgtct caggtttttt tacttcaccg taagaccagc ttctgatttt
    31921 ttcgggagaa gccaaaccta tttgcatact ttcgaattta tttacatcga tcaaaagggg
    31981 ttccctccat ttcatttaat ggcaaaccgc aagacggctg atcttcgacc gccttgcagc
    32041 tcatagaccg gattagtcta aattatcttc tgcggtttca aagttttgga tgacatcgtc
    32101 tttttcatct tccacttgtt cagcagcttt cttttctaat tctttggcag tttgctgttc
    32161 ggcaaatttt gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg
    32221 aagttcgatc tctgtttctt cgatatccaa gacacgcatg tccagaccta gtgattgcaa
    32281 ttctttcacc aatacgcgga atgattccgg tacacctggt tttggaattg gttcaccctt
    32341 gacgattgct tcgtatgttt tcacacgtcc gactacgtca tctgatttgt atgtcaagat
    32401 ttcttgtaaa gtataagccg caccgtaagc ttccagtgcc catacttcca tttcaccaaa
    32461 acgttgtcca ccaaattgag ctttacctcc caatggttgt tgtgtaacca atgagtaagg
    32521 tccgattgaa cgtgcatgca atttgtcatc gaccatgtga gcaagtttga tcatgtacat
    32581 cacaccgaca gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt acagaacagt
    32641 tttcgcatcg ctggccatac cggcttcacg aactgtttcc catacgtctt catcacttgc
    32701 accatcaaat actggcgttg ctacgtggat acctaactga cgtgcagcca tacccaagtg
    32761 taattccaat acttgcccga tgttcatacg tgaaggcacc cctaaaggat tcagcatgat
    32821 atcgattggt gttccatcag gtaggaatgg catatcttct tccggcataa tacgggatac
    32881 aaccccttta tttccgtgac gaccggccat tttatcccct tcatggattt tacgtttttg
    32941 aacgatataa acacgaacta acatgtttac gcctggtgac aattcatctc cagcttcacg
    33001 agtaaagatt ttcacatcgt gaacgatacc gccgccgcca tgtggtacac gtaatgatgt
    33061 atcgcggact tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga
    33121 taattctgtt accccttttg gcgtgacttt ccctacaagc aaatcgccat cttggacttc
    33181 tgcaccaata cggatgatac ccatttcgtc taaatctttt aatgcgtctt ccccgacatt
    33241 agggatttcg cgtgtgattt cttcaggtcc taattttgta tcgcgggctt ctgattcata
    33301 ttcttcaata tggatagaag tgtatacgtc atcttttacc aaacgacggc tcatgatgat
    33361 cgcatcttca tagttgtacc cttcccaagt catgaaacca actaaaacgt tttggcctag
    33421 agccatttcg ccttgttcca tagatggtcc gtctgccaga gtatcccctt tttcgacttt
    33481 ctcacccaaa tgaacgattg gacgttggtt gtagcttgtt ccggagtttg aacgacggaa
    33541 ttttgttaca tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc
    33601 atcaacgtat tcaacgacac cgtcatgttt acataacaaa gcagctcctg agtcatgcgc
    33661 tgatttgtat tccataccag taccaaccca tggtgcttga ggattgatca acggcactgc
    33721 ttgacgctgc atgttggcac ccatcaaggc acggttggag tcatcgtttt ccaagaaagg
    33781 aatacatgct gtcgcaacgg caactacttg tttaggtgat acgtccatgt agtctacttt
    33841 atcaatagat acttctaagt tttcactttg agcacgagcc ataacaacgt cttgagcaaa
    33901 tgttccatct tcattcaatg ggctgtttgc ttgggctacg atgtaatggt cttcgatatc
    33961 agctgtcaag tagtcgattt ggtcagtaac acgacctgtt tcacggtcta cacgacgata
    34021 aggtgtttcg ataaatccga atttatttac tttcgcataa ctggacaaac tattgatcaa
    34081 cccgatgttt gggccttcag gagtttcgat cggacacata cggccatagt gggagtagtg
    34141 aacgtcacga acttcataac cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga
    34201 aagacgacgt ttgtgtgtca attcgccaag agggtttgtt tgatccatga actgtgacaa
    34261 ctgagaagaa ccaaagaatt ctttgatact tgccacaact ggacggatat tgatcaattg
    34321 ttgaggtgtc aatgtttcag tatcttggat cgacattctt tcacgaacca cacgttccat
    34381 acgtgctagc ccgatacgga attggttttg taaaagttca ccgactgaac ggatacgacg
    34441 atttcctaag tggtcgatat catcgacatt gccgatacct tccatcaagt tcaagaaata
    34501 gctcattgaa gcaatgatgt cggctggacg aaccgttttt acagctgctt ctggatagcc
    34561 gtttccgatc acgtttactt cacgttcagg gtctcttggt gaaaatactt tgatgacttg
    34621 gacagtcatt ggatctgtga ccacaccgtc ttctgatgga tagtaagtca ctgaatttag
    34681 gccattatca atgaaaggag ctagtgtttc catgacttgg tgagtcagaa ctgttccttt
    34741 ttcaacgatg atctcgcctg tttctggatc aactaatgtt tcagctaatg tcagattcaa
    34801 tagacgtgtt ttcaaatcta attttttgtt tactttgtaa cgaccaacgt ttgctaagtc
    34861 gtaacgtttt ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt
    34921 tggttcgcct ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc
    34981 gcttgcgttt ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa
    35041 gatttcaaag atcgtatcat cggaaccaaa tcctaacgca cgaactaata cggttaaagg
    35101 aatcttacgt gtacggtcga tacgtacata agagatgtct tttgcatcag tttccatttc
    35161 tagccaagct ccacggttag ggataacagt agaaccgaat ccttcttttc cgtttttgtc
    35221 gacttttcca tggaaataga cacctggtga acggactaac tgagaaacga ttactcgctc
    35281 tgccccgttg atgatgaatg ttccttgttc tgtcattaat gggaaatcac cgaagaatac
    35341 ttcttgtgct ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg
    35401 tgcagagtag tttgcatcat gcgcacgagc ttctgctact gtatactttg gttctttcaa
    35461 ttcataatct acaaattcca aagatagatt gccattgaag tcatcgattg gcaaaatgtc
    35521 ttcaaacatt tctctaagtc cctcatctaa gaaccattgg taagagtctg tttgaatttc
    35581 gatcaaattt ggtaattcca acacttcact gattcttgcg aaacttctac gttcgcgatg
    35641 ttttccgtat tttactacgt gtccagccaa gctcttcacc cctctaaaat gtcattcaaa
    35701 aagactaccc gatcaattgt tacaataata tttcaaatat tatttttagg taacaacaga
    35761 cattttttgc ggcttttagg caaaaaaaaa ccaaaaatag aacattctct tgaaacctta
    35821 cttcgtgagc ttctactttt gtttccttat attaaagccg aaacggacaa tatttcgttt
    35881 ctgccttttc ttcatcagat agtttttgca acttcatata ctacacttat tttctataaa
    35941 agtcaacact tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt
    36001 ttatgttttc aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt
    36061 tagaaaattc attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa
    36121 cacaactgaa taacttttac tttagctact ttagataggt ttgtattcat catgatggat
    36181 acaaacgtta aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa
    36241 agccacgtag acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag
    36301 cattctactt tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat
    36361 ggattttttg tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg
    36421 attaggcgct gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga
    36481 ggcatcagat ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga
    36541 tgctgcctat aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa
    36601 tctaacaatc gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga
    36661 gaaactcgtc aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc
    36721 tttattaaat gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca
    36781 agcccattta gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat
    36841 ggtgccaatc gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat
    36901 cggcatcatg tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa
    36961 agaattgaaa aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt
    37021 ccaacaagtg acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa
    37081 cacgttcgca agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt
    37141 cgttgctggc gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta
    37201 cgaaagcttg ggcaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc
    37261 aagtgaactt cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc
    37321 caaagcatta ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt
    37381 ctcatgacaa atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc
    37441 ctacttgcga ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct
    37501 gaaggaagct ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat
    37561 ccattgacag cttgtcttct aggcttttta gggggtgcgg cagcagggct tgtttccgga
    37621 ctgcttcaca caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt
    37681 ttatattctg tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca
    37741 acgatctttc attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc
    37801 ggtctgcttt ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca
    37861 ggacttgcta ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac
    37921 acagataata tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc
    37981 tccttgcttg cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta
    38041 attggacttg cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc
    38101 cgtctcttga ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc
    38161 gaactgaatg ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt
    38221 ctttctttcc cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt
    38281 gtcaaatgaa tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga
    38341 tcaacgaaaa tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga
    38401 cgatcatcgg tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat
    38461 tttctgtcga tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg
    38521 tcgaacgttc gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc
    38581 gtttaacggt agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct
    38641 tccgtggtgt caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt
    38701 tggggttgga aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag
    38761 ctattacgtt attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca
    38821 ctgcagcgct tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca
    38881 ctgagcagaa tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg
    38941 ggagtcgact gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa
    39001 aacagcagct cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat
    39061 taaaagacga tcaagtactt ttatcttcat aatcagaaag aggatgggac
//
LOCUS       DACEQH010000024        36570 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.024, whole genome shotgun sequence.
ACCESSION   DACEQH010000024 DACEQH010000000
VERSION     DACEQH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.024"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IW833_001274"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IW833_001274"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ1307403.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IW833_001275"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IW833_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ1307404.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IW833_001276"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IW833_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ1307405.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IW833_001277"
     CDS             complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IW833_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ1307406.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IW833_001278"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IW833_001278"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ1307407.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IW833_001279"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IW833_001279"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ1307408.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="IW833_001280"
     CDS             complement(8054..8524)
                     /locus_tag="IW833_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ1307409.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="IW833_001281"
     CDS             complement(8570..8995)
                     /locus_tag="IW833_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ1307410.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="IW833_001282"
     CDS             complement(9062..9331)
                     /locus_tag="IW833_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ1307411.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="IW833_001283"
     CDS             complement(9342..10937)
                     /locus_tag="IW833_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1307412.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IW833_001284"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IW833_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ1307413.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="IW833_001285"
     CDS             complement(14490..15050)
                     /locus_tag="IW833_001285"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ1307414.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="IW833_001286"
     CDS             15403..16377
                     /locus_tag="IW833_001286"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1307415.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="IW833_001287"
     CDS             16547..17947
                     /locus_tag="IW833_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ1307416.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="IW833_001288"
     CDS             complement(18025..18747)
                     /locus_tag="IW833_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1307417.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="IW833_001289"
     CDS             complement(18826..19332)
                     /locus_tag="IW833_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1307418.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="IW833_001290"
     CDS             19455..21254
                     /locus_tag="IW833_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ1307419.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22896)
                     /locus_tag="IW833_001291"
     CDS             complement(21322..22896)
                     /locus_tag="IW833_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307420.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="IW833_001292"
     CDS             complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="IW833_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ1307421.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="IW833_001293"
     CDS             complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="IW833_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ1307422.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24673..25311)
                     /locus_tag="IW833_001294"
     CDS             complement(24673..25311)
                     /locus_tag="IW833_001294"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ1307423.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25312..25962)
                     /locus_tag="IW833_001295"
     CDS             complement(25312..25962)
                     /locus_tag="IW833_001295"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1307424.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="IW833_001296"
     CDS             complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="IW833_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ1307425.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="IW833_001297"
     CDS             complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="IW833_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ1307426.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29105..29845)
                     /locus_tag="IW833_001298"
     CDS             complement(29105..29845)
                     /locus_tag="IW833_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ1307427.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="IW833_001299"
     CDS             complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="IW833_001299"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ1307428.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31216..32154)
                     /locus_tag="IW833_001300"
     CDS             complement(31216..32154)
                     /locus_tag="IW833_001300"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ1307429.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32147..32638)
                     /gene="def"
                     /locus_tag="IW833_001301"
     CDS             complement(32147..32638)
                     /gene="def"
                     /locus_tag="IW833_001301"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1307430.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32653..35061)
                     /gene="priA"
                     /locus_tag="IW833_001302"
     CDS             complement(32653..35061)
                     /gene="priA"
                     /locus_tag="IW833_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ1307431.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35314..35619)
                     /locus_tag="IW833_001303"
     CDS             complement(35314..35619)
                     /locus_tag="IW833_001303"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1307432.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="IW833_001304"
     CDS             complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="IW833_001304"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ1307433.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7989 c         6707 g        11988 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt
    21961 tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc
    22021 tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct
    22081 ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc
    22141 tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc
    22201 acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt
    22261 gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg
    22321 tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt
    22381 tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg
    22441 ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc
    22501 atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac
    22561 ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaatac gttcttgtaa
    22621 atctgcaatg tcttttacta attgtgcaga ttcttgacgc aatgtatctt gttttgctaa
    22681 taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga
    22741 ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg
    22801 agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga
    22861 acataccatt actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa
    22921 tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc
    22981 agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg
    23041 acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct agcatttctt
    23101 aagataacaa cgattctttt tcgctttcga cctaaaaata aaaaaagaaa ttattcatca
    23161 ctgaataatt tcttaaaggg aaaaatgaat aatacaaata taatcatgtt caacagcagt
    23221 gtaggtccta aaaaccttgt aataaaatac gtgttattta ctactgctaa tttaaaaatc
    23281 aactggacca ccatcgtaaa caattcataa cccgtgacaa agatgatcat actgaaaaat
    23341 tcagtaaaga tgttgacatg gataacgtct ttcattacat acatcaacca tacaattaaa
    23401 gggagagcta ctgcatagat accgataacg ccaatatagt aagcgtcata gatagccccg
    23461 agaacaatcg tggtaatcag taaataacgt ttttcaaacg ccatactgca acataataat
    23521 gccaatatca gaaagtgggc atttgacata taggtgcctt tcgaccactc ccctagcatt
    23581 cttgttaaat gaccatctat caacatcaaa aagaacaaaa cgattggcag atagtacttc
    23641 attgtttctt tcttcaacat ggactaatcc tccgctagcc gttggacaat cgtcacaact
    23701 gacacgtcat acatttctgc ataaggtttc acataaactt cccgatccag cccataacta
    23761 tctggtttcg ttttaataac cgtaccgatc ggcaagttag ctggagaatt ccctccaaga
    23821 ccggatgtct ggacaacatc cccttctttg atatccgtat caccagttaa ttgggtcacc
    23881 actaaggcat ggagcttttc atcatagttt ttaagcaaac caaacgcttc gccattagcc
    23941 gaagataccc gtactggaaa atgattggag ctttcattag aggatgacag taattcgatt
    24001 ttagacgaag ccgtattgac ctcgatcact cggccaatca atcctttttg cgacatgact
    24061 gccatatttg cttcgatacc gtctttagaa cccttgtcta cgatcagcat gtcctgccaa
    24121 ctgtcaggcg aacgagtaat gacatttgcc gttacttttt cgtaactagt cagcgtctga
    24181 ttcaactcaa gttctttttt caacgcttcg atttctctct cgtaattctt gttttgctgt
    24241 gaaacgtcat catagctatc gattttttct tttagccgct cattttcggc ataagttgaa
    24301 aaaagattat ggacagaatc tactccattt cgtacccacc gtactggggc agagattgtt
    24361 ttatcaacca ttgcaacact gtcattaacg atggattgga agaaattggg ctttccttca
    24421 ttcgctcgct gcgcagcggt cacacttagt atcgtcacca cgatgataac aataaccaag
    24481 gtaataataa tatttttatt aggattgaac ttcttcacaa agacacctcg aattaattag
    24541 actcttgtgt tcaattttat cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta
    24601 agcttttttt tagcaaataa tagattctgt catttttagc ctgaaagaca aattttttta
    24661 tttgtttatg gatcaatctt tgctttgaat aactgcaatc ataccatctg aaaaagaaaa
    24721 ttctgcggta tctgaaagaa attcgttact tgcataagat gtcggatatt ctacttcttt
    24781 attttccaat acatacttgc tgccttttaa tgtaagattc gagacgggag tcaagcagca
    24841 ataagctaaa tacttcatat ctggtatctg tcttactata tgctctccag gcaaataata
    24901 agacaaataa ttttgacaat ctctgataat aagctgatgg gcaaagggtt tgaagcgagg
    24961 ttccatacct aaccagagat tggctaaaag atgatccaat cttccgcccg tagctccaat
    25021 caaagtgact tccgcttgcg ggtaacgttc aaatgttttt aagataccta actgtgtatc
    25081 tgtatcgtct ttttctgcag gggaacgggt atactctttg gcttttgcaa atacttcctt
    25141 ctgctcttcg ttcgacaatg aatcaaaatc gcccactgcc agatctactg ggtagcctgc
    25201 ttgaagcagg tacagcgcac cacggtctat cccgacatag gcatcgtatt tggacagcag
    25261 agactgcggc cagttttgcg gattacctcc agcagctaat aaaattttca tttagtctaa
    25321 tgcctctttt aatcgttcga tttgtccgac tggattttcg gctccataga tatatgatcc
    25381 tgcaacgaag acatccgccc ctgcatcttt acagattttt gctgtttctg gaacgattcc
    25441 gccatctact tcgattgcat aagtccaatt gttctctcta cgtagatcag cgagttccgt
    25501 aattttttct actgtttctt caataaagct ttgtccgccg aacccaggat tgacagtcat
    25561 gactagtact tgatcagcca aaggcaaaac atgtttgatc atggagactg gtgttcctgg
    25621 attgataact actccagctt taactcctaa gctttttgtc atttgaagtg cccgatgaat
    25681 atgaggagtt gcttcgacgt ggacagtaat gatatctgct ccagcttttg caaatgctgg
    25741 aatgtagttt tctggatcaa cgatcatcag atggacgtct aacggtaatt tagtaacagg
    25801 acggattgcc tgaacaatat ttggccctaa tgtgatattt gggacaaatt ggccatccat
    25861 tacatctacg tggatgtaat ctgcgcctcc tttttccact aactcaatgt ctctttgtaa
    25921 attggcaaaa tctgcactca aaatggatgg tgctatcttc attcttgttt cctcctattt
    25981 tcgtttacct tctttttttg catacattgg ttttcttttt tcgatttctt gtaagaattg
    26041 caaataattg tcgtaacgtg tctgtgcgat accacctgtt tctactctat gttttacttc
    26101 gcagccaggt tctttagcat gcatgcactc tctaaatttg caatggacag aagcttctac
    26161 aaattcagga aattgtctcg gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct
    26221 aaatcctggt gtatctgcta ctaacccgcc atagatagga atcagttcca catgacgtgt
    26281 tgtatgccgt cctcttccaa gtgcatcaga aatctctcct gttgctaaat tcagttccgg
    26341 tgatatttga ttcagtagcg tcgattttcc tgctccagac tgacccataa agaccgatag
    26401 ccgttctggg aagtaacgga tcagttctgt cgtatcaccc gtatgattgg gtacaatcac
    26461 cggatagcct attttagtat aggtttgctt gatctcttcg attttttctg ggtcgtctga
    26521 caagtcggtc tttgtcaaaa aaataatcgg ttcgatcgac tcgtattcca aggtaactag
    26581 aaagcggtca agtagattat aagaaaatgc aggctctacg atacttgtga caatgattcc
    26641 aagatcaaca tttgtgactg gtgggcgaac taattgattt tttctcggca agatttccag
    26701 aagatagccg tctgtttgat tttcactctc aaagatcacc tgatctccga caagcggcgt
    26761 gattttccga ttccggaaat ttcctcttcc tcttgtttga taggtctctc cgtttgcgta
    26821 tacatagtaa aaaccgctga ttgcttttct gatttgtcct ttgataagtg ccacctccgt
    26881 ttttgtttat tttaccatac gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa
    26941 gtatatggct tgaaaattga tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat
    27001 caatgtcgca ttcccttctg atatatttta ttcagcactt attttgaact ttctgaggtg
    27061 ctagctgatg aacttgtgga gctggaagat tcttcttttg aagagttgct tgtgctactg
    27121 gagctacttg agctgctaga actgtctgaa gagtcttttt cagaactaga agacgctgga
    27181 tctggtcctt tagagtaaat tacgttcaca accattcctt tggtaattgt cgttcctgcc
    27241 cctggtactg tacgaatcac tttatcttta tccaccgtgt ttgaatattc ggaagtctca
    27301 tgcccgatat attctgcacc gacactagct aaataacttt gtgcttcgct ttttgtatag
    27361 ccggagatat cattcaattt aggctctgtt ccttggctta cgacaaaatt gatgtaagtt
    27421 tcttctgcta caacttcacc cgaggctgga tcctgactga tgatcgaacc tgatggtaca
    27481 gtgtcgctat agtcataagt agcactaata ttattgatat tgaatccttg tgtaacaagc
    27541 caattacgaa cagtttcgta gttttctcct acatgactac tcaaagtcac tgtttctggt
    27601 cctttgctga ctgtgaaagt aatcgtgtct gttttaggat cgacttctgt atcttctgat
    27661 ggagattgcg aaatgatcat tccgctttct acctctgaac tgaattcctc ttctttttga
    27721 atcaagtcag cagaaaaccc taattctttc aaacttgctt cggcatcttc ataactttta
    27781 ccactgtaat catctaattt gatttttttc gtgccgctac taatatacag agtgacttcg
    27841 cgtttttgct tgactgtcgt tcccgctgcc gggtctgtct tcactacatt accttcttcg
    27901 attttgtcat ctgcaatttc ttctgttttt gttgctactt ctaatttcgc ttttgcgagt
    27961 gtttctctag cagaagcttc agaaagatca gaaacatcag gaacaacaac ttctcctcgc
    28021 ccgccagaag caaagaatac agctgttccg attcctaaaa cagcaagaat agctaatagg
    28081 atcagccacc attttttctg tcctttcttt ttaggatcag gcagaacttc ttcctcctct
    28141 ggcaaggttt cgttttctgg cttgtctttt ggcagaggca tagatttgaa tgaatcaggc
    28201 attggtgcat cttccgtaag gggtgtgata acttttgttt cattatccat cacatgaggc
    28261 tgccattttt cttcgtttgc acgagctggt gataatactg tagaaagatc atcagacatt
    28321 tcctcggctg acttataacg gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt
    28381 tccagtgctt gtggcacatt tggatcaagt gcttttatag atggaagatc atcttgaaaa
    28441 tgttttaaag caattgttac agcagattca ccatcaaacg gtacgctacc cgtcaacatc
    28501 tcataaagga tgatccctaa agcataaata tctgactgct tcgttgccat acttcctctg
    28561 gcctgctcag gggacagata atgaacagat cccagcatcg tattcgtctg cgtgatcgat
    28621 gtttcagata aagcgatagc aatcccgaaa tccgtgattt ttacaacacc ttcattatcg
    28681 atcagtacgt tttggggttt caaatctcgg tggatgattt gatggttatg agctaaagaa
    28741 atagcagaaa ggatctgctg cataatattt actgctgttt cataaggaac aggataatgt
    28801 gtttggatat atcgtttcaa gtccattcct ttgacgtatt ccatcaccag atactgcatg
    28861 ttgtcttctt caccaacatc gtaaacacta acaatgtttg gatgaacgag ttctgttgct
    28921 gctaaagctt ctctctgaaa tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt
    28981 aagactttaa ctgctacatc acgatccaag attaaatcgt gcgccaaaaa tacatttgcc
    29041 ataccaccgc taccgatatt tccagtgatt tgataacggc catttaattt tttgcccaac
    29101 tcgatcatac actgtcctcc ttatattcga taagaagaac ggtgatatta tccgctccgc
    29161 ctgcttcatt ggcacgatca atcagctcag ttactttatg agacaatggg ccttcttgat
    29221 tgatgatcgt tcctatttct tcttcagaca gcatgttcgt caagccatct gaacataaca
    29281 gcagtcgatc ttttagttgc caaagatgag tagaaacatc tacttcgact gttccaggca
    29341 tgccgacaga acgtgttaaa atatttttcc ttggatggtt cactgccatt tcttcgctga
    29401 tttctcctga tttgactagt tcattgacta aagaatgatc ctccgtcaat tgcatgattt
    29461 tatcttcacg aatcaaataa gctctgctat ccccaacatt ggcaatcgtg aaccagtcat
    29521 ctgacagagc ggccgctacg attgttgttc ccataccgtt atattccggc tgttcttgtc
    29581 cacgctgata aatcagcata ttttcttctt gaatcgattg aatgaaccat tgggcgattt
    29641 tttcatcgtc aacagcttct tgtttttccc aagcttcgcc tagattcgtt accgccatct
    29701 gactggcaat gtctccggca cgatgaccac ccatcccgtc tgctaataca gcaagcttga
    29761 gacctgaacg gctggtaaat acactggcat agtcttgatt tgtgttacgt cgtcttccta
    29821 catctgattg atattcaatc tgcatttttt cacctcatta ctttttgcgc aaacaacaaa
    29881 tgaaaaatcc atctgtgtaa aactgatgcg gatacaaagt caacattttc tcttcgattg
    29941 atgattgtag cataggattg gtcaaaacat caatcctctc aaaatcaggg tgtctttcca
    30001 agaaagcaga aacaacgtct tggttttctt cttttaagat cgtacaagtg ctataagtca
    30061 gaataccgga agactttaaa gttggtgcgc agctttctaa aatttccaat tggatttttg
    30121 gtaaattcgc taattcatcc ggctgttttt tataacgaat atctggtttt cgccgaagca
    30181 gcccaagacc tgaacaaggc gcgtctacta aaatccgatc aaactgctct gcttcgaact
    30241 gtttactgac ttctctagca tccatctttt gagcgtagac agcagaagag acacctagac
    30301 gtgctgcatt atcttcaatg agtttgactt tgtgttcgtg tacatctaaa gcgaccactt
    30361 ctccaccgca tgacggatct aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg
    30421 cacaggcatc taagacatga tgttcaggct cgatttgcat agacggcgct acaagcatcg
    30481 aactttcatc ttggatcgtc atgacgccat cgtggaacaa tgagctagaa gctaaatgcc
    30541 cttttttcgc tactataccg tatggactga cttggctttt ttcagctgtg atctcttctt
    30601 cttgcaaaac agtgatggcc tgatctctgg aaagtcggcg tgtatctact cgggcactag
    30661 catggctagg ctgatatagc gataaaccga gcttttcagt ttcttcgtat ccgatttgat
    30721 ccaccagtcg tttcgtcatc catagaggca ggctgatttt gacagaaagt ctttcgatag
    30781 gatcagcaat gccttgcaga tctggtgctc cttgacgctg ataattcctc aagacaccat
    30841 ttacaaattt ccctgtccca ggatttcctt ttacttttgc gatttcgacc gcctcgttta
    30901 aaatagcatg tgcagggact ttatctaaaa attctaattg gtatacagaa agataaagaa
    30961 gcgtcttgac ccagtcatcc actttttttg ctttagcgat caatggtgca acgtaaaatt
    31021 ccaatagtcg ctgatggcta atcgtaccat agatcaattc tgtaaataaa cccacatctt
    31081 tttgatttag ctcgctttgt ttgatcatct cattcaacaa aagattcgag taggcgccac
    31141 ccttgttcac tcgctctaat gtcagtaatg ccatatatcg tactgatttt ttcatttttt
    31201 tagggatttt tttcattatt tcacctgctg accaatctga acttgctgac cgcttccgtt
    31261 aaggaaatca tgtacggcct gttttccttt accagctggc tgtaattcat tgatggctaa
    31321 gacggtctgt ttaccgcaag caatccatag attttttttg tctttccgga tgatcgtacc
    31381 aggtgctttg tctgtagttt ctgccaatac ttcaaccgat aataatttcc agcgtatgcc
    31441 ctcataggtc gtgaatgcaa ttggccaagg acgcattccg cgtacttggt tgtcaatttc
    31501 ttctgcagtt ttatgccaat cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt
    31561 tgcttctgac tcgtcttgag gtactggttt ttgtccatcc aaaatattgg gcaatgtttc
    31621 tagcaatagc tttcttccta aaaggctaag cttttcaaac atcgtcccta catcatcttg
    31681 tttagtgatt ggtatgcttt cttgcgcata gattccgcct gcatccattt ttttgatcat
    31741 ttccatgatc gttacaccag tttctttctc cccattcatg atggcataat gaacaggcgc
    31801 acctccccga tatttaggaa gtaaagaagc atggacattg attgccccta attttggtac
    31861 ttgcaataac ttttcaggca agaattggcc aaatgccgca gtgacgatca catcaggcac
    31921 caattcaatg atttcttcca tttctggcga accggagatt ttctctggct gcagcacacg
    31981 gataccgtgc ttcagagccg cttcttttac cggtgtcggc gtaattactc gtttccgccc
    32041 aactggacgg tcgggctgtg ttaccactgc ctggatctcg taacctgatt ctatcagccc
    32101 ttcaagaatc ggcacagaaa aagcaggtgt gcccataaat actagcttag tcatcaagat
    32161 gttcctccat atattcttca agttcttctt ccgggatctg ctgtatcatt ttctcaatga
    32221 acaagatacc gtctaagtga tcgatttcat gctggaaggc acgggctaaa tagccaaaag
    32281 ccgtcacttc tatctcttct ccgtctcgat cataataacg cacggtcact tcatctgctc
    32341 gcttgaccgt tccatagaca tgcggaatac tgagacaacc ttctacgtca aggctttcac
    32401 cttttgcttc gatgatttct gggttaatca gttcaaattt ctcgccttca tctacttcga
    32461 tcacagcaat tctcttgttt tgtcctactt gaggagcagc gatccctatt ccgtcattcg
    32521 caatcattgt ctcatataaa ttgtctaata aagcgattgt ctcgtccgtg attacatcga
    32581 ttggctgagc agttcttttt aatttatcat tagggtggat caatatagga tagcgcataa
    32641 tttggccctt tcttaaataa agtttaatgg ttcgttgtca attgccacaa aaagaccttg
    32701 tcgctgttct ttctggcttc cattcaaaat ttcatccaaa acatgaggaa gattcggttc
    32761 atgtttatat ttgatgatta gttgataata atatcggttt ttcactctca aaatcatgct
    32821 gggcgttggc cctaatagca agctttgagg gcttaacact tcttttagct gattggcaat
    32881 ctgaaagatt ttctttgccg ctacttgttc ttcaggatgg ctggcggtta ttttcacagt
    32941 aaagtaataa ggaggatagc ctccacgatg ccgtacatgc atttcctgct gataaaactg
    33001 ctcataattt tgttttttag ctaattctat ggcgtaatgt tgaggattga atgtttggat
    33061 cacgacttcg cctgcttttt ccgctcgccc tgctcgtcct gaaacttggg taagcaattg
    33121 gaatgtatgc tcactagaac gaaaatccgg taaattcagg gaggtatcag cattcaacac
    33181 cccaactaat gtcacttctg gaaagtccaa acctttagca atcatttgtg tccctaataa
    33241 tatatctgct tcttttgcac cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg
    33301 agtggtatct acatccatac gcaaaatcct agcttgcgga taaagttctc ggagttcctc
    33361 ttctactttt tgagtacctg ttccatagta gcggatttta ttgccgccac aattaggaca
    33421 gcgattggga ataggttctt catgtccgca ataatggcag cgcattgaac gtgtatccat
    33481 atgcagtgta agagagatat cgcaattagg acatggaaga acaaatccac agtccctaca
    33541 catcacaaaa gacgaatatc ctcgacgatt gagcagtagg acagtttgtt cttttttgtc
    33601 taatcgattt tgtatttttt cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg
    33661 aaactcttca cgcatatcta cgatctcaat agctggaagc tgtgcagctg ctttggctcg
    33721 gtggtttaac tgcaataact gataaacgcc tttttgagct cgtgctcttg attctaaaga
    33781 gggcgtcgcg cttcctagaa ctattgggca atgatgatat ttactgcgcc aaattgctag
    33841 atctctcgca tgataacgcg gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc
    33901 gataatgatc agcccgatat ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac
    33961 tacttgcgct tcttcccgct caatcttgcg ccattcatca tatttttcac cttgggaaag
    34021 tccgctatgt aagaccgcaa ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg
    34081 cggagtcaat gcgatttcag gcactaacat aatagccgtt ttattttctg acaaaacatc
    34141 agcgattgcc tgaagatata cttctgtttt cccgctgcca gtgattccct ccagcaaaaa
    34201 gacctgatct ttttgctgct gacctgctgt gatgatttgt tcgacagcat ttttttgttc
    34261 tgcattcagt tccaatgccg tcgtttggtc aaatactcgg tctttataag ggtcgcgata
    34321 acgttctgat tctataaact ccagccagcc cttttctttt ccttgattca agacagcagt
    34381 actgattcct ttatttttga aataagcaat tggttcttct tggctcagtt cagataaaca
    34441 caaaagaagt tctttttgtt tcttggcatt ttgccgtaat tttgcccatt cttcttcgat
    34501 ttgcttttca gttaacaaag aacggaccaa acgaatcgtt tttactttat ttcgtgtatg
    34561 gacttcatat ttgatatcta ctaattgctc ttggcgccag cgaatcaatt gagaaaggct
    34621 gccttcttct tctgcttcct tccaggaaat cacttctcgc tgatcaaaca atacctgaac
    34681 ttctggtgtg tctgccaaag gatagatcaa tttatcatat tctgctttca tcacactcgg
    34741 aagcatcgtt tgtagacaag tgattttgaa tgcgtaagta atttttttca tataatcagc
    34801 taatgaaagt aattcttcat tgaccaccgg agcaaggtct agcaaacgaa taactgcctt
    34861 caaagatgga ttagctgatt cttctgattt ttggatagcc ataacaaatc cttggacatg
    34921 acgattgcca ttaccaaaag ggacttctac tcgcatcccg acttgcaaag ctgtttccat
    34981 ttcagaggga accaaataag tgaatggctg atcggtctgc atagtaggta cgtccacaat
    35041 gacttcagct gttaatgtca tggttcgttt cctccttttt ttgtcttttc ttatcaagcg
    35101 acaaaaagtt ccttgcctat tttactagag atcgatggat ttgtcttatc ttctgccaaa
    35161 tcctaacaaa aaattaacag cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca
    35221 cagccttttc taccagacaa taagtggcga atgtttgaaa aggaaacagc tgggtttgct
    35281 tttcttttta gatgttcgct ctatttttct ttattacatt ttttgctctt cacggatacg
    35341 tgattccaat tcgtgttgtt cacgctcttt ttgtgcttga cgttcctctt cttccatctt
    35401 caaacgttca cgttttaatt caggatgtgg atcattgact acatttcctg catcgatttc
    35461 ttctaaagct tgaccgacgc tttttactga atcaaaagaa tctagagtag gctgtgcact
    35521 tgcatccaat tcatgcgcgc gtttacttgc taaaatcact agagaatatt ttgaatttac
    35581 acgatctaat agtgagtcaa ttgaaggttt taacatcata agactacatc tcctttaaca
    35641 ttttaatata tttaccgatt acgcgatcta ctcgaaaatg ttcactggca ataatgtttt
    35701 tgattcgttc aactgctagc ggcacttcat catttacaac agcatagtcg taaagagcca
    35761 tcatttcgat ttcttctttt gcaactttca ttcgttcttc gattacttca tctgcatctg
    35821 ttcctcggcc tacgatccgt gattttaatt ccgctaaatc tggcggagtc aaaaagatga
    35881 atacaccatc gggcactttt tctttgactt gttttgctcc ttggacttca atctccaaga
    35941 aaacatcttt tccttcatcc aatgtcttgt tgatatatga caaaggagtt ccataataat
    36001 tcccgacata ttctgcgtat tccagcatct ctccagcttc gatcattgat tcaaactctt
    36061 ctttgcttcg gaagaaataa tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag
    36121 tcattgagat cgaatactga aaatcattat cctcactctc aaaaattgct tttcgtactg
    36181 tgccttttcc tacaccggat ggaccagaca acacgattaa taatccacgc tctgacatga
    36241 cgacgtcctt tcaactagtc tttttgtact tatcgtttat tttgcacttt ttttctgcaa
    36301 ctttcaaggt tatctttaat tatagctaga aattggtaaa aaagctaccc tgtaccaaga
    36361 ggtcgtaaaa aagctgtcct gcatcaagta ataagaacag ccaaacataa acagcttctc
    36421 atgtttttcg tttggttggg gttattatcg cagatgcaag cttttgaacg ccgtgtataa
    36481 gaggtcgtga agaagccgat tagtttcgag tatgaagaaa aattttgtga aaatagtttc
    36541 tcacattttt gcataaattt ggcttaatac
//
LOCUS       DACEQH010000025        34284 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.025, whole genome shotgun sequence.
ACCESSION   DACEQH010000025 DACEQH010000000
VERSION     DACEQH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.025"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            33..1082
                     /locus_tag="IW833_001305"
     CDS             33..1082
                     /locus_tag="IW833_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1307434.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            1079..1960
                     /locus_tag="IW833_001306"
     CDS             1079..1960
                     /locus_tag="IW833_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1307435.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            1963..2733
                     /locus_tag="IW833_001307"
     CDS             1963..2733
                     /locus_tag="IW833_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1307436.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            3012..3866
                     /gene="aroE"
                     /locus_tag="IW833_001308"
     CDS             3012..3866
                     /gene="aroE"
                     /locus_tag="IW833_001308"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ1307437.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            4044..5207
                     /locus_tag="IW833_001309"
     CDS             4044..5207
                     /locus_tag="IW833_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1307438.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            5324..5734
                     /locus_tag="IW833_001310"
     CDS             5324..5734
                     /locus_tag="IW833_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1307439.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            5908..8136
                     /gene="topB"
                     /locus_tag="IW833_001311"
     CDS             5908..8136
                     /gene="topB"
                     /locus_tag="IW833_001311"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1307440.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            8274..8591
                     /locus_tag="IW833_001312"
     CDS             8274..8591
                     /locus_tag="IW833_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307441.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            8751..8987
                     /locus_tag="IW833_001313"
     CDS             8751..8987
                     /locus_tag="IW833_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1307442.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            8984..9382
                     /locus_tag="IW833_001314"
     CDS             8984..9382
                     /locus_tag="IW833_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ1307443.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(10049..10993)
                     /locus_tag="IW833_001315"
     CDS             complement(10049..10993)
                     /locus_tag="IW833_001315"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1307444.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(11289..12233)
                     /locus_tag="IW833_001316"
     CDS             complement(11289..12233)
                     /locus_tag="IW833_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1307445.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            12454..13668
                     /locus_tag="IW833_001317"
     CDS             12454..13668
                     /locus_tag="IW833_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1307446.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            13690..15705
                     /locus_tag="IW833_001318"
     CDS             13690..15705
                     /locus_tag="IW833_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ1307447.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(15788..17161)
                     /locus_tag="IW833_001319"
     CDS             complement(15788..17161)
                     /locus_tag="IW833_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1307448.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(17187..17471)
                     /locus_tag="IW833_001320"
     CDS             complement(17187..17471)
                     /locus_tag="IW833_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1307449.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(17473..19488)
                     /locus_tag="IW833_001321"
     CDS             complement(17473..19488)
                     /locus_tag="IW833_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1307450.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(19667..19972)
                     /locus_tag="IW833_001322"
     CDS             complement(19667..19972)
                     /locus_tag="IW833_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ1307451.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(20061..21092)
                     /locus_tag="IW833_001323"
     CDS             complement(20061..21092)
                     /locus_tag="IW833_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ1307452.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            21299..22174
                     /locus_tag="IW833_001324"
     CDS             21299..22174
                     /locus_tag="IW833_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1307453.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(22326..23168)
                     /locus_tag="IW833_001325"
     CDS             complement(22326..23168)
                     /locus_tag="IW833_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1307454.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(23260..25137)
                     /locus_tag="IW833_001326"
     CDS             complement(23260..25137)
                     /locus_tag="IW833_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1307455.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(25134..26555)
                     /locus_tag="IW833_001327"
     CDS             complement(25134..26555)
                     /locus_tag="IW833_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1307456.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(26558..29947)
                     /locus_tag="IW833_001328"
     CDS             complement(26558..29947)
                     /locus_tag="IW833_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1307457.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            30204..31595
                     /locus_tag="IW833_001329"
     CDS             30204..31595
                     /locus_tag="IW833_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1307458.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(31668..32315)
                     /locus_tag="IW833_001330"
     CDS             complement(31668..32315)
                     /locus_tag="IW833_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1307459.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(32323..33009)
                     /locus_tag="IW833_001331"
     CDS             complement(32323..33009)
                     /locus_tag="IW833_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1307460.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(33015..34088)
                     /locus_tag="IW833_001332"
     CDS             complement(33015..34088)
                     /locus_tag="IW833_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1307461.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
BASE COUNT        10502 a         6288 c         6493 g        11001 t
ORIGIN      
        1 aataaaaaga ttgactttgc aggaggaaaa aaatgaaaaa caaacgatta ataacagttg
       61 tagtaattat tttagtttat ttagcaggtg catttattta tggaaaagcc tcaggcgaaa
      121 aagaaagtac ttcgacgaac cagacaaaaa aaacaacagt tgttggggta ttgcaatacg
      181 tgagccatcc ggcattagat gagatttatc gaggaatcaa agatggtctg aaagaagaag
      241 gattaaaaga aggagaaaat cttgagatca aatttcaaaa tggacaagcc gatcagagca
      301 agctagccac aatgagtcaa cagctagtac aatctgatcc agatgccttg attggaatcg
      361 caacacctgc cgctcaatca ttagcaaaca cgacttcatc gatcccagtt gtgttaggtg
      421 ctgtaacgga tcctgtgggg gctggattag tcgcttcttt agagaaacct ggtggtaatg
      481 taacaggtgt ttctgatcag ccgcctgtta cttcacaaat caagcttgct tctgatttgt
      541 tgccaaatgc aaaaaaagtc ggaatcttgt atgcttcttc agaggataat tcaaaatacc
      601 aagtagcaca agcagaagaa gctgcaaaaa aagaagggtt agaatcagta aaatatgcgg
      661 tgccttccac taatgagatt gcacaaacgg ttcaagtgat gagccgacaa gtcgatttta
      721 tctatgttcc attagataat accattgcca atgcgatgca aacagtggta aaagaagcaa
      781 acaaagcaaa tatccccgtc attccatctg tggatacaat ggtggaacaa ggtggactag
      841 caacaatcgg gatcaatcaa tatcaattag ggcttcaaag cggtaagatg gcggcagaac
      901 ttgcgagtgg aaaagaaaaa ccggaaacga cgcctgttta tacatttgat caaggggata
      961 cagtgatcaa tcaatctcaa gctgatcgct taggaatcat tgttccccaa tcgatgaagg
     1021 aaaaagccaa aatcattaca gatgactcgc agcaagaaac atctaaggag gatgacaaat
     1081 gatcgtatca gctatttcac aaggcatgct ttgggcaata ttaggattag ggattttcat
     1141 gacttatcgg atcttagact ttcctgatat gacgacagaa gggtccttcc cattaggtgc
     1201 tgctgtctgt gtgacagcta ttactcatgg ggtagcgccg attgtagcta cattattagg
     1261 cgtaggtgct ggcatgatag caggactcgt tacggggtta ctttatacaa aaggaaagat
     1321 accggttatt ttagcaggga ttttagtgat gtcaggattg aactcagtta ttttgtttgt
     1381 gatgcgaaca ccaaatcttt ctttattgaa tcatccgatt ctacaagaca gcttccatcg
     1441 ctttgggtta cctgattact ttgacacagt ccttcttggc ttcatctctt tgggcatctt
     1501 gattgctgta ctgctattct tcttcaatac agatttgggt caaggctata ttgcgacagg
     1561 agacaacgaa acgatggctc ggtcgttagg gataaaaacc gatcgtatga aaattttagg
     1621 tttaactttg tcgaacggtg tcattgcatt atccggtgcg ctgattgccc agaacgacgg
     1681 atatgcagat gtaaataaag ggattggcgt aatcgtcatc ggtcttgctt ccatcatcat
     1741 cggtgaagtg atcttccaag aattgacgct agcagaaaga ttgattgcta ttgttgtcgg
     1801 cagtatcatt tatcagctat tgattttagt agtaatcaaa ctagggtttg acacgactta
     1861 tctgaagttg ttctccgcta tcatcttagc tatttgtctg atgatcccgc aattgaagaa
     1921 agcactgaac ttaaaaacgg gttttgagaa ggaggcatag taatgactga tatattgaca
     1981 atcaaaaatg gcagaaaagt cgtaaacaat ggaatgaatg aagagaaagt actattggat
     2041 gaaattaacc ttacaatcag agaaggggac ttcatcactg tccttggcgg gaatggtgct
     2101 ggaaaaagca cactgttcaa tacgatcgct ggtacgcttc aactcagtca aggatcgatc
     2161 tcttttaaag atcagctgat cacaaaagat tcagaagaaa aaagagcacg attcctttct
     2221 cgagttttcc aagaccccaa gatgggaacg gctcctcgga tgacagtagc agaaaatttg
     2281 ctacttgcac aaaaaagagg tcaaaaacgg aaattccgtt tacgtaactt gaaaaatcaa
     2341 aaagaagatt ttttcagact atgtcaagaa gtcggtaatg gcctagagca acacttggat
     2401 actccagcgg gtgaattatc tggtggtcaa cgccaagcat taagcttgct gatggcgacg
     2461 atcacagctc ctgagttact attattagat gaacatactg cagctttaga tcctaaaaca
     2521 gctaaagcat taatgcgatt aacaaatcag cggatcaatg aacaaaaatt gacttgtctc
     2581 atgatcacgc atcgaatgga agacgccttg aaatacggaa atcgtctgat cgtactccaa
     2641 aaagggaaaa tcgttcaaga tatttctgaa aaagaaaagc aacatttgac aatgggcgac
     2701 cttttgcagt tctttgaaga agtagtggat tgatttcgac agaaaaattc ctttcagtag
     2761 aaggacttga aaactcattg acatcttcct ttgattcatc ctatacttaa aacaataaac
     2821 aattaaacat atttgggtgt tttgtaggat cacgtagtca atgattttaa caattaaata
     2881 gaatattctt tgctctgaca agtaaacgat gagaaaagaa tttgctactt tgccacctag
     2941 acgtttcgta ctttttcgta tcaaacgcta agtggctttt tttattgaag taagaacaaa
     3001 ggaggaataa gatgatttct ggacaaacca aattagctgg gctctttgct gatccggctt
     3061 ctcacagttt atccccattg atgcacaata ctgcttttga agcaaacaac atcgatgcgg
     3121 tgtatttagc tttccaagta aatccaatga atttgaagca agcagtcgaa agtattcgaa
     3181 cttttgatat gttaggtgta aatctttcga tgccgaataa aacagcggtt attccttatc
     3241 tggatgaggt aagccaggaa gcgcaattga tcggagcagt gaatacgatc gtgcacgaaa
     3301 agggtcgttt gattggctat aatactgatg gtgcgggctt catgcaatcc gtgcaagaag
     3361 ctgggatttc gatcaaaaag aaaaaaatga ctctactggg agctggaggt gcagcaaagg
     3421 cagtcactgt tcaagcagcg ttagacggcg ctgaggaaat cgtagtttat aaacgaaaca
     3481 accaaacatt cgctcaagta aaagaaacat tcgaacaact cagctatcga acttcttgta
     3541 agataaaagt tcgtgacttt gccgatacgc atcaattaag aaaagacttg aaagaaagct
     3601 gtcttctagt caatgcaaca gatatcggga tgggaaagaa aatcaatcag tcaccgctct
     3661 tagatataag catgatgcat gagaatctag cggtttttga tttgatttat gcacctagag
     3721 aaacacgttt attaaaagaa gcaaaaaaag taggagcaaa aggatacaat ggacttggca
     3781 tgttgatcta ccaaggagca atcgcgtttg aattatggac aaaggaaaag atgccgatcc
     3841 atgtgattga aaagctgttt gattaaaaaa ggataataga taaagatctt ttattctcgc
     3901 ataaacggtc tcaagaaaag taatccttta tgtatcgaag catttatttc gttatttttt
     3961 attaaattat caaaaaatga taggcttttg tcgattggtt atcgtatcct ttatttatca
     4021 gtaagaaacg aggaggaacc acaatgagtc ggatggatcg ctataaggac atccataaga
     4081 aggcgaaacc tctgaagcgg gatacgggga gtatttttgc tagaagagaa cgaaaacgaa
     4141 cgcgtatgga aaatgaggaa gatgctaccc gtatttatga aagagagcag cattcatttg
     4201 aacatttgaa ccaatacgag caacagatat acagagatgc accaagagca gaagaacaac
     4261 aaaaaaataa aaaatttttc aatcggcaaa agaagaacaa accgttaaaa cctcgaagaa
     4321 agcgatcgtg gaaaaaaatc attctatcga ttttgcttgt tttgatcctt tattcaggta
     4381 tctcattttt tattggtaaa tatgtagctg aacatgacac aagcttagag gcagcgactt
     4441 cagaaacctt tcatgggcaa aaagcgacga atggtgcgca taatattttg atccttggaa
     4501 gtgatacacg cggagaagat gccgggcgag ccgatacgat catggtctta cagttggatg
     4561 gtccttccca taagccgaaa ctgatttctt ttatgcgtga cagcttcgtt acgatcccag
     4621 gggtaggaca aaataagatc aattcagctt atgcatacgg aggagccgat ctagtgcgtc
     4681 aaactttagt agaaaatttt ggaatcgatt gtcaatacta tgcgaaagtc gatttcaaat
     4741 cgtttgaaaa agtgatcgat gccttattca tgaacggagt gaagatcgat gcagaaaaag
     4801 atctgaatct ggatggtgta gatattaaaa aagggggcca gaagatggat ggacatgttc
     4861 ttttgcagta cgcacgtttc cgaatggatg aacaaggaga ctttggccgt gtcagacgcc
     4921 aacagcaagt aatgaacgct attttcagcc aattgaagaa tccattgaat ctgatccgtg
     4981 caccttatgc agctggtaaa gctttaggat atacatccac agatgtcact gcttggttct
     5041 tgacgaaaaa cctcttttcg attgcacgag gagctggtgg cattgatcga ataagtgtgc
     5101 cagtagacgg ttcgtggaat tatgggcaaa gtgattatgc tggcagtatt cttgaaattg
     5161 ataatcaaat gaatcaatct gctattacag attttttgaa taaatgatca taaaaaagat
     5221 caagaagaag ctgcgcaagg cttttgtcat gaatcaatca aacgattcac tcgtaaaaaa
     5281 cgctttctta tggtagattt aaccaagaga ggagctgttt acattggata tctatctgaa
     5341 cttcaaaaat gagtcgcgtg aagccatcgc tttttatgaa aaagtttttc agactgaagc
     5401 atcaggtatc accacatacg gtgaaatgcc tggaatagaa caaatgaact tgccagaaga
     5461 agcaaaaaat ctaatcacga atgcacggct gaacatttat gatacattag ttatgtttgc
     5521 agatacgcca gaaggaattg gtcctgaatt agttgtggga aataatgttt ctttagtcat
     5581 ccagacaaga aatgaagaag aaacgaagcg cctatttgaa agcttagctg agggtggtaa
     5641 gattgagatg ccactggatg ctgttccgtg ggctcagttg tatgggatgg taactgatcg
     5701 atttggcatc aactggcaga ttaaccaaga gtgacagcta aagatgaata ttttctggaa
     5761 acatcaagca gcaaacggat catcgtttgt ttgcttgatg tttttgtgtc tatttcgact
     5821 atacggtatc ttttttttat tgccagatgc gaaaatatgt tccataatag agaagtccgc
     5881 aaaaaattaa gtaacggagg tcagaagatg aagacggtga tactagctga aaaaccttca
     5941 caagcaaaag cctatgccga aaccttccaa cagcaaaagc ggcatgaagg gtattatgaa
     6001 atcagcgatc cattgtttgc tggtgaagta accatcactt atggttttgg acatttggta
     6061 gatatggttg ctccaggagc ttatgaagag cgttgggcaa aatggtcttt agaaaatttg
     6121 cctattttcc cagaaacctt ccgttacgaa gttccaatgg acaaacgtgc acaattttcg
     6181 atcgtaaaac gagaattgca gtcagccgat accatcatta ttgcaacaga tggtgatcga
     6241 gaaggtgaag ccatcgcgtg gtcgattatt cgacaagcaa acgcattttc taaggaaaag
     6301 aactatttac gtttatggat caattctctt gaaaaagaag caatctatga aggttttaag
     6361 aatctccaac ctggaaagaa ctattttcca aaatataaag aagcgcaagc aaggcagaac
     6421 gctgattggc tgattggaat gaatggaagt ccattatata gcttgctact tcaacgaaaa
     6481 gggattgatg gcagtttttc tcttggtcgt gtacaaacac cgacactcta tatgatttat
     6541 cagctgcaag aagaaatcaa acacttcaaa aaagaaacgt actttgaagg agaaggttta
     6601 attacgacaa acaaagggca gttttctgga aaaatcgtac caaaaaaagc atttaaaacc
     6661 caagaagagt taacgaagga aatagaaaaa cttggagcgc atttaggtaa gcaaaacgga
     6721 cggattcttg aagttacgaa aaaggaaaag aggttgcata gtccacgttt attttcgcta
     6781 tctagtttgc agacaaagat gaatcagcta atgaaagcaa gtgccaaagc tacacttgat
     6841 gctgctcaag gattgtacga aagcaagttt ctaagctatc caagaacgga ttccttttac
     6901 attacagaaa atgagcatca gtatctggtt aaacatctgc aaaattataa aaaattttta
     6961 ggaatagaag aagtacagac aactgagatc aaagcaaaga aacgatatgt cgatgcgaaa
     7021 aaagttcaag aacatcatgc aattattccg acaaagacga ttccgactcc tgcacgcttt
     7081 gcgaagcttt ccaaattaca gcaagcaatc tatttgcaag ttttgcgcac tactgtggct
     7141 atgtttgctg cagattatat ctacgaagag acgaccgtaa tgacgggcgt acagcaatta
     7201 aagctaaaaa gcatcggaaa gatcccatta cagcaaggtt ggcaggaaat cttacgcatc
     7261 aagtcaaaaa agaaagaagc tcagactctt cctcctatcg tagaaaatga agaagcagaa
     7321 gtagaactga aaagtatgga gaaagaaaca caacctccaa aaccctatac cgaaggaacg
     7381 ttgatcacag caatgaaaac tgcgggaaaa acgctggata atgatgaagc gcaagaaatt
     7441 ctcaaagaag tagaaggaat agggacagaa gctacacgag ccaatatcat tgaaaatctt
     7501 aagcagaagc attacatcga agtcaataaa aacgaaataa ctgttacgcc aaaaggaatc
     7561 actctttgta aggcagtagc caatgaacca ttactgacga gcgcagaaat gactgcgcgc
     7621 tgggaagggt acttgagaaa aattggtaaa caagagggga caccatctgt ttttttagag
     7681 aatatcaaaa aattcatcct acatttattg gcagaagttc ctaatcaaat cgacaaggta
     7741 gacttttcag atgagattag cgggacacgt cagataaaag cgatggagaa gaaaaacgat
     7801 caattaggaa gatgtccaaa atgtaaaaag ggaatagtca tgctttatcc taaaatcgct
     7861 acatgcttga atccggaatg cgacttcaaa ttgtggccaa cagtagctaa aaagaagctg
     7921 actaaaacga atctcagaga tttgctttca aaaggaaaga caagtaaagt tgtcaaaggt
     7981 tttactggta aaaaagggaa attcgatgca gttttagtat taaaagaaga tatgacaatc
     8041 ggtttttctt ttccatggat cgaaaacagt ggaacaaaag cagttgatgg aaacaaagaa
     8101 acatcaagaa aaaataatga aaacgaagta gagtaaatga aaacaatgaa atagcggaat
     8161 atttattccg aaaagtgaga atataaatac caataaaact aaaaaatatc agtgaatcaa
     8221 gcaaaaacgt tggggtaggc tgattttata tagatagcaa ggagtttttt tatttgaaaa
     8281 aagtgcattc atttttaaat tatcttatgg gagcaatcac gattgtggct gtgtaccaag
     8341 catttctaaa gtatcctgat tacgggacga agttgttgtc attgacgcac aatcaaacag
     8401 catattcacc aacgttgatt ttatcgactt tgactgcagg agctaaaagc gctacttatt
     8461 tttttcataa aacgacaagc atgctaaagc gaaagaaaca gcgaaaacaa ttgttggtgg
     8521 tggtagcggc tatgatcatt atttattttc taccagttat tacacaagta tttcatcaat
     8581 attttggtta agaatacgag agtttgtgac aaaagtcatg tcacaaactt ttttctttgt
     8641 cattttttcg taagaaaaag caaaataaaa gtatatacat tgattaaaag tatatacttg
     8701 gatataatgt acttactata gaatgagaat ggtatagagg gtggcgaaat atgaaaaaaa
     8761 tgaaaaagta taaaattaga aaatctggca atagcgatgt aacaactatc ccaccagaag
     8821 taaaagaatt catgggtgtg cagacaggtg acgcaatcag ttatgttttt caatctgatg
     8881 gttctgtacg aatgatcaaa gcacaagaag aacctgatat tgattcatta gttgattcga
     8941 tcatgaatca atatgaagac gcgttaaaag atttggttga tttatgaggt atctttcgac
     9001 taaagaaatt atcaaaatca acgcgaaagt tattttacgt cactcgccag gcgaaatgat
     9061 cggtgtaaaa gatgcaaatg cattagatat ggccgtcaaa cagccttcac aagcagtatt
     9121 caatcaagaa ctctatcctg aaatctatga aaaagcggcg attttagcaa ttaatttagc
     9181 taaaaaacat ccttttcata atggcaataa acgaactgct cttgttgcca tgttgttgtt
     9241 tcttcaattg aataattgtc cagttgcttt ttctcgtcaa gaagctgttg attttatcat
     9301 aatgattacc actagttcat tggatttcga ttcactaaag tccaagataa ccagttattt
     9361 acgacaaacc gcaataaaat gaacgaatta aaaactttat ttctcaatta gatcagcatg
     9421 tcattaagga atatgctgat ttttttatgt ttaatcatac aaaatagttt tttcataaaa
     9481 atatggaaaa tatggattaa ttttgaaata attgatttaa aaggaataca aaagagtgaa
     9541 tagtaggaaa tagtggttta gaactagtgg gctgattttt tatatatttt agtttacata
     9601 atatatatta tacaaagtag atttaaaaaa tgataaacct ctctcgaacg gttttttatc
     9661 aaaaatcgtc atttttccat caaaatctgc ttatgcaaaa ttataaaaaa attttgcagt
     9721 ttttcttact ttttttgtcg tttaattgaa tgataaaaac cagtttcttt attcagttaa
     9781 tcaaatatga acgaattaga tatgaatcat tgtatttgcc gttgcggaag gattttttaa
     9841 ttaaccacag ataaacattg ataacgattc gcttattgta atcaacagat taaatttcat
     9901 atgttcatat gtacggatac attttcggac cgattaattt taaccattct gtttatttgc
     9961 ttacctatta gatatcaata aaaaactgct taacagctat ttcagctgtt aagcagtttt
    10021 aggattttta gttcatattc attgtttctt ataatacagt gttcaatact tctcttaaag
    10081 cacttgttga tttgctgaac attgcttttt cttcttcgtt gattgataat tcaacaactt
    10141 cacgaacacc attttcgtca acgattgatg gcacacctgt aaagatatct ttttcgtcgt
    10201 attcaccagt taaataagca gatacaggca atacagcttg ttcgttattc aagatagctt
    10261 ttacgatacg tgcagtactc ataccaatac catagtaagt tgctttttta cgatcaatga
    10321 tctcataagc agcattacgg actttatcag caatcacatc taattcatct tgggcaatac
    10381 gatggtcttt ttctacaatt tcaaacactg gtttaccacc gacagttgta tgtgaccatg
    10441 cagcaacttc tgaatcacca tgttcaccta agatatagcc gtggacgctg cgtgggtcaa
    10501 cttttaattt caatgcgatt tctttacgga aacgagttgt atccaatgtt gttccagtac
    10561 cgataacacg tgatgttggc agaccagatt cgttccaagc gatatatgtc aaaatatcta
    10621 ctgggtttga agcaacaacg ataataccgt cgaatcctga tcccatgatt tcttttacga
    10681 tttggcgcat gattgacgca tttgttttaa ctaaatctaa acgagattga ccaggttttt
    10741 ggttgatacc agctgtgata acaacgatat cagcatcttt acattcttca tagccacctg
    10801 accatacagc cacattttca tcgccccaag ccataccatc taaaaggtcc aatgcttccc
    10861 cttctgcttt ttcttgattc acatcgatca atactaattc attagagatt ccttggttga
    10921 tcattgcata agcgatactt gttccaacaa aacctgtacc gacgattact actttacgac
    10981 ttgttttttt catgaataaa cagctccttt tcataactat ttgttacttt catgaaaagt
    11041 atagtcttgt ttattcattt tatcaacatg ttaattataa tagttgttat tataattttt
    11101 gatggaaacg aattcctgaa tacgcataac agataaatga caaatgttta cacctatatc
    11161 ccctgataat acaaccaaaa gtttagaact gaaaataaat ccaaaaaaat tcgctgcaac
    11221 gatataataa gacagcaatc gcacctaaaa caattttcct tcatatgctt tagtgaaaga
    11281 ttttcttctt aatgtttatt tctccattca ttgggagtac atgttgaata cttcttgaat
    11341 aatttgaata aagaagtcgt ttgtttatat ccgatacgat tagcgatttc ttccaacgaa
    11401 aaactcgttt cttgaatcag ttccaacgca aagtcgaatt ttttctttag aaccagttct
    11461 tgaaaggaag catcaaaata cattttcaat ttattactaa gatagaccgg attgtagcca
    11521 aaatgagctg ctgtttcttt taacgtacaa gaagtaaaat gcttatcgat ataatctaat
    11581 actctaagca tctcattttc tgcgcatcca tctgctgatg aaaagctttg ttgttctaat
    11641 aaatgagtca attcgatcag taatagtgat gtatagagat ttaacgaacg gatcttatga
    11701 ctgtatgtat caaaataaat caacagcata ttttcaatca aatgcgtcaa acgacttgtt
    11761 tttccgccgc gaaaatgaat gaattgtgta tgttcccctt tttcttttga tgcatttatc
    11821 aaaaaagaag aaagtagatt atttgtgctt gccaaatgat tcaatatctc tgtagataag
    11881 gtttcttctt ttaataagat gttgattaaa atatcctctt gccctgtgta ttcgattctt
    11941 tgagtagctt ctttatcaat gatcaccacg tcactttctt ccatctctat cctttctcca
    12001 ttaataattt gtaaacatgt accgctatac atataattga tttcaacaaa atcatgtgta
    12061 tgctcgggaa cttccgcata tcgacggtgt ttactgatat gaatatttcc ctgttgaaat
    12121 agatcccctc ggtggatttt tggcggtgtc tgaagatggt aagctaagtt taaatcattg
    12181 atattcattc cttgttcacg ctgcagagtt tcgattgatt cttcgttacg caaaaaaaat
    12241 gtctaattca gctagttcca attgactccc tcctttttgt aagcggtttc tttcgcttat
    12301 tagttcacgc tgtactgcta ttatatgcaa agctattttt ggtttcataa agactttcta
    12361 tggttgggac atgaacttcc tgtgattccg ctattgagag aataaccact tcatactata
    12421 tttttgataa gcgcttacaa aaatgaaagg aacatgctta tgaataaaaa gatgatgaaa
    12481 ttttctctgc tgtcagtctc tcttattttg acttctgctg gtgcagtcac gtctgcaatt
    12541 cccagtatgt cagctactta tgccacgcgt tctttatctg ctgttgaatt attaacgact
    12601 gctccctctt ttatggtcac tatttttgtt ttgattagta gttttattgc taggaaaatc
    12661 ggacaaaaac aaactgtgat catcggatta gtgattgcat cgatttgtgg tatcctcccc
    12721 ctcttctcac aaagttacag tattgtctta gcttcaagag ctggactagg tattggcttt
    12781 ggattgatca attctttggc agtcagcatg attgctgcct tttatcacgg agatgaacga
    12841 gcctctatga ttggttttca aagtgcattt caaggatttg gtgcagcttt aatgacattt
    12901 gttgcaggac agctagtaca gttcggttgg caatatacct ttttagtcta cgccatcagc
    12961 ttacctattc ttgtcttgtt tgttttgttt gttcctacac ctgaaacaac tgaacttcaa
    13021 tcgcatggca cagccccaac aagacaagta atcaaaacga atcctaaaat gattagttat
    13081 ggtatttttc tcttgctggt catcgtggta tataatgctg tatcgattaa attagccaca
    13141 gtcctgacga cttattctct aggaaacgaa actaatgcag cgaatatctt aacaatcatg
    13201 caattggcaa gtatggctgc cggagtttgt tttggctggc ttcagacaaa agcacatcgc
    13261 tatatgctta cactttcttt actactgatg ggaatcggct ttgtattgat tgccactgtg
    13321 ataaacctgt atgtgattgg gtttggtgcg atcctaacag gaatttcttt ttcgttattc
    13381 attccttact tattcaacca agtaagtatc caagcgccca agtacgcgga gagtttcaat
    13441 acttcaatct tattggttgg cgctaattta ggtactttca ttgcaccata tggtttgctc
    13501 tttctctctt ttgcttctct tgggagtaaa acacttgctg ttttcgtcaa tggcagtatc
    13561 ttgctgttga ttagcgcgct tattagcctt ctagtagtac acaggacgaa tagaaagaca
    13621 gtgataagag tagatgaaga aataaaagaa caattgacaa caaaataaat ttgatggagg
    13681 tcgtgtaaaa tgacaaatga aaaacataaa gatagatggc tttggtatcc tggagacttt
    13741 gagatccgcc atggactgct acaaaatttc caacgggaag aacgaggttt tgactggcca
    13801 gcgtactggt acatggatga ttgccatcgg aacgtcaagt ttaagagata ttactttctt
    13861 gatcaacctt ccatgtttaa agtgacgatc caaggagttg ggtatgtaga aattaatgga
    13921 caaaaacatc catgtggaaa atggctgact tgtccagctg gtaaagcaaa aattcgtatc
    13981 tttgtaggac atacatcagg gctaccagcg atgtttatcg agggagacga agtaaaatca
    14041 gatattggct ggacagcaag taattttatt gaagaatatc cagctggctg gagtccgctt
    14101 tatgtcgata tcgcaaaaga tccaaatcaa atctattatc aaaaagaatg cgtccatcca
    14161 gtcactgaaa cggagattgg cggaggaatc ttattcgatt ttgggcgagc agtgaatggc
    14221 tgtgtagaac taatatctct ggatgaccat atcgaatcta tcactctatg ttatggagag
    14281 tccgatgtag aagctatgga tatcgattat tgctactata agcaaatagg aatgacaaga
    14341 aaaatgaaag ctcgtaaacg cgcttttcgc tatttgttta taccagaggt ttctactgga
    14401 atggttcaac ttcaagtttt tcatgaattt ttatctcttt cgaaacaagc tgctttttca
    14461 agcgattctt ctttactgaa tcagatctgg tcagtatcag aagaagcctt caagttatgt
    14521 agtgatttgt tttttatcga cggtatcaaa cgagatcgct ggatttggtc tggagatgcg
    14581 tatcaatcat tcatgatcaa tcaatatctt ttttttgacg aggaaatcaa taaacgaacg
    14641 atattagcat taagaggaca aaatgaaatc aagcagcata tgaatactat cgttgactat
    14701 tcgatcttgt ggttgattgg tatcgaaaac cactatatga tgacgaaaga ctatgaattt
    14761 ttacgcatcg tctaccctaa gatgcaatca ttaatgagat acttgatgga gcaaacgaat
    14821 gaattagggt ttatttatgg gagagcaaaa gattggatat ttattgattg gtctgaaatg
    14881 gataaagaag gaacgcttgc tgcagaacaa gtcttattac tgaaagccta tcattccatg
    14941 atcacttgcg gagatatctt agctgaaaca tatggagaga acgaaaatta tcatactcat
    15001 ctctatcaag aaaaatatca actattgaaa caaaacctat ttgactactt ttgggatgaa
    15061 gaacttggcg cttttatcga ttgctatgaa tctgggagaa gaaatgtgac gcgtcatgcg
    15121 aatattttcg ctgttttatt cgattttgct aacaaagacc aacagcaatc gatcttaaag
    15181 aatgtcttat tgaatgacac tatcacacag attactactc catatttcaa attctatgaa
    15241 caagatgctt tatgcaaact cggacagacg aatatcgtct accaaacgat tcttgattat
    15301 tggggaggga tgttatctca tgatgcggta actttctggg aagaatacga ccctactcaa
    15361 acaggaaatg aaaagtacag tatgtatgga gatccttatg gaaaaagctt atgccatgca
    15421 tggggagcaa gcccaatcta tcttttagga cgctatttta tcggtttgta tccaacaaag
    15481 ccaggttatg aagaatttat gatacaacct aatctagatt catttgaaac actgaattgt
    15541 acgctaccta tcaaagatgg aaagatttcg cttgaaaaaa caaaagataa gatcgtgatt
    15601 acttctgata gaacaggcgg aacagtgaaa atgaatggta aaagttatcc tcttctagca
    15661 aaacagccac ttgtcttaac aagctattca aaaataaatg cataaaacaa aaaactgctt
    15721 gcgacatatc agaagacttt aaatcttctt ctgatattgt ccaagcaatt tttattttga
    15781 agtttgctta attctgttct ttagaaacaa tgtttgttct ttttccggca aaaagaaagc
    15841 tagataatac gacaagtaaa aagaaagcga tgattccaac tgtcaaggct atttgaccgt
    15901 attctcctag tttcccaagt acaagtcctg gtactagata atcagtatcg gcaaatgtag
    15961 aagaagcgat tttaagatct cctaatactg gaaggataaa taatggcagc caactaatca
    16021 ataagctatt gaaagcagaa cctaataccg cccctcttct accgccagtc gagttgccga
    16081 agacaccaga agcagctccg cagaagaaat gtcctactac cccaggaatg ataactgtag
    16141 tttgcaagcc tatcatagca aacatggata gtgttcctac aaagaagcta acaaaaaagc
    16201 caattaatac agcattaggt gcatatggaa aaacgatagg aacatctaat gctggtttag
    16261 aatcgggtac gagtttttta gcaatccctt ggaatgctgg aacgatttct gccaacacca
    16321 ttcgaacccc ttgaaggacg ataacaaacc cagctgtgaa tgttccagct tggatcagtg
    16381 caaagattac agcgttgtct ccattgctta gttctctttc aacaaatcca ggaccagcta
    16441 acaaagcaag tgcgacatat acgatcgaca tcaagagcat gatcgataca gtagaatctc
    16501 gcaaaaagct gaaactttta ggaatcttta tgtcctctgt agaacggtct ttgtttccaa
    16561 aaaactttcc gatccatcca ctagaagcat aaccgatatt tccggtatgc cctaacgcaa
    16621 cttgattgtt tccagtgatg ttcttcataa acggttgaca aagagcaggc gtaatagtta
    16681 atagtgttcc ttcgaagacc ccgcctaata agatcatcca caaatttgca gtaaaccccg
    16741 cgcttactaa aatgactgct gttagacaag aaacataaag cattgcttga cctgtaagaa
    16801 aaatgaattt atatcttgtg aatctagcaa gtaaaatatt cacgatcatc cctactagca
    16861 tgatcaaagc agtaggtgta ccgtattgtt gcaaagcaat cgccactact gcttcattgc
    16921 taggaactac tccttgagta tgcagagctg tttggaacat cgaagcaaaa ggggttagtg
    16981 aattactgat taaaccagca ccgccgatca gtactaggaa tccgacaaat gttttgatag
    17041 ttccttggat cacttctgaa aatttcttct tctgaagaac gagaccaagc agtgcaatca
    17101 aagaaacgag gatggagggc tgactgacga ttgacacaaa caaattcaac aatttatcca
    17161 ttgatagttc ctcccttgaa tcatttttat tcttttgaca agatcttcgt tactttttct
    17221 tttacttctt cgctgtcaat gatcgattgc aaaggtacga gcttttcatc aggtatagaa
    17281 gttagcgtat ctttcaaaga atgctcaatg aagaaataat cagccatatc gctagataca
    17341 cttccaacat ccgtatgact tacttcaaac tcgtccacat ctgctccaag atctgcaagt
    17401 gcttgtttga tgttcatctc taccataaaa cttgttccta atccactttg acatactgca
    17461 aaaattttca tatcattcgt ctccttttct tatgatcgct aatatttctt cttttgttga
    17521 tgcgtgtaat aaatcttgaa gattttgttt attagaaagt agctttgtta aatttgccaa
    17581 agcccgaaga tgtgcctcat tatcgatagc agctaaacag atgaacaaac taatgggatg
    17641 ggctggatta tcaagcagat ccacaggttg ttgaagtttc agcaaagaca tccctacttc
    17701 gtttacgcca tcttctggac gagcatgagg taatgcgatg tgatcgccaa tatggataaa
    17761 cgcaccatac tgctccacgc gctcgatcat agctttgata taactttctt ctattttatt
    17821 ttctgataat agcggctgtg cggcaaatgt gattgcttct tgccagtttt ttggttgatc
    17881 cgataattgg atcatttctt ctgttaataa ttcagatagc ataggacgtt gatcctcctt
    17941 cttttctaaa agtttgttca ttttacgatt taatacttga tagagctttt cttcgtctac
    18001 gccttctttt aattcgatat aaggcagtag cgccgacaac aattcttggg cagaaggcat
    18061 tgaaaatcct ggtatcagcc aatctctttg tacttgattg atcagctgat ttttttccaa
    18121 gtcagtcatg attggatgca gaacatagag agactttttc ttatcagtaa gtgtgagagg
    18181 tacagtagaa aaaataatat cgtaagaagt ttcgtcaacc tcgtgtaagc ggtcagctga
    18241 attagtctct gtaaaaaaga tcatcgggaa taattgctga agttcacttt ttaagatcaa
    18301 tgaagagcta atgccacttg gacagacgat cacggctctt atttttctat ttctttcttc
    18361 tgcgtccgct tttctgatct ctccgccaaa tagtatagtg aaaaaaccaa tctcttcttc
    18421 agggatgctc ttccctattc gtctttctaa cggaagtaat gcttttcttg tcatttcgta
    18481 gatggaagca tattgatgct taatttgatc gatcatcaca ttaggtaaat aaaaagaata
    18541 ggaaatacga aaataggcag ggaccaagtg ataaaagaca ttcatcaata attcacgaaa
    18601 cgaagcaaat tctacggcca tcaggaattc catgcgatgg atgacttctg aagaacattc
    18661 cagcaaaaaa tcaagtgcag gctcctggac atctccttca acgatcgatg ctaagcaaag
    18721 agagaaaaat ataatctccg aattttgatg ttcggaatag ccaaggccag tcaatacttg
    18781 tcgactaatg tcagtgactg caagagaagc caacagttca gcatctttta ccagttccat
    18841 ggatgttttt ccaattcgaa tggttgtcaa actcaaataa taagctaatt catccattcg
    18901 atttggtgcg aatcgaagat ctgatagtga aatcgtttta tctagcaaat ctctaacttt
    18961 ggcgtaataa tcaatggaaa gggttgcggt taaatacgtg agcccaaatc ttccagcaag
    19021 tgactgcatc aaacgagcag tgcgattttt tgcttctctt cttaatattg cttctgaccc
    19081 tagcagccta aaaccagttt tcctagtata cgacagctga caatcttcca cttctaattc
    19141 atttcttatc tttttgatat ctgacaggac tgtcccttta gaaacctgaa gaaaatcttg
    19201 aaaatgaaaa acagaaacag acgttgcagt cgaataaacc atcaaataaa gaagatctct
    19261 tcgctcttgt tcggtataca cagcttcaaa tcgagtaata tccaaaaatt tttctgccca
    19321 gtcgattgct cggaatttct ttatacggat acgggtatca tccacatatt cggcaaaagg
    19381 agttaattgt ttgatcgcct cgcttgtatt tacttcgatg atctctgctg ctttttctag
    19441 actgatttct cttggtgttt tcaggagata actaattttg tagttcacag cgctgttcac
    19501 ctcctactta tattctagta tggtagcgct tttaaatcga ggggtgtttg gtacaactta
    19561 cagttggatt ttttttacga ctgtaccgaa cgatttctat cgaatggata cagaaaaaaa
    19621 gcaggtgacc aaatgtctcc tgcttttatg aattgatttc gtccgatcaa tgatgatcat
    19681 gtttattttt tgaggtcagt tcttcaaatt tttctttgac atcttcaacc gctttttcga
    19741 tatctttttc aaagtgattg ggctcattag gctttgtttc ctcttctaca gaatttgaac
    19801 cagataaagc tgtttcttta ttttctgttt caccagagtt tgtgccagct aatgattctt
    19861 ctttgtcttc tgtttcacca gaattagttc ctgctaaagc cacttcttct tctgttttag
    19921 gggccattga acctcttaat cgtttgtgtt tttcaccgtc ataatttgtc atgttggttc
    19981 ctcctagtag ttctatactt tttcatcgta caataagaca gtttatgcgt caaaaaacaa
    20041 acccgattct tacaaatatg tcatattttt ccttctgatt cttgaatgct tttgattcct
    20101 tcttccaaaa ttttcatttg taagattgtt tcttcatctt tgatgacttt ttctgcgcca
    20161 ttaactaatg tctcgtaaat cccttcgtag acacggctgt aatcaccaac ctcggaaatt
    20221 actttttctt catgataact cccttgttca tctatatagg tcaatacacc ataatgttcg
    20281 ggcaaatcga taccaaagtc ttcatgatca ggcatgtaaa acattttcag atgttcttct
    20341 tgtcgatctt ttgtttgttt cacgaacatc cctttttttc cgtaaacgac gaaacttgga
    20401 cgttctttga tgcggaaata actggatttt atcgaaattt tcatatttcc atagaaaaaa
    20461 tccagatcga agtaatcatt cattcgtcct ttccctaaaa gttgacgtac atcatagctg
    20521 atatcatctg gttcgccgaa ataagaaaga acttgatcta ctgtatgaca agcatgtcca
    20581 tataaatagc tgttcgcagt agaaaattca tgcgtatttt ctggtacttc cggtctgaaa
    20641 taatcataat gcatttctac ttctaaaata tcccctaatt ttccgctttc gattactttt
    20701 tggactgtca agaagtcaga atcgaaccgt ctgttttggt aacattggat aaatagacct
    20761 ttttcttttg ctaatgcaaa taactcttct gcttcctttg acgtttctgc aaaaggtttc
    20821 tctactagta cattcttacc atgttcgagt gccatttttc catattcata atgaaattga
    20881 ctaggtgtag tgatgacaac taattggatc tcaggatcat cccaaagatc agacagctga
    20941 tcagtatatt cgatgtcttc gaatgttttc cacactggtt cagatgggga aaaaattttt
    21001 ttgatattga ttttgttcgt aagttttctt gaaaaaggca aatgatagcg atttgtactc
    21061 tttccattgc caagataagc gattgtaagc atgagtggtg cctcctttta ttgatgtttt
    21121 cagtatatcc tagaaaagcg cagaaagtat ttataatagc tattctggta aaaaagttca
    21181 gaaaaacact tgttatcatt cggtttggtg aaattaatgt tcaaagtaag gataaaaatc
    21241 aaaaagatag tagcggaacc aagtgtttat cagtaagata gaatttggag gtcagatcat
    21301 gcttattgaa gaaaaactaa caaaacaaga gttgtttacc acgacagaga aacggattgt
    21361 cgattacatc cgtaggaata tcgaagctgc agtctacatg acgattgaag aattagcaaa
    21421 agctacgtat acttcccatt cagctatcat tcgtttatgc aaaaaaatgg gattcagcgg
    21481 atttaaagaa ttccgtttcg agctggggcg ggaagttcat caattgattg ctcagttcaa
    21541 tcaaacgatt gatcctaatt ttcctttttc aagtgaagat agcactcagc aaattgcaaa
    21601 gaaaatggcg gatttatcga tccagtcaat aaaaaaagca caattgcaga ttgaacatga
    21661 agatttagat caaatcggta aagtattgaa caaggcacag cgaatctttc tttttgcaaa
    21721 aggtgattct caaatcacgg caaggaaatt ccagaataaa atggtcaagt tgaataagtt
    21781 tttgatcatg gctgaagaat acagtgattc ttcttggaat gcagccaatt tgactagcga
    21841 ggattgcgcc atttttatta gctacagcgg tcgcatccat cattatgaac gaataatgac
    21901 ctatttgaag catgtagagg cccctacctt gctgattacc ggaaatcagc attcagaaat
    21961 ggccaaacag gctgatatgt gtcttgttat atcgcaggag gaatatgatt ttgctaaagt
    22021 agcgactttc tcctctcaaa ttgccttcga ttacgtatta aatacgttat tctcagtgat
    22081 atattctcaa aattttgaag agaatatccg caacttgaaa aacaaacaag aattgatgca
    22141 aaaaggcctt cttaaagaat atcggaaaac ttaagaaaag atcctcattt agtaataaga
    22201 aagatagtgt ttgataggct ctctttgata tcatcataaa aacaggctga agttttcttt
    22261 ttcaaacctc agcctgtttg tttacggaac gattcgaata gatatattat agaaatgagg
    22321 aatagctaac ttctgtttct tattttgaac gattgacgaa aaagatatat aagaaggagg
    22381 aaaatcagta tacatgcagc taacaatcca tacaggaaaa atcgtttgtt ctcggatact
    22441 ttctgtactt ctttttgaac ttcttttgca tgataaggaa cccggtgccc tctgacaagt
    22501 aagcgatgac tgttgaccat ataaggtgta caagttagga gtgtcacgaa atcctgccct
    22561 ttttcgattc tcagatcttt tgtatcagtc ggttctaccg tcttgatctg atcgacttga
    22621 taagcgagtg tccgtccatt gatttcgata tagaaagaat cacctaaagt cagtttcggt
    22681 aagtctgtga acaactttgc ttgaggcagt cctcgatggc tggaaataac ggcatgtgta
    22741 ttttctcccc ccgtaggata agacgttcct tccaataatg aggcaccttt ttctagcagc
    22801 acagtattcg tttcatcaaa aattggcaaa tggacattga tctcagggat cgtcaaaata
    22861 ccgatcgtat gtttctcaaa ataggttcta tcctttttcg caggttcttt tttcttagta
    22921 aagggatctg cacctggatt tccaccttct tttgctaatt gctggttttt ctttgtcatg
    22981 ttttcttgga ttttagccat gacttcttca ttttctttga ccgcttgttg ctgataatga
    23041 gagatgatct gctgatccag ataattattc agcgcgtcgc taacaaatgg ataagctagt
    23101 atccctattc ctgagagaag caagaaaagc atcagcagat ctagaagttt ccttttcttt
    23161 ggtttcattt gtgttctcct tgtgatgact gtttgataat aaaagaaaaa ggagcatttg
    23221 cgtaatccgc tgctcctttt tccaattctt gcaaaaagac taaatctgac tgtgcttgcg
    23281 tctagcaaag tacagtccag caatcagtag aaggactact cctgcaccga tatacacata
    23341 gattccctta ccgcctgttg aaggaagtgt acctttgtgt ttatttggta ttttttcagg
    23401 agaaatcaac tgtcctgctg ttacataaga ttgttcatcg atagtaaatg ctacacggtt
    23461 tgttaatgga acatattttt ctggcgcttt cgtttcttcc agatagtacg tgccatattt
    23521 tagacctgtc acatcgatta aaccgttact tgtggttgta aaaaccgttg ctgattcttt
    23581 cgccgatacc cagctgacgg cttttgtgga tgggtcgatc gataaatatt tcgctgtgtc
    23641 actatcttga tcacgaacga cgaattctgc tccagcaagt gtttggtctg atgtaacgtc
    23701 accatctact ttaacaaatc gtttgccccc agtaacgaca tcgactgacg gtggtgtttg
    23761 atcatttgta tgcccgttat cgacatttgc ttggttagaa aatcctttgg ttggatctgc
    23821 tttttcgttc aaatgcatat agtaaacgaa tttcaatgta ccgccaggag ttaatgaagg
    23881 aatataattc ggatcaactg aaaccgtgaa tccgtctgtt tgctcagtga cagaataatt
    23941 tactgggtcg atttctttat ttccatcata taaggcataa gcatacgttc ctgaagaatc
    24001 attatcaaat gttaaagcag cgtcatgagt atcgataagt ttgaatgttg tgtacttgtt
    24061 ttgcgttcct tctttatcag cgatacctaa tgggatattg acagaaatct catattgaat
    24121 tttttcaccg attgcgacat cttttccatt caattgaggt gttgttttat caactttaga
    24181 cgtatcattt ttgatggtct tttctactgg tgtttggcta tttgccagga tctcaaaagg
    24241 cgtgattgtt tgcttttcaa tcatttccgc attatcagga gcttttactt cttctaaatt
    24301 atattttcca acttctaaat gattaacgat caactggcct ttttcaatag atgcctcggc
    24361 aaagtcattg ttgccgatgt cataagaatg accagtaatg aaatgtttgg ctttggtttg
    24421 atcagttgtc caagtgtaca atccatctgt gacactttgg atgtattttt tgacgcttgg
    24481 tgttccttct tctttagaaa taataaattc tgctccattt agtgcttcgt tttcggcagt
    24541 accgattttt gtaactttca acgttccatc attaccgact gtatttttag ggtagagatg
    24601 gatagtatct agttcttctg tcccgtattt ataagagcca tctgcttgtt tgatcatctc
    24661 atatacaggg aaagctaaaa ccatgtttgc ggcagctgac actccgtctt ttggttcttc
    24721 tttgatcgtg tagactgcat ctttcccatt ttgtttttta ggtaaagata aagtgacatt
    24781 tccatcagca tctgtcgttc ctgaagcaac tggtgtacca ggagtcaaag actggactgc
    24841 ttgttttgct gcatccacgg acgctccttt atctcgttgc gcataaaatt cttgagtgac
    24901 gttataaact gaaaatgaaa tatcggctaa tccttggtat tgatcgaatt cgctcatttc
    24961 tttcccgctg ttttggatta aaggatcggg taaatcagtc attttctttt tatgaaggat
    25021 gacttgagct gctgtctcct ctgcaaacac tttttttgcg ccgaagctgt ttgtgagtaa
    25081 tggtaaaata aggaaaagga ctcctaacat aacgtttatt tttttatgat ttttcatttt
    25141 ttcactcctg ttcttttttt gataaacaac cagattgcaa agcttgcgaa taccatacca
    25201 acaacgccta aaagtgtatt tttcgtttcg tttgtttttg gaagaaatgg acggctattt
    25261 tcttttttat tgactacttt caatatactg tccgcctctt tatcctcaga aagaaaagtt
    25321 gaaatggtaa acggaatttc tgtttcactt aaaatataac cagaaggtgc ttcgatctct
    25381 tttaatcgat aacttcctgt tttcaacccg gaaattgaaa aagcgccatt tttatcggaa
    25441 atcaggtgaa gcgcatctga ttcgttcttt gtccacttgt atccgttggc tgtttgctgg
    25501 aggtaatttc ctgctttgtt tttaataagg aatactgcgc cagctaatcg tttatctggt
    25561 ttatttgtct cgattttgac aaattgctta ctccctgtat atacagagac tttctttgta
    25621 ctgacaccaa aaccaaaatc cagtgttccg ttgttgttga tttccttgtt cgcctgtgct
    25681 gtactgctta gctgcatctg ataagaaatg gtcagttttt ttccagcata tttttccaac
    25741 gaagggatcg aaaataaaac atcaaatccg tgaggtttct tgtgtatgac aaagccttct
    25801 gtcagctttt ctccattcga tgaaatcgtt aaactattag gtaaaaacgt taatgcttca
    25861 tccgcactat ctgacaattc gaacttttga tagtcaagga tatttacagg aattgtcgta
    25921 tgcaaggaat aagtgatttt ttcaccaatc gtaaaatcat ttctaggctc ttctaatacc
    25981 ttctcaaaag gtggatcagg gtagtcgttt tcttcatttt taggatacaa atggatagtt
    26041 gaaagttttt gattgttttg tccatgtaca ggaagaacaa ctaccatatt ctctgccttt
    26101 tctttgacga cttctggtac tttggattca atgaaaagat acgctttatc tcttttttct
    26161 tgatctttag cggccagaga aaaagaagcg ataccatctt cgttgttaag agttgtagtt
    26221 gttgcttctg cggtaaacat accggaagac gcaccgtttt ttgcgatctc tgcttgtgct
    26281 tcttctaccg ttttgccctt ttcccgtaga tggtaaaaag aatctgtgac atcataaact
    26341 tggaatgtga caccatttaa tcctcgatac gtttgtaata aagctttttc ttgtccgtca
    26401 tttggatgat ttttcggcag ttgcccgttt gggaaaagca atttgtgtaa agtaatctga
    26461 accatttctg tttctgtttc tgttgctacc tgagtaaaag ctggtttaaa tagtaacaag
    26521 aagagacaca tactaagcca accaagtttt ttcatattta gctgccccct ctttcttttt
    26581 tcaaaaaaag ataccaaata gaaaacgcac aaccaatcat ccctactaga tagattccta
    26641 aacggccaat tcctcccgtt tcaggtaatg gtacttttgc ctgattcgtg atgtctaaag
    26701 aaatctggtt gtttttggct cctggtgaca gaacagattc atgatcttgt ccatccactt
    26761 gaattgaccc atcttcgtgt ataactatag taactggctc ttttagacct tggtatcctt
    26821 ctggtgtaaa agtttcggtc agcgtataag ttccaggttt taaattttcg aatagaaagg
    26881 tatctgtttc ttgtccatct tttggcgatt ctaagtccat ttctggtccc tgcagtctga
    26941 attttgctcc ttttaatgtc tgtccgttgt cttctttttt gtgtaccgtt agatcaaacg
    27001 cttttagtcg attttgatga gtgagcgtcc atacattatt tgtcatctcg gtattttttc
    27061 ccgtgtaaat gatttttcca tatttatcga tttggaattc ataatttccc ggcgaagtat
    27121 catatccggc aggaccagct gtttcaacga ctcgatattt accaggtgtg agtgaatcaa
    27181 aactggcaag acctttttca ttcgttgttt ggctgtcaat tggctgataa gtaccatttg
    27241 cttctttttt ctgtagggaa aatgctgctc ctttaagatt tatctctttg tccgtccctt
    27301 gcatcgcata cttacgtact gctacaggaa cttcaacaaa aggattttca atagtcaact
    27361 ggatcgtatc atcggaagta gtgactgttt ttccatcaat ggttaccgta ccatcagaag
    27421 cgatcttgat ttcccaagta gtctttttgc ttaatccatg cccttctgga gcttttgttt
    27481 ctgtcagcgt ataggtcatt tctttttgca atttgacatt ttctggaaga gaataggttc
    27541 cgtcgccatg atctgttagt aaagtatcaa tagaatctcc tgttaatttg aaaacagcgc
    27601 caataagatc cttttcacct gtagaggaat ttttcgttat tttcaagttt aacggtgtga
    27661 attgtttagt atttttccat gtcattgcat ctatttgttg agaatcgtat cctggtacag
    27721 gtaattcttt gattgtttgg taactgaatg cttgaccttg attattgtat tgaggtagtg
    27781 ataatatctc ttgataactt tctccgctat ttgcagataa tttgtcaatg tctgcacgtt
    27841 cccacgtatt tgttgtatct ttagctggtt taatgattcg aatatatccg tttttccaag
    27901 ctgcagcatt tgtcgtatgt tcccgttgaa tctcaaaagt aacttgatct ggacgatcag
    27961 ctagattgtt gtcaaattct tcccataact ttttgatgtg aagactgact ccgggagctt
    28021 tagcagatgg tataccgaat tcagctaatt cattggtatc aatgtttggc tggaaagttg
    28081 tcctgccgtt catttgatac cagaaatttg gatggaagtc ctcattttct gtttggattc
    28141 tcacttggta atggatttgg atttcttggt cttttcctaa atagatctga ttgctcttga
    28201 tggtatttcc ttctatggaa atagatggag tgaccgttgt aggacttgtc cccacactct
    28261 tgactgaaag tgtaccaggc tgataaatga atggttctgc gattggatca tttatttgtc
    28321 cattgcttac agttgctgag atctgtacag cttttttggc taggtattca gagatatctg
    28381 ttgcatgatc agctgattca tagtaaagat cgcctttttc atctgatgaa accatttgtc
    28441 gcatacgaga ctctacttct gcttttgaga gaccagctgc cggatcgctt tgaagttgga
    28501 tgccaagacc atgtatttcg attcctcgtt ctttgagtgc catcgcttct ccgatggttg
    28561 cgataaacgt actgtcgatt cttctggata gattgttttg gtcaggtgca tcatagattc
    28621 ttgagattag agaagtattt cccggccgat cttgcgtatt agaaaactga gttccgtaat
    28681 aattgctgct tgattgtgcg tgtacccgct gtactttata ggaaaatgtt ggtacaccat
    28741 ccgtcagcaa aacgatcact tttttatgtc catttggaac ggatagcatg cttcctgcat
    28801 ctcttagtgc tttttgtgta aaagtaccac catttgtccg tgaaggtgta atggatttta
    28861 cttgattttt cactgaatca aatgaaccca tctgtactgc accgttactg tagctatatc
    28921 cttcgcttga gtagccgaca tatcccatat tgattttgtc tgtgataccg ctatctgcta
    28981 aagtatcgac aaaacgatcg acaccaatct ttacttcacc gatccgatta ttgtcgttca
    29041 tacttcctga ccagtctacg accaatacga gatcaagagg cgtgatatct ttttgtacat
    29101 ttccacgagc attcaaataa acatcaaaca atccgggtgt acttgtttcc tttgcatact
    29161 ttcgtatcgc atagtctgct tgattaccgg ttcctccata ttcgatatac gaatgggtcc
    29221 gatcattagg atctccattc caagaattta taccatccca ttgatttgtt ccgtttttat
    29281 tgccttgatg atcaagaaca tttgtatttc ctgtaggttg ccaactggct ttaggataag
    29341 tgccctgatc atctgttgta tattctgtag gaatcgtttg attagatggc acactcacag
    29401 ctggcaagga taatgtaggc aatgtaaatg ggctgacaga attttcgttt tcttttgatg
    29461 attcttcttc tatttcgtct ggctttggta ctgacacatc tgtatctttc ttattttctg
    29521 tctcatcaat aatgtcatat tctttattat cgacgatcag cttgtttgcc tggctgtggt
    29581 aaagtgctgg atcgattgga aaactcagat cgatagtttg tttagtgttt gatgagattt
    29641 ccaacgtaat tttcccgcta tttacctgat aagcatagcc atctccttct tttctttcca
    29701 catcacttaa gtaattttcg actagaccaa tttctttttc tatcttttca gcactggtgt
    29761 tattcgcatt tagcttgatg ttcagtgctt tttttccaga ctgatccgaa atgacttcag
    29821 ctgaaattgt ttctaaaact gttggatcat ctgtagtgtc tgctattgca gataatggca
    29881 caaagtttga taaactcaat attatcagca tgaaaataac tttcagtttc ttccctgtgg
    29941 tcgtcatttt tcccctcctt tttctttttt tctgttttga cgtgaatagt gaaaattatt
    30001 aaaataaaca ggcgttacat aattttattt attttaatat gataattatt atacgaaggg
    30061 cgctaaaggg aatcacacca attttcatac caattggttt catttatgaa gttgattcct
    30121 tcgattaatg agcgtttcat tgaaagaaaa gggttgaggg agaaaaacat atgatcaggt
    30181 tgttggaacg aaactataga aagatggaaa aaatcattcg aattctgtcc atttctgaca
    30241 agtggatgac taaaaaagaa cttgccaaac gaattggtag ttcagaatct acatttgtaa
    30301 ggtatatcga agagatcaaa atccgctggg gaacggatgt aacgattcaa tcttcacaaa
    30361 aattggggta tcgtcttgaa catttgaatg taccagctta tttaaatgtt ctgatggaaa
    30421 tgtcgcaaag ttctacaaac agtcgtttat taaatgagat tattgtcaat ccgggtaaaa
    30481 cgattgagta ttattgtgaa aaaatttcca tcagccggtc ttcttttaca cgtaagttga
    30541 aacaatgcaa tcaaatttta gaaagctatt tattaaaagt gatcgttgac caaggttttc
    30601 agttaactag ccttaaaagt gagctccagt tgcgtatctt tgttaccttt ttctttttaa
    30661 gttactacgg aagacgcacg ttgccatttc caatcgaaaa aaagaagatc aaagaaatta
    30721 tggtcaagaa taattgtcag atgaatttgt ttcaggaaga aagcccatat gagcaatctt
    30781 tctttattat gtattttgtt gtgcatctca tgagagaatc ccaaggaact ccatttgaat
    30841 ggaaagctgg agactcttat gtacagttga taaatatcga taaaaaagac ttcttaatct
    30901 tgaaaaatct cttaggaaaa ttgaagtaca gcagctatat aaaagtagtg aactattttg
    30961 tcaatacgat tttttcagaa ttttcggaga aagaaaaaaa tgaaatcagt gagcagatac
    31021 gaaaaaatat gtcctactgt tcgttttcat ccgctgattg caaagaaaaa aacgaagaat
    31081 atgttgttca attattaacg caaatttatt ttttctcccg tattttccca tacgatacga
    31141 ctcatttgac gatccgtgca acttactttg cacagcagat gcaacaccgc cagatacgtc
    31201 tatatgctgg atttcgtgca gaacttcaat tttattccac tctactgaat cagaatctga
    31261 tttattattt atctagctat atcttttgga tgcttatcgg taatcctgaa attcaccatt
    31321 atacttctga taaaaaaata ctgattatca gcgacttaag tcttcgacat agtcaatata
    31381 ttgaagaata tatcagtgat attcttgctg tgcataaaat tcactctgag acaactgcta
    31441 ttactgaaga tcagctgtcg aaatacaatc ttctcgagta tgatctgatc gttacgaatc
    31501 aacccatttt gaatgcccat gttcctcata tattgattga tgatagtgtg tcattcgcaa
    31561 atgaagaaga actcatccgc ttattcgagt tatgaataat caaaaaacag ctatgtataa
    31621 accaccctga taaaaatttc aggacaaata cacatagctg tttcttttta ttgtattcct
    31681 tcagcaatcc aacgagattt atagcgaaag tcttttaaca actcttcttt tgtccaatta
    31741 tttgcaatga gatccgtcaa agtgttcatt acgagggtca tggaaaagta taggaaacta
    31801 actgcttctt ccgacgattg aatggagaaa tgattgattc ttaataattc cagccatttt
    31861 tcgttcattg gagaatcagc cgatagttct ctacgtttgg ttgtctgctg atcatttcgc
    31921 tgggttaaca agtataacct ttgccaatag atactttcgt gatctaagtc gcagcaccac
    31981 tctaaatatt tttcgatccc caaaaagaaa tgttgtttgt tttcgctaat gaattttaag
    32041 atatcttgat ggactatgtg agaactgctt ttgatcgtat acaaataagc atcttccaaa
    32101 tcttgaaaat atttataaaa agcaccgcgc gacatcccca tcttgtcaac gatttctgag
    32161 actttgactt ggctgatgtg ctgtgagcaa aatatctcca gtaataggtc atcgattctc
    32221 tgtttctttg cgttcgataa attgaaaaaa gtttcttttg gcatccacat ctctccttta
    32281 tctctgatta tacgtggata gcccttcatt gtcatgaagg attcattcct tctttttctc
    32341 ttcttctgtc aagtacccat cttccattcg ataaacgcga tcactccatt gaatcatacg
    32401 gacatcatga gtaaccataa tagtcgcttt ttgtttctca tgagcttctt taactagtaa
    32461 tttgacgact tcatatgcat gatctgtatc caaactagct gttggttcgt ctgctaagat
    32521 caagcttggc tcattatata aagcgcgagc aattgccact ctctgtcttt ctccaccaga
    32581 aaggtcttta ggataactat ttttaagtga ctggatatct aaagattgca gcaaggaatc
    32641 gatttttgtt gaatcttctt tttttgaagt aacttggtca actagttcaa attgttcctt
    32701 taccgttaaa aaaggaatca aattagagct ttggagaata aatcctattt ctttaaagcg
    32761 taaatctgct cgtttttttt ctggaagctc agttagtgag tgaccgttga tcactatttc
    32821 tccggaagtc ggtgtttgca atcctcctga aatggtaagg aaagtacttt tacctgaacc
    32881 agaaggacca atgatgctga cgaattctcc ttcctttaca taaaaatcaa ttgctttcaa
    32941 agctgagacc tttgtatgac cagaaccaaa tgttttggta acttttttca tcgttaatat
    33001 ttttttcatt tttattaacc tcctattgct gtaattgggt ctactttggt tatcgtacgt
    33061 atcgaaaaca atccgcctag cattgcgaca atgatcaaaa tcccactata tagcaaccat
    33121 tgtgaccaaa aaacagcaaa aggcatcgcg cttggtaaaa tcagactagt caaataagca
    33181 aacaaaagag caagcagaac gcctaccacc ccaacaataa aggactgtgc tactaaagat
    33241 ttagcgatga agaaattttt gatcccttgt gctttcatta ctccaaaaat agcagtcttc
    33301 tgaagtgtca tcacgtacat gaaaataccg accacggcag caaccactaa gaacaaaaag
    33361 tatatcatcg catttaaggt catattttgg gcagaatagc caggaatact ttcaatgaat
    33421 gtcccaatag ttaataactg tagacccttc gatgcttttt ctttcgaaat tttagctgaa
    33481 tcctttatta cgatcccatt gatcggctgt tcttcttcag aagcaaacgg ctgttgtcca
    33541 tacttcaaag cagtccacgt atctagggac gcatagatga cgggagaaac tgtgtaataa
    33601 gtttcaggaa agataccgac aatcgtcaaa ggatcgtcgt aactgccaat tttgatcgta
    33661 tctccaattt tatagcctga atcagccaga ttttgagaga tgatgatttc attctttgtt
    33721 ttaaattcat gccctttcgt gagttttggt aataaaaatg cttctttcgt tgtgccgaaa
    33781 actgtaacat tttctttgtt ttttcctttg attgctccgc tgtacaatcc gattggtgct
    33841 ttctctcctc cttcaatgta tttcagatct cctcttgtta attgtgaagc tgcaaatact
    33901 tgatttgcat cctccgacaa aacaatctct tgtgcgtccc aatcatcgat tgctttttta
    33961 aactcttctg aaagtccatt cgctagtcct gacaacatga atacgacata cgcaatcaag
    34021 aacataatac cgacgatcaa gccatatcgt aatttagaat agcgaatttc tttgatagct
    34081 agaaacattt gtttttcctc cctatgatag attgatagtg acaaaatgtc acttgttgaa
    34141 tagtgtaact ctaaagtgac aatttgtcac ttgtgttgtt tttttcttat caataaattg
    34201 tctaagacat ctttcgataa aaccttaaca tgagtcataa aaaatttatt taagccttat
    34261 ttatgctttc catgataaac taaa
//
LOCUS       DACEQH010000026        34097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.026, whole genome shotgun sequence.
ACCESSION   DACEQH010000026 DACEQH010000000
VERSION     DACEQH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.026"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            256..2421
                     /gene="acm"
                     /locus_tag="IW833_001333"
     CDS             256..2421
                     /gene="acm"
                     /locus_tag="IW833_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ1307462.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2693..3178)
                     /locus_tag="IW833_001334"
     CDS             complement(2693..3178)
                     /locus_tag="IW833_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ1307463.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3658..4299
                     /locus_tag="IW833_001335"
     CDS             3658..4299
                     /locus_tag="IW833_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1307464.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4318..4953
                     /locus_tag="IW833_001336"
     CDS             4318..4953
                     /locus_tag="IW833_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1307465.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4971..5786
                     /locus_tag="IW833_001337"
     CDS             4971..5786
                     /locus_tag="IW833_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1307466.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6105..7541
                     /locus_tag="IW833_001338"
     CDS             6105..7541
                     /locus_tag="IW833_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1307467.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7730..10177
                     /locus_tag="IW833_001339"
     CDS             7730..10177
                     /locus_tag="IW833_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1307468.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10407..11474
                     /gene="rlmN"
                     /locus_tag="IW833_001340"
     CDS             10407..11474
                     /gene="rlmN"
                     /locus_tag="IW833_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1307469.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11667..12770)
                     /locus_tag="IW833_001341"
     CDS             complement(11667..12770)
                     /locus_tag="IW833_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1307470.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13323..13871
                     /gene="aac(6')"
                     /locus_tag="IW833_001342"
     CDS             13323..13871
                     /gene="aac(6')"
                     /locus_tag="IW833_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1307471.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            14417..16078
                     /locus_tag="IW833_001343"
     CDS             14417..16078
                     /locus_tag="IW833_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1307472.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(16148..16495)
                     /locus_tag="IW833_001344"
     CDS             complement(16148..16495)
                     /locus_tag="IW833_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ1307473.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16579..17520
                     /locus_tag="IW833_001345"
     CDS             16579..17520
                     /locus_tag="IW833_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1307474.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17521..18564
                     /locus_tag="IW833_001346"
     CDS             17521..18564
                     /locus_tag="IW833_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1307475.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18570..19640
                     /locus_tag="IW833_001347"
     CDS             18570..19640
                     /locus_tag="IW833_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1307476.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19630..20583
                     /locus_tag="IW833_001348"
     CDS             19630..20583
                     /locus_tag="IW833_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1307477.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20595..21605
                     /locus_tag="IW833_001349"
     CDS             20595..21605
                     /locus_tag="IW833_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1307478.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVKKSQVVEKMGRHSVI
                     KG"
     gene            complement(21890..22711)
                     /locus_tag="IW833_001350"
     CDS             complement(21890..22711)
                     /locus_tag="IW833_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1307479.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22808..23086)
                     /locus_tag="IW833_001351"
     CDS             complement(22808..23086)
                     /locus_tag="IW833_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307480.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23354..25363
                     /gene="metG"
                     /locus_tag="IW833_001352"
     CDS             23354..25363
                     /gene="metG"
                     /locus_tag="IW833_001352"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ1307481.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(25842..26429)
                     /locus_tag="IW833_001353"
     CDS             complement(25842..26429)
                     /locus_tag="IW833_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307482.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            26604..27926
                     /locus_tag="IW833_001354"
     CDS             26604..27926
                     /locus_tag="IW833_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1307483.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            28081..28857
                     /locus_tag="IW833_001355"
     CDS             28081..28857
                     /locus_tag="IW833_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1307484.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            28850..29419
                     /gene="rnmV"
                     /locus_tag="IW833_001356"
     CDS             28850..29419
                     /gene="rnmV"
                     /locus_tag="IW833_001356"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ1307485.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            29412..30296
                     /gene="rsmA"
                     /locus_tag="IW833_001357"
     CDS             29412..30296
                     /gene="rsmA"
                     /locus_tag="IW833_001357"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ1307486.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            30747..31376
                     /locus_tag="IW833_001358"
     CDS             30747..31376
                     /locus_tag="IW833_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307487.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(31500..32609)
                     /gene="ugpC"
                     /locus_tag="IW833_001359"
     CDS             complement(31500..32609)
                     /gene="ugpC"
                     /locus_tag="IW833_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ1307488.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            32818..33240
                     /gene="mgsA"
                     /locus_tag="IW833_001360"
     CDS             32818..33240
                     /gene="mgsA"
                     /locus_tag="IW833_001360"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ1307489.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            33563..>34097
                     /locus_tag="IW833_001361"
     CDS             33563..>34097
                     /locus_tag="IW833_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1307490.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT        11357 a         5912 c         7056 g         9772 t
ORIGIN      
        1 aaattggaaa tatagattga aaaaatgaaa gcaataagta aaataaaatg aatatatgcg
       61 aaaaaataat ttacattagg aaaaaccttg taaaaacagt tttttggcat atttattttt
      121 tataaatatt tgtaaaataa ctatcaattt tttagttaaa agattggaaa tgagtggggg
      181 aggaattgaa gaaaggattt ttgagagatg atatagtagg ttttattttt agttaaaata
      241 ggggagatag aaaaaatgaa aaagtgtaga aatattattt tatcaatgtt atttattatt
      301 actaatataa catcactgat tcctgttcat gtctatgcgg atgcaggcag agatatcagc
      361 agtaatgtca cttcgttgac cgtggaccca acgaatatta cagatggagg aaacataaaa
      421 gtaaaattct cgtttgacga aaaaaaacaa aatatccaac caggtgatta tctttggatc
      481 aattggccaa gtgaaggaaa tattcgcggt gaaggattcc aaaaagaaat cccattaatg
      541 attgaaaata aaaatgttgg aactttaacg gtcagaaaag attctgcgca ggttgtgttt
      601 aatgaaaata ttaaaaacct agactcagta gaaggttggg ggcaatttga gatacaggcc
      661 agaaacgtaa ccgataccgg tgaagaaaat acgggaccat tcaccgtgac gagcggtgat
      721 aaaacagcta ctgtaaatgt gacaaagcca gcttctggtt cttcttctag cgttttttat
      781 tataaaacag gagacatgct acctgaagat actaagcaca ttcgatggtt tttgaatatt
      841 aacaataacg gcacttatgt tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga
      901 caaagattag atccaagtac gtttgaaatt aatcaaatac acttaggtga acagaaggtt
      961 tatagaggag aagagggaat ccaacaattt ttacaagatt tccccagcgc tacatttaat
     1021 ttttcagtaa ctgataatta cattgaaata actataccaa aaaactttgt caatcttagg
     1081 aaaataatgg tgagttataa gacgatcata gaaaacccag aacaaataaa ttttgagaat
     1141 cattcggaag cttggtttaa agaatttaat aaacctgctg ttgatggaga aagtttcaat
     1201 catacagtta aaaatatttc agcttctggt ggagtcaacg gtacagttag aggcgaattg
     1261 aagattttca aatatatcaa tgacacagag attggtatcc caaatgtgac tttcgaatta
     1321 agacgagcag atgaacagcc tattcaagga cagtctagta tcctgttgac ttcgaatgaa
     1381 cagggagaag ctagtatcaa aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa
     1441 gcacccaatt ggattgattt tgatccacta agttcaaatg aactcaaatt ctccataaac
     1501 gaaaatgata cagagggagt cagtttacct atttataaca agaaaaaggt tacgaacatc
     1561 acagcaacta aaatttggaa cggtggaacc actccaagac cgagtattta ttttaaatta
     1621 ttcagagcaa gtacaaacaa ttgggaacca gttcctgatg cagaaacaaa acgattagac
     1681 aatggaataa cttcagtgac ttgggaagat atccaacagt atgatgatag tggaaacgaa
     1741 tatacgttta aagtgcaaga agtagatcaa aacggaaatg attatgtccc ttcaggttat
     1801 caaaaaattg aaaacgggct agtcgttaca aatgagaata aagaagtcat ttcagtctca
     1861 ggacaaaaaa catgggaaga caatgagaac caagacggca aacgaccgac gacgatcaca
     1921 gtaaatctat tagccgacgg tcaaccgatc caacacaaag aagtaagtga aaaagatgat
     1981 tggcgctatc gtttcacaaa tctgccaaaa tacagagacg ggcaagaaat catctatact
     2041 gtaacggaag acaacgtgcc cgaatacagt acgacgattg acgggtataa catcaaaaac
     2101 agttatacac cggaaaaaac aaatatatct ataccaggta gccatataac accgtggaaa
     2161 aattttccag agaagacaga gagagagaaa attagaaatc tattctctca aaaattgcaa
     2221 atatttgctc atacaggaga atccagcgaa caaagaagca attatattag taagagagct
     2281 gtacctaaac agataacaaa taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa
     2341 tctagttttg ctgtaatcat tggtttattg atagtaacta tctgtgctgg gatattctta
     2401 caaaaatata aaaaagttta gttagaatat aatatttttt gttaaatatg actgaaatca
     2461 aaagaataaa attccgattt ataatcgaaa aaagaagcat ttttatatat gcttcttttt
     2521 tcgtagaatc aataaatatg gatttggcta actatggaag aatactcgta ttagttttca
     2581 gcgttcatac tagttgttac attgtttgat tggccaaaag aatttctgat atattttgtg
     2641 ctaaataaag aaaaagacgc ttgaattggg aaataagaaa actactctat cattacaaat
     2701 tttcataaac tggcagatgt tctaagtaaa caagtatctt ttcaaagtcg gaaaaatcca
     2761 taatcaacgt agtggtattg atatttggat gaaagcctac ggtatcttga cgatcaatag
     2821 cagcatcgat cacgacttgg atttttttct ctttgtcgtg catcaatgct aatggagtca
     2881 ctgtacctgc tggaacgcct aggaggtcag gaagctgttc ttcagaaaca aaagataagc
     2941 gcttttcttt taattgttca gctagttgtt ttaaatcggc gtgcttttca tcaggcaaga
     3001 tgacaagata gatttgtttt ccttttcttg atttcaataa tagatttttt acttgcggac
     3061 caggtaggga aaaagggaca tttttgactg acgtaatcgc aggatgatcg actttttgaa
     3121 aagagatacc cagttttttt agtagttcat aagcttcttg ttcggttgca cgactcatcg
     3181 cgcatcaccc tccttttttt attagtgtat cataaaaaag agttgacaaa tgaaaaataa
     3241 tcatgtaaat tacacgtaaa gttaaaacaa atgacatcga aaagaagagt agccgttagg
     3301 tgtttttaga gagtctctgg ttggtggaaa gaggcagcag ttaaccgtga aacacatctt
     3361 ggagtcgatt ttctgaagcg tatagtagga aaatccgggg agcccgttac agctgcggtc
     3421 gttgctagat gaccagataa ggctggcttt tccgtgagga gtcggtaaaa aaaggtggaa
     3481 ccacgtgatt aacgtccttt gatcttcgga tcaaaggact ttttgttttc atctgaacgg
     3541 caacgactgt gtgaggtttt ttctgcaagg aaaaaataaa cagaggtgga accacgtata
     3601 atacgtcctc ttggcatttg ccaagaggat ttttttattt taaaggagga aaagaagatg
     3661 gagtatttga ttgaagttat gccgcaatta ttagtaggag cactcacaac attgaaatta
     3721 tttttattta ccttgattgg ctcattacca ttagggattg ttttagcatt aggattgtct
     3781 ttgcatttta agccgctacg ctacttgctt caaacatatg tatgggttat gcggggaaca
     3841 ccgctgttgt tgcaactgat ttttattttt tatgggttgc cgacgattgg gatcgttttt
     3901 gatcgtttcg aagcagcact cattgccttt atcttaaact atgcagctta ttttgcagag
     3961 atctttcgtg gagggataca atcgattcct caaggtcaat atgaagcagc gcaagttttg
     4021 caattaagtc cctttcaaac ggtgacgaaa atcgtcatcc cacaagtgac aaagattgtt
     4081 ttaccttctg tagggaatga agttatcaac ttggtcaaag attcttcctt gatctatata
     4141 ttaggtttag gtgacgtaat gcgtgctggg aaaatcgcga tggaacgcga cgcgacttta
     4201 ctgcctttgc taggcgtggc aatcatctat cttacattga ctggtattct aacaatcata
     4261 atgaaacaag ttgaaggaaa attaaattat taccgctaac tgataggagg aagaaatatg
     4321 attgaattga agaaagtgaa caaagcattt ggtgaaaaaa aaataatcaa agatttgaat
     4381 ctaatcattc cagatgggaa aacattagct atcgtaggtc cttccggtgg cggtaaaacc
     4441 acgcttttac gtatcttggc tggcttagaa agttctgaca gcggagaatt ttatttagac
     4501 ggtgtaccgt tcaacccatt cgagcttaaa atgaaagaac aagttgtagg agtcgtattc
     4561 caagacttcc agctatttcc acatctgaat atttttgaaa acattacctt agcaccaaaa
     4621 atggtattga aacagacaaa agaagaatac accaaaaaag cagaagaatt agcggcttca
     4681 ttagggatta gtgatttatt gaatatctat ccttatcagc tttctggcgg acaaaaacag
     4741 cgggtagcga ttgccagagc attggctatg aatccacggg tattggctta tgatgaacca
     4801 acaagtgctt tagatcctga attacgccag caagtcgagc gtttgatcct atcattgaaa
     4861 gaagatgggg taacccaaat tattgtcacc catgatttag tatttgcaga aaatgtcggt
     4921 gatcaattac taaaagtttc cccaattcat taagaaagga gaagatagag atgaaaaaaa
     4981 ccagttttct attcgctttg atcgcaggtc ttcttctttt tgcaggctgt tcgaacaaaa
     5041 aaacttcagc agataattgg gcgactatca acgaagagaa aaaaatcgtt ataggtttag
     5101 acgatacatt tgttcctatg ggatttagag ataaagacgg gaagctcaca ggatttgata
     5161 ttgatttagc caaagcagtc tttgaacaat atgggattac ggtagacttt cagcccattg
     5221 actggtcaat gaaggaattt gaactgaaca atggaacgat cgatttgata tggaacggct
     5281 attccaaaac gccggcaaga gaaaagaaag tgcaatttac aaaaccttat atggaaaacg
     5341 atcaagtgct gatcacacct aaagaatcag gaatcacaaa ctttggacaa atgaataata
     5401 agagattagg tgcgcaaaat gggtcatctg gttatgatgt atttacgaaa caaccagaag
     5461 tattaaaaga gatagtcaaa aaccaagatg ctgttttgta tgatagtttt aatgaagcat
     5521 tgatcgatct gaaaagcaga cgggtcgatg gtctgctgat ggataaagta tacgcagact
     5581 attatttgaa acaaagaaac gaactagaag acttcaacat aacaaaaggt tcttatgaga
     5641 gtgaggattt tgcagttggt gcacgcaagt cagatagtac attagtgaaa aaaatcaatg
     5701 aagccctaga tcaactagag aaaacaggtg aattccaaaa aatatccgat aaatggtttg
     5761 gcgaagatgt tacgccgaga aattaacaaa taggggactg tgacaaaaga cgtgtcacag
     5821 tccctttttc cgaataaaca gtgttctcct gccaactcct tgtgactcgg agctgaatgg
     5881 caggctcaca acctcttggt aaacggtgtt caaaagccag tttttcggta ttaagccaaa
     5941 tttatgcaaa aatgtagaaa gccattttca caaaattttt cttactactc gaaactaagc
     6001 gactttttca caacctcctt tacataattt tattttgttg tggaaggggt ttcgtgttat
     6061 tctaaaagag gaacgattga aggttatagg aaaggaagag atgaatggaa cacaaagaac
     6121 ttacctcatt tccaaaagat tttctgtggg gctctgcttc tgcagcgtat caagtagaag
     6181 gagcttggca agaagatggg aaaggagaat cagtctggga tcgatttgta cggatacctg
     6241 gaaagacatt taaaggaacg aacggtgatt tggcagtaga ccactatcat cgttataaag
     6301 aagatattgc tttaatgaaa gaacaaggtc tgaaagctta ccgcttctct gtagcttgga
     6361 cacggatttt tcctaatgga cgaggagaag tcaatcaagc tgggctcgct ttttatgaga
     6421 gattgatcga cgaattgatt gaaaacgata ttgaacctat gttgacttta tatcattggg
     6481 atcttccaca agcattgcaa gatgaatata atggttggga atcacgtcag atcattgaag
     6541 atttcactca ttatgcagaa acattatttg aggcattccg cgggaaagtc cattactgga
     6601 ttagtttgaa tgaacaaaat atcttcactt cattaggtta tttgttagca gcacatccgc
     6661 caggtgtgac agacccgaaa cgaatgtatg aagttaacca tattgctaat ttagcaaatg
     6721 catctgtcat caataaattt cacgagttag aaatgcctgg aaaaatcgga ccaagctttg
     6781 cgtatacgcc aaattatcca attgacagta atcctgaaaa tgttctggct gctgaaaatg
     6841 cagaagacct gatggccaat tattggatgg atgtctatat gtggggaaaa tatccgattg
     6901 ctgccatgag gttcttagaa gaaaaaggat gggcaccaac gattgaagct ggcgatgcag
     6961 aattgctaga atctgctaaa ccagattttc ttgggatcaa ttactatcaa acggcgacga
     7021 atgcattcaa tccattaaac ggagttggtg ctggaaaaat gaatacgaca ggaaaaaaag
     7081 gttcatcaga agaaactgga gttcctggaa tgtataaaaa agttgagaat ccatttgtcg
     7141 aacgaacaaa ttgggactgg gagattgacc cagaaggatt aaggatcgga ctgcgtcgta
     7201 tcacaagccg ttaccgcatg ccggtactga ttacggaaaa tggcttagga gagtatgaca
     7261 agttgacaga agataaacaa atccatgacg actatcgaat caattactta caaagccacg
     7321 tcaaagccat caaagaagca atttcggatg gagcagaagt cctaggctat tgcacctggt
     7381 cttatacaga tttgcttagc tggctgaacg gttatcaaaa gcgatatgga tttgtatatg
     7441 ttgatcaaga cgaaacacaa gaaggatctt tggaacgata taagaaagac agttattact
     7501 ggtaccaaaa actgattgaa gaaaatggag agaaccttta gttcacatac ttaaaacaag
     7561 aaggtacggc tggatatggc cgtgcctttt cttgataaaa tcatttagaa attaagaaaa
     7621 actgtaaaat aagctgaaaa atgaaagaaa gcgtcagttc tttcaaacaa aactttagtt
     7681 cccttacttt tttttgtata atgaaacttg atgaaatgag ggataataaa tggcacaaaa
     7741 gaaacggcct actaaaaaaa ggaaaaagac gaaaaaaacg aaaaagcaaa aacagcagca
     7801 ggaacaaatg ctgacgattg gtgttgggtt tgccttgatt ttatttgctg tcttcggctt
     7861 tttaaaatta ggttttttag gaatattgat tgcaaacggc tttcggatca ttgcaggaaa
     7921 tacttaccaa atcctctgtc ttttattagc cgtattagga ttttggattg tcattaagaa
     7981 tacagagttt tcaattggta aaaaccgacg ctggtttggc ggcatactct tttatttcgg
     8041 tatcttatta ttgttacatg cccatttatt tggcaaactc catactgggg aaccaaatat
     8101 catggggacg acatgggatt tgttagccag tgatataaaa caaagtcaag tagacaataa
     8161 cgttggtggt gggatgattg gagcaatcct ttatcatttt acttacttct tgattgcaca
     8221 gcctggaagt tatttagcag cagtgctatt actggcagga ggagcattcc tgttcagtaa
     8281 tctggaaggc tatcagctgt taaatggtat ccaatcaatt ggagaacgag tacaggaatt
     8341 attggaaggt gatcctgcca agcaagctcg aaagcaagcg gcaaaggaag agagaatgaa
     8401 acagcgcgca gaagcgaaag aagcaaggcg cttagctgca aaagaagctg cagaaaaaga
     8461 agctgcagaa tatgagaaga aaaaagcggt gagccagcaa agggaaaata ctgctgatga
     8521 atggcaggaa ccagaaaata cagagccgga gcagctaagt tttgttccaa tcgacagttt
     8581 tcaagaaaat atccatcctg cgaatctaga gaaaccagta ccagatacac caaaacagac
     8641 caatacagca gaaggttttt cagatgagtt gccagaggat gacggtacct cattagaatt
     8701 tgagattgaa gcagaacagg aaaatcaaga ctatgaatta ccgacagttg atttgcttga
     8761 ttctatacca actgtggatc aaagtgatga gtataaaaaa attgaaaaaa atatcggcgt
     8821 attagaacaa actttccaaa gtttcggtgt cgatgccaag gtcgtcaaag ccagcttagg
     8881 accatccgtc acaaaattcg aagtccagcc ggctgtaggg gtaaaagtaa gtaaaattgt
     8941 gaacttaaca gatgatatcg cgcttgcttt agcggcgaaa gatgtccgga tggaagcacc
     9001 gattccaggt aaatcactga ttggtattga agtaccaaat ggaaagatca gtatggtttc
     9061 ctttagagaa atcatcgaag cacaaccaaa ccacccagat aaattactag aagtaccttt
     9121 aggacgtgat gtttctggac gggtacaaac ggctgatctt tcgaagatgc ctcacttact
     9181 cgtggctggt tcgactggta gtgggaagtc agtagcaatt aatgggatta tcacaagtat
     9241 attaatgcgt gctaagcccc atgaagtaaa actgatgatg atcgatccga aaatggttga
     9301 attgaatatg tacaacggca tacctcatct gttgacgccg gtcgtgacga accctagaaa
     9361 agcagcgcaa gccttacaaa aagtagtaca agaaatggaa gaacgatatg aaaaattcgc
     9421 ggcaaccgga gtaagaaata tttctggtta caatgaattt gttcaacaga aaaacttgga
     9481 aaacggaacg aaacatccaa ccttaccatt catcgtagtg atcgtagatg aattagctga
     9541 tttgatgatg gtagccagca atgaagttga agatgcgatt attcgtttag cgcaaatggc
     9601 tcgggcagcg gggattcata tgatcctagc tactcaacgt ccaagtgtgg atgttatcac
     9661 aggaatcatc aaagcaaatg taccttccag aatggcattt gcggtatcaa gcggtacaga
     9721 ttctcggacg atcattgatt ctaatggggc agaaaagctc ttagggcgag gagatatgct
     9781 attcttgcca atgggtgaga ataaaccgat ccgtgtgcaa ggagcattca tttctgacca
     9841 cgaagtagaa cgggtcgttc aatttgttac agatcagcaa gaagctcatt atgaagagaa
     9901 aatgatgccg acagatgaag ttgaaacgac aggtgctcca gaacagccac aagatgagct
     9961 gtttgaagaa gcaaaagcac ttgtcgttga aatgcaaacg gcaagtattt cccttctcca
    10021 aagaagattt cggatcggtt acaaccgagc agcacgttta gtagatgaac tggaagctca
    10081 tggagttgtt ggcccgtcag aaggaagcaa gcctcggaaa gtattcattg agcaggaaga
    10141 aacatcagaa acgttgccgg acgataatga catgtaaaac ttgtgtgatg aagggttgaa
    10201 aaacgaataa ccaataaaaa gacaaggcat gatgatacca aaagaaaaaa tggttttttt
    10261 ccatcttttc taatacggta tgatgtcttg tctttttagt ttttgtgaga ttaaagttct
    10321 attcgattta tttgtgaagt attgcattaa ggaaatgaat catgtatagt atgtagcgaa
    10381 actttgagaa aaagagggaa aatactttgg aaaaacagtc catttatggc ttgacgaatg
    10441 aagaattaat caattggttt atagaaaacg gcgagaaaaa attccgagct gcccaagttt
    10501 gggaatggtt gtatcaaaaa cgtgtaagta atttcacaga aatgacgaat ctatcgaaac
    10561 aattgatcga aaaattatcg gagcatttta tcatcaatcc tctaaaacag atggtcgtcc
    10621 aagaagcttc ggatggtaca gtaaaatatt tgtttgaact ccctgataaa aacatgattg
    10681 agactgtact gatgagacaa gaatatggct tgtcagtttg tgtgacgaca caagtaggat
    10741 gtaatatcgg ctgtacgttt tgtgcaagcg ggctgctcaa aaagaaccgt gatctgactg
    10801 ctggagaaat tgttgcacag atcatgatgg tccaacatta ttttgatgag cgcaatcttg
    10861 gcgaacgcgt ttcacatgtc gttgtaatgg ggatcggtga gccatttgat aattacgaca
    10921 atgtaatgga ctttttgcat atcatcaatg atgccaaagg attggcaatt ggtgctcggc
    10981 acattacagt ttccactagt ggattagcac acaaaatcaa ggaattcgct aataacggct
    11041 tgcaagtcaa tctggctatt tccttgcacg cgccgaataa tgaggtgcga acatcgatca
    11101 tgcggatcaa ccgaagtttc ccaattgaga aattgatgga agctgtggat gagtatttag
    11161 agaaaacaaa ccgtcgaatc acgtttgaat atatcatgtt gaatcaagta aatgatagac
    11221 cagagcatgc gcaacaatta gcagatctat taaaagataa gaaaaaatta gcatatgtca
    11281 acttgattcc atataatcca gtcagtgaac atgatcaata ttctcgcagt ccaaaagctg
    11341 atgtattgag attttatgat gtattgaaga aaaacggcgt aaactgtgtg atacgtaaag
    11401 aacatggtac agatattgat gcagcttgcg gacaattgcg aagcaagcaa atgaaaaaaa
    11461 cagttgccaa ataatcgtat cctactcgtt ttttcaatta ccataaaagg agcagttttc
    11521 ttaattaagg aactgttcaa cttaaataag agaaaagatt ctatagcgaa agctataaag
    11581 ctttttttga caagaaaaac cctcatcaag caaagaaatg tcctcaattt gatgacagcc
    11641 tgcttgatgg gggttttagc aattatttaa ctgattttct tatatccttg ctggctttct
    11701 tgtgtagcat gataagtttc tacgtcaaga atggcctgtt taaatttttc tcgggtgatt
    11761 tcataaggca catgtttaat atcatttgtt gccatggctt tatctaagat tgcttctaaa
    11821 tcggtcgtga cattgagatt catgtcggtt aacgatactg gaagttggtt ctttttcata
    11881 aacacataga ttttatcaaa caaatcgaac tgctgatcca tcatcaataa ggtcagtaca
    11941 ccataactga cagatgctcc gtgaaggtat ccctccattt tttctagaac agtcgtaccg
    12001 taatagaatg agtgtgccat agcagaatta tagttattgt taactaaaac agaaaccaat
    12061 ccggtagtaa caatgatatc cgaaacgact tgttcaattg catatgaggg acgatcgtcg
    12121 gcactatctt tcaatgcttg cgtaccataa gtcaagagac gatcaatgca catttttccg
    12181 atttggattc ctaatctatc cgtgtagcct agatcatcgc cacgagcaga aaaagtagat
    12241 tcgacttctt tggataaagc atcgccaata cctgcccata agtatttatc aggtgcttcg
    12301 gcaataattg ctgtatcaat gaaggtgtgt acaggtggtt ttgaaggaaa gaagagtccg
    12361 tccattacgc cttcttcgtt atagatgaca cataccgcag tgacgggcga acagttcgaa
    12421 ccaatagtgg gaaaggtgaa gactggttta gctagttttt caccaatgac ttttacagta
    12481 tcgcaagcac ggccaccacc gactgcaaac aggacgtctg ctttttggac actttcatgc
    12541 tggatcaact gttcaatatt tccataagtt gaattacccc cgtaccagag actggcgaca
    12601 acctctattt cctgtgtcaa agctactttt attttgtttt ctgcctttga aagtgcagtc
    12661 ttccctccaa tcagcactac ttttgtccca tattctttga cgattccatt tgctttttca
    12721 tacgcatctg ttccaatggt ataatttggt aagaaaacac tttgactcat attcatttcc
    12781 ttctttcttc gttgaaacgg gttaaaattt taaagagtga tcgatctgac tgcggctgta
    12841 gatggagtag aggtgaacct aagacaggat aagtgatgaa taaggtggca tgaacacctt
    12901 gaaatcatca ataagtatcc taaaaacgaa caagtacaaa aacattgaaa acgacaataa
    12961 tgaatttttg ttcctcttag tatttctacc gattcccttt gcttttttgt aattatctaa
    13021 aaattctaaa aattaagatt attatatgaa aacaggtttt atttgtcaat tcataattgg
    13081 ttttttttaa agcaaaagat aatctatatc atgatgaaca aacagagttt tttcctccaa
    13141 ttgatggctt tcctgatctt tcctattttg aataaagtgt ttctgtataa tttttttatt
    13201 ttataagtaa gataaataat gaaagaatag agatactgat tggtagctaa agatttttga
    13261 gcgagaacat ttcgtttttt caaaaggact gttagaataa gcaagaacaa gggggaagac
    13321 gtatgataat cagtgaattt gaccgtaata atccagtatt gaaagatcag ctttctgatt
    13381 tactgagact gacttggccg gaagaatatg gagacagctc ggcagaagaa gtagaagaaa
    13441 tgatgaatcc agaacgaatc gcggtagcag cggtagacca agatgagtta gtaggattta
    13501 ttggtgcaat ccctcaatac ggtatcacag gttgggaatt gcatccatta gttgtagaaa
    13561 gctcccgacg aaagaaccaa ataggtactc gattagtcaa ttacttagaa aaagaagtag
    13621 cttccagagg aggaatcacg atttatttag gtacggatga tttagaccat ggaacaacgt
    13681 taagtcaaac ggacctgtat gagcatacat ttgataaagt ggcttccatc cagaaccttc
    13741 gtgaacatcc gtatgaattc tatgaaaaat taggttataa aatcgtaggt gtcttaccaa
    13801 atgcaaatgg ctgggacaaa ccggatattt ggatggcaaa aacgattatt cctcgaccaa
    13861 attctcaata ataaaaagag gttgtgacag aagtaatcag cttcaagaaa taagaaggaa
    13921 tttacgaaaa ttacttcttg aatttttgtg aaattccaac ttatttccga agaagctact
    13981 tttggaacac cgtgtaccag aggttgtgaa aaagtcgctt agtttcgagt agtaagaaaa
    14041 attttgtgaa aatggctttc tacatttttg cataaatttg gcttaatacc gagaaactgg
    14101 cttttgaaca ccgtttattc agataaggga ctgtgacaag acttttgtca cagtttcttt
    14161 ttgtttattc ttttttatct ttgtatggca ataatagaca atataacgaa aattatttaa
    14221 tggcttttgg aagaccatgg aaaaccttgt acaaagctat tttttctgaa aaattccttg
    14281 aaacctcacg aaaaaatcat tattttatga aaaagacaaa aaaaagtgtt gattttttaa
    14341 taattattca ttaaactctt aaacaacttc agaaaatatt aattattctg aaaattccaa
    14401 taaaggggta cgtaaaatga aaaagaaatt tacatttggt ttggttgcgg tatgcggcct
    14461 tgtacttgct ggatgttatg gcggcagttc agccacaagc agtagttcag caaaaagcgg
    14521 ttcagcagat ggaggaggcg tatttaactt agtcgtgcct caagaaatgc ctacagctga
    14581 cttatcagtt gcaacagata caatcagttt tacagcattg aataacgttt atgaaggaat
    14641 ttaccgtctt gatgaagaca gcaaaccaca gccggcagga gctagcgagt tggcagaagt
    14701 cagtgaagac ggattaactt acaaaatcaa attacgagaa gacgcaaaat ggtcaaatgg
    14761 tgatcctgtt acagcagcgg attacgttta tggctggcaa cgaacagtag acccagctac
    14821 agcttctgaa tatgcttacc tgtatgcgcc agttgaaaat gcagaagaca ttacagctgg
    14881 aaagaaagat aaatcagaac tagggatcaa agcagttggt gactatgaat tagaaatcaa
    14941 attgacgaaa caaacaccat atttccaata tctgcttgct ttcccatcat ttttcccaca
    15001 aaaacaatcc gtagtggaag aaaatgggga tgcctatgca tctgcaagtg ataaagctgt
    15061 ttataatgga ccattcacat tagaagattt tgatggacca ggaacagata cagaatggac
    15121 ttataagaaa aatgatcatt actgggacaa agatacagta aaactttctg aaatcaaagt
    15181 cagtgtagta aaagaatctt ctactgcatt gaacttattc aaagatggcc aagcagatga
    15241 cgtgatcttg tctggtgagt tagcacagca aaacgcaaac gatccagctt atacttctgt
    15301 taaagaagcg cggacaagct atatcgaatt caatcaacgt gaaaagaatt caccattcaa
    15361 caatgtgaac ctaagaaaag ctatttctta ttctatcgat cgtgaagctt tagtaaaaca
    15421 agtactaggt gacggatcag ttgtctctac aggattgatc ccatcagata tggtaaaaag
    15481 tccaacaacg aacgaagact ttgcaaaaga agctggaaaa ttagtttctt atgacaaaga
    15541 taaagcaaaa gaatattggg aaaaagcgaa aaaagaattg ggcgttgatt ctttagaatt
    15601 tgatttgatg gcttctgatg atgattcatc gaaaaaagta atcgaatatg ttcaaaactc
    15661 aatccaagaa aacttggatg gcgtaacagt caaaccaaca cctgttccat tctctgtacg
    15721 tcttgaccgc tcaacttcgg gtgattttga cgcagtacta ggcggatggg ctgctgacta
    15781 tgcagaccca agcagcttta ctgatttgtt tgtcacaggt aattcataca accgtggtcg
    15841 ctggagcaat gcagaatatg acgaagcagt taaagcagct tcatcaacag acgctggcga
    15901 tgaacaagcc cgttgggatg atttccaaaa agcaatcaag atcatctctg atgatatggg
    15961 tgtcgttccg gtgtaccaaa aagcagaagg tcacctagtg aatgaaaaaa tcaaaggtat
    16021 cgttcaccat gcggcaggcg cttcatggga ttacaaatgg acatacgttg aagaataatc
    16081 gttaataccg tgagcatgag aaaaaacgag gctgcggcaa atgtcgcaac ctcgttctca
    16141 tattcactta tcctaacaaa gaaaaaagca tgaaaatagc agaaagagca attagtattc
    16201 cggcaatgat cagccaaaag ctgtacatac cgtagctggc agacgagagc gaggaaaatt
    16261 ctggtttttt ctttcggttc cttgttcgtg caagatggac cgatcgctga aaatgatcga
    16321 agaaacccga agaaaagacc cataaaaaac cgccgataat cagaaacggc aatgaccata
    16381 agaatagatc attagataat tgaatgagtg taaaggaatg gttgatgaga ttttttatta
    16441 aaataaccag aatcagtaga aaagctactg cgtaggggat agattttttt ttcatttgat
    16501 tgctccttta tttttggttc attatctaat aatgtagcga aacatctgtc agaagtcaaa
    16561 agctaggggg aaacattagt gaatagttat atcaaatatg tattaaaacg tgtctttttc
    16621 atgatcatca cgctttggtt aattgcaaca atcacctttt tcttgatgca attgctgccg
    16681 ggcacgccat atacgaatca ggaacgtttg agtcctgaaa caatcgagat gctaaataaa
    16741 caagtcggtt tggataaacc agttatcgta caatatggga tttatctttc taatttgctt
    16801 caaggagatt ttggaatttc cttccaattt aaaaaccagc cagtcgcaca tttgttagct
    16861 ggaagaatcg gaccatcctt gcagttaggg ttacaggcaa ttatctttgg aacagtccta
    16921 gggacgattt taggaacgat ctctgccatg aagcaaaata catgggcaga tacatcttct
    16981 acactcgtag cgatattagg tcgttccatt ccaaactttg tatttgctgt tttattacaa
    17041 tatatctttg caatcaaatt acaagttttg ccaatcgcaa aatgggatgg ctttgtgtac
    17101 acaatcttac caacaattgc gttggccatg tcgccgcttg ctgactctgc tcggtttatc
    17161 cgtacggaaa tggtggaagt actgcatagt gattatgtgg aattagcaaa agcaaaagga
    17221 ttgagccgtt gggaaatcgc ctttaaacat ggtcttcgaa atagtttgat tccattgatg
    17281 actttattag gaccgttagc tgtagcattg atgaccggat cactggtggt agaaaatatc
    17341 tttgctattc ctggtatcgg ggaacaattc gttaagtcaa tcacaacaaa tgattatcct
    17401 acgatcatgg ctgtaacgat cctttactca tttatgctga tttttgttat tttgatcgtg
    17461 gatctattgt acggtctagt tgatccaaga attcgtgttt cagaagggag ccgaggataa
    17521 atggaaatga taccaaagcg ttatgaaaca gttgcagata tccctgcgga tgaatttctt
    17581 ccattacaaa aaaataccga agaagaacgt gaacagatta ctgcaccttc tttgaatttt
    17641 atccaagatt catggcgccg tttaaagaaa aacaaagctg ccgttatttc aatggtttta
    17701 ctattggtca tcattttgat ctctgtgatc acaatctttg tttctcctca tgatccaact
    17761 gcccaaaacg tggcttatat caatttgcct cctcgtattc cgggaatcaa tatcgatgga
    17821 ttgaatggaa aagcaatggt tggtggtgaa cttgttgata aatatgctca agcaaacgtc
    17881 ccttcaaacg taaatttctt tttaggaaca gacggtttgg gacgtgatgt attaagccgc
    17941 ttatttatgg gaatgagaat ctcgttgctg atcgcattta ttgctgcatt actagatgta
    18001 acgattggtg tagcttacgg gttggtttct ggtttgttag gcggacgtgt cgataacgtg
    18061 atgcaacgtt ttttggaaat cttatcagga attccaaacc tcgttgtcat gattttgatg
    18121 ttggtcgttt ttgaaccagg aattttttca atcgtggctg ctatggccat cacaaactgg
    18181 ataccaatgg cacgtatcgt tcgtgcacaa acaatgaaat taaaagatca agaatatgtt
    18241 ttagctggat tgacattagg tgaatcaaga atgaaaattg cattcaaaca tattttgccg
    18301 aatatctcta gtgtcattat tattcaaatg atgtttagta ttccatctgc tattttcttc
    18361 gaagcctttt taagctttat cggtttaggt cttacgcctc cgtcagcttc attaggaacg
    18421 atgttgagcg aagggtacaa gacattcctg tatctgcctt atctattatg gattccggca
    18481 gtaactttat cggttatcat gattggcttt aacttacttg ctgatggatt gcgtgatgcc
    18541 tttgatccta aaatgaaaga gtgaagacga tgacgaaaat tttagaagtg aaagacttag
    18601 aaatctcttt tgatacgtat gctggaaagg tacgagcgat tcgcggtgtc aattttcatt
    18661 tgaataaagg agaaacattg gcgatcgttg gggaatctgg aagtggaaaa tcagtgacga
    18721 ccagaagcat catgcgcttg ctgtcaagca acgccaatat agatgctggt caaatcctct
    18781 ttaaaggtaa cgatatcgtt ggaaaatcag aaagagagat gcaaaaaatc cgtggtaaag
    18841 aaatagcgat gattttccaa gatccaatga cctctttaga tccgacgatg cccattggga
    18901 agcaagtcgc tgagtccctt agaaagcata ataaagtttc aaaaaaagag ggacaaaaag
    18961 ctgcattaga attgcttaaa ttagttggaa tacccgaagc tgagaaacgt atcaacagtt
    19021 atccgcatca gttctcagga ggtcagcgtc aacggatcgt gatcgcgatt gcactggtat
    19081 gttatccgga aatcctgatt gctgatgaac ctacaacagc gcttgatgtg actattcaag
    19141 cacagatttt ggagctgctg aaagaaattc agacaaaaat cgatacatca attatcttta
    19201 tcactcatga ccttggtgta gtagcgaatg tcgctgatcg tgtcgctgtc atgtatggag
    19261 gaagaatcgt tgaagttgga acatcggaag aaattttcta caacccgcaa catccctata
    19321 cttggggact gcttggttca atgccgacat tagatagtgc gaacgatcga ttgtatgcta
    19381 ttcctggttc tccaccggat ttattgaacc cgccaaaagg agacgcattc tatccaagaa
    19441 atgaattcgc gatgaaaatc gatgcagaac aagaaccacc attttttgaa ctatcaaaaa
    19501 cacacaaggc agcaacttgg ctacttgctc cgcaggcacc caaagtgaca ccgccagaag
    19561 aaatccaacg tcgctgggct atctttaaag agaaacaaaa acatacttac ggtcaaggag
    19621 gaattgctga tgccaaataa actacttgaa gttaaaggat tgaaacagta tttcaatgta
    19681 ggaaaaaaga atgaagtaca tgctgtaaat gacatcagct ttcacattta tgaaggagaa
    19741 acattcggtt tagttggaga atctggaagc ggaaaatcca ctactggccg tacgatcatt
    19801 cgcttgaatg aacctactga tggtgagata ttattcgacg gtcaagatat aacgaagatc
    19861 aaagataaaa aaggtttgac gaaattccgg catgatgttc agatgatctt tcaagatcca
    19921 tacgcttcgt tgaatcctcg tatgaaagtc agagatatca ttgccgaagg aattgatgtt
    19981 aatggactag ctaaatcatc tgaagagcgt gcaaaaaaag tcgatgatct attgagaact
    20041 gttggattga atccaagcca cggtactcgt tatcctcatg aattttcagg tgggcaacgc
    20101 cagcgtattg gtattgcacg agcattggca gtaaaaccgc gttttatcat ttgtgatgaa
    20161 ccaatttctg cactagatgt gtcgatccaa gcccaagtgg tcaatttgtt gcaagatctc
    20221 caaaaagaac atcaattgac ctatctattt attgcacatg atttgtcgat ggttaaacat
    20281 attagtgacc gaatcggtgt aatgcataac ggcttgttat tagagatggg aacaagtgat
    20341 gaaatctata atcacggtgt tcatccttat acagaaagtt tgttatctgc tattcctcta
    20401 ccagatccag accatgagag aaaacgcaga agaatcaaat atcaacctga accagatgac
    20461 ggacaagtac gtcaacttcg tgaaatcgca ccagaacatt ttgtctatgc aactgagcag
    20521 gaagtcccat attacgcaaa aaaactaaaa cgacaaaagg aatctcttct ggttgctaac
    20581 taggagggac atagatgcta aaagctttta aattccgtat gtatccgaca gaagaacaaa
    20641 aacagcaatt gattcgtact tttggatgtg ctcgatttac ctataaccat ttgctgaaaa
    20701 agcggcagaa aagttggcaa caaacaggag tagccaattt ttcgctcaca cccgctacat
    20761 taaaaaaaga atacccattc ttaaaagaag tagacagtct tgctttagca aatgcacagc
    20821 ttaatttgga tcgggctttt cgcaattatt tcaagggacg tgccagcttc ccaaagctta
    20881 aaacgaaaaa aagtatgtgg caatcttata cgaccaacaa ccaaacccgt acgatctatc
    20941 tggaaaatgg ctacttaaag ttaccaaaac aaaaagaact aatcaagata aatcagcatc
    21001 ggccagttga aggatcgatt cgctccgcta cgatttctgc tcgatataat gaagaatttt
    21061 atgtcgcact actttgtgat gtttcgccag taaaaaaaga atctttagct aaatggatcg
    21121 ggatcgccta tcatccgaag acattgatcc aaacatctcg acctctagaa gtaactttac
    21181 caaaatttca ccagacagaa gaaaagctgc aacatgcaca gcggaaattg aatgtgaaag
    21241 tccgctccgc tcaccataga aagatcaggt tagatcaggc aagcaattat cagaaacaaa
    21301 agagaaaagt aatggatctt tatctgaaac aaaagaacca gcgagaagat tatcttgaac
    21361 aattatctgg gaaattggtc aaacaatatg actacttatt tgtagaatct tttccaaaag
    21421 aagaggcaca tgctgatttt tctattcacg actggcataa attaataaca aaattgcgtt
    21481 ataagtccca atggtataat aaaaaatttc ttttgatcaa taccgacgga gcagaagaaa
    21541 gcaattccgt aaaaaagagc caagtagtag aaaaaatggg cagacattcg gttatcaagg
    21601 gataaaaaat gaatgaaact gataggagcg ttagggttag ccttgttaat aaaaagtctt
    21661 aatgactttt gtttctagga atttttcttt tgaagaaaag ctcactaatt atgacaaacc
    21721 aataaaacaa tagcagaaaa agttcgtacg agctttttct gctattgtta attttttatt
    21781 cagaaagttt acgaatacat caaaattctg aaacaaaaaa cgtaactcac aaagagttgt
    21841 ttttgcgttt tttcttgaat gattaggtct gaatcttttg ctcacaacct tattcatggt
    21901 gccccggctt gagctgatag atttttgaag gacggccgat tcctacagga cgttcaccga
    21961 tctcatcgaa atattggagc atagctttct taaaattgga atgatcgatc gttttataat
    22021 cgatccctaa gaatttagca aatacttttc gagcttccgt aatcgtgaaa tctttaccta
    22081 aaacttgtag aacttgcggc tcgtgttcca ttttattgac tacacggttg aatgctttca
    22141 agatgatttg actatggtca aaagccaaag tatctttgcc agaagactct ccagtgacta
    22201 aatcaagcga gatagccact tctccgctag ataaatggat cttgttacta tgacgttcta
    22261 gtgtgaacca gcgtacttcc ttggcatcat ctccggcgat tagcggttct tctccaataa
    22321 aggctaagta gctgactgtg acgacccagc ctcgcggatc tcgattaggc gtactgaatg
    22381 tatgcagttg ctcaatattt tcttttgaaa taaccacacc agtttcttcc tttgtttctc
    22441 ttaagacgct ttctccagtt gactcatcct tttggacaaa gccaccaggc aatgcccatg
    22501 aatttcgata aggatgccct tttctttgga tcaacaaaac tttcagctga tcttcttctt
    22561 tgttatagca catcagtacg atatcgacag tcaaagaggg cttgtcatat tctggacgtt
    22621 cctgtttttg ataccagtta agaaattctt cttcacttgc tttatgttca taatataatt
    22681 tttcttcttc tttagaagca aaacggttca tctgaccacc tccattgaca gtaaaagtac
    22741 tttttacctt attgtagcag actctgaaaa aaaaccaagg atttacttga aaaatctagg
    22801 ttatcattta aatatgaaaa ataaatggaa gtatgagatt gaataaaaag aaaagcagat
    22861 aaaacggcaa tactttatac cgctcttcac gaatggcaaa gaagccacaa ataacgatat
    22921 atacgtcaat gataaaacgc aaaatccaag ctgctgtcat aaaactgttc aaatgcccat
    22981 ggaggttaaa ataaccatac atacgaatat aaaaatagct gaatattaaa tgtgctaatg
    23041 aaagaaaagc aatactaagt gtaaaaaaaa gatgtttctt ctgcatgatt gttccagtcc
    23101 cttactaaga tagatagtct ttattatacc acgtgtgagg ggagaaataa aaagccgttt
    23161 tttagtagat atgaaacaaa acaaaaaccc gcatttattc aacctattca tccttttaaa
    23221 ggatagattg aacgtagata gaagtcgttt atccagtctt tttcattttg aaaaaaagct
    23281 aacagcatgc tttttttatg gtatactagc aaggaaacga attcgcatca tgcgataaag
    23341 gaggaagagc cccgtggcag aaaaggaaac tttttatatc actacgccaa tctattatcc
    23401 aagcggcaaa cttcatatcg gcaactcgta tactacgatt gcatgtgacg caattgctcg
    23461 ttataaacgg ttgatgggct ttgatgtgtt ttatctcact ggagtggatg aacatggtca
    23521 aaaaatcgaa acaaaagcag aagaactagg tgtaaaaccg caagaatatg tagataaaat
    23581 ggcggctgat gtcaagaaat tatggaaaac gcttgatatc agttatgata aattcattcg
    23641 cacgacagat gattaccata aagcagctgt tcaaaaaata tttgatcgtt tattagaaca
    23701 gggagatatt tacttaggtg aatatgaagg ctggtactct gtttctgacg aagaattctt
    23761 tacagaaaca caacttgcag aagtgtacaa agatgacgaa ggtaacgtga tcggtggtaa
    23821 agcgccaagt ggacacgaag tagaattggt aaaagaagaa tcatacttct tccgcatgag
    23881 caaatacgca gaccgtctat tggaatacta tgaagaacat cccgaattca tccagccaga
    23941 atctcgtaaa aacgaaatga tcaacaactt tatcaaacca ggattagagg atcttgcagt
    24001 ttcacgaaca acttttactt ggggagtacc agtcaagagt gatccgaaac atgtggtgta
    24061 tgtatggatc gatgcattat ccaattatat tactgcacta ggatatggct ccgaagatga
    24121 aagtctattc gaaaaatact ggcctgcaga tgttcagatg gtcggtaaag aaatcgttcg
    24181 ctttcatacg atctattggc caatcatgct gatggccctt gatttgcctc tacctaaaaa
    24241 agtatttggg catggctggc tattgatgaa agacggaaaa atgtctaaat caaaaggaaa
    24301 tgtcgtttat cctgaaatgc ttgtggaacg ttatggttta gatgcactcc gttattattt
    24361 gcttcgtgct gttccgtttg ggtcagatgg tgtctttacg ccagaagatt ttgtttcccg
    24421 tgtcaattat gatctggcaa atgatctagg gaacttattg aatcgaacga ttgctatgat
    24481 caacaaatat tgtgatggac tcgttcctga ctatgcctca cttgtcactc catttgatag
    24541 tgaactgtct acgacggctg ctaacgttgt cggacgttac catgatgcaa tggaaaaaat
    24601 ggaattcaat acagcattgg ctgaaatctg ggtgctgatt tctcgtgcaa acaaatacat
    24661 tgatgaaaca gaaccatggg tattagcaaa agacgaagaa aagaaagctg aattgaatag
    24721 tgtcatgatc catttagctg aatctttgcg tatcgcagcg atattacttc agccaatcat
    24781 gacagaaacg ccagagaaga tcttcaatca actaggattg gactctgaaa caatgaatct
    24841 tgaaggatta cactttggcg aatttccttc aggaacaaaa gtagttgcaa aaggtacacc
    24901 aatcttccca cgtttagata tggaagaaga agttgctttt attcaagaaa aaatgtctga
    24961 aggtactcaa acaaacgaag atacagtcaa atgggatcca gaagaaacag aacttgtttc
    25021 aacgaaagaa aaacaaatca aatttgatgt atttgaaaaa gtagaactaa aagtagcaga
    25081 agtcatcaac tgtcaaaaag tagaaggggc agataagttg ctgcaattcc gactagatgc
    25141 tggagacagc caagaccgtc aaatcttgtc aggaatcgct gaattttatc ctgatccaag
    25201 cgaattgatc gggaaaaaag tcgtcattgt agcaaatctg aaaccacgaa agatgcgtgg
    25261 acaaatcagt caaggaatga tcctttctgc agaagcacca gatggctctt tacaagtaat
    25321 cgaagcacca aaatccatgc caaatggctc agaaatcgca tagaaattca aagggggtat
    25381 gacactagtt gtgtcatacc ccctttttcc gaaatttgag aagcaatttt cgtaaattcc
    25441 ttcttattac ttgaagccaa tcatataata cagaaacaga gaactatcca aaataaatcc
    25501 tagaaaaaga agtacgagct tcgctgattt ttgttaattt agaataatac taataggaat
    25561 cgtaaaaaca gaaaaaaagc ataatgataa agggtcgggc agattttaat tgattcatct
    25621 ggtactcttt tttattgtcc ttacattcat aaagtttgaa tgcaatgact ttattacatc
    25681 tatatttata tctaacagta gcgttgatgt gttaattata agttctatca ctttatgttc
    25741 aattagtttg tgctcttata tgtgtgattg aaaatataga taaaaaaaca atcatcggct
    25801 ttgacttcga tgattgtttt ctttattttg taaaaatcct atcacttgat tttatggtaa
    25861 ccttttattt ttgtcttcaa acggcttggt aagagagcag taagcagtgc aaatgggatc
    25921 gaaaagacat accagaaaat cgtacgtccg ataccataat gttgatagag ctttgtcaac
    25981 atcacgtgta acaagatcaa ccctaaaagc aaggagccag ctataaacca atgattatcc
    26041 caatgcatga cttcactaag tgccatcact ccaaaaggat agaaataaaa atagatcccg
    26101 actaaggtca tccgccagtt ctttgtaaat gttccgatat tcaataaaaa gaaaatcgct
    26161 gaaagcaaaa taccgatagg cggaaaaagc ccaaccaaaa aggaatacaa catcccccac
    26221 aagatcatca caggaccttt gatcaatgca acgattgcga tgaacaaagt cagcaatatt
    26281 agtttttgtg ataacgttgc aacagtcatt aatccaaata aaattaatgt gaaccaatgc
    26341 caatgggact gctgttgttc tttacgggaa aaatcgaccc ggttggataa ataatctgct
    26401 aatttcggat aagaacgctt aaaatccata aactgttcct ccttcgtgga aaaggtaggt
    26461 ttagtatacg aaaaataaaa aagaaataca tccgtcttaa gagggagttt ttcactgaaa
    26521 gtggagaaaa aagaatggta aattaaaata ttctcagtta tactgaaaat tataccaaat
    26581 ttcaaagaag aaagaggatg ggaatgaaag gcaaactaaa acgagaaatc aatctgtttg
    26641 gcgcattagc taccgttatg ggaaccgtga tcggtgcagg cgtcttcttc aaaactgctg
    26701 ctgttactgc tagtactcag tctacaagtt tgacattgct agcttggctt ttgggtggtt
    26761 tcttaacgat ctgtgctgga ttgacagtgg ctgaattagc tacagcaatc ccggaaactg
    26821 gtggagctgt aaaatatatt gaagctgcgt atggaaaatt gcccagcttt ttattaggat
    26881 gggcgcaaag cttgatttac tttcctgcta atattgctgc attatcgatt atctttgcga
    26941 cacaattgac gaacttatta caattatcta ccgattatct tttgttgatt gcagtgatca
    27001 ccgctgtttc tgtgacaggg ttgaatttgt tagggacaaa agtcggtgct tctgtacaat
    27061 cagtgacgtt gattgttaaa ttaataccaa ttgctgtgat tgtcatatgg ggattattga
    27121 ccccaggaca aggaactgtc cagttatttc caatcgaagc tggaaaagat gttacttttg
    27181 tcgaaggatt gagcagtgcg ttgttagcaa cgttgtttgc ttatgacggc tggttaggtg
    27241 tcggtgcaat ggcaggggaa atgaaacggc cggaaaaaga tttgcctaaa gcaattattc
    27301 ttggtttgag ttttgtaaca gtcgtttatc tattgatcaa ttttgttttt ttgaaaactt
    27361 tgccgattga tcatcttgcc ggtaatttga atgcagcttc tgaagcatca gacgtcattt
    27421 ttggaggaat cggtggaaag ctggtcacta ttgggatctt gatctcggta tacggtgcat
    27481 tgaacggtta tacactaact gggatccgcg tgccttatgc gatggcttta gaagacgagc
    27541 tacctttcag taaacagctg acaaagcttt caaagaagtt tacggttcct tatgttcctg
    27601 cggttttcca gttagcagta gcttgtatta tgatgagcct tggttctttc gactttttaa
    27661 cagacatgtt gatttttgtg atgtggctat tcagtttact gatttttatc ggtgtcttta
    27721 ttttaagaaa aaaagcgccg gaattgccac gtccatataa agtaccactt tatccaatcg
    27781 ttccgattat tgcgattctc ggtgctattt tcattttagg tatgacgatg atgacgcaaa
    27841 cgaaattagc attgatcggt ataggtgtta ccttgatagg tatcccagtc tattatcaaa
    27901 aaaagaaaaa acgctcagaa gagtaaaata tttcaacaac agacagaaaa agtacgaact
    27961 aggtcctcta gatcttcgtg ctttttctat ttcaataaaa caagttcttc tattttgatt
    28021 tttgctactt ctgcaagtag tgcttatgtg ctaaagtaag aatgaaaagg agagataaac
    28081 atgatatttg attctcatac ccatttaaat gctgaacagt tcaatgaaga tattccagaa
    28141 acgattcagc gagcacaaga aatgggggta acgaaaatgg cggtcgtggg cttcgatacg
    28201 ccaacgattg aaaagtcttt gcaattaagc catgactatc caaatatata cagtattatc
    28261 ggctggcatc cgacagaggc tggcagttat acgaaagaaa tcgaaaataa attacaagaa
    28321 caattgactt taccaaaggt tgtagcatta ggggaaattg gcttggatta tcattggatg
    28381 gaagacccga aagaagtcca agataaagta tttcgcagac agattgctat cgcaaaagaa
    28441 atgaacctac caatcagtat tcatactaga gaagcacttg aagacacgta tcaaatccta
    28501 aaagaagaag atgttcgaga tatcggtgga atcatgcata gtttcagtgg tgattatgaa
    28561 tgggcaaaac gatttcttga cttagggatg catatttcat ttagcggtgt cgtcactttc
    28621 aaaaaagcat tagacgtcca agaggctgca gcaaatgtac caatcgaacg attacttgtg
    28681 gaaacagatg ccccttatct ggcaccagtt ccttttagag gaaagcgaaa tgaaccaggc
    28741 tatactcgtt atacagtaga aaagattgct gaacttcgga atcttccgac agaagaagtg
    28801 gctgcccaaa catggacaaa tgcgcaccga ttatttggtc taagcgaaca tggataagat
    28861 gaagatccaa gagattatag tcgttgaagg aaaagacgat acaagaaggc tgcgggaagt
    28921 agttgatgca gatacgatag aaacaatagg gtctgcaatc aatgaagaga tcctgatgca
    28981 aattgaacat gcccaagaga ctcgtggagt gatcgttttt accgatcctg atttttcagg
    29041 agaaaaaata agaaaaatca ttatggaagc tgttcctcag gcaaaacatg cttttttgcc
    29101 gcgcacacaa gctaaaccaa agaaaaaggg aagccttggt gtcgagcatg caagtgatgc
    29161 tgctatttta gaagcattga aaaaggtcgt gacgccagac atttcccaaa cggctgtttc
    29221 tgaaatcact agacaagacc ttttagacta tggtttaata gctggggcac aagcaaaaga
    29281 aaaacgagaa aaactaggtg acgagttgcg catcggttac acgaacggca aacaattgga
    29341 aaaacgtttg aagatgtttc gtattacgcc gaaagaactc gcccaagcaa tgaaaatagt
    29401 ggaggaacaa catgagtgaa tatcgtgaaa ttgcgacacc ctctagaaca aaagagattt
    29461 tattgaaaca tggtttttca tttaaaaaaa gtttaggaca aaattttctg actgaaccaa
    29521 atattttaag gaaaatcgta gaaactgcgc aaatagatga acatacgaac gtgatcgaag
    29581 ttggaccagg aatcggtgca ttgacggaac aattagccaa gcatgcaaaa caagtcgtag
    29641 catttgagat cgatgatcgt ttgatccctg tattggctga tacgatgcaa ccctatgaca
    29701 atgtgactat tgtacatcaa gatattttaa agaccgattt gtcaacagct gttagagaaa
    29761 catttcatga ggagcttccg ttaaaagtcg tagcgaattt accttactat attacgacac
    29821 cgatcatgat gcattttctt gaatcagatt tggttgtgga tgaactagtc gtcatgatgc
    29881 aaaaagaagt agctgatcgt atctctgctg aaccaggaac gaaagcttat ggttcattat
    29941 cgattgctgt tcaatattat atggaagcca gtttagcatt catcgtacca aaaacagtat
    30001 ttgtccctca gccaaatgta gattcagcga ttttgaaact gacaagaaga gatataccgg
    30061 cagtcgaagt gacagacgaa aaagcatttt tccgattgac aaaagcggca tttcagcaaa
    30121 gaagaaaaac gctttggaat aacttgcagc attcgtatgg caaagatgac cagacaaaag
    30181 catggttagc aaaaagcttg gaaacagctg gaatcgatcc aaaacgtcgt ggggagactt
    30241 tgtcattaca agaattcgcc gcattaagca atgcgatgtc agaaaatatg cagtaaaaaa
    30301 agaggttgtg acagaagttg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa
    30361 caaaaatagt tcctcatgtt tttcgtttgt ttgggcttat taccgcagat gcaagctttt
    30421 gaacaccgtt taagaaagag gttgtgggcc tgccgtttaa ctctaagtca caaggaacaa
    30481 tttcaaaaaa cagcttctca tgtttttgag ttagttggtc ttattacttg atgcaagctt
    30541 ttgaccacta ttatgccgaa caaggagatg tgacacaaat tttgtcacat ctcctatttt
    30601 tgtttcttga tcaaaaaatt cttatacttg atactcacac tcattttgta tggatttaga
    30661 aaagaatatg tgtcttgaca gttattagta agtgagttat actaagtcga catttatgtg
    30721 tcaagacata ttaggagaga gaaaaaatga gaaaaaattc agttcgaaaa ctaacgattt
    30781 ctgctttatt gattgggatg ggggtcatta tcccaatggt catgcctaaa atcatgattc
    30841 caccagcttc ctttactttg gcgtctcatg tgccattatt tattgcgatg ttttttacac
    30901 caggagttgc agtggctgtt gcgctaggta cgacatttgg tttcttttta acaacgccgg
    30961 ttatcatcgc attgcgagca ttgtcacatc ttgtgtttgc gttgattggg gcttggtact
    31021 tgcaaaagca tccgagtatc gttttaaaaa atggacagtt tacttttgct aattggcgtt
    31081 tccaagggtt caattttgtg atcggtgtga ttcattcttt agcagaaatg ctagtagtca
    31141 gcatctttta ctttattggt aatatgcctg aaacatatta ttctcaagga tatttttata
    31201 cagtattcat cttgatgggg attggtgggc ttatccacag tttagttgat tataatattg
    31261 cttattttat agcaggaaca ctcagcaaat attttgatat ccccgtattt acggcagcaa
    31321 aaaaagaaaa tcggaaagag tcgattcaag ggtacgcgaa gaaaacgaca tattagacat
    31381 gatctctcaa aaaaaaaact ctcatctagt aatggataat aaatcctcta agatgagagt
    31441 tttttgtcct aaaaagagct gcaccaacat cttctggcac agctctaaca gggggcaatt
    31501 tatggaagtt tgatgacttg ttctgtttct ttatcaaaga agtggccttt gctgatgttg
    31561 aaggcaaggt cgactatttc gcctggttta tggaaatctc ttgcatctac ttttgagatg
    31621 aactcagtag aaccagtttt tgtgtaaagc atcgtttcag ctccaagtaa ttcggaaacg
    31681 acaacttctg atttcactac ggcttcaggt gaagcttcaa gggctacttg ttcactgtgg
    31741 atgtcttctg gacgaatacc gaagataact tcttttcctt cataaccttt ttcaactaat
    31801 actttgtttt ttccttcagg aatacgaagc ttaagcccat gtccatctgt gataacacca
    31861 tttttcaatg tcacattaaa gaagttcatg gcaggagatc caataaagcc ggcaacgaac
    31921 atattgacgg gtgtatcata aacttctttc ggtgtaccga tttgttggat aaatccgtct
    31981 ttcatgataa cgattcggtc agccatagtc attgcttctg tctgatcgtg agtaacatag
    32041 atggtcgttg tttcaagacg ctggtgaagt ttggcaatct cagcacgcat ggctacacgt
    32101 aatttagcat ctaaatttga taatggctca tccattaaga agacttttgc atcccgaacg
    32161 attgcccgtc ccaatgccac acgttggcgt tgtccaccag ataaagcagc tggttttcgt
    32221 ttcaagtatt ctgtcagacc taagatctct gctgcattgt ccacacgttt tttgatgtct
    32281 gctttatcat atttacgtaa tttcaagcca aaagccatat tatcaaaaac agtcatatga
    32341 ggatacaatg cgtagttttg gaaaaccatg gcaatatcac ggtctttagg tgccacgtca
    32401 ttcatgactt ttccgccgat atttagttct ccttcagaga tatcttccag tcccgcgatc
    32461 attcgcaaag tagttgactt tccacaaccg gaaggaccaa caaaaacgat aaattcacgg
    32521 tctttgattg atagattaaa atcagttaca gaataatttt ctgcattgtc atatttttta
    32581 tatacgtttt tcagagccat ttctaccata atacgttcca cactttcttt tttagtttta
    32641 ttaactattt aaagtataaa tgaaagcgtt aactaacttc aatgtcatgt tgaacaagaa
    32701 ttttttcttt tttttgtcaa tgtgctagtt ttaagcttta ataaatatcc ataagaaata
    32761 aaaactgata cactggagta aataagtatg ttaacaatat caaaaggagt gtctcttatg
    32821 aaaatcgctt tgatcgctca tgaccgaaaa aaacctatga tggtgaaatt agcaacagcg
    32881 tataaaacaa tattggaaaa acatgaactt tttgctaccg ggacgactgg gcagaggatc
    32941 acggaagcaa ccggcttgcc gatccatcgg tttaaatccg gaccgttagg cggggatcag
    33001 caaattggag ccatgatttc ggaggatcag ttagatttgg tgatttttct tcgagacccg
    33061 ctggctgcac agccccatga accagatgta acagcactta tccgtttatc tgatgtctat
    33121 gagattccgt tagctacgaa tatcggaaca gctgaggtgc tactaagagg actggaagcc
    33181 ggcttcctag attttcgaag aatcgtccat gaaatagaca agcatccact ttcattttga
    33241 tggataagcg tatggaaaaa taacgtttaa tgttcgccta tatgtagcta taaaagctaa
    33301 acaaactgat aatatgcgat ataatacgaa cgtattggat aatataaaca ataatattag
    33361 tttttcgtat ttgcagtaaa tgtgaaaata tgctatagtt tctgtgggaa aagatcccaa
    33421 aaataataca gagtagaaaa taaagcgtta agtgctaaga ggatgggatg ttgcctcttg
    33481 gacgaaggac gtaatgttcg cggttttatt ttcgcattcg ctgcatgata cgatgtagca
    33541 gctcttaaag gagatgctaa aaatgaagaa acaccggtta gacgcacgta tgattgctat
    33601 tatgggctta ttgattgcat tgatggtcac tttgtcccga ctggtagcaa tcgagacccc
    33661 atttattaaa atcagtgtca catttattcc acaagtgatc atgggcattc tttttggtcc
    33721 tttttggtca gggataggcg cagttttagc tgatttagta ggaatggccc tcttctcgaa
    33781 atctgcattc tttattggat ttacattgaa tgcatttatt gaaggagcaa tctacggatt
    33841 ctttttctat cgaaaagaga tcacttggaa aaatgccatt ctggctacgc ttagtgtcac
    33901 gctgatcatc aacttatttt tgacaccatt atggcttgcg ttgatgtacc atgtgccatt
    33961 gttcagctgg gtcgtttggg cgccccgcct gttaaaaaca gtaatatggt taccgattca
    34021 atcaattgcc atttattacg taggacgtag tattccttac aaaaaaattt taagaagttt
    34081 agcgatccac gctaaat
//
LOCUS       DACEQH010000027        33713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.027, whole genome shotgun sequence.
ACCESSION   DACEQH010000027 DACEQH010000000
VERSION     DACEQH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.027"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            4..816
                     /locus_tag="IW833_001362"
     CDS             4..816
                     /locus_tag="IW833_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ1307491.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IW833_001363"
     CDS             813..1613
                     /locus_tag="IW833_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ1307492.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IW833_001364"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IW833_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ1307493.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IW833_001365"
     CDS             3110..4819
                     /locus_tag="IW833_001365"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ1307494.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="IW833_001366"
     CDS             5031..9383
                     /locus_tag="IW833_001366"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ1307495.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="IW833_001367"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="IW833_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ1307496.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="IW833_001368"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="IW833_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ1307497.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="IW833_001369"
     CDS             11221..11514
                     /locus_tag="IW833_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ1307498.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="IW833_001370"
     CDS             11511..11822
                     /locus_tag="IW833_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ1307499.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="IW833_001371"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="IW833_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ1307500.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="IW833_001372"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="IW833_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ1307501.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="IW833_001373"
     CDS             complement(14623..14844)
                     /locus_tag="IW833_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1307502.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17654
                     /locus_tag="IW833_001374"
                     /pseudo
     CDS             15292..17654
                     /locus_tag="IW833_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18022..18945
                     /gene="truB"
                     /locus_tag="IW833_001375"
     CDS             18022..18945
                     /gene="truB"
                     /locus_tag="IW833_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ1307503.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18949..19890
                     /gene="ribF"
                     /locus_tag="IW833_001376"
     CDS             18949..19890
                     /gene="ribF"
                     /locus_tag="IW833_001376"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ1307504.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20242..21027
                     /locus_tag="IW833_001377"
     CDS             20242..21027
                     /locus_tag="IW833_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1307505.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21040..21834
                     /locus_tag="IW833_001378"
     CDS             21040..21834
                     /locus_tag="IW833_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ1307506.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKN"
     gene            complement(22014..22466)
                     /locus_tag="IW833_001379"
     CDS             complement(22014..22466)
                     /locus_tag="IW833_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1307507.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22931..23299)
                     /locus_tag="IW833_001380"
     CDS             complement(22931..23299)
                     /locus_tag="IW833_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ1307508.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23449..23931
                     /locus_tag="IW833_001381"
     CDS             23449..23931
                     /locus_tag="IW833_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ1307509.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            23968..24978
                     /locus_tag="IW833_001382"
     CDS             23968..24978
                     /locus_tag="IW833_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ1307510.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25093..25806)
                     /locus_tag="IW833_001383"
     CDS             complement(25093..25806)
                     /locus_tag="IW833_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307511.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(25996..26586)
                     /locus_tag="IW833_001384"
     CDS             complement(25996..26586)
                     /locus_tag="IW833_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ1307512.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27070..27297
                     /locus_tag="IW833_001385"
     CDS             27070..27297
                     /locus_tag="IW833_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ1307513.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27313..28479
                     /locus_tag="IW833_001386"
     CDS             27313..28479
                     /locus_tag="IW833_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ1307514.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28713..29756
                     /gene="hrcA"
                     /locus_tag="IW833_001387"
     CDS             28713..29756
                     /gene="hrcA"
                     /locus_tag="IW833_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ1307515.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29850..30413
                     /gene="grpE"
                     /locus_tag="IW833_001388"
     CDS             29850..30413
                     /gene="grpE"
                     /locus_tag="IW833_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ1307516.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30464..32293
                     /gene="dnaK"
                     /locus_tag="IW833_001389"
     CDS             30464..32293
                     /gene="dnaK"
                     /locus_tag="IW833_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ1307517.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32444..33610
                     /gene="dnaJ"
                     /locus_tag="IW833_001390"
     CDS             32444..33610
                     /gene="dnaJ"
                     /locus_tag="IW833_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ1307518.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT        11460 a         5813 c         7275 g         9165 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatcctg tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa acccgaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatattga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaaacgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga acctttcttt attgaaggaa atgattcaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa ttaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaatcgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt tttgcaagtt
    16741 acgaatcatt cttgcgagtg gttgactcta tgttgactca gcacttcaaa tggatgcgga
    16801 aatcacatga cctttcttgg cgaaatgatt atccttcgct aaatctcatt gcaagctcaa
    16861 cagtcttcca gcaagatcac aatggatata gccaccaaga tccaggaata ttgacacatt
    16921 tggcagaaaa gaaggcggag ttcatccgtg aatatctgcc tgcggatgca aatacattgc
    16981 tcgcagtcat ggataaagca ttccgctcgt cagaaaaaat caatttgatc atctcttcca
    17041 aacatccgcg tgctcaattt tactcagcag aagaagcagc cgtcttagtg aatgaaggct
    17101 tgaaaatcat tgattgggct tcgacagcaa aagaggagga acctgaattg gtcattgctg
    17161 ctgcaggaac ggaatctaac ttagaagcat tagcagctgt aactttgctg ctggaagaat
    17221 tcccaaaact aaaaattcgg ttcatcaatg tcgtagattt attgaaatta cgtcatccaa
    17281 gtcaagatcc gcgtgggttg agcgatgaag aatttgatca atattttaca aaagacaaac
    17341 cgatcttatt tgctttccac ggttatgaaa cattggtccg cacgattttc tttgatcgtc
    17401 ataaccatca tttaatgatt catggctaca aagaaaatgg agatatcacg acaccatttg
    17461 acatgcgtgt agtaaatgaa cttgatcgtt atcatttggc aaaagacgca gctttaaaaa
    17521 tcaaaggaag tcaagcagaa gattttgcga aaaaaatgga tcagaaatta caagaacatc
    17581 aaaactatat tcgtgaaaat ggtattgatc tgccagaagt attggattgg aaatggaaaa
    17641 atcttgacca ataaaatgat aaaatcgatc ccgcaaaata gaatttttta cattgattaa
    17701 gaaactgaaa aaataaataa aaaaaacgga aaaaagactg gaagaatggc cagtcttttt
    17761 tttcgttttt gggaagtgaa aaccaaacta tttacctgta agccacccat aaatccaatc
    17821 aaatggtgca aagaaaaaat cttccattct attccctcct ttaaaaaagc ataacatgat
    17881 ttctatttac gtgagcatga attttgttac gtttttattg ccgaaaaata aaaaccgttc
    17941 attatgatgt caaattcgtc agtaattgat gcaatgtgtt ataataattc agttaaatca
    18001 aaaatgaaat ggagaaaaga catggacggg ttattacctt tatggaaaga acgcggaatg
    18061 acaagtcatg attgcgtatt caaattaaga aaaatcttac atacgaaaaa aatcggacat
    18121 gggggtacgc ttgacccaga cgtagacggt gtgttgccca tttgtattgg aaaagccaca
    18181 aaagttatcg aatatttgac agattctggg aaaatctatg taggcgagat aactctaggt
    18241 ttcagtacaa cgacggaaga tcgatccgga gaacgggtag aaacaaaacc agtagatatg
    18301 tctttatctg aaaaaaaaat agatgaagtg atggcttcat ttattggtga gatcacccag
    18361 ataccaccaa tgtactcagc ggtaaaagtc aacggtaagc ggttatatga atatgcccgc
    18421 aataatgaaa cagtggagcg tcctgttaga aaggctcaga tttaccgttt tatccggaca
    18481 agtgaattga caagagacga agtgacagga accatttctt ggcgcttcga agtagaatgc
    18541 gggaaaggaa cctatgtccg cacattagct gtagatacag gcaagaaact tggttaccct
    18601 gcgcacatgt ctgatctgac ccgaacagca agtgccggta tgtcccagaa ggatgctctg
    18661 acattagaag aagtagctca agctgtgaaa aatgggacaa tcgatgattg tttgctgcca
    18721 atagaaaaag gagtggaacg gttcaaacga gtagatatta ctgatgaagt atggcagaaa
    18781 gtaaaaaacg gtatgagact tgattatact gtatttgcgc tcccagaaat gccgtcagaa
    18841 gaaattgctt tgttttataa aggaaaagtt gtcagtattt atcagccaaa cccgaaagaa
    18901 aaaaacaaat taaaacctag taaagtattg agaaatgagg aataacgaat gaatatcatc
    18961 aagatcagac atccttacca atcagaacaa attccagaag acgatgtcgt tttagtgctg
    19021 ggatttttcg acggcgtgca tatcggacac cagaaagtca taaagacagg tatggaaatt
    19081 gcacaaaaag aaggattgaa attagcattg atgactttca atcagcatcc atctattgtc
    19141 tttaaaaaga tcgatcctag cagcgtgaaa tatttgacta gccttaagca aaaagaagaa
    19201 aaaatggccg ctttgggtat agattattta tatgaagttg attttacttc ttcttttgct
    19261 catttagcac cgcaggaatt tgttgatcaa tacatcgtag ggttacatgc aaaatatgct
    19321 gtttccggtt ttgattatac gtatggacca aaagacattg cagatgtcgc tcattttcct
    19381 gaatatgcca aaggaagatt caaaatcgtc acagtttcta aagaagaaga acaaggtgaa
    19441 aaaatcagct ctacccgtat tcgtggatta ctagacagcg gacaagtaaa agaagcagca
    19501 aaactattag gttgcacata tgaggtagaa ggggtagttg tccatggcga tgcacgaggc
    19561 agactactag gattccctac agcaaatgtc aaagtaaaga gcacggttca tttgcctaaa
    19621 gaaggtgttt atgtgtcgga aataaaaatt ggggaaacat ggtatccagc catgggttca
    19681 attggacata atgatacatt cggtgaagga cgggagctga ctgtagagtt gtatattttg
    19741 gactttaatc aagatatcta tggtgaacat gtcgcggtac gctggaatca atttttaaga
    19801 gatcaagtga aattttctag tgtagaggaa ttgattgaac aactcaagga ggatgaacaa
    19861 gcaacagctg aatatttttc tcagcaataa aacattatgc ataaaatctc ttgtttatgt
    19921 ataactagca attacaataa aatgggtgat aaataagccc aggaaagggg ctgtgacaaa
    19981 agtcttgtca cagccttttt ttccaaataa atggtttttc agaagtagct tcttcggaaa
    20041 taagccgaaa tttcttgaag ctgaccactt ctgtcacaac ctctttcttt atagaaataa
    20101 aaggacacta gcaattcatg aaaacgattg ctagagcgta tcatttgctt tagactatat
    20161 atcttggtgt acactcactt taaggcggaa ataaaagtta tccgtaataa caggtttagg
    20221 tatgaaaaag gagtgggtac tatgtacaag gaattagaaa acaaagtcgc agtagtaact
    20281 ggtggctcaa aggggattgg aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat
    20341 gtagttgtca actataactc ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa
    20401 gaggcaggcg gaaaagcagt ctctgtacaa gcagatgtca gttctgaaga aggaatacag
    20461 cgtttacttg atacggcttt agaaaatttt gggacactgg acgtttgggt taataatgct
    20521 gggatggaaa atcagcatcc aacccatgaa cttagccttg aagattggga aagagtgtta
    20581 aaagtcaatt tgactggcgt ttttatcgga acgaaagcgg ctttaaatta ctttacagag
    20641 aataataaaa aaggaaatat catcaatatg tcttctgttc atgaacagat cccatggcca
    20701 acgtttgctc attacgctgc ttcaaaaggc ggggtaaaat tatttaccga aacggtagca
    20761 atggagtatg caccgaaggg aatcagaatc aatagtatag gccctggtgc tatccgtaca
    20821 cctattaatg cggcaaaatt tgatgatcct gaacaaaaag aactcttgga aagtatgatc
    20881 ccactaggac gcgtaggtga accaaaagaa atagcggcga cagctgcatg gttggcttca
    20941 gacgaatcca gttatgttac aggtattaca ctatttgcag atggtgggat gactttatat
    21001 ccttctttcc aaggtggaaa aggataaggg tgaataaaaa tgggtttatt gattgcattg
    21061 atcccggcag ttggctgggg gatccagcca ctgattttga aaaagattgg tggcagacca
    21121 acaaatgaga tattaggaac tggtattgga gcggtgcttg tcggcttact cgttcaatta
    21181 ttcgcttctc cagctggtat ttcagtgact acatttttga ttagtttact ttctggtgca
    21241 ttttgggtta ttggacaaac aggacagtat acatctttca acttgattgg tgtttctaaa
    21301 acgatgccaa tttcgactgc attacagtta gtcggaacct cgttgattgg tgtcttcgct
    21361 tttggagaat ggtctggaag tatggggaaa atcattggag cagcagcaat cattcttctt
    21421 gtatttggtt ctgctttaac ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca
    21481 aaaggaatta gtctattagc atcaacaagt attggttatt gggtatacag tgcattgcct
    21541 aaactagttg atgcaagcgg actttccatt ttctttccgc aaatgatcgg tgtcttttta
    21601 ggggcagtga tttatgtgat tttccgtaaa ccaaccgcat tttctgaaga caagagttgg
    21661 aaagcaacga tcgttggagc catctttagt gtgtcagcat tggcttatat tttttcagca
    21721 aatgaaaatg gggttgctac tgcatatatc attacacagc taaacgtagt tatctctaca
    21781 ttaggcggta tgtacatcct gcatgaaaat aagacaccaa aagaaaaaaa ttaacgatta
    21841 tcggattatt gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat
    21901 ggtaaaattt catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa
    21961 aaatagcgct agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat
    22021 acaagggaaa tatctgtctg gatcaataat ttttcattga tccattgact aaagtttacg
    22081 acataattga agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat
    22141 tgtaaaagac ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct
    22201 cggaattgat tgattgctgt aaaatcacga tattgaaggt tgaaacgcat cgtgattggt
    22261 gcatttagca tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg
    22321 aactcaaaaa gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt
    22381 tttccttcat gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat
    22441 tcatttactg agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact
    22501 tagttgatat ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta
    22561 aaaatcaagt cagaaaattc taacttttat ttctctaaag aaattcgttc tctcctaaag
    22621 tgatattata tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat
    22681 tttctcgtat tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt
    22741 actaagtaga aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc
    22801 ttataaaaat agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata
    22861 ggattgaaca aaacctgtat aaggtgatga atagtttcct cgccagtaaa aggtttattc
    22921 gtataaagct ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa
    22981 tcaattgttc aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat
    23041 ccgtatatac gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt
    23101 ccggtgcgat agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt
    23161 cagtaaacat aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta
    23221 tcttagcaaa aaggtctttg atcttttgtt gatgttcttt ttctttgatt tgattgatga
    23281 tcggctgaaa tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag
    23341 aaaaacaata gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat
    23401 ttatatgata agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg
    23461 gcaaagaaat tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat
    23521 acaaatgaag atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa
    23581 agcttattct taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa
    23641 caaacggatt tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca
    23701 gaacgcatgg aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa
    23761 aaacagattc cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca
    23821 ccaggtagac cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag
    23881 ctacttgaga tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa
    23941 atagtgcata cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca
    24001 acgattgggg ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa
    24061 tcagctttaa agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg
    24121 acgcaagata aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg
    24181 atgcaagatg aaaatatcca aatgatcttt ccgacttggg gaggagaatt attgattgag
    24241 attctcgatt ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat
    24301 attagtacgt tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaact
    24361 aaccttattg atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta
    24421 ctgaaaacaa gagaaggaga atcgattcta cagcaatctt ctgaaaagta tcaaaaagtg
    24481 tggccccatg agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa
    24541 tcagtcagcg gagatgcagt aaggatgaag ggacgattat taggtggttg tattgatacg
    24601 atcaggcatt taatcggtac tccttatgga gatgtccaat cttttaaaag aaagtataat
    24661 gatggaaagc cgatcttgtg gtttctagaa aactgtgagc tatcgtcagt tgatttaaga
    24721 agatcacttg ttcagatgaa actggcaggc tggtttgatg gcagctcggg gatcatgttc
    24781 ggtagaagtg cggcagagga tttgttagat tataaaatga aggatgtata cgaagatctg
    24841 tcaaaggaat taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa
    24901 ctgacattga ttaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt
    24961 ttgcaaacgt ttatttaaaa aattttttcc tgccttggag tctatcaaag accaataaat
    25021 ctaaatagaa aagaaagtga aagaagctga caaaatgctg tcagcttctt tttgtataac
    25081 atattagaaa gtctaattat ctataactgt cacatcgatt aactgtttca cgtcattggg
    25141 atttcctgta ggaatagcta ctacagatgc attatcaatg atttcttgag ttgtttctct
    25201 aatgaacgct ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata
    25261 actagtgatt gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt
    25321 cccagtgagc ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat
    25381 aggtgttcct gcaggataat gaataacatg taattcgaaa tcacccgatg tatctctaaa
    25441 agtattacct ctaatagcta gccaaacacg tgcgtactca atttgtagat cagaatatcc
    25501 tgctaacgga tctattgact gagaagactg tgaggaggtt gtgtctattg ttgaagaaga
    25561 aatatttgtc tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact
    25621 gcttgttgaa gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc
    25681 tgaactggtt tgtctgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt
    25741 actaggagac gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt
    25801 tttcatataa aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg
    25861 aatccgtaaa accttgtcta tgaatataaa tagagtgtat cttctttgat aaatgtcaga
    25921 aaaagtgtga caaataatca tcagtaaaat tacttgtcac actattgata aggagtattt
    25981 aattgaagag aatgattagg agaaaggatc tccgtacttt ttatagtaat catagaatcc
    26041 aagtttgtat agacgaatcg ttttttctct tatttcttct gtttcaaaat ggactgcttg
    26101 aagaatcaag tctgtgaatt ctataggagc tgttccattc gcagtaatca aattacgatc
    26161 tttcacagct tgttgttcta taaaatcttc tttattctga taattttccg attctaacca
    26221 aggaaattgc gcatttccgg tgtgtttgta accttgcaat aatccgctct gagctaaata
    26281 atcaactgcc ccacaaatag cagctactgg tacgtctgtt tttaacaatt tatcgattat
    26341 ttgatataaa tgatgatttt ttatgtccca tgaattgcca ccgatcataa taaaaaggtc
    26401 aactttttca ggtatttgtt cgattaagta atcgattttt gtttttaatc ctccaatgga
    26461 agtgacctct gattggatag aagcagtttt gttttgccat ttctcatttt ggtttagtgt
    26521 gctagtaata taagcacctt cccaatcggc atattcctct aatagaaacg taatcgcagt
    26581 tttcattttc aagctctcct ttactatcta taacaaggac aacagtttta acctacagac
    26641 attcattaaa attgggtagc gacagcgaaa acaacaattc ttaagtttta gacagagtat
    26701 acagatagtc aacttaatta aatacgaact aaataaaaat gacaggtaat catttttgtt
    26761 taccagcatt ataacatttt gcatacatgg atgacaaaag aaccaattcc cacttgcttg
    26821 agtacaatat taaaagaaac tggaaggcaa aaagtaatta tagaaacgaa aaataaatca
    26881 tatcgggcac tcagacatga aaacaaccct agagatatat aactaggtaa ccagtttaat
    26941 gaaagaaaaa atcatccaag aagtcgattc ttggattttt tagtggattt gttcgccaaa
    27001 tacgtttagt atgcgtatat gagaggttgt aagcaaagcg ttgtggtctg agcacttaag
    27061 gaggaatctg tgaaaatagc ttttcatatt tttgcagatt ttgacttaat gccgaaggtc
    27121 agctttgcga ttaccgttta tcgagaggct gtaaaagcac cgtgtagctc cgatgacaat
    27181 aagcaagcat ttttagagct aggtttcctt gattctaaat atgcggctta tttcaatgag
    27241 ttggtgcttg aacgcgattt taagaaggtc gaaaacaggc acgacttttc gacgtaagga
    27301 gaaagaattt caatgataga aacaataaat cccgcagaaa acaaggtttc tgcgtacatt
    27361 catatcccat tttgcgagca tatctgctat tactgtgatt ttaataaagt ttttttagaa
    27421 gggcagccag tagatgagta tgtggaaatg ctgctgaatg aaatgaaaat aatgctagaa
    27481 agtcatccga cagcgaattt agaaacactt tacgtagggg gcgggacacc aacttcttta
    27541 tctgctaaac aacttgatcg actgttatca ggtgctagag agatcctgcc ttttcaaaat
    27601 ggtaatgagt ttactgtaga agcgaatcca ggtgatttga cccgtgaaaa acttcaagta
    27661 atgaaaaact atggcgtcaa tcgtttatcc atgggagtgc agacatttga taatcgctta
    27721 ttgaaaaaaa ttggacgtaa acacaccgca gaagatgtgt atcaaactat gcaattttta
    27781 gaagcagaaa attttaccaa tgtaagcatc gatttgattt atgcattacc gggacaaaca
    27841 ctagaaggat atctggatac tttggaaaaa gctttggctt tggatctgcc tcactattct
    27901 ttgtactcat tgatcctcga aaacaaaaca atgttcatga actgggtacg acaaggaaga
    27961 ctcgaactgc ctgatcagga aacggaaacc agaatgtttg aagagacgat tgaagcaatg
    28021 gaaaagcatg gacgtcacca atatgaaata agtaactttg ggctctccgg acatgaatct
    28081 caacataacc tcatgtactg gaacaatgac cactattttg ggtttggagc tggtgctagc
    28141 ggttatcttg gagatattcg ttaccgcaac aaaggaccaa tccaacacta tttacgccca
    28201 ttacgagcaa atgaacgccc ggtattggaa gaagaaaaat tgacacgaaa aaatcaaata
    28261 gaagaagaaa tgttcttagg actaagaaaa aaaacaggga ttactaaaaa acatttctat
    28321 gatcgctatc aaaaaacaat tgaatcggtg tacggtgatg tgctaaggga attagagcaa
    28381 gaagggttac ttgttaatga agaagaccgt gtttatttga caccaagagg gacctttttg
    28441 gggaacaatg tgtttgagcg ctttttgttt gaaaaatagt ttaaagaata tttgtttttt
    28501 atctgaattt aaataaaaaa agttcaaaaa tacaataaag gactattgaa acgttgatat
    28561 aactggattt acatcaatta tttagaatta gcactcttgt gtttagagtg ctaaaaaatg
    28621 gagtctttct cttgacagga gaagggcttc ttgctatatt tataagtgga ttagcactta
    28681 gaatatatga gtgctaatga gaggtgagga agatgttaac aaagcgacaa aacgacattt
    28741 tacgtctgat catccaacac tatacgagta gtggcgtgcc cgttggttcc aaaacattga
    28801 tgtctgaggg agtcaaggcc agctcagcaa cgattcgcaa tgatatgaaa gcattagaag
    28861 atgaaggatt gctgttgaag actcactctt cttctgggcg gattccatct gctttaggct
    28921 atcgttatta cgtggatcat ttgttaaaac ctgctcgcgt agcagaagat gatttgctgc
    28981 tgattcgtca gtctctaggc agagaatttc atgaaatcaa tgagattatt aagcaatctg
    29041 ctgagatatt atctgagctg acaagctata cgactttttc attgggacct gaaataaaag
    29101 accgccgact gactggcttt cgtatcgtac ccttgaataa tcgacagatt attgctatcg
    29161 tcgtaacaga tatggggaat gtagaaagcc aagtgttcac tcttcccgaa tatatgggaa
    29221 gtcaggaact agaaaaaatg gtacagattg taaacgaccg tctagtcgga gatcctctga
    29281 tgacggtgta ccataaactt cgtacggaga ttccgatgat tcttcataaa tatttccaaa
    29341 cgacagaagg gatgtctcag ttatttgaca ccgttcttgg tcatgttttt gaagataagg
    29401 tttatgtcag tggacaaatg aatctcttgg attatgagcc ccgtcaagat cttgatcagt
    29461 tcaaatcgat gttttctttt atgaaagact cagacggact tacacaaatg atcactccga
    29521 tggatagcaa tattcatatc aagattggtg ctgaattagg caatgattta ttgcagaata
    29581 tgagtatgat tcaagcaagt tacgagatag atggtcatgg aagaggtaca atagccttgt
    29641 tagggcctac tagtatgcct tattctcgga tgttcggtct agtcgatgtt tatcgaagag
    29701 aactagcagc taaacttgct gattactacc gttcgcttga cttgtcaaac tcgtaatgtg
    29761 aataacgaag ctatttgcag ctgatcgttc tctctttctt agtaagagca atcaaacagc
    29821 tgactgaata atgaaaggaa gactgtatcg tgaaagaaaa tcaagaagaa ctagataaag
    29881 aaatgacaga tgctcagcca gaaccggaaa tcgatgtcga agctgcagaa gattcgggta
    29941 tttcagaagc tgaagcggaa gaattcgaaa cagcgaaatt aaaagctgag ttagaagaaa
    30001 tggaagataa atacttgcgg gctcgtgctg aaatcgcaaa tatggcaaat cgaggaaaaa
    30061 atgagcgtga gcaattacaa aaataccgtt cacaagattt agccaaaaaa ttacttccat
    30121 caattgacaa cctggaacgt gcattagcca cagaagtgtc agatgatcaa ggggcaggtt
    30181 tgaaaaaagg cgtggaaatg gtcttggaaa gtttgcgtaa cgcactggaa gaagaaggaa
    30241 ttgaaaaaat ccctgctaaa ggagaagcct ttgatcctaa tcttcatcaa gcagtacaaa
    30301 ctgttccagc gactgaagat acaccagccg atacgattgt agaagtacta caagaaggct
    30361 acaaattaca tgatcgcgtg ttaagaccaa ccatggtcat tgttgcacaa taattgtgcg
    30421 gatataaaac ttacactttc aattaaagaa ggagacgtta attatgagta aaattatcgg
    30481 tattgactta ggtacgacta actctgcagt agcagtattg gaaggaaacg aagcaaaaat
    30541 catcgctaac ccagaaggaa accgtacaac accttctgtt gtatcattca aaaatggaga
    30601 aatccaagta ggggaagttg caaaacgtca agctgtgaca aacccaaata cgatttcatc
    30661 aatcaaacgc catatcggtg aagcaggtta caaagtagat gtagaaggta aatcatatac
    30721 accacaagaa atctcagcaa tgatccttca atatatcaaa ggattcgcag aagactattt
    30781 aggtgaaaaa gtcgaaaaag cagttatcac tgtgccagct tactttaatg atgcacaacg
    30841 tcaagcaaca aaagatgctg gtaagatcgc tggtctagaa gtagaacgta tcgtcaatga
    30901 accaactgca gctgctcttg cctatggttt agataaaaca gatcgcgacg aaaaaatctt
    30961 agtattcgac cttggtggcg gtacttttga cgtatctatc cttgaactag gagacggtgt
    31021 ctttgatgta ttgtcaacag ctggcgacaa ccatctaggt ggggacgact ttgataacaa
    31081 aatcatcgac tacatggtag cagaattcaa aaaagaaaac agcattgact tgtctcaaga
    31141 caaaatggct ttacaacgct tgaaagacgc tgctgaaaaa gcgaaaaaag atttatctgg
    31201 cgtatcaagc acacaaatca gcttgccatt tatcacagca ggagaatctg gaccattgca
    31261 tttggaaatg acattgacac gtgcaaaatt tgatgaatta acagctgact tagtagaacg
    31321 tacaaaagta ccagtacgtc aagcgctaaa agatgcaggt ttatcacaat cagaaatcga
    31381 cgaagttatt ttagtcggtg gatctactcg tatccctgca gttgtagaag cagtacgtaa
    31441 agaaacagga aaagaaccaa acaaatcagt taaccctgat gaagtagttg ctatgggtgc
    31501 tgctatccaa ggtggcgtga tcactggtga tgtgaaagat gttgttttac ttgacgttac
    31561 accattgtca ttaggtatcg aaacaatggg tggagtgttc actaaattga ttgaccgcaa
    31621 tacaacgatt ccaacaagta aatcacaagt attctcaact gcggcagata atcaaccagc
    31681 agtagacatc catgtattac aaggtgaacg cccaatggct gctgacaaca aaactctagg
    31741 aagattccaa ttgacagata ttccagcagc tcctcgtggt attccacaaa tcgaagtaac
    31801 attcgatatt gataaaaacg ggattgtaaa tgtatctgcg aaagatctag gtacacaaaa
    31861 agaacaaaaa atcacgatca aatcttcttc aggtctaaca gacgaagaaa tcgaacgtat
    31921 ggtaaaagat gcagaagcaa atgcagaagc agataaagct cgtaaagagg aagttgacct
    31981 acgtaatgat gtagatgcat tgctattctc tgtggacaaa acattgaaag aattagaagg
    32041 caaagtggac gaagaagaag tgaaaaaagc agaaacagct cgcgatgaat tgaaagccgc
    32101 tgttgaagca aacaacatcg aagaaatgaa aactaaacgc gatgcattga acgaaatcgt
    32161 tcaaaacttg actgttaaac tatacgaaca agcggcacaa caacaagctc aagaaaaccc
    32221 agaagcagct caaggcggag cagatgatgt cgttgatgct gattttgaag aagtcgatgg
    32281 cgacgataaa taatcagttc actgaatttc gagagaaggc caaagttgct actttggctt
    32341 tttcttttga aaaatagttt gaagtatcgg tttttgtttc aaacaaaata tgatatgata
    32401 acaagcgacg tgaaaatgat gagaaccaat aggaggaaag cctatggcaa caaaacgtga
    32461 ttattatgaa gtcttagggc tctcaaaagg agcatcagaa gatgaaataa agaaagctta
    32521 tcgtaagctt tcgaaaaaat accatcctga catcaataaa gaacctgatg cggaagaaaa
    32581 attcaaagaa gtatcagaag catatgagat tttaagtgac ccgcaaaaac gcgctgcata
    32641 tgaccaatac gggcatgcag gcacagatcc aaattacggt gctggtggag ctggtggcgg
    32701 atttggtgga ttcggcggct tctctggtgg cggcttcggt ggttttgaag atatcttcga
    32761 ctctttcttt ggaggaggcg gtcggtcagt cgatccaaat gcgcctcgtc aaggtgctga
    32821 tttgcaatat acggtcgact tgtcttttga agaagcgatt tttggtgtag aaaaagaaat
    32881 caagtataat cgggaagaaa tctgtcatac ttgtggtggg aacggtgcaa aaccaggcac
    32941 acaaccaact acttgtcata aatgtcatgg tgcaggtacg attaatgtag aacgccaaac
    33001 accacttgga cgagtgatga gccgtcaaac ttgtgatgtt tgtcatggga cagggaaaga
    33061 aatcaaagaa ccttgtccaa cctgtcatgg ctcaggacat gagaagaaag cacattcagt
    33121 taaagtgaat gtaccagctg gtgtagaaga aggacaacaa atgcgtttag ctggtcaagg
    33181 agaagctgga gaaaacggcg gaccatttgg tgatttgtat gtggtcttcc gtgtagaaga
    33241 aagtgatatc tttgatcgtg aaggttctga aatttactat gaattgcctt taagttttgt
    33301 acaagcagct ttgggggatg aagtccaagt accaactgtc catggagatg tgaaattgaa
    33361 gatccctgct ggaacacaga caggtacgaa cttccgttta cgtggaaaag gcgctccgaa
    33421 gttacgtggt ggtgcaacag gcgaccaaca tgtcaaagta aaactgatca caccgaaaaa
    33481 tctaaatgaa caacaaagag aagcattacg tgcattcgct gaagctggcg gcatcaaagt
    33541 tgaagaacag caagaagacg gtttcttcga taagtttaaa gatgcttttg gcgggaaaaa
    33601 gcggaagtag tttttctagt aaaaacgcta agtcattaat aattaaaaac cagacgaaca
    33661 atcatatgtt cgcctggttt ttttgtagtt gtcatgtttg taccaacaaa aaa
//
LOCUS       DACEQH010000028        33638 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.028, whole genome shotgun sequence.
ACCESSION   DACEQH010000028 DACEQH010000000
VERSION     DACEQH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.028"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(194..451)
                     /locus_tag="IW833_001391"
     CDS             complement(194..451)
                     /locus_tag="IW833_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307519.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            614..1588
                     /locus_tag="IW833_001392"
     CDS             614..1588
                     /locus_tag="IW833_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1307520.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            2318..2635
                     /locus_tag="IW833_001393"
     CDS             2318..2635
                     /locus_tag="IW833_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307521.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(2839..3204)
                     /locus_tag="IW833_001394"
     CDS             complement(2839..3204)
                     /locus_tag="IW833_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307522.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            3412..3894
                     /locus_tag="IW833_001395"
     CDS             3412..3894
                     /locus_tag="IW833_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307523.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            4091..4981
                     /gene="pstS"
                     /locus_tag="IW833_001396"
     CDS             4091..4981
                     /gene="pstS"
                     /locus_tag="IW833_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ1307524.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            5306..7777
                     /locus_tag="IW833_001397"
     CDS             5306..7777
                     /locus_tag="IW833_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1307525.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7880..9616)
                     /locus_tag="IW833_001398"
     CDS             complement(7880..9616)
                     /locus_tag="IW833_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ1307526.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(9613..10317)
                     /locus_tag="IW833_001399"
     CDS             complement(9613..10317)
                     /locus_tag="IW833_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1307527.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            10448..10951
                     /locus_tag="IW833_001400"
     CDS             10448..10951
                     /locus_tag="IW833_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ1307528.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            10911..11249
                     /locus_tag="IW833_001401"
     CDS             10911..11249
                     /locus_tag="IW833_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ1307529.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            11270..12688
                     /gene="ffh"
                     /locus_tag="IW833_001402"
     CDS             11270..12688
                     /gene="ffh"
                     /locus_tag="IW833_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ1307530.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            12800..13378
                     /locus_tag="IW833_001403"
     CDS             12800..13378
                     /locus_tag="IW833_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ1307531.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            13382..13903
                     /locus_tag="IW833_001404"
     CDS             13382..13903
                     /locus_tag="IW833_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1307532.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(13982..14536)
                     /locus_tag="IW833_001405"
     CDS             complement(13982..14536)
                     /locus_tag="IW833_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1307533.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            14789..15064
                     /gene="rpsP"
                     /locus_tag="IW833_001406"
     CDS             14789..15064
                     /gene="rpsP"
                     /locus_tag="IW833_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ1307534.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            15076..15327
                     /locus_tag="IW833_001407"
     CDS             15076..15327
                     /locus_tag="IW833_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1307535.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            15452..16243
                     /locus_tag="IW833_001408"
     CDS             15452..16243
                     /locus_tag="IW833_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307536.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            16413..16934
                     /gene="rimM"
                     /locus_tag="IW833_001409"
     CDS             16413..16934
                     /gene="rimM"
                     /locus_tag="IW833_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ1307537.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            16924..17685
                     /gene="trmD"
                     /locus_tag="IW833_001410"
     CDS             16924..17685
                     /gene="trmD"
                     /locus_tag="IW833_001410"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ1307538.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            17821..18168
                     /gene="rplS"
                     /locus_tag="IW833_001411"
     CDS             17821..18168
                     /gene="rplS"
                     /locus_tag="IW833_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ1307539.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            18541..19203
                     /locus_tag="IW833_001412"
     CDS             18541..19203
                     /locus_tag="IW833_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307540.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            19282..22509
                     /gene="fss3"
                     /locus_tag="IW833_001413"
     CDS             19282..22509
                     /gene="fss3"
                     /locus_tag="IW833_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1307541.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            22623..22937
                     /locus_tag="IW833_001414"
     CDS             22623..22937
                     /locus_tag="IW833_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1307542.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            22950..23324
                     /locus_tag="IW833_001415"
     CDS             22950..23324
                     /locus_tag="IW833_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1307543.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            23334..23678
                     /locus_tag="IW833_001416"
     CDS             23334..23678
                     /locus_tag="IW833_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ1307544.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            23749..25101
                     /locus_tag="IW833_001417"
     CDS             23749..25101
                     /locus_tag="IW833_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1307545.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            25161..26303
                     /locus_tag="IW833_001418"
     CDS             25161..26303
                     /locus_tag="IW833_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAQ1307546.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            26328..26582
                     /locus_tag="IW833_001419"
     CDS             26328..26582
                     /locus_tag="IW833_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307547.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            26838..28022
                     /locus_tag="IW833_001420"
     CDS             26838..28022
                     /locus_tag="IW833_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1307548.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            28037..28156
                     /locus_tag="IW833_001421"
     CDS             28037..28156
                     /locus_tag="IW833_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1307549.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            28161..28382
                     /locus_tag="IW833_001422"
     CDS             28161..28382
                     /locus_tag="IW833_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307550.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            28395..28895
                     /locus_tag="IW833_001423"
     CDS             28395..28895
                     /locus_tag="IW833_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1307551.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            28992..30839
                     /locus_tag="IW833_001424"
     CDS             28992..30839
                     /locus_tag="IW833_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAQ1307552.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            30842..31738
                     /locus_tag="IW833_001425"
     CDS             30842..31738
                     /locus_tag="IW833_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1307553.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            31788..32177
                     /locus_tag="IW833_001426"
     CDS             31788..32177
                     /locus_tag="IW833_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1307554.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            32164..>33638
                     /locus_tag="IW833_001427"
     CDS             32164..>33638
                     /locus_tag="IW833_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1307555.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPLSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT        11304 a         5620 c         7014 g         9700 t
ORIGIN      
        1 ttgtacaatt aagaaaagag ataagacttt acattcctaa aaaaatgtaa agtcttatct
       61 cttttcttaa tgctataaat tcataataac attatggaca ataaatttaa aatttattca
      121 gactgtagac aaactcctaa atttagagtg ttgtttgcag tctgacgact caaaaatgag
      181 tcgtataaat caattatagt aataagtcat tgatttcttt aaattctttg tcaagttctt
      241 tttcatcata tttttcatat ttatcagctt catgaagaac ttttttaatt tcatgaatag
      301 cctttcgttc aactaaccat ttttttaggt tatgtttttt ttctggattt tcagataacg
      361 tatcaagttc gtctaactct ttatccagtt tattgacaac agaaacaatt ttgtttacaa
      421 ctttttcttc tttgttttct aattttgaca tttatcttca cgtccttttt atttgataat
      481 ttaagtatag aactttgata tagtttatac aaataaaaac gcttctttgt gtattctgtg
      541 cttttctttt ggaatgagga aaactttgga gtaatatgaa cgtggacaga aaaatattta
      601 ggaggaataa actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag
      661 gaattttgat tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa
      721 gttgaatttt cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc
      781 tggtatagct gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc
      841 tgggctaaat ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt
      901 atctcctttt gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa
      961 aaaattgtta aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc
     1021 ccctttacat tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga
     1081 tggacttcat atatatgata accctgtggg cgttcttacc aataatcctt catttgacta
     1141 tcaattattt aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt
     1201 ttcaaatcaa ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg
     1261 agatttatcc tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc
     1321 tggagattca gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca
     1381 acaaaaaggt ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg
     1441 ttgcaatgtt gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc
     1501 aattgatatg aataaagaag acttagatag tcataagtta attagttatc caattataga
     1561 aaaacaacaa attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga
     1621 tataatacaa aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc
     1681 ttttttgtaa tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc
     1741 aacgatagtg tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt
     1801 tttacataac ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt
     1861 ttaaagcgca ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt
     1921 tgaattcact aagaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt
     1981 aggatttaga gtaattaatt agatgtttat tttataggac tgatattata gattacaaat
     2041 agtatcgtat ttcgcaatct taaaattatc ttcttactat gaataagata ataaaatttg
     2101 tatttgtcct ttttaggtaa cttattttaa tctatatacc tttctggatc aacgaaagta
     2161 tactttatat agtcataacg ccgatggtaa ctatgcactg taacgataat gctgctaaaa
     2221 tatcttttta cgaaaaacat cacaaggaaa aacttaacta tggtattatc taaacataat
     2281 tttttcggta tttaatattt aattaggagg gaatattttg cttttttata gtaattttat
     2341 cttaatagta gcgatattat tacttctgaa tatatggatt tttgatagga gtagaaatgc
     2401 aagtataggt tttagaacta aacgaagtct gtcttctaaa aaaaattggg tatactcaca
     2461 aactattttt tatggaggaa ttgttttaat tagtttactt tcttcaacac tttattcttt
     2521 gaatattata gatgtttcca cctcaaattc aatatctatt attggaatca ttattgctgc
     2581 aattattaca caactgtttt tagtgtttgg agagaagaaa agaagtaaaa aatagaaaaa
     2641 ctagtgatag ttttggtgat agtgatatta aaaatcagaa aagatagaaa aagcaggaga
     2701 tagataaaat gctgatattt cagcattttt taagagtata aaagagaaaa aaagtaataa
     2761 atgtatacat gatataagaa aaacatatat gtataaaaag aagaaagtag cttctctttg
     2821 tacatatatg gtttgttttt atggatgccg agctttttta taagataagg ctaccgccca
     2881 taacgaccca aggatttgtg gaactaagta aatacctgca agaagcaaga tcagctgggt
     2941 cggttttaaa ggatgataga agtaacgcca ttcagtaaaa ttggcgcaaa ataaatagga
     3001 agtgacagta gatataatat tggcaatgaa taaagatata atacgcctgt tcctttgaaa
     3061 ataatatcca gcaaaaagag ataaaaatat caatgtgatg aacccgctta cggaatgata
     3121 acgaaaatca agcagtaagg cgaaatgcac ataaggaaca aaagataaaa gcaacggaag
     3181 gtttttccag caagttttta tcatctttca tcaccaccta acaagtgtta ttattttgtt
     3241 agttttattg taaattgaat cctgcaataa tcaattactt tttcattatt tctagattaa
     3301 atgaataaac tctcatgcca ttatctctaa aaaaaaagtt tttcccccta atttttttag
     3361 atagaaaatt gtatagtatg atgagagaaa aagtagtttg ggggaacgtt aatgagaaga
     3421 aaagcagtaa tcgatcggct ttatttattt ttttggtttt ttcctatcgt tttgctacct
     3481 tttacatggg taaatgctgt tgtacaaggt gcggagatat taggcctgat ccaaggaagc
     3541 ggatttgtgg tttctaggaa ttttccagct ttgactgttt tctatttagt gataagtgta
     3601 ggtctcttat taccaaaatc tctgatcaat cactacaatt ggcagctgct ttacttttct
     3661 tgcttattag tatttacatc tatgttttct ttgctgcctc gcttgattat cggccctaat
     3721 ggtttgagcg agagtggatt gaaggattca gcttattcct ccatgtattt cttgacgatc
     3781 aaaccatggt tctatttagt aattgcctca tttattcttg caacattttt ttacttctgg
     3841 acagaaatca ggataaaaag tgaacgatct gtatctataa aaaataacta ttgatggtgg
     3901 atcatcagta aaaaagcata tgagctgcgt aaaatgcatg tttctcatat gcttttttta
     3961 ttattatgta aagaatgcga aaaacgttta catgattttt acataactta actatatttt
     4021 tacagcaacc atacgaggga tttacagtcg actgataaac tttagttgta aataagaaag
     4081 gagcaataaa atgaaaaaag tattgtttac tctaggtatg atgggaattc ttttagccgg
     4141 atgcggctca ggatcaggaa atacagatgg ctctgccaca caaaataatt caagtgactc
     4201 aaatgaacaa gtaaaaatcg ttgcggtagg ttcgacagca ttacagccgt tagtcgatgc
     4261 agcacaagaa agctttgtac aagaaaatcc aaattatcaa atctctgtac aaggaggagg
     4321 aagcggaact ggtttgagcc aagtcgaagc tggagcagtt actatcggaa actctgatgt
     4381 atttgcagaa gaaagagacg gagttgatgc ttcaaaatta gtggatcata aagtagcagt
     4441 ggtcgggatg gcgccaatcg tcaataaaga tacagatgtc aaagatatca ccaaacagga
     4501 attgattgat atctttacag gaaaaatcac taactggaag gaagttggcg ggaaagatca
     4561 aaaaatcaac gtcgtgaacc gtgcaaacgg cagcggtacc cgcgcgacat tcgaaaaatg
     4621 gggactggat ggtgctaccc ctgtgcagtc ccaagaacaa gattcatcag gaacagttcg
     4681 gcagctggta agtcaaacac caggagcaat cagctattta gctttctcat atctcgatga
     4741 ttccacacaa gcactaagta tcgacggtgt agaaccaaaa gaagagaatg tagcagataa
     4801 cagctgggga atctggtcat atgaacatat gtacacgaac ggaaaacctt cccctgaagt
     4861 ccaaaaattc ttagactaca tgatgacaga agaaatccaa gaaggacctg tcaaagaatt
     4921 gggctattta ccaatcacaa tgatggaagt agaacgtgat cacgaaggaa atatcaaata
     4981 actagcagat aaacatgcca cggtagtcgt tactattaag gaaaaatcaa ctatgtttcc
     5041 cacatataca tttttccttt aattgtgaaa aatagttcct catattttgc cgttttttga
     5101 gctaataccg agggtcagct gattaaacac cgtttattcg aaaaaaagag ctacgacata
     5161 gaactgtcgt agctcttttt ttgctatatg acaggaatga aaggtgtttg caagcttaat
     5221 gtgaggaaca ctttccatat ctgttctata ctggatagga gaagtttgga aatatccaga
     5281 aaacgaaatc aatcgaggta gccacatgaa gaataaatgg ataaatcgaa gtaaaaaaaa
     5341 gaaagtaaca caaagatcat cagataaagt catccgtctt tttccatcgt ataaagaagg
     5401 attaacggca gatcaagtca aggaacgaat agaaaaaggt gcagcaaata attcagtcga
     5461 tcccacattc aaaacaaatc agcaaatcgt attggaaaac atatttacgt atttcaattt
     5521 gatctttctt atcttagcca ttttgctttg tttagtagaa tcctataaga acttgacttt
     5581 tttaccagtt atcatagcca atacaggaat cgccattttt caggaaattc gatccaaaaa
     5641 gatcttggac gaactaaatg ttttaaatac agcaaaagtc aatgtcatac gggacgggat
     5701 agaacaaaca atagatatag aagaccttgt aatagatgac attgttattt tagagactgg
     5761 acatcaaatt ccagcagatg cagaggtcgt ggaaggaaaa ctgcaagtta atgaggcttt
     5821 attgacaggc gaagcagatg aaataaccaa agaaataggc gatccattaa tgtccggcag
     5881 tttcattgtt tccggaaaag cttacgcccg attagacaaa gttggagcag attcgtatat
     5941 ttcccagctg acagtcaaag ccaagacaat gggagaagga gaacagtcgg agatgattgc
     6001 ttctcttaat caattgatca aatggattgg gatcatcatt attcctatcg gtatcctgct
     6061 tttttctcaa agttactttt ttaatggaaa cacgatcaaa gaaagtgtcg ttgcaatgga
     6121 agcagcattg atcggtatga tacctgaagg actgtacttg ctgacgacga ttgcattagc
     6181 attaagtgcg actaaactag caaagcaaag agttctttta cacaatatga aaagcattga
     6241 gacattggca agggtaaatg tcctttgtgt agataaaaca ggaacgatca cagaaaataa
     6301 aatgtctgtt caaaaagtga tcgtctctaa aaaacaagaa ataatcagtc agtcagatca
     6361 attggaagca tgcattgctg actatgcaaa agcgatggca ggagacaatg caacaatgga
     6421 agctgttaaa gcatatttta caaaaaccac tgatcaatcc tatcaccatg ttattccttt
     6481 ttcttctgtc caaaaattca gcagtgtagc gttggcagac aaggtgtatg ttttaggagc
     6541 gcccgaaatg gttttgcgcg atcaattgcc ggaatatgcg gaagagtttg tttctttcgc
     6601 tgaaaaaggc tatcgcgtgt tggtctttgg tgtgtacgat gggattcttg aagaaccagt
     6661 attgacagaa gcagtcacac cattaggcta tattctgatt gctaatccta tcagaaaaga
     6721 agcaagagcc acatttgatt acttcaaaaa acagcaagta gccatcaaag tgatttctgg
     6781 agataatccg ctcaccgtat ccaatgtagc tgtccaagca gggatttcac atgctgatca
     6841 gtacgttgat gcatctaaat tatcagaaga agaatatgaa gcagctgtcg aaaaatatac
     6901 agtttttgga cgcgtcaagc cagaacaaaa gaaaatcttc gttcaattga taaagcgtaa
     6961 caatacagta gctatgacag gtgatggcgt gaatgatatt ctagccatga aagaagcaga
     7021 ctgcagtata gcgatggctt ccggaaatga agccgccatg caggcctcac aagtggtctt
     7081 acttgattct gacttttcac gcatgccaga agttgttgca gaaggacggc gcgttgtgaa
     7141 caatattgag cgatcagcca gcttgttttt agtgaagaat attttctcct ttttgctctc
     7201 actgttttca gtgatctttg ctctgaccta tcctttagaa ccatcacaaa tcacattgat
     7261 tagtttgttt actatcggac ttccatcttt cttgcttgct ctagaagaaa acaaaaagcg
     7321 aatcagaggg aaattcatca tgaatgtgat ggaaaaagct gtgccaggtg gactaacaga
     7381 tatgatcgta gtcggtgcgt tggtgatctg cggggtgact ttgaatctaa ataaaacaga
     7441 tgtttctaca gcatctacca tgcttttgat tgcagtaggg ttcctcgttc tctataaaat
     7501 ctgttcaccg ctgaataaat tcagaagtca gatcattctt ttctgtgcta gcggtaactt
     7561 tttttcggtc atctttttgc ataagctgtt ttcaatcacc ggtatctcag ctgtatcact
     7621 tttgctgttg agcattctat ttttctcggc agattcgatt tttaggcatt tgaccacatt
     7681 ggtagaatat ttgtttgaac gtaaaaatga aaaagagcaa aagccgttta gtcgcgtagc
     7741 taaaaaattt ctatcgatat ttaaacgaga aaattgacga aaagtatctc aaaaaagagt
     7801 aaaaaagaac aaccactcgt gtatctttcg aagcaggatg agtcatccaa ctttaaaacg
     7861 cacgagcagt tgctcttttt tacattttcg gaatggtaac ggtgaaagtc gaacctgttc
     7921 caagatgact gtctacagta atcgtaccat taaggagttg cacgtagtct tttacaatcg
     7981 ctaaccctaa tccggtcccg cctgaatgtc ggcttcgcgc cttgtctacg cggtaaaaac
     8041 gctcaaagat ccgttcctga tcctcaatgt cgatacctat accaaagtct tggacagaaa
     8101 acgatagatc atctgttgcc tgatagcgga taataatctc gctgtcgctt tttgaatatt
     8161 ggacagcatt ttcaatcaga tttttgataa ttggataaag gatatctgtt ttagtagtaa
     8221 atggttcgtt ctttccaatc aatcgaatcg tcaaccgttt cttttcgatg attggctgat
     8281 aattttgaac gatttgctgg aaataaggtt ctgtataaat tgtctggatc tcgtaagaat
     8341 tctcgccatc ttttgacagc tggatgattt cccgaatcag tttatctaac cggatcgcat
     8401 ctttttgcat gatctcaaga aaagaagtca gagtttgcgg gtcatctttt gcaccatcca
     8461 gtaaagtctc ggtaaaacca atcagtgaag tcacaggagt ttttaattcg tgagagacgt
     8521 tgccaacgaa atccttttgt agcttttcta aacgtcgcac tttcgttaag tcatacgcca
     8581 cccctagaat ctgtccggta ccttctgaat tagtaaagta gcgcaaactc aagtctaatg
     8641 tctgccgacc ttctgtaagg gtgatctcct gatggatcag cggatgctca ggagtcactt
     8701 gatgaatcag ctgaatgagt tttggctctt gaatgacttc tgtgtaccgc tgttcaggtt
     8761 gatagcttac cacgtttaaa tgtagctgca tctttggatt aagcagtagt aaacgagaat
     8821 cagcatcatc aatcaggaat acgccaatca tcagttcgtt cagtaacgta tataattgct
     8881 cttctgtggt agtacacgcc cgatacatct tactcatttg ttcgctcaac gcattaatcg
     8941 tctgatacaa ttcttcccat tgatcgggtg tttgcatgat tacttccgcc tgattaggat
     9001 tttgcaccat tttttttagt acgggcagca cggttttcaa tggctggttt ttttgataga
     9061 tcagataata gatcattcct gcaagaacaa ggaagaaaga cagaaagaag aaaaagaccc
     9121 aacggcgaaa actctcagtt ctcggtaaaa aaccgcttgt cggttctgcg atacgtaaaa
     9181 tggcttctaa ctggccactt tttttgactg gtaatgcgac atataaaaga tcctcattca
     9241 aagtagtact tttgcgcaaa gcgctaccta ggttcccacc attcaatacg gctttgattt
     9301 cgggacggga gtttcgctgt tcattcaatg tttcattgtt tgtgtcaaat aagatctttc
     9361 cagtagcatc cattaaagta atccgttcgt tcgactgatg gacgaattct tccaaagcag
     9421 ttttgttttg cgctgcttgg aggttttcga catctaattg ccggatcagc agttgtgctt
     9481 tttttgtcag gtagctttct tgctgttcca cgatctgctg ttgaaaataa tgggaaatca
     9541 attgccagcc accaaaaaac aaactaacta aaagaataag aaagaagaga cgttcataaa
     9601 tttttttcca tttcatcgtt ttggttcctg aaacttatag ccaaatcctc taatagtcac
     9661 caagtacttt ggatgttttg gatctcgctc gattttttct cgcaaatgac taacgtgtac
     9721 atctacgatt cggctttgtc ctgcgaagtc aaaattccaa atgcggtcta gcaatgtgtc
     9781 tcggtcgatc acacggtctt ttcgtttcat gaaataaaca agtaattcaa attctttcgg
     9841 tgttagttca atcagatggt cgtctacggt cacttgataa ttggtcaaat cagcagtgat
     9901 ttgtccaatc gatagatatt caggctcagc ttcatctttt gattgcttac gaggttcaat
     9961 ccgtctgaat attgctttca tacgggctaa tacttcgcga ggactaaatg gtttggtcaa
    10021 atagtcgtca gcacctattt ctaggccaat gatccgatcc acttggtcat ctttagccgt
    10081 cagcatcaaa atcggtgttt catttttttc ttgacgcagc cgctgagtga tctccatgcc
    10141 atccatcgtt ggaagcatca catctaaaat gatgaaatca aatgattgtt ctaatgctaa
    10201 ttctaatcct tcgcctccat ctgtggcact ggttaccttg tagccttctt tttccaaatt
    10261 gaacgtcaag agcgttacaa tagaaggttc atcatcaaca actagtattt ttttcattca
    10321 aatctccttt aaataggttt cattctgttt atattttagc atagaatcag tacaaagggc
    10381 tattttacaa aagaaataaa tggtataata ggaaggcact ttgctgaata gaaaggaaga
    10441 gcagtcaatg attggaatca gtgcatgttt aggcggtctg gcctgtcgct atgatggaca
    10501 gaaaaaagaa gtaccagaat tgatggaact agttgaaact ggagaagctg tcatggtctg
    10561 tccagaagtg atcggcggat taccaattcc tagagatccc gctgaaatca ttggtggaaa
    10621 tggattcgat gtgtggaaag atcaagcaaa agtatggacg acaagcggcg aagatgtaac
    10681 ggaagcgtac aaaaatggcg cgatcaaagc ctatcaaaaa ttgcaggaaa aacacattga
    10741 tctagtcatc atgaaggaaa aaagcccttc atgcggaacc cattcgattt atgacggaag
    10801 tttttccgga ataaaaaaaa atggtgccgg agtggctact gcttatttta ttcagcaaaa
    10861 aatgactgtg ctctctgatg aagaatggct gaaaactaga ggtgagcaga atggaaatcg
    10921 aaaagaccaa tcgaatgaac gcgctctttg aattttattc gaccttattg acagaaaaac
    10981 aaatgaacta catggaatta tattatgcag atgatttttc attaggtgag atcgcggaag
    11041 aatacgaagt aagccgacaa gcagtatatg acaatatcaa gcggacaagc aagatcttgg
    11101 aggattatga aaaaaaactc catctttttt ctaattatat tgttcgagaa caggtattgg
    11161 aaaatatgac tgcatatatt acagataagt acccggaaga taaaaaacta cttgaatatg
    11221 tacagcaaat tcaagcaatc gaagaataaa taaaaggaat gaagaaaata tggcatttga
    11281 aagcttaact gaacgtctcc agcaggcgat gggaaaaata agaaaaaaag gaaaagtatc
    11341 agaagctgac gtaaaagaaa tgatgcggga aatccgcctt gccttactag aagccgatgt
    11401 taacttgcaa gtagtcaaag attttactaa gcgcgtacgc gaacgtgcgg taggggcaga
    11461 agtgctggaa agtttatcac ctgcacaaca aatcgtaaaa atcgttgatg aagaattgac
    11521 gatcacactt ggttctgaaa cagcagagtt aaataaatca ccaaaaatac caactgttat
    11581 catgatggct ggtcttcaag gggcaggtaa aacgaccttt actggtaaat tagccaatta
    11641 tttaaagaaa aatgaaaatg ctcgtccgct cctaatcgca ggggacgtat atcgaccagc
    11701 tgcgatcgac caattgaaag tattaggtca acagctagac gttcctgtat ttgatatggg
    11761 aactgaagtc agcccggtag agatcgtccg tcaagggatg gagttagcaa aagaaaagaa
    11821 aaacgactat gtattgatcg atacggcagg acgtctgcat atcgatgaaa cattgatgga
    11881 tgagttgaaa caaatcaaag aattgacgca accaaatgag attttgcttg ttgtcgatgc
    11941 catgactggt caagatgccg tcaacgtagc tgatagcttc aaccagcaat taggtattac
    12001 aggggtcgtc atcactaaac ttgatgggga tactcgaggc ggggcagcac tatcgatccg
    12061 ctcagtgaca ggcgcaccaa tcaaattcat cggttctggt gaaaaattga ctgacttaga
    12121 agtattccac ccagatcgta tggctagccg tatccttggt atgggggata tgctgacatt
    12181 gattgaaaaa gcgcagcaag actacgacga gaaaaaagcg gaagaacttg cacagaaaat
    12241 gcgggaaaat tcattcgatt tcaatgactt tattgaacag cttgatcaag tgatgggtat
    12301 gggcccactg gaagatctaa tcaaaatgat cccaggaatg aatcaagtcc ctggaatcga
    12361 aaacgtcaag gtagatccaa aagatgttga acgcaaaaaa gcgatggttt attctatgac
    12421 gccagcagaa agagcaaatc ctgatctgtt gaacccaagc cgtcgtcgcc gaattgctgc
    12481 cggttctgga aacagtgttg ttgaagtgaa tcgaatgatc aaacaattca aggaatcaag
    12541 aaaaatgatg cagcaaatgt ccaaagggga tatgaacatc ccaggtatgg accaaatgtt
    12601 tggttcaggc gtcaaaggaa aactaggcaa aatggcgatg aatcgaatga tcaagaaaaa
    12661 taagaagaaa aagaaaaaac ggaaataaga tgaaaaaaac agcttcggta cagactgaaa
    12721 atgtctactg ccggagcttt tctatcagca atttttattc tttactaata caattactct
    12781 aaacgaagag gtggcacaga tgagatgtcc gtggagtgaa caaacagaaa gtatgcggca
    12841 atatcatgat actgtctggg gtgttcctga atatgatgac caaaagctgt tccgaaaatt
    12901 gatgcttgat attaatcaag ccggattgag ctggcagacg attttaaata aaagtgaagc
    12961 attcgatgaa gcatatgatc gattcgatat caaaaaagtc gcaaattatg gagaaacaaa
    13021 aatcagtgaa ctaatggaaa acaaaggaat tattcgtaac cgaagaaaaa tcgaagcagc
    13081 cgtccaaaac gccaaagcag tcttgaaaat tcaagaagaa atcggcagtt tttcagagta
    13141 tctttggtcg ttttctaatg gaaaaatcgt tgattcttct tatcattcac aagaagaagt
    13201 cccaacaaca agtcctcttt cagatgaatt agcaaaagat ctaaagaaaa gaggatttaa
    13261 atttatcgga agcacaacaa tctatgcttt tttagaagct gtcgggatca tcaatgatca
    13321 ccttgacagt tgtttccgca aagaagaggt cgtacgtatc ggcagaaagg gcgaataaag
    13381 catgcgaaaa atcatatttt atggtgctat aagtttagat ggctatttgg caacgaaaac
    13441 ggatgatctt caatggcttt ttgacactcc aacaggtaag caaactactt atgaagcttt
    13501 ttatgaaaca atcgatacga ccatcatggg cagaaaaact tatcaggaag caaaaaaata
    13561 tttggagacc gaagcaattt atccagagaa aaagaatttc gttttttcca cccacgcaca
    13621 attacaattg ccagatgctg aggtcgtaaa cgaagatcct gttgcttttc taaaaaatct
    13681 acaaatgaca gaaggaaaag atatctgggt tgtgggtggc ggtcgattga tcaaaccgct
    13741 attggaacat caaatgattg atgaatggta tatccagatt acccctgtat tattagggga
    13801 cgggattcct ttattccaaa aaggaacata tgaagcgcgt tttgaaatgc tagacacgac
    13861 acgattcggt gaatttatcg aattgcatta tgtacgaaag tagaagtaaa taaaataaaa
    13921 aaggcaacta tatgcagaaa tgtcctgtac atagttgcct tttttattac cgttatgctt
    13981 tttactctgt taatttgtta gaaaaatcta gatacgcatc taaaatcgtt tggaagaaac
    14041 ttaatgttcc ttcaacgata ttaccatttt catcaattag attcgcaacg tttccgatat
    14101 atgcttccgg ttgctgcaag gttggcacat tcaaaaagac aagtgattgg cgtagatggt
    14161 ggtttgcgcc aaagccgccg atcccacctg gtgatgcagt aacgactaaa cctggtttat
    14221 gatcccaaac actgtgtcca taaggtcttg agcctacatc taaagcattt ttcaatactg
    14281 ctggcacaga acggttgtat tcaggagtaa cgaaaacgac accgtctagt cccctcactt
    14341 cctcacgaaa acgagtccat tcagcaggag cctgttctgg agtttcgaga tcctcattgt
    14401 aaaatggcag atcatcgatt tttacaaata cactttcgta cccttcagga agcaatttcg
    14461 caaaagcttc tgctactttt ttgttgtaag aatcttttct taaactacca ataaaaaatc
    14521 caattttttt tgtcatacga tttttcctcc ttcaaactat ctataataat cttacaaaag
    14581 ataagtaact ggagcaaata aaaacactta ctgacaaaag ctacatagaa gatagattac
    14641 ttatttttaa caaaaaagaa gtctaaaatc ttctattttc taaaaaaaat cgaatttaat
    14701 caagctgtaa agaaaaaaag ctttacaggg taaaaaaaaa ctgttacact acatcttgtg
    14761 aataaaacaa tggaggtgga aataaataat ggcagttaaa atccgtttaa aacgtatggg
    14821 ttctaaaaag agtccgtttt accgtatcgt cgtagctgat tcacgttctc ctcgtgatgg
    14881 acgtttcatc gaaactgtag gtacttacaa tcctttgaaa gaccctgcag aagtagtttt
    14941 aaaagaagac ttagttcttg actggttatc aaaaggtgca caaccttctg acactgtacg
    15001 taacatcctt tcaaaagaag gcgttatgca aaaacaccac gaagctaaat tctcaaagaa
    15061 ataaggtgac cggatatgaa agatttaagc gacttagtct taacaatcgt tcgtccgcta
    15121 gtcacttatc ctgatcaagt caagttagac gtcgtagagt cgaaagactt ttacgagtac
    15181 aatctaacag tggctcctga agatattggc cgtatcattg gaaaacaagg gcgtgttgct
    15241 aaagcaatcc gcacaatcgt ttacggtgtt cggatcaatg caccgaaaaa agtgcgtttg
    15301 aatatcatcg atgataagga aaagtaaata aacgtgctgt actcgaattt tcgagtgcag
    15361 cttttttgtt ttttttatta attgaagaaa tgattatttg gcaaaacaag caagaaagcg
    15421 tatccttata ggaggaggga gtgaacagcc aatgagaaag aaaatgttat ttattccatt
    15481 tgctctattg ttaagcgctt gtagttcggt tactcaagaa tcagacaata cacatctatc
    15541 aagcagtata tctgaaactg ttaccagtcc agaaaaaagt agtaccaaaa cggcttccac
    15601 cactgaacaa acaatggcta cctcaaataa tgagaaaaag actgctttag accagttgaa
    15661 agaacaacag ccaaatgttc ccatgcctct agatgtacca gtctcttcag gctatctcaa
    15721 tatcgcagcg acacatacaa agcaaggtta ttctatttta tattacagaa cagatcgtcc
    15781 cctcgggttg aatgctgacg aattgaatca agaaacgcct attgcaacct atctatatca
    15841 atatgggttt gcttctagtc aagaaacaat ccaagttttg cagccatttg agatcgacac
    15901 aaatggtcag caagtggact taggttctcg aatcacaggg tatcagcaag gtgcagcagg
    15961 ttcaagtttt cttgaatggc aagaaggaaa ctggcgtatt cgaatccgtg ggaataatat
    16021 agaaggccaa gatcccttac tattagctaa agaaatcgta gcttatttgg aagaaaacag
    16081 tctaccagca cctgaacaat ttggtaaaat tactgttgat atgggtgata cgacgaatcg
    16141 agccgtagaa gtaagctggc aagaaccgaa aaatgcctat acgatcactc atcaagatcc
    16201 tatgtctgcg ttgaagatgg cagtttctat gaagaggttg tgaaatcagc gcgctgatct
    16261 gagcccatca tttaactcca agtcacagga agtaatcaaa aatagcacgc cgcatttttg
    16321 cataattttg gcttaatacc ttaaaagaaa actagaattg cctcttcaca catgctaaaa
    16381 taaaacagac aaaaaagaaa tggggatata agttgactga atacttgaat gtaggaaaaa
    16441 tcgtaaacac acaaggtatc aaaggcgaag tccgtgttat ctcaacaacg gatttcccag
    16501 aagaacgtta caaaaaagga acggtgctaa cactattcca agacggaaaa gcacctgtgg
    16561 aattaacagt caaaagtcat cgcaaacata aaaattttga cttattgagt tttgaaggac
    16621 atccttctat caatgatgtg gaaaaatatc gggatggcat attaaaagtg tcaaaagaca
    16681 actctgtaga tctagctgaa aatgaattct attaccatga gatcatcggt ctgcatgtat
    16741 tagaagatgg aactgagtta ggtaaagtaa aagaaatctt gtcaccaggt gccaatgatg
    16801 tttgggtcgt tcaacgtccg aaaaaagctg atatattgct tccatatatc gcttctgtcg
    16861 tcaaagaagt cgatttagaa gctggagtgg tccgtgtaga aattccagaa ggactgattg
    16921 acgatgaaga ttgatgtatt gactttattt ccaagaatgt ttgaaggtcc tatgggggaa
    16981 tccatcatcg gtaaggctgt agataaagga ttattagata tcaatatctc taacttccgc
    17041 gattattcag ataacaaaca tcagacagta gatgattatc catacggcgg tggagcaggt
    17101 atgctgctca aagtccagcc catctatgat aacatccaag ccattgaaaa agctgcaccg
    17161 gatgtcaaaa aaagagtaat tcttcttgac ccagctggta aacgattcga tcagaaaatg
    17221 gcagaagaat tttcacaaga agaacactta gtattcattt gtggtcatta tgagggatat
    17281 gatgaacgta tccgcacatt agtgacagat gaagtttcct taggagatta tgtccttact
    17341 gggggagaat taggtgcgat ggtcatgatc gatgcaacag ttcggctttt gccagatgtg
    17401 ttagggaatc agacttcagc tcaaacggat tcttattcaa ccggtttatt ggaacatccc
    17461 caatatactc gcccggcaga atataaaggg atgaaagtac cagaagtatt gacaaatgga
    17521 aatcataaat tgattgaaga atggcaatta aaagaatcac taagaaggac ctatcaacgt
    17581 cggcctgatc ttttggaaac acttgaatgg acacctcaaa tgacgaaatt tttagaagaa
    17641 atcaagaaag aagaacaaga aaaaacagca gatgaagcca agtgactttt tctaaaatct
    17701 tctttacagt aggttgataa tatgttagta ttttatggtg agtgcaaaag cacttaacta
    17761 ttacgatatt ccgctgtgag aaaagctcat aagaatattt ggaataagga gaagaaaacc
    17821 atgaatccat taatcgaaga attaacaaaa gaacaattac gttctgacat cccagcgttc
    17881 cgccctggtg acactgtgcg tgttcacgca aaagttgttg aaggtactcg tgaacgtatc
    17941 cagttatttg aaggtgttgt tatcaaacgc cgcggtgctg gaatcagcga aacttataca
    18001 gtacgtaaag tttctaacgg agtgggcgtt gaacgtacat tcccattgca tacaccacgt
    18061 gttgcaaaaa tcgaagtagt tcgctacggt aaagtacgtc gtgcaaaatt gtactaccta
    18121 cgtgcattac acggaaaagc tgctcgtatc aaagaaatca gaagataaac acggaaaact
    18181 tccttggaaa tctgaaattt gtgaaaagtc cttgtatatc aagggctttt tcttttttct
    18241 ctatcacagt tttaagccat ttttggctgt tttggaccat ttttcaatca tattccctta
    18301 aaaattgtcc tagattgtcc gacattgact atcagtgtcg tcaaaatagg gcaaaattta
    18361 gtagtcagct tgacgacaaa tataatacga aaaataccaa aacatttacc gaatttagca
    18421 acttttgaac ccaagtttct caaagggtca gctattagca aaaaagacaa aacatgtact
    18481 tttctactaa aaagttgatg tgataggctt ctatctaaat aactattagg agtttttatc
    18541 gtgaagcaaa atgatggacg tatcatttgg aaaaatcaaa gtgagttaaa actgatttta
    18601 acaattaatg aattcatcga aaaacacgga attacatcta gccgtcaata ccagaaaaag
    18661 ctatcagaaa atccaaattc tgcaccaagt atgtggttca ttaacaaaaa atatggatca
    18721 tgggagaatt tattgattag tataggacga gaaaatactg ggtatggaaa atgggctaga
    18781 atgtcagaac aagaattatt ggaaatagta gaagccttta ttaagtgcga gaagataacc
    18841 tctcaacgaa tgtacgaaca aaaatcagta gggaagaata ttccttcact tagtaccatt
    18901 aaaaagatgt taggagatat acgtccactt tttaaagaaa aaaatgatgg atctcgattt
    18961 actgatttcg aactgctgtt agaactaaaa aatgaaataa tccgattaaa actacaggat
    19021 gatttatcaa tgacaaagtt tcgaaaacta gttcaatccc caaaactacc atcagttgat
    19081 acaattatga aaagaacgaa caaaaattgg gaggaattga tggcagagat tggatttgat
    19141 tatcgaagaa tcaaaattta taagcagaga aacaacctat ccaagacaaa gaaaactaaa
    19201 tagtatcact ctaaatcaga cgttcaaaat taaaatttgg acgtcttttt tattacccaa
    19261 aaacgaaagg agatttattt tatgaaaaag ccaagattta agaattggcg tttgctcgca
    19321 acactgagtc tattatgcca aacaattggt ggatcgcttg gcccaacgat tgcctttgct
    19381 gatgaaatta ctcatccaca aacggtaact gtggaattgg atttggctca ccagtatgct
    19441 gtcgaaggta catttagcga tggtcgtccc atgtcagaag ttaccgttcc gcactatgca
    19501 gtttacaatg gtgtgaagca agatattttc tgtattgagc caggggtgcc gatttacaat
    19561 gagttcactc ctggttatga gaaaaatcca ttgcctgata tgtcggagaa agcgaaatta
    19621 gtttccgttt tatggaagga agctggtacg gatgtggata cacatattgt ggcccagaag
    19681 atgatttggc aagaagttaa tggctacacg cttcactcaa ttaaacgttc tgatggtagt
    19741 gcagtaaata tcgcagccat tgagtcaaaa atcaatcaag ccattgcgga ttatcaaaag
    19801 aaaccaagct tccataactc cacagtgaaa accgtgttag gtcaatcgac tactgtgaca
    19861 gatacgaacg gcttgaattt atcggagttt gatgaagttg tcgagaatat cgcaaatatt
    19921 gattatcgtg tgaacggcaa tcaactagtg attactccaa atgccaattc aaaagagagt
    19981 ggtgaattaa ccctaaagaa gtcagctaat acaggaacac cggtagctta taaaatggct
    20041 ggtcaacaaa ctttgatggc gggggcaatt gataagccca atacctacac aatcaaaatt
    20101 gatgtggaaa ctgagggttc tttgaagatt aaaaaagtcg ataaagaaac aggaaatgtt
    20161 gtaccgggaa ccgttttcca tttagacttt ggaaaaacat tacctgcaaa agacgtgacc
    20221 accgataaag aaggcattgc gacattggat gagattcctc atggcgcgaa agtaaccatt
    20281 actgaaaaat cagtgccagc tccttatacg attgacacta caccaatcac cgcaaccatt
    20341 aaatctggtg agactatttc tgtgacttca aaaaatactc gcgaaaaagg tcaaatcatt
    20401 ttgaataaaa tgggggtgga aacaggaact gatctttgga acgacaaata ctcattagcc
    20461 ggaaatacgt ttgccattcg caaagacagt ccaaccggtg aaattgtcca agaaatgacc
    20521 acggatgaaa aaggtcatgc ggaaacacca aaagagattg ccaatgcgtt ggaattggga
    20581 acctattatg tgaccgaaac caaagctagt aatggctttg tgaatacctt taaaccagta
    20641 aaagtcgaat tgaactatgt caatcaaacc gtggctcttg ttaccagtaa cgtaaaaggg
    20701 caaaaccaag aaatcactgg tgaaaccact ttgacaaaag aagacaaaga taccggtaat
    20761 gaaactcaag gtaaagctga gtttaaagga gctgaatata ctctctttac tgcaaaagat
    20821 ggtcaagcgg tcaaatggag tgaggctttt aaaccagagt tagtgaaggg aacgaaagct
    20881 tctgatgaaa cggtgacttt gactttagat gaaaagaatc aagttgctgt taaacactta
    20941 gcgattaatg agtattactg gcaagaaacc aaagcacctg aaggatatac cttggatgaa
    21001 acaaagtacc ctgtttccat caaaaaggtg gatgataacg aaaaaaatgc cgtgattacc
    21061 cgagatgtta cggcaaaaga acaagtcatt cgctttggct ttgatttctt taaatttgct
    21121 ggatcagctg ctgggactgc cgaaactggc ttcaatgatc tgacctttaa agtatcgcca
    21181 ttggaaggaa ccagtgagat tacagatgcc gaagatgaag caattacagc ttataacgag
    21241 caactaggtt ttgatggcta tggcaagttt gaaaatcttc cttatgggga ttatttactt
    21301 gaagaagtag aggctccaga aggatttcaa aaaattacgc cattagaaat ccgttctact
    21361 tttaaagaaa ataaagcgga ttacactaag agtgaataca tttttacgat taccgaaaaa
    21421 ggacaaaaac aaccaattaa aacggtgacc gttccttacg agaaactgac gaataaagca
    21481 ttttctgtta gcttgaatcg tttgatgctc tatgatttac ctgaagaaga agatagctta
    21541 acttctcttg ggacttggaa agacggaggc aaagaattga cagaccttga ttctaccgag
    21601 ctagttgata aattgagcta taacttgcat gaaatcaaag aagactggta tgtggtagcg
    21661 caagccattg atgtggaagc gacaaaagct gcccaagaaa aagatgaaaa agccaaacca
    21721 gtggtaattg ccgaaacaac cgcaacgttg gcgaataaag agaaaacagg aacttggaaa
    21781 attttgcata aattaaccgc agaacaagtt ttggataaaa ccatcgtctt attcaactat
    21841 gtgtatgaaa acaagaaacc ctatgaagca ggtgatgatc cagtagcgaa agatgctagc
    21901 ttgaacaatc aagcccaaac tgtcaattgt acggtggaac gccatgtttc catccaaaca
    21961 aaagcccacc tagaagatgg ttcgcaaacc tttactcatg gggatgtggt ggatatgttc
    22021 gatgatgtgt cgattaccca tgatgtactg gatggctcaa aagaagcttt tgaaacaatt
    22081 ctgtatgcat tacttccgga taatacgacc aaagaaattt ggaaatctgg caaaattgat
    22141 tatgaagtga atgacaaaga attcactaaa accgtacttg cggaaaaagt ggacacaagt
    22201 aaatatccag aaggtacaag ttttaccttc aaagaaatta actatggcaa agacggaaac
    22261 atcaatggga aacacaatga ggatctaaaa gaaaaaactc aaactttaac acctaaaaaa
    22321 gttccgacaa caccaagtgc accagaacaa ccggaaatac caactgttcc aagtgactct
    22381 caagaatcta gtcctacagt aaagacattc ccacaaacag gggagaaaaa ttctaatgtt
    22441 ctgctgttta ttgggtttac cttgatcttt gcgactgctg ggtattattt ctggaatcgc
    22501 cgaaactaag gtgatgtgat gaaacaacta aataggaaaa accaacgtgc gcgaccgcct
    22561 cctaaaaaaa gtggtcgcgc tatttttgta tccaaaacta aaaatgaaaa gaggaaaaga
    22621 agatggaatt gaaatttgtt gtgccagata tggccgaaac ctttggaaag ataaggtatg
    22681 ccggcgaagg cgaagtttta actgaaggat atgggcggaa tactacagta attggtcgta
    22741 gttatcattt gtattccagt aagcaacgag ccgatgatat tgaagtggtg gtagctgcgg
    22801 aagccggcga aaaggatttt gatcaagatc aaccactaaa agccgtgaat ccccatttgg
    22861 ttgccaaagg ctatgagatt gagaatcgtg ggtttaccga ctacgtgttg tatgtcgatg
    22921 atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt gttgtagatt
    22981 caggactaac gtttgggaaa ttacgatttt ccgcattacg ccgagaagta cggaagcaaa
    23041 atgaggatgg aacggttagt aacgaggtaa aagaacggac ttataatttg aaatcctctg
    23101 cgcaaggtcg aatgatccaa gttagtatcc cagcaaatgt acccttacgc gagtttgcct
    23161 atgacgcaga ggtagagttg gtcaatccaa tcatggatac ggtcgccaat tatgttttcc
    23221 gagaaggaac tactgtcaat tggttcatta aggccgatga tttagtcttg aaacgacaac
    23281 cagatcaggg aaattctaca aaccaaaatg aaggaaagaa ataggtgaag cgtatggaaa
    23341 ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggtgag ctaacggagg
    23401 aacaactcac taaagcatta acctatgatg aaaaaatgtt tctgattatg catcaagggt
    23461 tactggaagt aggtaacgat gccgaagaag attttgatgt actgaattgg ttaggggaag
    23521 aagagtcctt ctttatggtt atcgaagtaa atgaaagtct atgtcgagaa gccgaaaccg
    23581 tattggaaga aattggtgtg gaaatgccgg atgccattga agttttttta aagcaactag
    23641 tggaaacgaa acaactccct atagcagtaa atgactagca taactgcttg aaattggatc
    23701 aatgaaatcc tatgaagctt ttttcgattg gaggtggaaa ccataaaaat gtacaaaggg
    23761 catcgtatac gggcaggaga tcagcacttg gtttatcact ttgttcttgg ctggctactc
    23821 gctctgttca tcggttggat gagcgtcttt tattttcaag agcttagaca atttgatatt
    23881 tctaagctat cactttctac tatagaaatc gttcgttcta taaaagattt aatctatttg
    23941 ctaggaagtc tcgttctttc aggaagcacg atgttgcttt atatacactt ttttcaggat
    24001 cattggcgca gtttgtggca tcgacaaaag ctagcccgga tgattctaga aaatcattgg
    24061 tacgaagtaa aacaaactca gagtgaagga tttttcaaag acctcaatag tagtcgaacc
    24121 aaagagacaa tcagttactt ccccaaaatc tattaccgta tgaaggatgg tttactttct
    24181 attcgcgttc aaatttcact gggaaaatat caagatcagc ttttaaagct ggaaaagaag
    24241 ttagaaagtg gtctatattg tgaactagta gaaaaggaac tcaaagactc ctatgtggag
    24301 tacactttac tgtatgatat aattgccaat cgaattggga tagacgaagt agtggcagaa
    24361 agtggggctt tgcgattgat gaaaaatcaa gtatgggctt atgattcttt acctcatatg
    24421 ttaatcgctg gcggcacagg tggtgggaag acttactttt tactcactat catcgaggct
    24481 ttattaaaat ctgatgccga gctttttgtt cttgatccca aaaatgcgga cttagccgat
    24541 ttaggtacgg taatgcctca tgtctattcc caaaaggagg aaatttctgc ttgtgtggaa
    24601 gatttctatg agcgcatgat gactcgtagc aaggcaatga aagaaatgcc caactacaaa
    24661 acaggagaga attatgcgtt tcttggactt ccaccaaact ttttaatctt tgatgaatac
    24721 gtggtttata tggaaatgtt aacgacgaag gaaagtgcgg tgattttgaa taagctcaaa
    24781 caaatcgtga tgttaggtcg tcaatctggt ttctttctga ttctagcttg tcaaagaccg
    24841 gatgcgaaat atttaggaga cgggattcgc gatcaattta atttctgggt ggctttgggt
    24901 cgtatgagtg aactgggata ttccatgatg tttggcgagg ttgataaaaa attctttatg
    24961 aagcgtatca aaggtcgagg ctatgtagat acgggaggga gtgtgattag tgagttttat
    25021 acgccacttg taccaaaagg atatgatttc ttaagggaaa ttagcgacat tggtggatta
    25081 aacgttaata cggaaggata aaataatagt atgtagccta ggagtgtcaa gaatcctggg
    25141 ctttttttac gaggtgattt atgacatttt tagatttatt cgctgggatt ggtggctttt
    25201 gcttaggtat ggaacaagcg ggtcaccagt gtatcggctt ttgtgaaatt gatgattttg
    25261 ctcgccagag ttataaggca atccatgata caagtaagga ggtggaaatg catgacatca
    25321 caagtgtatc agacgagttt attcaatctc tcggaccagt ggatatcctt tgcggtggat
    25381 ttccgtgcca agctttttca attgcgggaa agcggcaagg attttccgat actcgaggta
    25441 ctttattctt tgaaatcgct cggttcgccg ctattctcca acctaagttt ttattccttg
    25501 agaacgtccg gggattactc aatcacgaag gaggggctac gttcgagacg atcctccgaa
    25561 cgctggatgg attggggtat gatgtggaat ggcaagtgct taactcaaag gcctacgttc
    25621 cccaaaaccg tgagcggatc ttccttatcg gacattctcg agacgcatgt accgaacaag
    25681 tatttcctat cattggatcg tctccaacat ctgatcaaaa catcagaaac ttattaaata
    25741 tcaacccttc caatcgagga atggggggac aagtctatgg gtcagatgac gtagcgccta
    25801 ccttgaccgg tgatgaagga atcaaaatcg cattgccggt agatgatgga atttctgtag
    25861 cgggtatgtt actgggaaat tttgaacaag gcaatcgggt ttatgagata actggaacag
    25921 cgcctacttt gtcaacgaaa caaggcggaa cgaagatcat gcttcggaaa aacgaaacag
    25981 aatatcaaga ggtgaagcca gggaacagtg tgaacctggc ttttcctaat tccacaagtc
    26041 gtcgaggccg tctgggaaaa caaagtgtgc ataccttatt aacaggagac caacaagcag
    26101 tcgtcacgga tcagtatcaa attcgaaagt taacacctag ggagtgctgg cggcttcagg
    26161 gctttccgga ctgggctttt gatcgagctt ctcaagtcaa ttcagatagt cagttgtata
    26221 agcaggctgg aaattctgta acagttccgg tcatttttga tattgctaga agattaaagg
    26281 aggtaaaaac agatgattta tgatgagttg attggggaat ctactgggtg attggcaaga
    26341 ttcagtcaga tccggaattg gaagaagaac tgtaccggct caattttgat attcgcaaaa
    26401 acggggtgaa agtccctgat gatccgtatg taattgatga ggaaacggat gctcgaatgg
    26461 agctgaatca agtcatcagt gaattggaac gaattgcgga ctttgctaaa gaacctgata
    26521 tccgccaata cttgtttgag ataaaagcag agttggaaat tgagggtatt accgcggagt
    26581 aacggccaaa taaaccactg ataggcactg accgcggggg atttgcgaag cagaaatcct
    26641 ccgtggtgct gtgcctgttt atggacggcg ttagccggcc atgatgtctc accccccgta
    26701 tctaacaggg gggtacaaaa acacaaaaaa taagcaagca acgataagga atggctgtcc
    26761 tatcaaggat aataagcaat ttgtgtagat gttaactgat ttgaaaaagt taacacctta
    26821 aatttggagg tgagaacttg gcacaaagga atttagatta tcggctatta aaagaccggc
    26881 gtaatgaata tggcgtttca caaaataaat tagctacgac ttgtggactt agtcgcccgt
    26941 acttaaatca gatagaaaat ggtggcgtaa ccgcatcgac aaaaaccatg aggaaaattt
    27001 ttgaccaact ggaaagtttc aatcccgagt tacctttaac gttactgttt gactatgtaa
    27061 ggattcgctt tcccacaacg gatgcgagga aaatcattca agagattctc catttgaaat
    27121 ttgattatat gctccacgaa gattacgcct tttactccta tcaagaacaa tatgttatgg
    27181 gagatattgt ggtgatgttg tcacatgaag aagataaagg cgtcctttta gaattaaagg
    27241 gtcgtggttg tcggcagttt gaaacttttt tactcgccca aaagcgtagc tggtatgact
    27301 ttttcgaaga ttgtctaaaa gccggtggcg tgatgaaacg cttagacctg gcaatcaatg
    27361 atcgagttgg cctgttagat attcctgatt taacgaaaaa atgtcagaag gaagaatgta
    27421 tctccttgtt tcgtaccttc aaaagctatc gttccgggga actgttgaaa cctgatgata
    27481 aagatggtat gggcaatact ttgtatattg gcagtcttaa gagtgaggtc tatttctgtt
    27541 tgtacgaaaa agactatgag caatatatca agttagggat tcctttagac cagaccgaaa
    27601 cgaaaaatcg ttttgaaatc cgactgaaaa atgatcgagc ttatcatgca attcaagatt
    27661 tgttaaaagg tcgcagtatt gaaagtacca cattttccat tattaaccgg tacttgcgat
    27721 ttgcggataa ggtggaaggc aaaaggcgaa ctaactggcc cttgaatgaa caatgggggc
    27781 gcttcattgg acggaatcag aaggaaattc aattaacttc tgaaccgaag ccttatacca
    27841 ttgaacgaac gttgaactgg cttggacgac aagtcgcccc tacgtggaag atggccaagg
    27901 agttggatcg gttgaatcag acaacgtaca ttcaggatat ggtaaataat actcgactat
    27961 cagaccggca caaaaagatt ttggagcaac aaagtatggc aattgaaaat ttgattatat
    28021 gaaaagagga atcgtgatgg aaattgtgaa agatatttta ttggtgtttg gtggcagtat
    28081 gcttggtgtt actgtgatgt gcttgatgca tgcaagtgca gcggcggacc gagcaatgga
    28141 aaagaaagga aagtgatgaa atgaatttcg gtcaaaattt gtatcaatgg tttttaacca
    28201 atgctcaatc tttagtctta ctagcgattg tggtcattgg tttgttttta ggatttaaac
    28261 gcgagttttc caaactgatt ggcttcttgg tgattgcttt gattgcggta ggtctggtct
    28321 ttaatgcttc tggtgtgaag gatgtcttac tcaatttgtt taaccgaatt gtcggtgcct
    28381 gagggggaat cacaatggaa cagatacgtg tttatattgc gaaccttgga aaatacaatg
    28441 aaggtgagtt ggtgggggca tggtttaccc cacccgtcga ttttgatgaa gtgaaagaac
    28501 ggattggatt aaatgacgaa tatgaagagt acgcaattca tgattatgaa ctgccttttg
    28561 agattgatga gtatacacca attgaggaaa ttaatcggtt gtgtggttta gcggaagagc
    28621 ttaaaggaac gccaattggt gaggtcgctt cagagattca acatgccttc ttcaattctt
    28681 tcgaggaaat ggtagagcat gtggatgata tcatctatta tcccgattgt aatgacatga
    28741 gtgatgtagc gtattacttg attgtagaag ctggtgattt aggcgaagta ccagaacact
    28801 tgaagacgta tattgatttt gatgcgtgtg gtcgagactt ggagattggc gggaattatt
    28861 tggttacgag tcgaggggtt tttgaactaa tgtaaatcat ttgttagaat tgaccagaaa
    28921 tttctatcgt acttttactt ttctatttga tatactgaaa tagaaaatat ttgttgatag
    28981 gggtgttact aatggttggc aataagtggt acaaatgtga tcttcatatt cattcaactg
    29041 cgagtcagtg ctttcaggat aaaaaggtta ctgctaagga atgggtagaa gaatgtttaa
    29101 acaaaggact cgattgtgtt gcattaactg atcacaatac aggggaaaat attgatgaat
    29161 ataagaaagt ggctatcgaa aatggcttag atttttttcc tggagtggaa ttaacgtgtg
    29221 gagaaaatgg aactcattta ctagtcctat ttgataaaag tgcatgtaca aatgatatct
    29281 ctgatttctt aaccaagatg ggtataaaaa gagaggattg ggccagcgct gcagcttgct
    29341 caatgaaatc agttttagaa gttattgatt ttgctgagca cgagggcttg ctcgttattc
    29401 ctgcacatat agatgagttt aatggaattt cttcagcaaa atttggaaat ctaggaaaga
    29461 tatttgaatc agaaaatata tatgcagtac agacggttca aaaagaattt tttgaaaatc
    29521 gctcacaaat gattccctca tctaagcgtg acactatcta tgatagtgtg aatgccagat
    29581 atgatggtag agttggaaaa gatactttgg aatcttggta taaatcagtt gttgaggcag
    29641 aaaaaaataa tatgacattt cttagctttt ctgataaccc acactcgaaa ggaaattcaa
    29701 aacatggact ttggggaata ggcacaagat attcctatat aaaaatgaag gacgaacctg
    29761 acttgggatc acttcgcgat gctttaatgc taggcgaaac tcgagttcat tctgattttt
    29821 ctaatttctc cattaatgaa aatgaagttt tattggaaaa attaagcttt tctgggacca
    29881 ctcttagcac aaaagaagtt gtagtcgaat tcagcgataa tttaacttca attattggag
    29941 gaagaggaac aggcaaatct tgtattacaa gattcttggt ctatgtcctt ggaaaggaag
    30001 cagaacttga tcaattttct gaaatacaaa gtgattatca gaactttgca caaattgaac
    30061 ataatggctc tggaatattt ttgaaggata caattgtgaa actgaatatt ttctataagg
    30121 ggacaaaata tcaaataata agaacacagg atagacattc tatttatgaa tttacacaag
    30181 agaatggtct ggttgaaaaa gaaacagaac ggttgagaat gatttccgat aaggtgagta
    30241 tatactctca aaaacaaatt tatgaaattt caaaaaatca aactagtata ctagaattac
    30301 tagatggata taactctgat ttgatttcag agtataaaaa tgagattgaa acatgtgtaa
    30361 acgaaataag aaaattaaat tgggatattg tttctgtgaa aaaagaaata caagataagg
    30421 cgaaggtaga gcttgaaatt gaagatttaa aaaaacaagt agaaaagcta tctcataaga
    30481 gttataaaga tgtttatgat gagtattcaa aagagacaga tatttatagg gaactgaaac
    30541 gtgatgtgga ggcacttaag gaaattccca aaaatctaac tgatagtgtt gacaaaattg
    30601 actttggcaa tggtataaaa gttacagacg aaattgatga acaccgcgga gaattgatta
    30661 agaatttagt gaccaatcgg gctaagattc aaacaataat taatgaaatg tcagatgaaa
    30721 ttgatagtta tagagcagag ctgaataaat cagattggaa agttaactat aatgaggttt
    30781 caaagaggta tcaaagaggt atcaaaaact tagtgaagag ttatctcaag atgaattaaa
    30841 aatgttacaa aaccttgatt ctttatatca atctttatca aaaactgaag agcgattgga
    30901 agaaattatt caacaagaaa taaagcttaa agattaccaa gttgaggttg actcagcaat
    30961 gtcaagaatg aataaatcat atcaaaattt ctattctgag agaagtagtt cctcgcgcga
    31021 aattttttct gatattacag gaatagaggc tactattcaa ccaaataggg atttcaataa
    31081 ttatgttaaa gagctcagag cttgtattaa taaggaggac acttttgaca gtgagtttgg
    31141 atctcttatt gataagctga caaaaaattc cattactaat caagatatat ataatgacat
    31201 acttgatata agagttagtg gggaaagtag tattttttca gataaaaaat taatcaattc
    31261 tataagaaaa ttgaatgatg cacaagttct tgaattacaa acccttaagc caaatgattt
    31321 agtagttatc cgacttgaac taaatggaaa aaaagttagt ttatcgaatg ctagtgctgg
    31381 tcaaaagaca tctgcaattc tttctatgat tttggcgtat ggaaattcac cactcatatt
    31441 ggatcagcct gaagatgatt tggatagtca attaattaat aacttaatcg taagaagtat
    31501 cattacaaaa aaggaaaaca gacaaatttt aattgtaact cataatgcaa atatcccagt
    31561 taatggtgat tcagaatggt tgattagcat gggagattca aaagaaatat cggctgatca
    31621 gtgcgggtcg gtagatgaac ccaatatcaa gccaagaata tgtactgtga tggaaggtgg
    31681 cgaggatgct tttacaaata gagcgaaacg ctatggtttt aaacaaattg tagaataaca
    31741 ctagagggtg agacatatta ttatgtccgc cctttttagg aggaaaaatg aaaaaaataa
    31801 aaagctatac cagtatctgg gcagttgaaa aagtcattta cgcaatcaat gattttcagt
    31861 tgccttttcc tgtgactttt aaccaaatgg cttggttcgt tttatcttta ctgtttgtca
    31921 tcgtatttgc ccatgtgcct ccactatcta tgatagagga ggtcttttta aaatacttag
    31981 gcataccggt ggctgtcact tggtttatgt cgcaaaagac gtttgatggc aagaaaccac
    32041 tgggattttt acgatccttt atcagctatc acttgcgatt taaggtgact tttgcaggaa
    32101 agaaagtcaa agaacagaag aaacgatggg atgaacccag tactcttgta aggagtgtga
    32161 actatgtatc cgattaagta tattgaaaat aatctcgtct ttaaccaaga aggcgagtgt
    32221 tttgcctact atgaattagt gccttacaat tactcctttt tgtcacccga acagaaattt
    32281 caagtgcatg ataactttcg ccaactaatt gcgcaaaatc gtgaaggcaa gatccatgcg
    32341 ttacaaattg ccacagaaag tagtattcgc gccacacaag aacgatcaaa aaaagaaatt
    32401 accggtagac tcaaagaagt agcgaaacaa cgaattgata tacagacaga tgcattagta
    32461 tccatgattg gcgatagtca gattgattat cgctttttta ttggctttaa actaattgcg
    32521 acagatgaag aagtcaatct aaagagtctc aagaaatctt tcttttctgg gcttcaagaa
    32581 tttgtctatg gagtgaatca tcatttgatg ggcgattttg tttccttatc caatgaagaa
    32641 attcgctgct attctaagct tgaaaaatta atggaaagca aattggctcg aagatttaaa
    32701 gtcaggcgag tcacaccgag tgacctgacg tatttgattg aacatattta tggcgaaaaa
    32761 gggataccgt ttgaagaata cgaatttcag ctaccaaaga aaaagctaaa atcagaaaca
    32821 ctggtgaaac gttatgactt attacgtcca aatcgttgtt taattgaaga aaaaccaagg
    32881 tgcttacgga tggaacatga gaatcatgag tcgtacgtag cgtatttaac aattaataca
    32941 attgtggggg agatggaatt tccttcctca gaacttttct attaccagca acaacaattt
    33001 acattcccaa tcgacacaag tatgaatgta gagattgtga cgaataaaaa ggctttagct
    33061 accgttcgta ataagaaaaa agaactgaaa gatttagaca atcatgctta ccaatctgac
    33121 aatgaaacga attccaatgt cttagatgct ttggattccg tggatgaatt ggaaaccacc
    33181 ttggatcagt caaaagaatc tatgtacaaa ctgagttatg tggttcgagt gagtgccgaa
    33241 tcagtggatg aactaaagcg acgctgtgat gaagtgctcg atttttatga tgataccaat
    33301 gtaaaactag tccggccctt tggcgatatg atggggctac atgaagaatt cttaccctta
    33361 agtaaacgat atatgaacga ctatattcag tatgttacgt ctgatttttt ggcggggctt
    33421 ggttttgggg ctactcagat gctaggagaa ttagaaggga tttactttgg ctacaatgtg
    33481 gatacaggtc gtaatgttta cctgaaaccg gcattggctt cacaaggagt caagggttca
    33541 gtaaccaatg cgctggccgc cgctttcctg ggttctcttg gtggaggtaa atcctttagt
    33601 aacaatctct tagtctacta tgctgttttg ttcggtgg
//
LOCUS       DACEQH010000029        31750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.029, whole genome shotgun sequence.
ACCESSION   DACEQH010000029 DACEQH010000000
VERSION     DACEQH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.029"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            363..731
                     /locus_tag="IW833_001428"
     CDS             363..731
                     /locus_tag="IW833_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1307556.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="IW833_001429"
     CDS             911..1852
                     /locus_tag="IW833_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ1307557.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="IW833_001430"
     CDS             1921..2652
                     /locus_tag="IW833_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ1307558.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="IW833_001431"
     CDS             2796..4976
                     /locus_tag="IW833_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ1307559.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="IW833_001432"
     CDS             4996..5478
                     /locus_tag="IW833_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ1307560.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="IW833_001433"
     CDS             5494..6255
                     /locus_tag="IW833_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1307561.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="IW833_001434"
     CDS             6386..7720
                     /locus_tag="IW833_001434"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ1307562.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="IW833_001435"
     CDS             8027..8512
                     /locus_tag="IW833_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ1307563.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="IW833_001436"
     CDS             8505..9188
                     /locus_tag="IW833_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ1307564.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="IW833_001437"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="IW833_001437"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ1307565.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="IW833_001438"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="IW833_001438"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ1307566.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /locus_tag="IW833_001439"
     CDS             12804..13433
                     /locus_tag="IW833_001439"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ1307567.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="IW833_001440"
     CDS             13485..13817
                     /locus_tag="IW833_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1307568.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="IW833_001441"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="IW833_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ1307569.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="IW833_001442"
     CDS             14643..16016
                     /locus_tag="IW833_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ1307570.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="IW833_001443"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="IW833_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ1307571.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="IW833_001444"
     CDS             17071..17511
                     /locus_tag="IW833_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1307572.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17655..18356
                     /locus_tag="IW833_001445"
     CDS             17655..18356
                     /locus_tag="IW833_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ1307573.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18353..19474
                     /locus_tag="IW833_001446"
     CDS             18353..19474
                     /locus_tag="IW833_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ1307574.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19489..20721
                     /gene="pepT"
                     /locus_tag="IW833_001447"
     CDS             19489..20721
                     /gene="pepT"
                     /locus_tag="IW833_001447"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ1307575.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20853..21761
                     /locus_tag="IW833_001448"
     CDS             20853..21761
                     /locus_tag="IW833_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ1307576.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21796..22068
                     /locus_tag="IW833_001449"
     CDS             21796..22068
                     /locus_tag="IW833_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ1307577.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22079..23125
                     /locus_tag="IW833_001450"
     CDS             22079..23125
                     /locus_tag="IW833_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ1307578.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23376..25985
                     /gene="clpB"
                     /locus_tag="IW833_001451"
     CDS             23376..25985
                     /gene="clpB"
                     /locus_tag="IW833_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ1307579.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(26136..26807)
                     /locus_tag="IW833_001452"
     CDS             complement(26136..26807)
                     /locus_tag="IW833_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307580.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(26800..27294)
                     /locus_tag="IW833_001453"
     CDS             complement(26800..27294)
                     /locus_tag="IW833_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1307581.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(27314..28534)
                     /locus_tag="IW833_001454"
     CDS             complement(27314..28534)
                     /locus_tag="IW833_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1307582.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28681..30516
                     /gene="lepA"
                     /locus_tag="IW833_001455"
     CDS             28681..30516
                     /gene="lepA"
                     /locus_tag="IW833_001455"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ1307583.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(30610..31737)
                     /locus_tag="IW833_001456"
     CDS             complement(30610..31737)
                     /locus_tag="IW833_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1307584.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        10569 a         5669 c         6794 g         8718 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgagtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtattact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17641 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17701 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17761 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17821 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17881 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17941 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    18001 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    18061 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    18121 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    18181 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    18241 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    18301 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18361 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18421 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18481 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18541 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18601 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18661 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18721 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18781 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18841 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18901 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18961 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    19021 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    19081 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    19141 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    19201 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    19261 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    19321 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19381 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19441 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19501 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19561 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19621 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19681 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19741 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19801 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19861 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19921 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19981 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    20041 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    20101 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    20161 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    20221 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    20281 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    20341 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20401 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20461 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20521 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20581 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20641 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20701 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20761 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20821 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20881 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20941 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    21001 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    21061 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    21121 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    21181 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    21241 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    21301 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21361 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21421 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21481 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21541 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21601 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21661 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21721 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21781 gttggaagaa gagtaatgga tgtattgtta cacaatcttt tgtggtttta ccttttcatt
    21841 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21901 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21961 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    22021 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    22081 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    22141 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    22201 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    22261 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    22321 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22381 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22441 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22501 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22561 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22621 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22681 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22741 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22801 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22861 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22921 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22981 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    23041 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    23101 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    23161 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    23221 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaaccatgtg
    23281 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    23341 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23401 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23461 tcgacattgc ccatgtatgg aaaatctttt tacaacctaa tcattttggt cgaaatttct
    23521 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23581 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23641 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23701 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23761 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23821 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23881 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23941 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    24001 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    24061 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    24121 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    24181 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    24241 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    24301 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24361 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24421 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24481 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24541 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24601 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24661 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24721 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24781 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24841 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24901 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24961 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    25021 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    25081 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    25141 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    25201 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    25261 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    25321 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25381 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25441 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25501 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25561 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25621 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25681 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25741 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25801 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25861 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25921 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25981 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    26041 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    26101 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    26161 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    26221 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    26281 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    26341 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26401 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26461 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26521 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26581 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26641 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26701 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26761 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26821 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26881 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26941 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    27001 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    27061 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    27121 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    27181 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    27241 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    27301 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27361 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27421 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27481 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27541 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27601 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27661 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27721 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27781 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27841 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27901 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27961 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    28021 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    28081 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    28141 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    28201 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    28261 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    28321 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28381 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28441 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28501 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28561 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28621 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28681 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28741 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28801 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28861 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28921 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28981 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    29041 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    29101 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    29161 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    29221 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    29281 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    29341 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29401 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29461 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29521 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29581 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29641 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29701 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29761 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29821 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29881 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29941 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    30001 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    30061 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    30121 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    30181 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    30241 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    30301 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30361 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30421 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30481 gttctgaaaa tggacgagga cgaaccgaag aaatagcaag ggtttgtttg aaaacagtgt
    30541 aaaaaataaa ccatgtttac atggatttgc caccggtttg ccaccggtgg tttttttcgg
    30601 tggcaaaaat caagaattct ttctaagatt ttccatgata tcgaccgttt catttctcat
    30661 cttatctgtt acatgcgagt aagtatccat cgtaattgaa attctgctgt ggcccaatcg
    30721 ttcagagatt tctttcattt ttgcgccatt ttcgagaaga agtgtagcat gagtatgtct
    30781 gagagaatgg aagttaaaag agagggagag tgcattcgat atcctcctag tattccattt
    30841 cactacactt ggcgtaacta gctcaccgtc ttccttcgta catactgcat ttgaatcaat
    30901 gtagagcttt ccatacttca ttcgattttc taattgttgt ttcttatgtt ttttcagaat
    30961 tgctagcaag gtttgtccaa taaaaatcgt tcgattggaa ctgcttgtct ttggcgtacc
    31021 atatacccat gcgccgtcat tctttaccat ttgtttctct acagtaattg ttccatttga
    31081 aaaatcgaca tcatcccacg tcagaccaca aacttcgcca acgcgcattc ccgtataaaa
    31141 tccaatattc aaaggaatat agaaaggatg tccttcagga gtaatttcta gcatatgatc
    31201 aaagtcctca agagaaatga tttttagatc ttttttagtc gttggtcgtg cttcatattt
    31261 tggtattttt acatacagca taggattttg cttgattaat ccccaaggat aaaccgccat
    31321 attcagcgca ttcttaagga cagagtgagt aatagtcatt gttttcttcg agtaaccctt
    31381 tttaaattca gcattgatga aattttgtaa aagagcaggg gagagatccg taagtttttt
    31441 ctttcctaaa taaccgttta tatgattttt gatggtaaat cggtagtttt cataggtatt
    31501 gtattttaga tttagtttaa cgtattcctc catccaaaaa tcaaggtatt gttttactcg
    31561 agtatccgta cctaaaaagt attgtcctgt ttcgtcaata tctgataaaa cttttcgtaa
    31621 agcagcttcg gcctctggtc gggtgtctcc gccaactttc tccacttttt ttcttgagcc
    31681 atcatcattg atatcttcgc cgtttgtata attaagcgag acaaataaaa aagccattta
    31741 tgttacactc
//
LOCUS       DACEQH010000030        30987 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.030, whole genome shotgun sequence.
ACCESSION   DACEQH010000030 DACEQH010000000
VERSION     DACEQH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.030"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            31..270
                     /locus_tag="IW833_001457"
     CDS             31..270
                     /locus_tag="IW833_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307585.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            397..1086
                     /locus_tag="IW833_001458"
     CDS             397..1086
                     /locus_tag="IW833_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAQ1307586.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            1092..2258
                     /locus_tag="IW833_001459"
     CDS             1092..2258
                     /locus_tag="IW833_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ1307587.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            2340..3011
                     /locus_tag="IW833_001460"
     CDS             2340..3011
                     /locus_tag="IW833_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1307588.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            3064..5040
                     /locus_tag="IW833_001461"
     CDS             3064..5040
                     /locus_tag="IW833_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1307589.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            5053..5805
                     /locus_tag="IW833_001462"
     CDS             5053..5805
                     /locus_tag="IW833_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1307590.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            5821..6582
                     /locus_tag="IW833_001463"
     CDS             5821..6582
                     /locus_tag="IW833_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307591.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            6595..6855
                     /locus_tag="IW833_001464"
     CDS             6595..6855
                     /locus_tag="IW833_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307592.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            6852..8942
                     /locus_tag="IW833_001465"
     CDS             6852..8942
                     /locus_tag="IW833_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1307593.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(8978..11035)
                     /locus_tag="IW833_001466"
     CDS             complement(8978..11035)
                     /locus_tag="IW833_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ1307594.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(11180..11371)
                     /locus_tag="IW833_001467"
     CDS             complement(11180..11371)
                     /locus_tag="IW833_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307595.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            11559..12014
                     /locus_tag="IW833_001468"
     CDS             11559..12014
                     /locus_tag="IW833_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307596.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            12011..12253
                     /locus_tag="IW833_001469"
     CDS             12011..12253
                     /locus_tag="IW833_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307597.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            12271..12573
                     /locus_tag="IW833_001470"
     CDS             12271..12573
                     /locus_tag="IW833_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307598.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            12642..14696
                     /locus_tag="IW833_001471"
     CDS             12642..14696
                     /locus_tag="IW833_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307599.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            14696..17308
                     /locus_tag="IW833_001472"
     CDS             14696..17308
                     /locus_tag="IW833_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1307600.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            17305..19683
                     /locus_tag="IW833_001473"
     CDS             17305..19683
                     /locus_tag="IW833_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307601.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            19744..20286
                     /locus_tag="IW833_001474"
     CDS             19744..20286
                     /locus_tag="IW833_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307602.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            20364..20696
                     /locus_tag="IW833_001475"
     CDS             20364..20696
                     /locus_tag="IW833_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307603.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            20697..21290
                     /locus_tag="IW833_001476"
     CDS             20697..21290
                     /locus_tag="IW833_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307604.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            21312..23336
                     /locus_tag="IW833_001477"
     CDS             21312..23336
                     /locus_tag="IW833_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1307605.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVCGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            23336..24535
                     /locus_tag="IW833_001478"
     CDS             23336..24535
                     /locus_tag="IW833_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1307606.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            24551..25195
                     /locus_tag="IW833_001479"
     CDS             24551..25195
                     /locus_tag="IW833_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307607.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            25206..26012
                     /locus_tag="IW833_001480"
     CDS             25206..26012
                     /locus_tag="IW833_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307608.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            26035..26264
                     /locus_tag="IW833_001481"
                     /pseudo
     CDS             26035..26264
                     /locus_tag="IW833_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26423..27358
                     /locus_tag="IW833_001482"
     CDS             26423..27358
                     /locus_tag="IW833_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAQ1307609.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            27402..27671
                     /locus_tag="IW833_001483"
     CDS             27402..27671
                     /locus_tag="IW833_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307610.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            27947..28222
                     /locus_tag="IW833_001484"
     CDS             27947..28222
                     /locus_tag="IW833_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307611.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            28264..28686
                     /locus_tag="IW833_001485"
     CDS             28264..28686
                     /locus_tag="IW833_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307612.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            28706..30298
                     /locus_tag="IW833_001486"
     CDS             28706..30298
                     /locus_tag="IW833_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307613.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            30319..30654
                     /locus_tag="IW833_001487"
     CDS             30319..30654
                     /locus_tag="IW833_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307614.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            30800..>30987
                     /locus_tag="IW833_001488"
     CDS             30800..>30987
                     /locus_tag="IW833_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307615.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT        11144 a         4821 c         6299 g         8723 t
ORIGIN      
        1 ttgcaaaaaa caagaaaggt aggaaaactc atgaattatg aagaaatcga aaacagaaaa
       61 aaagtgtcga aagaaatgga agaaaagttg ctaaaaatga tgaaacaaaa acaccttaaa
      121 agattatctg tcatgcaata tatcaatgat atgaagataa caggaaaaga gaaagcttgt
      181 ttgttaggtt caatgaaaaa ctttgaacag ttgaggcgaa cttatgtaaa aacaggttcc
      241 aattgccaac tattattaga agtttcttga cgattatgat atgaaaatga tcaattatga
      301 tgtggcattg ttattgaata aaaagaatag taaaatttaa catggtttat cttggttaga
      361 tgtaggtaaa ttaattcata tatagaaggt agatagatga ataaaaacaa taaaacttta
      421 tttaatcctt acgtgggcgt aatcattgtt tttcttcttt ttagttattt tgcttatcaa
      481 ggttatcaaa aggacaaaat cgctgaaaaa gtagatggtg taacaaaaac aatccatatt
      541 caggaacagg ctaaaaccta tcaagataat ttaaccagtt taacggatat ttctaagaga
      601 caactgatta ctaatcaaaa acaggcatta gaaaaggatc tcaaaggtga aattgctgga
      661 aaacttgttt tttcttctac aggatttatt ctccctgttt ttcaaggagc gaatagcgac
      721 acgcttagtt taggagtagc ttctacttat tatctagata ctgaaatggg aaatggaaat
      781 tatattcttg ctggttataa cgtagataca ccaagcgttt tattatccga tatgactgat
      841 ttatctgaag gagaagaaat tgatctaatc gatgctcatt acaagtatca gtatgaagtc
      901 tccaaaagag gaaaaatttc tgattacagc caggtagttc aatctaagga aaacaattcc
      961 tttttatcct tgccaaaagc aggtaaaaaa ccactactca cactgttagc ctttcaagca
     1021 aataatatga aaaagtgcga tgtgatccag tgtgaattag tcaatgtaga aaaaatcaat
     1081 aaataaggga agtgagtcac attcaagaac ttgtagaaaa attgatcaat aaaatgagtg
     1141 ccatttttct gttactgttg gtatccattc tatatttaat atggacggtg gggttaaatc
     1201 ctcctacgat attatttatt tatgataatg ctgcacagtt agtgacgttt ttcgtaggct
     1261 gtttgtttct tttgaatctg tacaaggtaa agagaaacga tctattcttc attgcagcag
     1321 gcattctttt ttaccttttc ttttcttttt ttagaagcta ccgaggagat actttctatg
     1381 gtgatctgat cgttccgaca attattgcta ttattttgat tgtaaaatgg acgaaattta
     1441 ctgtaattga tcgtagcttg tatcttgtag tattctatgt ctttttactc gtaacggttt
     1501 atcgagtatt tactgagata ccagtaccag aaggacaaag tatttggaat ccatcgaaca
     1561 aattatcaga tatttggatc aatacaaata cgataggtag ttctttaatg acattagccc
     1621 ttcttatcac aggatttgct agttcatttg gaaaatggta catgcgattg ctatcattac
     1681 cagcgttgat tcttgctggg gcaacgattt gggtttgcca atcgaaagca gcattatatg
     1741 cgcttgttat cttcattatc ttagaaattt tacctaagaa ggtttttcga aatacacact
     1801 tgtccttttg gatgtatgca gcagtttcgg ttctagcttt accgatttct tattttgctg
     1861 caatgtcaga caaagttcac ttatttacag gacgagaaga aatttggcgc aaattctatg
     1921 aaacattagg cgaaaaaaca ggacaaatat ttgtagggat gaaacctttc tttttccata
     1981 gaggaaatca gattctaggt aatcataata cgtatcacgc actcgtcaat cactatggat
     2041 tgattggttt tgttctatcg attattcttt tgcttagttt cttatggttg ctcgtaagaa
     2101 aacgtgagct gacaaatgga caactaacat tcttgtgggc ttttttagcg atcatgtgtc
     2161 aatcgttcat ggaagaaaca ttaacgacaa tcagttgggt gccaattgta ttcttaatgt
     2221 tagcaatggc tacacaccaa tataaaacca ccagataaat gaaatagaat agtaataaga
     2281 gaaaacactt tttcgttgtg aaaaagtgtt ttttgttttg tctgaaagga ggcagatgga
     2341 tgaaaaaatg gttatttggt tttttaggag tcgctttaat cgtcgtttgc agtgtgtttg
     2401 gctatgttag ctaccaaaaa catgaaggag aggtgtttaa gcaaaatata gaaaaaaaga
     2461 tgcccgtaga tcaaatcaat gcacacgcaa agagttataa ggaggatgca acgaacgtca
     2521 ataatgatat gtctttgggg caaatgttgt ccatacaaaa agaagcaatc gaaatgggcg
     2581 tcaataaaca agtatttgca caaattcaga ttcctgcact tggtttagca ttgccaattt
     2641 ttaaaggggc aaatcaatac actttgagtc ttggagcggc tacttatttt tatgaagatg
     2701 ccgaaatggg taaaggaaac tatgtcctag caggtcataa catggaaatg ccaggtgttt
     2761 tattttctga tattcaaaag ttgtcattag gggaggtgat ggacttagtt agtaacgatg
     2821 gcgtctatcg ttacaaggta acgcgtaaat ttatcgtacc tgagtacttt aaattaatcg
     2881 atggcgttcc tgaagagaac tctttcttgt ctttaccaaa gaaaggagag aaacccttat
     2941 taacgttgtt tacttgtgtt tatacgtcac aaggaaaaga aagatacgtg gtacagggag
     3001 agctacaata acaagaattt aatcaacgcg agtcgttgaa atatgaaatg aggagagtaa
     3061 aaaatggcaa taaatcggta tgtaaaaaaa gctggaatga ttgttggaat gttaggaatc
     3121 attgcaggct gctttcaggg gtatcgagct tatgcagaag atgtcacgca aaaaacgcca
     3181 ccagaaaggg tcaatatcac ggttcataaa ttaatgtacg atcaaagtac gcaattgaac
     3241 gttgatattg acggaatcaa aaatgatggt tatacgcacg atcaatatcc tacaggtgta
     3301 acgaaataca acaaggcaga ttatggtgat gttgagttta cgttaggaaa tattacggat
     3361 caagtattac cgactgaaga ttctgatcta accaacgcga aagtggacga aatcgttaaa
     3421 gatgtagaag acaagggatc taacagtgag tacgttaaga atgcaaccaa taaaattact
     3481 tcagctgtag atgaaaatgg tgagatcact tttgctgatc aaccagccta tgtcaattcc
     3541 aaagggaatg tatatgtggt ttatgaaagt aaatccgctg ctggattagt cacacaaaaa
     3601 gcaaaaccaa tggttgtgat tgcgccaatg acagacaaca caggatcagg atttttaaaa
     3661 gatattcatt tgtatccaaa gaacattgtt tctaagttga gttttgaatt aacgaaattt
     3721 ggtgacgatg gcacagctca aagcaaacaa acacctttaa aaggggcaaa atttgagtta
     3781 tacaagggcg aacctggaaa aggaacaaaa ttaggagagc ttgtatcgga tgatcaagga
     3841 aagctgactg caactgattt gacgttagga aaatactact ttgtggaagt tccttcagaa
     3901 gttgtagttg gatcagatca agaaccaaca gcagaccaat atttgttagg cgcagacgct
     3961 cgcaacgatg cgcacaataa gttaaccttt gaaattacga atgacggcgt gacaagtgat
     4021 ttaaaagctt cctacgtgaa ctataaagca ccagtgatcg ataaaacagt gacaaatgga
     4081 acaggagaag aacattcttt ccaaattggt gacgcagtga attatcaagg tacaattcat
     4141 atccctacag atatcgcagg tggaacagat gggattacag taaatggtgt aaaaagtgaa
     4201 acttctcctt acagtgtatt taaatggggc gacacagcag gtaaaggact ttcttatgtt
     4261 gctgaaaaag cagatattaa agtaacaaat aatgatggtt cagtcgtatt aaaagaaaat
     4321 actgactata aaatcgaaaa ttctgatagt ggttttgtga tcgattttat cgtaaataat
     4381 ggacaagtaa gtgatacagt tgcgaattta catggtcaag atctaaaaat gacttataac
     4441 atgtttgtga atgatagtgc tgaaatcgca aatccattaa caaatagcgt ggatttcgtt
     4501 tataacaata atccatttaa tcaggaagaa caccacgaaa aaacgaaagc tgacgttgtg
     4561 acttatggcg caaaattctt aaaagtcgat agcggcctat ttggaacagg tatcaaagca
     4621 acaccgcttg aaggcgcaga atttgcagct aagaatgcag aaggtaagta ctacggtgga
     4681 cttactgata cagataaaga tggtgtcaaa gaagcagttt gggtagacga tgtagcaaat
     4741 gcagcaatcc taaaatcaga taaagagggt cattttgaaa tcactggttt aactgaagga
     4801 gattactcac tggaagaaac aaaagctcct gaaaactatc aaaaattaac aaaagagatc
     4861 tctttcaaag tagacaaaga ttcatacaaa gaagaaaacc gaatcacgat caaaaataat
     4921 caaaaagctt ccgtaccaat gactggtagc aacggtttcc aatcttacgt attgatctct
     4981 tgcttgttgc ttggggcagg cgcaatttct gcagttgttt actttaagaa gaaagcctag
     5041 aaggatcagc taatgattat cagacaccct aagaaaaagc gaatcatggg taaatggatc
     5101 attgctttct ggttactttc agctgttggt gtcttacttt tgatgcctgc tgaagcgagc
     5161 gtggcgaaat accaacaaaa tcagcagatt gccgcaatcg atcgaacagg aacggcagca
     5221 gaaacggatt cttctttgga tgttgctaag attgagttag gcgatcctgt tggtattctt
     5281 acaattccaa gtatttcttt aaaactacca atttatgacg gaacttccga caaaatttta
     5341 gagaatggtg tagggattac ggaaggaact ggcgatatta caggaggaaa cggtaaaaat
     5401 cctttaatcg cagggcacag tggactttat aaagataatc ttttcgatga tttgccttca
     5461 gttaaaaagg gagaaaagtt ttatatcaaa gttgatggtg agcagcacgc ttatcaaatc
     5521 gatcggattg aggaagtgca aaaagacgaa ctacaaagaa actttgtcac ttacttagaa
     5581 ccaaatccca atgaagatcg agtaacgcta atgacatgta cgcctaaggg gatcaacaca
     5641 caccgtttcc tagtgtatgg gaagcgagta acctttacaa aatcagaatt aaaagatgaa
     5701 gaaaataaga aacaaaaatt atcttggaaa tggcttttag gaagtactgt ttttctttca
     5761 gtcatgatca ttggttcgct atttgtttat aaaaagaaaa aataaaggag ggtaaccaac
     5821 atgagaaata gaaaatggct atttgtttgt gcagccgctc ttttttcttt cactgcctac
     5881 acacagttga cgtttgcaga cagacaagaa agcacggata gcacagaaca aacaatccaa
     5941 acgtcagata gcagcacgat tcaaagcagc gatgaaaccg tcgattcgtc aacagttgat
     6001 tcttcagaag aacctaaaat tgctgaagac gatcaaatag tcaaacagac cattcacgtt
     6061 caaaaaatta tttcttccga acaattagac aaccaggggc aaaagcttga aaaacaagaa
     6121 ggagtaaatg gcgcgaagtt ttctgtttat gatgtatcgg acattttaca aaagatggat
     6181 gttaaggatc tcactactga ccaaattgaa tcccaattaa aagatcgagt gaaaaaacta
     6241 tccagtgatc aactgaagct aatttctaat ggagaaacaa agacaattga tcaacaaaca
     6301 ggtgtctttg aattctcggt agaagtacag gcaaatcaga aacaagctta ttatattgtc
     6361 aatgaatcaa gtcctgaaaa catttctaat agcgaggata ttttgctatt aacacctgtt
     6421 tctgataaaa atggtgaatt cttaaaggat gtgtggattt atcctaaatc agaagcttcg
     6481 caacctaaag aagaagtaaa aaaaatcgta tctactggcg taaagaaaaa cttctttgag
     6541 aattgttggg attttgttac tcacttgttt tctagagatt aaggaagaaa taaaatgaaa
     6601 ctgatcaaat tattattatt caatttttct tgtttaaccg cagtcttttt gtactgcggt
     6661 ttattatttt ctaaactatc tattttgcca agctaccaac tatacctaat gaggatttta
     6721 aacataccag ctgcttccat gccgctagat attatggaaa agctcattag gaatccatta
     6781 tttactacga tcaatttgat cgttattgtc ggaatttcac tagtcattta taaaaaagag
     6841 gggatgaagg gatgaatttt aaacactcgt ggacaagacg gttaaaatat gtttttccca
     6901 taatgatgat gttaggtaca ttgtttgggt taaaaacagc caatgtcttt gctgaaaaag
     6961 ttattgtcga tccgtcaaat ccaatccaga acgtaaagaa taattttatt attccaaaag
     7021 atgttcctaa tgcaaaaaca aatattactg taaacggagc gtcaaaagtt tacaaatatc
     7081 atgttgatgc caacaaaggt ggttttacag acgactacgt aggattagtg gagggaaaca
     7141 aaaatatacg ctatccttct ttccaaaaag aatacggtga aacaaacctt gttttagaag
     7201 gcgtaacaac caacaccaaa gtcaatattg actatggaaa aatcggtacg tataacggga
     7261 aaaaagtcaa tatcaagcta gttctatcta atattcactt atactcggat acgttgccgt
     7321 ggaatctact agataacaac tatacaaaaa ctcattttcg agacgatggc tataaaaata
     7381 cgaatggtgc aatgagtaaa tctaaaaaaa gaacagtact ttggatctca gacaatcttt
     7441 tctcaggaat tgtttaccat tcaacacaaa tgaatgtgca gctagtagcc acatatgagg
     7501 acggtagccc cgttcaattt tcaggagata catttatcag ttttaactct ttgaaccctg
     7561 caggaggaaa aagcacggat cttaaaggag aatatgccca ctatgacaaa atgaatacga
     7621 cagattggta tgtcagaaaa gatacggttc tttcagaatt taaatctttc tacaacaatc
     7681 tgaacgtggt aggtggccac ccaggaggaa gctctaagct gacacaagcg gataatgatt
     7741 tcaataattt gcatgataaa ttaggagatc cgaagtttgg tcaaggtact gtgtccttta
     7801 aaatatctga agcaaatccg acgtttgtga ttggttcttc taatgttcaa acctggttta
     7861 ctttatcttc tgcgacaatc tttagtgttg ttcctgatca acctgaaaaa acaggggttg
     7921 ataaaaatgg aaataacgtg aacgataaaa tgttgcaagt aggagacacg attcaatatc
     7981 gaatcaaaca gaaggtgaat cgattaggag tggatttact agcggtttac gatcgatttg
     8041 agttaatcga taatttacca aaagaagtaa attacgtcga tgcacacgtt gaaagtggta
     8101 caaataaaaa attcgatgta tctggtgaag tgacctatga caaaacaaaa caccaggtaa
     8161 aatatgcagc gaaagctgat acattaaaaa atcgaatgaa gtataacggc gaaacttacg
     8221 aattagtgat caatgtaaaa gtcaatgaat tggccaatca aaatagtgta gccaaaaatc
     8281 agggaacgtc gatcattaat aaagtagaaa aggatacaaa tatcattacc gtttatttcc
     8341 ctaaaattcc agtgaaagaa gtacaacaaa atggaaaaga tgtaaacgga cgaaatgacg
     8401 gtaagaaggg tacgccgaca gctcctttaa acgcaggatc tgaagtacaa tatctagtca
     8461 cacaaaaatg gcacacaaaa ggcgtagacg ctgcgtcaga tcactacaaa caatttagta
     8521 ttcaagatcc aattgaagct cgtttgacat ataaagaagg ttctgcgcaa gtaatagata
     8581 agagtaaagg aaaagatatt acttctgaag gcacactaac ctatgatagc aattcaagaa
     8641 cgctgaagtg ggaagcttcc gctgacttct taaataacaa tcttttagat ggacgagaaa
     8701 ttcagctgat atttacagct aaaactccat tacaatcaga aaaaaatatt gataaccaag
     8761 ccgtagtagc agtagataat gtttctaata aaacaaatgt tgtgacaatt ggggttgatc
     8821 ctaacttgcc acaagttatt gttcctaaaa caggttctac acatttagta acgattttag
     8881 cggtcagctt actattactt gtgttagcaa cttttggcta cgcagttctg aagttcaatt
     8941 aaacaaaaaa acaaggctga aaatcagcct tgtttttcta tttgaaaatg ggattggcac
     9001 taataaatac gttgtctgaa gaaagtgatc cgtctgcaga aacttcataa gcatgggata
     9061 aaccaacgat actttgattg ttgtaaagca agtttgcgtt atgagcagcg ctatctttcc
     9121 acataaccat agcatttact gctcctgtat ctgaaaggat ctcgtcaacg taaaggtaga
     9181 aactattgat attttctcct ttagcaccgt agcagcctaa gctttctaag agatcttgga
     9241 aagtaccgcc gccaggtcgg taatgactga agcttacttg taattctttt gcacgaatat
     9301 ctgcagcttg ttgaacgata tcattgaatt gcattggggc aacgccattt tgttgacggt
     9361 agctatttaa ccaagcaagt tctttttgac gcacatagtt gtttaaatca cttagtgaga
     9421 tatttttgta tttgaaagtg atcgttcgat tttggtcaac attattgatt acacgttgtc
     9481 catttttatc aacggtaaca gatgaatcgt catttctaca caagaagtag ttttgatcgg
     9541 tcatattttt ccaagaagta gagaaactat ctccctcgta aaccgtttgt gtttcagtag
     9601 ataaaacagc gtttgtgtca acgtttatat ggttcacagt gacgttgtat tgatgagtat
     9661 aggcaaaatt gattccttga ttacttgtaa cgttgtttaa agtcacagtg ttgctagagc
     9721 tttctctagg atcaagaaca aaattagtga acataggtgc ttcaattgtg atgctttgtc
     9781 cataaacgac gggataactc ttactgtaat tatagagttc ttggatattt ggatcagtat
     9841 ccgagacttt atttggacga gctttcactt caactagaaa gacttcatca tagtaaaaag
     9901 taaatgtggt atttgtgtta atcgtttgtg tttgtgtttc attcccattt acttgatact
     9961 gtggtgcttc aaagttagtc aagtcggttc tagcttttgc agtgtaactt gtaccagctt
    10021 ttactgagac atgttcttct tttagcagcg ttccagtttg tcgattcata tgacggacag
    10081 ttaccgcata ttctgaaggt ttaggtttgt gttgtaaccc tttaaatgca gaatcaagac
    10141 tatttttagc attatttact tgggtttgag aagctttaga atcgtctagt acttttttag
    10201 cattggataa agcagtctga aaattattcc aagatccttc tgtaaaatct cctttagcag
    10261 ttgattttac ttggttgtac aaggcttgta attccgtttt gttcacagta ggagctggtt
    10321 ttttctttaa tcccttgtaa gcagaatcta atgtgttttt ggcactatcc acttgttttt
    10381 gagtcgcttt tgagtcattt aacacttttt tagcattgtt taaagcggtt tggaatgcgt
    10441 tccaggtatt gtcggtatag tcaccttttt tagttccctt aacgctcgta taaagagctt
    10501 ctaaagctga tttattcact ttgggtttag aggtgtcagc accataccaa ccaatacctt
    10561 cgtatttcca gccatagtct ttggttaacc attgtacttc tttccaactt gcagtgtagt
    10621 gatgcgaacc agaggtaaca cctggaagga aaaggcggta gatcggttta ttattttttt
    10681 cggcagaacg ccaggcaggc ccttcataag tccaaccgta atttttggtc aaccatttca
    10741 cttcatccca actagctgtg taatggtgat cacgaaggcc tttattgtat aaacgataga
    10801 caaggtggcc accgctagtc ggtgcttccc aagcggggcc ttcataatta ccccacttaa
    10861 ttttcactag gtgatcacgt tcttttgtgc tagaggtgta aaaatgttca tggttatttg
    10921 gattataaag gcgatacatg acgttagttg aagcagcgtc agcagatcca gcaagagcaa
    10981 ctgtggcagc aaatgttcca atagctataa cagtttttgg gtttaataat ttcatgtttt
    11041 caactcccta ttaaatgatt aaatattttg tttacattca taatatacca cggtataccg
    11101 aggtagtcaa cggtataccg tggaaatttt gaaataaaaa agccgcccta tgaagagtgt
    11161 aaggatggct gtttatatgc tatgaagaaa tggattcggc gtatttggtt agtttggcag
    11221 cattatcaaa tcgcattttt tcaatcgcac tttttttctt tattaaatcc gcaattgttg
    11281 attctgcgac tgaagaatgt ttgctaattt catatgctgt cttgccagtt tcaaatagta
    11341 accattcgat tttctcttta tcagcgtaca ttgcaatgct ccctcttgat tttttcttta
    11401 ttatagctct tttttgtaat aaatcacaat atcaagagga aaagtttcta tagaaaggtg
    11461 ggaaattatg agtatccgtt ccccgtgtat caaaatgagg tttgttgcta ctttcgttat
    11521 ttaatatact aaaataaaac aacgaaagaa ggaatagaat gaaaaagtta ataatggtat
    11581 ttacttcgtt gattgccatg attgttattt taggtggatg tggtagtgaa aaaaataagc
    11641 aagatagtca agataatggc gtgttaaaac caggaacagt gtggaaagaa gaagtaggcg
    11701 gacttgttca taatttaaaa attatagatg atacaacatg ggaatattcc gaaagtgttt
    11761 ggcatcctga acaagtacaa ataacagttg agaagctaaa agactataaa ggattagaaa
    11821 gatataaggt aatcgattca ggtggagtag atttattcat aggtaaaaca gccttaatag
    11881 ttccctataa caaagacggt ttagaaacgt ttaaatttat tccaacaaac gaaaaagaac
    11941 atcgagataa aagtgaaata ttaaaagaat acagtactaa atctggatac aagttacaaa
    12001 agactagcga gtgattgcta gtctttttat ttatcctgaa ggaggaatcg cagtggcaaa
    12061 agctaaaacg ccatttgaaa aagccgcgtc taaaatctta gaacgtcgcg gtgtatctga
    12121 aacacagtgg aaacaagaag tccttgaaaa agcacaattt gacctattac ggggaaaaga
    12181 caaggactta gaaggatata tctatcaacg tgaacgtgaa aagcttgttt tcgatgaaat
    12241 tattaaacac taaaaggagc gaacaatttt atgtttgaat caattacaat gaatttttta
    12301 acacaaccga aagtagtagc tgcggttgat atttttagag cattacaagg tgcagcaact
    12361 agtttaaaaa acaatctgat gatttttgcg gctgtagctg ccgttgcctc cattgttctt
    12421 ggggggattt tcttcttctt tggccgaaga ggagctgaaa acgggaagat gcacgtaggt
    12481 tatgtattga tgggggtagg agttatatta gctgccgcct ctattgtggt ttcgttattt
    12541 ggaatgtttg gacaaacccc acagggacta taaaaaataa atagaaggag taaaaagtac
    12601 gtggctactt tttactcctt ttttatgaaa ggagaatact catgtacttt acgatttcag
    12661 gcaataccaa aaaagataaa gagttaataa aaaaagcaac gaagaaagtg ggaaagcgta
    12721 ctgccttttt accgaaaaca attaaatatt ctgtcattga aacggaagaa cagctacatg
    12781 tgttaggaca agtgttagaa caagagaatc aatcttctgt taaattgact ctcacgttac
    12841 atgataaaga aggaaaaata gaaaaggctg gcacagaata cgtttttggc ccactggatt
    12901 atcaaatttc agaaggtttt tatcctacat tgatggattt gatccgcctt gatgtgttta
    12961 aggaaacgga tgaaaccatg agtgtggaag agtcttatcg aaacgaaaaa ctgctacaag
    13021 ttttagaaga agagttagag agcgaccaaa gaaaagtgag tcaagtgagt gaagtcgaag
    13081 taaatgatga agtaccatgg gaacgaccaa tctttccaaa gaaggagtca aaagaaaaag
    13141 agacagtcta tgttgacgaa caagctgata ttccgactca gaaatcgata tttgaggaag
    13201 agaacgatgt aatgggtcaa ttggaggtag atccctttga agaaacaatg gaggatgaag
    13261 agacggccga agtagaagtt gatctagttg aagctaatga agagccaaca tggactgaag
    13321 gggattctca ggaatatatt gaaccactta tcagtgaaga agtagagaaa aacgaaacag
    13381 agatacaaaa ggcaactgta tttgattata ttttggatca atatccgcca gagcatgaat
    13441 ggatgaaaga aaaaaataaa ttatttttaa atgagcttta ttcgaaatat caattacccc
    13501 aaacctatca agcattttta gagagtaaag aagaattaat ctcacagggt aaaggcacac
    13561 ttttagaaca gttagagtct gttcaatcaa caaattggca tgagcttgca caagaagatt
    13621 tattagccac ttttgaagaa cgtcaacaag aaacagatca agaaataaat gagtatgcga
    13681 cggaacagtc atctaattgg gagagaacaa aagaggaact aaataaagaa gaacaacgag
    13741 tgattgaaga agaagtacaa aaaattaaag agcgatacga aaagaaacgc gacaatgcgt
    13801 attccttgtg tatagagaaa attaataact ttactaaaac taatcaagaa gctttatcga
    13861 aagaaaaaga aaacttgtta aaagaacgta taaaagaacg aaaagaagag ttctaccgag
    13921 gtttacgtac agataagcta aagattagca aagaattaaa tgaacaactg aataaacttt
    13981 atcaaacaac gactgaagtt cttatggaaa gacacgggca gatccagcaa gaattacaag
    14041 cacaaattcc aaaatggaaa aaagagtatg aaattcaaca acaagaattg aaagaacaag
    14101 aagaaaaaca acgacaagaa gaaaaagagc gcgaacaatt agaactaaaa aaacgtgagc
    14161 agcatcttgc agaacaaaaa gtggcgatag aaaaacaacg gttagagcaa gaaaaggaaa
    14221 aagaagcagc tgctcgtaag gaacgtcaag aacaaatgga tacattgcga caagcacagt
    14281 tgaattatct agcacaacct caaccagtag ctgctgttca aacgccagtg aacaaccaag
    14341 caaatttaac tccttcacca cagcgttcct ttaacggatg gatggtggga tgtattgcaa
    14401 gtttaagctt gcttctaggt ggaggaacag tatttgcttt taatcatatc aatcaatctt
    14461 cacaggcaga ttctgtagcc acaacggcgt caatcaagga agaagcccag gaacaagcaa
    14521 gaaaagagta tgaacaacaa ttggaagaat tattgaatcg taccaatcag tcaacagcag
    14581 agtcaagtat ggaaaatagc aaagagagtg atcctagtaa ggctaaagaa ggatcaagcg
    14641 attcaactgt acactcaaac acttccgaaa gttcagaatc aaatgaggtg aaataatgcg
    14701 tattagagaa acaacgagac gaaaagacaa acgattcaat ttagaaaaga ataggtatct
    14761 ttctcatttc tatcgctata ctccttcagg agaagagcta ccagaaaaac atgacaaacg
    14821 tatttctcaa aagatttttt atagtggctt agcttatctt cctttaaact atgttgttac
    14881 aagaatacca gaagcatttc atcgatttga gattattcca ggaagtttac aaggagtgaa
    14941 tatttttcag ttacaagaaa tgccgcagtt attccatttt gattcaactg gtttttggaa
    15001 tcctttgacc ccaacaacaa gtttaggcac acgtttagtt gtttctacga tttgtgttgt
    15061 tccagctgta cttatgggat tgaaaatgga catgtggttt cgtccgctag cagatgggca
    15121 aaaaggagat aaccgattaa aaacgttaaa ggaaatccaa caaatttatc cgcaaatacc
    15181 agaatcaaag ggaagttttc ctggttatgg cggaatacca attactcatt ataaaaaata
    15241 ctggtatatt cagacggata cagtaaatac ttgtattgtt ggtacttccc gctcagggaa
    15301 aggccagata gaagttttag caaccattga caatttgtcg cgtgcagaaa gacaaagttc
    15361 aatggttgtt aatgatccaa aaacagaatt atatgttgct agtaaagatg aactagaagc
    15421 tagagggtat gatgtgtatg cctttaatat caccgatcca ctgcaatcaa tgagtgataa
    15481 tccattggca ctcattgtga aatactggaa gcgaggagac atagacaccg caacacagtt
    15541 aaccaatact tttacgaata cgatttatta tgatgcaaat gcttcagaca ataaatactt
    15601 taacgaaaat gcgcagaaag ctgtcaatgc gcttattttt gcgttattgg aacaagcgga
    15661 tcaaacaggg gattactcca aattaacacc aaataatctc gttgaactac tttcagaaat
    15721 tggcggtttt aattattatc cagatcctga gaatgaattc aagcagctca atgccttaga
    15781 tgagttcatg aatcagttac cccctggaaa tatagcaaaa aaacaatatg gggcaacaaa
    15841 aattggatct gataaagcga aagggaatat cttgtctacg gcaattacag gattgaatcc
    15901 atttacccta acgaaaattg cgaagatgac ttctcaatcg accattgatt acaagtccat
    15961 tggttttccg aagtatttgg atctcaaatt acatgaaagc ctattaaatc aacgcattgt
    16021 cgtggaattt tatcataagg gaaagaaatt tcacgaggaa cgagtcaaag ttggctatcg
    16081 tggtttttgt gaaatcaact ttgattgtca tttagagcag ggagatcatg tttgcttaaa
    16141 atacaatcat caaggaaaac aatatgttgc ttggtatgag ttttctcaaa aagtattgaa
    16201 agacgaagag ggaaaaatcg tttatcgaaa aaaacgagga gaaactcatt tgccagaact
    16261 agaaaaagaa gccgttctaa cattagatga gaagaaaagt aacctattag tagagtctat
    16321 tcggatgcac tataccgata aaccaatggc tatttttatg ttaacgcctg attatgatcc
    16381 atctaaccat atcattgtta gcatctttct tagtcagttg tataaagaat tatctacgca
    16441 atgtgttaag actaaaggag ataaatgtca ccgtaggatt cattttattt tagatgaatt
    16501 tggaaatatg ccaccgatgg acaacatgga aggaatcatg acggttacgg caggtcgtaa
    16561 tatgttatgg gatctgttta ttcaatctta tcagcaactt tatgcgaagt atggagaaca
    16621 aaacgcagcg attatcaaag aaaattgtca gacacatatt tatattatgt cgacagatga
    16681 taataccatc gaagaatttt ctaaaaaagt ggggaataag acggttgaac aagagaacag
    16741 taccatcaat tcattaggta tgaatactca tatcaatcgc aatgttgatt cagatcgtat
    16801 cttaactcct gaaagaattt ctactttttt agaaggtgaa acgattgtac tcgctccttt
    16861 aaaacgacaa gatttgaaag gtcataaaat acggccgtat cctattttta atcatgggga
    16921 aacgaatctt ccttatgctt atcaatatat tgaagatttc aatccatcgt gggatatcaa
    16981 tgatatagac atacattgta cacatgcgga cttggatctt aaggctttac aaattgatta
    17041 tgattatttc ctaccagatg aatatgctag aaatctctat catggactgc atgatcaatt
    17101 agaagaaaaa aacgaagaaa atatgtttta taaaatcgtt caatcttttg agaatgagac
    17161 ggctaaaaaa ttgttaattc aattttacga agaacaagca acggatcgtc ttcaagaata
    17221 tgttgaaaag ttgatcaaaa atgaagaaat ttctgaaatg attaggaaac ggctgttatt
    17281 ggaagctgaa aaggcggtga aagaatgatc attggaggaa ttttctctgg catagccgac
    17341 tggtggtcag gtttattcgg agaaggggat gcagatccca aaaccgtaac gaaagtctta
    17401 caagattatg gtaattatct gcagtatcaa gatgtattca gttggatttg taatactttt
    17461 ttatggggat tgatcaaagg gttatataat ctcaatactt tattagaaaa aacaatttat
    17521 caaagctttg atttaaaaga cttattgaac gccgctggtg ttaatgaact atttcaaagt
    17581 ttgattagta aggtattggc tcttttttgt gtcgtcacat taatttattt aggcttgaaa
    17641 ttttttatga gtaaacaccc accgaaagtc aaaaatatct tggttaattt atttatggcc
    17701 atgatcttga tttttggtgg tagttcttta atcgatgaag ggttaaatat cagtaaaaac
    17761 ttttatggag acgtcactgc agcaaataaa gcagataaaa cgggttcacc tgcttttcaa
    17821 attattcaaa ataatgtgat tgatgtcagt acattactag cgactgaccc gaataaagta
    17881 gaatccttac caacagataa aagaaatgct ttaacagcga aaaatttcaa aacagcgaat
    17941 atcaatggga ttattacacc tgatcaagct aagaaaatgt ctgaatcaag tgatattagt
    18001 acagtacaaa aagatcggtt aaaggcatta cagtatcgac tagagttaga cgataatggg
    18061 aaagaaatcc ctgttcaaat tagtgatgaa ggtttagcaa aatatgtgta tacgtcaggc
    18121 tatcgtcgat acattagtag tccagggatt attttggctg gggaatcttc tttgtcggta
    18181 gcctatttat ttattttgtt tacaatcgtt acttgcatta ttgagctagt atttaaaaaa
    18241 ttctatttag taattgccgc gtcaacggac tttgagacag gacaacggat gaaaagtgcg
    18301 atagaagata tttctcaatc cttcttgtta ttggcgttta cgattttaga attacgaatc
    18361 tatacattaa tgctttcagg tatcggtgat ttgcatgcgg taggaaagat caatggattc
    18421 ttgtatgttg ttgctttaat tgtgctaact attgcgctat tcaaaggctc tcaatcggtt
    18481 acgaagattt tcggtgttga tacgagtctg aaaaacggca gcaattctct tcttagcatg
    18541 tttgccttag gaaatattgc gaaaaatgtg ggtagcggag ctaaaaacct tgcaagtgct
    18601 ggtaagagtg gtttaagcca tttaaatgat attcgtaaga atggatttgg ccatcgaaaa
    18661 gatattaccc atgataacgg ggaagaacaa tcagacgata aagctacaac tgtacctaat
    18721 agtaagaaga cagtttccga aatgttcggg aaagcgaaaa atggtgtttc taaagccgca
    18781 gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa cagttgaaga tcttacagaa
    18841 aaagccaaag acaccttaga agagaaatta gacggcacga gagctaaaga atttgctgaa
    18901 gctgtgaaga atccatccga agcactagaa aaacaaaaag aaaaagcttc agcagtcaaa
    18961 gaaggactaa aagatgcgta tggtgaagga caagtcaatg cagcagtgaa ggcaaataca
    19021 acaagtgaaa aagaaaatca agaaggtgca gcgattcctt cagccaaaga tcaccaagat
    19081 gagttacatt ctaacgaaaa ttcgatcgga gaaaaagatt cagaagaagg acaacatggt
    19141 cagttaccaa aaaccgatcc agaacaatat cgttataaat ctaacattaa ttctgaaaac
    19201 gaaaaacgca tttcagattt accaaaacga aatcaagaag tacaaacgga tgaggattcc
    19261 atcgatctgc caaatgataa tggcatcgaa gttggaactg aaaaatcaac gaaaattggt
    19321 ttgcctaaga cacaaaattt accaatgaaa gatagtcgtt tgaatcaatt caaagagaat
    19381 gatcaaagct ttgttcatca atcacctgca ggtactgttc aaatgaaatc aagtcgtttg
    19441 cctgaaaatg gaaatctgac aactgtttct tacaatttag ctgaaagtgg tgaaacagag
    19501 aatgcgtccg ttcaaaatat tgtgacaaaa gaagctgcac ctaagccaat caataaagtt
    19561 cataatggat acagaccagc aaacgtagca tttactgcac cgacaaaaat aaatgtgaca
    19621 agagaaacta aaaacaggaa caatgaattg aaagaacaac tattgaagga tatcaaacca
    19681 taaaaggcct atttaagggc ctttttcttt ataaaacaag tttaaaatgg aaaggagaaa
    19741 attgtggagg atattttaat agctacgttt ataactgcac tgggagcatt tttaacgata
    19801 tttgtggtga ctattaaagg atttaaagat acggatagtt tacaaaaaga tcatcagtct
    19861 attttagatg gtcaaaaaac acataccgaa agcattattg tacacctttc agaaggattc
    19921 acttatttga aagatgctat gcccacattc acagtaaaat taacggaaat atttcattct
    19981 gttgagcaaa tatcgtcaga attgaaagag caaagacaac aaaaagatcg tcaacttggc
    20041 tcacttaatt ttaggcaaga aacgatacaa gaatcattgg agaatgtaaa tcgattaaac
    20101 gatgaaatgc aacgagttca tttggaaaat gaagaattac gcgcacaaaa ttatgattta
    20161 attcaaaaga aaagaaatct ggaacaaaaa attgttgaat taaaagaaga aaatcgtcaa
    20221 ttgaattatc aattacgacg aggaaaaaat cgttctcgtg atttaggaaa tgaattagag
    20281 ttgtaatatt tagaaaaacg ctgtgagaaa tcacagtgtt ttttatttct aaaaatacaa
    20341 aatataaggg gtatggttat attatggata aatacattca tatgaaaaat cctccaagat
    20401 ttagtgggga atggaagttt ttcgcctttt tctttttaga tctattaacg cttggtggct
    20461 gctttttttg ggcgaaaata gtcaataatc ttgtccactt accactcttt tttgcactct
    20521 tgaattatgg attatggctt gcgatagcgt tagtgattgt ttggcgaccc tataataacc
    20581 ctggtcaacg ccaaattttt gcgattatcg aatctatttt ctttacggat aagaaccact
    20641 atcatgcgtt agatcctaac cgaaattatc atggtacgta taaggaggat gaataaatgc
    20701 gagtcaaagg acaaaattca gttgataaga aatccatttt gcagtttgga aaaccagaag
    20761 agcgctctat cttggactta tttccatata aatcctgtac agaagaggga gtcttaatta
    20821 ctagagaaga tcattttcag cgttattatc gtgtcgtttc cactgacgtg gagggattaa
    20881 atgaacaaga gaaactagaa cgaatgaatc agttgaccac cgtcatgcgt acgtatgtgc
    20941 caacaattaa gttagttagt ttgaccacag agacggatct aacggaacaa atcgtgcaaa
    21001 agcggcagtt attagataaa aatcgtatgg aacagtcact aggaagaaat ctacagcgat
    21061 taagaaaata tgaaaagaaa ttagtagagg agatccagga attaaaacgg gcagaacaag
    21121 aacgtcctga cttaagcttc tttttcgtta ttgaaggaaa gacaatcaag gatttacaaa
    21181 ctagaaatcg tcagttaatg cgatctagtg gcattttagg gttaaaacca ctctacaaaa
    21241 aagaattgat taagattctt tatcgaatga acaacatgaa tgacgaataa aaaagatgga
    21301 ggaaatgatt catgctagat gtgataaaaa cagctttttc aacgcaaagt aatcaaaagg
    21361 tgattgatcc attaaaacaa cttggtaatc gtgaaataga gattttaaaa tcaaaaggtt
    21421 atgatttaga tttcttaaaa caagtgatgc cacaaggtgg gttgaatttt gatgaagaat
    21481 atgtgaccta tggaaatggt tattcacgat gtgtgacagt ttatcattac acaaaaaatc
    21541 ctactctttt ttggttggcc aatctcatga acaatcctca tacagtggcc acactagatg
    21601 ttagcacaga tgaaaagcaa aaagtgattg atcaaatcaa ccgcgcatta gatgaattaa
    21661 atgatcgatc agataatgaa cgaaaagcga ctgaccgaaa taaaagtgcg gaagaatacc
    21721 tggaactagc ggaatatgcc cgaaaattga cttctaatgg ggaaatttct aaacaagtca
    21781 aagtgagagt acatgtttgc ggaaatacga tccaagaagt ggatgaacgg gcgcaggaac
    21841 tgatcgaaaa tctaaaaggg atggattatc gtgcggtgac ttatctgcat aaaaccaaac
    21901 gagattggca atcgatgttt accatgttcg atgaacaagc tgaatggttt ggaaatatca
    21961 aagggcaatc acttccatcg ttaaacattg gcggaggatt cccgttccac caccaaacgc
    22021 taaaagatcc aggaggcaca caatatgggg aaaccatgac aggcggccca tttgtctgga
    22081 atgccacgta taaaaatagt gtacgtttat ctaccaatac cttgcttcta ggaatgatgg
    22141 gagctggtaa atcgaccgtt cttaaaatga tcgcggaagc ccatttagct gcaggagact
    22201 ttatttgggg ctttgaaaag ggaaaagact ttattccttt cttaaaggaa tataacggta
    22261 ttatggtgcg tttagacgga tctgacggga tgatcaatcc tttagagatc tttgccacac
    22321 ggacgtatga tgaagcatcc agtttatatg acgatggttc agtcaaagat ttgaagatca
    22381 atgaagcagc gtcctatcaa acgcacttag acaaagtagt ctatcaggta cagttagtga
    22441 gtcctcagct aaaaggaacg atgaaagcag aattcaagac ctacttgaat cgtttttatg
    22501 aagagtatgg aacagtacca cgcggtttta cttcctcaaa ttcgcgttca aatactgata
    22561 cgcaagtcac agggaaagat ccagaagctt atccgacgtt caaagaattt ttagcctttt
    22621 taggacaact ggaactacca ggggcttccc aagagaaaaa gaatcggaaa gaagaaatgg
    22681 agtctatcgt ggaatcctta tgtgagactt atggcatgat ttttgatggt catagtacga
    22741 ttcgacacct tgaccaacaa cagttggtct tttttgacat tgactctatt acaggcctag
    22801 atcgaggggt acaacaatgt cagatgtaca tggccatgac gttgatttgg aatcaggcat
    22861 tgattcaagg acgaagacaa cgacgcttaa ttaaagaaaa acaaatggaa gaacaagact
    22921 tcaaacgctt cttggcgatg attgatgagt gccataatgc gattaatcca gatttcattg
    22981 aaacggtaaa ttatgtcaca aacttccaac gagaaatgcg taaagtagaa gcaatgactg
    23041 ttttagctac acaatcccca caagaaatgg tgccagaaaa tatgtcttct gataacttct
    23101 cgaaactgaa gaaagtcttt gaattttctt ctacgaagat ctttatgcgt atggatgaat
    23161 ctattttacg tcatatagaa ggattagttg gtaaatcaat gacgaactca gaattagaat
    23221 ccattcctca acaaaatcaa ggagacgctg tgatcaattt tggctcaaaa gaaaccattc
    23281 gtgttcattt tagtccgaac aaacgtcaat taaaattgtt tgatggagga aaataatgga
    23341 aaaccaaaat gaaagtttaa tcaaacagta tgttaagcgt cgtgcaaagc ggcgcttatt
    23401 tttgtgggtg tttggtactt ctgcaggttt gattactata ttgatcactg tgtttgtgac
    23461 cctttttcta atacttgcgg caggttctat tgataattct gattcaagta gtggtggcga
    23521 agcttttaca ggagaatatt cggaaggctt acccatttat aaagaaatca aaggaagagg
    23581 ccctttttct gatgagattg cgcaatatgc tgtaggtgca gcagtgaagt ataaattatt
    23641 accaagtgtg atcctttcgc aatacggtta tgaatcagcg tttggtacgt cggcgtccgc
    23701 aagaaatgat ctcaattatt ttgggattac gtggtttgat gggtgtctgt ttcctaaagg
    23761 aactgcgcgt ggaattggtg gcattgaagg tggctggtac atgaagtttc ccaactcaaa
    23821 agctgccttc tcctattacg gctttatggt agccacacaa tcaaatttta atgcttgtgt
    23881 cggaaataaa agccctggcg caagcttatt gatcttagga cgaggagggt atgccgccgc
    23941 aggaattact gaagacagtg cctattataa aaattgtatg tctattatta atacaaataa
    24001 attgatggag tacgatgagt ttgcgattaa acactggaga gaaggcggcg gaactggtgg
    24061 tacgattaca ggaagctggg gcaatccatt cccaggaagt agcttagata aaaattcgtt
    24121 cagtggagga caactttttg ggaaaaatcc aggaggagaa ttccgaccta acgggttcca
    24181 tgatggacta gactttggtt cagtcgatca tccaggaagt gagattcatg cggttcatgg
    24241 cggaaaagtc gtgtacgttg gcaatcctgg tatttcaggt ttgggtgcgt gtgtgatcgt
    24301 gattaattac gatggcttga atatggtgta tcaagaattt gcaaatagta caggaaattc
    24361 tcgagtgaaa gtaggcgatc aagtgaaagt tggtcaagta attgggatac gagatacagc
    24421 acacttgcat ttaggattta ctcgaatgga ttggcgtcaa gcacaaggac atgcattcat
    24481 tgatgatggt acatggatcg atccgttacc atttttgaat agcagcaaaa aatagatcag
    24541 gagaaaaaat atggaaaata aaaccttaaa aatcgtctta acagctgcaa ttagcgttag
    24601 tttaatcggc aatattgcgt tgttttggca gcttaaagaa gaacaagaga caaataccta
    24661 taaaattgaa gaagtggcta aaaacgcgtc tgataagctc aaaaaaaatt cttctgaagc
    24721 acaggcgaag attgacaagt taaatcaaac catcaatgag aaaaaccaag aaatcgatca
    24781 gctgaagcaa caagatggca caaaagcaaa agaggaagta actgaagcac aaagaaagac
    24841 ggcagaagag ttcggtcaat tggccattga taaatcatta agcagaaatg aattaacgga
    24901 aaaactaaaa gatgtcgcaa cccaagaagt gatcgacaaa ttaagtcctg tagacgatga
    24961 tcaccaacat gatgattacg gaagttactc ttttacatta ggtgacgtgc aaacttacgc
    25021 tcaaacaagc agtgtaaaag aggaacaaaa ttttgtcgta tttgttgatt atacaatcgg
    25081 caatcctcaa ttcaaagatg tcaaaccaca aaaaataaaa ggtggattaa ctgtcactga
    25141 aaaacaagta gatggtcaat ggaaagtcac agactttagt tatttcactc gttaggagga
    25201 agaaaatgag taagaaaaaa ggagcaagac aacgttttaa aaaacgaaaa agttatctta
    25261 gatggctggg gatctttatg attctcggct ttttgattct tgctagtccg atttacacac
    25321 ataaaaaggt agacatacca gatgaagtaa gcacgaacgc atttttaaac acgaaggaac
    25381 tgacgatggt ttcaaaagaa aaaggagcga accagcaatt agtttttgaa tttgcgatcg
    25441 acgatgaaga tcagagctta ttaaaagaac tagctaatct taattatcgt gtagaagtaa
    25501 agacagctaa aggggattat caagcaataa aagccaaagt gatcaaggtg tctgatgatt
    25561 attttgtagt gaagttagca catgtaccag aagaatatat tgctatgcga ttaactatta
    25621 ttcctgaaaa aattgatcca aaggtggata tgcaagaacc gcaagatctg attttttata
    25681 ttcacgaaga taaggtcaaa gatcacgtga aagacgaaga ttatgaacaa catgcgatca
    25741 attacaaagt gaaaggatac aagaaagaac aaaaagcagc acaaaaacaa attgaatcct
    25801 tccaagcaac cattgatttg aatgaagaat tagtgaagaa actaaaagat caattgcctt
    25861 atcaagtcgc agaagatcag gaaaatactg aaaataagat taatagttac cagcaagaaa
    25921 ttgaaacatc gaaaacgcaa atgaaagatc aagaagagag cattcaaaaa cttcaagaaa
    25981 aaattgatcg tatgacaaaa gaaaatattt agaagaatag gtggagtaaa agaaatgaag
    26041 ggagcgaact tggtcggcaa agatactttc gctgagcaga aagtattgat taaagcctat
    26101 ctcaaagttg taaaagctaa agaaatatgt caagaagtga attatctaac acaacaatat
    26161 ctgagttgaa gaatggtaga aagagtttag ataccttaaa attcagatca tttgaagctc
    26221 tatatatagc tgctaaaaaa tattttacag ctgataataa ataaaaaatt aaggtgggtt
    26281 ctatgtgaaa aaaacgtatc aaaagttttt atggattagc tatatcgtcc tatttatcgt
    26341 tgggtttctc gtcagtgtgt ccgtgatcgt cggaacgctt tttcttttgt tctctgacga
    26401 agatctggta cttaaggggt taatgagtac gttatcgtgt gtcatgttac tttatttatg
    26461 tatacagatc aacgaaatta tgccccctca ttggcaagaa aagaagaaag aatttaagga
    26521 agaaatcgaa ataaaatcca atgactggat tgaatacaca tggcttcctc tgaaaagaaa
    26581 tagcaaacga aaggaatttt gggcattgca attatggcca ataggaaaac tttttattta
    26641 tatggcttca cttatggtta ctttaatcgg ttatttggtg gtggtctatg gggtggccta
    26701 ttacttgtta agtgagccaa aacagatcgc ggcgtcaaat attttcacgg cagtaatgac
    26761 attaatgtgt gtcatgtgct tttctatttt tagttactta ctcgtccaat ttatcaggca
    26821 aaatttaaca atgagtaaac ttttcaaaag tttattggac ttacttatct taggcagtat
    26881 gttaacgact ctaacagatc ggttcgaaca atctgaaaaa acggtagagg ggataaagaa
    26941 cttcttaacg acgattgatc aatttataaa tggatatatg tatttgtttg aagtaggctt
    27001 tattctcact ttgtttggtt gtgtatttat aaaactcatt aaaagatcaa tcgaaaaaga
    27061 aatggttatt gatctaattc cgacgctgag cggctatgag tattacgttg attttggaaa
    27121 gtatgaggag ttgatccata cggatatcgt tcacacgagt atccatgaag taaccgttaa
    27181 gggcatcaga tctactttaa aagatggaca ggagacgaaa acaatcgacc gaagaaaaga
    27241 ggtctacaat ttttataaag ttgaagcaag tctacgatat ggcattggga aattgaggtt
    27301 agatgggagt aatattcaac aaaacaaacc atatgtttta ggaaaagtgg tgatttagaa
    27361 cttcctatga aagatgaata aataaggaga atcttttgaa aatgattctt ctttacagaa
    27421 ttgtgcttat agttggcacg gagttaaaaa tcttaaagaa ggagcagtta ttcaaagtga
    27481 aagaacatca taatcagtta ctatgtatta aaacgggtac gatgaacatt caataccgaa
    27541 cagaatattt agaattaacg aatcaaactg attttttgtc gattcgagag caacataagc
    27601 aagaaaaatt aatggctact tatagccaat tgattgatga gtgggaacaa ttcaaggaat
    27661 ctaaaacata aaattccatt taaacacctt attttttaaa gagcataagg aaacgtgtac
    27721 ccactttcaa aataaagtgg gtagataggc tgtgcctatc acgtttctga aaaaaataaa
    27781 tgaccccaaa attttcaatt tatgtgtagg tttatgggta gcaaaaatat gaatgtgggc
    27841 agcaaaaagt tcatttgtgg gtagtaaaat tcaaatgtgt gtagcaatgt gtgtagctac
    27901 acacattgct acacacgcta taacatatag tgaaaaggag atatttatgg aagtaaaaat
    27961 aagagatatt gatgaaaaaa cgattcaaag aattgaccga agatgtattg aattaagtga
    28021 acgcaccggg actaaatgga gccgaaatcg atacctaaaa gttttgattg aaaatgattt
    28081 tgaccatgaa cttatggatc atagaaaaga tcaatttgat cggttgttag aaaaatttgt
    28141 ggatattcaa acttataata cgaaaatttt agaagaatat attgcaacaa ataatcaatt
    28201 aatcgccttg ttaattgaat aataaaaata gtattgtgaa tttataaaag gaggaaatca
    28261 attttggcaa aagcaacaag aaaacatgta tatctttacg atatgcatat cgaagtactt
    28321 gatgaaataa tttctactca tgaagatata tctaattata gtgaagcagt cagacatttg
    28381 tgtatgatgt atgatccaaa aatcgaagaa aaaataaatc caattcaaat taaattgaat
    28441 gcaatgggaa aagaaatttc tatattaacc gaaatggttt cagagtttgc ggatcttcat
    28501 ttgaaagata caggaatact aattggtaca aattcaactg tctatcaaga agcaaaagag
    28561 cgtgtggaag ggaaaataaa agcgaatcaa acgaaaaagt attctcctaa gatcaagaaa
    28621 ccaataaatg aatctgttca tgccagagta gacccgtttg atccaacaaa gtttttaaaa
    28681 agataaaaag tagaagggga acatgatgaa taaatcgcct gcagtcattc tcacttgtca
    28741 atttaccttg cccaataaaa aatctttttc aacatacatt gattatatga caagagaaaa
    28801 agcgctggaa gaaaaggaac gacgcacgcc ggacgaagaa caggaactgc aacgaatcaa
    28861 gaatgcttta gaggattttt atattcctga aggagaaact ttttcggaga gtaagacaga
    28921 taaagaattc agtgaaacgt cgaatgaagc tcaatcgttg atggaacatg gaatcaattt
    28981 tgacaagtta gaggaacaag attttaccaa atatctttct tatatgaccc gtcactatgc
    29041 gttagaacaa aagaaagaac tgacgcaaag ggaacaaaaa gaataccaac ggttgaacca
    29101 agcaatgaaa aaatatcaaa cagagccaca taaaaaagct tcaggaaatc ttcctggagt
    29161 tttttctatg gcttcagacg aagtgaaagg aagtgatctg aaggagatca aacaaatctt
    29221 ccaaagaggg caacaaaaag gctcaatcat ttatcaagat gttgtctctc acgacaattc
    29281 gtatcttgaa aaaatgggct tgtacgatcc caaaacagat accttagatg aagaaggatt
    29341 aaaagcagct ggaagaaaaa tgatggagac attattcgag aaagaacaat tgaatgaaac
    29401 aggttactgg atggcaacga ttcaccgtaa cactaagcat atccatattc attttgcagt
    29461 tgttgaaaaa gaaaatacgc gtaaacctta cacggaggtt gaaaatggtg tgagttatat
    29521 cgtaccgaaa ggtaagagaa gacaagcaac attagacgca atgaagacca attatgtaca
    29581 tgagttagaa cgtttttctt atgaaaagga tcgaatgatt ggtatggaga aacgaaattt
    29641 attgacgcgg aaaagtgatt tgcgtaacac cttaacaaaa gtggtaaaag agccagcgcg
    29701 ttacgatcga cacgcaatga acttattaaa tgaagtatat aaaaccctac ctgacaaacg
    29761 ttcggaatgg cattatgggg atgaaaaacg aaccaaactt tcaccataca caagagagaa
    29821 actcgatgag ttaaccaaat atgtgttaac aaaagaacct gaatatcaag aatatataca
    29881 gatttcaaaa tcattgaaag aagaagcaaa aagtctttat ggggaaagca agagagaatc
    29941 aaaggatgca gaaaaaaatg ctctctttga tttgaagaag cgcacaggaa atgccattct
    30001 aacagaactg aagaaacagg atagtcaaat aaaaccttta atagaaagta tggatcagat
    30061 gcaatctatt tacaatgaac gatcctttcg aacgcttaaa cagttaccaa cgaatgaata
    30121 caaatatagt tacgaagaat tcatgaagcg caggaaacaa ttccaacaaa gagttgtcac
    30181 gcaacggagt ttgcgtcaat taaatcgaac gattaatgcc catgaagaaa aataccacgc
    30241 aagtaaagcc tacgaagagc aacaacgaag aattcaaatg gaacaagaaa gacagtaaag
    30301 acagtaggag gaatagcaat gaaacaatat ttagatcaat ggaaagttat agaaggatca
    30361 ttaagagaag aacgaatcga acaacttccc gattgtttag aaaaagagca tttgtttcag
    30421 ataagagaaa tgttgagaaa cgaacagttt gatccaaatc aatttttagt cgttgagtac
    30481 tcagctactg gtgtgtactg ttgtaatcat gtgaagggtg aaaaatattt tatcattcaa
    30541 gaatatgagg gcaagttagc gccttattat actacttggg aaatgaatga agaggggatc
    30601 aataacttcc cgtgtaaaag cattgaagag tctatttcac tcacagaatg ttagttgtta
    30661 agtgaataaa agaagtaaaa gagtcctgaa cgtaattgtc caggactctt tttttcaatg
    30721 aattgttttc cgccaattca atgagtggtg cgatttgcga ctacattata tcaaaagaaa
    30781 gagaagggag gaagaagaaa tggcttattc aacgattgga gttgttgttg aaaaaagcag
    30841 agacaatctc gtttttgtga cagaaattca aacgggcaga gcttttgtcg tcacagacaa
    30901 agcggcaaaa gcgtatcaaa gtggcgatat tttaacgtta aatctgacta cgaagacgtt
    30961 cgtcgatgcg gcagaagatt atccatt
//
LOCUS       DACEQH010000031        30582 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.031, whole genome shotgun sequence.
ACCESSION   DACEQH010000031 DACEQH010000000
VERSION     DACEQH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.031"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..221
                     /locus_tag="IW833_001489"
     CDS             <1..221
                     /locus_tag="IW833_001489"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ1307616.1"
                     /translation="ALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLIVI
                     IIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            203..508
                     /gene="kduI"
                     /locus_tag="IW833_001490"
                     /pseudo
     CDS             203..508
                     /gene="kduI"
                     /locus_tag="IW833_001490"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            528..1331
                     /locus_tag="IW833_001491"
     CDS             528..1331
                     /locus_tag="IW833_001491"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ1307617.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(1580..2644)
                     /locus_tag="IW833_001492"
     CDS             complement(1580..2644)
                     /locus_tag="IW833_001492"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ1307618.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(2641..3726)
                     /locus_tag="IW833_001493"
     CDS             complement(2641..3726)
                     /locus_tag="IW833_001493"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ1307619.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(3739..4716)
                     /gene="mvaD"
                     /locus_tag="IW833_001494"
     CDS             complement(3739..4716)
                     /gene="mvaD"
                     /locus_tag="IW833_001494"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ1307620.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(4709..5653)
                     /gene="mvk"
                     /locus_tag="IW833_001495"
     CDS             complement(4709..5653)
                     /gene="mvk"
                     /locus_tag="IW833_001495"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ1307621.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5975..6628
                     /locus_tag="IW833_001496"
     CDS             5975..6628
                     /locus_tag="IW833_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1307622.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            6713..7618
                     /locus_tag="IW833_001497"
     CDS             6713..7618
                     /locus_tag="IW833_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1307623.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(7620..8252)
                     /locus_tag="IW833_001498"
     CDS             complement(7620..8252)
                     /locus_tag="IW833_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1307624.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            8572..9096
                     /gene="infC"
                     /locus_tag="IW833_001499"
     CDS             8572..9096
                     /gene="infC"
                     /locus_tag="IW833_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ1307625.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            9168..9368
                     /gene="rpmI"
                     /locus_tag="IW833_001500"
     CDS             9168..9368
                     /gene="rpmI"
                     /locus_tag="IW833_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ1307626.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            9421..9780
                     /gene="rplT"
                     /locus_tag="IW833_001501"
     CDS             9421..9780
                     /gene="rplT"
                     /locus_tag="IW833_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ1307627.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            10132..11370
                     /locus_tag="IW833_001502"
     CDS             10132..11370
                     /locus_tag="IW833_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ1307628.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAES
                     YTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLL
                     PVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGF
                     LMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSA
                     ILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIP
                     TGWIIGAILCLITFLLYGNA"
     gene            11418..13547
                     /locus_tag="IW833_001503"
     CDS             11418..13547
                     /locus_tag="IW833_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ1307629.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            13522..14211
                     /locus_tag="IW833_001504"
     CDS             13522..14211
                     /locus_tag="IW833_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307630.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEIYEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(14619..15305)
                     /locus_tag="IW833_001505"
     CDS             complement(14619..15305)
                     /locus_tag="IW833_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1307631.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(15441..15635)
                     /locus_tag="IW833_001506"
     CDS             complement(15441..15635)
                     /locus_tag="IW833_001506"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307632.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(15685..16125)
                     /locus_tag="IW833_001507"
     CDS             complement(15685..16125)
                     /locus_tag="IW833_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ1307633.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(16129..16974)
                     /locus_tag="IW833_001508"
     CDS             complement(16129..16974)
                     /locus_tag="IW833_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ1307634.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            17123..18541
                     /gene="pepV"
                     /locus_tag="IW833_001509"
     CDS             17123..18541
                     /gene="pepV"
                     /locus_tag="IW833_001509"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ1307635.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(18617..19819)
                     /locus_tag="IW833_001510"
     CDS             complement(18617..19819)
                     /locus_tag="IW833_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307636.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(19806..21593)
                     /locus_tag="IW833_001511"
     CDS             complement(19806..21593)
                     /locus_tag="IW833_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307637.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(21606..22559)
                     /locus_tag="IW833_001512"
     CDS             complement(21606..22559)
                     /locus_tag="IW833_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1307638.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(22744..23094)
                     /locus_tag="IW833_001513"
     CDS             complement(22744..23094)
                     /locus_tag="IW833_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1307639.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            23294..24373
                     /gene="pepA"
                     /locus_tag="IW833_001514"
     CDS             23294..24373
                     /gene="pepA"
                     /locus_tag="IW833_001514"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ1307640.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            24516..24836
                     /locus_tag="IW833_001515"
     CDS             24516..24836
                     /locus_tag="IW833_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1307641.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            24858..25322
                     /locus_tag="IW833_001516"
     CDS             24858..25322
                     /locus_tag="IW833_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1307642.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            25518..26123
                     /locus_tag="IW833_001517"
     CDS             25518..26123
                     /locus_tag="IW833_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ1307643.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(26162..27451)
                     /locus_tag="IW833_001518"
     CDS             complement(26162..27451)
                     /locus_tag="IW833_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1307644.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            27879..28358
                     /gene="rlmH"
                     /locus_tag="IW833_001519"
     CDS             27879..28358
                     /gene="rlmH"
                     /locus_tag="IW833_001519"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ1307645.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            28711..29025
                     /locus_tag="IW833_001520"
     CDS             28711..29025
                     /locus_tag="IW833_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1307646.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            29041..29427
                     /locus_tag="IW833_001521"
     CDS             29041..29427
                     /locus_tag="IW833_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1307647.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            29456..>30582
                     /locus_tag="IW833_001522"
     CDS             29456..>30582
                     /locus_tag="IW833_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1307648.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRD"
BASE COUNT         9686 a         5721 c         6239 g         8936 t
ORIGIN      
        1 gggcgctagg aatcatgcta gtagcattca tcgttgctaa aaagaataaa taccctgttg
       61 tcgaaaaagg acagatggct catgctggaa aaataataaa agaggcgatt cctagccttc
      121 tattgattgt catcattatc ggtggtatct tgacggggat atttacagca gtagaagctt
      181 cagcgatagc tgtcatttat aaatgggtta tacgatttta gaaccaggaa gtgcttggaa
      241 tacgatgcct tgtcatacac atgaacgtcg tatggaagct tatgtctatt ttgattatgc
      301 caatgaagat acacgtgtgt tccatatgat gggcaaacca gatgaaacaa aacatttagt
      361 cgtagacaat gaacaagcca ttatttcgcc aagctggtcg atccattcag gtgtaggtac
      421 aagcaattac tcattcatct gggcaatgtg cggagaaaat atcacctata cagacatgga
      481 tatggtgcaa atggatcaat taaaataagc ataagggaga gaaaatgatg gaatttagta
      541 tggatatgtt tcgtttagat ggaaaagtag cattagtcac cggtgctgtc tatggtattg
      601 gatttgagat tgcacgctct ttagctacag caggagcaaa aatcgtattt aataacttga
      661 atcaagaatc agttgatgaa ggtatagcgc attataaaga agcaggcatc gatgcaaaag
      721 gttatgtctg cgatgtgacc gatgaagaag cggttcaagc gatggttcaa cagatcaaag
      781 aagatgtagg ttcagttgat attctagtca acaatgcagg gatcatcaaa cggacaccga
      841 tgatcgaaat ggatgcggca gacttccgcc aagtaatcga cgtggattta aatgccccat
      901 tcatcgtttc aaaagcagtt attcgggata tgatcgaaaa aggcggcggt aaaatcatca
      961 atatctgctc catgatgagt gaattaggac gcgaaacggt cagtgcttat gctgcggcaa
     1021 aaggcggatt aaaaatgctg acgaaaaaca tcgcttctga atacggtcaa tacaatatcc
     1081 aatgtaatgg aattggacca ggttatattg ctacaccgca aactgctcca ttacgcgaaa
     1141 cacaagaaaa cggtgaacgt catccattcg atcaatttat cgttggacgt acgccagcag
     1201 cacgctgggg cgagccaatc gatttggctg gaccatctgt cttcttggct tctagcgcat
     1261 ctgatttcgt taacggccat gtcttgtatg tggacggagg catacttgcc tatatcggaa
     1321 aacaacctta aaagagcttt agtacaccgt ttagtggaga ggttgtgaaa tcagcggctt
     1381 gaacgaatac cgaaggacag ctgattaaac atcatttatt cgaaaaaaga ggctgggaca
     1441 gaagcgttta actccaagaa ataagaagga attcacgaaa attgcttttc aaatttttgt
     1501 gaatttcagc ttatttccga aggagttgct tttgcttccg ccgtttatac gtgtttagag
     1561 cgtgggataa aagtgttctt tacttttgtc ccacgctctt ttctgtgcgt cttccatact
     1621 tcactaagtc gatccctctt gcttcgcacc aatccttcac tggtccggaa aagatcagtg
     1681 attgttggtg tagggccgcg gtattcgttg cgccgaccat agtataaagc agacgaagct
     1741 cttcttgcca ttgcttcatc agtataatag tttcttctac gccgtgattc atcagatgcg
     1801 tcagtacggt tcctgaagta cccactgcat ttgcgccgag acaaagggct ttaaatatgt
     1861 cataggcatt gcggatacct ccggaggcta ggatttccat agaagtgtct gcttcatttg
     1921 cttcaagcaa tgaagagact gtcgattgcc cccagtcagc taaataactc agttcccgct
     1981 tggagcgacg ggcgttttcg atctgggtga agcttgttcc gcttcgaccg ctgatatcaa
     2041 ctgtatgaac tcctaaagca gctagatcat tgagcgtttc tcttgtcatg ccaaaaccga
     2101 cttcttttac gatgaggggg acgtcaatgg ctgtttgagt ctcttgaata agtactttcc
     2161 aattggtaaa atcacggtct ccctcaggca taaccaactc ttgaggtgca ttcaaatgaa
     2221 tctgtaaggc atctgcgtga aacaatcgga tagcttcttg ggctctttcg acagaagtcc
     2281 ctgcaccgat gttggcaatg attttcccgt gaggatattc ttgtcgcata atcgtataag
     2341 tatcggctaa agaagggtct ttcaaggcag cgctgactga accagtggca atcatcaaat
     2401 ctgcttctct tgcgacgatt gcaagatctt ggttgatttt ttttgttttt tcgcttccgc
     2461 cagtcatcgc attgatataa aatgggctgc ttagtgtaaa cccaactgcc tgtgtactta
     2521 gatcgacatc agaaactgct atttgcggca aagggttatg gatgatccgt acgaagtcaa
     2581 attcgttttt ttgtttatca tgaaaggctt tagctagtga aacatgttcg tcttttcgat
     2641 tcatcggttt tcctttcttt gatcgctata gacatgtaac ggcagtggag tgatttctgc
     2701 tttttcccat gcactcatta aaggaaggat gccagatttc tggtcaacaa ttacgattcc
     2761 gcaatcgcca ccacctgcac cagaagattt tgcggctcct tcatactgtt cagctaaatt
     2821 acacaatttg ttcaaagcag gcgtttcgat gacgacccca gtgattgcag ataaatcatg
     2881 cagtaattgt cggttttttc gaatcatctg ttggatcaac gttacattat tttctttaaa
     2941 ccctttgatc atttcgttga cacattctgt actgtttttt aagaaaagct gataagccac
     3001 cattttgtct tctctcgaac ggtgaacttg atcgaccaaa tcagaagtag aggcagggct
     3061 acccgtccaa ccaatcaata aacgtaaatc ttcaggagca atcaatggct caatggatag
     3121 acctggccaa tccagggcaa gtaactcact gatagaatgc tgagtttctt gttcttggag
     3181 ccaaggatgg tcgaaggttg agaaagcgat ccagccacca taacagctag cagcgatgtc
     3241 gccgcaggaa ccattatctt gaactgctaa attggctagt gctgcaatct tgaaaatctc
     3301 caattgagat aaattcaacg cgtaaaaaac attcaaggct ttgaccgttg caacggtcac
     3361 ggcaccgcta gaacccaatc catatttccg gccatttgaa ctgtctaact cacttgttac
     3421 tttcagatca taaaaggata aaagaatgtt tttttcttgt gcatactttt cagtcaaacg
     3481 aatagcagca aggatataat gaaaaggatt ttcccgaata tctaaaacta attctccgtt
     3541 gcgtcttgtc caacgtacag gcatcccact atattgtgca gattggatac ttccgacttt
     3601 tcgtgcggat tctacagtga ctgttacgaa ttgatcgact gcagcgataa ctgctggatg
     3661 tcctgtttca acaactgcat attctccggc aatatagagt ttgcctggtg cagatacttc
     3721 aatcataaaa atctcccttt attcaataat cgcaattcct ggaccagcat gggcagtaat
     3781 tacttgttct ttgctaaata atttggtaaa tgtttcttgt acctcagata aatggctgtt
     3841 ttccactaaa actttgacat tagggccagc atccatcgta aagtagcatg gaatgccttt
     3901 ttttctcaat tgccgcaccg catccatcgc ttttaagctg tcaggagacc agtaagtgaa
     3961 tggaggctgg gcagctaaag tcgttccatg catttttagt ccgtttcttt ccatcgtttc
     4021 tccgagaagt tggaaatctt ttgttttgat tgcttgtttc aattgatata gatccccttc
     4081 aacggaatct aaccagcctt gataaaaact ggatgtttcg actgttcgac gcatcccatt
     4141 tctgctggac acttcttttt tctggtcgtt gatcaaaacg aaaaccattg ctaaatcgtc
     4201 ttcgaaagaa tcggaaggga ctggcttagc gtaagaactt aagtcgtcat ggcctttttc
     4261 ccattcaacg aaaccaccga aaatacttcg gcaagctgaa ccagacccgc gtcgagcaaa
     4321 tcttgaaaga gagagatcgt ctaatccaag cttaagagca gtattgcaag ctcctgctaa
     4381 agcagctagc ccgctggcag aggaggctaa tccagctgcg gtaggaacga aattctgact
     4441 gattactgaa gcttttagtg agaggccggc ttctttacga aaaaggtcaa gaaactgact
     4501 gacttttttt gttgcttttt cgtcttgtaa ggtgccatct agataaaatt catctaccat
     4561 atagctatca gaaaagatga cttctgtctc tgtataaaat gcatccaatg ttaacgaaag
     4621 gctgttgttc attggaagga tgagttcttc atttttctta ccccaatatt tgattagagc
     4681 aatgttggta tatgcgcgtg ctttgccttt aaacataggt atgtactcct aatccttgta
     4741 tccaagtttc agcagcaccc gcatcttcaa gtgcttgact gatttcttgg gcttttgttt
     4801 ttgtttgtgc taatgcaatc atacagccac cgcgtccacc gccagttaat ttggcaccca
     4861 atgccccggt atcttttgct gtttggatca atagattaag aaaatcattg ctgattgtca
     4921 gcttttccag aagtgactgt gattcatcca ttgtctgtgc caacgtttct ggtgaattct
     4981 caatgattgc ttgttttgct tgcttcgtca agtatcctaa tttttggatc atttgtccag
     5041 tttcatgagg ctgggtttca aagaggtgag cgacatcttt gacggcttct cttgtttgtc
     5101 ctttgattcc tgtatcagcg acaatcaaaa aagcatcgat gttcaaagaa aagtagtcaa
     5161 aaggatgccc ttttgtaaaa taaatcggct gcaagctgct agtagcggct gcatcgattc
     5221 cactaggatt accgtgggcg attttttccg aaagctggac attttctaat agtatttcac
     5281 gagacaaagg aaatgctaag taatcataaa aagcacgagt gactgctgtg gcgactgcag
     5341 cgcttgatcc cattccacgt tcagcaggaa tcgtgctttc aatggtcagt tgcaagtttt
     5401 tttggatgtc atgctgtttt tttaagtgaa tgaataattt tttgatattt tttaatgcat
     5461 gaggagcttc ggctaaattt cctgaatagt aggaagaaac cagttgatcg tccattgcat
     5521 ccagctcttc aaggaatgcg gtgacttttg ttgcatagaa aggaaaggca atcgctggtt
     5581 ctccataaac aaccgcgtgc tcgcccatca atatgatctt tccgcttgat tctccttggc
     5641 catagtttgc cataatctaa ctccttctca cgtaaaagtg atttttaagc aaaaaataaa
     5701 gcgcccaaaa cgagttgcgc tgcttcacga atttttttgt ccactaaggg tctcaaaaaa
     5761 cattcctcct ttattttact aaaaccatct cagaagtaaa actaagaata cgttttctaa
     5821 agaaaaaaca cttttttctt aagaattttt tgactttttt tcttctttga ctagaaaaaa
     5881 tacaattgac actactagat attgggggta tatttaaaaa aggttggagt taaacacaat
     5941 atttagtgat gaagtgagct ggaggtaaat gagcgtgatc gttttagcag gtacaattgg
     6001 tgccggaaaa tcaagtttaa cagaaatgat ggctgaacac tttgatagtc aggcatttta
     6061 tgaatcgatt gatgataatg aagtcttgcc cttattttat gcaaaccctg aacagtatgc
     6121 ttttttgctg caaatttatt ttttgaataa acgttttgca agcatcaaac aagcaatgaa
     6181 agatgacaat aatgtattgg accgttcgat ttatgaagat tcactgcttt tccatctaaa
     6241 tgcagatcta ggaagagcaa cagaaacaga agttcgtgtg tatgatgaac tgctggaaaa
     6301 catgatggaa gaacttccct atgctgccca taaaaaacat ccggatttat tagttcatat
     6361 tcgtgtatct tttgatacga tgttagagcg tatcgaaaaa cgtggtcgtt cgtatgaaca
     6421 attgtccttt gacccaagtc tatacgatta ttacaaagaa ttgaacagac gttatgatca
     6481 atggtatgaa gaatacaaag aaagtccaaa aatccaaatc gatggagacc gctataattt
     6541 tgtggaagat cctcaagcaa aagaagaagt cttgaaaatg atcgaagaaa aattagcaga
     6601 gattcgccaa accaaagtat ccatttgatt tacagttgct caaataaagg gtaaaccttt
     6661 atttgagcga cttttttctt tatactggaa acaaatgtca ggaggataga gtatgcggga
     6721 aaaacactat tttaatcaag accgtaattt ttggatcaat gtacagcaaa acgatgtgct
     6781 agctgttaaa aacttaaaag aagaatatgg cattagtgac gagatgctga cttattctct
     6841 agataaaaat gaacgggcgc gcgtagagta cgattctttt gatgaagcgt tactgcttgt
     6901 atcaaatgtt ccgcaccagc aaaaagtcga taatcattat gaaactagtc ctatcgcttt
     6961 tattttaaaa gaagacggtt tgtttacatt taccacgccg aataccgagt acgtgattcg
     7021 actaattcga tcgcttttgg aacggatgcc agacatgagt gtatacagtt tattgtttcg
     7081 tacattgttt ttgatttctg attccttttt tcctttaatc gaggaagtga atagtgaacg
     7141 tcagcggctt aatctgaaat taagagaaaa aacaacgaac aagaatctgc tgcaactttc
     7201 agatttagag atcggtttgg tttacttagt tacaggaaca aaacaaaatg tagtactttt
     7261 agaacaaatc aaggcactgg ctatttatcg caagttgtca gaaaaggaaa aagaagagtt
     7321 ggacgatgca ctgatcgaag cacgacaagc agttgaaatg acgaacttag cttcgcagat
     7381 tttagaccag ctctctggca cttataataa tttgctgaat aacaacttga atgacacgat
     7441 gaaatttttg acggtctggt cgctgatctt gaccgttcca actattgtga caggattctt
     7501 cggaatgaat ctccagttac cctttaccca ttccgttttc ggatgggaaa tcgccttgat
     7561 catcagtttg gtcttatcta tttggatgct tattgcactg tggcggcgga ttcgttgatc
     7621 tagtctttga tctgcaaacc aagggcttgg gcgatcacaa gtgcttgctc ttgattgacg
     7681 attagtcccg ccaaagaatc ttgagataaa gcgattgttg tgaagaaatt agtcgtaaag
     7741 tctaatccct tcagagaagt tcgaaaccag ttcgaaccgg ttaactgatt gtcagacaaa
     7801 aaaaggtttt tccaagtgac ctcataaaat tcgctgtctt ctaattgaca atgatcgaag
     7861 gagacagctt tcaagtttgt actactaaag gaagccatcg aagcaataca atcagtgaaa
     7921 gtacagtctc gaagataact ttcggcaaaa ttcgtacctg ttaatttaca ttgatgaaac
     7981 acgacttggt ggaaactgcc accaatccac tccagattgg aaaaatcaca tttttcaaaa
     8041 atgacattac tgcattcaaa atgacgtaaa cctgtttttt gcatcgtaag tttttggata
     8101 tgactctctc ttaataccat atttttggca tcgagatagc tttggttacc tccgttgatc
     8161 agaagaccag agaagatctc ctcatcttct aaataaaact ctccctttgt aagaagagga
     8221 agagaaggtg gaataggttc gatttttttc atgtgggttc acctcgtttt tctttagtat
     8281 agcaaagaag cgggaatcac tgcaagaaag cctgctaatc taatcatttt cagaaaagga
     8341 agacaaactg tatgaaaaac attgacaaaa agattagttt ttgctatact ataatggttg
     8401 agaaataaaa gcagaagcac ccgcttctcg ccttagttga taatgtcact gggctagata
     8461 gttttcactg gttctattag gtgaagtacg cgcgggttct gtaagagaac tcgttttttt
     8521 attgccgttt attcggtaag cttttctttc tctcataata tttggaggtg aatgaccata
     8581 gcaaaggata tgatggttaa cgacggcatt cgtgcacgag agttacgatt gatcggttca
     8641 gatggtgagc aattaggagt taagacaaaa gcagaagcat tgaatattgc agaacaagca
     8701 aacttggatc ttgtgttagt tgcccctggt gcgaaaccac cagttgcgcg aatcatggac
     8761 tacggtaaat accgtttcga acaacaaaag aaagaccgcg aagcgcgtaa aaaacaaaaa
     8821 gtgatcaatg taaaagaagt tcgcttaagt cctacgatcg acttgaatga cttcaataca
     8881 aaacttcgta atgcacgtaa gttcttggaa aagggcgata aagtgaaagc ttctatccgt
     8941 ttcaaaggcc gtgccattac ccataaagaa attggtcaaa aagtcttgaa tcgcttagct
     9001 gaagaaactg cggatattgc aacagtggaa caaaaagcga aaatggacgg acgcagcatg
     9061 ttcttgacgc tggcaccgaa aaacgacagt aagtaatttg aatgactaac tggctgttgg
     9121 acagccgata gtcggataac gaaagatttt gaggaggaaa tatagtcatg ccaaaacaaa
     9181 aaacacaccg cggatcagca aaacgtttca aacgtactgg taaaggcgga ttgaaacgct
     9241 tccgcgcgtt tacaagtcac cgtttccacg gcaaaacaaa aaaacaacgc cgtcaattgc
     9301 gtaaagcaag catggtttct agtggcgact tcaaacgtat tcgccaacaa ctagcaaaaa
     9361 tgaaataaga agtacaaata acagaatctg aagatttatt tttattagga ggaattatac
     9421 atggcacgtg ttaaaggtgg aacagtaact cgtaaacgtc gtaaaagagt gctaaaatta
     9481 gcaaaaggtt actacggttc aaaacacact ttatttaaaa cagcaaaaga acaagtaatg
     9541 aattcataca actacgcata tcgcgatcgt cgtcaaaaga aacgcgactt ccgtaaattg
     9601 tggattgctc gtatcaacgc agcagctcgt atgaatggct tgagctactc aaaattaatg
     9661 cacggtttga aagttgctga aatcgacatc aaccgtaaaa tgttagctga cttggctgtt
     9721 aacgatgcag cagcatttac tgcattggct gaccaagcaa aagaagcttt ggctaaataa
     9781 gctaaacaat tataaagagg aaagaagata acgaaaagtt atgttctttc ctcttttttt
     9841 atttacaatc ctccatcaaa tgaaacagga caataacgta taagtctaaa ataacataat
     9901 taaggttttt taattgaaaa ctgaacattt atggtcatcc tttaatgaaa gcgctaacta
     9961 cataagttag aatgagtttg actgatatat tagtttgctt atcagaaaaa caaaatggac
    10021 ggaggaataa catgagtgat attattattg gtattttgtt agttatctca ttctttttca
    10081 tggtctggta ctgtgtaaaa ggctacaact tgatggtcgg cttttgtcat catggctact
    10141 gtatggatgg gattagcgct agtcggaaac acgttttcac caaactcggc gatggaaggg
    10201 caaggagtaa ttgatgtgtt gacgcatatc tatacgacgg ggccagctga gtacgccaaa
    10261 tcgatcttgg taaatgtctt ctttggtgcc tttttcgggc gtgttctagt agatagcgga
    10321 atcgcagcta ccctgattcg aaaggtagtt gaacttggag gggacaagcc aagaatcacg
    10381 atgagcttac tatgtattgt aaccgctgtt atattcatgt cgatgacagg aatcggacca
    10441 gtgatttcta ttgcagttat tgtgttgccg atcttgatgt cattaggtat ttcagtaccg
    10501 gttgcactgt tctcatttat gggctcgatt atggctggaa tctttgctaa tatcgtcaat
    10561 ttcaaacaat atcaaacgat ttatgctggt ttcaatccag cggctgaaag ctatacttat
    10621 aatgactatt tccaaattgg tatgattggg atgattgtaa gcttagttgt ggtactaacg
    10681 gtcgctaata tatcaatgaa caaaaagaaa cgctatgcaa tggcagcaaa cgtaccagct
    10741 gaaggtggcg acgcaccaat gatctcatgg ttagccgtat tattaccagt tttaggggtc
    10801 gttcttttag atcttcaaat catcttaggt tttattctag caggtatctg ggcattgctg
    10861 tttactggaa aattgcgcgg aggttataaa gaaatctgtc gtcagtttgc gaaattattt
    10921 acagatggcg cagtcgatgt tgcaccaatg gttggtttct tgatgacctt agcgatgttc
    10981 aataattcag ctgcgtatgc ttctccttat ttttcagcta tttttggaga ttggataccg
    11041 caatcaccat tagtcttggc aatcgtattt gctatattga caccactagg atttttccgc
    11101 ggtccaatga atctagtcgg ttcaggctca gctattttag ctgtcgtgtt agctgttaat
    11161 ccaacgatgt cgccagcctt tttgttcccg ttatttgcaa ttacaacgat tgctcctcag
    11221 catttagata ttacacaatc ttgggttgct tggggattag gttatacaaa agtaacttct
    11281 cgagaatata tgaagaaatc catcccaact ggttggatca tcggtgcgat cttatgcctg
    11341 atcacgttct tattgtacgg gaacgcctag taaacaaaac gtcgataaaa acgattagat
    11401 aaatggagga aaagaaaatg aaacgttttg tgcgaatggg aattgatgtt ggcggtacac
    11461 atacgaaagc agtcgcaatc gataatgcga cgcacgaaat catcggaaaa tcttcagtga
    11521 aaacaaccca cgatgatgtc cgtggagtag ccgcaggtgt cgttcagtct tttcaaaatt
    11581 gtttgcggga aaataatatc agtccagaag atgttgtgtt tgttgcacat tctaccacac
    11641 aagcgactaa tgcactgatt gaaggagatg tagcaaaagt cggcgtaatc gggatggcaa
    11701 aaggtggatt agaaggtttt ttagcaaaac gacaaactag attgaacgat atcgatttag
    11761 ggaataagaa aaaaatagag attgtcaatg cattcttacc tgtcaaaaac ttaaatgtag
    11821 accgtgtttc agaaacgatt tcttctttag aaagagaaag agcggaggtc ttggtttctt
    11881 ctatggcatt tggagtagat aatggggaac cagaacgagt tgtttatgaa gcagcatctg
    11941 tgaaagcgat ccctacaacg atggcttctg acatcacaaa attatatgga ttgacaagaa
    12001 gaactcggac agcagcaatc aacgcatcta ttttaccaaa aatgctagat acggcaacct
    12061 cgacagaaga ctccgtacga gaagcagggg tcaatgtttc tttgatgatt atgcgcggag
    12121 acggcggagt aatggaaatc aatgaaatga aaaaacgacc tgttttaacc atgctttccg
    12181 gtcctgctgc atcagtcatg ggctctttga tgtacttgag agcgtctaat ggtgtttatt
    12241 ttgaagtagg cggaacaaca accaatatcg gggtcattaa aaatggacgt ccagcaatcg
    12301 attattcaat tgtaggtggc cacccaacat atatctcttc attagatgtt cgggttctag
    12361 gtgtggcagg cggttcgatg gtacgggcta atcagtcagg aatcatcgat gtcggtccac
    12421 gttctgcaca tattgccggt ctagattatg cagtatttac ggagacagaa aaaatcaaag
    12481 gtcctaaggt tgaatttttc tctcctaaag aaggagaccc tgctgattac gtcaaggtag
    12541 tgatggaaga cggagaagaa gtgactatta caaatacatg tgcagccaat gttcttggat
    12601 tagtgcaaga agaacatttt tcttatggaa atgtcccatc tgcacgaaaa gccatacagg
    12661 cattagcaga ctattgtcat acgacggttg aagacatcgc agagcagatc atggaaaagt
    12721 cctatgctaa gattgaaccg gtgattttag aattggcaga caagtatcac ttggagaaag
    12781 atcagatttc tcttgtaggt gtagggggcg gcgctgcctc attgattact tactttagta
    12841 ataaaatggg tgtgaaatat tcgattcctg aaaacgccga agtgatttct tctattggtg
    12901 tagcacttgc aatggtccga gatgttgtag aacgtatcat cccttcgcca agtaaagaag
    12961 atatacgatc gttgaaaaat gaagcgatga ataaagcgat tgaatcaggc gctacaccag
    13021 aatcgatcga agtccacgta gaaatcgatc cacatacttc aaaagtaacg gctatcgcta
    13081 ctggttctac ggaagtgaaa gcaaccgatc taaccaagga gatcacaaca gaagaagcac
    13141 tagagcttgc tgcagaggat atgcgattaa ataaaaatga agtttgctta ttggaaaata
    13201 cacctttctt ttatgtatgc ggcgaacaga atcgttcaaa aaatgctgga agtcttcgga
    13261 tcatcgatca aaaaggattt atcaaggtcc aacgtggaca tgcttcatgt atgaaaacaa
    13321 ctgcagccaa ttatatgaca gctgtcgaac aactttggga agatatggca gtttatcaaa
    13381 ctgaattgat tgcccgacca gaattttatc tctgcttagg agcaagagtc tctgatttta
    13441 cagcgacgga tctggaacaa ctacagcttt tgatggatct ggaagtatct actatggaac
    13501 cggaggaaga agtgatcgtc gttgccggaa atatcaaaca aacataagtt agaagagtta
    13561 ctaaacgaat tacaacagat ccccgaagaa atttggggat tctaccagtt tcggcgagat
    13621 ttattttgga aaaagattcc tttgtccaag cagaaaattc ttatacaaca atcaatcgat
    13681 tgtggtatcg aaacggcatg tagcatcaaa aaaaagtacc cttttgccga tgtaggagaa
    13741 atctatgaac aaatggctgt tccaatcgtt tcctgtgaat cagagcaaat aaacgaacga
    13801 atcacttttg caacttatgc agaagatgaa gggatccgat tgatgacgga gccgttagaa
    13861 aaattaaaat gttcaggact agcttcgatc tctaaagaaa cagcgcaagc tttgattatt
    13921 ggacatgaac tatttcacca catcgaagct tctgttaagg gaatttatac gcaaagcgaa
    13981 aaaattgtgc tgtggcggtt gcctttttat acacatcaat caaacattcg tgcattgagc
    14041 gagatagcag cgatgagttt ttcaaaagag atgaatcaaa gcgatttttc gccttatgta
    14101 ttagaggcag tcttactttg gccatataat gaaattcgta gccaagggat cttagaagaa
    14161 ataaaagaaa tagaaaaaag atgtacggag tatgatttag attacaaata aagagactgt
    14221 gacaaaagtc gtgtcacagt cctttttttc cgaataaacg gtgttctcct gtcaactcct
    14281 cggtcacaag tcaaaatatt caacaaacat gagaagctgt tttttgaaat tgttccttgt
    14341 gactcggaga tgaacggcag gctcacgacc tcttggaaaa aggtgttcaa aagcttgcat
    14401 ctgcggtaat aagaacagcc aagttaaaaa cataagaagc tatttttact tggctgttct
    14461 tattacttga tgcagggcag ctttttcaca acctctcaat aaacggtgtg caatcagctg
    14521 accctcggta ttagctcaaa aaaaacggca aaatatgagg aactattttg ccgtttattt
    14581 ctctaatact caggtcaaaa cgctgattcc aaaacctctt atttgatggg tctgtcctga
    14641 atatcttcaa aataatcggg gtaattatga atcacgtatt cttgctcctc gataatcata
    14701 tgtaaatgca tcgtgcaaag aaatccgact tcatcaaagt tatgatcaat cattgattga
    14761 agcattgtct gatgttcatc taatactcga ccccagtcta attctgaaat cgtcaaacgt
    14821 aaccatctga aacggttcaa gtgcgtacta taactttcta gccaatccca aatttctcgt
    14881 tttccagcaa tttcataaca agttcggtga aaatcagtat ccaaatcaaa aaaagctttt
    14941 ttattttgag cgatcactgc ctgatcggtt tgttctagta ttttttctag tttagcttga
    15001 tcttgcttgg tcattttcgc actgcattct tgcaggatcg gtttttccaa cttttcccgc
    15061 gtataacggg ctaatgaagc agatcggata tcgatttttg aaacataagt gccggactgt
    15121 ggaatggttt ttacaagctc ttgattgcgc agttggatca gtgcttctcg aatcggtgta
    15181 cgcccgatag ctagttgttc ttctaacact ttatcggaga ttttacttcc aggtgctaac
    15241 tcggaataaa tgatttgttt acgtatagat tcataagctt gctgttgcaa attattgata
    15301 accatttaac cttcctttct atgcagatat aatctattat aacataaatc gcttacaaat
    15361 cgtgtgggca taaaaacaca gtctgcaaaa aaagccctga taggcatgcc tattagagcc
    15421 tcgaaaagaa aaatattgaa tcaatctggt cgtacctctt ggatcggttt gacacgaccg
    15481 ccgattcttt tagcaaaagc ttcggattct tttttgtctt tgaaaatgag cagattgtct
    15541 aagttttttt cccgagtgcg acagccagtt ttatcgatgt aaccgtgcat tactttaacg
    15601 acatacatgg ggatcacctg cttttttctt ttcattatac gaactactcg acacaataca
    15661 agaaaaatgc ttagacggta tttattaaga atttacatca tccaaagaag cgtacgcttg
    15721 gaataactgt tctaactccg gattgacttt gctcagcttc actaatcgtt gcgaatcaga
    15781 aagtaaaaaa cagtgtttac tactgccagt agtttttaat tttttgtctt cagttcccac
    15841 aatttcgtag ctaaaatcca accgtgttcc cgtatacttt tggatagttg ggtggatgct
    15901 cactttttct ccgtaatgga tcatttcttt atagctgcaa tttacttcca gaacggggat
    15961 gatgatccca gattcttcga tttttttata gggaaaccct agatgtacca ataaggctac
    16021 ccgagcctct tcaaaccagc ggatataatt gctgtgatga ataatcccca tttgatctgt
    16081 ttcataataa aaaggttctc gcacataacc tgtaaatttc tccatgactc atgcctcgtt
    16141 ttccattgat tttttacaaa aataattcat ccacttcgga agtgcttcgc tgatttgtgt
    16201 cggcaaaaca atatattgtt cttttgccag ctggtctcca atgaaactat gaagataaac
    16261 agctgctgca atcgtttggt cattttgagg aaactgggct aaaaatcctg tgatcatccc
    16321 agctaaagtg tcacccatgc cgcctgttgc catagcagca gaacctaaag gattggtgta
    16381 gaagtgctgg tgaccataaa tttttgtctc atgacttttc aaaacgagca cagcaccgat
    16441 ttcctgttgc ttctttttgt tttctttatc ccgttgttct tgtatcggta gagaactcaa
    16501 ccgttgccac tccatttgat gaggtgtaag tacagtttgt tcgggatagg gtagcaaaag
    16561 atgattagca acaagtaaac ttaaagcaga gccatcaatc accagccatt gttcttttga
    16621 ttgtttttct agtacttgtt tgaaacgttc caaactctta gaagaagtac ccaatccggg
    16681 accaatcaag agtacatctg cttgctttat tacggaaaga aactggtcag tatccgtcca
    16741 atctacaatc attgcttctg gcagatgact gtgaagagca ctccgatttt tttcatcagt
    16801 tactacagtc gtgagacccg ctccactgtg tacggcagct aaagcactca taatgattgc
    16861 tccgccatat tgttcattgc cgccaatcaa aacgatacgt ccaaatgttc ctttatgtga
    16921 aatctttgga cgaggtgtga tgactctttt cactagttgt tcgtttatca gcatcgaaaa
    16981 acctcctttt actcattata gcgtatattt tttttagaga aaaacagata agaaaaggaa
    17041 ttgtcagatt ttttgaacta tttttttctg taatttgtgg tatcatgaac acgaagactt
    17101 aaataagtgg aggattaact ttatgacaat tgattggcaa aaagaagtag aagcacgtaa
    17161 agaagacttg cttgaagact tgaaaaatct tttacgtgtc aacagcgagc gcgatgattc
    17221 aaaagtaaca ccagatgcac catttggacc tggacctcgc gacgcattga aacatatgtt
    17281 agcttatggc gaacgtgatg gatttaaagt aaaaaatgtc gataactatg cagggcatat
    17341 tgatcttggc gaaggagacg aaacgctagg gatatttgga cacatggatg tcgttcctgc
    17401 aggagatggc tgggacacag atccttatga accagtaatc aaggatggaa agatctttgc
    17461 acgcggatca agcgatgaca aaggtccaag tatggcagct tactatgcga tgaagatcat
    17521 taaagaactt gacttgaaac tatcaaaaaa agttcgcttc gtagtcggca gcgatgaaga
    17581 aagcggctgg ggcgatatgg cttattattt cgaacatgaa gaagaaccag attttggttt
    17641 ctcaccagat gctgaattcc caatcatcaa tggtgaaaaa ggaaatgttt ctttagcttt
    17701 acgcttcaaa ggcgacaatg caggagatta tgtcttgaaa tcttttgttt ccggacttcg
    17761 cgaaaatatg gtgccaggaa cagcaactgc agcacttcaa gtaccaagtg cagatgcagc
    17821 tatcgcgatg gaagaagcat tttaccaatt catcgaagca aatccagtaa gcggaacgat
    17881 tgaagcggac aatacgtatg taaaaatcga attagttggt aaaggcgcac atggtgcaag
    17941 cccacaatct ggtatcaatg caggttcttt cttggcattg ttcttagata actatgaatt
    18001 cctaggatct gctaaacaat ttatccatgt agcagctgct tatgtccatg aagattttta
    18061 tggagaaaaa ctaggagtcg cttatgaaga tgaaaaaatg ggcaaattga caatgaatgc
    18121 tggactattt gcttttgaag aaaatggtac agaggaagcg aacttcatca atatgaattt
    18181 ccgttttcca aaaggtgtaa cagtagacgg attgcaaagt gacatcgaac aaacagtggg
    18241 gcaagaaggc gcaacagtta ctcgaggtgc tcgcgtaatg gaaccacatt acgtaccaat
    18301 ggatgatcca ttggtggcta cattattgca agtctatgaa gaccacactg gagaaaaagg
    18361 ctacgaacaa atcatcggtg gcggaactta cggacgtttg ttgaaacgtg gcgttgctta
    18421 tggagcgatg ttcccaggat atactgatac aatgcatcaa gcaaacgaat ttatgagtct
    18481 tgatgacttg ttcagagcaa cagcaatcta tgcagatgcc atctatcgtt tggcgaaata
    18541 aaaatactaa aaaactccgg ctatctctga cagctggagt ttttatgttt aaaaatgaat
    18601 tagtacatcg gtattcttaa aagtaattta gttcgtcttt tgatttctca ggagggatca
    18661 aaaaggcgaa agcactaagt atagcaaaac ttgtgatgat ttgcgtacga tacgtaaacc
    18721 aataatgaat ctgtgagtga ttagctaaaa tattgtacca aatatacggc gttacagcag
    18781 ctaaaacaaa caaaactaga agttggaacg gacgccagct atttcgttta cgataagcaa
    18841 aatagagaaa aacaagacaa gtaatcaata gcaataatat tgctactttt ggatacatca
    18901 atctaatatt gcttttcagc atctctaatc gatgcaacgg gtatttgtcg tttccttccg
    18961 ttcgaaagag gatttggttg attgcatctt ttacaatgtt tttgttcaat atgatcgatg
    19021 ataggatcca tttagccacc catgtcaaac catagcctaa tccccaaaag ataccggtaa
    19081 aaacaaaact gcctacttgt ttgctcaatg aactgcgttt atccattaaa tacttatttg
    19141 ttacataata aataatgatc acaggtatcc cccaagagat gaccggtgtt gtcaacaagt
    19201 caaaaaagtt agctaaagaa ccaatgacaa agaaatacaa gaataattgt tggttggtgt
    19261 tggagagttg tggttttgtt aaaaatagta atacagacaa taaagtcaaa atccaaatat
    19321 ttgaaaattg catactgaag aaaaaggttg cagcatttcc tatagcaagc gaaatcaaca
    19381 aggctgtcgt taaatagatg tcgctacact tggttaaaaa gtagctcgtc aatccaatca
    19441 gcagaaaaag aatggctgcg taaatcattc gaatactccc caaactcatc cctagcagta
    19501 aaggtcgtaa aaaaagctga taaccatgcc aataacgggg ataatccgcc atagacatcg
    19561 cgttctccaa ggagttcaat tcaggatctg cttttgtttt tcgtatcatc aaatgatcag
    19621 taaaattatc taaccgagtg cctagcatat ttccttgatt gatagaagga tacattcctt
    19681 ctttttcaac catctgtaga gatttttctg cattcgattc aatagagtgc tgcggaatga
    19741 aaaaagtaat tgtcagtagt acgcaatagc tagcgatcaa gagaacaaaa gcaaaaaata
    19801 tctttttaaa tagtttcatg agtatctcct atatctgttt tcattttttc tcttttatct
    19861 agtaaaaaag gagagaggat ataaacaggt agacctaatg ctaaaacaaa cacataccgt
    19921 aaactaaaag ccacaggagt agcaatcata atagtagcaa aaacaagcat accgggaaga
    19981 aaaaggagta aataccgata ttttttctta aagattgcta aaagtgcact gccgatagct
    20041 aaccacagta atgtgccgga accaaagaaa tcacgagcat cgatgatttt tttcattcct
    20101 gacaatccag taagatgttc aatcaaatct acttgcttca taccataatt attcgtgttt
    20161 acacgagtat ctaaaaagcc gtaactattt tttgtaccag gtacccagaa cccgaacgtt
    20221 tctaaaatat aagcatctgt atagattttt atattacttg gtaaatttct tgcccatagt
    20281 gatagaaatt cttttttatg tgtattcaaa aactcattgt taaaattttt atgccacttg
    20341 attgagtctg caagactagg agaatacgct tcataatctt ttatcgggag tagctgattc
    20401 atgaatgctt tatctttctt agacaagtca ccatccataa caatagttct agaaatttgc
    20461 tgcaaaggaa cacctaattg ttcagcagtt tctgtaggaa tagcataacg agccatcaac
    20521 gggtggatga gtagtccagc aatcactgta taacccagca ccacttgaaa gaatgcttga
    20581 tacctttttt gtttcaaaat aatcaagccg caaatcaaac ctgtgccggt tactacataa
    20641 atcccattat ttcttagata ggtggtcagt aaagcaagga tgattacgta aatctgattg
    20701 agaatcgttg gcttttctcg ttgaaaaata aagtcaaaaa ccgctaagga aaataaaaag
    20761 caagcgatag agaacaaagt atctttttgc atgttcaagg cgtagattgg aaaaaccggt
    20821 gcaaatgcaa aatagcataa actaatgagt accggaaaaa aatggatgtt tctttttaac
    20881 agccagcaaa gagaataact tatcgctgaa gctgttatca atgtttgaaa aatcgaaaat
    20941 aaaaatacac cgtgattgat cgtatcagtt ggtaaatcat gaacaaatag cttgaccagt
    21001 aaggtgaata agactggatg atgattgctt ataggaacat cacccataga ttgggcgacc
    21061 gaacttaacg aatcaggcaa aaccactcca ggatacaatg ccaacagata aggaagccaa
    21121 cacagaacta aaaagacagt aaagaataaa aaatattttc ttgaagagaa agcctgtgtg
    21181 ttttgagcag taaaaaatgt ttccagtttt ggcattgata aataaatcga ttgtaaaaat
    21241 aaaaaagaat acacaaaaat ggagaaataa acaagaaagt aaaaaatact tccatcagca
    21301 atcgttgtct ttgtatagtt tgctgagaca tactgaaatt gtatctgtct gccaatagaa
    21361 agtgtaagag caaaagctgc agaaaaaaac aatcgggcaa tagctgcaac tttgtttttt
    21421 attgaaaaca actctattcc atattgctta aaagcaagta gcaaaaccat ggctaaacca
    21481 atgattactg ttggattcaa agttcctaga ccataagaag taaaaaaagc aaaacctgac
    21541 gcaaataaag ttgcaaaaaa agtaggaaat agattctcct ttttcttatg cataaaaaag
    21601 gcctcctact tcatttcttt attttgatat tcgtggtaga catggtataa attcaattca
    21661 tattctttct tggcatttgt gacgaccgta tccaaaatca accctgttac taatgaaaga
    21721 agggctaaag tcatcaatcc agtacttaaa atcgcagaag ggatgcgtgt aataaagctt
    21781 gtcagcatat attcacgaat cacaggaatc cccatgacca atccaaataa aaagaagaag
    21841 atggtccaga tcccaaagaa aagcaaaggt ttataatctt tgcacatttt gatgatcatc
    21901 atgatcactt tgaatccgtc agagaatgta ttcaattttg attcgctacc ttcaggacga
    21961 tcgcgataat cgattggaat ctcaacgaac ttgaattttt tatctaaagc atgaatcgtc
    22021 aattctgttt cgatttgaaa accagaactc ataataggga agcttttgac aaaaattcga
    22081 ttgaatcctc gatagccggt catgacatcg cgaatttttg ttttatataa gtaagtaatt
    22141 gtatttttaa ccagattatt cccaaaatca tggaaaggac gcttattttc atcaaaatag
    22201 gttccatttg atagacgatc gccaatgacc atatctgctt cgccagaacg taatttctca
    22261 agcaaaccat gtgcagcttc tgcaggataa gtatcatcac catctaccat caaataataa
    22321 tcggcatcaa tatcaaaaaa catttgacgg attacattcc cttttccttg acgtggttct
    22381 ttctttacga ttgctccgcc ttgaacggcc aattcgtatg ttcgatcggt cgaattatta
    22441 tcatagacat aaatatctgc ttctggtaat tcccttttaa aatcttcaat aacagttgaa
    22501 attgtcgcct cttcgttata acaaggaatg agcacggcaa ttttttcacg tgattccatt
    22561 tgtcactctc cttatataat aaaactaatc ttatttcaat aataccgtat aataactaca
    22621 aatactagct attccaagag gaaatagccc tatttttata aaaatcaccc actataaaag
    22681 aaggatagga tacctctctt ttagatagtg ggtgcgtatt cgttatttca tttttttgac
    22741 gtattaatct attgcgccct tttttcttga aatgtctaat acggttcctg tatgagcatc
    22801 tgctaaaaat tcataataaa cagtttcgcc atcttctatc cgggaaagcc caccccgata
    22861 agcactcgac cgaacggcga atttccgaat aggttctttt tcaaaagaga tccaagcacc
    22921 ttcgattgcg ccttctttta aaaaagatgt tttgatattt tcaagcactt ggtctgctga
    22981 taacactttt ttgcgatgat accaaatcgt tgaagctacc ccactcaata atcctagacc
    23041 gatcccaacg gctaacccgc ctttaaaata gcttaattcg ctttcttcat acatgtggat
    23101 atacctcctt cttctcactt gctactcttt gtgtttattt taccacactc tcatattttc
    23161 tttgaaataa ataaccgcta ccagaaaaaa taagctataa taaatacgat cggtctgcag
    23221 aaaaaatcat attttatccg cagatcaata taagtctgag aggagtttag tctgaaattt
    23281 agacgaatag catatggaag aaaaaacatt tcaacgaatc aaagagttaa cagaattaca
    23341 aggaacaagc ggttttgaac aagacgttcg cgcctatatg gaaagtcata tggaaccgct
    23401 ggtagatgag ttacagttag atggtttggg cggcattttt gggctgcgcc accatgaaga
    23461 agcggatgca cctcgtgtaa tggtcgcagc acatatggat gaagtcggat tcatgctgac
    23521 acaaatccaa gaaaacgggc tttttaaagt cgtacctcta ggcggatgga acccttacgt
    23581 tgtttctgct caacgtttta cacttaaaac ctctactggt aagaattatc catgtatttc
    23641 ttcttccgtt cccccacatt tattacgagg aacaagcgga caaaaatcag tagaagtaac
    23701 agatattttg ttcgatgctg ggtttgaatc aagagaagag gcaatgagtt acggtgttct
    23761 tccaggagac acaatcgttc cttacgctga aacgatcaaa acagccaacg ggaagaatat
    23821 catcagcaaa tcgtgggaca accgctatgg ctgtacaatg gtattagaag ctttagaagc
    23881 gttacaaaat gaaacattag gtcatacatt gattgcaggc gcgaatgtcc aagaagaagt
    23941 aggcttgcgt ggttctaaag cttctgtcaa taaattcaag ccagatctgt tctttgcagt
    24001 agactgctct gcagcagatg acacagtgac gaaaaatggc acattcggac atttaggaga
    24061 aggtacattg atgcgtatcc aagatccagg tttgatcatg cttcctcgtt tgcgtgagta
    24121 cttgctagat atcgctgaaa caaacaacat cccttatcaa tactttgttt caaaaggcgg
    24181 tacagatgca ggtgctgctc atacacaaaa tgaaggaatc ccaagtacag tgattggcgt
    24241 agtgggacgt tatatccata cgcatcaaac gatgttcagt attcgcgatt ttgaagctgc
    24301 acgagaaatg ttgattcaaa cattgaaagg tttagataaa tcaacagtga acacgatcgt
    24361 atacgggaaa taacgcgata gtgaaactat aaaatctggc aaaagcaacc ttttgtcaga
    24421 ttttttttat aaaaagagga tcaatgtttg ttagttcgaa agaaaggtgt ataatttaat
    24481 tgaaaaatac ataaacagaa agtagaggaa aaagaatgat tatcccaaat agtttagaag
    24541 aattagcagg ctacgtagaa actggaaaaa gtgtattttt ctttactgcc ggctggtgcg
    24601 gagactgccg ttttatcaaa ccgcaaatgc ctgaaatcga aaatgacttt tctgattggc
    24661 aatttattga agtagaccgt gacaagtaca tagatgtagc cgctgaatgg aacatttttg
    24721 gtatcccaag ttttgtcgtg atccaagacg gaaaagaatt aggtcgtcta gtgaataaag
    24781 atcgtaaaac aaaacaggaa atcgaatcgt tcctagaaag tgtaataaaa ggctgacatc
    24841 tgttaggagg ggtcaagatg aatcaagaat ataagaacat tttagtaggt attgacggta
    24901 gtgaacaagc acttgctgct ttcaaaaaag cgttagaagt ggctcgccgt aataatggga
    24961 aagtatatgt agcaaacgta atcgatcagc aattttataa ttttatgggc tatgctccta
    25021 ttgatcaaaa tctagtcgat cagcaaactg aaacagccaa gcaaatgatc gaagaatgta
    25081 aagaatatgg aaaatctgtt gattacacaa atatagaagg aattattgca tacggctctg
    25141 ttcgtgaatc catggcagtc aaattgccaa aaaaatatgc aatcgatcta atcatggttg
    25201 ggcagtctgg actaaatgca gtcgaacgct ttatgacagg aagtgtagcc agctacgtca
    25261 tacgtcattc accttgtgat gtcttgatcg tttctgacga agaagaacag ccaagtaatt
    25321 aagcaagtgg aacttgtata acctttttaa tcaaacagta aagggactgt gacaaaagtc
    25381 ttgtcacagt cccttgttac gaataaatgg tgttccagaa gaagcttctt cggaaataaa
    25441 gcatgaaaaa aatctcatgc agtgaatgca attttttgtt aagataaatc tcagcaaagg
    25501 ataatggagg aaagaacatg atttttgctt ataacaaagc acacgtcgga gacactttga
    25561 tggttatcat agccgacgat aaaggaacag aaaacactgt cgaacgtaaa tggaatgttg
    25621 ctcggatcaa agatgaacaa ggtcaagttg ttgcttggaa ctttttccat atctccgatc
    25681 atctaacaat tgaagggaac ggccaagtaa gcttaacaga tgaagaatta gcgaaattga
    25741 atcgattgat taaagaagca ggattcgaag aacagcttga agctgatcat gaaccgaaga
    25801 tcgttgtcgg gtatgtaaaa acatgcgtcc cgcatcctga ctctgaccat ttatcgatta
    25861 ctgagacaga agtggacaat gggacagtat tgcaaattgt ttgcggtgca ccaaatatcg
    25921 aagctggaca aaaagttgtc gttgcaaaac caggagccat gatgccagat ggaatgatga
    25981 tctggccagg aactttgcga ggggtagcca gctatggaat gatttgttct gctcgtgaat
    26041 tgcatttgcc aaatgctcca gaaaaaaaag gaatcttagt attgcctgag gatgccgtag
    26101 tcggagaagt atttccaaaa taaagaggtt gtgacaagtc tttgtcacaa cctctttatt
    26161 attaattttc actattttga ttttgtttta atgccgattg atcgatcgtc aattcaacag
    26221 tagttgtttt ttctttcgag ccttcatagt aagtgatttt gacagaatct cccacttttt
    26281 tcttatacaa agcagattga agttctacac cagaagaaac ttctttgtca tcgatcttag
    26341 taatgacgtc gtattgtttc aacccagctt tttctgccgg tgtagctgtt tggacatttg
    26401 tcacgatgac accgttagtt acagatgaag gaatcttcaa gatttgttct tgttgctgag
    26461 tagaaacggc agataagtcg accattgtga tacctagagc tggacgtgtc acttttccgt
    26521 ctttttccag ttggttgatg atattcacta catcgttact tgggatggca aatcccattc
    26581 cttctacgct tacattagaa gtcgattcag aagtacttgc gatcttactt gagttgatac
    26641 cgataacttg tccttcgata ttcactaaag gaccaccaga atttcccggg ttgatcgctg
    26701 catctgtttg gatcgcattg atattgactg tttcgttcga ttcattcgta cttgttactt
    26761 gtcggttcaa ggaagaaatg attcctgaag tgacagaatt cgcatattca gatcctaatg
    26821 gggaaccaat cgcaatcgca ggttcaccaa ccttcaaagc acttgagtcc ccaaaagagg
    26881 caaccgtttc gactttgtct gaattgatct tcaacaccgc taaatctgaa taggcatctg
    26941 ttccaactaa ttcagctttt accttcgtac cgtctttcat catgacttcc aaaccttgct
    27001 gtccatctac tacgtgattg tttgtgacta cataagcagt attcccgtct tttttgtaga
    27061 ttacgccgct tccttcacta taagcttcca aattactatc atctgagctg ctactttctt
    27121 cttgctgccc gaataatccg ccaaaaccgc ttgattggtt ttgactttgt aagttgataa
    27181 cagaaacgac agcgtcttga actttatcta ccgctttggt gatatcagaa tctacattga
    27241 ctttgacatt ttccacaact gtttcaccgg cagaattttg attgccgctt gtagcagtag
    27301 aactattgtt gccagtgccc atcgctgcat aaaaaatgcc agctgtcagt agtcctccaa
    27361 cgatccctcc aacaagcccc agacccaact tacgccagag gctattattt ttattttttt
    27421 tcatctttgg tgtcacattt tttctatcca tgtgttcctc ctccaattac attcatttat
    27481 ctactgttag tatagtctta tttgttgact ttagtctact ttgagacttt gaacttttta
    27541 tgaaaaactt taggaaaaat cattgttttc agatgaaaat atttgatttt ctttttccac
    27601 agatattgtg taaaactacg cggataggat tggaaaaata agggataaaa catatccaca
    27661 gcctgtgtat aaagtggata aattcttata aaacgtatgt ttgcctaatg aatgtgatga
    27721 aataaggtaa aacaacgctg ttttctgtgg ataaacctgt ggatgttgtg cataactgtt
    27781 ggggaaatct ttttaacaaa aatagtcaaa ataaacatat agacgaaaaa attgattata
    27841 ataaacaaag tgagcgtgaa gatcaaagga gagaaaacat gaatatcaaa attatttcag
    27901 taggaaaatt aaaagaaaaa tacttgatcc aaggtatcaa tgagtatgtc aaacggctga
    27961 atgcctatgc gaaaatcgaa ttgatcgaag ttcctgacga aaaagccccg gagaatttaa
    28021 gcgaagcgca aatgagacaa gtaaaagaaa aagaaggaga acggatctta gcgaaaatca
    28081 aagaacaaga atacgtctat ggcttagcaa ttgaagggaa aaatcccaca agcgaatctt
    28141 ttgctaaaca aatcgaccaa ttaggtatcc aaggaaaaag ccaccttgtt tttgttatcg
    28201 gcggttcttt aggactcagc gaagccgtca tgaaaagaag caatgctcag atctcttttg
    28261 gcaaaatgac gtatccacat caattgatgc gcctgatcct cgtcgagcaa atctaccgtg
    28321 ccttccggat caacgcgggg gctccatacc acaaataatg atatttttta agagaaaaat
    28381 agcataaaaa tctagttatc cgcataaaaa ctggacttat cacactttat caaggtcaaa
    28441 accactcaat ttactactaa tttactactt atgaatgagc tttgatacga cgatttatcc
    28501 ttgaaaagtg aagatataaa gatacttcca ataaaatttg aatatttaat aggtagacac
    28561 ttcaaaaaat gaggtgtcta tttttttacc cgattttgaa aggaagtgaa cttatgaaaa
    28621 caaaaaatca agaatcaaaa ggtcgttccc cactctttaa gaccatcaaa cattcattca
    28681 gccaataaaa aagaaaggat aggtaaaaat atggaactta aatttgtgat tcccaacatg
    28741 gaaaaaacat tcggcaattt agaatttgct ggcgaggata aagtcgttca gcgaagaatc
    28801 aacggacggc taactgtctt atcaagaagc tataatctct attctgatgt tcaaagagca
    28861 gatgatattg tggtggtgct tcctgctgaa gctggcgaaa aacatttcgg ctttgaggaa
    28921 cgtgtgaagt tagtcaatcc acgtattacc gcagagggct acaaaatcgg cactcgtggt
    28981 tttacaaatt accttttaca tgctgacgac atgataaaag aataaagaaa gagaggaaaa
    29041 atgatgagat tagcaaatgg cattgtatta gataaagaca cgacttttgg agaattgaaa
    29101 ttctctgctc tacgtcgtga agtgagaatc caaaatgaag acgggtcggt ttcagatgaa
    29161 atcaaggaac gtacctatga cttaaaatcc aaaggacaag gacgcatgat tcaagtaagt
    29221 attcctgcca gcgtgccttt gaaagagttt gattataacg cacgggtgga acttatcaat
    29281 cccattgcgg acaccgttgc tactgccacc tatcaaggag cagatgttga ctggtatatc
    29341 aaggcagacg atattgtgct gacaaaggat tctagttcat tcaaagctca accacaagca
    29401 aagaaagaac cgacacaaga caaatagtcg ctaggtagaa aggagacttt ttcgcatgaa
    29461 acagcgtggt aaaaggattc gcccatctgg taaagattta gtctttcatt ttacgatagc
    29521 gtcactcctg cctgttttcc tgctggttgt cggactgttt catgtgaaga caatccagca
    29581 gatcaactgg caggatttta acctatcaca agcagataag attgacattc cctatttaat
    29641 tatcagtttc agtgtcgcaa ttcttatctg cttgctggta gcgtttgtat tcaaacgggt
    29701 tcgctatgat acggttaaac aactttacca ccgtcaaaaa ctggcaaaga tgatacttga
    29761 aaacaagtgg tatgaatctg aacaggtcaa aacagagggt ttctttaaag atagtgctgg
    29821 tcgtacaaag gaaaagataa cctacttccc taaaatgtat tatcgactta aaaatggctt
    29881 gatacagata cgggtggaaa tcacgctggg aaaatatcaa gaccaactct tacacttgga
    29941 aaagaaatta gagagtggct tgtactgtga gctgacggat aaagagttaa aggattccta
    30001 tgtggaatat actttgctct atgacaccat agccagtcgt atttctattg atgaagtaga
    30061 agctaaagat ggtaaacttc gcttaatgaa aaacgtatgg tgggaatatg ataagctccc
    30121 tcatatgttg attgctggtg gtacaggtgg cggtaaaact tactttatac tgacactgat
    30181 tgaagccttg cttcatacag attcaaaact gtatattctt gacccgaaaa atgctgacct
    30241 tgcggactta ggttctgtga tggcaaatgt ctactataga aaagaagact tgctttcttg
    30301 cattgaaaca ttctatgaag aaatgatgaa acgtagtgag gaaatgaagc agatgaagaa
    30361 ctataagact ggcaaaaatt atgcttactt aggtctcccg gcacacttct taatctttga
    30421 tgaatacgtc gctttcatgg aaatgctggg aacaaaagaa aacaccgcag ttatgaataa
    30481 gctgaaacag attgtcatgt taggtcgtca agctggcttc tttctaatac tggcttgtca
    30541 acgtccagac gcaaaatatt taggcgacgg aatccgtgat ca
//
LOCUS       DACEQH010000032        30463 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.032, whole genome shotgun sequence.
ACCESSION   DACEQH010000032 DACEQH010000000
VERSION     DACEQH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.032"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..552)
                     /locus_tag="IW833_001523"
     CDS             complement(<1..552)
                     /locus_tag="IW833_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ1307649.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(678..2606)
                     /locus_tag="IW833_001524"
     CDS             complement(678..2606)
                     /locus_tag="IW833_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1307650.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(2912..3628)
                     /locus_tag="IW833_001525"
     CDS             complement(2912..3628)
                     /locus_tag="IW833_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1307651.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3629..5452)
                     /gene="glpO"
                     /locus_tag="IW833_001526"
     CDS             complement(3629..5452)
                     /gene="glpO"
                     /locus_tag="IW833_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ1307652.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5466..6962)
                     /gene="glpK"
                     /locus_tag="IW833_001527"
     CDS             complement(5466..6962)
                     /gene="glpK"
                     /locus_tag="IW833_001527"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ1307653.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7098..8558)
                     /locus_tag="IW833_001528"
     CDS             complement(7098..8558)
                     /locus_tag="IW833_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1307654.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSSFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8548..9843)
                     /locus_tag="IW833_001529"
     CDS             complement(8548..9843)
                     /locus_tag="IW833_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1307655.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10175..10852)
                     /locus_tag="IW833_001530"
     CDS             complement(10175..10852)
                     /locus_tag="IW833_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ1307656.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(10882..11973)
                     /locus_tag="IW833_001531"
     CDS             complement(10882..11973)
                     /locus_tag="IW833_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ1307657.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12074..12508)
                     /locus_tag="IW833_001532"
     CDS             complement(12074..12508)
                     /locus_tag="IW833_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1307658.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13092..14549)
                     /gene="ascB"
                     /locus_tag="IW833_001533"
     CDS             complement(13092..14549)
                     /gene="ascB"
                     /locus_tag="IW833_001533"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1307659.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14546..16441)
                     /locus_tag="IW833_001534"
     CDS             complement(14546..16441)
                     /locus_tag="IW833_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1307660.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16499..18274)
                     /locus_tag="IW833_001535"
     CDS             complement(16499..18274)
                     /locus_tag="IW833_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1307661.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18324..19820)
                     /locus_tag="IW833_001536"
     CDS             complement(18324..19820)
                     /locus_tag="IW833_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1307662.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20014..21636)
                     /locus_tag="IW833_001537"
     CDS             complement(20014..21636)
                     /locus_tag="IW833_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1307663.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(21964..23277)
                     /locus_tag="IW833_001538"
     CDS             complement(21964..23277)
                     /locus_tag="IW833_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1307664.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(23522..23746)
                     /locus_tag="IW833_001539"
     CDS             complement(23522..23746)
                     /locus_tag="IW833_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307665.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(23766..24134)
                     /locus_tag="IW833_001540"
     CDS             complement(23766..24134)
                     /locus_tag="IW833_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1307666.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24330..25040)
                     /locus_tag="IW833_001541"
     CDS             complement(24330..25040)
                     /locus_tag="IW833_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307667.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25058..25555)
                     /locus_tag="IW833_001542"
     CDS             complement(25058..25555)
                     /locus_tag="IW833_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1307668.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(25592..26044)
                     /locus_tag="IW833_001543"
     CDS             complement(25592..26044)
                     /locus_tag="IW833_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1307669.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26179..26784)
                     /locus_tag="IW833_001544"
     CDS             complement(26179..26784)
                     /locus_tag="IW833_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1307670.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27234..27854)
                     /locus_tag="IW833_001545"
     CDS             complement(27234..27854)
                     /locus_tag="IW833_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1307671.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(27856..28725)
                     /locus_tag="IW833_001546"
     CDS             complement(27856..28725)
                     /locus_tag="IW833_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ1307672.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(28748..29155)
                     /locus_tag="IW833_001547"
     CDS             complement(28748..29155)
                     /locus_tag="IW833_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1307673.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29174..30463)
                     /locus_tag="IW833_001548"
     CDS             complement(29174..30463)
                     /locus_tag="IW833_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1307674.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         8630 a         6626 c         5021 g        10186 t
ORIGIN      
        1 ttttctttga aacatctttc ggtactcgac agcatacaag acaaaagatt gtaaaagacc
       61 gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt ggtaattctg ggaaaagcag
      121 caaaaggatc atgccaacat agatgacagc agtcgctact ttgccatacc acttcgctcc
      181 acttaatttg atatttttag atagcccgat taatccagct acaaacataa agccttcttt
      241 tatcaagaat agaccaaaca acagccaaag ccacggtcga taccatgcca tagacaaaat
      301 cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc accttcccta agtctgttac
      361 ctgattatat ctccgagcaa tgaccccgtc tagataatca gacaaacctg agacaacaat
      421 gaccgctcct gctatataaa atgactgtct cagcacaaaa taaagataaa tcggcagcaa
      481 aaaaatacgg aatatcgata aaagattcgg aatcgtttgt atttctttac gccagtctgt
      541 gcttaatttc attttatcgc cctacctcct cagcactctt ttctttttat tttagcttac
      601 acggggaata aaaccaatga tttcactgtt tttatttttc tttacagcca ctgatattat
      661 gagctaattt gagataatca cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt
      721 ttgcgcattt cttttactag tactttatga gctttttttg atcctcgctc taggtcacgc
      781 tgccgtttac gtgttttata atcgataaac agactgtcta aatcgtaacc tgtcgggtac
      841 agatcttctg ctgaacgcac cagacgaaca cgctgacgag gcacgtggat tatttttttg
      901 tctaaatata catcaatcgt atcttcacct atatcttcat aaactaacgc ttctttttgg
      961 tattcattga caaatatccg atcacctttt gcaaagagct ggcgttcttt tttattctcc
     1021 ttcctatttg taactgctgt cttaggaaga aattcttttt tctgtgtccg ataggctttt
     1081 tccgccaagt aacgctctgc ttgatggatc aatttcatcg acatatccat tttatgagca
     1141 atccacaaag cttggctttc tcctgtttct ccaacccgca actgatattt aggacgaagt
     1201 gcttcccgat cgaaagccat agcagccggc acaaaatctt catgatctcc ggcgaatttc
     1261 ttgatttctc cataatgggt cgtggcaatg atcaatgccc cttgctcata catagattcc
     1321 atgattgcga tcgctagtgc agccccttca tttggctctg ttccactgcc aatctcatct
     1381 aaaagtacca acgtattcct ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga
     1441 ccagaaaatg tactcaacgc attttctaaa ttttggtggt caccgacatc gacgaaaatc
     1501 tcatcaaaga cagcaagctc tgtcccttct tttgccggaa cttgcatacc gaacatcgcc
     1561 atcaaagtca ataaaccgac tgtcttcaac acgactgttt tgcctccggc atttgctccg
     1621 gtgataatca agcctcgata gtctttaccg atttcaacat ccaatggtac cgcatgctca
     1681 ggtaaaaatg gatgtctgcc ttgcttgata atgatatgtt ccgatttatt gattctaggt
     1741 gtgcttccat tgatttctcg gctaaatttt gcccgagcaa agatgatatc taacaccgtg
     1801 atcgtttcga tcaataaatc gatcacttct tcgttttctg cgatagcacc agtcaatgcc
     1861 gccaggactt gatattcttc actgatttct tcagctttta acaactgata gtgttcattc
     1921 aatttagaaa tcacagttgg ttcaataaaa accgtcgttc ttttatttga ttcatcgata
     1981 atcgttccat ctattttatt tttatagctg gctttgatag gaatcgtata gtattcgcct
     2041 ttttgaacaa tcattgcttc ttggatcatt tctttgtttt tcggatgacg caagaatttc
     2101 agtaattttg actggatttc tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa
     2161 ttacgtgacg cgtcgtcact gactttatta ttttttattt tctggtcaat caactcttct
     2221 acatttatca aatcaggaag atgtttgcta taagcaaaca acaagggtgt ctggtattga
     2281 ttcttatcaa aaaactttgt gatcatccga ctgcttctaa gaaaatccgc atattcgatc
     2341 aaatcggttg gctgcagaat caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc
     2401 ggcaacccca taaatggaac atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt
     2461 tcttcttgac gatccaagac agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt
     2521 tttttgctat agtttccaat tgctcttgct tggatttcct ttttgatccg atcaaattgt
     2581 aattgactaa tcatttcgtt gtccatattc tctccttttc ttgaagagat acctgtccca
     2641 aaataaataa gggcctagag ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc
     2701 ctatccttat tagaatacaa agatattgtt ttcctgacag gtaccgtttc ataagcacac
     2761 caaataaact ttgtttattg gctattcgac ttactgatta gataccagct acactcacaa
     2821 aacatacccc attcttctag gtcttattta ttttccactt aactatagca agacacattt
     2881 tggttgtcaa actttagtat gttttataga tttacaccat taatgaatag acacctaccg
     2941 cgataattgc tccgatcatt ggacctgcga ttggtaccca tgaatatgcc caatctgaag
     3001 ttcctttatt agctattggc aataccgcat gtgctaatcg aggaccaaag tcccgagcag
     3061 ggttgattgc atatcctgtt ggccctccta atgaaagacc gatggctaag atcaatccac
     3121 ctacagcgaa tacgttcgtt ccaggtgcaa attcgttttg tccaaaagct agcaacccaa
     3181 gaactaatac aaatgtacca attaattctg tgatcacatt tgctggataa ttccgtatag
     3241 caggacctgt tgcaaacgtt cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg
     3301 gcaaataaga aagccaaaca agaatggccc ctgcaaaagc tcctaaaact tgcgcaataa
     3361 tgaaaggaac gacaagattc caactaattg cccctgtcat cgccatagct agagagactg
     3421 caggattcaa atgagctggg cccatgaatc cagaaatata gacagccatt gttacagcga
     3481 gtccccaacc aaatgcgatg acaatccagc cagaagcctg tgctttactt ttgtttaaat
     3541 tcacagccgc acacacacca tctccaagca aaactaggat cagtgtccca ataaactccc
     3601 ccatgatttg agtcatatca ctgttcattt atttctcccc ctttagttcc cgcagatctg
     3661 attctgcaat caatgcttct aaacgtttag tctgttgcgc cttctcctct tctgtccatt
     3721 caaaatatcc agcaattgca tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt
     3781 caaacaacag atgatttgta cgacgaatga gataatcgcc tggggccaaa accatttctt
     3841 cttctaaacc ataacgtaac cgagctgact cagcgagact caaaccagga taaggtgcca
     3901 tttcttttgc taattcaaag atacgtcggg cattagtccc gtaaaaatca gcgatataag
     3961 tagcatcttc ttcctctaaa ccggctgcta ctccttcttt tgctaattct gtcactgttt
     4021 cttctaattt agttggatcg aagtttccac ctgaaatctg atatttttta gagtcgattt
     4081 ctttcgtctc tattccgtat tcttctttta ataattggcg tatcaatcgc aaagctcctt
     4141 ctgccatctt acggtaatcg gtgattttcc caccagataa agtcagcaaa ccatctggtt
     4201 ctctttctaa agaactacct ctggaaatcg tagaaggtgc tttttcatca cggctgtttt
     4261 ccaaatggtt caacacgtct tctacttcag cacgagaaac tttgttttcc ttatattcac
     4321 ttactgtatc gacaacttta ttgaagctct tgtctgaaat cgatccatta tctccaccat
     4381 tgtaatcaga accagaatta ccaatcaaca gtggacgaag tcctgcccag cttgcttcga
     4441 tatcattcaa tgtgatattt gcttctggat agcgatgatt gatcacatcc aatagataat
     4501 ctacatcttc ttgtgtgact ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg
     4561 taccaaagta agtcttgttt tctcttggaa tagcaaaaac catccgccca tcttgctttc
     4621 ctgtatcgaa gtatgtgggt tgcggtacag gcagtttttt cgcatctaca actaaatgga
     4681 tccctttagt tggacgcatt ttaggagaga ctggacgcgt aaaattcaag tttcgtactt
     4741 tatctaccca aggaccactc gtattgatca ctaattttgc tttgatttcg atcacttcat
     4801 ctgtcagcag gtcacgagct ttgacgccaa cgatttgatc gccatcatat aaaaagccaa
     4861 ccgctttcat tttacttact agataagccc catcttcggc agcctttttg atattatcaa
     4921 tcactaaacg ggcatcattg ttgcggaaat ctagatagac acccgcgcct tttagccctt
     4981 cttttttcaa aaacggttct ctttccaata cttcttctgg tgtcagcgta taattctcat
     5041 atttggttcc tgtcacattt gccagtttgt cataaaggtc cattgctact ttgaccgaga
     5101 acatattgaa ggttgttgct ccttcgccct catagattgg caaaagcatt ggatctggtt
     5161 ttgggatgtg tggggcaata ctttgtacga ctgcacgttc accaactgtg tcagctacta
     5221 cttccacatc aaatgtcttc agataacgaa taccgccatg aacaagtttg gtcgagcgag
     5281 aggaagtccc ttctgcaaaa tcctgcattt cgatcaatcc tgttttgatt cctgatgctg
     5341 ctgactggac tgccacaccg gcaccagtga ttccgccacc gatgatcaac agatccaatt
     5401 cttcttgctg cattttttga atgctatctt gtcttgtctt gtttgaaaac atatggattt
     5461 cctccttatt caaatgcttg ggtagctttt actgcttttt tccaaccgtt gtaaagtttt
     5521 tctcggcgct cttcctccat ttgaacttca aatagcttgc cagcttcata aaattcgcgg
     5581 atctcgtctg tatctttcca ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc
     5641 gccgttgttt ccaaattttc tgctcgttgg atcggtacat tcaagatatc tgtttggaat
     5701 tgcatcagat attcattgtt tgctgcaccg ccatctactt tcaatacagg aatcttgatt
     5761 ccagtgtcat cttgcatcgt atcgatgata tctctcactt gataagcaat cgactgtaaa
     5821 gtagctttaa tgatatcttc ttttgtcgta ccacgtgtca agccaaacat agcccctcgt
     5881 gctgcctgat cccaataagg agcacctagc ccaacgaatg caggaacaac ataaacctca
     5941 tcttcgcttg tagagcgttt cgctgcgtct tctgaatcgc tagctttttg caacatttga
     6001 agtccatctc tcagccattg aatcgaagaa ccagccacaa aaatacttcc ttctaaagca
     6061 tagtaaacct ttccatttat gccgtaacca attgtcgtca ataaattatt tttggataat
     6121 tgcggttttt caccggtatt catgacaata aatgaacctg taccataagt attcttgacc
     6181 atgcctggtt cgaatgccat ttgtccgaat aatgcggctt gctggtctcc tgccatccct
     6241 gaaattggtg cttcgcttcc ataaaaatga taaccttgcg tataaccata aacttctgag
     6301 ttagaagtga cttttggtaa catcactcga ggaatattta gtaaatctaa aatctcctga
     6361 tcccaatcta gctgatggat attgaacaac atcgtccgac tggcattcga gtaatcagta
     6421 acatgtgctt gtccatcggt cagtttccat actaaccaag aatcgatcgt tccaaacatc
     6481 aattcgcctt tttcagctcg ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc
     6541 ttcgttgctg agaaataggc atcgatcact aaccctgttt tttcgtggaa aaattcttga
     6601 tagccttcat tttttaagtt atctgcaatt ccagctgact gtctggattg ccaaacaact
     6661 gcatgataga ttggacgtcc tgtatctttt tcccatatta ctgttgtttc acgctggttc
     6721 gttatgccaa tcccagcaat ctgatttggt ttgatccctg attcgatgaa cgctccggca
     6781 ataactgact gtacagaatt ccagatttca tttgcatcat gttccaccca actttcttgc
     6841 gggaaatatt gcgtgaactc tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag
     6901 ataattgctc gtgagctagt tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc
     6961 attgtgattc cctccattat atgcaaacgc tttctttaac tactcttatc atactactgt
     7021 tttaaaaaaa gaaactgtca aaattttatt ctttttttct ttttttgaca gttttttatt
     7081 atgaagctat tttttaatca attcttttct tttttcagcg tctctattct tcttttcaat
     7141 cgaataatgt cgtaaggtga cgcaaattct gacagataat attcacactg ttttttgttt
     7201 ctctggcaaa atgtgatcac caaatcatat tctttacctt cttcataact tccaatctca
     7261 ataccgccga ttcccctcag ttctctgatc agatattgtt gaaaagcaat acgataaatc
     7321 ggcagacttt ctaaatcgat tccaatcact actggttttg caatataaaa atcgatcatc
     7381 aaaagaacat tggcatactg gatcatcagg taactaagct cactatcttc agctcttttc
     7441 ctcggcaatt gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc
     7501 ccgagcattt tctgctgctg ttccaaaagc cgatcacgct catagacttc gatctttcca
     7561 acaaagaaaa accattcatt gttgatctgg gaaatttggt agcctactgc cttttcttct
     7621 ttgattttca aacgacgagg acgataatgc cataacatcg tttcccgaat gtaagtatct
     7681 aaaacagcgg ttggtaactt tttgctccgt gtcaaatcat actgataaaa tatttcttcg
     7741 tctactatcg aaaaagaaac aaaagagctg taaaacatca ttgtttcgta tacattcagt
     7801 tctgctgctg ttcgactaag atgcatcacg atgattggat cgattgcttg ataaagccgg
     7861 tccgactgat agagtaattt cttttctttc aaagttgcgt acgtactttt ttcattcaat
     7921 agtctttttt tcgtaatccc gagccagcaa gcaatctgtg cttctgctga tgccgtaaac
     7981 gttgtttcta atacacggtc caatcctaga atgaacggtc ttttttctgg tgtattttgc
     8041 aaaaaaagcg gtcgctgatc ttccgggata tattggaata gttcataata aaaatatcgt
     8101 atctgtagct cttctccgta taattgaccg tttttgattt gtaatccaaa ttcctctaag
     8161 agctggttca attctttgat ctttcgaaaa acagaagact cactgatatt aaacgctgtc
     8221 gctaatcgaa cgatggatac ttgctgatgt tctaataaat agacgagcat cttaaacttc
     8281 aagctttcct gcaataaaaa caatagaatt ttttccaaac ttacatcttc gtccaacaca
     8341 agcagcgcct gatactcatt tcgctgtact tctacttttt tttccatcat tcggccaatc
     8401 aattcaatat cttccaaata ttgatcaaac gaggcttttg acagttggac atgctctctc
     8461 aaaccattga tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat caggatctgt
     8521 aactgctctt ttctttctaa taatgcttca atcttcatag gcagtattcg ccacctttga
     8581 ccacaccata tctggcacac ggtattctgg ctggaaaaat agctcatgtt cttctaactg
     8641 ttcaaacgtc catccttctt ttactgcaat tgtcagttgg tcaagaaggt ggaacatctc
     8701 ttttttgctt tggagctgtg ctccaagaat ctttttttca gaatcaaata gaaatttgag
     8761 tttaactttg ctttgattta ttgctgtaag cttgtagtct acgtatcctg tttcaatttt
     8821 ttggggataa aaaatgctct cttcttctgt caaacctacg cttcctaaga aatagccaaa
     8881 ccactgattg ccgactggac gataggttcc ctgatccttt gtctgtgagc cactaattgt
     8941 cttgctgaca tttcgtgctg tacggacagc gttatttact agagaaacat acattttttc
     9001 atttgttgga cgaaaagtta cttggatcgc atcgccaatc gcataaacgt ctttttgaga
     9061 cgtttgcaag tagtcatcta cccagatcgt tccatcatca ttcaatgtta attgttcttc
     9121 ccacatctga ttgtcggggc gagaatgatt agccagcaaa acaagatcat agttctcctt
     9181 tcttttttcc gtctctacaa tagcggtttc attttcggtc aaagacacaa cttgttcttc
     9241 taaaaaaagt gaaagccctt gatttttcat ttcctttact aacggttctg tcatttcagg
     9301 atctagataa cgaaaaagaa ttgactttcg gctttcgtaa agatggattt gtttttttct
     9361 tgatgccaac ccttcagcta tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt
     9421 ctttgcattc atgatctttt gctgaagagc cgttacgttt tgtatctttt taactgggtg
     9481 aataagcgtt gattctgcag aaatatttcg aaacttttga tttgaaccag tcgctagaat
     9541 caaacgatca aaatccaatt tttttcctcc tgctaaaacg actttttttt ctttcaatcc
     9601 tattactgtc ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt cttctttcgt
     9661 gatccaagaa agttgttttt cactggtaac ctttcctttt aatatcaaat tgaacccgct
     9721 gggaatccat cctaacttcg attgcttttc aatcaaatat atttcacttg ttggcatcaa
     9781 ttggcgaaga taaatagccg catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt
     9841 catctgcatt cctctttctt ataaggtgtt ctactgctaa cttctcaatc agaaaacaca
     9901 gtattcataa ctcgctttca accttatgat acatagcgat gcagcagaat caaccctctc
     9961 catcataccg tgttctaggt ctgcttctca ataatgtact agtggaccta attgaagagt
    10021 atcatttctg aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa actcgtcttt
    10081 tacgtacctc tgtccaaata ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt
    10141 aatgtaatag ttagaataga cacatcattt atttttaatt ctttaatgct tcaacaactg
    10201 cttcgcctaa ttgatgagca gattgaggat tttgccctgt aagaagccgc ccatcaattt
    10261 gaaccacttc tgaaaaagct gctccagact gatagtttgc tcctttttct ttcaaagcat
    10321 cttctgctaa gaatggtact tcattcgttg taccatttgc ttcctcttct tcattactga
    10381 acccagtcac tgtacgtcca gctaaaatag attttccaga ctcatctgta actggcaaca
    10441 atcctacaac accgtgacaa acagctgaga ccagcccttc atttcgatag atttgtgaag
    10501 cgactttccc aagatctatt gcttctggaa aatcccacat tgttccgtgg ccaccagcat
    10561 aataaaccgc tgcataatca tttgcatcca catcatcagg acgtaaggta tttcccaagg
    10621 cccgatttct gaaatcttca tcttcataat aggcccaatc gatttcatcc atattcaaaa
    10681 cactttgcgg ttctaaaggt acatatccgc ctttaggact gacaaaatct gcatcgatcc
    10741 cattttttac taatacatca taaaaatgag tcagttcact taaccacaaa cctgtaggac
    10801 gctcgatctt ttcgtacttt tctacatttg ttaatacgat caatactttc attttcccat
    10861 cctctttcta taaaaattgc gttattgata acgtgctttc aagaaattat ataatgggct
    10921 tggcaaataa agctctttat atgcttcttc aaaaggtttc gttacaaatt catcctcacg
    10981 gttttccaat aatttctctg cccagtcagg atcgacgatc atttgctgtc caattgctgc
    11041 aagttctgca tatttcaata cctgctctac atcttcgcga ttgcggatcc caccgacacc
    11101 aactaaaggg atctttcctt gaatcgtgtt atggaccact tcaagaatcg atttatcgtt
    11161 gtatttatca gaacgagcta ttcgatcata catatttaat gaaacatgca gataatctag
    11221 ttttgactca cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga tacctggtgt
    11281 ttcatattct tctggtgaga aacgataccc tacgatgaat ggttttttag catattgttc
    11341 aaccgctgag aaaaccgttg ttaataattc ttcaataaaa cgataacgat tttccaagct
    11401 accgccccat tgatcctctc gtctattcga atgcggagag aaaaattgtt ggatcaaata
    11461 cgtatttgct ccatgcaact cgattccatc aaatcctgat tcaatcgctc tacgtgtggc
    11521 tttgccaaat gcttggattg tttcaacgat ttcttcttca ctcattgctc gtggtgtttc
    11581 cgcatcagga cgttttgctg caactgcact tgcagataca ggctgttctc cagataaagt
    11641 tgctcgactt gtcattcgtc caccgtggaa aatctgaata atcgcttttg cgccttctcc
    11701 ttggattgct ttcgctagct cacttaatcg aggaatcatt tcatcatgag cgatgctgag
    11761 ttcacctggc cagccttttc cgatatcttg gacatttgca gcaccagtaa taacagcacc
    11821 aagaccttta gaacgttgtt tataataagt gatctcatct tcggtgatca ctccattgaa
    11881 aaagctgagt tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt gaactcctga
    11941 aggtaaagta attgattgat caaattgtga cattgtcatt cctcccataa ttcgctctga
    12001 ttaacctatc gagaatcatc tatcattaaa ccactcgatt agttgtacat gcaactaatt
    12061 tgcttaaaaa attttatttt ttttgtaact tatctgttgc atcattcatt gtctgggcta
    12121 attctttact tgcttcattt cccaaaactg ctttgtatga ctgatgaagt tcttcccagc
    12181 taccatcgac ttccttagca aattggcgtc cttgttctgt aagataaact aatgacaagc
    12241 gaccttcgct aactgttttg acgtatcctt tatattctaa tttttcaaca aaacgagcaa
    12301 tcgttgatgg ggcggaatag agcatttgtg caatatcttt ttgtggtatt ccatcttttt
    12361 cttttaatat caataaaata aaaccataag ttggtgccag acctgtcctt ctaaacgctt
    12421 catcagctaa tttattcaac atgcgatcta gctttttcac gctaaagaaa agacattcat
    12481 ctaacgcttt ttcaattggc ttttccattt catcccctcc attggcgtac atatcatagt
    12541 atgattttta ttttttgtca aaaaacacgg aataatgtcc gcctcctctg tctagatttg
    12601 aaaaaaagaa gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg
    12661 aacaccgtga atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt
    12721 gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgaaaa actggctttt
    12781 gaacaccgtt tatatcagag gttgtgaaaa agccgtcctg catcaagtaa taagaacagc
    12841 caaacaaaaa tagttcctca tattttgtcg ttttttgagc taatgccgat ggtcagctga
    12901 ttgtacaccg tttattgaga ggtcgtgagc ctgccgttga actccgagtc acaaggagca
    12961 atttcaaaaa acagcttctc atgttttatg actatggtga cttgtgatcg aggagttggc
    13021 aggagaatac tgtttattcg taaagaaggg gctgtgacaa gacttttgtc acagtccctt
    13081 atttcctttt attataggat ttcgccattt gattggatga cttgtctgta ccaatcaaat
    13141 gattttttct ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc aacatagata
    13201 tatccataac gttttgacat ttgtgaagta gaagcgctga cgatatcaat gcagccccac
    13261 gacgtataac caaataaatc tactccatct ttgatagctt ccttcatctg ctcaatatgc
    13321 tgcttcaaat agtcaatgcg gtaatcgtct tgaacggttc catcttcttc tactttgtct
    13381 atagctccga gtccgttttc agctatgaac agtggttttt gatacctatc atataattga
    13441 ttcaaagcaa ttctcaaacc cttaggatca atctgccagc cccattcact tgatggaaga
    13501 tacggatttt taattcctcc agttattaag tttccttgga ccccttccca attttctgga
    13561 tgagcactga ctgcactaga catgtagtag ctaaaagaaa caaaatcaac agggtaggct
    13621 gctaagattt cttcatcacc tacttctatt tttaagttga cacctttttc ttcaaacatt
    13681 cgttgcgtat acgctggata atatcctctt gcttgaacat cactaaagaa caaagaagtt
    13741 ctttgctgct caattgtttt ttgaatatca tccggatcac aagaatacgg aacaagaagc
    13801 tgatagctga tcatacaacc gacttgtgaa cctttgataa tttcatgaca agcttttgta
    13861 gctaaagcac ttgctataaa ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg
    13921 ggatggtttt cttcaattac tcctacgcta acaaaagggt gatgttttac acagttgatc
    13981 tcattgaaag tcatccaata ttttacttta tttttatagc gtttgaatac tgtttcagcg
    14041 aaacgaacgt aaaactgaat aagctttcga ttaggccatc caccatattc tgtaaccaaa
    14101 tataacggca tttcataatg ggaaagagaa acgactggct caatcccata ttttagacat
    14161 tcgtcaaaca cttgatcata aaaaaataat cccgcttcat tgggtgtttc ttcatcacca
    14221 tgaggaaaaa ttcttgtcca tgcgatagac atccgatagc atttaaaacc catttcagca
    14281 aataaacgga tgtcttcttt aaagtgatga tagaaatcat ttccgtgtct tttaggataa
    14341 taatacttgc tgttgggatc ctgcgccgct tttacttgct gatgcgtcat catatgccat
    14401 tgatctgtcc atttttcttt aggtaaatcg ctgtcgaatg tatacacatc agacacagat
    14461 aatccttttt catctacatc ccatgcacct tcacattgat tggccgctgt cgctccgccc
    14521 cataaaaatc cttctggaaa ttttttcata tcgatccccc taaacttgct agttttcttg
    14581 tttgattatt tttcttatga ctagtaatga tatttttgaa tctactacta tttgtaacaa
    14641 taaccattgt ggtcggatca tatccctctt tgattaattc ttgcaaatct acttccatca
    14701 ataaatcgcc tttacgtact ttttttccat cagatactgc actttgaaag tatttgccat
    14761 ttaaattcac tgtatcaata ccgatatgga tcagtatctc tgctccttct tcactgacta
    14821 ccccaattgc atgtttcgtt ggaaaaactg ttgaaacttc cccgtttaca gaactgtaga
    14881 tttttccgtc cgttggaatg atcgcaaacc cttctcctaa agatccacta gcaaatgttg
    14941 gatcgtttac ttcagataaa tcaattattt cacctgctgc aggagtactt aacactatat
    15001 cctttgagga ttcttcttcg atttcatcct tgtagccgaa aagaaaagtt agcatacagg
    15061 aactgatcat cgctaagaaa taactagcaa ctagccaaac aaatccttca ccaatataaa
    15121 tcgggaaaga caaaatactt cttgtagcaa ctgccaaagc accggctcct gcggcaccag
    15181 ctactccacc aaatatagct ccacatatac aagcaatgta aaatggtcgt ttatatttaa
    15241 gagtcacacc ataaacagca ggttcagtga ttccaaataa tgctgtgata aatgcagata
    15301 aagataaact ttttactttc ttatttttcg ttttcaaaaa taccccaagt actgctcctg
    15361 cttgcgcagc tacggcagtc atacatgctg gacctagcgt atcacgaccg tagaaagaaa
    15421 gattgttggt cattactggt acaagtcccc aatgtaaccc aaaaatcact aacacttgcc
    15481 aaaatccgcc gataacagca ccagataaaa cagggctaaa gttataaaca gctaaatatc
    15541 cagctgcaat cagctgactt gcataatctg caattggacc tacaactaat aaaataattg
    15601 ggaccatcac gatgatacaa atgaatggtg tcaataaatt tcgaatactt tcgtgcaagc
    15661 ttttgcgaat cttaggctca atataagaaa gaacccatac agctaaaatt gctggaataa
    15721 ccgtacttgt ataccttgct agaatcacgg gcatccctag aaatcgcaag gttatgctat
    15781 cactatatgc actgacaatc gtaggataaa cgagagctgc tgcaataacc atagcgatga
    15841 aacgatctgt attgaacttt tttgcagcag tatatgccaa aaatataggc aagaaataga
    15901 aaaatgcatc agctgctgca ttcaaaatga tataagttcc tgattcagta gttaataaat
    15961 tcaaactcgt acataaggcg agtactcctt tgatcaaacc acttcctgcc aagacgccta
    16021 aagtcggggc aaaaacagcg gcgatcatgt ctataaaaga gtttaaaatg ccttttttct
    16081 cagttcctct ctttccacta gatgcatcat cttctagttt catttgtttg gataatacgt
    16141 catacacttc atttactgca tttcctacta cgacttgaaa cattcctcca gcttcgacta
    16201 cagtgatcac accttctagt tgctctacct tctccttatc tgccttctta ttatctttta
    16261 gggtaaagcg taaccttgtt gcacaatggt aaacattgct gacattttct tcgcctccga
    16321 caaatcggac aatatctttc gctaattgtg aatagtccat ttttacttcc cccttgttaa
    16381 ttaatgccgt agcaatcctt cttacaagat aagtatagtt cttagaaaac ggtttctcca
    16441 ttcaattagt accccttcta atagcgaacg aaactgtaca atagtgtgta tggtttactt
    16501 agccgagcaa atcactcact gcttgaaaga aaacaagtat atcgtttctc ttatagtcaa
    16561 aggagacaaa aaaaacacgc ttgatcttcc tttgctgatg tataagtggt atctcaagct
    16621 gatactctgt gatttttgtt tctacttggc ttttgataag acagcacaac aaagtctcgg
    16681 catttaacgg aagcataact aggtcagaac taggagagat tagttgctga atggaatcta
    16741 tctttttatg gatttttatt tgattttctt ctttgtagta atgatcaagc agttttgttt
    16801 tctttttttc ttcaactatg gtcagctttt cacgcaaaat ctgataaatc tcccaagatt
    16861 caaactcatc tagaacacta ggtagataaa tcaaatcatc ccactcaaaa ataagctctt
    16921 gctcagtagt caaaattgca tcatattccg ttattttgtt tgattgattt ttgaaaagat
    16981 acactggttc tatccggcat tctttgatcc acatgttcat actttgttga atctgttttt
    17041 ttagtgtatt caaaatacta attggctggt tactaacaat cagtatctcc ccttgttttc
    17101 gaaaaggatt caacgcttcg gcaagataca aaacaaaata agcgctttca gctaaattgg
    17161 gacgtaattg aaaatgtatt gggaagaaac gccgaattaa ataaatttct aaaggataac
    17221 gggagatgat cttttgtaca aaatgattca tggtattatg tccggcatcc atcctcgttt
    17281 tcatttgttt gatatgggtt agcaaaaatt ctgtttcatg gaacaataac gaaacaggaa
    17341 gatctaaaaa acgaataatc gtttgctcaa atgctcttag attcttctct atttctttgt
    17401 ttttttcatc attaatcatc aaataattta actctaatag tcgattttca attgcttgtt
    17461 tttcttgatt ggaaaaagaa tagggtagtt ctgatgataa cttttttgtg agggtctcta
    17521 ccatcggaat aaataatgta tacctgtttg ttttttctaa aagttcttct cttcgaagaa
    17581 tggtcaaaac gagataccga gaaaatcgta tgaagtcctc tcctgaaaca atataattat
    17641 atgatataag aacctctttt aatgtctttt ggacagtcgg catgatattg gaaaaaattg
    17701 tactgatttg ttgaggaaac cttgcgtgtt ggatcaccgt ttctgttcca acaagagata
    17761 agaaaattct ttttgaattt tcttcagcaa tgatttttag tccttttgtc gacgaaactt
    17821 ccagttctaa tttaggattg cgctcgatcc aacgcaaaat cgtttttatt tgtaatgata
    17881 cggctgtttt tgaaagaaag aaaatgtctg ctatcttttg catactgaca tacccttttt
    17941 gaaacaataa gtacgtcaca atagttgaag ctcggaaatc catgctggaa tagattttat
    18001 gtttcgtcac ctcttgtatg atttgctgtt tagtttcaga ttcaattgat tctaaaaaat
    18061 aacctgaacg tttatttgac tggataatga ttttcttctc gtagatagca agcatatttt
    18121 tgatttcagt ttggatcgtt ttcttgttgg tacctaaagc ttcagctaat tcttcgcttg
    18181 ttatccaatg attcttattt cttaatacta caagaatttg gagttgtcgt ttatttaagt
    18241 ccatttgctt acacctcctc tttgttataa acatacagga aaaacctctc aaatgacaat
    18301 agtcacttgc agaggtttaa aaatcatttg aagaaagatc tatattcaag gtttttacca
    18361 ttagactgga taatttgttt ataatattca aaactatctt ttttcaatct cctaaagctt
    18421 ccgctcccat cattttgtct atccacataa ataaagccat atcgcttcct catttcccct
    18481 gttcctgccg atacgatatc gattggtccc caccataagt aaccaatgat ttggcaacca
    18541 tcttttattg cctcagcaat ttcctgaaga tgtattttta gatactcaca acgttttgga
    18601 tcgtggattc gattcaattc atctggtgct tcatctagtc caatgccatt ttcaactatg
    18661 aataatggta attcataacg gtctgtcaat tcattcatga cataacgaaa ccctagagga
    18721 tcaattggcc aactccaagg ttcgggagat ttttccttca aaaagggatt atcgaaacca
    18781 tttgctccgc cagtatctac gcgcatttct gcttcttttt tatagacagc gctacgataa
    18841 taactaaatg caatataatc tgccgtgtta gcttgcaagc actgttcgtc tccatctaac
    18901 attactggtg cacagtcttc ttccttccat gtcctgtaaa tataagctgg ataatgccct
    18961 cggcacatga catcggagaa aaaccatgaa ttgcgtttaa attgcagtga accaaataca
    19021 ttctctggat cacagtcata tgcataagtc gctaatgcag aaaagctaca cataataccc
    19081 atcttagcat ttggatcgat tttctttaat aattgagtag cacgggcgtt cgctagaaac
    19141 atatgatgac aggcttgata aatcactttt tttgttagtg tagcaatggg ttcaagttta
    19201 tctctagcag ggaatgtaac caatccagca gctgcataag gcatacgaaa agcattattg
    19261 atctcattaa aggtcatcca atatttcact ttcccttggt agcgttcaaa gacggtctgc
    19321 gcataaaatt caaaaaagtc gattaatttt cgattttgcc accccccata ctctcctacc
    19381 aatgctaagg gggtttcata atgactaaga gtaattactg gctcgatatc atatcgattt
    19441 aatgtattga ttagtctgtc ataaaatacg agaccagcct catttggctt ctgttcgtct
    19501 cctcttggaa atattcgtgc ccatgagatg cttgttcgaa aagcttttaa acctaactcc
    19561 ttcagtaaac gaatatcctc ttcaaatcga tgataaaaat caatcgcttc atgagaaaga
    19621 taatggagtg attcgtctaa agcaatctcc caaatctttt tttcttcatt ccactgaata
    19681 ctaggatgct ggttcatgat cccgataagc gtatctgtca cattaggtaa tttgccgtct
    19741 tccaaccatg caccttcaca ttgattagca gctactgctc cgccccataa aaaattttct
    19801 ggaaatattt cctgatccat aactatctct cctcgctatc aacaatttct tacttgaagt
    19861 atagctcgat tcatcaaaac aagatattct aactatttcg gcagaagaga agaaaaaaat
    19921 tgaatgctac tcttcatttt taatatttat atataacaaa tacataagac gatccccgaa
    19981 agaaaacact cgttaaccat ctaagcaaac gatttatttt acttccaaca caaatgcttc
    20041 ataaggtcgt aaatcaactt tgccgtctgg taactgtttc agaggataat ttgacacaat
    20101 aacccttaaa gcttcacgtg gaatagtgaa cgtctgcttt tcactggaca tattacaaat
    20161 aacgagccat tgctggtctt catatatgcg agaataggca aagacatgct caggcgtctt
    20221 ttccactaat tcatagtctc cccagacgat gatgtcatgt tttctacgca aatcgatcaa
    20281 tttcttgtac atataaaaaa cagaatcttg atctgctaac gcttcttctg cattgatttc
    20341 tttataattt ggattgactc gaagccatgg aattccagta gtaaacccag catgttcacg
    20401 ggcgttccat tgcataggcg tcctagcatt gtctcttcct ttcgccttga ttgattccaa
    20461 gatttcctct tttgtaaatc cagaagaaat cctttcgtta tacatattga ttgtctcaat
    20521 atcttttgct tccgcgatat catcgattgg gcaattcgtc atgcctaact cttctccttg
    20581 atagatataa ggggtgcctt tcatcatatg gagcaagata gcaaacatct tggcactttc
    20641 ttttcgatac acatcgtcat ttccccaacg agaaacaatt cgcggaagat cgtgattatt
    20701 ccaaaataaa ctattccatc cttcatcacc taaagatgtt tgccatttgg ataatacttt
    20761 tttcagttca gagatactca agggtttcaa atcccatttg tctttgcctt cttgctgatc
    20821 taatcctatg tgttcgaatt ggaagaccat ggataattca tttcttttag gattggaata
    20881 aagtttagcg atttcggtag ttgctcccca tgtttcgcca acagtcatca gatttttgtc
    20941 accaaaagtt gcttgattca tttcctgaag atactcatgc agtttaggtc cattacttgt
    21001 gatttcttta tccggttctt tgccaatcat atcgatcacg tccatacgaa agcccccaat
    21061 acctttatcg atccagaaat tcatcatttg ataaatctct tggcgaacct gtttattttt
    21121 ccaattcaaa tcaggttgac gcttgctata caagtgcaga aaatattgcc ccgttttttc
    21181 atccaactgc caagcagaac cactgaatat tgagcgcaat ctattaggct cttttccatt
    21241 gaccggatca cgccaaatat aatagtcccg ataaggattt tctttatttt tacgggcctc
    21301 aatgaaccac ggatgttcat cagaagtatg attcaccacc agatccatga tgatacggat
    21361 atttcttttc tttgcttctt caatcagttc ttccatttct tccatcgtac cgaattctgc
    21421 catgattgct tgataattac tgatatcata cccattgtca tcattcggag actcgtatac
    21481 tggagaaagc caaattgcat caatacctaa atacgccaga taatccaacc tggaaataat
    21541 cccccgcaaa tctccaatac catcttgatt tgaatcttga aaacttcttg gatagatttg
    21601 ataaacaact gaatggtgcc accatttttg ttccatgatt ttcctcctat aaatggatgt
    21661 ttctattttt tgcttaatca tttattttag tctctctcac taaaaattgt aacgctttca
    21721 aatagctatt gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc tgaggaaatt
    21781 ccctcaactg ttttttgaaa ggattgattt atgcagggaa gaatgctgat ttcacaactt
    21841 ctttataaac ggtgttcact cagctgtccc ttggtattag ctcaaaaaac ggcaaaatat
    21901 aagaaactat tttttcgttt ttttctctaa tactcaggcc agaacgctga tttcacaacc
    21961 tctttatttc acattcaata tccacatgaa tcccacaaat acaaagaaca gaccatacat
    22021 gattggagat acttcttttc ctcttttagc tgctaacatg gtaattggat agaagataaa
    22081 acctaatgca atcccatctg aaatactata agtcaaaggc atgccgatca agatcaagaa
    22141 tgaagggatc gcaatctcta agtttcccca ttcgatttgt cgcaaagatt gtgccatcaa
    22201 tactcctacc ataatcaatg ctggtgcagt tacttgtgaa gtaacaactg atagtaatgg
    22261 agaaaagaac aagcctaaca agaagaaaat ccctgtagta atagctgtga tacctgaacg
    22321 tcctccaacg gcgataccag ctgatgattc tacaaatgca ccaacaggag aagtaccaaa
    22381 taaggaaccc gcaagcatag ctgttgaatc agaagctaaa gctttcccta cacgaggcat
    22441 cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca actaaagttc ctgctgtatc
    22501 aaagaaagtc acaagcaaga atgtcagcac tacgacccac atttgaagag aattgatgtc
    22561 ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa cttggtgctg cggcaacaat
    22621 ttcagaaggc attggaatca aacctgtgac cagacctaaa atcgttgtag ctgccatccc
    22681 gatgaagatg ccacctggta cacgccgaac aagcaagata gcagttacga ctaatccaaa
    22741 aatcgtcaac caagtagaac caacgttgag tgatcctaat cctactaatg tagattcatt
    22801 cgaaacgatg atcccacctt cgctcagacc aaggaaagcg ataaatagac cgataccgcc
    22861 agaaatcgca tatttcaaat ctgctgggat cgcatcaatg atcatttccc gcagcttgaa
    22921 gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg ccggctaatg ctgtttccca
    22981 tggaatcccc attcctaaac aaacagaata agcaaaaaaa gcattgatcc caagagcagg
    23041 tgccgttcca attggataac gggctaatac ccccattaaa acacagccga tcgcactggc
    23101 taatgcagtc gcagtaaaca ctgctccttc atccattcca gaagcaccta atacagttgg
    23161 atttacaaat aagatataag ccattgagat aaatgttgta aatcctgcca gtacctcacg
    23221 ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa agtatttttt ccttcatctt
    23281 ccatccccct aaatccgaac attataaata aagtccgtta aaatttcgat ttattaataa
    23341 aacaacgtgt tttatcatat actaaaacaa aacataagta aacaaaaaat tcggatttta
    23401 agcaaaatcc gaacgttcca tctgattaaa tccctttatc tcaaagttca tgaacattca
    23461 ctttggatta caagaatttc tttattgtgt taataaagtt tcttcattct agtctcaaaa
    23521 atcaatagtg tgatttgatt ttaccatttc gtttctgttc ttctttgatg aattcttttg
    23581 tatcgacagg attcagatta tttagtccgc caaaacctgt atcgaccatt tttccccagc
    23641 cttttgtctt cttttttcct gatttgagat tctttgctcg aagcttctca ctatgttttg
    23701 ctcgccgata cttcaaataa ccttctaatc ttcccattag atccactgtt tttcctcctt
    23761 tttattcaat cagacgactc agcttttcct ataacaaaat aattgccttc agggtcagaa
    23821 aaattgaaag tcaagatgcc attgttatct gtgatttctc ctggttcttc catgttctcg
    23881 tagatagttt caaattcatc cgtaaaaaat actaaagaag gcggtggtcc taatacctct
    23941 ggtgaaaact tctgcacaaa tacttttggg aatacgccta attctaattc ttttgagatg
    24001 ctgagtacaa tactcttaaa ttcttctggc aattcgattg tttcagaaat ttctgcacca
    24061 attttttcta caaagaatcg gcaaatcatc tctacgtcat tgacataaag catcacacgt
    24121 gttttttgta tcatcatttg acccttcttt ctatttgatt ttctttcctg tctataaact
    24181 gattccatta tatcacgaaa gcgcaaacac cttgtgtttg atactattca agaaaaataa
    24241 agcaatagtt tctaggattg ctttcctcca caaacttttt ttacaaaaaa aaaacggcaa
    24301 tgaatgccgc tcttcttatt tcgattcttt tataattctg cttctgattc atctttggtt
    24361 agtgtgtgtc taacactagt tacgccaaat gtttgatcga ttttttcctt caacgcttcg
    24421 attgcctgtg cgccacgtgc cgcttgttca gcattgatgg attcaatgac cagcaccgct
    24481 gcttgtgttt cttccgtcag catcgttctt tgggcttcac gccagttgag acagcgacag
    24541 atcgctcctt gttcatcata gtagatgatt tcttccggaa gtgccggagc atcggaatct
    24601 gcacctaaag gcaggaatga ttcgccacct tgggcttttc ctaaatgcag atttcctgcg
    24661 atcttttcga tatcttctcc gccgcaaggt acgccaaaag acaaggaaat actattatag
    24721 atatctacaa gcggattgat tgggaaaaat tcccgttctt gatttgcacg tttcagtaaa
    24781 gcttcaatag aagaacgtgc acctttttta gtcttgaatg tggtaaatgc ctgacgccac
    24841 tgagcaatga cttcgttttg gctgaatgtt tcttctgtaa ggtaatttct tgcttctttt
    24901 gctgcctcat tcaataattg atgatacgtt tcttcacttt cttttgttac gtggttatcg
    24961 ataccattta cggataagat ttcgatggtt gcttcaggaa atacttccca gaaattggta
    25021 tccactatta cttttttcat ctgttcacac ctcttgatta gaataattgc ttgatgaata
    25081 gatagtctgt ttggggatca tctcccatat aaaaaacatg ttcgctgtat ttgataaatc
    25141 cttttttacg ataaaaagcg agcgcaacat cgttgtgttc ccaaacaccg agccaaatgg
    25201 ctgttttttg cttttctttt gctttttcaa tagctgtatc tagtaaaaat gtaccgatac
    25261 cttgatgttt aaattttttt cgtacataga ttcgctcgat ttctaaaaaa tgatcagcca
    25321 tttcctcaga ttgactcgtt cctgtattta gtttcaggta acccgcaggc tcttgatcga
    25381 tactcactag gaaaaaatcg gtttctttat cttttagctc ttctgtaagt ttttctagtg
    25441 cataagcttg tgtcaagtaa tcagctaaat gctccggtga attatctttt ccaaatgtat
    25501 ctgtaaatgt ttcgatacta atttgttgta gtaagtctac atctgaaggc gtcatttttc
    25561 gaatttcgta tttattcacg gcttcttcct ttcaatagat ccttttattg ccttttttga
    25621 caaaattcca atcttcgcta acattcttgc atactttatt taaaagatat tccaattgtt
    25681 ttgcttcttc gtcagaaaga ccatttaatg cgactgcatt ggagtagttg ttttctcgaa
    25741 tgatgaaagg atagatttct gctccttttt tagtcggata gagtttcttg atttttttat
    25801 tttctttatc ttccaaacgt tcgatcatcc cattggattc taatttcttg attgctcggg
    25861 cagtcgttgt acgatctact ttgatcattt ctgctagttt ttcttggatg attccaggat
    25921 tttcgcaaat acgtactaaa taaagatact gtcctcttgt taaatcgact tctttgaatt
    25981 cgatattgct aatagaatct agcgctcggg caatcattcc gatgtctcga aggatttcag
    26041 ccatttcatc ccacctttat tttgtttgta agactagttc acctcttttt gttgcaaatg
    26101 caactatttt caataaaaaa atcaagttct tttttaataa ctagtaaagt atattctttt
    26161 cttcttttac gaaaaagctc aatcacaata gatctggttt ttgttttcta aaataagtaa
    26221 ctgtttcttc gttgttcgct tttctgatag ctttggcaac tcttctatac taaaaaatcc
    26281 catgttggaa gtttcattat tctcaataaa cttggcctct tcaccgtgga tcgcacaagc
    26341 aaaaacaagt ttataatact gaaaaagctg tggaatatct ttttgtttat tcgtatcaaa
    26401 aactgctcgc aactgagtag tttctactgt taatcctgtt tcttcataga cttctttacg
    26461 cacattttct tcaggagaca atcctatttc agcaaatcca ccaggaagtg accattcttt
    26521 cgtccgttta tcttcgatta gtaggacctt gccatctttt ttgatatagg cacgaacatc
    26581 tacttttggt gttggatgtc cttctccaat cgttgtgagc gcttttattt cttcgaaagt
    26641 ttcttttcct atttcactga tcagttccaa tgaaatcgtc cggatctcct caaaacgctc
    26701 tttatcaaat acatcttttc cataatatag accagcttga gaaagtgcga gtagtctttt
    26761 gtattgagcc aacttcttgt tcattctctt cccttctttt ttagcaaatg tatgactatg
    26821 taatcaagta tagaagactg gtttaattac ggcaaataaa tataatgaaa tctgcaataa
    26881 ataaaagaat gttaaaaaac tcgagaacaa aagacattcg acttctctgg ggctgtgaat
    26941 aaatggcgtt caaaagcttg catctgcggt aataagcccc accaaacgaa aaatatgacg
    27001 agctattctt gtctggctgt tcttattaat tgaggcagga cagctttttc acaacctccc
    27061 cactaaacgg tgttctccta ccaactcctc ggtcacaagt cacaatattc atcaaacatg
    27121 agaagctgtt tttttgaaat tgctccttgt gactcggagc tgaacgacag gctcacgacc
    27181 tctcggtaaa cagcgttcaa aagccaattt ttcgatatta agccatttct ttgttactta
    27241 ttgtcattga tttctttttc taatttatcc aatagtggag tgtaatccac ttgagtatag
    27301 tctgttacat caaattcata tagtgttcct aactggaatt tgtcatattt tcggtcggcg
    27361 atgatctttt tgaatgcttc tttcgtaata tgattggtcc ctttcgttcg atcttccaat
    27421 acgtcaccat agtgataatg atccatgata tagctcaaat gacgatctga ttcacgatca
    27481 cgtttgtacc gacgttgcca taacacttca aagtctgctg ttaggcgaat cgtgatcacc
    27541 tgatatccgt atctatctgc taattcttgg agtcgtgctt tttgcttatc actaaaagga
    27601 tactcggaca caatcacttt ctttcctgct tccatataca aatcaagtgc tttgtaatag
    27661 aaaggataaa ctttcgtcct ttcttgttcc gcccgttctt ccaaattgtt aaacccgatg
    27721 gattctgcat agtattcctt gatttcatcg agcgtgatgg catacatatc tggaaatcgt
    27781 tcccgtagtt tattcataag atatgttttt cctgtaccag gactaccagc cagaagaatg
    27841 aaatactttt tcatctcatt tatttcctaa gttaattgga ctttccaagt tcataaacag
    27901 tccaatctta tgcttgacaa tttcttttac tgcttcattt gctggaatca cattatggcg
    27961 aagagacttg ttgatttctg tttcactaaa ggtttcttta gctactttcg tccagttgac
    28021 taaaagctcc gttccaacat tgaatttgcg gatcccgtta ttgatcaatt caggataatc
    28081 ttcttctttc acacctgttc ctccatggat aactaggggg acatcaacta ctgcatcgat
    28141 ttctttcagc aagggaatgt ttacttctgt ttttgatttg aattgtccat gattcgtacc
    28201 gatagcaata gctaatgcat ccacgcctgt agcttctaca aatttcactg cgtcttccgg
    28261 tcttgtatat accttgtcat tttcagcgac atggattcct tcttctgtac cgccgattgt
    28321 tccaagttct ccttctacgg aaacaccgtg cgcatgggca tattctacta ctgcttttgt
    28381 cttcaggata ttttctttga aaggcagatg ggacccatca tacatgacag aagtatatcc
    28441 gctgtcgatt gcttctttga tatcatcaaa gtctcgggcg tggtctaagt gcaagactgc
    28501 atcaaccatt tcttcttctg caatagctgt cattacattg actaacacat gcatgccgat
    28561 atatttagcg gtatcgacac ttgtttgaat aatgatcggc gaacccattt cttttgctgc
    28621 gcctagcata gcaggaagca tttctaggtt atgcgtatta aaagctccaa ctgtaaaatt
    28681 caactcttct gctgtttttg ttacttcttt caatgttgta tacatctttt attgccacct
    28741 tatcttttta tttgtttcct ttgatcgtta cttgttttgc aatttgtcgc atctcatcca
    28801 ttttgcccat gtagtaatcg atcccttgat catcaccagc acgagcagca tctgcacgct
    28861 tctggttata gaggctcatc agttttttat acccatctcc gatcgtctgt ttattttcca
    28921 aactcttttc taaatattgg atggcttgat cgttcacata caattcagaa aatttaagtc
    28981 caacttgttc ataaagtttc gctaatttat gcttatcttg ggtagtggaa atctcttgtt
    29041 tcaattgtgt aatctctgat tgaatctttt cggtcgcttc tggcgatagg gctttttctt
    29101 cttttgccag aacttttttc ggttcttttt cttttttctt aaaaaatcca aacatcattt
    29161 tccctacttt caattaaaat gctttcaaaa ctgggcttaa gatccaggca tataagagtg
    29221 ctgcggctac tgtatctaca tcggttgctg tagcattgtt gaatcccatc gaattaaaga
    29281 tcgttactag caaagcgggc aataatgtaa taaagaaacc atgagcaata ccgccaatga
    29341 tcgctcctcg gcgtccgcca actgcgttgc caaatatccc tgctgtccct ccggcaaaga
    29401 aattggttag cataccaggt aaaatcatcg ctaagccgaa catgggcaaa gtaaacatgg
    29461 caataatcgt accaatcgtt gtcgtaatga aacctaaaat gacagcattt gggctataag
    29521 gaaagaaaac tggacaatct aaggcaggga tcgcatcagg aactaatttc atagctatcc
    29581 ctcgaaatgc cggcacgatt tcacctaaca gtagacgtac accagctagc aatacataca
    29641 cacccactac gaattggatg gcttgtaaaa aggcaaacat gatatagttt tgatctcctg
    29701 ataattcaga agcgaatggt tcgcctgcaa ataaaacagt gatgatatat agcgggacca
    29761 tcacgaccat gacagacaaa taagtatctt gcagaaattc gaaatgtgtc ggcaacttga
    29821 tatcttcgat cgacttttta ttttcgcctt tctcaccaaa aagcttagca acaccggctt
    29881 caaataagta cccgatcgta caaaaatgac ctaaagcaat atcatttgat ccagtgattt
    29941 tacgaatgat cggttgcgct actgccggca tagcaatggc gaaacaagct ccgataaaac
    30001 caccgaccaa gatcaatacg atccctcgca gaccggcaaa atagccaaag actgtcgtca
    30061 tcgttgccat ccataaaatt gcttgaccag ttaaaaaaat gtacttccat tttgtgaaac
    30121 gagccaaaat aatattgaat acaaaaatgg ctaagaacgt cagtgcaata tctctaccta
    30181 aacccaaatc attcattgct tgaccattga tagattcaat agaagggatg atcccttgca
    30241 tatgaaatcc ttcagtaaag attttcccga aataagttaa acttccaacg atgatgctcg
    30301 aaccggcact caacacttga aatcctagga gtgtctttaa cgtgccagag atgacttgtc
    30361 ctgctgattt cttctgcagt agtagaccca acatagcaat caatgcaatg gtgatcgatg
    30421 cctgagtcaa aatattttcg atgataaaat tgataacgcc cat
//
LOCUS       DACEQH010000033        30169 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.033, whole genome shotgun sequence.
ACCESSION   DACEQH010000033 DACEQH010000000
VERSION     DACEQH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.033"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            25..402
                     /locus_tag="IW833_001549"
     CDS             25..402
                     /locus_tag="IW833_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307675.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            1008..1622
                     /locus_tag="IW833_001550"
     CDS             1008..1622
                     /locus_tag="IW833_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1307676.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            2395..4134
                     /gene="yfcC"
                     /locus_tag="IW833_001551"
     CDS             2395..4134
                     /gene="yfcC"
                     /locus_tag="IW833_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ1307677.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            4291..5475
                     /gene="iadA"
                     /locus_tag="IW833_001552"
     CDS             4291..5475
                     /gene="iadA"
                     /locus_tag="IW833_001552"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ1307678.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            5586..5789
                     /locus_tag="IW833_001553"
     CDS             5586..5789
                     /locus_tag="IW833_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1307679.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(5890..6552)
                     /locus_tag="IW833_001554"
     CDS             complement(5890..6552)
                     /locus_tag="IW833_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1307680.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(6565..8067)
                     /locus_tag="IW833_001555"
     CDS             complement(6565..8067)
                     /locus_tag="IW833_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1307681.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8615..10294
                     /locus_tag="IW833_001556"
     CDS             8615..10294
                     /locus_tag="IW833_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1307682.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            10421..10900
                     /locus_tag="IW833_001557"
     CDS             10421..10900
                     /locus_tag="IW833_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ1307683.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            11119..11799
                     /locus_tag="IW833_001558"
     CDS             11119..11799
                     /locus_tag="IW833_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ1307684.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            11807..13138
                     /locus_tag="IW833_001559"
     CDS             11807..13138
                     /locus_tag="IW833_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1307685.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            13153..14115
                     /locus_tag="IW833_001560"
     CDS             13153..14115
                     /locus_tag="IW833_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ1307686.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWEIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            14226..14564
                     /locus_tag="IW833_001561"
     CDS             14226..14564
                     /locus_tag="IW833_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ1307687.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            14643..15350
                     /locus_tag="IW833_001562"
     CDS             14643..15350
                     /locus_tag="IW833_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ1307688.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15453..16934)
                     /locus_tag="IW833_001563"
     CDS             complement(15453..16934)
                     /locus_tag="IW833_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1307689.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            17243..17779
                     /locus_tag="IW833_001564"
     CDS             17243..17779
                     /locus_tag="IW833_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1307690.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            17857..19227
                     /locus_tag="IW833_001565"
     CDS             17857..19227
                     /locus_tag="IW833_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ1307691.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            19294..19629
                     /locus_tag="IW833_001566"
     CDS             19294..19629
                     /locus_tag="IW833_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307692.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(19710..20567)
                     /locus_tag="IW833_001567"
     CDS             complement(19710..20567)
                     /locus_tag="IW833_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ1307693.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            20674..21189
                     /gene="tadA"
                     /locus_tag="IW833_001568"
     CDS             20674..21189
                     /gene="tadA"
                     /locus_tag="IW833_001568"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ1307694.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            21212..21296
                     /gene="ffs"
                     /locus_tag="IW833_001569"
     ncRNA           21212..21296
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IW833_001569"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            21506..22891
                     /locus_tag="IW833_001570"
     CDS             21506..22891
                     /locus_tag="IW833_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ1307695.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTDIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            22928..23605
                     /locus_tag="IW833_001571"
     CDS             22928..23605
                     /locus_tag="IW833_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ1307696.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            23613..24377
                     /locus_tag="IW833_001572"
     CDS             23613..24377
                     /locus_tag="IW833_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ1307697.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            24379..25038
                     /locus_tag="IW833_001573"
     CDS             24379..25038
                     /locus_tag="IW833_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ1307698.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(25068..25577)
                     /locus_tag="IW833_001574"
     CDS             complement(25068..25577)
                     /locus_tag="IW833_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ1307699.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(25689..26687)
                     /locus_tag="IW833_001575"
     CDS             complement(25689..26687)
                     /locus_tag="IW833_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1307700.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            26888..27472
                     /locus_tag="IW833_001576"
     CDS             26888..27472
                     /locus_tag="IW833_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1307701.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(27549..28724)
                     /locus_tag="IW833_001577"
     CDS             complement(27549..28724)
                     /locus_tag="IW833_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1307702.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            28806..29186
                     /locus_tag="IW833_001578"
     CDS             28806..29186
                     /locus_tag="IW833_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ1307703.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            29260..29622
                     /locus_tag="IW833_001579"
     CDS             29260..29622
                     /locus_tag="IW833_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307704.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         9843 a         5043 c         6240 g         9043 t
ORIGIN      
        1 tataaaaaaa ggtgtgtgtt cactatggga aaattagtcg ctgttgcaca gtcgctgctt
       61 gatatactca agtggctgat ggaaggaagc aattggatat ttgtgttgcc actattgctt
      121 attagtctat ttatcatgtt tcgcatggtc agaaaaaaag aaacgtttct gatttttatc
      181 tggtatgcag catccgttgg ggttgctacc ttgatggttg aattggcgtt aggtagttgg
      241 gaagcttgcg tatttgcttt attttttgct gtactgtatc taatcattta tatcccatat
      301 atgaaaagaa agtctcggat aggcagaaaa ggtagacata caactacttt ttctagtaca
      361 gattctctcg aacaaagtag aagaacgaga agaagacggt aatatttagt aggtatacgc
      421 aaaaaaagat aaaataatgg ataaactatt gagatgagag atttatttaa ttggtggaaa
      481 aaagcttttt tcacttatat aaaagaatct tttttaaagg aatgcttgtt gtatcgggat
      541 aagccattcc tttttttatg ttatttatat atatataaat cataccaatg tttttttgta
      601 ttttcttgct tgcacttgga taagagatta cttacaataa attcagatag aaacagctga
      661 ataaaaaaag gctccctgac ccttagttgg cgctaagaat cagggaatga ccagtcacac
      721 acatacaccc tgaccgatca ttttgccata gtcaatgcag tgcatcgacc taatttattg
      781 tacctaatct agggtgaaaa ttctacccct atgtagatat tttccatggt ctgcacgttg
      841 ttttatttgc tatgtcaagg tattgggcgt attcgttcaa tgcctttttt gtgtttgaaa
      901 cagcaaagac cacatgtgtt actgtctaac tactgttttt ttatgtactt ccttagatta
      961 ggcagctgtt tctattcatg tcaagtatag ctaggcggga gagataaatg agtttatgtt
     1021 attttgacta ttatcaccaa ttggaagaga aattaaggat agaagagccg ctgatgcttc
     1081 aagctggcac ttggatcgcg aaacaaatta atcaaggtgg tagattgcag atatatgcta
     1141 gccgcacact aagtggtgta gcatttgaat tttgggatca aaccccggat cttgtaccaa
     1201 gtatattgat tgaaaatcca gccggaggtg tctatgaaac gctagaaggt gcaggacagg
     1261 caattattga tcagctttct gtcaaatcag aagacatttt tcttcttctt tcgaatgaag
     1321 gaagaaaccc ggcaatcatc gaactagctc agtggatcaa agaaaatggt catccattaa
     1381 tcattgttac tggatttgat ttgtcacgaa gtatcaaatc gcaacatagc agtggtttgc
     1441 gattatttga atttgctgat ttggttttgg acaaccacgc caatttacat gatgcagtct
     1501 tgactttgcc aaatctagat ccagcgatct gcggaacagc ctctcttgca acgatctttt
     1561 tgctgcagca agttttgtat tttacagcaa aacagctagt ccaggacaca agtggaaaat
     1621 agttatccac atgttgacaa agttccttaa aaaaataatg ttccacataa aaatggagcg
     1681 tttgaaaaaa agaaaacgat tattaatgag gattaacaac gactactttt tttgttttag
     1741 ttattttaag atggatcatc tttgatttta agcttgtttt gtttaaagtt tgttattttt
     1801 ataagttgtt tcacagttat gagaaatgag ttataagaat atgtagtgtc agaaaaataa
     1861 aaagtagtgg aaaaagagac aagaaagaat ccatatggat tctttcttgt ctcttaatac
     1921 acgttgatga aagagtcttc cccaaaactc tttacaaagt aattatagta catttgttat
     1981 cattattgca atgaagttta cctgatataa caaaattgtc caacaggata cctttttcta
     2041 atagaaataa aatacacaaa aattgaagga tagataatgg atatttataa gtgtttatta
     2101 ttttttttgc aatgaaattc aatttttcaa tgattgtaaa atgaaaaaaa caggacgaga
     2161 ttttctctaa tctaaaaata aaaaaagaat tttaaggaaa aacagagaaa taaaatactt
     2221 aatataaatg gaaagagaaa aaaataatca tacataggag aaaaaaaaca aaatagaaaa
     2281 aagtgatttt ctaataaatt attcatgtgg aaatgtcatt tttactgtat aattcatttg
     2341 attatccatt ttttattcat caaaattact gaaaaaagaa aggaagaaat aaatttgaag
     2401 aaaacaaatt cttcaggaaa accgaaacgc gacttcacta aattatcgac accccatacg
     2461 tatgtcatta tttttggtgt agtcatcttt gcatggatac tgacttttgt cgttccagca
     2521 ggaaagttca gcacgcaaga tattgaatac aaagatgcaa atggcgaaac aagtactcgt
     2581 actgttttac gtcaagattc cttccgttat gcttacgagc tggataagtc gtatgtgttt
     2641 gatcaattag aagaactgca agatcatcca gcagaacgtg aaaaactgga tgttccggaa
     2701 aaaggattgg aaaaagtcat tgctgacgga gaaaaaaatc ttacgcagga aaaactagat
     2761 gaaatctcct tgacagatga tgtgctttac gatcagtacg gcgaaaatat ctatgatact
     2821 tctaaaaaat tgcataaaac agccaaaata tggggaacag atgattttgg cggctttggt
     2881 ttcttaaatt ttgtttttga aggactagta tctggagata agtatggatc tgctgtaggg
     2941 atcgctgcat tgatccttgt tgttggaggt gcttttggta ttattatgcg taccggagcg
     3001 atcgacgcag gtatctatgc gtttatcagt aaaactaagg gactggagcg tttagcttta
     3061 ccgctattat tctttgcttt ttcattcggt ggcgctacat tcgggatggc agaagaggta
     3121 attccattct caatggtgat ggttccattt gtcattgcgt taggatatga ctcgatcgta
     3181 gcagtaaccg tgacttatgt cgcatctcaa gtcggaaatg cgacctcttg gatgagcccg
     3241 tttagcgtag cggtggctca agggattgct ggaatccccg tgttgtctgg tgcgacattc
     3301 cgattgatta tgtgggtagt tgttactgca ttagccgctg gttatatgat gatatatgca
     3361 gaaaaaatcc gtaaaaaacc agaaagttcg ttgacttaca aatcagatga ttatttccgt
     3421 tctcatatca aaaagacatc tgatgaaaat aaaaaattca tgcttgggca taaattgatc
     3481 ttattagaga tgttagttgt ccttgtctgg atcgtttggg gcgtgactca aaaaggctat
     3541 tacattccag aaatcgcttc tcaattcttt gtgatgggat tagttgctgg aatcattgct
     3601 gttcttttca aattagatgg aatgaaagtc aatgacatcg cttcttcttt ccaatctggt
     3661 gcagctgatt tagctggtac agcaatcgta gtaggtatgg ccaaagggat tcttcttgtc
     3721 ttgggaggat ctgatgcatc tgttccttca gctttgaata cgattttgca tgggatcggg
     3781 acggcgttga ccggtgttcc agctgtcatt ggtgcttggg cgatgtacat cttccaaagt
     3841 ttgttcaact tggttgttac atcaaattca ggacaagcag ctttgactat gccaatcatg
     3901 gctccactgg cagatttagt aggtgtatcc agacaagtag cagttcttgc ttatcagctt
     3961 ggttcaggtt ttatggacgc ttttacaccc gtatcagcca gcttgattgg tgtgctaggt
     4021 gttgcacgga tcgaatgggc aaaatgggca agattccaaa tcaagatgca aggattcctg
     4081 tttgttttag gaactatttt catcatgatt gccattgcaa taggtctgca gtagtactgg
     4141 agacctgtaa ataaaaaagt aatccaaaca gtagataagg actccaagaa ccgtcatttt
     4201 acggtaaaat caaaggagta aagatcctat cgtttggatt attttttaag atcctatcat
     4261 cagttatact taaagaaaga ggaaaaatgt atgaaaatca ttcgacaaat tgaagtgttt
     4321 gcaccagatt acttaggaaa aatggatgta ctgattgccg gtgggaagat tttggctgta
     4381 gaagaacagc tagaaggcgg ctacgaagga gtggaagtag aagaactttc aggtgaggga
     4441 aaagtactaa ccccgggttt cattgactgt catttccata ttttaggtgg aggaggagaa
     4501 ggcggttatc aaaatcgaac gccagaagtc acattgagcc aattgactac agctggtgta
     4561 acgacagttg tcggttgttt gggaacagac ggagaaggta gagatatgac tgcgttgatc
     4621 agcaaagcga aaggtctgga agcagaagga atctccactt atgtatacga aggttcgtac
     4681 cgattgccgg taaaaacagt aacagattct atcatcaaag actttttgac aattgataaa
     4741 attatcggaa tcggagaaat cgcagtatcc gatcaccgtt cttctcagcc aagttttgaa
     4801 gagttcgctc gtgcagtagc agatgctcgt gtaggaggca tgttatccgg aaaagctggg
     4861 atcatcaatg tccacttagg cggaggaaaa agaaagctag agctattgac acgaatcgta
     4921 gaagaaacag aaattccgat cacacaattt ttgcctactc atgcaaatcg cacaccagaa
     4981 ttatttgaag cttgcgtggc atttgccaaa cgtggtggca cgattgactt cactgctagc
     5041 gaagatcctg atttttggga gaagacagat ggtgaagttc gcttcagcaa agcactgaaa
     5101 cgattgatcg aggaagatgt ttctttagat aatttcacga tgacctcaga tggacaagga
     5161 agcttgcctt attttgatga aaacaatcat ttcctagggt taggagtagg aagtgccaag
     5221 gcattgctag tagggatcaa agaagctgtg caaaaagaaa gtatcccttt ggaaatcgcc
     5281 ttacgagcta taaccagtaa tccagcacgt atattgaaat tggataaaaa aggaaaaatc
     5341 gaaattggtg ctgatgcaga tctgtgtatc ttggataaag aaacgcttga tattgataca
     5401 gttatcgcta aaggagaaat aatggtccaa gaaaaagaag tcaaagtatg ggggactttc
     5461 gaaaaaagtt tttaatcaac agctctaact atctttttta gaaaacgagc gttctgtttg
     5521 tctgtatctg ttgttgatgt taccctgaag gtagatcaat aaacaaacta agaggaggaa
     5581 gcacaatggc agatttaaaa ggacgttcta aagatatgaa agataaagta gtcggtgaag
     5641 gaaaagaagc atacggcaaa gcaacagacg acaagagcaa agaaacagaa gggaaagcac
     5701 aatcactagg tgctgacatc aaagaaaaag ctcgaaattt aggcgacgaa gtcaaagaca
     5761 agttttctcg aaaagacgaa aaaaaataaa aaccacttat tactaattga atcaagcaaa
     5821 taaaaaatcc gaaccatatc tgcgagacag atatggttcg gattttttat ttgatcatta
     5881 tcctgttttt taaacttcga ttttttgaaa ttttttattt tttagttcaa agacttcatc
     5941 acagcttttt gctacttcaa tctcatgagt cacgatgata atacagcgat tttgttcatg
     6001 ggcgatttct tggaaaagtt tgacgatatc ttgtgaagtt gtttcatcta gatttccagt
     6061 aggctcatcc gctacgatta gttgatgttc acagcagata gcacgaacga tagcgactcg
     6121 ctgctgctga cctcccgaaa gtttggtcac ctgttttttt gctaatattt catcaattcc
     6181 tacacgagcc agtcgatcta aagcaacttg tcgataattc tcttgcttgg tcttagtgat
     6241 ttccattgct gctatgacgt tgtctagagc cgataaataa ggaagcaagt tgtaagcttg
     6301 gaaaataatc gagacatcat ttttacgata acttcttaac ccgattttgt tcagtgaacg
     6361 atcttgatag tatacctttc cttcttttgg tgcatctagt ccagaaataa gcgaaagaaa
     6421 agtcgttttt ccagaaccac ttgctcctaa aatagcgtac atttttccag cttcgaaatt
     6481 cagttctaca tctttataaa gcgcatcttg cggatcttga taccaaaatc ccaattgttc
     6541 tgtccgtaac atcaaattcc tcctttagtt taataggatt tttcttgggt ttaatcttaa
     6601 tatccctata gaagctaaaa agacagaaaa cagactgatt ccaaatgcta ctccggctaa
     6661 tagtcccaat tgtgcaggtt tgacattgat ttccaattcg ctgacttgat cagaaacagc
     6721 aaatggatta ttcgtggttc cttgttgacc atcgcgttga ccgccttgtt cttgtctgtt
     6781 acctggcatt tgtccaccgc caggagcttg ctggttttgt gtcgtggttt ctgtctgttg
     6841 gtcaagtagc tgctgtccca cggcattcgc gacgacgttt ccgctaagcg aagcaactcc
     6901 taatgcaaga atcatacaaa tagccacttc ggtaaacaat tggagaatga ttttacttct
     6961 ggattctcct aaagatagca agacgcctaa ctcatgccga cgctcccgaa tggtaatcat
     7021 aatgatcaga gtaagtatga taataccggc tacagccact agtaaaacaa catttttaga
     7081 aaaacttgcc acgttgttta aaggttccaa cattgactga tacatggaat catttgattg
     7141 taagctaaat gtatctgtgt cgatcagctt ctcagcttct ttgagaaact catccatttt
     7201 atctggatca gatagagtgt aaatagcaga atctatcgtg ttatcgtcag atgtcccatt
     7261 tagttcattt gcaaaagtgt aagaagtgta aagtgtattc gacggattca tgaagttgaa
     7321 tttcataccc atactgccgc ttgtttcgct ggattcataa attcctttga tcgtcatttc
     7381 ataagtggta tcttcatctt cagtactcgt gattgtgaaa gtatctccga ctgccaggtc
     7441 atttgtttct gctagagtag aatcaatgac tacattgttt gtgcctttgt cgctgtcatc
     7501 tattccttca ccatccgtga tttttgctgt tccatcagaa aaattactat tttgtgcagt
     7561 tgctgacaca ccactgattt gaaagtctgc ttgagtcatt tgcggattcc cgcccattcc
     7621 tccagcaggt tcaccgcctg catcagtagt ttctgaactg tcttcacttg aatcagaact
     7681 agtgatggca gttattccat cttttgccat agctgaggcg gaactttcaa aggaataact
     7741 ctttacatta tctaattcag ctattttttc cgcgtccgtt aaggaaactg gtgtaagaga
     7801 aaaactgcct ggatctggac gttcttcagt ggtagaaccg cttgaagaag tttgttcctg
     7861 ttttttaaat gaagcttctc gattggcact taaagtcact gttgctccaa cttctttttg
     7921 tgcttgattc gctgcttctt ctgcagcgct tcgaatcgtg aggccagcta gcacaaaaat
     7981 aagaatggct gtgaacacgg caaacaataa caatgaacgt ccccatttta cttttgtact
     8041 tttccatgca cgtttcataa aattcatatt ttgcatccct cctagtagat gcatttgata
     8101 atagacaacc tatcttaaag ctagcttaaa tcttatgctg tttgcacacc atcaacatga
     8161 ggttctgtat atataaaaag tcactatagt tattgcaaaa aatgtggtaa aatgaaaaaa
     8221 gtatgtttca aaccgacgaa gaagaaatga aagaggaata gcaaaagaaa tcctagatgc
     8281 tacagaggga tttttacgtg catagttgtt ctaacaattg ctgatactag cgatttagtt
     8341 tatttgttgt ataataatgg ggaacagctg aatacttata taagtattct ggctatagcg
     8401 tttcgcaaaa gatccatttt gttaatgaag tatatccaaa ataaaatgcc catccaactg
     8461 tcagaacgcg gatgtgctgg taattttcct gctgattctc cataagttga gaaatagtga
     8521 ttttgcatat gcttcattgg aatggctcat atatttagat aaagaagaaa atcgacatct
     8581 ttaaacttgg atgtcataag aagaggtgaa gtttgtgagt accattaaaa ttatcccatt
     8641 aggcggcgtt cgtgaaaatg gaaaaaacat gtatgtcgca gaagtgggcg aggatatttt
     8701 tattttagat tgtgggctaa aatatcctga aaatgagttg ttggggatcg acgtcgtaat
     8761 ccctgatttt acgtatttag aagaaaacat tgatcgtgta gctggtgtct ttttaacaca
     8821 tggacatgca gatgcgatcg gggcacttcc ttacttgttg tcaaaaattt ctgtaccagt
     8881 attcgggaca gagttgacga tcgagttagc aaaattgaat gtcagtcgta atgaagcagc
     8941 caaaggattt aaggatttcc atgtggtaga tgagcataca gaaatagact ttggtgaaac
     9001 agtagtgagc tttttccgta ccactcatac gataccagat tcagtgggga tcaatctgaa
     9061 aacaaaagaa ggtagtatcg tttatacggg agattttaaa tttgaccaaa cggctatccc
     9121 aatgtatcaa acagattatg cacgattggc ggaaatcggc aaagaaggag tcttagcctt
     9181 actaagtgaa tcggcaaatg cagaaaatcc tcagcctgta gcttctgaag cccaaatagc
     9241 agatgaagta tttgatacga tcaaatattg ggaagggcgt atcatcgtcg cttgtgtagc
     9301 aagtaatttg caacgagtgc aacaagtatt ggatgcagca gaaaaagcac aacgaaaagt
     9361 tgtcttgact ggtcaagatt tcgaacgaat tattcgcaca gcgatgcgtt tagaaaaact
     9421 tcaattacca agtgaagatc tgcttgttac gttgaaagac atgaaaaact atgatccaga
     9481 agaactgatc attttagaaa ctggccgaat gggtgaaccc atcaagtctc tgcaaaaaat
     9541 ggcaaacggt acacaccgta atgtcaagat tgaagaagga gatttggttt atatcactac
     9601 tacgccaagc atcgcgatgg aaaccatcgt ggcgaaaacg gaagatatca tttatcgagc
     9661 tggtggaaca gtgaagttga tctctgagaa tatgcgcgtt tctggccatg ccaatcctaa
     9721 tgacttgcaa ttaatgatca atttgatgaa accaacatat tttgtgccag ttcaaggaga
     9781 gtatcgtgaa ttagctgctc atgcagattt ggctcatgca gtagggatgc catacaaaaa
     9841 tatctatatt actggacgcg gagacgtgct ggaatataag aacaagcgta tgagtgttgc
     9901 aggaactaca agtgcagaaa atgtaatgat tgatggatta ggcataggtg atatcggaaa
     9961 cattgtttta cgtgatcgaa aaatcttgtc agaagacggg attttcgtag cggttgtcac
    10021 tatcagtcgg cgagaaaaga aaatcatttc aaaacctcaa attacttcac ggggattcgt
    10081 ttacgtaaaa gctagccgtg atctgatgaa agaaagcagc aatatgattg aaacaatcgt
    10141 tgagaaacat ttagaaagca atgattttga gtggagcaaa ttaaagcaag acatccgaga
    10201 tcaattaggc cgttacttat ttgaacaaac aaaacgccgc ccagtgatct tgccagtcat
    10261 tatggaagct actcaacgtc gaaataaaaa ataaacaaaa agagtaagat gaacctctct
    10321 atagagtgct cgtcttgctc tttttttgcg aaaaacaaat aatttttgaa tacagcatcc
    10381 ctcgctttat aataaacgat agaataaagg gagtgaatca atgatgagtc aaacaaaagt
    10441 cgaacgttat ttagaagaac aaaagatcag ctatcaacca cttgatttta gtcatgtggt
    10501 agaagaatct ttcacagaag aactacaaaa aagagggatt gatccagctt tgatatgtaa
    10561 aactctcgta gtgaaaggaa acaaaactgg ggtgatcatc gcattgattc ctttgaacga
    10621 tcacttagat tacaaaaaaa cgagacaagt gacgaaagat cgtaaagttg gttttcctgg
    10681 aatggatttt gtattaatgc atacaggata tccccacgga gcaaacaccc ctattggaat
    10741 aaaactcgca catcctgatt attgtttttt agcagacagt tctttaaaag aaaaagaaga
    10801 agtaattgtg tcaagcggtg aaatcgggag aagccttcgg ataaaagtag ccgatttaga
    10861 aaagatcgtc catcctattt acgctgattt aattagttaa gggacgagaa gagaagctaa
    10921 gcagagcaga gacctcaaac ttgagattat atctgcttgg tacttggtgc atcctgtatt
    10981 atctgttaca attgctttgt acatactgta ctatcaatcc aatgaaaata tatttagcag
    11041 ctatattttt tcattgatgt gcaagtgatg aaatcgtttt taataaaaaa gatacagaga
    11101 aaacgcgagg tgtaaaatat gaaaattacg tatcatggac attcctgttt aacaattgaa
    11161 atggaaaatg gccaacgcgt gatgattgac ccgtttatta caggaaatcc attgacagac
    11221 ttaactgcag aagaagtaaa gacagattgg ttgctgatta ctcatggtca tagtgaccac
    11281 ataggagata tgattcctat tgcaaaagaa aatcaagcca caattatcag tattgttgag
    11341 attgctgatt atgcgaacag cagaggagta gattcattcg gtatgaatat cggtggaaaa
    11401 catgcgtttc cattcggcgt ggtaaaattc gtacatgcac agcatagttc aagctatgaa
    11461 gtagacggta tcgttcagta tatgggcgag cctagcggaa ttattattca agcagaagga
    11521 aaaacgattt atcatgcagg ggatactgct tatttcagtg atctaggatt gcttgcagaa
    11581 gaattcgata ttgatgtcgc tttcttgccg attggcgata actatacgat ggggccagaa
    11641 gatgcagcaa aggctgcgca aacaattcgt gccaagaaag tagttccaat ccattacaat
    11701 actttccctg tcattgaaca agatcccgaa tcttttgtag agttactgga agatagtgaa
    11761 ggaaaaataa tgagcgtctc ggaaacgctg gaagtgtagg cagaaaatgg cgacaaaaca
    11821 tgatttgatt ttagaatata ttgaaagttt acctgttggc aatcgaattt ctgtaagaag
    11881 catcgcaaaa gaattgaatg tcagtgaagg aacagcgtac cgtgcaatca aagatgcaga
    11941 aaatgtcgga ctagtttcaa caattcaacg agtagggacc atccgtatcg aacgcaaatt
    12001 aaaaaaacat attgaacgtt tgacatttgg cgaggtcgtc aggatcattg aaggggacgt
    12061 tttaggcggg gcggcaggat tagacaaggt cttgaataaa ttcgtcatcg gcgcgatgac
    12121 taaacatgcc atggaacgct atattacgcc aggatcacta atgatcgtcg gcaaccgggc
    12181 ggaagtccaa aaattagcat tggaagatgg cgccgcagta ttgatcacag gaggattcaa
    12241 tacgactgct gagattgcaa agctagctga tgatcttgaa atgccgatct tacgcacctc
    12301 ttatgataca tttacagtcg caaccatgat caaccgagcg ttaagcgacc aattgatcaa
    12361 aaaagatatc atgcttgtca gtgatatcta tatgccagct gaaaagactc attatttgca
    12421 gcagacagat acgatttctg attatcagcg tttgtcagaa gaaacgcagc attcgcgttt
    12481 tccagtcgtc aaccgccatc atcgtttgat ggggattgtt acggcaaaag atgttttagg
    12541 gaaatcgcca aaccagatca tcgatcgggt gatgacgaaa gaaccaatca gtgtaaagaa
    12601 aacaatgagt attgcctctg ttagccatca aatgatctgg gatgggctag aagtcatgcc
    12661 ggtcgtttcc gatgacttga cattggaagg cttgattacc cggcaagatg taatgaaagc
    12721 gatgcagctt gtccagcgac aaccgcagat tgcagatacg atttcggatc agatatctgg
    12781 aaatatccat acgattgata ctgaccgtga aggaaatcgt ttggaaaacc ctaagttcaa
    12841 attcgtagta gcaccacaaa tggtcaatgg cgttggaacc atttcttttg gcgtacttag
    12901 cgaaatcatt tctgatgtcg cacaaaaaac aatggtgatg aaccaaaaac gaaatatctt
    12961 gattgaacaa gtgaacctac attatttgcg gctgatccaa ttggaaagcg aattagacat
    13021 tcgtccacgt gtattggaaa tcggcagacg ttccgctaaa ctggatatcg aagtgtttat
    13081 tgaaaacacc atcgtttcca aagcaatcgt tgtttgtcaa gtaatggaac gttcttagaa
    13141 agcaggagtg aaatgacagt acagcaagaa atagtagata caataaaaaa atacgataaa
    13201 atcattatcc acaggcattt gcgccctgat cctgatgcat taggatcaca agtaggatta
    13261 gccgaactat tacgggaaag tttcccagaa aaagaaatac gtcaagtagg tgaaacgatt
    13321 gaagggcttc gctttttagc agaaatgcaa gaagttgaag atgtcttcta ccaaggagcg
    13381 ctagtcatcg tgactgatac agctaattca cccagaatca gtgatcaaag atatcagttg
    13441 gcagaaaaac tgatcaaaat cgaccatcat cccaatgatg acccttatgg agatcttgtg
    13501 tgggtaaata caaaagcaag tagctgtagt gagatgattg cagaatttgc tttgatgttt
    13561 cctgaatctt taaagatgaa cgttaaggct gcacggttac tttatgcagg aatcgtcgga
    13621 gatacagggc gctttttgta tccctccacg acgtcccgta cattagagat tgcttcgata
    13681 ttgagaaatt atccatttga tgcaagtgct ttgaacagag agattgaaca aatgccgatg
    13741 aaagtcgcta aactttccgg ttatctttat caaaatatcc aggtggatga aaatggtgca
    13801 ggaagaataa tccttccgca agaaattctt gataaatatg gaatcgatga ttcagagaca
    13861 gcttcgatcg tttcacttcc tggagtgatt gacgaagtgc tggcttggga gatttttgtt
    13921 cagcagcctt cagggtatta ccgcgtacgt ttacgttcta aggggccgat catcaatgaa
    13981 ttggctaaac gccatcatgg aggaggccat ccattagcaa gtggtgcgaa tgcaaaagat
    14041 ttagcagaag tagaggaaat ctataaagag atccaagata tatgccaatc gtataagagc
    14101 agaaaaacag aatgatactt cgttcgaaaa cgtgcctttt gtagtaaaaa tcacaaatgt
    14161 atcttttcaa gatcttcttt ttccgttaca atagtgctca ctaaagattt taaaggagaa
    14221 agaagatgac attaccaaat tgcccagaat gtggctcaga gtacacatat gaagatcgag
    14281 gcttgttgat ttgcccagaa tgcgggaacg aatggaacaa agaagaacaa gaaactgtca
    14341 tagaagacgg attagtggta ttagatgcaa atggaaaccg tctgttagag ggcgatagcg
    14401 tgacagtgat taaagacttg aaagtaaaag gtgcgtcagg tgccattaaa caaggaacaa
    14461 aagtcaagaa catccgttta gtagaaggcg atcataatat cgattgcaag gtagaaggat
    14521 ttggaccgat gaaactgaaa tcggaatttg tgaaaaagaa ttaaagaata ttcaaggtct
    14581 tttcacattc ttttcctact actaatgata aaataaagta aacgaaaagg agtgaaacga
    14641 aaatgtatgg ttatggaatg tatggttttt tagatccgac atttcttttg gtcattcttg
    14701 gattagttat ttccggaatt gcgtcagcgt acgtcaatag tactttccga aaatacgatc
    14761 aagtgagaag caaaaacaat gtgactggta cacaagcagc acaatatata ttacaaagtc
    14821 aacagataaa taatgtcggt gttcaacaaa ttgccggaga tctcacagat aactacaatt
    14881 caggtaataa gatgttgagt ttgtctcaag caactgcaca gtctacttca gttgctgcga
    14941 taggtgtagc agctcatgaa tgcggacatg cggtacaaga tgcttcgggt tatgtcccat
    15001 tgaagattcg tgcagcactt gttccagcag ctaactttgg ttcgatgatt tcgttcccct
    15061 tgattatggt aggtgtattg cttagctgga actctacgtt aatcaatatc gggattttcg
    15121 ctttttcttt agcattactt ttccaattgg tgacattacc agtcgaattt aacgcgtctc
    15181 ggcgagcaat ccaaattttg agtgaaggcg gactactgac ggaagaagaa gtaccgatgg
    15241 caagacacgt attatttgca gcagcactga cttatgttgc agcagcgctt tcgacattcc
    15301 tgcagctgtt gcgcttgatc ttattgttcg gcggcaaccg aagagattaa aataaagaag
    15361 ttgtgaaatc agcgctttga tctgagctaa taccgagagt tagctgattg aataccattt
    15421 atttggaata agaggatgtg acaagaattt tgtcacatcc tctttgtttt gttctcataa
    15481 ccttttttca tatacagttc atgaaccgct tttattaact tgccaaataa cactgtattg
    15541 tgcgttgtat ccagatagaa agtctccgtt tcatctgaaa ttttctcatg ctgatctgta
    15601 atgatcaaat cagcatgttc tgaatatgcg gtaattttaa cagcatcttt gcgaaataac
    15661 tgctgcaaat ggctttgaag atgataattt cctgtaaact gtttggttat ctgcaaatgg
    15721 atgagtaact gctctttcgt ttctgtttgt agtaagtgtt ggatcaattg actaaggtat
    15781 tgactgattt ggtttctttc cccatttttt gaaaaaattg tatcgatttt ttctcttaat
    15841 ttcttttcct gtataaaaga actagaatga ttggacgtgg cttctggaaa gcaaagtgta
    15901 gaaaaactac tgctggattc acccaacagt tcgaaaaaaa caactgccaa cacaagaaaa
    15961 taaaaataaa ttggtatttt attttctaaa tggttgagag gaaactgttg agttaagtaa
    16021 gtcatcaatt gaccgacttg tcgacaaata aggtttttgc ttgtggaaaa taaaaaacca
    16081 atctcctgtt tttgtcgaaa agagatttga tttggaaaaa aacaatagaa aaaataatta
    16141 aagtaggctc tctctgtttc tgtctcttca caagggcgac tcactgaaaa agaagtaaag
    16201 tattctgaat aatcatgtgt atgctgcaat aagtccatca attcagtgat ttcagaagaa
    16261 gtttttggaa aatatgcctg ttgtttgttt cgtaatatga ataaagttaa cagcaagtag
    16321 atattttttt cttgttttga aattttttct atttgctcat ctgtactgtt catttttagt
    16381 gcatttaatt caagtgagga aaatggccaa tcgttacctc caaatgtatc tgaaagaaaa
    16441 aagtaaagat agagacggat agataactca tttccttcaa gagtacagca attttttaca
    16501 gataggatcc gtaaatggaa tttttttaag gaatgattga gctgttttgt cagtttttcg
    16561 agataggagg gcgatatata aagttgttca agtaaatatt tttttggtac agcagtttgt
    16621 ggataattga ttagtaacat caaatagtta aaacgattgg attcttttag gtagcataat
    16681 tttaacttag aaaaaacagc taacttttta gtttgatcgg tttctttggt ttggatctct
    16741 cctttatgat tagaataaat aaattcatca atcaataaaa attgcttgag tgatacattt
    16801 atttttcgga tcagttcttt gattgtccgt tttggaatat gtaagtcatt ggataattct
    16861 tgtaaagaac aattattttt ttgaatgacc atttgtagaa caagtaactc tttttttctt
    16921 gcttcaatta gcataaatac ctccttttta ttctgttttt gtatatagtt agacaaaaac
    16981 tgtgagtgga aagtcatttc agtctgtgtt attctcacag agtgtcgcct tcttttttat
    17041 ataacgttat ttaatttttt tgttaccttg tttattctat tgtttatttc ctagggtatt
    17101 ttgtcaagat attcttgttt ttaaggcgga aaaattgatt tttaatggat ataactaaat
    17161 atgttttgat aattatcaat aatattttta gaattattta tgttatttat ttatgggtag
    17221 atgaaaagag acggaggtaa gaatgatggg gagagtaaca aattatagta aagaatattt
    17281 gctgttcaaa agtatggtat acgtggaaga atatggaatg aagtcaatta ctgcaagaga
    17341 gttagcagag ttctgcggtt gttctacata cccgatttat acacatttca aatcaatcag
    17401 cgggttaaaa gagaagatat tagaagaaat cactgtttat tttgaaaact acttagctga
    17461 atgcagttcc gatgatgttt taagcattgt ctacttattg aaagattttt tttctatcca
    17521 tgaaagtatt cgtgaaatca ttgcaaaatc tgatttagac atggattcgc tatttaagcg
    17581 gccatttttc tcatatgtca aaggtcacac gaagatcaaa gaagaaaagt cgcttgaatt
    17641 ggtatggctt aatgcattgg gaagtttgcg ctcagatgat tcttctcaag attttcttga
    17701 aatgatgaac gctgtttggg ataaaaacga ggtgacggat gaatacttgg aaaatatctt
    17761 aaaggaacag tcaccttagg cagcaacatg aaaaagtaga tcagccacct ttatttttcg
    17821 taaaaaagat tgtaaaatgt ctaacgaaac attggaatga aggaggaagc gacaacgtta
    17881 ccacagcaat ttatcaaaaa atatcgtctt cttttaggcg aagaagcatc cgattttttt
    17941 tctgcgcttg aacaaggaag cgtgaaaaaa ggatttcggt ggaatccact aaaaccggca
    18001 ggattggaca tggtccaaac ttaccatagc gaagaattac aacccgctcc ctacagcaat
    18061 gagggatttc ttggcactgt caatggaaag tctttcctcc atcaagctgg gtatgaatat
    18121 agccaagaac caagtgcaat gatcgttgga acagctgctg cagcaaaacc cggtgaaaaa
    18181 gttttggatt tgtgtgcggc tcccggtggt aaatcaaccc aattagctgc tcaaatgaag
    18241 gggaaagggt tattagtgac gaatgagatc tttccgaaac gtgcgaagat tttatcagaa
    18301 aatatagaac gatggggagt ttcgaacgca atagtgacaa atcatgcacc cgcagaactt
    18361 gtccctcatt tttcgggctt ttttgatcgg atcgtggttg atgctccttg ttcaggagaa
    18421 ggaatgttcc gaaaagatcc aaatgcaata aaagagtgga cagaagaatc tccgctatac
    18481 tgtcaaaaac gtcagcaaga gattttgtct tcagcaataa aaatgctaaa aaataaagga
    18541 caactgattt attcgacttg tacgtttgca ccagaagaaa atgaagagat cattagctgg
    18601 cttgtagaaa attatccagt aacgattgag gagattcctt tgactcaatc cgttagttca
    18661 ggacgttctg aatggggaag cgtagctggt ttggaaaaaa caatacgtat atggcctcat
    18721 aaagatcagg gtgaaggaca ttttgttgca aagctgactt ttcatggaca aaatcagatg
    18781 cataaagaaa agaagacacg aaaaaaaagt aaagtgcaaa tgacaaaaga gcaggaaaaa
    18841 ctctggacgg agttttcaaa tgattttcat tatgaagcta ctggaagact tttagtgttc
    18901 aacgaccatc tttgggaagt tcctgagtta gccccttctc tcgatggttt aaaagtcgta
    18961 cgtacaggtt tgcatttagg agatttcaaa aagaatcgat ttgaacctag ctatgcatta
    19021 gcgcttgcta caaaaaagat agagaatata ccttgcttgc cgatcactca aaaggaatgg
    19081 caatcttaca cagcaggaga aacatttcaa cgtgatggga atcaaggatg ggttttgcta
    19141 gtattagata agatacctgt cggatttggg aaacaagtaa aaggaacagt aaagaacttt
    19201 ttccctaaag gattgagatt tcactgagtg gattagttcg tttatttttt tgtctatgct
    19261 atgatggaga tagataaaaa ggagctgata cttatgaaaa catcaacaaa agtaacaatc
    19321 ggattaagcg tagcagccgc tgccagtgta gcgacagcag tcgttgtatc gggaaaagta
    19381 atggaaaaga tccatcatat gactactcgg acgaaagtaa aaaaattcgt ctatgacaaa
    19441 tttgatggaa atgaaaaatt actagaaatc gtagatggtc tttcagatag tgacttggat
    19501 tctttgatgg gaatgttagc aaaaatcaaa tcaggaaaaa agaaaatctc tgtttatggt
    19561 gattcaatca aagattcaac cgaagatgta aaagaccggc taatgcattt tgttgaaaaa
    19621 atgatgtaag agaaactagc ttttgaacac tattcattcg aaataagggg ttgtgacaaa
    19681 attattgtca caaccccttt aatctatctt taattatgtt catgaagcaa taaaagatag
    19741 caaaacagta aatcgttggc tttttttaga ttgaaaccag actgttcttg aaatttttcg
    19801 attcgatatt ttagagtatt acgatgaaga aacagtgttt ttgcagcaga gctgacattg
    19861 cctaattcat tccataattc ttgaatgatc acttccattt cattttgttt gatcagttct
    19921 atgtaacttt tgatcaactt actatctttt acagtatctt ttgtatagta atgtaaagcc
    19981 acatcttgca tagaaaaagt acggctgtga gtataaagat tgttttgctc tgttaaaaaa
    20041 atttttcttt cttcagcaaa caaagggacg aggtcggtgc caggtgaatg gaaagaacca
    20101 acgaatacag acgttgtcgt atcaaaatca gcatctaaag atagaaaaat accgttgatt
    20161 tcagtagcat caagataatc acggtttatt tgttcgacta agagcgcatc tgtgtctgta
    20221 taaaaaaaga aatcagcaga atagcgaaat aattccaaaa tagtatcttg ccattctttt
    20281 tttaagaatt ctccttttga ttcgacgtga agctggatca aacgaaagct tttttcttga
    20341 acacaagaag catctttaaa aagatagtga taccatgggt gttttttttg atcgttgatt
    20401 acagggaata acgctttcaa aaggctggtt tcttgttgag ataatgaatc ttgtttgatc
    20461 cagataaaag catcatcaat tgcaaaagaa aggatatccg cttctgatgc tggagagttt
    20521 tttttctgtg cttgtgggta aagagcaagt aatttatcca ttttcatctt gttcacctct
    20581 tgtatttatt ttaccataga aaaaaaagtt agttacagcc tttttatcat atgctaaagt
    20641 aaaacaagaa aaaacaaagg cagggaaggg taattggtaa atacaaatct tgatgcagta
    20701 gaaaaagaaa aatggatgcg ccttgcattg gcagaagcaa agaaagcaga aatgcttcat
    20761 gaagttccga ttggagcagt ggttgtattg gatggaaaag tgattggaag aggatataac
    20821 cttagagaaa ccacgcaaga tgctactaca catgcagaaa tgcttgcaat caaggaagcc
    20881 tgtgaaaaag tgggaagctg gcgcttggaa gatgctgcac tttttgttac cttagagcct
    20941 tgtcctatgt gcagcggcgc gatgattttg tctcgtgtag cagaagttta tttcggcgcg
    21001 tatgatccaa aaggcggaac agccggtacc ttgatgaatc ttttggagga cgagcggttt
    21061 aaccaccaag catatgtaga aggtggcatt ttagaagacg aatgtgggat gattttgact
    21121 gactttttcc gaaagctccg ccagcgaaaa aagaaattaa aaaaagactg gtcaacgaat
    21181 gaaaaataag gtatactatt ctttgccgaa aggctaggac aatggtgggc ttgtgaagcg
    21241 tgtcaggtct ggaaggaagc agcactaagc aggttccgcc atgtgtctga ttataatgaa
    21301 acgacaacag actttcgggt ctgttttttt tatttttata gtactgtaaa aatgaatgtt
    21361 cctgattttt tagccagact gatttatcga atattgtact tatacaattt gcttcaaggc
    21421 aggaaaaact ctgaaataga gcttgtttca atggaagaaa aagctgccta tggtatgata
    21481 aagataaaga gaggcgagag ggtacatgga aaaaatagtg attttacata cgaatgatct
    21541 gcattctcat ttagaaaatt ggccaaggat cagaaggttt ttggatcaac gtaaacgaga
    21601 aaacgaaaag aaagaaaaaa cgacaacgat tgcagtagat ctaggtgatt ttgtggatcg
    21661 ttggcatcca ttgtcagaag caacgaatgg acaggaaaat atcttgctga tgaatgaagt
    21721 tggttatgat gcagcgacga taggaaacaa tgaaggcgta ggaaattcca aagaagagtt
    21781 gaatcattta tacgatcatg ctgagtttga tatcatcctg gataacttat tcgataaaaa
    21841 tacgctccag ccaccggcat ggacgaaacc atataaaatc attacaacga aacaccagac
    21901 gaaaatcgga ctaattgcat ttactgctcc atttccgctt acttataatc cgaatggctg
    21961 ggacatacgg aatccttatg atattttgcc agaattagtc gaagatttac gttcacaagt
    22021 ggatgtttta gttttgatga gtcatctcgg gattcaggat gatttccaaa ttgcaaaaga
    22081 aataccagca attgatgtta ttttaggatc gcacacccat catttattca gagaaggtca
    22141 cgtaatcaat gatgtccaaa tagcagccgc tggaaagtac gggcagtatg tcggtgaagt
    22201 ccatttaacc atcgatgaag agaaaaaaat ccttaagcat tcagccaaag ccatccctac
    22261 cgaaacaatg acagctttta cagaagatga acaagaaata aatagttatc tagaacgtgg
    22321 gcatgaatta ttaaaagaaa aagaagtagc agatttgcca ttttctctga gtctagatat
    22381 cttcaatgag cattcgttta tccaagtagc ctttgaggcg gtgaaagaac gaggaaagac
    22441 ggaagcagca atattaaaca gtggtctgtt tttaactgat attccaaaag ggcgggtcaa
    22501 tcaggatcag cttcacactg cgttgccgca tccgatgcac ttgttaaatg tcacattggg
    22561 tggaaatgat ctgatccgct tagttttaga aatagaaaaa aatcgtaact ttttacgtaa
    22621 ctatcctatg aagggaatgg gatttcgcgg taagattttt gggcagatgg tctataatgg
    22681 aatcacatat gattctatta accatcaagt cttttggaag aacaagccaa tcgatctgaa
    22741 taaaaattat acttttacta ccgtagatca tttgatgttt gttccatttt ttccaacgat
    22801 tgagattgct ggaaaaaatg aatttttgtt ccctgaattt atccgaagtg tggtaggaga
    22861 ttacttgaaa gctcactacc caatcaaata aaaacaaggt ataataaaaa agataggtgg
    22921 tgaacgcatg tctgcaaaac aaaatgaaca gcagaacgtt gagcataaaa cagaaattgc
    22981 taagaaaaaa tcatcagctg aacaggaaac aacagtcacc gaagaactga cagctgcttt
    23041 ggaagaaatc gttgaggagc ctaaagcaga aaaagtcaaa ggcgagctag tgacactgtt
    23101 agatgacagt aattttttga taggtgaaag acattatcgg cttgtgtccg attatcgtga
    23161 aggatttaat gcagaaaagc ttggagaacg ttacagtgat gtactagccc gatatgatta
    23221 tatcgtagga gattggggtt atgaacaact acgcctaaaa ggatttttcc aagcggataa
    23281 tcgacgggcg catccagatc agcgaatcga ttccttggaa gactatcttt acgaatattg
    23341 caattttggt tgtgcgtatt ttgtcatcga gagaatcggc ggtaaaaaag aaaagacaac
    23401 tcagcgccga aaaaagaaaa aaaaccataa tcgtgcgtat atcgatgaga aaaaaggacc
    23461 agtcgcatct aatcgcaaaa aaccggttat taaaaatcga aaaacagagt cagcaaaaac
    23521 ctctgaaaaa gtaaaaaaac aagaacaacc taaacaagta gaaaaaacaa aaacagcatt
    23581 tacgatcaga aaaagggaag agtagtggat gaatggacta taaaggatat ttaattgatt
    23641 tagacggaac gatttatcgt gggacacaac ccataccggc cggtaaacga tttgtggaag
    23701 aattgcaaaa aagaaaatta cctttcttgt ttgttaccaa taacacgact aagtcaccag
    23761 aaacagtagc taatcgtcta gcagatgagt ttgacattca tgtagctcca gagacagtct
    23821 acacagctac tttggcaaca atcgatttta tgaaagcaga tggaaaaggg aaaaaagtct
    23881 atgttatcgg agaagcagga ttgattgact tgattttagc tgccggattt acatgggaag
    23941 aagaggctcc tgactatgta gtggtaggct tagataatta tctgacctat gaaaaagtag
    24001 ttaaagcaac acttgctatt caaaaaggtg ctactttcat tggaacgaac ccagataaga
    24061 atatacctac tgaacgcggc cttcttcctg gagcagggtc agtcatctct tttgtagaga
    24121 cagcaaccca aacgccgccg atctacatcg gaaaaccaga agccatcatt atggataaag
    24181 ccgttgaagt attagggctg caaaaagaag aagtaatcat ggttggagat aactatgaaa
    24241 cagatatcca agctgggata cgcaataata tagatacttt gctcgtttta tcaggtttta
    24301 caaaagaaga agatgtacct gggttgccta tcccagctac ctatgtgaaa caatcactgg
    24361 atgaatggag cttctaatat gaaaaattcg agatggcagt ggatggaata tgctggattg
    24421 ttcagcctat ttctgacact gatcagtctg gcagttggtg tgacgatcaa ctttcgacca
    24481 ctatacgtat ttgatatcgg acatttgcaa attttggatt atacttcttt agatcaagag
    24541 acgttactga aaaattttga tcacctaatg aattatctca ataatccctt caaaaccatc
    24601 ctcagcttgc cagatttccc agtttctgct agcggagccc atcactttta tgaagtgaaa
    24661 attttgtttt tagtggatta tgctgtgttt ttcatcactt tgatcccgag tattttgttt
    24721 atcaaatatt tgcaaaagaa tgatcggctt tggcggttga ttcgtccgtt tcaaatcggc
    24781 atgttactgc cagttgtttt cggttttttc atgatgatag gatttgatcg cttttttatc
    24841 ctttttcatg aaacgttttt caacaacgat gactggttat tcgatcccgt tactgatcca
    24901 atcatcaatg tacttccaga agaatttttc atgcatagct ttatcttatt ttttgtatta
    24961 ttagaattat tttttgctgt ttttcttttc ttaggaaaaa atagcttgaa acaaacaaaa
    25021 aagaaagaac tcgtctaaat agacgttttc tttctttttt tgttgaatta ttgagtaagg
    25081 cgacttcttt tttctggttc tttgtgtgct aatcgactgg cagcagcagc ggcgatcgct
    25141 ccgacgatat catctaagaa ggtatggaca tgaacgccat cgtggctgtt tagttctttt
    25201 aaaataccag gtttcacctt gtcgatataa ccataattcg taaagccgat cgtaccgtaa
    25261 acattcacga ttgacaaggc taggatctca tcgataccgt aaaggccttc gtcatcttcg
    25321 ataatatgtt gcaacggact catcaatttt ttcttttctg ctaatatatc taattggatt
    25381 ccagtgataa tggcgttatg tacttcacgc tttgttagca cgctgttgac actttcagta
    25441 caatccgcca aagtcaaatc agaaatatag tctttttgta aaaacatgac aagttgtgcg
    25501 atatcttcta cagttacgcc acgttccttc aatagttctc ttgccttttc ttctaaagtc
    25561 tcagttttga cgaccatatt attgctcccc ctttattttt tctttctcta tttcatgtta
    25621 acggaacatg aaaaaaatgc aagaaactgc tttcgggttt aacttgaaat gcaaagagat
    25681 ataaaaaatc aactaggacg aagattttta tcgtataagc ttgtgctgtg accaggctga
    25741 gttcgttcct ttggattaat atagtgtaaa gcgttgttca ctgcagtagg tgcttcaccg
    25801 agcccagtag caatcagttt cacttttcct tcataggtac agatgtcacc agctgcataa
    25861 acgcctggaa tagaagtaga catatctgat tttacagtaa tggcatttct ggtactatca
    25921 agtccccaag aagaaagatg ttctaaatta gaagagaatc cataattgac gatcagagaa
    25981 tcaatcatca aatcaatcgt ctcgtcactt ttcacttttt ttaaccggat atctgtcagt
    26041 tccccattat tgcctgataa tccatcgatg agatagggag taagcaagtt gacagatgaa
    26101 gatttcaagc gtgatacact atgttcgtgc gcacgaaatt ccgggcgacg atgaatcaag
    26161 taaacttcgc ttgcaatagg ctctaacatc aacgtccaat cgatagcaga atcaccacct
    26221 ccagcaatag cgactttttt tccagcatac ttcatcaaat cattcacgaa ataatccaat
    26281 ccgtggtttt caaatgattc agcattatct aaattcaatt ttcttggctg gaatgaacca
    26341 tttcctaaag ctaaaatgac tgctttggaa taatggatgc ctttatttgt agtaatctcg
    26401 ataacttcgt cttcacgagt aagcgagaga acttcttctt ttaaatggaa tgtatggtta
    26461 aaagtagtta attgtttttc taattgatcg attaattcac ttgcttttat tgctggatac
    26521 ccaggaatat cgtaaatata tttttcggga tacaaagttg ctaattgacc accaagttga
    26581 ggtagactgt caatgatttt tgttttagct tggcgcattc ctgcgtaaaa agcggcaaac
    26641 atccctacag gacctgcacc gatgattgta atgtcataga tttccataaa aattcctccc
    26701 ttttcacact atagcagaat ttaattgaga atgcttctca aaaatgtttg aattcgaaat
    26761 cgaaacggta agttatagaa tgtatctagt gatgaaaaat aaaagaaaac aaatctagta
    26821 tttttacgag aggttaggta ttatgttatt attattatga agtatgcaaa tattaggagg
    26881 aaacaatatg gcattcccac aactagattt aaaaagtgaa aaaggaccaa aagctgttat
    26941 caaaacaaac cgtggtgaca ttactgttca attattccct gaactagcac cgaagacggt
    27001 tcaaaatttc attgaacttt ccaaaaaagg ttattatgat ggcgtgattt ttcaccgcgt
    27061 tattcctgat ttcatgatcc aaggcggcga tccaactgga acaggaatgg gtggcgaaag
    27121 tatttatggc gaaagttttg aagatgaatt cagtcgtgaa ctgttcaacc ttcgcggagc
    27181 tttatctatg gcaaattcag gtccaaacac aaacggaagc caattcttta ttgtaaataa
    27241 cacaaatgtg ccagctaata tgcttggaca actagaaggt gctggattcc catcagaaat
    27301 cattgaagcg tataaaggtg gcggaactcc ttggctagat ttccgacata cagtctttgg
    27361 acatgtacta gaagggatgg acacagtaga tgaaatcgca aatgtacaac gtggtccaca
    27421 agaccgacct gtacatgatg tagtgattga aacaattgaa atttcagaat agtttggtat
    27481 ggtacggagt aattatgaaa aaggacgaaa cctagtaagg tctcgtcctt ttattttgaa
    27541 taaatgattt aatcgattgt ctctaaagca tacttgattc gattaagtcc ttccagtaaa
    27601 gtttcttctg gacaagcaat attcaagcgc atatgcccat gaccggaagg accaaacgtg
    27661 atgccaggat ttaatacgac ttttcctttc cgaacaagag tgttctccaa ttcttggtcg
    27721 tctgaaatat aggcggtaaa atctaaccac attaagtaag ttccttcagg gttcattatt
    27781 ctcactttcg gaagatactg ttcaaaaaac tgattaactg tttgaacatt tttttctaga
    27841 taaggaatca gttggcttaa ccattctcca cccgtctcat aagccgcttg ggttccaatc
    27901 agtccaaaag tgttgatttc gtgctgttgg ttcatcacta aatgtttttc aaaatcagct
    27961 ttcatctctg gatttttgat aaataccatt gaatttttga ttgcagctag attgaaagtc
    28021 ttggttgcgg aagtaaaagc aactagtaga tcatctaaat taggatcaat cgttagcatg
    28081 gaagtaaatg tatgatcgaa taaagttaaa tcttgatgga tctcgtcact aaacagaatc
    28141 acttgatgtt ttaaacacaa ttcgcttaac tgtttcaatt cttctttgct ccaaacacgc
    28201 cctccaggat tgtgaggatt gcataaaatc attgctttta cattgttttc ttcgattttt
    28261 ttctccatgt cagacaagtc catgatgaaa tgattatttt tttctagcaa agagcttcga
    28321 acgatgtgac gttgatttgt ttctataata gaggaaaatg gaggataaac tgggtcatga
    28381 atcaaaatag aatcgcctgg ttttgtaaaa gtttgtactg ctgcagctaa actggttaac
    28441 acaccacttg taaaaacgat attttctttc gtcaactcca cagcatggtg ttggcgttcc
    28501 caatgaatga tagcctgata tagtttatca gacagagtgg cataaccaaa gattccatgt
    28561 ttgatgtatt cactaaacgc tttagctacc ccaccgggag acaagaagtc catatctgct
    28621 acccacaaag gaagtaagtc atccatttga tatgtctcag caattgcgtc ccattttaca
    28681 ctttctgtat cttttcttga aatctgttta tcaaattcga tcatcttaag acctccagtc
    28741 attttcgtcg cttctttttt tgagctattt acagtataat gataaatgcg aagagaggga
    28801 agtagatgac ttataaaatt ggacaaataa ttgaaggaaa agtgacaggg atacagcctt
    28861 atggcgcatt cgtttctctt gataacgaga cgcaaggact tattcatgtt tcagaagtac
    28921 aatccggtta tacgaaaaat atccattcat tgttaaaagt cggacagcca gtaacagttc
    28981 agatcatcga cattgatgaa tattccaaaa aaatcagttt atctttaaga acgttagaaa
    29041 aaacaaatcc agctgttcca tatcgcagaa aaaggtattt cacgaataaa aatcgtaaga
    29101 tcggatttac cactattgcg gagcagttac ctatttggat agaagaagca ttatctgaac
    29161 tagaacggaa agaaaataag aaataaaaag tgtatttttt ctgaaaatgt gctagaattt
    29221 ttatgtaagc catagaacaa gttgatcgag gtggaaattt tggcaagcaa aagagttgct
    29281 ggtacaatta tgttgcattt ggaggatggt actaaaaaat ttttggtcca ttcaattgat
    29341 gacaagttgg aatttgcgtt agcggaactt tctgaaggaa agactggttt agcgaatata
    29401 cttaatttct taaaagaaat agttcatatc gatgttagca agattagttt gatggaattg
    29461 acaaatacac atattaaaga ggaaaatgtt ccgttgtttg tctttgaaac tgaacaaaag
    29521 aatcaaagag aagaattagg cgaaggatat atttgggaag aaccaggtca aatgcgctct
    29581 gttcttggaa cttacgaagt agagggagtg cctttcttct aaaatacaga ttaagcgaac
    29641 aaagaagttt gaattttttt caaacttctt tttttattgt ttggtccaaa aaatacaatg
    29701 aaatgttctg ctaaagatag ttttttagtg cattttattt tcaaaatatg ttgaaaacgg
    29761 ttgtataacg aataataata attgatcgaa aaatcatttc acaaagtaat gcttaattta
    29821 tagttgacca aggaagaaat acatgataaa tttaacagcg ttgattctga agtggatgtt
    29881 atatcacatt tctaaaaaat ttaaaaaata gttattgaca tgttactaac gacatgataa
    29941 gataactgag tcgttaaaaa agaaccactt gcgaagcttg attctatcga aaaaaacttc
    30001 aaaaaagttc ttgacaaata acaaacgata agttatgata aatgagttgc tgaaagattt
    30061 cgaaaaacga aatcgaaaaa aacttttaaa aaagagttga caatcgcttg tctctctgat
    30121 atgatataaa agttgctacg gcagccaaaa atgaatcggg aaacactct
//
LOCUS       DACEQH010000034        27388 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.034, whole genome shotgun sequence.
ACCESSION   DACEQH010000034 DACEQH010000000
VERSION     DACEQH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.034"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            161..391
                     /locus_tag="IW833_001580"
     CDS             161..391
                     /locus_tag="IW833_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ1307705.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="IW833_001581"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="IW833_001581"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1307706.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="IW833_001582"
     CDS             1802..1978
                     /locus_tag="IW833_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1307707.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="IW833_001583"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="IW833_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ1307708.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="IW833_001584"
     CDS             2593..3060
                     /locus_tag="IW833_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1307709.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="IW833_001585"
     CDS             3145..4029
                     /locus_tag="IW833_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1307710.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4146..4379
                     /locus_tag="IW833_001586"
     CDS             4146..4379
                     /locus_tag="IW833_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ1307711.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="IW833_001587"
     tRNA            4467..4547
                     /locus_tag="IW833_001587"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="IW833_001588"
     tRNA            4554..4625
                     /locus_tag="IW833_001588"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
     gene            5140..5847
                     /locus_tag="IW833_001589"
     CDS             5140..5847
                     /locus_tag="IW833_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1307712.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="IW833_001590"
     CDS             complement(5907..6551)
                     /locus_tag="IW833_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ1307713.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6678..6965
                     /locus_tag="IW833_001591"
     CDS             6678..6965
                     /locus_tag="IW833_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307714.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7073..7393
                     /locus_tag="IW833_001592"
     CDS             7073..7393
                     /locus_tag="IW833_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1307715.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7474..8787
                     /locus_tag="IW833_001593"
     CDS             7474..8787
                     /locus_tag="IW833_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1307716.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8784..9503
                     /locus_tag="IW833_001594"
     CDS             8784..9503
                     /locus_tag="IW833_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ1307717.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9631..10182
                     /gene="raiA"
                     /locus_tag="IW833_001595"
     CDS             9631..10182
                     /gene="raiA"
                     /locus_tag="IW833_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ1307718.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10404..12938
                     /gene="secA"
                     /locus_tag="IW833_001596"
     CDS             10404..12938
                     /gene="secA"
                     /locus_tag="IW833_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ1307719.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13064..14165
                     /gene="prfB"
                     /locus_tag="IW833_001597"
     CDS             join(13064..13126,13128..14165)
                     /gene="prfB"
                     /locus_tag="IW833_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ1307720.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEKEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14270..14956
                     /gene="ftsE"
                     /locus_tag="IW833_001598"
     CDS             14270..14956
                     /gene="ftsE"
                     /locus_tag="IW833_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ1307721.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14940..15833
                     /locus_tag="IW833_001599"
     CDS             14940..15833
                     /locus_tag="IW833_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1307722.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15985..17433
                     /gene="cls"
                     /locus_tag="IW833_001600"
     CDS             15985..17433
                     /gene="cls"
                     /locus_tag="IW833_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1307723.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17770..18627
                     /locus_tag="IW833_001601"
     CDS             17770..18627
                     /locus_tag="IW833_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ1307724.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18742..19662
                     /gene="pstC"
                     /locus_tag="IW833_001602"
     CDS             18742..19662
                     /gene="pstC"
                     /locus_tag="IW833_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ1307725.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19662..20546
                     /gene="pstA"
                     /locus_tag="IW833_001603"
     CDS             19662..20546
                     /gene="pstA"
                     /locus_tag="IW833_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ1307726.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20561..21367
                     /gene="pstB"
                     /locus_tag="IW833_001604"
     CDS             20561..21367
                     /gene="pstB"
                     /locus_tag="IW833_001604"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1307727.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21383..22141
                     /locus_tag="IW833_001605"
     CDS             21383..22141
                     /locus_tag="IW833_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1307728.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22154..22831
                     /gene="phoU"
                     /locus_tag="IW833_001606"
     CDS             22154..22831
                     /gene="phoU"
                     /locus_tag="IW833_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ1307729.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23054..24085
                     /locus_tag="IW833_001607"
     CDS             23054..24085
                     /locus_tag="IW833_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1307730.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24082..24849
                     /locus_tag="IW833_001608"
     CDS             24082..24849
                     /locus_tag="IW833_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1307731.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            24949..26517
                     /gene="liaX"
                     /locus_tag="IW833_001609"
     CDS             24949..26517
                     /gene="liaX"
                     /locus_tag="IW833_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ1307732.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26696..27001
                     /locus_tag="IW833_001610"
     CDS             26696..27001
                     /locus_tag="IW833_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ1307733.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF"
     gene            27001..27351
                     /locus_tag="IW833_001611"
     CDS             27001..27351
                     /locus_tag="IW833_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ1307734.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
BASE COUNT         9035 a         4646 c         5922 g         7785 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4741 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4801 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtc
     4861 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4921 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4981 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     5041 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     5101 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5161 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5221 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5281 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5341 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5401 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5461 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5521 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5581 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5641 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5701 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5761 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5821 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6601 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6661 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6721 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6781 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6841 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6901 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6961 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     7021 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     7081 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7141 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7201 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7261 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7321 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7381 cataggtgaa taaatagaac agtataacta tcgaacaagc gatccaaaaa atagatcgct
     7441 tgttttttcg tatatttttt tagggcgaat aatatgaatc atttttgggg tagaaggctt
     7501 ctgacgagag atattaaagc aatagactgt gaggaggtaa atttaccaaa ttatgagaag
     7561 atcgcagcag tggaacgttt gggcaactgg attctttgtc atcgctgtgg aatgaggacg
     7621 tccgtcattg atggacagct tgcagattac ggttattttt gtacacattg tctatattta
     7681 gggcgatgcg acactcaaca agatttatat ttgtttgatc agcctgaagc aaaacctcga
     7741 gaagtcgtct ttttttggac aggacagctt actgaaaaac aaacggaaat cgcaaagaga
     7801 atactttgcc attcagaaaa aagacatcat cttatttggg ctgtaacagg tgcagggaaa
     7861 acagagatgc tttatcctat tttagtggaa actttaaaag ctggaggaag agtagcgatt
     7921 tgtacaccgc gagtcgatgt ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca
     7981 gaagaaaaaa tcacgttgct ccatgggaat acagcaacgg cttatacctt tacttctttt
     8041 gttatctgta cgattcatca gctataccgt ttttatcggt cctttgattt aatagtgatc
     8101 gatgagatcg atgcgtttcc ttataacggg gatgcaggat tagctcatgc tgtgcaaaca
     8161 gcgattaaac cagacggacg attcatctac ttaagtgcca cacctgataa aaagttacta
     8221 aaggaaatca agcagacgtt tgacatccac aaacttgctt tgcgctttca tcggcgttta
     8281 cttcctgaac ccgtcttggt tttcatgaat aattggcgac aaaaatgcaa gaacaagaag
     8341 aagataaaat ctttggtgcg gatcattcat caattaatta aagaaaacca tatcctttta
     8401 ttttgtccaa gtatcacgat gatgcatcgt ttgaaagcca cacttcaaga ggctcttccg
     8461 agatatgaga tcacagatgt ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa
     8521 atgcgagaag caaaatatga catactgtta accacgatga tccttgaacg tggagtcaca
     8581 tttgaaagaa tatccgttat cgttttagga gcggatcatc cggtttttac taaatcttct
     8641 ttggtacaaa tcgcaggaag agccgatcgt aaagggcctt atacgaacag tcaagtatac
     8701 tttttctatg aagaaaaaac tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg
     8761 aatgaagaag ggaagaagat gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa
     8821 atttaacatt cagcgagatt attggtgttt cgaaatacag attacctcag acttgttcag
     8881 tttgtcgcgc acaatttcat ttattggaaa caagaggctg ttctggctgt caaaaaccaa
     8941 tccatttcaa tctagagagt acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg
     9001 actgtcaaga atggaagaag aaatatccga cctatgattt tcagcaccgt gccttttttg
     9061 tgtacgatga aaatatgaag gcctggctca atcaatacaa gtttatggga gatatccgac
     9121 tagcaggcac ctttgcatat aaatggacgc agataaaaaa agaatacccg acatacatct
     9181 attgtccaat acctttatct gaaaaaaggc gaacagaaag aggattcaat caagtaagcg
     9241 agatgttgaa agcagcaaga attccttttc agatgctact caaaagagag aagcatctgc
     9301 tggctcaagc aaaaaacacg cgaaaggaaa gattggagat gcctcaacct tttgaacttg
     9361 cagtcacctt ttctaagata caaaataaaa aaatattgat tattgatgat gtctatacaa
     9421 caggacagac tcttttccac gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc
     9481 gaaccatttc tttggcgaga tgaattagat gtggaaattt gatagcgatt taatttgcaa
     9541 ataacctaga gttcgatata atagaattaa gaagagggaa agagtggtcc ttctccttct
     9601 tgattgtggt agacgaaagg ggtaattttt atgtttagat ataatgtacg cggcgaaaat
     9661 atcgaagtaa ctgaagcgat tcgcgactac gttgagaaaa aagtaggcaa actagaacgc
     9721 tatttcagtg acgcacccga tgcaacggcg catgtgaact taaaagttta tacagaaaaa
     9781 acagcaaaag tggaagtaac gatcccatta ccttatcttg tattacgtgc cgaagaaact
     9841 tctccagatt tatacgcaag tatcgatttg gttgttgata aattggaacg ccaaatccgc
     9901 aaattcaaaa caaaaattaa tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct
     9961 gctgctttat tcaatgaaga ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt
    10021 acgaaacgcc tttcattgaa accaatggat agtgaagaag cagtcttaca aatgaacatg
    10081 ctgggacata acttctttat ttttgaagat gcagaaacaa atggtacaag tatcgtttac
    10141 cgccgtaaag atggaaaata cggactgatt gaaacagact gatcagcctt acttaaataa
    10201 caacgaaaac ttgcagcagc tactgacagc tgccgcaagt ttttttgagt ttcctcctct
    10261 tttagatgaa atatgaataa attatgaagt tcttacttta atacgcttaa gatatttcaa
    10321 ttatctataa ctaatgataa aatgaaaagt aaagtctaca tagtctggat aatacacaga
    10381 aggaaaagga gattacacaa cgcatggcca atttgattcg ttcacttatc gaaaacgata
    10441 agaaagaact aaaacgttta gatagcattg caaaaaaagt tgaagcatac gctgaccaaa
    10501 tggcagcatt aactgatgaa caattacagg caaaaactgc tgaattcaag ggcagatacc
    10561 aaaaaggaga aacattagat caattacttc cggaagcttt tgcagtcgtc cgagaagcag
    10621 caaaacgtgt attggggtta tatccatacc acgtacaatt gatgggtgga atcgtgcttc
    10681 acgatggaaa tatccctgaa atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc
    10741 cagtatatct aaatgctctg actggcgaag gggtacacgt agttaccgtc aatgaatatt
    10801 tagcaacccg tgactcgacg gaaatgggcg aactttataa tttcttagga ttaacagtgg
    10861 ggttgaatat caactcaaaa tcttcggatg aaaaacgcga ggcttataat tgtgatatca
    10921 cttatagtac gaataatgaa ttaggatttg actacttgag agacaatatg gtcgtttacc
    10981 gccatcaaat ggtacaacgt ccgctgaatt atgccattgt cgatgaagtt gactcaatct
    11041 tgattgatga agcgagaacg ccgctgatca tttcagggca agcagaaaaa tccacagctt
    11101 tatatacaag agcagacaac ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg
    11161 atgtacaatc aaaaacaatc agtttaaccg aagcagggat cgaaaaagca gaagaaacat
    11221 tcggtttgga taacttatac gacattgaaa atactgcatt gacccatcac ttggatcaag
    11281 cattgagagc caactatatc atgcttttag atatcgatta tgttgtccaa gacggaaaag
    11341 tactgattgt tgaccagttc actggtcgga tcatggatgg acgccgttat tcagatggac
    11401 ttcaccaagc aatcgaagct aaagaaggcg tagagatcga agacgaaacg aaaaccatgg
    11461 cgacgatcac tttccagaac tatttccgta tgtacaagaa acttgcggga atgactggta
    11521 cagctaaaac agaagaagaa gaattcagag aaatttataa catccaagta taccagattc
    11581 cgacaaatcg tccggttatc cgtgatgatc gtccagattt actttaccca acattacaaa
    11641 gcaagttcca cgctgtcgtg caagatatca aagatcgcta ccgcaaaggt caacctgtgt
    11701 tagtcggaac tgttgcggtt gaaacatctg agttattatc agatatgctg aatcaagaac
    11761 gtattcctca tgaagtgctg aatgcgaaaa atcactttaa agaagcagag atcatcatga
    11821 atgctggtca aaaaggtgct gtgacgattg ctaccaacat ggctggtcgg ggaacagata
    11881 tcaagttagg acttggggta agagaacttg gtggtctagc agttatcggg acagaacgac
    11941 atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg
    12001 gtatgtcaca attctacttg tcattggaag atgatttgat gaaacgattc ggttcagaac
    12061 gaatcaaagc tttcttagat cggatgaaga tcggtgagga agatgctgtt atccaaagca
    12121 agatgctgac gaaacaagta gaatctgcac aaaaacgtgt agaaggaaat aactatgaca
    12181 cacgtaaaaa cgtcttacaa tacgatgatg tgatgcgtga acaacgtgaa gtaatctata
    12241 aacagcgaca agaagtaatc atggaagaaa aaagcttatc caagccacta atgaacatgg
    12301 tcaaacggac aatcagtcgt gtagtagatg cgcatacaca attagagaaa gaaaattgga
    12361 atttggaaag tatcatcgat tttgccggca gtactcttgt ccatgaagat agtattactc
    12421 ttgcagatat cgaaggaaaa acatctcagg agatcaaaga gtatctaaac gaacgagcac
    12481 aagaagtatt taataccaaa gcggctcaac taaatggtcc agagcaactg ttggaatttg
    12541 aaaaagtcgt gatcttgcgt gttgtggact ctaaatggac agaccatatt gatgcaatgg
    12601 atcaattacg tcagtcaatc ggcttacgtg cttatggaca gaacaatcca ttagtcgaat
    12661 atcaaacaga aggctataag atgtttgaag atatggtagg cgccatcgag tatgaagtca
    12721 ctcgaatctt tatgaaagct gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag
    12781 gaacagcttc tcactcagaa gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa
    12841 aacagccaat ccatgtgaac aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga
    12901 aaaaatataa gaactgtcat ggaaaaaatg caaactaatt tctgacagca ggattggtga
    12961 gacaaattct ttttttaaga attcgtctca ccttctttct gttatttgct gattctgtta
    13021 gactagatat aatgaaaaaa gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat
    13081 tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagt ttcaggaggt ctctttgact
    13141 tagatcagct ggaagaagac atcgcggaag cagaagcaaa aatggctgaa cctggttttt
    13201 gggatgacag cgaagccgca caaaaagtga tcagcgaaaa taacgctaat aaagaaacct
    13261 atgaccaatt ccattctctt gccgaagaac tagaagaaat cgaattgatg gtggagatgc
    13321 tgcaagaaga acctgatgca gcaatgcaag aagaagcaga aacacgtatc aatgcattag
    13381 atgaaaaaat gaagacgtat gaattatcta tgctgttaga cggaccttat gatcgaaaca
    13441 atgcgatcgt cgaattgcat ccaggggctg gcggaacgga gtcccaagac tggggcagca
    13501 tgcttttacg tatgtataca cgttgggcag agcagcatgg ctatcaagta gaaacactag
    13561 attatcaagc aggtgatgaa gcagggatca agagtgtgac cctgctgatt aaaggacata
    13621 acgcttttgg ttatctaaaa tcagaaaaag gtgttcatcg ccttgtacgg atttcgcctt
    13681 ttgactcagc gaaacgacgt catacttctt tttgttcggt agatgtgatg ccagagcttg
    13741 atgaaacgat cgatatagaa atcaatccag atgatttgaa aatcgatacg taccgagcaa
    13801 gtggtgcagg tggtcagcac atcaataaaa cagaatctgc tgtacggatc actcacttgc
    13861 ctacaggaac agtagtagct agtcaggcac aacgctcaca attgaaaaac cgtgagcaag
    13921 cgatggggat gttaaaagct aagctttatc aattagaagt ggaaaagaaa gaaaaagaag
    13981 cagctgcact tcgaggagaa caaaaagaaa tcggctgggg ttcacagatc cgttcttatg
    14041 ttttccatcc ctattctatg gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc
    14101 aagcagttat ggacggtgac cttgatttat ttattgacgg ctatttaaaa atgcatctac
    14161 aataagaact cattgttaaa ttgaaataaa attgtaagat tttgcaagca tgttgaaaca
    14221 ttgccatgct ataatagcta aagactgaaa aagatatgga gagaacgcca tgattgaaat
    14281 gaaggatgta atgaagaagt actcaaatgg tacgaccgct atccgcaatc tctcgattgt
    14341 catcgaacaa ggagagtttg tctatgtggt tggtccgtct ggagccggta aatctacatt
    14401 catcaaattg atgtatcgag aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca
    14461 tgatctaata gcaatcaaaa accgcgaagt tccttatcta cgcagagaaa tcggcgtggt
    14521 gttccaagac tataaattat taccaagaaa aacagtatat gaaaatgttg cgtacgctat
    14581 gcaagttatc ggcagaaaac ctcgagatat caaaaaacga gtaatggaag tgctggacct
    14641 tgttggtttg aaacacaaag ttcgagtctt tcctagcgaa ttatccggtg gggaacagca
    14701 acgtgtctct attgctcggg caatcgtgaa tacgcctaaa gtacttatcg cggatgaacc
    14761 gacaggaaat ctggatccag aaaactcatg ggaaatcatg aaattattag atcggatcaa
    14821 tgcccaagga actactgtcg tgatggctac acataacagt acgatcgtaa atacgattcg
    14881 gcaccgagta attgctatcg aaaacggccg cattattcga gatcaagcgg aaggggaata
    14941 tggatacgat gattagaacc tttttccgcc atttattaga aagtatcaag agtttgaaac
    15001 gtaacggctg gatgacagtt gcttcagtca gtgcggtaac gatcacgttg actttagtcg
    15061 gtgtattcat ggcagttatc atgaatgcaa ctaaattagc tcaggatata gaaggaaacg
    15121 ttgatgtatc tgtattcgta gatatcggta caaaagacga agagatgaaa aagctagaaa
    15181 aagaattgaa tgacttagat catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat
    15241 tgaaaaaaat ccaaaacgaa atgggtgatt cctggaaatt gtttgaaggt gacaataacc
    15301 cgttatatga tgtgtatgta gtaagtgcta ctgatccttc ttatacaaaa caagtagcag
    15361 aagaagcagc agatttgaag aatgtttctc gttctgatta cggtggagct tcctcagatc
    15421 ggattttcca aatcgctggt gcagttcgga catggggatt agttgcagcg gcattgctgc
    15481 tgttcgtagc gatgtttctc atttcgaaca cgattcggat cacgattttg tctagacagc
    15541 gtgaaattca aatcatgcgt ttagtcggtg caaaaaatgg atatattcgc tggccgtttt
    15601 tccttgaagg tggctggatc ggtctgatcg gtgcgattct tccgattttg atcatcacat
    15661 ttggttatgc atggtttttc caattgatca atccatcatt attacgttcc cattattcat
    15721 tgattcgtcc acaaaatatc gtttggaaaa tcaatctttt aatggtcggc attggtgtga
    15781 tcattggttc acttggctcg atcatttcta tgagacgttt tttgaaattc taataccaca
    15841 atataaataa aatgagaaag ttggacagtt tcgttccagc tttctttttt tattggctag
    15901 gagaatagaa ggaagcggct tttttccctt tactttaaaa aacaacttgg gtattcttaa
    15961 gttggtagaa gaagaggtga agccgtgaaa attatcgttg acaatttttt tacgatcctt
    16021 ttgattatga atattttgct ttcctttatc atcgtttttc gagaaagaaa ggaaactgct
    16081 caaacatggg catggctgct cgttttgatg ttcattccag ttgtaggatt tattttatat
    16141 atttttttag gaagagggat ttccaaagag aaaatctttg atttgaagat ccaagggaaa
    16201 atcggtaaga acctggaaat tgaagaagac aaacaggcat tgatgcgagg gctttatcct
    16261 catccgccta ctggacaagt agatgtgaaa caattgatct atatgctcac ggtatttgaa
    16321 agtacgctct atacgactaa aaatgaaatc actttattta cagatggtcg tgaaaaattc
    16381 gatgcgctga ttgaagatat caagcaggca gaagaccata tccacttcca gtattatatt
    16441 tatcgcagtg atgcattagg tgaagaagtt cgagatgcat tgacagaggc ggcaagacga
    16501 ggggtaaaag tcagagtcct tcttgatgca tggggttcca cacaagtttc ttccagcttt
    16561 tttcagaatt tgaaaaaagc aggcggagaa gtcgcatttt tctttcctct atttgttccg
    16621 tatattaatc ctcggatcaa ttatcgcaat catcgtaaaa tcgtagttat tgatgggaaa
    16681 atcggatata caggtgggtt caatgttgga aatgaatatc taggattggt aaagaaattt
    16741 ggttattggc gagacaatca tttgcgtatt tacggagaag cggtgtatac cttgcaaaac
    16801 cgttttttga tggactggaa ttctcagcat acaaaagaag taggctattc tcccaaattt
    16861 ttcccgtcca ttcacactac tggtgatata gctgttcaaa tcgtgacaag cggaccagat
    16921 accgaacatg aacaaatcaa aatgacttat ttgaaaatga tttcgttagc caaacgggag
    16981 atattgatcc aaacgcctta ttacataccg gatggatcga ttcatgaagc actgaagttg
    17041 gctcttcttt caggcgtaaa agttcatatc caaataccta acaaaccaga tcatcttctc
    17101 gtttactggg caacgtattc tttcgctgca gagctgatcg agtatggagc aaggatcgaa
    17161 acatatgaga atggatttat tcatgcaaag acgatgatta tcgatggtgg cattgtttca
    17221 gttggctcag caaatatcga tgttcgttca tttcgcttgg actttgaggt caatacactg
    17281 gtttatgatg aacgaatggc ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt
    17341 tctacgcatc tcacaaaaga aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa
    17401 ggattagcaa gattgatttc gccgctcctt taaaacataa aaaggacatg agaaccatca
    17461 acagatggag atctcgtgtc cttttctaac ggggccattt ctgagagtac gtcagaaatg
    17521 gagaaagcgt ctcgcatgag cgaaattaac tttggggtat ctcttgtttt ttggggaaag
    17581 caagaaataa gctaatatat gctattaaaa gagacacatt tcaaagccag tatagcatag
    17641 aaacttttaa tgagatagtt ttttttacaa ttatgagaat ttattttgtt atttaatgat
    17701 tttgattcgt tttcatcaag taatgaaaat gataaaataa acatacttta ggattaaagg
    17761 agatttctaa tgaaaaaact taaattattc ttaccaataa taggattagc cattctttta
    17821 tcaggctgtt caaagtggat tgatcgagga gaatccatta cggctgttgg ctcttctgct
    17881 ttacagcctt tagtagaaac agttgctgaa acttaccaga cagaaaatcc tggaaaattc
    17941 atcaatgtac aagggggcgg aagcggaact ggtttgtctc aagtacagtc aggagcagtc
    18001 gacatcggaa actctgattt gtttgcggaa gaaaaatcgg ggatcgatgc ctcagcatta
    18061 gtcgatcatc gagtagcagt tgttgggatc actcctattg tgaacaagga tattggcgta
    18121 aaagacatca gtatggaaaa cctgaaaaaa ctatttacag gcgagatcaa aaactggagt
    18181 gaagtcggtg gaaaagatca gccagtagtt atattgaacc gcgcttcagg aagcggaacc
    18241 cgtagcacgt ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa
    18301 gattcaagcg gaatggtgcg ccagatcgtt gccgacacac ccggtgcaat cagctatgtt
    18361 gctttttctt atgtttccga tgatgtagat acgttgaatg ttaacggtgt agaaccgaca
    18421 gatgaaaatg tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga
    18481 gatcatccaa aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa
    18541 gataacatcg ttggagaact aggttatatc cctgtttctc atatgaaagt cgaacgtgat
    18601 tggcaaggaa atatcgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact
    18661 tttacatgtt attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac
    18721 ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa
    18781 cgttcaaaaa tggaacagcg tggaaaattc atcagttttt tatgtatcgc gctgatcgtg
    18841 ttggttgtat tatctatttt ttattttgta gctagtaaag gattggccac cttttttact
    18901 gataagatca atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatcgga
    18961 gcaaatggga aaccaatggt aggggcattg cctatgatca caggttcgtt tattgtgacc
    19021 tttttatctg ctatcgttgc cacgccattt gccattggtg cagcagtatt catgacggag
    19081 atctctccta aaaacggaac gaagatctta cagccggtca tcgagctgct tgtaggtatt
    19141 ccttctgtcg tttatggttt tatcggttta tcagttatcg tgccttttgt cagaagtatt
    19201 tttggcggga cagggtttgg tatcttagct ggtacatttg tgttgttcgt tatgatcttg
    19261 ccgactgtca caacgatgac tgttgatgca ttaaaggcag taccacggca ctatcgggaa
    19321 gcttcattag cgcttggtgc cacaagatgg caaacaatct acaaagtcgt gctacgagca
    19381 gccgtgcccg ggattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggtgaagcc
    19441 ttagctatcc aaatggtcat cggtaatgca gcattgatgc caacaggact tactactcct
    19501 gcttctacat tgacgtccat cttaacaatg ggaatcggaa atacaatcat gggaacagtt
    19561 gaaaataatg tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat
    19621 atcgtgatcc gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag
    19681 ataaaattgc tacaggtgtt ttatatatag tttcggggat catcgtattg attttagcag
    19741 ctttactact ttatatctta gttcggggaa taccgcacat ttcatggtcc ttcttgaccg
    19801 aaccttccaa agcctaccaa gaaggtggtg gaatcgggat ccagctattt aactcgattt
    19861 atttgctttt gatcacgatg atcatcagtt ttcctatttc tttaggtgcg ggtatctatc
    19921 tttcagaata tgctggaaaa aactggctaa cagatgtgat tcgcacgtcg attgaaatct
    19981 tgagctcgtt gccttctgtc gttgtcggtt tattcggttt cttggttttc gttattcaat
    20041 tccagtatgg tttctcgatt atttctggtg cattggcttt gactgtattc aacttgcctc
    20101 ttttgactag aacagttgag gagtctttac aagcggtcca ttacacacaa cgagaagcag
    20161 gattggcctt aggtttgtca cgctgggaaa cagtgatgaa agtggtggtt ccagaagcga
    20221 caccagggat tttgactggt gtgatcctaa gttctggaag aatttttggt gaagcagctg
    20281 cattgatcta tacggctgga caaagtgcac ctgcgttaga ttttacgaac tggaatccat
    20341 taagtgtttc aagcccgatc agcattttcc gtcaagctga aacattagct gtccatattt
    20401 ggaagatcaa tacagaaggc acgatgcctg atggtgtttc tgtttcggct ggcgcttctg
    20461 ccgtgttgat cattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat
    20521 tataccgaaa aatgacttca gcctaaggag aagaagcaaa atgaaaaaat ataactggaa
    20581 tgaaacaaat gtcatcacca tgccaaaaga gaaagaagta gcactatata cggatgatat
    20641 acatgtctgg tatggcgaca atgaagcaat caaaggagtg gatctggaat ttgaaaaaaa
    20701 taagatcact gcactgattg gtccttccgg ctgcggaaaa tctacgtatc ttcgttcgtt
    20761 gaatcgaatg aatgatggga tcgctaatac gaaaatcact gggaaaatca tgtatcaagg
    20821 cgtagatatt aatgcgcctc aagtggatgt ttatgagatg cgcaaacgga ttggcatggt
    20881 ttttcaacgt cctaatccgt tcagcaagtc gatttatgac aatattacat ttgctttgaa
    20941 acagcacggc gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca
    21001 agctgccttg tgggaccaag taaaagacag cttggacaaa agtgccttgg ctttatctgg
    21061 cgggcaacag caacgtttat gcattgctcg tgcgatcgcg atgaaacctg atatcttgct
    21121 gctagacgag ccggcaagtg ctttggaccc tatttcgaca ggaacggttg aagaaacact
    21181 tgtaaacttg aaagaagact atacgatcat catcgtgacg cataacatgc aacaagcagc
    21241 tcgtatcagc gactatactg cctttttcta tctaggaaaa gtaatggaat atgacaaaac
    21301 taacaagata tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt
    21361 tggttaagac ggaggcctca acatgacaaa agaaattatt tcatctaaag atcttcatct
    21421 gtattatgga aagaaagaag cactaaaagg gatcgatcta agtttcaatc aaggggaaat
    21481 cactgcgatg atcggtcctt ctggttgcgg aaaatccact tatctgcgtt ctttgaaccg
    21541 gatgaatgac ctgattccga atgtaacgat caccggaagt gttttatata aagacaaaga
    21601 catctatggt ccaaaaacag atgttgttga actaagaaaa gaaatcggca tggtattcca
    21661 gcagccaaac ccatttcctt tttctatcta tgaaaatgtc atctatggat tgaaattaaa
    21721 aggggaaaaa gataaaaaag tccttgatca agtagttgaa gaaagcctga aggcagcgtc
    21781 tgtttgggat gatgtgaaag acaaattgca taaaagtgct ttatctttat ccggcgggca
    21841 gcagcagcgt gtatgtatcg cacgtgtttt ggcagttaat ccggaaatca tcttattgga
    21901 tgaaccaaca agtgcgcttg atcctgtttc tactgggaaa atcgaaagta tgctgttaga
    21961 attaagagag cggtatacga tgatcatcgt gacccataat atgtctcaag cttcacgtat
    22021 ttcagataag acggcattct ttttagatgg tcacttgatc gaatacaacg atacgaagaa
    22081 gatattcatg aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata
    22141 aggaggaaac ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca
    22201 gttctacgaa atggggatga tggtgagcag cgccgtgcac aagtcagttc gtgcatacat
    22261 caaacacgat aaaatcttag cccaagaagt tatcgaaaac gatataaata tcaataacat
    22321 ggaaacacgc ttggagaaga aaagttttga aatgatcgca ttgcaacagc cagttacaac
    22381 tgatttgcgt atgatcatta cggtcatgaa agctagctcg gatttagaac gtatggccga
    22441 tcatgcggtg tctgttgcta aatccacgat tcgcgtaaaa gggcagacga gaataccgga
    22501 aatcgaaaaa gagatctccg atatgtccga ttatgtgaaa aaaatggtgg acaacgtcct
    22561 agttgcttat gtcaaaacag acgaaaaaga cgcacgtacg atcgccaata tggatcaaag
    22621 agtcaatgac tatttcaatc gaatctataa tgcaacaatc aaaaatatgc aggaaaatcc
    22681 tgagacagtc atcagtggaa cagactactt gaatgttgcc agttatctag aacggatcgg
    22741 agactatgtg acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga
    22801 gttaaatacg aaccacaatg agattttcta aactgaagct gtgattatgc cgtttgttta
    22861 tgaaaagagg ttgtgaaaaa gctgttctac atcaagtaat aagaacagtc aaacaaaaaa
    22921 tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc ttttgaacac
    22981 cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca tcatttcata
    23041 gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta tttgtgtgct
    23101 tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca tcgagataga
    23161 aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga actcggatca
    23221 aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag aaacagcaaa
    23281 tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc agctagtagc
    23341 aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg ccatgttgct
    23401 tattggcgga aaacagggtc tatttgcttt aggaagtctc atcatcaata tcgttttact
    23461 tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa ataaatctat
    23521 cctgttcgtc gttataggga tgattattag catattggct ttagatggat ggaatagaag
    23581 cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt ttatttgtct
    23641 tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga attatctgac
    23701 acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg gaggaacact
    23761 cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac aaccagaaat
    23821 aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga ttggctcgat
    23881 gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc ttttatattt
    23941 aggaaatcaa tggtctttca aagaaacgat agaacagcat ttatcattgg aatttttacg
    24001 cgtcattgct ggcagcttag gaatagtatt atcgatcccc atctcggtcc tcttttttct
    24061 gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat tttagcaatc
    24121 agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt attcatcaat
    24181 ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat actggcattg
    24241 tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa cggttataat
    24301 ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt cctactattg
    24361 cttcctatca ctgatttttt agcgatccaa ggttttacat cggtagaact agaagaatta
    24421 tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc gttgcttctg
    24481 attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg gacattcgaa
    24541 gtatatcagg caaatccgca attatctttt taccaattgc gccattctag tttcgctatt
    24601 gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat gggaagttct
    24661 ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt gatcaatgaa
    24721 aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc actaattgtt
    24781 cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga acaactcaag
    24841 atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt gcataatatg
    24901 gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat gaaagaaaga
    24961 gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg cttagattta
    25021 ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc agacaaggta
    25081 actgctagtc ataaagaaaa agataaggca agtcaattga tcgataaact tgaaaacggc
    25141 gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga tcaagaaaac
    25201 ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc gcgtctagat
    25261 gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca ggaagcattg
    25321 atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt agcacagcgt
    25381 caggcgttag aggcagaaat caaagagtta gaagctagtg gcgatacact gttagaagaa
    25441 caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc tgagaaaaaa
    25501 gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc aacagataca
    25561 ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa attcttgaag
    25621 aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa agatgtcagc
    25681 ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta tgaagctcct
    25741 gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa aacgtgggac
    25801 agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg tgctgaacca
    25861 tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat ctccttccaa
    25921 attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg agtatacgac
    25981 cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga agctaaagat
    26041 atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc aacgatgctg
    26101 gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt agattctatt
    26161 atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaatct ttctgtgtct
    26221 ttagtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa agtagaagca
    26281 agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt ggaaggacat
    26341 gctaaaacaa gtcttggaag tatcaatagc cgtctgtcca attacgaagt agttcgtgag
    26401 aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatga tgagatcgcg
    26461 caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga taaataaaga
    26521 ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa atagcttccc
    26581 ctattttaca aaattgtgcc ttattgccga taggctatct cttgaacacc gattaagaag
    26641 aaggttgtca caacgatttt cttcatttaa aatagtagaa cagaggtgag acatgatgaa
    26701 aaaaaagttg acaaaatcca ataaaaatgt tgttttgaca ggtactttag cagggattgg
    26761 ggaatatatc gggattgatc ccactgttat ccgtgtgctt tatgtattcc taagcttagt
    26821 tgcttttgga agtccaatca ttttatacat tttattagct ttgattatcc catctgcgcg
    26881 ttctgcacga acaaactatg gacataacaa tgcatattat gaaaaaaaca attatcgtga
    26941 acaaacaaaa aaacgcaaag aagcggaaaa aatcgatgat gacgaatgga gtgactttta
    27001 atgtcgtact ttcagcgtat tttggttaat acgttaacat tcatatcgtt ggctgttctt
    27061 tttcctgcga tggtccatgt gagcagtatt tggacagccg tccttgccag cttcgtatta
    27121 tctgtgttga acatgtttgt caaaccgatt ctgacgatcc tgtccctgcc ctttacatta
    27181 gtcacattgg gattgtttac gtttgttatc aatgcagcga ttcttaaaat gacttccttt
    27241 tttgtaggag cagaacattt tagtttttct tcatttggtg cagcgttatt cgtagctatc
    27301 ttgatgtcac ttgtcaatgt aatcgttagt gaacataaca gcagaaagta aaaaaagctg
    27361 tgccgagatc tcggcgcagc ttttttgt
//
LOCUS       DACEQH010000035        26027 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.035, whole genome shotgun sequence.
ACCESSION   DACEQH010000035 DACEQH010000000
VERSION     DACEQH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.035"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="IW833_001612"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="IW833_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ1307735.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="IW833_001613"
     CDS             808..1242
                     /locus_tag="IW833_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ1307736.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="IW833_001614"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="IW833_001614"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ1307737.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3465..5915
                     /gene="parC"
                     /locus_tag="IW833_001615"
     CDS             3465..5915
                     /gene="parC"
                     /locus_tag="IW833_001615"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ1307738.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6192..8423
                     /gene="pflB"
                     /locus_tag="IW833_001616"
     CDS             6192..8423
                     /gene="pflB"
                     /locus_tag="IW833_001616"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ1307739.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8667..9428
                     /gene="pflA"
                     /locus_tag="IW833_001617"
     CDS             8667..9428
                     /gene="pflA"
                     /locus_tag="IW833_001617"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ1307740.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9513..10439
                     /locus_tag="IW833_001618"
     CDS             9513..10439
                     /locus_tag="IW833_001618"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ1307741.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10621..11514
                     /locus_tag="IW833_001619"
     CDS             10621..11514
                     /locus_tag="IW833_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ1307742.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11648..11773
                     /locus_tag="IW833_001620"
     CDS             11648..11773
                     /locus_tag="IW833_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1307743.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11907..12539)
                     /locus_tag="IW833_001621"
     CDS             complement(11907..12539)
                     /locus_tag="IW833_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307744.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12687..13229)
                     /locus_tag="IW833_001622"
     CDS             complement(12687..13229)
                     /locus_tag="IW833_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ1307745.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13364..13753
                     /locus_tag="IW833_001623"
     CDS             13364..13753
                     /locus_tag="IW833_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307746.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13757..14521
                     /locus_tag="IW833_001624"
     CDS             13757..14521
                     /locus_tag="IW833_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1307747.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14650..15597
                     /locus_tag="IW833_001625"
     CDS             14650..15597
                     /locus_tag="IW833_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1307748.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15727..16467
                     /locus_tag="IW833_001626"
     CDS             15727..16467
                     /locus_tag="IW833_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307749.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(16758..17426)
                     /locus_tag="IW833_001627"
     CDS             complement(16758..17426)
                     /locus_tag="IW833_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1307750.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            17560..17847
                     /locus_tag="IW833_001628"
     CDS             17560..17847
                     /locus_tag="IW833_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307751.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            18263..19801
                     /locus_tag="IW833_001629"
     CDS             18263..19801
                     /locus_tag="IW833_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ1307752.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            19798..20763
                     /locus_tag="IW833_001630"
     CDS             19798..20763
                     /locus_tag="IW833_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1307753.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            20750..22072
                     /locus_tag="IW833_001631"
     CDS             20750..22072
                     /locus_tag="IW833_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1307754.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      22135..22239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22313..23056
                     /locus_tag="IW833_001632"
     CDS             22313..23056
                     /locus_tag="IW833_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1307755.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            23071..24063
                     /locus_tag="IW833_001633"
     CDS             23071..24063
                     /locus_tag="IW833_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1307756.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            24075..24812
                     /locus_tag="IW833_001634"
     CDS             24075..24812
                     /locus_tag="IW833_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1307757.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            24802..25524
                     /locus_tag="IW833_001635"
     CDS             24802..25524
                     /locus_tag="IW833_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1307758.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(25608..>26027)
                     /locus_tag="IW833_001636"
     CDS             complement(25608..>26027)
                     /locus_tag="IW833_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ1307759.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         8954 a         4426 c         5428 g         7219 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgactttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtca cctgatggaa aatcgacaag
     3481 aagtacaaga attaacttta gaagaagtga tgggtgaccg cttcggacgt tattccaaat
     3541 atattatcca agaacgtgca ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc
     3601 ggatattatt tgccatgaac aaagatggca atacgtatga caaaggattc cgtaaatcag
     3661 caaaatctgt cggaaatatc atggggaatt atcatcctca tggtgaccgc agtatttatg
     3721 aagcgatggt ccgcatgagc caagactgga agttgcggga agtactgatc gaaatgcacg
     3781 ggaataacgg gagtatggac ggtgatccac ctgctgcgat gcgttatact gaagccagat
     3841 tatccaagtt aagtggagag atgctagcag atatcgagaa agaaacagtc gatctcgtgt
     3901 ggaattttga tgatacagaa aaagaaccaa cagtccttcc agctcgatac ccaaaccttc
     3961 tcgtcaatgg atcgacaggt atatctgctg gatatgcaac agaaatcccg actcataact
     4021 tagctgaagt gatcgatggc acaatctata tgatcgatca tccacaagcg agccttgaaa
     4081 aattgatgga atatattcct ggacctgact ttccaacagg agggatcttg caaggaaaag
     4141 atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa
     4201 agatagaggc aatgaagggt aataaacagc aaatcgtgat cacagaaatc ccatatgaag
     4261 taaataaagc gaccttggtg aaaaaaatgg atgagatccg tttgaataag aaaatcgatg
     4321 gaatagctga agtacgagat gaaagtgata gaacaggtct gcaaattgtc gtcgaattga
     4381 aaaaagacgc caatgctcaa ggaattttga attatttgtt caaaaacact gagctacaga
     4441 tcaattacaa tttcaatatg gtcgctatcg accatatgac tccgcatcaa gttggactga
     4501 aagacatttt aagaagttat atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat
     4561 tcgatttagc caaagctcaa aaaagacagc atatcgtaga aggattgatg aaagctttat
     4621 cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa
     4681 agaatttagt cgacgtcttt caatttacag aagaacaagc agaagcaatt gtcacattgc
     4741 aattataccg tttgacgaac acggatatca cagagcttca aaaggaatcc gaaagcttga
     4801 ttgcgcaaat cacagagttg aataaaatcc tttcaaatga caaagagctt ttctctgtta
     4861 tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg
     4921 aagacgaaat cgaagaaatc aaaattgata cgcaagtctt agtggcacaa gaagacgtaa
     4981 ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag ccttcgttct tatagtgctt
     5041 ccaaaccaga agaaatcggg atgcgagaag gcgactactt gctatactca ggtgaattga
     5101 gtacattgga ccatgttctt ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc
     5161 atgaactacc cgatttgaaa tggaaagatg ctggtgagca catttctcaa acaatctcca
     5221 atctgtctgt agacgaatcg attttagctg ttttcccata tcaaaaaatt caagcagaga
     5281 aaacatttgt attcatcagc aagaatggat tgatcaagca aacacgaatg acagagttcg
     5341 aaccatggcg cacctataag agccgtccgc tcagctgtat gaagctaaag gcggaagaag
     5401 atgaactgat tgctgtgtat ctgaaagaag atcagccagg cttagatgtt ttcttagtaa
     5461 cgcatcaagg aatgggtttg cgctatcctt taacagatgt tcccgttgtt ggagcaaagg
     5521 cagctggagt gaagtctatg aatttgaaag atggagatag gattatcaac gggctgcttg
     5581 tgttagcaga aggtgatacg ccgatcgtaa tcgttactca gcgaggagct gttaaacgca
     5641 tgcttgctca agaaatcagc caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg
     5701 agctgaaaaa aaatccgcac cgtattatct atatgtcgga aggaagatca aaagaaataa
     5761 ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc aactgatttc cctataggtg
     5821 atcgaacatc gaatggatca tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag
     5881 ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga
     5941 cgccatctgt attaatacag gtggcgtctc tttttcattc atttttaata aatccttata
     6001 cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat gttggtatgt caacgttgat
     6061 ttaaatatat gtaaataaaa cataaaaatt gttaaataaa aaacaagaaa acccttgcaa
     6121 agatagggtg ctgtgttata ctaatcacgt aaaaactgtt acataaaaca gaaatttagg
     6181 aggtccatac aatggagcaa tggaatggat ttaaaggtac caaatggaaa gaaggcgtag
     6241 acactagaga ctttattcaa aataactaca ctgaatatcg cggtgatgac agctttttgg
     6301 aaggcccaac agaagcaaca gaaaaattat gggatgaatt gcaaaagtta aatgatatcc
     6361 aattcgagcg taacggtgtc tatgatatgg acacagaaat cgtatcaact attacttcac
     6421 acgatgcagg ctatttggat aaagatttgg aaaaaatcgt cggtctacag acagacaaac
     6481 ctttgaaaca agcattcatg ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa
     6541 gcaatggcta tacaccaaac gatgaattga cacatatttt cactgactgg cgcaaaacac
     6601 ataatcaagg tgtatttgac gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa
     6661 tcattacagg gcttccagac gcttacggtc gcggacgtat catcggtgac tatcgtcgtg
     6721 tggcattgta tggaatcgac tatttgatgg aacaaaagaa aaaagatcat gatatgactg
     6781 gttatcatac aatgagtgaa gaagtgattc gtttacgtga agaaatcact gaacaatatc
     6841 gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg ctatgatatt tctaaaccag
     6901 ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg atatttagca gcaatcaaat
     6961 cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc attccttgat atctatatcc
     7021 aacgtgattt ggataatgga gtaatcacag aaaaagaagc acaagaattg atcgaccact
     7081 tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc tgaatataac caattattct
     7141 caggattccc tatctgggct actttatcca ttgcaggtat gggattagat ggtcgttcat
     7201 tagttacaaa gaatgatttc cgtctgcttc atacattaac aaatatggga ccttcaccag
     7261 aaccaaactt gactgttttg tattctgaac accttccaga aggattcaga acatatgcta
     7321 gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa tgataatcag cttcgtgaac
     7381 aatggggttc tgatgattgt gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag
     7441 acatgcaatt ctttggcgct cgtgctaact tggctaaaac actattatat gccatcaatg
     7501 gcggagtcga tgaagtgact ggcgcccaag ttggaccaga atatcgtcca atcacatcag
     7561 aaatactaga ctttgaagaa gtgaaacata acttcatgaa tatgatggat tggttagcag
     7621 aactatatgt caatacgttg aacatcattc attatatgca tgataaatat tcttatgaag
     7681 cagctcagct ggctttgatg gattctgact tgcaacgcac atttgctaca ggtattgctg
     7741 ggatctctca tgcggcagac agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc
     7801 gtaatgaaaa aggaatagca gttgattttg aagtggatgg cgacttccct aaatacggaa
     7861 atgatgatga ccgtgcagat gaattagttt catggatctt ggaatacttc atgggacaaa
     7921 tcaaacgtca taaaacttac cgcaactcaa aaccaacaac ttcattattg acgattactt
     7981 caaatgttgt ttacggtaaa aatacaggaa atactcctga tggccgtgaa gctggaaaac
     8041 cacttgcgcc aggagcaaat ccaagttaca atgcggaaca aagtgggtta ttagctagcc
     8101 tgaactcaac agctaaaatg aattacggct gtgcattgga tggaatttca aatacccaaa
     8161 caatcaatcc ttcaggtctt ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg
     8221 tcttagatgg atacttctca aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg
     8281 atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc gaacttgacg atccgggtat
     8341 ctggttatgc agttaaattc cgtgacttga caagagaaca acaattagat gtcttgatga
     8401 gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa
     8461 aggacgttga atagccatcg catcaaaatc gaataatcag tatgcttatt tgaaagaaac
     8521 gatttacagg ggcaggactt gtatgttccg tccctgtaaa ttttacacat aacgaagcgg
     8581 caattgcgaa aaattttgtt cagaaagctt cgcttcgaca gctaacttgt acttaaaatg
     8641 aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac aatcggctat gttcattcta
     8701 tcgaaacttt cggttcagta gacggacctg gactgcgctt tgtcgtgttt atgcaaggat
     8761 gccgcatgcg ttgccaattt tgtcacaatc cagatacttg gaatattggc ggagggaaag
     8821 aatacacagc agatgaactt ttagataaag cagaacgctt cagaccttac tggggagata
     8881 aaggtggtat tactgttagt ggtggagaac ctctattgca gatcgatttt ttgatcgaac
     8941 tatttaaaaa agcaaaagag cggaagatgc atactacatt ggatacatgc ggaaaacctt
     9001 ttacgtatga agaacctttc ttttcacgtt ttcaagaatt gatgaagtat acagatttac
     9061 tgttatttga catcaaacac atcgataatg aagagcataa aaaactaacg cattgggaca
     9121 atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat caacaaaccg gtttggattc
     9181 gccatgtact agttcctgag cgaagtgatt atgatgaata tttgatccga ctagacaact
     9241 ttattcaaac cttgtcaaat gtagacagag tagagattct tccttatcat acgatgggaa
     9301 aatacaagtg ggaaacactt ggtttgaaat atccattaga agggatcgaa ccacctacaa
     9361 aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga agagtataca aaatatttaa
     9421 cgcgataggt cgcatcggaa agaagataag tattgcttct ttccgttttt ttgctattat
     9481 taaataggag aaatcacaaa ggagcgaatg acatgtcaaa aattttagtt tttggtcacc
     9541 aaaatccaga tacagatgca atcggggcag ccattgcgtt ttcacacttg caaaatgaat
     9601 taggtaaaga aacagaagca gttgctctag gtacacctaa tgaagaaaca caatacgcat
     9661 tagaccactt tcagctatct gcaccacgag tagtagagtc agctaaagca gaaaatgcag
     9721 atgtcatgct tgtggatcat aatgaattcc aacaaagtat ttcagatatc agcgaagtaa
     9781 atattttagc tgtagtagac caccatcgta ttgcaaactt tgaaacagct aatccattgt
     9841 actatcgtgc agaaccagtt ggatgtacaa gtacgattat tttaaaacta tataaagaaa
     9901 accaagtaac aattcctaaa gaaatcgcag gaatcatgct ctcagctatc atttcagata
     9961 cattgttgtt caaatcacca acatgtacgc ctgaagatga agcagctgca aaagaacttg
    10021 cagcaatcgc tgaagtcgat ctaaacactt atggacttga aatgttgaaa gcaggaacaa
    10081 atctaagtga taaatcagct gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg
    10141 gcagttccaa cgttcgtatc gctcaagtca atacagtgga ccttaatgaa gtgatggatc
    10201 gacaagcaga gctagaagca gcaatggaag atgaaatgaa agcaaatggc tacgatctgt
    10261 tcgtattagt tgtcacaaac atcttagaca gtgattcaga attacttgtt gctggtaaag
    10321 gaaaagaaaa agtagaagaa gctttccaaa caactttgac caacaaccgt gctttgctta
    10381 aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt gacggctgta tttagctaat
    10441 caaatggtag tatgaagcat tcaaaatgaa tggataagcg attgatataa aaagaaacat
    10501 agaacatact aaaggccgaa gaaatggagg agaataacag tcaattatga ctcttgttct
    10561 tcctcctttt tacgtaaaag ccttttaagt aaaataaaac ggatagaaaa gagaacatat
    10621 atgaactatt attcaaaaaa taaggaaaac ttttatcaaa aactgaccga tgatccgctt
    10681 ttttcttcat tgaccgatta tctatatgaa catcgagaac aagaaacgat tctaagagaa
    10741 ttgaaaaaag aattttcgca aaataaattt tcccattttc ttgatttatt gatcgatgca
    10801 ggattaatca agagggaaga acgaagatat catttgaact ttccaatatt tgatcctaaa
    10861 gattacttgc aacaagccac ttcggctgct gaaaccatag ctgaacaatt gaagagactc
    10921 tctgttgatg aacaaaaatt agcgatggga gaagttattt gggcgtattg tttcgaagat
    10981 gaacggaaag aagcgtattt ttatggagta agaaacagcc gagagacaga attgctgagg
    11041 acaactgcag gaaatgagaa gtaccgtttt atcacacttt cctctataga acacttcccg
    11101 cttactttgg ctaattactt ttttgtacag aagaatcagc ttcctgtaac gaaggcattc
    11161 aaagaattag ccgaacttat tggagacgta aatgaatcgt acttttttga tcaaatagaa
    11221 gttatcgttg acaggatacg taagaataaa tataaaaatc gccggcctag tattttccat
    11281 cagtctctac ttgtgacgaa tactataaag gaagaagaat cattcacatt agaattacct
    11341 attgtcgaaa aaaacaattt tgagattgaa ttaccaacat tggatccttc attgacaatg
    11401 gaggaaatgg cctttcttaa acgacaaatc ttttctgagc tgagcaagaa atttataccg
    11461 catgcatttt cttatatcaa agaatatgga acagttttag tgagtaaaac ctaagagagc
    11521 agtaagcaaa aattatactg actcttgcag gaggagcctt tctcttcctt tatggttaaa
    11581 agaattattt gagtattttg cctatctacc acctattaga ttcactacat ggtctggaaa
    11641 caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca aaggaagtag tgataagaaa
    11701 agttagattt ttaaataatc aatattatga ttcagtcaag tatggcattt tatgggaaga
    11761 actagctgat tagattagga agtccatttg ataatccaga atcactaaga aacgaatggc
    11821 gagtcattaa taatttatcc aatcttgggt tattctttta gattgaagtg tttaaaacta
    11881 aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat gctttttatt ttgtttgttt
    11941 tttctttgcc acataaatac acctagactg acgatgaaga gcatatccaa cagatttcct
    12001 gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag caaggacaaa aaaggaaaac
    12061 aagctgtaaa taaaacaggt gacaggaact aatcgaaaga agagcggaca tttcaattgg
    12121 ctaaaaatga gtgaaaaaat aaaataagta aaaatggata aaatcacgac taaaccattc
    12181 atgataaaga agaaaagcgt attgtgatta aacgaataaa aaagaattgc taaaaaagaa
    12241 aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta acgtaccagg atgtcgatga
    12301 aaaaaatagg caccgaaata aacagaaaac agtaagccgc aaagagaaga caaaatcaaa
    12361 aaaaacggat tttcttcggc ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat
    12421 gcactagaag gcgcatgtga ggtcttaagg tatgaataac atagaaaaaa taagatcgtc
    12481 aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg ttctacgttg aatttccata
    12541 atatctccaa attaaattga taagtatata aagtataata acaaattgat gagtatatgt
    12601 cgatgttttt taatcaaaat aataaataaa aactgtctaa catttaaaat ttaatttaga
    12661 tattagacag tttttaagtt tgtgagttat tttccttgtc gttttctcac ttttttagtc
    12721 cagaaaccac ctgaaatata ttttggctca taagaaatga taaaagcacg ctcttcgatt
    12781 tctttgatca ggcgatacag ttcacgttcg ctttttctag gagaaagtat ttcgagaact
    12841 agccgctcac cttccagccc cattgcagaa gtctgtgtca caccataacc gtgttctctc
    12901 agtatacccg gcaaactttt atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg
    12961 atgtatccta atgccaattt gtcttcaatc aaaattccga cactgatacc tactgcgtaa
    13021 cctaacgcat agacaaataa attcagtgga ttatctaaac gatctaaaac aagggataag
    13081 ccgacgatat aaatcgtgat ctcgaccata ctgacaagtg gagcaatcag tcgatagcct
    13141 ttcattgtca acatgaaacg taacgtgttc agagtgatat aagcaaaatt aatgaggaag
    13201 atcatcccca acatttttgg atcaatcatt tgtaaaactc ctttcattta tataacaaca
    13261 atatcaaaaa aaatcgtaag tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata
    13321 aaatgattat aatagaatta taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg
    13381 tgtgttttgt gccagccacg tgctaagtct ccttctcagt atatacttag gtgtctattc
    13441 gattcaacag ccaattgagg tagagaacat tgtttttcag atgtcgctga cagatggttt
    13501 aattttaagc gtttttttct ttttgatact gtttattggt aatattttcg gtagcgttgc
    13561 cagttttttg aatttttcta tttatccact cctttcgcta gcattaggag tggtgggact
    13621 ttgttctctg tgcttatttc ccgagccatt cagtcctgca tttatattat ttggtacact
    13681 gaacctgttc cagacaactg ttggagcatg gttgctatgg cgatcaggaa atttaatgaa
    13741 aataggtgag taacaattga atccaattat attgaaacgt tctgtccatc atattccagt
    13801 gttagaagtt gttctgaaag aattaagaga ggaacgtatt cccgttgtca tctattatca
    13861 cggatggcag acaagtaagg agttagtttt gacgcaagga agaaaaattg cgcagcaggg
    13921 cattcgtgtg atcctgccag atgcgatgaa tcatggggag cgcaaacagc cggtttcttc
    13981 tattccatcc tatacatttt gggatagtat ctacggaaat ctgtttgaat ttgacacact
    14041 tatcaagcat ttagaaaaaa gaggtcttct acatgatcag cttgcagttg gcggggtttc
    14101 gatgggggga atgacaacat gcgccttgtt ggctaaacat cctcatatag tagcgggcgt
    14161 gtgtttgatg ggttcccctg cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc
    14221 ggaatataat tatgtcgtac caaaagacta ttttgatttg atttcatggg ttaagagcta
    14281 tgatttgtca ttgcagccgg aaaagttagc tggacgtcct ctgttctttt ggcatggaca
    14341 agctgacgaa aaaatccctt atatacaaac ggaacgattt gtagaaaaaa atcggcataa
    14401 gtcttacgga aaagaaattg tttttcgttc ctcaaaaaca cagcgacata tggtacaaat
    14461 accactaatg gaagaagctt ccactttttt agcaaaaaat ttgataaaaa agtataaata
    14521 attgaaacgg aaaatcactc atgacgatag aggattttcc gttttttatt ttgggctata
    14581 ataaactctg ttaggaaaat ttggtataat tgatagtaag aatgacaaca tgttagaaag
    14641 gaagagagca tgcccacttt agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg
    14701 gtctctcgag tcatcaatca tccggaacag gtgactgacg aattgaaaga gttagttttc
    14761 tcagcaatgg ctgaactgga ttaccgtcca aatattgctg caaaagcact tgtcacgaat
    14821 cggtcgcaga tcatcaaatt atttatactg gaagaaatcg acacaactga gccctattac
    14881 atgaatttgc tgatgggaat cgctaaacgg atcggtaaag cgcattattc tctccaatta
    14941 gttacaaatg atgccttcga cgtcggttct tgtgatggat acatcatcac aggtgtcaga
    15001 aaaaaagact tcgattggat cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat
    15061 tctgaaggat atgattatgt agacagcgat aatgaatatg gtacagcaaa agcaacgcaa
    15121 tatgccttag aaagagatta tcaaacgatt atttatatcg gtatcgaagt ggacgagcct
    15181 tttgaaattt caagggaaac tggttattta tctgtgatga aggaagttga tcttcctagt
    15241 aaaatcttgc ggtttccaaa tcattcgaca gaagtagaaa gatatatgac agaaaactgg
    15301 gcagatttta cagataaaac ttgttttgta tgtagttcag atcggctagc actaggagca
    15361 gtaagaggga ttataaatgc tggaggacac ttgcccgaag aatttggtgt gattggattt
    15421 gatggggtat ttttagacca aatcagttca cctaaattga caacgatcaa gcaagatatt
    15481 atacgtatgg gtgaagtatg tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg
    15541 agtcaaggtt atcgtcgttt ttttcctgaa ctagttatca gagagacaac gaattaagtc
    15601 ttattactat atatatattt atgaaattca tcgctatttt cttaaaataa tcagcgcatt
    15661 gctactgaaa acaatgaatc tgtgctatta ttgagaaaag ttccattttt gagaaggatg
    15721 gtttgaatga aagaaatcag acgaaaacat ttccgttttc ctgcgtacga tgatgagtta
    15781 ggcgtaaaat taaccaacga caagaagcga gtattatttg acggacaaga agatcttttg
    15841 actgaacaga atcagtcccc gtttgaacag atgaaagaat ttcatttcac gaacacgaac
    15901 accgagaaaa tccgtcgcaa gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag
    15961 gaaaatttgg aaaagcataa aggaaaatta ccggattata ccggccatta ccaatcagaa
    16021 atcacatcga caggacgaaa aaaacttttt ggtgattcct ctcgttctgt cagacaaagt
    16081 gaaacaagga cagagtccaa gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg
    16141 gagcgttctt attttgttcc taaatatgta cccgcatcga ttatcccaga ccctagagaa
    16201 agcaaaattt ctgaggatca tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg
    16261 ttgttcgata cagaaccgac gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga
    16321 acattcaatc gaacaaaaac gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa
    16381 aaaagccatg gtatcttaga acgctcatta caaggattga tcgatgatca aggaaactct
    16441 ttgggagaaa atagctattt taaataagtg atatacaaat ttatattcgg ctacagttgg
    16501 aaaggaaacg ctgcggcgtt tccttatttt atactctatc cgaaagttta tatcgtatcc
    16561 tcaaattttt ttgagtttaa gcgaattttt tctcccaata agtaagaaaa taatggcaaa
    16621 ataaaaaaac aaactatttt cgcagatcgt ttgcaaaaat agtttatttt tttataaaga
    16681 agattttact gaataatatc attttagttt acataatgat atttctgtaa attaaataaa
    16741 gtagtgaaaa ataagaatca gtgctctaac tgttcaagag gatcaagtgg ttcaaatgtt
    16801 tctgtagtct gttttgcttc caattggggc aaaacagatc caatttccag atacttcact
    16861 ttattcaatg tataagatag aatagaacag ctaagtaatg tatacagaaa atgtgtgaga
    16921 tccaaataaa gcaaggagac aactaaaata ccaaaagtaa tcaagaaaag acttttgcca
    16981 atcttaatat gaaaatattt ttcaatggct tgataaatga tatctgttcc gcctgtgctt
    17041 ccgccgaatc gaaagacgag accgcaacca atgccagaaa gaatacctgt catcaaacta
    17101 ttgatcagta gactatctac agcaaaatgt atatgaaggc tctcccataa atgtaaaaag
    17161 aaaactagcg caaatgttcc gacgatcgtc cggagaaaag cgtgttttcc aaaaagaaac
    17221 aatgagataa gcaataaagg taaattaaaa agcagtgaag tgacagaagg agcgaatcca
    17281 aaaagattat gcaaaagcaa agcaatacca gtaaccccac catctgccag cttagatgga
    17341 atattgattc ctgtcacagc taatccgtag attcctgcgc ctaaaatgat tccgcacaat
    17401 tctgtaaata aatttaattt tttcatttct agcctctttt cttttgaaaa ttttcagcaa
    17461 cttctttatt atactgactt atactcttac ttcaagagga aaatgaaaaa aaattatttt
    17521 tggctgtttc tttttattta tcaaataagg agtggaaaaa tgcgagcagc agacttattg
    17581 acaattctta caaaatcacc caaaaaaaca gagtatcaag gagttttttt ggctcaagaa
    17641 caaaaactgg tgcctgtgac aatggtccag ataaataaag caaatcaatt tatcttttta
    17701 tttgaacacg gcaaagcacc acttgctatg aaagacatct ttcttttttt gatgaagaac
    17761 cgtgagaaag ctgtttatta tcagtcaaaa gaagaactca ttcctcttta tggagtgaaa
    17821 gagatagaaa ataaagtggt gatataagta aaaataagaa aacaagacgt ttatgatgtc
    17881 aaaacaacca ttttttacat ttttggcaaa aaagtgtcat gatacagtat aatattcgtt
    17941 gaacaggcca ataaaggtgt tctcccccac gagatttcta ctttattggc tgtttctttt
    18001 tattaaaata aaagttgtgt gtgtaaaaaa gatatagctg attccaagtg acaatttcgt
    18061 atagattttt atggtattcg tagtgaaaaa acgagaacca aaaagttgat ccgactttgt
    18121 tgcttggatt ctttttttgt cagcatattt agaggtattt ttttcttttt tttgttcgtg
    18181 ctgtaaggtt aaggcaagac aagttagttg ggaaaaagga aagaaatgtt tatacttaaa
    18241 gaataacaga aaggaaggtc gcatgaataa ttttatacaa acattaagtg aaagaaaagg
    18301 cgagttgctg gaagctgcat ttcaacatct gtccatttct ctcatttctt tgctgattgc
    18361 agcactgatt gctattcctt tagcgatttg ggcagccaat cataaaaaaa tggcagaagt
    18421 cttattgcag atcacgggtg tgctccaaac gattccttca cttgccttat taggtttact
    18481 tatcccattt gtagggatcg gaactgttcc agcattgatt gcacttgtta tttacgcttt
    18541 gcttccgatt ttccaaaaca cgtatattgg tctagctgaa attgacccgt ctattgaaga
    18601 agcagcggta gcatttggta tgtccagaat gagacgcttg ctgaaagtag agcttcccat
    18661 cgcattacct gtcattatct caggtatccg aactgcctta gttttaatca tcggtacggc
    18721 tactttggca gcattgatcg gagcaggtgg attagggaca tttattttgt taggtattga
    18781 ccgaaataca cctgttttga cattgattgg tgcgatcagt tctgccttgc ttgcaattat
    18841 attcagcgga ctgattcgct ggttgcagca taagaaacct cgatattcca tcattacttt
    18901 agcagcagtg gtgtgtttat ttggcggcgt ttctttatat cagtctgcct tgtttaaaaa
    18961 tgaaaccatc acgattgctg gaaaattagg agcggaacct gaaatcctga tccaaatgta
    19021 taaagaattg attgaagaca acacaaaaac agaagttgaa ttaaaaccga attttggcaa
    19081 gacgagtttt ttgttcaatg cactggatca aggtcaaatc gatatctatc ctgagttcac
    19141 aggtactgtc ctagaaagct tggtgaaggt acctgaagca atgaaaagtg ctgatttgac
    19201 aaaagaaaaa acatatgacg aagcagaaga attattgaat aagcaattcg atatgacatt
    19261 attaaaacca atggcttacc aaaatacgta tgccttggca gtcaagagat cttttgcgaa
    19321 cgaacatcaa cttaaaaaga tttccgattt ggcagctatc tctgatcaga tcaaagcggg
    19381 attcacactg gaatttattg atcgttcaga tggatataaa ggaatccaag aagcatatgg
    19441 cttgaatttt tcaagtgtac aaagcatgga gccaagtttg cgttaccaag caatcaataa
    19501 tggagatgtc aatattgtgg atgcctattc cacagatagc cagctcaagg agtacgatct
    19561 agtgactcta gaagatgatc gagggttatt cccagcttat caaggcgcac cgctgatgaa
    19621 aaaagatttt gctaaagaac atcctgaaat cgttaaagca ctgaataaat taagcggaaa
    19681 aataacagag gatcaaatga ttgatatgaa ttaccaagtc aatgtcgaaa agaaaaagcc
    19741 gacagaagta gcacgaatct tcctgaaaaa tgaaggatta gtaaaggagg aagatcagtg
    19801 actaaattaa tcgagttcaa acatgtgcaa aaaaagtatg atggaaagta tgtcatcgat
    19861 gacctaaatc tggcaatcaa aaaaggagaa atctttgttt tggttggtcc ttccggtagt
    19921 ggaaaaacaa ctactttgaa aatgatcaat ggattatcta aaccatcagc aggagacatc
    19981 tacttcaaag gaaaatcgtt aaatgaatat aatctgcaga aaatgcggtg gaatatggga
    20041 tatgtgctgc agcaaatcgc tttgttcccg accatgacag tcaagcaaaa tattgaagtc
    20101 atccctgaaa tgttaggttg ggaaaagcag aaaagagcag accgtgtaga tgaacttctt
    20161 caaaaagttg gcttatctcc tgatatttat cgagatcgta tgcctagaga gttgtctgga
    20221 ggagaacagc aacggatcgg gatcatccga gctattgctg caagcccaga tgtgattttg
    20281 atggacgagc catttagcgc attagatccg atttcaagaa actcattaca agaattggtt
    20341 ttgtccttac acgaagaatt gggcacgacc atcgttttcg ttacgcacaa tatggaagaa
    20401 gcaatcaagc taggtgatcg tattgcattt atgaaggatg gagaaattat ccaatgtgac
    20461 acaccagagc agttattgat gaatcctaaa aacgactacg ttcggcattt ctttgatgaa
    20521 ccgaaacaga cgaaagagtg gcgagtagaa gatttagtcg tcaatggata tttcttaaat
    20581 gaaataccag aaaatgctcg tcaacaagtt tgctttgata cgcctatgaa agaagtatat
    20641 tcgcttcttt cggtgtatcc aagcattgtg atagtagaaa agcaaagaag tattgggtcg
    20701 ctgacaagta aagaaatctt tgctttttta agtcgagagg aggaaggaaa tgaaaacata
    20761 tgatgcaatc atcatcggtt caggtgtgag cggcttaagt gctgcttatg gattgaaaga
    20821 agcaggtaaa acagtactag tggtcgaaga agatctatgg ggcgggactt gtcctaaccg
    20881 tggatgtgat cctaaaaaag tcttgttaag tgcagtagag gcacgtaatc gggtaaaaca
    20941 attgagtgga aaaggtttta acgagatccc aactgccaac tgggaagaac ttcaaaaatt
    21001 caaacgaaca tttacggatc cagtcccaga aagccgaaaa aaacaattag cagaagctga
    21061 aatcgatcat ctttcaggaa ccgctcgttt tttggatgat tcatctattg aagtcaacga
    21121 agaagtcttc catgcggatt acttagtttt ggcaaccggg caaagaccta cgatcttacc
    21181 agtggaggga aaggaatact tgaaaactag tgcagacttt ttatcattgc ctgtactgcc
    21241 taaagaaatc atttttatcg gtggtggtta tattgctttt gagctagcta cgattgccaa
    21301 tgctgcaggg agcaaagtaa ccattgtcca tcataatcaa cggccattaa aagaatttga
    21361 ggcgtctttg gtagaggaag ccgtccatca aatggaagca tctggcattc agtttgcttt
    21421 tggtgttgaa acccaaaaga tcatttcaga aggtactcgc tatcgtttag tcggaaaaga
    21481 gacggaatta gtagcagata tgattttttg tgcgactggg agacagccga atacagaatc
    21541 tttagcactt gagcaagcga atatcgtctt tgataaacac ggaattgcag tcaatgatta
    21601 tctgcaaacg agcaatccaa aaatttttgc gtgtggagat attgtttcaa ggaaaacccc
    21661 aaaattgaca ccagtcgcca cttttgaagg aaattatgta gctaagcgga taacggatgc
    21721 aacaagcgaa cctatcaaat atccgattat acctacgatc gtctacgcca gtcctaaact
    21781 tgctgaagta ggagttacaa aaagtcacgc ctcttcgtca gatcaagtgg tagagatgga
    21841 tcttaccagt tggttcacct atcatcgtgt caatgaacca gttgctaagg ctgaattgac
    21901 ctttgaccag caaaattatt tgattggtgc tgcagtgatt agcgagcaag cagacgaatt
    21961 gatcgatgat ttgacactcg tcatcaatca aaaattgact aaaaaagagt tggatagcta
    22021 tatcatgggg tatccgacat tggcaagtga tctctcctat ttattgaaat aatcagcatg
    22081 ataactaaag cgcttgttgc tttttccttc ttgtgctaca ctttaaaaaa tgaatacctg
    22141 tttctgggga tgtcgtagac gacatgagag taagttgtga acttagaccc attgaacctg
    22201 tcagttaata ctggtgaagg gagaaacata gcagacgctt gaagcgggtt actatgtcgt
    22261 gcccatttca agcgtttttt gttattcata ttagaagaaa ggaggtaaca aaatgaagaa
    22321 aaatctttat ccagcagtca gtatggcatt cattttaatt atttggcaga ttgttgtcat
    22381 agtttggaaa atacctgcct ttattttacc cggacctgct gctgtatttt ctgcattagc
    22441 ggcagatgca caagtattac tttcccattc tgtcacgaca ttaaatgaag cagtgcttgg
    22501 tttggtaata gctagcgttc tttcttttat tactgccttg gcaatggatt attggaagtt
    22561 tttagaaatg tctttttatc cgttactagt gatttcacaa atgttgccaa tcatggtgtt
    22621 agggccatta cttaccctat ggtttggttt cggtatgctt ccaaaagtga tccttgttgt
    22681 cttgataagc tattttccga tcgtagtttc tttttcggat acattgagga aagtatcaaa
    22741 agagcaaatc atctttttga aaacgatggg ggcagataca gggaagattt atcgaatata
    22801 taaaatacca gcgggtttat ccggcttctt ttccggttta aaagtggctg ctacttattg
    22861 tgtcagcgga gcagtcgttg gagaatggct aagcgctcaa gcaggcttag ggtattacat
    22921 gatacgcgta aaaaacagct accagatcga taaagttttt gctgcgatca tttgtgtcat
    22981 tctattgagt ttattgctga atggcgcttg ttcattattg aaaaaagggt attatcgtat
    23041 gctttatatt ttttaagaga aggggatcaa atgaaaaaat tatggctttt attaccgcta
    23101 ctgttgcttt tatccgcttg tggcactgca aaagaaacaa gtagcaaaca agaaataaag
    23161 gatttgaaaa aagtgacact tgttttggat tatgtaccga atactaatca tactggcatc
    23221 tacctagcaa aagaaaaagg ctattataaa gaggctggtt taaatgtcca aatcatcgaa
    23281 ccaggagata attcgacaag tattggtcta gtaggtgcgg acaaagcaca atttggtgta
    23341 agttatcaag aagatgtcac ttatgctcac gcagatggac agaatatacc agtcaaagca
    23401 attgctacag tgatcaaaca taatacttct ggattcgcca ccctatcaga cagtaacatc
    23461 cattcaccaa aagattttga aggaaaaaca tatgcaggtt ggcaaagtcc aagtgaagaa
    23521 gctgtcttaa aagctgtgat ggaaaaagac ggcggggact tttcaaaact gacaatggta
    23581 ggaagcaatg gagaagggcc agaaagctta ggaaaatcct cagatatcca atggtacttt
    23641 gaaggttggg atatgatcaa agcaaaagaa gcgggtattg aactaaacta tatcccattg
    23701 aaagaattag atgaacggtt agattactat actccggtaa ttattacgaa tgatcaactg
    23761 atcaaaagtg atccagaatt ggtacagtct tttatggatg ccacgaaaaa aggctatcag
    23821 gaagctatta aggatccaaa tgacagtgct aaactgttgc aaaaatatgc aaaagaaaat
    23881 gatcggacgt ttttagaaga atcacaagca ttcttgtcaa aaaattatac ggatgatcct
    23941 gagaattggg gattgatgga agaaaaagtt tggcgcaatt atacagcttt tatgcaggaa
    24001 aacggattga tcaagcaaga tgtaccttcg caagaactat tcactaatca gtttataaaa
    24061 taggagtgat agatatgtca atccaaatca gccacttgaa tgtagtcatt gaaaacctga
    24121 aaatattaga agacatccat ttcactatcc ctaaagatac ctttacatcg attgtcgcgc
    24181 caagcggagc cggtaagtca acattgctga aaactttaac aggagtccag cctatgactt
    24241 ctggaacgat caaagtagac gatcaaaaag taactggtct ccatacagca tttagttata
    24301 tgccgcaaga agatatgtta cttccttggc tgagcgtcta tcaaaacgtt acgttatatc
    24361 aaaaaatcaa tcatctaaca atcaatgaag aacaagtaag ggattatctg gatatctttg
    24421 ggttaaacgg ctatgaacat tttttgccgg aacaattatc tggcgggatg aagcagcgaa
    24481 cggccttgtt acgaacaatc atgaatcctt cttcttactt gcttcttgat gaaccatttg
    24541 gagcgttaga tgctatgacc agagggcaga tgcaagactg gatgttgaag ctgcctaaaa
    24601 aagcaaagcg gactacttta cttgtgacgc atgatatcga agaagcaatt tatttatcag
    24661 acaggattct tgtgctttcc gcccgtccgg cacatgtgat tgctgagatt caagtgcctg
    24721 aaaaacgacg gtcacgtgaa tggctactgc agcaatcaga gttgaagcaa acgatttatc
    24781 agttattggc aggtgaatta gatgttaagt gatgctcatt gtcatttaga tgggagtcag
    24841 agtttagcac tccttcagca ggaacacacg attttgacga tcatcaattg tgattcgcca
    24901 gaagaatgga aggaaaacag acagcttgca gcaagtaaga ctcaagcctt aagttacggt
    24961 atccatcctt ggaaagcaga tagctatacg tttgaacaag ttgagccttt tttgaaaaaa
    25021 gcaaggatca tcggtgaaat agggttagat aatatttgga cgaatgtacc gatgactaca
    25081 caaaaaaaag tatttgagcg acagcttgct tttgcagcca taaatgagaa acctgttgta
    25141 ctgcacacaa aaggatgcga aaaagagatc ttaaattata tccaagctta ccctaatcac
    25201 tatttgatcc attggtattc ttctttggac tatcaacaag actatattga tttaggctgt
    25261 tatttcagca ttggtcttga tttgaaaaag aatccagcag tctggcaact agctaaagca
    25321 gtcccaatcg accacttact tattgaaacg gatggactac atgcagcaga gtgggcttta
    25381 ggggggattt tttcagaaaa agattatgtc tcactgcttg aagaacagtt aaagacaatc
    25441 gctgagatca aagagctatc agttgaagaa gttaaaaaaa atacttatca aaacttggaa
    25501 cgtttcatta gattaggtaa ataaaaaaga gatgtgacac aactagtgtc gcacctcttt
    25561 ttggctttct ttgtataaaa gtaaactgtt tgattaataa atgaatctta gtaccaaccg
    25621 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    25681 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    25741 tggtaacgtc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    25801 gcgatccatt cttttgcaga gcttgtgctt gctggagtag cagcagtttc tgttacagca
    25861 ggagcaggtt gtgtttcagc aacaggcgct gcttgttttt ccgtttgaac cgtttcttgt
    25921 acaacaggtt gttcagcttg gactggtgct tcttgaacgg ctgcttgagg ttcagcagct
    25981 gcttgtgcag agccttctgt tgggattgtt aattgttgtc ctgagta
//
LOCUS       DACEQH010000036        25944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.036, whole genome shotgun sequence.
ACCESSION   DACEQH010000036 DACEQH010000000
VERSION     DACEQH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.036"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(85..2352)
                     /gene="gshAB"
                     /locus_tag="IW833_001637"
     CDS             complement(85..2352)
                     /gene="gshAB"
                     /locus_tag="IW833_001637"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ1307760.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(2539..3537)
                     /gene="ftsY"
                     /locus_tag="IW833_001638"
     CDS             complement(2539..3537)
                     /gene="ftsY"
                     /locus_tag="IW833_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ1307761.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(3549..4358)
                     /locus_tag="IW833_001639"
     CDS             complement(3549..4358)
                     /locus_tag="IW833_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1307762.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMVHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(4367..7948)
                     /gene="smc"
                     /locus_tag="IW833_001640"
     CDS             complement(4367..7948)
                     /gene="smc"
                     /locus_tag="IW833_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ1307763.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(7968..8654)
                     /locus_tag="IW833_001641"
     CDS             complement(7968..8654)
                     /locus_tag="IW833_001641"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1307764.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(9002..10783)
                     /locus_tag="IW833_001642"
     CDS             complement(9002..10783)
                     /locus_tag="IW833_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1307765.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(10809..11723)
                     /locus_tag="IW833_001643"
     CDS             complement(10809..11723)
                     /locus_tag="IW833_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1307766.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(11750..12712)
                     /locus_tag="IW833_001644"
     CDS             complement(11750..12712)
                     /locus_tag="IW833_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1307767.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(12712..13656)
                     /locus_tag="IW833_001645"
     CDS             complement(12712..13656)
                     /locus_tag="IW833_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1307768.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(13657..14661)
                     /locus_tag="IW833_001646"
     CDS             complement(13657..14661)
                     /locus_tag="IW833_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1307769.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(14929..15171)
                     /gene="acpP"
                     /locus_tag="IW833_001647"
     CDS             complement(14929..15171)
                     /gene="acpP"
                     /locus_tag="IW833_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1307770.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(15293..16294)
                     /gene="plsX"
                     /locus_tag="IW833_001648"
     CDS             complement(15293..16294)
                     /gene="plsX"
                     /locus_tag="IW833_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ1307771.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(16343..18379)
                     /gene="recG"
                     /locus_tag="IW833_001649"
     CDS             complement(16343..18379)
                     /gene="recG"
                     /locus_tag="IW833_001649"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ1307772.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(18496..20178)
                     /locus_tag="IW833_001650"
     CDS             complement(18496..20178)
                     /locus_tag="IW833_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ1307773.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(20230..20592)
                     /locus_tag="IW833_001651"
     CDS             complement(20230..20592)
                     /locus_tag="IW833_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1307774.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            20862..21050
                     /locus_tag="IW833_001652"
     CDS             20862..21050
                     /locus_tag="IW833_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ1307775.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            21288..23957
                     /locus_tag="IW833_001653"
     CDS             21288..23957
                     /locus_tag="IW833_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1307776.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(24048..24668)
                     /locus_tag="IW833_001654"
     CDS             complement(24048..24668)
                     /locus_tag="IW833_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1307777.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            24780..25181
                     /locus_tag="IW833_001655"
     CDS             24780..25181
                     /locus_tag="IW833_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307778.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANS"
     gene            25316..25654
                     /locus_tag="IW833_001656"
     CDS             25316..25654
                     /locus_tag="IW833_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAQ1307779.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         7026 a         5500 c         4644 g         8774 t
ORIGIN      
        1 gaaaaaacac ataaattttt acatgcaaaa aagcttttga tcagattgac atcaaatagg
       61 acaatctgtc aaaagctttt tcttttaaaa tacttcagga tataacaatt tcaatacatt
      121 catcgtcaat cttctgcctt ttcctgcaaa gggataaaca tgcatatgca tggctggatt
      181 gaaatttgct tcgatgatcc catatgcttt tttatcagtt gtaggatcga tttctttatc
      241 tggtataatc aaatcgatac cactaatctt agctcctagt gcttctaccg cagaaacggc
      301 gatttttttg taagcttcag aaaattcatc agtcatatcg atcgaatcac cacctgtact
      361 gatatttgag ttctccctta gataaacaat ttgattggct tggggaactg attcgatcgt
      421 caacccttgt tctttcaaca tcaattgttc taattcacct aattggatca attctaaagg
      481 tgcacgatga ttcgttccac gcaaagggtc actatttttt tcttcaacaa gttctttcac
      541 tgttcggata ccgtcaccaa taacattagc agggactcgc aacatgatgg cttggacttc
      601 tccgtcgatc acaaagaaac gatattccgt tcccggcata aattcttcta ctaaaaccga
      661 actgtcttca cgaaaagcaa tggctaaacc tgcttgataa tcttccagac tagctccttc
      721 cttgaaaatc gtgataccta acccgtaatt ggttgattta ggtttgatca caaatgcctg
      781 ctcggcaaat ctcggataag cttttaccgc ttcttccatg gatgaaaatt ctgcgcctcc
      841 tggtacccgg aacccagctt cttttaagac ttttttcgtt actgttttgt tttccatgat
      901 aagtgggacg atatagctgt ccttacttgt catgtttgca tttttcacat attctacatg
      961 gtcttggaat tgcagacgca aaaattgatc cgactcatct aatactttga cttggacacc
     1021 tttttggatg gcatcgaaca taaatatctg agtagaaagt tccatttcgc gaaaaccggc
     1081 caactgatag ggacgttcat ggctttcttt ataatattga tgtcccaatg ccacagcaac
     1141 ttcttgctga ctcgatagct gtgcttcttg atagagtttc gctgctaacg tttgttcagg
     1201 atgatccatt tgagtcaatg cttcctctac taaataaaga gaatccaacc aatccaattc
     1261 aaccagcatt tgcttcatcc cttcaagaat ttctctacct tcttgatgaa attctgtttg
     1321 atctgatggt tgttctagtg ctactttgtt atttcgagtc gtaccttctg ctacccattg
     1381 gtcacagtct tctttttctt ctgtccataa catgtacatc aggaataatt gtaagaaccg
     1441 aacttgctct ggattgatac ccaatcttgc ataaggattc aaatcgatat tccgcaattc
     1501 aatataacga acacctgttg ttgctaaatc tgcgactttc tttccgcctc ggaaacgaag
     1561 cggtgtataa aattctttct cctccgaaag tttcccttct tcgatcattc gctcgatatc
     1621 tgctaaatac tgtttcatag aagcatagga aactttcaca tttggatgat tcgtatagcc
     1681 cagcgcactg ttacgaatac tgcgaacagg ttcttgcgga tgtgattcat ctaaaaagta
     1741 gttcttctca ctcattggtg aagcaccgaa taaataagtg atcatccagc gatagcgcat
     1801 aaagtttctc gccgttttca aataaagttc tgttttgaat tcggaaaaat cttgaaattc
     1861 ttcctgatgg ctgaacaatg ttcttagcaa ctcatcgcca aattcaaaat tgaaatggat
     1921 accgcttacc atctgttttc gcttcccgta ttcttttgct aaataacgtc gatacaagac
     1981 atcttcgaaa ttttttaatt ttgcaataat gatcgcttcg tctttttccg gtaaggcagg
     2041 tggcatactt aatggccaga tcatctcttc tttcggtata gaacgagcag tcacatcata
     2101 aacagcagcg agatactgaa ataattcggg aatagaatcg gtaacaggag tgatcatctc
     2161 tgtttgtgtt tcgctaaaat ctgtttggat ataggggtga taggatcgat cgccaaagat
     2221 tgctggatga tcggtttttg ctagatttcc tgcaagatca acccgctgtc cttcccgttc
     2281 tataccaaat cgagcttggt cgataaaagg gcgcgcattg acatgcaata ataattgctt
     2341 aaaattcatc attaagtgac ctccaattca tatgaatcta attataatca aaaagtctaa
     2401 gaaaaagaac gaaattgttt gagaaccgct tacaaataag tataaaaatg aaggcatcaa
     2461 aaacacagct atgttaaaaa caatcattca aacaaggagc tgcaactttt gtcacagctc
     2521 ctgcttttta gacatgggtt attcttcttt tagtaatcct ttgaacaatc caacgacaaa
     2581 atcatttgga tcaaacggct ccaagtcatc gattccttca ccaagaccga ccaatttcac
     2641 tggtaaatgc aattcattac ggatagcaag tacgataccg ccctttgctg taccatctaa
     2701 ttttgtcaaa acaagaccgg tcacatctgt tgtttctttg aattgttttg cttgaaccat
     2761 cgcgttttga ccagttgttg catctagtac aagaagtgtt tcttggggtg cttctggtaa
     2821 ttcccgctgg attactcgtt ttattttttc taattcgttc atcaagttca ctttattttg
     2881 cagtcggcct gcagtatcaa ctagtaaaac atctgcctgc tctatctttg ctctttccaa
     2941 agcatcgaat acgacagctg ctggatcgcc gcctgcattg ccacgaacaa cttcaacacc
     3001 agcgcgttcg ccccagacaa ctaactggtc gattgctcca gcgcggaagg tatctgctgc
     3061 tgccatcaat acttttttac cttcgttttt gaactgatgg gcaagtttac caatacttgt
     3121 tgttttccct acaccattaa caccgacaaa caaaataact gttagtccgt cttcttggat
     3181 atttagttca tttacttcat tgattccttc cgcttcatag agatccacaa gtttctcgat
     3241 gatcgcattt tggacttctg ccgatttttt cgcatttctg agtttcactt cttgacgtaa
     3301 tgcttcggtg attcgtatcg ctgtttcaaa acctacatct gcaccgataa gtgtttcttc
     3361 taattcttca aaaaagtctt catcgactgt tcgaaaattg gcaaacaact cattcatccg
     3421 ttcaccaaat gttttacgcg tcttttctaa acccttgtcg tacttctcct gtacttcaac
     3481 ttcttctgtt ttctcttcgc cagtaaaagc acgcttgatt ttatcaaaaa atcccatttg
     3541 actcccccct actctaatac aaatttttgg atcactttgg ctacgccatt ttcttcattg
     3601 ctggctgtca cgaaatctgc tgcttctttg acgaaaggca cagcattctc catcgctacg
     3661 cctagtccag cgtattggat catcggtaaa tcattttctt catcaccgat tgccatgatt
     3721 tcttcttgtt tcaaaccaag atcttttgcc aataaagaaa taccataagc ttttgtaaca
     3781 ccttttggca taaattccaa aagcatactt ctcgttttga tgatctcata catttcatgg
     3841 aattctgctg gtatttcttt gattttagga tctaattctt cttggtcata agcgattact
     3901 gccttattaa gcacaaattc atctgtaata tcgcttatat ccgctggttg gaattgcagc
     3961 agcttattca acacattgta caaggattgc ttccttgggg cactaggcag ctgtaaaacc
     4021 acattgtcag acaaaacatc tagtggcaga tccaattgct gagccagttt gtataagcgt
     4081 tggatatctg atacttccat cactttcttt tccatgattt caccagtatc atttttttgt
     4141 accaaaccgc cgttgaaagt gatgctgtag tcaccttcat ctaccagtcc taattcttgc
     4201 agataatgca ccatcgcagc taaggggcgt cctgtacata gaacgatctt gaccccttgc
     4261 tgttttgctt gtgctaatgc ttgtttattc tcatctgaaa tcagcttttg ttcatttaat
     4321 aatgtaccgt ccaaatcgat tgctactaat ttgatcattt tcgttcctac actttcgttt
     4381 cgttgatttt cgcctcttca ttgatatctt ccatccgtac agaaacgatt tttgatacac
     4441 cggattcctg catcgtcact ccgtacagta catctgctgc gaccatcgtt cctttgcggt
     4501 gtgtgactac gataaattgt gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt
     4561 tcacgttggc ttcatctaac gctgcttcta cctcatctaa gatacaaaac ggaacaggac
     4621 agacacggat aatagaaaag agcaacgcga tggctgtaag tgctcgttct cctccagaaa
     4681 gcaaacttaa actttgcagt tttttgccag gaggttgtac ttcaatctca atacctgttt
     4741 tcagcaaatc agttggttct gtcagtacta attcagcttt accgccgcca aacatatttg
     4801 gaaagacaat tttgaattct tgacggatag cttcaaatac ttctttgaat cttgtccgca
     4861 cttcatcatc catctcatcc atcgtttcaa acaattggtt tttcgctgct aacagatcat
     4921 ctcgctgtgt tgctaagaat gtatgtcttt cactgacttg ttcgtattgt tcgattgaat
     4981 ttaagtttac tggtcccagt ttttcaatct gttcttttaa ggatgaaacc tttgttctac
     5041 tgtcttcgat atcggttgtt tcttgatagt cttgggaagc tttctcaaat gtcagctgat
     5101 attctgactg cagataggtc aaatgattat ctaaaatcat ttcagctcgg tctttttgga
     5161 cttctaattt ggattgttcc gacagtcgtg ctttttgctc acgattacgc tctgccaaga
     5221 ctgcttcaag cttatcgatc tccttttgcc ttctatctcg ctgctctttg gcttttgcca
     5281 actcggcttt caatgtttct cgctgagcag ataattcgtt aatttgtttt tccaaacttt
     5341 cttctgtgac ttcatgatca gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg
     5401 cttcttgacg ttcggttgcc tctgcttttt ggactcgggc accgcggagc tggatctgca
     5461 gatgattaaa ctgctctttc aagacagcaa gatctgcttg ttcttgtgct ttttgacttt
     5521 gtacttgtgc tcttctggct tccatctggt cgctttcttg acttaacgat ttgatttctt
     5581 cgtcgatttt ttgtcgttgc gattcaagat ctgtttgttt gtctttcaat tcgttcgttt
     5641 gcttttcgta atcttcgata aactgctgca gttcacgtgt ctcaaaagtt gagatttgct
     5701 tttctttttc aaaacgttca agttcattta tgatgttttg cagctgattt gtcgcttctt
     5761 gttcttcaaa acgtaattgt tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac
     5821 gagctgtttc ttgttgcagc tcctgaactt ttttttcttg tgcttggagt tgtttatctg
     5881 cttcttcaaa ttcactagtc aactgtttca attcttgatt ttgtacaaac aaactgcctt
     5941 ggtttcctcg tttattcgct ccacctgtca tggatcctcc ggcattcatc acatcacctt
     6001 ctaaggaaac cactcgatat tgatagcgga tcgtttgcgc gattgcattg gcactagtta
     6061 agtctttggc taaaaggatc gttcctagca ggttttgaac cacagtttgg atttgatcgg
     6121 gataactgac ttgttcactg gcaatcccta taaacccttc tacagctgct gcttgtgtca
     6181 atatatgagc cggtaattgt cttggtttga ttgtcgtcag tggaaggaat gttgctcttc
     6241 ctcctctttg ctgtttcaaa tacgtgatgg cttgccgagc atctttttca ttttcaacga
     6301 tgacatgctg ggctgcccca cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa
     6361 catcgatcag ttcagccact gctccgacaa tacccgaaag ctgctgttta tgctgcaaga
     6421 ccaatcgaac cccttggtaa aaaccaaaat agttttcttg gatttcttga aggcttttct
     6481 tacgagcacg tagctgctgg acttgattca ttaattgata cattttaggc tgttcttttg
     6541 ctaatgcttc ttggaacttt ttcccgtttt gttggatcat ttctaacttc tcttttgttt
     6601 cagttaaaga tgattttagc tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca
     6661 atcggtctac tgatgcctct acttccgatt gcttggctaa cgtctgctta ctttttgctg
     6721 tttcttgtac atattgacgt tctagatact tcagttcgtt tcctacagct gcttgctctt
     6781 gcatcaaatc aacatattga tctcttagtt ctgccagtaa ttctttcgtt gatttttggt
     6841 atttttctaa ttcttgctgt gtttttatga gacttgcttc agctttttgg atttctgttt
     6901 ctttttctgc tacttctttc aacaagcttt cttgaagctt ttcaaaatgt ttaacctttt
     6961 tttgtgcttc tgctaatgaa gtttgatatt cttgcgagct tttttgtgta tgctttgtcc
     7021 gctcctgcaa aacatctttt tgtccttctg tctgcttcag tttttccgaa agatctaata
     7081 atacttgctg atttttttcg atcagacgat cggcttgcgc attttcttta cgctgcttcg
     7141 ccaaaatgga ttcttgttct tggatcgact cagatagttt gcctaattct agattgaatt
     7201 tcgcaagctg tgcttgtttg ttgtcccaat ctttttttgc tgtcttgatt tcggcaacca
     7261 tcaaagagac atcggtttga gtcagtgttt cttttagccg taaaaactcc tttgctgcct
     7321 cactctgggc ggccaaagga gttaactgtt cttccaattc gtggatgatg tcttggacgc
     7381 ggctgaggtt gtcttctgtt tcaaaaagct tctgttctgc tttctttttc cgctgcttgt
     7441 atttcaagac accagctgct tcttcaaaga tcccacgacg gtcttccggt ttactactga
     7501 aaatcgcttc tacttttcct tgagaaatga ttgagaacga ttcttttcct aatccagagt
     7561 ctaagaacaa ttcttggata tcttttaatc tgcagctttg tttattgatg aaaaactcgc
     7621 tttccccagt ccgtcgataa cggcgggtta cactgatctc attgtactcc aatggcagat
     7681 aatgatcact attgtctaaa atcactgtga cttctgcaat attcagctgc ttacgggtat
     7741 cggaaccggc aaagatgata tccggcattt ttccgcctcg cagactttta gcagattgtt
     7801 ctcctaatac ccagcggata gcttctgtaa tattgctttt tccactgcca tttggtccaa
     7861 ccactgctgt gacgctattt tcaaagtcaa tgactgtctt gtctgcaaat gatttaaaac
     7921 cggctatttc aattcgtttc aaatacaaag ctttccctca cttactttta cagtgctgcc
     7981 aatgcatttt ccgccgcagc ttgttctgct aattttttcg attttccttg tccagtgcca
     8041 atcaattgat catctacata aacttcgatc caaaatactc gttcatgagc tggaccttct
     8101 tcattgatca atcggtattc gattgataca tcacctgatt tttgcaagac ttcttgtagt
     8161 tttgttttat gatccatctc atgtgaaaaa gcacctgctt tgactttagg aaaaatcact
     8221 ttttcgatga atgcatgggt cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa
     8281 gcttcgaata ggtcgcaaag caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc
     8341 ttgcccaaca aaacatattg atcaaagtga cattctttgg caaatttcga caagctgtct
     8401 tcgcgaacga tcgcagctcg tgtttttgtc aattttcctt ctggcaaatg aggatataaa
     8461 cggaataaat actgagaaac catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc
     8521 tcattatccg ataattgcag attgcgatgc tcattcacat aggatgaatg ggtaaaagct
     8581 tgttcaagca aatttagatc gtgaaaaacg atgttatagt tttcttttag ttcttttgtc
     8641 agctggttgt ccattttcca cattcctttt atggatattt tttcgatact tccttattat
     8701 actggttttt tgtcaaattt gaaatggttc ggtcaaaagg taagtaaagc tttaataatc
     8761 ctagtgattg acgagaagtt gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt
     8821 tgtgaaaatg gctttccata tttttgcata aatttgactt aataccgaga aactggactt
     8881 tgaacaccgt ttattggaga agtcatgaaa tcagcgcctt gacctgaata ttagagaaat
     8941 aaacagaaaa atatcaaaaa accgaaaaag gacatcgccc tttttcggtc atttttagtg
     9001 attatgaacg tgaatctgct gtaacagaaa catttgccca tgggtttggt acatcataag
     9061 accaatccca gtctttgaca cggtcgttga ctggcaatac ttcattacgg aacaacgttg
     9121 gaatcgcaaa tgcttcttca aatgcatatt gttgccattt ttgatatgct tctttttggt
     9181 actcttcatc aaatgctttg cttgaactga tatcgttgat caattttgta ttttcttctg
     9241 attcataacg agtgtagttg aacgcagcat ttggtcccca tagtccactt ggatttggat
     9301 cagttcctgt attccaagca gcttggtaaa catcaatttc aggatcatca ttttctaatt
     9361 tatcatagaa agaattgaat tcgatcagac gacctgttgt gtattggaca tccaaaccga
     9421 tttctttcca ctgttggata tagtaatctg ccaatggttg cgctgtttcc ccaccggaca
     9481 tcgaagcaaa gttgattgtt agtttatttc catctttgtc ttctcttaaa ccatcgtcat
     9541 ccacatcttt gtagccagca tcatctaata gttttttcgc tttgtctaga tcttgtgtga
     9601 atcctttcat ctcatcatca tggtatgact ggaatactgg aggaatcaat gaagtcgcat
     9661 tgcttctcaa acctgcatag aaacgctcac ctactgcatt attatctaat gcatagccca
     9721 ttgcttgacg taatgattta tcagccattt ttgaatctgg attgtattcc acactctttg
     9781 tatctgcatt ccatttacct aacttgaatc ctagataagt ataagaagtt tgttcacggc
     9841 ctaagtttgt atagccagca atttctttat aggtatcgta agtatctgtt ggcatttgga
     9901 aaatcatatc atataattta gagttcaatg cttcagttgc acttgccatc ggtacagatt
     9961 tcatcgtgat ctttttcaat ttaggcgtac cattccagta gtgctcgttt ggcaagtatt
    10021 ctactgattc tcctgcaacg atattactca tgtaataagg gccaaaagtc actggatttt
    10081 tacgtacttg atcgcttgat tccatatctt tgattgctac accttctaac gtatgttttg
    10141 gtgctgcata tgaccaaata ccaccacccg attgaagcat gcttgggttt acttctttat
    10201 attcgatttc aactgttttg tcgtctattt ttttgattcc ggagattgtg tcggcacttc
    10261 catcatgata ttcttccata cctacgatgt tacggaaagt gtcgtcataa cggataccag
    10321 tgtaatcttt attcccaatg atttcgtaag ggaaaatcac atcgtccgcc gttacatctt
    10381 caccatctga ccatttgacg ttgtctttta ctttgattgt cgcttttttc gtatcttcat
    10441 ctagatcaag cgttgcagct ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata
    10501 gcggttcatg agaaggagcc ataaaagctg catcatatga atcttggtaa aactcccact
    10561 ggaacaatcc tttaaactga ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga
    10621 ttgcatcatc attatttttt gttgcaattg gaaaagttaa ttcctctttg ttattgccgt
    10681 tgcctccaga gttgtttcca gaggtatctg tacctccgcc cccgcatgct gcaagaacga
    10741 cagcagacat cagcgctgca aatccaaaaa tcgttttctt cattcctatt tcccccttcg
    10801 cttctatact agcctaaccg ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca
    10861 taattgatac tcaacatcaa aactaaaata aggattgatg cgggtaacca gatccattgc
    10921 cgattcacta agacttctcc gttacttgca taaccgatca atgtacctaa actaggtaca
    10981 tttgccggca agccgaaacc taagaaagtc aaagttgtct cgataccgat gtttgccgca
    11041 aagttcatcg tcaaattagt aatgattaat gaacttagat tcggcatgat ttcgcggaac
    11101 atgattttaa aatcattggt tcccattgtt tttgaggcac tgacataatc tcttcttgct
    11161 tcagataatg tcttgcttcg gaataatcgg gctttagcta cccaataaaa tgcactcatg
    11221 atgaatataa aggaccagac attgtatctg gaaataatcg atacgaagac gataatgatc
    11281 agcatgatcg gtagaatcat gataaaatca acgatccgca tcaagatgtt gtcaatgatc
    11341 cctccgtagt agccagagat gatccctaat ccaaccccga tgatcgaagt aaggatcgtg
    11401 atcgcaaatc cgatcgtaac agagtttcta gcacctatga tcaattgtcc taatacatca
    11461 cgtccgcctt catctgctcc cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt
    11521 gcagcgtatt tgtcgaagat actaacatac atcactttgt cttgatcggt cagcatcgca
    11581 ccgataaaga tcactaataa taaaaccact agcaaaccta atgaaaaaat agcaatttta
    11641 tcttttttaa attctcgagc aatcattcgt atccccatag gaggaatact ttcttgaact
    11701 gctgtattgt ttttttcact catcgatcca ttctcctttc tgattggttt tattgaatcc
    11761 gaatacgcgg gtcaacgata ctcatgatga tatctgaaag taatgtcccc actagcgtcg
    11821 cgattcctaa aatcaatact aaggatgtga tcacggtata gtcccgttgg ccgatggaat
    11881 caataaatag ttttccgact cccggataac caaaaatctt ttcgataact acagaaccag
    11941 caatcaacga agtgatttca taccccattt gagaagccat cggcaacgct gcatttcgga
    12001 aaatatgtcg actgaatact ttattcgtcg gtaccccttt ggaacgggct gttctgacaa
    12061 aatcgagcga tttcgaatcg attacttcac ttcgcagata ttggattgtg acggcagtcg
    12121 ccaaaaatgc ttgggtaatt gatggtaaca gaatatattt caaacggtct atccattgag
    12181 tgataaatcc accttgcaaa cctagtgttt gtgatccact tgttgggaac cagcctaaac
    12241 gataaccaaa aacaaatagc atgatcaagc cgaagataaa taaaggaaca gcaaaactaa
    12301 agaagttgta gattacgaca atcttatcca agattgaatt ttgataccgg ccagcaagca
    12361 tccccagagg cacagcaatc aaataagtaa ggacaactgt caagatcgat agatataaag
    12421 tgttgatgat ccgttcgccg atcaagtcag caacagagcg tttgtataag aaactttgac
    12481 caaaatcgcc ttgcaaagca tttccgatcc aacggaagta ctggacatac catgggtcat
    12541 tcaagccagc tgcttgtcgc atctgttcga tcactgccgg atcctgattt ggattgatca
    12601 aaccggtaaa tggatccccc ggcatcgctt gggctaataa gaagatcaga acacttaaaa
    12661 taataacttg cgggatcatc aataaaacac gtcgcagaat cgttttccac attagcttgc
    12721 acctccatct ttcaacgcca ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg
    12781 accagtcgca tcatagtatt ctttttggtt ttggatatac tcttgttcta ctcttcgtcg
    12841 attttctttg tggctttccc tattttctac atcgatttgc ggaatagctg aaagcagacg
    12901 tttcgtatag atatgtcgcg ggtcattata aatgtcttct cttgtaccga tttctacaaa
    12961 tcgacctttg tacataattg cgatattgtc gcacatgtgt tttactacac ctaaatcatg
    13021 tgaaatgaat aaatagctga ggccgtattc ttgctggatt cgtttcatga aattcaatac
    13081 ttgcgcttga acagatagat ccagagcaga tacaggttca tcggctacga tcagttttgg
    13141 attggtagca acagcacggg ctactcctag acgttgacgt tgtccgcctg aaaattcatg
    13201 aggatattta tatagcgcat cttccggcat cccaacgata tctagcagtc cttttacctt
    13261 tttcttttct tcttgatcgc ttaaccgttc aaaatttcgg atgggttcag caatgatatc
    13321 caacactcgc tttttaggat tcaaactgga cattgaatct tggaagatca tctgcacatc
    13381 cttgttataa cccatttttt tgcggttgct tcgcttcgtt acatcttttc cttcatacat
    13441 gatttgtccg gctgtcacct tttctaatcc cacaacggct tttcccgtag tagattttcc
    13501 agagccagat tctcccacta aaccatatgt ctttcctttt tcgataataa aatccacacc
    13561 atctacagca tatacatgat ccgttactcg attgaagaat ccactgcgaa tagggtaatg
    13621 aactttcagg tctttgattg tgattaattc tgtcatctac acctctcctt tttcgtctct
    13681 aaagtggaaa tgcttatagc aggagcaacg taccaagtgg ttcggtgcga cttcatgaag
    13741 gataggctct tcttcatgat cgcttgctgg tatccatgga atacgtggtg caaaccgaca
    13801 acctttacga ggcatgtttt tcagagaagg aacgacacct tcgatcacat ggagttctgc
    13861 atcatgattt ccttcttgtg gtatagagtt cagtaaagag cgtgtataag gatgcttagg
    13921 atgatcaaac agttctttta cacctgctac ttcaacgaac tgtccgccat acatcactgc
    13981 cactcgatca gccatttctg ctacgactcc taagtcatga gtgatcaaga tgatccctgt
    14041 ttttgtttct tcctgaaggt ctttcagtaa atcaagaatc tgtgcttgga tggttacatc
    14101 tagggctgtc gtcggttcat ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc
    14161 gatgatgaca cgttgtctca taccgcctga tagttcatgg ggatattggc gtccaactct
    14221 cgcaggatta ggaatcccta cttgttctag caattctagt gcacgagctt gacgctgttc
    14281 tgcattcatc tttgtgtgat aggaaagtcc ttctttgatc tggtcttcga ttctcatgag
    14341 aggatttaat gcagacaacg gatcttggaa aatcattccg atatcatttc cgcggatttt
    14401 attatagagc gtttcgttca ggtcgattaa attcagatcg ttatataaaa tctcacctgt
    14461 aatttttgtg ttattaggat caagtaatcc cataatcgta gtagccaagg tacttttccc
    14521 gcacccagat tccccaacga ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc
    14581 atctactgca tcgtaatagt catctttgag tcggaatcct gtatgtaggt tctgaacttc
    14641 taataattgg ttatcggaca atcgaatacc cctctctgct tcctaaatgg aagtttttct
    14701 taataaaaaa ggacattaca cagaataaag cgttttataa caaaggttat tcttatcttt
    14761 gtgtaatgaa ttattaataa ttatatagaa aatcgcttta ctttgcaaca taatttctgt
    14821 ttatcgccag aattatcaga aaaatgactt ttacgtgtca aaaagattaa aaaaagaagg
    14881 ataaaaagct gtatgcagct ttttatcctt ctttgctttt atttgaaatt atttaagatg
    14941 gctgctgata taatctactg cgccaccgac tgtttcaatt tgttcagcgt cttcatctga
    15001 aatttccgta ccgaattcat cttcaagctc taaaacgaat tccataatac taattgaatc
    15061 agcttttaaa tcatctttga tgttcaattc atccgtcact ttatcggtat ctatctcaaa
    15121 atgatttgaa ataattttcg ctactttgtt aaatacttct tcgcgagtca actttttcac
    15181 ctccaagaat catacacttg tcaattacct acaccatggc taattttact ggcatttttc
    15241 aggaatgtcc accttttttt caatggaacg gattgcttca gatatccaaa tactattctt
    15301 cgcttttttc aaattgttcg actaattgtc caactacatc tgtctcaagc attgtgtgga
    15361 tttggcggat cgtatagcga acagcatcgg ggcctgttgc tccatgagtt ttaatgactg
    15421 gtgctttcaa gccaaacaaa acggcaccgc catgtttgga ataatccatt tctgctttca
    15481 aagaacgtaa gccgtctttt aaaagcatag cgcccatttt tcctttgatc ccttcattta
    15541 gaatagcaga ttttaataga ttcatcatat tcattgctgt tccttcaata gatttcaaaa
    15601 cagcgttgcc tgtaaatcca tctgtgacca ctacatcagc cacaccattg agtagctcac
    15661 gagcttctac gtttccaata aaattcaagc ttgtttcatt ctgaaggagt tcataggctt
    15721 ttttcgtcac ttcactacct tttgttgctt ctgttccgtt gtttaagagt gcgacacgag
    15781 gacgtccgat acctcggact ttacgtgcat aaaaagaacc taaaataccg tattgcaaca
    15841 aatgttctgg tttgttttct gcgttagcgc caagatccag catatcgaaa ccggcacctt
    15901 ctttccctac tactggcaag gtagacatca agccagggcg ttcgatattt ttgatacgtc
    15961 ctacgatgaa caaccctgct gctagtagcg cgcctgtatt ccctgctgaa aatacagcat
    16021 ctgcttcacc gtttttcact gcttgtgcag caaggaccat tgatgcagtc ttttttcgtc
    16081 gaattgcttt tactggctca tcatcactat tgattttttc gtctgtatgg ataatcgtga
    16141 tgtttttttc atttgttaag tatttcttga tttctgcttc ttttccgtaa agtaagaatt
    16201 ctacttctgg aaaatcttgc tgcgctaaag ccacgccttc tacaattgct tgaggtgcgt
    16261 gatctccgcc cattgcatct acggcaattt tcattgtttc cactcctttt tccgtcatca
    16321 gtataaagta tagcacagtc tgctaatcga aataacgatt atcctgattt tctatttgga
    16381 ggctttgcgc taaaggctga tactcaggaa gcatctgcca atcctttatc tgccaaaggg
    16441 ctgcagcctc ttctctggct acctccaaaa catttccatc cgtcaccaag tcagcaatcg
    16501 caaattgagg cagaccagat tgacggaagc caaatacttc accaggacca cgtaattcca
    16561 gatctttttc gcttagtaca aaaccattgg tggtctctgt catgattttc atccgttcca
    16621 cacctagttc atttttagga ttagcaacta agatacagta ggaagcatca cttccgcgtc
    16681 caacacgtcc acgaagttga tgcagctgtg ccaatccaaa tcgatccgca tccatgatca
    16741 gcatgacggt cgcgttcggc acgtttaccc ctacttcaat tacagtagtc gaaactaata
    16801 tctgcatctg gttgtctttg aaggcttcca tgattacttc tttttcctga ttcttcattt
    16861 taccatgtaa caagccaact tcatattttg gtgcaaatag ttcacgaagt ttttcataga
    16921 tttccactgc attttttaca tctaatgctt ctgattcttc aatcagcgga cagatgacat
    16981 acatttgatg tcctcttgcc agttcttttt tcgtccaatc cagtacactg tccaattgag
    17041 gtgttcttac ccagcgtgtt tcgatcggga tacgtccagc tggaagttca tcaatgatag
    17101 atacatccat ctcaccatat gctgtaatag ctaatgttct aggaatcgga gtcgctgtca
    17161 taaataagac atctggtcgc cagccttttt cacgaagtac tttccgctgg ttcacgccaa
    17221 aacggtgctg ctcatccgta ataactagac ctaaattctg aaattcgact ccatcttgga
    17281 tcaatgcatg tgtgccgatc acgatgttaa tttccccttg ctctagtttt tcaaggagct
    17341 cacgacgttc ttttgctttc gttgaccccg taagcaatgc gatggttact tcattgggat
    17401 caaacagctg ttgcaagctt tccatatgtt gttgtgccaa gatttctgta ggcaccatca
    17461 acgcaccttg gaaacctgct gtaacggtag cgtataacgc aatcgcggca accactgttt
    17521 ttccgctacc tacatctccc tgcaaaagtc gctgcatatg ctgcggactg cgaagatccc
    17581 gacagatttc attcgttaca cgtttttgtg catttgtcag ttcaaaaggt agcttttgag
    17641 taaattcttt caatttttgg acatcataaa ggatctccaa accattcgtt tctgcttttt
    17701 ctgctttttt caatccttga agacgcattt gaaataaaaa gaactcttca aaaacgacac
    17761 gtcgtttggc ttgatggttt tcttcgtggt cttttggaaa atgcatactt cttacagcag
    17821 aagctcggtc aagcagtcga tatttctcca caaggttgtt tggcagattt tcttcaatca
    17881 atgtgccgta ttcttgaaac ccttttttga ttagatcaac taatgtcgtt tgacgaattg
    17941 atttgttgac gtgatatatc ggcgaaaagt catccattgt ttgagaacct aaaatcttca
    18001 tcccgttcaa tgctttacgc tttgcatccc atttaccgta tacggcgatt tcctccgata
    18061 aaatgacttt ttcttttaga taaggttgat taaaaaaaga aacgttgaac acatcatgtt
    18121 cttgcatcat acgaaattgc aatcggcttt ttttataacc aaatcgattc atcacaggag
    18181 gcgagactac gattcctttc aaagtaacct tttcttgatc ttggatctcg tttagatttc
    18241 tttcctgaat atcttcgtag cggaaaggat aataagtcag taagtcttca atcgtttgga
    18301 tgcccaagct ggcaagacta gctgctcttt tttctccgac tcctgccagc atagtgattg
    18361 gatcatctaa agaacgcata aatttttcct ccctccctta acaaaaaaag gacttggtca
    18421 gcgatcatac ctgatcacta accgaaggcc ttttatgaat gaatactgtg ttagaaagat
    18481 attctgacac agaaattatt cagcagaaaa tagataagga tagacaggct gatctccttg
    18541 gtggatctcg atttccaaat caggatgttt ttctgttagt ttctcagcaa attcatttgc
    18601 ttgttcaact gttccttcat cacctgccaa gatcgttacg atctctgtgt cctcattgat
    18661 catttgttct agtgtactta acgctgttgc tgtgatatct gggttagaga tcaggatttt
    18721 tccatcgatc atgcccaaaa agtctccttc tttgatctct acaccgtcaa ttgaggtatc
    18781 tctgatcgcg tgagtgactt gaccgctcac aacgctagac aacatttctg tcatcgtttc
    18841 tttgttttct tctaaagaag cttgatcatt gaatgaaagc atagctgtca atccttggga
    18901 aatcgttctt gaaggaacaa ctgctactgg gatatctgct acttctgctg cttgatcagc
    18961 tgccatgaag atgttcttat tgtttggcaa gacaatcact ttatctgcgt ttacttcttg
    19021 aatcgcttta acaatgtctt ccgtacttgg attcattgtt tgcccaccac tgataacata
    19081 attagcgcct aaactttcaa acagctcttg gacgccttta ccagcagcga tcgctacgat
    19141 accatacggg atacgtggtg tttctttttt cgcaaactcg ttcacttctt catcgtgttc
    19201 caaaatcgtc tcatgctgca agcgcatgtt gtcgactttg attttgacta atgaaccgaa
    19261 tttttgtcca taattcaaga cttctcctgg gttttctgta tgcacgtgga ctttgatgat
    19321 ctcgtcatca ttgaccacaa gtaaagaatc tcctatacca tctaaatagt tacggaattc
    19381 ttcgtaatcg aatgtactat cgactgtagg tccttctccg atttttacca tgatttctgt
    19441 acagtaacca aaatggatat cttctgtcgc catttgacct tggacactgc gatgatgttc
    19501 tgcattgacc atatcatcca tttctgctgg agaaggttca tatgtttcat ccgcttggaa
    19561 ttctccattc aatgcactta aaaagccctc ataaacaaac agcagtcctt gccctccact
    19621 gtctacgaca ccgacttctt ttaggactgg aagcaagtct ggagttttag ctaaagcacg
    19681 ctttgcccct tttacaacag ctgtcattac ttcaacgaca tcatcagtac tttttgcttt
    19741 cttttctcca gctttcgctg cttctctagc tactgtcaaa atcgttcctt ctactggttt
    19801 cattactgct ttatacgcag tttcaactcc atgagtgaag gctgcagcaa gatcggaagc
    19861 atttaatgag acaacatctg gaatttgctt agaaaatcca cgaaacaatt gagacaaaat
    19921 aacgccggaa tttccgcgcg cacccatcag caatcctttt gaaagcattg ctgctaattc
    19981 tcctactttt tctgaggttg aatctactac tgccttagct ccactagtca tagatagatt
    20041 catatttgtt cccgtatcgc catctggcac agggaataca tttaatgaat tgacgtattc
    20101 tgcatttact tgcagacgat tcgcacctgc ctggaccatt tcctggaact ggcttgcact
    20161 tatttctgtt acactcacct agataatcct ccttcagatc gactgctgag ttaccagcca
    20221 gtctcctgcc tagtctggca gtacacgaac accttgtaca aagacattaa ctgaattggc
    20281 agttacgcct aataatgcct ctagattgta tttcactttt tcttggacgt tgcgggaaac
    20341 ttcagagatt ttcgtaccat agctgataat cgtataaaca tctacagcaa taccattatc
    20401 ttcttggcgg acaactactc ctcttgagta attttcttta cgcaaaatct cagtaatatt
    20461 atctttaatt tgatttttgc tggccattcc tacaacaccg aacacatctg tcgcggcgcc
    20521 accaacaact gttgcaatta catcattggt aatttcaatg gttccagctg acgtttgaat
    20581 ttttacagcc atgaatgagc ctccttaacg ggtaaaaaac tcccattttt cttctatcat
    20641 attttatcat agcgcaactt gaaataaaag gcagttaccc aaagtactca aaaaccttgc
    20701 aaaatttcag gtataacctt gtttaattta caattattct tctcaattgt caattgtatc
    20761 tatgaaaatg tacttgcaaa tttccattct atatgatatg ttagtctggt atgagctgaa
    20821 ttattctatt gctccaaatc aaggcaaagg aggaaattac catggcaaaa gtttgttact
    20881 ttacaggtcg caagacaaaa agtggaaaca accgttcaca cgctatgaac tcaacaaaac
    20941 gtactgttaa acctaactta caaaaagttc gcgttatggt cgacggaaaa cctaaaaaag
    21001 tttgggtgtc aactcgtgct ttgaaatcag gaaaagttga acgcgtttaa ttgaatgata
    21061 cttggaccgt tcagatagtg aacggttttt tatttaataa aaatttttaa gggtgggact
    21121 tttgattcct accctttttt tgcgtttatt tttgttatac gaacaaatta tgaagttatt
    21181 tattctaaat tttatttttt tgtgaaaatt agactctttt taagcagaga aaaacaagga
    21241 tatgataaac tgaatacaac ttcatagaaa tttggtggaa aataaatatg aaaaaaaatt
    21301 taaagtcatt ctttagagaa aacggacgct acgcagcagc tagtttcctg attccgtttt
    21361 tgattttagc acttgtttat ctaagtatcg ggatctatcc tggaagcagc agaagtattt
    21421 tggcaagtga tgccttttca cagttttcta attttcatgc cagcttccga aatgttcttc
    21481 ttggaaagca aagtattttt tatacatgga atgcttctct aggattgaat tatttatctt
    21541 taatttctta ctatctaggc gggatcttca caccactggt gttctttttt ccaaaccaga
    21601 tgatgccaga tgcactgtat gttctgactt tgcttaaggt cggctgtgcc ggattggctt
    21661 tttggtttct tgcacgtacg ttcaagattc ctcgctgggg gcatgttgca cttagcgtca
    21721 gttacgcatt gatctcattt atcactgcac attcggagat catcatgtgg ctggatgcat
    21781 ttgtttatct tccattagtg attttgggga ttcatcgtgt tatggatctt aaacggccga
    21841 aacttttatt tatcagttat ttactcttgt ttttatccag tttttatatg ggttttatga
    21901 ttggcgtatt ttctttctta tattttatcg cccgtctatt gacaaactgg tcagaatata
    21961 aaaaaacgat tgttccttat ggtatcactt ctttactggc aggcggcgct tcaatgatca
    22021 ttgtattgcc agcagtgctg gatcttcggt caaatggcga ggcactgagt cagatcacta
    22081 cgttgaaaac agaagcgact tcttatctgg atctaatcat gaaaaatatg attggtgttt
    22141 atgacacgac aaaatacgga tcgattccgt tcgtttacgc aggtctgcta ccattagtcc
    22201 tctgtctgtt ttattttgcc agtcggaaag taccgttgaa aaataaattt ttgtacggca
    22261 gtcttttcgc catacttatc gcaagtttct atcttgttcc attgaatttg ttctggcatg
    22321 gtatgcatgc accgaatatg tttttattcc gttatgccta tctgttctct ttcttagtgc
    22381 tcctgcttgc aggaaaagga tgggaaaaga tagaacaaga cgatcgtggg ctgttgttag
    22441 gaatcatttt ggtcgtagct gctctctttg cggtggcatg gggcatcaaa cctgaaggaa
    22501 gctacaccta tgtgacgatg acttcctttg tattgacagt ggttttctta gggatttatg
    22561 cttttgctgt gggattttat cagcataaaa aactttcatt caatcatctg agtatattgt
    22621 tattattgat catgtctgct gaagcactgg tcaatacgaa cagtatgatc catggcattc
    22681 ttgatgattg gaattatgct tcccgcagtt tatatactga tccttatcct gacttgaaag
    22741 ctcttgtaaa tgaaacaaaa aaagattccg actcgtttta ccgcttggaa aatctggatc
    22801 cagtatctgc aaacgattcg atcaactatg gttacagtgg tatcagtttg ttttcttcta
    22861 taagaaatcg taattcttcc tcttacctgg ataaattagg tttccgttca agagggacaa
    22921 atttgaatat ccgttataac aataatactt tgctaatgga tgggtttaca ggaatcaaat
    22981 ataacatttc taaaaacgaa agcacgttca gtaaatatgg cttccgccaa gctggtcaaa
    23041 gtggcgacta tacactgtat cagaatgtca atgcattgcc actagcattt actgcaccgt
    23101 tgtccgttaa tgatgttgag cagccagtga cagataattt gaccagtcag acaaacttga
    23161 tcaatacatt gtctggttta aatgaacagt attttacatt ctatacacct actttgaaga
    23221 atcagttgaa tacgacgatc acaaagacaa cgactggcgt cacttactca gaaacagcag
    23281 ataaccagcc aaaagagctc acttgggaag tccatgtgcc tgcaaataca caagcttact
    23341 taagtttgtt cccaaccaat tttgcacaat tagaaagttc tagtgcgacg atcactgtca
    23401 atggtacatc gcgaaaaacg caaatcaaca tttctggtca atactatgat cttggctatt
    23461 atcctcaaga gacaacagtg acatttactg caagttttta tggaacgaaa gaagtaagtt
    23521 tcatggagcc taaagtcgtt ggtctagatg ttgtagccta tcaagccgca atgaatcaga
    23581 tcaaagaaaa aggtgtcgaa atggaagcca ctggcagaaa agcttctggg actgtaaata
    23641 ccgctgaaga ttccatgttg gtgacaacta ttccttatga caaaggatgg aaagctaaaa
    23701 ttgatgggaa accagtcaag gtcagcaatt tcaaagacgc attcttgatg ataaaagttc
    23761 cagcaggtaa acatactgtc actttcagtt acttgcctga aggatttacg ataggtgcaa
    23821 tcctttttgt cctatgtatt gcattgttta ttctttatgt ctggtacctt gaccgcaatc
    23881 gcccctttta tggcacgatt gatccagcaa tagaagaaaa accgcgaaat acacacagaa
    23941 gaagacgaag aaaataaaga aacgatggaa tctgtgtcac tcttctattt aaatggcaaa
    24001 aatttcaatc tttaaatgac aaaaaccacc tgatttttgg tggtttttta tatacctatg
    24061 actaaactat ctcttgctgc ttcgacaact tctacagtga tcttatccat ttgattgatt
    24121 cttaaaatcc gatcaatctg cgcaaataaa cgatgaataa gcctaaaatt tccttttgtg
    24181 atcttaatga tagttgtgat agcctcataa tcagaaaaat cttctagctt gatatcaatt
    24241 cctaaatctg cccatttgta ttccaaaata tgatgtgttt cgtccttact aaggttatca
    24301 aattcatgtg caaatcctat gcgagagtaa agctgcggat aacgagacaa tcttttttca
    24361 ataccaggca taccgataag aaccattgcc agatcatttc tatcgtaaat atctcttagc
    24421 tgttctaaat gctgaagctt taaacgatca atttcatcga caataattaa gtcaattgct
    24481 tcataggttt taggcacagg aggatcttct tgattgtatt ttagtaccat atgcattgct
    24541 ttagtcagac ctattctact agttattgtg ttaatatccg aactgacacg agacatcttt
    24601 tcagctggta cagtataaaa aactttattt acacttaata tttcatctgt tgcatttatc
    24661 aagaacaaga aaaagtctga atattcaaaa aataacggtg tacattttta aaaggagatg
    24721 tataatcgag atttagaaag cgttttccta aaaatttaat gaattaggaa gtgattacta
    24781 tgaaaaaatt atttagcgca ctttttattc tgttatttgg ctttattggt attgggactt
    24841 ctggaaatat agttaaagct agcacgatgg ttgaacaaac aagtgaaatg gcaaaaactt
    24901 ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc caaaggattt agtgataaag
    24961 atacttttta cactcaaaag atagagcctc aagatccgaa aacacgtttt tctgcaaatg
    25021 tattgcggtt aaccgcatct actaaaaaaa ttactaatac gcaggggcgg acagaatata
    25081 ttataacaag tcccaattcg gcgatgtatc gtatttctac acgcttgagt ttaggtagtg
    25141 caaaatcata tactcaaaca gtaaatctag ctaattctta aggttatggt ggaggaatgt
    25201 attttacatt tactgggaaa agacaatatt acagtgtaaa attagctatg caagtttata
    25261 atacatttgg tgtagggtca gtctcttgca ctgcgggagg attgacaata ggaaaatgat
    25321 aatttcttac tatgtcttaa atagaagtga tgatgaagga ctcttcgttt ttcaatcatt
    25381 acatgaggat acgatgctaa aagagtttta taatcggatt aagaataaac agatagaaac
    25441 aaatttatat tttttaagtg actctatctt gccttttatt agcaacccta aaattcttaa
    25501 cttgttgcag acaaaagatt tgaaaagttt accgattatt gtcattgatg aggaaattgt
    25561 aaaatatggc gcttatctaa gcgtagaaga attagaaaaa ttaactaaca ttggaatgag
    25621 tattcaggag gataatgggg tacatggaac atagaatgaa tatgcacagt tgagaacgga
    25681 atttgacatt tatttagacc aacagggtat accctgttgg tctttttgtg cgttcatgat
    25741 atacttggtg tcatttttat atttaaatgg cacaaatatt tctaattatt tgtcatttaa
    25801 cgctgaaaat ttttgctatt tatctaaaaa agtgacagga tactcatttg gccttcatag
    25861 aattttctaa atatgaatca tcaatgaatt taattaaaaa aataagtagg gcaaaatata
    25921 aatgtttttg ccttacttgt ttcc
//
LOCUS       DACEQH010000037        25695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.037, whole genome shotgun sequence.
ACCESSION   DACEQH010000037 DACEQH010000000
VERSION     DACEQH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.037"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            127..3654
                     /locus_tag="IW833_001657"
     CDS             127..3654
                     /locus_tag="IW833_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1307780.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="IW833_001658"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="IW833_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ1307781.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="IW833_001659"
     CDS             7442..7783
                     /locus_tag="IW833_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1307782.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="IW833_001660"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="IW833_001660"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1307783.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="IW833_001661"
     CDS             9140..11560
                     /locus_tag="IW833_001661"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ1307784.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKEINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IW833_001662"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IW833_001662"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ1307785.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="IW833_001663"
     CDS             12994..13731
                     /locus_tag="IW833_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1307786.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="IW833_001664"
     CDS             13745..14560
                     /locus_tag="IW833_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1307787.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="IW833_001665"
     CDS             14573..15220
                     /locus_tag="IW833_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1307788.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="IW833_001666"
     CDS             15236..15892
                     /locus_tag="IW833_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1307789.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="IW833_001667"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="IW833_001667"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ1307790.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="IW833_001668"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="IW833_001668"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ1307791.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="IW833_001669"
     CDS             18234..18752
                     /locus_tag="IW833_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1307792.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="IW833_001670"
     CDS             18859..19359
                     /locus_tag="IW833_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1307793.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19639..20559)
                     /locus_tag="IW833_001671"
     CDS             complement(19639..20559)
                     /locus_tag="IW833_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ1307794.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20724..21737
                     /locus_tag="IW833_001672"
     CDS             20724..21737
                     /locus_tag="IW833_001672"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ1307795.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="IW833_001673"
     CDS             complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="IW833_001673"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ1307796.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22822..23262
                     /gene="fabZ"
                     /locus_tag="IW833_001674"
     CDS             22822..23262
                     /gene="fabZ"
                     /locus_tag="IW833_001674"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1307797.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23377..24138)
                     /locus_tag="IW833_001675"
     CDS             complement(23377..24138)
                     /locus_tag="IW833_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1307798.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24327..25421
                     /locus_tag="IW833_001676"
     CDS             24327..25421
                     /locus_tag="IW833_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1307799.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25507..>25695)
                     /locus_tag="IW833_001677"
     CDS             complement(25507..>25695)
                     /locus_tag="IW833_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1307800.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT         8510 a         4455 c         5415 g         7315 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aagaaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
    19561 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
    19621 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
    19681 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
    19741 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
    19801 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
    19861 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
    19921 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
    19981 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
    20041 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
    20101 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
    20161 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
    20221 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
    20281 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
    20341 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
    20401 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
    20461 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
    20521 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
    20581 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
    20641 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
    20701 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
    20761 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
    20821 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
    20881 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
    20941 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
    21001 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
    21061 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
    21121 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
    21181 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
    21241 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
    21301 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
    21361 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
    21421 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
    21481 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
    21541 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
    21601 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
    21661 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
    21721 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca
    21781 ggaagagact ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac
    21841 aaaagtttat gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct
    21901 ggaaagtggg ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg
    21961 ctgatcaggc gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt
    22021 gacagctaat gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga
    22081 gaactcagct gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat
    22141 gttataattc gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt
    22201 tggtgcaaga aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc
    22261 ttgagctaat tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt
    22321 gttggcgtaa gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc
    22381 attcattgct ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc
    22441 ttcgccgacc aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat
    22501 ttctgcccct tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc
    22561 gacgcccata atgacgattt tttttccttc tagaaatgac ataatgactc tcctttgttt
    22621 ctgataaaaa tttgatagtc gtaaaaaaat agagaataat agctaaaata acttcttgat
    22681 ttcttaatta ttagtcaaaa tgttttgatt gtcaaactat tttttcttaa attaaaaaaa
    22741 gattgctaaa tatttaggaa tatgatactt tgatagtcaa agtaatgaaa atggcaattg
    22801 cctgaaagaa ggagtttaat tatgtcttac gtattatctg ctactgaagt aatggaattg
    22861 attcctaatc gttatcctat ttgttatatt gattatgtag atactttaga accaggaaaa
    22921 aagatcgttg cgacaaaaaa tgtaacaatc aatgaagatt ttttccaagg tcactttcct
    22981 gacaatccag tgatgccggg tgcgttgatt ctagaaacac tggcacaagc aggttcgata
    23041 cttatcctaa aatcagaaga gtttctagga aaaagagctt atatcggtgg gatcaataag
    23101 gccaaattcc gtcaaaaagt tgttccagga gatgtgctga aatgtcattt tgaaatcacc
    23161 aaaatgaagg gatcagtcgg tacagcgtta tctgcagcat atgtagatga taaaaaagtc
    23221 tgtgagtgcg agttcacgtt catcgtcaac caaaatgctt gactataaaa cctgatatag
    23281 caaaacaaaa ggaagcttac ttgcttttta tgtaaaaaga agacgcctga atcctagcat
    23341 acgttgtcat ctatgcagga ttcagacgtt atttttttat ttttgttttg cttcttgcag
    23401 tatattgatt ttatcgattt ttacttcttc ttttggtttg tctgaactat ctgtttcgat
    23461 atttgcaata gcatccacga catccattcc ttcaatcact tggccgaaga cagtataatg
    23521 gccatctagt gaaggataac cgccgttttt ataagcgtcg atgattgctt gaggatagtc
    23581 gtcttttaaa agaccatcat gcatatcatc tgtattttga acgataaaga actggctgcc
    23641 gttactgtta ggatcttgtg agcgagccat agataacgca cctcttatat tataaaggtg
    23701 gttgttgatc tctgtttcaa atccttctcc ccaaatgctt tcccctccag tgccatcgcc
    23761 tttaggatcg ccaccttgga tcatgaaatc tttgatgacg cgatggaaag tcagtccgtt
    23821 gtagtaaccg tcttttgcat gtgtcataaa gttttcaacc gcttttggtg cgtgttcggg
    23881 aaataatttg attttgattg tgcctttatt cgtcaccatt tcgaccagat cttcattttc
    23941 ggcgacatct ttcgtcaatt gtggtaattc caatgcgttc aaatcgacgc tactggcagt
    24001 cgtgctttcc gcagtagatg tactttttga actttctgta gacgaactag aagaagtcgt
    24061 gttgtttgca gaacaaccag cagcaaacag caatgctgct gctaaactaa tacttaaaat
    24121 aactttattt ttcttcataa ctgttccttt ccaatttact attttgttat ctttaatgat
    24181 aacaaaatag agggaaaata cctacttaaa ttattggaat ttatcattta ttttaaaaga
    24241 aaaagaaaag gtgtagacca tttactttgt gtttgctata atgtaagtgg atacaaaaac
    24301 gaaaagatgc aggaggaaat ggtaatatgg gaaaattagg aatctcgatt tatccagaac
    24361 ggtcaacatt tgaaaaggac aaagcgtact tggatttagc tcataagtat gggtataaac
    24421 gggtttttac cagtttgctg cagatcaatg atgataaaga aaaagttctt tcagaattta
    24481 aagaagtagt tgattacgcg aatagcctag gcatggaagt aatggttgat atcaatccag
    24541 cgttatttga acagttggag atttcttacg atgacttatc ttttttccat aagatgggtg
    24601 cctacggggt ccgcttagat atcggattca caggagcaga agaagcaaaa atgacgcgca
    24661 atccttttgg tatcaaaatc gagatcaata tgagttcggg cacgaattac gttgacaaca
    24721 tcatgagtta ttcgccaaac acggataatt tattaggttc tcataatttt tatcctcatc
    24781 gatactctgg tttaggatac gaacactttg ttttttgttc agaaaaattc agaaaatata
    24841 atttgaatac gatggcattc gtcaattcgc aatctgctga gtttggccct tggcctacgc
    24901 aagatggctt gtgcacgctg gaagaccacc gagatcttga aattgcgaca caagtgaagc
    24961 atttaatcct tacaggattg attgatgata tttctattgg aaatgcgtac gcaagtgaag
    25021 aagaattgaa agaaatggca gaagcattta acgcggatta tccaacattg aaagtagata
    25081 cggaagaagg tattacagaa aacgaacgaa tctgcttgtt tgataacctt catagttatc
    25141 gaggggatcg ctcagaatat atccttcgtt caacgatgac tcgtgtatac tacaaagata
    25201 aagatttccc gcctcataat acgcgagata tgcatcatgg ggatgtactg attgacaatg
    25261 aagggtacgg tcaatacaaa ggagaaacac aaatcgcctt gaaggatatg aaaaacgacg
    25321 gacgtgtcaa tgttgtagga agaatctccg atgacgaatt attcctcctt gactttttga
    25381 aaccatggtc aagctttaaa ttaattgaaa ataaaaaata gataattagt tattgcagaa
    25441 ataatccgct agaaatagcc agttatttct gcttttttat tttgtttttt tataggttat
    25501 gatttgtcaa attaagctag aataaaatgc ttcttgtaca tagctcaaaa atatttcgat
    25561 tggtgttctg taattcaatg attttcttgg aatatgatta cgttgattgc tgacactgga
    25621 aataaatgtc tgattcactt ttctaaaatc cattgatttc ggcagtccat tacgacgcag
    25681 aatcccgtta gaatt
//
LOCUS       DACEQH010000038        25675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.038, whole genome shotgun sequence.
ACCESSION   DACEQH010000038 DACEQH010000000
VERSION     DACEQH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.038"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            170..514
                     /locus_tag="IW833_001678"
     CDS             170..514
                     /locus_tag="IW833_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ1307801.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="IW833_001679"
                     /pseudo
     CDS             515..934
                     /locus_tag="IW833_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1026..1376)
                     /locus_tag="IW833_001680"
     CDS             complement(1026..1376)
                     /locus_tag="IW833_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307802.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="IW833_001681"
     CDS             complement(1342..2673)
                     /locus_tag="IW833_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ1307803.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="IW833_001682"
     CDS             2929..3495
                     /locus_tag="IW833_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1307804.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="IW833_001683"
     tRNA            3640..3727
                     /locus_tag="IW833_001683"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            4061..5035
                     /locus_tag="IW833_001684"
     CDS             4061..5035
                     /locus_tag="IW833_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1307805.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5360..7255
                     /gene="asnB"
                     /locus_tag="IW833_001685"
     CDS             5360..7255
                     /gene="asnB"
                     /locus_tag="IW833_001685"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ1307806.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7450..7896)
                     /locus_tag="IW833_001686"
     CDS             complement(7450..7896)
                     /locus_tag="IW833_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1307807.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8124..8273
                     /gene="rpmG"
                     /locus_tag="IW833_001687"
     CDS             8124..8273
                     /gene="rpmG"
                     /locus_tag="IW833_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1307808.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8361..8879
                     /locus_tag="IW833_001688"
     CDS             8361..8879
                     /locus_tag="IW833_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1307809.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8956..9912)
                     /locus_tag="IW833_001689"
     CDS             complement(8956..9912)
                     /locus_tag="IW833_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1307810.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10078..10884
                     /locus_tag="IW833_001690"
     CDS             10078..10884
                     /locus_tag="IW833_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1307811.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10881..11870
                     /locus_tag="IW833_001691"
     CDS             10881..11870
                     /locus_tag="IW833_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1307812.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11867..12871
                     /locus_tag="IW833_001692"
     CDS             11867..12871
                     /locus_tag="IW833_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1307813.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13003..13545
                     /locus_tag="IW833_001693"
     CDS             13003..13545
                     /locus_tag="IW833_001693"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ1307814.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13565..14263
                     /locus_tag="IW833_001694"
     CDS             13565..14263
                     /locus_tag="IW833_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ1307815.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14284..14496
                     /locus_tag="IW833_001695"
     CDS             14284..14496
                     /locus_tag="IW833_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ1307816.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14496..15458
                     /locus_tag="IW833_001696"
     CDS             14496..15458
                     /locus_tag="IW833_001696"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ1307817.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15476..15871
                     /locus_tag="IW833_001697"
     CDS             15476..15871
                     /locus_tag="IW833_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1307818.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16034..16399
                     /locus_tag="IW833_001698"
     CDS             16034..16399
                     /locus_tag="IW833_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ1307819.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16396..18207
                     /locus_tag="IW833_001699"
     CDS             16396..18207
                     /locus_tag="IW833_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ1307820.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18269..18436)
                     /locus_tag="IW833_001700"
     CDS             complement(18269..18436)
                     /locus_tag="IW833_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ1307821.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18684..19673
                     /gene="galE"
                     /locus_tag="IW833_001701"
     CDS             18684..19673
                     /gene="galE"
                     /locus_tag="IW833_001701"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ1307822.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19685..21169
                     /gene="galT"
                     /locus_tag="IW833_001702"
     CDS             19685..21169
                     /gene="galT"
                     /locus_tag="IW833_001702"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ1307823.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21193..22173
                     /locus_tag="IW833_001703"
     CDS             21193..22173
                     /locus_tag="IW833_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1307824.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22262..23086
                     /gene="tsaB"
                     /locus_tag="IW833_001704"
     CDS             22262..23086
                     /gene="tsaB"
                     /locus_tag="IW833_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ1307825.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23070..23612
                     /gene="rimI"
                     /locus_tag="IW833_001705"
     CDS             23070..23612
                     /gene="rimI"
                     /locus_tag="IW833_001705"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1307826.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LGVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23619..24083
                     /gene="rimI"
                     /locus_tag="IW833_001706"
     CDS             23619..24083
                     /gene="rimI"
                     /locus_tag="IW833_001706"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1307827.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24080..25096
                     /gene="tsaD"
                     /locus_tag="IW833_001707"
     CDS             24080..25096
                     /gene="tsaD"
                     /locus_tag="IW833_001707"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ1307828.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         8327 a         4399 c         5379 g         7570 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gtaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 atggacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa
     3781 cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg
     3841 caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc
     3901 ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta
     3961 taaatgctag taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa
     4021 gataaaagtg taaaaacgat ttgttttagg acgggaaacg atgtgtaccg caatcactta
     4081 tgcaacgaaa gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt
     4141 ggtcactatt actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa
     4201 acattatgca atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc
     4261 tacaaatgaa aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa
     4321 agaattacaa gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg
     4381 gcaatgctca accatagacg aagctaaaga attattggct actatcaatc tagtcaatat
     4441 cgattttagt gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa
     4501 atcgatagta attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt
     4561 gttgacaaat aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct
     4621 atcaaatgga acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg
     4681 tgggatggga ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc
     4741 cacatttact aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt
     4801 ttttcatata ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaagaaa
     4861 atacgaatat acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg
     4921 tacgtatgaa gacagtcaaa ttactgctgt aaatatgaat aaagaagact tggatcgtca
     4981 tgaattggtc agttatccag tcataaaaga gcaacagata aactatgtca attaattaat
     5041 gaatagatcc gtttggtatc atattttctg atacagtcat aattagacac gatctttagg
     5101 tcgtgtcttt ttttgctggt ttttttggaa atagcatcca ctaatatttt tttaacacga
     5161 agagaataag gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt
     5221 ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat
     5281 attcgtccgc tatttattct atgataagat gcatatgaat gaaataaaca gtaattttat
     5341 attaggatgt gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa
     5401 aaaaacaatt attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg
     5461 agaatatatc gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga
     5521 gggcggtact cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga
     5581 aatctataat taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac
     5641 acatgcagat acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca
     5701 aaagatccgc ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg
     5761 tgcaagagac cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat
     5821 gtttggttca gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa
     5881 agaagcgttg aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt
     5941 caaagatgtc tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaaataga
     6001 cattcagcaa tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg
     6061 gatcgaaaaa atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt
     6121 agaagttggt tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa
     6181 accagatcat tctttctcaa tcgggtttga tgataaaacc tataatgaag cgattgaagc
     6241 cagaaaacta accgaactat tggacttaga taatacagca gcagtgatcg acggagacat
     6301 gtcgttcaaa gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc
     6361 ttgcgtacct ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc
     6421 tggagaaggg gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc
     6481 aaaattcatc cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa
     6541 tttaggacgt aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc
     6601 acttgctcct gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc
     6661 atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt
     6721 tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct
     6781 tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc
     6841 tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc
     6901 aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa
     6961 acatcttccg gaaaaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc
     7021 ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttgttcgaag cagattttgc
     7081 aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg
     7141 taaagaagag caaagaaaaa tctggacaat cttcagtttc ttgacatggt ataaagtcta
     7201 ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa
     7261 atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca
     7321 atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc ggagttggca
     7381 gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt
     7441 tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa
     7501 agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc
     7561 aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata
     7621 ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc
     7681 cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc
     7741 ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc
     7801 atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttttt
     7861 cattgatgca ggaaagtcct tcatgttcca tggcatatta gtatccccct ttattaaaaa
     7921 acataatcta tataaacaag tgtaagggta tttatacaaa aagaaaaaga ttttgtttga
     7981 gaagtaaaac cgttgtattt atcgtcaagt agttttactt aacaaaatcg tgaaaaacac
     8041 tagcaatcta gaaaaaatca tggtaagata atcaagtctg agagtcatct tagaggattc
     8101 ttgtaaaaat gggagggaaa aacatgcgcg taaacatcac tttagagtgt acttcttgta
     8161 aagaacgtaa ctatctaaca agcaaaaaca aacgtaacaa tcctgatcgt ttggaaaaac
     8221 aaaaatattg cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt
     8281 aaagttctca attccttgct ggtcaaagaa ttgagacttt ttttatctca aaacatctaa
     8341 aatcataagg agcggataat atgtttattt ccatgtgggc acaagataaa aatggtctga
     8401 tcggcaaaaa tggtttgtta ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac
     8461 atacaatgga tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt
     8521 cacttcctta tcgccatatc atcgtgttga caacgcagcc aaattttcgc acgaaagaaa
     8581 atgcggaagt aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg atctcagaag
     8641 atatctatgt ttcgggtggt agtcggattt ttcaagaact tcttccttac actggtcgga
     8701 tctggcgaac attgatcgat agcacgtttg aaggcgatac gtatattggg aatattgatt
     8761 tttcagactt cgcccttata gaggagtatg aggggataac ggatcaagaa aatctatatg
     8821 cccatcgttt tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa
     8881 atctatgcgt ctagtttgtt aataagaaaa aaagcatatc acgactaatc gtgaagatgc
     8941 tttttttagt taaaatcaat ttagtttttc tttgatatca ttgaccattt cctgactggc
     9001 aataggtccg ttatatagcc aactagattc aggtccgaat tgcagtacat gattgtctgt
     9061 cactgcttta agattcttcc agcttggttc attgaacatg gcagcatctt tgtcactgtt
     9121 cacaaggatg atgtagtcag catctaattc agctagcttt tcagaggaaa cagcagacca
     9181 gtcagaagtg gcttttttgg agacctcttc cactagatca ggaacaccaa attccagatc
     9241 gtcgtatacg attcttccac ttgaccgatt tttgctgacc ataaaggctg agttgttggt
     9301 cacccacaac actgcagctg tcttgttctc gattttatct gctagttctt ctcgagtttt
     9361 cttgatggat ttttgataat cggaaatgat cttttctgcc tctttttctt ttgccaaagc
     9421 tttgccgaca tcttttagtt gttcttccca tgtgacatct gttccgtttt ttacaacata
     9481 agttggcgcg attttgctat attgttcgta tttcccgcct tcgacagtag cggaagaaga
     9541 aatcaacagc aaatcaggct caaagcttaa cactttctca taaggaaggt cataagaaat
     9601 cgttgggaca tcctttaaat catcttgcag ataatcttga atggaaccac tgccaacagt
     9661 ccactgggcg ataggttttt cccctagtgc aatcagatag tcttctaagt aactgccgat
     9721 gatccgtttg ggcgcgtttg gaatctctac tttatgtcca agtgtatctg tgactgtatg
     9781 agtactattg gatgaagtcg tcgaattggc agttgtagct gtttctttat ctgaagaggg
     9841 ggagcaagcg ctcagtactc cagccagcag caatatgcta gataatgtga atacttttgt
     9901 ttttaatttc atttctttcc tcctaacata attgagaatc tttctcattg gttattattc
     9961 ctgatatttc gaacgctgtc aaccgcttca tttggctata gatatgaaag tagcgcttga
    10021 cgaaatgttg aaacagcgtc ataattgaga atgattttca gcgaaaggat gggtgaaatg
    10081 acgagattaa gagcagctga cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac
    10141 atgtctgtta cgattcctga agggaaaatc actagtttga ttgggccaaa tggaagtgga
    10201 aaatctactt tattgaaaag ttttacccgt atactacctg tgaaatcagg aaagatcatt
    10261 gtcaataact ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa aattgcatta
    10321 ttagcacaaa ctagtgaaca ccctacaggt atgacagtag aagaagtagt atcttacgga
    10381 cgataccctt atcaaaaact gttcagtggc atggacacgc aagatcatga agcaatcgag
    10441 tgggcgttgg atgctacagg tctagctgga tttcgtatgc gggatatcgc aacgctgtct
    10501 ggtggacaaa cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac agatatcttg
    10561 atcttggatg aaccgacaac atttttagat ccggcacacc aattagagat cctacagtta
    10621 ttgactgaaa tcaatcaaat gaaacaaaca acgatcttga tgtcgattca tgacattaat
    10681 catgcttcac gtttttcaga ttatttatta gcaatgaaaa acggtaagtt gatcacagaa
    10741 ggtacacctg aagaagtgat cacaaaagag tgtatgaaaa aaatatttga gattgatgca
    10801 acgattgtca cgcttccaag aacaaataaa ccagtttttc taacctatga tttgttgcca
    10861 aaagagcagg aggatcaatt atgaggacta tgaagaaaaa tagactcctg cttttggtct
    10921 gttttgttct tttgattatt ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa
    10981 aagcagattt ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt caccaaatca
    11041 tacgaaatat tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt tgttttgcat
    11101 tgagcggtgc attgatgcaa ggagtgacaa gaaatccttt agcagattca ggcttactag
    11161 gaatcaacgc gggtgcttcg ttggcaatgg cccttagttt tgctttttgg cctaaagctt
    11221 ctgatttttc tgtgtttcta atgtcattgg caggttcttt tgctgtaact ttgctcgtat
    11281 ttggtttttt gcagttttcg aaaatgggca tgaatccttt acagctgatt ttagctggcg
    11341 tagctattag ttcttttttt actgctatca gccaagcact tacccaattc tttaatctgc
    11401 agcaggatct tgctttttgg tttgtaggcg gagctgccaa tatcacatgg aaacaattag
    11461 cagtattgtt tcctttatat atacttgctg ttttggtttc tttttcatta ggaaaagcaa
    11521 tatctttgat tcatttgagt gaaacacatg cattagctct tggaagtcat cctaaacgaa
    11581 cgcggttgat tgttttctgt ctagttgcag tgttagcagc cattgccgtt tcgttagtcg
    11641 gacctgtttc atttattggg ctgttggttc cgccagttgt tcgcggaatg gttggaacgg
    11701 actatcgtta tgttctacct gtctcttttt tagttggcgg tggattggtt atgttggcgg
    11761 attttacagc gaggatgatc aatccccctt ttgaaacacc ttttggatta gtcattgcat
    11821 tgattggcgt tccgttttta ctgatacaag taaggaggga acaacggtga aaaaaatata
    11881 ttggacgttc tatttcttgc tttttgtact tgtcatcctg tctataggta gtttaatgat
    11941 tggtacgcct tttatttacc tggatcagct ttttgccacg ttcataggga aagaatcatc
    12001 ttctttggcg ctgattgtta gagaatttcg agttcctcga ttactgatca gtttattagc
    12061 aggagcttgt cttggcgttt ctggttatat cttgcaagga gtaacaagaa atgaattggc
    12121 agattctagt atattgggca ttaatgccgg agcaggattt ttagtgatgc tttatttagg
    12181 tttcttttcg cactattcgc tgccgtttct attgctagtt gtagcctttc taggtggtgt
    12241 tttagctgct tgctgtgtct atcttgcggc ctatgatcga aatggctttt tgggtatgaa
    12301 tcgaatctta ttgtccggta ttgccgttaa tgccggcctt tctgcattaa cattgctatg
    12361 cacgatccaa ttatctaagg aaaattatgg atttgtgaat gcatggttag cgggatcaat
    12421 ctggggtgcg agctggcctt atgtttgggc attgcttcca tgggcggtta tcttaattcc
    12481 gctaggagga ctttatgcac agaggattgt attgctatca tttggtcaag aacgcgcgca
    12541 aggattaggc cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt
    12601 ggcaagcggt agtgtagcgg tgacaggtag tttatctttt gtaggactac tagctcctca
    12661 tgctgcgaag ctagtcgtac gtaaagaaaa taactggacc tttgttctga gcggtttgtt
    12721 cggttccatt tttctgctta gtgcagatat ggttgctaga gtgattctac taagtgggga
    12781 agtacctaca ggaattttga ttgccctttt tggtgcgcca tattttctgt atgcactttt
    12841 tatcagacaa aaacacgcgc tgaacagttg aaattcatta aaaaaggaag gccaatcatt
    12901 tctaggtctt ctttttattc tatttatgca tttatttgaa agttcgattg ctatttttct
    12961 ttttttccta taccatttta tgagagaagg aagggtgagt ttttggacca attgaaagta
    13021 agattacgcc ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag
    13081 gaaaaagaac agcgaatcta taaacagcta ttcgccagcg agtactggaa gtcagcaaaa
    13141 gtgataggga cgactttatc gaatgaattt gaactggata cacagccggt tatccaacga
    13201 gcagaaaaag aacaaaagaa aatagttgta cctaaaactt tgccgaaacg tcagatggcg
    13261 ttttatgaaa tggatgaaca gtccgttctt gtacgttcga gttttggtgt tcttgagcca
    13321 caatcagaga agctctattt gccggagatg atcgatctcc tcattgtacc aggtattatc
    13381 tacaatagag ccggttatcg aatcggtttt ggcggtggtt attacgaccg gtatttaaat
    13441 aattttcata aaaaaacatg tagccttgtt tttcgtgaac aattgaatga acaatggcaa
    13501 ccggagagct ttgatcagaa gatccaacaa ttgtttattg actaaaatga aatgggaggt
    13561 tttcatgaat tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac cgttatggac
    13621 ctatgctttt ttagggatac agacagcggt cttcctcatg atggaattat ttccgcgact
    13681 tgagatccct tattatgccg gaatgtatgg tccttatctt gttcacttca atgaatggtg
    13741 gcgtttagtg acaccaatct tcatccattt tggactgatg catttcgtaa tgaactcact
    13801 gatactgtat tttatggggc agcaaatcga ggcaatctat ggccattggc gttttttctt
    13861 gatttatatg ttcagcggga taatgggaaa tacagcaagc tttgctttta acgaagcgaa
    13921 tgttctgtct ggtggtgcaa gtacttcgat attcggtttg ttcggtgcgt tgtttatctt
    13981 agggtttcat tttaaacata acccagcgat ccagcagcta gtcagacatt ttcttttatt
    14041 tattgtgttg acatttgttt ttggtcttct ggatacctct gtagatgttt ggggacatgt
    14101 cggtgggcta attggcggat tagtgttagg gaatgttttg ggtctaccga aacaacagac
    14161 aagctattcg atacaccaaa ggattctttc tacactagtt tttgtatttc tttttgtaat
    14221 atgtatttta ctgggtttaa aaaaatatgg tttacttgta taatatgaag gaagtgctta
    14281 aagatggaga gcttatatga tgtacagcag ctgttcaaaa aatttggcat ctttatctat
    14341 gtgggtgcac gcatctatga cattgaatta atgactatcg aattgcgtaa tctgtatgat
    14401 agccgtttga ttgatcatga tacatacatg acagcgtggc gcatattaaa aagagagcac
    14461 cgaattgaag aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt attgggattg
    14521 atttaggtgg aacaacagca aaatttgcaa ttttaacacc agaaggagag attcagcaaa
    14581 aatggagcat cgataccaat attttggatg atggtaagca tatcgttcct gaaatcattg
    14641 aatcaatcaa tcatcgtttg aatttatata atatgaaagc cgaagacttt atcggaatcg
    14701 gcatgggtac cccaggaagc gtggacagcg aagctggaac ggttattggt gcgtataact
    14761 taaactggac agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat
    14821 tcgctatcga taatgacgcg aatgttgctg ctttaggtga acgatggaaa ggtgctggag
    14881 aaaacgatcc agacgttgta ttcgttactc taggaacagg tgttggcgga ggtatcgttg
    14941 ctggcggaaa tttgattcac ggcgtgagcg gagctggagg agaaatcgga catatcactg
    15001 tggatcctga agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa actgtatcta
    15061 gcgctacagg tgtggtacgc ctaggtcgtt tcttggcaga agaatacgca ggggattcaa
    15121 aactgaaagc aatgctagac aacggagaag aagtaacaag taaagatatc tttgaaatgg
    15181 ctcaagaaga cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt
    15241 tagcttgcgg aaacttgggg aatacattaa atccgtcaag tatcgtgtta ggtggaggag
    15301 tatcggcagc aggagaattt ttacgtagcc gtgtagagaa atattttaaa gaatttactt
    15361 tcccgcaagt aagagaatca acaaaaatca aattagcaga gcttggtaat gaagctggtg
    15421 tcattggtgc tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc attacatggt
    15481 tttatgggta attaatatca ttttgctatt gattgtgctt gcaatcatcc tttgggaagt
    15541 ctatctacga gtaatggcca aacgttcagc aacgactttg acagaagaag aatttcgaga
    15601 aggaatgaga aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca
    15661 tattttaggt gcaagaaaca tcccttacac tgttttgaaa gattcattag gatctattcg
    15721 aaaagaccag cctgtctata tttacgatca aaagaaaagt ttgagcatcc gtacagcaaa
    15781 taaattacgt aaagcaggtt atcaagagat ttatttactt aagggtggat atgacggatg
    15841 gtctggaaaa atcaaaagta aaaaagtata aaacagatga ttgtatgaca gctttaaaaa
    15901 ccgaatcaat cattagattt tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc
    15961 gttcattttt tttattttgt gctattatct acatatataa gctaagcgaa aaggtagaaa
    16021 gaaggaaaca actatgatca aattaaaacg tgtttacctt gatgctgaaa aagaggatgg
    16081 ctatagaata ctcgtggata gattctggcc acgaggaatt tcaaaagaaa aagcgcagtt
    16141 agatctgtgg ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt ttgaccatgt
    16201 accagaacga tttgcttcat ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga
    16261 aaagaagcaa gctattttta aactcagtca acttatgaaa gaacatccag tcattacttt
    16321 tgtttatggt gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa aatttatcga
    16381 aatggaggca tgcaaatgaa aatagggatc attggtgccg gacctagagg attaagtgta
    16441 gcagaaagga ttctccataa taatcaggac agacattctc ttgaactttt tcttttcgat
    16501 cccaatggtc ctggaggtcg agtttggcgg ttgaatcagc caccggagtt attaatgaat
    16561 tctgtttctc aacaagtgac gctttttaca gatgaaacat tgtccagtgg aggattggta
    16621 acacagggac ccaacttgtt tcaatggagt aaaggacctg ctacagatta tatcgaacag
    16681 acggtaacca aaaataagca actaattata gaagaaatca atcgtctgga aaaagatcag
    16741 caatctactc gatgtttata cggactctat caacgctggt ttttctcaaa gttgcaagaa
    16801 gaatttccag cttctattca gtttgtttat tctttagtaa aaaaagtaag caaaagaaaa
    16861 gatggttatc ttttacagac agatcagcaa agctattcta tcgatcagct tgttttagca
    16921 agcggtcact gggaaaatga tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag
    16981 aaaaatgttt tactgtatca gcttccttct aatcctgcag atgtagatct ttcgaagata
    17041 ccgcctaaag aaaatgtctt tttaagaggt ctaggcttaa gtttttttga ttatgtcgga
    17101 ttgtttacag tatcaagagg agggcgcttc gagaacgaaa agggaaaact ggtctatcat
    17161 ccatcaggca aggaaccgat tgtatacagt ggatcaagaa gggggctccc ttattaccct
    17221 cgaggaagaa atcaaaagca aggcggagca atggcatggc cacggttact aacaaatgaa
    17281 aacctggagc aatggcatca gtcgggattt ttgactggcg aaacattttt tgactatctg
    17341 aaaaaagacg ttgagttgtt ttattacaag aaaatcatcg aagaatatca cttgccgatt
    17401 ccaaagaaaa attttgagca tgattttctc tccctctctt cagaggagtg tctcggaaag
    17461 catccagcac taatccctta tagatgggat tggtcgtatt tagaaactcc tcttgcctat
    17521 cagaatgagc ccttttcaga agctagccga atatttattg aagggcagat aaaagaagcg
    17581 gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt
    17641 gatccaatcc ggcaggctat tgaatggcac gttttttctg ctaaggaata caaagaaata
    17701 ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc aatcattcgg
    17761 acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgatcga accgccattt
    17821 gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt
    17881 cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc
    17941 ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga
    18001 agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca
    18061 ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta
    18121 actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata
    18181 gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg ttttttagaa
    18241 agtcttcatg cgtattttta aataattatt aacgagaaac gcgtttacgc attgcttttt
    18301 tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca
    18361 cagaagcaac caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag
    18421 atacaaattt tttcattgaa tccacctcat ttattatttg ttatatctat tatggaacaa
    18481 aagagaaaaa aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa tccttgaaaa
    18541 tgtttactaa atgttttatt ataaatttac catatgaatt tacgaaattt tactttgttt
    18601 gttttcacca taaaagtaaa cttcgttttt taaattatat agaatcaaca aacacaattt
    18661 tactaatcaa aggagtgttt tttatgacaa ttttagtact aggcggagcc ggttatatcg
    18721 gttcgcacgc tgtagaccag ctagttcaaa aggggtatca agtagctgtg gtagataatt
    18781 tgctcacagg tcacaaacaa gcagtacatc cagatgctca tttttatgaa ggagacatcc
    18841 gggataaaga atttctacgt tccgtatttg aaaaggaacc gattgaagga gtaatccatt
    18901 ttgctgcaag ctctttagta ggagaatctg tcgaaaaacc attgatgtat ttcaacaaca
    18961 acgtatacgg tatgcaaatc ttacttgaag tcatgcacga gttcaacgtg aataaaatcg
    19021 tattctcttc tactgcagca acttatggag agccgaaaga gtctccaatc acagaagata
    19081 caccagctaa tcctaaaaat ccgtatggtg aaagtaaatt gatgatggaa aaaatgatga
    19141 agtggtgtga ccaagcatat ggcatgcgct atgtcgcttt gcgctacttc aatgtagccg
    19201 gagcaaaagc agatgcatct atcggagaag atcatacacc agaaacgcat cttgtcccaa
    19261 tcattttaca agtagctctt ggtcaaagaa aagccttggc tgtttatgga gacgattatg
    19321 atacaccaga tggaacatgt atcagagatt atgttcaagt agaagatttg attgctgcac
    19381 atattttagc tttggaatac ttgaaagaag gaaatgaaag caacttcttc aatttaggca
    19441 gcagcaaagg atactcagtc aaggaaatgc tagaagcagc tagagaagtg acaggaaaag
    19501 aaattccagc agaaatagca ccaagaagag ctggagatcc tagccgatta gtcgcttcaa
    19561 gtgaaaaagc gagagagatt ctgggctgga aacctgaata cacagatatc aaagcgatta
    19621 tcaaaacagc atgggattgg catgtcagcc atccaaatgg ttatgaagaa taggggaatg
    19681 agatatgtca atcagtcaaa cgatttgtga ttttgccaca cttgcgatcg aagctggcgg
    19741 atggatggaa atggaccgct tatatttaca gaatcgtctt ttagggatgg taggagaaga
    19801 tgctctagaa agcattgaag taagaccagt taaggaagaa tctgtagact tattggatca
    19861 gcttgtcgct aaggcaagag aaaatggtgt tctttcagaa ttgccagcag aacaagagat
    19921 ttttgaagcg caattaatgg attttctcac accgccacct tcagtcgtca atgcattctt
    19981 tgcacagcac tattcgaaaa gcccagagga agcaactgag tatttcttta atttaagtaa
    20041 gaaaaatgac tacatcaaaa caagagccat cgcaaaaaac atcaaatttc cggcagagtc
    20101 ggagtatggt gaactggaaa tcacaatcaa tttatctaag ccagaaaaag atccaaaaca
    20161 aatcgcagca gaacgcgagg cggtaaaagt agactatcct acctgcatgc tttgcatgga
    20221 aaacgaagga tatagaggtc gcttgaacta tccagcccgc accaatcacc ggattattcg
    20281 aatgaatcta gacggtgaaa gctggggatt ccaatattcc ccttatgctt attacaatga
    20341 acattcgatc attttatcag aacaacaccg cccgatgaaa atcacaaagg aaacatttgc
    20401 tagactgctt cggattactg aagtcttgcc tcattacttt gttggttcga atgctgactt
    20461 accaattgtt ggaggatcga ttctttctca tgatcattat caggcaggac gtcatgaatt
    20521 tcctatggca aaagcaccga tagaaaaact tatcaaacta gaagattatc cgcaagtcat
    20581 cgcaggagtc gtgaaatggc cgatgtctgt gatccgtttg caatcaacag agaaaaactt
    20641 gcttattgaa gcagctaact cagtattgga aaaatggaaa gcatattcgg atgaaacagt
    20701 atctatcaga gctttttcag cagatggtac accacaccat acgattacgc caattgcacg
    20761 aagaagggac aattactttg agttggacct tgtccttcga gacaacaatg tttcagaaga
    20821 acatcctgat gggatttttc atccacataa agatgtccaa catatcaaaa aggagaacat
    20881 tgggttgatc gaagtaatgg ggcttgctgt tttaccacct cgtttgaaac cagaactagt
    20941 agaagtagag catttcttat taggagaaga aaaccagatg gctgaatatc atcgtccgtg
    21001 ggcagaacaa ttaaaaaaag aacatcctga tttgacgaaa gatacggtca ccgatgtcgt
    21061 acaaaaatct gtaggtcagg tttttgcacg tgtactggaa gacgctggag tattcaagcg
    21121 agatgaaaaa ggacaggcag cgtttgaacg atttactgcg attttataaa cgtaaaaaag
    21181 gaggagagac aaatggcaac aattaaagac attgcgcagt tggcaggagt ttctcctgct
    21241 actgtttcaa gagttctcaa ctatgatcct gatctttccg ttggcttagc cacaaagcaa
    21301 aaaatttttg aagcagcaga acgcttaaat tatacgaaac atcgtaaaaa tgcagcaaat
    21361 actaagcaaa cgcttcgcct gatccagtgg tacgacgata cggaagagct tgaagatctt
    21421 tattatttat ctattcgcct aggaatcgaa aaaaaggcag aagaagcaaa cgttacttta
    21481 ttgaaagaaa cgtgggattc cattagtgat cttccggcag atggaacgat cgcactagga
    21541 aaattcgacg aagctcagtt attagaaatg aaaggatccc aaaatgcctt gcttttggta
    21601 gattctgacg gcacttctca aggaatcgac tctctagttg tcgactttaa gtcaagtgtt
    21661 caaaaagtca ttgaacactt tgtctcccat aaagcgcaac atatcgctat gcttgctgga
    21721 acagaatata cgaagaaaaa ccatcagcgg ctaaaagatc ccagaattat ggcatttcaa
    21781 caagttttgg cagaaaaagg ctacgacgaa ggtccaatta tcgaagcaga tttttctgtc
    21841 gagtcaggat atcaagcagt gaagcagtat ttaaaaacga atccgcatgt tcctgatgcg
    21901 ctttttgcag ctaatgatgc attagcgatt ggcgcactga aagcgattca agaaaaaggt
    21961 ttagtggtac cagaagatat ttctgtgatg ggcttcaatg atgtcagtgt agcgaaatat
    22021 gtaacgccct cgctttctac tgtaaaagtt tataccgaat ggatgggaga gctagcagtg
    22081 gagacgattc ttcaattaag taattccaaa gcaccggttt caagaaagat tattgtggaa
    22141 acagagttga ttattcgaga atcaacgaaa taaaagatgg aaaagctgtg ggaaaatgga
    22201 gtgataccgc agcttttttg tgtttcagga aggggcaaaa tttgcccgcc agcagataat
    22261 catgtattat tgtactagtt ttgcctttgg tattttcatc ctcagaataa aaaagtatgc
    22321 tacaatggaa ggcgagaatg tcatagcaga aaggatcaaa tatatgatta cattaggaat
    22381 agatactgca aaccaaactt tggcgatcgg tgtggtagaa gacgaacaaa ttcttggaca
    22441 aatacaaaca aatatcaaac gcaaccacag tgtaacactg atgcctgcaa tcgaccaatt
    22501 gtttgcagat cttcaaataa gtccaaaaga cattgaccga atcgcggttt ctgatggtcc
    22561 cggttcttat acagggcttc gtataggtgt aacgacagca aaaacgattg cttatacatt
    22621 agataaagag ctagtaggag tatctagtct gaaaaccatc gcggctaatt gcatcggtgt
    22681 ggataatatt attgtgccca tgtttgatgc tcgaagaaaa aatgtatacg ctggggcata
    22741 ccgctggaaa gaaaatagat tagaaactgt acttgaagat gtacatattt ctgcttctga
    22801 tttgttcaca caactacaaa agctgaatga agctatttat ttcgtcggga gtgattgtca
    22861 caaattcttc tctgaaataa aggaaatatt gcctagtgcg atgattaata cagttccagc
    22921 atgggatatt ccaagtggtg taactgtggc tcaattaggt gctttagttg aaccagttgc
    22981 agagaaacaa gcttttcttc ctcgctattt aaaacgagta gaagcagaag aaaaatggtt
    23041 ggagacgcac acacctggag acgaaagtta tgttgaaaaa atttagagcg tatgccagaa
    23101 gcttttttca agataaaatg atttatacag aaaaaacagt cgaacaaatc gatcgtcgtt
    23161 acgtactaag agaattgact acagaagata tcaaagagct gctgggtgtc gaacgagaag
    23221 tctataatgg cgaattgcca tggacaaaaa gtgcctttat atcagaattg aagtcaccgc
    23281 ttcctcatct atatctaggt atctttgata atggaagaat gattggtttt atcggttcaa
    23341 gagcgttcgg tttagattgc catatcacga atattgccgt tcgaccggca tatcagcgta
    23401 aacgaatcgg ttcgctgctt attgacgaaa tcgagaactt tgctataatg aatcgctgtg
    23461 agacgatgtc attagaagtg cgtatgagta atcaagatgc acaaagactt tatcggaaat
    23521 tggggtttca agcaacaacg atcaaaaaaa gatattacac tgaaacaaaa gaagatgcgt
    23581 tggatatgat aaaactttta gaaaatgagt gagattagtt gatttgcaga aaaaaagatt
    23641 ttgatgaagc gtacttggca gaatgcgtat ggatgattag cgaaaatgct tatccacatg
    23701 gttcaccttg gacgaaaatg cagtttttgg cagacatcca acagcctcac tctgattatc
    23761 tgcttttagc tgaagataac cagatctgtg gctttattgg atactcgaaa gtaatagatg
    23821 aagtcgagat cacaaatatt gctgttgcag tttcagaaca aggaaaaggg catgcacggc
    23881 atttgttaca attactgatt acggaacaaa taaaagattc gctgcatgtt tttttagaag
    23941 tacgtctatc taatgaagca gcaagaaaac tttatgaatc agaaaagttt cgtatattag
    24001 gtaaacggaa atcatattat cgcaatccga cggaagatgc gatcattatg agcagaaagt
    24061 taaaaacgga gacgatgaaa tgaatgaaga attgattctg gcgattgaat caagttgtga
    24121 tgaaacgagt gtagcggtcg ttagaaatgg aacagagatc ttatctaaca tcgttgcttc
    24181 tcaaataaac agtcataaac gttttggtgg cgtagtacct gaagtagcta gccgccatca
    24241 cgtagaacaa atcaccttat gcttggaaga tgcactggta gaagctggtg tgtcggcaga
    24301 agatttatcg gcagtcgctg tcacttatgg accaggactt gtcggctcgt tgctgattgg
    24361 tatttctgca gctaaggctt ttgcttgggc acaccaattg ccgcttattc ctgtaaacca
    24421 tatggcaggg catatctatg cagcccgtct ggttaaacca ttccagtttc cattgatggc
    24481 acttcttgtt agcggaggac atacagaatt agtctatatg caagaagatg gatcatatga
    24541 aatcattggg gagacaagag atgacgcagc aggagaagct tatgacaaag ttggaagagt
    24601 attaggtctt tcttacccaa gcggaaaaga aatcgatcag ctagctcatc agggaaaaga
    24661 taattaccat tttccgcgag cgatgatcca tgaagataat tatgatttca gttttagcgg
    24721 attaaaaagt gcatttatca atttggtgca taatgcacaa cagcgtggag aagatctaga
    24781 caaaaatgat ttagcagcta gctttcaagc aagtgtgatc gatgtattga ttactaaaac
    24841 acttcgagct tgtcaaaatt atccagtcaa gcaactgatt atagctggag gtgtagcggc
    24901 caatcaaggg ttgcgtgaag gattgcagga tgctttggca actaaactcc ctgaggtaga
    24961 actagtgatt ccccctcttc gactttgtgg agataatgct gcaatgattg gagcagcagc
    25021 acatgtggaa atgcaaaaaa gaaattttgc tagctatcag ttgaatgctg atccaagctt
    25081 ggttttatct gaataaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
    25141 ccaggaaaaa cggcttctca tgtttttgac ctggctgttc ttattaccgc gtatgcaagc
    25201 ttttgaacgc cgtttaatca agagttcgta agcctgtcgt ttaactctga ttcacaagga
    25261 acaatttcaa aaaacagctc ctcatgttta tgaacattat gacttgtgac cgaagagttg
    25321 acaggtacac accgtttaat cgaaataggg ctgtgacacg tttttgtcaa agccctattt
    25381 tttacatact tttaatcatt attgttattt tgctattttt tacagcttat ttatcattat
    25441 ccgttcctct gattgccctc actgagtaat cgtttattgt cgaggaatat cgaataatct
    25501 agatcggatg aggacaagac gcttcatcta gttaatctaa cagtattttt tgcaaacagt
    25561 gaatattaca taactgaaac acacatagaa aatctttgag tcttgtccag tgatctaaaa
    25621 ctgccggatc tttcagaagt tttagggagg aaaataagca gtgaccctga tattt
//
LOCUS       DACEQH010000039        25378 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.039, whole genome shotgun sequence.
ACCESSION   DACEQH010000039 DACEQH010000000
VERSION     DACEQH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.039"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(3..1991)
                     /gene="treP"
                     /locus_tag="IW833_001708"
     CDS             complement(3..1991)
                     /gene="treP"
                     /locus_tag="IW833_001708"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ1307829.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLVYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            2214..2927
                     /gene="treR"
                     /locus_tag="IW833_001709"
     CDS             2214..2927
                     /gene="treR"
                     /locus_tag="IW833_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ1307830.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            3004..3450
                     /gene="msrB"
                     /locus_tag="IW833_001710"
     CDS             3004..3450
                     /gene="msrB"
                     /locus_tag="IW833_001710"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ1307831.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            3620..4222
                     /gene="ruvA"
                     /locus_tag="IW833_001711"
     CDS             3620..4222
                     /gene="ruvA"
                     /locus_tag="IW833_001711"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ1307832.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            4235..5236
                     /gene="ruvB"
                     /locus_tag="IW833_001712"
     CDS             4235..5236
                     /gene="ruvB"
                     /locus_tag="IW833_001712"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ1307833.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            5265..5843
                     /locus_tag="IW833_001713"
     CDS             5265..5843
                     /locus_tag="IW833_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1307834.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKVLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            5895..6377
                     /locus_tag="IW833_001714"
     CDS             5895..6377
                     /locus_tag="IW833_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ1307835.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            6494..6868
                     /locus_tag="IW833_001715"
     CDS             6494..6868
                     /locus_tag="IW833_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307836.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(7668..9020)
                     /gene="brnQ"
                     /locus_tag="IW833_001716"
     CDS             complement(7668..9020)
                     /gene="brnQ"
                     /locus_tag="IW833_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1307837.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            9167..9757
                     /locus_tag="IW833_001717"
     CDS             9167..9757
                     /locus_tag="IW833_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1307838.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(9885..11234)
                     /locus_tag="IW833_001718"
     CDS             complement(9885..11234)
                     /locus_tag="IW833_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1307839.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(11402..12142)
                     /locus_tag="IW833_001719"
     CDS             complement(11402..12142)
                     /locus_tag="IW833_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1307840.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(12155..13747)
                     /locus_tag="IW833_001720"
     CDS             complement(12155..13747)
                     /locus_tag="IW833_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1307841.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(14315..14611)
                     /locus_tag="IW833_001721"
     CDS             complement(14315..14611)
                     /locus_tag="IW833_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307842.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            14787..15512
                     /locus_tag="IW833_001722"
     CDS             14787..15512
                     /locus_tag="IW833_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ1307843.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            15505..16347
                     /locus_tag="IW833_001723"
     CDS             15505..16347
                     /locus_tag="IW833_001723"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ1307844.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKAVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            16350..16871
                     /locus_tag="IW833_001724"
     CDS             16350..16871
                     /locus_tag="IW833_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ1307845.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(17124..17687)
                     /locus_tag="IW833_001725"
     CDS             complement(17124..17687)
                     /locus_tag="IW833_001725"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1307846.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            17938..18207
                     /gene="rpsO"
                     /locus_tag="IW833_001726"
     CDS             17938..18207
                     /gene="rpsO"
                     /locus_tag="IW833_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ1307847.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            18395..20521
                     /gene="pnp"
                     /locus_tag="IW833_001727"
     CDS             18395..20521
                     /gene="pnp"
                     /locus_tag="IW833_001727"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ1307848.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            21031..21297
                     /locus_tag="IW833_001728"
     CDS             21031..21297
                     /locus_tag="IW833_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1307849.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            21441..23432
                     /locus_tag="IW833_001729"
     CDS             21441..23432
                     /locus_tag="IW833_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ1307850.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            23786..25087
                     /locus_tag="IW833_001730"
     CDS             23786..25087
                     /locus_tag="IW833_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1307851.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
BASE COUNT         8173 a         4807 c         5203 g         7195 t
ORIGIN      
        1 gactaagatt ttacttcagc tttaccgact gcttcgcccg ggcgaacaag tcctgattgg
       61 tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag caacaataat gtcagttttc
      121 ttgccagctt gttctatttc tggcaaatcc atttcagcaa tcagggtctc tggtgtgaca
      181 aggtatcctt ctttcacgaa tacattgaaa ggaacaccct ttagttcaac agtatctaat
      241 cccatgtgaa caagtacttc cagcccctca ttagaaagaa taccaatggc atgttttgtt
      301 tcaaagacac tcgtcacttt accgtttact ggtgcataga ctttgccgtt tgacggttcg
      361 acagcataac cgtcacccat cattttttgt gagaaaactg ggtcatttac ttcagttatt
      421 tccttgatgg tccctgcagc gactgcgaat aatgtttctt ttgttgcaac tgccgtacca
      481 gttgctgaaa caggttcgaa agactgtggc gttgctgttg cgccatttgc tgtttgaagt
      541 tgtacatcag cagcattttc aggaacagca ggatcaatct tattcaaaat tccctgacgt
      601 ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa tgatcattgc gatgatgaaa
      661 ggtacccatg tttctgcttg gatagctagt attcctggca aaccgcctac tccgatcgcg
      721 ttagctcgaa cacccatcaa gttagcaaac atgccagcta atccagagcc gaccattgca
      781 gcgacgaaag gatagacgta tttcaaattg attccgaaca tagcaggttc agttacgcct
      841 aagtaacaag aaatcattgc tggaatcgag atttgttctt cttttttatt cccacgatgt
      901 aagaagatga tggctaagac tgcagagcct tgggcgatat ttgataaagc aatcatcggc
      961 cataaattcg ttgatccgaa atctgcaatc agctgcatat cgatggcatt ggtcatatgg
     1021 tggagaccag taatcactaa aggagcatac aggaatccga atacggcact aaacaaccag
     1081 ctgatagaag acgtcaatcc agtatttacg atatttgaga tccagcttcc gactgtccag
     1141 ccgataggac ctaaaataac gtgtgcagct aaaacagttg gtaacaatga aaacaatgga
     1201 acaaaaatca tcgagatcga ctgagggata tatttgcgga agaagatttc tagatacact
     1261 aacataaacc ctgccagcat ggctggaata acttgcgctt gataaccaat catctggact
     1321 tgagcaaaac caaaatccca aaatggaata tctgctgcgg ctgtggaagc tacactataa
     1381 gcatttaata attgcggaga aactaacgta attcctaaaa cgattcctaa tatttgtgta
     1441 gtacccatct tttttgcaat actccaagtg atacctactg gaagaaaatg gaaaattgct
     1501 tcacccggta accacaaaaa ggcattgaca ccattccaaa acttcgagtg ttcaacgatt
     1561 gttccaccaa gactttcaaa ctggatacct tcaagtacat ttctgaatcc aaggatcaat
     1621 ccaccgacaa tgattgcagg aatcagtggt gtaaagattt ctgctaaaac ggcaatagca
     1681 cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc cttgttcttt ggaaacccct
     1741 tcaactccag atactgcaac gaagtcgtta taaaaagtag aaacatcgtt tcctatgatg
     1801 acttggaact gtcctgcatt cgtgaacatc ccttttacgc taggtatatc ttcgatcgct
     1861 ttttcgtccg cttttcctgg atcattcagg acaaaacgca tacgagtcgc acaatgacta
     1921 actgcagcaa tattttcttt tccgccgata tcatgtaaca gcttctcggc atctgcttga
     1981 tattttgcca tatcaataaa cccctttctc ttaacttgtc tatgcaagtt gtttcttcat
     2041 ttactatact ttttttcgga gagagttgca agcgttttat taaaaagtac gagagaaaat
     2101 gatatcgttt taaaaagctt aaaataagct tttcttaaaa ataggaacat tgaaatacaa
     2161 aaagagctgt agtatagtga agaaatgatg gaagaacgag tgagggaaaa aacatgaata
     2221 agttcaatga aattttttta gatttggaac agaaaatttt agcgaaagaa tatcctccgc
     2281 attcgctttt acccagtgag aaccaattga tcaaaattta tggtgtttca cgagaaacaa
     2341 ttagaaaagc gctgaatctt ttgactacgg aaggttatat tcaaaaaaaa caaggaaaag
     2401 gttcgatcgt actggatcgg aatcgcttcg actttcctat ttctggattg actagctata
     2461 aagagctaca ggagacccaa cgtattccaa gtgaaacgat catccatacg ttagaagaaa
     2521 cagaagtgac aaaagcattg aacgagatca ctggctggga gatcggtgcg cctgtttggc
     2581 atttgatacg cgaaagaaaa atcaatggcg aagtagttat attagacacc gattatctat
     2641 taaaggaaat cgttcctatt ttaactccag cacaagcaca aggatcgatc tatgaatatt
     2701 ttgaaaatga gctttctttg acgattgctt acgcgcaaaa ggagatcaca gtagaagaag
     2761 taacggaaat catgaaaaca gcgatggatc ttcacgatga cacttatgca gtagtagtca
     2821 gaagcctcgt tcatctggaa gatacccgct gtttcgagta ttctgagtcg gttcaccgat
     2881 tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa agtgtaaata gttcaaaaga
     2941 aagcaaattg ttggcatctg tccactttgg cgctatgata ggatcacaaa gggggcattt
     3001 ttaatggata aaccaataag accagaaaaa gaagaactga aagaaaagtt atcacccatg
     3061 gcttatgcag tcacacaaga aaatgcaaca gaacgaccat ttagcgggaa atatgatgat
     3121 ttttacgaaa aggggatcta tgtagatatc gtcagcggtg aaccgttatt cagctctgca
     3181 gaaaaatatg atgcaggttg tggctggcca tcattcagca aaccaataac gaaaagacaa
     3241 gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa tagaagttcg aagcaaagaa
     3301 gcagattctc atctcggaca tgtgtttacc gatggaccag tagatcaagg tggtctacgt
     3361 tactgcatca attctgcctc actgaaattc attccatatg aaaagatgga tgaattaggt
     3421 tatggggaat tcaaatcact tgtagaataa tggaaataca caaagagcgg tacaatacgg
     3481 ggatttactc gatcgtccgc tcttttttta ttcaagcgat tgctatttga aagaaacgct
     3541 ccccagactc cttttttcac aaggtgaaat gtgctacaat aaagggaacg tatgtgtgca
     3601 aaagaaagag gactagaata tgtatgaata tctgacaggt gtggttacct ttatcaaccc
     3661 ctattacata gtagtagaaa ccaatggtat cggctaccag atcgcactag gaaatcctta
     3721 ccgctacagt agcaaactaa atcaaaaaat aaaaatttat gtccatcagg taatacgtga
     3781 agatgcgcat cttctgtatg gatttgattc gctagaagaa aagcaactgt ttttgcgatt
     3841 agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca atcatggcaa gcgaagacca
     3901 ctccggtttg attcaagcag tagaatccag tgatgtcact tatttgacga aatttccagg
     3961 agtagggaaa aagacagcac aacaaatgat cttggatcta aaaggaaaat tcggtgagct
     4021 gagtatcgat acacctttta gtctatttga tgaagcaaac acaaaagacg ctacagctct
     4081 atctgaagca atggaagctc tctcggctct tggttacagt gatcgagaaa tcaaacgtgt
     4141 agaaaaacaa ttaaaagaag tagataatca gacaacggat gagtatctac gccaagcact
     4201 caagctaatg atgaaaaaat aaggaggaga ccaaatgaca gatgaatact tgttatcacc
     4261 agaatctggc gaaaatgaac tgagtttgga aaaatctctt cgaccacagc tgctttctca
     4321 atatatcgga cagagaaaag tcaaacaaga acttcagatt tacatcgaag ctgccagaaa
     4381 tcgggaagaa cctttagatc atgttctgtt gtacgggcct cctggattag gaaaaacgac
     4441 gatggccatg gtcattgcta atgagatgac cgtaaatatt cgtacgacga gtggccctgc
     4501 tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa ctggaagcag gggatgtcct
     4561 gtttatcgat gagatccatc gattacctag agtggtagaa gagatgcttt attcagctat
     4621 ggaagatttc tatgtagata tcatggtagg acaagggcca accgcacatc cggttcattt
     4681 ccctcttcct ccctttactt tgattggcgc gacgacacgg gcaggtatgc tttctgcacc
     4741 tttacgagat cgatttggca tcatttctca tatggaatat tatgacgaac aggatctgca
     4801 ggagatcgtt ttacgttctt cggatatttt tcaaacagag atcattgaag aaggggcatt
     4861 ggaaattgct cgccgatcac gaggaacgcc tagaatcgct aatcgcttgc tcaaacgggt
     4921 tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat cgtcctatag cggatcaggc
     4981 acttactttg ctacaggtcg atcaggctgg attggattat gttgaccaga aattgttgcg
     5041 aacaatgatc gagttatatg gcggcggacc agtagggtta agtacgcttt cagtaaacat
     5101 aggagaagag cgtgagacgg tagaagatat gtatgaaccg tatctgatcc aaaaaggatt
     5161 tttaaagcgt acacctagag ggcgaattgc tactgcttat gcctatgaac attttggtta
     5221 cgactatcat atatgatttt tgcttttaga ttggaggaaa atgaatgagt gatacaacac
     5281 gtacgaaaca attgatcaaa aacgtattga ttgatttgtg ccaaacgcaa tcttttcgaa
     5341 aaatcagcgt ccaaaaaatc gcaaatgaag caaaaatcaa tcgacaaaca ttttattatc
     5401 attttacaga taaatacgat ttgcttcgtt gggtctattg tgaggatgca ttgcattatt
     5461 tagatccaca atgtctttca cttgataatt gggaagagca agcattgaaa gtgctgaaag
     5521 caattgctga gaagaaagaa ttttatgcaa atactgtgct ttctgaccca gaaattttac
     5581 aaaaagagtt ttctcaactg atccaaaaat catttatcaa gatttttgaa caaatggatg
     5641 aagaaagaca actttctgca agagacaaac tattttatgc tcgttttttt tcttacggtt
     5701 gcagtggtgt attagtcaat tggatcacga atgaatatga agaaacaccg atggaaatcg
     5761 ctatgcagct tttccgttta gccaaggata cggaattata ttcttatcgt ctttatgcaa
     5821 aagaagagtt ggaagaacag taatagaaaa attttttgtt ggaaaagcaa taatcgctaa
     5881 aaggagatgg ggaaatgaca aaaattttat ttgtttgttt gggaaatatt tgccgttcac
     5941 cgatggcaga aggcttgctg agaaaacgaa tagcaataga aggaagggaa gcagaatttt
     6001 ttgtggattc tgctgctaca agcacgtatg aagttggaaa gacaccgcac cctggaacga
     6061 aaaaaatatt aaaccaagaa aaagtagata tgacgaatat ggttgctcgg caaatcacac
     6121 cccacgattt tgaaactttt gattggatca ttggcatgga tcaagaaaat gtagaagaac
     6181 tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca tttgttttta agtagcattc
     6241 ctggaaaaga aaaagaaaat gttcccgacc catactatac caataacttt gatgagacat
     6301 atcacttgat cacagaaggc ctgaatcatt ggtggaatat ttggattgac gaaaataaaa
     6361 taacaaacaa tttgtaaagt tgacgctttc tttttaaggc gatgttagag catttccttg
     6421 tttttttgta aaatgttagg taaaataaaa ttaattaagt aacactttct tagctaaagg
     6481 aggagtgcat tcgatgaacg aagagacagt atcgccaatg aagaagctta cgaagttagt
     6541 tactcatact tcgtatcaac tttttgattc ttttgtaaat gacactgaag aacatgctaa
     6601 gaatgtacga ttgcaaggaa agtttattct tgagctgcaa aaagctgcta ctgaacatag
     6661 tctagttatc ttacaagtaa gagcgaatcg tttgagtagc aagtatgaaa cgatctcagg
     6721 ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg atccgcctac aaaacgaaga
     6781 gcagcagatc cggatggttc ctgtagaaca aattgaaaaa ataacaatcc tttcaccagc
     6841 aggggaacag gaacgattat caaaataatt tataccagat agaaaagcac gtatgagctt
     6901 gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt taagctgata tcagtaaaac
     6961 ggaacgtttt aggatgagta tcacagcatg acagcattgg gttgattagt tgaatcgatt
     7021 ggatggtaca agtttttttg ataaaaaaag gtcctgctag ccggggagct agcaggataa
     7081 ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg gtatgcttat ataatagtca
     7141 ctagttgtga atattttgtg acgaaagttt tcgaaagttg ttaagttttt ttgcgaaaac
     7201 tatttttata tcatatatat gtctaaaatc tatattataa aagaaatgtt gtttgatcag
     7261 cttctcttcg gtattagttc aaaacaaaac ggcaaagtat atggagctac tttgccgttt
     7321 tgtttctcta atactcaggt cagtttatag acgctcaata aacggtgttc aaaagccagt
     7381 ttctcggtat taagtcaaat ttatgcaaaa atgtgagaag ccattttcac aaaatatttc
     7441 ttaatacttg aaactaagcg gctttttcac gacctctcaa taaacggtgt tcaaaagtca
     7501 gtttctcggt attaagtcaa atttatgcaa aaatgtgaga agccattttc aaaaaatatt
     7561 tcttaatact cgaaactgag cggctttttc acgacctctc aataaacagt gttcaagaga
     7621 cagcctatcg gcaataaggc tgaacgtctc tttcacaacc tcttttatca atctgctact
     7681 tcttctttct tagcgaaact ccaaatcagt ccgataacaa aaccgataac tgctggtacg
     7741 atccacccca tccccaattt gaaaaacggc aagtagcttt ccgcaacata taaaagattt
     7801 tgaacaatag gtgtttgttt gatagtttct gggcaagcgt tcaacccatc aaaaatcgaa
     7861 gcaatcaatg taaaataagt agtcattcga taaacagccg tacgctgctt aaacaaagga
     7921 ccaactaaca ctagtaaaat cagtgtcatt gctaatggat agatgaacat caaaacaggc
     7981 aacgaaaatt gaatgatatt cgtcaagcct acgtttgccg ctaaacaagg aaggatacta
     8041 gcaagtgcaa caaagaatgc gtaatttctt tgcggaaaca aatctgtgaa tgtttctgca
     8101 aaagctgtaa tcaaaccaat accggtcttc aagcaagcta atataacgat aaatgctaat
     8161 aagatactgc cataagtacc taaataatga tcagcaatct gagccagcgt gatcccgcca
     8221 ttttcactta gagcaaagct acctaagctc atagcaccta aataagcgag caaagtataa
     8281 atgattccca tcaatataat gctgatcgca ccagatttga tcgtgtcttt agcgatttca
     8341 gcaggttttg ttacacccat gttttggatt gttgtgacaa tgatgatccc aaatgctaaa
     8401 gcagcaagcg catctaatgt gttatatcct tgagtaaacc ctgtgaaaaa agcatgctct
     8461 tgataatttg gctgaatagg cgcttgattt acagaaccta gtggattcaa aaaagcaagg
     8521 agaagcaaag cacctaataa aaccaaaaat gcaggattca ggaactttcc aacataatct
     8581 aatatctttg tcggttttct tgaaaaccac caagccagca caaaaaatac aatactgaaa
     8641 attgctagat atagcgtctg tctgctagga tcgataaatg gtgctaaacc aatctcgaaa
     8701 gatgtggttg caaggcgagg taaagcaaaa aacggtccga taactaagta aagcaagatg
     8761 gtaaaaacca tcgcatatgt ttgatttatc cgttttgcta attcatatac acctgagctt
     8821 ttcgatacac caatggcaat cacccctaag aagggaagac cgataccggt aaccaagaaa
     8881 cctagattcg cccaaaaggt agcagcgcct gcttcttgac ccaaatgaac gggaaagata
     8941 agatttccag caccaaaaaa tagaccgaaa agcatggagc ctataaaaag atagtcttta
     9001 aatgataatt tttttgtcat acataactcc tttacggttt ttttaaaagt aacaaaattt
     9061 cgaaaaatat gcaacaattt tcagaaaatt tcctatataa aacgacggtt atttaataac
     9121 gtaagtgata aaataaaagc gctgaatcat ctgaggagga atcatcgtga caaagtcgag
     9181 attagaagca tttactgatg gagtggtagc cattgttttg agagttcttg tattagagat
     9241 caagatccct aatacatcta gctttcattc tttatgggtc atccgaaata cattattagc
     9301 ttataccatc agctttatat ttgttgcggt gatctgggtg aaccatcatc gtatatttca
     9361 gatggcagaa cgaattaact accgagtggt ctggtcgaat atctttttgc tcttttggct
     9421 gactttatgt ccttctgtta ctagttgggt cgggcgtaac cctgaagctt tttggcctga
     9481 gatggcttat gtcttagtat acacgatgtg gagtttttct tttggtatct tgatgaggca
     9541 aatcatgaaa gcaaataaac cagattccca tgtcgtgaga gttttgtcaa aggaccgtcg
     9601 aagtcttatc tccatgatga ttaacttatg cttgattgcc ggtgtctttt ttgtaccgtt
     9661 gatcgggtta ttcggacgtt tttttgtatc tggtatttgg attttttcct ataaaaaagc
     9721 agatgcctat tatcagcggt tgtttccggg gaaatagaaa agaggctggg acagaagctt
     9781 ttaactccaa gaaataagcc gaaatttcac aaaaatttaa gaagcaattt tcgtaaattc
     9841 cttcttattt ctcgaagctg accacttctg tcacaactac tttcttactt cttacgttca
     9901 tatttcatat gatagattag taatagaagc aagaaccaca caggtgttag taataaagcg
     9961 attctcgtat cttttcctag gaacaagacg acacacacaa aggccatgta aagaaatacc
    10021 agataattac taaatgggta caacggcatt ttaaatgaat gagctttttt gccgaggata
    10081 cttttacgat atttcatgtg cgtgtagaca agcatactcc agataaacaa gaaacaagta
    10141 gtggcaatac tgcttactag tgtgaatact tcactaggca tcacgtaatt caagataaca
    10201 gaaatgaaaa tgacgatagt agaaaatgtc aaagcattcc ctggcacatg atgtgttgtt
    10261 aactttttga attttttagg ggcactgcct tcttgcgcta aagaacgaag catacgtcct
    10321 gtactatata ttgcactgtt gcaggcagaa gctgctgcac ttaatacgac taaattgatc
    10381 aaggaagcag caatcgtgat cccaatttcc gaaaatactt ctacaaatgg actgttatct
    10441 ggattcaagc tgttccatgg atagattgcc ataatgacaa aaagagatcc taagtaaaat
    10501 aagatgatcc tgataggaat attattgata gcttccggca acactttttc aggattttct
    10561 gtttctccag cgactaatcc aactaattct atcccagcaa aagcaaaggt taccatttgg
    10621 aaagctaaga taaatccttt tgcaccagtt ggaaagaatc cgccatgaga ccatagattc
    10681 gtgatactgg ctgtgccaat atctgtcgta tagtgggtga ggatcatata agctcctacg
    10741 aggataaagg caatgatcgc tacaactttg atcaatgcaa accaaaactc taactcacca
    10801 aacaatgaga cagccaccat attcaagcaa agaagaagac ctaatgcaat cagttcagga
    10861 agccactgag gaacaccagg cgcccagtag cgtacataca ttccgatggc tgttaaatcg
    10921 gccattgcaa tcgataccca gcagaaccaa tacgtccagc cagtgatgaa agccgctttt
    10981 ttgccaagat attctgcaac aaaatccaga aaagaatggt tattggtgtt tgataacaga
    11041 agttcgccta acgcacgcat aatgaaaaaa cagatgcatc cagtaataag ataggcgagc
    11101 aatatcgaag ggccagctag ctggatagac tttccggaac ctaaaaacaa ccccgttcca
    11161 atcgctccgc caatagaaat cagttggaca tgtctatttt tcagtccacg ctgcagttct
    11221 tgctcttcat tcatttttaa atcatcctaa ctaaaaaagt ttaattactt ccatattaaa
    11281 agagttttct gaaaaacaca actaagattc caaaattttc agaaatttac cttttatgag
    11341 taattcataa aaaagaactg cggtaaacac cgcagctctt tgtcccaatc agctattgat
    11401 cctaagcatt taaaatacgt gaaaggaatt cttttgtacg ttcttctttc ggatggacaa
    11461 agatatcttc tggtgtacct tcttctgcaa tcacaccttt atccataaaa atgacacggt
    11521 cagatacatc ttttgcaaat tccatctcat gcgtcacgat aagcattgtc aagccagtat
    11581 gtgctaagtc tttgatcgta ttaagcactt cgccaaccat ttcagggtct aacgccgaag
    11641 taggttcgtc gaatagcaat acatctggat tcattgataa tgcccgagca attgcgacac
    11701 gctgtttctg tccaccagat aattgtgctg gtttggcttc gatataacga tccataccaa
    11761 ctttttccag attttcgatt gctacttttt tggcagtttc tttatctcgt ttcaggacag
    11821 ttgtttgacc agacatacag ttttccaaga cattcatatt gttgaacagg ttgaaggatt
    11881 ggaaaaccat acctagattc gttcgatatt taggtaaatt atatttagga gctaatacat
    11941 tttctccatt ataaatgatc tcgccgcctg ttggtttttc cagtaaattg atacagcgca
    12001 agaaagtaga tttaccagaa ccggaagaac cgatgatcgt cacaacttca cctttattga
    12061 ctgttacgct gatatctttc aatacttcat tttcaccgaa gctttttctt agatgtttga
    12121 tttcaataat tttttcattc atgattcttc ctccttattc ctgcaaattt gaattgtcga
    12181 tttcttcaag cttggtataa gctgaaggac catcgatttt cttttctacc gcccgcagga
    12241 ttctggtgat cgtaaaggtc atgactaagt agatgatccc gacgatcgta aatgtttgga
    12301 agaattggaa attcgcaccg gaagcagagt ttccttggaa gaacaaatca gctacgccga
    12361 ttacacttaa aactgctgta tctttgatat tgataacaaa ctcatttcct gttgctggca
    12421 agatattccg taaaacttgt ggaataacaa cttttctcat cgtttggcca tgggtcatac
    12481 caatggcttg cgcagcttcg aactgccctt catctactgc aaaaatcccc ccacgaacga
    12541 tctctgtcat gtaagctcca gtattgagag agacaataaa caaagcggca atcgttcggt
    12601 tcaatgaaat gccaaaagcg agtgccagac cgtagaagat gaccattgct tgtaccatca
    12661 tcggtgttcc gcggaatact tcgacataga tagaaagaag tgcatttccg attttttgga
    12721 agaagcgagt cagtgcattt cctgattcag gtaccgaacg gaagacacca atcagtaatc
    12781 caagtgtagt accgacaact gtcccgatca atgcgatgaa caaggtaagt ccagcacctc
    12841 gtaagaacat attgccgtat tgtttccaaa tcgtagtgaa atcagaaatg atgccaggtt
    12901 tttcttcact atcagttgca gcaggctgat ctttgactgc ttggtccata attttggttc
    12961 gttcatcgtt agagatgcca gaaagaatct ggtttacttt ttggatatcc gggtcgcctt
    13021 tacgcatacc aacagaaatc tgaacatctt ccgggtttgt ctggaagcca tctgcaccgc
    13081 tgaattcaag catttccaag tctttgttca cactagttgc cgttacacct tccggacgtt
    13141 cgctgacata accatcgatc attccagaag ctaaagctgt tctcattgct gagaaattat
    13201 ccattgcttg ttgtttctgc acatctggta tctgatcgat cacagtatag tggaatgtat
    13261 ttaactgagc agtaatcttt gctccagaaa gatctgctaa actttttgct tttgcatatt
    13321 tgctatcttt ttgaactact actactaatt gtgattcgta ataaggatcg gtaaaatcaa
    13381 tttcttttcg tcgttcagca gtagggctca ttccggcgat gatcgcgtcg atcttgcctg
    13441 attgaagggc aggagccaga ccatcccatt tcgtttggac gatgactaat tttttcccta
    13501 aaccatctgc tactttttta gcgatttgaa catcataacc gccggcgtaa ctattttggc
    13561 cttgaattgg caaggcatta ttctcatctg tttgttgaga ccagttgaag ggtgcatacc
    13621 cagcctccat acctacgcga aactcatcgc tcggtgtttt ttcatctgca tttgccagag
    13681 atcccacact gaatagtgag aagacaaaag caaatagtac ggcaactgaa accatatttt
    13741 ttttcatttt tttctccctt tctgattata tgatacacga gcggtctaga tgaaatgcgg
    13801 taaagcttta tctgaaaaaa taaaaagcga ccgatttata gatataaaaa ggccgctaaa
    13861 attccataag gtgataaacc tctcacaaaa catagcgcaa cttagcatca ctgctaagac
    13921 agtccgcaag ctcttaactt gcgtcccaac atacactcca agcaaaaaag tgcacatttc
    13981 ggcgatcctc cctattcctg cttctccatg tttgcttcac tccacagggt taatgattct
    14041 cgcgacctct acctcattga ttgaggcatc tcgtattcag ttatttatac aaaaaaagtt
    14101 tacgatactc gagaggcttt gtcaatctat aactgctgtt ttttgaaaaa atactgataa
    14161 atatagaaat ctttactttt accgaaaaaa caatcaaaat gtgaatcatc tattcattgc
    14221 aaacgatagt ataattatac acaaaaatgt atgtaataca ataaaaaaag aactagaaca
    14281 gaaaatttcc tgtgtccagt tccatctcga tcatttaagc tgccgctttc tttggctcac
    14341 gtcctaggat cgcttgcaaa agaacaacga cgccataaat ggctaatatt agataaatca
    14401 aacttgagta ttgggtgatt ccccagcccc atgttcgata tacggctaat gtaatggcta
    14461 aaacaattgc ggcaatcacg ttgaataaag caaaagccca aagatttcca ttttctttgc
    14521 gtacaagata aaaaacagag aaataaacgc ttaacgctaa ccaggcgata caagccagaa
    14581 taacgcccca ataaataaag aatggtaaca tatcgattca cctcactttt atacagcagt
    14641 attgtaacac gttttcattc ttttgtcagt gaacattgtg ataaattgtt caaatgccca
    14701 taacactttt ttattcccat ttttctgact tttcaacaat aaatttgttc gagtatgggc
    14761 tataatggaa agtgaggtga caaaacatga aacaattaac agtagacgaa gcagcaaaag
    14821 agatattagc ttatcagcaa attacatttt tgaccggtgc aggggtatcc acaccttctg
    14881 gagtgccaga ctatcgatct ttgtcaggaa tctaccacgg gatggagcag ccggaatact
    14941 tgctgagtca tcaagcaatg gtgaatgaac cggataaatt ctatcaattt gtcaaaaaga
    15001 tttatcatcc tgaagcaaaa cccaatatca ttcatttgga aatggcacat ctagcgaaag
    15061 aaaaaaatgt ttgggttgtt tcccaaaaca tcgacggttt gcacgcaaag gctaaaagcc
    15121 cgcaactcgt caattttcat ggaagtttgt atcaatgtca ttgtcgaaaa tgtcaacaaa
    15181 cggtagactg gaaagaatat cttcacagcg acaaacatca agcatgcgga ggacaaatac
    15241 gcccagacat cgtgttatat ggtgaagggt ttcaagatgg agtcatggag caggcggctt
    15301 atgccgtcag ccaagcagag ctgatcgtca ttgttggaac gagttttcaa gttcatccgt
    15361 tttgtgatct ggtacagttt cgccagccgt cagccaaaat cttagtaatc aatcaaacgc
    15421 ctgtttattt agcggagcct tatacgttta ttcaagaaga cggagcggtt gttttcaaaa
    15481 aaatacaaga attaggagca gattatgact gaattcaaaa ccgaaaaaga aaaaatgatt
    15541 gcaggcgagc tttattttgc caatgatcct gagctggttg ccgaccgtat gtttgcaaga
    15601 agtcaaagcc aaatcatcaa ccaagcagaa tcagcggaac tgcgatccca attgttgaaa
    15661 gagacttttg gccgtacagg gaaaaaaatc tatatggagc cagttatcaa ttttgattac
    15721 ggatataata tatttgttgg ggagaatttt tatgcaaatt tcaattgtac atttttagat
    15781 gtcagcacga ttgaaatcgg tgataactgc atgttcgctc cgaatgtcca gctttacacg
    15841 gcgacgcatc cactacatcc agtgaaacgt aacagcggtc tggagtacgc aaaacccatt
    15901 aaaatcggta ataatgtttg gttaggcgga ggagttatcg tcacgccggg tgtcactcta
    15961 ggggacaacg tagtagtcgg cgcaggttcg gttgtgacca aatcttttcc cgacaatgta
    16021 gtgattgcag gaaatcctgc tcgtatcatc aaggcagtag aggaagaact aacagaagag
    16081 tctcttgaaa cattacgtca taagatcgat atgatcgatc gagagttggt tgctttatta
    16141 gaaaaaagaa tggatacggt aactaaaata ggacaagtga aaaaagaaga aaaccaagca
    16201 gtttatgatg caaaaagaga gcagcaagta ttggataaag ttgttagtct gctgaaaaat
    16261 aagggatata aggaaacaat aacagatacg tatgttgatt tgatgaaaca ctcgcgcgaa
    16321 taccaaaaca aaatgaagga ggaataagtg tgatcgaagg agagacacgg cgaaaacaaa
    16381 tcatgcaaac cttggcaaat acagaaaaac cagtgagtgc tagcaagttt gcgaaaactt
    16441 ttggagtcag ccgtcagatc attgtaggcg atattgcttt actacgagct gcaggtaaaa
    16501 gtatcgtggc aacagcaaga ggttatatcc tcgaaacaga agaaaccaat actgggcaca
    16561 tcagtaaaat cgctgtccag catggaaaag atcaaacaga agaagaactg agactaatcg
    16621 ttgaaaatgg tggtgaaatc attgatgtaa ttgtggaaca tcccctttac ggtgagttag
    16681 caggaacttt gcacatcaaa acgccacaag atatcgattc ctttatgaga cgttatcaaa
    16741 aaagtaatgc tactttgtta tccgaattaa caggcggcat ccatctgcat acgatccgct
    16801 acccagaaaa gcatatgctg aaaaacatca aacaaaaact agctgaagca ggaatcctct
    16861 acgaaggata acatcgtagc tgctactttt tatcctagaa actattctat catctctgag
    16921 ataaacaccc atatggaaga aataagaaaa aataagaaaa gatgcgtatc caatacacag
    16981 aattttttgg aattgcgatt tctagatgtc tatctaaatt tcgattagtg gagaaacata
    17041 aaaaagggaa gtgcagcttt tgagcactcc ccttttttat aaagctttct tgtagcatag
    17101 tatcgaagga acctgccaat tctctactcg atcaccaata cgccttcttt tagtgcaaat
    17161 ggattttgat cgttgatgtg gtcatagaac atcacaccgt tgatatggtc gatttcatgt
    17221 tgaacgacga tcgattcata attcttcaaa cgaatcttgt gtttttcacc attcatatca
    17281 taataagaca cagtgatttt ggcatggcga acaacatatc ctggtacttc tcgatcaaca
    17341 gataaacagc cttcaccttc ccctaaacaa gcatcttgaa cagaatggct caaaatttta
    17401 ggattataca taacagtact tagagaaggt ccatctgcct ctgggtcagg acttggtacg
    17461 tgaacagcaa tgattctttt agaaatatct aattgaggag cagctaagcc aacaccgcct
    17521 cgtaagtgta gttcttcagc tttgattgga tcttggctgt tttttaagaa ctcaagcatc
    17581 tcttttccta aagagatatc ttcttcgctt aatggaagag aaacttcttt tgcaacttcg
    17641 cgtaaggtcg gatttccttc acgaatgata tcatccattg taatcatgga atatgtaccc
    17701 ctttcttaaa agactgtgtt tcaagtctat tttcgcatta tagtttacca caaaaatgga
    17761 aaagtagtag taagattgtt tgaaaactac cttttcttaa tgattaacta cttgattttc
    17821 aacccggttt caggtaaagt atagctgtgt gctctaggca catgacctgc cacttggtta
    17881 agcgaatcac caacgtttta ctcagtcgca ggggaataat cgaaagaggt gaaagaaatg
    17941 gcaatttcaa aagaacgcaa gaatgaaatc atcaacgaat acgctcgcca tgaaggggat
    18001 actggttctc cagaagtaca aatcgctgta ttaactgaag aaatcaacca cttgaatgaa
    18061 catgcacgcg tgcacaaaaa agaccatcat tcataccgcg gcctaatgaa aaaagttggt
    18121 caccgccgta acttgttagc ttacttacgt aaaactgacg ttcaacgtta ccgcgaattg
    18181 atcaaacgtt taggcttgcg tcgttaattt tagtcaaaaa gagtgggatt cacatgaatc
    18241 tcactttttt tactcgtata tcaattaagt aaaattccgt gatcaagcta gatggactcc
    18301 tactaaccaa atgaaagctg ctcaaaagac ggctttcggc agttttcatt tgtttagtag
    18361 cgtcttgctt tcacgagaag gaaaaggaga agttatgtca gaaaaacaag tattcaaaac
    18421 gacatggggc ggccgtccct tacaaatcga agtcggtcag ctagcaaaac aagcaaacgg
    18481 agccgtttta gtccgttatg gagatacagt agtcttaagt gcagctgtgg cctctaaaga
    18541 agcaaaagat acagactttt tcccattaac gatcaattat gaagaaaaaa tgtatgcagc
    18601 agggaaaatc cctggaggtt tcatcaaacg agaaggccgt ccaagtacgg aagcaacatt
    18661 gactgcccgt ttgatcgacc gtccaattcg cccgatgttt gcagaaggct tccgtaatga
    18721 agtccaagta acgaacatcg tgatgagtgt agaaacagac tgctcgccag ctatggcagc
    18781 aatgctaggt tcttctttag cactatcaat ctcagatatc ccatttgacg gcccaatcgc
    18841 aggcgtagaa gttggtcgtg taaatggcga gtacgtcttg aatccaacag tagaacaagc
    18901 agaacaaaca gatatcgaat tgactgttgc cggaacgaaa caagccatca atatggttga
    18961 aagcggcgcg aaagaagttt cagaagaaga catgcttggt gcattgctgt ttggttttga
    19021 cgctatcaaa gagttagttg ctttccaaga agaaatcgtt caagcagtag ggaaagaaaa
    19081 aatggaagtg actttactgc aagttgatga agtattgaaa aaagaaatct ttgatgcttc
    19141 ttacgctaca atgaaagcag cagtaatgac tgaagaaaaa cttgctcgcg aagataatat
    19201 cgagcaggtg aagatcgata tccgcgaagc ttatgctgag aaatttgcag gtcatgaaga
    19261 tgaagatcac ttgttgaaag aagttaaaca aatcacagaa gatcttgaaa aagatgtcgt
    19321 acgtgaattg atcaccatcg ataagatccg tcctgacggg cgtaaattgg atgaaatccg
    19381 tccattgtca tcagaggtca gcttattgcc acgtgtacat ggatcaggtt tgttcacacg
    19441 aggacaaaca caagctttat cagcttgtac actcgcacct ctaggagagc accaaatcat
    19501 tgatggttta ggggtggaag tcagcaaacg attcatccac cactacaatt tccctcaatt
    19561 ttctgttggt tctacaggac gtgcaggctc tccagggcgt cgggaaatcg gacatggcgc
    19621 attaggtgaa cgtgcgttag cacaagtaat cccttcagaa gaagaatttc cttatacgat
    19681 ccgtttagta gcagaagtat tggaatcaaa tggttcttct tcccaagcaa gtatctgtgc
    19741 tggcacatta gcattgatgg atgcaggtgt accaatcaaa gcgccagtcg ccggtattgc
    19801 gatgggactt gtttctgatg gtgaaaacta tacgatccta actgatatcc aagggctgga
    19861 agatcatctt ggcgatatgg actttaaagt tgccggtaca aaagatggta tcactgcttt
    19921 acagatggat atcaagattc aaggaatcac agaacaaatc ctaactgaag cattaactca
    19981 agcaaaacaa gcacgtatgg aaatcttaga agaattgact tcaacgattg ctgctccacg
    20041 tgaagaatta agccaatatg caccaaaaat cgaaatgatc cagatcgaac ctgctaaaat
    20101 caaagatgtt atcggtaaag gcggcgatac gatcaacgga atcatcgatg aaactggtgt
    20161 gaaaatcgat atcgatcaag atggaaaagt aagtattgct tcagctgatg cagaaatgat
    20221 caaaaaagct atcaagatca tcgaagattt gaccaaagaa gtcaaagttg gtgaagttta
    20281 tctagggaaa gttgtccgta tcgaaaaatt cggtgcattc gtcaacttga tcaaaggaaa
    20341 agatggactt gtccatattt cccaattagc aaacgatcgt gtcaataaag tcgaagatgt
    20401 tgttaaacta ggtgatgaga tccttgtgaa agtaactgaa atcgataaac aaggacgcgt
    20461 aaatctgtca agaaaagcaa tgttgaacga agatggttca acgaaaaatg aagaaaaata
    20521 atttaattca taggaataat gtacttgtga tcgtgaaggt gacaggggga caataattat
    20581 tcgaaaaaag gggactgtga caaaagccgt gtcacagtcc ttttttttcg aataaacggt
    20641 gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt taaaaacatt aggagctgtt
    20701 tttacttggc tgttcttatt acttgatgca gaacagcttt tttacaacct ctccaataaa
    20761 cgatgttcaa tcagctgtcc ctcgatatta gctcaaaaaa cggcaaaata tgaggaacta
    20821 tttttccgtt tgtttctcta atactcaggt caaaccgctg atttcacaac ctctgattca
    20881 cggtgttcca gaagcagctt ctacggaaat aagctgaaca cttttgttac aacttctttt
    20941 tttctggagt ttcttggagc atatttttgg agtccttctg aataaagcgt aaaataaaag
    21001 tgagaaatat ggataaagga gggaaaggag atgctgaatt ttttatggtc gttatttatc
    21061 ggcggtatat taggtttgat agctggcgca attttaggaa aggaagtccc gggaggcgta
    21121 tctggaaata tcattatcgg ttttttaggt agttggctcg gcgaattttt actaggacca
    21181 ctgggaccgg aactcggcgg tttttatttg ataccagcat tattgggtgc aattttttgc
    21241 cttgctcttt attcatttgt ggcaagacgt atcagagaac gtccaaagaa agaataattt
    21301 tattcaaata agttgacact tcagtgtgtt acttggataa ttagaagcaa tttaggaaag
    21361 cgggtgtcat ggataagaat atcatctatc attcaagctt acctaaagga tatccatata
    21421 acaagaaatg agggaaaaac gtggcaacaa cgcacaatac caaaaagata tcaatggctg
    21481 gcctgcttgt agcgatggga gtcgtctacg gagacatcgg gacaagtccg ctttacgtta
    21541 tgaaatcgat tgttgaagat aatggcggat tgtcaggaat caatcctgat tttgttatcg
    21601 gatcagtctc attgattttt tggacattga ctctactgac aacaatcaaa tatgtcgtga
    21661 ttgcattgaa tgcagataac catggtgaag gtggaatttt ctcactctac acacttgtcc
    21721 gaaaaggtgg gaaatatttg atcattcctg caatgatcgg aggagctgcc ttactagcag
    21781 atggggtatt gacccctgct gtgacggtaa cgactgcgat tgaaggcttg cgaggaattc
    21841 cgcagttttt tgaccggttc ggtaataacc agaacgtgat cgtcattatc actttagtta
    21901 ttatttttgt tttattcatg gtccaacgct ttggtacaga tattgtcgga aaagcattcg
    21961 gaccaatcat gttcgcttgg tttacttttt taggcgtaat gggattaatg aacttcagtc
    22021 aagactggac agttatccgt gcactgaatc cttattatgc tatccagctg ctttttagtg
    22081 cagataacaa attaggcttg ttcattcttg gaaatatttt cctggctacg acaggtgcag
    22141 aggcgcttta ttccgatctt ggacatgtcg gcaaacgaaa tattcgtctg agctggcctt
    22201 acgtcaaaat ttgtttggtt atcaattatt ttgggcaggc tgcgtggttg ttgaatgttt
    22261 atcaaaaccc tgaagcacag caaatcaaga acctgaatcc attcttccaa atgatgcctc
    22321 aaagttggat cgtgatcggc gttgcttttg caacagtcgc tgcagtcatt gcttcgcaag
    22381 cattgattac aggttcgttt acgctagttt cagaagcaat caagttgaaa ctgctgcctc
    22441 ggttaaagat catgtatcct gggaacagta tcgggcaaat gtatatccca gcagtcaatc
    22501 tgattttatg gatcgcctgt tctctagtgg ttgtcacatt ccgaacgtct catcatatgg
    22561 aagctgctta cggtctatcg atcactgtga cgatgttgat gacaacagca ctgctttact
    22621 tttatcttat ccaaaatggg cattctcgtt ggcttgcata tttagttacc tttttcttcg
    22681 gtgcgataga agtcgttttc tttatctcaa gtgttgtcaa attcttccga ggcggttttg
    22741 tagccgttct gattggtttg atcatcttag tcgtgatgtt tatttgggaa caagggaaca
    22801 ttattcgcga atcgtcaacc gaggatgtgt ctttgagaga ttatatcccg cagcttagag
    22861 aattacgtga tgatacttct ttaccaatct atcagacaaa tgtggtcttc ttggttcctg
    22921 atatggctga agataaagtt ggccgacagt tcatctattc gattttagat aaacggccta
    22981 aaagagcacg agtctattgg tttgttcatg tggaagtgac ggatgagcca tatacgaaag
    23041 aatatcaagt agatatgatg gacactgatt ttattgtcca agtgaatctg ttcttaggct
    23101 tcagggtcca acaggaaatc aacgtatatg ttcgccaagt catccatgat ttgatgaaac
    23161 aaggccgttt gccaaaacag ccacaaagat actctctgac accagggcga gaagttggcg
    23221 atttccaatt tgtcctgatt gatgaagtag tttccaatgt gacaactcta ggaaaatggc
    23281 aacgtcaaat catgcaggca aaactggcaa tcaaaaaaat cgctacctcg ccagaaacat
    23341 ggtttgggct agagtacagc gaagtaaaac atgaaactgt tccgcttatc attggaaata
    23401 ctcggaaaac atggttgaaa gagcggttgt gaaagtagcg ctttgacctg aactaatgtc
    23461 gaatgtcagc tacttgaaca ccgtttatca gaggtcatga aaaagctgta ctgcaacaaa
    23521 cagtaagaac gagcagataa aaacagcttc tcatgttttt catctgtttg ggcttaatgc
    23581 cgcagatgca agcttttgaa taccgttttt atcagaggtc atgaaaaagc tgtttagcaa
    23641 caagtaacgc aatgattttg aagtgataag gagatgtggc tgaaaaatgc cgcatctcct
    23701 tttttattcc taaatatgaa tacgcattat tttcaatctt tttgtagaaa aagggtacaa
    23761 tagaaaaaga gaaaggcggg agaatatggt tatcaatttc attattttat ttttgatgat
    23821 tgcaattact tcattttttg tagcaagtga atttgcttta gtgaaaatca gacgatcaag
    23881 attggaacaa ctagaaaagg aaaatgtaaa gaacgccaag ctagctcttc atgttacaca
    23941 ccatttagat aactatttat cagctagtca attaggaatt acgctaaccg gtttgatcat
    24001 cgggtgggtc ggtgaaggat ctgtcgcggc tttactagaa ccagtgatcg gtaaactacc
    24061 ttttagttcg gctattagca gtacaatctc tgtggcgctg ggatttattt tagttacgta
    24121 tatcgatgtg gttattggcg aactgcttcc taagagctat agtatcgtca atacggagaa
    24181 ggtcgtcttg ttcgtagtta aaccacttca ttatttttat aaagtaatgt ttccttttat
    24241 ttgggtattg aatcattcag ctgcagggct agggaagcta ttaggtgtgc gtctcgtatc
    24301 tgaaggagaa gagacattgt ctcaagaaga gctgacatta gtcgcattga attcttatga
    24361 aaaaggagag ttgaccaaag aagaatatca ttatttggaa aacgtgtttg aatttgatga
    24421 taccttggca aaagatatac aagtagaccg tacgtccatg caagtattcg aagcttcaga
    24481 aaccgtcaaa gaagcgataa aagcttcgat tgaacaagga catactcgct atccggttat
    24541 cttagaatca aaagacaatg ttttaggtta tgtcaccttg ccagatttga tcaagcaatc
    24601 ttataaagat gaccagctga cagtagaaca gttgattgaa gagccgatcg taacaactga
    24661 aacgatccct ataaaaaaat tattgacgat catgcgaaaa aagggaaagc atattgctat
    24721 cttaaaagat gaatacggag ggacaagcgg cttagtgacg attgaagata ttttagaaga
    24781 aatcgtcggg gaaatccgag atgagacaga tttggacgaa gcattgatag cggaacagtc
    24841 agatggctct tatattatct ccggcaaact aacattagat gatttccagc gttattttca
    24901 tgtagagatt ccagagtttg aagaaacgaa ttttactaca cttgcgggct ttgcttctag
    24961 ccgatataaa gaaataaaag caggaacaat cattgagatt gcatcattcc gtttcacggt
    25021 attagaatac cagcatgcac atatcgatta tttcaaagta gagtccacgg aaagaaaaac
    25081 agaataaaag aaaaaaggct gtgacatttt ttgtcacagc ctttttccta cgattagtca
    25141 agtaaaaggt gcaagaaaaa tagctcgtaa acgaaagcga caaaatcaaa gcacaccaaa
    25201 taaagccccg ttcgtaaaaa attttcgatc tgggcaaaga agtggtatac tgtaagtggt
    25261 ttagttaatg gtccactgag accgcgtacg catagtcgta ctctgtatgt gccctaacca
    25321 tagcgtgcat acatttcaga agcttgttca tacaagcaac tgtggcaacc ttatcttt
//
LOCUS       DACEQH010000040        25325 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.040, whole genome shotgun sequence.
ACCESSION   DACEQH010000040 DACEQH010000000
VERSION     DACEQH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.040"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(324..893)
                     /locus_tag="IW833_001731"
     CDS             complement(324..893)
                     /locus_tag="IW833_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1307852.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="IW833_001732"
     CDS             complement(967..2256)
                     /locus_tag="IW833_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307853.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="IW833_001733"
     CDS             2610..3458
                     /locus_tag="IW833_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ1307854.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="IW833_001734"
     CDS             3472..4545
                     /locus_tag="IW833_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ1307855.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6693
                     /locus_tag="IW833_001735"
                     /pseudo
     CDS             4535..6693
                     /locus_tag="IW833_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(6805..8976)
                     /gene="copB"
                     /locus_tag="IW833_001736"
     CDS             complement(6805..8976)
                     /gene="copB"
                     /locus_tag="IW833_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ1307856.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(8996..11182)
                     /locus_tag="IW833_001737"
     CDS             complement(8996..11182)
                     /locus_tag="IW833_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ1307857.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11182..11391)
                     /gene="copZ"
                     /locus_tag="IW833_001738"
     CDS             complement(11182..11391)
                     /gene="copZ"
                     /locus_tag="IW833_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ1307858.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11404..11844)
                     /locus_tag="IW833_001739"
     CDS             complement(11404..11844)
                     /locus_tag="IW833_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ1307859.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11918..12391)
                     /locus_tag="IW833_001740"
     CDS             complement(11918..12391)
                     /locus_tag="IW833_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HAQ1307860.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            complement(12701..14167)
                     /locus_tag="IW833_001741"
     CDS             complement(12701..14167)
                     /locus_tag="IW833_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1307861.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(14478..16562)
                     /gene="cadA"
                     /locus_tag="IW833_001742"
     CDS             complement(14478..16562)
                     /gene="cadA"
                     /locus_tag="IW833_001742"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1307862.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            complement(16704..16892)
                     /locus_tag="IW833_001743"
                     /pseudo
     CDS             complement(16704..16892)
                     /locus_tag="IW833_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(17086..17445)
                     /locus_tag="IW833_001744"
     CDS             complement(17086..17445)
                     /locus_tag="IW833_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ1307863.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(17475..17816)
                     /locus_tag="IW833_001745"
     CDS             complement(17475..17816)
                     /locus_tag="IW833_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307864.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(17813..18487)
                     /locus_tag="IW833_001746"
     CDS             complement(17813..18487)
                     /locus_tag="IW833_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1307865.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            complement(19282..20580)
                     /gene="asnS"
                     /locus_tag="IW833_001747"
     CDS             complement(19282..20580)
                     /gene="asnS"
                     /locus_tag="IW833_001747"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ1307866.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(20620..21810)
                     /locus_tag="IW833_001748"
     CDS             complement(20620..21810)
                     /locus_tag="IW833_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1307867.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(21831..22358)
                     /locus_tag="IW833_001749"
     CDS             complement(21831..22358)
                     /locus_tag="IW833_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1307868.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(22405..25194)
                     /locus_tag="IW833_001750"
     CDS             complement(22405..25194)
                     /locus_tag="IW833_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1307869.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDSSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAIYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKEIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
BASE COUNT         7096 a         5392 c         4458 g         8379 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcatgaagca actttaattc gtaaagtcga agctgtcgaa
     5821 cagtatttaa aaacaagcgg aaatttccgc tattccaaaa cggataccgg acatactccg
     5881 aaagaacacg attatgtgga ttatttcctc tttgattcaa aagtcggcta ttgtgacaac
     5941 ttttcaactt ctatggtcgt ccttcttcga acacttggta tcccagctcg ctggacaaaa
     6001 ggttttacaa atgggctcag aacaggcaca gataatcaag ggaatgagga atacagcatt
     6061 ctcaatagtc atgctcattc ttggccagag gtatactttc ctggatttgg ctggcttcca
     6121 tttgaaccaa ccccttcatt tgccaatcca atgggttcac aaccagttgc tgaaacaacc
     6181 gaaaccgtcc agcctgctat cgaacaacca aatgaaagcg aaaatatggc agaaactatt
     6241 tccagtgaga gatctgaggt cattccatca gaagaaacaa cactcacacc agcttataaa
     6301 atcgtcttat tcagccttgg ggtgtgttta tacgtcttgt tgttctttct attgttattc
     6361 agaaaatctc tgcgctggac aagttatctt atctatcttc atttttttgc ttctactaat
     6421 acgattgcat atcaaaaact tcttaaccat ttggaaacat tggtaccgcg caaacccgca
     6481 gaatctttgc aggaatatag cctgcgaata gaacaagaat tcaagctaga tctgcgtttc
     6541 attcgattaa ctactcaata tgaagcagaa atatatggac gacaagcaga tccggtaaat
     6601 ttcgataaaa aaacctattt agaaattacg catataataa gaaaaacggt atacaaaaaa
     6661 gttctgacaa aagggagccg cttcaaaaaa taagataaaa tttctggaga atctacttct
     6721 ggaatgccga gaaccagctg cttgagtatc gattattcga aaaaaaggtt gtgacacgtt
     6781 ttttgtcaca accttttttc attattattt gattttcaat gtcaaagcat ttagagctac
     6841 aactaccgta cttaacgaca tcaagacagc accaactgct ggacttaata tgataccaat
     6901 tggcgcaaga attccggctg ctagcggaat tgcaagaata ttgtaacctg ccccccacca
     6961 gaggttctgg atcattttct ttcgcgtttg cttggctaga tcaagaaaat gcaaaatatc
     7021 ttttggatcg ctgtttgtca atacgacatc cgcagaatcg attgcgatat ctgtccctgc
     7081 accgattgcc atcccgattg aagcacgggc taaacttggc gcatcattga tcccatcacc
     7141 caccatgatt acttttttcc cttggtcaag gtatttctgg acaacagctt ctttatcatc
     7201 tggtaataaa ccgccataat attcttcgat ccctaaataa tctgctactg cttgggcagc
     7261 gtttttattg tcaccagtaa gcatgacagg agtgattttt ctttctttca atgttcggat
     7321 aaattctttt gcttctgttt ttactacgtc acctaaagca acgattgcag ctagatgatt
     7381 ttctaaaatc aggaagctga tggtatttcc ttgttcttgg taaggtttca ataatgcttg
     7441 ttctacgttt aatccgagac gctcaacttc tttttcatta actattttca cttgttggtt
     7501 tttgactgtt gcctctagcc caactcctga aagatttttt agattttcgg cttgataagg
     7561 cttgatttca tgttctttca aataattcat aataccaaca gccaatggat gattggcatt
     7621 cgcttccaat gctcctaaat attgaaggat ttcattttta ttgaacgcat catcaagtac
     7681 ttcgacaccc gttacagtaa attttccttc tgttagtgtt cctgttttgt ccagcatgat
     7741 cacatctaga tcattggctt gctccagtgc attgcggttt ttcaaaagta atccgttttt
     7801 agctgctatt gatgtagagc gagcaacaac taatgggata gctaaaccta gagcatgagg
     7861 acaggcgatg ataaagactg ttaccatacg ttctaatgca ttaggaagat ctgtcaagaa
     7921 caaccaggcg atgaatgcca ggatacccac aattaatgcg acataaaaca gccattttgc
     7981 cactttgtca gataatgact ctagcttgga tttttctcct tgtgcttttc gcaccatttc
     8041 cataaccttc gctagataac cattttcacc agttccagtt acttcgattt cgatcgttcc
     8101 atccccattg atagaaccgc caatcacttt gtcattggct tgttttttga ttcctcttga
     8161 ttcgccagtc acagctgatt cgtcaacaat tgtttcacct ttcaagatta caccatctgt
     8221 tggcatttta tcaccagacc ggacgatcaa acgatcacct tcgtttactt ctttcaatga
     8281 aactgtctct tcttttccat ctttcgtcaa gcgttttact gattctggca gtaattctgc
     8341 tagtttttgt aaagcattgc tggcattcga cacagcattc atttcaatcc agtgacctag
     8401 tagcataata acgatcaaag tagccaattc ccagaagaaa tccatgacgt gtgtatgtgg
     8461 attgagcaga tttgcgataa acgaatagac actataaata tatgcaactg taatccccat
     8521 cgcgatcagt gtcatcatag caggactttt ttgtttcaat tccatcttag ctccacttaa
     8581 aaatggctga ccgccataga taaatagtat agttgctaag atcaatacga gccaatcaga
     8641 accaggaaac gttacttgaa atggaaattc cattcccatc attggtgaaa aaagaatgat
     8701 tgggatagct aggataagcg atagccaaaa tttttgtttg aaatttccca tgtgcatgga
     8761 atgatccatg ccgccatgat cgtgatccat gtgcatcgaa tggtccattt gtttgctgtt
     8821 tgctatatct ccatggttat gtcccatgtg catggaatgg tccatctggc tatggtcatg
     8881 tttcatctcc atctgttttt catgagattc tgtactttct ttgtttacta cttctttttt
     8941 atgttcatgt tttttctcca tatcgttccc attcattttt actccatccc ttctcttact
     9001 ttttcatgtg ccgatttaag ctcagggagt tcaacaatac actgacggaa ctaaacgcca
     9061 tcgctcctcc tgcaatgatt ggattcaaaa agcctagtgc ggcaaaagga ataccaatcg
     9121 tattatagac aaaagcccag aaaagatttt gtttgatttt tctcaatgtc accctagaca
     9181 actcgattgt ttgtgcaatg gatgccaaac tgctactcat cagcgtcaca tcggctgttt
     9241 ccatcgcgat atccgttcca cttcccattg ccataccaac atcagctaat gctaaagccg
     9301 gtgcatcatt gattccatcg cctgccatac cgactttttt gccatctttc tgaagttttt
     9361 ctacgtaggc tgctttatcc tctggaagta cttcagcaaa gatatgcttt ggatcaatac
     9421 caacttgttt accaatcgtt tcggctgcca gtttgttgtc tcctgtcagc ataaaaacat
     9481 ctaatccttt attctggagc tgcttgattg cttgtttggc ttctagtttg atttgatcag
     9541 ccacggcaat caacccgatc acttgctttt catcagctaa aaacatgact gtttttccct
     9601 gctgttctaa ttctaacgcg tgttcttggt attcatcaaa tgataggttc aattcaatta
     9661 atcgtttacg tgtacccgca aaatagcgta ctccgttaat cgtaccgctg attccagcac
     9721 ctgggtgagc tgtaaaatca atcatatcgt atgttgctgc ttcttgcagt tttccgtatt
     9781 ccacgattgc tttacctagt ggatgttcag aagcttgttc taaagtataa aaaatagata
     9841 ggacttcttt agaaccgact agatctgtaa ctttgggctt tccttctgta atcgtcccag
     9901 ttttatccaa aacaatgctg tctaagtgag cagctgcttc taatgcttct ccgcctttaa
     9961 ttaaaattcc atttcgtgca ccaacacccg ttcctaccat aatagctgtt ggagtcgcta
    10021 aacctaatgc acatggacac gcaatgacta atacagagac actatggatc aatgcgagtt
    10081 gccaatcacc agtgatcaaa cctgtagcta tgagcgtgat aaacgcgatg actaatacga
    10141 tcggaacaaa aatccctgag atccgatctg cgattttttg gattggggct ttgctccctt
    10201 gagcatcttc caccatttga atgatttgcg ctaatactgt ctgcttgccg acttgtgtga
    10261 cttccatgtg tagcagacca ttcgtattga ttgtcccgcc aaaaagcgtg tcatccggat
    10321 tcttttctac aggaagattt tcaccagtaa gcatactttc atctatcgca ctgcttcctg
    10381 aaataattcg accatctgct ggaatctgct caccaggacg gatgactagt tgatcaccaa
    10441 cgactacttc ttcaatcgct aacgtttctt ctttcccgtt tcttatcact tgggcggttt
    10501 ttgtttgaag agacatcaat tgtttgattg cattacctgt tttcgttttt gctgtatgtt
    10561 ccaaatattt gccgagcaaa atcagcgtga tgatcatact gctgctttca aaataaagat
    10621 cttgggggtg tccacggaaa aatccgttgt aaatgctcaa tgcaaaggca gccgatgttc
    10681 caattgctac caatacatcc atatttggtg ctttcgactt gattgcatgg tacgcacctt
    10741 tataaaaacg agcaccgaca taaaattgga caggagcaga cagcactaac tggacgattg
    10801 gaaaatgaaa aaagtgaaca atcgccgcat ggcttcctaa catcatagca atcattgaca
    10861 acatcaacgg caaagtcaaa accgtactca atataagatc gaatagcatt tttttcaaat
    10921 acgcttgctt ttcttctgca attttttgtt tgtgggcttc atcgtacaaa attgcaccat
    10981 agccgatgtt ttctacgctt tggattaatt tttcagtggt cgtcccttca tatttgacac
    11041 ttgctttttc agtggccaag ttgactgtgg cattgaccac accaggttgt ccgttcagct
    11101 ctttttcgat cctagcagaa caatttgcac aagtcatacc tgtaatgacg aacgtatcca
    11161 ttttggtttc tgctgttgcc atcaaatcac ctctgcttta taacccaatc cattgattgc
    11221 ttgacaaatc ttttcggatt gaacttttgt ttcatcaaac ttgaccgtac cgtttgcttt
    11281 tttcaaattt actttcactt tttgcacgcc gtcaagtgcg cttactgttt cttctactct
    11341 tgctgcacaa tgattgcagc tcataccttc gattgaaaat tgttgtttca ttttttcttc
    11401 ctcctattga tgttgtttac attcacattg acctgggata cagttacatt cgatcgtatc
    11461 tactggttgc ttttgttcca gttgtttcaa aatctcattg atatcttctt gagtcaaagt
    11521 ggcttcttct actagatccg caatcgtctc tccaatcctt tttgcacaga tatgggaaaa
    11581 aagattttcc gtcgcactgc gaactgtttc catctccgag acagctggat gataaatgaa
    11641 tttttttcct tcttgttctg tccagatcac ttcttttttt accaaacgac ccagcaaagt
    11701 tttgatcgtt gctggtttcc aatccatcga atcagctaag atctgcgtga tttcttgggc
    11761 tgtcgtctgt ccaagtgtcc aaataacacg catgatctcc cactctgaat cactaatctt
    11821 tactggcgtt ttgacttttt ccatttattt cacacctttc gtttacaaat gtaatcatta
    11881 acttatgaat acagattaca cttgtaaaca aaaactgtca attatttttg ctctctattt
    11941 tcaatttagt taaaaattct tgtttctctc gtcttgtttt aaggatgatc agtttatttc
    12001 ttgcattagc tacttggatc agctgctgta tttttggttc attgtttcct tcaaaatgcc
    12061 agataaaaga taaaaattct aagtatttcc tgtttagttt ctccgaaaat gattcaggca
    12121 tatctggccg agattttttg tttatctttc tttttagaga acgccaaact actcggtaga
    12181 tggcgagata acgcggcact ttaagccaaa tgatcttatc tgcttcttta atcctttcct
    12241 ctaacgtaga agtataattt ccttcaacga tccatgacgg attagatacc ataaacgatt
    12301 gctgttcttg caaaaaccac ttttttgcct gaatgcggaa agccggtgat ttctttgatc
    12361 ttccttgcga gggacgattt tcccgaccca ctttgtccaa tcacgatgat cttcattctt
    12421 ctccctcact tctctttgga atttggttcg atcataccac ttataacgcc gcaaaaaaac
    12481 aagggactgt gacaaaagtc gtgtcaaagt cccttgtttc gaataaatgg tgttcaatca
    12541 gctgaccttc agcattagct caaaaaacgg caaaatatga ggagctattt ttgtttggct
    12601 gttcttatta cttgatgcag gacagctttt tcacaacctc tgattcacgg tgttccagaa
    12661 gtagcttctg tcacaacctc ttgtaacaag tatttctatt ttaaggctga accactgttt
    12721 ctggttcagt aatttctttg tttttgtctc gacgagcaat tgctggcaag atcataccta
    12781 ataagatcaa gacaattggt gtgatgatat tcgtgaataa ttggaaccac catgctttag
    12841 gatcagcagt ataatctact tttggcacca ttcctaggat acatgcaaac gcagtgaagg
    12901 caaagcacca agcaccaaag ataaaaccga tttttggatt tttaacaaat ttatattcag
    12961 aattgaattt cttataagct ttattcaaca tcatatatgc caagaacacc cataggtaac
    13021 gcattggcat gacaactgag ttcaagttcg tcaaccattt tactaattca ttcatatctt
    13081 tgataccaaa gatcggcaac aaaataatga ttcctactag tatacctgtc agaagataac
    13141 cattcaccaa tgtaccttta cgattacgtt tacgtagcca gtttggtaca aattctggat
    13201 ccgcatctgc taacaagatt tttagtggtg catcaattga aaaggctaat gcggagattt
    13261 gtccaatcat gtttgtcaat gcgtaaatga tcatcaatga gttaccgata ccatagtaat
    13321 tacctaaaat ttgaaaagca ctgtatgccc catttgccat caagtcttta gggatattat
    13381 cgctagcaaa cagcataccc attgcgactg agcctaatac agcacaaatc cctaccattg
    13441 ctgctaagaa gatcatccct ttaggaaatt cctttgcagg atttcttgta gagttgacat
    13501 aaggggatat tttttccgcc cctccaactg caaatactag catcgaaacg gttgtgaaat
    13561 aactgaaatc aaatgttgga atatatgttt tcacttgatt catatgtggt gtggcaaatt
    13621 ccatcgttgg attgatgaac ggtgccgtta cagctaaaag aataaataag atcgacatga
    13681 cgaacatcgc tgttccggcc agtccgccaa tcactttcaa agtagaaagt ccttttgttg
    13741 ataaatataa aaaggcaaga aatataataa gtgaaataac tgctaccatt tgtactgaca
    13801 tcgtatttac gatatttcca tttccttgga ctgcccaacc accagcaatc agaattgctt
    13861 gaggcttttg tgccaaataa ggaatatgga cgacccagta agtccaagcg gcatagtatg
    13921 ccagtcgttt attagatgtc gtattttcga cccatgaact gacgcctcct ttactatcct
    13981 tgaaggctga tcctaattgt ccaacgatca atgcgtatgg aataaaataa atggccaaaa
    14041 tcaatagcca agaggtgaca actgatactc cttgctgagc atagttatta actacattcc
    14101 ccatgcccca aaccatgtta aatgcaatta acgcaacagt aaaccagcgt aattgtttgt
    14161 tgttcattcg ttctctcctt tatgataaaa atttgttatc cagatgtaat catattcaat
    14221 ttttaacaaa cttacaagac agtgttattt tatgtgtgta ttcttttatt taatacacat
    14281 cgttttttca tattttttcc atcaaaaaaa taagttattt tcttttattt cattgacctt
    14341 attgaatttc aagctatttt gaattttctg caaaattaaa aacattagtt attttataat
    14401 cctatctatt ttctttacat ttttcattaa gaaaaggata taaaaaaaag aggacgttca
    14461 ttcgtcctct tcattgatca acgtttattt tttacatgca agcttcgcat ggcattcaaa
    14521 acagctaata ctgtcacccc aacatctgca aatactgcat ctcccattga tgcaaaacca
    14581 aaagcaccta aggaaagtac caaaattttt atcccaatag caaagacaat gttctgtttg
    14641 acgattttca atgttttctt tgctaaatgg attgcttctg caatcttcga tggttgatca
    14701 ttcatgatga ctacatctgc tgcttcaatt gcagcatcgc ttcccaatcc acccattgcg
    14761 attcctacat ctgctcttgc tagcaccggt gcatcgttga ttccatctcc gacaaatgcg
    14821 gtctttttat tgttactttg taagatttcc tctaaccgct gcactttatc ttctggaagc
    14881 aattcgctat atatcttgtc cacgcctact tgcttcccga tatgatcagc aatttttttc
    14941 gagtcgcccg tcaacatgac tgtgtttttc acaccagcct gttttaattg aaccaatgcg
    15001 tctacggcat cttctttcaa ggtatctgca ataacaagat aaccactgta actttgatcg
    15061 atcgctagat aaagcaatgt accgatttct tgacttgaag gaaaagagat ttcaaatctt
    15121 tccatcaatt tgtgattacc tactaatact tgtttgccat caacttcagc taagatccct
    15181 tcacctgcaa attcctggat attagctgtt ggtaaaagcg gacctgcgta gccttctaaa
    15241 acagattgag caatcggatg tgtagaaaac tgttctgcac ttgcaacata ttgcataaat
    15301 ttctttggat caattcctgt tggatcgatc gtctggatcg aaaagtcacc tttcgttaat
    15361 gtgccagttt tatcgaatac cactgtctca gtttgtgcaa gcaactccaa gtaatttgaa
    15421 ccttttacta atacaccgat tttacttgct cctccgatac cgccaaagaa actaagtggc
    15481 acggaaatca ctaatgcaca tggacatgag atcactaaaa atgtcaatgc tcgatagatc
    15541 cattcagaaa acgccgctct agaaacaaca agtggaggta aaacagctaa taaaacggcc
    15601 aatcctacaa cgactggtgt gtaatagcgc gcgaatttag taatgaaatt ttcagctggt
    15661 gcttttttac tgctggcatt ttctactaat tctaataatt tgctgatagt agaattttca
    15721 taagattttt ctacttgaac taaaaatggt ttatttttat tgataaatcc gcttaatact
    15781 gtttcaccaa catatacaga tttagggact gattctcctg taagtgctga cgtatcgact
    15841 aatgattctc cttcgatgat cgttccatct aacggtacac gttcaccagg cttcacaagt
    15901 acaaactgac cgatcagcac gtcttcaggg ttcacggtca tcaatccttc cgccgtctgc
    15961 acgtgagctg tttctggacg gatcgccatc aattcgcgaa tcgattttct tgaatgactg
    16021 actgccaatc cttggaaata ctcgccgatt tgataaaata gcatcactgc tacggcttct
    16081 ggatattcac cgatcaacat agcgccaatc gtagcaacag acataagaaa attctcatca
    16141 aagatttttc ctttgacgat attcatcact gccttcttca ctacatcata tcctatcagc
    16201 agataaatca ataaataccc tatcaagcga attggttcat taaaatcaaa taatagcaat
    16261 aaagcgatac ctgcaaggga aaataaaatc cggaacagcg tatttctctt tttttcattt
    16321 ccatgttcag atactttttc cttatctatt tctgttactt tgacatctgg ttctaattcc
    16381 ttcactactt tttttgtttc ttgttcaatc gtagccaaat gatcttcctc tgcgtcaatc
    16441 gttagttttc cagaagtgaa gtttaccgtt gcttccttga ctccattgat tttctgaact
    16501 cctttttcga ttttcattgc acagttcgca caatctaaac cttctaatct gtaacttttc
    16561 attttaatga cctcctcgcc tcaacatatg aatatctctt cataactaat tataaatgaa
    16621 tggttattca tatgtcaagg tatccgaaag aaaaaatgaa aaacatctgc atcggaaaat
    16681 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgccactgct
    16741 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
    16801 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
    16861 cctgcaaaat gtccttccca gatatacgaa gctccactac gctttcaaaa atatagaaag
    16921 agttctttaa tgaaaattat ttcaactttc cataaacagt aaccccatat aaataaaaga
    16981 cgtaaaagtc acatagaaga acttatacag gaaaacgcat agagtcaggt gaaatacatg
    17041 tatcccacct ggctctatgc tttatctaac ctttattatt ctcttttata caatttctgc
    17101 accgaatggc atcaacgcca atcgagcaag tttgaaagat tgcttggcaa aaggtatccc
    17161 tatgatcgtg atacatagaa ggatgccgct gacgacatgt gcgagagcaa ttggtaggcc
    17221 tgaaacgatc agccagatga tattaacgat caatgacatg ccagactggt gataaattac
    17281 ttctttgcca aaaggcgcaa aagaaaggga ggcgatctta aaacattgca taccgattgg
    17341 gatacctatg atcgtgatac accaaagtat ccccgctgct aaccagctaa gaccaccgat
    17401 tagtccgccg aatataaacc agatgacatt tcctaaacaa ctcattgtat ttctccttct
    17461 attactgttt ttgattaaaa ctgggcaaaa attgatttta ttcttctttt tttagcttcc
    17521 agcatcaagt attctgcaaa ttttttttgg atttcttccg tattttcttt tgttactaat
    17581 gtgtgttgga ttagttttcc tgatggtcgc tgatccaatc gttcttctga ctgttgcacg
    17641 tccgcttgcg caaatccgat aatccgtttt tcttttgtta cagcaacgag caatagttta
    17701 ttcttatcat ttaggtcagc tgccgttttt gcttttacga atgattctgg atgatcagta
    17761 atcagctgat tgatttgagg taaatcttct tttactgccc accttatttc catcattttt
    17821 cacttttctt tcgctcattt ttatctccac taaaaaaatc gactgcgaca attgcatcta
    17881 tccagctttg tgcaatgatc tcatgtccgc caatcgttgg gtggacccca tcttctcttg
    17941 tatatttcga aaaaccataa gcaatcccta aagcattgaa tataccatct aaaggaacca
    18001 gcaatgcgcc atattcctta gcaagtttac gaacaatttg gattttaggg tctaagtctt
    18061 tacgccagcc atgacggtcg tcggttatag gaagtacaaa cggctccatc aagatgatgt
    18121 tgcagatttc cgctgtttga agttcattta gtaattttcg ataaacggat tcaaattgct
    18181 gagcagattt ctcagttccg aatatagcct gatcgctagt attatgccag gtatcattga
    18241 ttccgatcaa aatcgataca tagtcaggtt gtaaagctaa acaatcttct tgccagcgta
    18301 cttgcagatc tgcaattttg tctcctccta tcccacggtt catgcattca aattccatcg
    18361 ttggatattg ggagattagt tttccggcaa tcaaaagtgg ataaccttta cctagatcag
    18421 ttgcttctgc tcggtatctt cctgcgtctg taatgctatc accgataaat aatatacgtt
    18481 ttttcatttg tcaaaaactc ctttttgaat aaaacgatga ttttcttcct tatattatcc
    18541 cataagccct atcaagacga aagagaaaat atatgtataa aaaatgacac cagcctagtt
    18601 ccttcaaaca attgaatgaa cgctcatcaa gtgtctagtt ttttatgcta tttttttctt
    18661 aacgtgccgg attccccttg attccttcgt tgtttggtcc agagagatcg atcacaccca
    18721 agcttccgtc aatcagacca ctgatgacgc catcgaaaca tatatttata ggctcatcta
    18781 agacttctag taaatgtccc tttactaata tctcttctag attgcttggg atagattggt
    18841 cataacttta tctggtccga tgatttcaag ggtgaattga acttacatat ggattattgc
    18901 atgctgaaaa tacggaccta ctccatcttc gaatgttact attagtttgt tttcaggtgc
    18961 tagatgctgc tttatttcaa cagcagaagc atcatcaaat cagatttcca tttgaattac
    19021 cttctttgta aatgtttgat tgattgcgat ttcgttatag aaccttgtga atcaaatagc
    19081 aatttctttt gctttcttct cttgtctgct ggaaacttcc attatcccgt atttaaaaaa
    19141 aagaactgcg agagttcgca gttctttttt ttaaataaac gttgctctcc ggccacaagt
    19201 cacaatagtc atcaaacatg aaaaactgtt ttttgaaatt actccttgtg actcggagct
    19261 gaacggcagg cgcacaacct cttatggata aattcggttc aacaaacgtg ggaatggaat
    19321 agcttcacgt acatggtcga taccagcaat ccaagtgact gttcgttcaa gacctagacc
    19381 aaaaccagag tgcggaacag aaccgtattt acgtagatca agataccatg aatattcttt
    19441 ttcatccaaa ccgtgattac ggatttgttc tagcaagaaa tcatagtccg ttgcccgttc
    19501 gcttccgcca ataatttcac cgtaaccttc tggtgcgatc atatctgcac aaattaccac
    19561 gtcttctctt gttggatgtg gtttcatgta gaatggcttg atggcttttg gatagtttag
    19621 gataaagaca ggctgttcga atgaattcgc aatgaacgtt tcatgaggtg aaccaaagtc
    19681 ttcgccccat tcgatgtcat caaatccatt tttcttcagt aattcgattg catcatcgta
    19741 agtaatacga gggaatggca attttgtata agtttttaag acttctttgt ctcgtcctag
    19801 tacatctaat gcgtattcgc aatgatctaa aacattttgt actaagaatg ctacatattg
    19861 ttcctgtact tctaaacttt cttcttgatg agtgaaagcc atttctggtt cgatcatcca
    19921 gaattcgatc aaatgacgtc gtgtttttga tttttcagca cggaaagtcg gaccgaatga
    19981 aaagactttt ccgaaagcca tagcagcagc ttccatgtat aattggccac tttgagataa
    20041 ataagctttt tgatcaaagt aatttgtttc gaatagatcc gtagtgcctt ctggtgcaga
    20101 accagtcaag atcggcggat caattttgac aaaatgatgt tgattaaaga attcatacgt
    20161 tgcacgaatg atttcatttc tgatctgcat aatcgcatgt tgtcttgaag aacgcagcca
    20221 taaatgacga tgatccatca agaagtctgt tccgtgttct tttggtgtga tcggataatc
    20281 gtggctctcg cttaccactt ccacatttgt aattccgatt tcataaccaa attttgaacg
    20341 actgtcttca cggatttctc cagttactaa cacggatgtt tcttggtgta gatttttagc
    20401 tgtttggaaa acttcttccg acacttcgct ttttacaact acaccttgga aaaaggctgt
    20461 tccgtctctt aattgtaaaa atgtgatttt tccgcttgat cgcttatttg cgacccaagc
    20521 gccgattgtt actgtttctc ccacatggtt tttcgaatcg atgatttgaa tttgttccac
    20581 gtatgactct ccttttatat tccttgaatt tcttcaggat taattttgac ttttactctc
    20641 aataaattgc ttgattctgc gtaccgcttc ttctaacgta gcgcgatcag tagcataact
    20701 catgcggaca ttttctggtg ccccaaagcc ttcgccagtc acaagcgcta cccctgtttc
    20761 ttctaaaaga tcttctaccc attgtgtgac attttcatat ttacacatgc gtagcgtttc
    20821 ttttacatta ggaaaaagat agaaagctcc ttgcggcttt tcaagagaaa cacctggcag
    20881 ttctgccaca agcggatata acgtattcag gcgttcttca aaggcgttcc gcatttcttc
    20941 tactgtatct tgctcacctt tcaatgcttc aacagctgca tattgactga ctgctgtcgg
    21001 attactggtt gactgggaag caacagcaat cattccattg atgatctcag aattccccac
    21061 cgcataacca atgcgccagc ctgtcatcgc atatgtttta gacacgccat tgatgatgat
    21121 ggtctgattt ttgattgctt ctgaaatcgt tgcaattgga gtaaactcat tgccattata
    21181 aactaaacga ccataaatat catcagcaac tatcaaaata tcatttttta ctgcccattc
    21241 accgattgcc tgaagttcat tcttgctata aatcattcca gtcggattcg atggagaatt
    21301 gatgattacg gcttttgttt tttctgaacg tgcttgttct agctgttcaa cagtaacttt
    21361 ccagctattg gattcttccc ctttcacaaa gacaggaagt ccttctgcca gttttacttg
    21421 ctcaccgtaa ctgacccagt aaggcacagg aatgatgact tcatcttgcg ggtcaaggat
    21481 cgtttggaaa agggtataca aagcaaattt cgctccgtct gttacgactg tttgtgaagg
    21541 ttcatagaca agtccatagt ttttcttcag ataatcaacg attgcctgtc gaagctctgg
    21601 gattcctgct gttggtgtat aatagctagc tttcccattg cggattgctt cgattgctgc
    21661 ttcttggatg ttttctggtg tcgcaaaatc cggttctccc acagtcaaac tcagcacatc
    21721 tctgcctttt gcttttaatg ccttggcctt tgccgcagct gccaaagtga cagacggttc
    21781 taatcgtttt gttctttttg ataactccat cccattcatt cctttacctt ttaaattccc
    21841 ttgattgtct tgacttcatc tccagattca aagccaatca ggtaataatt cagtttccca
    21901 tttgtttctt ttcctactac ttcccacaca ggctgatcat catacatccc taaagcagct
    21961 ttttcgattt ggatttcagg atgggccgca gcaattgtct gttttgcttc gttttcagtc
    22021 agtccctttg tttgatccaa tacttttact ttttcaccag atttaggaat gataacggcg
    22081 atttcttttc ctttatcatt tgtacctgtc agtgaaaagt atgtcttctt tcgagtaaac
    22141 caataaaaat gatcggtttc ctcaagattt gcatatttct ttgcgatttc tactgcttct
    22201 ttcttcgcct gcaccgccgg acgactggaa cgcaagtaaa aaatcgagct gatcacgatg
    22261 accgctaata aaaatacgat caagccaagt aacacgcgat taatcgtttt ttcctttgtc
    22321 tgttttttct tcaagggtcg tcgctccttt atcttcattc cttttcatta tagcagattt
    22381 taattatgaa aaaagacagc tcctctaact tccttcttca tttttcttta agaattcatg
    22441 gatatcctga tagatttcct ccatagctag ctctttgatc ggtacttttt tcggtaatgc
    22501 atggatgatc cgtgcgccat attttgcagt gactagtctt cgatccaaaa taagcaatac
    22561 gccacgatct gtctccgtcc ttatcaagcg acctaatgct tggcgtaact tcaaagctgc
    22621 tttaggtacg gattcctggt aaaaaggatt gatcccctct gattctaaat aagcgttcct
    22681 agctttgacc atcgggcgaa gcggattatc gaagggtaaa cgtgtgacta caacgatctg
    22741 caaagcatct ccaggcaaat ctacaccttc ccaaaagctg tcagcgccaa acaatacgct
    22801 tttttctgac aaaatgaagc gtttcaaaag cttctcccgg cttccaccaa acccttgagc
    22861 tagtatttct cgtccttttt ctagcataga cagatgcatt ttctgataga ccttctgcaa
    22921 aacatcatga gaagtgaata acacaagtat tggccgatct tcttgttcga tcagacctgt
    22981 aagtgctgtc tctaaataat cgatgtatgc ttgattgctg acttgatgga cggcaggtgc
    23041 ctcttttggc acatagatcc ttgcctgttt ctcataatca taaggagagg caatcacttt
    23101 aagaggtgct gcttcaatgc caagcctctt agagagatag tgtcgattag ggccgatctt
    23161 cagcgttcct ccaatataaa gaatacgatc atatcgagga taccaaacag tcgacggcaa
    23221 catagccgct tggaaatcat ggagatgaaa aatcatctgc tgcgctgtgt tcccaggaac
    23281 gaaccaatgg atcaaatgat ctcgccagtc ttccagccat tgatccatca gcaagtactg
    23341 cttgtctaat tgatcaaata agctatataa acttgctaaa tcgatccgca tctggtttgt
    23401 ccagctttct tggataatct caaaagtcag acgcatttgt gactgtagct gctgaatctc
    23461 ctgataaaaa agctgcaggt gatcaatggc tgtcccagat tccagtggca gctgatccag
    23521 catctctttt gtcatcatga cttcttctgg atactgtccg ttgggttgct tgccgctcac
    23581 taaactcatt aatccttctg ccatacaaat ctgtgcttcg catagcgcag aaagttcttc
    23641 tcgataaatc gataaccagc gtaaggcttc atgttctttc ggtaaatagc tttgtattcg
    23701 gtcaaacagc tgatttggtt cttctaattg ctggatttgt ttatggaaat agttaatact
    23761 taatcgtttg tcatttactt tttcagcgat ttccggcaaa tgatgtactt catcgatcaa
    23821 caaaaatgga ctagctggaa gcaatggttc ttttcgctgt gtttcttgta ctaaaaacgc
    23881 atgattgaca atcaaaaaat tactttgcct gattttcttt tgcaagaaac gaacaaagtc
    23941 ttgttcgtaa aacgactgat ttcttgctaa aaacccagta cctctgtgcg cgatatctgc
    24001 aaagaatgca tggttcaagt ttgttaaatg taattcatcc aaatcaccgg tcttcgtttg
    24061 cgtcagccaa actaagaccg ccatttgata caaagcatat tgtttttgtt ctggtggttc
    24121 cacaagtgtt cctttaaatc gttgcaggtc gatatagtgg cgataacttt tgataattac
    24181 aggttgtatg ggctgatcta gcaattgatt cagcaatgga atttcttttt ctatgatctg
    24241 ctgctgcaaa actagcgaaa cggtactgat gacagcgggt ttttcaggtg tagccaaata
    24301 actcaatggc aacagataac cgattgtttt acccattcct gtagctgctt cgatcatcaa
    24361 atcttttgat tcatcttttg tgaaatggtc gtagatcgca ttcatcagtc tgttctgttc
    24421 ttttcgatac atcagtgttt taccaaacat cttttctttt gcttgttttt ttcttggata
    24481 tgtccgctcg ccgtaatgga cggatgtgaa tagttccact tcttttttct gcaaagctag
    24541 cccatcgacg atttctaatg aggaatcaag tggctctggt ttttctttca tttcttcaat
    24601 cacatgataa atgaaacgac tcgtatccat acctgtgtgt ctacttaatc gcgcaatctg
    24661 ctctaacgtg atgattggca attttctcat ccgttcttcg atcagcaaaa gaagttgacc
    24721 tgtcacttcg gcatcactat ctgcctgatg aggattctca tgaataaatc caaggctttc
    24781 tgacaaatca gctaagcgaa agcttggttc cgtcggcaag aaaatttgtg caagttctac
    24841 tgtatcgatg cctggtatct tcaaactagg aagtccgcat cgcatgagtt catggttcaa
    24901 aaaattataa tcaaaataaa tattatgcgc gacaaatatg gtgtctgcca aaagattgta
    24961 aatcgtttgg gcgacatctt cgaaataagg cgctttttgg acgcgttgat tcgtgatccc
    25021 tgttaaatgc tggatttgtt ttgaaatctt ccgccctgga tgaatatcga tcgaaaaacg
    25081 tgaagtgatc cgacctgatt caatcatgac gcagccaaat tggatgatcc gatcttcttt
    25141 tggattcgtc ccagtcgttt cgatatcaac gactgcataa gttcttggtt tcaaaatatc
    25201 ttcctcttct cctgcctcta atgttctttt gaaattatac tacaaagtgc agaaaaatac
    25261 tctcatcgct ttttaaagaa aatagacaaa aacgcccaga cacgcaaatg tctgggcgtt
    25321 tattt
//
LOCUS       DACEQH010000041        24665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.041, whole genome shotgun sequence.
ACCESSION   DACEQH010000041 DACEQH010000000
VERSION     DACEQH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.041"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            236..1042
                     /locus_tag="IW833_001751"
     CDS             236..1042
                     /locus_tag="IW833_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1307870.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1054..2274
                     /locus_tag="IW833_001752"
     CDS             1054..2274
                     /locus_tag="IW833_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1307871.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2264..3850
                     /locus_tag="IW833_001753"
     CDS             2264..3850
                     /locus_tag="IW833_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1307872.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3889..6027
                     /locus_tag="IW833_001754"
     CDS             3889..6027
                     /locus_tag="IW833_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1307873.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6315..7706
                     /locus_tag="IW833_001755"
     CDS             6315..7706
                     /locus_tag="IW833_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1307874.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            7765..8574
                     /locus_tag="IW833_001756"
     CDS             7765..8574
                     /locus_tag="IW833_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1307875.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            8546..9535
                     /locus_tag="IW833_001757"
     CDS             8546..9535
                     /locus_tag="IW833_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1307876.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            9649..10644
                     /locus_tag="IW833_001758"
     CDS             9649..10644
                     /locus_tag="IW833_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1307877.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
     gene            10648..11790
                     /locus_tag="IW833_001759"
     CDS             10648..11790
                     /locus_tag="IW833_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HAQ1307878.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
     gene            11787..13214
                     /locus_tag="IW833_001760"
     CDS             11787..13214
                     /locus_tag="IW833_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1307879.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(13281..14570)
                     /locus_tag="IW833_001761"
                     /pseudo
     CDS             complement(13281..14570)
                     /locus_tag="IW833_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(14750..15160)
                     /gene="tagD"
                     /locus_tag="IW833_001762"
     CDS             complement(14750..15160)
                     /gene="tagD"
                     /locus_tag="IW833_001762"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAQ1307880.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            15286..16677
                     /locus_tag="IW833_001763"
     CDS             15286..16677
                     /locus_tag="IW833_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAQ1307881.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            16810..17757
                     /locus_tag="IW833_001764"
     CDS             16810..17757
                     /locus_tag="IW833_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ1307882.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(17797..18957)
                     /locus_tag="IW833_001765"
     CDS             complement(17797..18957)
                     /locus_tag="IW833_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1307883.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            19122..20297
                     /gene="mltG"
                     /locus_tag="IW833_001766"
     CDS             19122..20297
                     /gene="mltG"
                     /locus_tag="IW833_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ1307884.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            20433..20912
                     /gene="greA"
                     /locus_tag="IW833_001767"
     CDS             20433..20912
                     /gene="greA"
                     /locus_tag="IW833_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ1307885.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            21080..21811
                     /locus_tag="IW833_001768"
     CDS             21080..21811
                     /locus_tag="IW833_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ1307886.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            21808..22875
                     /locus_tag="IW833_001769"
     CDS             21808..22875
                     /locus_tag="IW833_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1307887.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            22882..23514
                     /locus_tag="IW833_001770"
     CDS             22882..23514
                     /locus_tag="IW833_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1307888.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            23654..24319
                     /locus_tag="IW833_001771"
     CDS             23654..24319
                     /locus_tag="IW833_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ1307889.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            24347..24640
                     /locus_tag="IW833_001772"
     CDS             24347..24640
                     /locus_tag="IW833_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ1307890.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
BASE COUNT         8419 a         3662 c         4781 g         7803 t
ORIGIN      
        1 aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaccaaca caaaatatta
       61 tagaacccct tttttggttt attgcaattg tctagcatct tctgtaaaat agaaacgtta
      121 gagaaaatct tgtatcatag aaaattaaga aagaaataag aacgcagctc ggtaccaaaa
      181 atatggtaaa atggcacttg actgtaattc actgaaatcg cttggaggaa cacaaatgtt
      241 taaagatttg tatttattta tgaaagacat ttatcagaat agaaaattac tgctacagtt
      301 ttcgatcaat gacttcaaat caagatatgc aggatcgttt ttaggaatct tatgggcgtt
      361 tatcaatccg ctatttactg tattgattta ttggctggta tttggtgttg gactgaaagc
      421 acaaatgatc gatgcaaaat acccatttat cgtatattta gttaccggga tgattccctg
      481 gttcttcttc tcagatgcat ttgcatcaac tacggttgtt tttcgggaat atacttattt
      541 agtaaagaaa gtcgtattca atattcgtat cttgcccacg acgaaaatcc tatccaatct
      601 gtacacccat ttattcttca ttctgattgg catagctgtc gcgcttataa atggtatata
      661 tccttcagca atgtctttac agctgattta ttatttattt tgcatgtgtg ctttcttgac
      721 aggattgact tggctgacgg catcgatcca accattttta cctgatatca tgcaattaat
      781 tacgatcgtg ctgcagttga tcatgtggac attaccgata ttatggagcc ctaatcagtt
      841 tgattcaggt cttgtaagca tattgaaatt aaatccgctg tactatgttg ttcaaggtta
      901 tcgtgagtca tttttaagtg aagcttggtt ctgggagcat ggaacatata ctttgtattt
      961 ctgggttttc acgttagtaa tgttcgtaat cggctctgca gtgttccgta gattgaaacc
     1021 acatttttca gatgtgctgt aaggagaata gctatgacag aatatgcaat tagactaaaa
     1081 gatatcacaa aatcttataa tatgtacgca aaaccttcgg atcgatttcg tgaagccctc
     1141 aatcctttta agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt gaatgtagaa
     1201 ataaaaaaag gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa atctacaatg
     1261 ttgaaaatca ttacgggagt actgacacca acatccggca cgatggaaat cgaaggaaaa
     1321 atctcagctt tgctagaact aggatcagga ttcaatcctg agtattctgg ctatgaaaat
     1381 atctatttaa atggaatggt tctaggattt tcaagagaag aagtcgatga aatggtcgat
     1441 gatatcattg aatttgctga tattggcgac catatttatc aacctgtaaa aacctattca
     1501 agtgggatgt tcgttcgtct agcttttgct gtagcgatca atgtgaatcc tgatattttg
     1561 attgtggatg aagcattggc agtaggagat cttgaattcc aactgaaatg tatggaaaaa
     1621 tttacagaaa ttaagaactc gggaaaaacg attttattcg tttctcatga tattagttcg
     1681 attcgccgtt tttgtgatcg ttcctattgg ttgaaaaatg gagaagttgt cgaatatggt
     1741 gatacgatgg aagtaacgac gaattacgaa aactttttga agaaaaaatc agttaaaaca
     1801 gtagatcgaa ataaaaacca agtagaacgc ttcgcagctc cagatatcgt agacgtgacg
     1861 aaagccgaat tgttggataa aaataaaaat ccagtggata tgctggaaca aggagaaaaa
     1921 ctatacgtta aagtaaccta tgaagtaaaa gacgatacaa tcaaaaatcc agttttagga
     1981 attgctctgc gtacagtcga caatttttat gtgtgcggac tcaatactct actagatggt
     2041 atcaagatcc cttggaaaaa aggtgagaat atcttttatc tggagtatga aaagttaggt
     2101 ttgttagctg gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc aacggttcct
     2161 ttggtataca agacaaaata catgaacttg tttgtcagcg gttcttatat cggagaaggc
     2221 atcgtggtct tagaccataa atgggaagaa gggactcata gcaatgaaat atgattttga
     2281 aatggaccta gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca aaccaggaag
     2341 taaagtttta gagtttggtc ccggaaacgg tcgattaacg aaacatttga tcggagcaaa
     2401 acaatgtgaa gtgagcattg ttgagctaga taaggaatta ttcgattttg tcagcgaatt
     2461 tgcccaagat ggattttatg gagatatcga aagttttgaa tgggcaaact attatgctgg
     2521 acagacattt gattacgtct tgtttgcgga tgttttagaa catttagtag atcctggaaa
     2581 aacattgaag aaagtcagag aatttttgaa tgaagaagga gagatcctga ttacattccc
     2641 taacttagca cataattctg tcatgatcga tttgttcaat aatcaattgc cttgggcttc
     2701 ttatggtctc ctagatgaaa cccataattc tttttatact catgatggtt tccaaaaagt
     2761 ttttgaaaaa gccggattat tcattaatat tgaagattac ttgtacttgg ctgtaggaga
     2821 tacagaatta aaatcaactt atgaagagtt gccggaagct gtacgctatg atttcaaaat
     2881 gcgaccattt ggtgaagtct accaatactt tttctcattg atgaaacatc cagtagtaca
     2941 aacttccatt gcacagccac aaaactctaa ttatgtaaaa gtactagaag taacccagca
     3001 aacgaaacaa gatgaaacaa tccaacaaat accattcaat aatttcacag gggaaaatga
     3061 gacactaagt ttccccgtgt cagaagcaac agaaagaatg gtgtttcgct tcgcacagca
     3121 acctagcttt atcgaattca gtgcagaagc tgctggagaa aaactcatat ttatcgattc
     3181 aaatgctgta gtcaaaacag taaatgattg ttatttgttt gatgggaaag aaataccaga
     3241 gttcgctcta acggatattt ctgggaaaga agtgactatc cattgtcact atcgctttat
     3301 cggggaattg acaccaacaa tgaaagaatt gctggaaaca attcgtccta tggctgaagt
     3361 cacaaagcag ctttctgaga aaaatgatga attagaaaga gaaaatcagc atcttcttga
     3421 ggaaaataca agactagacc atacgttaaa aacaaccaca aatcgctatt gcacattgtt
     3481 ggaaagtgat gaatggacga tcaaacatag actaggacga cgtaagaaaa aaacatcgaa
     3541 aaaaatacaa gaaaaagagc tttctatatg tattgatgag aaaatatggg atgcagaaac
     3601 aaaaatatta aaaatcatcg gatggggaat cgctaattcg gatcgccagc cattgtccta
     3661 taagttatca gctgaccaat cgccattttt cgaagcaatt cctgtttcac gagatgaagt
     3721 caatcaagca gaacagctcg caaaaggaac agaagcggga tttgaattac gaatcctttg
     3781 cgagcaagaa cgttcattct tagtggaagc agtagctcaa aatggtcaat catggatcat
     3841 tgaaatgtaa ataatgatta acttataaag ataaggagta aacgaatcgt gcaaaaaaaa
     3901 gaaattgccg tatttatcga tcaaatcact cgtgaacggg ccactggcga tttgcttatt
     3961 gtgggctggg caattgatga agtgaccaaa gaaatcccta cgataaaggt agaaaaagaa
     4021 aatgtgattg ctgaagcaac acatgtcgta agattggata tcaatcattt atataatctt
     4081 gatgttaaga cccaaagcgg ttttaagata cgtttatccg ggaaaatgag aggaaaagcg
     4141 atcttagatt ttcaaacggc aaaacatcaa aatggcatag ctgtgaaact aaatggtaaa
     4201 tacccttatg atgacggcat tgaatcttca tgggaaagaa aaaaaagatt actgaaaaaa
     4261 gggataaatt atgcaaggac tcatggcgta aaaaaaacaa tccgccgggt caaactggaa
     4321 ttgaaaccag gatcgatcga ctatgcagag tggatcagtc gtcacgagaa acctgatctg
     4381 aaagagcaaa ggaaagaagc acaagggttt gcataccgtc cattgatttc catacttatg
     4441 cctgtctata acgttgaaat caaatggctc gaaaaatgca ttgattcggt attggatcaa
     4501 acgtatgatc actgggaact atgtatcagt gatgatgctt ctactgatcc agctatccga
     4561 aagtgtctgg aatcctatca agcaaaggat gatagaatca aagttgtttt ccgacaagaa
     4621 aatggacata tcagtctggc aactaattct gcgttggaaa tggcagaagg agaatttata
     4681 gcgctcttag ataatgatga tgaattaccg ccatttgcat tatatgaagt ggcaaaagtg
     4741 ctaaacatac atccagaact ggatttgatc tacagtgacg aagataaaat cgatgcagat
     4801 ggcaatcgct ttgatccgca cttcaaagca gactggtctc ccgatacatt gatgggaaat
     4861 aattatatct ctcacttagg cgtatatcga acaagtattg tgaaaaagct tggcggtttc
     4921 cgtaaaggct acgaaggctc tcaagattat gatttggtcc ttcgtgtgac agaacaaatc
     4981 cctgcagatc atatttacca tattgaccga gtcttgtatc attggcggac gatcccagga
     5041 tcaaccgcaa gtaatggtga agccaaaagt tatatctatg attctggagt gaaagcattg
     5101 acagatgccc tgtctagaag gaatatcaaa ggaagcgtac gtcctggcag aatttctgga
     5161 ttttatgaga ttgcttatga cgtcttgcaa gaggatttgg tcagcgtgat cattccaacg
     5221 aagaatggct atgaagatct aaaaacttgt gtggattcga ttatcgaaaa aacgtcttat
     5281 ccaaattatg aaatcattat tgcagataat ggaagtacag atcctaaaat gcaggaatta
     5341 tttgcagaat acaagcatca gctgaaagac cgatttatcg ttgaattaat tgacattcca
     5401 ttcaattatt cacgtatcaa caatctggca gcagaaaaag caaacggcaa atacttttta
     5461 ttcttgaata acgatacaga agtgattgaa cctgactgga tgacagcgat ggtttcttat
     5521 gcacagtttg atcgaatcgg ctgtgtagga gcaaaactgt tttatccaga tgatacaacg
     5581 cagcatgcag gagtattatt aggaatcggt ggtgttgcag gacatgcatt gaataactat
     5641 gatcgaacac attgtggtta tttcggccga ttggtcattg acgtcaatta cttagcagtc
     5701 actgcagctt gtatgatggt caaggcagct gacttcaatg ctgtcaatgg atttgatgaa
     5761 acattagaag tagcattcaa tgatgttgac ttgtgtttga aagtttatga actgggtcgt
     5821 tacaatgtct atgcacatca agcagaattg tatcatttcg aatctaaatc aagaggttat
     5881 gaagatacac cagaaaaaca aagacgtttt gccggggaaa taaaaaaaat gcaagataag
     5941 tggccttctt atattgctca tgacccattt tataacgata atctaactaa agaaggaatc
     6001 ggcgattttt cattgagacc tgattgatta aaagaactgt attttcattt ttattgatga
     6061 aaatacagtt tttttagtat atgaaaattg taacttgtga ccgaagagat gataggaaga
     6121 acacccattc agtaggaaga atgtgatacg atttttgtcc aactctctgt taggcagaaa
     6181 tatacatata aacatacaat agtcaccttt tctagtttct tttaatttat tttacaattt
     6241 ggttattcac taggttttta aactttgcta tggtaagatg atagaggcat tattttatgg
     6301 ttaggagtgt atatatgaat aaaaatgggg agtggaatgc ggcacgtagg atattcatta
     6361 tcatcatgga tgtacttgta ttgaatgtgt cgatctatct tagctttttg ctgaagttcc
     6421 aaggagaagt cccagcaaga aacttagaga cctttcagca ttcagcagtt tttatttcca
     6481 ttatctttat tgcgttgaat attttgttag gagcttatgt attctatagc cgagtgatca
     6541 gtgatatcgt cttcattact gttattggac aggtgctgat agcattgggg atcatggtag
     6601 ttacttttgc tggacgttgg tttgcatttc cgcgtggcgt catcgtgctc tcgtttatac
     6661 ttggcacgat attacttagc gtttatcgga tagttgttta taaactatac ttaagagtca
     6721 gtcgtgataa gaaagtcctt atcgtaggtt tggagaaaga tgtcgcacca gctatccaaa
     6781 actttcaatc taagaaaaac aacaaacata aagttgaagc agtagtgatc gacaagtatt
     6841 acgaaaacac aaaaaaattg attgatgata tcgatattgt ctacttggct tctaaaatcc
     6901 cagaagacga aaaaatcaat atttatcaat tagtaactaa aaaagagaag aagctatttt
     6961 taaatacaac ttttgaaaat ttaacgatga tcaatccgaa catcatgaat ttcgaagatg
     7021 aaagtatcat tgaagcttcc ggtttcaaaa ttcagccgga atatgaatta ttcaaacgtc
     7081 tttttgatat catcgtttcg cttattttac tgattcttgc ttcacctttt atgctaatca
     7141 cagcgatttt ggtaaaaaca acttcacctg gaccaattat ttataaacaa gtacgtatta
     7201 cgaaaaatca aaaggaattt tcaatctata aatttcgttc catgagtgta acagctgaag
     7261 caaaatcagg accggtcttg gcaaagagca atgatgcacg tgtgacacca gtaggcaaat
     7321 ttatccgtgc cgtacgtttt gacgaattac ctcaaatctt taatgtattg aaaggcgata
     7381 tgtcgatcgt cggaccacgt ccagaaagac catttttcgt tgatcagttc aatgaagaaa
     7441 atccttatta ttatttaaga cacaatgtac gtgcagggat taccggatat gcacaagtat
     7501 acggaaaata tgtatcagat tataatagta aactacgttt tgatttgttg tatatcaaaa
     7561 agtattcctt actattagat ttgaagattt tacttcaaac aatcaaaatc ttatttgata
     7621 agatgtcctc acaaggacta gatgaagatg aaaatatccg ttcgttagca gaaatcgact
     7681 tttcacaaga gaaaatttat cgctagatgg gaaaaatttt gagcaaagaa gtaggagaga
     7741 acattggaaa ataaaaatat aagtatgctg ctttctttat atgataaaga aaaaccagag
     7801 tatttaaagg aagcattaac tagtattttt gagcaaacag taatgccaaa agaaattgta
     7861 ttagtttatg acggccctat aaatgaaaaa cttcaagtag ttgtcactta tttccaacaa
     7921 aaagatcctc acttgtttac agttgtaaaa ttagaaaaaa atcaaggatt ggggattgct
     7981 ttagcaattg gattagaaca tacgaaaaat cgcttagtag ccagaatgga tacagatgat
     8041 atcatggaac cccaacgatt agaaaaacaa ttagagatct tccaaaaaga tccagaaata
     8101 gcgattgttg ggagtaatat tgaggaattt actggcacgc ttagcaatgt tatcggaaga
     8161 aggattgtac ctgaatataa tgatgaaatc cgtgtgtttt cgaaaagaag aaatcccttt
     8221 aatcatatga cagtcatgtt tgataaaaat gctattttag aagtgggaaa ctatcgccca
     8281 ttaattggat ttgaagatta ttatttgtgg gtaagactgt tgaaggcggg atataaaggg
     8341 tataatatcc aagagattct agtccatgct agagcaggtg ctgacatgta tgctagacgt
     8401 ggaggaagta aatatctttt accaggaatt aaaggccgat atcgcgtatg gaaagatggt
     8461 ctaggatcac taaaagattt tctgatagtt atttgcggac atcttgtaat tagtttatta
     8521 ccgaataaaa ttaggggaaa agtatatgaa tcaaaactta gaaaaacaaa ctaaactttc
     8581 tataatcata cctttttaca atgcaaaaaa tactttaaaa agagctgtag acagcgtctt
     8641 aacgcaaggt tatcccaata ttgaaatctt gttaattgat gatggatcta cagatggtag
     8701 tagtgacatt ggtgacgaaa ttgctaatct agagagtaaa gtagcatgtt atcacttgga
     8761 aaacgctggt ccatcagttg ctaggaattg tgggataaaa gaagcaactg gaaatttggt
     8821 aggattttta gatagtgacg attatttttt acctgattgt tttaataatg ttttgaaaga
     8881 ctaccatagg aattaccccg atatcattgc ttttggatta agtaaaggaa aaaatataga
     8941 aaatgcaaat ttatttgttc ccgaaaagaa gattggttgt tcaagtgaag aagcaattga
     9001 gcaaatgttt agaagtaagg cagtggattt ttacgtatgg aataaatttt ttaaaaggga
     9061 attattcaat catatagttt ttccagaaaa taaattatat gaggatatgg ttcctatgta
     9121 tgaagctttt agagcggcta gtaaaattga tattttgcca ttttcaggta ttttttatta
     9181 tcaaaataat gatagcatcg tttatcagtc ttttaattca aaacagtatg ataatatttt
     9241 gcaaagaaaa atattagttg atgaagtaaa gaaagattat cctcgactta ttaatttggc
     9301 caatgctcga ttagtagacg gatacctttc aacagggttt aaaattactg gcaagaatca
     9361 aaatcagaaa gaaaagcaaa aatattataa tcagtctaaa agagaaataa aaagtatata
     9421 ttcaaaaata agaagtaaca atgagatatc aattgctaaa aaattagctt tatttttata
     9481 cttaattaat cctaaaactt attttatttt atataaaaaa gttctaaaaa aataaagttt
     9541 ttttcgatat gttatttttt atgtttatta acttcggaag actaaaattg ttactggtaa
     9601 tgatacattt gtaaaaataa aaaagatgat ttttaggaag tgaagagagt gcataaaatt
     9661 agtgttatag tacccgcata taacgtagaa gactatttag aaaactgctt agaatctatt
     9721 tccaaccaga cacattcaaa tattgaaata atagtagtca atgatggttc cccggatcga
     9781 agtggggaaa ttgcagaaga atataaaaag aaagatatgc gattaaaagt gatacatcaa
     9841 gtgaatggtg gtttaagtgc ggctaggaat tctggtcttg attctgcaac aggagatttt
     9901 gtgtgcttta ttgacagcga tgattggata gaaccagact atcttgagtt actatatttt
     9961 ggcacgcaaa cttatggagc agatatctcg gtgctaaaaa taaaaaagat ttcaagttta
    10021 gaacaactaa aagaacatac aattgctgag agaaaatggg aaatatttga tagagaacaa
    10081 gcaatgaaaa acttatttat aaataataaa attggctatt ctgcaaataa taaattattc
    10141 aatagaaatt tatttgataa tataagattc ccactgggta aattaatgga agataaggct
    10201 actacttatt tgctgatcga tcattccgaa aaagttgctg tcaattcatc tgctaaatat
    10261 cactattacc aaagccctaa aagtatactg agaggaagct tcaatcctag aatttttgat
    10321 tcttttgaag tccatgaaga tatactaata ttcatagata agttttatcc ggaattatca
    10381 attaatgtta gaactcgcta tgtctatgaa gcaataagga tgatgatgac aatgattaaa
    10441 aataactata ccaataaaga agattattta cgttctataa atattttaca acgctttgaa
    10501 aaagaagtga aaattgatcc cgacttttca ccaaaattaa aaaaattagt aaagttttta
    10561 ttaataaata aaaacttgcc ttactatatg tcaagtagta agatagcaag taaattcttg
    10621 attaagatag aatctgtgag gtaagagatg aagaatataa aaaaattttt ttcaaggaat
    10681 attttcccgg tttttattaa aatattacct attcaaaaaa atttaattgt attttcaagt
    10741 tttggcgggc gtaactattc tggaaatcca agagttattt cagagaaaat tcatgaaaaa
    10801 aaattgaatt acagaattat ttggttaatc aaaaaagaag cgattataac tgatttacca
    10861 gattatatta aaagaattga ctccgattct tttttagctg ccttttattt atatacagct
    10921 aagtattgga tagacgatag tcggaaaacg attaagtatg caaaaaggaa aaaacaactt
    10981 tattttcaaa catggcacgg aacgccttta aaaaaaattg aatttgatgc aaaagataag
    11041 ttacctaaac gttacctaag ctatgcgaaa aaagatagtg agtcaattac atatttatta
    11101 tctggtaatc gctatagttc taagaagtat gtttcagcat tcaatatcgt tccttctaaa
    11161 atattagaag taggaactcc tagaaacgat gaattagcct ctagtaaaga gcaagtgttt
    11221 gacttgaaaa agaaacaaat caatgtctta tttgctccaa cttttcgaaa taatattgag
    11281 gacaacggaa ttacgcaact agaatggtta ggtgtggata acttacgtga attttttaaa
    11341 aagcaacaac aagaattaaa ttttatgact aaatttcatc ctaatgttca tagtaaatta
    11401 gcaactgatt ctaaaagtat agaatatatg aaaaaacatc aaattacttt aattaatgat
    11461 gggattcctc ttgaaatgct ttttgaagag atagatgttt taatcacaga ttattcatct
    11521 atttttttcg attttgcgct aacaaaaaaa ccaattattc tttttaacta tgatgaaaaa
    11581 gaatatagct tagaaagagg tttttatata cagttaagtg agttaccaat tataagcgta
    11641 caagatagtt atggccttgt tgatgttttt aaaaaccaac aagaaaagct tttgaaaagt
    11701 tctcaagagt tacttgatta tattggaaat tatgaaacag gagcaagtac agaaaaggtt
    11761 cttgagttga tagagaggga tataaagtga aaaaaacagt aaaagatata ttttataatg
    11821 cgatatatca agtctttttg attgttttac ccttacttac tatacctata cttagtcgta
    11881 gaattggttc aactggttta gggatttatg ggtatgtttt ctcaatatca cagtttttaa
    11941 tgactgtcat tgctgtggga atgaatcctt ttcgtattcg aaatatcgct aaatcaagga
    12001 aagataaaaa agcactatct ttacaattct ggaatattta ctttattcaa tttttaatag
    12061 gcttgagtgt ttcctttctc tatatagcct atatttattt ttttgatgta aagtataaaa
    12121 atttatactt aattcaattg atatttattg tagggctaac attagatatt tcttggtttt
    12181 ttcaaggaat tgaagaattt tcgaaagtgg ttattagaaa tacagttatc aaattatcat
    12241 cggtattgtt aataatcatt tttgtttcaa ctaaagatga tttgtggatt tacgtgttca
    12301 ttacttcaat tactaacttt ttaggaagtt tagttttctg gttaagtatc cacggaaaag
    12361 taggtaaacc aatctttaac tctaatgttt ttaaacagtt gtggaaacca gcttttatta
    12421 ttttgactcc tcagttgttt atgcaagtgt acacaacttt agacaaaacg atagttgggt
    12481 attttgtgga tccaacggaa ttatcttatt atgatcagtc tcaaaaaatt gctcgtatta
    12541 tattggcagt cttaacatca attactattg ttatgttgcc taaaatatct aaagcacagg
    12601 cagaggggaa atcagaaaat attttagttt acacaaaaaa atcttttgat tacacgatta
    12661 tcattgcatt aattcttttt agtgtggtgt ttatcaatac aaaagaattt gttccatggt
    12721 tttttgggga gaatttcaaa ccgatgacaa taaacatgct tatttcttgt ctaattatag
    12781 ttgttagtcc aattggtgga gtttttgcta accaatttgc aattgctata gaaaaagata
    12841 aggagtatgc ttatccaatg atcattggtg cgattgtaag tattattgga aatgtagctt
    12901 ttgtgcctac ttatggcgct gtagcggcta cgatcgtact cgtggtagta gaattattag
    12961 tctttttctt tagagtattt tttgtacgaa aagaaataaa aatatcctat ttctttggga
    13021 agaacgttat cttatttttt atagtcaatc ttactttgac tacttgggcc tattactttc
    13081 ttccacaatt attctcttct tcatttttag atatggcaat taaatcaata atgattttca
    13141 ttatttatct tccgtttgtt tattttcttt ttccagatat acgaaatgaa ataagaaaga
    13201 ttactcgaaa ataaaataat gttgtaaaaa agtagttctt tgtcaaatcg ggctgataag
    13261 gtcatttatg aacaacaaag ttagtaagag ttactagctt ttaatgcata gctgattaac
    13321 ctgttgatta gaaaaatcca caagcgcata ttggtaaaac tgcgaaaccc ataagcaaat
    13381 cgtttcaatg ttttgatatg tgtgcgaaga ttctctaatt ttgttattag aatacttttt
    13441 cgaaaatgct agttaaaagc ctttttcaga tatgaaaaag gctttttgag atagtgaagt
    13501 ttcgagggaa cttctttggt cgtttctcga agtagctagc tgtttcttgt gataattgct
    13561 aagcagcttt tgataaactg cttagcaatt ttaagttcat aagagattga gggtagacga
    13621 tcaactactt cactttctgt taaatagcgc cattgaaaat tctgccattt cttgaatgtt
    13681 gtgtaatcca actcgtcatc tgattttaga agtaatttat acaaaggttt caattgtcga
    13741 tagtacttag cttgtgaagt gtctttttga ttcaactgct tcatcacacg ttttcgaaca
    13801 cgatcgaaaa acttgttcaa gtgttgtcca atgtgaaagc aatcaatgat gatttgggcg
    13861 tttggaaagg actctttgac taatgaaaaa taaggggcat tcatatttgt gacaatgtat
    13921 cgaaccccca tatgagcttt tcttgtaaaa cgcatgaaat aagcaatcaa atcttttttt
    13981 ctccggctgg acaaaatgtc aaagagcttc cctgtctctc tattcataca agaaaaggag
    14041 aactttccat gggaaattga aagagagcga aactcgtcga atagtaaaca atgaggtaaa
    14101 tagtctagca tctgtcgcag cattgccgat agagcagata atgtccgttg aaatgtcgtt
    14161 actgaaagat cacaacgttt cgtaagtgtc tttaacgctt gacgctcggt caactcttct
    14221 aatatcgtgt aatgaacttg tttcgaaata aaacatgaag gaacgacaag atacgtatga
    14281 gcagtaaaat attttccgca tgattggtag tggtaatgtt gttttctaag acgcaaatag
    14341 gtttatttat tcccactgcg atttaagata atcattgaaa tcttcttgcc gtttttcacg
    14401 agctgcttcg cagtttcttt acaaaataga caatgtctaa aagtgcgaac taatgtacct
    14461 tcgattacga tggctttctt gtccttataa aaagtttttt gaaaagaatt aatttcaata
    14521 tttttatctt taatagtgaa gtgctctttg atagaacgag aaatggatat acaaaatctt
    14581 cctttcagat tgatgtttta gacgactcta ttttatcgaa atttttgtat gtctgttttt
    14641 tttacaagaa aatctaggat cgacagaaaa ttctatcagt cctagaaatt atagagccat
    14701 ttttttacgt cttatccaga tatattgttc tttcattgtt taaaaaatat tatttatcac
    14761 tatttaaaga attaagttcg ttctttattt gagttgtaga gatctcaggt gttcttggta
    14821 aataaattac ttgtgcatct gttagttgtt caagttcatc aaattgtcca gcccaatcat
    14881 cgcccatcac gaaataatct gcatggtatt cttttatatc ttttactttt tgttcccaat
    14941 tttcttcagg aataactaaa tcaacgtagc gaatagcttc aagtaactgt tttctttttt
    15001 cataactaaa ataacatttt ttttgtttcg cattccaatt gaattcatct gtagaaaggg
    15061 caacaataag ataatctcca tattgttttg ctcgtttcag taaattgata tgcccgtagt
    15121 gaagcaaatc aaaggtaccg taagtaatta ctcttttcat ctttcacctc tcgaaattca
    15181 ttaatataca ttgttagttt agcatttttt tgttagaatt gaaagaacca gtttatccta
    15241 aaaaggataa gaaatgttta tgagaaatag gtggatggag gaaaaatgtt gaatgaaaaa
    15301 tttttaaaat atgtgaaagg ttggtatttt atttattttg gttttattct aatgttacga
    15361 gcaataggcc cgtatttact attacctact agatatgata ctttactttt ttggcctgct
    15421 ggaatagcgg gagttgtctt gatcattata gatgcaatta ttgcatttag agaaaaaaat
    15481 cttaaaaaat atgattggct attaattgct tttattggcg cgatgctagt aagcagttta
    15541 atgaatttaa aatatggata tagcgaaaat atcaagttaa tcatgtggca attaatcttt
    15601 ttctttatga tttatcagtt tggcaaagac tacagtagta actttttttt caaagtattt
    15661 acttggatac tttcattggt atggttttta gctaatttag tatcattata ctggtttgtc
    15721 attcaatatg gcataaaaat tccaataaaa tataagtatt acccagttcg tttagggtgg
    15781 ttaggcaacc gtttatttgg tgtatataca gatccaaatt ttggtgcggt tatatcatta
    15841 attgtcatca ttattgctat atattatttg gtcacttaca agcatttaaa tcttctattt
    15901 aaaatatttt ttgcaattaa tatttttatt caattctcgt ttattgcttt atcgggctca
    15961 cgcaccacat tagtttgttt attaatagca acttttgttt ttagtttctt tacaagttat
    16021 agtagtcgtt ttcttaaaac taatacttta aaaagatggc taactgcatt agtagtagga
    16081 ttgctaagta taattgcgat gtattatgta acagatataa ctgctcaagc atgggcacat
    16141 gttccgcata ttacagaaat aaaaaaagat atatctgatt catcaggaac aactagcagc
    16201 aataaagttg atttaaaacg ccctgacact gatggcgggg atgtttctaa tttaaggttt
    16261 ggactttgga aaagtgcaat tgaaatattc aagagtaagc cgatatccgg cggttctcca
    16321 ggatactata ttgaatacgc acatgataaa ctaccatata cattaatggg aaaagatact
    16381 ttaactgctc ataattttat tttcttagtt atggctgcta caggaggatt aggtctattg
    16441 acattgttaa ccttcttatt tatccaatgc tttaaaactt tgcgttattg tttcacacaa
    16501 ccaaatctag taaaagataa gcactttttt gctattttaa tgattcttac aatagcagtt
    16561 tcggcattat ttatcacaga actcattttg gttagtacga taggttcatt tattttttgg
    16621 ggatatctgg gttctatcca aaaaactata tctaaagaag aaggagtaac taattgaaac
    16681 tatttttagg tatataaagt agaaaagaaa actagttatc ccaacttcaa caaattttat
    16741 agtttttaca atataaaaat ggtaaactaa ctttattaag tatgagaatg cataattgga
    16801 gtgaatttga tggatactat attgattact ggtggagctg gttttatcgg ttcaacgcta
    16861 gcgaattatt tgggaaaaga aaacaaaatc gtagtagtcg acgatttatc aatgggaaaa
    16921 aaagaaaact tggatatgga gaaacaagtg actttcattg agggggatgt atcagatcct
    16981 caattgatgg aaagaatcat gaaacagtat cagtttgcct atatttttca tttagctgct
    17041 gtagctagtg tggcagattc tgtagaacgt cctctagaaa ctcatcgtgt aaattttgac
    17101 agtgcgcttt tattgcttga attggtacga aaatatcaat ctggtttaaa acgattggta
    17161 tttagttcat cagctgcagt ttatggcgat gaaccaacgt tgccaaaaaa agaagaatcg
    17221 gtcatccgtc ctttaacacc atatgccatc gataagtttg catcagaaca atatgttttg
    17281 gattactgtc atttatatga tgttccgact agtgctgtcc gattttttaa cgtttatggt
    17341 ccaaatcaaa atcctaactc tccatatagc ggagttattt cgattctagt ggaccgatac
    17401 aaaaaacaat tggcaggaga aaaaacagaa tttactttat ttggtgatgg tagccaatct
    17461 agagacttcg tttatataga agatgttata caagcacttt tacttgttgc taaggaagaa
    17521 aaagctttag gacagcaatt caatgtcggt acaggaaaaa gcactacgct acttgaattg
    17581 attcattcaa ttgatcaaat cttaggaaca gagctagcgc ttaaatatga agcggagcgt
    17641 tcaggggata ttcgtgattc tttagcggat atttcaaaga tccgttcttt aggttatcaa
    17701 cccaaatttg atatattgaa cggaatggaa cgttatttaa agacagaaat cgattgaaac
    17761 aaaaaaccag catcctgctg gtttttttgt ctcgacttag ctttttaaat taacaccttc
    17821 catcagttcg taaatctgat ttcttaaatc agtggccggc aatttgatgt ttttatcaga
    17881 ttggatatta ctttccatga cgattgcatc ttttgtgtct tttttcatcg ccgctactgc
    17941 atggaatcga ttgatatttc cctgagcaag gatataatca tttccactgt aaacaccgcc
    18001 gctgtaagga tgtttatcac cattattttg ccacaatgag aaaagtagtt cttttggtat
    18061 aatatcgcca gacagaacac tggtaaagaa tttgttcaag tcataaacag acatacttat
    18121 atttcctgtg cctaattcat ttcggatgtc tgtttcaggc tcgaccaatg cttttgaata
    18181 atcgtcagtt gcagacattt ggtaagatac agcatgctga ggggaatttt ttacattttc
    18241 ataaaaatct gtatgttcta gatggagtgg ccgaatgatt tcttttttta aaagagtttc
    18301 ataaggctga ttcattagtt tgatcaatac ccctgtaagc aatgtataat ttaatggttc
    18361 ataatgatac gtatcataac cagtaaattt caaatgatga agggcaaact gaatgatctc
    18421 actatctgac ataggttttt ttggagaagc ggttcgatga agaccagagc gcatgtaaag
    18481 catatttttg attgagattt gctgacttcc aggaatctga ggataaaatt cagatagctt
    18541 tgtgtcaaaa gttatcttgc cagattgaat ttgttttagg ataagtaaag cagtcatcgc
    18601 tttttggatc gaaccgattt gatagtaaag agaaggagaa ttcgattctt ttatgcgtag
    18661 atttgcgtac ccatatcctt tttctaatat cactcgatta tttttgcgta cataaatcga
    18721 accgatataa tctgattttt gcagtaattg atcaattttg ctggcaagca tctgctcatt
    18781 agatgttaaa ttggaagcaa gttgtgcggg ttccgtttta taaatttgtg gttgaggttg
    18841 ttttgttaat tgccatcctg taatgatgcc tgttgaaaag aagctgacag ccagcaacaa
    18901 aactgcgaga aaagaaagag agatctgctg ttttttttga tgttttcctt ttgccatatt
    18961 aaatcctttc tatccatttt taactatttt tcattgtaat cctcaaaaag agcatttgcc
    19021 acttacaatt ttgacataat atttggtaaa ataattcacg gcagtagaaa tttcatgtat
    19081 aataaacaga gttacgaaga tgatagtaag gaggagttgt tttgtcagga aacaaacaac
    19141 acccaagcaa agacaaaaaa gaagccagta aggcagctac agcagatata atcgacaagc
    19201 ggagaaatct gcgtaaaaaa gaagataaga tcgtgaggaa gatcatttta gtgatcgctc
    19261 ttacattatt gattattggc ggatttctcg ggtttaccgt atatcgttat gtagatagcg
    19321 ggttgaagcc tttagataaa tcggacgacc aattagtcca agtggaaatc ccaaacggct
    19381 cttctaataa gcagattggc gaaattttgg agaaagacaa tattattaaa agtgggattg
    19441 ttttcaatta ttatacaaaa tttaaaaact taactggctt tcaagctggt tattatcagt
    19501 tagcgcctaa tatgactctt gatgagattg gcaagcagtt gcaagaaggc ggcacatctg
    19561 agccaacaaa agttgcagat gggaaaattg ccattccaga aggctacgac atcgatcaga
    19621 ttgctgagcg ggtagcgaag gtaacaggta aagacaagaa agaattttta gatttagtaa
    19681 atgatgagac tttctttaac gagttgtatc aaaaattccc agaattgttg gaaagtgcct
    19741 ctaaagctga gaacacaaaa taccgtttgg aaggctactt attcccagct acttatgatt
    19801 actacacgag tacttcgctt aaggatcttg tgattgagat ggtgaacaag acaaatacgg
    19861 tcatgcaaaa ctattattca gctatcaaac agaaaaattt gactgtacaa gaagttttaa
    19921 ctttggcttc tctagttgaa aaagaaggcg taaaagaaaa tgatcgtaaa aatattgcac
    19981 aagtcttctt taaccgaatc aaggcaaata tgccacttca gtcagatatc tctgttttat
    20041 atgcattagg agaacataaa gaattggtga cgtatgaaga tacagctgta gattcaccat
    20101 acaatttgta taccaatacc ggctatgggc ctggtccttt tgacaatcca agcgaaaagg
    20161 caatcaaggc agtattagaa ccagcagaaa atgactatta ttatttcgta gcagatacga
    20221 gcactggaaa tgtttatttc gctaaaacgt atgaagaaca tatggaacta gtgcaaaaat
    20281 atgtaaataa ttcttagaaa aacaggggct agtcattgat catttttaga aaaacattta
    20341 cattttaaga aaagcgtggt aatctttttt ggactatatt cctcaaaaga ttaccttttg
    20401 cggtatttta tcgaaaaaag gagacgttac acatggtaga gaaagtttat ccaatgactc
    20461 tagaaggtaa agaaaaatta gaaaaagaat tagaagaact aaaaacagtt aaacgcggag
    20521 aaatcattga acgaatcaaa atcgctcgcg gtttcggaga tctttccgaa aactccgaat
    20581 acgaatcagc taaagatgaa caagcatttg ttgaaggaag aatcacaaca attgaaaata
    20641 tgatccgttt tgcacaaatc atcgataatg acggcgtaga tgcagatgag gtatcgatcg
    20701 gtaaaacagt tacgttcatc gagttgcctg atggagatga agaagagtac acgattgtcg
    20761 gaagtgctga agcagacccg ttttctggaa agatttccaa tgattctcct atcgcgcaag
    20821 cattgattgg caaacgtaaa aatgatgaag taaccattgc tacaccaggt ggcgatatgt
    20881 tagttaaaat cgtcaaagta gaacaagcgt agtctgactg aaagatctag agagttcatc
    20941 ttactgatga cgcactctag atcttttctc tttgagtatt gcaggtttca ctgaacgatc
    21001 ttacatctaa agtcctatag ccaacagtca aaaaaatagg tatgatgaaa tgagaatgtg
    21061 tcaggaggga gaaaaagcga tgaatagttc ttggcgtttt tttgtggtag tcgaagcact
    21121 gctgctgtta tttgctgttt ggcaaatcgt aaacaatact ggacttttgc ttttagtaat
    21181 attcggtatt ttcaatatat atctagctat gcgcaaatat cctcgtacta aatttcaaaa
    21241 ttttcaattg atcttaggga gcttggtcat tttttttagc ttagtcaaca gtcctgcttt
    21301 atggatgatg gttgtattgg ccgttttatt tatcgggctg aaaggtgtag agatttcagg
    21361 aatagatttg acaaaaaatg ctttttggcg aaaaaaacag atcatgatgg tccaaacaga
    21421 gcaattaaaa acacataata atgagcgtaa aagacagcaa ttatttggta accaacgtat
    21481 tggaaacgac gtctatgaat gggatgatat caacattgcc atcatttctg gtgatactat
    21541 catcgatttg ggtaatacac ttttgccaaa agatgataat attgtgattg taagaaaagg
    21601 gataggcaga acacggatct tagtacccct gggagtggct attcgtttag agcatgcgac
    21661 attagtagga aatgtcttgt ttgaagaaga gcagttttcc ttaaaaaacg aacagatcaa
    21721 aatctgcagc agtgattatg atgaaaatcc tcgcagattg aagatcatta ccaatacctt
    21781 ggttggagat gtcgaggtga tacgaatatg atgggaagaa tatccaaagc aatgctagct
    21841 gtttattcgg ggattgcggc tttccttatt attctatttt cacttttcac ttatttttat
    21901 gccagtaacc aaagtcattg gtggggagaa ttgctgcgtg cgcgtttatt atacgttccg
    21961 cttatcttcc atttgatggc aatttcatta ggtgtaggat tgatcgtatt tctattgtta
    22021 tcacttattc aaaaggcaaa atacgggaaa atcgaagaaa agcttcgtgc actttcttct
    22081 ggcaattatg aatccaaact tttgcatcag ccgatcccaa gcgcgtcgga tgatctgtac
    22141 atcaaagata ttgatatgga aattgctaaa ataaaagaaa aaatgatcga agtatccagt
    22201 gaattacaga ttatgactag ccgtcctcaa tatgtagatg gacaaaccaa agaagagatt
    22261 ttagaattag aaaggcatcg attagctcgt gagcttcatg attcagtttc acagcaatta
    22321 tttgcagcga tgatgatgat gtcggcattg acggagcaag cagaaaaaag tgagacacca
    22381 gaaatgttcc gtaagcagtt gaaaatggtg gcagagatca tcaacgcttc ccagtctgag
    22441 atgcgtgcgc tgcttcttca tctgcgtcca gtcaatttag aagaaaaaag tttaaaacaa
    22501 ggcattgagc agttattgaa ggaattgcag aataaaatcc agatttcgct aaaatgggat
    22561 gtagaagatg taaaattaac tagttccatt gaggatcact tgttccgaat cgttcaagaa
    22621 ttgttatcaa acacattaag acatgctaag gctaatgaat tagaagtcta tttgcataaa
    22681 atagataaca atcttttatt acgtatcatt gacgacggga caggatttaa tatgaatgaa
    22741 accaaaacgg gaagttatgg attgaacaat atcaaagaac gagtagctgg tattggtgga
    22801 acagtaaaaa tcattagttt taaagggcaa ggtacgagcg tggagatcaa ggtccctttg
    22861 atgaaggagg catagataaa gatgataaaa gttttattag tagatgacca tgaaatggta
    22921 cgcttaggcg tctcttctta tttatctatc caagaagaca ttgaagtgat tggagaagct
    22981 gaaaatggac gtcaaggcta tgaaaaggcg atggcacttc gaccagatgt cattttaatg
    23041 gatctagtca tggaagaaat ggatggcatc gaatcaacaa aagcaatctt aaaagattgt
    23101 ccaaaagcca aaatcattat cgtcacaagt tttattgatg atgaaaaagt ttatcctgca
    23161 attgaagcag gagcagcagg atatttgttg aagacgtcta ccgcacatga aatagctgat
    23221 gctataaggg ctacgcaacg cggcgagcga gtattggaac ctgaagtgac aacaaaaatg
    23281 atggaaaaaa tgagtcggcg aaatgatcca gtattgcatg aagaattaac gaatcgagaa
    23341 aatgaaattt tgatgctgat ttctgaaggg aaaagtaatc aggaaatcgc ggatgaattg
    23401 tttatcacac tgaaaacagt taaaacgcat gtgtcgaata tattagctaa gttagaggta
    23461 gaagaccgaa ctcaagcagc tatctatgcg tttaaacatg gtttggtgaa ataaaaacga
    23521 tgataaagag gataatcaaa agctgagaaa ataactatta tttttcttgg cttttttaca
    23581 ataatgatgc ttgattttgt ttttggaaag gctttgttat actgagaaaa gaagatacta
    23641 aaggaggaaa atggtgaaac aaaattttgc aattgtaggc cttggacgat ttggcggaag
    23701 catctgccgt acactgatcg aagctggaca agaagtactg gctattgaca gtaatgaaga
    23761 tcgagtcaat gaatatatga atatagccac tcatgctgtt gtggcaaatg cacaggatga
    23821 gatgacactt cgttcattag gtataagaaa tttcgatcat gtaattgtcg ctattggaga
    23881 agatatccaa gccagtatct tagttactct gatggtaaag gaaatgggcg taccgaatat
    23941 tttagcaaaa gcccaaaatg agtaccatgc ccgtgtacta gagaagatcg gagcagatcg
    24001 tgtcgtacac cctgagcgag acatggggat acggattgca cataacttag tatcaaagaa
    24061 tattctagac tacttagagc tgtcagataa atattcttta gcagaaatcc gagtctccaa
    24121 tcctaaattt ttcaacaaaa cattagctga attaaatttc aggcaacgtt ttgacttaac
    24181 agtagtcgcc atacgaagag tagatcaaac ggttatcgct tctcctgcag cagatgaata
    24241 tgttcgcgaa aatgataatc tactggtgat tggagagaca ggcgatgtgg atattctaga
    24301 cgataagatg aatcaataga aagaaaagag aaagtaggtt gttgggatgg aattaacggt
    24361 aacgccaaaa gcacaaaagt ggtttgactc agaaattgat ctgccagaaa cttacggaat
    24421 acgttttttt ggaaaagtct acggcaaaac agaggttcat gatggttttt cgattggaat
    24481 gtcagttgaa cagccagaac atcctgtaaa gaaagtggaa atggatggga cacttttttt
    24541 tatcgaggaa gcagatgaat ggttctttaa aggatatgat ttacttgttg attatgatcc
    24601 caacctggag gagccaacct atcactttaa aaaacaataa aaaaagagag ggtgacacaa
    24661 gtttt
//
LOCUS       DACEQH010000042        23657 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.042, whole genome shotgun sequence.
ACCESSION   DACEQH010000042 DACEQH010000000
VERSION     DACEQH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.042"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            255..530
                     /locus_tag="IW833_001773"
     CDS             255..530
                     /locus_tag="IW833_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307891.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            543..2621
                     /locus_tag="IW833_001774"
     CDS             543..2621
                     /locus_tag="IW833_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1307892.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2781..4667
                     /locus_tag="IW833_001775"
     CDS             2781..4667
                     /locus_tag="IW833_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1307893.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="IW833_001776"
     CDS             4679..5626
                     /locus_tag="IW833_001776"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1307894.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="IW833_001777"
     CDS             5646..6173
                     /locus_tag="IW833_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1307895.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="IW833_001778"
     CDS             complement(6236..6889)
                     /locus_tag="IW833_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1307896.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="IW833_001779"
     CDS             7021..7863
                     /locus_tag="IW833_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1307897.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="IW833_001780"
     CDS             8021..8917
                     /locus_tag="IW833_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1307898.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="IW833_001781"
     CDS             8920..9633
                     /locus_tag="IW833_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1307899.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="IW833_001782"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="IW833_001782"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1307900.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="IW833_001783"
     CDS             10168..10395
                     /locus_tag="IW833_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1307901.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="IW833_001784"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="IW833_001784"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1307902.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="IW833_001785"
     CDS             complement(11181..11723)
                     /locus_tag="IW833_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1307903.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="IW833_001786"
     CDS             complement(11960..12358)
                     /locus_tag="IW833_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1307904.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="IW833_001787"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="IW833_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1307905.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="IW833_001788"
     CDS             13471..14238
                     /locus_tag="IW833_001788"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1307906.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="IW833_001789"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="IW833_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1307907.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="IW833_001790"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="IW833_001790"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1307908.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="IW833_001791"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="IW833_001791"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1307909.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18894..19799
                     /gene="xerC"
                     /locus_tag="IW833_001792"
     CDS             18894..19799
                     /gene="xerC"
                     /locus_tag="IW833_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1307910.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19836..20384
                     /gene="hslV"
                     /locus_tag="IW833_001793"
     CDS             19836..20384
                     /gene="hslV"
                     /locus_tag="IW833_001793"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1307911.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20398..21798
                     /gene="hslU"
                     /locus_tag="IW833_001794"
     CDS             20398..21798
                     /gene="hslU"
                     /locus_tag="IW833_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1307912.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21816..22607
                     /gene="codY"
                     /locus_tag="IW833_001795"
     CDS             21816..22607
                     /gene="codY"
                     /locus_tag="IW833_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1307913.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22689..23564
                     /locus_tag="IW833_001796"
     CDS             22689..23564
                     /locus_tag="IW833_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1307914.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8192 a         4079 c         4983 g         6403 t
ORIGIN      
        1 tttgtcgcag ccccattttt ttgttgtgat aaaagaacaa gaaaggcagg aatgggaaag
       61 taaacatcct tttgtcagaa acttttatct ttcttaagtt ttacttgata tctgatacta
      121 tttggaatag aatgaggcgt tgaaaaattt atggaaattt ctaactagca ataaacgatg
      181 aaacaatgaa atatttttca tccaaaaaga actttgctat aatgatggaa gtttgtgtat
      241 aggagatctg gaaaatgaga gagagttttg aacaacaaaa aaaactgttg catgatcgct
      301 atggcgccct ttcgatggat gaccgccgtc aaatactttg taagttgcgt aaaagaaata
      361 tattgatgta tcgtcagttg gaacgtctga aacatgatct gcttcgtttg gagtcaaaac
      421 gtgtacagtg tgaattagag gggaaccaaa cacaggttga agtagtcgaa accaaaattt
      481 taaagaaaaa agaacaattt ttaaaaatgc ttacacaaaa taaaaaataa aggggatgga
      541 cgatggaatt tgctgagtta gttattgtat ttgcagtaac gatcacctta tcgaacatag
      601 ctagccgcat tttgccaatg attccagctc cactgatcca aattttcctt ggagtggtat
      661 taggtctgac agagtgggga caatcaatcg attttgaacc tgaattgttc ttagttatga
      721 ttattgcacc attgttattt agagaaggag aaaaagcaga tatttcttcg attctcaaaa
      781 atttcgggac aattttgttc ttggcatttg gaggagtcat tttgaccctt gtcggggtag
      841 gtgcgacgtt atcattcctg ttgccaagtg tcccacttgc tgcctgcttt gcttttggag
      901 cagcgttagg tccgacagat gcggtagcag tcagttctct ttctggacga gtgaatatcc
      961 ctaaaaaggc gatgcatatt ttggaaggag aaggtctttt gaatgatgca tctggtgtta
     1021 ctgccttcca gtttgctctt ggtgcgctga tcaccggatc tttttcagca gtaaacgcgg
     1081 ggatgagtct agtagtttct agtattgggg gagctttgat cggctttttg cttgtctggt
     1141 tcaaacaaaa aataattcat ttgatcgaaa aagcatcagc acaagatgtc acagggtatc
     1201 tgctgatcga acttttgctt ccttttctag cctatgtatt ggcagaggca tttgatgtat
     1261 ctggaatcat tgctgcagtg gcagcaggta ttctccaagc ttctggtttt aggaaaatct
     1321 ctgtgtttga tgcagaattg tcgagcttgt cccacagtac atggacaact attgctttta
     1381 cactgaatgc attagtattt atatttttag gaatcgaatt gacgcaagtg ttctcgccag
     1441 tatggggaga cggtttatat ccaaacggtc ttttattagc gattatcgta ttaatctctg
     1501 tcatgctttt tgtgatcaga ttcatttcta tcagtctttt ttatgtattc aaagatggca
     1561 gtaaaaagtt caaaaaacag ttgaacgaga tcttgatctt aacatttggc ggtgtaaagg
     1621 gaacagtcag tttggctacg atttttattt tgccattttc aatcaataat atgatgtttt
     1681 accagcggtc tttgcttttg tttctgacgg cgggtgtcat cttagtgact ttggtcatag
     1741 ggatcattgt cttgcctatg ctgacggaaa cagaggaagc gaaaagcacg gatttgaatg
     1801 ctttgatgat tttagaagag gtcgttgaag ttttgcgaaa agaaataaag gaaattgatc
     1861 gtaatacaaa agaattttta gccactgaag cagtgataga aaattatcaa gaacggatac
     1921 gtgatctgta cttggaagat ttaaccgatg atgaaaagca agaagtacaa gaaatacaag
     1981 cactgatttt gtcgatcgaa cgggatggat tggatgaaag ttatcgttcg ggcaagcttt
     2041 cttctaatgg ttatcgtttt tactcccgtt ttctttctcg attcgaacat tctatcacaa
     2101 gccagatttt gtcttttatc ggcttttggt tcattgttgt ccgtaggtta atgcgaatcg
     2161 ttctccatcc gaaaatgttt tggcaaagaa gatatgctga tcgtcagaca tttattagtg
     2221 atgaagatat ccaagaaatc agagagacat atcaaaaaaa tactcagttg attattgaaa
     2281 gtttagataa cttgactgat atttatgatg aaacgatggt tcaatttttt atccagcaac
     2341 gcaaacttga aggaataaaa atgaactcgg ggaatttgat ttcttcgtgg atgatccagc
     2401 aagatagtct gtttacgaaa aaaatgctaa gaggttatta tcttgaaaga aaaatcattg
     2461 atgaatacga agttgctgaa aaaatcacga cgttttctgc gaacagctat cgcaggaata
     2521 tcaatttgtt agaatcttat acgatgaaca aaccatctga taacttttct ttccgatttg
     2581 cgtttcgagc aaagtcaaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcattttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacaccggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt cttgcgtttt tcttaagaat ttctcaaaca aaatgtgagg
    18781 aattgcgcca attgtgaaca aacttagaaa tattggttaa attctgacaa tttatttgtc
    18841 tttttataaa cgctgtgcta aattattctc tgggacaaaa ggaaggtgag aaaatgacag
    18901 aagccacgtg ggaacaaaaa tttttgcgtt atttgattgt cgaaagagga tattcagaaa
    18961 aaacgaaaga agcatatgaa gaagacattc tccatttcaa acattttttg gaggaatctg
    19021 gagacgctga tctgctgaag gcggaatatt ttacggttcg tgtctatttg agtgcattag
    19081 ctgatcatcg tcctagctat agcagaaatt ctatcagtcg caagatagcc agcctacgtt
    19141 cgttttatca gtatcttctc aaagaagaag tgataaaaga aaatccattt tcttacgttc
    19201 atctcaaaaa gaaaaatttg agactgccac gtttttttta tgaaaatgaa atgcaagcac
    19261 tttttgacag cgtagcagga gatagtcctt tggatttgag gaaccgcgct ttactggaag
    19321 tcctttacgg gtcaggcatc cgtttaagtg agtgtagcag tttgtcggta tcagacattg
    19381 attttgattc agaggtcatg ttgatccatg gtaaagggaa caaagagaga tatgctcctc
    19441 taggttcttt tgctcaagat gctctgcaag aatatttcag ggaaggccgc caggtattga
    19501 tgacaaaata tcacgaagag catgatatcg tttttgtgaa tcatcatgga aaaccaatca
    19561 cgcctactgg aattgaatat gtcttgaatc aaatgatcaa aaaaagcagt ttggatagtg
    19621 atatccatcc tcacatgctg cgccatacat ttgctactca tttattaaat aatggggcag
    19681 atttgcggac agtccaagaa ttgctaggac atgccaattt atcgactacc cagatctatg
    19741 cccatgtaac aaaagaaagt ttacaaaaaa actatcgttc tttccatcca agagcgtaaa
    19801 aataaggaaa agaacaaatt taggaggaaa caaacatggt cgaatcacca tttcattcaa
    19861 ccactatctg tgccgtcgaa aaagacggga aatttgctat ggctggagat ggccaagtaa
    19921 caatgggtga atctgttgtc atgaaaggca ctgcaaagaa agtacgacgt atctacaatg
    19981 gagaagttgt tgtaggtttc gccggaagtg ttgcagatgc gtttacttta gaagaaaaat
    20041 ttgaaggcaa actaaacgaa tataacggga atctacaacg cgcagctgtc gaattagctc
    20101 aagaatggcg gacacagcaa tctatgcaaa aacttgaagc gatgctgatc gtaatgaatg
    20161 ataaagaaat gcttcttgtt tcaggtacag gagaagttat cacaccagat gatggtatct
    20221 tagctatcgg atcaggaggg aattttgcgt tggctgcagc ccgagcaatg aaaaactatg
    20281 ctcaaaacga catgagtgcg aaagaaattg cagaaaatgc gttaaatatt gccgcagata
    20341 tctgtatctt tactaatcat aatattatcg tagaagaact gtaagaggtg agtagatatg
    20401 tatgaattaa aacaaacacc aagacaaatc gtaagtgaat tagatgaata tatcgtagga
    20461 cagcacgcag caaaaaaatc tgttgccgtt gctttgcgta accgctatcg ccgtttgcag
    20521 ttagatgaaa aaatgcaaca agatattaca ccaaaaaata tgctgatgat cggacctaca
    20581 ggggtcggta aaacagaaat cgcacgacgt ttggcaaaaa tcgtcaatgc accatttgtc
    20641 aaagtcgaag caacaaaatt tactgaagtc ggttatgtcg gaagagatgt agaatcaatg
    20701 gttcgggatt tagtagaaaa tgcgatccaa atcgtagaaa aagagcagta cagccgtgta
    20761 tacacaagtg ctaaaaaaaa tgcagaaaaa cgtttggtaa aaatcttggt acctggtatc
    20821 aaaaaagaac agaaacaatc cactaatcaa tttgaacaaa tgatgaatct ttttaatgct
    20881 agtcagcaaa ataccgctga accgcaagaa gaagtgacag aagatattcg aatcaatcgc
    20941 cgtacagtct tggaacaact acaaaagggt ttactggata gtcgtgaagt aaccattgaa
    21001 gttgaagacc aaaagaaacc tgcaccaatg atgaacaatg gattggagca aatggggatc
    21061 gacctaaatg aaactctagg tgctttgaca ccgaagaaaa aagtggaacg gacggtaaca
    21121 gtcaaagaag ctttagagtt attgatcaag gaagaatcag caaaattagt caaggatgct
    21181 gatatccata gtgaggcaat caaattagcg gaagaaagtg gaattatttt tatcgatgaa
    21241 atcgataaga tcacttctaa atcacagcaa tctggagaag tatccagaga aggcgtccaa
    21301 agagacatat tgccaattgt cgaaggttca caagtaaaca caaaatacgg gatcatccaa
    21361 acagatcacg tcctattcat tgcttcagga gctttccatt tgtctaaacc aagtgatcta
    21421 attccagaac ttcaaggacg cttcccgata cgggtagaat tagatgattt gacagctgaa
    21481 gattttgtga agattctgac cgaaccaaat aatgctttag taaaacaata cgtcgcgttg
    21541 atcggtacag aaaatgtgtc agttacgttt acaaaagaag caattgaacg gatcgctcaa
    21601 atcgctttcg atgtcaaccg tgacacagat aatatcggcg cacgcagact gcataccatt
    21661 ttggaaaaac tactagaaga tctattattt gaagcaccag atatgcaaat gggagaaata
    21721 acgatcacag aagcatatgt ggatgacaaa cttggctcaa ttgcggcaaa tgaagattta
    21781 agtcgctata ttttataaaa aatcggagga cgacaatgac agacttgcta acgaaaaccc
    21841 gcaagataaa cacacttttg cagcaaaaga atacttttga catgcaagca gatttgcctt
    21901 acgataaaat ggctgtcaca ctaggtggta ttcttgacag caacacctat atcataggaa
    21961 gtgacggagt attactaggt tataatgaac gtcacgatgt gaacaatgaa cgaatcaaaa
    22021 caatgtttgt tgagaaacaa tttcctgaat cttatactga tacagtggca cagttgatga
    22081 aaactgaagc taatatccca attactagtg atattactgc atttccggta gagtcaagag
    22141 agaaatatcc atttggtctg acgactgtcg ttccgatctt tggagctggc gaacgtttgg
    22201 gtacgattat cttatctcgt atggaacaat cgtttgcaga tgacgatcta gtcttagcag
    22261 aatatggagc tacggttgtc ggtatgcaga tcctttatca gaagtctcga agcatcgaag
    22321 cagatgtacg gagtgcaacg gctgtccaaa tggcaatcaa cacattatct tatagcgaat
    22381 tgaaggctgt acaagctatc tttgaagcat tggatggaga tgaaggtcgt ctgactgctt
    22441 ctaatattgc cgatagtatc ggcatcactc gttctgtgat cgtcaatgct ttacgaaaat
    22501 tggaatcagc tggaatcatt gaatctcgtt cgttaggcat gaagggaact tatttaaaag
    22561 tattgaattc ccgttttaaa gacgagttag caaaacacag ttattagaag taaggggact
    22621 ggggcagata gaaatgtttg ttccagcctt cccattttaa aaagtaagtc gaagttggag
    22681 gaatggcaat gactgttatt attcaaaatg accagctgat cgccgaaatc gctgaacacg
    22741 gtgcggaatt gatcagtctg aaatcaaaag aacatggttt agagtatatc tggcaaggtg
    22801 atccagcgta ttggggcaga catgcaccgg ttttattccc ttttgttgga cgtttaaaag
    22861 atgatcagta tgtttatcaa gggaaaacat atcaaatggg gcaacacgga tttgctcggg
    22921 atatggattt tgaagtcatt gaacatggac aagaaatggc tagtttcatc ttaaggagta
    22981 cgccggagac attagaaaag tatccatttg attttgaatt agtgatcagc tatgaattag
    23041 gaggagacgg catcacggtt cattatcaag tggcaaatac cggtgaaaaa gaaatgttct
    23101 tttctatcgg tggacatcct gctttcaatg tacctcttga agaaggattg tcatttgaag
    23161 atttctatct ggcattttct ccattgaaat caagattacg cctaccattg aaaggcccat
    23221 tcatcgatat ggatcaaaag acgttaggtc agacaaatac gaatctggct ttgaatcgtg
    23281 aactttttga gcaagatgcc ttggtttttg aaacaagggg tctaaatgcg tacgccattc
    23341 gaagtgaaaa atcttcccat agcgtaactt taagctataa agatatgcct tatgtaggga
    23401 tctggtctcc ttatccaaaa gaagcgcctt tcgtttgtat cgaaccgtgg tgcggaatcg
    23461 ctgacacggt gaacagtagt ggaaacttga tggaaaaagt agggatcaat caactaggtg
    23521 cccatgaaat cttcaagaca aaatattcca tcacagtgaa gtaagaggtt gtgaccgaga
    23581 agttagcagg gaacaccgtt taccaaggtg tgtaaaaaag ctatcctgta agtaataagg
    23641 gagtgtgaca tcgattt
//
LOCUS       DACEQH010000043        23397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.043, whole genome shotgun sequence.
ACCESSION   DACEQH010000043 DACEQH010000000
VERSION     DACEQH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.043"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            144..542
                     /locus_tag="IW833_001797"
     CDS             144..542
                     /locus_tag="IW833_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1307915.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1221
                     /locus_tag="IW833_001798"
     CDS             526..1221
                     /locus_tag="IW833_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ1307916.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1805..2692)
                     /locus_tag="IW833_001799"
     CDS             complement(1805..2692)
                     /locus_tag="IW833_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ1307917.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2877..3716
                     /locus_tag="IW833_001800"
     CDS             2877..3716
                     /locus_tag="IW833_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1307918.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3912..4886
                     /gene="sgrA"
                     /locus_tag="IW833_001801"
     CDS             3912..4886
                     /gene="sgrA"
                     /locus_tag="IW833_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ1307919.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5184..5540
                     /locus_tag="IW833_001802"
     CDS             5184..5540
                     /locus_tag="IW833_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307920.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5543..6700
                     /locus_tag="IW833_001803"
     CDS             5543..6700
                     /locus_tag="IW833_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307921.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6726..7166)
                     /locus_tag="IW833_001804"
     CDS             complement(6726..7166)
                     /locus_tag="IW833_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1307922.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7326..7832
                     /locus_tag="IW833_001805"
     CDS             7326..7832
                     /locus_tag="IW833_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1307923.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            8000..9913
                     /locus_tag="IW833_001806"
     CDS             8000..9913
                     /locus_tag="IW833_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1307924.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9918..10919
                     /locus_tag="IW833_001807"
     CDS             9918..10919
                     /locus_tag="IW833_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1307925.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11121..11327)
                     /locus_tag="IW833_001808"
     CDS             complement(11121..11327)
                     /locus_tag="IW833_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ1307926.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11973..12821)
                     /locus_tag="IW833_001809"
     CDS             complement(11973..12821)
                     /locus_tag="IW833_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1307927.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12985..13887
                     /gene="gnd"
                     /locus_tag="IW833_001810"
     CDS             12985..13887
                     /gene="gnd"
                     /locus_tag="IW833_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ1307928.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13889..14890
                     /locus_tag="IW833_001811"
     CDS             13889..14890
                     /locus_tag="IW833_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1307929.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14887..15171
                     /locus_tag="IW833_001812"
     CDS             14887..15171
                     /locus_tag="IW833_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1307930.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15204..16001
                     /locus_tag="IW833_001813"
     CDS             15204..16001
                     /locus_tag="IW833_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1307931.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            16015..16848
                     /locus_tag="IW833_001814"
     CDS             16015..16848
                     /locus_tag="IW833_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1307932.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16866..17723
                     /locus_tag="IW833_001815"
     CDS             16866..17723
                     /locus_tag="IW833_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1307933.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17745..18254
                     /locus_tag="IW833_001816"
     CDS             17745..18254
                     /locus_tag="IW833_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1307934.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18291..18692
                     /locus_tag="IW833_001817"
     CDS             18291..18692
                     /locus_tag="IW833_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307935.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18804..19157
                     /locus_tag="IW833_001818"
     CDS             18804..19157
                     /locus_tag="IW833_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307936.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19191..19634
                     /locus_tag="IW833_001819"
     CDS             19191..19634
                     /locus_tag="IW833_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1307937.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19651..20736
                     /gene="uxuA"
                     /locus_tag="IW833_001820"
     CDS             19651..20736
                     /gene="uxuA"
                     /locus_tag="IW833_001820"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1307938.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20729..21442
                     /locus_tag="IW833_001821"
     CDS             20729..21442
                     /locus_tag="IW833_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1307939.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            21771..23072
                     /locus_tag="IW833_001822"
     CDS             21771..23072
                     /locus_tag="IW833_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1307940.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         8021 a         3676 c         4819 g         6881 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata
     1081 acgttaggta aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg
     1141 aggggcttcc aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa
     1201 aaggtcgctg attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa
     1261 actcgaggag gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac
     1321 ttatttctca tttaaacgat gtattcccta acctctaata catccttgta actctgaagt
     1381 tacgccagtt ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata aaaatatctt
     1441 tcgtgtttct ctagtataac agaataagag gaacaagtcc agaagggata tttttagtct
     1501 tttttaaaat aacttttcag tcgagtttga gtttatgata gttgtttttg aagcgatacg
     1561 gcaagtgaaa aaatagggaa gcatgaaaga atctacttaa tggcgcattg attcatgtga
     1621 tagaaataaa aaactttttt aaattatatc ctcatgattg taacaaatcc agtgatttgt
     1681 tttggtacaa ataaaaaaag gccccgatga agttgtggca aaagtggttt gcctcaaaaa
     1741 taagaaggaa tttccaaaaa tcacttctca aattttgcca catccccaat tgggtcaaat
     1801 ttgtttattt gaatatattt tttacataag gatctttaat agttacattg tcttttttca
     1861 tcactttacg gattgtttta tccatgaatt ccgtatcaag agtttttgct tcggtagcaa
     1921 ttttttctag ctcagatttg tatttgtctt tattagaacc tttatcctgt ttttttacca
     1981 tttttactag ataatagctt tgctgataag tcgttggatc tgttacagga atgatatcag
     2041 aaacttcccc gttttttagt ttaaatgctg ctttttttac ttcagtagga atctctggat
     2101 tggttgaatc aaaattcatt tttccatcag tttcctttaa tgttttattt tttccataag
     2161 cttgaacaag cttggagaaa ttttcccctt catttgcttc tttttttgct ttatctgctt
     2221 cttctttagt cgaaaataga atgatctgtg tactaacttc tggatagaat tctttccaag
     2281 cagtgtctag atctttatct gttaatttta cattggcttt caaaccgtat tggaaagcca
     2341 gcatctgttt aaagagtttc ttatatgtct cttctgtttg gccaactgct tttaattgtt
     2401 ccttgtagtt tggaccgagt tttttgattt gttcattata ttcttttgtt acttcctttg
     2461 aagaaatctc ttttccgtat acatcatcaa atgctttgaa gacaatcatt ttttttaata
     2521 gtgtttgatt atttgattta acttttgggt tatcataaaa atcctgaact gtaagtgttc
     2581 cacctttaaa cgttgcaatc gtttcattgg acttgctaca acctgagaat agtaaaattg
     2641 cacatgctga aaggcacata attagtagtt gttttttcaa aaaatttccc atcctttcct
     2701 tattaaaaac agtctaacag aagaattagg gagacagtct ttcattttcg taaggttgct
     2761 gtagatattc ttagttcgta aaaatctatt ctctctatga tggagaacat ttacatagtt
     2821 ttcacgagac aagaaagaaa agggttgtta aacttaatat aaaggaggga tgaaagatga
     2881 tcaggattat tcggttgatt ggatctgttt taatagtctt agttggtttt gttttaggaa
     2941 ttcttgtcaa taatgcactt gcggatatgt ttattggaaa tagtgaatgg attggagcaa
     3001 ttgccggtac ggtcggacat ttagctgttt ttttactagc tttatttctt gctagtaagg
     3061 tcgaaggaaa aagaatagaa gattatggtg tttctgttcg aggaaaagac tgggggtatt
     3121 tagcgggagg tctcgttgtt ggaattggag tgtttctgct tattactagt ccattatatt
     3181 tgacgggggc gtatcggtta gattcaggaa acgcaaacat tgtgccgctg ataacgagtt
     3241 ttattttatt tattgcagtg ggggcatcag aagagctttt attccgtggc ttttttcagc
     3301 atcagatgct tagatttggt ccgttaatag caatgattgg atctgctgca ttatttgctc
     3361 ttttgcacgg attgaatcct aacatgacat ttctggcagt tttcaatatc tttttagcag
     3421 gttgcttttt cagcgcactt atctacagta caggaagttt atttacggct attggtgctc
     3481 atattacttg gaattggaca caaggagcta ttttgggtat accagtcagc ggaacgcaag
     3541 aacaaggttt tttctccact ctgatccaat cgcaaaatac aataattact gggggaaact
     3601 ttggggctga agcatctata tcaactacat tattcttgtt agtcttgatt gttggtttgt
     3661 tactctatgc atataaaaca aagcggatga agaaatacac tgctgtttat caataaaaga
     3721 tgaagaaagc aaggctgata tcggtcttgc ttttttaagt ctgtcattga ttaaacgaaa
     3781 aagtaaacaa attcaagcga atacacgaca tcttttgtta gattttcaca actgactgtc
     3841 gtgttctcat cattttcatt ttatataatt cctttatcaa atagaaggta attatatgga
     3901 gagtgataga aatgaagaaa acagcgaccg tattatttgt tattcttggg ctgagtataa
     3961 ttgcagcaat gattggaatc agtacttctg caaatgaacg ggcaaatgag aatctttcat
     4021 ttactgtgaa aactgatcgg attgtttatg acatgacaca acaagtcatt actattccag
     4081 tcaaaccaaa caaaagcgtg aatgctagtg atgtacatgc tgttctaaca tatggttggg
     4141 atggtaatgg aagttcagaa aaagttattg gggaagttta tttaaaagac gtacaatgga
     4201 ccgctggtat tgagtataca ataatgattt ctgcagaact atcgattgat gagatcaaga
     4261 gtaaagataa agttgatttg attgtttttt atgatggaca gatgacgatc acggagaatt
     4321 tgaaaccaag cagttggaca gttgttggac cgccaagcag tacggatagc acaagtagtg
     4381 aaagcagtac agaaaacacg agcggtgaaa gcagtacaga gagtacgagc agtgaaagta
     4441 gcacagaaaa cacgagcagc gaaagcagta cggagagtac gagtagtgaa agtagcacag
     4501 gaagcacgag cagcgaaagc agtacggaga gtacgagtag tgaaagtagc acaggaagca
     4561 tgagtagtga gagtagcaca ggaagcacga gcagcgaaag cagcacagga aaagtaaata
     4621 atgaacttga tttggtacct atcataccaa ctaaggaaca aaagagaaat gcagggagca
     4681 acctaaaaag tgtgctaaaa cagcctgatt ttgtttctcg tggaattgat catcaagaaa
     4741 aagcgaaaga aacagataat agaaaaagcg atttaccaga aactggtagt agaaccttga
     4801 acaagctaat tacatggatg ggtgtgttac tcattttgat tgtaggagca agctattttc
     4861 gttctctctt ccatcgagtg aaataaagaa taaaagcaac agatctaaaa ttgaataaaa
     4921 cgatagattt ttgagtgaat atttcattcg agagtgctgt attgcctggc gaagttgtag
     4981 ctttgccaga tctatttttg tttactaaca tatttgagaa acaggaaata acaggaacac
     5041 aaatggaaaa taagagcaag aataattgac aatgcacact gcgcatggta gtatctctga
     5101 aaaatcagta acgaatctat ttgtacgtta ttcatgtagc tgtcggaata agtctaaata
     5161 atagataaaa aggagtatgt actttgtttg aagaatttat tgatatcaat gaaaggcaag
     5221 tatatcaatt tttgaactat tgttatgaaa gagacgagaa attatatgtg gtgaaggata
     5281 tcgctttaga tctaaactac accctggcta agatgaattc ggtgattcaa caagcagaat
     5341 cgttttgcga aaggtatcca gaatataaat tatcttttct ttcagaaaac aagatgataa
     5401 aagtcgagtt ttcttctcag tttttactga gtaaagttta ttctatactt ttagagggaa
     5461 cgattggtta tattttactg gattcacttt acaaaggtac ttatcaatcc ttagaaaatc
     5521 ttagtcaaaa aattatataa gtttgcgtac gattcaaaga aaattaagag aacttaatac
     5581 tattttgaaa aactatcatt tatcttgcag cttgaaaaaa ggtaatccga tagaaggaga
     5641 agaataccgt atccgatact tttttcactt gatgtattgg cagtcattcg acgaaaatca
     5701 ttctgatata ttatcaagta taaaaaataa acgtcaacta ttagaaaaat ttgcgaaaca
     5761 tgcgccatat acgcgtagta tagatagaaa gaaatggatc agcctactaa gaatttcgtt
     5821 gcaacgaatg aaacaaggcc actcgattac gtatgtccca gaagagatga acaaatttgt
     5881 gcatccattg ataacaaaag aagagtttac gcattacata ctagagcctt tttttgaagc
     5941 aaacttctgg tcgatgaatc acatcactcc aacagagcaa gcttaccttt attttatgtt
     6001 tggtgtaatg aacacctatt tggaagaaga tttgttggat actccagata aagtacaaga
     6061 aagttggggc tggattgaat cgtctattgt agaaaatttt gaaaaaaact tcaagatttg
     6121 ttttacaact tcagaaaaaa attatctata tgtcaatttg gcaatgatcc atttgtattc
     6181 aagggttttt gggacaaaaa agaaggtgga tgcttttgga aaatcagcag aagacagaga
     6241 ttttcagcat atatttccag tgatgtatcc aatcatgcaa cagtttttta gtggggtagc
     6301 tcgcagaatt ccagagctta atacttatta tgaaaagaac agacggttga tttttcaata
     6361 ctgtatgtta gtcagggata tttttataaa acacgaacaa cctgtcatat tctatattca
     6421 atcaaagtat ggtaaaacac aggaattatg ggcaaagaaa aggatattat ctatgactga
     6481 acgcccaata aaatatagtg catccgctga tcagttacct gatattgtca ttagcgatta
     6541 tcctatcgac aaaaaggcat atgatcaaac gaatacacag atattttact ggaatggaca
     6601 acctacaaaa aatgattggg cgcgtctgaa agctgaaatc ataaaaatca ggaatgagaa
     6661 aaatagatcc atattggaaa aattggattt cgttaattaa gaaaagcaga ttgtttatgc
     6721 caaaattaag acattgcaaa aagattattt tctttaaaat cgacaagatg accagaaagc
     6781 aagagttcgt tgagctcttg ctttctttta gttttagaaa ggaacatcct gattttatct
     6841 tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga taatcggttt tttcaataaa
     6901 gaaggggatg tacgaatctc aatatatagt tcactcaaac tgagttcatc tattctgtga
     6961 acaagatttt tataactaat agaatgagta gagagcattt cggtcacgcc gttgtttgtc
     7021 aattttagta agtggataaa ttcatttttg gttagtggtt gacgaattaa gttctgttcg
     7081 ataaacggta tatcatggac gataagccaa cgttttgcgc gtcttgaaga cgagcagcca
     7141 gaacctgtat acaatttaat catcatacat tcctccaata gctaagtatt tatgaaataa
     7201 taacatgaag aaatagtcga aaagtgccat ttagaggtgt aaaataagaa aatgttgtca
     7261 cgtataatgg aactagctag ataagaaata ctattaaatc aagaaaaaag aaagaaggca
     7321 caagtatgtc aaacacagcc gacgcaatag tagtgatcga tttacaaaaa ggggtatgtt
     7381 ttgattcttc gccgatacac gaattagaaa cagtgacagc attagtgaat aaaagaatca
     7441 aaacatacga aacgattggg aaaccagtta ttttcgttca acacgaagat gctcaattag
     7501 tgccacaaac aaaaccatgg gagctattgt cggaattatc tattcctgag cacagttatt
     7561 ttgtgagaaa aacccatgcg aattcatttt ataagactaa tttgagtgtt ttattaactg
     7621 aattggactg tcattcgatc gaattatgtg gggcacaagc agagtattgt atagatacga
     7681 ctgtgaagtt tgctcatgga ttaggatacc agcttatgat gtgtagagga gcaacgacaa
     7741 cttatgataa tccttttatg aaagcaaagg aaacaatcgc tttttacgag aaaatttggg
     7801 ataaacgatt tctgacattt ttggagcaat aagttgtttc ttcgtttaaa aaaagcagaa
     7861 tggatactgg tttatccatt ctgctttatt acgtaaaatg agaaaataat cctgttctat
     7921 ttttatggga taaaccattt attttttgta taaaaaatgt ttgcgtttac atataattct
     7981 cctagaaaca taaaaaggag tgaatagctt gagaacaaaa caagtattaa agggagcgtt
     8041 ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg acagtatatg gagagagcat
     8101 ttcttccgaa tcggaatctc gggaggtaga aaccacagaa actactgatg ctggaaatga
     8161 aacgaaatcg agtacttcac aaacagaaac aaatactgaa acaacggttg agacatctgt
     8221 tactagcaca gatcaagaaa cgaccgctac cacagataac caacaaacag aagaagcaac
     8281 agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa gtaagtgaca aagagaatgg
     8341 gacagaaaat gatgatatcg gtaaaggtga aaatttcaat ggtaaagaat atttgaatta
     8401 cttcggtaaa cagatgacag aaatacttaa tggaccaaca gaagacatca agtcaaaatt
     8461 aatagagtta ttagtcactg agcttgctgt taatgattat ggtgtagata aagagattat
     8521 caaacatctg attgctactt atctattgta cgatatcgct tttgcaaaaa acagtgtgtt
     8581 gatgtcgatg cttggaggta cagatgatgg gacaacgcct acagaaaaca caggcttcta
     8641 tccaaataca aaacaacttc atcaaactgt actaaacaaa caagatggca aaatgatgga
     8701 tgtatatgca tattatgtcg atcaaggttc agataaaaca gtcatgatcc atggcggatt
     8761 tcgagggaat tggaataacg ggatcgttac agaagaatac aatgattttt ataaggccgg
     8821 gtataattta ttatttgttg attccagagc gactggtaat agcggaggcg attatgtgac
     8881 ctatggacaa tatgagtcag atgacgtcct ttactggata aatcaagaag ttcgagagcg
     8941 gccatctcaa aagattttac tttatggtgg ttcgatggga gcagctacga tgatgtctgt
     9001 attagcgaaa gatattcctg tcaatgtcaa aggaattatt gaaaattgcg gatttgcatc
     9061 aattgatgaa caactacgtt ttacgtacag ccagacagta gcccccgcac taccggatgc
     9121 cataaaaaat cagttagaca tcattgggga tcaagagcat gaagatttat ttatgggcct
     9181 tttgaaacaa tactattttg atcaggagat gcatttggat acaaaagctg ctctacctac
     9241 gattggaatg tcagattcat tgcctaaatt gattatccat ggaacagcag atgatgtagt
     9301 acctgtgtct aacgcgcaaa aattatatga gttatcagga gggtataaag atcttttatt
     9361 agtagaagga gcagggcatg gaaaggcaca ggaagttgac catgctgcct acacgaaaca
     9421 tgtaacggac tttttgaaag tagtgttcca tgatcagata aacgtaaagt atgtggatga
     9481 aaacaataaa agtctactca aagaccagga cgaaatccag ctttacggag cttatggaga
     9541 aaattatatg acagagcaaa agacattcga aggatacgaa ttagcaaatg ttgaaggacc
     9601 gactgaaggg atttttaatg agacgatacc aactattatt ttctattaca aaaaaatccc
     9661 agtagctcca ccaaaaaagc aagatccgac agagaatgca gacaataagg gaaagaatga
     9721 acctcgagag gatcaatcaa gtgatgatac gcaaccgatt cagatgacag tgaaagtaca
     9781 aaaaaagaca gatgcaaaaa aaagaatgtt gccgaaaact ggagaaaaga aacattcgct
     9841 tctcatttat tcaggtggat ttttatctgt tagcacgatt atcacatgga tttggcgaag
     9901 aaagagaggg taaatgaatg aaatggttca agcgaatagt gattgcagta ccagttatca
     9961 tcgtagttat tttagtcact gtatttttga ttaatcaaat cacaccaaag ccgattagct
    10021 tgcttgttag aaaacaattt gacacaagtg aaaaagaaca agtatatgcc cgaccagatg
    10081 atttccagca aaaaatcgaa caaatcgata taaaaaaaga aatatcctat ccttcaaagt
    10141 acaaaaataa taatatggat atctacacac cgaaagtgga aaagaaaaaa ttaccgattc
    10201 ttttctggat gcatggaggc gcatatgtag gaggggataa aaatgattgt cgtgattatt
    10261 tggaatatct atgttcagac acgcagcaaa tcatcgtaaa tatagattat gaacgatcac
    10321 cggaagcaaa acatcctaca ccagttatcc aattgaacga agccattcaa gcagctaaaa
    10381 aagagataaa agatcaagca gattggtcga atatttcgat tggtggaaat tcggcaggtg
    10441 cacaaattag tggagaatat ttgttagcac ttcaaaatga agaaattaaa aaagctgatc
    10501 atcttgatcc gactttggag aacaagcaga ttacgaagtt tatttctttg tctggtctgt
    10561 tggatccttc tgcacttact caagtaagtg ataaaatctc tagctttctt tatgaaaaat
    10621 gtggatgggc ttactttgat aataaggact ttgaacaatc agagaaggtc aaaagtttag
    10681 ccttggtcag acatgcggat cgttggacgc aaaacacatt cttaacggat ggaaatacaa
    10741 atacgtttac gaaacaaatg gaagaagtaa cttctgtatt tgagaaagcg ggggtgaaag
    10801 tcacaaaagt agattatgcg gagaaaaatg ctgttttgaa ccatgaatat cagtttgatt
    10861 tcagcaataa acaagcgcaa gaaacatatc aaaagttagt aatgtttttc aaggagtaaa
    10921 gtcttatgat gcaaaagagg ttgtaatctg tcacttagct ccaagtcaca aggagcaatt
    10981 tcaataaaca agcttttcat ggtttatgaa tattgtaact tgtgactgag gagttagaga
    11041 tggcaagggg caccttttat tcgaaatttg ggaatatcac acgattattg tcacgatctc
    11101 ttttacgtaa attttataag tcagatttgt tgttttgatc gtttaaaaaa agcaacaaat
    11161 ccgaagcctg cggaggcaag aagggacaaa aagccgagtg gtaagcaaaa gaatgctggt
    11221 tccactaata ttccgttgtt attgatataa ggctgggaac aaagatgaat agcaaataaa
    11281 atcaatgcac tagcgaaaaa aagcaaagaa atatggatag ctttcataag cagcacctcc
    11341 ttttttataa ttgtatgata aatagattaa gaatctataa atgtgacaag tttgttagga
    11401 tgtgaataaa atgatcatct agcgaacttg aaacaaagat ggctaatgat aagcaattaa
    11461 tgattcagca atcagatagg tacaaagttt accatcatac gtctagacca ataatagaaa
    11521 atatgggaat ataatagaaa agatttagga tacccccaaa agaatttccc caaaaattcg
    11581 atgtatccta aactttttta ttttacataa caatcatatc aactgtgaaa taaaattaca
    11641 aatgataacc taaaatgagg ccaaaagcca gtgttatcaa ggaaatactt aattcaccaa
    11701 aatttttttt aattttttag aaaataagaa acaaaaataa taaaaaaaag aaaagataaa
    11761 ttttttacta ctgtatacta actttgttat cgcatctaac aagtaactcg aataatacca
    11821 atacaacctt ctccaaagat gcgatgacat acgttctaac ccaccgacta ttgatgttct
    11881 gtaccaccct tgcagaacca aaaaagccac taccaaatat ttggtagtgg ctttttttgt
    11941 tttatggatt tttcgctttt tctgttatgg tgctattctt tgatttcaga aatagtatgt
    12001 cgaactttag cgatagaacg acgtgattct ttttcgttgt aaaacatcaa aatcacatat
    12061 agagagtcca aaatgcttag ttgagaaata cgggaagcta aagcttcaga tcggaattct
    12121 gtttcttcag aaatagaaat aaaaacgaca tcgccaagtt tagcaaggga agaattagct
    12181 tggctagtaa taacgattgt ttttgctcca gtttttctta cggtttcagc aatatggatt
    12241 gtttcttttg attgccctgt gtgagaaatc aaaacagcac agtctttttc cgtcaataaa
    12301 gaagcttcca tcaattgaat atggtaatcc gtgctgtgag aagttgcgat aggggaacgc
    12361 aaaaatttat gataagcgtc agttgctaca atctctgaac cgccaacacc gaaaaaatag
    12421 acacgtttag attcattgat aatagtagcc gcttttttta gatccttttg ttgtaacaac
    12481 ttttttgtgt catttaaagt tgaaatacta gactcgaaca ccttttgcgc catagtgagc
    12541 tcagtatctg ttttctgaat attttcgtgg atagaaatag cagatagatc attttcttgg
    12601 atcaacatgg ccattttaaa atctttaaat ccattataac caagtttttt tgtaaattga
    12661 aaaaaagtag agtcagcaat gcccagttca cttgctaaat tgctaatagg actatgactt
    12721 actttgcttg gattttctaa aatataatcg gcgatagcct gttcttttgg gctgaagtcg
    12781 ttgtacattg ttttgatttg tagtcttatg gagacgctca tgagaattcc tcattttcat
    12841 aataagatta tcattttttc cgttctcatt aatgttaaag atacaaggag agtaggaaaa
    12901 aatcaaacaa aaaatatttt tgtaaaatct atttacaaaa aaattttttt gtataatgag
    12961 ggtataagaa atggaggcgc tatcatggaa attggtgtaa ttggtttggg aaaaatggga
    13021 ttgaatttag ctttaaactt aaaagagcac catcacaaag tacaaggact cgacatttca
    13081 agtacagctg ttagtcaggc aagagaaaat gacattcctg cttatcaaga gatctctgaa
    13141 tttctagcta cttttgagca gaaaaaaatc atctggttaa tgcttcctgc tggagagata
    13201 accgaaaaca tgttaagaaa actttttcca aaactcggac caggagacat tattattgaa
    13261 ggcggaaact cttattataa agattcaatc cgtcgagcag aagagtttca gaaaaatgaa
    13321 atcggttact tggattgtgg gacttctgga ggaatcgaag gagcacgaca tcaagcctgc
    13381 ttaatgattg gaggagataa agaaactttt ctatccgtgg aacaattatt caaagatgta
    13441 gcaattgaaa gaggatatct gtatgctggt gcatccggaa gcggacactt cttgaaaatg
    13501 atccataatg gaatcgaata tggaatgatg caagcaatcg gtgaaggatt ccaacttgta
    13561 caaaaaagtg agtatgcttt tgacttggaa caagtcgcac gtgtttggaa tcatggttcg
    13621 gtgattcgcg gctggctgat ggaaatcatg gaagatcagt taaacgaaca tcccaattta
    13681 agtaattatc ggggaatcgt ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg
    13741 ctagaaatgg ctgttccagt tccagtaatt gcaactagtt tgttcatgag gaatctttca
    13801 caggaagaag atagtttttc agcaaaagta gtttcagcac taagaaatgg gtttggcgga
    13861 catgaaatag ttgaaaaggg tgagtaaaat gccagattat catatagcaa tcgttaattc
    13921 cagcagtttc ggaagagtct ttccacacca cttggaaaga ttaaagtcag ttggagacgt
    13981 tcatttcttt actgttgatc aagagattcc aggaaaagaa ctagcggagt tacttaaagg
    14041 attcaatatc attattgcaa gtgttacgcc attttttcga cgagattttt ttgaaaacaa
    14101 agatgaactg ctattgattt cccgacatgg tattggttac aacaatattg atttaaaagc
    14161 agcaaaagaa caccagacaa tcgtatctat catacctgct ttagttgaac gaaatgctgt
    14221 agcagaaaat aatataacta atttgttgtc catgcttcga cgcacgacac aagcacaagc
    14281 aagagtcaag gacgatcgat gggaagatcg ggcggagttc gtcggtagga cgctgtttaa
    14341 caaaacagtt ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg
    14401 taacggtttt cgctgtgatg ttctagctta tgatccttac aaatcagatt tatacatcga
    14461 aagctttggt gcaaaaaaag tagaacttca agaattactg gaacaatcag atatcatttg
    14521 tttatgtgcg aatttgacag aagagaacta tcacatgatt tctgataaag aagtcgatca
    14581 gatgaaagaa aatgtttatc tatctaatag tgcaagaggc gctctagttg atgaagaagc
    14641 gatgattaat ggtctgaaaa gcggaaagat cgcaggatat gccacagatg ttttagaaga
    14701 agaaccggga cgtcaagatc atccgtattt agcgtttgaa caagtagtga ttacgccaca
    14761 tatatcggcc tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt
    14821 cgaacagatc agtcagcacc ttcttcctga acgttctgtc cagacaaaga gcaactatat
    14881 aaaaggatga tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc tcaattttat
    14941 ttatatgaag aaggtggtat ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa
    15001 agagtactgg tcacccacgg aacggtatca tgggaaaagg cgctacctaa attagtattt
    15061 ttgaatgacg aaacgattca atttttttat catcgttatt ctggagaatg tagttatgct
    15121 gaagccagac gaatagctac aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg
    15181 gcggaggaaa actgaccgat ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg
    15241 gagtcatccc tactttagct agtaattgtg ctccatggac acctcttgct gtcatgtata
    15301 aggagaatgg acaagcagaa gggaaaacag aacactttta cagacaagct gcttttttta
    15361 taacagatcc ccaattagtg attgattccc ctgttccata tttcattgcc gggttagcag
    15421 atacactagc aaaatggtat gaatctgagc tcatcttaga acaagatttt ttgcaagatg
    15481 aatcattttt gaagctagca caagatactg cgaagatctg caaagaagag attttgaatc
    15541 attctgctaa agcaatccaa gatatggcac aaagaaaact gacatcggag ttcaaacagc
    15601 tttcagagat tatctttgct gttgctggtt tagttggagg attaggagat aaatacgcaa
    15661 gaaatgtggc agcacatgct atgcatgacg ctatcagtaa atatattccg gaaagccatc
    15721 gttttctaca tggagaaaaa gtagcgtatg ggattttcta tcaactggca ctagaaggtc
    15781 gctgggaaaa aatagacgag ttatatcctt tgtatcggga attagaactg ccggtttctc
    15841 tagaacaaat gggattattt ccgaaagata aacagataat agataaaatg gctgaattta
    15901 ttgattcgaa agagaaagtg catttgattc ctatagaggt aacacagaaa tcactgatac
    15961 agtcaatgat ggaactggaa ggttatatca gaaagatctg aaggaggagg acacatggaa
    16021 acattaagtg tagtacaaag tttgttgatt gctttatggg tagccgcggt tatgtcccgc
    16081 tggttaggtg gaggagccac gttgacctta cgtttttctc ctttgatgac tggattagtc
    16141 gtcgggttga tcatggggga agttgcacaa gcgatgattg ttacagccgc tttgcaaatg
    16201 atttatatgg gggttttttc tccaggagga tcaatgccag ctgaaccatc tattgcagca
    16261 gctatcgcag taccagtcgc attattaggg gatctgtcgc cagaagcagc gattgccgtt
    16321 gcggtgccag tcggattgct cggaagttac ttgtatcagt ttcgtttttt catcaatact
    16381 tttttaggta aatatacaga caaagctgta gaagatttga atacaaaagc tatggcccga
    16441 tcaattattc tttatccaac ggttgcctca tttttattat tcgttccgct tgttttcatt
    16501 gctttatatt taggggcgcc attgatcgct gatgtaatca aagctttaga gggaacagtc
    16561 gttatccaca ttcttgaagt agtaggtgga gggttagcgg caatcggtat cgcgacaact
    16621 gtatacgtta tcggtcgaaa agattatctg gtctttttct tccttgctta ttttatgagt
    16681 gttgttttga aatctttaga tattacgatg gttacttatg ccatttttgg gattattatc
    16741 gctttgatct ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac aagcaataac
    16801 aattcagcat cttttgatga cgaagatgac tatgatgacg gattttaatt taggaggaat
    16861 gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag
    16921 atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac
    16981 gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag
    17041 atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa
    17101 gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag
    17161 aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg
    17221 gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata
    17281 tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc
    17341 cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt
    17401 tcactatggg ccgtaatgct acaggcctaa agcaaaacac tggtgttcag acagcgattg
    17461 aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag
    17521 tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg
    17581 atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata
    17641 cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc
    17701 tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt
    17761 tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa
    17821 gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag
    17881 atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga
    17941 aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa
    18001 aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata
    18061 ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt
    18121 taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata
    18181 tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt
    18241 ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga
    18301 acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa
    18361 aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag
    18421 atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag
    18481 atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag
    18541 gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat
    18601 tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc
    18661 ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga
    18721 gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc
    18781 gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg
    18841 tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat
    18901 cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc
    18961 tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt
    19021 tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct
    19081 atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta
    19141 ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa
    19201 tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg
    19261 tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat
    19321 tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat
    19381 ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga
    19441 ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag
    19501 aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca
    19561 aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg
    19621 aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa
    19681 aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt
    19741 ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg
    19801 aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac
    19861 gatgcaatca aggcagggac agatgaacga gattactata ttgataacta tatacgaaca
    19921 atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt
    19981 ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac
    20041 gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag
    20101 tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg
    20161 gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta
    20221 gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat
    20281 ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa
    20341 attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg
    20401 gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt
    20461 gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc
    20521 tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc
    20581 gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga
    20641 tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata
    20701 agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat
    20761 ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca
    20821 aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat
    20881 ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg
    20941 agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa
    21001 aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc
    21061 ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt
    21121 gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt
    21181 tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc
    21241 gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca
    21301 tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata
    21361 tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga
    21421 aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc
    21481 ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt
    21541 atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat
    21601 gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tccacggctc tataatttct
    21661 aggactgata gaaaatcatt ctatcggtcc cttttgagca tgaaaaaaca aaacatacag
    21721 aagctccgtt agaattgaat caccacaaca caatcaaagg agcgttctgt atgcctgatc
    21781 ttcatcatat caaactacta cttggaatca aagataaaaa cattttcatc acaaatgtgg
    21841 aatcgaagag cattaagaat atcaagagtc ttgttgtttc tgcgacgtta agtaaagaaa
    21901 ttcgtcgctg tcctttgtgt aaacagatga atcatgaagg catgatcgtt aagaacggaa
    21961 agaaaaaatc gctcattcaa cttaataaat gtgcgaacca actgacttat cttgctttag
    22021 cgaaacaacg ctaccactgt cgtggctgcc acacctattt tacggcgaat acctacattg
    22081 tcgatagaaa ctgctttatc gctaaacaag tgcgctataa aattttagaa gagctaactg
    22141 aaaaacaagc aatgacgaca attgctaagc attgcggcgt ttcttggtcg accgtttctc
    22201 ggacactcgc ttctctgatc cccatgacaa aagtaaaaag aaattggtta ccgcgttgtt
    22261 tattaatgga tgaatttcgt tcactgaaaa atcaagtcgg tccctactcc ttttcttgta
    22321 tggatggcga tactggaaaa ttactggata ttcttccttc acggaagaaa aaggctctcg
    22381 tttcttattt tatgcaattt gaacgacgcg cacgattgaa ggtaaaggtc atcgtgacgg
    22441 acatgaatgt ttcttatgcc tctctgataa aggaatgttt tccaaaggca aaactcgttg
    22501 tggatcgttt tcacattgtc aaacatctca ttcgtaaatt tgaggatatt cgcgtacgaa
    22561 tcatgaaaag ctttgatcga aacgatccaa cacaagccaa atattatcga caattaaagg
    22621 cgttgtctcg gcttctgatc aaacgacagg acaagcttgt ttatgacaaa tggaccaaat
    22681 ggcgcaattt tggttgggcg tatctcaccg aaagtgaagt ggtggatcgt ctgctttcga
    22741 tctctgacga attacgaatc gcctattttt actatcaaga aattcttcaa gcgtttcacg
    22801 acaaagaggc ggacactttc tttaaacttg taagaacgat gcccaattcc gttccaaaag
    22861 aactgcattc tataaaaaaa gcttttataa actatgaatc cggcattcgt ttagccctcg
    22921 aattgcctta ttcaaatgcc aaaatagaaa atctgcatac ccacatcaaa gcattgaaac
    22981 gagtcgctta tggctttcga agctttcgga aaatgaagac cagaattttc ttactaaata
    23041 atctgattac ctatgaatca aaaaacatct aaagtagaaa aggaattcta ccttagatgt
    23101 tcttatctca ctctatcagt cctatttgac aaagagccgt ttccactcct tatgcagatt
    23161 tttttaattc ttgcactgct tgttcgatcg taacacctgt cgctacatga ttggtatttt
    23221 ctttgtcaaa aacgacaaat agattcaaat ctgtatccaa ggttactcga tattgtatat
    23281 ctttgttata gaatgtcata gccattctcc tttctttaaa tacttgctga aaggcaacga
    23341 acgcgttgat aacgaaattc catttataag tataccacca attgaaaggt tttcata
//
LOCUS       DACEQH010000044        21924 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.044, whole genome shotgun sequence.
ACCESSION   DACEQH010000044 DACEQH010000000
VERSION     DACEQH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.044"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            283..522
                     /locus_tag="IW833_001823"
     CDS             283..522
                     /locus_tag="IW833_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307941.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="IW833_001824"
     CDS             complement(884..1144)
                     /locus_tag="IW833_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307942.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1329..1826)
                     /locus_tag="IW833_001825"
     CDS             complement(1329..1826)
                     /locus_tag="IW833_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ1307943.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1956..2666)
                     /gene="budA"
                     /locus_tag="IW833_001826"
     CDS             complement(1956..2666)
                     /gene="budA"
                     /locus_tag="IW833_001826"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ1307944.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="IW833_001827"
     CDS             complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="IW833_001827"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ1307945.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4548..5210)
                     /locus_tag="IW833_001828"
     CDS             complement(4548..5210)
                     /locus_tag="IW833_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1307946.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5220..6035)
                     /locus_tag="IW833_001829"
     CDS             complement(5220..6035)
                     /locus_tag="IW833_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1307947.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6297..6740)
                     /locus_tag="IW833_001830"
     CDS             complement(6297..6740)
                     /locus_tag="IW833_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ1307948.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6874..7212)
                     /locus_tag="IW833_001831"
     CDS             complement(6874..7212)
                     /locus_tag="IW833_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1307949.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7200..7577)
                     /locus_tag="IW833_001832"
     CDS             complement(7200..7577)
                     /locus_tag="IW833_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1307950.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7801..8994
                     /locus_tag="IW833_001833"
     CDS             7801..8994
                     /locus_tag="IW833_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1307951.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9160..9582)
                     /locus_tag="IW833_001834"
     CDS             complement(9160..9582)
                     /locus_tag="IW833_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1307952.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9977..10171
                     /locus_tag="IW833_001835"
     CDS             9977..10171
                     /locus_tag="IW833_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307953.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            10168..10662
                     /locus_tag="IW833_001836"
     CDS             10168..10662
                     /locus_tag="IW833_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307954.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(10824..11996)
                     /locus_tag="IW833_001837"
     CDS             complement(10824..11996)
                     /locus_tag="IW833_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1307955.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12202..13017)
                     /locus_tag="IW833_001838"
     CDS             complement(12202..13017)
                     /locus_tag="IW833_001838"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1307956.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(13167..13613)
                     /locus_tag="IW833_001839"
     CDS             complement(13167..13613)
                     /locus_tag="IW833_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ1307957.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(13610..14614)
                     /locus_tag="IW833_001840"
     CDS             complement(13610..14614)
                     /locus_tag="IW833_001840"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1307958.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(14654..14983)
                     /locus_tag="IW833_001841"
     CDS             complement(14654..14983)
                     /locus_tag="IW833_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ1307959.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(15109..17436)
                     /locus_tag="IW833_001842"
     CDS             complement(15109..17436)
                     /locus_tag="IW833_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1307960.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(18279..18572)
                     /locus_tag="IW833_001843"
     CDS             complement(18279..18572)
                     /locus_tag="IW833_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ1307961.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(18975..20153)
                     /locus_tag="IW833_001844"
     CDS             complement(18975..20153)
                     /locus_tag="IW833_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1307962.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(20257..20571)
                     /locus_tag="IW833_001845"
     CDS             complement(20257..20571)
                     /locus_tag="IW833_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1307963.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            20678..20893
                     /locus_tag="IW833_001846"
     CDS             20678..20893
                     /locus_tag="IW833_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307964.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(21193..21435)
                     /locus_tag="IW833_001847"
     CDS             complement(21193..21435)
                     /locus_tag="IW833_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1307965.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(21422..21850)
                     /locus_tag="IW833_001848"
     CDS             complement(21422..21850)
                     /locus_tag="IW833_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1307966.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         6453 a         4314 c         3707 g         7450 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga
     1381 tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa
     1441 tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat
     1501 tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat
     1561 gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa
     1621 gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct
     1681 gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct
     1741 aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca
     1801 tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag
     1861 attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca
     1921 ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct
     1981 gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc
     2041 tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt
     2101 acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca
     2161 actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt
     2221 tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc
     2281 ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg
     2341 ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt
     2401 gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca
     2461 cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa
     2521 aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat
     2581 atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg
     2641 tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag
     2701 attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt
     2761 ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg
     2821 attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca
     2881 cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca
     2941 ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca
     3001 gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat
     3061 gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac
     3121 aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca
     3181 tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa
     3241 ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattctgct
     3301 tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct
     3361 gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa
     3421 ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc
     3481 cagtttcttg cttcatattc gattggatca tagccaatag caataactag atcacttcgt
     3541 ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct
     3601 tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc
     3661 tttccaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact
     3721 aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat gtcatctgca
     3781 gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattcccttg gacaggtgca
     3841 tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata
     3901 cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat
     3961 tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga
     4021 tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt
     4081 accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc
     4141 tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc
     4201 acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct
     4261 ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa
     4321 ccttgtttca ttttttcgtc cattgaacat cgtccttttt atgctttatt tttaattatt
     4381 agttagaaat aatcacaaat aattgataca gtttctaaac ctgtttcatt caaaagtata
     4441 gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt
     4501 ttacataaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
     4561 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
     4621 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
     4681 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
     4741 tttggtttcg acgctggatt tctcacctga aaataccagt agttgatgct aaaggaaact
     4801 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
     4861 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
     4921 aatcccttta gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt
     4981 ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaacctgg accaataaaa
     5041 agtaaagaaa aaataaagga aaagacattc cttccatcta tcgacaaaag gaacgcagct
     5101 gctatataca agatattgac acaccaaaaa caaaggctac ttgccaatga ttgatacaac
     5161 caataaaaga attccatata aacatttttg tcaaaacgct gctctttcat aatcacctcc
     5221 tattccttca ttcctgatga agcgatccct tctacaaaat atttttgcat cgctaaaaat
     5281 ataaaagcaa caggaagaac agaaacaact gaggcagcca tgattaatgc gtactctgca
     5341 tcaaatagcc caataaacat tttcaaccct aactggattg ttttattttc cgtagaagac
     5401 aaataaatga atggccccat ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa
     5461 ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc gatagattcc ccactctgat
     5521 aagccatcga tccgcgcact ctcacataaa gaatctggga tactactgta gtattgcttc
     5581 aacaagaaca caccaaaagc attgaacgct tgtaatagca ccaacgacca caacgtatat
     5641 gttaaaccca cttttctcat cataatgaac tgcgggatca tataagactg ccaagggaca
     5701 gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa
     5761 aacccgtaag cagcaaaact agaagtaaac aactgaatca acgtgatcac aacgcttaaa
     5821 aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag accaaataac cagataattc
     5881 tctaaatgcc aagttttagg gatccattga atcggaatcg tgaacacttc attatttgct
     5941 ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg tcaataaagc taatacaatc
     6001 agcagaatca tacccaacca ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt
     6061 tttctttcat ttcgaacacc tccaaagtta taatcttcat ttctttgtaa tgattacaat
     6121 ccagaacaac ggttattata gctgattcat ttccgttctt cttatgtttg taagcgctta
     6181 tttaacaagt tatgtaaatc tctttatttt ttttgattta aaaatgaaaa aatacaaaag
     6241 caagactgtc atacgtctcg cttttgtatt gaaatttctt cgattagaga tacttattac
     6301 tgttcaaaag cttctttgaa acggtgaatc aattcattga ctacaggtga taaaatccga
     6361 ttctttttcc agactagcat gcagttcgtt tcaatcattg ggtcaagcgg aacgaatgtc
     6421 actcgctttg aaagatgact tacagcacct tctatgacta aagcagcacc tacctgattc
     6481 tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc ctgtgatcag caactgctcc
     6541 ttttttatgc ctaaccagtc agcaaacatt cgctgtattt ctggacgctt tgagatcaaa
     6601 agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt tttttgctaa aacagaattt
     6661 tctaaaatga gcaatcccca tttttccttt cttggtaaca aaatcgtttc gtatttctct
     6721 gtatctactg gatctagcaa aatcgctaaa tctaacaggc ttttttctag tcactccgtg
     6781 atatcatcac ttgttccgct gtggatatga aattgaacgc tgggataatc acggatcaac
     6841 ttttctagta atagtgccaa tgtattcgaa ttatcagctt ccacacagcc gatcgtaaat
     6901 cggccgctga ataattgttt ttttctgttc tcgaattctt gagtggtttg attcgttaaa
     6961 tctaaaattt cttctgctcg gcttttttag aaaataacct gcttctgtca agatcatttt
     7021 gcggttttcg cgtacaaaca gttctgttcc tagttcctct tctaactcct tcagttgtcg
     7081 gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct tgagagattg ttccttcttc
     7141 tgcaattgtc caaaaatatt ttagtaagcg taactccata gcgatgctcc ttgatttttt
     7201 tacttgatac attccatttt atttctagat aacacacgat catatttctt gatttttcct
     7261 tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt tcgtcaaaag ttcactttct
     7321 tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc cttcgctaga taggcgaata
     7381 tattcaatca aagactcgat cgataatccg gcatttcgca tacagatcgt aaagtcgacc
     7441 cattttaggt cttcttctgt ataatcacgg attccgcctt ttgtccgatt aactggtgga
     7501 atcaacccga ttcgttcata atagcgcaat gtatcggccg aaatgccctt ttcttcactg
     7561 acttgtttga tattcatctg atcactcttt ctcttaattt cttgccactt tacttatgta
     7621 ttttatttat tctacaactt taagtgtact ccaagtcaat actcatttct attaaaacta
     7681 agctggatta tctatatcgg ttgtttgtgt attatttcaa ataaaaaagt cttgtttttt
     7741 tattcttttt tggatatact tttaacaatt tagggaaatt ttaggaaaag gggtataaaa
     7801 atgacaacgt atcagcgaaa ggtattatta tcgacatcgg cgggaattgc tttagagaat
     7861 atggatatta tgtttttagc tttttcatta tcttcaatga ttacttcttt tcatattagt
     7921 ggtacgcaag cgggtttcat ttcaacgatc actaatttgg ggatgctcat tggaggtatt
     7981 ttttttggca tcatagctga taaatatgga cgtgtgaaag tattttctca aacggtcctt
     8041 ctgttttcga ttgcttcttt attgatgtat tttgcttcaa acatctactt ggtttatctc
     8101 tttcgcttta tcgcaggtgt aggagcggga ggagaatacg gggcgtgtat gtccttgatt
     8161 tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt cggttgctgg tattggtgcg
     8221 caggtcggtg cggctttggc tgcgatttta gctgctgtga tcattccaac gtttggttgg
     8281 aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg tcttgttcat tcgtcgcggc
     8341 ttgactgaac caaaggaatt caaatctgta caatctgccg aaaagaaaac caaacttagc
     8401 agactgtttg aatcaaaaca tcttgcttgg caaacatgtg gccttagtat catggtaact
     8461 gtccaaattg ccggctattt tgggttaatg aattggctgc cttcaattat gcaaaatcga
     8521 ttagggcttt cggtttctgg gtcttcattg tggatggtca gtactatttt aggcatgtct
     8581 ttggggatga tgacttttgg cataatcatg gataaattgg ggcctaaact agccttttct
     8641 ttgtttttaa tttgttcatc cttatctgtc tatctacttg tgttagccca tagccagtgg
     8701 agtttggtag cagccgctgt gatagtcggc tactttatca atggcatgta tggtggatat
     8761 ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg cgacggcgaa taatttcatt
     8821 atgaaccttg gacgagctgt tggtggtttt tcttctatcg tcatcggttt cttgatggat
     8881 cattatagtt taactgccgt aatcttgttt ttgagcggta tttacctagt cagtctgctg
     8941 gtcttgacaa ctcttactgg tgttacagca ttaagggaaa gcttgcagca ttgatagttg
     9001 agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc atctgcggta ataagaccag
     9061 ccaaatcaaa aacatgagac actgtttccg tttggctggt cgtattactt gatgcagggc
     9121 agctttttca actgctaatt tccttctatc tggcggcgac tattctttga cgtccgtcat
     9181 ttcaaagtac tttactaata acttagcata acgaaaaact gtggatacac tcgtatattg
     9241 agcgtctgca taatcttcca aatttttctc aggatgttga tacatgaatt caatcaaatg
     9301 ataattatat gattttctgt agaaatgaag ataaataatg tagattggga agttctcctg
     9361 cttttccata gaaaagcgcc gattattctc attcttcaaa atgaccttat caggatattt
     9421 ttggaacaat tcttctaaat ctgttagata attcaagata gacttacttg ataccttcaa
     9481 ttcgttttgt agttcttcta ttgaacacca acgttctgtt tcaagcagta aatgaatcat
     9541 tttaattttc ttttgagaaa cttttgttaa aaaagtctgc atattttctc ctcctgtatc
     9601 tactttacta tactacatac aagaaaaaaa acggatacaa atatcatatt gtcacaccgt
     9661 caatagtgca taaacctgtt attatttctc tttttttcct aagtattcta ttgatatgaa
     9721 tcatttaatc gtttattata taaatgtcaa taatctaata ataaaataac gtacatattt
     9781 tattattgca tacttaataa cagaactata gcaaaaaatc ccgtgtatca acacgggatt
     9841 agattaagtt tcctcaatca ctattcataa cacaattatt ataacaagta agtaaacaat
     9901 tgaaaaggac atttattgaa aacggttgtt ttttacttaa ggaggaaatt tagcgaaaaa
     9961 attagttgca ttgtttttgg tcgcaggaag tttgttgagt gggtgtggtg ctactacgtc
    10021 tactactcaa agcacaagtt cagatcaggc ttccagcagc agtaccacga tctccgaaag
    10081 tacggcagat tcatcgaaag aacaagaaca aattgaaaag aaaaaagaag agaaacaaaa
    10141 agcggaaaaa gctgcactag aaaaaaagtg acagaagcgg aatctgcttt gtcacaagct
    10201 gaagcaaatc ctacaaaaga aaactatgca actgcttcag cagctatcca agcgctacct
    10261 gaaaacaaac aagagttgac aagccgtctt gctgcaattg attccacaat caaagcaaaa
    10321 gaagcagaag aagaacggaa aaagcaagaa gaaattgcac gacaacaagc agaacaagcg
    10381 caacaagaag caatcgcaca gcaacaagca gaacaggcga ggcaagaagc aatcgcacag
    10441 caacaagcag aacaggcgcg acagcaatca gcagctgaac aggcctctca gcaggcacaa
    10501 gccgcagctc ctgagcaaaa taatgaacaa actgtctatg tcacaccgac cggttcaaaa
    10561 taccatacac ataaatgtgg aaatggtacg tatacccctg ccactttaag cgaagctcaa
    10621 gctcgtggtt taaccccttg ctccaaatgt tatggaaact aattcaaaag ttatactata
    10681 ataataaaat tcttctgaaa cataaaaaaa tagaagatgg cgttccagat agtaaaccaa
    10741 aaataacagt ttacacgtcc atttcgtcag cttctctctt ttttcattta gtctgttccc
    10801 tctgcttttt tggacatctt cattcattct ttctcaatca aatagtacac ctctttttct
    10861 tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa actgtttcga atgtttatgt
    10921 tttacatgga ttttaagcag gagctcctca tgttgcaagc gatagttcct gccttttaaa
    10981 tcagaaaatc tcactagtcc gtattcatca gaagctttca caaatggaac tttttctcta
    11041 gtaatatagt gtttgccgtt gagcgttttt acagaaaaga ccattccttc gattggtcgt
    11101 ccgtttgtgt tctttacata aaaagataaa ggatagtttc gcttagcgat ggccaaagaa
    11161 tctaaataag cgatgagtag ccaaactaag ccgattgatc caagtagtat accgataaac
    11221 caaataaaaa atttccattt tttccattga tcaattcctt ggatcgcttt ttttgcctct
    11281 tttgcttgat aaggaatacg atgccccgtc acaagcagtc gatgtgtatt gaccatataa
    11341 ggagtacagg taagcaaagt cacgagatct ctgccttttt ggatacccaa agaatctact
    11401 tcatcaggta agaccacttg gattttttct acttgatacg ccaatgtctt tccattgatc
    11461 tggatgaaaa actggtctcc ttttttcaac ttaggcaaat ctgtaaataa ttttgcttct
    11521 gggagtccac gatgcccaga aagaacggca tgggtgtttt tcccgccagt cggataagaa
    11581 ctttcttcca ataatgacgt tcctttttgc aaaaatattt ccgttgtctg atcgaatata
    11641 ggtaagctga cattgatttt tgggatttcg atgactccaa gcatatgttc cataaaaaag
    11701 gcattactag gtaaatcttt taatgctttt gcatcaactg cttggttgaa actgcctatc
    11761 cctggtgata cgttttgctc tgccaactgt tggtttttct ttgtcatttt ttcctgctgt
    11821 ttttttattt cctctgaatt cttattcgat gcttgctttt gataatggat gatcatttgc
    11881 tgggcaagaa aatcattcag tgaatcttct acaaaaggat aagaaaagat cccgatccca
    11941 acgactaaaa tgattgccat gaagatgtcc agtattctta ttctgctttt attcatcatg
    12001 tccacctatt ttctaatatg tatatgaaga ggttgtgaaa tcagcgcctt gacctgagta
    12061 ttagcgaagc aaacggcaaa atagttcctc ctattttgcc gtttgttgag cgaataccaa
    12121 gagaccgacc acttgtcaca acctttctcc ccatttgaat cactatcgtt tatttatcgt
    12181 atgatttttg tttccaatag tttaagcttc tgtttcttga tggcgttttt tgaaatagat
    12241 cactgctcct ccaactaaag cagtacctac caagatgaat acgatgatcc ccataccacc
    12301 agttgaaggc aagattcctt taggttggtt atcgatcgtt gtttcatcag aagttgttgg
    12361 atcatcatct actgtaaatt cagtgtacgc agcttcattc ttaggtaatg cgtagccttc
    12421 tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc agcccttcga ttgaaaattt
    12481 cccttctgaa tcagaaatca atttcacttc ttcccagtca gaacctgtga cttcatctga
    12541 ccaagcatat gggtattctc ctgaggcaag gtttgctatt tttttcatta atttaacagt
    12601 tggatctttt gctcttagaa tagaaaattc tgcatcggct aatttttctt gcgtgttcac
    12661 atcttgtttg atgaagtgga aatcaccgta gttttggaca ttttctacag taatcgtgtt
    12721 atccgtagtt gtattttccc gattgactac accgtttgga ttcccaagat tggcttcatc
    12781 tgcgccatgg atgataccgg ttgtttgttg caattttccg ttttttacta taaaagtagt
    12841 ttctttttgt ggattggtta attgataacc atctggtgct tttgtttcaa ctaaagtata
    12901 attgccgtct attaatccat tgacactgat ggatccttca gtatcagttg tgaatgtcaa
    12961 cgctgactca gcaggatcat agagagcgac accagtgttt tcatcaaaac cggtcacgaa
    13021 ttgattctct gaatttttga tctggaattg cgcccctgct aacttatcta cccctgaaaa
    13081 gttcccagct ttctggattt tgatattgct ttctttgatg acatttttag catataggtg
    13141 aatcgtgtct aaaggtttgt cagggtctag ggtaagctta acacgatatt tggcgacgtt
    13201 tcgacaccgg gctttggtgt ttctacaaag acgtatacag catctttccc atcagattta
    13261 cctggaagag tcaggttagc taatccttca ccagaagtcg ttacacttgc gaagggtgta
    13321 gcgtctgctg gaactgctgt gtctttactt ggatagtacg tagcagtgat gtcatatacg
    13381 ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg tcaattccgt tccgttgttt
    13441 atttgatttt ctggtgctga agtgaatttc aatttatgca aattaaccgt aacattttct
    13501 ggtgtaggtg atacggctgc agaaacctct gcagttccta ggccgagaac gaaaggaagc
    13561 agcatgatag cagttcctaa aaacgtccat atttttgttt tgatgttttt cattttattt
    13621 ccctcctatt gtttcctttt tgttgttttt tataaaacca agctgtccct gttgaaagga
    13681 ctagcaataa ccctaaaatt gagaaacctc caagcatctc ttcgcttgtt tttggaaagc
    13741 ttcctccatt tttttcattt gatggaccat ttgtctgcat ctgaactggc gtttttccat
    13801 tattcgtcgg atttgctttt ggttcgttga tgatttccaa tggtatagga tgaagcggat
    13861 cataactgcc tttttcaact tcaaagagaa tggttgattg actcaatata tatcctttag
    13921 gcggtttaat ctccactagt tcgtaacttc caaaagacaa tccatggaca gaaaatgttc
    13981 catctttact ggaagtaaaa atcgttgctc ttgaatcatc tttggctacc caatgattta
    14041 tcccgttttc ttgcgctaag tattggtcat tttttttgac tagaaattga gcatctgcta
    14101 gtttttgttt cgtttgccga tccactttga caaattgttt gcctcctgtt tggatggcaa
    14161 gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac atcgttatca aatgtcgttt
    14221 tttcactgtt ttctttcaat gtcatttggt aacggatcgt taattttttt ccagcaaacg
    14281 aactaagatc ttttccagaa tctgataaga caaaaccatg ttttattttt tgtgtgtgaa
    14341 atgtatgaat cttgtctcca ccaatcaaaa aatcgatcgt ttctggttct agccataact
    14401 gaggatcagc tgtatctttg atcgtatagg ttcctatctc gttgatgttc tcaggaattg
    14461 ttacggtgat gagataaggg actttatccc catctgcaaa atctgtctgc ttattctcta
    14521 ctgtttttgt taatgtgagt gcagcaggta ttttttgttc gctctttgga tacaagtgga
    14581 tggtcgtcaa tttatgatta gatgaatctg tcacaggaag cacaacgaca aatggtgctg
    14641 atttttcttt tacttaatct gaagtcttta tttccgtaaa tcggtaaaca gcgtcacgac
    14701 cttgctcatc tttgtccgat gcactaaaag aagcaatgcc ctcttcctcg tctactgttt
    14761 ttgtcacatt ttctgccagt attttttcac tatcactttt ttgtgctagt tttctttgtg
    14821 cgtcttccac actacttcct tctgaccgca gcttgtaaaa ttcatccgag acatcatata
    14881 tagcgaaagt aacgccgttc aaccctttta ctccttgaaa gttatccggg atatttattc
    14941 cgtcgttttg actgatactt ggcatcttat cgctttcgaa cattcgatta tgcaaagtaa
    15001 atgtaatagg tgattctcta gctgacacag tcggagaacc tataaaaaag accagtagga
    15061 taaaaaagag aacagcaaat atatccacac gtacattttt tgtttttttc atcgctgttc
    15121 acctgctttt ttcttacgat ggataaaata aaagaagcca gaaaaaataa agatcagtcc
    15181 taaaaagaca aagggcagta gacctatccc acctgtcgaa ggaagcaaga gagtcggatt
    15241 gatcgtcaac ttagcggtat ctgttgcgat aatcgtataa ttattgttta cacttccttc
    15301 gcctaaacta atcgttacta tttgtgatcc aatatctgtc cggctgttaa ctgttagggt
    15361 ataatccaat gattcgccta agacaacacc ggtaacgtgg agatttccaa ccgaaatcga
    15421 gtgactttgg gcatcataat taaaggcagc gtcatcgaca gtaatcactg cgttagcttt
    15481 atagatggta taagttcctg atgtgttggc tatatcagta aatgacaact tgggatttgc
    15541 tgctttgact ttgtctattc cgctattcgt tagtttaatt gtatagctgc cagcattaac
    15601 catatttttt gctgattgtg catctgctgt gtaatccata ctgctaagtg ccacttgtac
    15661 tggagtaagc gaactagtga caccctctgc tagcttgaca tggattgacg ggacacctgc
    15721 ttttccatca tatgtcttac tgccactgag ctctaccgca ccgctgattt gcttaatcgt
    15781 atagacatca gatgtctgca cgatagtaaa taaatagttt gttctggcag ctagtttagc
    15841 tagtccgctt tttgtcaatt gccaagacgt ataggttcca atttcagtag gcgcagttac
    15901 ttcaactccc ttttcattca tcgctttgaa atcagttgga ttccatgtga tttgatcaat
    15961 actgagatca ttgtttaaaa gggatgttga ttctgtttta tcattaaagg catatttaac
    16021 gatcggtaca acagaaagac catcatagta cttactgcca tttgcattga ttgatactgt
    16081 tgcatttgtc ttataatata ggatgacttc agaaatctgt tttccatttg cgtcctttac
    16141 gattacattt ccatttgtat ctaaactagc taaaacactc attgctgatt ggttctttgt
    16201 ttctatcgct gaaacagctg taaaggtata tccgttgata gtaggcgtat tagtcgtcag
    16261 gtcttgtgca gcacttccgt aagtaagatt cattatttta gaaggagcaa tcatctgtcc
    16321 aaaatagtct tgatacttaa tagtgattgg tgtttgtttg cctgtatagg tcaccgtatc
    16381 gacgttactt tctagggctg tgaaagaacc gtttgtttgt gcaaaagaat agcccgttac
    16441 tgctgttgga gtgataacca ctttgtcacc cacatggatt ccagataatg tagtcccgta
    16501 cgttccgatt tttgctgctc caccattgac tgtataagaa aaagttcctg tccgattcgc
    16561 attgacctga accgttgctg cttgataggt tggaaaattt gaaacatttc ccttaccatc
    16621 aacgatctgc ccatttttga tcgagtaccc cgtgaatgga atatcatttg ctaactgtat
    16681 accgattgaa aaagtactca tacctcctcc ggcaggaagc cctccccatg ttgagggtgt
    16741 aaaagaataa cttttatccg cattgggtgt aactgaatcg atcgtatttc cagcggtagc
    16801 cacccccata tagatacttt ttgggaatgt gatagtagta ccatttactg tggagccaat
    16861 aaacagatta tggatatctg gacggttatc aataaaagcg attgctttgg ttacgccttg
    16921 taacgcagtt ttatccgcac tcatcgatcc gttaaccgca acaatgactc catctttggc
    16981 atatatcact gtatatccat ctggtatggt atgtccgttg aaattcgcag tggccgcaac
    17041 agtactaaat gtctggcttc tacctgtttc tgaataagct ttctcccctg ctgcttttgc
    17101 tgctgtgatt tctttttctg tcggatcaat cagttgagtc gaggatgtgg ttgaggttgt
    17161 ctcgttgttg ctagactgat tggtgctttc tatggactgt atttcagaat agggtggact
    17221 atttgtctcc tctgtcgttt ggttagcaaa atttgacgtt tctgtataaa cgtttacata
    17281 aatacttgat aagctcccta agaaagtaaa aatcaagatg atcgaaatca taatgggatg
    17341 taagtatttt cttttagata cagctttgag tctagaaaca aaatgttttt gacatatacg
    17401 ttggatttta ttttgagttt tcttaatcat tttcatccct cctctcaata aaaaatcatc
    17461 aaataatcct ttacacgtta ttctaaattt aatttttatt attttattga tgtatagaaa
    17521 gtttggggct ttttttctct ttttattcta aggttatttt agtcttgtcc tttaatacaa
    17581 agattttatt tttaaataaa aaatactctt tttttacgat tattgaaaaa aaaagataaa
    17641 acttgattaa ttgaagtatt gcttaaattt ggaagctatg agggaagaat aaaagagtta
    17701 ggacgggatt atctactatt tttctttgtt ttaaatattt gttatctttg aacataaaag
    17761 agaaatgtgt cgactgcagt gttgtgtccc tcatcacgaa taaacggtgt tcaactagct
    17821 gatcttgtat tcacttaaaa aatggtaaag tacgagttgc tattccccat tttcaatttt
    17881 ctaacgctca ggacaaaata ctgttttcac aacctcattg gaataaaagc atatatattg
    17941 tttcaagaac gaaatcttgt tatcttttta aagggcatct gtccaaaatc taattttagg
    18001 aggtacgttt tggtatttat tcgtacaaat aaaagagaat tatctttatt catgtaacgc
    18061 atccaattga ttttttcatg tacgctcctg gaacgatttt actgacgatt gcttatttaa
    18121 ctgcgaatcg ctcattagct tttatcatag ctatccatat tttgaataac gttcttggct
    18181 ttgtattata gatacagatg acaaaaaaag ttcaagtatg cggtgtagaa ccaatatcgc
    18241 atacttgaac ttttttcaat gagtataaat taaaacttct aattttcaat tcccatcatc
    18301 tgatatgccc gaccgtctga acttcttttg aaaaagccaa atgtgattag ataacgtctt
    18361 tgttcgacat aatcgccact agaaaatacg ttcagcagta tcgaattaac ctctgtttct
    18421 gtataagtca ctccatggtc aaagcgtttc aagacttctt tcaataggat gtatttcttt
    18481 tttgttgaac ttggtacttg ttttactttt ccatcattca tgaatgtgtc taaaactttc
    18541 atcttttctt gctccgtata agtataaacc atcttctttc tccttttgat tctaagaata
    18601 ctccatttat gttagaatat tctggatttt ttattaaagc ggttactctt aagaaagata
    18661 ttatcatagt gctctttttt ttactactac ttataggtag atttattcta atccattatt
    18721 ctcccctacc aataattaaa gaaaaaactg gatcagggtg ttcaaattac atgctaagaa
    18781 aaaagaaccc ttgaatacca agggttcttt ctcagtctga taacaatgat taacgacgga
    18841 tttctttgat acttgtgaaa agtcgttaaa gtattgatat aaaaggattt ttattacctc
    18901 tgactacata ttgactaatt tgttaaaaaa tcgtttttca agaatcctgt aataccagtg
    18961 ctttcaccgt ttttttaact ctctaaagaa ctaaaagcaa tcagaggttt taccttttca
    19021 tttgacgtta agtggcgata aattttcgaa gttgtttggt cagtatggcc taagcgatct
    19081 ttcaagactt cccagctaca gtgttccgct actaagcttg ccattgtatg acgagctaaa
    19141 tgagtatgaa atggcttatt aattcctgag cgattcacgc accgtttgac gtgatcattt
    19201 acatactctc gacgataagg aacaccacgt ttactagaat caagatacac aaacaaatat
    19261 gtttcttcta taataaacct gtaatgttgc ctcatctcac ccaaagcctt aatcaactgt
    19321 tgtagctttt ccataactag aggagttact agaactcgtc tagcagaact aactgtctta
    19381 gtagtttcaa ttcgaaaatc cttactctta cctttaactg ccgtttgctt atagaacaga
    19441 atttcatttc ttttgaaatc tatatctcca ggctgtagag caagcgcctc gctgactcgg
    19501 cagccagtgt aaaatagaac atggaaaagt acagacatcg gtaattcctc atgaacaatg
    19561 ccataattca aaaaagttct caattcatcg acagtaagaa acttgtcctc aatattattt
    19621 atggcttctt ttaaatcagt taccgttaac tgatattttg gcattctcac agacttcatt
    19681 ggattttcac ttatcaaatt ctttctttga caatatttaa aaatcatatt tagaacttga
    19741 ataagtgatt gtaaactatt tttggcatag ccaacatttg ttccattaac gccacattcc
    19801 tttaaggtaa aaagcaattc ttgaaggcac tctggtgtca aatcagacaa ctttttatca
    19861 ccaatccatc gaacaataat ttttaattgt tcctctcgct tatcataggt tatttccttt
    19921 tgttcattta acgaacggta atattggaac cattgctcac ctacaacact aaacttgtca
    19981 ttgcttgcaa caattttttt cttaagcctt tgttgtttct ccatttccag ataagcgagt
    20041 tgcgcctcac gttttgaatt aaagccacgt cttgttgttc gatatgcttg aaagtaccat
    20101 gtgatcctac cagtagaact gatttgcttc ttgaatcgat ctgttttttc catttattga
    20161 ttcctcctat tttgaaggaa cccaacaagc agaaaccctt tggaaatctt tctatctatg
    20221 tcatcattgt accgcatctt agaaagaaaa tgtaacttat aaagatcgaa gtagtcgcaa
    20281 tttttctcga tagtcattcg aatcactctt tctcatccat tctaaaacat catcacgaaa
    20341 atagtgtcct cgaatcccgt accaacaggg aggaaactca ccaagtttta ctaaacggtt
    20401 cagtgtacta tcacttatct gaaatagtcg ctttaaatca gtatttgtta ataccagagg
    20461 taaatgactg actgcaagca atttttccgt gtaatcttcc agtttttttt gtaaaattga
    20521 atagaactct atttccagag aattaacatt tgttttaact tccaactcca tcacccttct
    20581 ctctcaaaat caaaacgacc accacggtac cccttaaaat tgtaaaaaaa cactccagta
    20641 ctccatcaaa acggactaga aaagaggaat ttttaatatg aaacaaggaa ttgcagacat
    20701 aaaaataatt aaagaaattc ttgaaaaatc tacggctaat gcaatagcat tcggaacagg
    20761 aatcaacttg agtacagtaa aaaaacttaa aagtggagag agagctgaag aaaaattaaa
    20821 tcttgccgat gctattaaaa tcacagaatt tggcatgaaa aatatgccaa ccaaaatcga
    20881 aatatggaaa tagtagtttt tctgatctat cagctgtttt gccaaagcac ataggaatct
    20941 cacctccccc cagtaatggc gggccgattt ggttacggaa gtatcattat cgtagtcgcg
    21001 attctcatat taaaatttgt cgcttaaaag ctaaccttgt catggcgtaa ctagagaatc
    21061 aaaatctaca tgtactgata gatttgctat tcatttgtca aagagcgcta ggctattttc
    21121 agcctatgga aatcctttat tttgacccga taaaaaactt ccatagaatg aaaaggaaag
    21181 ggaaaaaata ctttaacgag ttgtatcaaa ttcaagtatc ttctcaatca attttgtttc
    21241 cagttgtcta cgcatatatt catctacata cataaccatt cctttttctt cttcataaac
    21301 tggtttcatg gctaaccgtg aaatataatt ttgatatttt gacagaacga tggacatggc
    21361 aattgaatca ccagaagctg cggctttgat tgtcgttagt ggtatgaatg gtatatatcg
    21421 tttattttgc ttcatatttg acacccaaat actttcctag ctcttccaaa gccatttgtc
    21481 gttggtacca aacactgccg agacttttcc caataatatc gccaatttca cgatcattaa
    21541 gaccgatgaa gaaggacata aaaatgattt cttgtctttg actaggtaat gctgcaatcg
    21601 catttgccaa ctcctcattg gaaataaaga ttttcatacc aaggacaata aaagtaccac
    21661 tactattcaa atttggttcc aaataataga actctgtacc taaccctttt tgttccaata
    21721 tttccattga tatttgccgt tggcgaaatc tagcgtactt cttttgaata tttaacgcct
    21781 cattgcagac tactttctta caaaaactgt caaacgtatg ttgaatgtgg gtttcctctg
    21841 agtcgcccat aatattctcc ccctcaaaaa attcattcaa ctcgttttac ttctatccct
    21901 ttccattatt actacaatct agga
//
LOCUS       DACEQH010000045        21156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.045, whole genome shotgun sequence.
ACCESSION   DACEQH010000045 DACEQH010000000
VERSION     DACEQH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.045"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..657
                     /locus_tag="IW833_001849"
     CDS             <1..657
                     /locus_tag="IW833_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1307967.1"
                     /translation="FKIVLKTQSPLEVKKYLKQFRPKQGIYFLDIDLNHEVNGIELAE
                     VIRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNEIVELLLLAQER
                     IDATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDGQYEFYGRISEL
                     EKKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQKIKEKLKESTV
                     "
     gene            complement(1360..1575)
                     /locus_tag="IW833_001850"
     CDS             complement(1360..1575)
                     /locus_tag="IW833_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ1307968.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1594..1830)
                     /locus_tag="IW833_001851"
     CDS             complement(1594..1830)
                     /locus_tag="IW833_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ1307969.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2088..4241
                     /locus_tag="IW833_001852"
     CDS             2088..4241
                     /locus_tag="IW833_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAQ1307970.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4210..5625
                     /locus_tag="IW833_001853"
     CDS             4210..5625
                     /locus_tag="IW833_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAQ1307971.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            5658..6692
                     /gene="sppA"
                     /locus_tag="IW833_001854"
     CDS             5658..6692
                     /gene="sppA"
                     /locus_tag="IW833_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ1307972.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            6694..7215
                     /locus_tag="IW833_001855"
     CDS             6694..7215
                     /locus_tag="IW833_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ1307973.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7212..7598
                     /locus_tag="IW833_001856"
     CDS             7212..7598
                     /locus_tag="IW833_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ1307974.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(8094..8166)
                     /locus_tag="IW833_001857"
     tRNA            complement(8094..8166)
                     /locus_tag="IW833_001857"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8131..8133),aa:Lys,seq:ctt)
     gene            8547..9116
                     /locus_tag="IW833_001858"
     CDS             8547..9116
                     /locus_tag="IW833_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ1307975.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            9103..9963
                     /locus_tag="IW833_001859"
     CDS             9103..9963
                     /locus_tag="IW833_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1307976.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            10153..10857
                     /locus_tag="IW833_001860"
     CDS             10153..10857
                     /locus_tag="IW833_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1307977.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            10862..12697
                     /gene="walK"
                     /locus_tag="IW833_001861"
     CDS             10862..12697
                     /gene="walK"
                     /locus_tag="IW833_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ1307978.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            12694..14010
                     /locus_tag="IW833_001862"
     CDS             12694..14010
                     /locus_tag="IW833_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307979.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            14011..14880
                     /locus_tag="IW833_001863"
     CDS             14011..14880
                     /locus_tag="IW833_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307980.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESINAFSNT
                     IVTNNTVPKVEDQ"
     gene            14935..15744
                     /locus_tag="IW833_001864"
     CDS             14935..15744
                     /locus_tag="IW833_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1307981.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(15847..16476)
                     /locus_tag="IW833_001865"
     CDS             complement(15847..16476)
                     /locus_tag="IW833_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAQ1307982.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            16646..17938
                     /locus_tag="IW833_001866"
     CDS             16646..17938
                     /locus_tag="IW833_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1307983.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(18014..18676)
                     /locus_tag="IW833_001867"
     CDS             complement(18014..18676)
                     /locus_tag="IW833_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1307984.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            18901..19185
                     /locus_tag="IW833_001868"
     CDS             18901..19185
                     /locus_tag="IW833_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ1307985.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            19236..20861
                     /gene="groL"
                     /locus_tag="IW833_001869"
     CDS             19236..20861
                     /gene="groL"
                     /locus_tag="IW833_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ1307986.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         7206 a         3427 c         4265 g         6258 t
ORIGIN      
        1 tttaagatag tattgaaaac acaaagtccg ttagaagtta aaaaatacct caaacaattc
       61 cgaccaaagc aaggaatata tttcttagat attgatttaa atcatgaagt taacggtata
      121 gaattagcag aagtaatcag aaaatatgat gttcaagcaa aaatcatttt tacaactact
      181 catgatgaga tgttacccgt aacaataaaa agaagagttg aaacgttagg atttgtaaca
      241 aaagatcaaa cactagatga gtatcgaaac gagattgttg agttattgtt attagcgcaa
      301 gaaaggatag atgcaacaaa acaggttacg aatcaagcat ttgttttttc cataggttct
      361 caaacattta cttttgatat aaatgacatt tattttgtag aagcttcaaa attaccacat
      421 agattatcat tgtgtactaa agatgggcag tacgaatttt atggaagaat tagtgagctt
      481 gaaaagaaat atcctatgtt aacaagaatt agtcgagcgt gtttagtaaa tatctttaac
      541 gttaaagaaa tagattttaa aaaacgttca ctatattttg attcagagtt ggccagaaac
      601 tttactttag ggaaagcaca aaaaattaag gagaagttga aagaaagcac tgtttagtgg
      661 ttttttcttt agggagattg catttttcta ataaaataga ttccttcact agtgcaaatt
      721 tttctgaatc ctattaaagt tgtaccaatt tttatagctg ttattagtta cgtgcataac
      781 agcaatatta gttactggag gaatatttct atggtctgga accaaaaaag ggccaaatga
      841 atagttcttt tcttgttttt attaagtacc ataaagggga attaaaatta aatgtatggt
      901 ggaagatgcg aaagatttgg caaattctac taatttttag aaagtgtttt tcatttttta
      961 gttaaaaaat ttgtcctgtc aagtgcaatc ctatgattgt acttgatagg atttgagata
     1021 attactttcc tgtttttata aatttaactt gcaggaacag gctttaatgc acatccgaca
     1081 agtacattac caatacctcc tgtaacggcc gaaataggtc ctcctgcagc accaactaca
     1141 gcgctgcaag tccttttgta aaaatatctc taccattaca tttccaaaga atttttatct
     1201 gactttaata aaagataaaa atttaatata attataaaaa ctagcacaag aagctttagt
     1261 aaaaagttct gtccattcca aacttttgaa aagataaaga acatacctat gttcgtaatc
     1321 gacacaaacc aatcttgagg tttgatattt tttaattgtt tacgcaccgg tccacatgct
     1381 gaatccagca cctaataaag ctccacctac accacaagca gctattgcat aaggtcctcc
     1441 ggctgaacaa agttttactc ctgctgttgc cccagaaaaa gtagtgccta aaactcccac
     1501 ggcactccca ccttgaatat ttttcaactc ttctgaagat aatataaatg ttgtttttgt
     1561 gtttttaata tgcatactac ttattctcct tttttatttt tttcgagcat cccagccagc
     1621 tgcagcacct ccagcgacag ctccatataa ccctccaaca acaccgcaag ctggtccgca
     1681 aattgctgtt cctaaacctg caccacctac tgcccctgtg gtaagtccac ctacaacttg
     1741 ccccaatttt ccccaagcat tattgcctcc accaacagtt tgtgcatttc cacaatactt
     1801 agttccttta cattttgcat gtttaaacac gcttctttaa ataatatttt ttacggtgct
     1861 actgtaacat acgtatttga ttaacagtaa aaatcttcct gaaacttatc aagtttattc
     1921 ttgaatagta aaatttccaa aataaatttg attcaggaat gaaaaagtta gtgttcaaga
     1981 actgttttca aagtttttgt ggaattatga gacaatattt tcactgtatt aaagctatat
     2041 tgagaatttt tctataaaaa ttttttagaa ggagttgtgt tagataagtg ataaaatcat
     2101 ttccttttgt gcagcaacaa gatgaaaaag attgtggtgt tgcttgcctt tcgatgattt
     2161 taaaatattt taaaactgat atcccgattc ataaattacg ggacctatct gggactgatt
     2221 tagagggtac ttcagcattt ggattgaaaa agacatttga aaaattgtct tttgattgta
     2281 tggctattca agcagatagt gagatctgga aagaaagaga tcttatctta ccattaattg
     2341 cacatatatt ggttgataac agctatatgc actacgttgt aatttataaa acgaagggaa
     2401 acgtactatg gattgcagat cccgctaatg gaaaaaatag aaaaacgatt gaagcgtttc
     2461 aaaaagaatg gacaggtatt cttttattac ctatgcctag agaaacttat attcctatta
     2521 aagaaaaagt agctggatta agttcttttt tgcctataat ttggaaacaa aaaggattag
     2581 tattccacat cgttttagca tcgattttca ttacgttttt tggcatagga agctcatatt
     2641 attttcaagg tattcttgat tttttcatcc ctaatcaagc acgttcaact ttaaatataa
     2701 tttccattgg cttgatcgct gtctatttat ttcgggtatt gtttgaatat agtcgtagtt
     2761 atcttcttat tattttagga cagcgaatga gtatggcaat tatgcttcag tatttcaaac
     2821 atgtgttaac tttaccaatg aatttttttg ctacgagaaa atcaggggaa attatttctc
     2881 gttttcttga tgccagtaag attattgatg ctttagcaag tgctacgtta tcaatatttt
     2941 tagatattgg tatggtgttg cttgttgggg ttacgttagc tattcagaat agtacgttgt
     3001 ttttaatcac tctatgctct ttgccttttt atgtagttgc aatattcgct ttcgtgaaaa
     3061 gctatgaaaa atcgaacagg gaagaaatgg cagccggtgc agctgtaaat tctagtatca
     3121 tagaaagtct taaaggaata gaaacaatta aagcatatag cggtgaagag aaagtatata
     3181 accgtataga ccatgaattt gttaaattaa tgaaaaaatc atttcgtacg gttacgcttg
     3241 ataatattca acaaggaatt aaacatttga ttcaattaat tagcagtgca ttaattttat
     3301 ggctaggttc ttattttgtt atggataata gaattagtct aggtcaactt atcacataca
     3361 atgctttact agtatttttg acagatcctt tacaaaacat tattaattta caagtgaaaa
     3421 tgcaaacggc tcaagttgca aataatcgtt taaatgaaat tttcgcgatt gattcagaaa
     3481 aatcgaacca gtcttttgaa tatatgcttt ctgaacaatt atttcaacaa gatatcactg
     3541 taaaaaacgt ttcattctcg tatagcatga aggccccaac gttgaacgaa atttcatgta
     3601 caattccttc gcacagtaaa acggctatag ttggagttag tggttctgga aaatcaactc
     3661 ttgcaaagtt atttgttcgt ttttatgaag catcagaagg atcgatccat tatggaaaca
     3721 ttaatcactt agacattcct tttcagatat tacgcgatcg agtgacatat ttgcctcaag
     3781 agtcattttt ctttagtgga acaatcattg agaatcttat attcggttta gatagcccac
     3841 caagtttcga acaaataata gaagtttgtg agatagtcca actaaatgaa tttattaatc
     3901 aacagccatt acgttacgag acattgttgg aagaaggtgg aaataatctt tcaggagggc
     3961 aacgacaacg attagcctta gcacgggctt tattaaaaaa tccggacata ttaattttag
     4021 atgaagcaac tagtggatta gataccttgt tagaacatgc aattatgaat tatctcctca
     4081 atttaaaaga aaaaacgatt atttttattg cgcatcattt accaatagca aaagcatgtg
     4141 atcaaatttt agttctccat gaagggcatt tggtagaaaa aggaacgcac gatgaacttc
     4201 gttttgccga tggtatctat caaaaattat gggaaatcta ggagagggaa acaatttatg
     4261 cagaataata attggttcga acattctagt atctatacta aacaacatca tagcttctac
     4321 cgttgggtgt tataccctgt aattcttttt ttatgtatta ttggattgtt tttaaccttt
     4381 gctaaaaaag aggttgttat tcggacaacg gctcaattga accccgaaaa aatagagaag
     4441 ttacaagtgc cattagaagc taaaataatg gaaaacttcc taaaagaaaa tcaatttgtt
     4501 cataaggacg atgtgttggt tagattggat tgttctttaa tagagaatga aaaggctcag
     4561 atagaacaag aaaaccaacg tattactcaa caaataaaga tggctcagct atttattgaa
     4621 agtataagta aaggaaaaaa tttgttttca acggatgaca gttttggcta cagtaatcaa
     4681 ttaaagagca tgttgtcaga aaaagaatca ctccgctacg ctttgaagca aagtgaatta
     4741 aatgatcaaa agcaattaga agtatacgaa aagacaaaaa gacaactaga aaaacaaatt
     4801 gagagttcag atagtaaatt acaagaatgg caacaagtac aggtagcttg gagtaataat
     4861 caatcattaa aagatttttc aaaagaaatg atggcaaact atgagaattg gcaagaacaa
     4921 ctaaataatg tttctgatga tcaaaaaaat caagtgaaac tgacaatttc agcaagcata
     4981 aatgaacaaa ttgagcaact aaaaaaagaa gtagaacagt atcagtcaga aaaagctaaa
     5041 ttagttaaac caactacttc tgagaatgac agaattagtc aaacggaaaa aggaaagcaa
     5101 gagctagaac aagcaataac aactgtaaaa caaacagtta cagagctaaa tgaaaaacaa
     5161 gagaagaacc agtcagttat caaaagtttg gacgatcaac tttcaaaagg aatactaaaa
     5221 gctcctgtat ctggaacggt tcatttaaat gaagaaacaa agggacaagc agaactagcc
     5281 aaaggaacag tgcttgcaga aatttatccg atccacgaaa aatcaaaaat gaaatttaca
     5341 gcacttttac ctgcgaatga aagtacctat attaaagaag ggatgaaggt tcattttaaa
     5401 ttggatcaaa aaggtggggc tccgattacg atcgacggaa ctttagatga aatttcagaa
     5461 aattctactg ccactgagaa aggtgttttt tatgcagtga aaggaacgtt aaaaacaact
     5521 caatcgtttc cgtttcgtta tggattaact ggagagttgt ctttaattgt aggagaaaaa
     5581 acatattggc aacaaattgt cgatgtgttt ctaaataaaa aataattatt tttactaaga
     5641 atataggagg agtttatatg aataaaagaa gatggattgc tgtgggagta gcgtgcggat
     5701 tattgttact atccattatc gtcgcgctaa ttccaggaaa agataaagag gaagcatcaa
     5761 atacaaccct aacaggaata aataagattt tttacggatc taacgaaatc acagaagaaa
     5821 cgttagaaga aggcgcatca aataaaaaga ttgtcaaact atccgtcaat ggagttattg
     5881 cagatacagg tgaaagcaat ctttttagta gggaacaata taaccatcag aattttttaa
     5941 cacagctaaa gaaaattcaa gaagacaaag cggttaaggg agtgttatta gaagtaaatt
     6001 cacctggggg aggaatatat gaaagtgctg agattgctaa agaaatggca aaaattaaaa
     6061 agctagatat tcctatctat actgcattta aaaatacagc ggcaagtgga gggtactaca
     6121 tttctgctgg ttcagataaa atatttgcta cggaagaaac aacaactgga tcaattggtg
     6181 tcattatttc tggtttaaat tattctggct tattagaaaa acttggcgta actgatgcga
     6241 cgtataaaag tggtgcatta aaagatatga tgtcacctca acacaaacct tcagaggaag
     6301 agaataaagt tatacaggaa tttgtgatga gtgcttatga tcgttttgtc aatgttgtag
     6361 caaaaggaag aaatatggat actaatgcag tgaaagaact tgcggatgga agaatttatg
     6421 atggtaacca agcagtagaa aatgggttgg tggatcaaat tggttactct gaagatgcat
     6481 tagattccct aaaaaaagag aagaaattaa ctgatgcgac ggttatagaa tataaaaatg
     6541 acacaactgg ctttgcttct tcttggttag gtaataagat tgctgaatgg caagggctaa
     6601 aggcaactac atcagatcgt gtaatttcag tttttgaaaa acttggattt tctgatactc
     6661 caaaaccaat gtattactat ggagggctat aaaatgaata agaagagtgg cattgaaact
     6721 ttgcctaatt atgtatttgc gggattttgg ttacgatttt ttgcttttct tattgattta
     6781 atttgtatta gcatcatcac ccatgctact ataggtctct tttacaaaat catgggactg
     6841 gaatctgaca aatccattat tactttttat ggtatagcgt cattagctat ttatttaacc
     6901 tattttgtac ttttgactaa gctgaattca ggacaaacaa ttgggaaaat gatttttggg
     6961 ataagagtag tgagctttga ggaaaaagag ctaagttggc aaactatctt tattcgtgag
     7021 tgtatatgta ggtttatatt aaaaaatttt gtatttcaaa ttgggtattt ggtggctgct
     7081 ttcacgccca agaaacaaca tattggggat tatttttcta acacgtcagt ggttgttatt
     7141 cgttcattag aagctattaa ctatggtcaa aaaatttcta attctaaaac acttaatctg
     7201 gagagaatta aatgaataag agaaaaacag agtttatttt gattttaatt agtggaataa
     7261 cagggatttt atgtagtctt ttagcattga tgggtggtat gtttctatct tctattgatt
     7321 caactacttt aaatactaca gaactaacca gtaaggatat agatatgtta gtcaatctaa
     7381 gcgggaatgt tgttttattt ggaggaatta ccctagtttt ttcgataatg attttcataa
     7441 taggtttttt agtaagaaaa aatcaaaaaa cagtggtcta tggtattagt ttactcattt
     7501 tatctattat tccatttttt cttttaacat tattgtgggt aattcctgga gttttaggta
     7561 ttattgcggg attgatgctt ctaataagaa aatcttaatt aggcaatgtg tagacaacgt
     7621 gaaacgatta atcaattaaa agctaaatac gttttgagaa atgataactt gtagtcgatg
     7681 gaagacatgt agataaaaaa actacgtgtc ttttatttat tctaattttt tcgaaaaaaa
     7741 gacactctct gattgttgta ttaatggaaa aggggaaagc agcaagtaaa tttagttttt
     7801 ctattaaata ttcttcactt tttaaccttt tatagtactt agatttaatc ctatatgttt
     7861 agagagttca ttggcagcat gattttgcaa agcttctcga attattgtta tattagaaac
     7921 tttactgttc ataattatgc ttctttttta actcaaagtc caaagggcta cagtggactt
     7981 tgagttaaaa ataaaaagcc ctagttattt tgaaaaataa aaaaagaaca gcgtaaatgt
     8041 tttcgaaatg ctttcgcacc aacatttacc tgttccgtgg gtttggcacc caatgggccg
     8101 tgaggggctc gaacccgcga cccgctgatt aagagtcagc tgctctacca actgagctaa
     8161 cggcccgtac aaaaagtact taataatagt agcactaaga attttaaaat gcaagactac
     8221 atttaaagaa aatcagaaaa atctttactt ccttatagga aatcattcat agattattca
     8281 catttgctcg ctattatata agcataccaa caaacaaagt tcttgcaaga gaacaaaccc
     8341 taacactttt ttcatttttt actcctttcc cgccgtctcc ccggcgggtc tcctgttctt
     8401 aacgataaaa gacaagaagg cttagccttc ttgttttttg ttttcatgga atcccgctaa
     8461 tcaaatcacc tagttaaagc aactagattg aatgtttatc ataagtctga tatcatgttt
     8521 atgaaatgaa aaaaggagcg gttatgatgg aaaaaatgcg tcagcaatta atggagtggg
     8581 ggaaggaact agaaacattc cgtctgcctc actgggaaga actccctgac ttagaacttt
     8641 atatggacca agtcagaacg ttagttgatc gttatctttc acctgtgata caaggagaaa
     8701 agcatccatt gcttacctct tctatggtaa acaattacgt taaactaggt ttgataccag
     8761 caccagttaa aaaaagatat aacaaagaac acgtagcatt tttacttgca atcactacgt
     8821 tgaaacaagt attgactatt cctgaaatca aagaagggat attgttccaa ggaaaaacag
     8881 taggaattcg tgaagcctat aatctatttt gcgatgaaca agaagcagct gtgtggatgg
     8941 tcagccaatt agcacaaggt aaatctcatc cgcaaaaatt cgaccaggca acccctgtag
     9001 aatatattgc tgtaagagcg gcaactttat cgtttgcaat gaaacttctt gcggaaaaaa
     9061 cgattgttct agaaaccgaa tatttaaaag aggagaaaac aaatgaaaaa caataaaatc
     9121 gcaattttag tagattcagg tacggatgtt ccagaagaat tgataaaaaa atacgagatg
     9181 tatatgatcc cattgaaaat tatttataaa gatcaggtat atacagataa aattgacatt
     9241 acccctgaag agatctatca acgtttgcca gaggaaatcc ctggtacatc tttgccggat
     9301 ggtgagtcga tcacagaaat ctttgagcaa atcaaaaaag acggttatga aaaggtactt
     9361 gcggtaacaa tttccagtgg attgagcggg acatataatg tggttcgttt aattgcacaa
     9421 cagcaagaag aattggaagc atatgtacta gatacgaaaa atattggtat tggtgcaggt
     9481 ttttctgcga tccaagcagc aaaatggctg gaagaaggag tagaatggga tcaactgatc
     9541 agtaatctga atgagttagt cgaaagaaca aaagttttct ttaatgttgc gacattggaa
     9601 tatcttcaaa aaggtggacg gatcggttta gttgcttcta ttgtaggaac cgctttaaag
     9661 cttaacccaa tcatctcatg taatgaagaa gggatttatt acacagtagg aaaagctagg
     9721 ggacgtaaaa aaagtttgga taaaacggtt gcttacgtca aagaaaaaat cggtaatgca
     9781 aaggtcttca acttagctgt tgcccatggg gatgcgaaag aagaagcaat tgaaatggaa
     9841 gcgagactta aaagagagtt cccacaagca aaccaaatct actttggcca gatttctccg
     9901 gcattagtag tccacactgg tccgggatta ttgggtgtcg gtgtacaagt tttagaagga
     9961 taaatcaaaa aaaggaaaca ctgaggtgaa aatcagtgtt ttcctttttt attgaagtta
    10021 gaacaaaaaa taataaagta ttttgcactt tcattttaat tttctctttt gttttatagg
    10081 caaagatgaa acaaaagcag aaatacggta taatgagaag gtatgacaat aatatataag
    10141 aggtggctct ttatgaaaaa agttttagtt gtcgatgatg agaagccgat ttcggatatc
    10201 gtcaaattca atttagccaa agaaggctat gacgtttata ctgcatatga tggggaagaa
    10261 gccttggaaa aagttgctga ggtagaacct gatttgattt tacttgattt gatgctacct
    10321 aaaatggacg gcttagaagt tgctcgtgag gtacgcaaaa cttatgatat gccgatcatt
    10381 atggtaacag ccaaagattc tgaaattgat aaagtcctag gcttagagtt aggcgccgat
    10441 gattacgtga ccaagccatt ttccaaccga gaattagttg cacgtgtcaa agcaaatctt
    10501 cgtcgcggtg ccacggctgc gaaggaacca gaagaggcag cacctgcgga attgacgatt
    10561 ggtgatttga cgatccatcc agaagcttac atggtgacga aacgaggaga aacaatcgag
    10621 ttaacacatc gtgaattcga actactcttt tacttggcga agcaccttgg acaagtgatg
    10681 acaagagaac atttattaca aacggtttgg ggttatgatt attttggaga tgtacgaaca
    10741 gtggatgtaa ctgtacgtcg tttgcgtgaa aagatcgaag ataatccaag ccatccaaat
    10801 tatttagtga cacgtcgtgg tgtcggttac tatttgcgta acccagaaca ggagtaattg
    10861 gatgaaaaga aaagtccgct tttttcgatc agtcaatttt aagatcgcta ttacctttat
    10921 tctgatttta ttgatctcga tcgaaattat tggtgcctac tttatacgtg gtttagaacg
    10981 ttccacgatc aatacattta ttaaagatat gaatcaaacg gtggaatcat tagcaacaac
    11041 aatcagcccg gaattgaatc gaaaagataa tgcagatgat gaagaggtaa atgcgaatat
    11101 caaaagattt attgaaaaca gtgcgactag cgatatcatc gaaatacggg tcgttgacga
    11161 aaaagggatc atccgaggaa caaccgatgt caatgaacaa agcgctgtgg gaaagaaaaa
    11221 tgattatgtt gatatcaatg atttcacgac taaacgttat gttgctttag ataacgacga
    11281 taaacgggtg aatatcaatg ttcaaccgat cctgtccccg accggcgatg ccgttatcgg
    11341 ggctctttac gtcaaaagta atatcgaaca aaaatattcg gaaatcaata acacagcagt
    11401 cattttcttt actgcctcac taattgcggc atttatttca atgatcgtat ctgttttagt
    11461 tgctcgttcg attacacagc cgatcggtga aatgagagaa caagccattc gaatcgctag
    11521 aggagactac agtcggaaag tcaaagtgca tggacaagat gaacttggac agctggctga
    11581 taccttcaat cagttagctg aacgtattga agaagctcaa gatacaatgg aagctgaacg
    11641 caatcgtttg gatagcgtgc tatcccatat gacagacggc gtcattgcga cagatcgcag
    11701 gggtaaagtc attacgatca atgatatggc tgtttccttg ctagatatca aaaacgagga
    11761 agcaatcgga caatcgattt tgacactttt agatatcgaa gaagaatata cgttgcggaa
    11821 attattagaa gatccaaacg aaatggtact tgaccgttca accagtacat tggaaagtga
    11881 tcagatgatc ttgcgagtag acttttctat gatccgtcgc gaatcaggtt ttatcagcgg
    11941 attagtagct gtcttgcatg atgtgacaga acaagaaaaa acagaacggg aacgtcgtga
    12001 attcgtctct aatgtttccc atgaacttcg tacaccgttg acaagtatgc gtagttatat
    12061 cgaagcattg agcgaaggtg cgtggaaaga cgaggaagta gcaccaaatt ttttgaaggt
    12121 aacgctagat gaaacagacc gaatgattcg aatgatcaat gacctcttaa atctatctcg
    12181 catggataca ggtaataccc agcttcaatt agaatatgtc aatttcaacg agttagtgaa
    12241 ttttgttctt gaccggtttg atatgatggt gggaaatcag gaaaaaaatt ataagatccg
    12301 tcgtgaattt acgcaaagag atctttgggt cgaagtagat acagataaaa tcatccaagt
    12361 agtcgataat atcatgaata acgcgatcaa gtattcacca gatgggggaa cgattacttg
    12421 tcgcttatta gaaacgcata ataacgtgat tttaagtatc accgatcaag gattggggat
    12481 tcctaaaaaa gatctgaacc gagtgttcga gcggttctat cgagtagata aagcacgtgc
    12541 aagagcacaa ggtgggacag gacttggatt agctatttct agagaagtga ttaaggcgca
    12601 tcgaggcgct atttgggcag agagcaagga aggaaaaggg tcgacttttt atatctcgct
    12661 gccatatgaa ccttatgagg aggaatggtg ggaatgagaa tctctgaaaa agtcgttcgt
    12721 ataggactca ttttgatggt tgcgttaagc atatacttct cttatgcaat ctggctgagc
    12781 ccagcaggaa aaagttcaat caacgtagat gagacaaaca ctcaaatgat cgagtctcaa
    12841 agcaatcgaa aaacttccga agtctttctt ccgcttcatg ttacatggca ccattcggga
    12901 atgatccaag aaaccaacag tgaaaatctc gtgtaccgat tgcaattgat catcgataaa
    12961 gcaagatttg gaaagctttc tgaagtagtt tcaggagaca aatcacagtt tgaacaggtg
    13021 aaaaatctga actctggttt tgagttggcc tataatgcgc cttttttgct aagagaatac
    13081 aaggaagcat ttgatttaga tttagatgtg tcgaacttgg caaaagaaag cgaaaatatg
    13141 tattttacta gaatacaatt cgatcaagga aataaaaaaa tccgattttt gaattattcg
    13201 aaccatctcg tatatgaagc aaatatttcg ttgaattgga cagatatcaa taaagaatta
    13261 acagcaaaag acacaaaatg gacagaaatg acaacagaac catcgacgat cgacacgcaa
    13321 tatgctacga aacattcgat caaactgaag aagtatagtt atatcctgtc acagcaaccc
    13381 tatacacttt ttagaaatgc ttttttccag aaaccagata gcgtgaaaaa taatgatgaa
    13441 agcaatgaat tgatttttta cggtgggaat gaaacaatgt ctatccattc ggaaacgcaa
    13501 gtagttgatt ttcgtggaga gctgccagag aaaaaagaaa atgctagtgt attttctgaa
    13561 agtttcccct atattagcaa attaggcagt tctttaggga acctcagata ttttgaccgc
    13621 agtgacaata cgatcgatta tcggatattc gtggaaggct tcccggtttt tgggacagac
    13681 agcagaggac agattggtgt ggaagttggc ggtgaaacga atggttctgt gcaagtgaat
    13741 atccaaacca gtttgaatac gattcaagtg ccgatacctt cagacgaaga agtagaactt
    13801 gcaagtacga atgaaatcat ccagcagtta aaagctaagg gagcagatag caacaagatt
    13861 ggttcagtga tcgttggcta tacatggcaa aatgtcaaag aaacgaatcg tgtcgttgat
    13921 ctcttacctg aatggtatgt gaaatataaa gataactggt attcagctaa cgaccttctg
    13981 gaaaaattac ctggattgga ggcgaattaa ttggatttca aaaaaatcga atggatattt
    14041 ttcgtcgctt ttttagggtt gaatgtgttt ttattcagca gttatcatga cgcggtatac
    14101 caagaaaaca atgtcattcg ctctaatcag actgaatcaa tccaacaacg tctggctagc
    14161 gacgatatca cttacacggg gaagatttcg tctgagaaca agcaaggcta ttatcttagc
    14221 gcggagcaga ccaatcttag cacttcatta gcgaattatc gcaaggctgg taaaagcggc
    14281 ttgctggcaa gtggtgtgtt tttagacggt gatgttttga cgaaatcttt atccagttca
    14341 gatgctgaaa agaacgtgat cgatccagat aaaattagta gttccttgga agatttttta
    14401 tctaacaaag acaatatttt attcggtaat gattatgtgt acacgaaaaa cttttctcat
    14461 ttagaagaag aaccactaga aatccaagca agccaagagt atgaaggcat cccattcaat
    14521 gatgatacag caaaaatcgt tctttcttta gaaaaaaacg ataatgatta tagcatcacg
    14581 aaatacatgc agacgcattt atctgatatc gagcagctgc gcgaaaagac ggaactgtat
    14641 acggaagagg aagcaatcaa tacattgtac atcaataaca aaataccaag aggatcaaaa
    14701 attttatggc gtcagctagc ttattcctgt attctgaaag tccgggaaaa aaatgtgtat
    14761 gttcctgttt ggcatgtagc gatcgaaaca agcgataaag tggtccaaat tgagagcatc
    14821 aacgctttca gcaatacgat cgtgacaaac aatactgtac caaaagtgga agatcaataa
    14881 aaaagcgagt ataatgaaaa aagtttacaa agtaagaagg gaagtaggag tcaaatggct
    14941 gaagaaaaac cagcttttaa aatcagtatc ctagcaagtg gaagttcggg taattcactt
    15001 tatatcgaaa gtgaaaaaaa acggcttttg gtcgatgcag gacttagcgg taaaaaaatc
    15061 acttccttga tggctcaaat tgatcggaca ccggaccaat tagatggaat acttgtgacg
    15121 catgaacatc gagatcatat acatggcgtg ggtgtcttgg ctcgtaagta tcatttggat
    15181 gtttttgcca atcagcagac ttgggaagcg atggaaccac tgattggaaa tgtaccagtg
    15241 gaacaaaaac atctatttga gatggggaaa gtcagaacat tcggcgatat cgatattgag
    15301 agtttcggcg tctcccatga cgcagcggct ccgcaatttt atcgtttcca taaagaaaat
    15361 cggtcattcg tcatgttgac ggacaccgga tattgcagtg attatatgcg aggtgtcatt
    15421 gaaaatgccg atggttattt gatggaaagt aatcatgatc ttgagatgtt acgcatggga
    15481 ccttatccgt ggaatttaaa acagcgtatt ttaggagata gaggacatct ttccaacgaa
    15541 gacggtgcac ttgtcatgac tgatgtgatc ggtaatcgga ccaaacgaat ctatcttggc
    15601 catcttagca aggaaaacaa tatgaaagaa cttgctcatt taacaatgga aaatgttttg
    15661 aaagaaaaag attttggcgt tggacatgat tttgaaatct atgatacaga tccagatgaa
    15721 gcaactgagc tattttcgat ttaaacagtt caatcaataa aaatataaaa agcagaagat
    15781 aaaatcaatc agggttggtt ttatcttcta tttttatttt tttagcatat taaaaaaata
    15841 accgtattat cgactgacta cactgatgtg ctgcttttca tgtttctcat ccagtatctc
    15901 atctactaat gcaatcgcgt aatctgccat gctgatcgta ctgtcgctag cagcgtttct
    15961 ttgtaaataa tcatcactta acaaataagt acctgtacgt ttgccatgtg gatcaaataa
    16021 agaagctgga ctgatataag tccaatgtag atttttagat tgtttcaatt ttaacaatcc
    16081 ttcgtacata ttgaaagctg ttggatagaa gggtgcattt ttaggtactt ggtcaatcat
    16141 acgagtcgtc ttttctgtat ctaagaataa cgaagaggca cccccaacga caattaagcg
    16201 tgtatcggtt ccttcaagca tctcaatcaa atgggacaaa gaagtttgat gcatttcttc
    16261 ttttcctttg ggagcgttga aagcatcaac gacaacatca aaagatgcaa gatcagtagt
    16321 tgtcaactca aataaatcct tttgtataaa cggaacatct gatggcaatt tttctggatg
    16381 acgcacgatt gccgtcacgt ctaaatctct ggacaatgct tcgtctaaaa taaatgaacc
    16441 tgcatggcca gtagctccaa tgagtgcaat tttcataaaa aacctccttt tttattcctg
    16501 tccattgtaa gcagttgctg aatattcgtc aaagaatccg atttttgaaa agctcagtgc
    16561 gcattccttt catggttcgt gatcattggg tatagttaaa ttaagaaaac tgattgataa
    16621 aaatagaagt tgaaggagat aagaaatgaa agagtggtta gatcgtgcct ttttaccaat
    16681 gataacatct gttgtgaatc ttagaccctt aactgctttg cgtaatggaa tgacttacac
    16741 gatcccaata attattgttg gctcgctgtt tttcttattt gctcattttc ctgtggatta
    16801 catcgctcag tggatagaca aaacaggatt ttctatgtat tttacacaag tataccaggc
    16861 tacatttgat gtgatagcct tttggatcgt ttttgccatt gcatacgctt atgcaaaaga
    16921 agaagacatc gatcgcttac cagtcggcat aacagccttg tctgcttatc tactgcttat
    16981 tcagccacta aatactcaga tagatactgt ctggacaggt gctgaaggga tactaggagc
    17041 agttacaact gggctcatca tcggatgggg ctatgcacgt ttagtaagac ttgcgaaagg
    17101 gtggaagcaa agagagaaga tacctgaaaa tgtttggcag tcttttactt cattgattcc
    17161 aatcattgtt atagtcactt ttacgcttat cggttcagcg gtttttcaac atttgttcag
    17221 aatgactgtt gttcaaggag tatggacata tgtccagctt cccattcaat cgttcataga
    17281 cacgttgttt ggtgtgcttt tattaggttt ttttgttccg tttttatggt tatttggtct
    17341 tcatggatct gcgatggtca atgggttggt tagtcccgtc ttgcaagcga attcattagc
    17401 aaatgcagaa atcttagcct caggacaaga gttgacggtg gcaaatggcg gacatatcgt
    17461 tactcagcaa tttctcgatc agttcatgac ggttacgggt gctggattga cgttaggggc
    17521 tgtatttttt atgacggttt ttgcgaaatc gaagaaatac agagaattag gaaatcttgc
    17581 tcttttacca gcctttttca acatcaatga atcaattatt ttttccacac cgatcgtgat
    17641 gaacccgatg atggctgttc cttttatttt cgcaccggtc gtttcaggat tgattaccta
    17701 ttccgcttta tattttggat tcgttccttt atttacagca gtccaagtcc cttggacaac
    17761 accgccgatc ctctcgggtt tcttagtagg cggagtgcct gcagcgattt tacaaggtat
    17821 cgtgttgtca attagttttt tcatttattt cccatttttg aaaaaaatag attctgcaaa
    17881 ctgcaaaaag gaaagacaga caaaaaatga tggagcagca gaaaagatga acgactaaaa
    17941 aacaaaaata ctcctgtcgc tttagattgg gaaaatcacc aatctaggat gacaggagta
    18001 tttatagtca tgtttatact agcccttttt ggacagctaa ttgaacaaga acaactaatg
    18061 tgttcattcc tacgtgtgtg atcatcgatg tccagatccg accagttgct ttgtactcta
    18121 ggctgaagaa gaaacctagg aaaaagtaga ttagcagatg accatcatta tgagcaaacg
    18181 caaacagcaa agagctgatc actgctccga cccaaaaatt tgaatatcga ctgatgatcc
    18241 ccagtatact gcggcggaat acgaactctt ccatgatggg tccagcaaca gttgtcgcca
    18301 aaataaaagc gggtgcttcc atgatcatct ggatgatatt ttgtgtattc tcggaagggg
    18361 ttgctttgcc aaacaataca gattctaaca tgacagcgat gctttgcaga atcaatgcga
    18421 caaagattcc aaccaatcca taaataacaa tctttggagc agaagctttt tttaccgctg
    18481 attcgatcgc taagggttca cgttgcttaa aataaaaaat agctaggaca acggcgccta
    18541 gtatatacat gactgcagtt gctgtgatca attcagacgt agattttaca agtcctatcg
    18601 aagctaggat caagggtgag ataaaaatta gaccatagca aaaaatgctg aggaagctat
    18661 atttttttaa agacatgtaa ttcctccttt taagggataa caagcatgca aggcaagctt
    18721 atatcgattg atttcaatta tatcataatt tcaataagca gctgtcagaa taaaatttct
    18781 tttctttgat ttaaaagctt gcaaaaaaga gggagaatga gtattattaa aaatgtaggt
    18841 tagcactcga aggttaagag tgctaacaga ttaaaaaaag agttatcgga gggatttatc
    18901 gtgttaaaac cattaggtga tcgcgtcatt atcgaagtcg caaaagaaga agaaaaaact
    18961 gtcggaggaa tcgttttagc ttctgctgct aaagagaaac cccaaactgg gacagttgtt
    19021 gcagtcggtg aaggccgttt gttagaaaac ggtgaaaaag ttcctgccgc tgtaaaagca
    19081 ggcgaccaag ttatgttcga aaaatacgcc ggtacagaag taaaatacga aggaaaagaa
    19141 tatctgatcg tcgcaggaaa agatatcatg gcgattgttg agtaaatttg gaagataaaa
    19201 aaataatcaa aaataatcaa tgaggtgaat caatcatggc aaaagaattg aaatttgcag
    19261 aagacgcacg cgcagctatg ttacgcgggg tagataaatt agcagataca gtgaaagtaa
    19321 cattaggacc taaaggccga aatgtcgtac tagaaaaatc ttacggctct ccattgatca
    19381 caaatgatgg tgtgacgatc gcaaaagaaa tcgaattaga agatcatttt gaaaacatgg
    19441 gtgcaaaact tgtatcagaa gtagcatcaa aaacaaatga tattgcagga gacggtacaa
    19501 cgacagcaac tgttctgaca caagcaatcg ttcgcgaagg tttaaaaaac gtaactgcag
    19561 gtgctaatcc tctaggcatt cgtcgcggga tcgaattggc aacaaaagca gcagtcgaag
    19621 aattgcataa tatctcaact gttgttgatt caaaagaagc cattgcacaa gtagcagctg
    19681 tttcttcggg ttctgataaa gttggacatt tgattgctga tgcaatggaa aaagttggaa
    19741 acgacggtgt catcacaatc gaagaatcaa aagggatcga aacagaacta gatgttgtag
    19801 aaggtatgca gtttgatcgt ggttacttat cacaatatat ggtaacagat aacgacaaaa
    19861 tggaagctgt tctagaaaac ccatatatct tgatcacaga caaaaaaatc tctaacatcc
    19921 aagatattct gccattgtta gaacaaatct tgcaacaatc acgtccattg ttgattattg
    19981 ctgacgatgt agatggtgaa gcattgccaa ctcttgtatt gaacaaaatc cgtggaacat
    20041 tcaacgtagt agctgtaaaa gctcctggtt tcggcgatcg tcgtaaagca atgcttgaag
    20101 atatcgctat tttgacaggt ggtacagtaa ttactgacga tctaggttta gaattgaaag
    20161 atgcaacaat cgaaaactta ggaaatgctt ctaaagttgt tgtagataaa gacaatacaa
    20221 cgatcgtaga aggttctgga gaaaaagaag caatcgaagc tcgtgtgcaa ttgatcaaaa
    20281 accaaattgc tgaaacaact tctgattttg accgcgaaaa attacaagaa cgcttagcta
    20341 aattagcagg tggtgtagct gttgttaaag ttggtgcagc tactgaaaca gaattgaaag
    20401 aattaaaatt acgcattgaa gatgcattga atgctacacg tgccgcagta gaagaaggaa
    20461 tggtctcagg tggtggtaca gctctagtga acgttatcag caaagtttct gctgtagaag
    20521 cagaaggaga cgtggcaaca ggtatcaaga tcgttgttcg tgctttagaa gaaccaattc
    20581 gtcaaatcgc tgaaaacgct ggttatgaag gatcagtgat tgttgataaa ttgaagaacg
    20641 tggaactagg cactggtttc aatgctgcta ctggagaatg ggtaaacatg gttgaagcag
    20701 gtatcgtaga cccaactaaa gtaacacgtt ctgccttgca aaatgccgct tccgtttctg
    20761 ccttgttatt aacgacagaa gcagtcgttg cagataaacc agaaccagca gcgccagcag
    20821 ctcctgcaat ggatccatca atgatgggcg gtatgatgta agaaaaagca acttgcttat
    20881 ctacaactga tcattagtga aagacagata aaaagaggct ggaacagaag cgtttaactc
    20941 caagaaataa gaagaaattc acggaaattg cttttcaaat ttttgtgaat ttcagcttat
    21001 ttccgaagga gttgcttctg cttccgctgt ttatacgtgt ttagagcgtg agacaaaagt
    21061 gttctttact tttgtctcac gctctttttt gtgtgtaatt ataatgaaaa tagttatttt
    21121 taatagttat aatattttat ctattaaaag taaata
//
LOCUS       DACEQH010000046        21114 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.046, whole genome shotgun sequence.
ACCESSION   DACEQH010000046 DACEQH010000000
VERSION     DACEQH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.046"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            474..725
                     /locus_tag="IW833_001870"
     CDS             474..725
                     /locus_tag="IW833_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307987.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="IW833_001871"
     CDS             759..1037
                     /locus_tag="IW833_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307988.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1634..1891
                     /locus_tag="IW833_001872"
     CDS             1634..1891
                     /locus_tag="IW833_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307989.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2004..2615
                     /locus_tag="IW833_001873"
     CDS             2004..2615
                     /locus_tag="IW833_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307990.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2794..3351
                     /locus_tag="IW833_001874"
     CDS             2794..3351
                     /locus_tag="IW833_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307991.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4050..4571)
                     /locus_tag="IW833_001875"
     CDS             complement(4050..4571)
                     /locus_tag="IW833_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1307992.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4770..8462
                     /gene="nifJ"
                     /locus_tag="IW833_001876"
     CDS             4770..8462
                     /gene="nifJ"
                     /locus_tag="IW833_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ1307993.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            8651..9538
                     /locus_tag="IW833_001877"
     CDS             8651..9538
                     /locus_tag="IW833_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ1307994.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            9535..10704
                     /locus_tag="IW833_001878"
     CDS             9535..10704
                     /locus_tag="IW833_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307995.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            10728..12083
                     /locus_tag="IW833_001879"
     CDS             10728..12083
                     /locus_tag="IW833_001879"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ1307996.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(12155..13960)
                     /gene="glmS"
                     /locus_tag="IW833_001880"
     CDS             complement(12155..13960)
                     /gene="glmS"
                     /locus_tag="IW833_001880"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ1307997.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            14369..14659
                     /locus_tag="IW833_001881"
     CDS             14369..14659
                     /locus_tag="IW833_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1307998.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            14672..15697
                     /gene="sppA"
                     /locus_tag="IW833_001882"
     CDS             14672..15697
                     /gene="sppA"
                     /locus_tag="IW833_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ1307999.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            15700..16359
                     /locus_tag="IW833_001883"
     CDS             15700..16359
                     /locus_tag="IW833_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ1308000.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            16516..17448
                     /locus_tag="IW833_001884"
     CDS             16516..17448
                     /locus_tag="IW833_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308001.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            17602..18891
                     /locus_tag="IW833_001885"
     CDS             17602..18891
                     /locus_tag="IW833_001885"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ1308002.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            19138..20388
                     /gene="clpX"
                     /locus_tag="IW833_001886"
     CDS             19138..20388
                     /gene="clpX"
                     /locus_tag="IW833_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ1308003.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            20471..21058
                     /locus_tag="IW833_001887"
     CDS             20471..21058
                     /locus_tag="IW833_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ1308004.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         7025 a         3605 c         4568 g         5916 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gataaagcat tttttgcatt tttataatag
     1441 tttattgata ataatgtatg agatacagta taatgattta aaaggtaacg tgttgtgacc
     1501 gaattaaatg aattaatgcc tattttttat attccttagt ggacaaaaat ggagacagtg
     1561 aagttaggag cattgtatcc tttgttgcgt agttcaaaaa agtaagcgat ttctataatg
     1621 gtcagggtaa acggtgaggg ctgtccacgt aaatcctact attgaaatca caatgatcgg
     1681 acattcaaaa ggtggtgctg aagcagcaaa ttcagtagct ataaatacga atgcgattat
     1741 atttaatcca gcgacagtaa atttatctgc ttatgaatta gactcaaaca catatacagc
     1801 aaacatggta gcttatgttg tcgagggaga tgcattaaat gttgtttttg gaggagtata
     1861 cacctattgg agaattaatc atgttagata gacaatatga tgcaccttag tggagaatga
     1921 tatctcgcat aggtaactca gtaaaaaatc aaggtatgga agcaattatc tctgcattgg
     1981 aagatcgata tagataggcg gatatgaata gtaatagggg gaataaaatg gaatacaatg
     2041 gatcaatgag aattgtacaa attatatttt ggattacatc gggaattgtg atcataggag
     2101 gagttttttt gatgttgcca agcttaatat ttcctttctt tgcattaatc tcacctaaaa
     2161 tacccgaacc ggaaatcacc tatggagaat ttccatttag gattgaatac gaactagatg
     2221 gacagattaa cataattgaa gatactgtca tagctgagtt taacgggtgt gagttcagcg
     2281 caggttctat gaaaagagaa cgaagatgga gaagtagatt agctagtggt agagaagatc
     2341 taccttttgg ggacgatcta ggaatctatt ttagtcgagg aagtgcgcaa tattatatgg
     2401 gggaaaatgt acaatcaatg tcattaaaac cacatatagc ccttagaaat ctcgaggaag
     2461 gttttaaaag agaggtagac tttatattag gagaaagtgg atacataaga actgtactta
     2521 atttcggaga agcgcaagaa gttttgacac aatacggaat taccttaatc aactgggaaa
     2581 tatctgaacc gattgttaac aattttggtg attagaaaac aattacgata aatagaaaca
     2641 agccaaatca tcagcaagta aaagctgatg atttggcctt tcttatacga gtagtaggaa
     2701 ttcatagcga atattattac gaccagtaga tattttgata ttaaaggagt attacgaact
     2761 aatatgtgag aatgtagagg tgatgtgcga ataatgagaa atttatcaaa tagaaacaaa
     2821 atattaatca ttattgttgt tatagctgtg tttcatctgg gaacgaatgc ggttttaagt
     2881 agaataatat tgggtcctaa gccaccaaga ccagaaatta cccgagggga gtttgatttt
     2941 agattagaat atgaagtaga tggggaaagg attgtgatag aagatacaat tgttgcatta
     3001 tttgatggat tttctgcaga tgcaggtagc atggcatggt atcgcacgtg gcgtctacat
     3061 ttagcaagtg atagacgatc acgaaatatc ttattagatg aattagagga tggtcgaaga
     3121 atatattatg tcccagaaag tgctaactat tctatgggag atgtacagaa aaaaagagag
     3181 cctaatccta attggtatcc atttaatggc gtaacaatag aatatccacg caataaaaca
     3241 cctgaaatag gtgcaaaatt tataagtggt ttagaagatc tgtacgatag gtttggaatc
     3301 cgtcttatta gttgggaaca cgatccacca atagaaaatc gctttgaata atcgaaatct
     3361 actaatccat ttcatcagcc agtgaatgtt gtaatggaac aatttggttt agtgttcaaa
     3421 accattgaaa tactaggctg tgatcagcaa aaatgaatct gaataaaaaa gagagcaact
     3481 attagctgag aggagttaat agttgttagc tttatacgaa tcaaagtggc aaatacagat
     3541 gcgtatgcat ttgtgtttgc catcttgcgt ttggtagacg agataggtat aagctaaaga
     3601 aacaacttgt ttaaaataaa cgacctagct caacaaatga ccatctaatc aactaacagc
     3661 atacataaaa gcaactattt ttgaagtaga attgaaatcg ggataactta aaaagtgatt
     3721 gggatactag tgagaatcac cgtacataaa tattactcgt taagggaaat tgagaaattt
     3781 tgtatgaaaa tttaagaatt agttagtatg cgcaagtcaa aaatatgaat aaattatttg
     3841 gtggagataa tttgcttagc ttgtcataag aaatgagata atctggcaga tgcaagcttt
     3901 tggacaccat ttattcggaa taaggaggct gggacagaag cgtttaactc caagaaataa
     3961 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
     4021 tttacttttg tcccacgctc cttatttttt tattttgttt ggatcaattc ttttaattcc
     4081 tctattgaaa tagattcatc aaagatttct ttacccaaga tgacagttgg aacaaatcga
     4141 atatgtgctt gctcagcttc gttgatgaca gcttggctca gctgttcgtc tttttgttct
     4201 gtcagcttta gtttatctac tgcaaaatcc gctacttctt ggagagaaag atgtttccac
     4261 tcgtcttgag tatcgaatat ttgtttgatc tctttgatcg ctttttggcc atcgctgatt
     4321 gtgagatacc ggtgcatcac gttccctcgc tgtaagcttt ctttttcttt atcgaacaat
     4381 ttgatcactc gctgcaattg cccgctttgg actgcttctt ctaatagctc ataagattct
     4441 tcaaaccatt gtcggcaata cggacaagct aagttgatga attcgaccat ttttttagga
     4501 gcattcgatg aaccataaag gattcctttt tgtgtatttg ttttcgttgc atcaatcact
     4561 gatatatcca tgttaaaaca cctctttcgc ttatagttta tacatgtctg acaaaaaaag
     4621 gcaacgattt agactgtttg tctaaaaatt agatgaccaa ttcaaataga tacatgtaaa
     4681 atatttttat aaataaaaga aaaaaatggc atgttttacg ttataatgga acagataata
     4741 agagagaata tgaacaagga tgatgaatca tgcgaaaaat gaaaacaatg gatggcaata
     4801 cggcagccgc atatatttct tatgcattta ccgagattgc tgcaatatat ccaataacac
     4861 ccagttcgac catggctgaa ttagtagatc aatggtcagc agcaggaaaa gaaaacgtat
     4921 tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc aggagcagct ggtgtggtcc
     4981 atggatcatt gaaaactggt gcactgacaa ctacatatac cgcttcacaa gggctgcttt
     5041 tgatgatacc gaatatgtac aagattgctg gggaactact gccaagtgtt ttccacgttg
     5101 ctagtcgggc agtgacgaca aatgcgttga atattttcgg agatcacgga gacgtgatgg
     5161 ctgcaagaca aacaggattt gctatgttag ctgaaagcag tgtacaagaa gtgatggatt
     5221 tagcacctgt tgcccatctt gcttctattg aaggagaagt cccttttttg aatttctttg
     5281 atggattccg tacgagtcac gagattcaaa aaatcgaagt tttggaatat gaagaattag
     5341 ctcctctgat caatcaggag aaattagctt catttcgtaa acgagggatg aatcccaatc
     5401 atccgactgt tagtggtacg aaccagaatc cagacatcca ttttcaacaa cgggaaacga
     5461 tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa atatatgaaa gagatcaacc
     5521 gattacgagg aacttcttat gacttggtga cttattacgg agcagacgat gcggaagaag
     5581 tcattgtttc tatgggatca gttgcacaga cgattgaaca aacgattgat tacttgaatg
     5641 caaacggacg gaaaacaggt tttttgaata tccatcttta tcgtcctttt ccagtggaaa
     5701 cattcgtgca aaagttgcct catacagtaa aaacaattgc tgttcttgat cgaagtaaag
     5761 aaccaggggc aggtggagaa cctctcttat tagacgttca aagtgcgtta tatgattctg
     5821 aacttcgccc ggctgttatt ggtggtcgct acggattggg gtctaaagat gtgactcccg
     5881 accaaatctc agcagttttt gatgaattga aaaaagaccc aagcatacgc aaaaaacggt
     5941 ttaccatagg gatcgtggat gatgtgactt atcaatcttt ggaaccaaag gaaagtctgg
     6001 atttgacaga accgcaaaca ttccaggcca agttttgggg atttggatct gacggaacag
     6061 ttggagcaaa taaatcagct atcaagatta ttggagatca caccgataaa tatgcgcaag
     6121 gctattttta ttatgattcg aaaaaatctg gcggtctgac tgtctctcat cttcgttttg
     6181 gagatacgcc gatacgttca gcgtatcttg tcgagcatgc ggaccttgtt gcctgtcaca
     6241 caccagctta cctgcatagc tatgacttgg tcaaaggatt gaagccaggt ggcatttttt
     6301 tattgaatac tctttggagc gacgaacaat tagaaacgca tctgccctta aaattaaaac
     6361 gttacttggc tgaaaataac attagattct atacgatcaa tgccatgaga ttggcccaag
     6421 aagtcggttt gggacgccgg atcaatacgg caatggaaac ggcttttttt aaattagctg
     6481 acatcattcc ttttgatgag gtgcttccgc tgctaaaaga agaagccctg aaaagctatg
     6541 gacataaatc aatggctatc gtagaaaaaa acatacaagc aatcgatcgg acgaccgaac
     6601 tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt ggagatgcct gaaaaaatca
     6661 ataaaaggaa cactggttat atacaagaaa tacttgaacc agtgaatgca caggaaggaa
     6721 atcaactctc tgttggtaca cttgtagcaa atggaatgac aactggcgaa atgcctttag
     6781 gaacggcttc tatggaaaag cggggcgtcg ctttagaagt acctgagtgg attagtgatc
     6841 gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca tgctgccatc cgtccatttt
     6901 tagcagatga agaggaaatg gaagaagcgc cagaaggatt tatcgtgaga gaaatgcgag
     6961 gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga agactgtaca ggttgtggtc
     7021 tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt gatgaagcct tatgaagaac
     7081 agaaagaaca agctgtgaat tgggcttttg caatgacatt aagacaaaaa gcaaatccag
     7141 tcaaaggaaa gaatactgtc ttgtctacac aattcaatca accgcttctt gaattctcgg
     7201 gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt attgacacaa atgttcggtg
     7261 atcgaatgat gatcgcgaat gcgacaggct gttcttctat ttggggcggt gcttctcctg
     7321 cttctcctta tacgaccaat gaatgtggtc aagggcctgc atggtcaaat tctttattgg
     7381 aagataatgc tgaatttggt tatggtatgc ttctagcggc acagactcgt cgcgaggctt
     7441 tggcaagttc aatcaaagaa gtattgccaa aagtatcaga agaactacga gaattgatgg
     7501 aagactggtt agtacacatg tttgagagtg aaggaacgca gcaaagagca gcaaaactgc
     7561 aagctgcgat cgaaagcgaa cgatcaattt tccctgaact ggaggatata tacaagagaa
     7621 aggatctatt cgtaaaaaac agccaatgga tgattggtgg agacggatgg gcttacgaca
     7681 ttggttttgg cgggatcgat catgtacttg ccagcggagc agacgtgaat atccttgttt
     7741 tggataacga agtctattcc aatactggcg gtcaaacctc aaaagcaaca ccagcttcgg
     7801 caatcgctaa attttcggct agtggaaagt atgcttcaaa gaaagatcta ggaatgatgg
     7861 cgatgacata tggaaatgtt tatgtggcgc aaattgcttc aggtgccaat caaatgcaaa
     7921 cgatcaaggc atttgaagaa gcagaaaagt atcctggtcc ttctctgatt attgcctata
     7981 cgccttgtat cacacatggc ttgcttggcg gaatgaaaga atcgcttaaa gaagcaaaag
     8041 aagctgtttc ttctggttat tggtcattat atcgatataa ccctacaatg gtcgaaaaaa
     8101 acaagaaccc aatgactttg gactacaaaa aacctgattt ctctgctatg cctgatttca
     8161 tgaggaaaca agttcgcttt tcctcgctag ataacgcaaa tccaaatctt gcaaaagctt
     8221 tatttgaaaa gactgtccac gacgctaaaa cacgttttta caactatgct gcactgacgg
     8281 gagaattaga aaaaatcaag aaacgacttg aaccagagga atctatcgat gtacccgtta
     8341 agactagaaa agaaaaaacg acggaccttg aagcagaaga aagaagagct gctagaagag
     8401 ctgccagagc agaacgacgt aaaaaaatgg aacaagctac aaaagaaaaa aaggaaaaat
     8461 aacagaaaaa atataatgaa taattctctg aagtttaaaa atatttattg cagagaaaaa
     8521 taaaacgcat acttgcaatt ttcttgaaaa ttatgcgttt ttctcaagaa aaatacgagc
     8581 atatcagcct ctaaaattaa aacatatgat atactagaga ggaaatctca aaagagaaga
     8641 gaggcttttt atgtcatttc aaatcgggga actttttaat ttggactatt ggcagcaaat
     8701 gttttccggt gacttgttct ctctgaattt tttggtgaac atacttgata ttctggttgt
     8761 ctggtatctt gtatacaaac tgatccaact gctgcgaggc acaaaagcga ttcagcttct
     8821 gaaaggtgta gctattttta ttgttatccg aatcgtagcg gaaatcattg gtctgcacac
     8881 cttgtcttgg ctgatgaacc aagtcattac gtacggggtg atagctgcta tcgtgatttt
     8941 tcagccggaa atccggagag gactggaaca tcttggacgc agttcgcttt tcaaacaatc
     9001 aaaaagcgaa cagcaggaag atgagaagat gatcttgtct tttgacaaag ccatccaata
     9061 tatgtccaag cgaaaaatcg gtgcgttgat cactatcgaa cgtcatactg gattagatga
     9121 gtatattgag acagggattg ctttagatgc agatatcact ggggagctgc tgatcaatat
     9181 ctttattcct aatacaccgc tgcatgacgg tgcagttatt gtaaaagaag gaaagatcgc
     9241 agtagcaagc gcctatcttc ctctatcaga aagtatgttg atccctaaag aattcgggac
     9301 tcgacaccgg gcagctgtcg gtataagcga agtcagtgac gcgattacca tcgttgtctc
     9361 tgaagaaacc ggggacgtca gcatcacgtt agataatgaa ctgatggctg gcttatccca
     9421 gcaagaatat ttggcaatct tgcgtagaga actaattgct gaagagaaaa caaaaaacaa
     9481 gaaaaacgtg attcaatctt tcctagacgg tatgactaag ggaggggatc gcaaatgatc
     9541 tcaaaagaga aacggacaag cattctttac agttttattg cattgctgtt cagtattgcg
     9601 ctgtacttca atgcaaatgg ccagtccgtt ccaaatacgc tttctggaaa tgaagcgtat
     9661 aatcaaacag ttacgggggt gccgatcaag atttcttatg attcaaagcg ctactatatc
     9721 catggatatg aaaatgcagt gactgtcaaa ctttccagtg caaaccgagt tcagttgaat
     9781 gcagaggcaa acgaagatac acggatgttc cgcgtcacag cagatatcac taagctgggc
     9841 gaaggaacac atgaagttcc gctaaaaatt caaaatttga gcagtgctgt cacagcaaag
     9901 atcgaaccat cgactattac agtaacggtt gagaaaaagg taataaaaga tttcgatgtg
     9961 gaaacgttga ttccttcaac gttcacacca tctggatatg aacttgatga tacttccgtt
    10021 agtcctaaaa aagtctctat cactacaggt gacaaaacat tggcagaaat caaacgagta
    10081 gtagctgatg tggacgcttc gatggtcaca gatgatggga ttaatagtga ggtcccaata
    10141 caggcgctga atgcagcagg ggaagtatta agtatcgtct ctgatccagt gcaagtaaca
    10201 gtgagagctg attcgatcaa gccaagcaag tcggtacgac tttatgggat acagcaagga
    10261 acaccagctg ccggggtaga aagctatgat ttcagttttt caaaactgga agcagaagta
    10321 agcggctcta gcgatttgct tgcatcgatc ggtgacagta ttgctgtacc gatcaatgtc
    10381 tcaggtatca atcatcgaac aactcgtaaa attgaaatac cagtagaaga ggggttaagt
    10441 atcaagcccg aatcagttag tgtggagatt acacccgtgt tgcaaacgtc aagcagtagc
    10501 tctgcttcga cgagcagtcg gacacaagaa aatacaagta cgagtagaag tatttcatct
    10561 gctgattcag gaacggaacc atcgacaaca accatttctt ccagttcgga atctgggaca
    10621 agcagctcga ccacaagtac agaagaaaca tcagaaacaa gcgaatcaac acaaatgagc
    10681 agaacaaatt cctcagaaaa ttaagttaaa ggagtaattt tacaactatg ggtaaatatt
    10741 ttggaacaga cggcgtacgc ggtgaagcca acaaggaact tacgccagaa cttgcattta
    10801 agctgggacg gtgcggaggt tatgtcctaa gccagcatga agaagaagga aaacgcccac
    10861 gcgtgttagt agggcgggat acaagaatct caggtcaaat gttagaagaa gcgctgattt
    10921 caggactttt atcggtgggt atcgaagtgt tccagctagg tgtgatctct acaccaggtg
    10981 ttgcttatct gacacgtttg caaaaagcaa gcgcaggggt catgatttct gcttctcata
    11041 atccagcaca agataatgga atcaaatttt tcggtgcaga tggttttaaa ctagtagatg
    11101 accaagagct agaaattgaa gcattattag atgctgaagt ggatgaattg cctcgtccat
    11161 cagctgaagg attgggaact gtagaagaat ttccagaagg cttattgaaa tattctcaat
    11221 tccttgtaca aacgatcaac ggtgaccttt ccggtttgac catttgtgtc gatgcagcaa
    11281 acggagcaac ttcaacaagt gttaatcgtt tgtttgccga tcttgaaaca gatttttata
    11341 caatgggcac aaatccagac gggttaaata tcaatgacgg cgtaggctct acccatccag
    11401 aacgtttagc tgagatggtc gttgaaaaag gagcagatgc tggactagct ttcgatggcg
    11461 atggcgatcg tattattgcg gtggatgaac ttggaaacat tgtcgacggt gataaaatca
    11521 tgtttatctg tgctaaatac ttggcagaaa aaaatcgatt gaaaaaagat acgatcgtta
    11581 cgactgtcat gagtaactta ggattccaca aagcggtgga aggaatcggt ttgaaagatg
    11641 tcgttacaca agttggtgat cgttacgttg tagaagaaat gcgcaaaaac gattataact
    11701 ttggcggcga gcagtctggg cacatgattt tcttagattt taacacaact ggagatggga
    11761 tgctttcagg aattcagtta ctgaatatca tgaaacagac aggtaagaaa ttatcagagc
    11821 ttgcagcaga ggtaacaatc tatccgcaaa aattagtgaa tatccgcgta agtgataaaa
    11881 acggtgcgat ggatgttccg gcaatcaaaa aagtgattga agaggcagaa gctgaaatgg
    11941 gatcagaagg aagaatccta gttcgtcctt ccggtacaga accattactt cgagtgatgg
    12001 cagaagcgcc tactgatgag aaagttaatt actatgtcga taaaatcgcg gcagtagtca
    12061 aagaagaaat cggactggat taagagatat tttcataaaa aataaaagaa caaggaccag
    12121 tgtcagatgt ctgacactgg tccttgttct tttattattc tactgtcact gatttcgcta
    12181 aattacgtgg tttgtcaacg tcgaatcccc gtaatagcgt tgcgtaatat gcaagtaatt
    12241 gagtcggtac gactgtggtt agagtagtta gataagggtg gacagttggt agcaccagct
    12301 ggtcactttc cttttctaat ccctctgaga caataactag tgttttagcg ccacggcttt
    12361 cgacttcttt caggtttcct cgagtatgcg caccagtcac ttcttcactg atgattccga
    12421 tcactggtgt tccttcttcg atcaaagcaa tcgttccatg tttcaattcg ccagcggcaa
    12481 aaccttctgc ctggatataa ctgatttctt tcaatttcaa agcagcttca agagaaacgt
    12541 tgtagtcagc accacgtcca atgtaaaagg catttcttgt atcgctgaga tatacttctg
    12601 ccaactcttc taaataatct ttttgggcga tgaccgattc catcgcatta gcaactaaac
    12661 tcaattcatg agtgatatcg aatacaagag aagtttcaaa ttctttttta tcaccgatag
    12721 ctttggctaa taaagttagt acagctattt gtgcagtata agctttcgtt gatgctactg
    12781 cgatttcggg tccagcatgc aatagcagtg taaaggatgc ttcccgcgaa agagtagaac
    12841 cagcaacatt tgtaatcgta agtgaagggt agccgagacg gttgattttt accagcactt
    12901 ggcggctgtc tgctgtttcg ccactttgac tcaagaaaat aaagaatggt ttttttgaaa
    12961 gaagaggtgt gttatagcca aattcactgg ctaaatgaac ctcaacagga atctgtgtca
    13021 atttttccaa agtctgttta cctgctaacc ccgcatggta actagttccg caagcaacga
    13081 tatagattcg atcgctatca gacaatgtat cgaccagctt ttgatcaaga gagacacgat
    13141 tgtcttctgt tacgtatttt tgagcgatac gacgcatgat aataggctgt tcgtcgattt
    13201 ctttcagcat ataaaaagga taggtacctt tctcgatatc tgaagcatct acttgtgctt
    13261 cgaatgaaga acgttctacg atgtcacctg caaaattttg aataataact ttttctgatg
    13321 tgacggtaac catctcttca tcttcaatct caacgaattc cttcgtatga gcaagcatag
    13381 ccattgcatc gctggcaatc acattgaatc catcgccgag tccgattaat agtggacttt
    13441 tatttttagc gacgtaaata acatcaggtg ctgtccgatc aatcaaagcg aaagcgtaag
    13501 aacctttgat gacccgcaaa gcttttaaaa acgcctcttg cgttgtagta tccttcgcga
    13561 aagtttcaat tagatgagcg ataatctcag tatcggtctc accaataaag tggtcatttg
    13621 ctaaataagc aattttaagt tcttcaaagt tttcgattac gccgttgtga acgagcacga
    13681 agcgtccact ttgggaagta tgtggatggg cgttttcttc agaaggttgc ccgtgagttg
    13741 cccatcgtgt atgaccaata cctatatttc ctgtagtatc aatatttatt ttgtttttca
    13801 aattttgaat tctgccttgt gctttgacca gataatcagt tgtgccatct gagacaaaga
    13861 taccagctga atcataacca cggtattcta atttttctag accgtcaatc aatcctggtg
    13921 ttacattttt taatccaatc attccaacaa ttccgcacat aaaaaacatc ctactttcta
    13981 taatgaattc gtatgaatgc ttccagctgc aggaaaatcc ttttgtttta tctttgcaga
    14041 cattttttgt ggattttctg tagagtgcag tccctagggc catccgccga atctttcgat
    14101 aaaccctctc ctcgtcatct gtttacacag acctggcgct atcctaaaag gtataactat
    14161 cctccttttt gttttttatc tggaaacatt taaaatcata accttttgcg aatgttcttg
    14221 tcaacaaaaa aagaataaaa aataaattgg tatatatcaa attctttgtc aaatgtcctt
    14281 tttatgtcaa aaaataatgg gtttttatct atcatctttt tcaggagttc ggtacaatag
    14341 aaagtaatcg gatagaggag gagtaaaaat ggattcaaaa attgagatca tgacactggg
    14401 aatgttaaaa aaacaattgt ccaaattcga agcatcagct ggtgtatcag atgatacgaa
    14461 aatattttta gatactggct gggatagcat ccaagaaatc gcacctgatg ccttggaagt
    14521 tgctcaagct cgtgagttta cggtagaaga tgaattaaca aaagagtcgt tttccgggta
    14581 tgcgcgggaa gaaaaagcag agcggttcga cacttctgag cagtcagaaa ctgtgatcgt
    14641 cataaaaaac ttgtattaga aaggagaaag aatgaataaa agacgttggg cagcagttct
    14701 gattgctgca ggattatttg tggtttcgtt agtttcagct agtttgacta agcctcagga
    14761 aaacgatcaa attgagggat tgaacagttg gttatatggg gatgaagaac tagctccaaa
    14821 tgtgttggaa gagggagaca gtacgcaaaa aatcgtaaaa ttatcagtag atggaacgat
    14881 tgctgcggga gggtcttcag gtttatttgc ttctgaaggt tacaatcata aaaattttat
    14941 ggaacaactg aaggcgattg aagaagatcc aacagtcaaa ggtatctttt tagaagtaaa
    15001 ttctcctggt ggcggtgtct acgaaagtgc ggaaatcgct aagaaattgg atacgattcg
    15061 taaagaacat gatatcccaa tgtatgtcag catgaaaaac atggcagcta gcggtggcta
    15121 ctatatttct gctcaagcag ataaaatctt tgcaacagaa gaaacagtga ccggctcaat
    15181 tggtgtgatc atgtcaggat tgaattattc aggacttcta gaaaaactgg gtgtggaaga
    15241 tacaacagtt aaaagcggag cgttgaaaga tatgggttca ggaacgcgac cagagacaaa
    15301 agaagaagaa gcggtgcttc aagcatatat cgacagcgca tatagtcgtt ttgtagcaat
    15361 cgtcagcgag ggacggaata aatctgaaga agaagtcaaa aaaattgctg atggaaggat
    15421 ttatgatggt gtccaagcaa aagaagtagg tttagtcgat gaattgggat ttcctgaaga
    15481 tgctttaaaa gctatacgaa aggaacaaca gcttgaagat gcggaactag tagaatattc
    15541 ttccacgcca tctgggtttg ctagtacatg gttaggaagt aaactagcag aattccaggg
    15601 attgaagcct tctgagacta gtcagatctt atccgttctt gagaatctag ggacagcgga
    15661 atcaccaaaa gcaatgtatt attacggagg tgagtaagca tgtcagagct ttcaaatgaa
    15721 aataaggaac aagaaaaaga taagcaacag cagcctgctg taggacctga ctccaacttt
    15781 tcccaaaagg tactgagctc ttttgaaaga gagcctttga cgcccgaaga aatcaaaaag
    15841 aaacaacgta aatggaaaaa gtatacggac acaagaaatg aaccgccaaa agcaaatgat
    15901 tttcctaagt atttctatgc aggttttttt atccgaatgt ttgccttctt ggtcgatctt
    15961 ttgtgtatcg gagctattac gcgtttaaca cttggtattg ccaaaaatct aggttggctt
    16021 aatttttcag attcttatct gagtctctac ggattcacag ccttacttat ttatctagcg
    16081 tattttattt tattgacgaa attcaatcat ggccaaacga ttgggaaaat gatctttggt
    16141 attcgagtca tcagctttga tgaagaagaa cttagctggt caaccgtttt agttagagaa
    16201 ggtgcttgcc ggttcattct aaaatttcct ttgctgatgt tagggtatct tccagcagtc
    16261 tttaatcaga aaaagcaaca tctgggcgat tttttcacta atacaagtgt tgttacgatc
    16321 aatctgatca aagcgttcaa ccaggaagtc aacagctgat gagcaataaa aagcagtaac
    16381 agttgaaagc accttctaaa agtcagatct aaaatctagc ttttagaggg tgcttcagtt
    16441 atttttaatt gtcatgcttt ttttgttttg ctactattga acatgagctg aatatgagca
    16501 agaggaggac actgcgtgaa gccgattgat tttccagaaa aatatgagaa tttaatgcga
    16561 gtggcacaac aagcactagc aaaccagcaa tatcagcagg caaaagaatt atttcaacga
    16621 gcttacgagt tgaaagaaag ctttgaggct aatagcctgc ttgttttttg tctttacgag
    16681 ttggacgaga aaaaagaagc attgaagcaa gcactcttgc atgaaaaaca gtatcttcaa
    16741 aatgaggagt ttgctgaatt ttattttgac ttgttaatca gtacacaaga ttttttatat
    16801 gctagaaaat tgatagcaag tacagatttt tatgaagctt tcgagcagcg ggtcattgaa
    16861 aaaatccaat ttgcagaaga attatccgga caaatggaga ggcagaagat caaggcactc
    16921 cacaataaat cggaggaact tccttcgttg gaacctgcac gtcagcttgc gtctattgat
    16981 gagatcgaac agcttcctta tcatgagttt atccaaacag cgagtaaact tattgtatcg
    17041 ccagaggtcc atattttagc aagggcaaaa ttactggaga cacttaggca attgaacgaa
    17101 tgccatcctg ttttctattt gacgattgaa gaaaagttag tcaaagtcat accaaaagat
    17161 ctgccaaagc cgcagcagca ggcaagttac cagcaattat gtgtattttc agatcgttac
    17221 gggaatgaag atgcgctgct tagttccgtg ttaaaagaag aattcacgtt gcaatctgca
    17281 attgtttatc ctgtctatga tacatacatc gaagacccga agcgctggtt tcaactgact
    17341 gtagaggcgt gtacaggaaa aacgatgtat gaaggtacag aagatgaaaa acaagatttt
    17401 ttaaaaaaaa gagaaaaaat tttgcagcaa atgatcttct tccattagat tttatcagtg
    17461 aaaaagataa aaatgttatg aaaaacaagg attctatcca ttcaatatgt ttacaatttg
    17521 aaagggttag tgtactattt aaaggtatgc atgaaacgtt tttgatgtat aaaatgaatt
    17581 tctaaactcg gagggaatta aatgactgca acatttgaaa aaaaaggcac gaatgatggt
    17641 gttttaaaat ttacgattgc gcaagacgaa atccaaaaag gattaacaac tgcgtttaat
    17701 aaagtgaaaa aaagcttgaa cgtacctgga ttccgtaaag ggaaagtttc tcgtcaagta
    17761 ttcaaccgta tgtacggtga agaagcttta tatgaagatg cattgaatgc tgtattacca
    17821 gaagcttatg aagctgctgt caaagaagct ggcatcgatc ctgtttctca accaaaaatc
    17881 gatgttgaaa gcatggaaaa aggtcaagac tgggtaatca ctgctgaagt aacagtgaaa
    17941 ccagaagtaa aattaggaca atataaagat ctagttgttg aaaaacaaga ccgtgaagta
    18001 acagatgctg acgtggatgc acgtatcaaa cgtgaacaag aagcacaagc tgaattagtt
    18061 atcaaagaag atgctgcagc tgaaaatggc gatacagtag taatcgactt cgaaggcttt
    18121 gtagatggcg aagcgtttga tggcggaaaa ggcgaaaatt actcacttga attaggttca
    18181 ggctcattca ttccaggttt tgaagatcaa ttagttggtc ataaatcagg tgaaagtcta
    18241 gatgtaaatg taactttccc agaagattat caagcagaag atcttgctgg aaaagaagca
    18301 gtcttcaaag tgacaatcca tgaagtaaaa gcaaaagaag tgcctgaatt agatgacgaa
    18361 ttcgcaaaag atgttgatga cgaagtatca acattagacg aattgaaaga aaaatatcgc
    18421 aaagaattag tagaaacaaa agaaaaagca gcggacgatg caaaagatga agcagcgatc
    18481 cgtatggctg ttgaaaacgc agaaatcgtt gaattgccac acgtgatggt tcatgatgaa
    18541 gtacatcgtt caatggacga attcttgaac aacatgcaac gtcaaggtat ctctcctgaa
    18601 atgtactatc aattaactgg ttctacagaa gaagacttgc ataaacaatt cgaaggtgaa
    18661 gcagaaatgc gcacaagaac aaatcttgtg atcgaagcaa ttgcagctgc tgaaaatctt
    18721 caagcaacag aagaagaaat caatgcagaa atccaagaat tatcagaaac atacaacatg
    18781 ccaatcgaac aaatcaaacg cgtgttgaca gaagatatgc tggaacatga tgtgacaatg
    18841 aaaaaagcaa tcgaattgat cacaggtaca gcgacagaag cgaaagaata gaaatcaagt
    18901 aataaatcat ttctgaagaa gctacttcta gaaaattgtt tattcgaaat aagggactgt
    18961 gacacgattt tgtcacagtt ccttattttt tgttaagagt aaaaaatgca aaggtatagg
    19021 tcagttgatc ctttgttaaa cactttttct ttttactaaa ctatgttatg atagtaaaag
    19081 actgtaaagt ctttgatagt ttcttgcata ctttttgcaa gttaaggggt gaagaccatg
    19141 tatgacaatc caagtagcaa tgagaccgta cgctgttctt tttgcgggaa aacccaagaa
    19201 gaagtgaaaa agatcgtagc tggtccaggg gtctacatct gtaatgaatg tatcgaccta
    19261 tgtaaagaaa tcattgatga agaattttat gaagaagctg tacgcgaatt cactgaagtc
    19321 cctaaaccac tagaaattct tgaagtattg aataactacg tgattggaca agatcgcgca
    19381 aaacgctcgt tagctgttgc cgtatacaat cactataaac gtgtcaatca gcaagcacaa
    19441 gaaaacacag atgatgttga actgcaaaaa agcaatatct gcttgatcgg acctacaggg
    19501 tctggtaaaa catttttagc acaaacatta gcaaaaacat tgaatgtacc ttttgcaatc
    19561 gcagatgcga caagtttgac agaagctggt tatgtggggg aagatgtaga aaacattttg
    19621 cttaaattgc ttcaatctgc tgattacaat gtggaacttg cagaaaaagg gatcatctat
    19681 attgatgaga tcgataaaat tgcgcgtaaa agcgaaaatg tatctattac tcgtgacgtt
    19741 tccggcgaag gtgtccaaca agccttgctg aaaattctag aaggaactgt tgcaagtgtg
    19801 ccacctcagg gcggacgtaa acatccacat caagaattta ttcagattga tacgacgaac
    19861 atattgttca tcgtaggtgg tgcatttgat ggcatcgaaa cgattgtgaa gaatcgtatg
    19921 ggcgaaaaga cgattggatt tggtacacaa aaccaaaaac tttctgaaga cgaaagtgtc
    19981 atgcagcata tcattcctga agatttacta aaattcggat tgatccctga gtttatcgga
    20041 cgattacctg ttatggcagc tttagagaaa ttgacaacag atgatctggt acggatcttg
    20101 actgagccga aaaatgcgct agtcaaacaa tatcaaaaac ttctttctct agataatacc
    20161 gaacttgaat tcgaacctga agcgttgaga gccattgcga aaaaagcaat cgaacgcaat
    20221 accggtgctc gaggacttcg ttccatcatt gaagaaatca tgatggatgt gatgtttgat
    20281 atcccttcaa atgaaaacat cgaaaaagtg attatcacaa aagaagcagc tgaattgtct
    20341 ggtgagccaa cagtaatcta taaagattca gagaaaaaag cgggttgatg aaaagaggct
    20401 gccgtaaaag tcattttggc ttttgcgaca gccgcttgct ttattcatca ccttgttaag
    20461 gagagagacc atgaaagtcc atcaagctga aattatcata agtgcagtgg caccaaaaca
    20521 atatccagat acacaattgc cagaaatagc tctggcagga cgatcaaatg tagggaaatc
    20581 ttcttttatc aacacattga ttgatcgaaa gaatttagcc cgtacttccg gtaagccagg
    20641 aaagacccag acattgaatt tttatttgat cgagaacgca ttacattttg tcgatgttcc
    20701 gggatatggg tatgcaaaag tatccaaaac tgagcgagca aaatggggaa agatgatcga
    20761 gacatattta acgactagag accagctacg tgcggtcgtt tctttagtag atttgcgtca
    20821 taagccttct caagaagata tccagatgta tcaatttttg aagtattatg atatccctgt
    20881 aattgttgtt gccactaagg cagataagat ccctcgtggt aaatggaaca agcatgagag
    20941 cgaaatcaaa aaagcgttgg attttgatcg caatgacacg tttattctct tttcttccgt
    21001 tacaaaacag ggaaaagaag aagcgtgggc agcaatcgaa caagcaattg aatcataaaa
    21061 agaataaaaa agacttacca gtcaaactct ttcataaaag tttggctggt aagt
//
LOCUS       DACEQH010000047        20664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.047, whole genome shotgun sequence.
ACCESSION   DACEQH010000047 DACEQH010000000
VERSION     DACEQH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.047"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            168..362
                     /locus_tag="IW833_001888"
     CDS             168..362
                     /locus_tag="IW833_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308005.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IW833_001889"
     CDS             631..930
                     /locus_tag="IW833_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308006.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IW833_001890"
     CDS             complement(1027..1461)
                     /locus_tag="IW833_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1308007.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IW833_001891"
     CDS             complement(1693..2403)
                     /locus_tag="IW833_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1308008.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IW833_001892"
     CDS             complement(2393..3253)
                     /locus_tag="IW833_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ1308009.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IW833_001893"
     CDS             complement(3258..3896)
                     /locus_tag="IW833_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ1308010.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IW833_001894"
     CDS             complement(3911..4210)
                     /locus_tag="IW833_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1308011.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IW833_001895"
     CDS             complement(4253..5737)
                     /locus_tag="IW833_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1308012.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IW833_001896"
     CDS             complement(5757..6218)
                     /locus_tag="IW833_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1308013.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IW833_001897"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IW833_001897"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ1308014.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IW833_001898"
     CDS             7521..8291
                     /locus_tag="IW833_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1308015.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IW833_001899"
     CDS             complement(8564..9391)
                     /locus_tag="IW833_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1308016.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IW833_001900"
     CDS             9959..11194
                     /locus_tag="IW833_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1308017.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IW833_001901"
     CDS             complement(12193..12453)
                     /locus_tag="IW833_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1308018.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="IW833_001902"
     CDS             complement(12480..13781)
                     /locus_tag="IW833_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1308019.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="IW833_001903"
     CDS             complement(14073..14531)
                     /locus_tag="IW833_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1308020.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15869)
                     /locus_tag="IW833_001904"
     CDS             complement(14544..15869)
                     /locus_tag="IW833_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ1308021.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17298)
                     /locus_tag="IW833_001905"
     CDS             complement(15886..17298)
                     /locus_tag="IW833_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1308022.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="IW833_001906"
     CDS             complement(17315..18079)
                     /locus_tag="IW833_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ1308023.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="IW833_001907"
     CDS             complement(18183..19058)
                     /locus_tag="IW833_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1308024.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="IW833_001908"
     tRNA            complement(19313..19397)
                     /locus_tag="IW833_001908"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
     gene            complement(19415..19486)
                     /locus_tag="IW833_001909"
     tRNA            complement(19415..19486)
                     /locus_tag="IW833_001909"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19452..19454),aa:Gln,seq:ttg)
     gene            complement(19496..19568)
                     /locus_tag="IW833_001910"
     tRNA            complement(19496..19568)
                     /locus_tag="IW833_001910"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19533..19535),aa:His,seq:gtg)
     gene            complement(19630..19714)
                     /locus_tag="IW833_001911"
     tRNA            complement(19630..19714)
                     /locus_tag="IW833_001911"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19678..19680),aa:Leu,seq:caa)
     gene            complement(19735..19805)
                     /locus_tag="IW833_001912"
     tRNA            complement(19735..19805)
                     /locus_tag="IW833_001912"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19771..19773),aa:Cys,seq:gca)
     gene            complement(19851..19922)
                     /locus_tag="IW833_001913"
     tRNA            complement(19851..19922)
                     /locus_tag="IW833_001913"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19888..19890),aa:Gln,seq:ttg)
     gene            complement(19934..20006)
                     /locus_tag="IW833_001914"
     tRNA            complement(19934..20006)
                     /locus_tag="IW833_001914"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19971..19973),aa:His,seq:gtg)
     gene            complement(20009..20082)
                     /locus_tag="IW833_001915"
     tRNA            complement(20009..20082)
                     /locus_tag="IW833_001915"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20048..20050),aa:Trp,seq:cca)
     gene            complement(20089..20169)
                     /locus_tag="IW833_001916"
     tRNA            complement(20089..20169)
                     /locus_tag="IW833_001916"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20133..20135),aa:Tyr,seq:gta)
     gene            complement(20171..20246)
                     /locus_tag="IW833_001917"
     tRNA            complement(20171..20246)
                     /locus_tag="IW833_001917"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20211..20213),aa:Phe,seq:gaa)
     gene            complement(20282..20354)
                     /locus_tag="IW833_001918"
     tRNA            complement(20282..20354)
                     /locus_tag="IW833_001918"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20319..20321),aa:Asp,seq:gtc)
     gene            complement(20364..20436)
                     /locus_tag="IW833_001919"
     tRNA            complement(20364..20436)
                     /locus_tag="IW833_001919"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20401..20403),aa:Val,seq:tac)
     gene            complement(20452..20523)
                     /locus_tag="IW833_001920"
     tRNA            complement(20452..20523)
                     /locus_tag="IW833_001920"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20488..20490),aa:Glu,seq:ttc)
     gene            complement(20538..20627)
                     /locus_tag="IW833_001921"
     tRNA            complement(20538..20627)
                     /locus_tag="IW833_001921"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20591..20593),aa:Ser,seq:gga)
BASE COUNT         6532 a         4319 c         3274 g         6539 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttaacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
    19441 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaagggcga
    19501 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
    19561 acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat
    19621 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
    19681 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
    19741 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
    19801 ccgccattat actattatca atctataaaa taaagaataa attatagaaa ctggggtagc
    19861 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
    19921 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
    19981 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
    20041 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
    20101 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
    20161 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
    20221 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
    20281 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
    20341 tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
    20401 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
    20461 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
    20521 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
    20581 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
    20641 ctcaatactc tttctcaaaa tgag
//
LOCUS       DACEQH010000048        20523 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.048, whole genome shotgun sequence.
ACCESSION   DACEQH010000048 DACEQH010000000
VERSION     DACEQH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.048"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..111
                     /locus_tag="IW833_001922"
     CDS             <1..111
                     /locus_tag="IW833_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1308025.1"
                     /translation="ISREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(569..844)
                     /locus_tag="IW833_001923"
     CDS             complement(569..844)
                     /locus_tag="IW833_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308026.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            1298..1504
                     /locus_tag="IW833_001924"
     CDS             1298..1504
                     /locus_tag="IW833_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308027.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            1700..1867
                     /locus_tag="IW833_001925"
     CDS             1700..1867
                     /locus_tag="IW833_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1308028.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            1893..2237
                     /locus_tag="IW833_001926"
     CDS             1893..2237
                     /locus_tag="IW833_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1308029.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            2242..2523
                     /locus_tag="IW833_001927"
     CDS             2242..2523
                     /locus_tag="IW833_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308030.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            2626..2940
                     /locus_tag="IW833_001928"
     CDS             2626..2940
                     /locus_tag="IW833_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ1308031.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            2918..4612
                     /locus_tag="IW833_001929"
     CDS             2918..4612
                     /locus_tag="IW833_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1308032.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            4632..5810
                     /locus_tag="IW833_001930"
     CDS             4632..5810
                     /locus_tag="IW833_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1308033.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            5773..6459
                     /locus_tag="IW833_001931"
     CDS             5773..6459
                     /locus_tag="IW833_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ1308034.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            6459..7619
                     /locus_tag="IW833_001932"
     CDS             6459..7619
                     /locus_tag="IW833_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1308035.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            7629..8504
                     /locus_tag="IW833_001933"
     CDS             7629..8504
                     /locus_tag="IW833_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308036.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            8501..8812
                     /locus_tag="IW833_001934"
     CDS             8501..8812
                     /locus_tag="IW833_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308037.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            8802..9155
                     /locus_tag="IW833_001935"
     CDS             8802..9155
                     /locus_tag="IW833_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1308038.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            9145..9546
                     /locus_tag="IW833_001936"
     CDS             9145..9546
                     /locus_tag="IW833_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308039.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            9539..9943
                     /locus_tag="IW833_001937"
     CDS             9539..9943
                     /locus_tag="IW833_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308040.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            9955..10563
                     /locus_tag="IW833_001938"
     CDS             9955..10563
                     /locus_tag="IW833_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1308041.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            10583..10945
                     /locus_tag="IW833_001939"
     CDS             10583..10945
                     /locus_tag="IW833_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308042.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            10948..11130
                     /locus_tag="IW833_001940"
     CDS             10948..11130
                     /locus_tag="IW833_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308043.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            11147..14578
                     /locus_tag="IW833_001941"
     CDS             11147..14578
                     /locus_tag="IW833_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ1308044.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVKKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            14629..15366
                     /locus_tag="IW833_001942"
     CDS             14629..15366
                     /locus_tag="IW833_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1308045.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            15376..17667
                     /locus_tag="IW833_001943"
     CDS             15376..17667
                     /locus_tag="IW833_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308046.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            17691..19817
                     /locus_tag="IW833_001944"
     CDS             17691..19817
                     /locus_tag="IW833_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308047.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19980..20426
                     /locus_tag="IW833_001945"
     CDS             19980..20426
                     /locus_tag="IW833_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308048.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            20428..>20523
                     /locus_tag="IW833_001946"
     CDS             20428..>20523
                     /locus_tag="IW833_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1308049.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT         7228 a         3081 c         4292 g         5922 t
ORIGIN      
        1 attagccgtg aagtgggtta ttcagaaaga agtatacaac ggatgaagtc ggaagctcta
       61 atagagtttg cagaagcata taaacacgga aaaataattg cttataaata atttggcggt
      121 tttttggcgg aatgatggcg gtttttagac atttaccagt gttattatgg tagtgtcgaa
      181 agattaggaa acaggatcga caaaataaac gaagggaggc aatctccctc atcgttttaa
      241 acttcttctt agatagacag caactaatta tgaagaaaag gatgtgaatt caactcctcc
      301 taaattcttc atcggttgct gtttattttg attagacagc agcactcaca aaactaaaca
      361 tgttcttgtc gtctttcaac tgctgctgtc tattaattta tgtattggag ggaagaaaaa
      421 tgaaattatc agtagaaggc actccagaag aaatacaaga attgctccaa gctattggta
      481 gtagcaagga gcaaaaggta agtgtttcta attcagatat tgacggtatt tgcaattgtt
      541 taaacagaga aaaaatatct aatccttact aagagaaaat atgttcgtta atgaatttcc
      601 aattttcctc tgtagctcta ccttgataat ttttttgcag ttcacagatg aataaggaat
      661 cgttattatc aagtactgta tttaattttt cacacatttg atttggattc aaattagttt
      721 taactaacca aaaagatttt tgtagcttta tatatgcccc aaattctttt attatttcaa
      781 atactttatc atacttttgc cctgggttat taagatcata actaatcatg tagggtttat
      841 acatgttgtc ttgcacatcc ttgttttata tttcagcgga ccactcgctg ataaataaaa
      901 ttatacgctt agtatttatt ttcacaatat taatttatcg taataacttt tgagtaaata
      961 gaacaaaaaa cctgcactag tttccgctag tacaggcagt gactatatcg gttgataatc
     1021 tagcatatat taaaaataat tgcaagaaaa gaaatttata gtggtttata ccaaattatc
     1081 aaagcaatgt tattttgttg ctgtctattg ttttttaatt attcacacga taactaaagg
     1141 tgggtgaaga gaaatgattc cattaataat ttcaattttt gcgctctgtc ttaatgtcta
     1201 tatgattgga tttaaaaatg ggcaaaataa aaaatagtag cagccaagaa taattttata
     1261 gtgtcactgt ggcggaaagg gtagacgcta agcatgtgtg ctaggtcaat gcttcggcaa
     1321 ccatgcaagg ttcgattcct tgccagcgac attaaatgcc tatgacggtt acgactaccg
     1381 aaaaaagatc gttaagaagc tatacggtgc tacgtacggc aatgtagtaa gtgtgctatc
     1441 tgtacaccac caagcttcgg tcactgtggc ggaagtagaa gacgcagcgg taaatggcga
     1501 gtagcctcgt gagagcctgg taagttctcg tgagtggtgc aatcccactc cagcgacttt
     1561 aagcaaccga ggcatcggcg gtttaaaaat ataggggtgc gcaatttcgt acgcgttttg
     1621 tgcatcgtgc aagttactat tacatattag atcactcttt gagtggtctt tttatttttg
     1681 cacaaaggag gaaacaacaa tgtatagacc acaatactta gaacagaagt atgaagtaat
     1741 cactgttcat aaaggtaatg gcgaaaaagt atatgagtat agaagaccaa taaagagcga
     1801 tacatataaa cgaaaggaaa acaatgaagt tattccattg tatggcaaaa gaatagctaa
     1861 gcattaaata agattgcgaa aggagacgga acatgaccga ggaattctat agatggctat
     1921 tacagttgac aagagaagat cgtttggtta agttctatca gtctcctaaa tggcgcaggc
     1981 ttagagagaa ggcgatgaaa cgagatcact atgaatgcca agagtgtaga agactaggta
     2041 agtatcatag agtagagaac gttcatcata taaaggaagt caaggataga cctgacttag
     2101 ctttagattt agataatctt atttgtttat gtgttgaaca tcataatgaa gttcatggca
     2161 gatatcttac agcattagat aaacaagaga agaagataga aagctttgct aacttcgatg
     2221 caagtgaaag gtggtaagtg catgatcatc aatgataatg gcagagagta tgatacagaa
     2281 aagattgaag agtattcatc ttatactcag ggattaatta aacgtttgat atacgttcgc
     2341 tatgtaggta ttagggatct gttatcagat aactgttgta gtaaatacaa agtgaatcaa
     2401 gtaagagaag cgttgaataa agataataac gtcgaaagaa taaaaaatgt ttttggatat
     2461 agtattgaag agattaatta ttacattgac ttcgctgaag ctttcattcc gatggtgaga
     2521 taaccccccc ttaaaataaa tcgcaaattt tttgggggtg atgaaacgga gggggctgtc
     2581 aggaaaagag attttttcga actttatcat gaaaggaggg ctaaaatgtt taaaaacgaa
     2641 ttgtctcaaa atcgctacag agaaaaatta cgccgctctt taataagcca attggaaagt
     2701 cagaaaacaa atattgagcc attcttagat aatgttgatc gttatatcag tttatgggaa
     2761 acggcgatat cactggaaga agatatatcc gagaacggca ttagactgga gaatggtaaa
     2821 aagaatgaat cagtagcgtt gcttgtttct gtcaacaaac aaatgggatt gatgttggat
     2881 aaacttgcca ttactcctga attggtaggt gaagcaaatg aatcaattcc tgagttataa
     2941 gcatattgaa aattggttca aagctataga agaaggcact atcaaggtat gcaaagagca
     3001 attattgcta aaaaattatc tagaagaaag agtctttact agagaagata tttacttcga
     3061 taagcagatg gtagaggatt caatcaatat accagcacaa tactttccat tcgaattaat
     3121 tccgtgggaa aaatttctac aatgttttat ttatggtgtt cgatggaaaa aagataaaac
     3181 actagtgttc aatagatatc tttcattaat gggacgtggt aatggtaaaa ctggttttgc
     3241 ttcttggaac aacttctttc tactaaccgc taaacacggt attaaaaatt atgatattga
     3301 tatctatgcc aataatgaaa gccaagcaaa gactagtttt gatgatgtat ttaaagtaat
     3361 taaagatcat cctgatttag ataaaaaagt atttaaagct acgaaggaag ttattcaaaa
     3421 tatcgctaca aacagcaaac ttcgttataa cacggcaaat gctagaacaa aagatgggaa
     3481 gcgaccaggt gcaaaccgct ttgatgaaat tcacgaaaat gaagattatt caatgataaa
     3541 tgtggctact tctggtggtg gtaaaattcg agattataga gaattttatg atacaactaa
     3601 tggtcatgtt cgtggtggtc cgcttgatga cattatagaa gaatcaaaaa tgattctttc
     3661 tggagaactt ggaattgaca aggatggagc agaattttct agtttgtttc catttatttg
     3721 tcgcttggat aacgataatg aagttgatga tcccgacatg tgggaaaaag cttgtccaac
     3781 tattaattac aatgcagatt taaaacggaa aatgtttcaa gaatactctc aaatgcaacg
     3841 taatgctggt ttaagactta cgttcatgac caaacgaatg aacagaccta tggaagatac
     3901 acgatttgct gttgcttcat atgatgatgt tctgcatacg aaagaaaaag aatttcctga
     3961 aaaaatggat gaagtgatag gaacagtcga ttttgctgat agacgagatt ttgccagcgt
     4021 tgggttgcta ggaaaatacg ataaagatgt ttattttaca caacatactt ttatccacga
     4081 atcagccctt cgattacaaa acatcaaacg agaggttata gatatttcta tagatcaagg
     4141 aaaatcacag atcgttcatg gaaaaaatat agaagctgat tatattgtag gttggtttct
     4201 tgaaatgagt aataaatatt atattaaaaa aatcgctatg gatatgtacc gtgcaaaaat
     4261 attgaagccc gctttagaag aagcaggttt tactgtggaa attgttcgaa gcggatctgt
     4321 tacacatggt atgttaaaag atctggttga tgaccttttt attaatcaac gtttattttt
     4381 tggtgacgat gcgattatgc gttggtattg catgaatgta tatgaagagc atatttctaa
     4441 tggaaatata cgctatgaaa aaatagaacc tgaaactaga aaaacggatg gctttttttc
     4501 attccttcat ggtttgaatt ttttagatga tatttatgat tctgctcctg taacagtcac
     4561 aaatagctca gtagaaaata caggaactgg atttactcct ctagtattct aacttgaaag
     4621 gaggtgagaa agtggggatt tttcaaaagg cggtaggata cttcacaaaa aaagcaacgg
     4681 ttcctttaga agaatacttt tgtaaattgc aagttgattt tgtgtatcga aaatttgcga
     4741 ttgaaacttg tattgatttg attgcaaatg cgatgagtaa agcggaattc aagtcatatg
     4801 aagatggaaa aaataaaaag aatgatcttt actataggct gaatgtagct cctaataaga
     4861 aaaataatgc aacagaattt agaaaaaaac tgatcaggag attaatattc tacaatgaag
     4921 tattgatcgt ttctccgtct aataattcta gcgaaatatt tattgcggat agttgggatg
     4981 tcacagaata tgcattgaaa gatgatgtgt tttctcaagt gcaaattaac aacatagtcc
     5041 ttgatagaga atttctagaa agtgatgtta tctatataaa atacgcagat caacaaatta
     5101 ggcaactagt cgatgcgtat tatcaagcgt atgggaaact catttctagt gccatgaatg
     5161 tttacaagcg ctctaacgct cgtagatacg tactgaaagg gaatttattc cgaccgcaag
     5221 acaatacaac acaagatcaa atcaataaaa tgatgacatc acaatttaag gcttttatgg
     5281 aagctgataa tgcaggtgcg gtatttcaat tacaaaatga gtacacatta gaagatttca
     5341 gcggaaactt ccaaagcaat tcaagagata taaaaaactt aatagacgac atctttgaga
     5401 tgacagcagc agcgtttcac gttccgaaaa acctactaaa gggagacatg agtgggttat
     5461 cggatcaagt ggacgctttt ttaatgttcg aaatcatacc gattgctgaa cttattcagg
     5521 atgcgtttaa cgctagtctc tatgaagtag aagaatactt gtcagggaat tttgtacgtg
     5581 tggatacaac tatgatcaag attactagct tcaaagattt ggttgacgct attgatgtag
     5641 gcattagaaa tggagtattt acaatcaacg aaggaagaga acgcgttgga aatgatcgct
     5701 ctgataaggc gatggcagat gaaatattta taactaaaaa caatcaacaa gtatcgaaag
     5761 gaggtgaggc gaatgacgac aatgaaaaca tttctagcag taaagaatga aggcgcagta
     5821 ccgcaaattt ttattcaggg atttattggt tctagttggt tctttgaagg gaatactgac
     5881 aagggaatca aaaatatttt ggatagtcta ggtgatcaag aagaaattga agtagtaatt
     5941 aattcaaacg gtggagacgt atttcaaggg attgctattg ggaacttact taagtcaaat
     6001 aaagcaaaag ttaacgttgt gattaacggc ttagccgcta gtgctgcttc aattatcgca
     6061 atggctggcg atactataaa aatctacaac aatgcacaat tgatgattca ccgcgcttcc
     6121 acatacggag aaggaaatgt cgatgacttc cgtacgattg ctgaccaact ggaatcaatt
     6181 gataaatcgg taaaggcttc atataaaaca cgattcaatg gcacagatga agcattgcaa
     6241 gaacttcttg aaaaagaatc gtttatggat gcagaaacag ctttgagtta tggattggtc
     6301 gatgaaatta tcgatgcaga aaatagctca ggtactgaag ctaaaaaaga acaaagcgtt
     6361 gaagaaattt tgaatgacgt taaagaaaaa agagcagaaa aaattgctgc atttacagca
     6421 gcattaaata aaacatttgg acaaggagat gtaaaataat gacagttaaa aatttaaaag
     6481 gtgtaacagc tgcaagcgac caattgatga aagcttttaa agatggtaac gaagaatctt
     6541 ttagcgcagc tatggtaagc ttatctaagg aaattcagga taaaatttta gaagaagcaa
     6601 cagcaaaaaa tcaagatcaa ttagtattaa tgaaccgtgg tcagcgtgta ttaactacac
     6661 aagaaacaaa attctataac gaagtagtga aaaacgaagg ttttgcaggg gtcgaagaat
     6721 tagtgccagc tactgtattt gaacgtgtat ttgaagattt agaacaatct catccactat
     6781 tgcaaaaaat tacttttgtt aacacaactg gtgtaacaga atggattgtg tcacgtggag
     6841 tcaatccagc atggtggggt aaactttgcg aagctgttaa aaaagtttta gataatggct
     6901 ttgatgtaat taacatgaag cagttcaagc tatcaggtta tattcctgta tgtaaggcaa
     6961 tgcttgattt aggtccagta tggttagatc gttatgtccg tactgtttta gtagaatcat
     7021 tgagaattgc attagaacaa gcaattgttg atggtactgg taaagatatg ccagtcggaa
     7081 tgatgcgtga catgagcaaa caaactagcg gagaatatgc tgaaaaaaaa gcagaaccta
     7141 ttacagcttt agatgctgta actatgggcg gtttgatggc gcgactatca aaattcaata
     7201 tcgaaggtgt gaatgatccg atttatcgta atgtgaatcc ttctgatgtg gtcctaattg
     7261 tgaatccaac tgattactgg tctaaagtat tcccagctaa gactgtacta actgctaatg
     7321 gagaatacgt acaagtattg ccagtaccag tttcagattt gcagtcaacg gctgtgccag
     7381 aaggaaaagc agttattggg gtagcctcag attacttcat gggtgtagga tctacactaa
     7441 aaattgaagc ttcagatgaa taccattttg ttgaagacga acgcatttat ctagctaaac
     7501 aatatgcaaa cggtcaacct aaacgtaacg atagtttcat tgtattagat attagcgctt
     7561 tgggaactac tactacaact acaaaaccaa caaccacaac aactacaaca caagcgtagg
     7621 tgatcagaat gaagtatatt ctttgtcagc cggcaatcaa tcggtttaaa tgggagcttg
     7681 aagtttgttt aactaatctg aagaaactag gaatcaaaga tatcgtattg cttttcagca
     7741 gacacgatga tcagattcct attttttttg agaaggaata tggtgttgaa gttcatgtgt
     7801 acgatgatct gcgggacgac aaagagtata ttccttcgat taaaccatat ttatggtgga
     7861 aatatttaga agaagatcat tcgcgtgagg acgaccgata tttctatatc gattcggatg
     7921 tcattttcaa taaaagaatt aatttgcgca aattgccttc taaagatgat gtttggtatt
     7981 gtagcgactg ctgtagttat ctaagtcttg attatattag aagctgtgaa aacggagaaa
     8041 atattctaaa agatatggca aacattgtaa atgttacagt agaatctttg gaaactataa
     8101 acactaattc aggaggcgca cagtgggtta ttaaccgtcc taaagcgaat tattggaaaa
     8161 aggtttatct ggattctaat cggctatatc gctaccttag agggcaaaaa acaaatatac
     8221 aaatctggac agccgagatg tgggcacagc tttggaacat gatgtatttc aatattggtc
     8281 ctaaagttca cgaggaatta gacttttgtt ttgctactga tccaatagaa aaagttaaag
     8341 aagtaaaaat cttacacaat gctggagtaa caacaaacga tgaagattta tttttcaaag
     8401 ggagatacgt gacttctacg ccttttgatg aagatttatc atttgtaaac aagaaaaaat
     8461 gctcttacgc atatgttaaa gcaattaagg cggtggttag atgacgcctg aacaagtgac
     8521 tgaagaattg ctaatagctg tgaaggataa tatttacgtt acctggaacg aagaagatga
     8581 gtcaattaaa aagatgatag ctaaaaatgc tgtttatctt caaagtaaag tgagtacaac
     8641 actttctttt tctcctgaaa gcttagaata cggattgcta atcgaaagat gtagatacga
     8701 ctggaatcgt gctttagatg agtttgaaca aaatttcgct agtgagttat taggtttcat
     8761 tcaacattat gcgctacaag aatatattgc aggtgatgtg aatggcgaat aatcgtagac
     8821 tcgaagaaac attcaacgat ggttggttaa agattttgac gcaaactacc aaaagaaatg
     8881 aactaggaaa aaagattggt gtagaagata cagaaatcac ttctttaaaa tttagaaatc
     8941 tttccatgag agatagtgat ataacagcta tggatgcgat gggatcgaaa ttaactaaga
     9001 aagtaaagac tccatttcat ccaatcgcca agaaatttaa taaagatcaa tattttatcg
     9061 taatcgatag tatgcgttac aacgttatct atgccgatta cgataatttt tatatctatt
     9121 tttatcttga aagtgtgggt gaatatggtg attgataatt ctaaagaaaa agaacgttta
     9181 aataagcaaa tttctgctat caaaacttcc ttagaagagc attttggcct caaactcttt
     9241 caagactccg taggcgagga tgagctacct gatgatttta attacttcat tctcgaaaca
     9301 ggagaaatag aaatgatcac tgagccaaaa tatagcgtgg gtcaaaatct atatctaact
     9361 ttctattcag aaaatagaga agatttaaca ggagactcac tagatattat ttcattgatt
     9421 caaaatcgtt cgattcgttt tcagagaatg gatcccaatc atttaaaact agagaaccaa
     9481 gatcgctata tcgatcaatt ggtatttacg tttagacgat tattgaagag tgattgtcat
     9541 ggctaaaaat agttgggagc taaaaataaa tggacatgat gaacttcttg tgcggatgga
     9601 acgctattca agcgagagcg aacgactgat taacgaagca ttgaaatcaa aaggttcggc
     9661 tattgcagtg gataggatta cagaaaaaat tcctgtttct gaagcagatt taagaagagg
     9721 gcaccaacac gcaaaaaata gtcgtccact taagactcaa tacattaatt tgggtttcat
     9781 cattagacct acaagaaaat ttgagtattt aaaatatcct gatttgggga taggtacttc
     9841 taaaagaaat cagccggacg aatttatgag aagaggatta ggtcttgcac ttgatccaat
     9901 tacagaactt ctgattcgtc aattcgataa attaaataaa tagggggaac aacaatggct
     9961 aaaacaaaaa ctgtagtaac aacgttcgat aacgtaagta tcaaacgaat tgcttttaat
    10021 tttaagaacg caggaaatgc aatcgcaacg aattgtaacg gacaattaga tggcgaaaca
    10081 gaaatgcaaa cggtggttaa aaaatgtgga gcgacagaag taaaatcaaa atctaaacca
    10141 atcaatatga cggtaacaat tactgcacat gtaccgatgg aagtttatcg acgtttcaat
    10201 gggttgaaac aagatggacg tattaaacca ggcatttact cttacggtcc tgattccgta
    10261 ggcgaagatt tctcacttgc tgcagagatc gtggatgact tcgaagaaaa tagaaagtta
    10321 gttggtatgt tagcatgcac ttcgaataca ggattaacat tctctattga aaatggtgcg
    10381 gatgaagtag ctgcgttaga actagaaaca aaagttatgc aagatgaatt tggtaaattt
    10441 tatcatgaag caattgttgc agaacttgaa gaagacttaa cagatcaatg gatgacgaat
    10501 ctatctgctg atgtgattaa aaaaacttca gttgtgacaa ctacggccac tcaatcacag
    10561 taaaaaaaac ggaggtagcg aaatgaacga agattactca aaaattgaac taaacgatgg
    10621 aacaattttg aatttagaac ctaaactgaa tatcaagaaa ttattgatga tcaatagaga
    10681 ttttaacaca gacgagtttg caaaaatgac tgtgggaaaa ggatccatgg atatttctgt
    10741 tattcaaggt gcaaaggctg tgtatattgc ttaccgccaa gcgaacatga ctgattatat
    10801 ttcattcgat gaatttatcg ataaatggga ttttgatatg gctactgcca gctatattta
    10861 tcaattgatg atgttcaaac aagcacgcga tgcttatcaa aaagaatttg aaaaagcaaa
    10921 taaggaaaaa aagcttcaaa agtaaaaatt ccaaagctct tagttgaaac gtgggtcgat
    10981 gtctattcga tgttgaccga cgttttttct atgccttcag atttggtttt aagcgatatc
    11041 tgtttagatg acattttgca aatggcttac aacaagagtg cttatgaagg atggaaaaac
    11101 tatgcaataa accaatccca gaaaaactaa agaaaggagg taaaaaatgg ctaaaaagag
    11161 aacagaagca gaagtaactt tcatagctaa cgatgacgga ttgaaatcta cgttaaaaga
    11221 aatcagtgct gaattaacta aaaatagagc agaattaaaa ctagaacaag ctcaattaca
    11281 acagactggt tctgaatcag acaagttagg aagtaaatta tcttctttag aaaagcagta
    11341 tgaattacaa agtcaaaaag ttgaagtaac tagccaacgt ttagccaatg cgaaaaaata
    11401 ttatggagaa aattccaccg aagttaagaa acttgaaaga gaactgatta atcaacaaac
    11461 agcgcaacaa cgtttgtcaa acgaaattga taaaacgagt aatgcactag ctcaagcaaa
    11521 aggcgaaata cagacgtacg agtctacaat gcaacagttg gatagcgaac aaaaaaatgt
    11581 tcaagctagt gcttctctga ttgaatccga atacaaaaaa tggcaagcaa ctgctggtca
    11641 atcagcttct gaagccgaga aattagcgaa agcccaagaa tatgtttctc aacaatctga
    11701 aaatgcggag aaaacgatag atatcctaag acgacagtta gaagctacac agtctgaatt
    11761 tggcgctaca tccacagaag caatgcagat ggaagcgaag cttaatgatg ctgaacgtga
    11821 atttgaagag ttaggacaag ctgctaaaaa tgtagataca actaacttgg acgatatcgg
    11881 aagcaaaata gatatgaata atttaatgga agcttctgac gttttaagcg acattggcga
    11941 taagcttaca gaattaggga aacaagcagt ggactctgct aacagtgtag gtagttccca
    12001 gagtaagata caagctaatt ttggtttgtc taaacaagag gctgaagaat taacgaatgt
    12061 agccagagac atttattata aaggttttgg agaatcgtta gatcagtcca cagatgcatt
    12121 gattttggta aagcgtaatt taggagattt aaataatcaa gatttacaaa atatcacgga
    12181 acaagctatg gtcctagaaa acaccatggg cgctgatatg gatgaaacgt tacgtggtgt
    12241 aaatggctta atggtcaatt tcggcttgag tgctcaagat gcaatggatt taatggtttc
    12301 gggtactcaa aacggtttag ataaaacgca cgaattaggc gacaatatgg cagaatatag
    12361 ccaattatgg agtcaaatgg gatattcagc tgatgaaacg ttcggaatgc ttcaaaatgg
    12421 tttagatgcg ggtgcttata accttgataa agtcaatgac ttagttaagg aaatgggaat
    12481 atcgttaaca gatggtcgat ttgagcaaaa catggatatg tttagtgaaa gtactagaaa
    12541 agcttttgaa gagtggaaaa atggcggagg aacacaaaaa gacgttatta attccatgat
    12601 tcaagatttt agcaatatgg atggtcaata cgaccaatta aataaagctt cgacaatttg
    12661 gtctgcgctt ggcgaagata atgcgatgaa agttgtccaa tctttaactg atgttaacca
    12721 tacatttgat gatgttagtg gatctgcaca aaaaatgaat gaagattcta ctactccgtt
    12781 gcaagagttg aacgggaaaa tagctgaatt aaaggattca ttagctccta taggcaacac
    12841 aatcatagat gcactcgaac cagtaattga ttttctagga aagatggctg atgcgtttaa
    12901 taatcttcca caaccagtac aggatttcat tgttgctata ggtggtctta cagcagcatt
    12961 tggcatactg ttaccggcaa tactagccgt gtcatattta tttggtccga tgatgcttat
    13021 aattggagga attatagctg ttatagcagg cgttattgtg gcaattaaga actggggtgc
    13081 aattactgac tggtttagtg gtttatggaa aaaatttact gattggttgg gtgatacttg
    13141 ggaaagtata aaagacggag cctcatcagt ttgggatggg gttaaagaaa cctggtctgg
    13201 atttgtagat tgggttcaag atatttggca aggagtttct gattggtttg gagagctatg
    13261 gagcggatta gttgaaggag cttccaacat ctggcaagga gtccaagaaa cttggcaaac
    13321 attcgttgat tgggtttcaa atatttggaa cggagtcaaa gaagtatggt cgattatttg
    13381 ggcagacatt gtaggaattg ttcaaatacc atggacatta ataacgtcat tgattcaagc
    13441 tggtattaat attatcgtgg gtatttttga tgtagctgga cagttattgg gcgcagcttg
    13501 gcaagctgtt tggacaccta tttctgattt ccttaaaaat acttgggata ctatgacaca
    13561 atggataagt atcgcttgga atgggattgt aactacattc catactatat ttgatccagt
    13621 agtggcatgg tggaatggta tatggacagc tattagtact acggcttcaa atatttggaa
    13681 ttcaattagt gcaacagctt ctagtatttg gaacagtatc aagaatacaa tcactagctt
    13741 ggtacaagca gctgctacag taattcaaaa tatttggtca actgtatcta gttggttagg
    13801 tggaatttgg aattcaatca gctctacagc atcaaatatc tggaatagtg tgactagtag
    13861 tataagcaat gctataaacg cagctaaaag tgccattcaa agtgtttgga atagtatatc
    13921 ttcgtggatt agcggaattt ggaacggtat caaaaatact gctttgaatc tttggaatgg
    13981 aattacaagc actattagct ctaaagtaaa cgatggaaaa aatgcaattt caagcggttg
    14041 gtccaatcta acaggtattg tttccgacat attcaataat gttaaaagta caattgctaa
    14101 catatgggaa ggcatcaaaa agactgttag cgctccaatt gattggatta gagacaaaat
    14161 cagtggcatt tttgataatt tgaatatttc tataccacat attccgttac cggaatttat
    14221 catggaaggc agctttaacc cgctaaaagg tcaaatcccc catttgcgtg taaaatggca
    14281 tgctaaagga ggtatcttta ctaaaccaac tttactaggt ggaatgaacg gtgtcggtga
    14341 agcaggacct gaagcagttt tacctttgaa aagatctgtt ttgcaagaaa ttggtgatcg
    14401 tatcttgagt agcacatcag tttcatctag ggcacaaacg attcaacctg tgaataacta
    14461 cgaattcaat ttcacaattg atggtaacgc agatgaggtt actatgaagc aaacaactca
    14521 acaaatcatt gatagcatta caaaaattca aaatgataat gcttcggcat ggcgttaaac
    14581 aggagagtat ttctcctgtt tttttagtat taaaaaggat gtgaaaaaat gactgattgt
    14641 atacattcta taatcgatgg atttcctgat tatttgcata aattggcttt agctgaaaga
    14701 ccaaccatac cttctccaaa aagacagaga gttgaaactt ctgttttagg taggttaggt
    14761 ggcttagtac aagattactc gtttgaagat atgtcgttta cattgcacta taactattta
    14821 gaggatgtgg aagaccatca agcgttcaag caatcgtttt atatcatgcg tcattggtta
    14881 aactatgcaa agaaattaga attctctgac gatcccaacg tctattacgt tatccagact
    14941 atcgatattg gggatgcaga aaacgatatt gttgaatggg gagagttcga tgtaaatatt
    15001 actgcgaaac cattcgcaag agttcaagaa gatgtaccta taaccgtaga taaaccacag
    15061 tcatttaact tgctgaataa tagtttagaa gaaagttttc caaagattat catcactcct
    15121 tcagctactt catgccagtt catcttaaat gattatgtgt ttagtttcga aggcttagta
    15181 gcaggaactg atgtagtcat tgatagtgat ttgatgcttt gctacgaaga gcaatcggac
    15241 ggagatattt tagatcggtc caacaaaatg aagaccatgc aatatccgac attgcaagtg
    15301 gatatcaatc attttaattg cactggtttg agcaaaatac aaatttatcg taacgggtta
    15361 aggtaggtga aatagatgat cgataattta ataactattt acgataaaaa tgacgcgaat
    15421 aatttagctg aacatttata tgatacgcaa ggtttaggcg ctttgtcaga ctggttaaca
    15481 gctactgtta gcaataaact aaacggagcc gagatatttc agggtactta ttcaataaac
    15541 ggaactaatg cagatttgat tgtagaagga cgtattattc agtgttatgt agatgaaaat
    15601 cgagcaaaac agcgtctacg gatttattat gcaaaaactt ctgtaatagg caatacgata
    15661 gaagtaaaag ctgaacctat tttcaatgat ataagaaaat cggtgttgaa taaatatgac
    15721 agcggaacag aaaagatcac tgctactcag gcatggcaaa acgcaaaagt tttagcgaaa
    15781 ccagctatcc cttcgcagtt ttctttctcg tcattagtag atacgcttgc taatgtgaag
    15841 atagaaaagg cgaatttttt agaattcttt ggtggaaaag agggatctat tctagatcga
    15901 tttcatggtg agtttctaaa agataataac acattacgtc atgaaaaaag tctaggtacg
    15961 gatcataaaa tcaaagcgat ttatactaaa aacttaactg gtcttgactt agagattgat
    16021 gctcaaagtg ttttagttgg agtttatcca ttcattagca gctcttcaga aggagaagac
    16081 gagatcactc taccagaaga agttattttc acggattacg tggatgatta tcctgctgga
    16141 tatgtttctt ttgttgattt taaagacaaa gcgactgatg tagccacatt aagggaagct
    16201 gctaaagact ggttgaaaac aaacatagat aaacaaaaac cacaagtgag tggttcgatt
    16261 gaattagtac cattgaggca tcaaagaggc tatgaaaaat ttgttgatct agaaaaagtt
    16321 tcgatgggtg acggagtaga tgtgtatcat ccacagttaa aagtgaatat gtcagcgaga
    16381 attgtggaat atacgtttaa tgttctaacc aattcatacg ataaattagt tgtaggaaac
    16441 gtcaaaacaa acttcttaga aaacacagag aataatgtca gcaatttgat taatgatgcc
    16501 attgatcaat tgaaaaacgg tggcgaaatc agtgatttac tcaatgatat tgtagatcat
    16561 caaactgata tgattactgg tcaaaatggt ggttatgttt tattagatcc taaagaagcg
    16621 cctagtcgta ttttgattat ggacacacca gataagaata ccgcaaggaa tgttttacaa
    16681 atcaacaatg ctggtattgg tttttctaaa actggcatta atggaacata tgaaacggca
    16741 tggacgttag atggcggatt caatgcctcg tttattacgg ctggtgaaat agtaggaatt
    16801 actattagag gtactacatt aattagtgat ggtgctgatt atagaacaag tattgctaat
    16861 ggcaaaatga cttggtactc aaaaaaagtt aacaaagata ttatggagct agaagcacgt
    16921 gattatgtaa gtgctgatgc cggtattgta tcatacacca tgaaaactgg tggtggtttc
    16981 atgattagaa atccacaggg taacttggtt tttagtacgt gggataatgg taataacaga
    17041 ccgtttctat cttttggtgc gcccaatttc aggtatagca atgctagtta tgtaacttct
    17101 ggcgacggta gttctttaag cattaatggt agtgcgggta actcatggga atttaaggta
    17161 gctggtagga ctatgaaatt tactagtgat ggtatgctaa cgttaccagg ttgttttttt
    17221 ggttcatggg aagatgggaa acttgctagg tttgaacaat caacggtaca agtatataaa
    17281 gattttactg ttagaggtac taaaaactca actgtaccaa cagaacatta tggacaacga
    17341 ctattgaacg cttatgaaac tccagaatat tatttcgctg attatgggga agccgttaca
    17401 ggtgacaatg gtaaagttcg tgttgatatt gaccccatgt ttgctgaaac agtaaatcta
    17461 agtcggtata tgacacatgt gacacctaca gaactagttt tgtgtgctgt tactcatgaa
    17521 gatattgacc atttcatcat tgaaactagt aagccaaacg tattagttag atggaattta
    17581 gtggcacacc gtctagggta tgaagatatt agattaaaag aggatacagc atatgatagc
    17641 acagtgcttg accaaaaacg tttttaaaac gaagacaagg aggtatataa atggctagca
    17701 gtttatataa tttggcttta gatttcagca aagaattaaa ctacaccaaa gctattatgg
    17761 ctcgtcaagg tgataaaggg attacggtga cggttaaacc gtttctaaat ggcttgcaga
    17821 tggatacgag tggcggaaca tttactttaa aaggaacaac accatctaac cgttacgtag
    17881 ataatgttgc aactagtgta actagtgaag aagtcacgtt ttctcttgat ggcacattta
    17941 tgagtgaagc aggatattat aaacactgct acgtagaata tagaaaagac aatcaaattt
    18001 taacaacgca agatatcatt tttttctcac taggagtgtc tgacatttcg caaggccaag
    18061 ccgatgaata tgtttcgcaa ttagaagagt tgattcgaaa gtacaacgaa acttttgatg
    18121 cttttatggc tgaaatcaaa ggtagagtgg atagcttaaa tcaacagatt actgatttaa
    18181 ctggtcaagc taaaacgcta caagacaagt tagatgctct gaaagaagaa atttctaagt
    18241 taggtaactt acaagtgatg tacagtaaca gcatcgactt cggggactat gattattcgg
    18301 agaacccgaa tttaatgcca tatattacag aaccttgggt tggacctttg ttaggtaatg
    18361 gacatacagt taaagatagt gttaaaaggg ttataactca tactaaaact agaacagcta
    18421 attccggcga catattatcg cttggcttag gtattccgtg tacagcggaa gctaataata
    18481 ggtatcttat aaccacttta cgaccatcta cgacatacac attaagcgtt actatgagtg
    18541 tcggttcaga ttggactgga gagaccaata ctataggggt taggcttaga tatttaaatg
    18601 aacaaggtgg tatagaactg cccattaatg cattaatacc agcaaatgta gaacgcgata
    18661 aaatggtcac tcatacattt actggtataa caaaagataa tgtaactagc ataacaaact
    18721 gttatgttga aatattctca cttaatagtg aatataaagg aacggttagt gtcagctatg
    18781 acgttaagtt aaaagcacac taccctaact tactagatgg cccttactgg ttaggtaaag
    18841 ttcctttagg cgagaatatt gctgacccta cagttgtctt tccacacaaa actagtgaat
    18901 atatggttta tggtagacgc aatacagaaa actatatcgc tgaccaaaca tataccatat
    18961 caatgaaagc aactaaacta acagttcaat cgtttgccgt gtatattgct gctggccgcg
    19021 ttaaagtagg agacatgaag cctactgagg ggttagctaa tacatgggag ttaactttta
    19081 ctgtaactaa gcaacatata gatagtgggg taactaatta tctggaaata taccaatacc
    19141 cgtcagctac aaaaggagct gttcaaattg agtggctcaa actagaaaaa ggcaatactc
    19201 gaaccccgaa tattagtgag tataaatatc gtggtactgg tatgcgtgat tcaaacaatc
    19261 caaaagatta tgtttgggat ctagcaccag aatatgtcga agataatttg gccacagata
    19321 ttaaaatttc tgaaattact ggtaaagcaa acaattatac cgatgggaaa gtatcggaga
    19381 ttaattcgca gttgactgct tcaattaatg aagtagacac cacagctaag gatgctcaaa
    19441 caaaagcgaa tgctaatgcg actgctatag atgaattaga caataagatc gatgaacgca
    19501 ttaatgatac agctactacc acattaacag ttacaaacgg gaataccgga tcagcaaagc
    19561 tttatcgtga aggaaaaaca gtttctatat attttgtggc tttaaacgga aaaagcagtg
    19621 gtggaaatga ttcaacgata ctaacaattc cagaaggcta tcggccacca attagttttg
    19681 agcaactggt tggctcgata gaccgttcta ctttgaacag tgctcagtta tctattggtg
    19741 cagatggagc cattaaatgg cgaagaaact caagttatgg atcggattat acctttgcaa
    19801 ttacttacac gatttagaaa agcgtgaatc gatatgaagg cagcatatag accaattgaa
    19861 ccttacggat tcgagcaaat cattgtgaat gatgaagaac atttaccgga agaatgcaca
    19921 gaagtcgaac caccgattcc aaattggaaa ccgaaattca attactggga gggaaataaa
    19981 tgaaaaacat ttggaaatat ggacgtactg gcggagagta cgcaggaaaa gtattggacg
    20041 acatgcttgt atccgttcct tacacagatc agccaccgct tgaaggaatt cgtgctgatg
    20101 gcgaaccgtt aacaatcgct gatcaaatgt ttgatcctaa attgaatcaa tggattattt
    20161 tagcgaacgc actagatcac aacgatttaa acaatctcaa agcaatgtat gagtcgttag
    20221 aaaatgagaa cggcgattta aaacagatca atgccaaact catgctaagc gatgtagcaa
    20281 ttaaacagga aaatactgca ttgaaagaaa aagcggatag tttagcacaa atcaattcaa
    20341 aaatgatgct tgcttcgtta caaaatagca aagacatttc agaaattaaa gagcaactaa
    20401 atccagcttc aaagggaggt gagtagtatg tttagtttta gcgatgtgaa aatgatgtat
    20461 gattggggct gtttcactaa cgaacaagta atggttttcg ttccgttgtg cattactgaa
    20521 gaa
//
LOCUS       DACEQH010000049        19309 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.049, whole genome shotgun sequence.
ACCESSION   DACEQH010000049 DACEQH010000000
VERSION     DACEQH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.049"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            968..2218
                     /locus_tag="IW833_001947"
     CDS             968..2218
                     /locus_tag="IW833_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ1308050.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            2237..3247
                     /locus_tag="IW833_001948"
     CDS             2237..3247
                     /locus_tag="IW833_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAQ1308051.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDVHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFEDGGTRVIHYTPETYIEKQ
                     NG"
     gene            3257..3910
                     /locus_tag="IW833_001949"
     CDS             3257..3910
                     /locus_tag="IW833_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1308052.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            3929..4927
                     /locus_tag="IW833_001950"
     CDS             3929..4927
                     /locus_tag="IW833_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ1308053.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWKPTVSLEEGLRESYNWIEKQIKG"
     gene            5003..5764
                     /locus_tag="IW833_001951"
     CDS             5003..5764
                     /locus_tag="IW833_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAQ1308054.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            5782..6888
                     /locus_tag="IW833_001952"
     CDS             5782..6888
                     /locus_tag="IW833_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1308055.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRDVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            6878..7111
                     /locus_tag="IW833_001953"
     CDS             6878..7111
                     /locus_tag="IW833_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308056.1"
                     /translation="MINDMEVTKLMALRNRYALNIVDNCTRKIAKILGCCIGKGAQIG
                     NSVEFVHNSVGTVIHSDTILEDGVKVYQNVTCG"
     gene            7310..7765
                     /locus_tag="IW833_001954"
     CDS             7310..7765
                     /locus_tag="IW833_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAQ1308057.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            7779..8552
                     /locus_tag="IW833_001955"
     CDS             7779..8552
                     /locus_tag="IW833_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1308058.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            8539..9669
                     /locus_tag="IW833_001956"
     CDS             8539..9669
                     /locus_tag="IW833_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1308059.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            9700..10935
                     /locus_tag="IW833_001957"
     CDS             9700..10935
                     /locus_tag="IW833_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308060.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFVPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLSVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            10960..12069
                     /locus_tag="IW833_001958"
     CDS             10960..12069
                     /locus_tag="IW833_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308061.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAKYEKYMDFVWEE"
     gene            12111..13445
                     /gene="gmhB"
                     /locus_tag="IW833_001959"
     CDS             12111..13445
                     /gene="gmhB"
                     /locus_tag="IW833_001959"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAQ1308062.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            13480..15117
                     /locus_tag="IW833_001960"
     CDS             13480..15117
                     /locus_tag="IW833_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ1308063.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            15219..15614
                     /locus_tag="IW833_001961"
     CDS             15219..15614
                     /locus_tag="IW833_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ1308064.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            15632..16363
                     /locus_tag="IW833_001962"
     CDS             15632..16363
                     /locus_tag="IW833_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308065.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            16387..17838
                     /locus_tag="IW833_001963"
     CDS             16387..17838
                     /locus_tag="IW833_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1308066.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISIINK
                     FDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            17864..18475
                     /locus_tag="IW833_001964"
     CDS             17864..18475
                     /locus_tag="IW833_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1308067.1"
                     /translation="MIDIISAIMVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            18657..19172
                     /locus_tag="IW833_001965"
     CDS             18657..19172
                     /locus_tag="IW833_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-keto-6-deoxy-D-glucose epimerase"
                     /protein_id="HAQ1308068.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNGERLSF"
BASE COUNT         6223 a         2699 c         4218 g         6169 t
ORIGIN      
        1 ttaccaacat gtttagacat tttcctctag gaaaaaatta actagcacca cgggtgatat
       61 atatgtatat ggtgactgaa gacaaaaaaa cagaactaat gaaactgaat aaaaatatag
      121 agtgaactag tttctaagag ggattttaaa ctagatttca ttggtaataa aatttggaga
      181 gatggaaaaa cagcacttaa ggaagcagat tttgttgtca tttttgttcc tacaaactat
      241 ggtagtgaat gtgttattga attagtgatg tctgttattt cagaggcatt ggggataatt
      301 aaatccacta tacctgtagg atatacaaaa agtggaagaa aaaaatatta tcaatagatt
      361 ttatttagtt cagaattttt acgtgaaagt agattattaa tgataatctt tatccaatta
      421 gaattattat cgtaggatgt gatgatgaaa gccgtgaaat tgttcaagta tttaccaatg
      481 tctccctgcc agtaaatccg cttagaattt gagccttgaa aatgtgcaat gataaaaaac
      541 aaaaactcgc tcaaaataga cagtttgggg tgggaataga ccaataatgt accagacaaa
      601 cttccatttg gggtgaaaat aggtcaataa agcgatgtta aacatacctt tggagtaaga
      661 atagagggta ataagtgaat ttataaactt tttggtgact ggttgagtgg aaaatgtagg
      721 tacatttcaa tgcgaaaatg tattataacc ccaaacgagg tcaataaaaa tgactcaaac
      781 ttgcaatata accctaaacg ggtaggggat ttatgaaaaa ttgaatttgc cggcgtagga
      841 gctggctgtt ttaagtatga atgaacacag ctttcgggct gttttatata aatagcaggt
      901 tactgttcac aaacagcatt ttgttaagaa agtagtgacc acagcagtgg tggaaagagg
      961 aaatattatg aagaaacagt ataacattgc agtcgccggc accggttatg ttggtttgag
     1021 tattgccact ctgttggcac agcaccataa ggttacagcg gtggatatca ttccagagaa
     1081 ggttgaactg attaataacc gtaaatctcc aatccaggat gattatattg aaaagtacct
     1141 ggctgagaaa gaactgaatc tgaccgcaac attggatgca aaggcagcat accagtctgc
     1201 ggattttgtt gttattgcgg ctcctactaa ctacaatagc cgcactcaac attttgacac
     1261 atccgctgtt gaggctgtta ttaggctggt catggaagta aatcctgatg ccattatggt
     1321 tattaagtcc accattcctg ttggatacac cgcttctgtt cgtgtaaaga ctggtagcaa
     1381 gaatattatt ttcagccctg agttcctgcg tgaaagtaag gcactgtatg acaatctgta
     1441 tccttcgcgt attattgtgg gtaccgatat ggatgatgag cgtttggttg aggatgcaca
     1501 cacttttgct acactgctac aggaaggcgc aatcaaagag aatgtggaca cattgttcat
     1561 gggctttact gaagcagaag ctgttaagct gtttgccaat acatatctgg ctcttcgtgt
     1621 cagctacttc aatgagctgg acacttatgc agagatgaaa ggcttgaaca ctcagcagat
     1681 cattaatgga gtttgccttg atcctcgtat tggtactcat tacaacaacc cttcctttgg
     1741 ttacggtggc tattgcctgc cgaaggatac caagcagctt ctggccaact acgcagatgt
     1801 tcctgaaaat ctgatcgagg caatcgttga atctaaccgt actcgtaagg actttattgc
     1861 ggatcgtgta cttcagatgg ctggttatta caattacgca gaagcaaatc agtatgatgc
     1921 tggcaaagaa aagcctgttg tgattggtgt ttaccgtttg accatgaaat ccaattctga
     1981 taacttccgc cagagttcta tccagggcgt tatgaagcgt gtaaaagcta aaggtgcaac
     2041 tgttgtgatt tatgagccga ccttgaaaga tggagagact ttctttggca gtaaggttgt
     2101 gaatgatttg gaacagttta aggcaatgtg tgattctgtt atagcaaatc gctacgatgc
     2161 ttgccttgat gacattaagg aaaaagtata tacccgtgat atcttccaga gagactaaga
     2221 ttaggagaga gtatttatga ttattacaaa aacaccgttt cgtatgtctt tttttggcgg
     2281 tggaacggat atggaggatt atttcaaaga gaatggcggt gcagttcttt ctactacttt
     2341 tgataagtac tgttatgtga atgtgagaca tctgcctaga ttttttgatt acagcgctga
     2401 gttatcttat tctcgtactg agagagtaac tagcattgaa gatattgagc atcctgcgat
     2461 tcgtaatgcg atgaaaatgc tcgatgtgca tgaaattcgg cttacatacg aagctgattt
     2521 acctgctcgc tctggtctgg gtacatccag ttcttttgca gttggtatgt tgaatgcttt
     2581 ttatgcactg gagggtaagt atgctgacaa gaagaaactt gctgatgaag ccatttatct
     2641 tgaaagagtt ctgtgtaatg aagccggtgg atggcaggat cagattgccg catcttatgg
     2701 tgggttcaat cgaattaatt tcaatgtgga tggttatgag gtgctgcctg tcattatttc
     2761 gcctgaacgt aaaaagcagc ttaacaacaa tctgatgatg ttctttaccg gattcactag
     2821 attttcttct gatgttcaaa aggctaacaa cgtcagtggc acagaggata agagagtaag
     2881 actgaaaaag atgtatgagc ttgtagatga agctgaggca attcttactg ataagaacag
     2941 aaatttagat gatttcggta gacagcttga tgttacttgg agattgaaaa aaggtactgg
     3001 tggagcaatt agtacaggaa gcattgatga actatatgaa aagggtatgg ctgcaggtgc
     3061 tttaggcgga aagttgctcg gtgcaggtgg tggaggtttc ttggttttct atgtacaacc
     3121 tgagaaacag gatgcagttc gttgggctat gcgtgatttg atgtatattc cgtttgaatt
     3181 tgaagatggt ggaactagag ttattcacta cacgccggaa acttatattg aaaaacagaa
     3241 tggataagga gtaacaatgt tagcggaaaa acttaataaa catattgact tattgattaa
     3301 acgatatcct acacttgaga cgattaaaca ggacattatc gatggatacc taattatgga
     3361 ggaatgctat ttgaatggtg gtaaacttct tgtggcagga aacggaggat cagccgctga
     3421 ctcggaacat attgctggcg agttgatgaa gcggtttaag acgccacgtc ctgtaagtaa
     3481 ggaatttgcc gataagttag ttgcaattga ccctgtaaga ggtccggaat tagccaaaaa
     3541 tctcgagtgt ggtttaacgg caattccttt ggtcgcacac gaagcattgt ctactgctta
     3601 tattaatgat gtcgatggtt tgggtgtctt cgcacagcag ctgtttggct ttggtagatc
     3661 tggtgacgta ttccttggta tttcgacgtc cggaaatagc aagaatatta tgagcgcaac
     3721 tgttgttgct agggctctcg gtataaaagt tattggcttg acgggcgcaa agggtgggga
     3781 acttgccacg gtagcagatg tttgtgttaa ggctcctgaa gttgaaacat acatgattca
     3841 agagtatcac ttgccgatat atcattgttg gtgtttgatg ctggaagata aattctttgg
     3901 aaacgaataa aatttggagg aatgttaaat gaaaaagaca attatggtta ccggtggttg
     3961 cggtatgatt ggctccaatc ttgttaagag actggtgaag gaaggctgct gggatgttta
     4021 tgtagctgat aatctgtgga gaggaaagtt ggagtacctg aacgacgaag atgggcatcc
     4081 tgtcatcgac ctggatactc atttcttcaa tgctgatctg actgattatg agcaggctaa
     4141 aaaggttatt ggtacgactg agtatgtagt tcatctggct gatgttgtgg ctggcattga
     4201 ctatgtgttt aagaaccagg gcgaattgtt ccgaatcaat aatctgatca attctaatgt
     4261 ttttgattgt tgccgtaagg ctggcaagga gaaaatcaag ggtgttatct atgttggtac
     4321 cgtatgctcc ttccctctga ccagacagaa tactttgaat cctgagcctc tccgggaaga
     4381 ggagttgttc cctgctcttc ctgagtctgc ttatggctgg agcaaactga tgggccagtt
     4441 ggagatgggg tatttggaaa aagaaaccgg aattccgtgc tgtatcctga tgcttcataa
     4501 tgtttatggc acacctactg attttggcga acgtagtcag gttattcctg ctctgattcg
     4561 taaagcaatc aataatcctg aagagccttt taatgtgtgg ggtagcggag agcagggacg
     4621 tgctttcatt catgtcaatg atatcgtaaa tgcattggtg ctcgccctgg ataagggttg
     4681 gggtcatggt catattcaga ttggcccttc tcactgtact tccatcaagg aaattgcata
     4741 taagatcatt gaaatttctg gcaaagacat caagcctttc tttgatactt ctaagcctga
     4801 aggtgataag gctcgttgcg cagattattc taaggcgaaa gagattttgg attggaagcc
     4861 tactgtttct ttggaagagg gtcttcgtga atcctacaat tggattgaaa agcagattaa
     4921 gggttaataa gtatataagc tgttcttcct ccgtgtaatt agaggatgcg gaggaagaac
     4981 atatgagtaa ggatgtactg agatggaaaa gaatatgtat aaaagaaaac gtgatttcac
     5041 tgtgtgtgga atagtaggtg tttgtgctac tacaaagaac gagtttgtaa gggaagtttt
     5101 ttccatgcca acatctccgg taagtgcgac tgttgatctc gtgggagtgc cggccattat
     5161 ttcggcaaag gaaaatccca aaattgctga gatgtatagc caggctacaa tggctgcaat
     5221 agatggtatg cctattgtta aaattggacg gcgcaaagga tttacctgtg aacgatgtgc
     5281 tgcaccagat attatgggaa tggtttttga agaaggcgtt agacagggaa aaacacatta
     5341 tttctatggt ggaaaagata acgaagtgtt aaataagctt cgtgcaaacc tagaaagaga
     5401 ttacccggga atcaaaattg ttggaatgta ttccccacca tttcgtccgt taaatgaaga
     5461 agaggatagc aagatttgcg aggaaatcaa caacttaaat ccagattttc tgtgggttgg
     5521 tattggtgcg cctaagcaag aaatgtggat gtgggaacac cagaagaaga tccataacac
     5581 agtaatgttc ggtgtaggcg ccggctttaa cttttttgcg ggcactttgg aaaaagctcc
     5641 tgaatggatg gaaaaagcta gtctagagtg gttatttaga ctggtaaagg aacctcgtag
     5701 actatggagg cggtatatct tgggaggtat aaagtatatc tactatacta ttgaatctaa
     5761 gtaggggggc aataaaaata gatgaaaata cttatagtag ttacaatgac gaataacttt
     5821 ggtaagaagg ggtcctataa ttctcaggaa ataggattag ctcgagggtt agctgaatta
     5881 ggccacgaag ttattgtata taaaagtgta aaaagtgatg aggaagttgg aacagagcaa
     5941 ttaagcaata cggcgatgat tcactatatc aagtgtcgtt atgtaggatc aaatggattc
     6001 ttatcaacaa agaattttga taagaatgcg gatgttctaa tacaatttgc ggaccttcaa
     6061 ctatcagtac ctcatgtata ctcatggact gtaaaaaatg gaataaaata tataccttat
     6121 aatgggataa cgcgaagtca aagtggcaat aaatatataa agacaattat tgaaatattg
     6181 gcgcatcgaa attataaaat ttataagaaa aatgggtgtg ttgccaaaaa tgatcgagtt
     6241 tctgaaaaat tagtacaatt aggagtaaat aaagtgaaac ttgctcctgt ttgtgtggat
     6301 tttgaacagt tacataagaa ttacgaagca atagatttta ctactctcag agaaaaatgg
     6361 aatattcctc gcgatgttcg tgttatttta tatgtaggac ggtttgaaaa ggataaaaga
     6421 ccgttagata tgattagttt atttaataaa cttgatgagc ataattattt gctgatggta
     6481 ggaactggtg ctttactcga gcaaacgaag cagcagataa atttacttgg actgcagaat
     6541 agagtaaaaa taattgagcg tattcctaac aaagatattt gggagttata ttgcttatcg
     6601 gatgtatttg taaatctaaa ctatactgaa atttggggta tggttttgct agaggcaatg
     6661 tactatcgcc tcggaattat tgctgttaat gcacctggcc cacagaatat tattttggat
     6721 aatagatatg gaatgttggc taaatctgac gatgaaattt tgacattctt aaaagacgga
     6781 cgatttacca acgatatcat agatgcagca tatcagcgtg taactacaga atttagatgg
     6841 tcaaagtcgg gagaaattat gcttagcatg ttggaaggtg ataaatgata tggaagtaac
     6901 gaaattaatg gcattgagga atcgatatgc tttaaacata gttgataatt gcacgagaaa
     6961 aatagccaaa attcttggtt gttgtattgg taaaggtgca cagataggga actctgttga
     7021 atttgttcat aattctgtag gcactgtgat tcattctgac acaatccttg aagatggtgt
     7081 taaagtttat cagaatgtga cgtgtggttg agcagatagt aatgaacgta tcatcattcg
     7141 agtaaaaaag aacgctgttt tatgtgtggg tgctaaaatt ctttgtaaat ctaacacaga
     7201 attatgcgtt ggggaaggtg ctgtagttgc tgcaaataca gtggtattga ggtctattcc
     7261 accctacgaa gtgtgggggg tacctgctaa aaagatgggg agataaaaca tgaatgcata
     7321 taaattatac aaagtagaac gatggtttta cttacacaaa atggagtttt ttgccaagat
     7381 tgtaaaggca ctaatttatg tacttcataa tagctcaatt tcatatcaaa cagaaattgg
     7441 agataattgt aagttcttat acggtggcat aggttgtgta attggaaaag atacaaaaat
     7501 aggtaatcac gttataatcg gaacgaatgt actgactggt ggtagatcca ataagcctgg
     7561 tatgcctaca attggaaaca atgtatatat cgctactggc gcaaaaattt taggtaatat
     7621 cacaataggg gacaatgtaa tagtaggggc taatgctgtg gtcattaatg atgttgaacc
     7681 taattgttcg gtaggaggcg ttccggctag agtattgaaa aaagatattg atgtaaatga
     7741 ttattgtagt ttgaatcgtt actaaaagga gagattgtat gttgtatttt gttattcctt
     7801 taatgagtgc gaaggcagca aacaattggg agatggttag taccttattt aaccgaacat
     7861 tatggtcgtg ttataatcag actgatcctg atttcaaaat cattgttgct tgccatgaaa
     7921 ttcctaaact cacgcatgaa tatgatgaca gagttgaatt tattcaagtt agcgaaaagg
     7981 atgcccctat tcccacaaat caagacgaaa aaatgattga taagggttat aaaacacatg
     8041 ctctcgcaat gcgtttgcga gagttgggcg gcggtttcgc tatgatggtt gacgcagatg
     8101 atcttgtaag ttctcatttg gcggaatatg tgaattccca tccccaggaa aatggatttt
     8161 atgtcaaaac gggatacgtg tactttgttg gagacgatta tatgaaggtt ctaccaaagt
     8221 tctcatcagg aagtgcgtgt atagtaaaat atgcagtgga agacctgcct gaggcatatc
     8281 cggaagttat gacagcaaat tgtgatgata atccatggat cattagaaaa cgacatgggg
     8341 gcgttgttcc agcatgtgaa gagcaagggc gtccattaaa acctcttcca ttcaaagcag
     8401 cagtatatgt attaggaaca ggggacaatc actcgcttta tggtaaaacg acaaaatatc
     8461 agacgaagat gcgagagata ctggaaatgt ttatagcaaa acgtaaaatt aaaggaaaac
     8521 tgaaagcaga attttctgtt gattggttat aaggtgagta ttatgaaggt attgatgtgt
     8581 gaaagccaaa tgccagagaa ttggtattcg tttgatcttt ctaaagaact taatcaatat
     8641 gttgatttaa cattgctgtg taagcgtgat gttcctcctt tagatcctaa aattaagagc
     8701 aaacatattt tgtatcataa aagtacaaat aaagtgtttt cggcacttgt ttttctgaaa
     8761 agcttgttct taatttggaa ggaaataaga tttggtgatt atgatattgt tcatttgcaa
     8821 ttgatgaaaa gtctcaaata tgaaggcgga ttgtacgttt tatgtaagag acactataaa
     8881 aaattaatta tgacagttca taatgtgtta ccacatgagg ttcgtgaaaa ggatcgccaa
     8941 gagcacttta atatatattc aaattgtgat ggtttcattg ttcacaatcg agtaacagaa
     9001 cagcatttat atgctgaatt cccagaatta aatgcaaagt catatgtttg tccacatgct
     9061 atctatggag atgttaaaca tatatctaga gcgtatgata aagataagtt tgaatttatc
     9121 ttgtttggac aaattcgtcc atataaggga attgatgtac tgcttaatgc aatcacagaa
     9181 cttccaatgg atgtaagaaa gaaagttcat tttaccatag caggtcaaca acatcctaaa
     9241 caggatagta ctgactataa aaaaatgatt gaagatttaa aggtttcaga ttgtgttcta
     9301 tataagccag agagaatttc tgatgaagaa atggacagta tgttcagaaa tgcagatgcc
     9361 gcaatatttc catataaaga aatctatgga agtggagcgc ttcttatggc atacacttat
     9421 tgcttaccaa ctattgttag taatgtacct gcttttatgg aagaaaccga ggaaggaaag
     9481 atagggttac ttttcgaaaa tttgaattct aagagcctag cagacacaat tgtaaagtat
     9541 gtgaacctta gcccagctga gaaaagcaaa atagaggatg atatatatga gcttgtaaac
     9601 cgcaagtata cctgggcggc atctgcaaaa ctcacatacg aggcatacaa acaaattttg
     9661 aatgagtagt aaaacaatta gaaaggaata ataattaaca tgcagattag taaatggaaa
     9721 atcatacagt ttctaatggt ctttctaatg attatctcat caagcatatt gaagcctttg
     9781 ggcggctggt tgggagaaac gatgaacgtt agacttgtga cagtcattct agcaatagta
     9841 tgtttgttaa tagcctcatt accagcattt agaaaaaata gatatttaaa tttctgcact
     9901 ttactgattt gtgttgttat aatagcagct tacatctact cagataagac aatatgggtt
     9961 aaatctaggg aaagtatgag tttatactat gtattattgg cgtatccatt gttctgtact
    10021 ttaattaacc aagcgtggaa tttggattca atgctaaata cgatatgtgg tgtaacattt
    10081 ctttcataca ttcttagaac atctatttca cttattcaga aattttcagg agtacttctc
    10141 tatgaaaaca tatatcgtga gtgtgccccg gaaaattggt atcgtggagg ctttttaaga
    10201 attaatccac catgttttac aattataata ataccgattg ctttgtatat gattgagcga
    10261 caagttgtag ctagaagaaa agtaaaatat tatttactga ttgctagcgc actggcatat
    10321 tctgctgtta ttcatggttc tagatctgtc cttgtctatc aaattattgt acttggttgc
    10381 tatatccttt ttaagaaagg ttcttcgaaa agaaaaattg caatgtgggt tttagctggg
    10441 ttgcttgtag tcatatttat taattcgacc atgttctcaa catttgttga aactttcaca
    10501 aacagtacag gtgaatatgc aggatctgct gaatctcgat ttgcttcagt ttggtttttt
    10561 gttcctaaat ttttgcaatc gcctttgttc ggtactggta ttcttactgg tgaggaattt
    10621 acgtttatat taccaggagg agttagagga cacctatctg atattggatt tttctataca
    10681 attactcaat atggtgtagg tatacttata tttgatattc tgtttattat aaaagggttc
    10741 aaaacagcat tacttgcatc aaaagtgggt atgactggct atcaatattt agcatttgga
    10801 attacattaa gtgttttgct aacagggatt aatatagact ggttttatcc gatatttact
    10861 ccagctattc ctgtatgctt agcggttata gaatacatct atcaagaatg tagaagtgtg
    10921 gctaacatag aataggaaat attaatagga gtgcatctca tgaataagaa agagatttta
    10981 tataacatat tgaagaagta cgaaaattta ccatatgtgt ttaggaacat gtttggtcat
    11041 aatataagta aactcgtatc ttggaaattg actgctgatt gtgcaacagt agaagaaaat
    11101 tctgaagttt atgcagcgtt tcaattgcgc tcaaaggaca tgtctgatgt tccagtgatg
    11161 ggtttagtta attgttgcaa agatgtagca attgtaatgc aggggccact atgtttaaag
    11221 gatgatttct cttataatac tctggcctac tataagaaat gttatccaaa tgctttaatt
    11281 attctttcga catggaagga cgaagatatt aaggcattga aaaggataga ggatcttgga
    11341 gttatagtta tttgtagtga gaaacctgaa aagcctggac acttaaatat taattatcaa
    11401 gttggaaaca ctttgcctgg aatagtcaga gcaaagcagc ttggtgcaaa gtatgtttgt
    11461 aaaactagaa cagatcaaag aatataccat ccaaatgcaa tggcgttttt ctgcagttta
    11521 cttgaacagt atcctgttaa taacgaagat gattggaaat taatacaaaa tcagcgatta
    11581 ctgttactat caatgccgta tggagacatg tttttcccgt attgtttgtc agacttcctg
    11641 tattttggtg atgttgatga tatgataaac ctcttctcga ttcctagtga tgtgagagaa
    11701 aagggagctg tgtctagagg agtctcaaga agggagatat ctgaaaataa tttagctcca
    11761 gaagttcagc ttcttcgaag ctatatttca cgaatggggg gcaatgagga gtgctctatt
    11821 agggcatatt gggagtttgt aaaaaaccat gttattacta ttaacaaaaa tcaaattgat
    11881 ttgtactggc ataaatatgt aggtcgttat tcaaataata ctatttatgg tacgtattat
    11941 attgatgata gccaagatgc acttcactgc tataattttg attttataaa ttggttgaat
    12001 ttgtacactg gaaaatacga atacaaagca aaatatgaaa aatatatgga ctttgtttgg
    12061 gaagagtaat taacttttat agtaaaaagt actttaggga agggcatatt atgaaaacag
    12121 taattatggc gggcggacga ggaagccgaa tttcggaatt gtttccggat attcctaagc
    12181 caatgattcc tatagatggc attccagttt tagaacgaga attgtgttcg cttagagatc
    12241 aaggctttaa tgatattatt ttgactgttt cctatttgca tgaaaaaata gaggagcatt
    12301 tcggagatgg ttcaaaatta ggaattaaaa tcaaatattt tattgaaaaa actccacttg
    12361 gcaatgcagg tgcattgtat tttttgttgg atgaactggg tgatgaaccg tttttacttt
    12421 tgaatgctga tgcagtattt gatgttgatt tcaggcagat gattagattt catgttgaga
    12481 ataacgcaga tgttactttg tttacgcatc ctaactcaca tccgtatgat agtggattaa
    12541 ttatatccga tgacaataag aaagtgcttc aatggttagc gaaggaagat caaagacctg
    12601 aatattataa aaaccgagta aatgcaggtc ttcatgttat aaatccgaag gtgttgagag
    12661 aaaatccatt aatggaaatt gtaaataaat caatagttga aggtgggggg ctaccataca
    12721 aaattgattt agatagaatg attctgaaac cattggctaa cacagggcgt atgtattgct
    12781 acgattctcc agagtatgtt aaagatatgg gaacaccgga ccgatataga gctgtaatta
    12841 aggactttca aaagggaatt attgttggta aaaatctatc caatccacaa aaagcaatct
    12901 ttttagatag agatggaacg attaataaat acgttggttt tttgagaaat gcagaagaat
    12961 ttgagttaat tgatggagta tcggaggcaa ttaagattat taattctcta ggttttctgt
    13021 gtattgttat aacaaatcag cctgtaattg caagaggtga agttactgtt gatgagctta
    13081 atagtattca caataagctt gaaacaatgc tagggcatgc tggtggattt ttaaacggta
    13141 tttattactg tccgcaccat ccccataaag gatatgcagg agaggttata gaattaaaaa
    13201 ttgattgcaa ttgtcgcaag ccaaaacctg gactgttatt agaagcatca aaggatttta
    13261 acataaacct aagtcaatca tttatggttg gagatagtga aagtgatgta attgctggaa
    13321 aaaatgctgg ttgtaagaca gtactgatta cagaagacat caccttgcct gctaattatg
    13381 gccaagacta tagtgtctgt tcattgttag aatttgttaa aaatcattta tgcactatgc
    13441 aataattggt ttagtgaaac ttgtaaaagg agtaattaga tgaaggtatt aattccagca
    13501 gcaaaagttg ttgaaaaaga attgcagagt ataggaatgt taccagcaat tatctatcca
    13561 attaataaaa aaattgtctt tgattatttg tataatcagt ataatgcaat ctgcgattca
    13621 ttggatatta tctgttatga aaaagcggat aaggttcata gaagactgaa aaactatcat
    13681 gattccagaa ttcatatatg tgaccttcct gaattagggg atctagggca aaccgtgtat
    13741 tttgggttat cagatgaaga tgagcctgtt ctaattaact tcgcagatac aattgttatg
    13801 gagagtatgt ctgatataga aatggataca ttctattatt ctgaagattt tgtgtcgagt
    13861 acttggacat actttaaaat taatgaagga ataattactg atatccacga taaagaggaa
    13921 gcttacgtat ccgataaacg tgaaaaatta tttattggcg tatttatgtt ttcttcgtca
    13981 aaacttctga agagttgctt aagagaggca gttgagagtc ggaaacaggg gggaataaac
    14041 tcattttatc gggctttaca gctatacagc gaacgttgtc cgctaaaggc cgtaagaaca
    14101 gaaaagtggt ttgatattgg acacatcgat aaatattaca atacaaagtt ggaagttaaa
    14161 gcacgagagt ttaatcatat ctctatagat aaagatcgag ggatattgaa gaaaactagc
    14221 gatgacaagg ataaatttat tggagaaata aagtggtatt taaaacttcc tgctgatatt
    14281 gaatatgtaa gacctagaat ttttgattat tctacatctt acgtagctcc ctatgttgat
    14341 atggaatatt atgcatatca tacggttcat gagttatact tatatggtga tttaaaatat
    14401 aaccagtggc atgatctgtt tagccgtata aagtttatct gtagcgattt taagcgatac
    14461 accttaaaag atgaaaagat tagagagtca ttggaagata tgtatttgcg aaaaaccatg
    14521 cagagatttc aaaaattgtc tgaagataaa agattttcag ctttttttga gaaacaaatt
    14581 caaattaatg gtcgtaagtt tatttcttta accggtgtaa ttgaacttct aaagaatctt
    14641 atcccaagta tgctctatga tgtaaatgaa ttttgtatta tacatggaga tttgtgtttt
    14701 gctaatatga tggtagattc caattgttcc tttgttaagg ttattgatcc taggggaaaa
    14761 tttggaaaat atgatattta tggggacagc agatatgaat tagcaaaact atttcatagt
    14821 ttagatggta aatatgatta tatcatcaag gatctattta ctgtagaata tgataccgag
    14881 tgtgcagtga tagattatac aattaatacg cgtgtaagag attacgattt atttgaattg
    14941 tttaagtcgg tcttttcgga cgagattaag gacgattttg ataagattgt attgatagaa
    15001 gcgttgctat ttcttagtat gattccatta cacggtgaaa gttttgatca ccaaatagtg
    15061 atgcttgcta caggtatgga acttcttgca agagtaagta ataaagttat actctaatat
    15121 aaattgtgcc ttaatgaata acggttacaa tactgtttta gcaatgtagg cattaaggca
    15181 cgattatatt aattaataca atagaaggga agagaaaaat gtatagtgaa tattcttttg
    15241 tgtttgatat cgatggcact ttgtgtccga taaagaagaa ggatgagcga tatgaggatt
    15301 tggtgccgta tgagaatatg gttgaaaaac tgagattcta taaggagaat ggtgctaaaa
    15361 ttacattgtt cacctcacgc aatgttaatt cctataaagg aaatttggga atgataaatg
    15421 ctaatactgc aaaagtgctc ttggcgtggt tggataaatg ggacattccg tatgatgaaa
    15481 tcctgtatgg taagccttgg ccggggcata aaggcttcta tgtagatgat cgaacggtta
    15541 gacctgatga gtttttgaga tgctctgtag atgaattaaa tgaaatctgc aacagttcaa
    15601 aaaaaagatt ctaaataagg aggttagttc tatggaaaaa aagatgacag ttatcattac
    15661 gatggcaggt ttgggaagtc gttttcgaaa aattggttat gcggttccta aatatatgat
    15721 taatgccaaa ggaagaactt tatttgattg ggccatggat agtttggttg gatacaataa
    15781 aaatgttgat aagtatattt ttgttgttcg tcaggaagac aacgcttcag cttttataaa
    15841 ggagcattgt gcttactttg gtattgaaaa cgtagaagtg attgaaatag actatatgac
    15901 agatggtcaa gcaactacat gtatgttggc tattccttat tgcaatcaag acaatgagat
    15961 tatggtttac aatattgata catacgtgga atcaaatgag atgaaatacg aagatatttg
    16021 tggagatgga catattccat gctttcatgc tgatggcgat cattggagtt ttgcaaagct
    16081 taacgatcga aatgaagtaa tagaagtccg cgaaaaagtt cgcatttctg ataattgtac
    16141 tcttggtgcg tattatttct catcggcaaa gctgtataaa gctttatatg aagagtatta
    16201 tgcagactcc tctaatatgg agaagaatga aaaatatata gcaccattat ataactttat
    16261 gataaaaaaa gggttaaagg tgactattag catagtagat gcgaaaaagg ttcatgtgct
    16321 tggtactcca gaagagctca atgtgttttt gaatcaagaa taaatagtgt ttaggagaaa
    16381 atagcagtga ccagtaataa tctaattgta aaaacattaa gcagatataa gaatactcca
    16441 cttcctgtta aggctgcatt atggtttatg gtatgtggtt ttttacagag aggggtttca
    16501 atgattacaa ccccaatttt tactagagtt ttgtctacag aacaatacgg tttatacagc
    16561 acttatctat cgtgggaatc cttattgctc atgattacat cgcttagttt gttcaaagcc
    16621 atgatgaatc tttatgtaaa atatgataat caagaacata ttctttcgac agtatccgga
    16681 ttgactttag tactgactat tttttggttt gttattgcta tttttattaa ggatatattg
    16741 gcagaaattt taggattgac accagttcta gttgtctgta tgttctcata ttttatattt
    16801 caggcagaaa tacaatgttg gtctttacac aaacgatata tatatgatta taagacttta
    16861 ttagtagcta cccttgcagt tactgttggc tcatctgtag ttggctgttt agcggttctg
    16921 tttatatctc ctaccgctga ggcgagagcc ttaatgaatg tcctagtgac tggtgttgtt
    16981 ggcgtggctg tgtatatatc ggtatttaga agatgtaagg attttaagga taatgaagtg
    17041 tggaatttcg cagtagtatt ttgtgtatcg ttgatgccac actatttatc tgaatttatt
    17101 ctgaatggtt ctgataaaat tatgataaat tacttatgtg gttcgtcaga tgttgctatt
    17161 tacagcgtgg catattctat tggtgctcta attaaccttg ttacaaattc tgtcaattcc
    17221 acttttgcac cttatcagta tcaaaaaatc aagtcaggag aatatgattt actatcaaaa
    17281 agagctaatg aagttcttgg tttggtagcg gctatgcttt ttgcattaat gctatttagt
    17341 aaagaaattg tgttgatatt tggtgggcag aaatatatcg acagtgttaa tttaattgta
    17401 cctatatgta tcggtattta tttcaactat atgtttcagc tgtttgctag agtacaggaa
    17461 tactttgaaa agaaactcac tgtcgttatt ccatcggttt tgtgtgcaat tatgaatatt
    17521 gtactaaatt atgtttttat aaagttatat gggtatcagg cggtagcata cacaactgta
    17581 ttctcatata tgtttttctg cgtcatccat tatatttttt atagaaaagt ttgcaaagaa
    17641 ctattggatg gaaaaaacat atatgatgtc agaggaatac ttttgatttc tgcaatcacg
    17701 atagctggtg gcatcctaat ttcaataatt aataaatttg atttcataaa atatttaatt
    17761 atagcaattg cttgtgtcgc tgcaattata tttagaaaac gattgctact tttagtaagg
    17821 agaattatga gcaaataaac atttgctcat agagagatag agaatgatag atattatttc
    17881 tgctataatg gtaaatgtat taacagcatt atatcagccg ttcttatttg ccgtattaac
    17941 agcgtatctg tttatgtact ggtatctatt tatagttgat aactctcgag atcctaaaaa
    18001 cttaaaagat tctgtattaa tctggttgag atattttaaa gcatctagta aatttcgcag
    18061 actctttttt cttgcgtttt attttacttt gatattattt aaaaccttgc ttaatcgaaa
    18121 tatgtggatg aacccgcttt caaacgttat tggacagtgg tcattatata ttatagataa
    18181 tgctacagga gagagcagat tgacaacaga atgcgtggag aacacgatgc tgtttatccc
    18241 tttttgcatg ttattgttat ggtggcgtga cattaaatgt gatattatac gcaccatcta
    18301 tgttgggatt aagtatgtgt ttttattctc attaagtata gaatttacac aactgttttt
    18361 taggcttggt acatttcaac tatcggattt gttttataac accctagggg gcttaattgg
    18421 tgctttgcta tattggctat tctaccgatt aaataagtat atagacttaa aatagaactg
    18481 ctttgaattt taatatggtt aatatttttc tgcagacagg attgtgtaat ctcatcaaga
    18541 atactttaca tgccatctat ttttgctaaa acattgttgt tatatcttca agaggtaggg
    18601 aattttattg ctttaaatga ggtcgagcga attaaaaaat tggtaaaata tataacatgg
    18661 tggtagttat ggtaaaagtg tttgatataa ttaagttttc ggatttcaga ggatatactg
    18721 ctgttgcatt ggataatgag actcttttct cgattggtat tgagtttcac attaagcaaa
    18781 taaatcaagg atttagtcat aataaatata ctatgcgagg gctacattat cagagtggag
    18841 agcatgctca aggaaagata gtgatgtgcc tacatggtga aattatgaat atcgcacttg
    18901 atattcgaca aggatcttcc acttatggat gtgtgtatgt caacacattg agtgaaagca
    18961 atggtaaatg tgcgtatatc ccacgtggat atgctcatgg ctatcttact atggaggaca
    19021 atacgctagt tcagtggtgc gtagataatg atttcaattc taatgctgcc aaatgtataa
    19081 gttatgaaag cgtagatatt gaatggccgg tcagtcggca gtgtttcgtt ataagtgaga
    19141 aagacaaaaa tggggaaaga ttaagctttt gattaaaatg agctgtggag aaaagaagga
    19201 tactggaaaa taagaagaac aataaccgag caaatgctcg gttatttcta tgaggaatac
    19261 ataaaactaa atttagatat agatatttat tcacgaaaaa ctaatattg
//
LOCUS       DACEQH010000050        18840 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.051, whole genome shotgun sequence.
ACCESSION   DACEQH010000050 DACEQH010000000
VERSION     DACEQH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.051"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(21..251)
                     /locus_tag="IW833_001966"
     CDS             complement(21..251)
                     /locus_tag="IW833_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308069.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="IW833_001967"
     CDS             complement(687..1253)
                     /locus_tag="IW833_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1308070.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="IW833_001968"
     CDS             complement(1253..2209)
                     /locus_tag="IW833_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ1308071.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="IW833_001969"
     CDS             complement(2209..2865)
                     /locus_tag="IW833_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1308072.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="IW833_001970"
     CDS             3235..5253
                     /locus_tag="IW833_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1308073.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5680..8094
                     /locus_tag="IW833_001971"
     CDS             5680..8094
                     /locus_tag="IW833_001971"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ1308074.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="IW833_001972"
     CDS             8442..9995
                     /locus_tag="IW833_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1308075.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="IW833_001973"
     CDS             complement(10376..12160)
                     /locus_tag="IW833_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1308076.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="IW833_001974"
     CDS             complement(12175..13896)
                     /locus_tag="IW833_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1308077.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="IW833_001975"
     CDS             13996..14757
                     /locus_tag="IW833_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1308078.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15724..15992
                     /locus_tag="IW833_001976"
                     /pseudo
     CDS             15724..15992
                     /locus_tag="IW833_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16664..>18840
                     /locus_tag="IW833_001977"
     CDS             16664..>18840
                     /locus_tag="IW833_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308079.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
BASE COUNT         6185 a         3343 c         3772 g         5540 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gtttttttat
    15361 agaaatttta aaaaataaaa cacgttattg gtggatagtt agtttgttat ttggtaatat
    15421 tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt tattttttgg
    15481 aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa aaagcagtat
    15541 gactaatata aatactattg tcatttatgc aattttttat ggatagtaat tttagagggg
    15601 aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata
    15661 gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga atcaactaca
    15721 acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga aaaggtagtt
    15781 attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac cctttgcaat
    15841 atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat ttaatacaga
    15901 ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct aagaagatga
    15961 ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc aaataaagag
    16021 caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg gtggacagtt
    16081 tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc ctaaatgcgt
    16141 ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc gatgaatccc
    16201 aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt tttgggattg
    16261 atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac cttggtatca
    16321 acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca ttaacaaaat
    16381 aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc gtgaaatcat
    16441 aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa tcaatcaaca
    16501 gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct tgagttgaac
    16561 cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt agaaaattat
    16621 cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga acaaatttca
    16681 taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg ggaacataac
    16741 ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg aagttgatca
    16801 agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac cagacctgct
    16861 aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga agcaactcaa
    16921 tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg aattgaccgc
    16981 aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac cagaagaaag
    17041 cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta atataccaga
    17101 agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta taacgcagga
    17161 atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct ctgctgagcg
    17221 ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag gaggcgctgg
    17281 cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat cgattggctt
    17341 aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac tggcatttta
    17401 ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat taaatgaagc
    17461 cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat caggcggtac
    17521 tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag caaacacgga
    17581 attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat actatggctc
    17641 tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc aagacgtgac
    17701 caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt
    17761 ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta catggagttc
    17821 cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg
    17881 tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag tatcttttaa
    17941 taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc aatcgtattt
    18001 tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa gacaagagga
    18061 tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga gccgagacta
    18121 ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa
    18181 aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct cgatgttttc
    18241 tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc
    18301 atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa tagttcgttt
    18361 cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta aggtcaatgt
    18421 agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga atagtgacaa
    18481 tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta aagtaattaa
    18541 aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat caagcgattc
    18601 acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg aaccgcttcc
    18661 tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt tagatgcgac
    18721 ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa ccattgaaaa
    18781 catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg gtgaagtcct
//
LOCUS       DACEQH010000051        18717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.052, whole genome shotgun sequence.
ACCESSION   DACEQH010000051 DACEQH010000000
VERSION     DACEQH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.052"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            99..746
                     /locus_tag="IW833_001978"
     CDS             99..746
                     /locus_tag="IW833_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1308080.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IW833_001979"
     CDS             1139..3784
                     /locus_tag="IW833_001979"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ1308081.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IW833_001980"
     CDS             4094..5407
                     /locus_tag="IW833_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ1308082.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IW833_001981"
     CDS             5397..6050
                     /locus_tag="IW833_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1308083.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6776
                     /gene="radC"
                     /locus_tag="IW833_001982"
     CDS             6105..6776
                     /gene="radC"
                     /locus_tag="IW833_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAQ1308084.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(6671..8023)
                     /locus_tag="IW833_001983"
     CDS             complement(6671..8023)
                     /locus_tag="IW833_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1308085.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(8089..9378)
                     /locus_tag="IW833_001984"
     CDS             complement(8089..9378)
                     /locus_tag="IW833_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1308086.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(9465..10169)
                     /locus_tag="IW833_001985"
     CDS             complement(9465..10169)
                     /locus_tag="IW833_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAQ1308087.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            10369..10635
                     /locus_tag="IW833_001986"
     CDS             10369..10635
                     /locus_tag="IW833_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1308088.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(10803..11033)
                     /locus_tag="IW833_001987"
     CDS             complement(10803..11033)
                     /locus_tag="IW833_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1308089.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            11105..11260
                     /locus_tag="IW833_001988"
     CDS             11105..11260
                     /locus_tag="IW833_001988"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308090.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(11569..11793)
                     /locus_tag="IW833_001989"
     CDS             complement(11569..11793)
                     /locus_tag="IW833_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1308091.1"
                     /translation="MGKNQHVVPNSNGGWNVKDAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            12060..12395
                     /locus_tag="IW833_001990"
     CDS             12060..12395
                     /locus_tag="IW833_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308092.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            12628..13569
                     /locus_tag="IW833_001991"
     CDS             12628..13569
                     /locus_tag="IW833_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ1308093.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            13571..14461
                     /locus_tag="IW833_001992"
     CDS             13571..14461
                     /locus_tag="IW833_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ1308094.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            14504..15304
                     /locus_tag="IW833_001993"
     CDS             14504..15304
                     /locus_tag="IW833_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1308095.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            15319..16170
                     /locus_tag="IW833_001994"
     CDS             15319..16170
                     /locus_tag="IW833_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1308096.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            16167..16526
                     /locus_tag="IW833_001995"
     CDS             16167..16526
                     /locus_tag="IW833_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ1308097.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            16541..16702
                     /locus_tag="IW833_001996"
     CDS             16541..16702
                     /locus_tag="IW833_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1308098.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            16699..17025
                     /locus_tag="IW833_001997"
     CDS             16699..17025
                     /locus_tag="IW833_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308099.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            17022..17837
                     /locus_tag="IW833_001998"
     CDS             17022..17837
                     /locus_tag="IW833_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAQ1308100.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            17976..18287
                     /locus_tag="IW833_001999"
     CDS             17976..18287
                     /locus_tag="IW833_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308101.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            18284..18514
                     /locus_tag="IW833_002000"
     CDS             18284..18514
                     /locus_tag="IW833_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308102.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            18521..>18717
                     /locus_tag="IW833_002001"
     CDS             18521..>18717
                     /locus_tag="IW833_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ1308103.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEE"
BASE COUNT         6311 a         3096 c         3801 g         5509 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaagaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tcttctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttccggaata tataagttag
     6661 tacacataaa ttatataagg aaatttactg ttatattgcc atctggaaat actattatgt
     6721 tatcgataat tgaacgccat aatgcatttt tttcttcttc cgaagattct ttataaattt
     6781 catcgaattc ttttgataga ataaattcga cattttttat atctatttta gctggctcta
     6841 cttcaatttc atttaattct ttcattaaag cttctcgatc ttttttgtat tcctctaagt
     6901 ctataacttc attcaagtag gctttcttca atctatctaa tttattctca atatttttct
     6961 tactattttt ctttttaata atctttggtt gtttttttga atagtttatt tgtatttgtt
     7021 caaatttttg tttagtagct tcaaaaatat aacgttctaa atttttttcg taaaaagcgc
     7081 gcttatttgt gcaacgtaat ttattatttc ttcgttttac acatctataa caagtccttt
     7141 cgtatataga ttctgtaccg tcttttttct tatatcttgc tttaatagtt tgaccagcta
     7201 ctgatccctg acagtcagaa cattttaata aacctttaaa aatgtaatca cgttttgcat
     7261 ttgtttttaa atttttaggt aaggcttttt gaacagcata aaaaagtttt ttatcaataa
     7321 taggaggaca gtaattttta ttatttctta attcaccaat gtattttttg ttcgtcaaca
     7381 tatttttaac actttgataa tctctaacga ttccatactg agagcctaaa taataaacag
     7441 tttttcttaa actaccacta gaaagaaagt agttgaatat atctatcacg ataggagcat
     7501 catcatttat tactaatttc ttattttcta tagagtagcc taacggagtt ttcccgctta
     7561 ctacttcacc ttgtgcaatt ttatttgcca taacagcttt gatgcgttct gaatcaattt
     7621 gggcttccaa ttcagcaaaa ctcattactt gtgcaatgaa agttctgcca tacgctgtag
     7681 ttgtatcata atattgttga gaaacagcat tccaagaaac attgtgtttt tctaaaattt
     7741 cttgagtgtt taaataatgt cgtagatttc taaaccatct atctaatttt gtaaataaaa
     7801 ttagatctat tttattttct tttacatctt gtaatagttt ttgaaattca tcacgattta
     7861 ttttttgacc agaaatacca ccgtctacat attcattaac tactttcaaa tccttgtgtt
     7921 gttcgatata ttcatagagg gtttcttttt gttctctaag actatctcca tgtttggctt
     7981 gttgttcagt tgatactctc atatataatg ctactcgttt catgttatct cctttcgaat
     8041 gtatgttctt atcgttgtaa aagaaaagcc cgaaggctaa tctttttact acacatctgt
     8101 taattctaat gatcttctgt attgttcagc ttgagctagt ttagtaaaag atacagttcc
     8161 gttataattt tctaaaacaa ctttttcaat ttcatcaaga gatactttaa agaattcttt
     8221 tcgttgatta actttattga cttcgttttt tctaaaatgc gaatgtagtt gattttctaa
     8281 tgttggtgca tcttctgaaa atatcattgc atgaacatca aattcaaaag gtacagaagc
     8341 gctactaagt tccttaattc tatccatagg ttctaaccgt ctagtcattc ctattttata
     8401 gacatcttca ccgaaagatc cgatattact gatgacataa acatagccag ctctagtatt
     8461 aagttctctt tgcaatacat cttctttgac agtttctagt tctcttaatt tctcttccag
     8521 ttctttaatt ttatcagcat agagttcttt ttcaacatca gcactagctt tttgcatgta
     8581 tgccattaac ttagttatct ctgagttaaa ctgtcgttct tctttatcaa tgcgtttctt
     8641 ttcacgttct atttcacgtc ttaccttttc ttcttcaatc atttgttctt ttattgcttt
     8701 ttgctgttct ttttcatcgt taactttaac tatgtatgaa tagttgagtt ctaattcttg
     8761 aagttttaat tcaagtaatt cttttttgaa ttctgcttca tcgtttttaa aaagcttgtt
     8821 tattttatca aaagttctta taattttttc tctagttgaa tctacgttag aaatagtaag
     8881 tttattgaga agtccagtta tttctgattc taatggtata agcattttct ttttaatatt
     8941 attagcttct ctattattca aataagaagg aatattaatt gcaccgtcta aaataaaatc
     9001 cttttcttta atctttaata aagaaagttt gttttttatt tcggatgaag aagtttcatc
     9061 tatattcgga ataattactg atttatagat agtgaccaga gatgtgtcat atttttgatt
     9121 aatttcatcg tctaaccgct ggctttcagc attaagaata tcaattttgt tttctagcgt
     9181 tgacaagttt ttattcaatt tacttatgtt tttctctttt tcagatataa acgtttccct
     9241 agcatttaat tcttgagaat attcctttac cttactctct aaggcagaag ttttatcttt
     9301 taatttttta gcttcagata atttgctact acccataatt attaaaacta ttccaactaa
     9361 aaacaatagt actcccattc aaaacgcctc gcttctttta taaaataatt gtcaaaatca
     9421 tataaaccct taccgaagtt gacggctccg gcatgggaat ttttttaaaa tacaactttt
     9481 ccgataattc taatctgttc tggcgataca attctatctt cgtataagtc gtttatagaa
     9541 cgtaaaatga ttgtttgatt ttcgtaatct ttatatatct ctttacatgt aactccgtca
     9601 ccacctaatt ccacaatagc aatttcccca tcttctacgt ccggttgttc tttaacgaat
     9661 acgaattgtc cattcctgat aacaggttcc attgaatcgc ctataatagg tactgcgaag
     9721 tcagcagcac ttggaactct ctcaaattct tcctcttcga caaccgtatc accgtaagta
     9781 agttctgttg gattggcagc cgaagccgaa ttgtgaacag ttgggatttt ttctttttta
     9841 ggaaaatcta ggacattttc ttgtttttgt tcttctaatc gattacttgc aaattcatag
     9901 gtagcaactt tattttcgtc gttaagctga ttatatatag gaaggatatt atatatctga
     9961 ttagatatag ataaatcaat attcatcaaa tcatctatat caacattgaa tatcttagct
    10021 atctcattta aggtttttaa ttttggtgta tattttcctt tttcccattc gcttattgag
    10081 gatgaacttt ttcttcctag tttttcggct agttcttgtt gatcaatatt atacttttct
    10141 ctaagaaact ttagattttc agcgaacata gtaacacctc cttgagttaa ttgtaacacc
    10201 gtttcggaaa aaatgtaaca ggtttcttag aaaatatttt agtgttttta aagaagtatt
    10261 tacgcaaaat agaaatcgat ttcaggaaaa ctgaaatatg cacttgactt cggaaaaacc
    10321 gaagtatata atttagtcag aaagttaaag agaggaggcg aataaaatat ggataccgcg
    10381 aagaaagaaa aatatacatt gagacaatta agagctttaa agggttattc aaaagaagaa
    10441 ctgtcaagga aaagcaaagt gacatcaaga acaatattta tttacgaaaa cgatgtagat
    10501 aaaatgagga acggaaagta cgccactcta gaaaaaattg ctaaggcatt gggtgttaga
    10561 gtttccgata tttttttaga ccctgattcg gaaaaaccga aatcaataac aggtgaaaca
    10621 aaacaagtca gctaggaggt gtgaatagtg gaagagaaga atataaaagt accgactctt
    10681 gaagaatata caaaaattgt tgtcgaaaca gacgagcaga acccgaaaac aatcgcagtt
    10741 attacggcga atgatattga atcatgcgaa ggttttcgag ttcgaatgac ccctagatat
    10801 gattaatgtt ctttatcacg tggaggtata ggatcgtttc caaaactatc ttttgattgt
    10861 atttgtccat tctttttttg gacaataaac tctgattgct gttttttaga aatcttttta
    10921 ccatactcaa cagcatctga tttattatca aatagttttg tatcttcgga attaccttca
    10981 ccacgtacag cccatttgcc atttctagga gatacccatt gatttttacc cataacaatt
    11041 cacctcgctt tcaagttaat tctattaaga ggtgaaaaga aaacaaactg atggaggtga
    11101 gattgtgagt aacaagccaa aaatcacttt gaatggcaca cctaatcttc aaagattagc
    11161 agatgaaatg gctagaatac gttctagaca ttggggagtt actgtggtgg caaccataat
    11221 tgaacaaaaa gaaaagccca cacagccggc aagcaagtag tgggcaccat agaacaataa
    11281 tattatatca cacactcgcc acttccgaaa agaggagttg ctgctctcct ttcaagtttg
    11341 ccaaagtcca cgtttcttca ttttggcaac tcctcttctt ggaggtggtg acggcgagag
    11401 acgaaggagg gatgacatga aggaagaatc aaaaataggg attttgaatg agaacaatta
    11461 cacaagatta attattgaaa cagaagaagg ggaaaaagta gcagaagtta cattgaccga
    11521 tgcaactcct gctgacggtt atcgtattcg attaactcct aaatatgatt aaccttttgg
    11581 tgggtgtgga tcgtttccgt gactatcacg gctttgaatt tttccatctc tgccatgaat
    11641 aaatagctca gatccttgat ttttagaaat ctctctagcg atttttactg cttctgattt
    11701 tgtattagta tgtgcagtcg ctttagaatt tccagcatct ttgacattcc aaccaccatt
    11761 ggaattaggt acaacatgtt gattttttcc catagttttc acctccattt ctatagaaat
    11821 attttatcaa atagaaatgg agaaattcaa taaacgaaag gagggatcaa atgacattac
    11881 aagagcatca acacttgatg cgaaaactaa atcaggaata ccacgaggaa atgaaaccta
    11941 gattgctagg ggaacaatgg cgtgaacgcc agaagaagtg gttagaaata aaaaagtgac
    12001 tcagccgtcc aaagcaatga gtcacaaacc aaaaattata cctaaggaga ttttatcaca
    12061 tgaataagaa aattgaaaat cttatcgaag aattgaaacg tgaatgccaa aagcaaggtg
    12121 tttctattat ttgtacagcc caaaaagaag gggaacttaa aagtctcgtt tatggtgaga
    12181 caacagaaat tttactttgt cttgctatgc aagaagaaca tttagatgaa aatctcccat
    12241 tgtcagcaca tattatgcgt agaattgctg tggatgctta cgaacaagcg aaaaatgaag
    12301 aagaaaatca accttcaaat catacttttg ttattaataa caaggaagat ttagctgatg
    12361 tgatgactcg tattctcaag ggggaatttc aatgatgaat gaaaaaacaa ttgatctact
    12421 cattatttta tttttcacgt tagtgattgt agcgaaaagt tatgtaatct ttggcttgtt
    12481 attcgcaatt accatgcttg tgctttgcgt gttagcaagc agaaaagagg ttaaacatga
    12541 acgagttagc aaatcttgac aactatttaa ctgatcctga ttatgctaaa ccaccatatg
    12601 aagcgccaat tgatgaggag gatgaagatg agtaaatcta ccttagaaat gagccatcaa
    12661 gaatggctcg aagaccgcaa aagaggtatt ggtggctcag atgttgcaac cgtacttgga
    12721 ttaaacaaat acaaatctcc ttatcaattg tggcttgaga aaacgggtca aattgaattg
    12781 aaagatttag aaagcgaacc agcttattgg ggtaatgttt tagaagaagt ggttgctaaa
    12841 gagtttcagg aacgtacagg caaaaaagta cgcagaagaa accaagtatt tgaacatcca
    12901 ttacatccat ttttaagagc aaatattgat cgggaagtag tgggagaaaa tgccattctg
    12961 gaatgtaaaa cagccaatca atttctcgga aaagagtggg aaggtgaaga ggtaccgctt
    13021 agctatctct gtcaagttca gcattacatg aacgttttaa acaaagacta ttgttatatc
    13081 gctgttttga tcggtggaca aaaattcatt tggaagcgga ttgaacgaga tcaagagctg
    13141 atcgatacaa tcactgaaca attagtagag ttttgggaaa cgaacgttct tggaggtatc
    13201 gagcctgtaa ttgacggaag tggagcgact gctgacttct taaaagaaaa atatgcagat
    13261 gtagaagaaa atcaaacagc tctaccatca cgttttgatg aacttatcga gcaaaaaaga
    13321 gaactcaagc ggacgaaaaa agaaattgaa tcatctattc gtcaagtaga caacgagatc
    13381 atcagtgagc taggtaaacg tgaagccagt atcggtatca cacaaagaaa cattatcagc
    13441 tggaaacttg ttcgtacaag acggatgaac acgaagaaac tagcagagaa atatccagat
    13501 gtcgcaaatg atgaagagat ttataacgtt actgaatcaa gaaggctaac cgaaaaggag
    13561 atcaaataat atggcaacaa atgaatcgtt aaaaaatcaa ttggcagaaa agccacagaa
    13621 acaagttgca ccaggacagt tagggcttaa agctctaatg aatacaccaa caatgagaaa
    13681 gaaatttgaa gaagtacttc atgacaatgc taatgctttt atgtcgaatg ttatgactct
    13741 tgtatctaat gacagttatc ttgcagatag tgaaccgatg tctatcatga gtggtgcgtt
    13801 aactgctgca acattaaatc ttgggctaga taagaattta ggttatgcat atttagttcc
    13861 attcaatagt aaaaacaagc aaacaggaaa atgggaaaag aaagctcaat tcatgcttgg
    13921 ctataaagga tatatccaat tagcccaacg atcaggtaaa tacaaagcat taaatgtgat
    13981 cgaagtttac gaaggagaac taaaaagctg gaaccgactg acagaagagt ttgagtttga
    14041 tccaaatggt agaacgtctg atgaagtcat tggatatgtt ggctatttcg agttactgaa
    14101 tggattcaaa aaaactgtct attggaccaa acaagaaatc gaagctcatc ggattgctaa
    14161 caataaagat cgcgataaga caaagttaag tggtgtgtgg gcatctgatt acaatgcaat
    14221 ggcacgaaaa actgttttga gaaatcttct ttctaaatgg ggaatcttgt ccatcgaaat
    14281 gcaagaagcc accacatcgg atgagagagt ccaaagggtt caagaagacg gcagcattat
    14341 tgctgaaaca gaagttgaag aagatattcc tgaaagaaaa gaagcagagg ttatatctga
    14401 agaaaatgaa gatgtacaaa ctggattatt tgatgcatct aatccgccgt taaacaaata
    14461 atgagggagc tttctccctt gcttttctag aggagaaata cgaatgaaca caggatatgt
    14521 caaattacat cgaaaagtga tgaattcatt cgtttggacc aatccttata tgtataaatt
    14581 atggagcttg tgtttgatga aagcgagtca tgagaatcgc aagatacttt ttaatggaaa
    14641 agagatacaa gtgaacagcg gagaattcgt cacagggcgc gatgctatca catctgagat
    14701 gaacgaaggc gtgaaacgtg aacatcaagt gaacagcggt tctgtatgga gatggctaaa
    14761 acaatttgaa aaaaacggaa tgttgaacat caaatcaact acgaaataca gcgttatatc
    14821 aataaaaaac tggtctttgt accaaagcag tgaacaacaa atgaacatca aacgcacaac
    14881 aagtgaacaa caagtgcaca caaacaagaa tgaaaagaat tataagaatg aaaagaataa
    14941 taaaacatca tcgtcgcaac cacgcaaaaa gcgtgtttac gacaccgact cagtttacta
    15001 cattctcgcg gaggagttat tcaaacagat ttgtcagaat caggaaatca aaaagccgaa
    15061 tctgcaaagt tgggcagata acattcggaa aatgatcgag atcgacaagc ggaccgaaaa
    15121 ccaggtacgg ggaatgattg aatggagtca gcacaatgtg ttttgggcat cgaatatttt
    15181 atctgctaaa aaattgcgag aaaaatacga cacaatggca gcgcaagcaa atcgtgatta
    15241 taaaacaaaa caaactaaaa cgcttgaata cgagaaattt agcacagatg agttgcctat
    15301 ttgagaggag gcgtaggcat ggaaactgtt ggggaaatca tggaaaagct gatacagaaa
    15361 gtgcttgtcc aacgtggcga atgtcctgaa tgcggacagc ctttgtatgg atggcgtacg
    15421 aagaaccctg atggctcaga acgttgtaag ccaacctgta tgcaatgtgg gtataaagca
    15481 ttgcgagttc aggaagactt acaaacagaa cgaatttaca acgagagcct gaaagcaaga
    15541 gcaatcaatt ttttcaaagg tggttctgtt gtgcctaatc aagcgttgtt tgattgcaca
    15601 ttgcagaatt atcaaattgt cgatcaagaa acaagacaag cggttgaagt aaccaaacgc
    15661 tttgttaatt cagtcttgtt aggaaatcca agtcaccttg ttttaactgg aaaacaagga
    15721 acaggtaaaa gtcacctagc gatggcagcg gcttgggaag tcttgaagcg atcaaactac
    15781 gataaaaaaa tcttatttat cgggttacag gaaatgctgg atcaaatcaa attttcgtac
    15841 aacaatcctg aactcagaaa aacgattgag ggatcgttga ttgcagatat caaaacagcg
    15901 gatttagtca tcattgatga tatcggttca gaactaggaa aagatgcatc agatagtcga
    15961 gcgtttggca taaacacgct aaattcgttc ttggatgcac gacagaacct agcaacgatt
    16021 atcacaacga acttgcttgg tgaagaactg aaaaaagctt acggtacgag aacaatatca
    16081 agaatgtttg tcaactctga tggatttacg atggtatttt ctcaaacagc agacaagcgc
    16141 ataaaaccag tgaaaggtag tatcgcatga ataaataccg taataaaaaa actgttcatc
    16201 gaggtatcaa gtttgattct atcgcggaag cagagtatta cgatctagcc ttgtggcaag
    16261 ctgaagcgaa tggttggaaa gtgaagctcc aggaaaggtt tgagctgatg ccgaagtttg
    16321 aactagaggg caagaagtat cgcaagatcg agtatattcc cgacttcaca ttttacaaaa
    16381 gcggcaagct ggtcaaagtc gtagatgtta aggggatgca gacaaaagac tttaagatca
    16441 aggcaaagtt gttctgtcat caatatcaag tgccgttgat attagccaaa aaatatcgga
    16501 atacgttcaa ggaagagcgt ttttaacgag gtggtccatc atgacaacag aagaagtgat
    16561 tcaaatgcga attcgaagcc ttcggcgtga gattgacgat ctggaacgaa caaaggcagt
    16621 gatggtcaat gaaacggcta gaaaggcaat cgatttgcac atagagaatt taagaaggga
    16681 aatccgtaga ttggaggaat gagcgtggat aaggaagcgg cttggcgaaa attaatgttg
    16741 ctgattcaag atgagaactg gcaagaagat gaagcagtgg ttgctgaagt tcagcgtcta
    16801 ggaaagattg ctaacggacg tatacgaaaa aagccagaca aaagaaaaca gcgcaaaggg
    16861 aaaatcgtcg ttgttttaca cgaaggcaaa attttgatgc aaggaacagc tagtgagctg
    16921 tctgcagaaa ctggatatac gcgtgggact attcgaacgt acgcttggcg aaatcacacc
    16981 gatcgaaaag ggcacgaata taagtatttg gaggaagaaa aatgaacgaa aacaaattaa
    17041 tcaaattggg tgtagcagga gcagtaatcg taggtattgg agttatcgga ggatttaagt
    17101 tcttcgaaaa aatcgataat ggatatgtgg gtgtgcgcta ttcaatgaac ggcggtatca
    17161 aagatgaagc actgacgcaa ggtgtgaaat ttgtagggat tgacaaagtg atccaatatc
    17221 caattcgctt gcaaactatc caatcaaaaa atatttcagt atctacaagc gacggcaaaa
    17281 agacaacgat tgatatcaaa tatgactaca aagttgattc aactaaagca gcaaaaatgt
    17341 acaaggaatt tgggaatatc acttcggaag atatcgaaag tggatggtta aaatctaagc
    17401 ttcaaaaggt cgctcgtgaa gtttatgcga aatatagtct gcttgatgtc ctttcaggag
    17461 attcctctaa agttgaagct gaggtattaa cgaactttgc taaatcagtt gaatctaaag
    17521 ggtttgaagt cgaagacgta acacttggtg ttccagatgt cgataaagaa acacaaaaat
    17581 caatcgatgc gatcattcga gctggtcaag aaaatgaaaa agcgaagcta gacgcagaaa
    17641 ctgcaaaaac tcaagctgat agtgaagctt acaagaaaac aaaagctgca gaagcagagg
    17701 cagaatctaa tcgcaaagtc gccgaatcag taacagacaa tttgattcgt tatgaagaag
    17761 ctcaagctcg caaaaagcat ggatgggtaa cagtaaatgg agcagatact gtagttacgg
    17821 atgaagcagg caaataatat gggattcttt atggctaaaa ttctcttgtt cttaggttta
    17881 gtaggagcag catatctcgt gtatgcgctc ctttcccaaa ctgatgacaa agaagatgac
    17941 aacaacgatg aaatgaaatt ttaggaggag aaataatgga cgaactaatc acaaaagtag
    18001 agcagtgggc taaagacaag ggactggatc aagcagatcc aaaagcacag tttttgaaag
    18061 tagctgagga attcggggaa atagcttcgg cgatggcaag aagtaatgat gaactattta
    18121 aagatagcgt aggagacgtt atcgtcacgc tgattatcct ttccatgcaa aaagggacaa
    18181 acgtacaaga gtgtttagaa atggcataca acgaaatcaa aggacgcaca gggaaaatgg
    18241 tagatggtgt attcgtgaag tcgagtgatt tggaggatgt gaaatgaaat acgaaatacc
    18301 actaagtgaa gcgggcattc aagcaattat caatggtcgg gaggttaaca tagaacttcc
    18361 tgatggtact gaattagtca tcagacaaag ttatttgaaa gatatggcag ctccagtatt
    18421 aattgatcgt tttaacgtga ctgattctgt ggtagagaac cacttaaaag aatttcgatc
    18481 aagtatagac aacactttca gattagggag ttgattgaca atgaacacca gacaccgcag
    18541 agtagcaaaa ctaagaaaac aggaactaaa tgtactaaag acaaagtttg aaaaagaata
    18601 tggaatttca gcagaagaaa catataaagt ggcaagtcag tttgttgctg atgcaagtga
    18661 tgctattcgt aagtttggga tttcgatatt aaatgatgat cgtaaatggg aggaaat
//
LOCUS       DACEQH010000052        18112 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.053, whole genome shotgun sequence.
ACCESSION   DACEQH010000052 DACEQH010000000
VERSION     DACEQH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.053"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(24..722)
                     /locus_tag="IW833_002002"
     CDS             complement(24..722)
                     /locus_tag="IW833_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ1308104.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="IW833_002003"
     CDS             complement(715..2067)
                     /locus_tag="IW833_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ1308105.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="IW833_002004"
     CDS             complement(2290..3690)
                     /locus_tag="IW833_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ1308106.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="IW833_002005"
     CDS             3851..4588
                     /locus_tag="IW833_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ1308107.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="IW833_002006"
     CDS             4713..5192
                     /locus_tag="IW833_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ1308108.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="IW833_002007"
     CDS             5189..5956
                     /locus_tag="IW833_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308109.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="IW833_002008"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="IW833_002008"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1308110.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="IW833_002009"
     CDS             complement(6859..7461)
                     /locus_tag="IW833_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1308111.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="IW833_002010"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="IW833_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="IW833_002011"
     CDS             8142..8726
                     /locus_tag="IW833_002011"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ1308112.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="IW833_002012"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="IW833_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ1308113.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="IW833_002013"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="IW833_002013"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ1308114.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="IW833_002014"
     CDS             10627..10806
                     /locus_tag="IW833_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308115.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="IW833_002015"
     CDS             10828..11853
                     /locus_tag="IW833_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ1308116.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="IW833_002016"
     CDS             12028..13272
                     /locus_tag="IW833_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ1308117.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="IW833_002017"
     CDS             13425..14372
                     /locus_tag="IW833_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1308118.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="IW833_002018"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="IW833_002018"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ1308119.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="IW833_002019"
     CDS             complement(15274..15516)
                     /locus_tag="IW833_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308120.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="IW833_002020"
     tRNA            15637..15709
                     /locus_tag="IW833_002020"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
     gene            complement(15828..16466)
                     /locus_tag="IW833_002021"
     CDS             complement(15828..16466)
                     /locus_tag="IW833_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1308121.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16589..17221)
                     /locus_tag="IW833_002022"
     CDS             complement(16589..17221)
                     /locus_tag="IW833_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1308122.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17572..17871
                     /locus_tag="IW833_002023"
     CDS             17572..17871
                     /locus_tag="IW833_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1308123.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6209 a         3162 c         3568 g         5173 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat
    15781 aaagtatgct ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa
    15841 acgactagca atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc
    15901 gagttgcttt taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg
    15961 atgtgtgcta cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca
    16021 aagatgattg ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg
    16081 agaaaataca ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat
    16141 aacgggctga aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc
    16201 aaaaataccg agactgcggc actgattact acattgataa aaaaatattt ggctatattt
    16261 ttgacaggag cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt
    16321 gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat
    16381 agcaacccta acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct
    16441 attttttttc ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata
    16501 gagtcagtat acatgaagag ttaaaatagg caatgttttt ttatggatat ttgtattgct
    16561 aaaaatttat cttatatatt cattttgatt atactacttt tatttgtaaa aattttctag
    16621 caaaaccaag tgcttgcaac tgattaagcg tacaattttt tagatgttcc gagcgtactt
    16681 caatcatttc aaaggagttg ctacttaaat cgatattttt taaatcagta tcaaaaaagc
    16741 tagcattttc taaatcatta gtagaaaaca ttattttagt gagggaacag tctacaaaat
    16801 ctgcgcttga taagacagag tgctcaaagg tactttcttt tatgtttgaa ccagataaat
    16861 ttacatagtc cattttacaa ttctcgaata acgtgttccc aatataactt tccatcaatt
    16921 tagtacctgt taatttacaa ttgacaaatt tcacgcgatg caacatgctt tttgtcaata
    16981 tacagcctga aaaatcacag ttttcgaata tacagtcggt aatctccatt ctttgaaaat
    17041 cattggaagc aaaaaatgca tacttgaaaa tagatttgta aataattgga tggcagatta
    17101 tttggttttc gatgacctct ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt
    17161 catagatatg aaaaaaggat cctttattca agcttgcttc attaacttta ggaaaaatca
    17221 tatttaatta cctccatttt cataaagtca tattaacaaa agctaaacca acttacatta
    17281 gcagaaaatg agataggttt tcgccttttt cgataacgat agtgtcgcta tctcttgaaa
    17341 cgttcttaga attgccctga gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa
    17401 taaaaatttt tactgattta atttagaatg tttttaaaat aaaaatcgtt gccataacag
    17461 tgtattgaaa ggttttaaca atccagtaat gatttaaaaa aatctagatg tagcgtatac
    17521 tttgtttata gaatgtagtg ggaagaaata tatttttggg aggaaatcgt gatgtctaaa
    17581 agtgaagaaa tagaaaacat ggtactaaaa ttatatgatc tcgttttaaa tccagagatc
    17641 aaagaaaagg aacgtatact tttgatagac gcaaagacag gcttagaaaa aggtcagtat
    17701 tatcctaaag tcataaataa tttggaacgt agtttacgtc cgttagcaat caggggagaa
    17761 ttgtctaaac cagtatcacc gttttatatg gagatatcaa caataggaaa atttgaaaaa
    17821 gaattaggtc gaggaatggc ttcggcaccg attacttttg ggcatttata aaaacgagtt
    17881 attcatctca atgaattaaa aagtatagtc aatctcataa tgcaggctag gttcatttat
    17941 ttgaacagag tctgtatttt tttattctaa ataaagaaac agatgtttct ttatattgta
    18001 ttttatcctt taattaacac aaaaacagct agtataaact aataatgtta attaaaagtg
    18061 tattttatga aactatcgtt atttattaat gttgcatttt tgtaagttaa aa
//
LOCUS       DACEQH010000053        17688 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.054, whole genome shotgun sequence.
ACCESSION   DACEQH010000053 DACEQH010000000
VERSION     DACEQH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.054"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(137..1717)
                     /locus_tag="IW833_002024"
     CDS             complement(137..1717)
                     /locus_tag="IW833_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ1308124.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2118..3494)
                     /locus_tag="IW833_002025"
     CDS             complement(2118..3494)
                     /locus_tag="IW833_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1308125.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3622..4740)
                     /locus_tag="IW833_002026"
     CDS             complement(3622..4740)
                     /locus_tag="IW833_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1308126.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4882..5316
                     /locus_tag="IW833_002027"
     CDS             4882..5316
                     /locus_tag="IW833_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1308127.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5394..5927)
                     /locus_tag="IW833_002028"
     CDS             complement(5394..5927)
                     /locus_tag="IW833_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ1308128.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6021..7199)
                     /gene="mutY"
                     /locus_tag="IW833_002029"
     CDS             complement(6021..7199)
                     /gene="mutY"
                     /locus_tag="IW833_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ1308129.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7192..7989)
                     /gene="recX"
                     /locus_tag="IW833_002030"
     CDS             complement(7192..7989)
                     /gene="recX"
                     /locus_tag="IW833_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ1308130.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8096..9478
                     /gene="rlmD"
                     /locus_tag="IW833_002031"
     CDS             8096..9478
                     /gene="rlmD"
                     /locus_tag="IW833_002031"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1308131.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9652..10230)
                     /locus_tag="IW833_002032"
     CDS             complement(9652..10230)
                     /locus_tag="IW833_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ1308132.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10552..11022
                     /locus_tag="IW833_002033"
     CDS             10552..11022
                     /locus_tag="IW833_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ1308133.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11130..12356)
                     /locus_tag="IW833_002034"
     CDS             complement(11130..12356)
                     /locus_tag="IW833_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ1308134.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13110..13310)
                     /locus_tag="IW833_002035"
     CDS             complement(13110..13310)
                     /locus_tag="IW833_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1308135.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13444..14052)
                     /locus_tag="IW833_002036"
     CDS             complement(13444..14052)
                     /locus_tag="IW833_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ1308136.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14137..14607)
                     /locus_tag="IW833_002037"
     CDS             complement(14137..14607)
                     /locus_tag="IW833_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ1308137.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(14655..15569)
                     /locus_tag="IW833_002038"
     CDS             complement(14655..15569)
                     /locus_tag="IW833_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1308138.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            15715..16518
                     /locus_tag="IW833_002039"
     CDS             15715..16518
                     /locus_tag="IW833_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ1308139.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            16696..17643
                     /locus_tag="IW833_002040"
     CDS             16696..17643
                     /locus_tag="IW833_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1308140.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         5364 a         3543 c         2911 g         5870 t
ORIGIN      
        1 agacatttct atgattttaa tatagtaaaa agagccgtca cggaaaatcc gtcacggctc
       61 taaacaaaat atgagaggct gtttttgttt ggctattctt attacttgat gcagggcagc
      121 ttgtgtcaag acttctttac atcaaacttt ttaattttac attgggatat ttatccgcaa
      181 accagcgttc tgcaaattgg ttctcgaata agaacaacgg ttggtcgaaa cggtcacgag
      241 ccaagatatt gcgacttgaa ctcatcttct catccaaatc ttctggatcg atccagcgag
      301 cgattttgtg tcccattgga gacatgatta cttcggcgtt atactcattt aacatacgat
      361 attggaatac ttcgaattgt agctgaccaa cagctccaat gatatattct tccgttagat
      421 atgttttata aagctgaatc gctccttctt gaactaattg atagattcct ttgtggaagg
      481 atttttgttt catgacattt tttgcagtca ctttcataaa cagctctgga gtaaatgaag
      541 ggagttcttc ataagcaact tttagttttc cttcgaacaa tgtatcccca atctgataat
      601 tgcctgtatc atatacaccg atgatatcgc ctgctactgc tttttcgacg ttttctctcg
      661 aatctgccat aaattgtgtg acattgctta atttcagttt tttatttgta cgttccaacc
      721 agacatccat tcctcgttcg aatgttccag aacagattcg gacaaaagcg attcggtcac
      781 gatgggctgg attcatattt gcttggatct tgaatacgaa acctgaaaat tcttcctcat
      841 aaggacttac catctgatca tcttgtgttt tgtgtccata aggagctggt gccaattcaa
      901 cgaaagtctc taaaaaagtc tgtacaccga agttagtcaa tgcagaacca aaaaatactg
      961 gtgtctgatc gccacgagca attttttctt gattaaatgc atctccagcc tctttgatta
     1021 gttctacttc ttctaagact tgttcatata aggaattttc agttagagga tgattttcgg
     1081 gaagcagtcc atcttcatcc aattctacta atcgttcatc ctgataattt tccggacgat
     1141 aaaactctac acgattatgg tagatatcat aaagaccttc tagcccttta cccataccga
     1201 ttggccaatt catcggataa gattcgatat ctaatagctc ttctaattct tctaacaatt
     1261 ccaatggttc acgaccatca cgatctagtt tgttgataaa agtaaatatc gggatgccac
     1321 gtttttttac aacttggaat aatttctttg tctgtgcttc gatccctttt gcactatcga
     1381 ttaccatgac cgcactatcc acggccatca aagtacgata agtatcttca gaaaaatctt
     1441 catgtcctgg tgtatccaag atatttactc gtttcccttg ataatcaaac tgcataacag
     1501 aactagtaac agaaatccca cgttgtttct cgatttccat ccagtctgat ttggcaaaat
     1561 tacctgtttt tttgcctttt acggtcccgg cttgacggat agccccacca aacaatagta
     1621 attgttctgt aatcgttgtt ttcccagcat ccggatggga aatgatcgca aaagttctgc
     1681 gattgtccac ttgttgcttt aattttggat tattcataac ctaactccat ttcttccatt
     1741 caattattta tattttctct aggcacagat tgtccgatct cttaaagaaa gacaatttca
     1801 tatagggctg tgacaaaagc cgtgtagcag tctctttttc cgagtaaacg gcgttctaga
     1861 gacagcctat cagcaataag cccacccaag ccaaaaatac gaaaagctgt ttttgcttgg
     1921 ctgctcttat tacttaatgc aggatagctt tttcacaacc tttgtcaact actacaactg
     1981 aatcagtata gcatggattc agcgaatgaa aaagccacca aaccataaga gtcaaatatt
     2041 tttttgtcag ctgactaaaa gaaaagacgt gttgcttgtt tattcaagca acacgtcttt
     2101 cataaccaag tttccaatta tcttcttggc tcgtcatctt cgaattcttt tctgaagtat
     2161 cttcgttctt cttcaggttc cgcttctgtt tcttccggat catgaaaaat gacacggatt
     2221 ttaaggattc gagaaccttc catttcttca gaaaccagcg taatattatc gacatcaaac
     2281 gataactttt cgccttcatc cggaatcatg cctaatgctg taatcaaata accagccatc
     2341 gtatctacat cactcatatg cagatcactg tcaaaagctt cattgaactc atcaatcagc
     2401 atacgtcctt ggatgatata ttcgtgttca tcgatttttt cataaagatt ctcaacttca
     2461 tctgattcat catcgatttc tccgacgatt tcttctaaaa ggtcttctag tgttgctaac
     2521 ccaacgacgc cgccatattc atctaataaa atagccatct gattctgcgt ctttttcatt
     2581 tcatataaca gatcatcaat aaagaccgtt tcaggaacga ataaaggctc ctgcatgatt
     2641 ttcttgatat ctaaattatc aaagccgaat ttatgggcag cttttagtaa attctttgta
     2701 tgaaggattc cgactacttt atctttgtct tcgttataaa cagggattct tgaatagttt
     2761 tcggataaaa cttcattcac attttcttcc acagtgtcgt tgatatcaat cataaatgca
     2821 tccgttcgtg ggaccatgac ttctcgggca actttcgtat caagagaaaa aaccccttgg
     2881 agcatttcta attcttcatt attcaatacg ccttcgtttt caagcatata acgcatttca
     2941 tctctggtca tttttgaatc ttcatcatca aaagtcatcg gcgtgatacg cgccaataaa
     3001 cttgtggaag cagaaagcag ccagacgaag ggcttagcta ctacaccaat caagcgtatc
     3061 agccctgaag tgaactttgc gacttcttct gatttattta aagcaatacg tttagggtac
     3121 aattctccaa aaacaatcga tacataggtc aaaatagcca gcacaatgat atttgcaata
     3181 ttttttgccg ctgtgccgcc acctaatacc ggagctaacc gttccgataa agtattagct
     3241 agtgaagcac cagacaaaat gttgaccaaa gtgattccta cttggattgt cgataggaaa
     3301 ttatttggat tctgcaatat ttttaaaagc ttttgagatt tcacatctcc ttcatcagct
     3361 ttttgctcga ttcgattctt attgatcgaa acaacagcaa tttcggaagc tgctaaaaaa
     3421 gcattgatca atgtcaaaac gatcaataat aaaatctgcg ctaacagcga ctgactctca
     3481 gggtcagcat tcataatcgc attctctcct tataaaatta atttttagtt tacgatagaa
     3541 aaaaacgtgg cactatagcc acgttaaaat ggtatcacaa agcaattttc tttgcaataa
     3601 aaacaagtgt actaaaacta cctacgaggt cgtagcaatt ttcttcttgt tttcattttg
     3661 cttgtcttct ttgacgattt tgattatata ggaaatgatc gtcttgcaga ttgcgtaaaa
     3721 aggaacacct aagaagatac caagcaatcc ggcaatattt ccagctacta gaagaacgat
     3781 aatgatcgtt aatggatgga ttttcagtga tttcccaatt acattaggat aaatgatatt
     3841 cccatccaac tgttggacga ttagcacaac cacacaacaa agtaatgctt ttaccggatc
     3901 attgaagact gttactagaa atgcaggtgc tagtcctaga taaggtccta aatatggaat
     3961 taggtttgtc aatcccgcaa tgaccccaaa taagaaagca taatccacac caattaccat
     4021 atatcctaaa aatgtgaagg taccaacgaa taaacattcg attgcttgtc cgctaatgta
     4081 attagccaat gttttattca gttgccccaa taactccaca atttggtttc ttcttttttc
     4141 tgggaaaaac tgttggatgt ttggcactag tttttctccg tctttcaaca tatagaacaa
     4201 gatgaatggt gctgtaatga ttactacggt tgccgaagcg atcgttccaa cgatcgaacc
     4261 taaactattg gatactccac taaggaattg ctgaatgatt gtcccgtaag aaatgtctaa
     4321 ttgctcaaaa tatttattga tatctacttc cttgaacaaa ggcaattcgg ctacctcttt
     4381 taaccagctt tctacctgtt tgatgaaatc aggcatatta gatgccaatg aggaaatttg
     4441 ctgtactaga cttgggatga cactaagtaa aatccaaata attgctgcaa ctaaaaggag
     4501 cagcacaatt gcgacagccc atattctttt gatttttgct ttcatcaaca gcttgacaat
     4561 aggattcaat aaatagtaaa gaaagcctgc aatcagtaca ggtgcaaata acgtggaaaa
     4621 aaatgtcccg atcggttcga aaataaagtt tatttctgat gaaatcatga tcaatgcagc
     4681 tatgatcaat aattcaagcg accaaaacat tagtttagac tgtctaagtt tttcaaacaa
     4741 tagatttcct cctttctttc ttactagtat atattatatt cgttaaaaat tcttttattc
     4801 tttttttatt tttgaaaact tgccatactt tttatttgtt ttattttcca gtatactata
     4861 cgaaaggaag gtggctagac catgaaagtt gtacaaacaa aagatacaat gagcaatatt
     4921 tacttagatg ctgtacggat caggcatcaa gtttttgtag tcgaacaggg tgtgcctttg
     4981 tctcgagaaa tagacaaaga tgaagcccat tgtatccact tcgtcctgta ctcagataaa
     5041 aaagaaccgc aaggaactgt acgtcttttg cctttagaaa acggaaaaat gaaattacag
     5101 cggatggcga ttctttctga atacagacac caagggttag gaaaaatatt gattgaagaa
     5161 gcagaaaact ttgctaagaa tcaaggctat aatacgattt tattaggggc acagtccaca
     5221 gctgaaactt tttacgaaaa actaggatat acagcttacg gcgatccttt tgaagatgct
     5281 ggcatgcctc atatttatat gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa
     5341 aacagcaagc ttttgagaaa aatctctcac tggcttactg ttcttttttg tgtttactta
     5401 cgcgacaatt gcttgtaacg gttataccaa atatcaatat agccttccga gaacgggcca
     5461 tctccattat tgatccagtc aaccagaatc ttcacatttt cttttaggat gaagtcaata
     5521 tcgttcgggt aattcatcat tttgctgtgc atctcatatt catcgacatc caatagtcgt
     5581 ttctcgccgt caggaaatac cttgatatcc aagtcatagt cgatatattt caaggcctca
     5641 tcgtccagca aaaaaggtga agctagatta caataatagg aaaccccctt ttctctgatc
     5701 attgctatta cattgaacca gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt
     5761 acccatcggc gtccatcaga ttcagtgact aatgtgtgat cattcacgcc gatcatagag
     5821 tgttcgcttg tttttaatac catggtatct cgccacgttc gatgcaattt gccgtcgtgc
     5881 ttgtaacttt ggatcgtcac aaactctcct tcttttggaa ctcccatagc aacccttcct
     5941 taagcagcat tttgtgcttt gtgtttgggt gttcattaca ccgacatcat aattattata
     6001 tcaataaaaa tgagctttgc ctagaaagga ttcctattgt ttcgattttt ctctaactga
     6061 tctaccagtt tcatttggac cttaggaaaa acgaccgaat caaatgcatc aggcttcaac
     6121 cacttcgaag ttttgttctc ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa
     6181 aaaaggagaa catgccattt cagatgacta aaaatatgag taacttctcc taaatgtcgt
     6241 gtctgccaaa caaagagttc ctgcttttca aatgggagat tttgctcttc ttctgctact
     6301 aggtcatcaa acagatcgat ttctggcttt gtttcccatt cttttttcaa tcgctcatac
     6361 tcctcttgtg tcacttccat cattggaaat gtccacatat tcgccagcaa tttttggcta
     6421 tctctttctt caaaataata cgctccagaa tgattttgaa gagctgcact gatataataa
     6481 acgtcttttg gctttgcttt ttttgttttt acaggaaaac tcgtttggat tcctcgtttg
     6541 ttagctaaac aaaaggcttg gatcggacaa gtctcacact ttggagaagt tggtgtacag
     6601 atagctgaac ctagatccat cattgcttgg ttgaattcac ctggatgctt ttcatcaatg
     6661 attttccgca tcgcttcatc aaagattttc ctgctagaag cttttgcgat gtctgcttca
     6721 atgcaaaaca atctgctgac tactcgcatc acgttgccgt caactgcagg ttctggaagt
     6781 ccaaatgcga tacttgcaat cgcgcctgtc gtatatggtc ctattccctt caatgagcta
     6841 atttcttcag gcgtttgcgg catttcccca tcaaattctg acatgatctg tttagcagca
     6901 gcttggatgt tccgggctct tgagtaataa ccaaggccct cccatgcttt taaaagcttt
     6961 tcttctggag catttgctag ctcttcaatt gtaggaaacc attccatgaa acgataaaaa
     7021 taatcgatca ctgtgtctac tctggtttgc tgaagcatga tctcagagat ccagatacga
     7081 taaggatcac gattatagcg ccaaggcagg tttctttttt cttgttcata ccattgaatg
     7141 aactgatctt gaaattcttt tgtttcttta tctgtccatt tttcccattc tttattcact
     7201 ttcctcttcc ttttccgtaa tgaatcgctg gatcatatcc aaatcaaatc ctttttgata
     7261 taagctttgc tttactttta gatttctttt tttaggttca aaccttaaat ttcttcgcca
     7321 tagtttatct ccttgtttga ctaaataatc atattctatt tcttgatctg cttcttgcgg
     7381 aagatcagct atcgcttgtt ggatcacttc ttgagaaaag cctttcgtca tcaatgtttg
     7441 ttggattttt ctcaataatt ctttttggct ttttccgtga ttttttccaa acgctttttc
     7501 ggctgtctga tgtgcatttt gaacttggtc ttcaaatgta tattggctga gcgcttgttc
     7561 aatgatttca ggtttcaaac ctttttgctg catctgctgc gctaatttct ttggtccttt
     7621 gtcacttaag cgcatattcg tgcgcacata actttcccca tagaccaaat catcgacaag
     7681 gtccaattct tttaagcggg ctacgatttt atttcgatct tcttgaagga tttccttttc
     7741 ttttaagtat gttcggattt ctttttccga tcgcaactga taacttaagt aattcattgc
     7801 taggtgaaaa ccgaaatcag ctccgctact cttcttgatc tcttggatag tttcttcgtc
     7861 taattcccgt ccttttaata atcgatagcg tactaataca tcttcagata cttgtaaagc
     7921 ttctccttca gataaatcta cgcgatagaa agggcctttg cctttactga ttctcgtaac
     7981 ggttaacata aacatcctcc tcgaacatat attctctatc ataccacaga aaatccccgt
     8041 aatcaggtat tatatgttag aataaaccac agaaaaaaga aaaagggaga tacttatggc
     8101 agaaacaatt gtcaaaactg gacaaacggt tcctttgaaa atcaagcgtt taggcataaa
     8161 tggtgaagga atcggttatt tcaaacggtt gatcatattt gttccatatg cactaccaaa
     8221 agaagaagta ctagtgaaaa tcacgaaagc aactcctcgc tatgcagaag gagaattaat
     8281 aaaagtcaaa aaaacaagca aagatcgcgt agtggcacca tgtcctgttt actatgaatg
     8341 cggtggctgt caattacaac acttagctta tcaagcgcaa ttggatttta aaaaggatct
     8401 gcttttgcaa gcattggaaa aattcaaacc agcaggcttt cgatcttatc aacttttgcc
     8461 gacgatcggg atggatgagc cttggcatta tagaaacaaa gcacaatttc agttacgaaa
     8521 aaataagcag actcaaaaaa ttgaagctgg tttatacgaa gcaaactctc atcagttagt
     8581 ccctataaca gactgcttgg ttcaagaacc tcgaacacaa aaagtaatga atactgttgt
     8641 tcaactattg aataaatttg atgcacccat ctatgatgaa cggacaaaca gtggaatctt
     8701 ccgcacaatc atggttagaa tcggtgttaa aactggggag ttacaagttg tttttattac
     8761 tcgcagtaaa aaattcccgc aaaaaaacgc aatgatccgt gagatcaatg aacgactgcc
     8821 cgaagttgtt tcaatcatgc aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga
     8881 tgaaacgatc cacttatggg gaaaagaagc cattgaagaa caaatcaatg aagtgacttt
     8941 cgatctttct cctcgtgcct tctttcaact caatcctcag cagaccgaat tgctttatgg
     9001 cgaaggaatc aaagcattag atgtccagcc taatgaaacg atcgtggatg cttattgcgg
     9061 agttgggacg atcggcttaa gtgtagcaaa acaagccaaa gaagtacggg gaatggatac
     9121 cgttcctcaa gcaatcgctg atgcaaaaaa aaatgcggaa agattaggat atcacaacac
     9181 tcgatatgaa gtcgggacag cagagcaagt tctgcctaaa tggttgaatg aaggttttca
     9241 gcctgatggt atcatcgtag atccaccacg tacgggtttg gataacgcat tgatccaatc
     9301 attattgaaa taccctccaa aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc
     9361 cagagatctg gttaatttaa gtaaaaagtt caagattgaa tatttacagt ccgtcgacat
     9421 gtttcctcag actgcacgtt gtgaagttgt cgtgaagatg acgagactcg ctgactaaat
     9481 gaaagaggtt gtgaaaaagt ctgccctgta tcaagtaata agaacagcca aacaaaaata
     9541 gctcttcatg ttttatgaat actgtacttg tgaccgaaga gttaacagga ggacactatt
     9601 tatttaaaaa aagggttgtg acatagtttt tgtcacagcc ctttttgatt attattggat
     9661 attcacgtct atcacttgat aaaatgcatt tgtcgtatcg ctgatattcc atactgctaa
     9721 aatcacatta tatccttttc gatcctgcgg aagtgtgatc gtttggttga ctgtttttgg
     9781 tggaacagtt gctttgtcgt ctatttttgt gatcaattca aaatcactaa atttcaaagg
     9841 ttgattagga ttccaagttg gtttggtaat gtagtaatcc caagtacttg tccgatgttg
     9901 tgctgttaaa ttccaagtaa tatttaatgt tcctggatta accatgtttt tatgccatct
     9961 gttagatgtc tgttcatcta aaggctcaaa gccgctgact cctgcactag caatttttcc
    10021 atcaatgaat gtatttttgg tagcttcgat actttgcggt tcccattgtg ctcttccgac
    10081 attttgattt aagtttcctg ctgctgatgt tcctaggtat gcacggcttc ctggagaagt
    10141 aatataacca tgcgcaaaac ctggaatgct aaaaccgaac aaccctaaac taactaaccc
    10201 aataaacaat aacgtactaa tctttttcat gtagaaacct ccttcatttg ataaccacat
    10261 tgtaaacgct ttattgtatt aacacaaaca ttatgctttt ataattttat gttgaagtaa
    10321 aaaaggtgaa aaaagtaaga tgcagattat aacagaagta gacgagaaaa gtacaaatag
    10381 aaaacatgag aatcaaatca tatgatcttt ttcaaaaaca tcattcatat aaacaaatgt
    10441 tcaacttttt aataacaaca attatcaatt tttttcttac ttcacaaact aaatacaggt
    10501 cttgctgtga gccttcatgg taaactacta ttatagaaag gagttagtaa aatgaagatg
    10561 ataaaagata ttcgtttttg ggtttgtgtt ataggaattg tcatattagg ctttttatca
    10621 gggttgctta gcggaaatcc cggagaatat tactattctt tgcaacttcc accatttgca
    10681 ccacctagct ggatatttgg gccgatgtgg accttactgt atatattgat gggtatttct
    10741 ctttatttgt tacttaatca caacaacaaa aaacagcgaa acaatcttgt cggtcttttt
    10801 gtgattcagt tcatttttaa ttttatatgg tctgctctat tttttaattt aagaaatatt
    10861 ttcattgcag caatcgatat tacactttta gttattttct tgtcagtgct tatgtatcag
    10921 ttgtggctac atcatcgcct ggcaatgtgg ctcatgattc cttattattt atgggtacta
    10981 ttcgcaactc tattgaatta ttcaatttat tttttaaact aagcgctaaa aagagacgtc
    11041 ctgtaattta acacatagaa aaagtgccac ctaagttaga ttttttgtct aatttaggtg
    11101 gcactcttta ttaaccaaac tatttttttc tagtacccac ttgttgttcc atactcatta
    11161 ttctggtcat aaccataacc attttgttcc tgtccagcgt aaggatcata actttgagta
    11221 tcttgacttg gtgtatagta ctgacttgta tcttctgttt gctgataata ggtattcgga
    11281 tcttggtact gatcatatgt gttttctgaa taggaagaat aatcggtgta cccttcacta
    11341 ttcgatccat caccgtttgt cgaatcgcca tcttccgaat catcaaagaa catcattgaa
    11401 gcattctcat ttttcaaatt aggtaatgta tcgcttgttt ttatatttaa ttgtttcttc
    11461 agttcatttt ggatcgaaag aagatcattt tttcctaaaa tttgatagta gatatcattg
    11521 atcatttgtc cttcgccttg caactggtct tgtttgattg tttcaaacgc aggggtgtag
    11581 ttcgtcgcta catcaagcat gtcatcccaa tctaagtccg tcgtcacatt gttctcaact
    11641 gcttttaaaa tttttctata attgcttacg ccatctaaac ttaaaacttt attgacgatt
    11701 ttcgtcacta cttcccgctg acgacgttgt cgaccgatat cgccttctgg atcttcgtgt
    11761 cgcattcgag aataggctaa acctttttct ccattcaatg tttgttcacc tactggtaca
    11821 tggacaccat ccaaagtaaa gtcaatttta ttattcactt tgatgccacc gaccgcattg
    11881 atcaaatctt ccatgccatc caaattgatc gttacataat gatcaatagg aatatcgagc
    11941 agttgctcaa ttgaatccat ggccatttcg acaccaccaa atgcataggc atgatttaat
    12001 ttatctaccg ttccataacc tacgatattc gtataaatat ctcggtccaa actgatgatc
    12061 gttgattttt tttgtttcgg attaactgtt actaccatca tcgtatcaga acgtccttgt
    12121 tccgttcttc ctaatccacc ggtatctaaa cctaatagca aaaccgaaaa aggttctttg
    12181 tcatcaatgc ttactctctc agaacgtttg gaggatattc gattagagtt tttagaaatt
    12241 ttgtttactg tttggctcgc ttctcccata aatctgattc cgtatgctga taatccggca
    12301 atcagcaaaa caaatacccc taacaaggtt aatagaactt tttgccatcg cttcataata
    12361 cacctctgtt tcacattttc taaaatattg tctcatataa ctgttcaatc agcaaattca
    12421 ctttttaatc ataagaaaac tttaaaaaac atttttgtgt aacgattact attttttaaa
    12481 atttctcaaa attcttaact atcataccat accgtgaaaa agatcgaaag accgaataca
    12541 cgcagttcgc aatcgtttta tcttttttta cgtatttttt ccagaaaaag cgaacaacaa
    12601 ggtattttcc aaataatcgt gcagtaaaat gatcgcacat acctttccca tctttttaac
    12661 agtctttgcc taccgttcag attatcattc gatttcacga aaatttcttc ctatgactgg
    12721 tccttttgtt aaacccatct tctattcgtt ttatttccag ctcactgcat ggctcttaaa
    12781 atgactcgaa ctagtgattt cattaccatc atcttttaat ggatagatat gctcctcttc
    12841 aaagtaaaac gattttcttt taaagtaata gaattgacat ggtaatgagt atgtatacta
    12901 gtggtttcac aaatggtctt ttattcaaca tccaaaaact gaaatttctt taaaatccat
    12961 tactaacgaa gaaaatgttc cttgcttgat aattagttag gacatgtcaa gtgaacaaat
    13021 gcatttactt agccaaaaaa tctaaaaact tcataaatag acaaaaagac acgactctct
    13081 aagaagtcgt gtctttattc aacaaattat tataatttag tcacttcagc agcttgaggt
    13141 ccgcgtgcac cttcaacaat cgtgaattct acttcttgac cttcttctag gctcttgaag
    13201 ccgtctcctt gaattgctga gaaatgaaca aatacgtcat ctccgccgtc tactgtaata
    13261 aacccaaatc ctttttcatt gttgaaccat ttaaccgttc cgtgttccat aaatgtatag
    13321 cctccaaaaa agttcttgca agtaaccttg caagaacatt atacccaatt tagaaaacgc
    13381 tagcaagctt tcgctctttt attttccaaa ttatttttaa gactgatatg acggaggaaa
    13441 tggttatttt gctgcttcga aacgtttgtt tacttcatcc caattaataa cattccagaa
    13501 tgctgcaata taatctggtc ggacattttt atatttcaaa taataagcat gttcccaaac
    13561 gtccaaacct agaattggtg tctttccttc catcaatgga gaatcttgat ttgcagtaga
    13621 ggtaatagct aattttccat tttccattac aagccaagcc catccagaac caaatcgtcc
    13681 agcggctgct tttttgaatt cttctttaaa agaagaaaaa tcaccaaaag cttcattaat
    13741 cgcttctttt atttctcctg taggttcgcc accagcattt ggtgccatga tttcccagaa
    13801 aaatgaatgg ttagcatgtc cgccaccatt gttacgtaca gctgtcttga tatctgttgg
    13861 aatagcgtcc atatcagata ataattcttc tattgttttt tcgcctaatt ctgggtattt
    13921 ctcaattgct gcatttaaat tcgtcacata agtattgtga tgcttatcat gatgcagatg
    13981 catcgtttct tcgtcaatat aaggttccaa tgcgtcatat gcataaggta gatctggtaa
    14041 tgtgtaagtc ataaaaaaat tcctcctcaa tgttttcttt ataatgtcta cattacaaag
    14101 agtaacaaag agaaggaaat tcttcaattg ttatgcctag tcgtcttttt cactttgacc
    14161 tacttcttct tcaaaaacct cagtttcttt aaaaataaac aacttgctga aaatatagtt
    14221 cgctaggata acaataattt gagtgatgat tttcgccaca tactcattgc tgttcataac
    14281 attgatcaac aataccattg cccccatgtc caatccgtaa gaaaggatac ggtagaagaa
    14341 gaacttaccg cattcaataa gtgcatgtgt aaaattcggt gattttgaat ggaataccca
    14401 aagtttgttc gttacaaaag caaacaatac agaacaaaac cacgaaatag aattggataa
    14461 ggccatactt aaatcaaaat attggcgtgc gagagcaaaa ctaacaaaat ttacaatcgt
    14521 agccaaacca ccaaaaaaga gatagactag tatctcccaa tatccttttg cttctaaata
    14581 tccttttaac tgtcgatact gcttcatgag ccaacctacc ttattaaaaa ttcttattta
    14641 tcatactcta tatttcaata actttcatct ttatttaaaa aaattaattt tttttgatcc
    14701 tttgcctttg tctctgcttg gatcgtcaca tgtgtaatac catattttaa ttttaataaa
    14761 gaaattatac gaggatatat cttttcaatc tcgcttagta gctgatcttc aaaatcaata
    14821 tgtccgtcga aaataattat cttttcgcta tactgccaaa tatggacatg atgaacattc
    14881 tttaccccat cgattgcttc aatttcttgt tccatttttt catagtcaat agcaggtgca
    14941 ctttgtatca aaatagcggc agcttttttg atcaccatta tcgcttctat caatatatat
    15001 atcgaaatga tcagtgtaat aactggatcg atccagtaga tatcgaaaaa acggatcaaa
    15061 attgctccga taatcacact taccgaagac agtgtatcac ttaacaaatg caaataacta
    15121 gattttatat tcaaactttc atctgcttct tgtcgtaata ataatactga gaacaaattc
    15181 gcgagcaatc caataactgc tactgttagc atcaaatgac tatttatctt ttctggtgaa
    15241 gtgaaccgct tgaatgcttc aacggttaaa aacaaggata atatgatcaa tatgaatgag
    15301 ttgatgaaag cactcaatat ttctaaacgc tggtagccat acgtacgttt tttatttgct
    15361 tcttttcggg ccattttttg tgccccgtaa gaaaaaataa ttgccaatgt atcactaagg
    15421 ttatggaaag catctgaaac aagagacaaa ctccctgaca taatccctcc aataaattct
    15481 gataaagtga tcaccgcatt caaaaccact actccaaata gtttttttga tgatctctgt
    15541 ggctgtgtag gcactattcc ttcctccatt ttatttatat tggatagtct ttagacaaga
    15601 ataacatttt tttgatattt caccaatcat tcagatgttt ccctaaagca cttgtatttc
    15661 acttgatact tcgctagaat tgcaagtgat gacactacta gatgaggagt acttatgaca
    15721 acaaaacaat taattatcag ttcctttata cgattcaaag acctaaagga gacgagaaat
    15781 gtcccatttt ggaaagccat tgtctatctt ttagttttga gtataattat ggctttgcca
    15841 atctcttttc aagtttttca agtcttaaac aacattcaat tggatgggca aaagattgct
    15901 actcgcattc ctgattttac aatccaaaac gggcagcttc aaaccgaaga aaaagaagga
    15961 ttcatttacc aaacaaactc cattatcttc acatttgatc cagagggaaa acgaacagaa
    16021 caggacatct ctactgattt gatggggaat tttgttagtg tcgggatgtt aaaaaacaaa
    16081 cttatcatag ctcttcctaa tacaggcacg acttctgctt tgttgaacaa taaccaatta
    16141 gaacttccct acaccaatga atctttaaaa aacttaacag gtaaggagct ccgttctttt
    16201 ttaagtaaag catctatacc actttggata aaagtcctga catttatatt ttctatttat
    16261 cctagttttc tgaatctgct gatcactttg ctatttacca acattgctgc ttttctctat
    16321 gcacgctttc gactaacaaa agctactttt cttgattgtt taaaaacatt gatctattca
    16381 atttcattgc ctactataat tgcaacaatc ttgatggtct ttcttccttc ttttgacaca
    16441 agcgccttca ttgcttttgc aggaatcttt atttttgctc aagctgtcaa aggatgggca
    16501 aaaatatcga tcaattaaga aataaagaca actcgttatt tctacttcaa tagacccttt
    16561 tagaggttgt gacatttttt tgtcacagcc ccttttttat ttgaaaaata tttctttctc
    16621 aaaaaacaag caaggcgctt caaagttctt cttgtatgcg ctacacttag agtaaagaaa
    16681 ggcgggaaat taactatgtc agaaagaatc atttatgcag atcgtccatt tcgtgatgaa
    16741 tttatcgaag aaatccatac tatagcgcct ggttatatct ttaaaacaga attacaacca
    16801 gaggatctac aaaatgtaga gatcagtttg ggctggaact cagactacca agaaaaacta
    16861 ttagcctctt ctaccttgaa atgggtccaa tctatctcag ctggtgttga tcagctaccc
    16921 cttgagactt tttcaaagaa acatatttta ctttcaaatg gtagtggcat ccacagtgaa
    16981 tcgattacgg agcatgtctt aggaatcatc cttgggtatt ctcgtggtct ttttcaagca
    17041 caaaaagcac agagtgacat gaaatggtta ggttcatcgg tccattacca atctatccaa
    17101 ggaaaaaaaa tactaatcat tggaactggc catattggaa aaatgattgc aaaaaaatgc
    17161 aaagcattag acatgaactg ctatggaatc aatacatccg gtcatccagc agaaggaatg
    17221 gaaaagactt tttctttaaa tcaactaaag gaagttcttc ctgaagtaga tttcgtagtc
    17281 aatatcttac ctctaacgga ggaaacaact gggctctttg atcagcatat gtttgaacac
    17341 tttgatccaa aatctgtatt catcaatgtt ggccgtggtg cttctgttaa gacacaagat
    17401 ctggtgcagg ctttaaataa ccagcaactt tcctttgcag cattagacgt ttttgaggaa
    17461 gagcctcttc cacaagatca tcctctttgg aaaatggaca atgtactgat tacttcccac
    17521 attgcaggtt tgacacctga ttttcaaaag aaattaatgg ctattttttt aaccaatctg
    17581 aaaagctact ttgccaccca tgagcttcaa accaatcaag taaaattgtc tgcaggatat
    17641 taatagctga aaacaatatt ttcaaaagaa caaagagaag gtgacaaa
//
LOCUS       DACEQH010000054        17630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.055, whole genome shotgun sequence.
ACCESSION   DACEQH010000054 DACEQH010000000
VERSION     DACEQH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.055"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            288..695
                     /locus_tag="IW833_002041"
     CDS             288..695
                     /locus_tag="IW833_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308141.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="IW833_002042"
     CDS             709..1110
                     /locus_tag="IW833_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308142.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="IW833_002043"
     CDS             1112..1483
                     /locus_tag="IW833_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308143.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1519..1821)
                     /locus_tag="IW833_002044"
     CDS             complement(1519..1821)
                     /locus_tag="IW833_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308144.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2070..2270)
                     /locus_tag="IW833_002045"
     CDS             complement(2070..2270)
                     /locus_tag="IW833_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1308145.1"
                     /translation="MKINYTVDLVSFSLVINFCLFSLRLFIEGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(2575..3807)
                     /locus_tag="IW833_002046"
     CDS             complement(2575..3807)
                     /locus_tag="IW833_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1308146.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSAAGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVIDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            4064..4633
                     /locus_tag="IW833_002047"
     CDS             4064..4633
                     /locus_tag="IW833_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1308147.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4811..5251)
                     /locus_tag="IW833_002048"
     CDS             complement(4811..5251)
                     /locus_tag="IW833_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ1308148.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5409..6173
                     /gene="map"
                     /locus_tag="IW833_002049"
     CDS             5409..6173
                     /gene="map"
                     /locus_tag="IW833_002049"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ1308149.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            6205..7128
                     /locus_tag="IW833_002050"
     CDS             6205..7128
                     /locus_tag="IW833_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ1308150.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            7204..8343
                     /locus_tag="IW833_002051"
     CDS             7204..8343
                     /locus_tag="IW833_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ1308151.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            8336..9136
                     /locus_tag="IW833_002052"
     CDS             8336..9136
                     /locus_tag="IW833_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1308152.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            9136..9963
                     /locus_tag="IW833_002053"
     CDS             9136..9963
                     /locus_tag="IW833_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1308153.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9941..10675
                     /locus_tag="IW833_002054"
     CDS             9941..10675
                     /locus_tag="IW833_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1308154.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            10775..11641
                     /gene="rfbA"
                     /locus_tag="IW833_002055"
     CDS             10775..11641
                     /gene="rfbA"
                     /locus_tag="IW833_002055"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ1308155.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11655..12227
                     /gene="rfbC"
                     /locus_tag="IW833_002056"
     CDS             11655..12227
                     /gene="rfbC"
                     /locus_tag="IW833_002056"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ1308156.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            12249..13277
                     /gene="rfbB"
                     /locus_tag="IW833_002057"
     CDS             12249..13277
                     /gene="rfbB"
                     /locus_tag="IW833_002057"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ1308157.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            13375..14226
                     /gene="rfbD"
                     /locus_tag="IW833_002058"
     CDS             13375..14226
                     /gene="rfbD"
                     /locus_tag="IW833_002058"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ1308158.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            14260..16293
                     /locus_tag="IW833_002059"
     CDS             14260..16293
                     /locus_tag="IW833_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308159.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            16337..17617
                     /locus_tag="IW833_002060"
     CDS             16337..17617
                     /locus_tag="IW833_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ1308160.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYGEQPDSAHSSRIQRYH"
BASE COUNT         5531 a         2966 c         3590 g         5543 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtccggcacg tcagtcacga tatcaaacaa
     1621 aaagtataca cccttcttca ttctagtttt cgcagcagga attttgaaat agttcttatt
     1681 agaatagtag agattgatta ataagctatc ttcgattgct aaaaagaaaa cttctgaatt
     1741 ccatacttta taaaaatctt tgataaatct attcgaaggg tcaaatttaa accataattg
     1801 tgtttttcct tccatcagca taaaagttca cctcaaaaag agtatacgaa cgaatgttct
     1861 ttttgtgaag gcggaattta atatactgaa taatagaaaa caagtacttg tgccaaatta
     1921 tgtgccaaaa aaattcgaat tcaacaaaat gtaaacaaat agaaaacctt ctatattaag
     1981 atttcctgca ataacaaaca caacagaaga cgtgtaatag ttagtcagga acgtacaaat
     2041 aacccctgta tcctttgcgg tacagggggt tattttgtat aagtagacat tgtagtttct
     2101 aaactgctat gtcctaaacg tttcgatacg ctaagtatat ttactccttg ataaagtaaa
     2161 atagatgcat gcgtatgtct tagtccctca ataaacagtc gaagagaaaa taggcaaaaa
     2221 ttgattacta aagaaaaact tactaggtcg acggtgtagt tgattttcat acttcaacag
     2281 attcgaatta aacgatttcg attggcttat accagtccta tccgacgaaa gatatatcgt
     2341 tcttaactct agcggtataa cttgaagctt tacgtaaagc caatgggaat gaactggaga
     2401 tgacttttta ttatttttag taatagctga tgggttaata cttacaactt tccttttcaa
     2461 agtaccatat aaaaaaacgg tggggttgtt tagcgaaata aaggatctaa gccaatagct
     2521 tagatccttt attctatgct ttatattatt ttttgcatgt gtgcgaaagt atactcaagc
     2581 ccaatcgcca tttcggaaaa tcggtactgt agagccatct tcacggattc cgtcgatatc
     2641 catcttgtca gatcctacca tgaaatctac gtgggtttga cttctattca atccagcttc
     2701 tgctaattct tcatcggaca tttctgtacc gcctttcaca ctgaaggcat aagcagaacc
     2761 tagtgctaag tgattcgatg cgttttcatc aaataatgta ttgaaaaaga tgatgccaga
     2821 ttgagaaata ggtgaagggt caggtacaag tgcgacttct cctaaacgac gcgcaccttc
     2881 atctgtatca agcagttttg ctaagacatc ttctccttgt tcggcagaaa aatcaatgac
     2941 ttttccatct ttaaaagtaa atttcatacc cgagatgatg gtgccagcat aactcagtgg
     3001 ctttgtactt gaaatgtaac catctactcg atgactgtca ggtgctgtga acacttcttc
     3061 cgtcggcata tttgccatga atttttctcc acgagcatta tagcttccag cgccttccca
     3121 aagatggttt ttaggcaatc cgataatgat atctgtaccg ggagcagtat agtgcagggc
     3181 agaaaattgt tctcgattca attcttctgc tttttttgct aatttttcat catgcttctt
     3241 ccatgctaag acagggtctt cttcatagac acgagtcgtt ttgaaaatct gatcccataa
     3301 tgcctcaact tgtttttctt ctggcagttc tgggaagact tttgctgccc attgtttacc
     3361 agccgcagcc acgacagtcc aactgacttt atttgcttga gttgccttgc gtaaattcat
     3421 tagtgctttg ccagccgctg attgataaga cgctactcga tggctatcta caccagcaaa
     3481 tgcatcggga tcggcagaaa caacactgat cctgcttgct cctttttcaa gccattcatc
     3541 cgcttgatcg attttagatt gtggtacatt ctcaatacga tccgttgcag catgtaaaag
     3601 aaattctctt tggatctggt cgtcagtcca ttgaacgata acctcagcgg ctcctagttt
     3661 ataagcttct tgtgtgatca atcgagcaag gggtgcctgt tccacgctga tctgtaaaac
     3721 aactgtgtgt cctttttctg tggctacgcc agtttctgca attaagcgtg catatttttt
     3781 tagaagttca ttaaaatcag ataacataat aaaaattcct cctcaccatt ttttctggta
     3841 actattagca tgataccatt taattatgat tcaaacaatg aggcctttaa ataaaaaaaa
     3901 gaggttattt ttttaaggtt aagtttcatg atatatgtta ctacttgcta tttcagcttg
     3961 tgaagaaaat caaaaactaa ttttgcatat caaaaaaaac atgttacaat gaaattaatc
     4021 aatggtggaa gcgttataaa aaaattaagg agattaaaaa aatatggcaa gaaaaaagac
     4081 gattactaga gaacagattt tggcagctgc ttatgaagta gctgccaccg atggatttac
     4141 acgatttact gcgagaaaca tcgcgaacaa aatgaattgt tccacgcaac cgatttatct
     4201 tgagtttaaa aatatggatg acttgagaca agctcttttt gagcagatct atgatcattt
     4261 acaatatgaa gtgttcactg ttgagcatac agggaatacg attatcgatc ttgcttttaa
     4321 ttatatccat tttgcaagaa gagagaagaa gttgtatcgt tcactgtact tagaagaata
     4381 tggtggagga aaagagatgc agaatttctc ttatacttac tttgtgaata cagtgaaacg
     4441 cgatccagct tatgtgaatc tttcagaaga gcagatcgaa tctttgtata atgggacatg
     4501 gatcatagct actgggatcg cagctttgat gtcttcaagc atcatccatc caactgatga
     4561 acagattgaa aagatgatac aagacagtat agatgctatc ttgaaattca aagaaccaat
     4621 agaaatcgat tgaatatact caaaagttcc acaaatcagt agataacaag agatgtgtca
     4681 aaataaggaa aagttcgttt ttcttacagc gtatgcattt ttaactcccg aagaactctt
     4741 ccatgaatgc aatgaataaa agagccgtac agattgaata aatctggatg gctcttttat
     4801 tgcaagtata ttaaattttt gcgactagtt ctttagcgaa agcttctaaa ttttcgatat
     4861 cttcttcttc agcattcaga tccactttaa cattttcggc tccttttgat gcgcctgttt
     4921 ttgcaaaagc agcatcgaaa tcatccaccg atttgcagaa ttcatcgtaa aaagtatcac
     4981 cagatccgca gacaccgtaa atttttcccg atagatccaa ttcttgcaag tcttcataaa
     5041 agtctacgat ctcatcaggc agatcgccat catcgtatgt ataagtagct actacgcaaa
     5101 tatctgcctc ctcgaattca tctgcatcta cttgggtaca ttcgttgatt tcgacttcga
     5161 tatccagatt ttcaaatgct tctgcaacaa tatcggcgat ttcttcagtg tttcctgtca
     5221 tgctagcata tacgattttt gctaaagcca tgatgtgcct cctcgtttat aattatttaa
     5281 caattatgaa ttataccaaa acatcattga aaagaaaata aaacctagag acacctgggg
     5341 aatgtcattt ggctaaatgt atgccgctat gttaaaatga gagggatttg taaaaaggag
     5401 acgaaaatat gattacttta aaatcagcac gtgaaattga aatgatggca gaatctggag
     5461 ctttattagc agatgtacat aaaaatctgc gtgattttat aaaaccagga atcacaagtt
     5521 gggatattga ggtatttgtc agaaactata tcgagagtca tggcggaatt gctgcgcaaa
     5581 ttggctttga agggtatgaa tatgcgactt gtatcagtat aaatgatgaa atttgtcatg
     5641 gattccctcg taaaaagcct ttgaaaaacg gtgatttggt aaaagtcgat atgtgtatcg
     5701 atttgaaagg cggcatttct gattcttgtt gggcctatgt agttggagaa tctacacctg
     5761 agatcgaccg attgatggaa gtaacgaaga aagctttgta catcggtatt gagcaagcac
     5821 aagtagggaa tcggattggt gacatcggtc atgctatcca aacttatgta gaaggcgaaa
     5881 atctagcgat cgttcgtgat tttattggtc atggcgttgg tcctacgatt catgaagaac
     5941 cagcagttcc tcattacgga gaagctggaa aagggttgcg gttgaaagaa ggcatggtta
     6001 tcacgatcga acctatggtt aataccggaa cttggaaaat gaaaatggat cctaacggct
     6061 ggactgcgta tacaagagac ggcggacttt cttgccaata tgaacataca ttggctatta
     6121 ctaaagatgg tccaagaatt ttgacatcac aaggagaaga aggcacttat tgatctatcc
     6181 agtcaatagg tacgggagag gtacatgaaa gtttggaaca aaataaaaca aaatgaaaat
     6241 ctgatgcgct ttattgagac gactcaaaag cgcatcactg attcagaaat gagtacgacc
     6301 tctgtcgtcg ttgcttatta tttactcttg tctttatttc cattgattat tgcttttggt
     6361 aatattttac cgtttctcca catcgatcaa gagactgtac tgacctattt acgagaagtg
     6421 attcctgaga ccgtttacca gtttatcggt cctgcaatca aagacctttt gacacaaagt
     6481 tcaggaggct tgttatccgt ttctgcatta gcggctttat ggtctgcaag tcaaagtatc
     6541 aatgctttgc aaattgcgat gaataaagcg tatggcgtag aaaatagaaa aaattttttg
     6601 atcgtccgtt tcttctcact aattgtcatt gtcttgttca tgattgctat tagtggggta
     6661 actatcgttc ttgggttggg acagctgatt ttggatgcta ttcagccaat cgtacaaata
     6721 cccgtgaact ttatcgatca gttccaaact ttgaagtggc caatcacatt ggtagcttta
     6781 tttgtcatca tgttcttgat ttatttaatt gtccctaatg ctcagttgaa gttgaagacg
     6841 atcattccgg gcacgatctt cgctactgtc ggctggatgc tgttatcaca agtgtttagt
     6901 atctatgcta ggtattttgc aacgaggatc agcggttatc agatcatagg aagttttatt
     6961 gtactcatgc tttggcttaa ttttgctgct acgatcatca ttttgggcgg aatcatcaat
     7021 gcagttgttc aagaatacgt gacgggtcat caaatcaaaa aacgtcgaga tccaagccga
     7081 aaatggttca ataaattgaa aactaaattt tctcataaaa aaaagtagaa acaagagttc
     7141 ttattgtaaa tttatatcag taatttataa taattatgac tcttaattaa agtaggtgcc
     7201 catatgcttg tttcttttag tatggttata agattttttc tgacaatttt attatcactg
     7261 atattaacac ctgtattcaa aatcatttca gtacaaacag gtatggtcga caaaccaaat
     7321 gaaagaagaa taaataaagt tccaatgcct tcagcgggcg gactgcctat ctttatttca
     7381 tttgttatat cgactttatt cttattccgt aaaattatcc ctcaatcgta tattgtaccg
     7441 atcttgattg cttctttagt aatcatagtc acaggagtgc tcgatgataa atatgagctg
     7501 acacctaaac aaaaaagtat agggatcttg attgcttctc ttattattta ttttgtagca
     7561 gatatacgta ttgattcctt cacgcttcct tttattggat atatccaact aggctggctt
     7621 agttttcccg taacgatttt ttggatcttc ggtatcacga atgcggtcaa tctgattgac
     7681 ggtttggatg ggctggcggc agggatttca ttgatcggtt taattacgat aggaatcatt
     7741 ggatattttt tcctccatgc ttctacagtg tatattccag ttgttatttt ctgtcttgta
     7801 gcaagtatta tcggtttttt tccttacaat ttttatccgg caaagattta tttaggcgat
     7861 acaggagctc tattcctagg gtttatgatg gccgtccttt cactacaagg gctgaagaac
     7921 gtgacttttg tttcatcgat ttctttgctg atcgtcatgg gagtgccggt aacagacacg
     7981 ttttttgcta tcattaggag aaaagcaaac cgtgtttctt tttctacagc agataaaaaa
     8041 catcttcatc accgactaat cagtttaggc tttactcata aaggtgctgt cctgacgatt
     8101 tatgcgatgg ctttgatgtt ttctttcaca gctatggtca tgaattatac tggaagaatc
     8161 ggaacgatca tattgattat tgccatgttg tttgctgcaa ttttattatt tgaattgatt
     8221 ggtctgatca atgagaaaca tcagtttatt ttaaaaacat tgcgattttt agggaataaa
     8281 gagtaccgta cgcaagtaaa acagaaatat ttgaagaaat ggaaccgtca tatagatgaa
     8341 taacaaatta aaccagccga tccttattag tattgtgacg gcaaacagtg ataaaatttt
     8401 tcggacacta gataatttta tttcaactgt aaaagatgaa acagcagtaa aaatccgaat
     8461 ttttgacaac aattcacttc ccgagtacaa aaaaagacta acagagtata ggaaatatcc
     8521 ctacatcgat atctattttc atgatgaaaa ttccggattt ggttatggac ataatcacaa
     8581 tctgatagct agcgattctc gctatggcat tatttgtaac ccagatattt tagtggatga
     8641 agagacagtt acagcattgg tcttgttatt gaaagaacat ccggaatgtg cgatgttagc
     8701 acctaagatt cttaatgaag atggaacaac tcagcacctc atccgtgagc agctggctgt
     8761 ttttgattac gttttgcgct ttgtgccttt caaatgggtc aaaaaaaagt ttgacaaacg
     8821 actggctgtg tttgaatgcc gaggattgcc tactgatgct aatagttatg tacggatgat
     8881 ctcaggtagt tttatggtag ctgatattga taaatttaaa gagataaagg ggtttgatga
     8941 gcgtttcttc atgtactttg aagacaacga cttgtgtttg agttttgaaa aagcaggtta
     9001 caagttattg tacacaccct tttatgcagt tatccatttt tacgggaagg gtgcgcatcg
     9061 aaatgcaaaa ctgtttcgcg tgtttttgac ttctatgatg aaatttttca ataagtgggg
     9121 gtggcgtttt ttctgatgaa accagaaata ttagtagtcg ttgtgcttta tcagcaacta
     9181 ttcagtcaat ctccagctta tggaccatta agcaaagcac ttagcgaaaa gcaaattcag
     9241 cttattattt acgataatag tcctcaaagg cagcaaaatg agctgtttga aaaagaaaat
     9301 atcgtttatc atcatgatcc gcaaaatcct ggtttagcgg tggcttataa ttttgcgttg
     9361 tctcaagcag atgaaggaac acgttgtctc gtcactctgg atcaggatac gaggctagtc
     9421 gacgattatt ttgaactatt gcgtaaagta acctttacag atgaatgtgt tgctgcagtt
     9481 ccgatgattt tttctgggag ccgacaagtt tctcccctat attcagatcg ttatatcaat
     9541 cgtacagctc aagcagtaga agttgaaggc ccaacagctg aacgaatcat ggcaatcaat
     9601 tcaggtgttg cctggtctgt caaatttcta aaaaagattg gcgggtttaa tcctgctttt
     9661 tctttagatt ttttggatca ttggctgttt tggaaagtgg atcagttgga aaaaactgtg
     9721 gagatccttc ctgtacgaat ggaacatgat ctttcggttt tagactatga aaaagtttca
     9781 agtaatcgat accgttctat tttacaagca gaaaatcgat tttatcatga atatgatcaa
     9841 aggcatttat ccagtcaccg acgacaatta ttgttaagaa cagctaaaca gttcttgacc
     9901 gtcaaaaacc ggcatatctg gcggatgact ttgcgctcat atgttaagaa ttggaaggtg
     9961 tgaattcgtg cgttcggttt gtatagctac ctttaatgga ggcagatatt taagagagca
    10021 gttagatagt attttatctc agctttcagc agaagatgaa gtagtcattt ctgatgatgg
    10081 gtcagtagat gatacatggg atatattaac tgaatacgca gaaaaagata agcgaatcca
    10141 gcttttttcg gggcctcgtc aaggattgat cgctaatttt ggttatgcga tccagcagac
    10201 gaaaggagat ctaatctttt tggcagacca agatgatgtt tggttggaga caaaagtgaa
    10261 aaagatcact gactattttg agcagaatcc ggatcagcta gtagtagttt ctgatttaat
    10321 cattgtggac agcgagctgc aagagatcca tgattcgtat ttttcgtttc gaaattccaa
    10381 agaaggatgg cttaataatg tgataagaag tcactatatt ggtgctggta tggctttccg
    10441 gtcttcttta aaacccgata ttttgccgat cccgtcagaa gtgccgatgc atgatatgtg
    10501 gattggtttg ctgggaggaa agaagactgg gttcctaaga gaaccgttaa ctctgtaccg
    10561 aagacatgat ttaaacgcga gtgagattga aacacattct aaattacggc aaaaaatttc
    10621 ttggcgtgtt catctgatta cggcgttgat caaacgaaaa tggtttaaac gttgataaaa
    10681 gatgtttaaa tccttaatat ttccatgaca aatataacaa ttgatgagat aataacaaag
    10741 tgaaatttga aatcaaaaag gagagcagat atccatgaag ggaatcattc ttgctggggg
    10801 cagcggaaca cgtctgtacc ctttaacaaa ggcaacatcg aaacaattga tgccgattta
    10861 tgacaaacca atgatttatt atccaatgtc tactctgatg ttggcgggaa tcaaagaaat
    10921 tctgattatt tcaacgccac aagatacacc acgtttcaag gaattatttg gaaacggaaa
    10981 tgatttagga ctgcagatcg aatatgcagt acaagaaagt ccagatggat tggcacaagc
    11041 gtttatcatt ggagaagaat ttattggaga tgatagtgtc tgcttggtat taggggacaa
    11101 tatttactat ggtggcggtc tgtctaaaat gttgcaacga gcagcttcaa aagatacagg
    11161 cgcgacagta tttggctatc atgtcaatga tccagaacgc tttggtgtag tagaatttga
    11221 tgagaatatg caagctttgt cgattgaaga aaagcctgct cagccaaaat caaattacgc
    11281 tgtaactggc ctttattttt atgacaatga ggtaatttct atcgctaaag gtatcaaacc
    11341 ttcagaacgt ggagaactag agattacgga tgtcaataaa gcatatttgg ataaaggaaa
    11401 actatccgta gaacttatgg gacgcggatt tgcttggctt gatacaggaa cacatgaatc
    11461 attgcttgaa gcatctacct ttatcgaaac aattgaaaaa cgtcagaact taaaagtggc
    11521 ttgtttggaa gaaattgctt accgtatggg ctacatcgat aaggaacaat tgctggccct
    11581 tgcacagcca ttgaaaaaaa atcaatacgg tcaatattta ttgaatttgg ccgctgaata
    11641 aggagtcctt acagatgaaa gtaacagata caaaattaac agatgtcaaa atcgtagaaa
    11701 tggacgtctt tggcgatcat cgcggtttct tcacagaaag ttattcaaaa gcaaaatttg
    11761 cagaacatgg attgaaatat gactttgttc aagacaacca ttcactatca gctgaagctg
    11821 gtgtccttcg ggggcttcat ttccaaaaag gagaagctgc acagacaaaa ttgattcggg
    11881 tagtcactgg agcagtttta gatgtagtag tggatatccg caaaggtagc ccgacttatg
    11941 gtcaatggga cggatttatc ttatctgaac ataatcatcg acagttgctt gtacctaaag
    12001 gatatgccca tggattcgtt actttgacac caaatgtgaa cttcctttat aagtgtgata
    12061 attactataa tgcagaagca gatgggggca tcgcatttga tgatccagat ttagcgattg
    12121 attggccaat tgatccatca agagcgatta tgtcggaaaa agatcaccgt catccgacat
    12181 taaaagaatt tgaagctgaa aatccatttg tttacggaga aatctaagaa ttaaggagaa
    12241 attttcttat gaaaaacatc attgttaccg gaggtgccgg attcatcggc tctaatttcg
    12301 ttcattacgt ggtaaacaat catccagaag tgcatgtcac tgttttggat aaactgactt
    12361 atgcaggaaa caaagagaac ttggctggac ttcctagcga tcgcgtagaa ttggtcgtag
    12421 gagatgttgc agatgcagaa ttagtggacc gtctagtaaa agaagcggac gcagttgttc
    12481 attacgcggc agaatcccat aacgataatt cattaaaaga tcctttccca tttgtacaaa
    12541 cgaatatcat tggtacgtat acacttttag aagcttgccg caagtatgat gtacgctatc
    12601 atcacgtatc tactgacgag gtttacggcg atcttccatt acgtgaagac ctgccaggac
    12661 atggggaagg cgaaggagaa aaattcactg cggaaacacc atataatcct tcaagtccat
    12721 attcttctac aaaagctggt tcagatctgt tagtaaaagc atgggtgcgt tcttttggct
    12781 tacgtgcaac catttcaaac tgttcaaaca actatggtcc ttatcagcat attgaaaaat
    12841 ttattcctcg ccaagtaacg aatatcttaa gcggtattcg tccaaaatta tatggcgaag
    12901 gaaagaatgt tcgtgactgg atccatacga atgatcattc ttcagcagtg tggctgattt
    12961 tgacgaaagg cagaattggc gaaacctatc tgatcggtgc agacggtgaa gaagataata
    13021 agaccgtgat ggaattgatc ttagaaatga tgggtcaacc aaaagatgcg tacgaccatg
    13081 taaacgaccg tgcaggacac gacttgcgtt atgcaatcga ttcgacaaaa ttacgggaag
    13141 agttaggatg gaagcctgag tttacgaact tccgtgatgg gttggctgac acgatcaaat
    13201 ggtacgaaga acatgaagac tggtggaaaa aagaaaaaga ggccgttgaa gcagcatatg
    13261 ccaaaaatgg acaataatac agagaaaatg tcactgtatt aatggaaaaa cacatgtgag
    13321 gcaggcgctc gcctgtctct tttttaaaat aaaaatcaag gagcggataa tcatatgttt
    13381 ttattaactg gaggaaatgg gcaactaggt acagaattgc gccatttatt agatgaaaaa
    13441 aatctgcaat atgtttcaac agatgcaaaa gacttggata tcacagatgc cgagaaaaca
    13501 atggcttaca tcacagaatt aaaacctgat gttattttcc attgtgcagc ttacacagca
    13561 gtagataaag cagaagaaga agcaaaagaa ttagatgaga agatcaatgt tgatggtaca
    13621 agaaatgtag cacttgcagc aaaagctgtg aatgctcaat tcgtctatat cagtacagac
    13681 tatgttttcg atggaaaaaa caaaaatgta gaatacgaag tagacgacca aacgaatcca
    13741 ctaaatgaat atggtcgtac gaaattatta ggcgaaaaag cagtagaaga gattttagaa
    13801 gactattata tcatacgaac ttcttgggtt ttcgggatct atggacacaa tttcgtcttc
    13861 acgatgcaac gcctagcgga aacccgcgac aaactgactg tagtaaatga ccagtttggg
    13921 cgaccaactt ggacacgtac tttggcagaa tttatggtat atgtcatcga agaaaaagca
    13981 ccatttggta tttatcattt gtcgaacgaa aatagctgtt cttggtatga attcgcaaaa
    14041 gaaatattga aagatacaga tgtagaagtt gcacctgtta cgtcagaaga atttccgcaa
    14101 aaagcaacac gtccgcagta ttcagtcatg agcctgaaaa agacagaaga gttaggattt
    14161 gtcataccaa cttggcaaga agcacttgcg caaatgctgg aagctgccaa aaaaatgaat
    14221 aagtaatttc cttttatcaa atagaatttg gagcatgata tggaaaaaca aacaagaagt
    14281 gcacgacgaa aagaaaagcc aaaagcgaac caggcagatt cgaatttggt gcttgacctg
    14341 tttctaaaac tccgctatct tattggcgtt ttagtcatcg tggtcatcgt tacttttaat
    14401 ttaaacggaa gttcacttgg tgtttgggat aaatatgtgt cgcaaagaga cgatgggaaa
    14461 aaaactgatg tgatttttgg acagaatcgg gaggtgcggt cagacgaatg gctggtgcag
    14521 acaccttttt acctctcaca agctgaagag gactttccgt tggtgaacga ggattattca
    14581 ttaaacggac aaaacatgat cattgcgtat aattctcctg caaaggatat tacggttatc
    14641 ggaaaaccat tcaactgggg attctttttc ctcggaagag atcgaggact ttccttttat
    14701 tgggcgatga aattagtcgt catggtcttg cttggattcg aagtactgat gatattaacg
    14761 aagagaaaaa aagctctatc gctaatcggt gcatttgcgc tgacattttc tccagcagtc
    14821 cagtggtggt tcatgcaaca tgtgggcgat ttgattttct ttactttggg actgatgact
    14881 gctttttatc attatttcta caatcatgat aaaaaatggc tacgaacgtt gatgatgctt
    14941 ttggtggtta ttttcggtat tgggtttatt ttagtgattt acccagccca tcaagtgatg
    15001 ctggcgtact tgctgatttt ttattttgtt ggcctgctta tttactatag caagaaagtt
    15061 tcatgggatc tgttggatgt agtgttgatt ggaggggcag ttttatttat tggcggagtc
    15121 atggttcatt tctggctaac gtcaaaagac gcattgttag cttctttgaa taccttatat
    15181 ccaggaaaac gggtaagtac tggaggaaag tggacgattg gcgaattttt ttctttcctt
    15241 acaaattgga aaattccttt tgaagatatc aactattcga ataactctga agtggccctg
    15301 ttctatcact tttttccaac tgtattccta gcaagtccat ttgtgttatt tggcaaaaaa
    15361 gatagcgaac aaaagttact gggacgtatt ttgatgattt tttgtctgtt ttctatcttt
    15421 tggattaccg ttgggttgcc aaaatggcta gcagaaatca ctctgctgtc ttatgttcct
    15481 ccattaagag ccatccttac ttttagtttt gcggcttgtt tattatcgat ctggtttatc
    15541 gcttatattt ggagagaaga tgttattcct atttggtata aattcatttt gttggcagta
    15601 aatgcatggt cttatttcta tgcgattacg cattcgaaaa tagataatta tttttcggat
    15661 tttgaaacca tcgcagttgc tgtgttggga acagtgattt tagcatttgc attatttggc
    15721 tggagacgct tgttttacct cgcttttgcc gccttagtta tcgtatctgg ctttttcgtc
    15781 aatccagtcg tagaaggtac aggagcgatt tttgaaaaaa cactggcaaa agaaatccaa
    15841 aaaatcgatc aacaagatcc tggtaaagtt tggttgtctg aagatgaatt atataacttc
    15901 accccaacat tgggagtgaa atccttcaat acagtacgct tttatccaga tatagctgca
    15961 tggaaggaaa tcgatccgaa agaagaatac gagaagtatt ataatcggta tgctcataca
    16021 agagctttta tcgctaagga agatacaaag ttcattttag atcgtccaga tatgttttcg
    16081 gtcacgttga attttgaaga cgcagaaaaa ttagggatcg actatgtgat cagtaaacga
    16141 ccattaactg attacaacca attatatgaa gcgcagttca cgcagctata tggaccggat
    16201 aaagacggtt atatgatttt taaagtagaa tatccggatt atccagatgt accacaaaca
    16261 caaactcagg aaaatcagct agtcgtacag taataggcta gctgagaata atcctgaaat
    16321 attagtagaa aggagagtgg agataattca agcaatcgta caaaaatttg tcaacaaaat
    16381 gagtgtcggt ttcttgcttt tgttgctgac gctagtcttt tttgtttgga cagcagggtt
    16441 aggttttcca gcaactttgt ttatatataa caatagttca ttacttatcg cttttagtgt
    16501 ggctattttg ctcattttaa atgtttataa agcaaaaggc gtcgattttc tttatctagg
    16561 tgttgcgctt gcccttttca ttttcttttc ttactttcgg gaattaagaa atgagtcttt
    16621 atttggagat ttactgtttc ctttgattat tttattttgt ttagtaatta aatggatcga
    16681 gtggacaaaa tgggatcgcg gtatttatct gattgtcttt acggtcttcc tcggaatcac
    16741 gttatatcgt gtatttacag agatccaagt accagaagga caaagcatct gggctccagg
    16801 caattcgctt tctgatattt ggatcaatac gaatacaatc ggaagttctt tgatgactct
    16861 tgctttattg atcagtggat ttgccagttc ttttgaacga tggtatgtac gaatcatcgg
    16921 tgcagtagca gttgttgcag cttttctcgg gatttgggtc tgccaatcta gaggtgcatt
    16981 agtcgcgttg attgtttttg cgatattgga tcttttacct aaatcgttga tgcgtgtcat
    17041 tcgtgcgcca tttattgctt acacggttac gattctttta gctttgccga tttcttatct
    17101 ggcggctgtc tctgaaaaag tcaatttgtt taccggtcga gaagatattt ggcataaatt
    17161 ctatcagacg ctaggagaaa aatctgaaca agttttacta ggaatgaaaa catttatctt
    17221 ccaacgagga aaccaatttt taggaaacca caatagttat aattctattt tgaatatcta
    17281 tggattgata ggatttggga ttgctacttt gctgcttatc ctttttattg gtcgcttaac
    17341 gttgaaagca gatttatcca acgggcagat gacttttatc tgggcctttt ttgcggtgat
    17401 gatgcagtca tttatggagg atacattgac ttcataccca tgggttccga ttatttatat
    17461 cctgttggct atggccgttc atcgatatga tgaaccagaa gttgcggagg aattctatgt
    17521 tatggaagag gcatatgacg atatgactta tgatgataca tcttatgggg aacaaccaga
    17581 tagcgctcat tcttccagga tccaacgcta tcattgatag gcacaaaata
//
LOCUS       DACEQH010000055        17013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.056, whole genome shotgun sequence.
ACCESSION   DACEQH010000055 DACEQH010000000
VERSION     DACEQH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.056"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(269..802)
                     /locus_tag="IW833_002061"
     CDS             complement(269..802)
                     /locus_tag="IW833_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ1308161.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1666..2418)
                     /gene="xth"
                     /locus_tag="IW833_002062"
     CDS             complement(1666..2418)
                     /gene="xth"
                     /locus_tag="IW833_002062"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ1308162.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            2531..3451
                     /locus_tag="IW833_002063"
     CDS             2531..3451
                     /locus_tag="IW833_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1308163.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3524..4459
                     /gene="murB"
                     /locus_tag="IW833_002064"
     CDS             3524..4459
                     /gene="murB"
                     /locus_tag="IW833_002064"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ1308164.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            4757..6235
                     /locus_tag="IW833_002065"
     CDS             4757..6235
                     /locus_tag="IW833_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1308165.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            6512..8572
                     /locus_tag="IW833_002066"
     CDS             6512..8572
                     /locus_tag="IW833_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308166.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            8655..9224
                     /locus_tag="IW833_002067"
     CDS             8655..9224
                     /locus_tag="IW833_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1308167.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(9399..10205)
                     /locus_tag="IW833_002068"
     CDS             complement(9399..10205)
                     /locus_tag="IW833_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308168.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            10343..11857
                     /locus_tag="IW833_002069"
     CDS             10343..11857
                     /locus_tag="IW833_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ1308169.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(12260..13243)
                     /locus_tag="IW833_002070"
     CDS             complement(12260..13243)
                     /locus_tag="IW833_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ1308170.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(13248..13895)
                     /locus_tag="IW833_002071"
     CDS             complement(13248..13895)
                     /locus_tag="IW833_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1308171.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(13980..14612)
                     /locus_tag="IW833_002072"
     CDS             complement(13980..14612)
                     /locus_tag="IW833_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1308172.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(14628..15179)
                     /gene="coaC"
                     /locus_tag="IW833_002073"
     CDS             complement(14628..15179)
                     /gene="coaC"
                     /locus_tag="IW833_002073"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ1308173.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTVIEQVMLTLNERNA"
     gene            complement(15176..15913)
                     /locus_tag="IW833_002074"
     CDS             complement(15176..15913)
                     /locus_tag="IW833_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ1308174.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(15910..16899)
                     /locus_tag="IW833_002075"
     CDS             complement(15910..16899)
                     /locus_tag="IW833_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ1308175.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
BASE COUNT         5623 a         3064 c         3322 g         5004 t
ORIGIN      
        1 ggtaaacggt gtccaaaagc ttgcatctgc ggtaataagt ccaaccaaat gaaaaatatg
       61 agaagctatt tttgtttggc tgttcttatt acttgatgca gaacagcttt ttcacaacct
      121 ctccaataaa cggtgttctc ctgccagctc ctcggtcacc agtcacaata tttaataaac
      181 atgagaagct attttttgaa attgctcctt gtgactcgga gtgaaacggc aggctcacga
      241 cctctttgtg tgtttcataa gtattctttt actttatagt cacaagtttt ttcaatggat
      301 ctgtcccgaa ttttttttct tgataaagca ggatttccgc acatgcacga ctatcttcca
      361 atgcatcatg gtgatgatct aattggatgt tcaaattttc gcacacagtg ttcaatcgat
      421 gatttggaaa ttcaggaaac aggcgacggc tacttttcac tgtacataaa gaaagataat
      481 taggctgttc aagatgataa taatctaagc atcccgctaa gacctttgta tcaaaagcag
      541 cattatgtgc aacgactaaa cgattttttt gaaaatatgg ttggatagtt tgccagactt
      601 ctgggaattt cggcgcattg cgtacgtctt cttgatggat tccgtgaatt tggacatttc
      661 gccagaaaaa aggagtctca ggctgtatca atgtatagaa ttcatcaacg atctggctat
      721 tttttaccat gactagcgca agagaacaag cactataagg ctgatgattg gcagtttcaa
      781 aatccatggc aacaaaattc atttctgtaa aacctccttg ggaaattact gtaacatact
      841 tttgaaaaaa gcaaataaat ttaacaaaaa acatagaatt ttttatgtaa tatttgttga
      901 gaaaagctag tttttgaact cattccttgt gatttggagt tagatgggga tcatgacttc
      961 tcggtattaa gtcaaattta tgcaaagttg tgaggaacca ttttcaaaaa aatttcttac
     1021 gactcgaaag taggcgactt tttcacaact tctccactaa acggtgttca atcagctgac
     1081 cctcggtatt agctcaaaaa atagcaaaat atgaggaact attttgccat ttgtttctct
     1141 aatactcagg tcaggacgct gatttcacaa cttctcaata aacggtgttc aaaagcttgc
     1201 atctgcggta ataagtccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
     1261 cttattactt gatgcagtgc agctttttca caacctctta ataaacggtg ttcaaaagct
     1321 tgcatctgcg gtaataagcc caaccaaacg aaaaatatga ggaactattt ttgtttggct
     1381 gttcttatta cttgatgcag tgcagctttt tcacaacctc ttaataaacg gtgttcaaaa
     1441 gcttgtatct gcggtaataa gcccaaccaa acgaaaaata tgaggaacta tttttgtttg
     1501 gctgttctta ttacttgatg caggacagct ttttcacaat ctctcgataa acagtgttca
     1561 aaagccagtt tctcggtatt atgccaaatt tatgcaaaat gtggaaagcc attttcacaa
     1621 aatttttctt aatactcgaa actaaacgac tttttcacaa cctctttatt tcaaatctaa
     1681 ttctactgga caatgatcac tgcccataat gtccgtatgg atctttgcat cagctagtcc
     1741 tggctctaaa tctttgctcg tcaaaaagta atcgatccgc caaccagcat tgtttttcct
     1801 tgaattgaat cggtagttcc accaagagta gacaccttcc tgggttggat aaaaataacg
     1861 gaatgtatcg ataaacccgt tatccaacag tcgagaaaga gccgctcttt cttctggagt
     1921 gaatccagca tttttttgat tcgttttcca gtttttcaaa tcaatctttt gatgagcgac
     1981 atttagatcc ccacacacga tgactggttt ttgttttttt agattttcca aataattata
     2041 aaaggcttct tcccattcta atcggtaatc taaacgtttt aattcgcttt gagaatttgg
     2101 cgtgtaacag gttacaagaa agaaatcgga atattccaaa gtaatcagac ggccttctgt
     2161 atcatggata tcgataccca ttccgtaagt ggcattcaat gctggttttt tagcaaaaat
     2221 cgctgtgccg gaatagcctt ttcgttccgc gtaattccaa tattgataat aacctggaag
     2281 atctaaatca atttgtcctg cttggagttt cgtttcctgc agacagaaaa aatcggcgtc
     2341 taactcatga aaaacttcta aaaaattttt atttacgatg gcacgaagcc cgtttacgtt
     2401 ccaagagata aatttcaata aaagcacact ccttcgaatt aatttcctta cctttgcgat
     2461 actattgtac ctcattttag taaaaaatgg tgtaagatga aagtaactaa atgatccagg
     2521 aggaaagtag atgaagattg gtgtaatcgg tgtagggaat atcgcagaaa aagcatattt
     2581 accaacttat gcgaaaaaac aaggaacact tgatttttat tttgcaacaa gaaataaaga
     2641 aacaaaagaa agaattcaaa aagagtacgg gttccaacat ctatatgaaa cgatcgatga
     2701 gcttttagaa gaaaaaatcg aagcatgtat gatccacgca gcaacaaaag tacattatga
     2761 attagccaaa aaatgtctag aaaatggtat ccatgtttat attgacaaac cactgagtgt
     2821 ccatctaaat gaagttcgtg aattgcaaga attagctgat aagaaccaaa taattttgat
     2881 gatcggtttc aaccggcgct ttgctcctat ggtagaagaa ttgaaacgta tcccagataa
     2941 gcggttgatc cacctgcaaa aaaatcgaat agcggcaaaa gaaacgacag agttcgtcct
     3001 ttatgatttg tttttacatc ttattgatac agctgtgtat ttattagatg aacccatttt
     3061 gagaacaaaa taccagataa gagaaacaaa ggaatttttg gaatatgcca ttttacaatt
     3121 agaaacacaa aatcaaacgg ctattttaac gatggattta gcaagtggcg ctaatataga
     3181 aaaatatcaa gtaacaagta aaaatggtac ttatgaagta aacgatctga cccagcttgt
     3241 cattcaagat gctgatggaa aaaaagtgaa agaatttggc gactggacaa acacgctagt
     3301 caaacgcggg tttgagccaa tggtagaggc cttcttttct cagatccgtt taggtaaatc
     3361 ggaaacagaa ttgcttaaac aaaaagaagt tgtaagaagt catgaattat gtgaggaagt
     3421 gcttaaagat cactaccgtc atcaactttg aacaagactg gcaagattgt attaagcgtg
     3481 atagaataat gaggacgttt gagaaaagaa aggaaaatcc tcagtgaata aagaagaaat
     3541 agttgaaagc ttagccacga ttactatatt gtttgatgaa ccattgatga actatacgtt
     3601 tacgaaaaca ggtggaccag ctgatgtcct tgcattcccg aaaaaacaag aagaagtaaa
     3661 gcaaatcatc gattattgcc gaatccatga tataccttgg atggtattag gaaatgcaag
     3721 caatctgatc gttcaagatg gcgggattcg cggtgtagtc atcatgctga ctgaaatgaa
     3781 acagattcat gtcaaaggaa cgatggtcat tgcggaagca ggtgcctcac tgattgatac
     3841 gacttatgca gctttggcag aatcattgac tggatttgaa tttgcttgcg gcatcccggg
     3901 aagtgtcggt ggagcggttt atatgaatgc aggtgcgtat ggcggagaga tcaaagatgt
     3961 ttttgctgaa gtggatcttt tgcttgaaga tggaacattg aaaactttga caaaagacga
     4021 aatggtattt tcttatcgtc acagtaaagt ccaagaatta cgtgctatcg tattggaagc
     4081 tcgattcagt ttgcaaacag gggattatga agcaatcaaa gcaagaatgg atgagttgac
     4141 cgaattgcgt caatcaaaac agccattaga atacccttca tgcggaagtg tctttaagcg
     4201 cccagtagga cactatacag gtcagctgat ccaacaagca ggattgcaag gcctgaaatg
     4261 gggcggtgca caagtctctg aaaaacatgc tggattcatc gtcaatatcg atcatgcgac
     4321 agcgacagat tatattgaat tgattgcgca catccaagaa gtaatcaaag aaaaatttga
     4381 tgtgtcttta gaaacagaag tgcggatcat tggagaaaaa caagcaatat cagaaacaag
     4441 cagaagttta gccaattaat aagtaatgga acttaaaata agtaaaagag caggacggtg
     4501 gccgtcctgc tcttttttgt ggttgtttac gcaaatggcg caaatcttgt acattttaac
     4561 acaataaaac aaaaaatata gaatatttcc tcataaattt ttccattttt tgcaatattc
     4621 tcaaaaaaaa gataaaaaat gagaataact gatgaaaaaa caagcataat ccatcataaa
     4681 atcagtatag gtgaaaagat gaactttcat ctgcatatca agtaattggt atgaggcatt
     4741 ttttgaggtg taggatatga agttggatta ccttatagaa gaggaagata gagaaaaact
     4801 gctgctagct agatatttag aatttgagaa aaagaattat gtagtgctgc ctaagttata
     4861 tgaaacatta ggtttgtcta aatttaaaac aagaaattat atcatagaat taaataaaga
     4921 atttactgaa ttcaaaacaa agccagagat ccaaatttca gaagaagcag agatccatgt
     4981 cgaacgtctg aatttgacgg atatcaaaaa tttaagaaga atctactttc aacgatcaaa
     5041 aattgctcga ctgttaacgg agatcgtgga ttcaaaagta acgatcgaaa aatttgcaga
     5101 gaatcatttt ttaagcatat caagagccta tgtaaaaaga agagaattag tctcttttct
     5161 tgaacagcat caaataaagt taaagaaaaa catattagtt ggggaagaaa taaacatacg
     5221 gaacgtatta tttggggtat atttcggggt ctttaatggt ttttctatgc catttagaga
     5281 aagcgtcatg aatgaagcaa agagtatcgt gcaatatttt gaatatctgt tcaatttaaa
     5341 actgtctgca acaaaacgaa tcaaattcaa tttgctaacc actattagtt tgttgagggc
     5401 ctatgggaat caaccaatcg gaaaacgttt cttttcagat gaatacttta agagctcaga
     5461 aggaatgaag atcatcaagg atttatcaca tatttctttt cttcatatcc ctaaagagca
     5521 gatcattgat gagatttctt atatcatgtt gtttttgatc acggaagaag aatttgaatt
     5581 agatggcgca tttggacttg cggaagctca ttttcatgat atccacatga ttagtcaaaa
     5641 aatgtcagtg gaattgattg atcaattacc attttcgatt gaaatctctg aagaagaaag
     5701 actatatata cgaaaagaac ttcgtatggg tatgatgcat gtcaatcgaa aaaattggat
     5761 attcgacttt gagatgacta gctttacgtc taaaaagcag ctacagtttt tcttcgagac
     5821 gtatccaata ttcagtacag caatccaaaa ggtcatacaa gagaataaac gctatttctt
     5881 gggaaaagat gaacgtttca tgattcgctt attttatgat tatctctttt tattggtcgg
     5941 tatttgtccg gtatcatatt ttgaagcacc gattcatgtc tgtattgatt tttcgcaagg
     6001 atctgcttat accgattaca ttatttctca agtgaaagga tttaaaaatt acaatattgt
     6061 gattgagagt cgaatcactt ctcgtacgaa tatttattta tctgattgtt tacttgaaaa
     6121 atttaataaa gaacagataa tttggaaaaa tccaccgact ccagatgact gggaatattt
     6181 tggaaatacc atcatcagag aaaaaaatag acatttaact gaaaaaatca gctagtattt
     6241 atggatggca gctttgtctc acatatttat tagctgattt atgttttttt caaagaaaag
     6301 gtaactagat ataactagat ataactagat atattactta acaaaattaa aaagtacata
     6361 agttattatt ccgattttct tgaaacaata aaaaatcaaa aaaaatactc tcctaatagt
     6421 ccatcttctt ttgattttat ttatataatg aaatcatctt atgaaaatta ctacttcatg
     6481 tttagacaag agtagggagg actaagaata aatgtgttca aaaagacgtt caaaacaact
     6541 aaataaacac gcttgtcgtt tagataagga acggtcgtgt tctgaaaaac actacgttaa
     6601 gtttatgatg cgcacgatgc tgatcagttt atctgtaggt gtctatatgc atgtacccgt
     6661 tcaggcaacg acacaaacca tcaaggtcgt acgtacctct gtttctgaga ccactgattc
     6721 ccctatcact attactcata ctttcactga tgcaaatgga aaacagctac ttcctgcatt
     6781 gacttcctat aataaagtca gttatcctcc aacgataaac cactactggt taattcctaa
     6841 caaaacatta tggacatcgg aaaccggaag cagtgtgccg ctttctatct tttccgatat
     6901 gactgatcct acaaaaggtg atcagttaaa acagatcact gatattttga atcatgcttt
     6961 gacagatata aaggacggtg gaagcgcaca agtttcttat gtatatgaaa aagattattc
     7021 acagtttgaa agaaagaaaa tcgatgttct ggcagggaca aaagtttcga tatctgatct
     7081 ggtagaaagg ctgaagaacg cagatggaac acaaggagat atgtccaaag taatttcaaa
     7141 caatgggaat atcgtggata cgactaatac aggaacatct actatttcct tgtcctattt
     7201 tgatccaatt gctttaaaaa caatgactgc atctgctgtg atccatgttc gtgctgatca
     7261 aacagctatc aaaggaaaag atcttcactt aagaacagga gagagttacg cccctaaaga
     7321 tcttgttgaa tatgtaacaa aaagcgatgg aacttcagga aataaagaag aaatcaaaat
     7381 gaacgggaca gtagatacta caactccggg agaatatccc gttgtattaa cttatataga
     7441 ctcgaaaaca tcaaatgtgg cagaaacaac agctgtagtt tatgtagaag catctgctgc
     7501 ttcatcgact gtcatcactc attttataga tagtcaaaca aataaagaac ttcaagcaga
     7561 aacacatatg agtagtgtaa atcgtattcc tgatttgcgc ctacctgctg aattttctga
     7621 tcgagtgcca aatatagatt tatcgactta tgaacaaaat gggaaagtca cttctatttc
     7681 tgattggatg caagcaaacg gattagacag aaaaagtcac tggtcggaga tccttgaagt
     7741 acttcactca gaactgatga ataataggaa gcaatcagga ggggaaatta cctttactta
     7801 tgtttatata ccagacaatc gttcattaga aggtcatgat cttattgttc atccaaacga
     7861 aaaaataaac aaagaagagt tgatccaatc agcaacaaat tccttaggta aacccgtacc
     7921 gttaaaggat gtatcggtta caatcaatga agtaccattg gaggatggtt atttgaccaa
     7981 acaaacaggt gattttacga ttatgtatag ttatcatgac ccgtattcac tccaagaact
     8041 ccatgctcag tcactattac atgttttacc attaaacaaa cagatagatc aaaaagaaca
     8101 aaaaagacaa gaagaaagct tgtcagaaaa aaaacaacaa atggctcatg cagtttctcc
     8161 aacagggaaa gtaaaacaaa caagaagcct agagttagca agaatatcta gaaaaatctc
     8221 agaatctaat aaaacgataa ggcaagaagc tcatttggcc ccacaagttt ctggattgga
     8281 aaatgaaatt ttagagccta aaataaaaca acaatcagaa aaagaatttg ttgctaaaca
     8341 gtcggaagat cagaaggaat cagatgacca gaaggaaacg aagaaaaaac gcttgtcaac
     8401 tattgattcc accgatgagc atctgcagcc gcaagtacca cttcctagtc cgggaacacc
     8461 cgcacctggt ggaggtattc cttcttctgc tttaggagat ttcatgcgag gtatggcagg
     8521 aacatgggta tacgctaatc gaaatgaatt tgacgcagat gactggttct gatttatatc
     8581 tctatctgtt atagtcaaat aagtagctga gtcatatgtt atttggcata atggagacga
     8641 ggagggatag aaaaatgtta gataaaacgt taccttacgc agaaatctgg atgacaagac
     8701 cacttcaaca aaatgttcca atcacgcctt tagcggaagg ttttgaattt cgattttatc
     8761 aaaaagaaga tgaatctgct tgggcggaga ttgagacttc cgttggggaa tttcaaacga
     8821 cagatgaagc acttgagtat tttcaacaaa actttgctcc ttatcctgaa gaacttgaaa
     8881 gaaggatgtt ttttgtagag acaaaaaaag gagaaaaagt agcaacctgt accgcttggc
     8941 gaaaaaaatc aagagagcaa gtgtatccgg tttttcattg gttagcagtg aaaccagatt
     9001 accaaggaaa agggctcgca aaagcattga ctgccagagt cttgcaagaa tttcctaccc
     9061 tccatacgga cggaccaatc tatcttcaca cacagacatg gagccatcag gcgattgctc
     9121 tttataaaaa aatgggtttt acttttattg ctgaaaatct agacggcaca cctaatccag
     9181 attatgaaaa agttatgcag gttttaagta aattagataa ataacgaaat ggtgaaataa
     9241 gtcctcagta ttgggataaa aaacgaaata gtcattcctc atatcttact tttttgagtt
     9301 aatactatcg gataactaat ggcatgccat acattcaaaa aagaaggggc agtgacaaaa
     9361 gtattgtcac tgccccttct cttgtttttt agcatgtttt atcgtttgat ttttttagga
     9421 ataaaattct tgatgaacgt aaatttttgg taatcgtttc gactatagag gtaaccaaat
     9481 acactgaaaa caactgctcc aataaaatta acaaataaat ccttcatagt atccatgatt
     9541 cccacgtcta agtaaccatt ttggatgatt gttttatctg ttttaccatc tgcattttga
     9601 ctttcaatga ttgtccgagt gatatctgga acatgataga cagtgtttcc gtctttactt
     9661 aactccacac tattgatttg ctggatgatc gtatcttttt gcatatcgaa ttgcagtaat
     9721 ttatctgctg aaaattccac aaactcccac ataacaccaa ctgtcatcga aaaacagaag
     9781 gaaacaactg ctagtaataa aggagtcaag ttcatggttt ttgtattttt attgagcaaa
     9841 taaacaaggg aaaagccgac acccccagct aaaaatccgc tgaatgcatg tagaaaagta
     9901 tcccacattg aaaaatagcc ataaaaatca cttaactctc ctaaaactgt tgaactaaaa
     9961 ataaaagata acaaaatgat ttctagcata tttggaatct gtatctttaa tatcttttcc
    10021 agaaaataag ggatttcaaa taaaattaat gtcagtaata aaagcattgt atttccccag
    10081 tgttggttcc agacctgatc cactagcata ataacgacga tgatacgtaa taaaatatag
    10141 attccgtttg tgattctacc tgcagttttt gtactgttcg taaaaaaggt tttcaaatga
    10201 ttcataaaat aactccttca tgtatatcct taattatact aagaagcgtg tgtttaccaa
    10261 agctatatga cttaaagaaa aacagagttg atattgtgaa aaaaatcact taatatagta
    10321 taaggagaaa agggggatac aaatgaaaaa attagttgct ggtttattaa cagccgttgc
    10381 acttcttgct atgagcgcat gcggcgcaga tcagaaaaat acagcgcaga caagttctag
    10441 tacgaaggaa tctgccgaag tatcatcagg cgcttctgaa caagtatata cagatccaaa
    10501 agaaatgaaa gaatcttatg atgtcgtgat tattggagct ggtggtgcag ggatggcagc
    10561 agctatcgaa gcaaaagatg ctgggttgaa tccagtaatt ttggaaaaga tgcctgttgc
    10621 cggaggaaac acatctaaat catcaagcgg tatgaatgct tcagaaacaa aatttcaaaa
    10681 agaagaagga atcaaagact cgaatgatgc cttctatgaa gaaacgctaa aaggtggtgg
    10741 aggaacgaat gataaagaaa tgcttcgata ctttgtcgat cactctgctg atgcggtcga
    10801 ttggctagat caaaacggta tcaaactgga caacttaacg atcactggag ggatgagtga
    10861 aaagaggaca catcgtccgt ctgatggctc ggctattggt ggatacttag tggaggggtt
    10921 attgaataac gtccatgagc gggaaatccc tatttttgtc aacgcagatg tgacagacat
    10981 taagaaaaat ggggaaacgg tcaatgaagt gactgtacat gttcaaggag aagaaccaaa
    11041 agaggtcacc ggaaaagcaa tcgtagttac cacaggcggt tttggtgcca gcaaagaatt
    11101 tattgaaaaa tatcgtcccg atttagctga ttatattacg acaaatcaag aaggatcaac
    11161 tggagatggt atcaccatga ttgaaaaatt aggtggacaa accgttgata tggataaaat
    11221 acaaattcat ccaactgtgc agcaggatga gggggtcttg attggtgagg ttgttcgcgg
    11281 agaaggagca atcttggtcg atcaagaggg gaaaagattt gtcaatgaaa tggatacaag
    11341 ggataaagta tctgcagcaa ttacagcatt gcctgaaaaa tcagcttatt tgatttttga
    11401 ccaaggcgtg cgtgatcgtg ccacagctat tgaattttat gaccaaaaag gatatgtcac
    11461 aactggaaag acaatggaag aattagcaga aaaaatcgat ttaccaaaag aaacactagc
    11521 aaaaacagtt gatggatgga atacggatgt tgcggagaaa aaagatgctc aattcaaccg
    11581 tgccacagcc atggaacatg acttatcgtc tccgaattat tatgcgatca aaatcgcacc
    11641 aggaatccat tacacgatgg gcggtgtgaa aatcaatacg aacactgaag tattagataa
    11701 agaaaaccag ccaattaggg ggttatatgc tgctggtgaa gtcacaggtg gcttacatgg
    11761 aaacaaccgg atcggtggaa attcagttgc ggagatcatt atttttgggc gacaagcggg
    11821 catacaagca gctcgatttg cttcggaaaa tgaatgaaat agaacgtaaa taaaaagagg
    11881 ttgtgaaatc agcgttttga gctaataccg agggtcagct gattgaacac tgtttagtga
    11941 agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaagta aaaacagctt
    12001 cttatgtttt taacttggtt ggacttatta ccgcagatgc aagcttttga acaccgttta
    12061 ttcggaaaaa gagcgtggga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt
    12121 ataaacggcg gaagcagaag aaattccttc ggaaataagc tgaaattcac aaaaatttga
    12181 aaagcaattt tcgtgaattt cttcttattt cttggagtta aacgcttctg tcccagcctc
    12241 ttttttgatc agcatgattt tattcagtta tcttcaaaat gatttttccg atgtttttgt
    12301 ttgcttccat atattcatgc gcttgtttga ctgcgctgaa ttggaatgta cggtcaatga
    12361 ttggccgaat cttgccattt tctagaaaag gcccagcttt ttgcataaaa gtttgtgcga
    12421 gctctgcttt atactgatca cttctaggtg tcaataatgt accaagcagt tggacacgtt
    12481 tcccaatcaa tacaccaagg tctatctcag gcacgatggt cccacctagc ataccaatca
    12541 agacccaacg tccatcaacg gctatagaag ctaaattctt ttcccagtaa gaagcgccga
    12601 tgaaatccaa aataacatcg actccccgac cttcagtgat ttttcgtacc tcttcttgaa
    12661 agtcttgttt tttatagttg atcaattcat ctgcaccaag ttctttacaa aaggcgagtt
    12721 tttcttctga tcctgcagta acgatgattt tcgcgtgaga aagctgtcta gctaattgga
    12781 tcgctgctgt tcccacacca cttgctcctg catggatcaa gacagtttcg ccttcttgta
    12841 attttccaag ccagtaaagt gtttgataag cagtcaaaaa aacttctgag attccagcag
    12901 cagaaacata gtctagcgta tcagggagaa ggattgcacg atcggcaggc atcacagcat
    12961 attcggcata accacctaga ttgactaatc cgtatacttt tgtcccagga gtaaaagtgg
    13021 agatatcact tttattctca actacctctc cagctacttc gactcccaaa atagggtagg
    13081 gaggtttcaa agagactgat tctcttttca taatatctgt tcgattgaca gctgctgtat
    13141 ggactttgat cagtaattcg ccttgttttg gtttaggaac aggcacttca atttgttcta
    13201 atacatctac agaaccagga tgcttgattg aaatggcacg catggatcta ttcctccttt
    13261 gcccattctt ggaatgctgt attcaactct ttgctacctt cagaaagtac tagagtcgta
    13321 cgagaaatat ccgtttcttc cactagagcg gcaagtacag cagcaactgt agagcgagtg
    13381 atcgtttttg atgccttctc attaggctta gttaattgaa tggaagtaac ttcttcatcg
    13441 ttagtcaatg tgactggttg aagaatgaca aaatctaagt tagttcgttt cagccattca
    13501 tctgcataat gtttagtaat ataataatct gtcatggact caggccattt tgctggttca
    13561 tctgcataaa cagcactgac catcagatag cgagagacat gcgcattttc agctgcgatc
    13621 actgttttga ttgcgccatc caagtctact tgcagaagat tctttccttg agagccggca
    13681 gcaaaaatca atacatcgat gcctttgaat gcttcggtca ttttttccac ggaccaagtt
    13741 aaatcaaaag agacatacga aacattctct ttttttacta atgtttggga agcaacatct
    13801 ctcacccctg caaaaatttc atgggaactt ggagctaaat cttttatcag gtgtcggcca
    13861 atttgtccgt ttgcacctac aacaaaaatt ttcatttgga tcatcctttc gacgtcaaat
    13921 ctttccattc aatagtaaca ccaaaagaga aaaaaatcag agatcggcta cgatgactat
    13981 cagttagtaa gagaagggtg cttgaagtat cttaacaaga cattgcatac tgctgcagtc
    14041 gcaagacctg cgacgattcc ttcaggtata ccacttgtaa aaataaccgc tagaatagct
    14101 gaataaacag catttaaatt tgttccgatg actttggcat aatcttgctg gaataaaaag
    14161 tagatcatat tcatcactaa aatcgtattg gtcaatgaac cggaaagtcc agctaagaat
    14221 aaagaaacgg aatctgcttt ttgtttcatt agtttgcgca gacctttata aacgaaataa
    14281 ggaaccaccc caatcaagat gcgaggaata aacgcgatca gcaaggcttt ccagcttcca
    14341 tgttctgttc cgatgactgg gataaacgga gaaaagacaa aagacaaagg tgtttggata
    14401 actgtgctac ggatcatgct gatcagtcca aatgtaccgc caagaaaagc acctagtctt
    14461 ggtcctaaaa cgatcgaagc aatgattaca ggaatatgca tggttgttgc attgattgga
    14521 ccgataggga taaaacctaa aggcgtaaca gctaataaaa tcaaaatagc taaaaacatc
    14581 gcagttaatg taaagttttt tgtatttttc ataagtactc cttttttcta agcatttctt
    14641 tcattaagag tcagcatgac ctgttcgatg accgtctgaa tatcagctaa ggcacctttt
    14701 ccaaaatctc cacaagctaa aagagattct cttggttcaa tttcctgata gccgacagat
    14761 ctcaatgttt ccagattacg ctgagtgatc gggttttgat acatattcgt gttcatggca
    14821 ggggcaatca attttggtgt tcccactgga agcgccattg cgactgttga aagaagatcg
    14881 tctgcaatgc cgtttgctag cttgccgata atgtttgctg tagcaggcgc gatgagaaac
    14941 aagtcagctt gttttgctag ttcgatatga ttgattacct gcggatttga ttcttgcatg
    15001 acgtctgtat ggactgcgcg cttcgacaga gattgcaagg ttaatggcgt gataaaatgt
    15061 gtgctgcttt tagacataat cacatctacc tgggcaccta atttgacgaa ttgacttgta
    15121 agatcagcgc ttttgtaagc agaaatactt ccagtaacgc ctagtaagat ctttttcatt
    15181 ttgattcctc cagtctaatt ttctcagtaa tcaatgcagc aatctcagat ttactttgag
    15241 cttcttccac tgttccatct ttagataaga gatagccgtg gtgtgtctcg ccatggattt
    15301 ccgtcaaatc atttgcgaaa ataaaatcag ttttgttatt tatcagacta ttttttgcga
    15361 ctgccagaag agattccttt ggtacatcta ctaatagttt gaaaccaatg atttttgctt
    15421 ttggctgcca ttcacgtaga taagaaatga tttttggtgt tttcttcaaa acgagcacaa
    15481 gatgctcggt gttagaagag atctttgaaa cagtggtatc tgtctgttca ttttccgcaa
    15541 accaattgtc cagttcttct tgcgttgaag aagtgggtag gttttttgcc agctgttctt
    15601 ctgaaaaact aaaagcagga gtaaaatcac tgactgccat actgtggata atcgcatcgt
    15661 agtgttcttt tttcgttagt tcttctaatt gtaaaaataa gtccagtgca gattcgatcg
    15721 tataaatcgt tatattggag tgtcttttcg gtttcagtgc ttgtttcgta gtcacgtaat
    15781 cgattgtggt atccggattg gcagctaaat aatcagctaa tgcttgccct aatcgtcctg
    15841 tcgaatgatt tgtaatagca cgtacttgat cgattttttc agtcgttcct ccagctgtga
    15901 ttagaatctt catttatggt cacctacatt tactagtgtt cttccttggt gtgttccagc
    15961 aagtagttta gatgctgttt caggcaattg atccagagta atctcttgga cgaccagttc
    16021 atctgcaatc tgcaaatccg ccatctgttg ccataaagaa agacgttgat ctattggtac
    16081 attgacagaa tcaattccaa tcaactggat gtttcgcaga ataaaaggca agacagtcgc
    16141 gtttagtttg attcccccag cattgccaca taaagctact gcaccgttat aagagatcaa
    16201 tggaagaaga ctgcttagct gctcgccacc tactgtgtcg atgacatagt cgatttgctg
    16261 ttttcctaaa gcttttgttg tctctggcag aaattcatca ggtcgtacga cttgcaaagc
    16321 acctagactt tttagccatg tgacagactc ttcttttctg gaaaaggcaa tgattgactg
    16381 gaatcctagt ttatgtaaca gggctatcgc tgtactgcct acaccgccag aagcaccagt
    16441 aacgacaact tttttatcgg aggtcatacc ttgacggatt aaagcgttca cacacaacat
    16501 agcagtaaac cctgctgtac caaggatcat cgcttgacgc agcgtcatat tttttggtag
    16561 aggaactaac cagtctcctg gtactttttg atattggcta taaccgcctg gatgggtgac
    16621 gccaagccca aaaccagtag caagtacaag atcttctttt gaaaaacgtg gattgttact
    16681 ttggacgaca gttccggata aatcaatccc tggagtcatc ggatatgttc gtatcacctg
    16741 accagactcg ctcattgcca atgcatcttt ataattgaca tcagaatagg caatttttac
    16801 caagacttca ttttcagcca actcagtaaa tggcagatca ctgattgctg tatggaaaac
    16861 aggttccttt attacctgaa aagatcgaaa agcgttcatt cgtgagacac tccttttaaa
    16921 attttctttt atcataccat aaagcctact cttttattct tgttcttccg taaatttaga
    16981 caaaaattag gtgaaaaata aagaatcaaa caa
//
LOCUS       DACEQH010000056        16887 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.057, whole genome shotgun sequence.
ACCESSION   DACEQH010000056 DACEQH010000000
VERSION     DACEQH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.057"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(39..405)
                     /gene="ssrA"
                     /locus_tag="IW833_002076"
     tmRNA           complement(39..405)
                     /gene="ssrA"
                     /locus_tag="IW833_002076"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(576..1349)
                     /locus_tag="IW833_002077"
     CDS             complement(576..1349)
                     /locus_tag="IW833_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1308176.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1493..1843
                     /locus_tag="IW833_002078"
     CDS             1493..1843
                     /locus_tag="IW833_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1308177.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1824..2540
                     /locus_tag="IW833_002079"
     CDS             1824..2540
                     /locus_tag="IW833_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1308178.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2540..3988
                     /locus_tag="IW833_002080"
     CDS             2540..3988
                     /locus_tag="IW833_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1308179.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4058..4567)
                     /locus_tag="IW833_002081"
     CDS             complement(4058..4567)
                     /locus_tag="IW833_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308180.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4557..5282)
                     /locus_tag="IW833_002082"
     CDS             complement(4557..5282)
                     /locus_tag="IW833_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308181.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5540..6880)
                     /locus_tag="IW833_002083"
     CDS             complement(5540..6880)
                     /locus_tag="IW833_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1308182.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(7047..9167)
                     /locus_tag="IW833_002084"
     CDS             complement(7047..9167)
                     /locus_tag="IW833_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1308183.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            9397..10575
                     /locus_tag="IW833_002085"
     CDS             9397..10575
                     /locus_tag="IW833_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ1308184.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            10591..11349
                     /gene="aroD"
                     /locus_tag="IW833_002086"
     CDS             10591..11349
                     /gene="aroD"
                     /locus_tag="IW833_002086"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ1308185.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            11372..11857
                     /gene="ybaK"
                     /locus_tag="IW833_002087"
     CDS             11372..11857
                     /gene="ybaK"
                     /locus_tag="IW833_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ1308186.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11928..13181)
                     /locus_tag="IW833_002088"
     CDS             complement(11928..13181)
                     /locus_tag="IW833_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1308187.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            13298..14647
                     /gene="gdhA"
                     /locus_tag="IW833_002089"
     CDS             13298..14647
                     /gene="gdhA"
                     /locus_tag="IW833_002089"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ1308188.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            14759..16105
                     /locus_tag="IW833_002090"
     CDS             14759..16105
                     /locus_tag="IW833_002090"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ1308189.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(16413..16799)
                     /locus_tag="IW833_002091"
     CDS             complement(16413..16799)
                     /locus_tag="IW833_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1308190.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5428 a         3047 c         3451 g         4961 t
ORIGIN      
        1 aaggatttaa gcatctagcg aaatctgctg aaatctcaca atggagacgg cgggagtcga
       61 acccgcgtcc aaacacatcg ccacttaaaa atctacgttc atagtcaact catttaagga
      121 ttcactttac agcttgccga gtgacaggca ttctgtatcg ctagtctgat gttctctttt
      181 tcaacttaca gacggaaagt tgaaacgtat cccactaaag ttaggaccct tactcgagca
      241 catgggcgat gccgagagga tctacgctaa ctgtttttag gcagctaaag cgtaagagtt
      301 gttttcgttt ttagcagtta tatttaactg taacgttttt acgtagacgt aacctacgaa
      361 acgcaattca agctcgactg tgcctgtcga atccgtaacg tccccgttag ctaacaaggg
      421 ttacgtgtat ttttcataca aaattagtat agcatatatt gggataaatt caataaaaaa
      481 gcgaatcata tcccattatt tatataataa ctgccccttt cttaagaaaa aataagcgat
      541 tttcttaaga aaagaggtag cttcaaaaaa agatcttaaa tcagatcaaa atacttcaat
      601 gcgtggacaa ttccgccttc tgtatttttc tttgtgataa aggtagaaag ttcttttaat
      661 ccgtcatagg cgttgcccat agcgattttg ttgtcgcaag cttccaacaa ggcaaaatca
      721 ttcggaccat cgccaaatcc aaaagtagga actttttcca gctgaagttc tttttgcaga
      781 cgtttgacac ctgccccttt tgagattcct ttcttcactg tatcgatcga gtaaggacca
      841 ttccgataaa aagttagctc aggaaaatgt tcgtgatagt attcgtcacc tgattgagat
      901 aagatcagca acatatttac tgtgttttct ttaaagccta aaggatcaat ttccggaaca
      961 tctgaatgaa tgaagtcgta agcattgatc attgtttggc tatgaccggt acaccagatt
     1021 ttctgatcgt tatagtaaga aagttcatga ccgttcaact gtacatgatc atacattttt
     1081 tgacattctt ctgaagaaaa tgtttgagaa tagatttctt cgccgtctac tcgaatgaaa
     1141 gaaccattca tggtgaccgc agaagtaatg cccgcatctt ccatgataga gtggatttct
     1201 gcttctgtac gtcctgtagc gatgacgggg agaacattgt tttcttttaa tgcgttcata
     1261 gcttttgcca cttcgggtgt gatttttgat ttcgcatcta gtaatgttcc atcaagatca
     1321 aagaaagtga ttgcacgata atttgacaca aataaaactc cttttttatt ggaataagtt
     1381 tatcttaaca aagaatgaac aaaagacaaa cctttcgtga agaattcctg aacatattgg
     1441 ttcagtcaac aatttagagt atactataag aagagaattt aggagggaaa gaatgatttt
     1501 tttcagtatt ggtatcttat tcttaataat tggcagtctt tttcggattc tgccctcaaa
     1561 aggaaatctg cctttttatg ggtatcattc gcctcttgcc gcaaaaacag acgctcattg
     1621 gcggctggct caaaagacaa gcggaaactg gttttttctt atgggcttac tgatggcatt
     1681 gatcggctat tatttaaaga catctgggca tacgaattat tttctgattg aaatgcttct
     1741 gctagtattt ccaattatgc ctatttttat tatgacagaa aagaaattac agaaatatga
     1801 cctggaaact ggagggaacg ataatgaata ttttaatgat tgaagataat caatctgttt
     1861 ctgaaatgat gcagatgttt ttcttaaatg aaggttggga agcaactttc aagtatgatg
     1921 gaaaagaagg gctcgatgcc tttttagaaa acccacaaca ttgggatatg attacactag
     1981 acctgaattt gcccacactg gacggtatgc tagtaggaag agagatccgc aaagtttcaa
     2041 gtactgtgcc gattatcatg ctgacagcta gagattctga aagcgatcag gtgatcggct
     2101 tagagatggg ggcagatgat tatgtcacaa aacctttcag tcctctgacg cttattgctc
     2161 gaatgaaagc tttgcatcgc cgtagtgagt tggcagaagc aagagaagaa gcagtagttt
     2221 cagatgatca ttttgatatc gagactagtc atttcaagat gaatacgaaa acacgagaag
     2281 cgtatctgga caaccggcca attgaaggac tgacgcctaa agagttcgat ctattgttta
     2341 ctttagcaaa aaaaccacgt caagtattct ctcgggagca gttgcttgaa ttagtctggg
     2401 attatcagta ctttggtgat gaacggacag tggacgccca tatcaagaaa ttacgacaaa
     2461 aaatcgaaaa ggtcggacca caagtcatcc aaactgtttg gggagtcggc tacaaattcg
     2521 atgattctgg tgttgcctaa tgcgttattt gtatcagcaa ctcttagctt tctgcggaat
     2581 ggtcgcgttg atcttattga tcgttggagt ttcttttaca caactgacaa aacaaacgat
     2641 tgaagaaaac aattaccagc aactgtttgg atatgctgaa tcagttgaaa agacaacgca
     2701 aacctatgcc gatacgctcc cgcagtttag tcaagatcag gcgtttcaaa atgcattgct
     2761 gctgacagag caagtactga cagaacaaaa tgtgaacttt atatttatcg ataaaaacga
     2821 acgtgtcatt tatccgacta atacagcttt actgaatttt accttaacag ctgatcaatg
     2881 gaaaaatctg aggaatggac gtcaagaaaa attcacatcg aataaaaata ttttaggaca
     2941 agcacaggcg acctcttacg cacttgtgcc atttaatttg aatcatgaat tctatggagc
     3001 acttgtagtt agtcaacctg cgcgaaatat cgataacagc gttcgtccaa ttacactgaa
     3061 cctgttcaaa ggatttattt tttccagtat cgttgcagtt attgccagct atgcttttgc
     3121 agcattccaa gttaagcgta tcaatcggtt aaggaatgcg acgaaagaag tgacgaatgg
     3181 taattttgat gtacagctcc ccgtacatga caaagatgaa tttgatgagt tagcagatga
     3241 tttcaataaa atgacgaatt cattgaaaga atctcaagca gaaattgaag aacaagaaaa
     3301 tcgtcgaaga caatttatgg cagatgcttc tcacgagatg agaacacctc ttacgacaat
     3361 caacggactt ctggaagggt tagaatacaa tgcaatccct gaaaatcagc gagaaaacgc
     3421 gattaaattg atgaaaaatg aaacagagcg tctgatccgt ttggtgaatg aaaacttgga
     3481 ttatgaaaaa atccgtacga atcaaatcag tatggtcatt aaaaaattca atggaacaga
     3541 aacattgaaa aatattgttg cacagttaga agctaaagca gaagcagcag gagatacatt
     3601 gacattgaaa gccgttgatg atattgatgt ttacgctgat tatgaccgat ttgtccaaat
     3661 catggtcaat attattcaaa acgcgataca gtttactgaa aacggccaaa tcacgattac
     3721 tttagaaaaa ggctatcttg aaacgatcat cacgattgaa gatacgggaa ttggaatgtc
     3781 tgaacaacaa atgaaaagta tctgggatcg ttattataaa gtagatcctt caagaaaaaa
     3841 tacaaaatat ggtgaatcag gattaggttt accaatcgtg cagcaacttg tacgcttgca
     3901 taaaggaaaa ttagaagtag aaagcgaact aggaaaagga acaaaattcg ctgttcgttt
     3961 gcctgacgta gaaatcaaaa aagagtaaaa aacaagcggt ataaaagatc atgaatcgat
     4021 cttctatacc gcttgttttt actataaaat gattgattta gcccgcgggc tctaaacgat
     4081 caatataagg actttgagca atagtagcat catgagtaaa gagtaagata ccttgatttt
     4141 cttcatgagc taaataagaa agcaagtgga gtagctcttg gatctccaat acactaagtt
     4201 catcaaatgt atcttcaata acaatattgt ttcgcttgga aagcaaggca tgaagtaatt
     4261 gcagctttac tttttcaaaa gacgtcaact cagctgccgc acaattaagc aaggaaggag
     4321 agatatcaag tttttcttga taggccaaac gatcttgttt gatttctttt tccggtacat
     4381 ccaacaggac atggtcttta agattcagat aaggaaacat cggccaattt ttttggatga
     4441 tcgcaaaatt atccagtttt gcaacaagaa cctctttttt caagtgctcc aatattttat
     4501 gctgtttttc gagaggacac aaaataacgg tcaagtgttc agcatgaatc aaatattcag
     4561 gtttcattac gtccaggcct cctactttgc aaatgatgga taaaaaaagt gaaagggaat
     4621 agactaagaa tgagacagca tccactgagc ccaataagag tttgggcaaa tcccttaagt
     4681 atatctgtga aaaaaagatc attttggaaa tcgatggtaa aaaaagtctg ttgatccatc
     4741 ggaattgtaa gtccgcttac cgaagtcaag atatcacgga tatctggcag ttcagtagtc
     4801 tttaagacaa gaaaattgat cctttgaaga agcgcttcat aaactgactg gaagcttacg
     4861 atccacaata aaaccaagat acaacaaaga aagagaggaa agaacagctg ccagaaaata
     4921 aaaaggaata atttggtttt agaaagacca gtcaggtgcc aattgatcaa ttcctgcttg
     4981 ttttgcctga ctgaatagta aaagaaaaag ataaaaagga aggcaaatag aaaaaataaa
     5041 ctgaatatgc cttttatata aaatcggcta gctaattcta aagccggaag aagctctgta
     5101 gatgcagaag cgccaataaa tgaaagccga tcttggactt gattggctaa agccgattgc
     5161 aaatggtaaa gagtcaggca tgctatcaat aaaaaagcag aaaagcttga aaataaacaa
     5221 tagagtattg tctgcttttt gtaataaatt aatcgagcag tagcatagcg gattattttc
     5281 atacttttca cctctataaa aagtatagaa aatgaatatg aataaagtat gaaattcctt
     5341 tagtgaaatc acgaattaaa accagaaaat attttgtaaa aagcaagagg ttgtgaagaa
     5401 gctgccctat tctattaccg tagatgcaag cttttgaaca ctgtttattc gaaaagagga
     5461 gcagcgacaa taattttggg ctcttcgtca atgagtgttg gtgaagaaat tcaatagaat
     5521 aatcgagata ctcacacggc tagacagcta tcttgtctag ccgtgttgtt gttctggctt
     5581 tagtcagttg ggtagtgttc ttagttttct tgaaatattt tcctcgatat aaaaagattc
     5641 gttctcggta gttttgaaag tttctaaagc cataggcaat tcgttttagt actttgatat
     5701 ggttgttcaa acattctata gggccgttgg aatagggaac tcgcaacgct cgtttgattt
     5761 ctgttcgata tttcttgaag gtcgtgaaca ctggttgata gtgctctggc aactccttgt
     5821 agtcttcttt taggagcgcg acgaattcag gaacatcttg tcttctgata gcggaaagaa
     5881 aaacctggta gacttcgtag cctcgcttta aggagtcatc ataatataat agccgatcga
     5941 ctatctccgc ttctgtcaga tgatctcgaa atgacggacg ccagtagcga tgctgaccat
     6001 tcaattccca cgcattcttt tgaagtagtc tccagtattt ctttaagtgc ttcgaccgac
     6061 gatccccttt tttaaaggtc ttcattactt gaattcggtg gttggtaaag gcccgaccga
     6121 tgtgttggct aatatgaaag cgatcggcga cgattcgggc attcggaaaa cactctttca
     6181 ccaatgtgcg atagggtgta tagaaatctg aaaccactag acgtacttgt tcacggactt
     6241 ttctagggta gcgcaagaaa tagtcgcgta aaggattgag ttgccgattt tccacaatat
     6301 caatcagttg atgggtcttg ccgtccatca tgacaaagct catggctccc gagacgtttt
     6361 tcaccgattt gaattcatcg aaacacacca cttctggtaa cggctggttc aaagaaacgg
     6421 ttttaggccg aaggctgcga agtattcgaa tgacagtggt cggagaaatg tgcttcatac
     6481 gtgcaatcaa agacatagaa ataggctcac ttaatagctc aagaatggct tgtttgactc
     6541 tgcgggcaat ggagcagcga cgatcgctaa ctgaatcttc tgctaaaaaa gtctttccac
     6601 agtgtttaca tttgaagcgc tgtttgttaa tccgcacaat ggtcttgtag ttactgacat
     6661 cattcaataa gatggatact tttttccagc cccatttaat gatcgtttgc ttattttgat
     6721 agtggcaatg gaagcattcc tctggttgat aggtcaatgt gccatcaaaa acaaaacatg
     6781 tttcgtcatt aatgcgctct ttgcgaaagg catcttcatg aaaggtaaga tttaggtcta
     6841 gaatatctag taactctttg gtaaactctg aataggacat cttgaaacac tcctttgaat
     6901 ggttttcgtc gactttattc taaaggattt caaggtgtct gtttgcgttt attagaaaaa
     6961 agaaaaagta ttggcaacag aaggcgtcac caacactaga tattatagat cctaattttg
     7021 tcgctgctcc ttttaaattt attttatcat tcggaagaac ttgtggtcgt tccgtgaagt
     7081 tcttggtacg tttgcgtttt ataaagatca acagttgatt gtccgttgtg ttcactaaag
     7141 acactagttg acttgtcacc aagagatttt tcagcttctt ttaatttctc taagccattc
     7201 ttgtagtcat aatcactggt atcaattggg tctaatccac tattcgtata gaaccgaaga
     7261 agatcaccat tattgatttg gtcagaaaca gctaactgtt tatcaacttt ttctttcagt
     7321 gaatcaattt cattctgtac ttcctcagta ggttcagtaa tagcttcacc agtttcgtta
     7381 ctatagatcg taccaccgta gtaagtatat tttggtgtaa caaaatcacc atcacggaaa
     7441 gaaacaagct gttcatgatc tttcgagaat agatcttgtc ctaactgaat ataatttttt
     7501 gtatctacac ctagtaagtg aagtaatgta ggcaaggcat caacttgtcc gccgtaagtg
     7561 tgattgattc cgccctgtgt ttgtccaggg atatggatca tataagggac acgctgcatt
     7621 tgtgcattgt cgtagtttgt ccaagtagaa cttgtttttc caactaattc agctagattt
     7681 ttatttcttg aattcgagac accgtaatgg tcaccgtaaa ggacgatgac tgaattatca
     7741 tacaagccgc tttctttcaa ataattgaag aattcttgta cggcggtatc taaataattg
     7801 gctgtcgcaa agtaaccgtt gatcgtttca tcatttgtat tggctaacgg gaatcctgct
     7861 tcgtcattgg tgaattttga ataaggataa tggttagata cagcaatgaa ttttgaataa
     7921 aatggttgtt gcagacgttc caagtattgt acggattgtt ggaaaaatgg tttatcatgc
     7981 aacccgtatt ggaacgaatt gtcatcatta acatcataat aagaagaatc aaagaaataa
     8041 tcatatccta aattcttgta tgtctcgttt cgattccaga atgtaccagc gttcccatgg
     8101 aataccgcac taatgtaacc ttgtgtttga cttaaaatat caggtgcagc ttggaaagta
     8161 ttttttccgc ctaattgtgt gaacaaggag ccttgattca agccaaacaa tgaattttcc
     8221 atcagcgttt ccgcatcgct tgtttttcct gcttttactt gatggaaaaa gttgtcaaaa
     8281 ctgaacgtgc tgttgctgtg gtaaagacta ttgatgaaag gtgtaacttc atgctggaga
     8341 ccattctcat ctgtcaattt ataatcgatc aggaattgct ggaagctttc taaatggata
     8401 taaatcacat tttttcctct agcgagcccg aacaaatcgt catttggtgc tgcataatgt
     8461 tcttttacat aattttctac ttctttcata tcattgggac ttgcttctgc ccgcacttgg
     8521 tttgtctggt aagtttgaac accgtcataa accatgaaag cattcaagcc aagatatttg
     8581 accaaataat cacgagagaa tgttcgagtc agcaattctg gacgatcagc ttccgccata
     8641 aaaagatttc ctgagaaaat cataactgct aatgtagaga tcgcaaaagc cattcttgct
     8701 cggatcgggc gagggtccaa cttgattttt tttgcaatca gcagaacagt caagataata
     8761 aaatcgatcc aatagatcac atcatatggg cgaaacagtc gaatggcact ttctcccaga
     8821 ccactcgcta ctttcccagc acctaaaatc gtgttgatcg taatgaagtc cgtgaattct
     8881 cgataataag tgacattcga aaaaacaagc aatgtcaaaa gaaaataaat gaccatcatc
     8941 gtgatataag caactctagg gcgtctgata taaagagcaa ttccaagtaa taacaatgta
     9001 gttgcaatag ggttgataaa caagataaag tattgaatgc cactttcgat tccgagatgg
     9061 aaatcaacta aataggcaaa aatcgttttc aaccaaagta aaacgactag taaagaaaaa
     9121 aatccgagcc tcttattcaa aaaggcaggt gtttttatac atttcaagat aatcttcctt
     9181 tctgcacttc ttaatcacag aagtgtcctg aaaccatgtt acacagctca cttattctca
     9241 tttattttac tagtatgaca atatagtgtc aattgaaacc agaagtttat gctgtttcat
     9301 taaggttttc tttaagcttc atgaaaaaat gagcagtaag acaagtccta tgttttttgt
     9361 tatacttggg aagagaaagc taaagcaggt gaaggaatga aagtaaaagt aaaaaaaaga
     9421 gcaagcaatc gattgaaaaa aggatatcca ctgatccaaa aagaagacct tgctcatccc
     9481 caaaaagaaa ccacgcaatg ggtggaatta gttgattcgc aaaaccaatt tatcgcaaaa
     9541 ggatatctag gaaagcagaa taagggaatc ggttgggtat tgagccagca gaacatccca
     9601 tttgatcaat ctttttttga agaatttttt gtgaagtcaa aagaaaaaag aactttcttt
     9661 ttttcagatg attctactac cgcttttcgt atattcaatg gagaaggaga cgggatcggc
     9721 ggtctgattg ttgattatta tgcaggttat gctgtttttt cttggtataa cgaaacgtta
     9781 tatgcacatc gtgaattatt gattcaagct tttcgtgaaa tctacccgga aatcatagga
     9841 ggctatgaga aaattcgatt tgaatcagta aatctgcctg agagccagtt catttacgga
     9901 aaagaggcac cggaaccctt gatcgtaaaa gaaaacggag taaaatttgc cgtttattta
     9961 aatgaaggat taatgactgg gatcttttta gaccaaaaag aagtacgggg atcattagca
    10021 gaaggtttcg cttctgggaa aacagttttg aacatgttta gttataccgg cgccttttct
    10081 gttgctgccg caatgggagg ggcaatcgaa acaacaagtg tcgatctagc aaaacgcagt
    10141 ttaaaaaaaa cacaggaaca gtttgaagtg aatggtttgg atgtcgatca gcaaaaaatc
    10201 attgttatgg acgtattcga gtatttcaaa tacgccaaaa gaaaacaatt tttatatgac
    10261 atgattgtat tggatccacc aagttttgcc agaaataaga aaaaagtctt tcgcgtagca
    10321 aaagattatg gggaactggt caaggatagt ctggatattt tagcagaaga cgggatactt
    10381 attgcgtcta caaatgctgc gaatgtctcg atagaacaat tccaaacaat gattgaagag
    10441 gaattccatg ccaaaaatgt ttcttttgaa caacttgctt tatatcgtct gccaaaagat
    10501 ttccagacga cggatacctt tttagaaggc aattatctga aagtgtttgt ttatcaaatt
    10561 aaaaataacc attagaagga gcgatgcaga atggttcggg taaagggatt gaatatagga
    10621 agcggtagac caaagatttg tgcgcctctt atcggggccg tccgagagga agtattacgt
    10681 gaagcagttc ttgcacgtga agcaggggcc gatttagtcg aatggcgagt cgatcattat
    10741 cgtgaagttt tttcttttga tgaagtagca gaaacattgg ttcttatcca tgagatgctt
    10801 gatggactgc cgttattatt tacgtttcgt accttagctg aaggcggaga acaagacata
    10861 tcgatccgtc agtatcgcga cctttatgaa catgtgatac acacaggatt agtagatatg
    10921 gtcgatatcg agttgttcaa agtagaaagt ctaggaaaaa gactgataga ggaaatcaag
    10981 tctttaaaca ttcctttaat tatcagtagt catgatttta aagaaacacc agcagaccct
    11041 gtcttgcttt accgattgaa tgttatggaa cattttggag ccgatatcgg aaaactggca
    11101 gtaactccca ataatgaacg agatgttttg cggttgatgg agttgactag acgggcaaat
    11161 gcttttttct ccatgcctat tattacgatg tccatgggaa atcttggtaa aatttctcgt
    11221 ctggttggcg gaacgacggg ttccgttatg acatttggtt ccttaagcag cgattctact
    11281 tctgctccag gacaattacc ggtcaaagca ttgaagcaaa ttattcaatt attaaatgaa
    11341 ccgttgtaga acaagtttga ggtgatattt tttggcaaag aaaaaaatag taaaaacgaa
    11401 tgcaatccgt atggtcgaac aaaaaaagat tccctataca gagcacgaat atgagtggga
    11461 cgaaagccac ctaagtgcgt catcggtcgc tgaacagctt ccagaaagcc aatctcgtat
    11521 atttaaaaca ctggttgctg tgggcaatgt gacggggcct cttgttgctg tgataccggg
    11581 agaagccgaa ttgaatctaa aaaaattagc aaaagtttca gggaataaaa aagtagaaat
    11641 gctccattta aaagatttgg aagcaacgac cggatacata cgtggtggct gctcgcctat
    11701 aggtatgaaa aaactatttc ctacttattt agatcaaatt gcagaaagct atgaacaaat
    11761 cattgtctcg gctggcagaa gagggctaca gatggagtta gcacctcaag atatctgtgc
    11821 attaacgagc ggacaatttg cagatataaa acaataaatc atacgtataa aagaaagaag
    11881 gaggccggga tgttgtttgt ccagtctcct tctttctttt ttatacatta tgctctttgc
    11941 ccggcattca ttcctgctac atgccctgta gcgaaagccg ctgtgatatt aaagccgcca
    12001 gtataaccat tgacatccag taattctccc gcaaaataca gaccatcaat gattttactc
    12061 tccaatgttt taggataaat ttcctttaga gaaacccctc cgccagttac aaatgatttc
    12121 tcaataggaa aagttttgtt gattgatagc ttaaactctt tgcatagttg aacaaaatct
    12181 tgaatttgtt tttgagaggc ttgttgtcct gtcagatgat ccatttcaag ccgctcaaga
    12241 aagaaaacta acaatcgctc aggcaatata ccatgccaag cattgaccaa gttctttttg
    12301 gttgctttac ttttttctgt caatatatga ataagctctt gtttggtttg tgtagggaaa
    12361 caatccagag aaagtgtcac tggttctccc gttttctcta attctttatt gacaaaactt
    12421 gaacaacgca gtgcagccgg accagaaata ccgaaatggg tgaaaagtaa atccatcgta
    12481 tgttcagtca atacacgacc ttttttattt aaaacgcgta aagtgatgtc ttgtaaagaa
    12541 agcccttgaa gtgttttctc ctgaatatac ggttcatccg atatcaaggg ggactctgtc
    12601 gcataaagag gcgtcaccgt gtgacccact cgcttcacga tcttgtaccc atctccagta
    12661 gaaccagtag atggataagt ccgtccacca gttgttaaga taatggcagg agcatggaac
    12721 tcttcgaatt ccgtgcgtat accgtaaatt ttatgttctt tgtggatcaa tttctctact
    12781 cgagtagaga atagaagcgt cacatctagt tctttcagac gattgagcaa tgcttcgata
    12841 atcgtttttg atttatttgt tacaggaaac atccgtccat gatcctcttc ttttaaatgt
    12901 acgccttgtg attcaaagaa gttcatgata tcgaaattat tccattgcgc aaacgtacta
    12961 tataaaaact ttccatttcc aggaatatgt gcaatcaaat catcaacagg acgattgttc
    13021 gtaacattgc accgaccacc acctgtcatc agtagttttt ttcctgcttt tttgtttttt
    13081 tcgatcaaga gtacgcgtgc gccttgttca gctgcactga tcgctgccat catcccgctt
    13141 gttccagcgc cgactacgat cacatcatac agtttttcca ttccctcacc tcaaggaaag
    13201 tgtagcatat ctgaagaaag aaaacagaaa aaaatgtttt gagccaaaag agaaaatgag
    13261 ctaaaatgag ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt
    13321 tcaagcaatt caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc
    13381 aatcgatgaa ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga
    13441 aaagaatatc ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg
    13501 gcaagatgat gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc
    13561 tatcggccct tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa
    13621 atttttggct tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg
    13681 aaaaggcgga agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg
    13741 tcaaagtttc atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg
    13801 agatatcggt gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa
    13861 aaaatatgat actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg
    13921 tacggaagct actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa
    13981 agattcattt aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc
    14041 aatcgaaaaa gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta
    14101 catctatgat ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag
    14161 acaacgtttg acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc
    14221 tgtttggaca ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat
    14281 agacgaaagc ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc
    14341 taatatgcca tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg
    14401 tccaggaaaa gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa
    14461 cgtccagcga ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga
    14521 aacgatttac catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct
    14581 tcttggagca aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt
    14641 agtttgaaaa atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa
    14701 ataataaaaa aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat
    14761 ggcacacatt cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg
    14821 gtacatgcaa agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg
    14881 aagcgacttc cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg
    14941 tatcaaagaa gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat
    15001 cggtggttct tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa
    15061 tttattagat agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag
    15121 ctcaacatat attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt
    15181 tatttcaaaa tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct
    15241 tcttgttaag aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa
    15301 agcaaaaggt gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc
    15361 tgatgacatc ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt
    15421 tagtggtgca gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc
    15481 aagcgacaaa ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta
    15541 ccgtaaaggc aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc
    15601 agaatggtgg aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc
    15661 ttcaagcgca aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg
    15721 tcgcaatatt tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc
    15781 tgaacaagca gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt
    15841 aaatacaaaa gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt
    15901 gctagtcaaa attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga
    15961 aattgctgta ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt
    16021 agaagcttac aagaaaaaca tgttcgcact tctaggcaaa ccaggctttg aagatcttgc
    16081 taaagaattg aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg
    16141 atagaaacat gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg
    16201 catcgtttat tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat
    16261 agaaatcgat tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct
    16321 gacttcgaag gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca
    16381 cttcgagtgc ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca
    16441 gatgagggta ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct
    16501 ttcaactgca agatcgttcc ttcggggtaa gcagaaccat tggcagagtc accagaagta
    16561 atgacatcga aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat
    16621 ttaaaacctc ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt
    16681 ggagctttga ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa
    16741 cctttccatc ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg
    16801 aaagatctcc ttttttattt ttattatacc cgtggtgcta gttaatttta atatcctaaa
    16861 gaatatctca aagttccttc aaaacgt
//
LOCUS       DACEQH010000057        16591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.058, whole genome shotgun sequence.
ACCESSION   DACEQH010000057 DACEQH010000000
VERSION     DACEQH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.058"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(45..779)
                     /locus_tag="IW833_002092"
     CDS             complement(45..779)
                     /locus_tag="IW833_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1308191.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRFVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1105..1515
                     /locus_tag="IW833_002093"
     CDS             1105..1515
                     /locus_tag="IW833_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ1308192.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     EGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            1508..2209
                     /locus_tag="IW833_002094"
     CDS             1508..2209
                     /locus_tag="IW833_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ1308193.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2209..3822
                     /locus_tag="IW833_002095"
     CDS             2209..3822
                     /locus_tag="IW833_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1308194.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            3812..4033
                     /locus_tag="IW833_002096"
     CDS             3812..4033
                     /locus_tag="IW833_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ1308195.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4030..4791
                     /locus_tag="IW833_002097"
     CDS             4030..4791
                     /locus_tag="IW833_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ1308196.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5172..5681
                     /locus_tag="IW833_002098"
     CDS             5172..5681
                     /locus_tag="IW833_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1308197.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5751..6290)
                     /locus_tag="IW833_002099"
     CDS             complement(5751..6290)
                     /locus_tag="IW833_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ1308198.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVVVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6430..7041)
                     /gene="rpsD"
                     /locus_tag="IW833_002100"
     CDS             complement(6430..7041)
                     /gene="rpsD"
                     /locus_tag="IW833_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ1308199.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7357..8154
                     /locus_tag="IW833_002101"
     CDS             7357..8154
                     /locus_tag="IW833_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ1308200.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPAATILV
                     EGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLGYEHVIANFPAFTLAYFAS
                     QGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8182..8817
                     /locus_tag="IW833_002102"
     CDS             8182..8817
                     /locus_tag="IW833_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ1308201.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8837..9610
                     /locus_tag="IW833_002103"
     CDS             8837..9610
                     /locus_tag="IW833_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1308202.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10018..10815
                     /locus_tag="IW833_002104"
     CDS             10018..10815
                     /locus_tag="IW833_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ1308203.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKKIQLIQINDDRPFME"
     gene            10793..11206
                     /locus_tag="IW833_002105"
     CDS             10793..11206
                     /locus_tag="IW833_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308204.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11190..13835
                     /gene="mutS"
                     /locus_tag="IW833_002106"
     CDS             11190..13835
                     /gene="mutS"
                     /locus_tag="IW833_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1308205.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLKKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKNSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13853..15961
                     /gene="mutL"
                     /locus_tag="IW833_002107"
     CDS             13853..15961
                     /gene="mutL"
                     /locus_tag="IW833_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ1308206.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPVV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15983..16543
                     /locus_tag="IW833_002108"
     CDS             15983..16543
                     /locus_tag="IW833_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ1308207.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARIVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
BASE COUNT         5387 a         2964 c         3598 g         4642 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaaaacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc gagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctaa ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 ttcacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaagaaac atcaacacga aaagcatcgg
      841 tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata aaaaaatgaa
      901 ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt aaaagttctc
      961 cacaaaccac caagcttaac tataaatata ccaaaaaaaa aacgtttttt ttctcaaaaa
     1021 ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag taaaatgaaa
     1081 gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat tattattttc
     1141 ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc tggaagtatc
     1201 ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc tgaaaaaatc
     1261 ggagcaactg gtgatttttt attgaataat ctagctattt tatttgttcc tgctggtgta
     1321 ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact gggagcagtc
     1381 gtcgtgtgtt ccatcgtcac gatggttgca gagggaaaaa cggcagaagg tgtagaagct
     1441 ttgctgggct atgtccgtaa gaaaaaagca ggaagggtat ctgtaaacga aatggaggaa
     1501 aatcaaattg actgagtttt ttgtgaatcc actatttggc ttaagtttga ctttaggagt
     1561 gtatgtatta tctgataaat ttttgcggaa atataaactg cctgttttga atcctctcgt
     1621 attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct ataaagacta
     1681 taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg cacttgccat
     1741 ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa tggctgcgat
     1801 tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact tcttctctct
     1861 tcataaagag atggtgatct cattactacc taaatctgta acgacggcta tttctgtcga
     1921 tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg tctctacagg
     1981 tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg aaagtccagt
     2041 tgctcgcggt gtggcgttag gaagtacttc tcatgctatc ggtacagcaa aagcaatcga
     2101 gattggcgaa atagaaggaa tcatttccgg acttgcgata tgcgtaaatg ggatattgac
     2161 cgttttgttg ctgccgttat tctttcagcc ttttgctggc ttgttttgat gcgaaaacca
     2221 ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa agacgtgtat
     2281 ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat tctcctccta
     2341 gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt tgttcagctg
     2401 gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca gcagtttagc
     2461 ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt ttatgatacg
     2521 ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac gctgaaattc
     2581 cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt tgcgcaaaag
     2641 cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca gcaactgcaa
     2701 acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga agaaatcatt
     2761 cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg gccatttgaa
     2821 acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt ttttaatgtt
     2881 cgtctgaacg agatcaaaaa aagacgattg gaatacatgc taggtgcctt ttttttaaga
     2941 aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat ttctgacaat
     3001 ctgttgttcc agcgattttg ccaagtcatg gagccggtat ttcctaatta ttttcaagta
     3061 gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata ttatagtgat
     3121 cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc gccctttaca
     3181 gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg gctgccagca
     3241 gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt cgcctatctt
     3301 tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa tcatttagtt
     3361 attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag acacaaacat
     3421 cccaatcatc tggcatttaa aaatcatgcg tttttgatgg gtcggtatct gactgtgttt
     3481 aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt aatgacagat
     3541 ttcccattat ttgaagagca actgttagag gagggattac ggacattttt ccgtaatgag
     3601 tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct tctgatttcg
     3661 acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt gaccgaagaa
     3721 ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca aaaaaaaact
     3781 attaaaacaa taacaaagaa ggagaaaaaa gatgacattt gaagaaattt taccagaatt
     3841 gaaaaaaggt gcaaagatta ttcgtaaagg atggagtgga tttgaacttt atgttgttct
     3901 cgtatccgat gatgaatttg atggctgtcc agtgacacct tacttattga tcaaaacaag
     3961 tgacgaaggt ttttccagct tcgctcctac agtttgtgat attttagcag atgattgggc
     4021 gatcgtagaa tgaaaaacca gtatccagat ctagctaaac aggttgtatt tatcacagga
     4081 gctgcttctg gaattggtgc tgcgcaagcc cgagcgtttc ttgaacaaga tgcttttgta
     4141 tttggattag atcaaaagtt cggtaaaatg gaagaactct ctttgcgatt tcctgaaact
     4201 tttgcctacg cactaggtga tgttcgacaa atgaaagaac tccaacaagc ggtatcgaaa
     4261 tgccaggatc gttttggtga agttacgatt ttactgaata catcggggat attggatgcc
     4321 tacaaaccat tgatggaaac tgacgaatcg ctgtgggatc ttatttatga gacgaatgtg
     4381 aaaagcatgt atcagctgac aaaaatcatc cttcctgata tgatccgccg aaaaagcgga
     4441 acaatcatca acatggcttc gattgcgggt ttggttgctg gcggaggtgg gatcgcttat
     4501 acaagtgcga aacatgcaat cgtcggattt accaaacaac tcgctctgga ttacgcagga
     4561 cagggtatcc gagtaaaagg aattgctcca ggagccatcc aaacaccaat gaatgcggct
     4621 gattttgaag ggaaaggtga gatggctgag tgggtcgcaa atgaaacacc agtcaagcga
     4681 tgggcacagc cggaagaagt agctgagctg acgttatttc ttgcctctcc tcaagcaagc
     4741 tacatacaag gaacgatcat cccaatcgat ggcggatggt tgttaaaata accttgtgtt
     4801 tttttcaagc ttctgtatga taaaactaaa gagagaaaca acggttgatg ataaaagaaa
     4861 gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt ctttttaata
     4921 atcgttaata ttcgaaacta agcggctttt cacgaccttt tgatataaac ggtgttcaaa
     4981 agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct atttttattt
     5041 gactattctt attacttgtt gcagggcagc tttttcacta ccttctgcgg cacttcagtt
     5101 ggaattttct tcccatcctt tggtcgcagt cattaccaag ataaaattat cttgcaaagg
     5161 agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca acgacagatg
     5221 tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt ttgtctgtct
     5281 ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg tccttgtata
     5341 acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatg gcttctcctc
     5401 ttggactgat agatgttgta gtaggaagcg tcagcacgct gcttgtattg cttatcaact
     5461 ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggctgtgaca gcatgcgtat
     5521 ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa ctaccttttt
     5581 tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc ggtgggataa
     5641 ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt taatttaaaa
     5701 tatggcagcg tggaagagac tttgagtctt cttccatgct gcttttttga ttaatttgta
     5761 tgaagaaatc gatttagccg ttttttcatc aagataccga tataagctgc tccagttgcc
     5821 ttgattgctt ccggcagtaa aaacggcagt agtccactgg caaatgcagt tgtaaaagta
     5881 agatcaccac ttattttcag ccagatcgtt ccgcagatca atgcagcaag agagcccatt
     5941 agattggcaa agattgcata taagtaatga gcatccgttc tttcgatcat ccaaccagtt
     6001 atccacgttt gtaagagaaa tccaatcaaa tatccgccag ttggcccaaa taaaacagct
     6061 atacctccag tcatccctga aaatacagga agaccaatca agccaagtaa aagataaact
     6121 acaacagata tcaatcctag ttttcttccg agaaaagttg ctgttaatcc aatagcaaat
     6181 gtttgccccg tcaaaggaac caagccaaaa ggaatggtca tctgagaaag gacagaaata
     6241 atcgcggcaa attccgcagc aagtattaat tctttcaatg ttcggttcat aatgccctct
     6301 tttcgttaac ttaatatttt tatttggtta acgaaattgt aaaacaaagt gattactttg
     6361 taaatagaaa atataaaaaa agaccagcta tccaatagat agcggtcttt ttcaagaaag
     6421 cttttgaaat tataagcttt ggttgtagta ttcaacgaca agagcttcgt cgatttctgg
     6481 atatagttcg tcacgttctg gtaaacgagt caatgaacct tccaattttt cagcgtcaaa
     6541 gcttacgaat gctgggcgtc caacagttgc ttctacagct tcttttactg ttacgatgtt
     6601 ttgtgatttt tcacgaactg agatcacttg acccacttct acgtggtatg aagggatatc
     6661 aacgcgtttg ccatcaacta aaatgtgacc atggttaact aattgacgtg cttgacgacg
     6721 agtcgttgca agtcctaaac ggtaaacaac attgtctaaa cgttgttcta gtaagatcat
     6781 gaagttaaca ccatgtttac cttctttgat tttgctagct tttacgaaca agttacggaa
     6841 ttgacgttcg ttcattccgt acatgtgacg aagtttttgt ttttcagtta attgcatgcc
     6901 gtattctgat tgtttgccac ggctgttagg tccatgttga cctggtgcgt aagggcgacg
     6961 tgctaattct tttcctgtgc ctgacaatga gatgcctaag cgacgagaaa ttttccatga
     7021 tggtcctgta taacgagaca ttaaaatttc ctccaatgaa tataagtttt tggagtaaaa
     7081 taatccgttg aaaaattcat aatcatatag tttgttcttc aatcttcacc tttgcagccg
     7141 cggttacaca atcgaacccg agacggggca acaaactagg gatgagttat tatttttctg
     7201 ctgcattatt ttacacaaac tacagtatat gatagatcat ccacgaatgt caagataatt
     7261 agtacataaa aacgctttac aaacctaggc tgtctttata aaatgagagg gcatgtgcaa
     7321 atggaaaaaa gcgaaggaag tttaaggaga aaatcaatga atccggtatc accattattt
     7381 gagaaaatcg atgcttcaat tgaaaagaag gcgaatcttt ttgaaaacag cttttcacgt
     7441 tacgctgttc gagcggttct cgcctgtttg tttttgactt taggaacggc tgtggcattt
     7501 gcaatcgcga tgaaggggga agatatcgcg catggtctag gaaagatttt atacgcattt
     7561 atgttcagct ggggtctggt catgattcta tacatgaacg ctgaattagg aacatcgaat
     7621 atgctttata tgaccgtggg tgtttttcgg cagagacttg ctttaagtaa ggcagccaag
     7681 attctatttg cctgtatttt tttcaatatg attggcggta tattatgtgg atttcttatt
     7741 tctatgacta cgccatttca agatttaacg ttggacaact ttatgttaga atcaatagca
     7801 gtaaaactag caaaacctgc agcaacgatt ttggtggaag ggatgtttgc gaatatcgtt
     7861 gtcaataccg ctgtactgat cagcatgcgg atgaaagagg atgccgggaa agtgatcaca
     7921 gtcgtattca tcattttcat ttttgctttt ttaggatatg aacacgtgat agcgaatttt
     7981 cctgccttta cgcttgctta ctttgcttct caaggacaga cggatggaat gacagtcagc
     8041 aatgtgttac ataatttgtt ttttgctttg gcaggaaatt atattggtgg cggtttagta
     8101 atgggactgg gatatgcttg gctgaatcag tcaaaatcat cctatgtgga ctaaaaagtc
     8161 gttcattaaa ggggaagtgc catggaagtt ttagtttttc caaaaaatga ggaaaaagcg
     8221 ttacaaatgg aaaagtacat gaaaaatctt ttttcctttg ctggagtacc taagccggaa
     8281 cgcgctcttt tacaacaacc gttactggat gagagcaaaa atttatctat ttctgaagtt
     8341 ttagcttttg tttattatta ttatgaacaa atcgaaagag agtatcagta tgtagcgatc
     8401 gatttagtcc agaaaaacat caaacggctt acatttgaag acttagtcgt attgttgcca
     8461 ctagttcaga aaaaagaatg gtgggacagc atcgactctt ggagaaaagt atacagcaca
     8521 tggtgtaaag cctacccaga gcagcgtgaa gaagtctttc aattgtttga aaaaaaggaa
     8581 gatttttggc tacgtcgaat agcaatcacg ttgcaattag gttataaaca gcaaacagac
     8641 acaggactat tgatccgagc aatagacgaa gatcgtcaaa caaaggaatt ctttatccaa
     8701 aaagcaattg gttgggcatt acgcgattac agtaagaccg atgccgattg ggtaaaaaga
     8761 cagctgacaa cagatttgag taagttagct gttcgagagg caagtaagta tttataagaa
     8821 agcgagacga caagagatga gacgcaaatt atttgctttt gatattgatg gaacgttgtt
     8881 agattcaaac aaacaagctt tagatagcac gagagaagca ttggaaaaat tacgcaaagc
     8941 cggtcatctg gtcacgattg ctacagggcg gagccgattt catgcgcaag agatcattcg
     9001 cgatcttgat tttacgaact acattttatg taatggagcg gccggttttt tagaccatga
     9061 acaagtatat aagaatttac tagatcgcga ccagttggat gcgttcgttc aagaagcgca
     9121 tcatgagcag atcgatacag cttttgtcag cttagacaat atcaagcgtt cttcttccaa
     9181 tgatgtaacg gtgatggaag aagcgatgca ttctttcggt gctgtcttgc ctgaattaga
     9241 tcagtttttt gcagaggaac aagaaatata ccaagcgtta gcatttttcg atcaaacata
     9301 tgatgggaag ttctcttcat atgataaaat tcgatttgtc cgctggcatg agaacagtgt
     9361 agatgtcgta cctaaaaatg gttctaaagc agcgacaatt ctgcacttag cagaacaagt
     9421 aggcatccct catgaagaca tcatcagctt tggcgatgga caaaacgacc gtgaaatgtt
     9481 gcgcatgtca ggaattggga tagctatggg gaatgctgtg ccagaagtac aagcagaagc
     9541 taagatggtc actgatacaa acgatcagga tggaatttgg aaggcattga aagagttaag
     9601 cgtgatttaa aagaggttgt gaaaaagctg ttctgcatca agtaataagc ccagccaaac
     9661 aaaaatagct cctcctattt ttcgtttttc tgggcttatt accgcagatg caagcttttg
     9721 aacaccgttt aaggaagagg tcgtgacaga agtggtcagt ttcaagaaat aagaagggat
     9781 ttacgaaaat tgcttctcaa atttttgtaa aattacggct tatttctgaa gaagtagatg
     9841 aaacaacgtt tattcgggaa atgggactgt gacaagatgt ttgtcatagt cccatttttt
     9901 tattttattt ttcaaaacta agaaatacca ggaaagagtt ccttttgttt gaccttttct
     9961 ttcttattga aaacaactat aatatagagt gatgttaaaa gagaagagga atttagtgtg
    10021 cgggtattgt ttattggtga tgtagtaggt tcaatgggac gagagatgat tactgaatat
    10081 cttcctagat tgaagaagaa gtaccgtccg caagtaacga ttgtaaatgg tgagaatgcc
    10141 gcttctggac gtggaatcac agaaaaaatc tataaaaaat ttttgcaaga cggcgtagat
    10201 gtcgtgacga tggggaatca tacgtgggac aaccgcggaa tctttgagtt tgtaaatgaa
    10261 gctaaaaaga tggttcgtcc ggcaaatttt ccggaaggga cgcctggaca agggatggtc
    10321 tttgtcaaag tgaaccaagt cgaactggca gtgatcaaca tgcaggctcg ttcttttatg
    10381 gtggacttgg atgatccttt tcgtaaaatg aatgagctgg tagaagaagc cagaaaacgt
    10441 acgccgatca tttttgtaga ttttcatgga gaaacaacaa gtgaaaaaca agcaatgggc
    10501 tggtttttag atgggaaagt ttcagcagtg gtcggtacac atacacacgt tcagacaaat
    10561 gatgcgcgaa ttttgccaag aggaacagcg tatctgacag acgttgggat gacaggtccc
    10621 tatgatggta ttttgggtat gcgcagagaa cctgtaatcg aaaagtttct gaccgcatta
    10681 ccaaaacgtt ttgaagttgt ggaacaaggc cgatcgattt taagcggctg tatcttggaa
    10741 ttggatgata cgacaggtca tgcaaaaaaa atccagctga ttcagatcaa cgatgatcga
    10801 ccgttcatgg agtagaaaga gaggcagttt tttgcagcag gagccggaaa ttcaaaaaga
    10861 agtaaggaaa ctgacaaaac ttttaagaga gaatgaaacg atcattcgct acaaagaact
    10921 tgaagaaaag atccagcaaa accagtattt agctgagttg agagaaaaaa tcaaacaagc
    10981 gcaaaaagat gctgttcatt ttgcccacta tgacaaacca gcagcagaac aagaagcaat
    11041 caaacaagcg gatcaattca tgcaagaatt tgatcagcat ccgcttgttg tagcttatag
    11101 aaaacagctg ttggaagcag atgatctgct tcatcattta acaactatga tacaagaaga
    11161 aatcaatgga caaatagagg aggaaaaaca tgcctcaaaa aactaaaaat actcctatga
    11221 tggaacaata tctaagcatc aaggcccaat accaagatgc ttttttgttc tatcgtttag
    11281 gagactttta tgaactattt tacgaagatg cgatcaaagc gtcacagctt ttggaattaa
    11341 cgttgacgag cagaaatcga aatgcagaag aacctattcc tatgtgcggt gtacctcacc
    11401 atgcagcaca aggatatatc gatgccttga ttgaaaaagg ctataaagta gcgatttgcg
    11461 agcaggtaga agatccaaag acgactaaag ggatggtcaa gcgcgaagtg atccagctag
    11521 tcactcctgg tacagtcatg gacagcaaag gattgtcggc aaaagacaac aattttttaa
    11581 cagctgtagt tagtcaagga aatgaatttg gctttgctta tgccgattta agtacgggag
    11641 aattgaagac ggctcggttg catgatgaag aagctgtttt aaatgaagcc tctgcattgc
    11701 agacaaaaga actcgtatta ggcagtgaga tcactgatac ccttcgtgaa cagctaagcg
    11761 ggcgtttagg gctagtcttt tctcaacaaa atgagatgga agaaaacgcg gaatttagct
    11821 ttttgacaag cgaactagtc gatccgctga aaaaagaagc aaccggcaaa ctattgactt
    11881 atttatcggt gacacaaaag cggagtttag ctcatatcca aaaagcagta gaataccaac
    11941 cagatcattt tttgaaaatg gattattatt caaaattcaa cctagaatta agccaatcta
    12001 ttcggactgg gaaaaagcac ggcactcttc tatggttgtt agatgaaaca aaaacagcaa
    12061 tgggtggccg tttactaaaa caatggctag accgtccgct tatccaggca aaacaaatta
    12121 aagcaagaca agaaatggtc gcttctttgt tagacagcta ttttgaacgt atcgatttgc
    12181 agtcagcatt aacgaaagtc tatgatctgg aacgcctagc aggtcgagta gctttcggta
    12241 atgtcaatgg gcgagacttg atccagctga aaaattcttt agaacaagtg ccaatgatca
    12301 gagggttgat tgaaggaatc gatcaaggtc aatggcaaag tctgctttca gaaatgcagc
    12361 cgatggacca tctcgtccaa ttgatcgacc aagctatcca agatgatccg ccgttacaaa
    12421 taacggaagg aaacatcatc aaagatggat tcaatgagca gctcgatatg taccgttctg
    12481 ctatgagaaa cggaaagaaa tggttggcag aacttgaagc aaaagagcgt caagaaacag
    12541 gaatcaaaaa cttaaaaatc ggatttaacc gcgttttcgg ttattatata gaaatcacaa
    12601 aagcaaatct tggaaatgca gaactggaga agtatgaacg aaaacaaact ttagcaaatg
    12661 cagagcgttt catcacacca gaactaaaag aattggagac acaaatcctc gaagcagaag
    12721 aaaaatctgt tgatttagaa tatcagttgt ttttagctgt gagagaagaa gtcaaaaagg
    12781 ctatccagcc gctacaagta cttgcaaaag caatcagtgc agtagatgtg ctacaaagtt
    12841 ttgcaacgat cagcgaacgc tatcagtatg ttcggccaga acttgtcagt gacaagcatc
    12901 agcttctgat caaagacgga cgacatcctg tagtagaaaa agtattagga catcaggaat
    12961 atatccctaa cagcgtagaa atggcagaag acgaaatgat cttgctgatt acaggaccga
    13021 atatgtctgg taaaagtacg tatatgcgac agttggcatt aactgttctg atggcgcaaa
    13081 tgggctgttt cgttccggca aaacaagcag ttttacctat ttttgatcgg atttttaccc
    13141 gaattggtgc aagtgacgac ttaattgctg gacaaagtac ttttatggta gagatgatgg
    13201 aagccaatca agccctgcgt tatgctacgc caaacagttt gattttgttt gacgaactag
    13261 ggcgaggaac agcaacctat gacggaatgg cgttggccca agcaatcatc gaatatatcc
    13321 atcgtcatgt acaagcaaaa acactctttt ctactcatta tcatgaactg acggtacttg
    13381 agaaagaact tccgcagctt aaaaatgtcc atgttggagc cgttgaaaaa gacggagaag
    13441 ttgtcttctt gcataaaatg atggatggac ctgcggataa aagctacggc atacatgtgg
    13501 caaagatcgc aggattgcct tccaatctac tagaacgagc agcagatatc ctgcatacgc
    13561 tagaaaatgg cgataacgga caccatactg ttccagctga agtagaagag aaaactaaaa
    13621 cagccaaaca ggagcaaata aaaccacctg cagaaaaagt aaatcaagac catatacaag
    13681 accgtgtaaa agaagaaaac caacagcttt ctttattcag tgaattatcc gaaaatgaaa
    13741 cagaagtcat tgaatcactg aagcaaataa atttactaga aatgacgcca atggatgcac
    13801 taaacacatt atatgaatta caaaaacaac tgtaggaaga aggcggatat cgatggccaa
    13861 aatccaagaa ttatctgaac gtttagctaa tcagatcgct gcgggagaag tagtggagcg
    13921 accagcttcc gttgtgaaag aattagtgga aaatgcaatt gacgccggca gtactcagat
    13981 tgatatctta gttgaagaag cgggactgcg gacgatccaa gtgattgata acggagaagg
    14041 cattttggca gacgacgtag agaatgcctt caagcggcat gcgacaagta agatccataa
    14101 tcgcgacgat ctttttcgca tacgaacgct gggctttcga ggggaagctt tgccaagtat
    14161 tgcctctgtt tctgaaatga ctctggaaac ggcgaatcag gaagaaaaac aaggcaccta
    14221 tctgtcgctg aaaggcggag aagttattga gcatcgacca gcaccactgc gtcaaggaac
    14281 gaagattacc gtcagcaatc tgtttttcaa tacgccagcc cgcttgaaat acgtgaaaac
    14341 actgcacaca gaattagcga acatcgggga catcgtcaat cgactggcat tgagtcatcc
    14401 atcgattgct tttcgtttag tacatgatgg aaacaaaatg ctggcaactg ctggaaatgg
    14461 cgatctgaaa caaacaattg cgggaatcta cggattatct accgcaaaaa aaatgctgaa
    14521 aatcgaagga aaagatctag attttgagtt atttggctat gtttcattgc ctgaggtgac
    14581 tcgtgccagt aggaattact tatcgacgat catcaatgga cgttttatca agaacttttc
    14641 cttgaataaa gcaatcgttg caggttatgg gtcaaaatta atggtcggtc gtttccctat
    14701 tgctgtattg gaaatcaaaa tggacccgct tttagtagat gtaaatgttc atccgacgaa
    14761 gcaggaagtc cggctgtcga aggaaaaaga actaacagag ctaattagta acgccattca
    14821 aaaagcattg tctcaagaaa atctgattcc aagtgcagcg gataatctac ggtttaagaa
    14881 aaaagtacct gatcagccta aaacagaaca aatcgaaatc gatttgtcct acgcttttga
    14941 agaagaaaaa cctaaaaagc caagtagttt gaattttgat cgagagacag gtaccttttt
    15001 cgtagaagaa ccaaagaaca catttgttga cagaattgtt gataagtctg tggaaaatgt
    15061 tgaaaactat tctggttttt ctgaagaaaa caccacttat ccagaaaaaa cagcgaaaac
    15121 ggtggataag tttggtgaaa atgttgataa ctttgtagaa aaaacagtgg aaaaatctga
    15181 aaagaaagaa tttcccgttg tggataacat ggagaaaaca aatgaagaac aagcagataa
    15241 atccgtagaa gaggatgaaa agatttacga ggaagaattg acactccatc ctgaatttga
    15301 tttcactcaa ggaaacgaca aaacattaga acaagccatc aagaaattag aaacggaaaa
    15361 accagtacaa cgttttcctc agttggaata ttttggacag atgcacggaa cgtatctttt
    15421 tgcccaaagt agcgatgggt tgtacatcat tgatcagcat gctgcccaag agcgcattaa
    15481 gtatgaatat ttccgggaaa aaattgggga agtatctgat gatctgcaag aattgcttgt
    15541 tccatttgtg ttagactatc caaacagtga tgctttgaaa ttaaaagagc aaaaacactt
    15601 gctagaagat gcagggattt atttagaaga atttggtcaa aacagcttta ttgtacgggc
    15661 ccatcctact tggtatccca atggagaaga agaatcgatt attcgggaga tgatcgatat
    15721 gctgctgaca actggaagtg tgagtgtaaa aaaattccgt gaagcaacag caatcatgat
    15781 gagttgcaaa cgatcgatta aagccaacca ttatttgaat gaagcacaag cacgtgtgct
    15841 gctgaaggat ctggcacgat gcgaaaatcc tttcaattgt ccccatggcc gtccagtatt
    15901 gattcacttt accaattcgg atatggaaag gatgttcaaa cgaatccaag acccgcatta
    15961 agtcaaagag aagagggaaa acatgaagat tgtattagca tcccaatcac caagaagaaa
    16021 agagttattg gctcatatcg tttcagaatt cgagattgaa cctgctgata tcgatgaaac
    16081 gccatttcca gacgaacagc ccgtcgatta tgttcgtaga atggctgaag aaaaagcacg
    16141 aattgtctgg gaacaagatg aaaaagaaga ccatttagtg attgccagtg acacgactgt
    16201 tgttctcaat caagagatca tgggtaaacc agaagatctg gctgaggctg aattcatgct
    16261 gaaaaaacta agcggggaaa cacatactgt atatacagct gttgttttaa aaacaaagga
    16321 acgagaagaa cggatactag cagaagcaca tgtcactttt tatcctttga cagacgaaga
    16381 gatcaaacgc tacttggaga cgggggatta tgcggataag gcaggggcat acggcatcca
    16441 aaatgctgcg agtgtattcg tcaaagaaat ttctggagat tattacagta tcgtaggatt
    16501 tccgattggt gctgtcaatc aggcactgaa aacattcatt taaacaaaaa agcggactat
    16561 ctgattttcg attgaaaatc catgatagcc c
//
LOCUS       DACEQH010000058        16528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.059, whole genome shotgun sequence.
ACCESSION   DACEQH010000058 DACEQH010000000
VERSION     DACEQH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.059"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(141..416)
                     /locus_tag="IW833_002109"
     CDS             complement(141..416)
                     /locus_tag="IW833_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ1308208.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(659..1969)
                     /locus_tag="IW833_002110"
     CDS             complement(659..1969)
                     /locus_tag="IW833_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ1308209.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="IW833_002111"
     CDS             complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="IW833_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ1308210.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3515..4195)
                     /locus_tag="IW833_002112"
     CDS             complement(3515..4195)
                     /locus_tag="IW833_002112"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ1308211.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4244..4879)
                     /locus_tag="IW833_002113"
     CDS             complement(4244..4879)
                     /locus_tag="IW833_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1308212.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4945..6348)
                     /locus_tag="IW833_002114"
     CDS             complement(4945..6348)
                     /locus_tag="IW833_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ1308213.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6335..7366)
                     /locus_tag="IW833_002115"
     CDS             complement(6335..7366)
                     /locus_tag="IW833_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308214.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7397..7618
                     /locus_tag="IW833_002116"
     CDS             7397..7618
                     /locus_tag="IW833_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ1308215.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7783..8520)
                     /locus_tag="IW833_002117"
     CDS             complement(7783..8520)
                     /locus_tag="IW833_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ1308216.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8673..10013)
                     /locus_tag="IW833_002118"
     CDS             complement(8673..10013)
                     /locus_tag="IW833_002118"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ1308217.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10250..11773)
                     /locus_tag="IW833_002119"
     CDS             complement(10250..11773)
                     /locus_tag="IW833_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ1308218.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12151..12762)
                     /locus_tag="IW833_002120"
     CDS             complement(12151..12762)
                     /locus_tag="IW833_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1308219.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12885..13010)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13092..13808)
                     /locus_tag="IW833_002121"
     CDS             complement(13092..13808)
                     /locus_tag="IW833_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1308220.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13814..14383)
                     /gene="scpB"
                     /locus_tag="IW833_002122"
     CDS             complement(13814..14383)
                     /gene="scpB"
                     /locus_tag="IW833_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ1308221.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14367..15164)
                     /locus_tag="IW833_002123"
     CDS             complement(14367..15164)
                     /locus_tag="IW833_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ1308222.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15134..15505)
                     /locus_tag="IW833_002124"
     CDS             complement(15134..15505)
                     /locus_tag="IW833_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1308223.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15524..16411)
                     /gene="xerD"
                     /locus_tag="IW833_002125"
     CDS             complement(15524..16411)
                     /gene="xerD"
                     /locus_tag="IW833_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ1308224.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4645 a         3577 c         2798 g         5508 t
ORIGIN      
        1 aaaaattaaa taaaatacat gtgttttcta tgtatgacat acggattcgg aatctctgtc
       61 tcttatttat tccttttctg ctaggaaata aaaagaaaag cacttgtagc cctagagcca
      121 caagcgcttt ttcaactgtc ttatttaaca gcgtctttta atgctttacc tggtttgaat
      181 gcaggtactt tgctagctgg aatttcgatt tcttcaccag tttgtgggtt acgacctttg
      241 cgagctgcgc gttcgcgaac ttcaaagtta ccaaaaccga ttaattgaac tttttcgccg
      301 ttagctaaag catcttgaat agttgaaaat acagcatcta ctgctgcagt tgcatctttt
      361 ttagttaaat cagtagctga agcaactttt tcgattaatt ctgctttgtt tgccatggat
      421 agattcacct cctccacaag agatatgata gaaatatcat ttagttaaaa ctttctgagt
      481 ggtccagaag tttaaaaaga aatattgatt aacttcaata ttctgttatc acgatatcat
      541 atgaaccgct taatagcaag gtttttacca attttcacat gcttttttta gtcatagaaa
      601 cgtaacaaac gaaactgtct ttttcttttg aaaaaaaaac gtcgatatgg taaaatgcct
      661 attttctcct acgagggata attcggatgg acgtcccttc aaaagtaaat gcttttctga
      721 tttgattttc taagaaacgt tcataagaaa aatgcatcaa ttcctcttca ttgacaaaaa
      781 cgacaaatgt aggtggtttg acagcgactt gtgtagcata aaaaatcttc aggcgttttc
      841 ctttatctgt tggtgttgga ttgattgcta cagcatccat aatgatatca ttcaacacag
      901 ccgaagagat acgaagattc tggttcatac ttacttgttc gatcatttca ggcagacgtt
      961 ccaaccgttg tttagtcact gcagaaacaa aaacgattgg agcgtaatca agataccgga
     1021 attcatcacg aatctcttgt tcgaaatcac gcattgtatt ggtttctttc tttacaagat
     1081 cccatttatt cacgacgata ataattccac gaccagcttc atgggcgtaa cccgctactc
     1141 gcttgtcttg ttcccgaatc ccttcttcag cattcagtac catcaaaacg atatcagaac
     1201 ggtcaatggc acgcattgca cgcatcacgc tgtatttttc agtattttcg tatacttttc
     1261 cgcgtttacg cataccagct gtatctatca ttaagaattt ctgaccactt tcagaaacga
     1321 agtgggtgtc gattgcgtct cgggtcgttc cttcaatatc cgaaacaatt acacgttctt
     1381 ctcctaaaat cgcattgatc aaagaggatt taccaacatt tgggcgacca atcaaactaa
     1441 acttaatcgt gtcgtcgtcc tcttcttcag tctcttcaga gaaatacttc actgcttcat
     1501 ctaaaatatc accaatccct agtccatggc ttcctgaaac aggataagga tcgcctagcc
     1561 ctaaggaata aaattcatag atatcgtttc tcatttctgg attatctact ttattgactg
     1621 ctaaaatcac tggtttatta cttctgtata aaattttcgc aaccatctca tctgcgtctg
     1681 tcacgccttc gcgtccgctg actacacaaa tgattacatc tgcttcttca atcgcgattt
     1741 ctgcttgatg cttgatctga tccataaaag gttcatcgcc aagatcaatt ccgcctgtgt
     1801 cgatcacgct gaattctcga cctaaccatt caccttttgc ataaatacga tcacgagtta
     1861 ctccaggagt atcttcgaca atcgagatac gctcaccagc gatgcggtta aaaatcgtag
     1921 acttaccgac gttcgggcgg ccaacgattg caattgttgg atttgccata ttatttcctc
     1981 ctcacaagtt tccaatctca cttagtttaa cgaatgcatt gccaactttc aagcatagat
     2041 tgtcaattaa acgaagtatt tttattcctt attgaaatac taatcgcttt tcaaaagaaa
     2101 aactgagaca aaagcagctg ctttcatctc agtcacagat acaaaatcaa ttatttttca
     2161 gaatcatcgg ttgctttttc ttctacttct tctttcaacg catcacctaa aatgtctccc
     2221 atagtaaatc cagtattttc ttctggtaat tcataagact caacattttt tggttcttct
     2281 tctgaagctg gtttttcttc taaagctttg atactcaatg caatacgatg ttcttctgga
     2341 tggatctcta atactttcac tttcacttcg tcaccttcat gaagcacttc atgtggtgtc
     2401 gcaatatgtt tatgtgaaat ttgagagata tggactagcc cttctactcc agggaacact
     2461 tcaacaaagg caccgaagct tgtcaaacgt ttaactgttc cgtctaatac tgtgccaact
     2521 ggagcttttg tatcgatttc tgaccaaggt ccaggctgtg tgtctttgat tgacaaggaa
     2581 acacgttctt gttcaggatt gatagacaac actttgacat ttacatcatc attgacagcc
     2641 aatacatcac ttggttttgc tacatgacta tgtgaaatct ctgagacatg gactagaccg
     2701 tcaattccgc ctaggtcgac aaatgcgcca aaatcagtaa gacgtgcaac acgtccactg
     2761 agaacatctc cctcatggat agttgaaaga atttcttttt tcttttcttc tctttcgttt
     2821 tccactaccg ctttatgcga taagatcaaa cgattttctg atggttcgat ttcgatgatt
     2881 ttaaagctca atgttttgcc tttatagtct gaaaagtctg caacaaaatg atcttccacc
     2941 attgatgcag ggacaaaacc acgtacaccg acgtcaacga ctaaaccgcc tttgacaaca
     3001 tttgtaacag gtgcttcgat gaccttgccc tcttggaaat ctctttcgat atcttcccaa
     3061 acttttttag catctaaacg gcgttttgaa agtaggtagc tgccgttttc tttatctttg
     3121 ccgatcgtac tgataacgac taaatctaat acatcaccga cttttactac ttcgctgata
     3181 tcttctaccg gcaaagttga taattctttt gccggaacga ctccttcaac tccagcccct
     3241 tcgattccga caatcacttg tttgtcttca atgactaaaa cttcgccctt aacaacatcg
     3301 ccaactttta cttcttgtac gcttttcatt gcgtcttcca ttgactggtt gttctcttga
     3361 ttttgatcaa attctgtcat aacacttgtc ctcctaacca acattctgcc gtaagtacgg
     3421 tactgttttt agtttaattg attattcaaa aaaaataaag cgatttcgtt cgttttcctt
     3481 gaaaaaacaa ttgtttttca ttttttttct taatttaaaa aagaagataa ccttttttca
     3541 aaacaacagc ttttattgct gcaacgactt ctggaatcga tttgccagtc gtatcgatac
     3601 gaacagcatc ctctgcttgc ttcagcggag agacgtccct tgttgaatca taatggtctc
     3661 tttcctgtat ctctttcgtc agtgtttcaa gatcagtcgg aattcctttt tcttggttct
     3721 ctttgaaacg tctttctgca cgctcactta cgcttgctac taaaaagatt ttcacttctg
     3781 cattcggtaa tacagctgtc ccgatgtctc ttccgtccat gacaacgcca ccgcgttccc
     3841 ctatttttcg ttggacagca actaattctt ctctgacttt tccatgtgca gagacttcag
     3901 aaacagcttt tgtgacatct ggttgacgga tttctaaagt cacatctttt gatgctatga
     3961 atacatgctg tccatcttct tgttgcgaaa aagagatagg atactttttg atcagcgaaa
     4021 ctagcccttc ttcatctgaa aaagatacat tgttcgtaat agctaaataa gttactgcgc
     4081 gatacatggc accggtatct gtataaatat aattcaattc ttttgctaat atcttggcaa
     4141 cggtactttt cccagatgaa gcaggcccgt cgattgcaat actgatttgt gtcatgatat
     4201 aactcctttt tctcagaaaa aaagccgcaa agcgcggcta ttattattta attcttaatt
     4261 cttgaccagg gtaaagcata aagttgtttg gatctaagcc attcaattca aacaattgat
     4321 cagtggtgat ccctgcacgc tcagccactt gttttggacc ttcgcctgct tgaacagtcg
     4381 ttgttgaacc ggctgcagct gatgaagaag gtgttgtttc tgctgcattg ctgcttgaat
     4441 cccctgcttg ataagttgcc tcacttgagg aagtcggtgt tgtctctgct gcaatactgc
     4501 tttctgacgt tacacctgta tcttgagaca cagtagattc ttccgcgatt gtactagatt
     4561 ctactgttga agtctcagtt gactcctctt ttgttgtact tgtagaactt tctactactg
     4621 atgtcgaagt ttcagaagaa gttgctgctg ttgcattgtt gcttgatcca tttctgatcc
     4681 agaaataagc acctgctgga atagcgatac ataagaccag taaaatcaaa aatagcgaaa
     4741 gaaataatgt atttcctttc ttttgttgtc tctgagaact tcttgatgaa acttcatcat
     4801 ctgtatcgta gattggttgt tcccatggtt cttgtgtttc gttattttct gtagattgat
     4861 gtctatcttt tctgctcact gttttacctc ctaaactcta tcgtgtgtat tataccatag
     4921 gaaaaagtgt cttgtcctaa aaaatcaatg ttccatgttt tttttaaata aatttagtaa
     4981 aatcgtttcc caatgaagtt gttcagtctt agaaaaaagc ttcctctttg gcagaagttc
     5041 tgcttttgct ccatcaatat cacaacaatt ctgcggcttt tcggagagtt tttcgccaaa
     5101 ataagctaag atgaattttc tgcggcagtt ttcttcatta atatagcgaa gcatctgctg
     5161 caggcggaat tctttttctt tttcctgacg tttgagccgt tccacccaat tttctggttt
     5221 ttcagactgc tggatcagct ccatccattt tttttgcagc tcatcaaagg gagcttgctt
     5281 tgtggcatat tccaaataaa gctcaaaact ttgtcgctgt tctctagaaa gttgattaaa
     5341 gaaataatgg atacgttcat ccccactttg ataaaaaaga attgctgcac tttcttcctg
     5401 atctctgcct gctcgaccga tttcttgtac atagttttct agactgtctg gcaaatcata
     5461 atggaccaca taacggatat cagatttatc gatccccata ccaaatgcat tcgtagcaat
     5521 gagaaactgc agctgatttt ttacaaactg ctgttgaagt tgtcttcttt gagctgcatc
     5581 aagaccaccg tgatagtatc caactgtaaa acgttccctg aataaatgat acaatcgttc
     5641 gacttctttt tttgttgcac aataaatgat tgccgctcct tgtgctcttt ccatgaagtg
     5701 ttcaagttct tgttcttttt gctgcgtttt ccgaacaaaa agcgagatat tttgccgatt
     5761 gactgattgt ctgatctctt ttggtggtcg atcaaatagt acatgacgta tatctcttgc
     5821 tactaaatct gttgctgtcg ccgttagtgc cagtgtgacc gggtttccta aaagctttcg
     5881 gatttttcct aactgttgat attctggacg gaaatcaact ccccattgag acacgcaatg
     5941 agcctcatct actacatata acgcaatatt ttgttttttc agatgctcta aaagagaagg
     6001 attagtcaac atttcgggac tcaaaaacaa aaatttatat tgcgatagat gacggaggac
     6061 gtatcttctt tcagattgcg aaagtaaact attgaaagca gctactcgtt tctcccctcg
     6121 tttttgtaat tgcgtcactt gatcttccat tagagaaagc agcggtgaca cgatgatgac
     6181 cagaccttcc attaaatacc ccgtcaattg atagcaaagg ctttttcccg ttcctgtagg
     6241 caaaatagcg agagtatctt ctccatcaag caacgcctga ataatttctt cttggccggg
     6301 acgaaatgaa tcaaatccaa aatatttttt taattcattt attaacattc tgtatcgctt
     6361 cctttctcaa tagatagatt tgatacaaac gaaattctcc ataatctaat tgtccatcta
     6421 cattccattc gctaaagcgc cactctcgca cggaatcctt atttggttcc aaacgttcga
     6481 ttgtttctgc agagagaatc cactcgtatg gaaaatcatc aaaaaacaag gcccactcaa
     6541 taagatgatc tgttactgtt cctcttttta aatgacggat tgccatgatc tggtcaattg
     6601 tctgaccatt cataaacatc tgcttggtga tcatcatgct ttgattgaaa ttttgctgta
     6661 ataatgaatc gatcaaagcg tgccaaagcg aatctggccg tttttcgatt tgtgctaaca
     6721 aaaggtggat acatctgctt tgttgcagat aaacctctga ctcatcattg ttttttgcca
     6781 gctgataagg aagcagccct gttcgctcat ttccagataa ttggttagct aaaaaatctg
     6841 ctgcttcttc tggtatctcc gaaaacagct gcaccaaact ctcatacgca gtatcaatca
     6901 aaatttcacg tggcaattgg ctttcactaa gccatttttt tagttggaaa gtataaaaag
     6961 gacttgtctc tgctggaaga tagtcttgaa tacccgttgc cagattagaa attacttgaa
     7021 cagcaaattt aatgagccgc caactagttt cccctgttct gccatagcgg tcaaaacgca
     7081 aaccagtatg ttctatttga tgttcggaca acatatcagc ccctgcaggc gttagctttg
     7141 cttcattttc atttatttcg atccaacctt gctgcgcaat tttttgcaaa atttgataaa
     7201 acttatcctg cttcaattct ggaaaactgt tatggataaa caaaagttcg tgggtaaatc
     7261 cataaataag tactgaagtc gttcgttttc ccacaaggag ttggtaaaga gaactaatcc
     7321 gcagcttgtg tcctgatgaa aatagcgata aaataaaaac aatcatatta ttctccttaa
     7381 gttaggatgg ttagaaatgt cactactatg taaacttgta cctgaacgct gtattgcatg
     7441 cggtctttgt caaatcgaag cgccagatat tttcgattat gatgaagaag gaatcgtttt
     7501 atttgcccaa gaaccagagg ctcatcaaca gttcgtgatg ccggaagaag aaacatatgt
     7561 aaaaaaagcg tatcaaaaat gtccagttcg agcgatttta ttagaaaata aaatataaat
     7621 ataaacgaaa ctttaaaaac gtacactaag ttctctaaac ttgaaaataa gccgggatct
     7681 gtttctcttg ttccgtgaaa tcccgactta ttttgaaaga ttcatttgct atctatagtt
     7741 aattgactag ttttttcacg gaccctatag gacccattta tattatttca actttttcag
     7801 ccagttttcc aatgtattga cgatcacagt ttgctggtct gcgttagaaa tttctgcctc
     7861 tccatctcct ttttgctcac catagctgcc aaaacctcca tgatttccgc cattgattga
     7921 tacatactct gttctttccg gtaaatactg cttagcttct tgataagctt cctgattcaa
     7981 tactttatct tttgtcgccg tgatcgataa aacaggaaca gctgattctg tcaatgttcc
     8041 ttttttgtcg ggataacttg ccatgaagaa tacccctttt aaatgtttgt ctgaatgatt
     8101 cttattgaca taacgacttg ccattacgcc acctaatgaa tggccaccga tcacataatc
     8161 ggaatttgga aaatcttctt tgaccttatc cgctttgttt ccgcccatca ctgctagatc
     8221 aagtggcatt ttgacaatag cgactgaaaa gccgtttttt gctaaatctt gcgcccaaat
     8281 actataactt tctggctcta cgagagcacc aggataaaaa ataagtaatg gagactttcc
     8341 atgattcttt ttctctcctt caaaaaataa gtaatcttct ttgttttttc cttcttttac
     8401 caacacttgc gcttctgacg atggttcgta cgtattttgc tgtatatagt aataaccggc
     8461 tccaccgagt aatcctaaaa tgatgactaa gccaattagt attcgtctaa atattttcac
     8521 tttctcccac cttttctatc tgagtgaccc ctttttctta atcaagtatg attatacctt
     8581 aaaaaaataa gagagaaatg caaactttcc agcatcaaat aaaaaaagac ggaatatcca
     8641 tttgaatatc cgcccttttt gtatgtttat ttttatttag cacataaacg atacacagct
     8701 tctgcataaa tttccattgc tttatacata ctacttaatt cccatcgttc attcgcctgg
     8761 tgcataaagt caggagtatc agggaacatc gcaccgaatg ccacacactg gttcatcgta
     8821 cgagcaaaag tagcgccgcc tgatacaaac ggttcagtca tgtctccagt aatatcgcga
     8881 tatgtcccca atagattttt cactaactca ctgtctagtg gcacgtataa tgaatcaagg
     8941 aagtcaaatt cttcataagt caacccatat ttttctgctg ttttagttaa tttagctact
     9001 aaatcatctt tcttgaatgt aacagggatt ctcatatcta caccgatttt tgtttgttct
     9061 ggtgtgattt ccaaacttgc aaagttcatc gttagctcgc cggattgttc atcttctgtt
     9121 ttgcccacga cagcttcacc agttgcattt tcttgaacta attttcctaa gaaattgatt
     9181 gggccaaaat caaatacttc tgaaagagca attgccagac gagaaatagc attcactcct
     9241 tgtgctgcat ctttcgcgtg gatactttta ccaagaacga cgatgccttc accttgttct
     9301 tcgaaatcga attcgtgttt tttcaatgct tcttttactt cagaaagctt ttctccagag
     9361 taaggagctg catcaggaac aacattcaac gctcctccag cttttacaga aaactcgttc
     9421 gttcctggtc ctgtcaagta agcttgcaat aagccttttt ctgcataaat taaaggaaat
     9481 tcagcatctg gcgcaaatcc ttgcgtaatt ccttcttctt tttcgttata tttttctaaa
     9541 caacgccata aattttcttc atctgtacca aagataaaac gaatacgtgt cttgaattcc
     9601 acgccagcat caatcaaagc tttgacggca tacattgccg caatagaagg acctttgtca
     9661 tcttgggatc cgcgaccaac taaccagccg tcttttatag ttggatcgaa tggttttgtt
     9721 tcccaattat tttcgtcccc tgctggaaca acgtccatat gacacaagat accgaataat
     9781 tcatctccag agccaatctc tgaataaccg taatagcctt ctggatcttt aaatgttcta
     9841 aatcctagct tttcactgat ttccaatact ttatccaaag cttcaatcac ttttttaccg
     9901 aagggatgtc cttgtcctga atctgcttca tctagtacgg aaggaattcg gatcaattcg
     9961 ctcaatgatt ctaatgcttg ttcatgatgt tgttctgtca caaatttttt catgatatta
    10021 tcctcctaat atgttaaaaa gggtcatgtc aaattcctcc gacacaacct cttcttctca
    10081 aataatgacg ttttaagcct gctccatcag caagcagaat gttcttaaca aatgatgaaa
    10141 gaaaaaacac tcactgaatg gagctattga cttgtccacg tgcttatcga tagccgttta
    10201 tcggtttctt cattcttatg atagaccttg tacctgtatt tgtctaggtt tagaacattg
    10261 ctgctacacc taggatcaca cagatggcta caaataatat cgcaattaat ttaccggtaa
    10321 atttccacca cactgaaaca tcgaaacgag caattgctac agcacccatt acgatagcag
    10381 atgttggtgt aattaagttt actaaaccag atgcagattg ataagcagtg attactaaat
    10441 ccttaccgac gccggcaaat tcacccatag gtcccatgat tcccattgtt gcagcagcta
    10501 agccggaagt tgaaggaatc aagaatgaca tagggatata gaaaataaat gtaataatga
    10561 tgaatacact tgaagataat gaacttaatc cttgttcgcc ccaatgtaag atggtgtctg
    10621 tgattaatcc attgttcata accacttgga taccacgagc aacagctact acgattgcta
    10681 caccgatcaa atcagaagca ccagctaaaa attcagagac aaattcagac tctttcatgc
    10741 cataagtgat tgctactacg atggacatta ctaagaataa catagtaatt tctgggaagt
    10801 accagtcacc taatggaagc atgcttttac ctagaaccgt acctagtacc ggaaggtttg
    10861 ttaaccagtt tgtcaatgat tcaaaaatcg tgaaattcgc attcaaggtt gtccaaggaa
    10921 tcaaactgat gatcatgata ccaaaagtaa taaagaacaa tacattaaca tgtttttgtt
    10981 ttttcgtgat tacttcttga cgaccgattg cttcaatatc gaaatgcttc atatcttctt
    11041 tacgttggtt gtatactaat gattttgttg gatctttttc gatctttgat gcataacgat
    11101 acacatatac aatagaaata gctaacaaaa taacgaacat caagaatcga agaccaatac
    11161 cttcacccat cgaaatccca acagcttgtg aagctacgcc tgttgcaaaa ggatttaccg
    11221 ttgaagctag acagcctact tgagacccta ctaatacgat tgctacagct actactgtat
    11281 caaagcctac agccatcatg actgggatca gtaaagcata gaatggaatc gtttcttctg
    11341 ccattccgta agtagagccg cctaatgcaa ataacagcat caatactgga ataagcattt
    11401 tttcttttcc tttgaatttc ttcacaactg aagcaatccc ggcatctagt gcccctgttt
    11461 tgttgataat acctaaaaat ccaccaacaa ccaaaatgaa gaaagagatt gggattgcag
    11521 caggtgtttc tttagttagc tcccccgctg gtacacctag catcccattg attggcgcca
    11581 tgaaaatatc ccacagccct tgaggatttt gttgtactcg ttcatatgat ccagcgacga
    11641 tgttgcctgc atcgtctaca ctatagtgcc cagctggtat aaaccaagta agtacagcga
    11701 ttactgcaat aataataaat aagaccgtat acgctgaagg catttgaaat tttttctttt
    11761 ttgtttgttc catcttcttc ttcctttctt ttccgttaga gacaacaatt ttggctctaa
    11821 tgttatttta tcaaaaagag tatcagaaca aaaataataa cacgcatttt gtaaaacttt
    11881 tgttcaaaaa gttcgttttt ccctattccc tcctcttttt tcaggttttt aaagctttct
    11941 tctttttcat tgtagcgttt cctttttttt ataaacaaac attttcgtct tttaaaagga
    12001 gaagaatttc acatttgcaa agaattgatt gatttttatg cataaatcaa aaaaatactc
    12061 aaaacaaaca aataaaacgt ttacattatt gcatgtcttg tatttttttc caaaaaaaaa
    12121 gaccaagaaa tttcttgatc tttttgatat ttatttgact gctgtatgac gttgttcgaa
    12181 ctgatgttgt tttcgactta accatggaag aagttgcgca tgcagcacta aaaagacagc
    12241 actcacgatg aatcctttga tcaagttgaa aggaacgatc ccaatcagta catagtttgc
    12301 taaagacatg cccagcatct gtgttgcgtt cagccctaaa aacattaagt aaagaggcgt
    12361 aataacgaaa taatttgcga tactcataaa aatagttaga gcgatggttc ctgtaatgac
    12421 acctaaaaat ttgtttcttc ctttatgctg tttctttaca ctaaagaaat aataaatcgg
    12481 gaatgtaaag atcgttgttg ataagaaact agcagtgtct cctaccaaat tatccggaga
    12541 aaaacctgta gtcagcagat gtaatactga acgcaagaac gctgttgcaa ctccggccgc
    12601 tggaccgaat aagaacatgc tgatcaatac cgagatatca ctaaaatcga tcttcaaaaa
    12661 actaaaagcc ggcatgatcg gaaaagcaaa atactgtaaa actaatgcga tcgcagcaaa
    12721 catcgctact gcaaccattt tctgtacacg ggtgttcttc atttgaaatc ctcctgttag
    12781 gactcctctt cagcgaactt tttgacgttt ctctccaaat gaaaaaactc tattttccag
    12841 ggaaaataga gttagagtta tttgatcaag cgtcgtcaaa taagctctat cttctttatc
    12901 cagactatac tgtcggtatc ggaatttcac cgattcagct atttttttgc aaaataggtc
    12961 gtggactata accaccggtc gggaatttca ccctgcccct gaagacgaac cttatataat
    13021 ttttgtttac atattcatta tacatatcta ttcaaaaaac gcaacatcta acacttataa
    13081 aaagtaagtg ttcattttga gtggttcagc aaactgctga cttctttttt gttcagttca
    13141 cggtaatccc caggacgtag gccttgtaaa gtcagatctc cgtatttttc acgttttagt
    13201 ttttgcacag gaagaccaac tgcttgaagc atatttttca cttgatgatt acgtccttcg
    13261 tggatcgtca attccacgat actgtggttt gtcttaggat ccactgataa aatttggaat
    13321 ttagctggag atgtttttcg tccttcgatc tttattcctt ttgtcaaagg tgcaagcatc
    13381 gattttgtag cgatcccttt tacctttgca acgtaaactt tatccacttc atgttttgga
    13441 tgtgtcaggc gctgagcaaa atctccatca tttgtcagta gcaagatccc cgaagtatcg
    13501 taatccagtc gacctacagg atagatacgt tcttttacca tcggcaaaag atccgtaaca
    13561 acttttcttc ctttgtcatc tgcaacagca gaaataactc ctttaggttt gtacaataaa
    13621 tagtaaccgt attcttcttg ataaattggg acttcatcca cttccacagt gtcatgtttc
    13681 ccaactttta tccctaattc ccttacaact tgtccgttta cttttacacg tcctgcaagg
    13741 atgaattctt ctgcttttcg acgagaagtg atgccagcgt gggcaattac tttctgcaat
    13801 ctctccatta caattattct cctttattca ttccatcaaa gaatagatct ggtgacacgt
    13861 cttcttcaag tgaaatttcc atttcttgtg tatcaggcag ttcttctaat gatttcaaac
    13921 caaaataatc taagaagtag ccagttgttc catataatat cggccgacct ggtccttcta
    13981 ctctaccttt ttcttcaatc agtttatggg cagctagacg ctgtaccgct cctgaaacct
    14041 gaactcctcg aatctgctcg atttcagctc ttgtgattgg ctgtttataa gcgatgattg
    14101 ctaaggtttc gacggcggct tgagataatg tgtttgatgt cccttgcgca tatcgcttaa
    14161 gtaagggagc cagctctttt ttagtcgtta atacataatg gctttccgtt tcaaaaatat
    14221 gcaaagctga gtgcgagttt tgagtgtaag attcttttaa ttctttcact aaacttgtga
    14281 cttctgcttc gctgagattt aacagtccgc ttaattcttc attactgatc ccttcctcgc
    14341 caacaataaa aagaagggct tcaagttcac ttaatttcgt catgttcggt atctcctact
    14401 gctggataaa gcataattga ttcatagttt tctttttgtt gcgcaatgat ctgccctttt
    14461 ttcatcaatt ctagcaaagc cataaaagta gtgacaactt cacttttaga aggtgactca
    14521 aaaaaagaat cgaaagtaca gccatgcgca tttcttttta tacgctcttc gataaatacc
    14581 attcgttctt cgatcgttgt gtcatcgggc gtgatcgttt tttccacttt ttgagaaaat
    14641 ttcttacgtt ctaagatttt ctgcatggca cggatcaaat cgattttttt gacttgtcct
    14701 cgcttcaacg gcttttcagc atccgctaac ccatcgatgt tcataggttc ttttgtgtaa
    14761 taaagacttc gttcctctgc tttttctgtc agtctttcag ctgcatattt atatttacgg
    14821 tattctaaaa gctgagtgac caattcttct cgaggatctt cttcccataa ttcttcatcg
    14881 tctgtgccga attcttggac aggaagcaat gtcttgcttt tgattgccat caatgtagca
    14941 gccatcacta agtattctcc tgcaacagct agctcgagtg ttttcattgt atggatataa
    15001 ttcatatatt gctccgtgat cgtagcgatc ggtatatcat atatatcgat ttccatttgc
    15061 tgaatcagat gaagcagcag atcgagagga ccctcgaata catctaattt aatattgatc
    15121 tccgtcaact cattcactct ttcttctttt tctttgctag ccattttcca atgtaaggct
    15181 ctgtgatttc agttgcacgg atggagacat ccgggtactt ttcctgtaat tcatctaaca
    15241 tttgaaagcc catattctca ccgcgataag acggattcaa agaaatgtca tggatgatca
    15301 gctgatcgtt cgttgcccag taaataccaa taatcccttg aatgttggtt gaaccttctg
    15361 gcgtaaagaa aaaaagttca taatcgtctt cagattcgta cgtatcaatc tccttcaaca
    15421 aagaagtttt acctgtcagt ttcttatgga aactcaatag tcccatagca attttacggt
    15481 tttctttccg gtaatgtgtt aacatcttct caccttccta tgactaagcc cttggaaaat
    15541 gctgcttata aacatccgtc attcttttct tcgtgatatg tgtataaatc tgagtcgtag
    15601 agatatccgc gtgacctaac aattcctgta ctgtccgcaa atcagctcca ttttctagca
    15661 gatgtgtggc aaagctatgc cgcagcgtat gtggtgttac ttctttgtaa atcccagcct
    15721 ctcgtactaa ctgcttcaga tttttccaga ttccttgccg agaaagacct ttcccatgat
    15781 tattgacaaa tacatgcatt tcagatgcgt cctttacaag taatggacgt gcttcgtcta
    15841 agtagcgttc cagccattgg atcgcataat cacctaaagg aatgatccgc tctttatctc
    15901 cttttccaac tgtctgtacc agtccaagag aaagatgaag atctcccaat ttcaatccaa
    15961 tcaattcact gacgcgcatc ccagtagcat acatgacttc caagattgca cgatctctta
    16021 tccctaacgt ttttgtcgta tcaggtgctt caatcagctt ttctacttct gtcagagtca
    16081 atgtacttgg taatttctgt gctttcttcg gcgtatcgat atgttgcatc ggatcatgat
    16141 cagtgatacg ctcttgccgt aaaaactgat ggaatcttct gagacttgag atcatccgag
    16201 taacagttgc tgtcgctttt ttttcttcat gcaacttctc cagaaaacgg atgactaaaa
    16261 agcgatcgac atcctgccat gaagcaattt tctgctcctg tagaaaatgc aagtactgtt
    16321 ccaaatcacg ttcatagctt tgtcgtgtat tggcggacaa tccccgttca atattcaagt
    16381 agtggagata atcgttgatt tgctctttca taataatccc cctcatgcct ctctatcata
    16441 tcagataccg cttcattctg atagtctttt tccataatct gagcaaagaa aaagccgtct
    16501 agacactcag acggactggt ttcttttt
//
LOCUS       DACEQH010000059        16348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.060, whole genome shotgun sequence.
ACCESSION   DACEQH010000059 DACEQH010000000
VERSION     DACEQH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.060"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IW833_002126"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IW833_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ1308225.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="IW833_002127"
     CDS             complement(2944..3378)
                     /locus_tag="IW833_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308226.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..4600)
                     /locus_tag="IW833_002128"
     CDS             complement(3395..4600)
                     /locus_tag="IW833_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ1308227.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5294..5680
                     /locus_tag="IW833_002129"
     CDS             5294..5680
                     /locus_tag="IW833_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1308228.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5774..5965
                     /locus_tag="IW833_002130"
     CDS             5774..5965
                     /locus_tag="IW833_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1308229.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6479..6811
                     /locus_tag="IW833_002131"
     CDS             6479..6811
                     /locus_tag="IW833_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1308230.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7107..7262
                     /locus_tag="IW833_002132"
     CDS             7107..7262
                     /locus_tag="IW833_002132"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1308231.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7538..8878
                     /locus_tag="IW833_002133"
                     /pseudo
     CDS             7538..8878
                     /locus_tag="IW833_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9119..10735)
                     /locus_tag="IW833_002134"
     CDS             complement(9119..10735)
                     /locus_tag="IW833_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1308232.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            10853..11071
                     /locus_tag="IW833_002135"
     CDS             10853..11071
                     /locus_tag="IW833_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1308233.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            11266..11727
                     /locus_tag="IW833_002136"
     CDS             11266..11727
                     /locus_tag="IW833_002136"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1308234.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11920..12159)
                     /locus_tag="IW833_002137"
     CDS             complement(11920..12159)
                     /locus_tag="IW833_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308235.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12183..13706)
                     /locus_tag="IW833_002138"
     CDS             complement(12183..13706)
                     /locus_tag="IW833_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1308236.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(13724..13846)
                     /locus_tag="IW833_002139"
     CDS             complement(13724..13846)
                     /locus_tag="IW833_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1308237.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(13876..14859)
                     /locus_tag="IW833_002140"
     CDS             complement(13876..14859)
                     /locus_tag="IW833_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1308238.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14883..15032)
                     /gene="rpmG"
                     /locus_tag="IW833_002141"
     CDS             complement(14883..15032)
                     /gene="rpmG"
                     /locus_tag="IW833_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1308239.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(15053..15430)
                     /locus_tag="IW833_002142"
     CDS             complement(15053..15430)
                     /locus_tag="IW833_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308240.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15462..15641)
                     /gene="rpmF"
                     /locus_tag="IW833_002143"
     CDS             complement(15462..15641)
                     /gene="rpmF"
                     /locus_tag="IW833_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1308241.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15656..15925)
                     /gene="rpsN"
                     /locus_tag="IW833_002144"
     CDS             complement(15656..15925)
                     /gene="rpsN"
                     /locus_tag="IW833_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1308242.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
BASE COUNT         5184 a         3096 c         2455 g         5613 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtcctt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4381 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4441 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4501 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4561 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4621 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4681 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4741 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4801 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
     4861 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
     4921 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
     4981 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
     5041 taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat
     5101 gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga
     5161 aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag
     5221 cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga
     5281 ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt
     5341 tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa
     5401 acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa
     5461 acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat
     5521 ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa
     5581 aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa
     5641 acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt
     5701 ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga
     5761 agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga
     5821 agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag
     5881 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     5941 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6001 tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataattat aaagtatcga
     6061 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttatcaacgt
     6121 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
     6181 ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg tacttcaacg
     6241 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
     6301 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
     6361 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
     6421 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
     6481 gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
     6541 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
     6601 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
     6661 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
     6721 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
     6781 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
     6841 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
     6901 gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt tgggtgatgg
     6961 atattactta cattccatgt aatgacggtc gcctctattt gtccacctat attgacttag
     7021 caactcgtat tctacgttac tatatggtag attcacacat gaaaaaagag attgtcgtcc
     7081 gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat agaggtagcc
     7141 agcatcggtc ttttatttat caagagctgt tgaacgacca ccacatcaca cattcagtga
     7201 gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga tcaatcaaat
     7261 gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg ttaatccaag
     7321 attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg atgactcctt
     7381 gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg acgccttctg
     7441 ttgccaatac tttttctttt ttctaataaa cgcaaacaga caccttaaaa tcctttagaa
     7501 taaagtcgac gaaaaccatt caaaggagtg tttcaagatg tcctattcag agtttaccaa
     7561 agagttacta gatattctag acctaaatct tatctttcat gaagatgcct ttcgcaaaga
     7621 gcgaatcaat gacgaaacat gttttgtttt tgatggcaca ttgacctatc aaccggagga
     7681 atgctttcat cgccactatc aaaatgaaca aacgatcatt aaatagggct ggaaaaaagt
     7741 atccatctta ttgaatgatg tcagtaacta caagaccatt ctgcggatta acaaacagcg
     7801 cttcaaatgt aaacactgtg gaaagacttt tttagcagaa gattcagtta gtgatcgtcg
     7861 ctgctccatt gctcgcagag tcaaacaagc cattcttgag ctattaagcg aacctctttc
     7921 tatgtctttg atcgcacgta tgaagcacat ttctccgacc actgtcattc gaatacttcg
     7981 cagccttcgg cctaaaacca tttctttgag ccagctgtta ccagaagtgg tgtgtttcga
     8041 tgaattcaaa tcagtgaaaa atgtctcggg agccatgagc tttgtcatga tggacggcaa
     8101 gacccatcaa ctgattgata ttgtggaaaa tcggcaactc aatcctttac gcgactattt
     8161 cttgcgctac cctataaaag tccgtgaaca agtgcgtcta gtggtttcag atttctatac
     8221 accctatcgc acattggtga aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt
     8281 tcatattagc caacacattg gtcgggcttt taccaaccac cgaattcaag taatgaagac
     8341 ctttaaaaaa ggggatcgtc ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa
     8401 gaatgcgtgg gaattgaaag gtcagcatcg ctactggcgt ccgtcatttc gagatcatct
     8461 gacagaagca gagatagtcg atcggctatt atcttatgat gattccttaa agcgaggcta
     8521 cgaagtctac caggattttc tttccgttat cagaagacaa gatgttcctg aattcgtcgc
     8581 gctcctaaaa gaagactaca aggagttgcc agagcactac caaccagtgt tcacgacctt
     8641 caagaaatac caaacagaaa tcaaacgagc gttgcgcgtt ccctattcca acggccctat
     8701 agaatgtttg aataaccata tcaaagtact aaaacgaatt gcctatggct atagaaactt
     8761 tcaaaactac cgagaacgaa tctttttata tcgaggaaaa tattttaaga aaactaagaa
     8821 tactacccaa ctgactaaag ccagaacaac aacacggcta gacaagatag ctgtctagcc
     8881 gtgtgagtat ctcgattatt ctattgaatt tcttcaccaa cactcattga cgaagagcca
     8941 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
     9001 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
     9061 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
     9121 agtaaatgta gtaatctgtg ctttcttttt tctcaatact aataactaac ttatggggta
     9181 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
     9241 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
     9301 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
     9361 tttccttgcc atcaattcga acgatggcat atttgatagc atcttcagtt gtttcattct
     9421 ccttttctat agagaataca aataagggaa taaaagaggt aataatcaaa atgccaataa
     9481 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
     9541 taaacatatt agactttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
     9601 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
     9661 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
     9721 cgggattttt ttgtataatc atttgacact caattttagg taaactagct acccaataat
     9781 ttaatgattc aatactttta tagctacccg atgtttcaaa gatcgtcgaa aaatcactat
     9841 gttgggcaaa ccaaagaaac ccttttgctc ttgtaatatt gccaggaaaa tttttatccg
     9901 taagatgggc aaatttttgt gcgtcaagga gtaaccgatt tcgatagata aaacttgaga
     9961 taccatattc ttctgtttca ggaatatgat tttgatagcc aagctctaac tctttgaacc
    10021 agcccactga ataggaagtc tcatctatat taaaaagatt tgtatttaga attttcgata
    10081 agtctacttc acctttaatt gtcctaatta ttttggcatt ggactgtaac ttagcaatta
    10141 gaccgttaat tttgtaaatc gtcttggttg acactaagtc actattattt aaaagtaata
    10201 tgttacaaca ctcgaattga tcaattaaca agccacgaat atcctgttct tcttcaacta
    10261 cattaccttc agcttttctt tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac
    10321 gttttacatc cactactata ataatggcac caatctctaa tcgttgggtc aaatcaatgt
    10381 ctaaagggtt tttagctaat ataattgctt gaataattgg aattggtttg ctgattcctg
    10441 aagcttcaat aacaatttga ttcaagtcag aaaattgcac taattgatct agttcaacaa
    10501 tcaaatcttc gcgtaatgta caacaaatgc atccattcgg catcgaaaca agtttttctc
    10561 cagttctttt aaaacctttc ttgtcgacta ataccccatc aacatttact tcccctatgt
    10621 catttacaat gacagcaatt tcctttccgt gatcaccgta cagaatatga tttagaagta
    10681 tcgtttttcc tgaaccaaga tagccagtta agattgtaat aggtattttt ttcataggga
    10741 acctctttct ataccttgaa tttacacttt aagtgcaaca cataaaaatg agtcataatc
    10801 gatatccaac tagtataagt ttgtcgagaa cactcctaga aggaaaataa ttatgagcca
    10861 gtatcgtcat cttactatac tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt
    10921 ttctattagt ataattggca acgacattca gcatagcccg tttactattt tacatgaact
    10981 gagaagaaac aagaaaaaaa ctattcacct tccaaagcgg aaaaaaatta tgcttatcga
    11041 agaaaaaaat gcggacgtta tcacattcta aatgatccgt gagtttggtc aattgttcgt
    11101 catttatttg tcgattttca gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa
    11161 catatggact tttcaattag ttttatcacg atctaacgtg caatttgcaa tgatcttttc
    11221 gatacagaac ccctctcaca cggcaataaa ggcctgatcc gttcattgca ccatcgagga
    11281 aaaacaagac atacaaaaaa tcatgtcgag tctcgtggaa agatttgcct tactcattcg
    11341 atccatggcc gtccaaaatc cgcagatgat agaagtgatt ttggtcattg gaaagccgat
    11401 attgtagctg gcaaaactgg tggtacttgt ttagtgactt tgacagacag aaaatcatgc
    11461 ttccttttag caggaaagaa ggtccccaga aagaaagccg cttatgtgag cgagaaaatg
    11521 attgaacttt taagcagtat tccaaatgag gcggtcaaaa cgatcactcc agatagagga
    11581 aaagaattct ccaatcattt cgcagttaca gaagcattga ataatatgcc cttttatttt
    11641 ccagatcctc atgcccttgg caacaatgaa caaattaaaa tacgaataga ctcattcgag
    11701 aatacttgcc aaaatcgaaa gagatagcta cggtagataa ctcaacgata aatcagatga
    11761 ttgaaaaact gaataacaga cctagaaagt gtttagattg gagaacacct tatgaagtct
    11821 tctttgataa agtgttgcgc ttgatttgac aattcaagac ataataaaaa gcagatcaaa
    11881 tccaaataag ggaaaaatga tctactctgg tattatcaat taattggcca agctattatg
    11941 ccgttttgct aaacgtctac ttcttccagt attgaccttg ataaaacaac aatcaggatc
    12001 ccgttttaat actctatgaa ctgtgtcaat actaacatgt aaagttgcag caatatcagc
    12061 taatgtactc ccatttaatc tcatttgctt tgcaaatcga taacgccgat aagcatcaat
    12121 attcttggaa tccatagaca ataatacttt tcttaccatg tatttcctcc aaaaattgaa
    12181 ttttaatgag ccatcatttc ttgagcaatt tcatgtttag acaatgaagc tggatagaaa
    12241 gtaggccaat tatgcaattc attatagagt ttttcttgac tttctccacc aaagaaaata
    12301 tggaaatgct ctgccttact tggtgcaatt ccatgatcac taaattgaat atatttaaaa
    12361 cgaccagcac ctgcatcatc agtttcaaaa ttaaaacgaa caccacggtt tcctttttca
    12421 taattcaaaa ttttgtatcc aacatatttg tatgtgtatt gatgatgttc accgttgact
    12481 acaaaatcaa tggtgttgtc tttaatgttg atagtatcaa tatctgtctt atagccagta
    12541 gtataataat ctttgtactc ggcagctgtc atgtctttat tgatttttgc tttgtaatcg
    12601 aaaacttcat ccaaaattcc atcttttaac aacggataaa cagattgcca ttcgcctgta
    12661 taatcactca attcgcgatc cttcacatcg gcatctttaa agtacccatt agcaactgtc
    12721 ttttcatcct tcgcttcttt ttctggaact acctcttcac cagcaaccat agttgttttt
    12781 tctaacgctt ttagattgtc ttccattact gaaacataat cttcaccatt atccatttgt
    12841 tcttttgtaa gactttcaag aggattcagt acttccaatt ttatcccagc ttcgttggcc
    12901 aaagtttttg caattttatc attagcattc ttttcaaagt aaatataatt gatcttattt
    12961 tttttaacat actcttttaa ttcagctaat cgaccagaag atggctcttc ttcaggactt
    13021 agtccagcaa taggaacttg tttcaatcca taatccaaag ctaaatatcc aaaggctgcg
    13081 tgttgcgtca caaaactttt ttgtttcgct tctttaagac tagtggtata gtcagcatcc
    13141 aaccgtttca gtttagtcaa atacttttca gcatttgtct caaatacttt tgcttttttc
    13201 ggatacaatt taactaactg atccttgata tttgaaactt ccttaattgc cattttgggt
    13261 gaaacccacg tatgaggatc tagttcatga tggtgatctt cttctccgtg atcatgatct
    13321 tcttcttcac tacctggcaa taaaatcatg tcttttgttc cttctactac attaggccca
    13381 gctttcttcc aactttcttt agcttttggt acccatgatt ccatgttttc gttatgatat
    13441 acaaaaacat cagcatcatg agcttttgcc atatccttag ctgagggctc atagtcatga
    13501 ggttctgatc ccgctggaac cattaaatcc acgttcgctt catcgccgac gatgtttttg
    13561 gtgaaatcat acattggata gaatgttgtt acaatttcta attttccatc tttatcattc
    13621 gatacttttg catcattagt gctggagcaa gctcccaata atcctagagc tgctatccca
    13681 ataagtccta cactgatttt cttcattctt tttccttcct taattatgct aatttcttca
    13741 ttttatttcg tttatgttct ttgataattt ttaatgccct ttttctagtt ttaatattcg
    13801 ggcttttaag tcgtcgatat gcactagata tatccgtttt ctccattaga aattaccccc
    13861 aatcactact tatcattatt tgctttccgc cagtaattta tcaaaactct gtttgatcct
    13921 ttcttcatcc aacatttttc cgattagaat aatagtggtt tggtcttttt gtcgagtggt
    13981 gtattgaaaa ttaacctgtt cgtttacctc ttgcatggca actataaaat cctgctcttg
    14041 taaagcaatc aatcctttaa aacgatacag gctcccttgg ttggttaaaa tcagccagtc
    14101 aatccattgg agtaaaaaat ctttttttat gtcttgatct gaagttaaga gaattgattt
    14161 aaactcatgt acttgatggt gttgatgata gtatttgcca ttatagtgat tttctgatga
    14221 atcactttca aaaactaagg gatgattaaa ttttttcaaa ttataaaaat cctttttttg
    14281 aatcgtttca ctatgtgtga acattcgaat atcggataaa gggttaatac ttttgatttc
    14341 tctctgtaat ttttttagtg cagataaatt caagttttgt tttctagaca caaaaatgcg
    14401 atctgccatg acaagttgct taagggcttg tggttctctt tcaataagtt caaaactgtc
    14461 actatcaata acagtcaata aactatcgat atatactctt cctcttagtt gaggcgctgt
    14521 taaaagtgtt tgaatgattg gttggggatc tgctatacct gtagtttcaa tgatgatttg
    14581 tgaaattggt tgttgatgtt cttctattgt ttccagtaaa gaaatcaata ttgctaataa
    14641 atcactgcga agactgcaac acatacatcc attatttagt tgtaacaccc gttcctcgct
    14701 atgaactaat aatttgtgat caatcccaac ttcaccaaac tcattttcaa ttataataat
    14761 ttcttccctg gggtagggtg agacttgcaa ggcttgattg attagcgttg tttttccagc
    14821 ccctaaaaac ccagaaacga ttgtaactgg tatgcccatt ttgtcacctt tttaccactt
    14881 agctatttca cttctttaaa aattgcccgt ctgcgcaatt ttggcgaata cttttttaat
    14941 tctaaccgtt cagggttatt ccgtttattt ttgctggtga gatacagacg ttctccagtt
    15001 tcgacacatt ccaaaataat attttgacgc ataaatcttc ctccttcaat acttatcgat
    15061 cttcaagata tgcttgaatt tgttgtgcat cttcaaaagt taactcgcca gattccaatt
    15121 gtgaaactaa attttctttc atatcaacag gtatctccca ataaccatcg atacattgaa
    15181 ataactcctc gtcatactct tcaaaaattg gatagattct taaaaaatca tcaaatgata
    15241 agccttggtc ctcatagtaa tctttgacta atccattttc aattgcctgt tgggtcggta
    15301 aacccgtttc agtaataatt cctctctcta ccatcgaacg ataaaaagtg catttatcta
    15361 gcgattgctg ggtcgtttca agataatact ttaatccttc ttcaactaaa ggtgggtaaa
    15421 taatttccat tttgattccc tcctaaaagt tatatattaa actattttga agctccttta
    15481 attactttct ttccattata gtaccctttt aaagatacat gatggctacg tctataatca
    15541 ccattcgctt cgtcaaatga aatttctggt tttactacag tttgatgagt tcttcttaac
    15601 cgtttcttcg ccttagatgt ttttcttgct ggtactgcca tgatatttcc tccttttacc
    15661 aacttgcttt ttttactcca ggaatcaacc cttgatgggc aagttctcta aacttaatac
    15721 gggacatacc gaactttcgc atataccctc ttggacgacc atccgtctga tcacgaagcc
    15781 tcagacgatt cggatttgaa tctcttggca atttagcgag agcggtgcga tctcctgcgg
    15841 cttttaattc ctgacgttta tctgcatact tttcaatcat cgccatttgt ttctttgctt
    15901 tagcaatttt cgactttttt gccatatacc tttatactcc ttttattttg atttagacac
    15961 ctcaaatcgt aatcgttacg ttttgacact gatactttac caacaagagt tgctcttgtc
    16021 aacaataaat aacatgaaat aaaactcata tgaatagata actatttgaa aacgaattta
    16081 tcggtacata gagataatca aatcatttaa aagaatatgc agtttaggct ctgaaaaata
    16141 agaattcact tttattagtc caatcaaaca caaataccgt tttaagtagc ttttcctcta
    16201 cttaaaacgg caatatgaaa gaaactataa ctttttattc aatcttttat gtttaactgg
    16261 tttatcgcca attttttaaa actttgatta ttccttatat tataagaata gacattaagt
    16321 gaactaattc aatcatactg attctttc
//
LOCUS       DACEQH010000060        16147 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.061, whole genome shotgun sequence.
ACCESSION   DACEQH010000060 DACEQH010000000
VERSION     DACEQH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.061"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..783
                     /locus_tag="IW833_002145"
     CDS             <1..783
                     /locus_tag="IW833_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1308243.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IVVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFAEKW
                     KTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQNEE
                     SMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQPMEN"
     gene            1161..3203
                     /locus_tag="IW833_002146"
     CDS             1161..3203
                     /locus_tag="IW833_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1308244.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            3214..3834
                     /locus_tag="IW833_002147"
     CDS             3214..3834
                     /locus_tag="IW833_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ1308245.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            3846..4304
                     /locus_tag="IW833_002148"
     CDS             3846..4304
                     /locus_tag="IW833_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1308246.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            4323..4601
                     /locus_tag="IW833_002149"
     CDS             4323..4601
                     /locus_tag="IW833_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1308247.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            4626..5993
                     /locus_tag="IW833_002150"
     CDS             4626..5993
                     /locus_tag="IW833_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1308248.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            6008..7153
                     /gene="dgoD"
                     /locus_tag="IW833_002151"
     CDS             6008..7153
                     /gene="dgoD"
                     /locus_tag="IW833_002151"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAQ1308249.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            7180..7671
                     /locus_tag="IW833_002152"
     CDS             7180..7671
                     /locus_tag="IW833_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308250.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            8114..9313
                     /locus_tag="IW833_002153"
     CDS             8114..9313
                     /locus_tag="IW833_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1308251.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(9808..10566)
                     /locus_tag="IW833_002154"
     CDS             complement(9808..10566)
                     /locus_tag="IW833_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1308252.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            10734..11669
                     /gene="pfkB"
                     /locus_tag="IW833_002155"
     CDS             10734..11669
                     /gene="pfkB"
                     /locus_tag="IW833_002155"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1308253.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            11666..13111
                     /locus_tag="IW833_002156"
     CDS             11666..13111
                     /locus_tag="IW833_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1308254.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            13139..13594
                     /locus_tag="IW833_002157"
     CDS             13139..13594
                     /locus_tag="IW833_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1308255.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            13613..14587
                     /gene="lacD"
                     /locus_tag="IW833_002158"
     CDS             13613..14587
                     /gene="lacD"
                     /locus_tag="IW833_002158"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1308256.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(14698..15259)
                     /locus_tag="IW833_002159"
                     /pseudo
     CDS             complement(14698..15259)
                     /locus_tag="IW833_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            15403..16017
                     /locus_tag="IW833_002160"
     CDS             15403..16017
                     /locus_tag="IW833_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1308257.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
BASE COUNT         5046 a         2677 c         3437 g         4987 t
ORIGIN      
        1 atgactaaat catttacgga agaagtcact tcatttaagg aacgtgagct tcatgaccgt
       61 tatgccgcta tttatatgga cgcaacctat atccctttaa agcggaaaac cgtcgccaag
      121 gaagccattc atatcgtagt tggcattcgc ccggacggtt caaaggaagt gttgagctat
      181 gcgattgcac caactgaatc tatcacgatt tgggaagaga ttttaataga cctccaggaa
      241 cgtggcgtga aaaacgtcct ccttttcatc acggatggct taaaggggat ggcaggggcc
      301 gtgcagcggt tctaccccaa agcccgtttt caacattgct gtgtgcacgt ttcccgtaat
      361 atcagtcaca aagtgcgtgt cgatgatcgt aaggaagtct gtgatgattt taaaatggtg
      421 tatcaagcgt catctaaaaa ggcggcattg gaagcacgtg gtgcttttgc ggaaaaatgg
      481 aaaaccagtt atccaaaaat ggtggaatcg atcctttcga acgattacct gctcactttc
      541 tatgatttcc cattagccat acgcaagact atttactcta cgaacttgat tgaatccttt
      601 aataagcaaa tcaagaaata cagccaccgc aaggaacagt tccaaaatga agagtcgatg
      661 gaacgtttct tagtctcgtc ttttgatact tacaaccaaa aattcctagg tcgcagtcat
      721 aaaggtttcc aacaggccga aggcgagctc gaacaaatgc tgagtcaacc gatggagaat
      781 tagaaacaat ctacagtagg gaagaaccat ttacacaaaa ttattgacgc tcccaaaagc
      841 attgttttta gcagtttaat attattcttt ttcaattaga ctgacttgat ttagttacgg
      901 aatagatact ttaatgtgtt gaagaagcaa cgtcccgtgt ttatttacta aagcatccaa
      961 tattctaccg aatatttaat attcacatta gccctaaaat aattgtcata ccatgttaca
     1021 tttgatataa cagcgttcat tttaaaaata gctgttagtg tgacaaaaca ttaaaccaag
     1081 tgtgacaaaa aacgtgatga aagctttccg gaaacgtatg tgaacaagta tataatgatt
     1141 ttgtcaggag gatagtcaaa atgaataaac gaattcgtga aatactcaaa ctgattatta
     1201 aaaaccctga aatgaaactt tccactttaa caagtgaact tgatttgaca cgtcgtcaaa
     1261 tcaattacgc gatcaatcaa tttaatgaag aattgaaaac aaagaacatt ccgttaattc
     1321 agagaagtca ttctggaaat attacggtac caatcgaggt tatccaaatg atatcacagc
     1381 ctgattacga cataggtgat gatcaggaac ttgtcgttct cactgatagt gaaagacaag
     1441 cattgatagt gatgactctg atcgctaata tcgattacat cagtctggat catttggtag
     1501 atgcggttga tgttagtaag acgactgtta tggaagatat caaacgaacg gaaccgttca
     1561 tcagaaatta tagtctgaca attcgatata atcggatgaa tggttaccaa ttggttggtt
     1621 cggaacaccg tatcttacag ctgttatcgg atctagtaaa acggtttccg atttttcgcc
     1681 gagaaatgat tcgcagaaaa ttggcacaag atgtttctga agaagaaatc gtccatttga
     1741 tccataacat ggaacaaatg ttgcatctta gttattctga tgagtcggta gactatttgc
     1801 agacatcagc gagattttta gtaagccgtg ggatcaagat gtcagataag cacgcgtttt
     1861 ttcccgatga tgtgacggat actcctgaat tccgaatgct gatgatcttg gtacatgaaa
     1921 caggttggag tctatccaaa agttatatgg agtggttcac gttattattt ttaacttcca
     1981 atatttttga gagcaaaact actcaaaatt atgattcgga tcaagaattg cgaaaattga
     2041 tcagccaaat ggttgaaaat ttccaaaatc aaacgttgat tttgatcgat gaccgagaag
     2101 actttgaacg taggatactg agtcatctgc gtccagcctg ttttcgaata cgctataact
     2161 tgagtttagg agtttattca atagatagtc tgatcaagga tagtaatcat gcgattttga
     2221 ttgatttgat gaaagaattg attattccta ttgagaattg gctaaataag gcctttccaa
     2281 atgatgaatt agatctttta tcctattact ttggctatca attaagtaat cataataaat
     2341 tgaataagca acgacaaagg gctgtagttg tttgtactaa tggtgtcatg gtctcaaagt
     2401 tagtacgagc caatatggaa aaactttttc cagagatcca ttttttagct tccctatctg
     2461 tgcgtgattt ctacaaattt gaagctgatt atgatctggt ctttactacg acgccactaa
     2521 aaagttctat gctgcagttt atgatcgagc caatcatgac atatcagcaa caaatcagtt
     2581 tacgatatcg agttttgagt gatttaggaa ttactaaggt tgatcgagca atggatgaat
     2641 tgttaacaat cattaggaaa tatgcaaata tcaccaatcc agtttcgttg cgagaagaac
     2701 tgcagtactt tttgatcaag gaagatggaa gcacgccgct ggacaacttc aatgtattgc
     2761 cttcattgac ttattacttg cggccgagtt atgtacaaat cgttgaagaa gagatgtctt
     2821 gggaacgagc agtggaggtg gcttgccgac cgttattgga acatcaaatc attgactcgc
     2881 gttttatcgc tgattgcttg gatcaaatca gacaaccggg atatgcaggt tacttgggaa
     2941 tgaagacttg cattccccat acaacggtcg ataaaggtgt aatcagtgac ggtgtcagca
     3001 ttttagtttg taaaaagccg atacgatttc ctaatggtga agatgtcagc ttgattttgc
     3061 cgctttcatt ttttgattta acgaagcatc tgcgagccat caatcaaatc gctgatatcg
     3121 ccaaagatga ttcattgatt gagcagttat tgattgctga tgacgaaaag acagtttatc
     3181 aactattaag acaaagatct taaggaggaa aacatgagac tagcagatta tcctaaattg
     3241 acagtgatca tgcgtggtta tacttttgat caggcagatg ctattttaca agcaatgcag
     3301 gaatttgacc atcaatttgc cgtagagatg acaatgaaca caaaaggagc tcttgaaaat
     3361 atacatgaac taaatcaacg ttatggtgac acgacttata tcggcgctgg gacggtaagg
     3421 acattagaac aagcccaagc ctgctatgag gctggtgcaa aatttttatt aggaccacat
     3481 atgttcacaa aagaaatgtt gtcttacgcc aataagaaag gattattggc ggtacctgcc
     3541 gcaatgactc cttcagaagt cgaccgtatg tttaaagaag gagctgatat tgtaaaagta
     3601 tttcctgcag cggttgttac tccacgtttt ttcaaggata ttcaagcacc tttaggtaag
     3661 ttaccactaa tgggggttgg tggaatcagt aaggagaatg cgaaagagtt ttttgaacaa
     3721 ggtgctagct atttaggtct tggttccggt atgttcaaca agcaagacat cgaagagcta
     3781 aatgttaaaa acttagcacg ttcgatgaaa gaattaattg aggctattaa ataggagtga
     3841 ggactttgga actttatttg aatgaggaac tcgtttttcg aaatgttcaa gcaaaaacag
     3901 atagtgaagt cttagcgttt ttagcaagcg agatgtacaa aaaaaactac gtgaaggagg
     3961 aatatattca agcaatccaa gaacgggaaa aagaataccc tactggatta ccatcaacac
     4021 caccaggaat cgcaattccc catgcaaact atgaaatggt caataaaaca actttagcaa
     4081 ttgcgactct taaagatccg gtcctatttc ataatatgga agataacaat gagcagttac
     4141 caatccaaat tgtgatcatg atggcgattg gggaaccaca tgggcaagtt gaaatgttac
     4201 aaaaaattgt tggaatcata caagatgagc cactgcggca agaaatgatc agagcaggca
     4261 atgatactga attattagaa ctattaaaga aagcggtttt ttaatataag gaggaaatca
     4321 taatgaaacg aattttagta gcatgcggaa atggtatcgc cacttcaaca gtagtagcaa
     4381 caaaagttag agagtatttg caacaacatg gcattgatgt ttcaacgact caaacaaaat
     4441 tgatggaagt tccaggaaaa gttcaagact atgatctatt ggtgacgact ggtcaatttg
     4501 atggtcaaac aggtggtgta ccagtaatca aagggatgcc gattttaaca ggtatcggtg
     4561 ccgatcagac aatggaagag attttgaact tactgaaata atagctgatc aaaggagctg
     4621 aaaaaatgaa aatcattatg aacgtcttta acttctttat tgatgctgga ccaacagtta
     4681 tgttgccagt catcatcacg attattggtt tgatttttgg gttaaagatt tctcgtgctt
     4741 ttaaatcagg tttgacatta ggtatcggtt ttgccgggat caagttgata ttggacttta
     4801 tgacgaccaa tgttggacca gctgccaagg cgatggttga tcgaactggt gttaaactag
     4861 atgctttgga tgttggctgg ggttcgattg cggctgttac ttgggcatcg ccaatcattc
     4921 caatcttgat ttttgccatt ctattggtca atatcgtttt gctgattttg aaacgtacac
     4981 atacgctgga tgttgatatc tggaactatc accatatggc aatcgttggt gttatagtgt
     5041 attttgtgac taagaatgtc ttcttaggtg taggtgcatc agtagtgatg gcaattgcga
     5101 cttttaagat ttcagactgg tcgcaaccga tggtagaaag tttcttcgga atccctggtg
     5161 tttctttacc aactgtttct gcgctttcat cactggttat tgcttggccg ttaaattggc
     5221 tgctggatcg tatcccgtta ttcagaaaat caaaattcac catcaaagat gcacaaaaat
     5281 atttgggatt ttttggtgat tctatgatca tggggttagt tattgggatg gtgattggtg
     5341 ttttagccgg ctatgatatc aaagcggttt tacaattagg tgtcagcatg tcagctgttt
     5401 tggttttgat tcctaagatg acttcattat ttatggaagg attgatgcca atttcagatg
     5461 ctgcacaaaa atggtcacaa aaaaaattca aaggtacacg tttatttatc ggattggatg
     5521 ctgcagttgt tgtaggtaat ccagatgtta tcacaacagc attgatcatt attccactaa
     5581 cgattgcatt agctttagta ttgccaggaa accgtgtgtt accattcgca gatttagcgg
     5641 ttgttccttt ccgcgtggca atggtcgttg ccttgactcg tggtaattta ttaaaaaata
     5701 tcattattgg cttagttgtg acagcttcat tgctatggtg tgggtcagct actgcgccaa
     5761 tgctgacagc aattgccaaa tcggttggta ttaacttagg aacttcttca atgttaatct
     5821 catcatttgc tgcgacttcc atgatccaaa gttatttggt atttatcgca tttgcttaca
     5881 aaccaatcat cggtattcca gtgttgttat tagcctttgc gggcgcatgg tattacttcg
     5941 atggtatcaa aaaagttaat catgaaatgg atgaaccatt gaaggcacaa tagtaggagg
     6001 gtttaaattg aaaatttcag atgtcacagt gtataaagta aaaccaagat ggatttttgt
     6061 taagatctcg actgacgaag gaatcgaagg ctggggagaa atgatctcag gaactaaaac
     6121 agaaacggtt gttgctggag cttatgagat aggaaaccgg ctgattggac gtaatccgtt
     6181 tgaaatcgag cgcctcttcc aagaaatgca tcgatctttc ttccgaggcg gtcctattaa
     6241 tggaaccatc gtttcgggtt tagaaatggc tctgtgggat atcaaaggaa aagcgtttaa
     6301 tgttccggtt tatgaattat tgggcggtgc agcacgtgat aaaatcaagg tttattcttg
     6361 gattggtgga gatcgaccaa gtgaagttgc agaacaagca caagatcggt ttgatcgggg
     6421 ctttacggcc gtcaagatga acgcgacaga agagctgcat tatatcgatt cctaccaaaa
     6481 agttgatgaa gtggtggaac gagttgcgtc tattcgcgaa cgttttggtg atgcaatgga
     6541 tattggtgtt gattttcatg gacgggtcca taaaccgatg gcaaaagtgc tagcaaaagc
     6601 tttggagcca ttccatccaa tgtttttgga agaagtcgtt ttacctgaaa atgaagaaca
     6661 ctttaaagaa gtagctgatt ctgtggctgt accattagct actggtgaac gcttgtacac
     6721 tcgttggcaa ttcaagaacc tctttaaaca aggtactgtt gatattatcc aaccagatgt
     6781 ggctctttgt gggggaatct tggaaacccg taaaatcgca gcgatggcgg aagcttacga
     6841 catggctgtt gcgcctcatg cgccatatgg tcctgttgca ctagcagcaa ctctgcaagt
     6901 agactcatgt acgccaaacg tctttatcca agaacaaagc ttagggatcc actataacaa
     6961 aggttttgat ctgttagatt ttgtgaagaa caaagaagtt ttccaatata aagacggcta
     7021 cgtagatcta ccttcaaaac caggtctggg cttggaaatg gatgaagaca aaatcaaaga
     7081 aattgcacaa gaaggtttga tttggaccaa tccgcaatgg aaaaattatg acggtacgat
     7141 cgctgagtgg tgataaatga aaataaagga tgatgaaaga tggcagaagc acttatgatc
     7201 acagtttttt taggactgct gattattttg caaataatcg atcaaaagaa aattatcgtg
     7261 tttgctcaga gtagtcctgt aaggaagagt ttgagtattt tactgacagt cgccatcctg
     7321 ttgattttct ggccggaaca tctgactgat cagattaaac tggttgcttt tgcttctgtc
     7381 attttgatca atggtttttt gaaggagggt ctagcaaaag attctgttgt tagatttgga
     7441 gtattgaacg gtaactttaa tcgatatcct caagtccaag tagaaaaact aggtgacgat
     7501 aaatcattta tttcatttta caaaagcaag aataattctt tttcaatgat ttttgataaa
     7561 gatcaaactg aaatgatgga tgtctttcaa gaacttgata tccattccaa aattacttta
     7621 gatcaaagtt cggaagaaaa aagagcagtt gtaaaaaaag ataatgaatg attctactac
     7681 cgaaattatg atttctttat cacgaataat gaaacttgtt ttaatgaatc atattgattg
     7741 aagtatacaa tgtatattaa gttttggaaa gaaatatatt ggtacaagat atagcaggaa
     7801 tcgattactc gatccctgct ttttttaact tcctttgcag caaaaaaagc tcgtaatcta
     7861 agagatgatg gatagttttt agaatatata tattttcttc aattccagaa aaaaatgacc
     7921 aagctagtat gctaaaaaag tacgatgctt ctatgatctt ttttccgtct ttattaacag
     7981 atgttttaat tgatcaacgc atattgtaat ctaccctaat ttccatagtt cttcaccact
     8041 tttaaaaata gtaaaacccc tacaagtatg ctatagtaaa ttgctaaaga aaaaccaata
     8101 aggtgggaga tgtgtgcgcg atcaaacaag cggaaaaatg attcgttttt attatacaag
     8161 tttgcatttt ctttattgga tgatgttttg ttctgtctat gggtttgcga cattgttttt
     8221 gatttatttc aagattgatc cagctaaaat cggagggatc ttagcattgg tcaatattgt
     8281 ttcaacggtg cttcagcctt ttgtcagtca agtgatcatc aagaaaagaa agatcccact
     8341 gatcaccgtg ctgaagacga tgatagcagg attaggagtg attctcttag ctggggtatt
     8401 gattccgaat ttgccgatca ttacttatat cttgggtggg attttagtag tttctatgca
     8461 atcatttctc aatgcgctgg catttgagta ttcaaatacc ggctacagga tcaactttgg
     8521 cttttcaaga gctggtggtt cattttctta tgctgtaact tcttttttac tgggacagct
     8581 gttagcgatt tattctgccg cgattttgcc aatcttggtt atagttgaag cagtgatatt
     8641 tttcgttatt ttgcatctgc tgccggatgt aaaaaatcaa gaagtttttc ctatgacaaa
     8701 agaacaggat cgctcagttt ctttgagaaa aaaatatcct ttcctgattt tgttattttt
     8761 aggtttcagt tttcttttta ctttccacac catgatcaac agctttttgg cacagatctt
     8821 tgaaaatatc cacggcggca gtaaagaagt tggcatcgca ttgatgatcg ccgctttatg
     8881 cgaattaccg ggaatgatct tttttgaaca gcttgtcaaa aagaaatcca gcaaattttg
     8941 gctgtctgtt gccagtattt catttgctgt cagaggattc atgatgctgc tggccagttc
     9001 gatcctgatg atggaaatga cgcatctcct acaagccttc agtttcgctt tgtttattcc
     9061 agcttcggct tatttgttca atcaatttct ggcggatgat gatcgtgtat ttggacaaac
     9121 gatgatcatc atcgccacaa ctcttggtgg ggtgatagga aatgtcttgg gcggcagtct
     9181 gctgcagcat ttcggtgtat gggagatgct gattttcggt actggttttg cggtgctagg
     9241 tgctttatta actattttgg gcattcgaaa aataccgaag acaggaaacg atagtgattc
     9301 tcttgttgag tagcataaaa atacttagat actagtttta tataaataac atatagtgat
     9361 tattggcgta attacaaaaa acgagaataa cttaagtaag catttctaaa taattgagaa
     9421 acctcatcta aaaagataag aaaatatccc taataaaact tctgtaacat tttagataac
     9481 tgattgtaat ctgaataata aactataagg agacggttag acggtctcct ttttttgcgt
     9541 attataattc tagaattgtt ggaaattgat aataattaac aagaaatatt agacaggaac
     9601 ttcgaactgc ttatcacatt cttattatta tatatttgcg taagtagtcc tggtagtctc
     9661 aatgcactat tctatcttga aactaagaag ttatagaagg agcaatgata gaaagcacga
     9721 gaatacaaat agcgattaaa tatttttcta gacaacctta gttaattcac acagatatat
     9781 tttaggtaca gtcttacaga tatagagtta gtgctgtgtg aatcctgatg ttcgcagtgc
     9841 ataggagtca agtaagattc tagccagtat agaaagtgga agtctggagc gtacctcata
     9901 atctgggaag aaggagcctt ctgatttaaa gccaacaaaa ggaatctcga tatgttccat
     9961 caaacttgaa tgacgattag cagtaagcaa aattgtcccg atctcagctt tataacagtt
    10021 ttcagctgcg gtagtcaatt cctttgtttc accgtttagt gaaacaaaga tcacgatgtc
    10081 atctttggat aattttctac taatgttttt tatgatattg ggatcagtat gcatctcaca
    10141 atatttaccg gtaagttgaa atttgaccat catttcctga gcaatcaatt ctgaaaaacc
    10201 tcgggcaaaa atcatgattc ttctagcagc cttgattcgt tgaatagcat cttcaatatt
    10261 cccgctttct accatattga tggtgcggat tacctcttgc tcatttttaa gaatagcagt
    10321 ctttattcct tcatcaactt tatcaagagc ggcaaaattg atatttgtat tttctttttc
    10381 ttttaacgaa tgtttaaaag acgtaaaccc ctcatatcct tttttcttca tagtacgaac
    10441 gacggttgct gttgaaacgt tggcatattc acttagtttt acaatggaat aggaggggat
    10501 ttcgagtatg tgactttgaa taaaatccca gagatatttc tccgattcac ttaattttgt
    10561 atccatattt tgcctccgat tagttaggat aattctattg taaagcggga gtttccttta
    10621 cttcaagttt atttaatata ttttgtaaaa gttttcaatg aatttagtca tttatgaaaa
    10681 catttacatt cgatcttgat acataatgaa tatgtaagaa aggaggagcg taaatgtcag
    10741 tttatacatg tactttaaac ttggcaatcg acctttttat cgaaacagat gaaatgaaac
    10801 catcccaagt caatcgaaca aacgatgatg atatacaagc taatggaaaa ggtgtgaatg
    10861 tttcattgat catgaagcaa ttaggtatcg atagtacagc tttaggattt aaagcaggtt
    10921 ttaccgggaa ttatattgag gattatttgc atcaaaaaga tattagcact gattttatta
    10981 gcgtaccggg aatgactaga atcaacgttt ttacacaagt taataaaacc aatgaagaat
    11041 tcaaattggt taataaagga cctgagattc ctcaggaaca aatcaaaact tttctaaatc
    11101 aaatacaaaa gctgcaagct gacgattatt tgtgtgtttc tggcagtttg ccgcgagggg
    11161 tctcacccga aattcttatt gatatcagtc gtatttgttt tgaaaataag gtccatctga
    11221 ttattgattc aagctatccg gaaataattg attgtctgcc gtatcagccg ttcttattaa
    11281 aaccaaacga ggaggaattg caatcttggt ataatgtcga gcttagtgat cgtgaaggat
    11341 atattcatta tgctgaacgc ttgattgccg atggagcgaa aaatgttctc ctttctctag
    11401 gaggagaggg aggaatattt ttaaataagg atatcgttta ttctgggaat tctcctaaag
    11461 gtaaagtagt caatacagcg tgtgctggtg acactttgtt gggaacattt cttgcaggat
    11521 atttaaaagg tgaacaggta gatattaatt tgaagaaaag tttagcagct ggaagttcaa
    11581 ccgcttttcg taaaggatta acagattttt cggatgttgc agagttggaa aaacaaatcg
    11641 aaatcaaaga gggagcgagt aaaaaatgac aacttacaaa attgttgccg caacaggatg
    11701 tccaacagga attgcccaca cttatatggc tcaagaagca ttggagcagg cagctaagcg
    11761 caaaggggta tctatcaaag ttgaaaccca tggtcaagta gggatagaga atgaattaac
    11821 accagaagaa ataaaaaacg ctgacgctgt aatcattgca gctgacaaag atgttcaagc
    11881 agaacgtttc gcaggtaagc gtgtaatcga tgtgcctgtc agcaagggga ttaaagatgc
    11941 tgaccaattg atcgaagatg cgttggctgg taaaggttca atcactgcag aaaacaaaac
    12001 agttgacgct gctggtgaga aagcggagat gggggttgca gcaggcattg gtcacagtat
    12061 ttataaaaat ctcatgaacg gtgtttctca tatgctgccg tttgtagtgg caggtggggt
    12121 aattattgca atttcgtttg ccatctgggg tgtgtactct tttgatccca atgatcctac
    12181 ttacaatccg actgccgcta tgttaaaggg tgttggtgat gcggcaatgg gaatgatggt
    12241 tcctatttta gcagcttata tcgctgaagg aatcgctaaa cgtcctggtt tggttgttgg
    12301 gtttgttggc ggactgttag ccaattctgg cggagccggt ttcttaggcg gaatttttgc
    12361 aggtttctta gcaggctact ttatcttact attgcaaaag gtcatgaaag gcttgcctaa
    12421 acaattggat ggtctgaagg cgatcttctt atatccggtg atcggtgtct tgtttgttgg
    12481 tgtagtcatg tctttattag caaatccaat ggaagcagtc aaccaaggaa tgatgaattt
    12541 cttggcagga ttccaaaatt ctaatccatt aattttaggg attatcgttg gctgtatgtc
    12601 agcatttgat atgggaggtc cagtcaataa agcggcttat gttacaggga ctgcgttgtt
    12661 ggcacaaggt aaccatacat ttatggcagg ggtttcggca gcttgtatcg cgcccccatt
    12721 gattactgga tttgccgcac tgttttttgg aaaatatttc gacacgaatg atcgcaacgc
    12781 tggtttagtc aactttatct tgggctcaac gcacattacc gaaggagcaa ttccatttgc
    12841 ggctaaagat ccgctgcgtg ttctgccaac tatgatgcta ggttcatcaa ttgctgctgt
    12901 actgacatat atgtttggtg tccaagttcc cgcacctcat ggaggttttc tagtattacc
    12961 tgttgttact ggtaaggttg catgggtagg gtcgatttta cttggttcaa tcgttggcgg
    13021 agtaatttta gggttgatcc aaaaacgtag tgtagaaaaa cgtgaagcag aagcagctgc
    13081 tgttgaaatg aaagaagcaa ccaacaacta aaggttattc tagaaagaag gatttcaaat
    13141 gactttgatc aaaacaaata aagtttttat gaataaaaat tttacgacgc aagatgaggt
    13201 aatgaacttc cttgcagatc agtcaattgt cttgagcatt agtgaagacc gagatgcagt
    13261 ttacgaaaaa ttgatggaaa gagaagaaga aagcacgact ggtatgatgg atggttttgc
    13321 catccctcat gccaaagatg ctagtatcaa ggatgccgat attgtgattg ttcggttaaa
    13381 cggtaaggtc gattggaaaa gtttagatgg agaaggaacg gattttgtaa tcgctctgtt
    13441 tattccagat tcagaggctg gaacaaccca tctaaaattt ttatctcagg tagccagact
    13501 tttgatgcac aaagatgtga cagaaggtct gaaacaagcc cagtctgctg aagaaatagc
    13561 tgaactttta aaccaaaaat tagaagaaaa ctaaaaagga agtagtgaaa tcatgccttt
    13621 aacagaaaaa aagaaaactt atattcgtca actaagtaat gaaagtggca tcatcagtgc
    13681 gttagcaatc gatcaacgtg gtgccttgaa aaaaatgatc ggtaaatttc aaaataaatc
    13741 agcaaccgat gaacaaatca tcaatttcaa aaaaatcatt tcttctgaat tgactccata
    13801 tgcttcagca atcttattag atccagaata tgggttgccg gcagccgaag ttcgcgataa
    13861 aaatgctggt ctgttgttgg cctatgaaaa aactggttac gatgcttcaa ctccaggacg
    13921 tttaccggat attttatcta tttggtctgt aaaacgtcta aaggagcaag gggccgatgc
    13981 ttgtaagttc ttgttatact acgatgtaga tgaatctgat gacatcaatg accaaaagaa
    14041 ggcgtttatg gagcggattg gttctgaatg tgttgctgaa gatttgccat ttttcttaga
    14101 attggtttct tatgatgcca acgatcctga tacttcttcg aaggaatatg ccttgaaaaa
    14161 accgcataaa gtaaatgaaa tgatgaagga attttctaag cctcgctatg gagttgacgt
    14221 tttgaaagtg gaagttcctg ttaatatgaa ctatgtggat ggttacacta aaaatgaagt
    14281 tgcttatacc aaagaagaag ctgctgctta tttcaaagaa caatccgaga ctaccaacct
    14341 gccatttatt ttcttgagcg caggagtaac tgctgaattg ttccaagaaa ccttgaaatt
    14401 tgcaaaagag gctggctcta cattcaatgg ggtactctgt gggcgcgcaa cttgggcgaa
    14461 tggtgtagaa ccattcgtta ctaaaggtga atatgctgcc aaagaatgga tgcagacaca
    14521 aggacgcaag aacatcgaag agctgaataa tgttttgaaa gaaacagcaa cagctattaa
    14581 attgtaagaa agctttaact ataaccttaa gtttgtaacc taatgatttt taataatcaa
    14641 tttgagcttt taaataaaga attggaagcg tcccgacgct tcctttttcg tttgttttta
    14701 aggcatgata ggttctatgt tgtcgttcaa taagcgcaag aaggtcagca tttttttctt
    14761 agagtatgca aaattgcttt tctacccaat gatttaaaac ggagaggcac ccttggatat
    14821 ggtagatggt gaagtaacgc atttcttctg tgatcacggt ttttttgtct tcttgtagcc
    14881 agatttttaa ataattttct tcgatcagat tatttaatct cttttgaaat tggatatctc
    14941 cgtggttgat gacgaataac ttgtatacga acgtattcgt atagacatag tcgatcaatt
    15001 gagcgtatag atcctcgtag ctatgtgtcg tatcgtagga aatgatttca tccaagtctt
    15061 ggaaaaagct attttccagt ttttcataaa gatcatagat atccgtataa tgtgtataaa
    15121 atgtcgaacg gtgaagatca gcagtttcgg tcagttcacg gatggtgatg ttttgaattt
    15181 ttttatctac taataatttt gataatgctt ctgctaacgc tttttctgtt tttttgacgc
    15241 ggcgatcctc ttttttcatt ttgcagcctc ctaatcaaca taatcaagga attgtgtcgg
    15301 ttatccgaca atttcaccgc tatctgaaca tagataaccg acaccttgtc tattataata
    15361 taagtatagc atatcaatat ttaaagaata ggagaataag tgatggaatt agcctttaag
    15421 caactggaaa agaaagacta caaaaaagcc attcaattcg cgattaaagg tatgcacttt
    15481 gattggtata ccaagaacaa actccttttg aatctttatg gaagatactt ttggtatcta
    15541 gaattgaatc gcgcgacaca aatcattgct ttatatgcgg atgatgagtt ggcaggtgtt
    15601 ttattagcag acatcaaagg aaaatcaaaa aagcatcatt catttagtca aaagttatat
    15661 gttaaactat ttgatttctt acaaaatgca tttgtgaaag acagcaaagg cgggtatgac
    15721 gacaccaatg acgaattgct ttcagactat ttaaaaaaac attccccaga tggagaaatt
    15781 gtctttttag ctgccaaccc tgatttaaag atcaagggga tcggtagtaa attattaaaa
    15841 gaatttgaac gccgagagca aggaaaagaa gtatttctgt tcacggatag cggctgtacg
    15901 tatcaatttt atgaacgacg cggtttcgaa cggaaaaaag aaaaagataa cgtcatgaag
    15961 atccttaata aagaagtaga tctccgctgt atgttatata gtaagatcat tcagtaatgt
    16021 tttttactat cagaaagcag aagtaccacg aaggtcaatg gaaaaagcga gtaattgatc
    16081 catttagatg aagataggtg tagtctcaaa gatattagct ggcaaagata tccccagtta
    16141 aaaatag
//
LOCUS       DACEQH010000061        15396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.062, whole genome shotgun sequence.
ACCESSION   DACEQH010000061 DACEQH010000000
VERSION     DACEQH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.062"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(487..801)
                     /locus_tag="IW833_002161"
     CDS             complement(487..801)
                     /locus_tag="IW833_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ1308258.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(815..1075)
                     /locus_tag="IW833_002162"
     CDS             complement(815..1075)
                     /locus_tag="IW833_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ1308259.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            1245..1559
                     /locus_tag="IW833_002163"
     CDS             1245..1559
                     /locus_tag="IW833_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1308260.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            1561..3216
                     /locus_tag="IW833_002164"
     CDS             1561..3216
                     /locus_tag="IW833_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1308261.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            3299..5434
                     /locus_tag="IW833_002165"
     CDS             3299..5434
                     /locus_tag="IW833_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1308262.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            5609..6118
                     /locus_tag="IW833_002166"
     CDS             5609..6118
                     /locus_tag="IW833_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308263.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            6115..7419
                     /locus_tag="IW833_002167"
     CDS             6115..7419
                     /locus_tag="IW833_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1308264.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFI
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEEISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            7515..8384
                     /locus_tag="IW833_002168"
     CDS             7515..8384
                     /locus_tag="IW833_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1308265.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(8496..8816)
                     /locus_tag="IW833_002169"
     CDS             complement(8496..8816)
                     /locus_tag="IW833_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ1308266.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            8970..9365
                     /gene="dhaM"
                     /locus_tag="IW833_002170"
     CDS             8970..9365
                     /gene="dhaM"
                     /locus_tag="IW833_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ1308267.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            9367..10350
                     /gene="dhaK"
                     /locus_tag="IW833_002171"
     CDS             9367..10350
                     /gene="dhaK"
                     /locus_tag="IW833_002171"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ1308268.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            10378..10983
                     /gene="dhaL"
                     /locus_tag="IW833_002172"
     CDS             10378..10983
                     /gene="dhaL"
                     /locus_tag="IW833_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ1308269.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            11130..11795
                     /locus_tag="IW833_002173"
     CDS             11130..11795
                     /locus_tag="IW833_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308270.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            12078..12737
                     /locus_tag="IW833_002174"
     CDS             12078..12737
                     /locus_tag="IW833_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1308271.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12814..14424)
                     /locus_tag="IW833_002175"
                     /pseudo
     CDS             complement(12814..14424)
                     /locus_tag="IW833_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
     gene            complement(14437..15318)
                     /locus_tag="IW833_002176"
     CDS             complement(14437..15318)
                     /locus_tag="IW833_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1308272.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
BASE COUNT         5170 a         2815 c         3182 g         4229 t
ORIGIN      
        1 aaaaaagggg tgtgacctat gtcgcattcc tttttttgat aaatagactc ccagtagcta
       61 cttctcgata ataagccaac caaaccaaaa acaggagaag ttgtttttga aattatttct
      121 tgtgattcgg agttaaatgg taggctcaca acctcttggt aaacggtgtt caatcagctg
      181 accttcggta ttagctcaaa aaacggcaaa atatgaggag ctattttccc gtttttttct
      241 ctaatactta ggtcaaaccg ctgatttcac aacctcttcc ttaaacggtg ttcaaaagct
      301 tgcatctgcg gtaataagcc caaccaaacg aaaaatatga gaagctattt ttgtttggct
      361 gttcttatta cttgatgcag gacagcgttt tcacaacctt tgattcacgg ttttccagaa
      421 gtagcttctt cgaaaaataa gccgaaattt cttgaagctg atcacttctg tcgcaacctc
      481 tactgactaa ggcttgatat aagcataacc ttgggattgc agttcaacta aatcaaggac
      541 acctgctggt acaacgatta cttgttcagg taaagaactt tctgaaatat gattagcacg
      601 catagcattg gcgcaagcgt gaaaaacgac tccttgcaaa tctaagaatt ctgcattggc
      661 agagtctaga taaccggtaa tcgcgatgcc atttacagat acgacaatcg tcgtttctgg
      721 agaagctttt atcaggtttt ttacgttttt ccctgtttct gaccattttt ctaattcatc
      781 aatatggaaa acgactttca ttgaaatcat tcctttactt tttaataatc atcttgatcg
      841 cttgttctag tttagccgct tgttcttctg gattactatc aggtttctca aagcaatttt
      901 tcagattatc tgcaacgatc atgcccatta cgcgatcaat actagaacga acagcgctta
      961 attgagtgat gacatccata cattcttttt cgtcttcgat cattttttgg ataccacgaa
     1021 tttgtccctc tgtacgtttc aaacggttca ggatattttt attatcaatc tccataacac
     1081 attctcctct atttttataa ttagataata ccccttatcg gtattttatg caaatataaa
     1141 aagtagtgaa aaaaatgaag agtcattctg gttgacatat taataaagct cctgtttaat
     1201 ataccccatg aggtatttat aaaaatgtaa atgaggtgca gaaaatgaat ttgctatttc
     1261 aatcaataaa atcgataaca gtcccagaat taaaggaaaa gcttttggaa aatccagcgt
     1321 tacttgatgt tagaacacct gcagaatacc ggggcgggca tataaaagga gctaaaaatg
     1381 ttccattaca aagtataaac agatatgatg gagacaaaga aaagaccgtc tatgtgattt
     1441 gtcaatcagg gatgaggagt aaacaagcag ctaaagaatt aaagaaatct ggttatgatg
     1501 tagtgaatgt acgtggtggc atgaatcaat ggtttgatcg aacagtagga gggaaataag
     1561 atgaaaatcg ttattgtcgg aggtgtagca ggtggaatgt ctgctgctac acggcttcgc
     1621 cgattaatgg aagatgcaga aatcgttgtt tttgaaaaag gaccgtatgt ttcttttgca
     1681 aattgcggct tgccttatta tctttcggga gaaatcagtg aacgggaaaa tcttcttgtc
     1741 caaacgccag aatcattatc cgctcgtttt tgtttagatg tgcgtccaaa tcatgaagtg
     1801 acagccatct ttcccgaaaa caaaacggta gaagtcgtac atgagggtca aaaacacatt
     1861 gaacagtacg atgcattggt tttatctcct ggtgcaaaac cagttgttcc atcgattcca
     1921 gggataacag aagccgacaa tgttttttct attagaaatg taccagatat cgataaagtg
     1981 atgcatgcat tagaaaaaca gccaaagcgt gccgtgatcg ttggtgcagg attcatcgga
     2041 ttggaaatgg cagaaaacct aaaaagaaga ggtttagaag tcatggtgat cgaacaagca
     2101 ccacatattc ttccaacact ggatgaagaa atggcagctt ttatagaaaa agaattgtct
     2161 catcaaggag tagaagtgat tacttctcat gctgtcgctg gatttgaaga ccacgggaaa
     2221 cgattgcgac tggatgatgg gcgtaccatc cctgctgatt tagttatttt atccattggt
     2281 gttcgtcctg ataaccagct agcagtgacc gctggaatcg aattaggcat acgcgggggt
     2341 atcctagtag acgaacgtta tcaaacgaat attcctgata tttatgcggt gggggatgct
     2401 atcgttgtaa aacagcaaat cactggaaaa gatgcactta tttctcttgc ttcaccagcc
     2461 aatcgtcaag gtagacaagt tgcggacacg atttccggaa tttctcgaag aaatcaaggc
     2521 ggtattggaa cagcaattat acgaacgttt ggaatgactg ccgcatccac cggtttaagt
     2581 gaaagaacag ccaaagaaaa cgaactgtct tttgaagtca ttcatgtatc aggaaaagat
     2641 catgcaagct attatccaga agcaacagat attttactga agttgatctt ccatccagag
     2701 actggcgaga tttatggtgc acaaggtgtt ggggcaaaag gtgtggataa acggatcgat
     2761 attttagcaa cagcaatcaa agggcatttg acgatcttcg atttaccgga attagaattg
     2821 acgtatgcac cgccatttgg ctcagccaaa gatccagtaa acatgctagg atatgcagca
     2881 atgaacattg cagaagggct tagtgaaacc gtacaatggc atgaattgcc gacagaacta
     2941 gcaaaaggaa aaattttatt agatgtgcga acagcagaag aattggaaaa gggcaaattc
     3001 aaggaagcca aacatatccc tttgaatgaa cttcgggacc gattaaatga attagacagc
     3061 caacaagaat atatcgtcag ctgtcatagc ggattacgta gctatctagc ggaaagaatc
     3121 ttgaagcagt ctggctacca cgtaaaaaac cttgatggtg cattttcttt atatcaaact
     3181 gtccgacctg aagaactgat atatcctaac aaatgatact cgttaaaaag cttgcattcg
     3241 actcttaaaa gaggtaaact aatcggttcc accactttaa aaaaagagga gaatgtgtat
     3301 ggtcaacaca caaaaacagt tagaagaagc atatctgaag aacgtgtatc aaaagctaat
     3361 tgataaaaaa aacgaactgc gggaatcgat ggatacggca aaaatagaag gaaaccagtc
     3421 gttgaaagaa atggctggcg agcttcgatt gaattttgac agttacttag ataacctgga
     3481 tactttttcg atgattgaaa tgaaaaatag agaaatcgat cagatgaata tcaaacagaa
     3541 aaacgcagaa aatcagctag caaagatcga acgtttattg aaaagtccct actttggaaa
     3601 agtcgttgta gactttctgg ataatgagcc aaaagaatcc ttttatatcg gtacagcaaa
     3661 tttcacagat gatgcagatg aaaatcttgt ttatgattgg cgttcaccaa ttgccgaact
     3721 tttttacaat aacatgattg gtccatcttc ttatacggtt cgtcaaaata aaatcgatac
     3781 ggttattaaa gaaagacgac aatttctttt ggaaagagat cgtttggtca agttttttga
     3841 tacgtctgta gctattcaag atgacgtatt attagaagtc ttaggccaag atgagaccaa
     3901 tgaaatgaag gctatcactg ctacgatcca atcggaacaa aacacaatca ttcgggatgt
     3961 gaaaagtcaa aatatcttgg taaatggcgt agcaggaagt ggtaaaacct ctgctatcat
     4021 gcagcgtatt gcgtatctgc tctatttata tcgagatacg atgacatccg atgacttgct
     4081 gattctatca cctaatcatc ggtttatcga ctatatttcg aatgtcctac catcattagg
     4141 tgaaagaaat ccactgaatc taaccatcat acagctggtt tcacagcttt cagcagagga
     4201 aatagaaggt gaagaggctt atttcaaacg tatcacggga gaaaatgtat cggagcagac
     4261 agaacgtttg cgtagcaaaa agtttatcga taatctaaaa caatcagatc ctttgttttt
     4321 agatcatccg aattttatcc gtggacttac taaaaatgga aaaacagtcc tttctaaaaa
     4381 aacgatcgaa aaaatatatg agaaagttcc tgctcatcct aaattgatcg ataggcttca
     4441 agcaacgaaa aaggcactga tgagcgaatg gaagaatcat cttttgaaac aagcaaaaag
     4501 tcctgctgtg cagaatcagg tactctcgtt gacagaagat aggcagttgg aattattcgg
     4561 taaattgatt tctgatgatt cggaacaaag tattgtagct tatgcaagga aacttttaca
     4621 aaaaaaatat cgaaaaataa ccagacaaat cgaagaaatg gcatgggtgg atgagcatca
     4681 gttgtttgaa agaatttatg aaaaacgata tggctcagct tatgcttggc aacccacacg
     4741 tactgttgat gaagcagtca ttatattagc tattcgtcat ttacttgttg aaaaggtaaa
     4801 tgtaccagca tttcgctatc tattgattga tgaagtacaa gattataccc tggcacaatt
     4861 aggtctattg atcgaattat ttccaaaaac tcactttact ttagtaggtg acgagaatca
     4921 ggcaatcttt aattcttcca ctacttttgc tgatattatg cgttgtttcg acgactacca
     4981 tctgccgatc catcggtatg atcttagaaa tagttaccgt tctagcggag caatcacaga
     5041 gcttttcaaa acctatgctg tggatcaaga gaagatcgac atcgtatcga tccgaccgca
     5101 aggaaaagaa cccgaatacc gctcttttcg atcctctgaa gaattgttga aaatactagc
     5161 tgagattttg gtttctctaa aaggagaaaa agcggcagtt atcacccaaa cagaagagga
     5221 aacccaaacg ctacaagaat cgattaaaaa aaattcatat ggattagaaa actgccaaat
     5281 cattcctttg agtctagcaa aaggactgga atttgatcat gtaatcttat atccatttga
     5341 aaacgatgga gatgagcaaa gaagacgcag acaaatgtac acggcgatat ctagaggaat
     5401 gaaaagcatt gtcgtattgg aaagagcaac ataattaaga gataaaaaat aaaaagaaca
     5461 actgataaaa agaacaaaat atttttcatt tgctgatgag aaatgttttg ttctttttct
     5521 taggaaaaat ttaggaacac tttatgtgaa aagaaatgaa atgtatggta aactcctacg
     5581 tcgtaacaaa aaaacgtgag gtgaggatat gaaaaaagta acaagagaag aagttgcatc
     5641 aattttggtg aaagataagt atttttcaaa aggtaattac tggttaaaga tttggcaaac
     5701 gttagtggct cttttaggat ggatgatcgt tgttcttcct tttatatggg tctttttccc
     5761 attaacgaga ccagaaagag ccaaggattt ttccttgtac gcttttttat cagaaaagaa
     5821 aatgctgtat ttcttaatcg gcttttttgt attgattttt ttcagtttcc tgattacctc
     5881 atttgtattg actaggtgga acaatcgaaa tctatacaaa aaagtaaata aatcttttga
     5941 atatgaagat gaaaaattga aaaaaagaca agaattactt gaagaagtgt atacggaacg
     6001 ttttggaaca aaagaagaga gagaaacggt ccgtttttat tctgtctccg aagagaaaaa
     6061 tctagatacg acattcattg cagatttgta taaagaaaat ggggtggaac tgaaatgatc
     6121 tcaatcaccc atacaggtaa tttattctta gatacttgtt tgtctattat gtattttttt
     6181 ctcgtttctt atccgatttt aggcggattt gtatggttta ttggtgtctg gtgttatgtg
     6241 tttatataca aacataagca aaaagaatgg gtagatgttc ccttgagtgt cgaaccgttc
     6301 atcacgatca tggtgccagc ccataatgaa gaaattgtga tcgaatatac aatcgagtat
     6361 ttgatgacga aattgaatta tcacaactat gaggtattag tgacagacga tggttcaacg
     6421 gaccaaacac cagaaatatt agctagactg atgaaaaaat acgctaatct tcgtgtggtt
     6481 cggatcgaaa aaaataaagg aaaagcccac gcgtttaata tcggacttgc ctttgcaaaa
     6541 gggaaattga ttttaagcaa tgatgcagac acggtaccgg agccagatgc actgattagg
     6601 tatgtgaatt atttcattcg ccctggtgca agacatattg ctgcggtcac tgcaaatatg
     6661 gatgtccaaa atcgtaccaa attgattgca aaatctcaaa cggttgaatt ctcaagtatc
     6721 gtgggcatca ttaaacgaac acagtcagct gttttcggtg gtctttatgc gtacagtgga
     6781 gcaaatacga tgtatcgaaa agaagccttg atcgatgtgg gcggattcag acaagatcga
     6841 gcgacagaag aaatcagtat tgcttgggat catcagttaa atgactggtt atctgttttt
     6901 gctcctgaga ttatcttttt catggaagtt cctgtgacgt tgaagatgct atatcgacac
     6961 agaaaacgtt gggcaaaagg tggaacagaa gtctggctga cgaatttcaa aaaagtgatg
     7021 ctccatcctt ttaaacatat tggaagaacg atcatcttca ttgatcaaac attgagtatt
     7081 gtctggtcaa tattcttttg tatttccgta gttctttttg ctggattaat cgggcattat
     7141 gtatatcaag gaaactacga acagatttat attactttta cattcagttt tgtgttcatc
     7201 tgctttgaaa tggtcgcagg atttttccag ttgctagctt cgctgattgt agatgatcga
     7261 cgaagaaaat tgaaatacct tcttttcgca ccactatata tgcttttgtt ctggatagtg
     7321 aacgcgataa cgatcgtgac gactttcatt ccagcagtca aaacgatttt aggttatgga
     7381 agcgggacat ggaaaagccc ggaacgtaca aaaaaataaa agatgcaccg ttttttagac
     7441 aaacagcctg ttgtgtttat ggttcttcca aacatttttg cttataataa gaaattagtt
     7501 aaaaagtagt aaggatggga aaagtgatga aaaaaagaat gaagccgtta atctggccaa
     7561 ttgctgtttc cagtattttc ttattgggac agacagcttt tgcggaaact tctgaacctg
     7621 ctcaaacaga accttcaaca accataagct cttctggtgc tgaaaacggt gcctcggaaa
     7681 cagaaatgac ttccaatgta ccgacagata attcaggaga aacgatttca tcttcagaag
     7741 atacatcaat aaatagttct acctcttctg accaaatcga tcatttgcaa gacttggaaa
     7801 ataaaagaaa agaactcgaa gaagcattag aagctgcaaa aaacaaattg aaagaagacc
     7861 aagcagcaat cgatcaagat ttagctgatc ttaaaaataa agtgggaaat gaccgtgcac
     7921 aacttgagaa aaagcttgcc gaagcgaaag caaaagcgga agctgtccaa acagatcttg
     7981 acgggaagat ccaacaagca aaagaattca atcagcaatt aaatcaggtt gctcaaacag
     8041 cttctggaaa gctaagtcaa actcaagcgg atatcacagc ggccagtcaa gcaatccacg
     8101 gaaagatcag cgaagcacaa acagttgcta atgattttga agcaaaaaaa gctgacttag
     8161 cagctagttt ccaacgaata aaagatattt tgcattcggc aggagaaaaa tcagtgacct
     8221 ctggaagcac gacagaaaca gttcagcaaa cgacagagcc atccaaatca acaagtcaga
     8281 ctttgccaaa aacaaatgaa aaaacagctt ctgtcagtta ttccttagca ggggcgggtg
     8341 tgttagtcgc agcagcttac ggaatcatcc gcaaaaagaa ataaaaaaga gaggatggga
     8401 aggtagagta cctcgaagtt agactgaaaa atctaattct tgagtgccac tacatgtccc
     8461 atctctcttt agttattaaa acagatgtcc cctacttatc tcgtggagac agctgttcca
     8521 ctacatgtga ccatcagcat gccattgtcg ctgcctaata cttcatagtc catttttacg
     8581 ccaatgatcg catcagctcc taatgctttt gcacgttcgg ccatttcggc aagtgcttct
     8641 tctcttgcac ccattagttc gttttcatag ctttttgagc ggccgccaaa gatatttcgc
     8701 aaccccgcct ccaagtcttt aaatgcatgg atcccagtaa tgacctcacc aaaaacgatc
     8761 cctttataag ctactacggc ttttccctct acactgttcg ttgttgttgt aatcattcga
     8821 tcactcctct cttttaatta aagtataaca aacttcaaga agcactacac atattttccc
     8881 aaaattagaa atttattata tacttgaatt gttcattaat gaaagcgaaa ccaatcgtta
     8941 gcaactatgt aaaagaaagg aagattcttg tgaaaaaaag tattttgctt atttctcata
     9001 gtcaaaaagt aacagatggt attaaagaaa tgattgagca gatgcaaaaa tccgaagagg
     9061 tgcaaatttt ttctctcggc ggaataaatg gcgagatcgg atcagacccg atgaaaatcg
     9121 tagaagcagt caatgaatca agcgatgcag ctagttattt tgtttttgcg gatatcggta
     9181 gcgcggtatt gaactcggaa ctggcaaaag acatgttgga agaagagcag cagcaacgtt
     9241 atcatctggt ggatgcgcct ttagtggaag gagcttttgc agcagccatc actgccggta
     9301 taacagatga tactcagcaa atcttaacag aagcacaaaa tgccggaaag aaaggatggg
     9361 aataaattga aaaaaatcat gaatgatcca agcaatattg tcgaagaaat gctggaagga
     9421 ttagtaaaaa gctatccaga acttgttcat cgtgtagaat cttcccgtgt tgtagccaag
     9481 aatcagaaag cggaacaagt tggtttggtt tctggtggag gaagcggaca tgaaccatcc
     9541 catgccgggt ttgtagggga agggatgtta agtgcagcgg tgcttggtga tgtgtttact
     9601 tctccaactc cagatcagat tcagacggct attaaggaag ccgatagtgg aaaaggagtt
     9661 ttgctgatcg ttaagaatta tacaggggat gcattgaact tcgatatggc aaaggaatta
     9721 gctgctatgg acgatattga agtagaaagt gtgatcgtag atgatgatat cgccgttgaa
     9781 aatagtacgt atactgctgg aaaacgcgga gttgcaggta ctgtccttgt acacaaaatc
     9841 gtgggcgatg cagcaagaaa cggtgcttcc ctagctgagt tgaaagaact gggagaaaag
     9901 gtcgtacagg caactaagac gatcggtcta gcgcttcgag cagcaactgt accagaagta
     9961 gggaaaccag gattcgaact aggagaagat gagatcgagt atggtgttgg tatccacggt
    10021 gagccaggct atcgtcgaga aaagatgcag ccttctaaag tactggcaaa ggaacttgtt
    10081 actaagattt tagatgatta ctcgaattta ccaaaagaag caggtgtact agtcaacgga
    10141 atgggcggga cgcctttgat ggaacagttc gtgttcatga atgatgtttt agctctgttg
    10201 gaagaaagag gcgtcaatgt agttttccga aaagtcggta attttatgac ctcactagat
    10261 atgcaaggtc tttctctaac actgatcgac ttgacggaga cacaatggaa agattcttta
    10321 gaaagcaatg tacagacgat ttcatggtaa aaaatctcaa gataaaaggg tgataatatg
    10381 aaactaaccg ttcaagaaat ccaagaatgg ctcacgcaat ttgcagaggc aatcaatcag
    10441 aacaaacaat atctcagtga tctagatacc ccgattggcg atggagacca tggaaataac
    10501 atgggaagag gggtttcagc ttatgaagaa gcttttcaag ccgatcatcc tgaaacaatc
    10561 agcgatacat tcaaagtatt ctctatggcg atgatatcga aagtcggtgg ggcttctggt
    10621 ccgttatacg gatctgcatt catgaacatg atgaaagcga caaaagaggt cgattctatt
    10681 gattctcaag aaaaattagg cgaagtgatc gagcagggaa cagcgggtat tcaaagccga
    10741 ggaaaggcag aagctgacga taaaacgatg ttagatgttt ggctgccagt cacagaggca
    10801 ttgaaggcag ggaatttaac aaaagaaatc attgaccagg caaaagaaca caccaaagtc
    10861 ttggtagcta aaaaaggacg agcttcctat ctaggagagc gtgcaatcgg acatatcgac
    10921 ccaggagctg cttctagtgc cattttattt tcaacgcttt tagatgtgat caatcaacct
    10981 taagttcaat aaaactgcgg tgatgctcat cgcagttttt tgtttggctg ttcttatgac
    11041 ttgatgcaga acagcttttt cacaacctct atttgtccat atcgcaaaat tcaggtagaa
    11101 tacaaaagat aacggaaaga ggcgaaacaa tggaacttgg aaaatcagat tatcagttgt
    11161 ttgatcgacc aatctacgca ttcaaacagc tcaaagagag ccatccaaca gataagattg
    11221 aacaaatcaa gaaagaatat aaagaacact ggcaaaaatg gaaagagatt cagctccaga
    11281 cagctgcact tttgcctgat atgtacggca tgagcaaacc taaaatcgaa agctggacga
    11341 atggctggaa tttacgcagt catttttgga gcgcgtaccg aagcgaatct cgtcaagacg
    11401 agaatgcctg tctagcagtt ttgttgaatc aaaaacaata ccaaatctat ttgatgtacc
    11461 aacattataa aagcgaagaa cgatatggta gcattgcagc gtataaccag ttgcttgcga
    11521 tactgaaaga gtggagcaaa acagtcgata tcaaggatta ttacatatgg ccgcaaccag
    11581 aacatgaact tgacgaccac ttagctttgt ctgactattt gtccgataca aagaaacaag
    11641 aagagttgaa aaaagcgatg agagaccgaa cctttcaaat gggtaaacta ttcttctatc
    11701 cgcaagaaat cgaacacgtt gagcaaataa ccgctgaaac cttacaagaa ctagcaccac
    11761 tatatcaaaa gctaggtgcg gaaaaatttc aatagacaac acgaaataag caatgcgaaa
    11821 aacaaacctt actctcggct tgcgagtgaa ggtttgtttt tgattattca aaaagtaaat
    11881 atttaaaaaa ttgataggaa tcaattactt aaggtctcaa aagaaggaaa ataaagcaag
    11941 ggacaaaaag aaatccactc tatttgctgg tatctggttc gcttgacgtt tatgctatga
    12001 taaaagaaaa ttatcagttg gaataaggga agttatgttt ctttattaga aatagctgtt
    12061 tagaaggagg agagattatg agtcatgatc atttatgtgt ttcatttgtt cctttgttca
    12121 atcacttagt acagcaagat caagaaaaag tacacgcact tgtcaaacat cactatgtgg
    12181 aaaaaggaga acagattctt gcgccgaatg gagaacctca attagtgatc gtggcctccg
    12241 gcagtatgaa agtgtatcaa ttatctgcta gcggaaagga acagctttta cgagtaatag
    12301 agccgggcgg atatgaggga gagagccagt tgctaggcgt tccaaatgag tctttatttg
    12361 gcgaagcact agaaaaaacg gaaatttgca tattgagaca aaaagatttc tcggaactct
    12421 tactgcatta tcccgccctt agtctgaaac ttttagcaat caacgttgag aaattaacaa
    12481 aagtacagca gcagacaact tttttgatga tggaaaaagt agaagaacga gtcgctgtct
    12541 atctgcttga cttagctaaa gcgacaggga aggatcactt tttcctgcca atgaaaatga
    12601 aagaacttgc tgcatttatc ggtacgaccc ccgaaacttt atctagaaaa tttaaattat
    12661 tagaggagaa tggttatgta aaaagaaaga atcgagaagt ctggatcatt gataaagagc
    12721 agttagaaga cttgtaaata aaaaaatgac ctccaaatgt tggatttttg acccagattt
    12781 ggaggctagc atgaaattgt acatgctttt ttattatcct tctagtaagt ctcttcgcct
    12841 ataaccaata gaaccaagga cgaacagtgc aaggctgata catgataacg taaggaacac
    12901 ggaaagatca aaagaatcgt ttggcatccg tggaacccag ctagggacag ctgttttgga
    12961 aaaccattct ggtaaatcca aaattccgcc aaagtaattg agtgcaaagg agtaagctaa
    13021 gtagatataa gcaagtttac cgattcgagg aagccatccg aatagaaagc cactaattcc
    13081 gataaacact aaaacggcag gcaggaaatt gaacccggct ttaagaaaca tcaatagatc
    13141 catgtctttc gcatccttca tcgaagccaa agcagaaagc cctagtgcac cagaactaat
    13201 caggattgcc aatatagcaa atagaatagc tagtaacata ttggtccaat aaagttggtt
    13261 cctagaaatt tttgttgcga acagctgatt catacgtccg tgggtttctt ctgtaaataa
    13321 acgattgatg ctagcaattg gtaaaatagc agctagacaa atcaagacga ccatgatcgt
    13381 gcttgtaaag tttttttcaa ttgagacgcc agattggata aacatctgtt tcattaagtc
    13441 gttactttct aaaaactgtt gcatattccc gtagatagag ccgtatgccg cccccatcaa
    13501 accaaaagtg ataagccaac tgattgtcat ccctttattt agatagaata ataaaccacg
    13561 caaggataaa agagaacgtt tagcatacgg ttttccttca cgttcgggga tatagccggt
    13621 gttcaaatct cgatgttcct ctaaaacaaa tgaactgatt atcaagatta gactgaacaa
    13681 aaacaaataa agcaaaggaa ttcagttgtt agttgtaaat ggataggtca aataagacca
    13741 gcctaaaggt ttccacatcg aaagggagga atcagctaca tctgttattg ctcgtagaag
    13801 gtaaagtcca cctaaaaacg ctaaagacag accattcgaa ctggctgaac ttggcatcag
    13861 ttgtgcagca aacagtgcaa cggcactccc caaaatcccc atactaccta cagatagccc
    13921 aaaaagccaa gcaccttctg cagtgatcga atccgcttgg aaaaaaatca tcgtacaact
    13981 gatcgatatt gctaaaagca aattgatcag aatactttca gcgatgacag ctaaagagtt
    14041 tgcttgacgt ccgactcgaa aggaacgaat aagttcaatc agaccaagtt cttctttttt
    14101 tctcgtttgg ctcaccacgt gtaaaacaga gatcacagca gcaaacaaac tgcaaaaaag
    14161 caacatttca tttgcataca tcgctcctaa agtatactca gcagcattcg aagctggagt
    14221 tggtccgacc attgcaatca ttgccgggtt ttgcaaggtt tcgtataatc cagctaatcc
    14281 ttgtccggaa gtcaattctt caaacgcagg gacaaaacca ccagaaaaag cagtgacacc
    14341 gactagccat atcaggatct ttttccagtc acggctaagg ttttgagaaa aaagggtgga
    14401 ccagcgtgaa aatttatcag tcatttcgct tcctcctcat ccttcataat gtctcatgaa
    14461 taaatcctca agagtcggtg gaatagattc aaattttttc acacctaatt tggacgcttc
    14521 agctaaaatc acatcgaaag cttcgttatc agcagcaaat tgagcgaggt gacctttttg
    14581 cacaaagtca tggacaccgt tcaattgtgc taagtgacta ttctctgctt ctgtttctat
    14641 agtgatggtc gaacgagtca aatgacgcaa ttcatcgagt gttccttctt catcgagtgt
    14701 tccttcgcgt atgatcacga ctttatccgc tagtcgctcg acttcactga gaatatgaga
    14761 agaaagtagg atggatttgc cctgttcttt caatttttca acttcttctt ggaaaatcgt
    14821 ttccatcaaa ggatctaaac cagaagtagg ctcatcaaat aaatagagat ccgaatcaac
    14881 agccaaagca gcaatcaagc ccactttttg gcgatttcct tttgaatagt tttttgcttt
    14941 tttcttagga tcaagagaga aacgttgaat cagttcatct tttttttgag cactcccttt
    15001 tccatgaagc ttcatgaaga gatcgatgat cgctcctcca gtcaaatttc cccacaaaga
    15061 gatatcgcct ggaacataag aaagacgttc atggatagat aaactatctt cccatacatc
    15121 ttgcccaaaa atcgttgccg ttccgccgtc tttttttata atccctaaaa gtgtccgaat
    15181 cgtcgtggat tttccagaac catttggtcc gataaatcca acgacttcac ctgaatgtac
    15241 ggtgaaagag acatctttta aagcttcgaa acgaccaaag ctctttttca aatgtgtgac
    15301 tttgacgatt tctgccataa aaaccccact cctttttcta aaaatatata ctaaacaaga
    15361 ttaaatagtt taataaaaag gttttatacc ttttta
//
LOCUS       DACEQH010000062        15347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.063, whole genome shotgun sequence.
ACCESSION   DACEQH010000062 DACEQH010000000
VERSION     DACEQH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.063"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..563)
                     /locus_tag="IW833_002177"
     CDS             complement(<1..563)
                     /locus_tag="IW833_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ1308273.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="IW833_002178"
     CDS             complement(750..1400)
                     /locus_tag="IW833_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308274.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IW833_002179"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IW833_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ1308275.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="IW833_002180"
     CDS             complement(2912..3910)
                     /locus_tag="IW833_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ1308276.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IW833_002181"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IW833_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ1308277.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="IW833_002182"
     CDS             complement(4926..5657)
                     /locus_tag="IW833_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ1308278.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="IW833_002183"
     CDS             complement(5661..6926)
                     /locus_tag="IW833_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ1308279.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="IW833_002184"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="IW833_002184"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ1308280.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="IW833_002185"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="IW833_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ1308281.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="IW833_002186"
     CDS             12268..13770
                     /locus_tag="IW833_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1308282.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETLQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="IW833_002187"
     CDS             13772..14509
                     /locus_tag="IW833_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1308283.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14554..14727)
                     /locus_tag="IW833_002188"
     CDS             complement(14554..14727)
                     /locus_tag="IW833_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1308284.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14703..>15347
                     /locus_tag="IW833_002189"
     CDS             14703..>15347
                     /locus_tag="IW833_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ1308285.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLA"
BASE COUNT         4517 a         3289 c         2591 g         4950 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca ctgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaat aaaaaccaaa
    14521 aatgcacggt cgccatgaag ggaccgtgta tttttatctt cttattttct cacctaatcg
    14581 tatcagccgc atcgcttgtt ttcctgcaca aacaagcaat cgctctacct gttccatcgc
    14641 ataagaaagt accatcggtt cattgatgat atcaagtaca tagtcgatac caccctcact
    14701 taattgagct tgatttttct tctccatatt atgtagttgt gggacaactt tctgacaatc
    14761 agcaattgtg tcaagaacgg atcaatcctt tattggagtt gctcgaaagg gtcttctctt
    14821 attacggaca ggctttgctc actgtacata gacaacaaat cattatccta gtcaatcgga
    14881 tttccagagc ctcattgcta tcattgcttg ataaaatcca gacaaagttt aataaaatgt
    14941 atcagttaca gctaaacttt ggaattgggt cgctttgtta tacagagcaa gagacaccgc
    15001 aatctttcct tcatgccaaa caagtttgcg aatggatagc tttccatcaa tctgtgaacg
    15061 agatccgctt ttttgaagat ttagacctcg ggatcgtttt accagcaatc ccgtcagatc
    15121 aacgaacatt atatgtaaaa cgtattttga aaagcctgac agaagaagaa gtacatttgt
    15181 tcaagaagac tttggcatgc ttcagtaaaa ataacggaag catcaaaaat tgctcagaag
    15241 aattatttat tcataaaaac accttgcaat atcgattgaa caagttccat tctctcactg
    15301 gttatacccc tagaaactat gacgattatc atattctaaa gctggct
//
LOCUS       DACEQH010000063        15316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.064, whole genome shotgun sequence.
ACCESSION   DACEQH010000063 DACEQH010000000
VERSION     DACEQH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.064"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            280..789
                     /locus_tag="IW833_002190"
     CDS             280..789
                     /locus_tag="IW833_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1308286.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            882..2044
                     /locus_tag="IW833_002191"
     CDS             join(882..1158,1158..2044)
                     /locus_tag="IW833_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1308287.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2324..3304
                     /locus_tag="IW833_002192"
     CDS             2324..3304
                     /locus_tag="IW833_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1308288.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3426..4844
                     /locus_tag="IW833_002193"
     CDS             3426..4844
                     /locus_tag="IW833_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ1308289.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4915..6384
                     /locus_tag="IW833_002194"
     CDS             4915..6384
                     /locus_tag="IW833_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1308290.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6394..6861
                     /locus_tag="IW833_002195"
     CDS             6394..6861
                     /locus_tag="IW833_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1308291.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6897..7769
                     /locus_tag="IW833_002196"
     CDS             6897..7769
                     /locus_tag="IW833_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1308292.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7851..10385
                     /locus_tag="IW833_002197"
     CDS             7851..10385
                     /locus_tag="IW833_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1308293.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10541..11314
                     /locus_tag="IW833_002198"
     CDS             10541..11314
                     /locus_tag="IW833_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1308294.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11307..12098
                     /locus_tag="IW833_002199"
     CDS             11307..12098
                     /locus_tag="IW833_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1308295.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12108..12599
                     /locus_tag="IW833_002200"
     CDS             12108..12599
                     /locus_tag="IW833_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1308296.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12617..13027
                     /locus_tag="IW833_002201"
     CDS             12617..13027
                     /locus_tag="IW833_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1308297.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13031..14431
                     /locus_tag="IW833_002202"
     CDS             13031..14431
                     /locus_tag="IW833_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ1308298.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14454..14828
                     /locus_tag="IW833_002203"
     CDS             14454..14828
                     /locus_tag="IW833_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1308299.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5315 a         2156 c         3061 g         4784 t
ORIGIN      
        1 gtttttttat ttgtctcgct taattataca aacggcgtga taaaaaacga gaaatgaatt
       61 ataggtttag acctttatca tttctcgttt tattttattg tatcaaggct ctatcataag
      121 tggtaaaatc tacttttaaa tcacatgtaa tcattgataa gtccagtgtt ttagcggata
      181 atcagctttt tatttatttt ttcttataaa tttgataaat agaaattagt atagccctaa
      241 acaaattatt tgtgttttat tgtttagggc tatactatat tggtacaagg aatagaaaga
      301 ggtgtttatg tggttgataa aattcagttt aatcaagtta tagaagaatt catcaaagta
      361 aataatttaa tgacccatat tcaaaaacga ccaatcagaa taactaatga tctaaaactc
      421 agtacgagta tgattcattt gattgatgtg attggaaatt atcctgaaac tagtattacg
      481 gaattagctg atcgattagg cgttaccaaa ggagctgtat cccaacagat tcccacttta
      541 gagaaaatga atcttataag catttctcaa aaaaaaggaa ataagaaaac taaacttgta
      601 tcgttaacaa aatatggtcg aaaggtgtac gaatcacata attatttaca tgaagagctt
      661 tatagctcaa taaatgaagc gttagaatca ttctcaccag accaaatttc aaccattaaa
      721 cagattctag aaaaagtatc ttatacaatc aatgaatatc aagtaagtct gacaagaaag
      781 gaagattgac atgaaaaaaa tgatttttga atcggctaca ctagactaga cagaaaaaat
      841 aaggtgtgta aaataaatga aaacacactg gaggaatttt tatgtcaaga cgtcaacgta
      901 gaacctattc aaaagaattc aaacaacaag tcgtcgatct ttatctcgca ggaaagcccc
      961 gagctgaaat cattcgagat tatgagctga ctacctcggc ctttgataag tgggtcaagc
     1021 aagcgcaaac tactggctcg tttcaggaaa aagataactt gacccctgaa caagtagaac
     1081 taattgcatt acgaaagaaa aataaacagc ttgaaatgga gaatgacatt ttaaagcaag
     1141 cggcgctgat attgggacga aaagacaagt aattgatacc aataagcaca aatactcaat
     1201 atcagcgatg tgtaaagtgt taaacatctc ttgccaaacc tattattatc aagccaaacc
     1261 agttgaagat gaatcagaac tggaagaact cgttcaagaa gaatttattc gcaatcgaaa
     1321 agcttatggt accaggaagc tgaagcagtg cttggccaat ctaggtcttc aagtgagtca
     1381 acgcagaatc ggccgtatga tgaaacagcg tggcttgcgt tcaacataca cattggctca
     1441 tttcaaattc cattcttcta cttgtaacga agccaagacc gccaatattc tagacggaac
     1501 cttcctacag gaaaagtcat tagaagcgat tgtaaccgac cttacctatg ttcgtgttgg
     1561 gaagaaatgg cagtatatct gcctgattct tgatttgttt aatcgagaaa tcatcggcta
     1621 ttcatgcggg gagaagaaag atgcgacgtt agtcaaggaa gcttttgcaa agattccgta
     1681 ctcgttgaat aatgtacaac tttttcacac ggatcgtgga aaagaattcg ataaccagac
     1741 gattgacgaa attttatggg cctttaatat cacgcgttcc ttaagcaaaa aaggctgccc
     1801 ctatgataac gccgttgcag aatccaccta taaatctgtc aaagtagaat tcgttcatca
     1861 ataccgcttt gagacattgg ctaatttgcg attagagcta tttgattacg tccattggtg
     1921 gaataccatc cggctgcacg gcacgttagg ctatgagaca ccgatcagtt ttcgacagca
     1981 gagattggcg cagcgaatcc ttgataatga gctcggatgt gataccggtg gcgaggcacc
     2041 ttaattattt gaatgagctt ctgccttaga gaatcagatt cgaggaggct cgttgtcaag
     2101 ggcagtcgga acaccagcag caagcttacg caatacctta taatttttgt caaaaaaagt
     2161 gttgccattc cacatatatc tttaaacagc tattgccaga taaaaataaa tgatattata
     2221 tatataaata cttatccgaa aaggggaatg agagagtcaa ggttcttgga atgtcgaata
     2281 gtcgagtagg tagattttct atctacaacc ttgttgttta aaaatgaaaa aaattggaat
     2341 tagagaagtt gcgaaagagg caaatgtttc tcctacgact gtgtcgaggg tattgaatga
     2401 tcgtggatat ttgagtgagg aaacaaaaag aaaagttttt gatgccatga aaaaattaga
     2461 atactatcct aatgaactgg cgagagcact ttttaaaaac gaaacatata ccgtaggttt
     2521 gatttttcct acaattacta atccatttca cgctgagtta attcaagata tcgagttcga
     2581 gctttccagc aggggatata aagtgttgct gtgcaatagc ttaaacaatc caacaaaaga
     2641 aaaagagtac ttgacgatgt tgagaaagaa tcaggttgat ggaattatag taggaacgca
     2701 caataaagat attgcggatt atgatattcc agggctacct attgtagcga ttgatagaag
     2761 tcttggtgaa aatacagtta cagtatcttg tgataattac aggggtgggg aattagctac
     2821 tcaactactt gtaaatcatg gctgtcagaa tatactgtgt attcggggag atagtaagat
     2881 aaagctaccc gcgaatagta ggactttagc atataaagat gttttgaata aattcatgct
     2941 ggaaccgtta gtcctagaag taccatttgt aaaagaaatc aatgaaaaaa atgaaataat
     3001 taagacatat ctggaaactc atccaaatat tgacggggtt tttgcaggag atgatttatt
     3061 ggcttctttg gcgattaaca acttaggaag ttcgggcaaa agagttccaa aagaggttaa
     3121 agtgattgga tttgatggag ctcaacaaac attgatttat aatccagaat tatcaactat
     3181 tcaacagcct atagatcaaa tatctaaagt agcagtaaaa aaattattga atatgattaa
     3241 gggcgaaaag gagacggatg aactttcact tccagtaaag ctcataaaaa aaaatacagc
     3301 ataagttaaa tcggcgaaaa attccttcgt cgatttaaaa atatttttat gaaacgggtt
     3361 gacatatgaa acgggttgac atatattagt tgtgaagata aaaacctttt cactggagga
     3421 agaaaatgga gaaattatta attaaaagca ataatcatca actttactac caacctaagg
     3481 atgtttgggt cggagatgtt atgccttacg ggaaagaagg aaaattcttt atttatcatc
     3541 aacgtgatac tagaaatcca gttccttttg gagaaccgtt tggctgggct ttagcaacta
     3601 cgaaagattt tgttcatttt gaagactttg gggaatctct tttaagaggg gatgacaatg
     3661 agagagacca gtttatctac gctggttctg tatttgaggc aaatggaaag gttcacgcat
     3721 tttatacagg gtataatcgt gaattcttag cgcagggaaa gacatctcaa gtgttgcttc
     3781 atgctgagag tgatgatttt gttcattggg aaaaatcaga aacagcattg gagttagaac
     3841 cacaagaagg gtatgatatt cgaaactgga gagatccatt tgtcgtttgg gatgatgaaa
     3901 aagaagaata tttgctaatt ttaggggcgc gaaagggtca ggataaacat ctacaaaccg
     3961 gacgtttagt ggaatttaca tcaaaagatt taactaattg ggaattcaaa ggtgattttt
     4021 gggctccggg attgtatacc atgtttgaaa tgccagaact tttcaagatt ggtgactggt
     4081 ggtatcttgt ctattcagaa tactcagatg gaaataaaat actctatcga atgagtcaaa
     4141 gtttggaagg tccttggatt aagcctaaag atgaagcttt tgatggacgg gcttattatg
     4201 caggtagaac agcttatgat ggaaaacgtc gagtcctatt tggatgggta ccaacaaaag
     4261 atcaagaaaa tgataaaaac aattatttat ggggaggtac tttggttcct catgaagtct
     4321 atcaaaaaga ggatttttca cttggggtaa aacccataga cgaagtgtgg aatgcattca
     4381 taaatttaca tgatattgcc gatgtagaga tagaaacagc agacacaaga gaagaagtaa
     4441 cgcttgtcaa aaatagtgga actttgttta atttagaaac aacaatttca tttgatgaga
     4501 atcttagtga cttttcactg agattatata aaaaccttga gacagatgaa tcttacgaat
     4561 atcgcttcaa tttagatgag gggcgtctta catttgataa aaacccttgt tacccttggt
     4621 ttcaatatat ggataaagga cttattagac ctatatcttt aaagcctaac caatcttata
     4681 aactaaaagt gattgttgat gacaatataa tgacacttta tattgatgga atcgctctta
     4741 acgctcgtgt acatggttgt tttggtcaag caattactat tacagcaaca aatggatcaa
     4801 tgactgcaat aaataccaag ttttcaaatg aaataaagaa ataataaacg tcaatcatgt
     4861 ggcaggtctg aaaatcaaac atgacctgtc acatttaaaa gggaggataa atgaatggac
     4921 tacaaaaaaa ttgccgaaga tgtattggaa aacgttggag gaaaagagaa tatctcaagc
     4981 gctacacact gcattactcg tttacgttta attcttaaag acaaaagcaa agcgaatatg
     5041 gaaatgcttg aggaaataga aggtgcaaaa ggtgttcttt tcaatagtgg acagttacaa
     5101 gtaattttcg gaacaggtat aattgaaaag gtttacaaag agttcgttga attgacagga
     5161 gctaaggaag tatccgtttc tgaaatgaaa gacgaaggag tcaataattc aggaaataga
     5221 cttcaacgag cttttaaagt tttctcggat atctttattc cgatcattcc agcttttatt
     5281 ggggcagcaa tggttttagg aattcgttcg ttgattacca ctcctggaat gtttggaatg
     5341 gatggctcat tcgctgatca caatgtattc ctaaatgatt tatccaaatt tctgggtatt
     5401 attgcaacca catttgatta tttaccagtt ttagttatgt attctgcaac aaaacgtttt
     5461 ggcggaaacc ctattttagg attacttgta ggatttgtta tgattcatcc aggactagcc
     5521 aatcggaatg atttcgtcat gggaactata aagcctgatt attggcattt atttggatta
     5581 agcattccag cagttgcgtt tcaagggggt gtttttccag caattttgac ttcttggttt
     5641 ttagcaaaag ttgagaaaat tacaaataaa tattctcctc aagtacttgc ttacatttta
     5701 gtcccaactg tgacaattgt tcttgccaac tttgcactat ttttagtgtt tggtccattg
     5761 gggaatttta tcggtaatgt tttaggtgga atcattgatt tcctatatat gaagatgggt
     5821 gcctttggtg cttttgtttt tgcaggcgcg ttacaaccat tagttgtaac aggaacacaa
     5881 catgctattc aaggtattga ggcaaactta gtcgcaacaa ctggattcaa ttatatccaa
     5941 cctatttggt cagtttcaat tattgcccaa ggtggcggtt gtattggtat gtatttactc
     6001 tttaagaaaa aatctaaaga tagagatatt gcgatttcaa gctttattcc aacactggta
     6061 ggtattactg aaccggctat ctttgctgtt aatttgaaat attcaatcgt tccttttgtt
     6121 tgctcgttta ttggtgctgg ttttggtgga gcatttatga aaatttttga tgtaaaagcc
     6181 attggacaag gattgactgt attgccaggt ctgactattg ctaatccagt tcttccttat
     6241 atatttggta atctcatagc attcatttta ccaatcattt ttattctcgc ttatgataaa
     6301 tataaagtgt ttatccctga agctaaacac cgtaaatttg cagaaaaaac tgactcaaaa
     6361 aaattgccag ttcaagtaaa gtgagatgag agcatgttaa atttttttag aaaaaatgaa
     6421 ataagcgttg tagcacctgc aaccggtgtt tttgaaaaac tggagaaatt aagtgatcca
     6481 gtcttttcaa aaggaatgat gggacaagga tttgcaattg atgcaaaaaa cgaaacgatt
     6541 gtatctccaa tcgatggttt tgtttcaagt atttttccta caaaacatgc cttaggaata
     6601 aaaacaaaat caggaattga gatacttgtt catgtaggaa tagatacagt tgagctaaat
     6661 ggtgagggat ttgatattaa agttcaagaa ggacaaaaag ttaaggctgg ggatattctt
     6721 atgaccatta attttgtagt ttttgaaaag aatcacaaga ctaaagatgt cattgttgtt
     6781 tttccagact accaagacga aattttcatt ccgaaacttg aagaagtaaa acaaggacaa
     6841 gaattgtttg ttcttggcta atgataagta attaataata gaatcgagga aaatgtatgg
     6901 tttttggggc tattgaagct ggtggaacaa aatttgtttg tgccattgga gataaagatt
     6961 tgaacatatt tgaaaaagtc agctttccca cgactacacc caaagaaaca atgaatttag
     7021 ttttagattt ttttaatcat cataaagatg atttagaagg tatcggagtg ggatcgttcg
     7081 gtcctattga tattcattgt gaatctaaaa cgtatggctt cattacatca acgccaaaac
     7141 ttgcttggca ggattttgat tttattggaa gtttaaaaaa gcaacttaat attccaactg
     7201 cttggacgac tgatgtaaat gcctcttgct atggtgaata tgtttctgga aatggcagag
     7261 gcctttcaag tgttgtttac tataccgtag gaacgggcat aggaggtggc gctatccaaa
     7321 acggaaactt cattgaaggt tttagtcatc cagaaatggg acatatgctt gtaaaacgac
     7381 acaataaaga caacttttct gggacatgtc cattccacgg agactgttta gagggcttag
     7441 ctgctggtcc ggcaatcgaa agaagatcag ggaaaaaggg acaagaagtg actgaaaatg
     7501 acgttacttg ggaaatagaa gcttattaca ttgctcagtg tgcatataat acaacattga
     7561 tgttatcacc tgatgtcatt atatttggtg gcggagtaat gaaacaaaaa cacttaaaaa
     7621 agaaagtaca agataagttt attgagttga tcaacggata tgtcgctatt cccgatgttg
     7681 acaattatat tatgacacca aaattgttag ataattctgg tattgtaggt tgtttagctt
     7741 tagcaagaga cgttaaagaa aaatcttaat ttggaaaaaa gatcgtctcg actatttgtt
     7801 tggttgatgc gatctttttt ttcaattagt aattgaaggg acgcctaaaa gtgaaaaaaa
     7861 aaatgaaaga tgaagtatta gagtatatca aaaataattt aaagtattac gatttttctg
     7921 ctcaagatat tgcaatgaaa ttttgtatta aaagaaatgt cgcttcccac tatttgaatc
     7981 agttattttc agatggaaaa ttactaaaga atgattctgt tagaccagta atgttcaagt
     8041 ataatcaaca aaagccaaaa gactgctttt ctaagtttat tggagcagac atatctttga
     8101 aatccactat agataaatgc aaagctactg taatgtatcc ccctaatggc ctcccattaa
     8161 taataaaagg aaacagtggc gttggaaaaa gttttttagc aagtttgatc tatcagtatg
     8221 ctcttgatag aaaagtaatt cataatgacg caaaatttgt tgttgtgaat tgtgcagatt
     8281 atgcaaataa tccagagcta ttgtcagcag ttttatttgg atataaaaaa ggagccttta
     8341 ctggggctga aaaagatact gcagggatgc tatcttctgc taatggagga tacctttttt
     8401 tagatgaggt tcacaatcta agtgcagaaa accaagagaa attgtttctt cttatggatt
     8461 ctcaaaagta tcgtaaactt ggtgaaagtg tgcaatggga gtatgcaaat gttcgcctta
     8521 tcttagccac aacagaagat aaaaatacct ctttgttagc aacttttaga agacgcatcc
     8581 cagctgaaat cactctgcca gattacaata atagaagtac atctgaaaaa attcaattat
     8641 tatttgaatt tcttaaagat gaagcaatta caattgattc aaaaatattt tgttcgattg
     8701 aactttttac tgatctttta tcgaaaacat ttgaaggaaa tgttggagaa ttgaggaatg
     8761 aaatcaagct tctttgtgca gaaggttatt taggaaataa gagaaagtct tcaatttatt
     8821 taggggacaa actagacagc ggtttttgga tagatccaga aattagtata gatttaaaaa
     8881 aaatgtttct taaatcactt tctgaaattg atttgattga actttttcaa aatcacattt
     8941 caattgaaga aagcatgagt caattaagag atagaatact taaagagtgt gcaggttctt
     9001 ctgagtataa ttatttagaa agtgacgaat ttatagcatt aagaaactat gttgttaata
     9061 aaatatcacc aattattgat tcgacaggtc tatgtttatt agatgaattc ttggcggata
     9121 tcagtctatt tataatgttt attgatgtaa ttgaagaaac atcaagaaat tttttgctgt
     9181 ctaagttcag aaaatttcgt atgaaaaatg ataaacaaaa attattggca aatgagattt
     9241 ggagtagttt agaagtcgaa gaaagtaaac aagaactttt gttgaaatgg atactttttc
     9301 ttgtaaagaa gttctatgtg aaaataccag aaacccattg tctgatagtt atgcacggta
     9361 aaataacagc ttctgcgatc gcaagggaaa caaataagtt gctgaataca tatgtttatg
     9421 aggctttcga tatgccaatt gaaggtgaga ctagtgactt aattaaccta atcaacaatt
     9481 tttgtaaaag tatagatacg acgaatggac tcattctgct agttgatatg ggatcattgg
     9541 aacaaatgta tgaaaaaatt gaatccaatg taataggtga tttggtaatt ttaaacaatg
     9601 tttctacagc tttagcgatt gaatgtggga ttcaagtttg ccaaaaaaag ccaattagtc
     9661 atttttacca gatggatttt gattcgtttc aagttaaagt tcagtattat aaaggattat
     9721 cacaaaagaa aaatgtaata gtatcttgtc tctctggtga ggggatttct gaaaaagtta
     9781 aggatatttt aaaacggtat ctcgataatc aagtggagat tttgattttt gattttaacg
     9841 cattaaagaa aatagcaaag gaaaaagata ctatgatttt taagaataca atttgtgttc
     9901 tttcaaccac tgaattttac attcaaggaa tcgattgttt aaatttagaa aatctaatta
     9961 atgggaatca aaccttagaa aaattaaata agtatatgga ctcggatagc tgtgagttct
    10021 gcttaaatga attagttaaa ctctttacta ttgaaggcgc tagtactaag cttcgctttt
    10081 tagattccaa aaaagtattc aatgagatag aaaaagtact atatttgtat gaaaaatatt
    10141 acgatgtcaa aatcccaagt tttctgagaa ttaatctatt tctacatctt tctggaatga
    10201 ttgaacgaat tatgattggt gatggaataa caaatgaaca tttgaaagag ggagcgcaac
    10261 tatttgatac ttttttaaat gtatcaaaag attttttttc agaaatcaaa gatatttact
    10321 gtattgaaat acccaaagga gagtatgagt taatctatac aatttttgag cagactattt
    10381 tctaaatagc atactagcgt ctaaaaaaat gaaagggtgt ccaaagtata tttagacgct
    10441 catttaatta atatttactg tattttcccg ctttattgga taatgttagt tggcacagta
    10501 attgcttata taaaagtata agaagaaagg agagaacaaa atggcgtttt ggcaaacatt
    10561 tttagttgtg ttgtatggat ttttcattaa ctgggataag gaagggccca aaatcggaac
    10621 ctcccaacct gtcgttgcag gtttcttgac cggacttatt atgggagata ttcaaactgg
    10681 actttatatc ggtgggactt tgcaattaat gacactaggt attactagtt ttggtggtgc
    10741 atcagtaccg gattatcaaa cagcagcatt gatcggtact tatttagcaa tctcaactaa
    10801 ccaggatgct tctataggaa taactttagc aataccggta gcaatgttaa ttgtacaact
    10861 agatgttcta aagtggtcaa caaacatttt ttttcaacaa aaggcagaaa aattcgcaca
    10921 aaaagaagat tatcaaaaat tagagtggat gcaatatttg gcatcaatta atacaagctt
    10981 tgtatctgga attcctgttt tgttaactgt gatattaggc ccaacgttag tgggaagtgc
    11041 aatcaattat attcctgaat gggttaccgg tggtttaaag gtagcagggg gactattacc
    11101 tgctgttggg ataggaatgc tacttaaata tttacctaca aaagattact ttgcctattt
    11161 gttagttggt ttcgttttgg cagtttattt ccaaatacca attttaggga tttcgttact
    11221 tggcgctgca atcgcactaa tcgtatataa aaatagatca aataatactc aagtagcacc
    11281 agctgcaaca ggaggttttg acgaagatga ataagaaact gacaaaaatc gacaaacaag
    11341 taattttccg gtggattctc aatggtgcaa cttcattaaa ttatgaaaaa atggaagggt
    11401 tgggttatgc gtattctatg cttcctttta tcaaggacac atataaagat gatcctgaag
    11461 gaatgaaaca atccgtttta gctcacctac aattttttaa cactacgccg tatacagcgc
    11521 cttacattat tggtatgaat gttggaattg aaaaagaaga aaaaaataag tctttggaag
    11581 cagtaagtgc tctgaaaaca ggtctaatgg gtcctttagc gggaattgga gatagtgtat
    11641 ttgtagttat tccatggact atttttggag caattgcagc taatatggca ttggaaggta
    11701 gttttgttgg aattattttg tggatactgg catcaatttt attaaggatt actgcttatc
    11761 ctttatacct tgctgggaaa gaatcgggtg cgagcctact aactcaaatt gaaacaaaat
    11821 taaagtctat tactgatgca gtatcaattt tagggttaat ggtggtcggt gcactcattg
    11881 ccacagttgt aaaagtgaat gttggctttg aatttaaaca aggcgactta acaatgaccg
    11941 gaaatgaaat ctttgatcaa gttatgcctg gtttattacc tgcatgtgta gtagcaattg
    12001 tgtattattt gttaggacga aaggttaaac cagtttatgt aattctatta atcttagtag
    12061 tttctattgc gttaagagcg ttaaatattt tgaagtaggt gttgaatatg gatgaaaaaa
    12121 tgaaaggtat tatacatgtt cgtattgatg atcgaatgat tcatggacaa gtagcaacac
    12181 aatggacaaa tcaattgagt gctaacagaa ttatggttat taataatgaa gtagccaatg
    12241 atgacgtaaa aaaagctgta gttcgcctcg ctgcaccacc taatgttaga acttcaataa
    12301 ttacaaggga agtagcagtt aaaaatattc tttctggtaa atatgaagga caaaaagttc
    12361 ttattgttgc agtttctcca atcgatgtta aatttttgat tgataacggt ttaccaataa
    12421 caagtgtaaa cgtaggaaat ttatcaagaa ggaatggaac agaacgcatc cgtccgacga
    12481 taaatattac tgaagaagag agagaggctt ttaaagaact aattagtgat ggagttgaag
    12541 tgacagtgat acaaactcct agagattcaa aagaattttt aaaggatttt atttattaag
    12601 aacagaggat gagtggatgg agtttataat tacatctcat ggaaatattg ccattggatt
    12661 tgtagataca ttaaaaatgt tttttggcga taatttaaat tgtcatgtgg taacacttgg
    12721 agatgcaggc attgaagaat tccgtgaaaa cattgaaaaa atagtttctc aagtaaaaaa
    12781 tcaagaagtt atggtatttg ctgacttgat tggtggaaca cctttcaacg aatttgctaa
    12841 aagaagtgaa gtatttcaaa atgggtttca acttcttggt ggcatgaatt taggaatttt
    12901 gattgaggct gtcaatttga gattacaagg aaaaaaaagc gatgatatca tcgaaaatct
    12961 aagaaatatg aatacaattg cgtgtcttgc agatatgcaa agacatacta acgaagagga
    13021 tgagtgataa atgcaaaaaa ttttttacaa gcctaaagat ttttggtttg gggattgcat
    13081 gcctttttat aaagatggaa ctttttatct atttcatcaa cgagatacga gaaatcctaa
    13141 acctttaact gatccttttg ggtggtcact agttacaacc actgattttg tgaaatttga
    13201 agaatacggg gaagtaattc atcgtggtgg caacgatgac attgatcaat acatttacgc
    13261 tggaagtatt gtccaaggaa tcaatgagta tttagctttg tatactggac ataatcgcaa
    13321 agctaagttg gatggaaaaa cttcagaagt gttaatgatt gcaagttcta aagatttaat
    13381 tcattggaaa aaggatggcg aagcagcaaa acttgttcct caagaaggat atgataagaa
    13441 tgattggcgc gatccttttg caatttttga tcaagatcat ggaaaatacg ttttgatact
    13501 aggagctaga aaaccagcaa gtaagaaaaa gccgacgggg agattggtct attttgaatc
    13561 cgaagattta aaagattgga atttcaaagg agactactgg gctaataatg aatttaacat
    13621 gattgaaatg cctgatatat ttgaaattga tggaacgtgg tatttacttt tttcagagta
    13681 ttgtgaagat aaaaagacta aatatcgagt cggagcagga ttattttcag attggaaatc
    13741 accagtagac gaagctttcg acggaaaagc ctattatgca gcaagaacag tcggaactaa
    13801 tgaaactggc cgttttctaa ctggctgggt ggcaacaaaa gaaggggaga acgatcaaaa
    13861 taactgggat tggggcggtg cacttgttcc acatcaaatt attcaaaaga cagacaaaac
    13921 tcttggtgtt aaactgcctg aaaaaataat tgagaatttt aaagaaatca ctccattttc
    13981 aagttttaag ttagagaaag aatttggaaa aaaagaaaga gtaattgttc ctaaaatcga
    14041 agattatttt ctatttgaaa caactttgac tattacatcg ttaacaaagg agtttgcttt
    14101 aaaattattt gtagatagtg atactcaaga aggatacgaa tttcgtattt cattgattaa
    14161 taatcagctt actttcggaa aaactccgaa ctatccttgg cctcaaatgt ttgataaggg
    14221 gctgcaacga ccactatttt tggaaatagg caaagaatat gaactaaagt taattgttga
    14281 tgatacgata gctgttttat atattgataa tgttgcttta agtactagaa tgtatgataa
    14341 agtcggaaca gcattaagta tgactgttac ggagggagca atcaaggtag ataaactaga
    14401 ctattcaata aattatgaat gtggatttta aaacaattga agggatgatg taagtgaaat
    14461 taaatggttt acaacatatt ggaattccaa caaaagattt tggagcttca aaaatctttt
    14521 atgaatcctt agggtttagt ctgattaatg aagaaatgaa tgtgggaagt aaagtcggct
    14581 ttttcgagtt gaatggaacg ataatagaaa tatgggaaga taaaactact gagtgtagag
    14641 gagccattga tcatctagca ttagatacgg atgacataga tgaggtattc aattcaattc
    14701 aagatttagg atatgaatta ttagataaaa atattatgga attaccgttc tgggaaaatg
    14761 gaatccgtta ctttaatttc tacggaccaa atcatgagat tatagagata agccaaatga
    14821 agaaataaat agataacgaa gtgctctaag ttggtatgaa cgaaaagtac ttcatttttt
    14881 ggtatcagca tttcaatagt ttaatgagaa tttactattt caaattaatt caaagtgaca
    14941 gtatttttta cttatttggt ttcaaatttt gtaatccttc ctatttattg gaatacactc
    15001 cacattttgt atacaagtca tatcatgttt tagcaaagct cttaccaagc aaagttataa
    15061 tgatgttttc caaagtttga tctttcgatg gaggctaaca catatttttt gcaggacagc
    15121 caaaattaga tgtatagaat agagtctgtg aggcgtatag acagtttatt gtctgtatgc
    15181 ctttttttat tttgaaaaat ttttagtatt tttcgaaaaa tgctcactcc tagagtaagc
    15241 gccccataga acagtacctt actaacaaat gcacctcaag atatacttga gctatcaaga
    15301 agtctggagg ttttct
//
LOCUS       DACEQH010000064        15173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.065, whole genome shotgun sequence.
ACCESSION   DACEQH010000064 DACEQH010000000
VERSION     DACEQH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.065"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            146..688
                     /locus_tag="IW833_002204"
     CDS             146..688
                     /locus_tag="IW833_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1308300.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            705..1790
                     /locus_tag="IW833_002205"
     CDS             705..1790
                     /locus_tag="IW833_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1308301.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            1802..2608
                     /locus_tag="IW833_002206"
     CDS             1802..2608
                     /locus_tag="IW833_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1308302.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            2609..3436
                     /locus_tag="IW833_002207"
     CDS             2609..3436
                     /locus_tag="IW833_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1308303.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            3433..4506
                     /locus_tag="IW833_002208"
     CDS             3433..4506
                     /locus_tag="IW833_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1308304.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            4934..7630
                     /locus_tag="IW833_002209"
     CDS             4934..7630
                     /locus_tag="IW833_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1308305.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            7707..8195
                     /locus_tag="IW833_002210"
     CDS             7707..8195
                     /locus_tag="IW833_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1308306.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            8207..8701
                     /locus_tag="IW833_002211"
     CDS             8207..8701
                     /locus_tag="IW833_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1308307.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            8722..9531
                     /locus_tag="IW833_002212"
     CDS             8722..9531
                     /locus_tag="IW833_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1308308.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            9528..10349
                     /locus_tag="IW833_002213"
     CDS             9528..10349
                     /locus_tag="IW833_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1308309.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            10400..12499
                     /locus_tag="IW833_002214"
     CDS             10400..12499
                     /locus_tag="IW833_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ1308310.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            12496..13614
                     /locus_tag="IW833_002215"
     CDS             12496..13614
                     /locus_tag="IW833_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ1308311.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            13653..14531
                     /locus_tag="IW833_002216"
     CDS             13653..14531
                     /locus_tag="IW833_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1308312.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            14722..14874
                     /locus_tag="IW833_002217"
     CDS             14722..14874
                     /locus_tag="IW833_002217"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308313.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         4729 a         2472 c         3326 g         4646 t
ORIGIN      
        1 aactagaatt tattccaaaa gataaagaaa tattcagcaa aaaaaatttt acaaagggtt
       61 gcctttttca ataaatttcg tatactaata cgagtggtag taaacaggaa gtttactatt
      121 agaaaacttc gattcggagg aaattatgga aattggtgag aagttaagaa atttgcgcat
      181 ccaaaagaat ttgacacaag aagaactagg cgaacgcaca gatttgtcaa aagggtatat
      241 ttcccagttg gaacgagatt taagttcacc atcaatggaa acattctttt ctattcttga
      301 agtcttaggt gtgactccag aagaattttt tcatcaagaa gcagcgaatc tgcaagtggt
      361 ttattcaaaa gaagatcata cggtttattt cgatgaagaa aacgggtatg aattggaatg
      421 gctcgtggct gattcgaatg aaaaagaaat ggagcctgtc ttgctgactt ttgaaggtgc
      481 aggagaatat aagacgtttg aaccttcatt atctgaaacg tttatttttg tattagaagg
      541 ggaattagag ttaagcttag gagagacggt ctatgtggca aaagcaggac agtcgatcta
      601 ttatcaagcg acaaaacaac atcaattaag gaatcatctc aaaaaacgaa caaaagtatt
      661 gattgttgca acgcaatcat atttgtaata tagggggtaa aaacgtgggg aaaccaatca
      721 tttcatttga tcaagtagtg aaacgctatg atgatgaaac agtattaaaa aaagtaagtt
      781 ttgagatcga acaaggaaaa ttttatacac tgcttggtcc ttccggctgt ggtaaaacaa
      841 cgatcctacg tattatagct ggattcaccg aagctacaga aggggacatc tattttgaag
      901 ggaaacggtt gaatgatttg ccagctaata agcgacaagt aaatacagtc ttccaagatt
      961 atgcactttt tcctcatatg aatgtttttg ataacgttgc ttttggattg aagatcaaga
     1021 aaatgtccaa atcagatatc gaaaagaagg taaaagatgc tttgcgtatg gtccagcttc
     1081 ctggatacga aaaacgggag attagtgaaa tgtctggtgg tcaaagacag cgtgtagcga
     1141 ttgcacgagc aattgtaaat gaaccgaaag tattgctatt agatgaaccg ttatcggcgc
     1201 ttgatttgaa actacgtaca gatatgcagt atgaactgcg ggatctgcaa caacgattag
     1261 gaatcacgtt tatttttgtt actcatgacc aagaagaagc gttggcaatg agtgatgaga
     1321 tttttgtcat gaataaaggg aaaatcgtcc aaagtggtac acccgtagat atttatgatg
     1381 agccaatcaa tcatttcgtt gctgattttg ttggggagag caatatcgtg gacggtgtaa
     1441 tgatcgaaga taatctagtt gagtttgttg gtaagcaatt tgaatgtgtc gatggcggga
     1501 tgcgtctaaa tgaaccagtt gaagttgttt tacgcccaga ggatttgacg atcacgacac
     1561 cagataaagg aaaactagtc gtaacggtcg atactcagct gtttcgtggt gtccattatg
     1621 aaatcatctg ttatgatgaa cagcaaaatg aatggatggt ccattcaacg aaaaaagcta
     1681 aagaaggaag caaagtagga ttggcctttg agccagaaga tatccatgtc atgcgtttca
     1741 acgagtctga agaagaattc gatgcacgtc ttgacagcta cgaagaatag gggggacaag
     1801 aatgaaaaca atgcgcagga tctattccgt cccttatatt ttgtggctcg gtttattcgt
     1861 gatcgcgcct gtattaatga tcatttatca atcttttttt gatatgaatg ggcaattcac
     1921 gctgaataat tatctatctt attttacgtc ggggacatcg ctttctatga cgatcaattc
     1981 tgtatggtat gcatttttga tcacgttttt cacgctgatc atcagttacc cgactgctta
     2041 ttttttaaca aaactgaagc acaagcaatt atggctgatg ctgattattt tgcctacatg
     2101 ggtgaatctg ctgcttaaag cttacgcatt tattgggatt ttcagtgtgc acggcagcgt
     2161 gaatcatttc tttgatttta tcgggatcgg gccgaaacag atattattca cagacttcag
     2221 ttttctgttc gtggcaacgt atatcgagat tccatttatg attatgccaa ttttcaatgc
     2281 attggaagag atgaatcctt ctttgatcag tgctagtaga gatttaggcg cgaatagctt
     2341 tgagaccttc cgacgagtga tttttccgtt atcattaaat ggtgtgaaaa gtggtgtcca
     2401 agcagtattt attccttctc tttctttatt catgctgact cgtctgattg gcggaaatcg
     2461 ggtgatcact ttaggaacgg ctatcgaaca gcatttcttg gttacacaaa actgggggat
     2521 gggttctaca atcggtgtga ttttaatcat tgctatgttc atcgtgatgc tcctgacagg
     2581 cgagaagaaa aaaggaggtc gctcataaat gaaaaaattt aaatggtcgt atctttattt
     2641 agggatcgtc tttttattct tatatgcacc gatcttctat ctgatttttt attccttcaa
     2701 tgaaggagga acgatgaaca gctttacagg atttacttgg gaaaattatg aagctgtttt
     2761 tgaagataca cgtttgatca ttatcgtatt gaatacgttc atgctggctt tcttatctgc
     2821 attgatcgcc acgatcatcg ggacatttgg ggctatggga atctattaca cgaaaaaacg
     2881 acgtgcacgg acagcgttgt tgagcatgaa taacatctta ttagtctctc ctgatgttat
     2941 tatcggtgct agtttcttga ttttctttac actactagga ttcggattag gttttcagag
     3001 tgtattgctt tcccatattg ctttcagcat tccaatcgtc gtcttgatgg tcttgccaaa
     3061 attgcaggaa atgaacgatt caatggtcga tgcggctaga gaccttggtg ctaataattt
     3121 ccaagtagtc aaaaatatca ttttgcctta tctttctcca ggaatcatag ctggatattt
     3181 tatggcattt acctattctc ttgatgattt tgcggttaca tttttcgtca caggaaatgg
     3241 ctttacgacg ctctcagtag agatctattc tcgtgcaaga caaggaatca gtttagaaat
     3301 caatgcgcta agtgcgttag ttttcttgtt ctccatgata ttggtcattg gatactactt
     3361 tattagtaaa gaaaaccgtc caaaacggct gaagaaaaac agaagagtac gcgagggggt
     3421 ggcacggctg cgatgaaaaa attacaatct ctgtttttag gtattctcat cattattgcg
     3481 gtgctgctgc ttggtgttcg tcaacttgaa aaaacaagtg gcatgagtgg agcaaaggta
     3541 cttaccatct ataactgggg agattatatc gatcctagtt tgatcacgaa atttgaaaaa
     3601 gaatatggat acaaagtgaa ttatgaaaca tttgattcta atgaagcgat gttcacaaaa
     3661 atccagcaag gtgggacaaa ctacgacatc gctataccaa gtgaatatat gatccaaaaa
     3721 atgatcaaag aaaaactagt acttccatta gatcattcga agatcaaagg attaaataat
     3781 attgatccac gatttttaga cttggatttt gatccagata atcagtattc gattccatat
     3841 ttttggggaa ctttagggat cgtatataac gataaattct ttgatgcaaa tcaaattaag
     3901 cattgggatg atttgtggaa gccggaatta aaagacagtt tgatgctgat tgacggtgca
     3961 cgagaagtta tggggctttc tttgaatagt ttaggctatt ctttgaatag taagaatatg
     4021 gcacagctga cagaagcagc aaataaattg aatcatttaa caccaaatgt caaagcaatc
     4081 gtggcagatg agatcaaaat gtatatgatc aatgaagaag cctccgtggc agtgacattt
     4141 tctggcgaag ctgccgatat gatgtgggaa aatgaacatt tacattatgt gatcccacca
     4201 gaaggatcca atctttggtt tgataatatc gttatgccaa aaaccgtgaa aaataaagaa
     4261 ggtgcctatg atttcatcaa ctttatgcta gaaccggaaa atgcggcaca aaatgcagag
     4321 tatattggtt attctacacc aaatgaagaa gcaaaagcgt tattgccaaa agacatttcg
     4381 tcggacgaac aattctatcc atctgatgac acgatctccc atcttgaagt ctacaaagat
     4441 ctaggatcga aatacctagg catctataat gacctattct tagaattcaa gatgtatagg
     4501 agataatggc aaaactcagt aagaatgggg acttatcaac gttctaaaga gctaaaaatc
     4561 gtcctttttc tatctgagtt ttccaatctt ctaccaaaag cggatgaaac gccgcttttt
     4621 ttgtgttatt ttattttttc taccacgttt ttctaccaga gaaaagtgga caaaaccatt
     4681 gtaaaatagg gcccgagacg ggctagtaaa tctaagattg aaaaagttac agaaggataa
     4741 agtaattgga aactatgcaa atatacttaa cccttctgta atctattaat aaaactaata
     4801 tatgaatcca gttcaatatt tgtagctatg aaaaaacata aaaatcatat tagataaaaa
     4861 ctaaattggt agataaatta ttttgtcaga tatctgaggc aaatattcga ctatttgtgt
     4921 ttcaaagtaa aaattggaat acaaatatag aatagacata ttgcagccga tagtttatta
     4981 tgcttttaga cacattaaac tggaggaaaa aatgaaaaag agcgctaaag atcgagtttt
     5041 tgatgagctg atacgactaa cagggaatat gtcttctgtc actgccgaga aaatttcgaa
     5101 gaatctaggt atctctcgtc agaatgccag ccattatttg acgagattag tggaagataa
     5161 aaaggtcgag aaattaccgg gaaagcctgt tttgtggaag cctttagatg aatatgcggt
     5221 tgtagataat acggagcaaa taaacgaagc ctttcattct gtagttggtc atgacggaag
     5281 tctgagagag gtcattcaaa aatgtatagc cgctgtcaaa tatccgccta acggactcag
     5341 tgtattaata aatggagcca cgggtgttgg aaaaagtttt ttagccacaa aaatttttga
     5401 atacgctatc catgagcaaa ttattgaaaa agatgcacca tttgctatct taaactgtgc
     5461 cgattatgcg gacaatcccg aactgctttc ggcaacactg tttggataca agaagggggc
     5521 ctttacgggg gctgaaaagg ataccgatgg tttattggca acggccaaca acggctatct
     5581 ttttctcgat gaggtccatc gcctatcgaa agaaaatcaa gaaaagctct ttctatttat
     5641 tgatactgga aactatcgtc cagtcggtga aaatgtaaat tggcattctg ctaaggttcg
     5701 ttttattttc gccacgacgg aaaaaggtga aaattattta ttagacacct ttgatcggcg
     5761 tattcaaatt agtgttatgt tgccaacttt tgaagatcgc cccattcgag aacgattaga
     5821 gctgattcaa ttattttttc aaaatgaagc ggatgtttta caaaaagata ttattgtttc
     5881 aagagaagcc ttgtctttaa tgttatctca tccctttagt gggaatattg gtaagctgaa
     5941 gaatatcatt aaaatcagtt gtgcggatgt gtatagtcga acacaagaag aaccattaac
     6001 gattgggaaa aatgagatct tgattcagtt aaatatgctg gaatccgaag tagaaagctt
     6061 gccgattgct agtttattga taaagaaaaa tcaaaagata aaaacggatc gaatcaaacc
     6121 gtttgattat gattcgattt tggaaaaaat tactcagctg ataaatgaag gtaatttcaa
     6181 tgaaaatttt tcttcgataa agaatgaagt acagcatttg gtgcatcaca ttgataagga
     6241 ttctttttta tcaatgagta gaaaattgat gattcagatg tttagaaagg tctgggtatc
     6301 cgttttagga aaaacgtacg gattgacgac gagtggacct gttgcagaaa ttattagcag
     6361 atcctatgtc atttccccaa gctataagac agacttaacc gggataaatc gtacattagc
     6421 tgcaaatcta cctcggacga cttacttatg taatcagttc ttggagcatt taccaccgct
     6481 ctcagaaaag gacgagaact ttctaagact gcttattgtc gtgactttat ctgattatgt
     6541 agatgaatcc atcgagctga agggcctgtt gcttgctcac ggagatagta cggctagcag
     6601 cattcaagca gtagtgaatc aattatgcgg caattatgtt tttgaagcgt tggatatgcc
     6661 agtagagagt aatgtgtcag agattgtcga atcgacaagg aatttcgtga aacggcaagt
     6721 gaatacagat agtctagtgc tgatcgtaga tatgggctct ttaaatcaaa tttacacaca
     6781 gatcaagaac gatttaaaag gggagttact tcttctgaat aatttgacga cttccattgc
     6841 acttgatatt ggcttgaaga tgacgatgaa tgcgccattt aaacagattg cggaggatgc
     6901 agcagacaag tattcaatca atgttcaata ctttgaaggc ttttctcaaa gtagcaacat
     6961 tatcatttcc tgtatgtcgg gtttggggat ttcggacaaa ttgaaagatg tttttcgtca
     7021 tcatttatcg gatgaatcca tcgaagtttt cacgaaggac tacagagaac tacgtgaatt
     7081 aatcgaccaa aatgatgaaa cttattttga caagacaaaa ttggtcatca cgacttcgga
     7141 tcttccacgt tccttttcaa ttcctaatat taatgtttat gacatcttag acggtgatgg
     7201 agcagccaat ttggagcata ttttgagtcc tgaaatgaag ccggcagaat ttaaccgctt
     7261 gattcaggag ctagtaaaat tcttttcgtt ggaaggaatt gcggaccgtt tgaactttct
     7321 gaacccaacg atcgtgatga gtgaagtgga aacagtcatt tctaaatatg aaaattacta
     7381 tcatttcact ttaaatggcc gtgtaaaact aaatctctat atgcatacgg cattgatgat
     7441 ggaacgttta ttcttggctc ggacagaaaa atctgaagaa ccgaattatg atttgaaacc
     7501 agatgaagat gaattttatt atgtttctag aagtattttt caatcaatgg agatgaaata
     7561 taattttaaa gtcaatgctt atgagctttc gctgctctat gaattattag gaccgtatat
     7621 tcaaaaataa agtgtctaaa aaagtgtcta atctgttttt tttaggttag acactttttt
     7681 taatgtgaat taacgtgatt aaaccaatgt ttttaaaagt tggcacagga attgcttata
     7741 taaaagtgaa cagaaagaaa ggaattaacc atatgaagaa aattctaata gcgactcacg
     7801 gtcacttagc ttcaggcttt cttagcagta ttcaattact gacggggaag gttcaagaga
     7861 ttacggtgat taatgcttat gtggatgagt gtgattttga acaggagttg aatgtatttg
     7921 taaaccaggt gaacgagcag gatcagattt ttgtttttac cgatttattt ggcggaagtg
     7981 tcaatcagaa gattaccaaa acctttttgg agaaagagat tgctgtaact attttgtctg
     8041 gctttaatct gccaattctg ctcgaaatag tattggcaac caaggaatta acaaaggagg
     8101 atattcagga actggtaaat aaatgccaac aggaattaaa gatgaccatt cttgatgagg
     8161 tacatgtagt aaatgaagag gaagattttt tttaggagga agaaaaatga ttaaagcagt
     8221 gcgaattgat gaacgattaa ttcatggaca agtagcgatg acttggacaa agagtctaaa
     8281 tttaaccggg ttagtagtag caagcgatga ggcggctacc aacgatattc aaaagatgac
     8341 attgaagatg gctgcaccat cgaatgtcaa agtcattatt aaaacagttg atgacgcgat
     8401 tcatttgtta aaagatccgc gggcgaagga catgcgcttg atggttttag tccctaatgt
     8461 caaagatgcg gtaaaagttg cgaaagcttt cccaacggaa atcgaattaa tgaacgtggg
     8521 gaatgctggg aaaatgacac cgccatcgga agataagaaa accttatcaa aggaagtgat
     8581 gttaacgacg tcagaaattc aagacttaaa agaattagtt gaactttatc cagataccgt
     8641 ttttcagcct acaccggcga tggaaaaacg ttcagctatc tctattttga gtgattttta
     8701 gaaaaaggaa ggagaaaaat tatgtatgat gcatttgtag tcgctttggc cgtttttatt
     8761 ggagttgctg gacatgaaat gttcgggatg tccatgctga gtcgtccgat tgttgttgct
     8821 ccgttagcag gagcgttgat gggggatttg cagacggggt tgattgtcgg ggcgtcacta
     8881 gaagccatct ttatgggtgt cgtcaatatc ggaatctcta gtacggcgga gccttctttg
     8941 gctgctgggc tggctacagc gttcgcaatc aaaatgggag gaaacgtgga tgcgattatt
     9001 cccattgtct ttccactagc agtattagga ttacagttaa tgaatatgat tttttctttt
     9061 gtatgtggac cgatggctgc taaatttttc aaatttgcaa aatctggtga tcaaaaagga
     9121 atcatcaatc tgcacttctt tatgtggttt gtccactatg cgctttatgc tcttattcca
     9181 ttctttgccg ttttatttgg agcagatgtt gtcaaaagcg tcttggataa tattccagag
     9241 gtcatcatga acggattaac agtcgctgga aatctgctgc cggcagttgg gatggcgatg
     9301 ctgttacgga tgctttggag taaaaatatt gctgtgtggt ttttctttgg ggccgttgtt
     9361 atggcgtatc tcgagttacc attgattgca attgccgcag ttggaacgat tattgcaatt
     9421 gttatagccc aaagagatct acaactaaaa aatatatctg ttcatgcagt tcaaagcgat
     9481 gctgctgtaa atgcaacgct tgaccaagaa gaggaggatt tctttcaatg aaattagctg
     9541 atggaatttc taaagatgag aaaaaattgg ttcggaagat gttttggcgc tcgatgacta
     9601 tgtatgcttc ggtgaatccg gtgacgatgg gcggcggtgg tttcgcctat tctatgatgc
     9661 ccttcattaa tcatttctac aaaagtgaag aagatcgagc tgcggcgctg gaacgtcata
     9721 cacagtattt ttcgacgacc attccttgcg cgtcctttgt catggggatt gcggcatcga
     9781 tggagaagga aaattctgaa aaagctggtt ttgacgtgga aagtatcaac gcgatcaaaa
     9841 cgagtttgat gggaccattg gctggaattg gcgattcatt gttctgggga gtttggcgtg
     9901 tcgttaccgc gggactagcg atcaacttag ccaatgccgg aaatattttg gcaccaatta
     9961 tctttttagt tatgttcaat atacctaatt acttgatgcg atactatggc ggcgttttag
    10021 gctattcatt aggctctaaa tatattgaaa agctgtatgc gaacgggtta attgaaattc
    10081 tgacaaaagc tgctagtatc ttgggtctga tcatggtagg tgcaatgacg atcagtatgg
    10141 tcaaattcaa taccgttttg agcttttcaa tgaagggcga agaactaatg aatgtccaag
    10201 aaatcctcga tagtattttt aaaggaattg taccgttatt ggtaacattg agctgtttca
    10261 aattactaga taaaaaagtc ggcgtcatca aaattatttt tggaatggtc gtcttgggaa
    10321 ttttattgtc attacttgga atcgcatagt aggaagctgc agctactagg ctgcagtttt
    10381 tttagcaagg agggaaaaga tgactattaa tgaagtaatt tattcaccca aacacccatt
    10441 ttttaatgct gagttaaacg acggaaatgt gactacttca aacgaataca gctactatcg
    10501 gaatattcct gatgtaaagg gattgacctt cgatgtaagt aatcaaaaga cattagcaaa
    10561 ccttgatgtt tgcgggacga ttcaaacgtt gaccttttat cgagaaaatt tactaactga
    10621 ggaaaaacca ggtgtttggg tcaataaaca gctctctcaa accaattcgt tatccttaaa
    10681 ggttgaagta aacgggattg tctaccattt gtcagaatcg gatcatcgta ttgaagtcga
    10741 tgtgattcaa gactgtttgc ctagatatat tcatcattat tcgacgttta aggtcattgt
    10801 agtatccttt gctcccatac tggctaataa gcgtttaagt atgcttgttc aacaggttta
    10861 cattgtgaat gaaacacacg aaagccttca agtttcagtg aaagatgtgc cttcgtatca
    10921 agagaagtat tcggatcagc aaaatgtgtt gatttctcaa aaaggaaata aggacacgat
    10981 cgcacaacag gaagtggcaa gcttcgccat tgcttttgtg gacccaaatg cttttgaaga
    11041 agtaatgact tttcaggaat ctgatatcga aaaatggctg aaggaaacat tgaactattt
    11101 tgctcagcta tttgggcagc tgtcaatgcc aaacgataga gttgtccatc tctatcgtcg
    11161 tgcactttat caaagctttt ctagtttcgg tatgaatcga cagggacagg tcgtcggcag
    11221 caattggggc agctatcctg ctactaatcg aatttggaat aaggatatgt attacgcatc
    11281 attgccattc accatgtttg aaccggagtt atgtcaaaaa acggttctat ggtttgatca
    11341 atatggcgtt aagtttcctg gttcaaaatt tacaggaggt atcaatcatt ctctgagtaa
    11401 ttcgttatcc agtgcgttac tggctagtct ttattttgaa catactgggg atacaaaatt
    11461 ctttgagcgg tgtccgcagg ttctactgaa tgcttcaaga attattgagg acatattagc
    11521 tcaacgtcgg tcaggggaac caatgctttt tccatctgtt tggttatcag acgcctttgc
    11581 acttggaaag tatcataccg gcagcaatct ttgtctttgg aaggcctgta ttggacttgc
    11641 agaaattttt gaagccctag aacaactgag tcttagcaag cgatacaaaa acattgcctg
    11701 taaactaaag gaatcaattt gtaaacaaat gactatcgat ggtccttttg gtgagcaatt
    11761 tttggaggga atcggtgatg aggagaaaac aacctattca gtgaagcact atcaaaaacc
    11821 gattcttgag caagggttaa tctttttaag tgatgtgatt gtggatggaa aaattaattt
    11881 attgatgcat gatggagaag aatcagacac tacgttgatg cctttttatc aatttttaga
    11941 taataaagat catttatatc agaacaccat ggccttttct gctagttctt tcaatccaac
    12001 ctatagcgag gaaatcaaag gcatcacttg gggattagaa tccggcgcca cttttcctgg
    12061 ctttatcact gttttaatga gcgagctgca caaccctcaa gctttcgtaa cccgattaga
    12121 agaactaatt tgtttggcag atttagacgg ttcgtggtgg tggtggccgt atcgcttaga
    12181 ggctcaacaa ggagaagtcg tgcgagactt tggctgcggc aaatgcggct gggcatcggg
    12241 tctttttgtc agcctaatga tcagtcaata tttcggattg aaattcaaaa aaggagtatt
    12301 gcaaattgat cctgttgata atttaacgtt ttcttggaag aatttgaagt tcggtcaagc
    12361 cttcatttcg attgaatgta cgcaatccga gttgagtatt tcaaatttgt cagaaaagcc
    12421 attgaaaata agtgatacga aaaaaattct tcacgtagaa aagggaaaaa cgattcacat
    12481 tcaaaggaga aaaagatgag tatactaaaa aatattaccc aatttaaaaa aatccagagt
    12541 cgagcaatca ctcccgaaaa tccaacaggc gagaaaggta aagctgcgat gttgccaagc
    12601 gagctaggac caagccgcaa aggtcgtggc gcaattccgc tgacttctgg tgaagaaact
    12661 gtgattgcgg atattactgg aacgggaata attcgtcata tgtggatgac gattcgtgat
    12721 cgaacagaat ttggcagttt tgttttacgt gatttaatct taagaattta ttgggatggg
    12781 tcggatagcc ctgctgttga aagtccttta ggggatttct tttgtaatgg gtttggagag
    12841 cgttgcgagg tgaactcgtt gccgatagtg gttaatccta cagggggctt taattcctat
    12901 ttcgaaatgc ctttccaaac atccgctaaa attacgatcg agaatttaca tccgaaggat
    12961 atgtcctcat ttttttatac gataaattac gctttggtgg acgaattgga agaaaatagc
    13021 ctatattttc acgcaaattg gcatcgggaa cgcattacca aattacagga ggattataag
    13081 ttaattgatc gattaaaggg tcaaggctat tacgtgggta cttaccttgc gcttacctgc
    13141 ctagaacgtt attggtgggg cgaaggcgag tttaaattct acattgatgg ggatagtgag
    13201 tatccgaccg taacctcaac aggttcagaa gattattttg gcggggcgtg ggcctttcat
    13261 gagtacgatg agttcggacg tccacatgcg aaaaactatt caacagcctt tttagggtat
    13321 ccttaccaat caaatagaga tgccacaaga gatcgttttg aaacgggaaa attgaatgcc
    13381 gttcatgcct ttggtgacga cgcaatgcca cgacatggtc tttatcgttg gcatcttcaa
    13441 gaccccatct gttttaccga agatttatcg gtcactttac aacaaatcgg caacgatgat
    13501 gttcgcttat ttgagcggca agacgatgtt gctagtgtcg cttactggta ttcaacagaa
    13561 gcttcaggaa atactaaacg tcatttcact gatcgagaac gcttgccgcg ttaatgaagt
    13621 gtttttcaca tattcgaagg gaggaaccta tgatgatata tggggcaatt gaagctggag
    13681 ggactaaatt tgtctgtgca gtcggtactg aggaattaac gattttggaa agagtcagtt
    13741 ttcccacaac aactccagtt gaaacaatga agcaggtcat tgattttttt aaacctttta
    13801 aggaacaatt ggcaggcatc ggggtcggct gctttggtcc aattgatatt caatgcaaat
    13861 caacgagttt cggtcatatc acatcaacac caaaacttgc ttggcaaaat ttcaattttg
    13921 taggaacgct aaagcaatat ttcaatattc cgattgaatg gacaacagat gtcaatgcgg
    13981 cttgctatgg tgaatatgtt gctggttctg gcaaagggtt agcaggtgtt gcatacttca
    14041 ctgtgggaac aggtatcgga ggtggtgcct tgttcaatgg gactttcgtg gaagggttca
    14101 gtcatccgga aatggggcat atgttgatca agcgacatcc aaaggacaat ttttcaggaa
    14161 actgtccctt ccaccaagat tgtttagagg ggatggcagc aggaccagcg attgaaaaac
    14221 ggttgggtgt aaaaggccag aatttattgg ctgacgattc gttttggcag attgaagcct
    14281 tttaccttgc ccaatgtgca tacaatacaa cactgatgtt ttcgccagat agaattattt
    14341 ttggcggtgg tgtaatgaaa caagaacaca tgaagaaaaa agttcaggat aaatttgttg
    14401 agcttataaa tggatatgtt gaaataccac caattgatag ctacattatt acgcccgaat
    14461 taggtgataa tgccggaata atcggcggat tagcattggc cagaaaagcg gtaagaaata
    14521 agcaaccata attaatgaat gtttcattga tactcatgtc aaaagtattt aaccgagtaa
    14581 tcaaatgatc aaatgaagtt ggataaagcg aaaatgaatt caaataaaaa ggctctaagt
    14641 caaatcgtgt tgttttcatt aaatcactga aaggaaatgc ttctgcgttt ccttttttct
    14701 ttttgttgtg cgctgtatac cttgaggata ttcactggtg gcgtacactt aaatagtaat
    14761 atattgtgtt actttatgtc tgtggtaata gttatagttg cttaccaatg tatgttcatt
    14821 tttcgaaatg ttgacttcat aatccttggc ttaatgtttg aatttttttg gtgatagatt
    14881 ttttcagccg tataaaataa atcgtaattc tattatagag agttgtgatt ataaaaagat
    14941 agcaagaaaa aaaaagaaag tttctataca ttttaaatat tttattccta tttctattgc
    15001 taaaaaagta gaaattctat atttaccttt aactctcgct tctcttgcaa atgtagttag
    15061 cattttaatc gataataaac tcaggtagtt atattacctt gaaatgattg ataattttgt
    15121 cacttctatt ttttttgcaa taaatttttt attttagtga aaaataagaa act
//
LOCUS       DACEQH010000065        15009 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.066, whole genome shotgun sequence.
ACCESSION   DACEQH010000065 DACEQH010000000
VERSION     DACEQH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.066"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(273..1154)
                     /gene="galU"
                     /locus_tag="IW833_002218"
     CDS             complement(273..1154)
                     /gene="galU"
                     /locus_tag="IW833_002218"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1308314.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1197..2216)
                     /locus_tag="IW833_002219"
     CDS             complement(1197..2216)
                     /locus_tag="IW833_002219"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1308315.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(2849..3853)
                     /locus_tag="IW833_002220"
     CDS             complement(2849..3853)
                     /locus_tag="IW833_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1308316.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(3866..4915)
                     /locus_tag="IW833_002221"
     CDS             complement(3866..4915)
                     /locus_tag="IW833_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1308317.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(4932..5753)
                     /locus_tag="IW833_002222"
     CDS             complement(4932..5753)
                     /locus_tag="IW833_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1308318.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(5740..6519)
                     /locus_tag="IW833_002223"
     CDS             complement(5740..6519)
                     /locus_tag="IW833_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1308319.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(6536..7006)
                     /locus_tag="IW833_002224"
     CDS             complement(6536..7006)
                     /locus_tag="IW833_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1308320.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7018..7437)
                     /locus_tag="IW833_002225"
     CDS             complement(7018..7437)
                     /locus_tag="IW833_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ1308321.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(7518..10286)
                     /locus_tag="IW833_002226"
     CDS             complement(7518..10286)
                     /locus_tag="IW833_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1308322.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            10486..10710
                     /locus_tag="IW833_002227"
     CDS             10486..10710
                     /locus_tag="IW833_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308323.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            11489..13204
                     /locus_tag="IW833_002228"
     CDS             11489..13204
                     /locus_tag="IW833_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1308324.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            13207..14970
                     /locus_tag="IW833_002229"
     CDS             13207..14970
                     /locus_tag="IW833_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1308325.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         4362 a         3096 c         2585 g         4966 t
ORIGIN      
        1 aaaaaattcg cctttttatt ctactatgaa tcttctggct tggctttcat ttctcttatg
       61 taatcatttt cttttataca acttaaaaaa aaattaaacc attagcataa tattttctga
      121 atctattcac gaaagtcttt ttctatcttt cgttttccac gactcaactg atctagagtc
      181 aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct gccgctttaa taacaaatag
      241 cctttcattc tcagcaggcc acttatttcc agttaacttt ctgttcttag ctttttactc
      301 aactggataa tatatgcact taatgaagtc ccaatttccg aatgttgaag tccatattca
      361 atatttgtct tcatatatcc taacttatcg cctacgtcgt atctttttcc tttaaactct
      421 ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg catccgttag ctgtatctcg
      481 tttcctgctc ctggttcttg agtttctaat atctcaaata ttttaggagt cagtagataa
      541 cgtccgataa tggctaaatt acttggtgct tttcccattt caggtttttc tacaaaccct
      601 tttactctgc aacgaccctt ttcgctatct gttttaggat caataattcc gtatttagat
      661 gtttcatttt ctggtacacg catgacggca acagcggaag cttgtgtttc ttcataatcc
      721 atcatcaatt gttgagtcaa tgggatatca tctttcatca aatcatcccc cagcatgacg
      781 acaaacggtt catttcctat aaatgctttt gcttgaagca cggcatcccc cagtcctctt
      841 ggatgagatt gacggatgaa gtgaagattg acttgagtcg tttcttcaac taaagcgagt
      901 aattctgttt tttccttttc ccgcaaattg ttttccaatt cgatatttgc atcaaagtga
      961 tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat tcccgaatcc
     1021 aatgcttctt ccacaataaa ctggatcgtt ggtttatcca cgattggaag catttctttt
     1081 gccatggctt ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg aatgactgct
     1141 tttttgacct tcacttgctg ttcctcctaa acgacgatta actattgatc atctatttaa
     1201 cgaagagaaa attcattctc catttttccg tcacgtaaca tgatttcttt tgctgctttt
     1261 ttgatatctt ctccattata caaaacatta tagattgttt gagtaattgg catctcaaca
     1321 cctagctgtt gagctagctc tactgctgct tttgtcgttg ccacaccctc aacgaccatt
     1381 cccatgtttt ctagaacttc ttccagctta tgtccctgtc ctaacaagtt accggcacgc
     1441 caattgcgag aatgaacact tgtacaagta acgatcaagt cccccacacc actcaaaccg
     1501 ataaatgtca gaggattggc acccatcgcg acaccaagac ggctgatttc tgccaatcct
     1561 cgagtcatga ttgctgcttt tgcatcatct ccgaatccta atccatggat agcgccggca
     1621 ccaattgcaa tgatattttt cagtgctgca ccagtttcta cgccaatcac atcattattt
     1681 gtataaatcc tgaaataatc attcatgaac agtttttgta cataggctgc tgctttttga
     1741 tcaatacttg cagctgtaat tgttgtgata tcatgtactg ccacttcctc agcatggcta
     1801 ggaccagata atacaacaat tgcctgtctt ttttcttctg gaatttcttc agccaaaact
     1861 tctgagattc gtttatgcgt atcttgttct agtcctttac ttgcatgtat aatgattggc
     1921 ttagtattca ttttaccaat aagttcctgt gcgactgaac gaatcgcttt tgttggaaca
     1981 acaaataaaa tagcatctgc gttatccact gcttctgcca attctttata tccgataatc
     2041 gattctggga tcgtcaaatc aggtaaaaaa tgctgatttg tatgatactt attgatttca
     2101 tcaatctgtt caggtacatt tccccaaata cggacatcat gcccgttttc tgccaatgtt
     2161 tgtgctaaag cagtgcccca agatccagga ccaagaactg cgattttttg tttcatggat
     2221 attcctccaa ataaataatt gataatttcc attatagtat acatttctta aagttgggac
     2281 taatttccat tgtttttaag tgtttgttta cttcttttat cgtttttact tgaaataatc
     2341 aatgatatgt tacaatagtc tacattttat ttttacactc gttatgcttt gcttgttcct
     2401 aagaggaagc agttttgaca atgttactac cagcattact attaggaggt taggaaactt
     2461 ctcctagtga gtattgattt aaaatacata agagacagat tgaacatttc taaatctgtc
     2521 aggtcctgca acggacagat tgtctctgtt gacggttgac ataggaactt ttgttttagt
     2581 caacattctg gatacaaaac gctatgtaaa ctggcgcaac tcgttgtact tggactttta
     2641 ctacggatcc atgcaacaac taaatagtac tattgcataa caaaaaatgg ttggcaccca
     2701 catgtcccat taaacagatt ataagttgaa ctgtagataa tgcaaaaaat gtgttttata
     2761 acgtaattga tgccaatatt ttaaaaaaat aaagggagca gttatcgctg ctctctttat
     2821 tttttatatt ctttttatcc agtctatcct aataatcaaa ctgacgatag tagcggcgta
     2881 tctctaatgg atggcagttc aactgctcca catgagcatc gatgcgttgt gtcactacat
     2941 gcatcaataa gaaagacaaa acacgtccgc gatatttttc actgatacct tctactggat
     3001 agtcttttgt gtctatcaat gtataattgc tgcaaatttt tggcaaaagt ttagccacac
     3061 gttcagataa cactctctgt tcatcttcgc ctaaaaataa tgtgactggg gttgtttctt
     3121 ctacgatctc taacgtcccg tgaagaaact cggcagcatg gatagatttc gatggaagcc
     3181 agctttgttc ttcccagtaa cacatggcat atgaataaac agctccccat tgattccctg
     3241 ctccaataaa ataatgcata ctatcacgtc gatgtttttc tgcaaatgat aagccaaatg
     3301 catctgcttt tttctctgct tccactaacc ctgttggtaa ataacgttcg agctgttcat
     3361 agtattctga ataagcttcg aattctccat tcaaatacat caatcgatct gctgccataa
     3421 aaaatttgat ttgttctgtt ccaggagctg gataagtgac cacaaaatcg catagctgag
     3481 acaacttact gttcgcttta tcgataaatc caatcagtgt cgctcctact ttcttgattt
     3541 gttctactgc tttcactact tcttgagttg taccggtaac agaggacaaa ataacgatag
     3601 aatcttttgt taatcttttg tttccagtag tgtaatattc tgcagcatgc tgtacaaaga
     3661 caggcaagtt gcttttccca ttcatataag tgacagcttg catcgaggaa gcatacgttc
     3721 caccgattcc tagataatag acagcgtcaa atttttccgc ataaagttta tcgatgattt
     3781 cttctacttg cggacgtaaa gctagtgcgc cttttattgc tttgatttgt tcttcttcat
     3841 tgaatttcag catgatttcc acctcttata atttactttt caagactgta tcaaagtctt
     3901 caaaaatctt tttgttcaag tcaatacctt tagctactgc ggttttataa gcgaatagtt
     3961 gaaacggaac gacaagcgct aatacagaca gatattcatc acattctatt gctaatccaa
     4021 gtgttttctc gtttcttgct ttttttgtgg taatggtgag agtctctcca acgtaccgac
     4081 tcatatatct ttgcaacttt tgcgaacgtt ccttatttac actttcccct tcaataaaga
     4141 atagcaaatg cgaagcatct gcctccaggt atgggccgtg catataagct tctaactcaa
     4201 atccttgtga cggaacgcgg attgtttcag tcaatttcgt ctctgcttct ttagctgttc
     4261 cccagtttgg accatatcca atggtgacga aacgctgtgc taaacgaaat aaactttgat
     4321 atccatcaaa aaattcttcc gttttttgaa taatcgctgg taaatggttg attattttgc
     4381 gcaactgttg catgtagtct tgttctattt ttttgctgat tttattttta cttattccaa
     4441 ttcctatccc taagagtagt aactgtaaaa cagtcgcaga atagcctttt gtaacgaagc
     4501 ctactgattc taccccggca cctaaatcaa taatttgtgc agcttctaaa gcaagtggac
     4561 tttgcacgtc atttgtgata gcaacaacag gtaacccttg tttttgtatc atttttactg
     4621 cttcaacagt agaagcactt ttcccgcttt gtgacacagc aatcacagta tcgattgaag
     4681 gagaaagttt tccataattg aaaaaataaa aaggttcttg aatatcgaca gtaagatcac
     4741 cataagattc caatgctggc ttcgctgcta aacaagcgtt ataagatgaa cccgttgcta
     4801 agatcagtaa attcactgtt tttttcatat gttctcttgt cgaaaaatca ttctttttca
     4861 agatcgtact tagtacttcc ttttcttcat agatataatc ttgcatcgta aacattttct
     4921 ctgctccttt tttaaaaaat ccctatccac gaaccaaaaa ttccaaagaa agctagtccg
     4981 atcaagatcg ttaatggttt cacttctttc ttcaatagat aaaacactat gccaaatgct
     5041 ccaagacata agatatttgg aacgatatca tcaaaaatac tttgaacagt cacagcattt
     5101 tctcctgaac cgatttttaa aggaatattg atggtgatca tcgtggcagt cattgctcca
     5161 acgaccatca gtccaataat agacgcgcct aatgtcaaac tttccatcat accgtttgcc
     5221 tggatctttt tcaaaaaacc cgttcctaag cgatatcccc aacctgttgc tagatagcga
     5281 aacagaatgt gcggaatatt gaaaatcaaa atgaacaaga tagggcctaa aatatttcct
     5341 tgcatggcta aagaggtacc cacacctgtg gcaatcaatc gcaaagttcc ccaaaagaaa
     5401 gaatctccta accctgcaag aggtcccatc aaagatgttt tgatactatc gatcgcagtc
     5461 acatcaaatt caggatcatc cttattttct tcctccattg cagccgtgat tcctaaaatc
     5521 agtgtaacga tatgtggggt cgtgttaaaa aactctagat gccgtttcaa agctgctgcc
     5581 atttcttctt tcgactgata taactttttc aaaatcggaa tcattgcata aacataagcc
     5641 atattcatct gacgttcata attccaagaa aactccattt gaaaagaacg ccaaaatacg
     5701 tgatttagct cttttttcgt tattttgctg tttttgatct cagaattctt catcttcttc
     5761 tacctcccct gcagttgttt gaagtggtag ttgacggaca ttcataatat tgactaccac
     5821 aactgctaaa atcgcaccaa aaatagctac tccggtcaca ggaatctcta agtaagaagc
     5881 tatagcaaaa cctaagaaaa aatatggtgc aacttgtttg ttgatcagca aacgagcaag
     5941 catcgcaaaa ccaagagctg gaatcaaccc tgtagctact gacaacccct tttgaacgaa
     6001 gtcaggaatc atatttaata gatttccgat cgtattgctt cccaccatat aagaaaccat
     6061 taccacaaca ccaagcatca aggaaagacc gataccggca agcaagtgca tatgctcgac
     6121 tcctttataa tttccttctt ctgcataatt gtctgcttta tggttcataa ttggaatcaa
     6181 aagccctaag taaatatttt tcaatatcaa tgtcaacgta gcaatcggca aacctagtaa
     6241 aagcgcggtt tctgtaccgg ctccagctgt aatagcaaaa gccgtcccta aaatcccacc
     6301 ggtcacaaca tcaggtggaa tcgaagcacc tactgagaac gatccgataa atgctaactc
     6361 taaagttgcg cccataatga tcccagtttt tacatcacct aacacgattc ctgtaaacag
     6421 ccccgtcaca atcggacgag aaagcaacga tgtccctagt gcatattctg attgtgcgac
     6481 aaatgcaaca attcctaata agactgcttg tacaatcatt gaggttcttc ctttcttaca
     6541 aaacttctct tacatccact tttttatctg ctggtacttg acggatttct acttctattt
     6601 gtttgtcaat taatcgtttc aagcgttcct cctcttctgg taaaatattt accgcttttc
     6661 cgatgctgcg agtaccaggt tttgccttga tccctcctaa gttgatggtt ttgatttctg
     6721 gatacgcttc tgccagctta tatgcatcct cgatcgattc cactacgatg aataacttat
     6781 atttatctgt caccccgctt ttcaacgcgg caatcgagtc atcaattgat ttgatcacta
     6841 gtttgactcc ttgtggcttg gctaatttga tcgttgtctt tcgtatctca ttcgtaggta
     6901 catcatcatt agcaatcaaa atacagtctg cgcctattgt ttgtgtccat gaaaaagcta
     6961 cttgtccgtg taataaccga tggtcgactc ttgtcaatac aatcattttt cttcctccta
     7021 aaattcgtct tcttctattt tctgattgag ccgctcgtta caaaactgga tcattttttt
     7081 tgaatcagtt agcacctgct ggatcaaaac agttgtctct acttcagcgt ccaaccgaga
     7141 tactagctca atcaatagtg gcaaattcaa tccggcaacg agatagacat gatcccgatt
     7201 gatatattgt aagaattcgt tgttgacact tccgccaaaa agatccgtca gaacaatgat
     7261 ttctttatct ttgtacctca taaaaagctg ccttactgtt tccgctaaat caaaattctc
     7321 atcagtatag caatcgaata cagtaataga atgatttttc ccatagatca gctctaatga
     7381 atctaatatc cctgaagcaa attttccatg ggaagctaag ataatttctc tatccacttt
     7441 ttcatctcct cttttcactt acacatacta aaaaagcaag ttgcatgcca aaattagtgg
     7501 caacaacttg cttatgttca aaattcttct tctatcgaga gaaactctgt atcttcactc
     7561 aaaatatcat agatataggc aatctcagaa ttggggatca tgacactata atgctcttca
     7621 atgacactaa acgcagattt aatagcagat aacccttctt tttgacactg cttccatttc
     7681 tctaatcctt gataggtttc gattggcatt tgtcggatca atcgttcaat gagacagctc
     7741 acatggacat acaacgctaa ttttttggga ttgctgatgc ttcgattcaa ctgcacctct
     7801 aattctctca taaaatgatc gatttctccc atcaccttct ctgtatctag aatagtcacg
     7861 gaatccagta ctttttccaa tgaaaaatta cgaatgatgc gctgattgaa tatttccttc
     7921 tctgaagcat tcatagtagt gtccaaccat tcagctagta cagacatctt ttcccccgag
     7981 atgaggtctt ctaatgaaag atagtcgata ttggtgatat caggatctgt ggtgccaatc
     8041 attcctacga cttcgtatat ggaaaataat gggtcactgt ttttcttatc ttttaattgc
     8101 tgatattcat atgggatgat tttcaactga caagccgtag gtaagctttt ttctagcagt
     8161 tcacttaagt ggaccgccgt accgattcca gtgaagcaag tagtaatgag tgctttgggt
     8221 ttgttttcct ctggatagag cagtcgccat tctggctgga tctgtcgaac agtctcttct
     8281 gctatctctt ctaatgatac atttttttgc aggcattccc ctactgtgat tgctaaaggt
     8341 gtcgtcacat tattcaaaat caaaagcggt acggataact gttttttaaa aaactgatgg
     8401 atttctttca gagatcccat gtctactaat atgatcaatc catttgaaac atcgctgttt
     8461 tcgcaataat caaggatttc tgaagcaatc tgctgtggtg tgacatctag tggcatatca
     8521 aatgattcaa atagatcctc ttgtaataaa cgatttacta catttgccat actgctagca
     8581 gttgcataac cgtgagcagc aatcatcgct tttggcagtc gtttttcttt tgaccatttc
     8641 gcttttttta gatagatcgt cagtaaaatc cgatccatac ttgtgtcttc aatatctaat
     8701 ttgatcttac ttaattccag aattcgttgg acatagtgat aactagatgg ataagactga
     8761 gaaacttgtt gttctaattc tttgattagt tggataatat catgttcttc cggtaaccat
     8821 ttgatagtgc ttcgctgata gagatagtaa ctcaaggcat agacactgtt tccatcgaat
     8881 ttgatttgat aggcagtttc catttgtcga aaggtctctc ggatagattg tgtccaataa
     8941 accagcagct cgtgtttttc ttgtctactg gtctcaaaca gcaaaaaatc aaataattga
     9001 atcacttctt gctttaattt tccttcgcaa gaatatagca gacagttatt tttcttgaaa
     9061 tcaattagga tttgctcaaa acttttaacg attcgctgct gttcgggctg ttgcttctcc
     9121 attaattgct ctaaggtctg gtcttgcgta atatagattc cttgctgtgc tgtattttta
     9181 gtaataggct gcgcaagtag ttctttttgc aaatgatgga tcgtgacatg gatttcaggc
     9241 tgttcacgct gttcagcata tgcttttgct gctgaaactt tgacgatatt tttcagctct
     9301 cctatgtttc cggataattt ctgacttgtt aaaagtgaca aagctggtcc actgactgac
     9361 aaagggctcc ccatttttct ttgttcttcg agaaaaaaag agtaaatcag ttctagccgt
     9421 tcattacgac tacgctgatt aagtgcaggt agttcgattc ggataggtat tcttcgaatg
     9481 aaagttgtta agaaatgact agtaatctct tcagtcgtag caaaaaagag acgtgttttg
     9541 atccggcgtg acaaattagg ttctcccata cgataaatca caccttgatc aagataggtg
     9601 aacagttttt cctgaccttc tggtgttaag cgatgtacct cgtctaaaaa caagatccca
     9661 ccatctgctg cttcaaaagc ccctttttta ttttcttctg caccagtgta cgctccctta
     9721 acatgtccaa acaaattgct agtcaaaagt tcaggattat ctgcatattg tgcacaatta
     9781 aaagtgatga atggtgcaga atcttctagc agatcatgaa gcagacaata ctgatagacc
     9841 aaattaacta aataactttt acctgtgcca ctttcgcctg taatcaaaag tggtaatccg
     9901 ccatctggat agtaaagagc tgttttgatt tgttcgatac tttctttcaa actggcgtcg
     9961 tatccgatca taaaagagaa taagtctttt ggttgcgcga aaaaaggctg ttcctctttg
    10021 agatcatcga tagaaaaata aaaattcgtg cgtaacttaa aaaattgttt ttcaaaagct
    10081 tctttgtgga aataaatcac tggtcgagaa ttgattttga ctagtagttg ctcatcattt
    10141 aactggttca aataatgact gactgtgttt ctttttaccc caaaatgatc agctaacctt
    10201 tgtgctgtaa agacctcgtt catatgttct aaatcgaaaa aggctgtctg gttctctaag
    10261 taacataaaa tttccttctt caacaactta ttcacccctc ctccatcata gaaaacggat
    10321 tcattttgtc aataaaatct ttaataaaga attgaaaaat atatcttatt atagacagca
    10381 gcctacttat cccttataat ttttattcta aaaaacgtac agttccttcc agtttttata
    10441 ctggaatttc aacatatgaa agaataagga ggctatgact attcaatgaa atcggataaa
    10501 tataaaaaca ggaaacatac ttcctggaaa acttttcgag aaatcatcgg atcttatctc
    10561 gctgtagttg tatttttgcc tttgctttat atggctatga cagaacaaac tcgttttatt
    10621 ggcgtagata cgcaatatat agccgaaaca ggatttttat tcatctttat cttgattttt
    10681 gcgttctata aatccttcca tacaaaataa gattcacaga tttcaacttt cctgtctgtt
    10741 tttagctatt acaaatcctt atatgtggta taacaacaat ttattggcat aatgaggaaa
    10801 aacgagttat gatgctttca gagagtaaag aggtactgtt gtcaccatcc tttttattga
    10861 ttacgtacac cactcttagt tttttcccat tgattacgaa aacaaggtag aaacattgat
    10921 gttcctacct tgttttcttt ttttattttc agatcaaaat ctcaagtagc ttttgacttg
    10981 atgtcgggag gttgtcttcg ttccatcaaa cctgatagaa tctgcgccaa accatagttt
    11041 tctccactat tatttattcc tctaagatag tacccacaat aaacgaataa atatttttct
    11101 cattttcata ctccttcttt gaaaatcaat tagatttctc tcaacccact tttttacaaa
    11161 aattacgtag caaaaaagat tgcaagaaca aattaaaaaa gaagtaaata gagtttcatc
    11221 tataaacatt tacttgatat attttttatg cagaattcat ttatagaaaa attaagtaaa
    11281 atagacgttc ctatgaatga agagactggc atgttttctc attttctttt ttttaaaaaa
    11341 aatagtctat tcaatttcta aagcttcttc ataataaaac tttcatttat gaaaaggctt
    11401 gttctctttt cattctaaat ttcctaagct acaatgtggt agtacttgtt ttttcaattg
    11461 tggacaaata aataatagga ggaatcatat gattcagtta ttacgttatg caaaagacta
    11521 tcgtaagcaa atcattttgg gcccgttctt caaatttctt gaagctgtct tcgaacttgt
    11581 cctgccttta atgatggctt ccttgatcga taatggacta aaaatgaatg accgaggaaa
    11641 aatcattgaa atgggcttat ggatggtcgc tatgtctgtg attggtctga tttgtgctat
    11701 catttgccaa tactatgctt cagtagcttc acaaggattt ggtacagagt tacgtaatca
    11761 gctaatcaaa aaaatcaata ctttctctca caacgagttg aatcattttg gaacagatac
    11821 cttgatcacc cgaatgacaa acgacatcaa tcagcttcag ctagctttag caatggtcat
    11881 tcgtttattg atcagagcac cgtttttaag tatcgggtca gtggtcatgg cttttgtgat
    11941 cgactgggaa gtaggtctgt ttttcttagc attattgcct attttctcaa tcattttatt
    12001 ttttatcatc aaaaaaaccg ttcctctcta ccaaaaagtg caagaaaaac tagatcagct
    12061 caatgaacac gttagtcaga atcttagcgg tgttcgtgtc atccgcgcat ttgcgcagac
    12121 aaaaaaagaa acagagaagt tcgataaaag cacaaatgat ctagcgaaca attatttacg
    12181 tgtttccaat atctctgccc tattatcacc tgctacaacc ttgatcatga atgtcgggat
    12241 catctgtttg ctgacagttg gcgggatcaa agtaaatatt ggtagcttgc aacaaggaca
    12301 agttcttgct ttgatcaatt atatgaatca aatgcttttg gcactgatcg ttgtttcaaa
    12361 cctcgtcgtg atcttcacac gtgccgaagc ttcaggaaat cgggtgaaag aggtgctaga
    12421 cacagaaaat tcgattcttg aagctgaggt cagtactact cccgattttt cttctgaagc
    12481 aattatcgct tttgaccatg ttgacttccg ttatactcct gattctggtc tttctctcca
    12541 aaatattaca tttcagctga agcgaaatac agtccttgga atcattggtc caaccggaag
    12601 cgggaaaacg actttgacac aattgattcc gcgtttctat gatgtcagtt ccggtacagt
    12661 tataatggac ggaatggatg ttcgttcttg gcagctggat catctccgtc agcaaattgc
    12721 tcttgttccc caaacttctg tactatttac cgggactatt cgtgaaaacc ttcaatgggg
    12781 taagaaagac gccacagaaa aagaatgttg ggaagctctg aaaatcgctc aggcagaaga
    12841 atttgtccgt cagcttccaa atggtttaga tacaaaagtc atggaaaatg ggaaaaattt
    12901 ctctggcgga caaaaacagc gactgacgat tgcaagagct ttgattgcca agccagcatt
    12961 actgatttta gatgactctt taagtgcgtt ggattatcaa acagacttgg atttgcgtaa
    13021 ggcgctacag acttatcttg gcagtacagt gattattatc tcccaacgtg tccgctctat
    13081 tcaagaagca catcatatct tagtgatgga tcaaggtaag attgctgctc agggtaccca
    13141 tgaagaatta cttgctcaat cagcagaata tcaggaaatc gtcgcatcac aggaggaaga
    13201 atagttatga aagtaaaaaa attatccttg cagacgctcc gccgattcat tccttacctt
    13261 actgcctatg caaaagacat ttggctagct gtgatactag gaatcatcaa tgggattgca
    13321 acagttgtga tgacgttaca aattggtcaa agtatcgatc aaatgattgg agaaggacag
    13381 gtccgcttca atcgactgtt ccatttactt attctattcg gtggtatcgt tttaatcaac
    13441 gtagtcagtc aatggctcat ccaagtgtta agcaatcggt tagcttactt atccgttgca
    13501 aaattaagaa aagatgcatt tgctcatctg aatcgattgc cacttcgtta ttatgatcaa
    13561 aactcccacg gaaatatcgt cagtcgattt acaaatgata tggacaatat ttccattgca
    13621 atttctgcgg ccttcaatca attattttct ggagtggcaa tcatcattct ttcgtttatt
    13681 tttatgatgc gtctaagtcc acttctgaca ttggtcgttc tttgttctac accaattatt
    13741 ttccttgtga gttggatcgt tgctcgtgct tcacaaacaa atttcgatag acagcaaagg
    13801 attattgggg atatctcggg ctttgtgacc gaacgaatcg gtaaccagaa aatcgtgaaa
    13861 gcttttcagc aagaagaagc tagtcaacag caatttgaac tgctaaatgc tgatctatat
    13921 gaaaaaggac aacgggcaca attttcttct tccttgacaa acccgtcttc tcgttttgta
    13981 gatcaccttg cttatttatc gattggttta gttggcggcc tattggcatt gaagagtggt
    14041 tcgtcaatca ctgttggtgt gatttccagc tttacgatct actcaagtca gttttctaaa
    14101 ccatttatcg aactttctgg aatcacgaca caactgcaaa ccgctttttc tggtctagaa
    14161 cgagcatttg atttgatcga tcagccagaa gaaaagcctg atttgccaga tgcctatgcc
    14221 ttggaaccca aaaatatcaa aggaaaagtc tcttttgatc atgtttcttt ttcttatgac
    14281 cccaagcagc gtttgatcga agatttcaat tttactgctt atccaggtca aaccattgct
    14341 atagtaggaa aaacaggggc cggaaaatcc acacttgtta atttgctgat gcgtttttat
    14401 gacacaacaa aaggaacaat tacagtcgat gaccatgaca tccgctctat ccaacgtgat
    14461 acattacgta aaagttttgg aatggtgtta caagatactt ggctatttga cagtacattg
    14521 cgagaaaatc ttcgttttgg gaatccggaa gcaagtgacg cagaaatcaa tgatgcccta
    14581 aaagcctctt atatgtatga atttgtagaa cgtttgccgg aaaaattaga tactaagatt
    14641 ggcggtcaag ggttaaagat atcagatgga caacgccaac tgttgacgat tgctcgaacg
    14701 atgatcagta atccccctat gttgatctta gatgaagcta ccagctcagt cgatacattg
    14761 acagagaaaa aaatccaaca agccttctta gcaatgatgg aaggaaaaac cagttttgtg
    14821 attgcccatc gcctgtccac aatcaaaaat gcggaccaga tattagtgat ggatcaggga
    14881 gctattgtcg aaatgggcac ccatgatgag ctgcttcaaa aagatggtta ttaccgacgg
    14941 ctctatcaag cacaattcac agcacaataa aaacaagcag tcttctggga tgggaagact
    15001 gcttgtttt
//
LOCUS       DACEQH010000066        14046 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.067, whole genome shotgun sequence.
ACCESSION   DACEQH010000066 DACEQH010000000
VERSION     DACEQH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.067"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1412
                     /locus_tag="IW833_002230"
     CDS             <1..1412
                     /locus_tag="IW833_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1308326.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..2889
                     /locus_tag="IW833_002231"
     CDS             1546..2889
                     /locus_tag="IW833_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1308327.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            3001..4404
                     /locus_tag="IW833_002232"
     CDS             3001..4404
                     /locus_tag="IW833_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1308328.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            4753..5385
                     /locus_tag="IW833_002233"
     CDS             4753..5385
                     /locus_tag="IW833_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ1308329.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            5973..7412
                     /locus_tag="IW833_002234"
     CDS             5973..7412
                     /locus_tag="IW833_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1308330.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7707..7925
                     /locus_tag="IW833_002235"
                     /pseudo
     CDS             7707..7925
                     /locus_tag="IW833_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8174..8389
                     /locus_tag="IW833_002236"
     CDS             8174..8389
                     /locus_tag="IW833_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308331.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8606..8862)
                     /locus_tag="IW833_002237"
                     /pseudo
     CDS             complement(8606..8862)
                     /locus_tag="IW833_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(9049..9960)
                     /locus_tag="IW833_002238"
     CDS             complement(9049..9960)
                     /locus_tag="IW833_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1308332.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(9979..10983)
                     /locus_tag="IW833_002239"
     CDS             complement(9979..10983)
                     /locus_tag="IW833_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1308333.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(10980..13103)
                     /locus_tag="IW833_002240"
     CDS             complement(10980..13103)
                     /locus_tag="IW833_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1308334.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(13108..14028)
                     /locus_tag="IW833_002241"
     CDS             complement(13108..14028)
                     /locus_tag="IW833_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1308335.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         4588 a         2495 c         2643 g         4320 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tgggaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tattaagtga gtctatagat tatatagaaa tagaagattt cattagacct
     2641 gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag
     2701 agaatagtag gtgtacaact ttgtagtcaa gaagatttat ctggactatt atcaatgttt
     2761 tctcttgcta tagaagaaca aattacatta gacaaattga aaatgttaga ttatttattt
     2821 catcctagtt ttagccaacc ctataatttt attttaaagg cagtaacagg attaaacatt
     2881 gaaacataat cgttactaaa tgaaatcaag aaatatactg tattactcaa ttcattgaaa
     2941 atatttatca taatttttaa tagaaaacac tattaaagag aaaaaagaga ggtaaatcaa
     3001 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
     3061 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
     3121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
     3181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
     3241 agtagtacga cttcaaatgc cgagtcttca acaacttcgt caagtgaaag tagtacgatt
     3301 tcaagtaccg aatcttcaac aacttcaagt agtacaagtg aaataacttc ttcgaaagtg
     3361 acaaaaccaa accatataga atcttcaaaa aaggaagcgc caaaaacgga aggacaaaca
     3421 aaaacttttt cgacaccaca gcaaatagaa tcaacagaat ctttccaatc acaaccaata
     3481 ctatctggta atgaagaaat tcattttgac aaaaatgaat ctgtagaaaa ttttattatg
     3541 aaaattggtg agtctgcgag aaaaattggt cagaaatatg atttatatgc ttctgttatg
     3601 attgcacaag cgatcttaga atctgccagt ggtcaaagcc agttagctca ggcaccgaat
     3661 tacaatttat ttgggataaa aggaacttat aatggaaact ttgtcattat ggtaacaaac
     3721 gaagatttgg gtaatggaac gctttatacg acccaatcaa aatttagagt gtatgaaaac
     3781 tatgaggaaa gctttgaaga ttatgccaaa ttattaacta agggaattag tggaaataaa
     3841 gatttttatg caggagcatt aaaagctaat agtaaaacat atcgagaagc aactaaattc
     3901 cttacaggac ggtatgcaac agatacacaa tactatttga agctaaatga attaattaaa
     3961 acctacgatt taaccaacta tgataaagaa gttaaaggtc cacaaatgag taagactgga
     4021 taccaagtgc ctattcataa ttattcaata accagtttgt ttggaaatcg tggaaatgaa
     4081 tttcaccgag gacttgatat ggcagctaca cagggtgagc cgattcatgc agttaaagct
     4141 ggtaaggttg ttaaagcaga gtttcactac tcttggggaa attacgtggt gatacaacac
     4201 gaagatggat caaccgcgtt atatgctcac cagcaacaat acctagtaaa aactggagat
     4261 aatgttttgc aaggacaaat aattggctat gtaggaagta ctgggaatag tactggccca
     4321 caccttcatt tggaaatttg caaagatagt agtctttctc agagcatgtt agttgatccg
     4381 aaaaccttat tattcggaga ctaaaaaagg taatcaatct gacaaagtaa caagaaaatg
     4441 gttggattct tagacctttg atcaggatat tgagactgga attgatatct cgattacgat
     4501 gggtttgaca aataaaacaa gtccattatc gaatatcgag tgtattattg ccgtctttat
     4561 gctcatattt tgagcaggtt tcactagatg aaaacaattt atctatcttg aggatttcgt
     4621 gtacgtatcg ttcagtcttg tattgtaaat tagtcataaa atcagactaa gaaaaattgg
     4681 aaatattttt ttctgattta ttaaaagtta attgaaatta tatggtactt ctttcttggg
     4741 agggtaggtg cagtgaagga aagatatggt caccttactg ttgttgagga gattactcat
     4801 aatggatata aaaaatatcg ttgcctatgt gactgtggga atgaaacgat tgtatatcga
     4861 tcaaacttac tatcaggtag aaccaaaagt tgtggatgtg gaaattataa aaataggcat
     4921 aaatataaaa atttgacaaa taaaactttt ggtaagttag ttgcaatcaa gccaactaaa
     4981 ctgcgaagtg aaggaactgt tgtttgggaa tgtcaatgtc tgtgtgggag aaaaaagtta
     5041 gtatcggcaa agcgacttct aaaaggtgat gtaaaaagct gtggatgttt gaaatcaaaa
     5101 aagcatcaca ttgaaaataa aaaattcgga ttacttactg caaagtatcc tttggacaat
     5161 aactgttcat ggaaaacaaa atgggtttgt cattgtgagt gtggcagtat gtgtgagata
     5221 agttatagta acttattttt tggacatact caaagttgtg gatgtttaaa aaggcaagaa
     5281 acaaggacat gtgttgaagg gacagtaata gagtgtctaa cgtcaaaatt accgaaaaat
     5341 aatacaagtg gtgttaaggg agtttatcgt tctaaagaga aatagattgc gtatataact
     5401 ttaaaaaaaa agaggtacta tttaggatcg tatgattcta ttaagagtgc agctcaagct
     5461 agaaaagatg ctgaaaataa attgtttcaa cctttgattg acaaatatcg aggtaatcat
     5521 taattattgt ggataataat taacagtgat atataaacat ctataatata ttattcatac
     5581 tattgatatt tagtataatc ctatggaatt gaatatcata gcaatgtatt tgaaaagcaa
     5641 attggaaaac aaataggtgc aacaccataa atatgaaaaa attgggaaaa agtagaatgg
     5701 aaatttgctt acgtctcacg agatagctca cactattaat ctgaagaaca atgaagtcga
     5761 cttttagaaa ttgctagaag aaagtaaagc gaattatcac tagttactat agagtaagtt
     5821 caaataaagc atctccaact tacatggtta catgaggata tcaaatcgaa attactcaag
     5881 cttcctatgg ctagagtcgc gagaagttgt tgggcagtag acataggtta tattgttctt
     5941 agctacacga tcattagaaa ggaaatccta ttatgtatag ttatcaagaa gatgcaaaag
     6001 tccaaaaaaa tcgctggtgg attttgattg ctatatctat gtttaccttc atgtccacat
     6061 tggatggcag tattgtcaat attgcactac ctaccatctc agatgatatg ggcataccaa
     6121 tgaatcaagc ggaatgggta gtgtccgttt atctgatgat tgtctgttct tgtttgttgc
     6181 tgtttggtaa aattggcgat agccgtggga aaatcaaagt atttcggatt ggtacgctga
     6241 tctttgtctt gggatcgctg ttgtgtgggt tcaatcattc gctaggtatt ttattgttcg
     6301 cacgggtggt acaagccatt ggtgctagta tgactatggc tacaaatgcc ggaatcatca
     6361 cggaagtctt tcccatacat gaacgtggtc gggcattggg agcaataggt gcgtttgttt
     6421 cattaggctc gattgctgga ccaggtcttg gcggcttgat tttgtcccaa ttttcttggg
     6481 cgtacatttt ttggatcaat gtaccggtgg gaatcgtaac gatgttgatt gctgaaaaat
     6541 tcttaccaaa agatatcacc atgagcggcc atacaatcga tggagggggc tttgctgcca
     6601 ttgcggctac gattatcagc ttttttggag caatcttttt ggggcaagaa agtggctttt
     6661 tccatccagt cccccttgta ctgttcgtaa tttcagtgat tgcctttgtc agctttttaa
     6721 aagttgaaaa gaaagcagcc tctccattga tttctttcgg tatttttaag aacaagattt
     6781 ttaccatgag cttgattaca gccttgttga ttttttcttc caacttcttt gtcaatgtag
     6841 tgattccttt ttatctgcaa aatgctcgcg ggttgagtcc tagctatgct gggctgttga
     6901 tgatggtctt tcctttgatg atggtcgtcg gggcgccttt aagcggctac ctgaccgacc
     6961 gggttggccc ggagattttg gtattagtag gcttgggctt attgtcggta acccaggtca
     7021 tgtatatgtt tttgtcagtg gactctcctt tgtggctata cattgtcgct actgccatca
     7081 tgggtgccgg caatgccttg ttccagtccc ccaataatac gatggttatg agctcggtca
     7141 ctatcgaaaa cttgggactg gcaggctcga tgaattcctt tgcccgaaat ttggggatgg
     7201 tcagcgggat ttcgctttct acgacggtct tatatcaagc gatgtctgct aagatgggcc
     7261 agcaagtaac taactatatc gcaaatcggc cggatgtctt tttatatgga atgaagatta
     7321 cctttttagg cagctttgtt ttgtgttttg ttgccttttt cctgactatc tggcgtatca
     7381 aaaaacaagg aggtattcag aaaaatgtct gaaaaaacca ctgttgaaga atttttggag
     7441 gcctgcgtat gggtgagtgg gatttccatg gatgatggaa gatgaagtga acttctaaaa
     7501 gccgaaaaac tcaagtatta tcttcaaaat actagtgctc aatgagatgc ttgatttgat
     7561 cacaagaaga ctgcgaaagc cgctggcaac cgcaagctag gtatcccacc gatggactac
     7621 gttttagagg ctactggtta tgaatacgga gccaacgctc tcatctgcat aagaatacat
     7681 cacccagatt atgtaaagat tttcgatact tctgtcaaaa aatcattaag atagataaat
     7741 gatttccgtc tagtcaaatt ggttcagaat gtagacacac agatggtaag aagtcactca
     7801 caattcgaga atggacttgt cctatttgcc gtatacatca taatcgaaac gtcaacgata
     7861 gtatcaatat tttgaccaag ggctatgaat acaagcattg ccttagaaaa catcaagaac
     7921 cttaggaaca acggggatag cttggttaat aagagatacc gctgtttagg cttgcaagta
     7981 tactctttta tcaagaattc cgtggcttta gtcatgagag gcttaatact atgaaaattt
     8041 tctttagcat ttttgattta ggttcctttt ctaattataa taagatgacc actacagtaa
     8101 cctactgaaa tatataaaca gccactgtag taccattttc aaagtagtct atgagaggag
     8161 aatcttcatt cttatgaacg ctggaattgc ggatatgaat ttaataaaaa aaacccttaa
     8221 tgattttact tccaattcaa tatcaaaagg tactggtatc aatttaagca caataaaaaa
     8281 gctgaaaagt ggtgaaagat cagtggaaaa gctgaatctt ttagatgcta taaaaataac
     8341 ggaatttgct atgaaaaatg gaaaagctga gattgaaata tggagataat tatcgtctag
     8401 tgaattactt gccacttagc tgaatttaac gatccttggt gcttgaaggg ggagattctt
     8461 gggaatagag catacttgca agcctatgtc tttactaaca acgatatctc ttattaacca
     8521 agctatcccc gtagttctaa ggcttttact tttatctaag caataatcgt attcaaagga
     8581 aatttttgac ccttagaaag tctcatttta tcagacgtga gcttaggcat tgctgaattt
     8641 tgttaattcg gttttaggtt gtgctttgag cataatatga aagtattctt tatcacgatt
     8701 tcattcatct aaggtaatgt gatgtgattc tgaaattctt tcaaaggcag tttttgcata
     8761 agctgacatt acatcatcaa tgacatgttt acaatgtttc actactaaaa taagggggta
     8821 accaagaaga aaaactgaat gattatttgt atctaattcc atggtatatc aaccatttcc
     8881 tatatagact gattatgaca tggattaaaa taaaaaggca ctatgtctta attgtcggtt
     8941 ttcgagttac cgaatggcat tcatcaccca cctatagagg atgggcgact tctgcctgaa
     9001 ataagttaca ctacttataa cttctaataa aattagattg agtcattatt atttaacatc
     9061 aacgattttc cagtttccat ttttttgtaa tactaatttg tactgaaagt agctagtggt
     9121 ttttgataca ttatcaaggt attttatctt tgtactcact agaaaatttt ttccgttttg
     9181 tagataaact ggattctcta actccgaaaa ctttaagtca atattgattg gtcgcatcgc
     9241 accattttca acataatact ccaattcttt ttcagaagct gatggataaa gtttaaaaaa
     9301 tgtttctaaa aaatcggtga tttcttccgt tgttttggca tcaatttctg aatcgttttc
     9361 tacttgtttt gcttcatatt ttgccttgtc tggctttcca atcattgttg gcaaactagt
     9421 tactaaatga ttcctgtttt catcttcaaa gattgtcact cgatatgcag aagaaattga
     9481 ttgcgtcttt tttccctctg taatatgttg ctccactaaa aatgaaacga caaattcttc
     9541 cgagtgtttt tcaacatcta gaatttttac agattgaact tcggaactcg tgggaatatc
     9601 tgctcgaacc atatcttgga ttaaagcgag accgttttct gtaaggtact gttcgagatt
     9661 actcattctc ttttcaattg cttctttgtt atttttccat gaaaaatatt ctttcgcaaa
     9721 atcctctgta aaactttcaa cgccagaagt attcactacc ttctctttta taaccacttt
     9781 ttcatgagtt gtatgttgat caatggcggt gaaattttta taaacgccaa aagcaagact
     9841 acaagacaat aaaagccaaa gggcaagcac catttttcga tgcttaccca cggatacaac
     9901 tcgttctttt ttcttttttg ggctcttttc cttttgattc cgttcaattt ttatcttcat
     9961 tctttttcct cttttctctt atttgtatcg gccagctccg ataagatgct gccgccaata
    10021 gttttccgtt agattcgtcc accccactgg atctcctgca tgaaacatgc gattctcccc
    10081 tgcgtaaatg cccacatggg tcacataagt accggcatca taggttgaat gaaagaaaac
    10141 taaatcacct ggtttggcat cttttagatc aatatgagtc atggcatcat attgatcctg
    10201 agccacccga ggaagtttca ggtcaacttt ggcataacac cattgggtca agccactaca
    10261 atcaaacccc gtttctggac ttgagccacc aaacacatag gccgttcctt catacttaaa
    10321 tgcctcatca aaaatttctt ggactatttt gtcatcaaac tgagttgtag ttaggtattg
    10381 cttcactaat aaacagtaaa acatattgcc ataattatac cgccacccac cattttcctt
    10441 aatcgcgatt gggttggggt aatcgacttt ttgaccacct gatttttcct ttgaaaaaga
    10501 ttcagctaaa gcaaaactat gcttcttccc gtttttcccg acatgatcta gatacccgcc
    10561 accatagtta taggcctgaa taatactatc ttgatcacac cctagttctt tggaacgctt
    10621 aaccaaagac gcaaaataga gacagccttg tttgatggat gcctcggtac ttaatgaatt
    10681 aggtggtaat ccggctgact cagaactttg cataacgtct tccattgtgc caccagattc
    10741 cacttgaata atcgccaaaa gaattggaat atactctgtt atatccgatt ctttggcata
    10801 cttttccacc attgcacggt gtttcaatac ttcttctgag acactagttc cgccaggaga
    10861 agtgccagaa ctaccatttc cctcactgtc ttcaccaaac aacaaaccca cacacatcaa
    10921 aatacccata aaagagatgg tcagaaaagt taatgaaata acacccactt tcttcaagtt
    10981 cattttttct cacctgacct tgattttgga cgatggttca ttcttttttg tactttttga
    11041 ttggtctgct ttggcagagt ggacctctgt ttcacttgtt ggaaagaatt tttcttactt
    11101 gctgattggc ttttcccttt tgaaatgatt tcttgactag ttagttttcg tttgatttcg
    11161 ggattcagat ttgctctcac aatccgcttt ggtgcttctt ttttcagcat catatctttc
    11221 tgaggttgag ctgctttatc tattggaata ttcgcccttc tttttccctg acgatttgtt
    11281 gcttcactca gtatcttgcg tttttcgtcc attttcgcat ttcttcgttg tcgttgtttt
    11341 tctcgcaaat ccactcgatc cgctttttct tggaacagtc cgcgcttgaa ttcttctggt
    11401 gcttttttcg acttttcgat tcctttatgg agattgtatt tcaaattcgt tggcgtattc
    11461 cttacctgat cctttacttg tttagtctta tctttcacca aagcttgggt atccaaaact
    11521 ttgccgattt ttctcccagc caaattcatt cgggattgtt tcttattatt tgaaactgga
    11581 ttctccttag ttaagtcaga tagtatttcg tgatcgttcg gtaatcgtga attctttcgt
    11641 agagaagaac cggatggctt aaatttggat tttgattgtt cctttgccaa cttatttccg
    11701 actgctgcac caccaccaaa agccaaagct cgtcccacat ttcgattgag ttgccgcaat
    11761 tttcgattca ttaacatttg cggttttcgc atgacgcgtc ttcccattga ctggctatca
    11821 ttactctgga ggttgaacat actcataatg tcgcccaatt tgaaatagat accggcaaag
    11881 gtcacaattt gcagaaaagc aaccatgaaa aatggatagg ttgccgaaat attgaaaaac
    11941 atcgtggaga tgctaaatgc agtcgtaatc accaaagtaa cacccgctcg catcataatc
    12001 gtattgaata aacgaataat cgccttcttt cctaaacttt cataagtcgg caacatggac
    12061 aataagaaac taattggcag aaacatcgca aagataatga agagaatttg cgagaacaac
    12121 ataatccctg ttagaagaaa aacaaagaag gaaatgccaa tattgaacaa taccaaaaag
    12181 accacggtcc ctaaacggtt catggtcttg gtaatactga gattcgcatt gtcattgtct
    12241 tcaatctctg ccttgaccgc ttcttcccga tctttgcctt tgttttcatc tggactcaca
    12301 gatagaatct tgttgacccg gtcttcacca atttcttcta tgttggaatc atcaaattgc
    12361 agtagtaacc aaggttgttg gacttgaatc gaaaacaaac tatcccgaat caaatccaca
    12421 ctatcttttc cttggctttc tgaattgggt acaacgatct cggttccaag ggtaagggcc
    12481 gcttcactaa catctgagct aaagtcattg atttttgtga tataggtagg cgcataagca
    12541 atgaacgaac ccgatagtag aaaaatcatc acaaaattaa tcacggctcg aactgctttc
    12601 gtggtttctc gttttaataa tccggtatac gccacataca cccccagagc taaaatcatc
    12661 aataatagaa acccaaaata aaatccggag gtctggagtc cattttcagt aataccagcc
    12721 aatgtttgga tattctttcc aatactttca gccgtatccg aaatgaaatc taatttgtaa
    12781 gcctcttgaa ccacataacc cgtggcgttc gacaaataca aactcaccgt ccaaatgaaa
    12841 ttagtaatgg cataaagacc atacatcacg cttttcccta agccatcacc ccaattccaa
    12901 ggcaaccaat cccaagagct atccacaaag aaatctagtt gatagttgtt cagtgaatat
    12961 ttggaatata gatttcctgc ttcaactgta tcatcaacta gccctgtcgc ctcggctacc
    13021 gtaccaacta aactcaatag aactatcaga gtgagtatgc taataaaaac gattcccaca
    13081 atccgaaaaa gtttcttttt catcccctca ctccattctc tcttgaacag gtggacgggt
    13141 atcaaaggca tggaagagtt cttcaaagac tggatgtacc tgaaccacgc ctacccgacc
    13201 atacaaatcc tgcatcaagc attggccatt ttccaaatct cgtaggcgtt tttgattgct
    13261 ttcatcttct cgatccaagc caaagaattc caaggttttc ttgatttcat gaatatcggt
    13321 actgcgaaac gcaaacttca acccgatatt attcttcaag cgttcatcca gtaaatcatc
    13381 actattttgc gtgacaaagt aaaccccggc attcatagcc cgcccagcac gaacgagctt
    13441 attcgataat gttttccctt gcgctacttg aagaaaactc cacgcctcat ctaagtctac
    13501 aattttgaag atttctcgat tactatggat aaagtccaag gcaaaagtgg aaatgacgat
    13561 tagcatggaa acactaagca attccatggt ggtatattct tcaaaagtgg tttctgcatc
    13621 tggtaaaacc aaatccgcta cttgaatgat atttaactgc ctatctaaac taatcgactg
    13681 ggtaatcgaa ccatctgaga aaagcaaatg ggcaaaatca taatcggtaa aggactcgat
    13741 gtgatccgcg atattttccg caactggtga gccttctttt cgaagctcat taattacttg
    13801 taataatccc cgttgattgc tttgggtaac cgtccgaatg gccttacgca acactggaaa
    13861 cttctcccca tctcgactag aaattcctgt taaaaaggtc agaatatcaa ttgctagact
    13921 ttcagaatcc ttctttttct ccataatcac ataaggatca agtaatcctt gattttttgg
    13981 cttactagtt aagttgagaa tattgatttc atccgcaatt tctggcaagg tttctttcca
    14041 gccacc
//
LOCUS       DACEQH010000067        13978 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.068, whole genome shotgun sequence.
ACCESSION   DACEQH010000067 DACEQH010000000
VERSION     DACEQH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.068"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(18..1247)
                     /gene="hflX"
                     /locus_tag="IW833_002242"
     CDS             complement(18..1247)
                     /gene="hflX"
                     /locus_tag="IW833_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ1308336.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1249..2166)
                     /gene="miaA"
                     /locus_tag="IW833_002243"
     CDS             complement(1249..2166)
                     /gene="miaA"
                     /locus_tag="IW833_002243"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ1308337.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2170..2907)
                     /locus_tag="IW833_002244"
     CDS             complement(2170..2907)
                     /locus_tag="IW833_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1308338.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2998..3525)
                     /locus_tag="IW833_002245"
     CDS             complement(2998..3525)
                     /locus_tag="IW833_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ1308339.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3705..4298
                     /gene="clpP"
                     /locus_tag="IW833_002246"
     CDS             3705..4298
                     /gene="clpP"
                     /locus_tag="IW833_002246"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ1308340.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4402..4887)
                     /locus_tag="IW833_002247"
     CDS             complement(4402..4887)
                     /locus_tag="IW833_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ1308341.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5040..5237)
                     /locus_tag="IW833_002248"
     CDS             complement(5040..5237)
                     /locus_tag="IW833_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308342.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5332..7092)
                     /gene="efrB"
                     /locus_tag="IW833_002249"
     CDS             complement(5332..7092)
                     /gene="efrB"
                     /locus_tag="IW833_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ1308343.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7089..8819)
                     /gene="efrA"
                     /locus_tag="IW833_002250"
     CDS             complement(7089..8819)
                     /gene="efrA"
                     /locus_tag="IW833_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ1308344.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9212..9424
                     /locus_tag="IW833_002251"
     CDS             9212..9424
                     /locus_tag="IW833_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ1308345.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9426..11105
                     /locus_tag="IW833_002252"
     CDS             9426..11105
                     /locus_tag="IW833_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1308346.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11359..11559)
                     /locus_tag="IW833_002253"
     CDS             complement(11359..11559)
                     /locus_tag="IW833_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1308347.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11709..11870)
                     /locus_tag="IW833_002254"
                     /pseudo
     CDS             complement(11709..11870)
                     /locus_tag="IW833_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12250..12323)
                     /locus_tag="IW833_002255"
     tRNA            complement(12250..12323)
                     /locus_tag="IW833_002255"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12287..12289),aa:Ile,seq:cat)
     gene            complement(12635..13033)
                     /locus_tag="IW833_002256"
     CDS             complement(12635..13033)
                     /locus_tag="IW833_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308348.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            complement(13026..13478)
                     /locus_tag="IW833_002257"
     CDS             complement(13026..13478)
                     /locus_tag="IW833_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308349.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            complement(13465..13971)
                     /locus_tag="IW833_002258"
     CDS             complement(13465..13971)
                     /locus_tag="IW833_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308350.1"
                     /translation="MDIKWLVQQNDSNLELAIKYLEETIFEDEHLTDNFLQVLKYLEI
                     YSVKKNKLIGENDSPIKTPIELSLRNRMGILQRSEIVKELFYHKFSYEIRLDDTYEHY
                     RIVFFVYNSIEDATATTALTFGFTKNGTINSDKTRQAATESDDICKKVCNGEENYWIG
                     EEKLNEIY"
BASE COUNT         4116 a         2943 c         2349 g         4570 t
ORIGIN      
        1 gttaaaaaaa taaaatttta ttggtttttt cttaaccatt ttgaattggt ttttgcaaac
       61 cctttaactt catattcgtt tgtttcttcc atgtacgctt cgtccaaaac cattgtttgt
      121 cttctcaact cgcttaattt ctgtccttcg ttgcttggca cagaaagtgt ataaggggtc
      181 attagttcca tcagctgacg tttgattgct tcgatcaacg ccatcttacc ttctttcgtc
      241 tgagtagaaa tcaatacatt tgggaatagc gtaggcgtga acatcgctgg gtcgatctgg
      301 tctgccttat tgtaaacagt cagtatcggc atttctttca tattcagttc atccatcaat
      361 tttaagacag tctgctcctg taaaatacga tctggagaac tagcatcgac tacatgtagc
      421 aaaagatcca tattttgact ttcttctaat gtagaatgga aagcatcgat cagttgcgta
      481 ggcaggtctt ggatgaaccc gacggtatcc gttacagtaa tctcaaatcc ttcagcaaaa
      541 cgccatctct tagtcaatgg atctaacgtt gcaaacaatt gatctttcga ataagtatcc
      601 gcttgtgtca atagattcaa gatagttgac ttcccggcat tcgtgtagcc aataagtcca
      661 atctgaaaaa tctcgctatt ttttcttttt tggcgattgc gctctcgatg agcttctact
      721 tctttcaatt ctttcttaac agcaaaaatt ttattccgta tatgtcggcg gtctgtttct
      781 aactttgttt cacctggccc tcttgttccg atcccaccgc caagacgaga taatgttttc
      841 ccttgcccta ctaaacgagg cagcaggtat tctaactgag caagttcaac ctgcagcttc
      901 ccttctttcg aacgagcacg catcgcaaaa atatctaaga taagctgcac gcgatcaatt
      961 accggaagac caacagcatc ggtaatcagt tggctctgtc tcggagttag ctcatgatta
     1021 aagatgatca aatccgcttc atgcgcatcc gcttgttgaa ctaattcttg taatttccct
     1081 ttaccgataa tcgtttgccg atccacttgt ggtctctttt gtgtaattgt gaatacgact
     1141 tctcctgatg cggtattcgt cagctgaact agctcttcca ttgactctga aaagtaacgc
     1201 tggtttgctt cggtttctac ccctacgatg atcacttttt cttccatttt attccgcctc
     1261 cagccatttt ttgattgttt gctcaatctg acttttttga tttggctcac ttataagatc
     1321 aaaccactgt gcatccaaac ggttacgaaa ccacgtcaac tgacgtttcg cgtatcttct
     1381 cgaatgtagt ttgacttgtt ctgttgcttc ttccaatgtt tctttccctt caaaataagg
     1441 gaaaaattct ttatatccaa taccttgact ggcctggact tctccaagct catacacctt
     1501 acgcgcttct gctactaaac cttctgctag catcaaatcg actctttgat tgattcgttc
     1561 ataaagaata ctacgcttcg tatctaatcc aatcatgagg taatcataca attgctttgg
     1621 ctcttcttta ggtgccgtga tactatgtcc tgtcgtctct aaaacttcca gagctcttat
     1681 cactttgcgt tgattgttcc agtggatttt ctcagcagct gccgggtctt tttctttcaa
     1741 ctgattccat aatgcttggg aaccaattgt ttcagctaac gtttcatact tttttctaat
     1801 ctcaggattc gcttcttctg tcgctcctaa ctgataatca tatagaaggg attggatata
     1861 gagacccgta ccaccagcta tgataggtag gtgccctcgg ttcgcaatct cggtaatcaa
     1921 ttgtcgacct gaatgctgaa aatcagcggc tgagtaacgt tgagtaggtt cgatcacatc
     1981 aatcaaatga tgcggaacat tattcatctc ttcttctttg atttttgctg taccgatatc
     2041 cagattttta tagatttgca atgaatcacc actgatgatc tctcctccaa atagttgtgc
     2101 gagttccaca cttagcgctg ttttaccgac tgctgtcgga ccgacgatca ctaatacttt
     2161 ttccatattt tacctacttt tcgttgattg tttccaccga agagctttct ccggaaaatc
     2221 agttattatt ccatctattt tgagttcttg acatttggcc atttcttctt catcgttaat
     2281 cgtccatgca cgaattgctt ttggaaaatc agctgcctgg actggatgtt cgaacaacca
     2341 agtatttttt ggatgtactc cttctatata aggggtttct gctgctagtt ggatcttttt
     2401 atcagaagta ctcatcaata agtccagctg tatctccggt tccagtaatg cgattttttc
     2461 caaagaatcc aaatgaaagc tgcaatatgc atgttgaaaa ggccaatctc tactttttaa
     2521 tgtttcggct aatttttctt cgataccggg atattcatac tgatcagttt taatttcaat
     2581 cattagcatc cctcggtaat ttttttggag gagcagtcct aacacttctc tcaattcagg
     2641 aactttagct gctggaaatt ctttgctgaa tttgccccct gcattcaatt ttttgagttc
     2701 ttccaatgtt ttttctcgaa tcagcccttt accgttagtc gtccggtcca cttcttcgtc
     2761 gtgcataacg atcaaataac catctttgct tagatggaca tctagttcga taccatctgc
     2821 acctactcgt acagcttctg caaatgcagg aagcgtattt tctgggtggg tgccgctgct
     2881 tccgcgatgg gcatagattt gtaacatctt tcttcactcc gttcagttct attgtatcat
     2941 taagaaaaaa agaaagcatt accacgctct catgtgcaac ggcaatgctt caaaatatca
     3001 gctccaaaca tcatctttcg agaagaaacc aatcggctgg cgcgtcatag tcttcaactg
     3061 agcttttttt tcattgatca tttcagtcag actatccgta aaattctcta aaatcacgcg
     3121 tccgccttta taagcatatc caccaatctt cattgtttca tctggagata aaatcacttt
     3181 tcgatgggag atattcttaa atagctcgtc taaaatataa tttggcagat aaaaactatt
     3241 ttgcgctgaa aatgattgga gatacgcaaa atcgtcaatc gtgatataac aatcatcaaa
     3301 tgcatcaaag ctgacatatt cttccatcag cgttgtccgt ttttgcaaga tctcgttgat
     3361 ctttttattt aattgattca gcgtctcgat ttctttgact ttcaattcca attcacgtct
     3421 acgctgttct ttattttcca agtgacgcat ttgcagtatc atgccgatat agacaactac
     3481 agcacctgtc agcactccga taaggccagt aataccaact atcaatctgt tgttcctcct
     3541 tgttttgttt ctctgcttat cataccataa agcgggttta tgcgactaat ttgcctaagt
     3601 tttttatttg accttttttg accaaaaatg tatactacat tttaggtatt agaaactaaa
     3661 ttatggtaca ctatttctaa acgaaatcaa ggaggaactc tattatgaat ttaattccta
     3721 cagtcattga acaatcttca cgaggtgaaa gagcttacga tatctactca cgtttattga
     3781 aagaccgtat catcatgtta agcggccaag taacagatga tcttgctaat tcgatcatcg
     3841 cccaattgct tttcttagac gctcaagatt ctgaaaaaga tatctatctt tatatcaatt
     3901 ctcctggtgg ttcagttaca gcaggtatgg ctatttacga cacaatgaac tttgtcaaag
     3961 cagatgtcca aacaattgtt atgggtatgg ctgcttcaat gggaagcttc ttgctgacag
     4021 ctggtacaaa aggtaaacga tttgctttac caaatgctga gatcatgatc catcaacctc
     4081 ttggcggcgc tcaagggcaa gcaactgaaa tcgaaattgc ggcacgccat attttgcaaa
     4141 cacgcgaacg cttaaacaaa attttagctg aacgcactgg acaaccgctt gaagtgattg
     4201 aaaaagatac agatcgtgac aactatatga cagctgaaca agcaaaagca tatggattga
     4261 tcgatgaagt catggaaaac agttcttcat taaattaatc tgtttcaaag gaagcttttt
     4321 taagctctct tatctaaacc aaataaaaaa ggatggtcaa ctcaaactga ccatcctttt
     4381 ttattttatg gatgctactg cctatctttg ctccttgaat tttccttgcg catgcatccg
     4441 acctatctcg acataatgct ggatgttttc tttgtttttt tgtatttctt cttttgtcaa
     4501 aggacggatc actttagctg ggcgaccaaa agccaagaca ttaggaggaa taacttttcc
     4561 ttctgttact aatgatccag caccaatcaa actattctca ccaataaccg catgattcaa
     4621 tatcgtggtc cccatcccga tcagcgcccc tttttcgatt gtacagccat gtagcatgca
     4681 ctgatgtcct accgtcacat tctccgcaat atctacaggt gcatcatgat cgacatggat
     4741 gattgtacca tcttggatat tcgttctttg tcctattttt atccaattac tgtccccgcg
     4801 taaaacagct tgataccaga tcgtcacatc ttctgacaat gtcacatcac caatcactgt
     4861 ggcatttgat gctataaacc gttccatatt tatttttctc ctttcatttt ttgtagaaac
     4921 taatcctatt gaataaaggt atcatagcat atatttattt cataagaaaa aaaacaggta
     4981 aaacctgctc aggccagatg tctatttaca gtatctggta actgacgttt tacctgtttt
     5041 tattgtacat ctatattttc ttctattact ttttttaaaa ttaattcgta cacttctatt
     5101 tgtgctgcca agcgttcgat ttgaaactca ttttcaaaaa aatcagctga atcaaaaggc
     5161 ttcgtctgac agacgtgcgt taaacgatca acttctcttt tggcttcagt aattttttca
     5221 tttaaaaact gttccatctc cgtgtcctca cttttcttga taactttact ttaccattat
     5281 caagaataga gaacaaaaaa taagttttaa actgttttat tcttatatga cttattctgc
     5341 ttcctcagca aattggctgt tatacagttt ttcataaaat ccaccttgtg ccaataaact
     5401 ctcatgcgtt cctttttcaa tgatctgacc ttgatccatc actagaatca gatctgcatc
     5461 acggattgtc gataaacggt gagcgatgac gaaacttgtc cgtccctcca tcacgcgatc
     5521 cattgctttt tgaatcaacg cttctaaacg ggtgtctact gaacttgttg cttcatctaa
     5581 aatcaagatt ttaggatcgg agaccactgc tcgggcaatc gtcagcaact gtttttgtcc
     5641 taaagacaca ttgtctccct cagaattgat ggccatatca tatccatctg gcatcgttcg
     5701 gataaaatga tctacgtttg ctgttttcgc agcatcaact acttcataat ctgtcgcatc
     5761 aagctttccg aagcggatat tatctgcaat cgttccttga taaagccatg catcttgcaa
     5821 gaccatacca aatagcgaac gcacatcact gcgactcatt gatttggtgt cgattccatc
     5881 aatccttatt gctcctttgt ctacgtcata aaaacgcatc aatagattga tcaatgtcgt
     5941 cttgccggca ccggtcggac cgaccacagc tacggtttga ccagctttca cttcaaaatt
     6001 caaatcttgg attaatggtt tcttcggatc atatgcaaaa tcgacatgat cgaatgtcac
     6061 atctcctaaa atcgtttctg gcagtgaagc atctactttg ttttcttttt cttctggctc
     6121 atctaaaatc tcgaatacac gtttcgttgc cgctgatgca ctttgcaaaa ctgaagacaa
     6181 ttgtgtcacg ttccccattg gctgactgat ttgccaaata tattggatga atgcttgtag
     6241 ttgaccgaca acgatcgccc cagtaataac ataaaagctt cctagaacag ccataccaat
     6301 ataagtagca taggcagtta attgaactag aggcatcatc aagccagaaa tgaatgctgc
     6361 acgaaaccca aagtatttca agctgtgatt aacttttttg aatccttcca atgtttcttt
     6421 ttcgcgtcca tatactttca atacactaaa acctgtcata ttttcttgga cgtagccgtt
     6481 tagctcacca agagaattct gcatgctttg aaaatatttc tgcgaagcat tgatcactcg
     6541 ttttgagatc cagatcgatg ttggaatcat gatcatagag atcagcgcca tcatcggctg
     6601 gatataaaac atcattgctg cagccaacgt aattcccaat atcgcattta cgataccaat
     6661 aaaagcttgt tgtaaagcac cgctgactgc atctacatca ttcgtcacac gagacaaaat
     6721 atttccttgc tggtttttat cgaaatatga aaccggaaga cgattgattt tttcttctat
     6781 gtctcttctc aaatcatgca tggcactttg aacgacattt gtcatcaaaa caccagaaag
     6841 taattgcgaa atacaatacc ctattccaac aactgtgatc caaatgagac atttgaatat
     6901 ataagaaaaa ttgattgatt cgccattggc aatattacgg ctgatttctg tagtaggaag
     6961 tcctgtaaca tatggcaaag ccacgttgaa agccactgtc agaactgtaa ataggatcac
     7021 taaaagaaaa gtctcacgat acggtctgat atatcgagct aaacgtttta aggaagaaaa
     7081 ggtttttttc atgctaattc ctcctttgat aattgagaag cggcaatatc ataataaatc
     7141 gggcattttt ccaacaactc ccgatgcgtc cccataccga cgacttctcc ttcatttaat
     7201 acgatgatct tatctgcatg catgatcgtt cccacacgtt gtgctacgat caatacagta
     7261 gattctgtcg tttctttttt cagacgagca cgtaacttcg catccgtttg ataatctaat
     7321 gcagaaaagc tgtcatcaaa aatatagatt tccggacggc gtatgatggc tctggctatt
     7381 gccaatcgtt gtttttgtcc acctgaaaaa ttggaaccac cttctgataa atgttcatta
     7441 aatccatctg gttttcgaga aatgaattct gaagcttgag caatctcagc tgcacgttcc
     7501 atttcttctt gcgtagcatc ttctttcccg tatcgcaaat tatctgcaat cgttccagta
     7561 aatagcagcg ctttttgcgg aataaaccca atcttatctc gcaacttgct tagccgataa
     7621 tctctcacgt ctactccatc gatcaagatc tttccttcac tcacatcata aaagcgaggg
     7681 atcaattgga tcaacgtaga ttttccgcta ccggtacttc caataaaagc aacggtctct
     7741 cctggagagg ctgtaaaact gacatttcgt atcactggct cttgcgaatg gcctggataa
     7801 gcaaatgtta cattttgaaa ttcgatatac cctttagttt ttgtatcaga tataccattt
     7861 ggatcttcta caatcgaagg tttggcatct agggcttctt ggatacgtcc agctgaaaca
     7921 gctgctcttg gatacatcat gaacacacta gcaaataaca tgaatgagaa caaggcatgg
     7981 aatatatact cgataaacgc aatcaaatct cctacacgaa gctcgcctgc atcgatttgc
     8041 actgccccgc tccagatgat caataccatt acgatattaa ataagaaata aaaacccggt
     8101 tgtgcaacag ccatcaaacg gaataagctt tttgaactag aagcataatt ctcattgact
     8161 acagcaaaac gtttttcttc aaacttttca ttgacgaatg cacgaatcac acgcaatcct
     8221 gaaagatttt cccgcaagat actgttgatt ttatctaagt ttttttgttg ctttttagat
     8281 aacggttcag acaccttagc aatcgctacg actgccaata atagtaaagg taatgcacct
     8341 actacataca aactgagtgt cacacttgta cgcatgacca tgtacaaact gacaatgaac
     8401 atcattggtg ccataaaacc gatacgtaaa atattttgca ggaacaacat gatctgataa
     8461 gcatcgtttg ttgtacgagt gatcaatgaa gatacgccta gattttcgta ttcctgatgc
     8521 gaataacttt ggatcttttc gaacaaatca tctcgaatat ctgctacgat attagtcgta
     8581 atcctcgaac caaaataacc taacaagata ttcaaaataa taccgattac agtaataacg
     8641 atcataaata tcccttgttg cttcacataa tcaaaatcat cattgcggat tcctacatcg
     8701 atcatacgtg ctaaaatcgt aggaagtcct aattcaatca aaataaaacc aaatacgcat
     8761 atgaaattga aaaacaagaa tttcttataa cgcatggtat aatgccacat tagtttcact
     8821 ttctcatctc cttttaatct tttgttacat tttcattact aaaaatgata gcacaacgag
     8881 cctattaata gaacacttct ctcataaaaa agtcattatg attatagcgg gatgataaac
     8941 attgattttt tctttcaatc tagttgattt ctatacttat ttgctgtttt atgctagaat
     9001 aacaattaat tgttgtatat tttttataca gaaggtacgg ttgaaaaaat atccgaacag
     9061 ataagcaaaa acgaggcttg tctctttgac tcgaatccag tattctagtc aaagcatatg
     9121 aaaaaccagg tggcttgtcc cctcgttttg tgttgtctaa aaaaatcaac tagctaaatc
     9181 actgctttac gcagataaag gagacaaaac aatgatctat aaagtttatt atcaagaaac
     9241 aaaagtacgc aaccctaaac gggaagaaac aaaatctctt tacattgagg caaaaagcga
     9301 tgtagacgct cgccaacgtg tggaagaaaa taccccatac aatattgaat tcgttcaact
     9361 tctagaagga agtcatctag agtacgaaaa agaaaatgct gatttctcat tagtggagtt
     9421 ttaatatgaa actttccatc aaaaataacg agacaggcgt ctttgctatt ggtggattag
     9481 gtgaaatcgg gaaaaatacg tatggggtac aattccaaga tgaaatcatt ttgattgatg
     9541 ccgggatcaa attcccggaa gacgatttac ttggaattga ctatgtcatt cccgactaca
     9601 gctacatcat acaaaactta cacaaagtaa aagccttggt gattactcac gggcacgaag
     9661 accacatcgg cggaataccc tacttgctga aacaagcaaa tattcctatc tatgctggtc
     9721 ctctcgcatt agcgttgatc acaaacaaat tagatgagca tggcttgctt cgagatgcag
     9781 agctccatga gatcaatgaa gatacgatca tccgttttag aaagacttct gtcagctttt
     9841 tcagaacgac ccatagtatc ccagatgcat tagggatcgt ggtgaaaaca ccttctggaa
     9901 atatcgtagc gactggagac ttcaaattcg atttcactcc tgttggcgaa ccagcaaact
     9961 tgcataaaat ggctcgtatc ggggaagaag gagtcttgtg ccttttatct gatagtacaa
    10021 atgctgagat tccaacattt actaaatcag aaaaaacgat tgggcattct attttgaaga
    10081 tcttttcaaa aatcgaagga cgtatcattt ttgccagctt tgcttctaac atcttccgtt
    10141 tgcagcaagc agcagatgcc gctgtggcaa ctggtcgtaa aatcgctgtt ttcggacgaa
    10201 gcatggaaaa tgccatcgtc aacggtcaac gtttaggata tatcaaagtt ccagacggca
    10261 catttgtaga tgcacacgaa atcaatcgct tgcctgccaa tgaaacgatg atcctatgta
    10321 caggttctca aggagaagcg atggccgcgt tgagccgcat cgcgaatggt actcaccgtc
    10381 aaatccaaat ccagccagac gatacagtga tcttttcaag ttcacctatt cctggtaata
    10441 caacaagtgt caatcatttg atcaatttgc tgtctgaagc tggtgcagaa gtgattcacg
    10501 gaaaaatcaa taacatccat acttccggtc acggtggaca agaagaacaa aaattaatgc
    10561 ttcgtttgat gaagccaaaa tattttattc ctgtccacgg agaattccgc atgttgaaag
    10621 tacatgcttc tctagcacaa gatgtaggtg ttcctgcaga aaactgcttc atcatggaaa
    10681 atggcgatgt tttagcgtta actagcgatt ctgcgcgtcc tgctgggcat tttgtggctg
    10741 gagatgtgta tatcgatggt agcgggatcg gtgatattgg taatgtcgtt ttacgtgacc
    10801 gtcacctgct ttcagaagaa ggtcttgtat tagctgtcgc gacagtggat atcaagaaaa
    10861 aagaaatctt ggctggtcca gatattctct ctcgtggatt tgtctacatg cgagaatcag
    10921 gagaaatgat caatgaagct cagcgaatct tattccacgc tttaagagaa tgtttgaatg
    10981 gacctgactg ttcagaatat aaattgaaag aagcgatgac tggcgcgctt cagcctttct
    11041 tattcgaacg tacagaaaga catccaatga ttttgccaat ggtaatgaca ccagatgaag
    11101 aataaataag aggttatgaa atcagcacct tgtcctgaat ataagagaaa acaaatgaaa
    11161 aacatgagaa gccatttttg tttgttctta ttccttgata caaggtggct ctttcatagc
    11221 ctcttaaatt tatttacttt ttcatacaaa aaaatacctg ccacaagaga aattgtctct
    11281 tgtggcaggt atttttgtta ctcgctttgt tctctcgtaa aaattgatgt ctaccatcaa
    11341 tttgatttac agataaaatt aagcttttac aacgtttgca gcttgtgggc cgcggtctga
    11401 ttcttcaacg tcgaaagtca ctgcttgacc ttcttctaat gttttgaatc cgtcaccttg
    11461 gatagctgag aagtgaacga atacgtcgct tccatcttca cgtgagataa atccaaatcc
    11521 tttttcagcg ttgaaccatt ttactgttcc gttttccata ataatatcct cctcgtgcca
    11581 aaagcactaa tttaaattgt aacattgctc gtgtacgata gaggtgcaac agatgtttca
    11641 acttctattc ccaaacaaaa ttacaatata attgtacact tgcttcattt tttttgcaag
    11701 aaagcaactt acttctcttc catcattcct tcttctctca aactaaaata tcgtttgcgt
    11761 ccgatgatca agtggtccag tacagtgatc cccatcattt ctccacactt ttgtatcctt
    11821 ttggtaaaat tcatatcctg ctgcgatgga ataacattac cagaaggata atataggtta
    11881 gtacacgtat aaaaatatct tttatattga cacaatttat ttaacaagta taataataat
    11941 tgaaagtgaa tttaaacaaa tatacaccat aaacacaacg tacctgcgaa tacgttgtgt
    12001 ttttttcttt ttcaaaaaaa gacgcttacc gctgcgaaac agtaagcgcc attgtcggct
    12061 ctaatgttta tctagccaat gactaaataa tgccaaagtt aaaccaatta acaatggcaa
    12121 gagcacgtgc tccaaagtga attcaaacaa atcatacacc tcctcccacg aaaatctctg
    12181 tggaagcgac gagccgacat tttattatac cattattgaa ttttattttt tatcaaatag
    12241 attaaattat gtaccctgta ggactcgaac ctacgaccgg acggttatga gccgtctgct
    12301 ctgaccaact gagctaagag tacaggttgt tgccacataa agccataaac aatcaaccag
    12361 tagaaggtgt ggcaacaatc ctgttatcgc atagcttgga gtgtgactat tatgggtgat
    12421 agtgaagata tgcgataaca tcactatttt atcgaatgat ttttatagtt gtcaatacag
    12481 tcatctaaat acttcatatc aatcatttat tcagaaacaa actatcttgc aggatctata
    12541 cgagttaatt acagagcaaa aaagcctcaa ccaaggatga ctggttgagg tctttgtctt
    12601 gcaaatttaa atagataact ctattacttg tattttattt ttgttgacta tatagctgat
    12661 ataaatcaat aactgtaggc ataactccta cttcgctata taaattagaa atcaaattag
    12721 aagctttatt caggataggt ttagctagct ctcccataga ttcttctact aattttttat
    12781 atggctgttc atttgtagaa acacgaataa ctgaaaagag taaaatatct accggtgctc
    12841 cttccatccc ccctacaatg tgagctcgta aagaaacaag ttttttttct agatactctg
    12901 cttcgtcttc tttatatcca agttcaatat taaactctaa atctccataa ctaaaattat
    12961 cagttaattc aaaatgaatt ttattcaaac taatattttc aagtgcaaaa gtaacactat
    13021 ttcctttatc catgtacata tacctctttt tctacaatat agcggctcgt atcttggtaa
    13081 ttaacactag catagaccat atttgacgat acttcttttc tctttctact tttaaatact
    13141 gagtaacttt tattccgaat ataaggttta ttttcaatac ggtcatattt aatagggccg
    13201 ccagcaacct taatagtgaa attcattgaa tctttatagg acaaattttc aacttgtttc
    13261 aaaagtttat tgtactctgc aacaggaata tgcatatctc cgaactcaaa ttcaagatat
    13321 tttttttcac ttattcctaa catttttgag aattctgtca cgtttttttg tttttttaat
    13381 cttattttaa gtaattgttt ggacaggttt ctttgttcag aatttaattt tttatataaa
    13441 tcaggatgtt tttttaataa atcattaata gatttcattc aacttttcct cccctatcca
    13501 gtaattctct tccccattgc atactttctt acatatatca tcactttctg tagcagcttg
    13561 acgggtttta tccgaattga ttgttccatt tttcgtaaaa ccgaatgtta atgcagtagt
    13621 cgcagtagca tcttctatag aattgtatac gaaaaaaaca attctataat gctcataagt
    13681 atcatctagt cttatttcat aactaaattt atgataaaaa agttctttta ctatttccga
    13741 tctttggagt attcccatcc gatttcttaa gcttagctca ataggtgttt ttattggaga
    13801 atcattttca ccaattaatt tatttttttt aacagagtaa atttcaagat attttaatac
    13861 ttgtaaaaaa ttatctgtaa gatgttcatc ttcaaagata gtttcttcga gatatttaat
    13921 cgccaattct aaattgctat cattttgttg aaccaaccac ttaatgtcca tctcacta
//
LOCUS       DACEQH010000068        13919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.069, whole genome shotgun sequence.
ACCESSION   DACEQH010000068 DACEQH010000000
VERSION     DACEQH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.069"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            133..507
                     /locus_tag="IW833_002259"
     CDS             133..507
                     /locus_tag="IW833_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1308351.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(621..1250)
                     /locus_tag="IW833_002260"
     CDS             complement(621..1250)
                     /locus_tag="IW833_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1308352.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLPLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1384..1456)
                     /locus_tag="IW833_002261"
     tRNA            complement(1384..1456)
                     /locus_tag="IW833_002261"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
     gene            1670..1743
                     /locus_tag="IW833_002262"
     tRNA            1670..1743
                     /locus_tag="IW833_002262"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1704..1706,aa:Asn,seq:gtt)
     gene            1698..1847
                     /locus_tag="IW833_002263"
     CDS             1698..1847
                     /locus_tag="IW833_002263"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308353.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2340..3668
                     /locus_tag="IW833_002264"
     CDS             2340..3668
                     /locus_tag="IW833_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1308354.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3870..4703
                     /locus_tag="IW833_002265"
     CDS             3870..4703
                     /locus_tag="IW833_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ1308355.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4719..5456
                     /locus_tag="IW833_002266"
     CDS             4719..5456
                     /locus_tag="IW833_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ1308356.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5611..6579)
                     /locus_tag="IW833_002267"
     CDS             complement(5611..6579)
                     /locus_tag="IW833_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ1308357.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6811..7644
                     /locus_tag="IW833_002268"
     CDS             6811..7644
                     /locus_tag="IW833_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ1308358.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7678..8517
                     /locus_tag="IW833_002269"
     CDS             7678..8517
                     /locus_tag="IW833_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1308359.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8543..9103
                     /locus_tag="IW833_002270"
     CDS             8543..9103
                     /locus_tag="IW833_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1308360.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9302..11023
                     /locus_tag="IW833_002271"
     CDS             9302..11023
                     /locus_tag="IW833_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ1308361.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11188..12330
                     /locus_tag="IW833_002272"
     CDS             11188..12330
                     /locus_tag="IW833_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1308362.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12346..13557
                     /gene="thiI"
                     /locus_tag="IW833_002273"
     CDS             12346..13557
                     /gene="thiI"
                     /locus_tag="IW833_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ1308363.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4752 a         2298 c         2834 g         4035 t
ORIGIN      
        1 gtctacactc tgagatgtaa gaaatatcat aagatatttc ttacatcttt ttgttttgag
       61 caggacttta gataatttta ttaatatatg ctagcatgct tttgtaagaa aaagaaagag
      121 ggcgtattag ttatgtatct taaaattacg gaacaagcac aagaaagatt acaaaaatat
      181 attgatgaag gcgcaacagt tatattggat ttagatgatg gcgttgggga atattctaaa
      241 atgggtgttt gctctttaga tactagtttt cgtttacttc ttcttgataa agaacagaga
      301 aaaaatgatt ataaactcca attagatagt gatattgggg atgtttatat aaaagattat
      361 tcaaaaatgt acatggatga acatatgacc ctttctttag atccacgtct aggtgtattt
      421 actcttgaaa gcccaagtgg ttctttagac agtcatgttc aattagtaga tctacgacca
      481 gaaaaattct ctggaataaa acaataactt tctctctatt gtccataaaa ctagtctcaa
      541 tcaagctgta ttgaaaaaac aagacaacat tcttaccatg gtaaataaaa agacgacaca
      601 ttttgtcgtc tttttttaaa ttaactattt tcgataaaag atcgcatagc ttgtgccatt
      661 atttctggct tttctctatt caattcatgt tttccgtttt taataaagag tagccggcca
      721 ttcgttgaaa gtttatataa acgttttgct gctggtttat tcgcatgatc tttggtaccg
      781 cataaaatca atttaggaag tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca
      841 agtgaacgaa gtaggggaag ggtttgtttt ttagatagac ctaatttttc aaatatctta
      901 gaaggtacgc agtaaaaata aatattctgg aaagtaagca ataagcgatt aggagactca
      961 aattgtggag cgactaaaac aacctttttt aaatatggag aaggagttgc aagatagttt
     1021 aaagcaataa cagcgcctaa agataatcca cataaaataa aaggttcctt tatctgattc
     1081 aattgtactt cgatgtattt ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa
     1141 tgattgaaca atgaaagact tttgcaagaa taactattgg gtagataaga gattacatct
     1201 ttccatgatt gttcgtcttg gcctagacca tgcaaaaaaa caattttcat attgctctcc
     1261 tttttatagt tatcatatag actttctttg tactattaaa atataaacat tcatttctta
     1321 cgtcataaaa taaaaaaaca gcgcagacac attgttaaac caacatgttg cactgtttaa
     1381 ggaagccact tgccgggctt gaaccggcga cctcttcctt accatggaag cgctctacca
     1441 gctgagctaa agcggcatta tcattaatac aaataatagg ataacgaata ttaatgtatt
     1501 tgtcaacaga attttttcga aaaaatgtgt ttctctaata ggaaaacaga ttttagaaaa
     1561 aataaaaata acaaaaaatg cgaacggttt ctttgataat ataataaaaa aattcaaaaa
     1621 aagtattgcg ttatttagaa gaaattgata ttatattcat gtacttgatt ccggcatagc
     1681 tcagttggta gtagcgcatg actgttaatc atgatgtcgt aggttcgagt cctactgccg
     1741 gagtaaataa agcttatgca aagaaaaaga aatccaatca cttgggtttc tttttctttt
     1801 attattatga tgaatcaagc tttctgtttt tgtcacgtat gttgtaatct ttacgaaaga
     1861 gtagagttgg ctactcataa tcaaccatac ttcccatcat cgctaaatac gaaataaatc
     1921 agcattccta caccaatcaa agcacaagct gcaccaagaa aagggatgac attcccaaag
     1981 ttatctgtat tcataagtat gccaccaccc acaaacaaaa agccgccagc aatcaggttt
     2041 ttcattttat tcatctcctt tcttacgtta tagtatttcc ttctttcaat gagtattttg
     2101 aaagctatct tattgtatga tgttcttcta ggcctcttct ttattataca ggccattttc
     2161 gttatctcat agaaatattc ctttttatct gaaaactatt gtataccgta tgacattttt
     2221 cttcatgaag ttgtaagtat attctcattc aaatgaaaat ttcatcatag aagagattgt
     2281 atttctaaat tcttaagagt aaaatacgaa gtataacttg gatttggaag gatgattgga
     2341 tgaaagctat tacgcctgat atgacaacaa aatttaacaa acagtttcaa gcaaatatca
     2401 aacagcaggc attgcagcgg gcagttgtga agaacggaat tactgcttct gctgaaaatg
     2461 tttcagcaca tgtggagaat acaccggtat tttctataga tttggctacg gggaaagtgg
     2521 ctaatcaaat gcaatcgggg cgttgctgga tgtttgctgc actgaacaca ttccgtcata
     2581 agatgctgaa tcaattccat ttaaaagagt ttgaattatc acaaaattac acttttttct
     2641 gggataaata tgaaaaggca aattatttct atgaaaacat cattgcaaca ggaaaagagg
     2701 caatcgatag ccggaaagta gctttcttac tcgctactcc tcaacaagat ggcggccaat
     2761 gggatatgat cgtttcatta ttccaaaaat atggcgttgt gcctaaatcg gtgatgccgg
     2821 aaagtgcgaa tagttcaaat tctcgagatt tgaataacta cttgaacaaa ttactgcgta
     2881 aagatgcagt agtgttacgg aaaatggttg ctcaaggaga aacattagac gctattgaag
     2941 aaaagaaaga agaaatgcta gaaagcattt ataacttctt agctatctca ttaggaacac
     3001 ctccaaaaga attcgatttt gagtaccgcg atgaagaaaa gaactatcat cttgaccgag
     3061 gactgacacc acaaattttt tatgataaat acatcggtgt aaaattagat gattatgtta
     3121 gcgtgatcaa tgcaccaaca aaagacaaac cattcaatcg ttcttataca gtagagatgc
     3181 taggaaatgt cgttggcggg aaagaagtca aatatctgaa tgttgatatg gaaactttca
     3241 aaaaattagc gattgctcag cttgaagaag gagaatctgt ttggtttggt tgtgatgtag
     3301 gtcaatcttc tactagaact agtggcatca tggccttaga tgcttattca atggatgatc
     3361 tatttgatac ggattttaca atgactaaag ctgaacgttt agattttgga gaaagtctga
     3421 tgacccatgc gatggtattg accggtgttg acttagtaga cgggcaatca acaaaatgga
     3481 aagtcgaaaa tagctggggc gaaaaagttg gaaagaatgg cttctttgtt atgagtgatg
     3541 cctggatgga cgaatatacg taccaaatcg ttgtaagaaa agatttattg acgaaagagc
     3601 aattaaatgc gttcaatgaa gaaccaattg ttctatctcc gtgggatcca atgggtgctt
     3661 tagcatagac gagtatttca atgaagagta tgtagtaaag ataaaaagag ctggcttaag
     3721 ccagctcttt ttatctttaa agtccattcc ctgtttttta tggtccctgt gctataattc
     3781 taaataaagg ataaggattg gaaataatcc ttttatcatt aaaaatatga tgacgaagta
     3841 gaataaaatt gggagtaggg gggaaagtca tggatatcca aactgtttta tctgatctga
     3901 aattaaatgg taaagtgatt ccagttgttg gtggcgatgt taatcagacc tatcgtataa
     3961 aaaccgaaca tcgtgcttat tttttaaaaa tccatccgaa tgtaaaaaaa ggattttttg
     4021 aagcagaagt agacggatta aaagaactgt cagcattcgt acgtgtaccg gatacgtata
     4081 tgctaggtga aacaagtgaa ggcgcttatt tgttaatgga atggatcgag ccgggaaaag
     4141 gggatcaacg agatcttgct gcagctttag ctaatctcca tcagcaaaca gcgccacaat
     4201 ttggttttcg taaagataac tatttgggta cacttgttca gaaaaattct tttgaagaag
     4261 attggtggac ttttttcttt aaagatcggc ttgaatccca gatttctttg gcagaagaaa
     4321 cgaatcgatg gaatgtacag cgtcaagaaa agtatctgcg atttaaagaa cgtgtattga
     4381 aatcggttga accaaaaaag atcactccac gcctcttgca cggagacctg tggagtggta
     4441 atgtgttctt tgaccagcaa gggcatccag tttttgtcga cccagctgtt tcttatggga
     4501 atcgggaaca agatattgca atgagccagt tattcggtgg tttccgaccg gaatttttag
     4561 atgcttatca aactatattt ccattagaaa agggatggaa agaccgactg ccgatttacc
     4621 agttatatta tcttttggca catttgaata tgtttggaga aagttacggt tcccaagtag
     4681 atcaattact agaaaatttt taagaggagg gcgtgttttt ggcaaaaaaa tataccaaaa
     4741 agcatgaagt ttcatattat gagtgtgaca tcaaccaaac gatgacattt ccttcgatgt
     4801 taggagtagt tatcaaaacg tctgaagatc aaagcgacca gttgggaaga ggcagtgaat
     4861 tcgtcaatag ctttggactt acttgggtca tcacgaatta ttccatggag attaatcgtc
     4921 tccctagagt cggtgaagta atctcagtaa cgactcaagc catggagtac aataagtttt
     4981 tctgttatcg gaacttctgg atccatgatg aagatgggaa agaattagtc aaaatagaat
     5041 ctgttttcgt attgatggat tttgtgaatc gaaaaatgag cagtgttaat gaagagatca
     5101 ttgctccttt tgagagtgag aagatcaaga aaatcaagcg tcaggaaaaa attgaaaaga
     5161 ttgattcggg cttcatgttg ccatatcgtg ttcgttttta tgatatcgat agtaatcagc
     5221 atgttaataa tgcgatgtat tttaattgga tcattgacgt attaggatat gactttttga
     5281 ctacgcatgt tccagaaaaa gtaattattc gatttgataa agaagtagag tacgggaatg
     5341 agatcgaaag ccattttgaa caaatcccta tcgaaaatgg tgtcaaaaca aaacatgaaa
     5401 tccgaatgca agaacagctg tattgtgaag caaatattat atggaaaaat aaataaaaaa
     5461 gaggttgtga cagaaggggg caacttcaag aaataagaag aaatttacga aaattgtttc
     5521 tcaaattttt gtaaattttc gacttagttt tcgaagaagc tatttttgtc acagcctctc
     5581 tttttaagtt tagctattag ataatgatgt ttagttttgg ataaacgaaa tcaattcttc
     5641 acccgacaaa ccatagttta cagcaatcaa tagttttaaa cgtgcttttt gactattaat
     5701 actgttacag aaaataatcc ccattttttc tagttctttt cccccgcctt cataatcata
     5761 aacaggctct gcgattccat tgaaacaacg agaaacaagt actacaggga ttttttggac
     5821 aagaagcttt tccaaagcag ctaaggcttg tggagggaga ttccccgcac ctaaagcttc
     5881 gattaccaaa ccgtctactt tcgtatgggc gatggcttcc aataagtccc cttgcatccc
     5941 agcatatgct ttaacgatcg ggatagtccc atctacagca gaaatgtcca gtcgctttgt
     6001 ttctaaaagt tcctgcaaga ataaaattcg attcttagtt actaagccga tgggacccaa
     6061 tgtaggtgtt cggaaagtag caacatttgt ggtatgggtc tttgtcacgt agcgtgcaga
     6121 atggatctca tcgttcatga ccaccaaaac ccccttatct agcgcttctt cacaacttgc
     6181 gacacgaatc gcactttcaa aattataaag gccgtcactt ccaagttcat tacttgaacg
     6241 cattgcccca gtcaaaacaa ttggtaattt gttgccaatc gttgtatcaa gaaaaaaagc
     6301 agtttcttct aatgtatctg taccgtgtgt gatcaccact ccggatatac ctgatgcata
     6361 agcggacaag atacgttctt ttaataccaa catgtgagaa ggcatgatgt gtggtgaggg
     6421 aagattgaag atatcttcaa ctaataattc tacatgcttg gggattgtga tttctgcatc
     6481 taataatggg tttgccacat caggactaac accgcccgtg agcatgtttt ctttcatggc
     6541 gatcgttccg ccggtgtgta gtacaagtat ttttttcatt ttacagactt ccttacgaat
     6601 aattttgcat agccgttaaa tcttattgta tactatataa aagagttttg ctatcagtga
     6661 agaataacga atagcctaac ttatcataaa aaagctgacc ttattaaaat ggaaaagact
     6721 caaccttgac ctcctaatac aatggagtat ctggttgagt ttcttttaca agaaagcagc
     6781 caaggaaatc aatcacgaag gtggccggaa atgatcagaa tcggattaac atcgtttaaa
     6841 gaacatgaca aattaactgg taaaaagaat aacactcttt atgaatatgc agggtttcta
     6901 cccttagtgg aattagatac tgcctattat ggaattcccc gaaagtcctc agttgagaat
     6961 tggataagtg aagtacctga atcatttcgc tttgtagtca aagtctatca aggaatcagc
     7021 ggtcaaggag actggaagcc ttattatcag accgaagaag agatgattgc gcatttttta
     7081 gatgttatgt cgccaatggt agatgctgga aagatgtttt gctttttagt ccaattcccc
     7141 tcatcattca aatgtacgaa agaaaatgtc cagtacttgc gcaaacttag aaagtggttt
     7201 gagggttatc ctgtttctat agaattgcgg gattattctt ggtacagcga tgaatttctt
     7261 gaacgtacaa gaaactttat gtctagagcc gctttttctt tagccatcat cgatgagcca
     7321 cagttgctga atacaacgat tccttttgat ccatttgtca ccaatccgaa ctttgctctt
     7381 tttcgattcc atggtaggaa tgcgcaagga tggcaagcaa atgataaaga gtggcgtaaa
     7441 aaaagaacgt tatatcgtta ttcagaaaaa gaactgcaag aactagcaca tgaagtcaaa
     7501 gtagttgatt ctaagacaaa agaggtaggt gtgattttta ataataattc tggtggtgat
     7561 gcggctgaaa atgcattagc actgcaaaaa atattaaata tcaagccaga tggactcaat
     7621 ccaacccaaa tggatctgtt ttaagggctt attttattca agaaaggggc gaagtctatg
     7681 atcaaagcta ttttttttga tattgacgga acattggtca ccaatcgttc aaaagcgttg
     7741 gaatcaacaa aacaagcaat tgaatatgct cggaaaaacg gcattttatg cggtgtagca
     7801 acggggcgca gcccagtaaa gatcaaagaa atcattgatg aattggaatt agatatgtat
     7861 gtcgtgtata atggtcaact ggtttttact gcagatcgca cgattatcga ccaccctttt
     7921 gaacaaaaag tattggaaca tattgttgaa tttgcagatg agaaccatcg acaaatcgtt
     7981 tttggtgcga gaaatcggtt agatggatca acgacgatgc tattaggaca atcgattttt
     8041 atcaagcgac tagtcagctt tttaccgcgt aaattcccgg tacgtctgat gaaaaaaatc
     8101 ctccaggttt ttagccctca ccgacaaaag gatcgttatg aaacgttaga gatattaaaa
     8161 gaaccaattt atcaatgtat attgctcagt ccggagtcag aacagaaaaa gttagaacat
     8221 cgctttcccg agtgtacgtt tcagcgttca aatacgtata ctgtagatat catcccaaaa
     8281 ggtggttcaa aattactagg catccaagct ttcgcaaatg ctgtcgaaat tgaaatggaa
     8341 gaaattatgg cattcggcga tcattacaat gatatcgaga tgctgaaagg tgtaggaatt
     8401 ggagtggcca tggggaatgc tcagatcgaa gtaaaacatg ctgcagactt tgtgactcaa
     8461 tcaaatgaag aagatgggat atacgaagga ctgaaacact tcggtctgat ttattagtca
     8521 atgaaagaag gaaaaaatca gtatggaagc agagacacct tttcaaaaag tagaaacatt
     8581 ccacaaaact tttgatccaa gaagaccaga gatacccacg gcttttacag aaaatgaagc
     8641 tcatacacgt gctgcgttca aaatagaaga acttgttgaa tttgttgcag ctacttcacc
     8701 taataaagaa caactgcatg aagctgttat gctgctgcac gaagcattag ataaagcaga
     8761 agaaaaaata cttggtaaaa cacagtgggg aagtgatccg ctggttgaac aagtagatgc
     8821 actgacagat atcctttact ttacttatgg ttctttttca ttactaggaa tagtgcctga
     8881 tcgaatattc gaaatcgtcc atcaggcaaa tatggggaaa ttatttccag atggaaaacc
     8941 tcattatgac cctgtaacaa ataaagtgtt gaaacctgaa aactggcaga tggaatatgc
     9001 tccagaacct aaaatcgcgg cagaaatcca acgtcagatt caagaagcga ttgctaagaa
     9061 gaaatccgct gatataggca tgtgcacaaa tgaaaaagag tgatcagagt ttcactattt
     9121 ttgttatctt tatacgatgg aaaaacaaaa gattttcgtt taagttagtg aatgcatgaa
     9181 aatagtatgc tataatctag aatagatgtc ttttgcacga gagacgtatt atttggattt
     9241 atgccaagca gaacgccatc tgttgcataa aagatgcata ctgaaaacgg aggaaccata
     9301 aatgggaaat aatgttatca tcggaatcgt cattgcaatc attatagtcg ccattgtcgt
     9361 ctttttggtt agttacttga tgaagaaaaa aaaccaagat cgattggatg ccttagaaaa
     9421 acgcaaggaa gaattatttg acttgccagt gattgaagaa gtcgatgatg ttaaaaaaat
     9481 gcatctggtt gggcaaagcc aaaatacctt tcgtgaatgg agtcaaaaat ggaatgacat
     9541 atctacttcc tcgttcgctg aactagaaag tcaaattttc gaagtggaaa acctcaatga
     9601 aagttttcgg ttcttaaaag gaaaaaaagc agttgttgaa gcagaagcta caatggacca
     9661 aatggagaaa caagtgacgg aaatccgtga aggtctaaag gaattgcggg aaacggaaga
     9721 acgcaactca ctagctgttc agcaggcact tgacgtctat gaagaactga aagaaaaagt
     9781 cagagaagac gagcatgaat acggaccagc tctttttgaa ttgcaaaaac agatcaaagt
     9841 gatcgaaaaa gaatttacgc agtttgtgac tttaaacact tctggagacc ctgttgaagc
     9901 ccgcgaggtt ttggataaag cagaaaaaca tacgtatgaa gtggaagctc tgatgaaaaa
     9961 aattccaccg ctgtatgagg agctccatac cactttccct gaacaacttg aagaaatcag
    10021 tgatacttac gagaaattga tggaagaaca atatgtattc ccagaagaag atcttgctga
    10081 agatatcgca aaagtaagtc gaagaattga aaattcatta gccaatcttg aaaaaacaga
    10141 agttgaaacg gtcgaatttg aaaaccgtga aacagctgat ttgattgatt cgttatatga
    10201 tatcttagaa cgggaaatgg aagctcagag atatgtaaaa actaaccaat caacgattgc
    10261 agagtatatc aaacatacaa caaagaataa ccgtcaatta ttgattgagc tggatcacac
    10321 atctcaaagc tatacattga accacaatga aattggccgt gtacgaggtt tccaaaccga
    10381 agtggaagaa atggaacgtc aaaatgaaca gatgattcca cagatccgtc aacacgaaat
    10441 tccttattca gaagtgcgca cgttctataa aacagtcttc aaagtcttag aagatatcga
    10501 aaagcaacaa gtagagatcg acgactcatt acatgaattg agaaaaggcg aaaaagaagc
    10561 gcaagaaaag atcgatacat tcgaattcaa attgcgcagt ttgaaacgtt ttgtagaaaa
    10621 acaacgtttg cctggcttgc cgaatgatta cctagaattc ttttttgttg ctactgaccg
    10681 tattgaagaa ctaagtgttg tcttgaataa aattcgagta aacatggaag aggtcaatcg
    10741 tttagttgct ctatgtgaag aagatctaga attattagat aagaagacac atgatttagt
    10801 agatgcagca gcattaacgg agcaaatgct ccaatatgca aatcgttatc gtcatacaca
    10861 cgaagaagtg agagaagcaa ttgataaaag ctactattta ttcaataaag aatatcacta
    10921 tcaagaagca ttagacgaaa tcggtacagc attagagcga gtagaacctg gtgctttcaa
    10981 acgcatcgaa gatttttatt ttaatcatcc agacttagta taaagcaaaa aagttagtaa
    11041 accagcaaga gcaagacgga ttattgtctt gttcttgtct ttttttgtat atttgaaaat
    11101 tgaaacaagt gaaaatttca cctgctcttg aaagcacctt ggacaaccgt tataatagaa
    11161 aagaatttca aaaaagaggg gtttcttatg atatattttg ataacagtgc aacaacccca
    11221 atctttccgc aaagcttaga tgcatatgta aaaaccagtc agcggattat tgggaatcca
    11281 tcaagcttgc atgatttagg caatcaagct aaccggttgt tgcagcaagc aagaaagcag
    11341 atcgctgatt tgatccatgt agaaccagaa gaaatctatt ttacttcagg cggaacagaa
    11401 ggagataact gggtattaaa aggaacgatg atcgaaaaaa gagaatatgg gaatcatatg
    11461 atcatttcag gagtcgaaca tccggccgtc agcgagacag cagaacaatt aaaagaatta
    11521 ggaatcgaag taagtatcgc cccagtggat aaacgcggat ttgtacgtgt cgacgaattg
    11581 aaagaattaa taagaaaaga gacagtatta gtctctgtta tggctgtgaa taatgaagta
    11641 ggatcgattc agcccatcca ggaaatcagt gatcttttgg atgattttcc taaaattcat
    11701 ttccatgtag atgctgttca ggcgattgga aaagtaccat tctcgcaatg gttgacagaa
    11761 agagttgatt ttgcaacatt ttcagctcat aaattccatg gcccacgggg aactggtttc
    11821 atttattgga aaaagggtcg tcgtttagca ccattactga caggtggagg gcaagaaaga
    11881 aatcagcgaa gtgggacaga aaatgttcca ggtattgtgg ctatggttaa agctttacgt
    11941 ttatcattag agataaagga acaaaaacct gagcagacac aacgcttaaa agtagctcta
    12001 atcaatgcgt tgaagacgta tgagaaagtg acgatttttt cagaaggaac tgactatgca
    12061 ccgcatattt tatgttttgc tctaaaagga attcgcggcg aagttctcgt ccatgcctta
    12121 gaagaaaaac aaatatttgt ttctactact agtgcctgct cctcacgtaa acatatggca
    12181 agcagtacat tgcacgcaat gcatgtacca aatgatctgg ctacatctgc tgttcgaatc
    12241 agcttagatg aaaacaattc gcttgcagaa gtcgagcaat ttatgattat tttcaagcag
    12301 ctttataaga aattttctgc aatcaattaa aagaggagtg ttcttgtgaa atacagtgaa
    12361 attatggtcc gttatgggga actttcgaca aaaggaaaga atcgaaaaag ctttatcatg
    12421 caactagcac aaaatgtcag acaagctttg aaagactttc cagaaatcaa aatccatgct
    12481 gatcgggacc ggatgcatct tttattgaat ggcgcagacg ggcagctagt tattccaaaa
    12541 ctagcaaaaa tatttgggat ccaaaatttc tctccaagta ttcgtgtaga aaaagatgtc
    12601 gaagttttga aacaagctgt tcaagacatc atgaaagaaa tttatactgg gaaagaaaca
    12661 tttaaaatta tcgctaaaag aagcgatcac caatttgaat tgacaagtaa tgaattgaat
    12721 caaacattag gtaatgctgt ttttgatatt ttcccgcata tccaagttca aatgcgtcaa
    12781 ccagatattc cattacgtgt agagattcgg cgtgatggcg cttatctatc gtatgaaacg
    12841 atcaagggag ctggaggatt gcccgtagga acaagcggaa aaggaatgct tatgctttct
    12901 ggggggattg attctcctgt agcagggtac tttgccatga agcggggagt ggaaattgaa
    12961 gctgttcatt ttgctagtcc gccgtataca agtgaacaag cactgcaaaa agcaaaagat
    13021 ctaacagcca aactagcacc ttatgtggga acgatccatt ttatcgaagt gccatttacc
    13081 gagattcaag aagaaatcaa aaagtcagtg ccacaaggat attggatgac aattactcgt
    13141 cgaatgatgt tgcgtttgac cgatgaaatt cgccgtattc gtcatggttt agtcattttg
    13201 aatggtgaat cattaggtca agtggcttca caaactttgc aaagtatggt ggctatcaat
    13261 gaagtaacga atacgccaat cattcggcct gtagcgacaa tggataaact tgaaatcatt
    13321 gaagtagcag aacagatcga tacatttaat ctttctatcc agccttttga agattgctgt
    13381 acgatttttg ctcctccaca gccaaaaaca cgccctcaac tggaaaaggt ccatcaatat
    13441 gaagagcgtt tagcaataga tgaaatgatc caacgagcac tagctggctt gaagatggaa
    13501 gagatacaag cagaatcgtc taatgaaaaa actgaagaat tttcagaatt gctataagag
    13561 gttatgaaaa tagtgttttg accagagtat tagagaaaca aaacgaaaaa atagctccat
    13621 gtattttacc gtttttgaac taatgtcgaa gattagctga ttgaatacca tttatcaaga
    13681 agttatggaa aagccggttc ttttttacta actgaaaaat aaaattttat ttagagatca
    13741 agctacagtt gataaatgat ataaaaaaga gaatttccca tgaaatagcg aggttgtgac
    13801 agaagtgggc agcttcaaga aataagaagg aatttacgaa aattgctttt caaatttttg
    13861 tgaaatttcg gcttattttt cgaagaagtt acttctggaa caccgtgtat cagaggttg
//
LOCUS       DACEQH010000069        13537 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.070, whole genome shotgun sequence.
ACCESSION   DACEQH010000069 DACEQH010000000
VERSION     DACEQH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.070"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(60..1007)
                     /locus_tag="IW833_002274"
     CDS             complement(60..1007)
                     /locus_tag="IW833_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ1308364.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1197..1466
                     /gene="rpsN"
                     /locus_tag="IW833_002275"
     CDS             1197..1466
                     /gene="rpsN"
                     /locus_tag="IW833_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1308365.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1642..2004)
                     /locus_tag="IW833_002276"
     CDS             complement(1642..2004)
                     /locus_tag="IW833_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1308366.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2015..3136)
                     /locus_tag="IW833_002277"
     CDS             complement(2015..3136)
                     /locus_tag="IW833_002277"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ1308367.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYIHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3152..3502)
                     /locus_tag="IW833_002278"
     CDS             complement(3152..3502)
                     /locus_tag="IW833_002278"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ1308368.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGKVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3632..5143)
                     /locus_tag="IW833_002279"
     CDS             complement(3632..5143)
                     /locus_tag="IW833_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1308369.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQSETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5465..6817)
                     /locus_tag="IW833_002280"
     CDS             complement(5465..6817)
                     /locus_tag="IW833_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ1308370.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKDFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVPDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAVKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(6970..8046)
                     /locus_tag="IW833_002281"
     CDS             complement(6970..8046)
                     /locus_tag="IW833_002281"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ1308371.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8242..9987
                     /locus_tag="IW833_002282"
     CDS             8242..9987
                     /locus_tag="IW833_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ1308372.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDSVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLKKEEQTQASRKTEIVHEKEFGSFAFRASTSTEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10236..10703)
                     /locus_tag="IW833_002283"
     CDS             complement(10236..10703)
                     /locus_tag="IW833_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ1308373.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFCQALKKAIDQGLPSTEDSR"
     gene            complement(10687..12060)
                     /locus_tag="IW833_002284"
     CDS             complement(10687..12060)
                     /locus_tag="IW833_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308374.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGIMYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDAAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12381..12869)
                     /locus_tag="IW833_002285"
     CDS             complement(12381..12869)
                     /locus_tag="IW833_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1308375.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12891..13493)
                     /locus_tag="IW833_002286"
     CDS             complement(12891..13493)
                     /locus_tag="IW833_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1308376.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3863 a         2938 c         2458 g         4278 t
ORIGIN      
        1 ttttgtcttt gcccctacaa aaatgtttca gcttttttcg ttttttattc ctgtatttct
       61 tatttttcga atgacaggat gattttccca gcaggaggat gtccttcaac tagctgatgt
      121 gcttggatga catgcttaag ggaagcagac agattcattg aaataaaaac atgaaaggct
      181 cctttttggt aggctttgat tatgccagca aaggcttctg catctagata ttcctttctt
      241 ggaacaaacg attcataaaa gccttccttt ttttgccgca aatctagatc tgggaggtca
      301 tttaatgcaa catacctgcc gcctggtttc atgatctgga tgccggtttc tcctttgata
      361 cttcctttcg tcgcatcgat tacaaggtct gcctgatcag caaattgcag acctggattt
      421 gttttatcat aagcagcaaa tgctgaagct cctaattttt ttaccttttc ctcattttta
      481 ctggatgcac tggttaagat tcggattccc ttttcatgga gaagctggat cagacttgac
      541 ccgaccgcac ccgatgctcc tagtatcatt acagtatctg tcggctggat ttccgtcaaa
      601 tggttgatta gattataagc cgtaattcct ggcgtgacca ttccggctgc ttcttcccaa
      661 ctcattttat ctggaatttt tgctactttt gctgatggca acacgacttc ttctccgtaa
      721 gtcccgccga ctgcatggac agctactcga tcacctacat gaacagttgt gacatctgaa
      781 ccaatctctg tcacaatacc agcgccatca ttacctggta cataagggaa tttcaatgtt
      841 cgtatctctt tcatttctcc taaacgcaag gcaacatcat aaggattcac actgaatgct
      901 tttatttcta cacgaacatg tccagaaccg acttctctgg gatgagcatc gatttcttca
      961 aaaacatctt gtgcttctcc gtaaccgtta attgcaaatc ttcgcatggt caccatctcc
     1021 ctttttcttt aagtttacgt ttatgccttt attttgacaa aaaaaatgtc ttttcgtttt
     1081 ggcgcatgat tcaaaacaaa atagttgtct tttttcgcgt aacgtgttaa attatttatc
     1141 taaaagcgta aagattacga tttacgctaa taaaaaataa ttggaggaat ttgttgatgg
     1201 ctaaaaaatc aaagattgcc aaagcaaaaa aacagcaaga aacgattgaa cgctatgctg
     1261 ctttacgcca ggaattaaaa gcgcaaaaag attacgaagg cttgcgcaaa cttcctttag
     1321 atgctcgtcc agaacgattg aaaaatcgtg atttgatcga tggacgccct cgcggataca
     1381 tgcgtaaatt cgggatgtcc cgtataaaat tccgtgaatt agcacataaa ggtttgattc
     1441 caggagtaaa aaaagccagc tggtaatcca agtctggctg taatgtaaag gaggagggcg
     1501 catggaccac tccttggatc atcaaaaaag atacgttcgc ttcttctagc agaaatgctg
     1561 gtactagaag cagacgtatc tttttttacg cacagctggt ccttttttat atgtaaaggc
     1621 tgcatggtta cttcgctata tttatttagt aacaggagtc agtgcttcga tacccacact
     1681 aattttcaat gctcggtcaa cttctgccat aatgggtcga tccaaatggc agaccttttc
     1741 tttcaatctt gatttatcga tcgttctgat ctgctccaac aggataacgg agtctctttc
     1801 tattcctgtt ccagtagctt tgatcccaat atgagtcgga agcttaggtt tagccatttt
     1861 tgccgttatg gccgcaacaa tgatcgtcgg gctaaaatgg ttacctagat tattttgaat
     1921 aaccaaaact gggcgtgttc cgccttgctc cgatcccacg actggagaaa gatcagcaaa
     1981 ataaatatct cctcttttta ccataggatc ctccttattc ttgatattct cttggtatcc
     2041 gctgtcctaa tccgcaagcc acttcatagt ggatcgtacc taattgatct gctatatcct
     2101 gcatagtgat ttctttgtct gcatttttgc cgataagcgt cacttttgtt ccgacaggaa
     2161 attcttgagg caatcggatc atcagttgat ccatacatac acgcccaatc gtttcacagt
     2221 agttcccttc taccaataga gaaaaaccct gcatctttcg tggccagcca tctgcataac
     2281 cgatcggtat cgttccgatc cactctgctt caggagtgat atatgtttct ccatagccga
     2341 ttccttcacc tgctgggagt tttttgactt gaattaattc agaaaccagc tcaagtgctg
     2401 gctgcagtgg ataaacttcc ggtagtgcgt gcccagaggg attcagtccg tacattgcta
     2461 ctccataacg aatcatattg ccgattgttt catcatgcca taaagccgtt gcactattgc
     2521 tgacatgtat atatcgagga agttcgggaa gtttttttag cacttctata aagcgttctt
     2581 tttgcagatt ccaatatgta tcatctgcct gatctgctgt agaaaaatgg gtaaataaac
     2641 cttcccattc taatacacga ctttctttga tgaatgcagc tgcctctttt acttcttcag
     2701 gtgtacaaaa tccgatacga cccatcccag tgtctgcttt tagatgaatc gaaagaggcg
     2761 tctctagctt acttttctct aaaacagcag ctgctgcctg caaccattcc cgtgtacctg
     2821 cagttactga taaatcatga gcaacagcta cagtcagtgc gtcgtaagga accatattca
     2881 agataaggat gggctgagta aatccagctt ctctcaactc gactccttca tctaaatttg
     2941 aaacacaaaa gcccgaagct ccacctgcaa ctgctgcctc agctgtttca atagctccat
     3001 gaccgtatcc gtttgctttt actactgcaa acaattcttt tccttgcgga agtcttgcaa
     3061 cttcatttgc tacattttca gttatagctt ttttatgtat cactgctttc gttgggcggt
     3121 gccaagcgac aaccatcgtt tttccttctt tctagtcttc caaaataatt tgtgcaaaag
     3181 ctgtttcatt tgtatgagta atactgaccc atactttgcc ttcatgaggt gaacgcgtca
     3241 caacaggctg accgttttca ttttttaaaa tctcgacatc ttgaaaggta acttttccga
     3301 tcccagttcc ccaagcttta gagaaagcct ctttgcaagc gtaacgcccg cctaaaaatt
     3361 ctacttggcg atgaaaaggc aaggactgga atagtttcat ttcatcagag gtcaagattc
     3421 gtgcaatgaa ttttggtttt tcttcgatca gccgagtgat cctaggaagt tcgactgcat
     3481 cgatcccaat tccttttatc atatgctttt ctcttttcaa taaatttaga acactctcat
     3541 tctaccaaat tataaccatt ttgccacaaa taagtccaca aataacaaaa atgaaagttt
     3601 ctcttaaaaa agagaaactt tcattttatg tttaatcgtt atttgaacgg attacaaatc
     3661 cgcgtttttt atcgttgtga cgtttaccgc catctttttg gctgttatta tttttgctat
     3721 aaccgccttt tgatttgttt ccacgataat taccgccgtc acgacgatta cggttgtttc
     3781 cgccaccacg tttgccattt ttattgtatc cttttttctg cattggcaat gggcgttctg
     3841 gtgtgatctt cactggcact gcatcagctg gatcttttgc tgtcgttttt agcaaaagtg
     3901 caactaaatc ttgtgcagaa tattcttcta ataatttatc tgctgtttgc aggtacttat
     3961 ctagaccatt ttctgcaagt tttgtttcga tttgttcaac tgcagcacct aattgacctt
     4021 tgaatgcttc tttttcggtt ggtggacgta aagtcgtcat ccgttttttc gtcaagtttt
     4081 caatcacatg caagtagtcc atttcatttg gtgtaacgaa agtcacagac ataccgcctt
     4141 ttccggcacg gcctgtacgt ccgatacggt gaacgtagct ttctggatct tgagggatat
     4201 catagttgta cacatgagtg acacctgaaa tatctaatcc acgtgcagca acgtctgtcg
     4261 ctacaaggat atctaaatga ccgctcttaa atgaacgcaa tacgctcatt cgtttttgtt
     4321 gagacaaatc gccatggatt ccttctgcac ggtaaccgcg tgcttctaat ccgcgtgcca
     4381 attcgtctac acggcgtttt gtacgtccga agacgattgt tagttcaggt gtttgaacat
     4441 caaacaagcg agtcatgata tcgaattttt cgtattcttt tgcgcggaca tagtattgat
     4501 cgatcaagtc agctgtcatt tctttcgctt tgatcttcac atgatgtgga tttttcatga
     4561 atttaacgcc aatattcttg atagctggcg gcatcgttgc tgagaaaagt aatgtttgtc
     4621 tttgatcagg aacttgtgaa atgatttttt cgatatcttc caagaagccc atattcaaca
     4681 tttcatctgc ttcatccaaa actaatgttt gaacggttcc aagtttcaat gtgtggcggt
     4741 tgatgtggtc aagcatacgt cctggtgtac ccacaacgat atgtggacga tctttcaagc
     4801 cacggatctg acggccaata tctgctccgc cgtaaacggc ttgtacacgt acttttttgt
     4861 cgcgccctaa acgatataat tcttcttgtg tttggatagc taactcacgt gttggtgcga
     4921 taactaaacc ttgtaattcg tgacgatctg gatcgatttt ttctaacatt ggtaatccaa
     4981 atgcagctgt tttccctgta cctgtctgtg cttgtccaat aacgtctttt ccagctaatg
     5041 ctagtggaat cgtttctgat tggataggtg ttgcttcttc aaaaccagcg cgttcgactg
     5101 atttcaacaa ttctggtgat aattctagtt ctttaaattt caaatgaatc ctcctatatg
     5161 gttgttaact gaggatgtag cttttttgct accccgtcga tttgttcggt caaagtgtat
     5221 tgtttctttt tgacagtggc cacgcacgtt tttatccatt cttagcccct gtggcaccac
     5281 cgaaaaatca ctttgcgaag tttaggcatt agaaaagcca aacgggtcgc gttcagcttt
     5341 atcattatta tgacatctgg cttcaacaag ttgtttctat ggcaaaacca gtccaatttc
     5401 tcttcatctt tcggcaatag gttattctaa catagttttt cgttttctgc aagtttacag
     5461 atttttattt catttgctga agctgttcta ccacttcaaa taagcccatg ctgttgctgc
     5521 cttttagcat aatactatct gttgcttcaa gattttgttt tacagcatca atcaatttat
     5581 tcttttcttt ttcaaaatag aacacaggta gttgggggta tttttggttc aattcttctt
     5641 ttagtgcatg catttgttcc ccataaagga aaatcatggc gaatttctca ggatcgatat
     5701 gttcactcat ttgcgcatgc atatttaaag actctttccc taattccagc atatctgcta
     5761 acacagcgat tctgcgtcct tttgttggta gatttttaaa agtatctaat accagtccca
     5821 tggctgttgg atttgcatta tagacatcgc tcaaaatagc tgcaccattt cctgccgtta
     5881 gccattcggt tctgttttga gtgatagaaa catgggcgag cccttggcgt atctcaggta
     5941 tggacagtcc aaagaagcgc ccgatcgtat acgcaatcaa agcattcgtt acattataac
     6001 ttccaggcag cgggatcgta aatgtttctc cttctactac aaaatcggtt tgtgcttttc
     6061 cttttgctgt cacttccgca tgaacatcgc catttccaat accgaatgtt gtgactgttt
     6121 gtactaatgt tgccgttaaa ggtgtcagca acggttcatc tgcgggcaca acgagtaatc
     6181 cgtctggtac aagtccgtca gtgatttcca ttttggcttt agcgattcct tctcgcgacc
     6241 caagattttc tatgtgtgct tcaccgatca ttgtgattgc tgcagctttt ggttgagcca
     6301 taagtgaaag ttccgtgatt tcattggcgt gatccatgcc catttccaag acgatctttt
     6361 ctgtatcttg gggcatgtgc aaaatcgtgt aaggaagacc aatattattg ttgtaatttc
     6421 cttgcgtctt atatgttttg tatttttcag acaagacaga cgcggttaga tctttcgtgg
     6481 tggttttacc attgcttcct gtaattgcaa cgacttctgc acctactttt tccaagtaat
     6541 aggttgccaa ttgctgcatg gctttcaaag gatctgctac aggaataact ggaaaatctt
     6601 ttggagcttg ttcaacaggc aagctccaaa atgaagcaga tgctccgttt gtgatcgctt
     6661 gctggataaa ttcatgtcca tctcgcgtac cagcaagagg aacgaataaa cttccttcgg
     6721 ttatcttccg gctgtcaaat tcgacattgg taatctttgt cagtgcagag cattcgctgc
     6781 ccacggcttg tgcaatttct tgtattgtta gattcattga gatctctcct aaaaaaggtt
     6841 gtgaaaaaag cgtttggcct aaacggcggg ttcgcatgac tctcacttcc aagtaaattt
     6901 ctctattcgt atttagaagc tagaatgaac gcggttacgc aatcactcca gcttcttctt
     6961 tatttctctt tattcatttt tttcaaaaaa agattgacgc tgatggtatc gattcattcc
     7021 taattgaatc agttcttcaa tcaaatcacc gtatttcaag cccatatttt cccataagag
     7081 tgggtacata ctgaactccg taaatcctgg catagagttt aattcattca ggaataattc
     7141 gtttttattt gtcaaaaaga aatcgcaccg gctcaatccg cttccaccta acatcgtgta
     7201 agctaacttc gcgtactctt gcgctttttg ataaacttct tccggcactt cggctggaat
     7261 ctgcatttcg attttattat tgatatattt ggcttcataa tcatagaatg ctacgtcttt
     7321 tacgacttcg ccaggcaaag tcgtccgaac atcttcattt cctaatacag caacttcgat
     7381 ttcgcgcgct tcaattcctt gttcaacgat tgctcgagaa tcatactgat aggctgttgc
     7441 taaagcattt tgcagctctt ctcggttttc tgcctttgta atgccgacac tagaacccat
     7501 attcgcaggt ttgacaaaca tcggataaag caaagaacct tcacattgat caaatacttt
     7561 tttaggattt tctttccatt gattcttaag tactggtaca taaggaactt gcggcacacc
     7621 agcagcttgt aaaatatact tggtcatgat tttatccatg gcacatgcac tggtcaatac
     7681 gcctgcgccg acataaggca tattcaatgt ctctaagaag ccttggatcg ttccatcttc
     7741 cccgtttggc ccgtgtaaaa ctggaaaaac gatggctcct tcttctttga tttctcccgg
     7801 attgatcact tttcctgtaa agccttcctc tgtctgtcca cttgggtccc atgaaagatg
     7861 caagacatct ttgctagcag gtttttctgt tagtaatgga cctttgaccc attgtccttc
     7921 ttttgtaata aatacgagtt gaacttggta ataattataa taaatggcat ttaaaactga
     7981 aaatgcggaa agaatggaaa cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat
     8041 cttcaaaaga ttgtcctcct gtattctaaa tcgttcagtg atttactctc atattttagc
     8101 acaaaacaaa aataaacgaa taacttattt ataaaaaaac gagttggttt ctaaaagaac
     8161 aaacgtcatt tattttttgt cagctagatt atttgtcccg ttttttataa aaagttatga
     8221 taaagaaaag gagggatgat gatggcgcaa acaaatccac gtagccgctt ggcagaaatc
     8281 attcgagtgt tcatcaatta caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa
     8341 caggtaaaaa aagcctttga agaactaggc cccaccttca ttaagatagg acagatgctt
     8401 tctgttcgag aagatttgct ttcttctgcc ttcactcaaa cattcaaaac attacaagat
     8461 agtgtaccta gcgacacatt ttcaacggtc aaaaagacga tcgaaacaga actttctctt
     8521 tcattatcag atatattcga tgatttttcc aaatcgcctt ttgcctctgc ttctatgggg
     8581 caggctcatc gcgccaagct gaagaacggt gactcggtcg tcgttaagat ccaacaccct
     8641 aacatcgccg aagagatccg acttgatctt caactatttg aacgagcgat ccctttgatc
     8701 aaatacattc ctgaaactag cgtagttgat ctaaaagggg tactccaaga agtaaaacgt
     8761 tccttgatca atgaaatgga cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa
     8821 aagaacaatg gctggaaaga gatccgcagc cccaagattt atgacgcttt ttgttcgaaa
     8881 aaagtgatcg tgatggaaga aatgtctggt aaaaacttga accatttaat gaacgccgaa
     8941 aacaaaactg aaacttttat cactggaatt caaaacaagc agctgaaaca agaagttgca
     9001 aagcttttag ttgagaactt tatgaaacag gtatttgatg atggtttttt ccatgctgat
     9061 cctcatccag ggaatctgct attccatgta ctaaaaaaag aagaacagac acaagctagc
     9121 cgaaaaactg aaatagtcca tgaaaaagag ttcggttcct ttgcttttcg agcctccact
     9181 tctacagaag accctgttgc gccatatacc atcaactaca tcgattttgg tatgatgggg
     9241 catctttctg caggacttag gcaaaaactc acacaagccg ttttagcgct gtatacgaaa
     9301 gatgcttatc gaatcgaaaa agcggtcctt cgactttgtc agcaagaagg ctcgtttgat
     9361 gaaagtcggt tccaccaaga actgacaagt tttcttgaac agtattatga ttctcctatt
     9421 gatgaaatca acttgcaaga agtattcaca cacgtcgtga cgatttgtca tcaaaataat
     9481 ctgcagttcg atcgtgatat cacgcttttg ctgaaagcat ttgggacatt agaaggggtg
     9541 atccgcgtcc ttgaccctga agtttccctc atggaagtcg cttcgccttt tgcccaacat
     9601 tattttctca ctcatctgga tgtagaagat acattgaaac aaagcggatt agacttattg
     9661 gaagggatga aggctgcgcc taagatccct caacagctcc accatctatt agaaatgtgg
     9721 acttcgggac aaggaaaagt caatttggaa ttgaaaaagc aagacaaatt attgagcagg
     9781 atcgaaagta tgatcaatcg gcttgttttc ggtatgattt tagctgcatt gatcgtaggg
     9841 tcctccctgc ttgtccaagc agcacctgta gaaaacgcag aggtcgtttc cttattaggg
     9901 atatttacgt atgctattgc cgcattcgtc attatttttt tggcaatcga tgcattgatc
     9961 caaatgtata agaagaggaa aaagtagaaa aaggttgtga cagaaagaat cagcttcaag
    10021 aaataagaag tgattttgca taaattgttt ctcaaatttt tgtgaaaatt cggcttattt
    10081 ttcgaagagg ctacttctga agcaccgaga ataaaaaatg tgaaaaagct gttctgcacc
    10141 cagtaataag aacaatcaaa aaggaacatg gagcacattt atcctgtttc cctgttccct
    10201 ttttgatgta ttcgagcata aataaaatac cttcgttacc tactatcttc tgtacttggt
    10261 aaaccttggt caattgcctt ttttaacgct tgacaaaaag acatatgatc tttctccgta
    10321 aagggttttg gaccctttgt ccttatgcca gcttttctta actttccact atgataacgt
    10381 tgttctagaa gcgtgccgat attcgcttcg tcaaattctt ttccagagtg atggaacact
    10441 cggacgccct tcggcaatac aagtgaagct aaaccataat cttgcgtgat caaaaagtca
    10501 ttcttcttaa ccataccgac gattttataa tccgcactat cagctccgcg gtctacgtag
    10561 acgatttgga catgattagc gtattctttg gtcgtataat ggtccacact ggtcacgata
    10621 accacgtcaa gaccatagga ttctcctaat tttatgacat cttcttttac tggagaacca
    10681 tctccatcaa tgaatagcct cattcttttt cgccttcttc cataaatgat aaacagttac
    10741 taatccgctc agtaaaagac caacattgat ccattttgcc aagataagca gttgtacact
    10801 ttctatcggc actgattcat ggttcagcgt gatcaataat agatggattg gctctcccat
    10861 ttcttttgct aaaacaaaca aaagcgtcat caataaaaac tggtggatca cactcccttt
    10921 attttgagta gtatacttga caacgatccg gcgatccatt ggaatagctg catcagtttg
    10981 ataatattcc ttatttagcc agctgcctgt tactacgtgc cagaaactaa gcagaaagac
    11041 acctatcgca aaaccatttg gaatcaagag gaccaataaa gaagaactca gtccaagcaa
    11101 ttgcatagcc aatggagacc atgttaaacg tttcaaaatc catggtccga ctatttttgc
    11161 tgcaatcaag cctgctgcat aaggcaaata cataaaaatc gaaagttttt ctaatccgta
    11221 cattatgcca acataaaaac tgctaaataa aaaaaggaaa ttccctagca ttccatttag
    11281 aaaagtaaac agatttagcc agcttggcaa cttccattcc ttcttctggt gtcctaaaac
    11341 aaacacgaac aagatgaaca gcagcaagaa accataaata gcaagatcaa aaagcctttc
    11401 atcgaataac aatcgggcat ttctcaaaag aaacaataag ccaaagaata tcaaaaataa
    11461 aacaagctct cgcgtagcta ccagatttct tttgatgtct ttgaaatcca gttcgtaacg
    11521 cggataatga ctaaccgtat ggtaggccat gacgtaaaaa agtgtataaa tagtaaagct
    11581 tgctgcaatc tgagtcggtg caggcaactc aatcgcttga tacaggagaa atagccagat
    11641 agctgcaaat aagtactttt tcccatccat attgataaaa ccttgcgtct tttcgtgaaa
    11701 attgaccgtg gtattagcag gtacaagcca tgctgcactc agccctagca aaacacttga
    11761 aaaataaaat aaaggaaaat aaaatacgcc taatattcca agcaatgaac cagcaccacc
    11821 aatcagcaga gcgatcatca gcaacgtgta actatctaga ccaaatcgta gactgttgac
    11881 taaaaatagt ccagtcatcc gaaaggcata aaacagcgta aatggcaaaa tcgtcagcca
    11941 attatgcgag tctgttagtc ccaaaaataa aagataagga ataaaaataa ccgtgttact
    12001 taagataaag ggcgttgttt gtgtaaaatt acgcccaaat cgtttgagtt tttcgaacat
    12061 gacaatggct ccttaaacag tcttataact aatctgctct tcgattttag cattaaaggg
    12121 gaaataaacc aattttacag aatatattct tgtaaaaaaa atacagaaag aagcataagc
    12181 ttctttctgc atttttacgg tacttgatta ctcaagcaga caagcccttc tccacaaaag
    12241 tcttgcccga cacccctgca tccggcaggt gataaagata gaaatcaatt atcctgtgcc
    12301 tccaaaatca attaggagct ctgtaaacca gagtccttct cgacccgtcg cataaattta
    12361 ctttaacaca gttcctatgt ttatgcgaga gcttttttca ttttataatc gaccatttga
    12421 aatccttgac cgatatctga aataagttct tctgtaatct caaatccttc atgcttataa
    12481 acctcgattg cttgatgatt cccctgattc actcgcaaaa agatctctct ttcctttgtt
    12541 tgcgcagcaa gttcttcata ccagcgaaaa actgaagaag tcaatccttg accacgtact
    12601 ttatttagca aatataattt acttaaataa atttttccat ttatctcttc ataagcagtg
    12661 taaccgacag tcttttcttc gctttttaat aaaaagtagg acacaccttc tgtcagttct
    12721 ttttgaatct gtttttttga ctgataagtt ttcagcatgt aatccacttg tttttgacca
    12781 atgatcggca caaaagcttc cggccatact tcttgtatga ctgccatcag ctcatccagt
    12841 tcattttctg ttacttttat tttttccata cacccacgtc cttttttaca ttataaatga
    12901 ccttgcgctt gatggaataa aaagagcatg atcatgctat acacacccac cgaccacggt
    12961 ttcaagaaaa tcacaatctg cataaattcc ctaaaagata atttcgtcaa accagctact
    13021 agacagatca agtcgtctgg cgcaaaggga agcagcaagg taacaactag tactttttta
    13081 atatctttcg tttttcttgt cagcaattca tcaaatttct gatacttttt tggcgataaa
    13141 ataagctgga caaaggctac gccatagtaa cggacaagca aaaatccgat tgcttcccca
    13201 atcactaggc caatacaact atataaaagc ccaaatccat tgccaaacat cagcatgccc
    13261 gctactgagg agatccctcc cggcaaaata ggaatgatca cttggatcac ttgcaacaga
    13321 acaaatatca tcactgcata cttgccaaat tgctggataa acgcttgcaa agacgtgctc
    13381 gatcgaaaaa tcccatgatg ataagcataa agaatcaaca caaagaaaaa aaacagacca
    13441 ataatgggca tgatctgaag acatttttgt aaaactgatc cttttttaga catctatatt
    13501 ccccacatct tttttcattt attaaaactg cctcctt
//
LOCUS       DACEQH010000070        13067 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.071, whole genome shotgun sequence.
ACCESSION   DACEQH010000070 DACEQH010000000
VERSION     DACEQH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.071"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            149..1072
                     /locus_tag="IW833_002287"
     CDS             149..1072
                     /locus_tag="IW833_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1308377.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1090..2331
                     /locus_tag="IW833_002288"
     CDS             1090..2331
                     /locus_tag="IW833_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1308378.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            2465..3811
                     /gene="gor"
                     /locus_tag="IW833_002289"
     CDS             2465..3811
                     /gene="gor"
                     /locus_tag="IW833_002289"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ1308379.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(4625..5578)
                     /gene="menA"
                     /locus_tag="IW833_002290"
     CDS             complement(4625..5578)
                     /gene="menA"
                     /locus_tag="IW833_002290"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ1308380.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(5591..6694)
                     /locus_tag="IW833_002291"
     CDS             complement(5591..6694)
                     /locus_tag="IW833_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1308381.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(6784..7719)
                     /locus_tag="IW833_002292"
     CDS             complement(6784..7719)
                     /locus_tag="IW833_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ1308382.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            8021..9955
                     /locus_tag="IW833_002293"
     CDS             8021..9955
                     /locus_tag="IW833_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1308383.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            10412..10828
                     /locus_tag="IW833_002294"
     CDS             10412..10828
                     /locus_tag="IW833_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ1308384.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            10842..11408
                     /locus_tag="IW833_002295"
     CDS             10842..11408
                     /locus_tag="IW833_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ1308385.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            11431..12417
                     /locus_tag="IW833_002296"
     CDS             11431..12417
                     /locus_tag="IW833_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1308386.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(12572..>13067)
                     /gene="scm"
                     /locus_tag="IW833_002297"
     CDS             complement(12572..>13067)
                     /gene="scm"
                     /locus_tag="IW833_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1308387.1"
                     /translation="TSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSD
                     SSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSK
                     TSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYK
                     KSKR"
BASE COUNT         4069 a         2370 c         2700 g         3928 t
ORIGIN      
        1 caatctcttt ttatatttgg acagaatata tcgaacttta ttttgatttt ttgtgaaata
       61 attttcaaaa atccgacttt aaatagaaga agtattggaa agtttttaga gctatgctac
      121 tattatgaag ttgtaggagg agaaaattat gatcgaactc ataaccacag tagaaactat
      181 tgaacaagca gaacagttgc ttgctgcagg tgcagataca ctatatttcg gtgaagagca
      241 atttggattg cgtctgcctg cttcatttac acgagaagaa cagcgacaat tggtagaatt
      301 agcacacaag tatggtaaaa aagcgaatat tgctgtaaat gggatcattc atcctgaaaa
      361 aatgaaaaat gtacctgaat atttggcttt tttaaaagaa ataaacgtag atgtgatcac
      421 agttggtgat acaggtatta tttatacgat gaaaaaaaat cctgaattgc gtataccgta
      481 tatttatgat gctgaaacat tagtaacgag cgcacgtcaa atcaatttct gggcgaaaaa
      541 aggcgcagtt ggagcagtac ttgctcgtga agttcctttt gaagagatga aggcgatgga
      601 agaaaaactc gatattcctg tagaaaccct tgtgtatggt gctacatgca tccatcaatc
      661 caaacgtcca ctgctacaga attactataa ctatacgaaa caagacgaac aaaaagatcg
      721 tgaaagaggt ctttttatct cggaacctaa aaaagaagag acacattatt ctatttatga
      781 agatagtcat ggcacacata tttttgccag caatgatctg aatctctcga atgaattgaa
      841 tgaactttat cagcatcatt actgtacatg gaaattggac ggattattta ctccaggcga
      901 aaaattcgta aacatcactc gactattcgc cgaagcaaaa aaacaaattt tagaaggtag
      961 ctgggatcaa gaaaaagcgg aaaaaatgac tagcgaaatc agtaaattgc atcctgaaaa
     1021 ccgtgaatta gatacaggat tcttttatat tgatccttct gctatcaaat aaggagtatg
     1081 atgaaagaaa tgacagtaaa aagaaaattg aaacgaccag aaatcctagc gccagcagga
     1141 acactggaaa agttaaaaac cgcgatccat tatggagcag atgcagtata tattggcggg
     1201 aatgcatatg gtttaagaag ccgtgcaggt aattttactt atgaagaaat ggctgaagga
     1261 gttgcttttg caaaagaaca tgatgcgaag gtatacgttg ctgctaatat ggtcacccat
     1321 gaaggaaatg aagatggagc aggggaattt ttccgcagtt tgcgtgatgt cggtatttct
     1381 gcagtgattg tttctgatcc agctttgatc gaaatttgtg cgacagaagc cccaggtctg
     1441 ccgatccatt tatccactca agcttcagca actaattttg agacattgga attctggaaa
     1501 gaagaagggt tggaacgtgt cgttttagct cgtgaagttt caatggagga agtggcagag
     1561 attcgtaaaa atacggatac cgaaatcgaa gcatttatcc atggtgccat gtgtatctct
     1621 tattctggtc gatgcacatt gtccaaccat atgtcgatgc gggatgcaaa ccgtggcgga
     1681 tgttctcaat cttgtcgctg gaagtatgga ttgttcgata tgccttttgg tcaagaacga
     1741 aacagtttaa ctgatacagg cgaagtggaa gaagaattct cgatgagtgc agtggatatg
     1801 tcaatgatcg aacatattcc agaattgatt gaaaacggcg tagacagctt caagatcgaa
     1861 ggaagaatga aatcgatcca ttatgtatct acggtgtcaa atgtctataa agcggctgta
     1921 gacagttata tggaagatcc tgaaaactat gtctgcaagc aagaatggat cgacgagtta
     1981 tggaaagtag cacaacgaga attagctaca ggtttttact acaatacacc aactgaaaac
     2041 gaacaattat tcggtgaacg tcgtaaaatc ccagtatata aatttgtcgg tgaagtatta
     2101 tcttatgatg aggcaacaca gattgctacg atacgtcaga gaaacttgtt ttctgtagga
     2161 gatgaaatcg aattttatgg tccaggattc agtcatttca gtcaaaccgt tgaagttatg
     2221 cataatgaag aaggcgaatc cattgaccga gcaccaaatc caatgatgat tttaactatg
     2281 ccagtcgcac agccggtgaa acctggagat atgatccgaa aacgtaaata aggaaaaagc
     2341 tgatatatcc ctgttttaag catcttcgca tacaaaattc aagaaaagtg ttacacttgt
     2401 tttctgtaga aaaaggatcg ttaaataaag gttttgattg atctcttcaa taaggaggaa
     2461 caaaatgcaa atatatgatt acatcgtaat tggtggcggc tctggcggta ttgcttcagc
     2521 aaacagagca gggatgcatg gcgcaaaagt tttgctgatt gaagcgaatg aattgggcgg
     2581 aacatgtgtc aacgtcggtt gtgtacctaa aaaagttatg tggcaagcaa gtacgatttt
     2641 ggaaacaatt gaacgagatg cctcaagtta cggtatccaa gcagatttaa aaaatgttga
     2701 tttcgccggt ctagtagaaa gacgagaaaa atatattgat tttctacacg gtgcttatca
     2761 aagaggatta gatagcaatc atgtcgaggc tataaaaggt tacgctactt ttatcgatag
     2821 tcaaacgatc gaagtaaacg gtgaaaccta tcgtgctcct aaaatcttga tcgctactgg
     2881 aggccgtgca agtaaaatga ccattcctgg tggagaatac gcaattgatt caaatggatt
     2941 ttttgaatta acagagttac ctaagagagc aatcgtttta ggtgctggat acattgcggc
     3001 tgaactttca ggtgtatttc gtggtttagg tagtgaagtc atgtgggcat atcgaaaaga
     3061 acgaccactt agaacgtttg ataaaatgct ttcggacaat ttgatccaaa tgtaccaaga
     3121 agctggaata aaaacttacg cacatcatat tgctaaagaa attactaaaa acaatgacga
     3181 atatacggtg ctatttgaaa atggtgaaac cttaactggt gattgtgtac tttttgccgg
     3241 tggacgtgta ccgaataccg aaaaattagg tctggaaaat acaaatgtgg aattggacaa
     3301 aaaagggttt atcaaagtag ataaatacca aaatacaacg gatgaacata tttttgctgt
     3361 tggtgatgtg atcggtaaat tagatctgac tcctgttgca atcgcagctg gtagacgttt
     3421 atcagaacgt ttatttaatg gaaagaaaga ttcttatctt gactataaac ttgttccgac
     3481 agtcgtcttt acgcatccac caattgcaac aattggtttg acagaagaag aagcattgga
     3541 aaaatatggc gaaaatgaat tgaaagttta tcgttcacgc tttaccccaa tgtactttgc
     3601 attaaatgat tatcgccaaa aatgcgaaat gaaattgatc tgtgtcggaa aagaagaaag
     3661 aatcgtcggc ttacatgcta ttggtgttgg tgtcgatgag atgctgcaag gttttgccgt
     3721 ggctatcaag atgggtgcaa ctaaagaaga ctttgacaat acagtagcca tccatccaac
     3781 aggagcggaa gaattcgtaa cgatgcgata atttaatatt aacagttcca acagcttttt
     3841 attgaacaaa ccatgtaaaa ccagaaaatc agtatactga ttttctggtt ttttgttttt
     3901 tagttattta tgtaagattg cttcgataat tctcaaaacg aaataagtag ggaagttgga
     3961 gattttgatt atcttcgtaa ctgacgaaga gtaaatgaat ccatttggta tgcactctta
     4021 agttcaaaat attggtatcg atgtttaaaa gaaatagaaa tgtatcgaag aacagatgtt
     4081 taaaaaagtg tgactttgta taaataaatt agaagaagca aacgaggatg ttaaaagaat
     4141 accatatagt taccaaacca agaaagttct cgtcaagttc gctggtacga atcaaatcaa
     4201 gtgaaaaaag ggactgtgaa aaaaattgtg tcatagttag tcactttttc cgaacaaata
     4261 gtgttcaatc agctgtcctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
     4321 tttccatttg tttctctaat actcaggaca aaacactgat tttacaacct ctgataaacg
     4381 gtgttcaaaa gccagtttct cggtattaag ccaaatttat gcaaaaatgt ggaaagccat
     4441 tttcacaaaa tttttcttaa tactcgaaac taagcgactt tttcacaacc tcctgataaa
     4501 cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tataaggagc
     4561 tatttttatt tggctgttct tattacttga tgcagggcag ttttttcaca acctcttcat
     4621 gctgttaaat ggcttgcata atgattgtta gtaatagccc caatacataa ctggcattga
     4681 ataagaccat atttttgatg gaatagctga aactgatagg ctggggtaac gtttgcttga
     4741 attgatccaa atgcttcttc acaattggca aggtcaagaa ggtgaccaag atcggccatt
     4801 catagatacc gctcaacaac cccattaaaa tcacgaaata gcaagcatac atcaatagct
     4861 ggaataagac tgttcccgca ggacgtccga tataaaaaac tagtgtatag cgatgatttt
     4921 caatatcacg gtcaagatcc cgcaaattat tagccaacat gatattggca atcgtaaaca
     4981 ccaagggtaa ggaagctaat acgattgctg ctatagccca aagatttcct accaaatcaa
     5041 attgtccaga tgaaaagttg actgtcagat catagacatc tgaaccgaat gtattgacat
     5101 aaacgaccat ggcaaaaatt ccaaggccca tagtcacccc actgaaaatt tcccctaaag
     5161 gcattcgaga taaaggaatc ggtccgaatg tatagaatac tccaataaaa caacagataa
     5221 atcccatcaa aagtaaaagc caacccgtat tgatcgttag aataacgccg ataacaaaaa
     5281 caaatgcaat catagctaaa atcatgtttc gaatcaatac aggagataga tttctgcggc
     5341 caatgacatt ttcttcatac ttatatactt cagaatgagc cttttcataa tccatgtaat
     5401 tattgatagc ggtagtcgct aaatcaaaaa ctagcattcc aataaaaaat aaaaaagtgt
     5461 ttagccaatg tatctcatga aaaaaagcga tagagaataa tacccctacc gcaaaaggaa
     5521 atacactagc cacctttgtt cgtatttcaa ctacttctaa aaagattttt agtgtcatgg
     5581 ttcgtactcc ttattttagt tcagaacggt cacccatcgt gtaatgggag tcctccgata
     5641 atttgaaggt gtctttgatt ggatcggtca catatacttt atcgtctttt gtcacgaaga
     5701 tcgcttccgt cccgttattc aattcgcttt cgacgtattc cagtcctttt ttcacaccca
     5761 ttgaaaagac tgctgtagac aacccgtcac catcaattga tttatctgtt atgatcgtga
     5821 cacttgcgat atcattgtcg aacggataac ccgtttttgg atcaaacaaa tgatggtatt
     5881 ttttgccatc tacttctaaa taacgctcgt aaattccaga ggtaactaat gttttgttcc
     5941 gttctttaat agaacctaaa acagatccgc gagccatgtt cggatcttgg atgccgactg
     6001 tccagtcttg gttttcgcct cttgggctgt ggcctaagac atagacatta ccgcccaagt
     6061 caacgatggc agtggtcact ccttgttttt tcaagacttt cactacttcg tccgtaatgt
     6121 aacctttggc gattgcacct aaatcgatga tcattccttt ttcttttaga taaacagttt
     6181 tttctttgtc atttagttcg atcttatgat aatccactaa tttcaatgcc tgatcgattt
     6241 cttcttggga aggttttctg gcgtcatcaa aaccgattcg ccataattgt gtgattgcac
     6301 caatcgccat atcgaatccg ccttgagaat cttggctata ttcgtaggcc cgcttgacta
     6361 acgtgtagac gtcatcagaa acttttactg gtttgatccc agcttgctca ttcacttcat
     6421 cgatctcaga acctttttga ttgatcgtga ttttatcacc aagttctttt actcgatcga
     6481 aggcaggttt caatgctgat tctttgcctt catcatacac acggatacga acgtaagtcc
     6541 ctaacaagaa ctgttcatca gaatatggat ctttcaatat cttggtagct gctgaacttt
     6601 cagtggttgt tttatttgct gagttgctgc agccagttag caccataaaa gagatgccca
     6661 gtgtgattaa ggaaaatagt agttttttcc gcatcttttt cctctcttca ttttgaaagg
     6721 tgagacgact gcctaatctg gctatcgtcc caccctccaa cttttattag tatgattatg
     6781 tgattatttt tctgttacga tattatctac ttcgattgtt tgagtgtcgc ctttttcagc
     6841 tgcattcact aattgttgtg cgtacatgat gaatgaatgt gagctgtgtg tagcacctgt
     6901 aacaacttct acattagcag gtgagccatc ttcttcgccc attgctttga cgaattcgtc
     6961 gttcagagta gggatgtaag tttttggttc tgtaccgctt tttgctttca tattttcatt
     7021 atattctgta tcatcttgtt ttgatttacc gtctttgtcg atataatcaa agtttgattc
     7081 agttactttg ccgccagcaa ctgtcatttc gaattgcaca cggtagccgt tagagtagtt
     7141 cttttcttca agcttgtaag tgccgtcttt caaatcagca ccattgtcga tttcgattgt
     7201 atctgtgtta ccagcttgag cagcttggat caattgttgt gcgtagtttt ggaagctttc
     7261 agatgagtga gtagctccag ttacgacttc cacaccgctt gcgctttgtg cttttaagaa
     7321 ttggtcattc aactcaggaa tgtatttttc tgggtttgtg ccagattttg cttccatgtt
     7381 tttgttgtat tctgcatctt ttgtttttga ttcgccgttt tcattgacgt tatcatattt
     7441 tgattcagtg attttgccgt cttttacaac catttcaaag actgcacggt aaccatttga
     7501 ataatttttt tcttctaatt tatatgtgcc gtcttgcaaa tctccgccag ctacaacttt
     7561 tgcagatgat tctgttgtgc tttttgcagt cgaagattgt gctgtagatg attcagaaga
     7621 agagtttgag cttccgttgt catctgcgcc gcaagcgcct aacactagag ctgaaaatgc
     7681 taatacggca aatccagtta caaaacgttt tttcttcatt ttaatacccc accattccat
     7741 ttctagtcca caatttttct ttgtgattca ttatacattc taatcttttt agcctataag
     7801 tcaactattt ttgataaata acccatgtaa tatttcaaac gttttctttc agcaaatttc
     7861 gtgaaaaaac gctttatttt aaaatttgaa aaaaatccaa aaggagttag acaaaagaac
     7921 agaacatcta tataatatat ggagattgtg taataagtat caaactaaaa gaattatcat
     7981 atattcatta tatagatagt ctataaggag cggatgatca atggcaaagc aaaatattgt
     8041 cgttgtagga gcaggatatg ccggtgtttc agctacaaaa tacttggcaa agaaactgaa
     8101 aaaagaagat gtagcaatta ctctgatcga tcgtcattcg taccatacta tgatgaccga
     8161 gttgcatgaa gtagctggcg gacgtgtaga accagaagca atccaatacg atctgcagca
     8221 tttgtttgct cgtcaaaaga atgtacaact tgtcacagac actgtaatcg gtatcgataa
     8281 agaacgaaaa atcgtcaaga ccaaattagg ttcctatcct tttgatcagc tgatccttgg
     8341 aatgggcgga gagccgaatg actttggtac acctggtgtc aaagcacacg gattcaccct
     8401 ttggtcattt gaagattctg taaggattcg ggaacatatt gagaagacag tagcaaaagc
     8461 tgcattggaa ccagatgatg aactgcgtaa agccatgctg actttcgttg tctgtggttc
     8521 tggatttacc ggtattgaaa tgattgggga actgatcgac tggaaaaatc gcctagccaa
     8581 agattataaa ttagatccaa acgaaatcac actgatggtc gtagaggcaa tgccgacgat
     8641 cttgaatatg ttggatcgaa atgatgctgc aaaagctgaa cgctatttgc gtaagcacaa
     8701 cgtggaatta ttattgaatg caccaatcgt agaagtggct gctgatcata tcaaattgaa
     8761 agacggacgc aacattccga ctcacacact gatttggaca gctggtgtca aaggaacttc
     8821 tgacgctgct gactttggat tagaatcagc acgcggacag cggttgatcg ccaatgaata
     8881 tatgcaggca aaaggctacg aagataaaaa catctatatc attggtgatt tagtctttta
     8941 tgaagaattc ccaggaacac cgacaccgca gatcgttcaa gctgctgaac aaacaggaca
     9001 cacagccgca gcgaatatcg tggcttcgat caaaggcgga gaaaagcata aatttaaagg
     9061 aaactatcaa ggatttatgg tttctattgg tgccaaatgg ggggtagcta acctatttga
     9121 taagatccat ttaagcggat ttttagctat cgtaatgaaa catatcgtga atttgaaata
     9181 cttctttgat attcgttctg gttattatat gttccaatat ttaatgcatg agtttttcca
     9241 tatcaaagat gatcgcaacg tgactcgagg acattcttct cgttacggca atgtcttatg
     9301 gagtgtgcca cttcgtattt tctatggttt agtttggttc gttgaagctt ccaaaaaaat
     9361 cattgggaat ggtgaagtgt tcaaaccaag tacttggttt ggtgaaggat catggttcac
     9421 tggaaacagt gtgttccctt ggacatggga atacaatgta acagatgttg ccagtggtgc
     9481 ttctgctgct ggtgatgcaa ccacagctgc cagcggagca ggggcaagtg ccggagaagc
     9541 agcgactcaa gctgcacatt ttggtttgag ttatgcatac ggtgaagaac caatggctgt
     9601 gattgagaag tcaccagact ggttcacaaa gatcatgcaa gtgatcatgc caaacaatga
     9661 tgtagcaatg ttcttccaaa aattcatggt attctttgaa attgccttgg cattagcatt
     9721 gattgctgga ttattcacat ggctatgcag tgcgacaacg attgtattgg tgatcacgtt
     9781 ctgcttatca ggcatgtttt actgggtaaa catttggttc atttttgttg ctttcgcatt
     9841 gatgaacggt tccggacgtg cgattgggtt ggatcgctgg gtaattccat ggctacaacg
     9901 tacactaggt aattggtggt acggtagacc aagttcccag tacggcaaga aataaataga
     9961 taaagaacgt tatttgaatg caagatttca atagaaaggg gctgagacaa accgtcacag
    10021 cctccttttt atgtaaaatt aaaggaataa gatgcccgag ataagcctcc atgagcaaga
    10081 cacaggtgtc acaacctctg ttacaacctc ttgttataaa cggtgttcca gaagtagctt
    10141 cttcgaaaaa taagccgaaa tttcacaaaa acttgaaaag taattttcgt aaattccttc
    10201 ttatttcttg aagttgccca cttctgtcac aacctcttgt tataaacggt gttccacctg
    10261 ctgcttctcg gtaataagcc caaagaatta gaaaacatgg aaagctgttt tctattcttc
    10321 gttcttatta ctcaaagcaa gacacaggtg tcacaacctc ttgtttttat gctaaaattg
    10381 ggtgagttaa tctggaaaaa ggagaacaaa aatgagcctt aaagaatttg ctgaaaaaag
    10441 tctgctcaaa ccttgggatg tttttattat tttgcttttg actgttcttt cttttttacc
    10501 agtcatgatt tttggtcttc agcaaaacac cgcaacagca gaaaaagaag cggtactgcg
    10561 tgtggatggc acagaaatca aaacattccc tttgatggct ggagaagaga gcaacacata
    10621 tacttacaaa gatgaacacg gagattataa cttgatcgag atagatggcg atcgagtacg
    10681 gataaaagaa gcagattgcg atgaccagat ctgcgtacgt cgaggctggg caacgaaaaa
    10741 tggcgaaacc atcgtctgtt tgccacataa attagtgatc gaagtccgtt caacagatgg
    10801 aggtgacgaa gacagtctga tttattagac cttcacacac catgagtaaa ttacaaaaaa
    10861 tgatttatat ttcgctactt gttgcacaag gagtgatcat tggtttattg gaaaatatga
    10921 tcccttatcc tttttctttt gcgcctggtg caaaactagg cttagccaat ctaatcacga
    10981 tcgttgccat ttttaccatg aaaaaacaag acagcttctt actactttgg ctacgactat
    11041 tcctgactac tttgctaggc ggaacgattt ccacattcct ttatagtatg agtggtgctt
    11101 tgcttagtta ttttggaatg ttattggtca agcaattagg accaaaacgt gtcagtatca
    11161 tcggaattag tgcggccggc ggtttcatgc ataatgtcgg acagcttttg atggcttctt
    11221 tcattgcaca atcatggaca gtcatgttgt acttgccagt cctttcagtt ttagggattt
    11281 tatcagggat tgcaatcggg atcgcagcga attatctttt acgacatgtg cagacacttc
    11341 aacgattcca aaaagattat gagcagggaa ccaaacaaaa atggagccca atgtttacca
    11401 aaaaataatc gtcacacaag gagttttttt atgaagctac atccaatgtg ggaaaactat
    11461 cctgctttaa gtaaagaatt gacagaaaca ctaaaacgga tggaagaaac tgtccgcctg
    11521 aaaaacaaac ctgtcgaaca agcaatcaaa gaaacgatcc atgccggcgg aaagctgttg
    11581 cgtcctgcct atcagctatt gttctcacaa tttggaccag agcaagatcg agacaaagcc
    11641 atcgctttgg cagcttctat cgaaatgctc catacggcca cactgatcca tgatgatatc
    11701 gttgatgaag cggatctgcg acgtggtcag cctaatatcc gtagtcagtt tggtaatgtc
    11761 gtttctgtct atgcaggaga ctatttattt gtctgctgtt ttaaactatt gtctgattac
    11821 tccacttcct tgaaaagtct ccaattgaat tctcgcagta tggaaaaagt attgaatggc
    11881 gaattagggc agatggatga tcgctataat tatgaacttt ctgtaaaaga atatttggaa
    11941 aatatttcag gtaagacagc tgaactgttc caattaagtt gttctgttgg cgcttttgaa
    12001 agtggaacaa gcgagcgctt cgcaaaaaaa gcaggagata tcggactttc tatcgggatg
    12061 gcctttcaga ttatcgatga tattttagat tatacaaaag agagccagga aatcggcaaa
    12121 cctgtactag aagacatgcg tcaaggggtc tattctttac cgttgatcta ctctttgcaa
    12181 aaaaacaaac caaagctact accttattta gagaagaaag cggcaatgac agaagaagat
    12241 gtcgacgccg tacgcaaaat cgttgaacat acaggaggcg tggaagaagc taggaagtta
    12301 gcggcctctt acacagaaaa ggctttgaag gaaataaaga aattacctgc cacctctctt
    12361 cgaacaaaag agaatctctt ttcccttacc caactgattt tggatcgcaa agactgaata
    12421 gggaaaggtt cgatacgtaa ataaatttct cagaaggaaa tctacgtgtt ttttgacaaa
    12481 ttgaattgta gttcttgtat ttatgataaa aagcaaaaat aaagagcaac gtccgttagc
    12541 gacgttgctc ttttttatct tgaatatttt tttacctttt cgatttctta taactcataa
    12601 aagtgattct taaaccgatt acaagtaata taacgcctgc gagtgttatc caattattcg
    12661 attgacttcc agttttagga agtgctcttt ttgatttgtt tccattgtca ttgcttgttt
    12721 tattagttaa gttaaagttc gtcctgttat ccgaacttgt ttttgagaag gagacacttg
    12781 catgacgtgt atcgcttgtg gtagacgacg tgtctgtact atcgcttgaa gttgatgaat
    12841 ctgagctatc acttgtggta gaggatgtat ctgtactgtc acttgaagct gacgtgtccg
    12901 tgctatcact tgtagtggta gatgaatctg agctatcact tgtggtagag gtagtatctg
    12961 tactgtcact tgaagctgac gtgtccgtgc tatcacttgt tgtggtagat gtatccgtac
    13021 tgtcgcttga agctgacgtg tccgtgctat cacttgtggt agaggta
//
LOCUS       DACEQH010000071        12674 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.072, whole genome shotgun sequence.
ACCESSION   DACEQH010000071 DACEQH010000000
VERSION     DACEQH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.072"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(147..1178)
                     /gene="queA"
                     /locus_tag="IW833_002298"
     CDS             complement(147..1178)
                     /gene="queA"
                     /locus_tag="IW833_002298"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ1308388.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKIGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1371..2546
                     /locus_tag="IW833_002299"
     CDS             1371..2546
                     /locus_tag="IW833_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1308389.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESDTPEESKEV"
     gene            2550..3191
                     /locus_tag="IW833_002300"
     CDS             2550..3191
                     /locus_tag="IW833_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1308390.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3188..4105
                     /locus_tag="IW833_002301"
     CDS             3188..4105
                     /locus_tag="IW833_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1308391.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYDQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVNNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4102..4773
                     /locus_tag="IW833_002302"
     CDS             4102..4773
                     /locus_tag="IW833_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1308392.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATDGTSIILAGALPTALMVIVTDWLLGILERRLDPA
                     SRTSR"
     gene            complement(4969..5803)
                     /locus_tag="IW833_002303"
                     /pseudo
     CDS             complement(4969..5803)
                     /locus_tag="IW833_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016619675.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6004..6270)
                     /locus_tag="IW833_002304"
     CDS             complement(6004..6270)
                     /locus_tag="IW833_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1308393.1"
                     /translation="MSNYTVAIKNSATVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6270..6533)
                     /locus_tag="IW833_002305"
     CDS             complement(6270..6533)
                     /locus_tag="IW833_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1308394.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNIDDIDWEAL"
     gene            7008..7193
                     /locus_tag="IW833_002306"
     CDS             7008..7193
                     /locus_tag="IW833_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1308395.1"
                     /translation="MALNYGNKKWSLDEAEISTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7335..9158)
                     /locus_tag="IW833_002307"
     CDS             complement(7335..9158)
                     /locus_tag="IW833_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ1308396.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9755..12460)
                     /locus_tag="IW833_002308"
     CDS             complement(9755..12460)
                     /locus_tag="IW833_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ1308397.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPILQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         3651 a         2637 c         2389 g         3997 t
ORIGIN      
        1 ttcaaggtgt cttttctata tgttttggaa aataaaaaag atgctggtgt catccaccaa
       61 catcaaaaag tatagagctc tttttctttg tcataaaatc tttgtaaagc ttttttgtta
      121 ttcaaaacta gcggcttttc ataaccttat ttcacgaaca tcgcatctcc aaaactaaag
      181 aaacgatagc gctcttcaat cgcatgctgg taagcagata aagtcaattc tcttcctgca
      241 aaggcactaa ctaacatgac taaagtcgat tttggcaaat ggaaattagt tgagaaagct
      301 tctaccactt tgaattcata gccaggcgta atgaaaatat cagtccatcc gctgtctgct
      361 ttgatttctc cattaaattt cgttccaatc gtttctaatg ttcgaattga agttgttccg
      421 acagccacga ttttcccgcc atttttacgt acttggttca atcgctcagc tgcttcttcc
      481 gtcaaacggt agaattcact atgcatatgg tgttcttcaa ttttatctac gctgacaggt
      541 cggaatgtac ctaaccctac atgtagtgtc agatagacta attcaacacc ctttgcttcg
      601 atctcatcaa gtaattcttt cgtaaaatgc aatccagctg ttggcgctgc agcagaacca
      661 ttttctttcg catagacagt ttgatagcga tctgggtctt ccagacgttc tttgatatat
      721 ggaggcaatg gcatttctcc caatgattca agaatctcta gaaaaattcc ttcatactca
      781 aagtcaatga tccgtccgcc gtgttccaat tcttctttca ctaccgcttg caatctgcca
      841 tcaccaaaag agattcttgt cccaactttg gcacgtttcg caggttttat caatgtctcc
      901 cacgtatctc cttgtgtatt cgttagaaga aggacttcta aatgcgcacc agtatctggt
      961 ttttctccat ataaacgagc aggcagaact cgtgtattat tcataactag cgcatcacca
     1021 ggctgtaact cttcaatgat ttcatggaaa tgtttatctt ccatctctcc aattttccga
     1081 tccaaaacaa gtaaacgaga atgatcgcgg tcttttaatg gtgtttgtgc gatcaactct
     1141 tccggcaagt caaaatcaaa atcttctgtt gttaacatat cgtcactcac tttctatcat
     1201 gtgcttcatc attctatcat gtttttcatt ctgttggcaa aatcagcact ttttcacttc
     1261 tattttttgt tttcctaaat aaaaaaacta ccctatttgt attttttcat tgttttaggc
     1321 atactagtaa tagatagttc ttcattgaaa atagaaaaag aggtggcacc atgattgaat
     1381 ttcaaaatgt gtcaaaaatt tataaaggtg gaaaagtcgc tgtagaggac gtcaatcttt
     1441 catttgaaaa gggtgagttc atttgtttta tcggtacgag cggaagcgga aaaacgacga
     1501 cgatgcggat gatcaaccga atgactgatc caacacaagg gaaaattttg attgatggaa
     1561 aatccatcca agaaattgat ccagtggaat tgcgacgtca gatcggctat gttatccaaa
     1621 gtattggatt gatgcctcac atgacgatcc gtgaaaatat cacccttgtc caaaaattgt
     1681 tgaaagtcag tcaagaagaa cggaataaaa cagctgaaaa aatgattgat cttgtggaat
     1741 tgccccgtga gatgttagat cgctacccac atgaattatc tggtgggcag cagcagcgta
     1801 ttggagtcgt tcgtgccctt gcagcgaatc aagatatcat cttgatggac gagccattcg
     1861 gtgcgcttga tcccatcact cgtgattctt tgcaagacct tgtcaaagat ttacaagagc
     1921 gcttgggaaa aacgatcgta tttgtaaccc atgacatgga tgaagcgatt aagctagcaa
     1981 gccgtatcgc catcatgagt gaaggccgtg tgatccaatt tgatacacca gaaaatatct
     2041 tacgtcatcc tgcaaacgat ttcgtagaag agttgattgg agaagatcgc ctactacaag
     2101 ccaagccaaa tgcgacaaga gtcggagaag ttatgctaaa tacagcaatc acgattacac
     2161 cggaaaaatc attacaagaa gcaatcaaac tgatgaggga aaaacgtgta gatacgttgt
     2221 tagttaccga taattcgaat gtattaaaag gatttatcga tgtagaaacg ttgaataaaa
     2281 agcgaggaaa agtatccagt gtcggcgaca tattaaataa acatgttttt tacgtaaaag
     2341 aatccgccta tcttcgtgat accttgcaac gcattttaaa acgcggactg aaatacgtgc
     2401 cggtcgttga tgaacagaac aaagtcgtag gtatcctgac tcgtgcatca ttagtggata
     2461 tcgtctacga tgtgatttgg ggagaagatc ccgcaactac tgatgttgca gagtcagata
     2521 ctcctgaaga atctaaggag gtctgaccta tgcagcagtt ttttcatcag cacggttcag
     2581 aaattctctc aaaaggtttt gaacatcttt atatttcggg acttgctttg atcattggta
     2641 tcatcattgc tgtaccgctt ggtgtcttac tgacacgttt tccaaaagct gccacgattg
     2701 tgattggctt aaccagtgcc ttgcagacaa tcccttctct tgctttattg gcattgatga
     2761 tcccgctatt tggcgtaggt aaattacctg cgattatcgc actctttatt tattcattat
     2821 tgccgatttt acgtaatacg tacattggga tgaaaaatgt cgattggaat tatcgggatg
     2881 tggcaaaagg catggggatg actgagttcc aatccatttt ttccgttgaa ttgcccattg
     2941 ctatgccaac gatcatggct ggtattcgtt tagctgccgt ttacgtgatt gcatgggcta
     3001 cacttgcctc ttatatcggc gctggtggtt taggcgattt gatttttagt ggactgaata
     3061 attaccaacc agatctcatt ttggcaggta cgatccctgt aacgatttta gcactattgg
     3121 ctgattggct tctaggtctc ttggaacacc ggctgacacc gatcgcactt agagaggaga
     3181 atgaagaatg aaacgtatac taaaaatgac catggttctt ttctctcttc ttatacttgc
     3241 gagttgttct ttccctggtc ttgccagcaa tacagatgaa gacaccattt cgatcacagg
     3301 tggaatcact tcagaagcac agatcttagc aagcctagtt gccggaatgg ttgaacatta
     3361 tacagataaa aataccgcaa tcatcaacaa tctggctacc acaactatca accatcaagc
     3421 gatgatgaac ggagatgctt caatttcagc ggctcgctac acaggaacag atttaacgac
     3481 tacattgaac cttcctcctg aaaaagatcc gaaaaaagcg tttgctaccg tgaaagatga
     3541 atttgagaaa agatacgatc aaacgtggtt cccgtcctat ggttttgaaa atacgtatgt
     3601 ctttcttgtt cgaaaagaca ccgcacaaaa ataccattta tctaaagtca gcgatctgaa
     3661 aaatgtagcg gatgaattag tagctggtgt agatacttct tggatcaatc gtaaaggaga
     3721 cggttatgac ggcttccaag aaacttatgg cttctccttc aattcgatat tacctatgca
     3781 gattggactt gtttatgacg cagtcgaagc aggaaaaatg gatattgttc ttggctactc
     3841 tacggatgga cggatcgcca gttatgatct agtcatgttg gaagatgatc gtcaattctt
     3901 cccaccatat gatgctgctc cagttgtaaa caacaaaata ttagctgata cgcctcattt
     3961 aaaagaagcc ttaaataagt taggtcatac catcagtacg gaaaaaatgc aggaattgaa
     4021 ctacgaagca gataataatc tagtggaacc atcagtagta gcacaacgtt tcttacaaga
     4081 aaaccactat tttgaaggga agtgaagcaa ttggataaaa tgaatgtctt tcaacaattt
     4141 ttctattatt tccaggaaaa tggctcctat atctttgcac aatttatccg tcacttcctg
     4201 atttctatct atggtgtcct ctttgcagca gttgtcggga ttccggttgg gatcatgatt
     4261 tctaggagac gtaaattagc aaactgggtg attcgtttgg cgaatatcat ccagacgatc
     4321 ccttctctag ccatgatctc cattttaatc attggattag gcctgggagt caatgtcgtg
     4381 attgttactg tatttttgta ttccctctta ccgatcatca agaatacgta taccgggatg
     4441 atccaagtag acaaaaacat ccttgatgtc ggcaaaggca tggggatgac tgctagacaa
     4501 cgactattca tggtagagtt accactctct gtttctgtta tcatggcagg cataagaaac
     4561 gcgttagttg ttgccatcgg gataaccgcg atcggtgcat tcgtcggtgc tggtggtctt
     4621 ggagatatca ttatccgagg aaccaatgca acagatggca catcgattat tttagcaggt
     4681 gcattgccaa ctgcattaat ggtgattgtc acggactggc ttttaggtat tttggaaaga
     4741 cggctagatc cagccagccg tacatcacgc tgatgttatt gtaaaaaaca aaagcttctg
     4801 cgaccatttg ttggtcgcaa aggctttttt agttctgtca ttttttaagg caataatcca
     4861 ctctgattct tccctcctta tgggcaaaga gttgcttttc ttagctattt atccattccg
     4921 tatactccaa cttcatactg ccaacgggtt catctctgca tccaccgact aagttttttt
     4981 cttatccgct aaggcatttt gccaatccat tttcccatca ctcgtcatgg aattcatacc
     5041 acatgatctc taaatactcg tctgcttgtt ctttgaattg ggcatcaatt cttacatcct
     5101 ttggcgtggc tttcatttaa gccttttcca tcagtcctcg attgttcgat gccttcagct
     5161 acatcatcaa aactggtgac tgccgtatcg gtaacttcga tcatctttta tacaatcggg
     5221 aacttgccat cctcatcctt ggctaaaaat atatctagcc attccagctt ctcgccatgg
     5281 gaggtttgcg gttcctaaaa ccggtacatg attcttatgg actgcatccg tcgcaactag
     5341 acttggcggt acgatgatcc ttcaccagaa gagcaccccc gtaaaccaat tgatcgatat
     5401 attgccaata agaaaagaca ttggcgtaaa agatatcgat ccctcgtgat gcattgccgt
     5461 tgatactgct attcatggcc gaaagcgcca cgactttgac ttcttccgtt tgagtgacat
     5521 tcatgttcgg caatgtcgct ttggataccc gctctgctaa agaaacgaca ctggtattgt
     5581 atttcgtatc tgtatctttt ccaaaagacc atgccaacga atcctctggg aaccaataag
     5641 acgactccgg ttgattgtcc attgcttttt tcaacatact tttggctgtt tctcgataaa
     5701 cgacatttcc aattgtcttc gcctcacttt ggcgataatc gatggtcgtt gccactgcca
     5761 acactaacaa cgatgctcca attttactga ttttccttgt cacacttctt cctccttgtt
     5821 ttacataaac ccgtcaacat tccattccta aactcaaaca atgacccttc aaaggtggag
     5881 atataggaac aaattaaaga aatcatgcaa taaatccaag aaggttggtt ttagaacaaa
     5941 aaaagagtct cctttttggg gagactcttt gttaactatc taaaaaggtt ggctcttctc
     6001 acttcactca taatgtgtcc aggcagaata aatttccaca actttatttt cctcatcaac
     6061 tttgtaaacc actcgatgct gtctattcag cctgcgtgag tatctgcctt catgtgtagg
     6121 tctcagcttc tcaaaagact gcgtgggcat atatggatct tccttcaatg tctgaatcac
     6181 ttcttcaaat tgtttcttta aattcgattg cttgattttt cttaaatcca ctgtcgcaga
     6241 gtttttgatt gcgactgtat agttactcat caaagagctt cccaatcaat atcatcgata
     6301 ttcgtaaagc cactgtcgtc tttttcgcgg tctcgtactt tatctaaagt acctgtttgc
     6361 tcaagaaaaa gggtttcttg aatcgctctc cagtcttcta gcccaatcaa tacagcattg
     6421 tttccactac gatcactaat aatctcgatt tcagtatgat tttcatttac ctctttcaat
     6481 aactgataaa aatttttacg agctgtgcta ggattcatcg ttgtattagc cattgtctct
     6541 cctccttttt ctgatataat tataacgtac ttgattaagt acgtcaaaaa atacaataaa
     6601 gcacttaaat tcagcaaact tttcctcttt tttacataga gaaaaagacc cctttttagg
     6661 gaggatcttt gtccacgctc acttcttact cctttacgac tagcggtagc caaacgtaaa
     6721 gtaattggta acaacgtatc atctttagca aaggtcgaat ccaaagcctg tgagtctatg
     6781 caacaacacc aatacacgaa ggttcctcgt tgcagtgtga cttgattgat atcttgaata
     6841 acctgaggat acccacgaaa gatttgtaac ggttattcaa aactgatttt ggccgtcaag
     6901 tcttctactg cctcaaaaat gattgcctct ttcgtctcct gccattcatg ttcactatcg
     6961 atttgatact tgcttagacc gtcgggtttg tgaatcacca attaaaaatg gcactgaatt
     7021 atggtaacaa aaaatggtca cttgacgagg ctgagatttc tacttatgaa gacacatggc
     7081 acttaagagt cgaggtaaag gaagaagctg aaaatatagg aggctgtaca ttatttggca
     7141 aaggattcgg aaattatgat caagacatca aaatcaaaat gttttacagc taaataggaa
     7201 gaataataac atttcatacg ctgttcccat ctttaatggc ttatagaggg cacctttagc
     7261 tgggaataag acaaaacaga ccctctagct tcaattaaga gctagagggt ctgtttttga
     7321 ttttaaaact tggtttattt cttcaaatgg tagctatagg tgctgacaac gatattttca
     7381 cgccaagaca atcgcaatct taaccgcaaa cttgtttggt tatggagaat attctgccag
     7441 cgtctgtttg gtacaaattg aggaatcaac acagtagttg tatagttttg tttcgctgca
     7501 tttttactta cgatatctac ataacggata attggatttt caatcgagcg ataagaagag
     7561 tggacgattg aaaaacggac atctgggaaa tgctttttaa attctgcttc gatttctttt
     7621 tctttttcga catcttcgtc tagcgaaaca tgcatcgcaa cgacataatc accgatcgaa
     7681 cgtgcgtaat tcaacgcacc gatatttacc cgtgtcacat tcccgactaa tacaagaacg
     7741 gtattcccgt cataatcatg tagttttgca tcattttcta aacgaagctg ttctgctact
     7801 tttttataat gatcatggat cttataaaaa atgaacataa cgattggcat aatgatgaag
     7861 aaaggccaaa tgtcccctaa ccgatagacg aacaaaatcg caatgattgc atatgagatg
     7921 aacgcacctg taatattggc aatcgacttg cttaaccatc tttttccttc ttttttccat
     7981 ttgacgacca tccctgtttg agataaagca aatggaatga acacgccgat agagtataac
     8041 ggaatcaagc gttctgttga gccatggaaa ataaacagca atacgatcga acctagcgct
     8101 agtgtgataa tcccattgga atagcctagt cgatccccac gatcttgata catgtgaggc
     8161 atgaatttat cttttgccag attgtatgca agaactggaa aagcagaaaa accggtgtta
     8221 gcagcaactg ctaaaatcag cgctgtagca aattgaagga cgtaatacaa aataccatgt
     8281 ccaaagacag ctttaccaat ttgagacaaa acagtgactt ctttttcagg aacgatccca
     8341 taccaatagt tgataaatgt gatccccacg aagaagaaac ctaagatcaa tgccatcatc
     8401 gtcaatgtag cagcggcatt ttttgctctt ggcttcttaa agaatggtac agcattacta
     8461 atcgcctcca ccccagttaa agaggaagaa ccagaagaaa atgctcgtaa aatcaaagcg
     8521 atagaaacac ccggcacagc cgctcctgga agtgcagtag cttgcaatgg cacagcacct
     8581 gtcacgatat tatataaacc aacaacgatc agtaacgaga tcacaaggat aaatgtgtac
     8641 accggaaata ataggaacga tgcagattcc ctcagcccac gaagattcag catcatcagt
     8701 agcaaaacga ttgtcacaga aatagcgact tgatgaccgt agagtgctgg caccgctgat
     8761 gtgatcgctt cagcacctgc tgagacagat actgctactg tcaacatgta gtcgatcaat
     8821 aaagagccac cggaaatcaa gccagcattc tttccaagat tctcactgct gacgacatat
     8881 gctcccccgc catgaggata agcatggatg atttgacgat atgatagcgt taatgaaatc
     8941 aataaaataa taacaaaagc ggcaatgggt agcgaatacc agatggctgc agcagacaaa
     9001 gcgactaaaa cgacaacgat ttgttccgta ccatatgcaa tagatgataa cgcatcagac
     9061 gacaataatg ccaaagcagc aaatcgggtt aatttgtgct catcattttc agcagatttt
     9121 aaaggcttac ctactaacaa cctttttaag taatccaatt tctaaactcc tttacacaca
     9181 attttacttc aagttttata gtcgaaaatt tatagtaaac aagatgttat agtacgctca
     9241 ctgaccttaa aagtcaataa actttaagaa aataattgga aaaaaacaga aaaatatcaa
     9301 gcgctttttc aaacgcttta atacagcaaa aatctaacat ttttttatgg gctttttctt
     9361 tgaattctat aataaaagga tagaacttga cttagaaaat caagttctat cctttttgat
     9421 atcttaactt ttgaaagaga gatgtaaggt tgctttctac ccatcattct tactactcga
     9481 agcatagcgc agttgtcaca acctcttgat aaacggtgtt taaaagcttg catctgcggt
     9541 aataagccca accaaattaa aaacataaga agctgttttt atttggtcgt tcttattact
     9601 tgatgcagaa cagccttttc acaacctctt ggtacacggt gttcaatcag ctgaccctcg
     9661 gcattagctc aaaaaacggc aaaatatgag gaactatttt tccatttttt tctctaatac
     9721 tcaggtcaaa acgctgattt cacaacctct ttttttaatg aaaaatatgg ctctcatgcg
     9781 ccgcttcttt gaatttcgtc attttttctt cgatgaacgg atagattgcc gttcctacga
     9841 ttccaaagac aacaaacaga ccgatcatga tccagattgc tttcgtagca tttggccagt
     9901 aaatgcctcc tgcagattcc cgtaacaagt ttaccgcatg agtaaatggc aagaacggat
     9961 tgatcatttg gaagaatttc cctgagactt ggatcggata gttcccgcct ccgccagaga
    10021 tcgacagtac taagatgata atagcgatcc ctttaccgac attatcgaat aatgcagcca
    10081 agacatagac gatcatcatg aacgctaagg cgatcaagac agcaaatgac acgctgtaga
    10141 ccggattttg gacatctact cctagtaaga aaatattccc taatgtaacg atcaagcttt
    10201 gcgcaatcgc cattgtcaaa aatgtcagca tacgagcaac gaattgttct cgtttcgtaa
    10261 aattgtttcg gtctttcttt cctaaatagt attctgtcgt agcaacactt gaaagaagaa
    10321 cagcgccgac ccacagacat agtgcagtat aaaacggggt gctagctgaa ccgttatttg
    10381 cgattggata aagcgcattg gtttgtagct caacaggttg tgtaaagaag tcactttctt
    10441 ttgttgcatc tagtttcaat aatttgatga cttcgcctag atcgacttct tgttcgcctt
    10501 ttcggatcgc gtctgctgct ttttggatac cagctttgat atttggccag tcattgttga
    10561 tcaagtccgc agcagcgttc acagctgttt cgacttctgg cattttttca tttactgttg
    10621 tcaatgtacc ggtgatttca ttttttagtg ctggccaatc attttggata aaatcagcgg
    10681 ccaatcctaa tttttgctga aggatcggta attcattgtt gtataggtct gccccttcat
    10741 tgatgccatt gacgatggta tccatatttc cattcagcat tgtattggca tcatggattt
    10801 cttgtccaat tgcaggcatt tctttttgat atttttctaa aattgccact gcattcgtta
    10861 cagttgcttt cgttgaactg agaagcgaat caaagtcgat ctggtccgct ttgcttagcg
    10921 ccccttgcgc agtcccaatc gtatcgatga cttggtctaa tagagaagtg acagtggaac
    10981 tgatttgacc tgtattgata ccagcaatga tgccattgat attattaaca acggcatcaa
    11041 tttgatctag caattcatca atagccttcg tgtcttcatt ttgaacagct gtgttcaaag
    11101 cagataggcg gttttgcaga ttgcttagca agggttttaa ttgatttagc tgatcaataa
    11161 ttggctgcaa tttcgtattt ccagcacttg tttgcaattt agtcaggaac tcgaccagtt
    11221 tatcgattgc attttgttgt tgagatattc cgtcaataaa gccgttcaat acattgctga
    11281 tgtttttctt ttcatcatca gtcagcttac catcttcaat agctgttctt acagtatcta
    11341 ttaacgaacc tgtcgttgtt gccacttgtc caattgattt caatgtaaca tctacactgt
    11401 ttgtgatgct tggtatcgca tctttcagct gagaggcacc tgattttgtt tggtttgcta
    11461 gatcacttgc ctgattttct aaatttttca catctggcaa gatatcctgt acttgttgga
    11521 tgatcgtcaa gccttgtttt gcttcatcga ttccttggtt cattgtctct tggatcgaag
    11581 caaaatcgcc gtcgatctct gctagttgtt taccggcatt ttggatctct gggatttttt
    11641 cttgcaaatc aaggatgatt tttgcttgat ctttcaactc aggcattttg tcattcaatg
    11701 caacgacttt ttcacccatc tcatctactt tagggatata atccacgaat tcatttgctt
    11761 tattcagttt ttccttgatt tcaggtagtt gactttgtaa ttcgaggact tgttttgtgt
    11821 agccgtcgat cgtgtccaaa ttttcatctg ttgataaaat catatctttg actttattga
    11881 tacttactag gttcgtatcg atgtcgtaac ctatctcatt gaatactttc aataaagtcg
    11941 aactggctgt tttgataaat tcatttgtga tttgcgattg gatcgaactt gctcctttat
    12001 cggtgatttt cggtgcaatc gcattgattt tttcatttac cgtatattcg atttttggtt
    12061 tcttgatatc accactagta aaactcagca gatcttctga gaaatccttt ggcaagtaaa
    12121 tacctgcgta atactttccg gaacggactc cgtcttctaa ttgttctttt gaatcaacga
    12181 attgccatcc taattgttta tttttatgaa gcgattcgat gacttgctcc ccaatggcca
    12241 cttctttacc ttgaaactct gctggcttgt ctgcactgta aacagcaatt ggcagctctc
    12301 cagtatttcc atatggatcc cataatgctt tgatgttaaa ccacgcatat agcgaaggga
    12361 tgatcatgat cgcgatgatc agaagtgttg caatcgggtt tttgaaaatc cgtttccagt
    12421 caagcaaata taatcgaagc gtatttttga ttgactgcat agttctcccc cttcttcttt
    12481 tttcagctga attactttac catgtatttc atactttttt caatcggctt ttttcaaaaa
    12541 agtcgaattt ttggacatat cacggcaatt gtctcattgt aatctcttac atattgagaa
    12601 ataattaagg attacagact ttactttatc atgaaataaa agggaaagat aattttatgc
    12661 caaagttttt atct
//
LOCUS       DACEQH010000072        12388 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.073, whole genome shotgun sequence.
ACCESSION   DACEQH010000072 DACEQH010000000
VERSION     DACEQH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.073"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(180..737)
                     /gene="frr"
                     /locus_tag="IW833_002309"
     CDS             complement(180..737)
                     /gene="frr"
                     /locus_tag="IW833_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ1308398.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(740..1462)
                     /locus_tag="IW833_002310"
     CDS             complement(740..1462)
                     /locus_tag="IW833_002310"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ1308399.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1598..2479)
                     /locus_tag="IW833_002311"
     CDS             complement(1598..2479)
                     /locus_tag="IW833_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ1308400.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="IW833_002312"
     CDS             complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="IW833_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ1308401.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3719..4198
                     /locus_tag="IW833_002313"
     CDS             3719..4198
                     /locus_tag="IW833_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ1308402.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4415..5506
                     /gene="phnW"
                     /locus_tag="IW833_002314"
     CDS             4415..5506
                     /gene="phnW"
                     /locus_tag="IW833_002314"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ1308403.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5499..5627
                     /locus_tag="IW833_002315"
     CDS             5499..5627
                     /locus_tag="IW833_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1308404.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5631..6176
                     /locus_tag="IW833_002316"
     CDS             5631..6176
                     /locus_tag="IW833_002316"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1308405.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6629..8320)
                     /locus_tag="IW833_002317"
     CDS             complement(6629..8320)
                     /locus_tag="IW833_002317"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ1308406.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8740..9687)
                     /gene="arcC"
                     /locus_tag="IW833_002318"
     CDS             complement(8740..9687)
                     /gene="arcC"
                     /locus_tag="IW833_002318"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ1308407.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9802..10821)
                     /gene="argF"
                     /locus_tag="IW833_002319"
     CDS             complement(9802..10821)
                     /gene="argF"
                     /locus_tag="IW833_002319"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ1308408.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10912..12141)
                     /gene="arcA"
                     /locus_tag="IW833_002320"
     CDS             complement(10912..12141)
                     /gene="arcA"
                     /locus_tag="IW833_002320"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ1308409.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3413 a         2549 c         2215 g         4211 t
ORIGIN      
        1 acttaaaaga ttcccactat cttaagtatt atagcaatat ctaactgttt ttcctatgaa
       61 atcattgttt ttttatttaa aaactgaata attttttctt tttttaattt ttaaataaaa
      121 gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat
      181 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttgatgctg
      241 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      301 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      361 atttttgcat tttctgcttc ttttttcaca tctttagcta attcttttct gcgttcttct
      421 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      481 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      541 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      601 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      661 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      721 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      781 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      841 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      901 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      961 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgcgcgta
     1021 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
     1081 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
     1141 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1201 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1261 tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccgcccaca acgattgcca
     1321 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1381 gtttgattcc aaatcctgcg tccccagcta aagcttctcc actcaatttt aagactacac
     1441 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1501 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1561 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1621 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1681 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1741 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1801 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1861 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1921 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1981 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     2041 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     2101 cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     2161 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2221 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2281 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2341 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2401 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2461 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2521 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2581 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2641 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2701 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2761 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2821 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2881 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2941 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     3001 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     3061 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     3121 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3181 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3241 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3301 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3361 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3421 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3481 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3541 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3601 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3661 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3721 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3781 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3841 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3901 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3961 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     4021 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     4081 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     4141 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4201 agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattga
     4261 ataaaccttt acactcttta caaaaataca tcatttttta ttcaaatttt tcaacacctt
     4321 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4381 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa cttctggacc
     4441 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4501 agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc
     4561 ggaaggaata tacacaacgg tcttaatgca aggtagcagc tccttcgggg tagaatcggt
     4621 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatag
     4681 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4741 ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccgtctat
     4801 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4861 gattgctgct gtcacacaag aaagagatct tttgtttata gtggacgcga tgtcaagctt
     4921 tggtgggata ccgatttgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4981 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactgga
     5041 aaaacgaaaa aatgttagcc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     5101 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     5161 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5221 cgccaataat catgtgctac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5281 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5341 taatcatttt tatcagtatt taaagcaaaa tggatttgtg atctatcccg ggaaaatcag
     5401 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5461 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg
     5521 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5581 cttttctaga aaaaggaacc caactgactg acaaagaaat ccgctaaccg atgggaaaat
     5641 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5701 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5761 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5821 tcgaacggtt gagaaacgct gggatcaata ttggttccac gaatggattt aatgctccta
     5881 tgatgacaat cgtagcagaa accgcaaaaa aagaaaggta ttcaccagac ttcatggcaa
     5941 cttttgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     6001 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     6061 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     6121 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaagatc gtttaaaaca
     6181 gtctgttgct cttagattcc aacaagccgg cgctgatatt tgtgtaaaca atttaaaaga
     6241 gttaacagaa tacattttgt acgctgacaa gaatccagaa tgacttttat acaaaataag
     6301 aggttgtgaa aaagctgtcc tgtatcaagt aataaaaaca gccaagcaaa aatagctcct
     6361 catatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt
     6421 cgaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
     6481 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
     6541 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
     6601 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
     6661 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
     6721 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
     6781 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
     6841 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
     6901 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
     6961 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
     7021 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
     7081 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
     7141 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
     7201 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
     7261 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
     7321 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
     7381 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
     7441 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
     7501 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
     7561 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
     7621 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
     7681 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
     7741 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
     7801 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
     7861 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
     7921 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
     7981 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact actgtttgca
     8041 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
     8101 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
     8161 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
     8221 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
     8281 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
     8341 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
     8401 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
     8461 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
     8521 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
     8581 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg
     8641 ctagtcgttt cataaaaaaa aaatccagat ggcctaaagg agaagaaata ccacctggat
     8701 agctataaat catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt
     8761 tccttcttca gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc
     8821 gtttggtttt gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg
     8881 agcgaattgc ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt
     8941 cttttgatct ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag
     9001 caaatccgca tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc
     9061 ttctcgtcct tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga
     9121 gactgtgata actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat
     9181 gggtttaggg gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac
     9241 agtggagtca gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg
     9301 tttacttgga tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga
     9361 aaccacatct ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca
     9421 gtaaccgata gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt
     9481 ctcagaatca gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg
     9541 ggagatgatc aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat
     9601 taacgcttct tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa
     9661 agcgacaact acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt
     9721 tctagataac aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc
     9781 gtgagatgaa tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc
     9841 catgatcgct ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt
     9901 gctgcggaag acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg
     9961 ttcaccgtaa acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg
    10021 atctgtgttg tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt
    10081 gatacgttct tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac
    10141 gtctgctccg tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt
    10201 ttcagcaaag ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc
    10261 aacgatacgc atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt
    10321 gtttcgacca tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg
    10381 gatagttaaa aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca
    10441 aactggaaca ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa
    10501 ttcgattccg tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt
    10561 acctaattgg atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc
    10621 agtagtaaat gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt
    10681 tccttctaga tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa
    10741 atcgatcaaa tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc
    10801 ttggaatact gattctttca tattaaacat tctcctttag attgttttta tttaagatta
    10861 aagaggaaat cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa
    10921 gtcttcacgg acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc
    10981 acttgaattg atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata
    11041 atttcggtta taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt
    11101 ccattgttct cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc
    11161 atctaatcca agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat
    11221 atcatcacca tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc
    11281 tggatggata gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat
    11341 tgcacggttg ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa
    11401 taggtggcgt gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc
    11461 tactacttta tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg
    11521 atccaaccat acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc
    11581 tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt
    11641 tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa
    11701 tggatagtca tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt
    11761 tgtacgaacc ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa
    11821 ctcatgaaga ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt
    11881 caagaattgt tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta
    11941 aagagtctcg acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc
    12001 aatcggaaga tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt
    12061 ttccacttct tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa
    12121 aacgtgaata ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat
    12181 atcctccttt tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca
    12241 ttaatattcg ataattaaat aattaaacgt ttttctattt ttaagcgtaa tttattcatt
    12301 ttttattctg tatacacttt caaaaaaata tttattcatt gaaaaaattt ttttgaataa
    12361 attaaaaaaa taaattatat tatcgttg
//
LOCUS       DACEQH010000073        11984 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.074, whole genome shotgun sequence.
ACCESSION   DACEQH010000073 DACEQH010000000
VERSION     DACEQH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.074"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            401..625
                     /locus_tag="IW833_002321"
     CDS             401..625
                     /locus_tag="IW833_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308410.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            643..1071
                     /locus_tag="IW833_002322"
     CDS             643..1071
                     /locus_tag="IW833_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308411.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            1049..2467
                     /gene="terL"
                     /locus_tag="IW833_002323"
     CDS             1049..2467
                     /gene="terL"
                     /locus_tag="IW833_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HAQ1308412.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            2479..4014
                     /locus_tag="IW833_002324"
     CDS             2479..4014
                     /locus_tag="IW833_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1308413.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            4094..4972
                     /locus_tag="IW833_002325"
     CDS             4094..4972
                     /locus_tag="IW833_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ1308414.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            4974..5291
                     /locus_tag="IW833_002326"
     CDS             4974..5291
                     /locus_tag="IW833_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308415.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            5340..6041
                     /locus_tag="IW833_002327"
     CDS             5340..6041
                     /locus_tag="IW833_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ1308416.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMIMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            6056..6946
                     /locus_tag="IW833_002328"
     CDS             6056..6946
                     /locus_tag="IW833_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAQ1308417.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            6969..7184
                     /locus_tag="IW833_002329"
     CDS             6969..7184
                     /locus_tag="IW833_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308418.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            7196..7537
                     /locus_tag="IW833_002330"
     CDS             7196..7537
                     /locus_tag="IW833_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308419.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            7537..7908
                     /locus_tag="IW833_002331"
     CDS             7537..7908
                     /locus_tag="IW833_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308420.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            7901..8296
                     /locus_tag="IW833_002332"
     CDS             7901..8296
                     /locus_tag="IW833_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ1308421.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            8298..8675
                     /locus_tag="IW833_002333"
     CDS             8298..8675
                     /locus_tag="IW833_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308422.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            8676..9284
                     /locus_tag="IW833_002334"
     CDS             8676..9284
                     /locus_tag="IW833_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1308423.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            9284..9625
                     /locus_tag="IW833_002335"
     CDS             9284..9625
                     /locus_tag="IW833_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308424.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            9867..>11984
                     /locus_tag="IW833_002336"
     CDS             9867..>11984
                     /locus_tag="IW833_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAQ1308425.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIK"
BASE COUNT         4262 a         1727 c         2531 g         3464 t
ORIGIN      
        1 gacagcaacg gaaatattaa gaataaggat gtgaatttta actccttcta aattgttctt
       61 attatctatc atccgttgct gtctattgtt acatacagat cgcttaggcg gtctttttat
      121 tttggccgaa aacctacatt ttcgatggcc gattattgga ggaggaatag catgttcaaa
      181 ctatctgaaa tcattaagaa gtccgacgtt gagaaattag agatgttaaa aaagaagttg
      241 aagaaatagc agtctcttcg tgaggctgtt ttattttgct cacaaaaata gaccactatc
      301 gggtaatagt ggtcaggaat taaatgaaaa agatgttagg gttgttagct aagtataaca
      361 tcataacgct tacaaaacaa tgcaagaagg ataaattatt atgagaactt actggtatgt
      421 gtcattaaac aataaatatc cgaagccaat gaaaggacag cataggcgtg tagtcatgtc
      481 tgttcaaatg aaggcgaagt attcgattgt agaaatgatc agagaggcaa cgccagtaga
      541 gattgattat tgcaagctag tctattgtgg gtgcggccgt tggaaagagg atcatgtaca
      601 aaaaaatatc agcaagtaca tatagagagg cggtgattaa ggatggaaaa aagcgatacg
      661 aaagcgaaca gatacaaacc tactgctgca gagaaaaaac tcttggaagt attgataaat
      721 ccagataact taggcaaacc ggtgcaagag ttgtgttcac tggcgaaaat aggtaggacc
      781 aaatattacg acgccatgag caaagatggc tttgttgatc tagttaacga aacaacaatg
      841 gatctgatca aaggaaaagc ttctgatgtc cttaatgcca cttacaagta tgcattgact
      901 gaaaaaggtc atcaggatag gaaaatgatt ctgactattg ctggaatcta tgcagacaaa
      961 caagaactca aacattctgg cggcgtcgat atccgaaaac agtatgagaa aatgtccgat
     1021 gaagaattag aagagttggt gaaacggtat gaaaaaatca acgactctta atcgtgttga
     1081 gtatgccgtt gttcaaagag agctagccat tcgaaaagct aggaaaagtt tttggctatt
     1141 ctgccagctt ctttatccag agttttacaa agatgatcgg ttgtatttga aagatttatg
     1201 tcatacgcta caagattttt acgaagataa aatcgacaaa aaaattttgg ttattaacat
     1261 gccgcctcga catgggaaga cttttacagc gcggctattc gttttatgga tgtttggaca
     1321 aaatccaaag acgaaaatta ttaccggatc ttataaccaa atcctgtcat ctttatttgc
     1381 gcaacaaaca cgtgatggaa tcatggttga aaacgaaaat gttaaggctg aatactttaa
     1441 agatattttt ccagaggtgc aaatcaaata tggagatgct gcaaagggtt tctggtcttt
     1501 agatggttca gaagaaaaga attatttagc gacttctcct ggtggaacat ccactggtat
     1561 tggcgcgaat tatgtaattg ttgatgacat cattaaaacc gcagaagaag cctataacga
     1621 gcgtgtttta gattctcact gggaatggta taacaacacg ttggcccaac gaatggaacg
     1681 gccacgtaaa cagatattga ttatgacacg ttggtcaagt aatgatttag ccggaaaaat
     1741 gttgacgaga cgaaaaaata atgttcatca gatctgttat aaagcagtac aagaagacgg
     1801 ctcgatgttg tgcaacgaga ttatgaccca cgatgagtac ttagatgtgg tacaagaaat
     1861 gaatgtagat attgcagaag ccaactatca gcaagaacca attgaccaaa aaggcaggct
     1921 atatcagaag tttcttactt atgatacgtt gcctgataac attatcaaaa tatggaacta
     1981 caccgacacc gccgacaagg gtgccgatta ctttgcgtct cctgtttttg ctgagacgtc
     2041 tgatcatgat gcttatatta tcgatgtcat gtatacaaaa gagccgatgg aaacaactga
     2101 aatagcccac gctaatatga ttattcgcaa tcgagttaat aaggtacgga ttgaaggaaa
     2161 caatggcggg cgtggattca gaagaaattc agaaagaatt acaaaagagc gaggctatcg
     2221 aggtgcagta tttgaagact ttcatcaatc ggataataaa ctgtctagaa tactggcgaa
     2281 tagtgcttgg gtggaaaatc atgtttacta tccagatgat tgggcgacac gttggccgga
     2341 cttttatgag gctatggtta gttatcaacg tgaaggtaag aacgctcatg atgatgctcc
     2401 ggatgcagtt acaggaattg cagaaacgat tatagaagca gatgacttcg agtttattgc
     2461 ttattaggag gtgggtcttt gttttattct ggaatcacat tgaaaaaata caaacgttta
     2521 agaacgaaat attcaacgca aatcgaagaa ggattattcg atcctaacgc ttttatggaa
     2581 gatatgaaac cctttttcga tgatcgagaa agaaagtatc ttgcttatac tagcgagaaa
     2641 aacgagatcg acagtcgtga gaaaccaaaa acaaaaatca ttaaggtcaa taacaagatt
     2701 catgcgggaa tgtattcagt ggtcgttgat caagcagtta accatttcac aggaatacca
     2761 attaaatggg attatgatgt ttcggaacag aaaaggacat tcattcaacg aatgagagac
     2821 aaattactgg gtaatgatat cagcgcccct tctaccccag aagaatttga aacactcacg
     2881 agacaattaa gtactatgcg gtttggaatg ttagaccccg atactgcaac atttcaagga
     2941 gcgacagggc ttgctttcag attactggag cctgttgaga caaatgatgg ttgggaacta
     3001 agagcaagca acattgagcc gtggaaagct gaaaagtacg aaaacggcgc agtgtacatt
     3061 aaagagaaat acgatccata tcaaaagaag ttttatgaag agatgaaggt tgtcacgccg
     3121 aaatcaatat ttacttatgc tcgatatact gattaccctg gcacggtaca aggagcattt
     3181 aaaacgctgg gtaatgttcc taatccttta ggcgttatca atcttacgga gtttaaaaac
     3241 aatacaaacc gttactgtga ctttgaagtt gcagaggaga ttagtgatgc tattgatcgt
     3301 gttttatctg atcagcagaa cgagattgaa caattcaagt tagcttatat gctgatcact
     3361 ggggcagatt tgtccgagga taaagccaag aagatgatgg agcaactagg gatcatcaat
     3421 ctgaaagacc ctaatgcgaa agccggctac gtgacaaagg atctacaaaa ggatttcaac
     3481 gagtatcatt ttgatcttct taaaaagcaa ttctacacgg tgtgtaaagc aattgatttc
     3541 aatgacgaag tctttaagtc gaatagttct ggggaagccc gaaagtggca aatcattagt
     3601 ttagaagcta agacaaatac caaagaacag tactttcgag aaggattgaa agagtgtgcc
     3661 gaggttatgg ctgcttattt ggattttgcc gagaaagtta caattgatcc ggaaaagatc
     3721 attttcacct tctctcgttc gcttccaaca gatttaagct acctagctga ggcattgcct
     3781 aagttggcac catacgtttc taagaggacg attcaaagtc agatcccgtt cgtcacagat
     3841 gttgattatg aaaacgaaat gattgacgtc gaaagcagac aatcttaccc agacggcgag
     3901 tatggaggtg gcatcgttga gtcaaataga aacgaagtac tggaccaaac gtcgagaact
     3961 ggaagattta gcaagactca aaatggaaaa tcaaacgcta aagaaactaa gtagtgtctt
     4021 tcctgatgcg ttgaaagaaa ttcaagcgga gttattatcc caagctgatt tacacgatat
     4081 tacctatgct gagatgatgg aattttatag tacaagtgat cagaaaaaat atcgtgaata
     4141 cgttgaaaag aactacaaat cactgaagat gtatgatgca aaatacaaag agttcatcga
     4201 agaatacttt ccttctttcg actacgccaa agtgaatcgg ctgcttcaaa tgaggtcaga
     4261 tatttttcga attcttgcag agagtgcaat agatgcagat gtgaataaat atttttcgga
     4321 tcggttagaa gatatcttgc aacgttcgta tgtatcaaac gcaaacgtat ttgcccagat
     4381 tttgaacgta gatttgccga attacttatc aaaggaagaa cttgaacgtt acctgaacta
     4441 tccttggtca ggtaagacat tctcgcgtcg tttatggggc aatatttcat ctctagaaca
     4501 aaagctatct aacgccatta caactagtgt agcaagtggc gaaggagttc tcgtggcatt
     4561 acgaacaatg cgagaagacc cagaaatatg cgatatgttc aaaagggagg aatcgaagta
     4621 caacaaggcg atcgaaaatc ttgtgagaac agaatacgct aaattcgctc aagatggtat
     4681 tgagaaatct tatattgaaa ctggcgtaga cgaatacaat gtcttaactg caaaagatga
     4741 aaaggtatgt tcaatatgcg gtggcaaagc aaagaacaac ccatacaagt tgagtgaagc
     4801 gatcattggt gagaaccgtg cgccttttca tggccgatgc agatgtacag atgttcctaa
     4861 tatgccgaaa ttaggaaaag atattgacga agagtatgag cgtttatttg gcgatttatt
     4921 agatgagttt gcacatgatt cttttggaat taatttgaaa cggaggaagt agaatgaaag
     4981 atttttttga agcagtacta acaattaatg taaatgctga tattgcagaa gcctacaaaa
     5041 cagctattga gtctgagaac catcctaatg gcttgagaga ccattggaat ggcaattatg
     5101 cctacgtggt tattggcgat caaactgtta attatcaaga taatactcca gttgataaga
     5161 ataccgttaa tttaacgatt caattattat ctcattcatt accaaattta aaagaaacag
     5221 ttgattggta tgaaaatatg ggatgtattg ttgttagaac tgactacaaa gaaggaaagt
     5281 ctagtaatta ggctttttta ttttgtccga aatgacgtta aactagcgca atactgggct
     5341 tggttgaatg gtggggcgca actattaaaa ctcaaagcaa tgcggggcgt gaaaacgaat
     5401 cgtggggcga aaggagaatg attatgaaac acaaatcatt aatgccaatg aatttgcaat
     5461 actttgccga aggtgacgat caaaagtttt cttttgatga cttcaaatct tttgtggaat
     5521 ccaatgaaga agctcaaaaa tttattcagt cacaatctca aagtgttgcc gacaaacaat
     5581 tggaagcttg gaaacaaaat aacttagata aaattaaaca ggataccatc aaggaatatg
     5641 aggaatctaa gaaaaacaag tcacctgagc aaattcaact ggaaaaacta caagctgaat
     5701 ttgaagcaga aaaagcgttg cgtgtgacaa gtgataataa agcatttgtt gcagaacaaa
     5761 ttgctggatt agaactagat ggagagttaa aagagtctat ttctcaattt atgctaaata
     5821 atcttgttag ttcggataca gatttcacta agaatgctgt tgaaggtttt acaagtgttt
     5881 tgaatgcaat taatgagaaa catgcagatg cattaaaaga actacaaatg aagtctgcat
     5941 ttggtggaac tcaacaatcg aataaccagg ttcagcagaa caatgaaaca ttaacaaatc
     6001 cagaagaaca attaggacaa attcttcaac aatttaacta ggagagtgaa aaattatgaa
     6061 aaaaacatct ttaaataatt tagagtattt ggatatttca ccagcgatta atgctatgca
     6121 agtaccaaat acaccttttt taagctattt acttggtgct ggaaaaacag agccagcaaa
     6181 ctcgacagaa attaaatggc gtgaatatga tatcaacaac gatgattctt ctgaaaaact
     6241 tgagggcgga gaatatccag atgctgaatc aggtcgaact tggtttaaca actatactga
     6301 aatttttaga aaatcaacct ctgtatctgg tacattagat gctattaatg tgaatggagt
     6361 cggaaatgaa ttaactaatc aagtagcact acgtggtatg gaaatgaaaa ttgatttgaa
     6421 ccgaaaattg attactggtg taaaagctga tgaaaatggt tctaaaggtc gtcgaatgaa
     6481 tgggattttg aacttgatca attcagcaaa taaggcagaa acagccactg cgggtgcagt
     6541 aacaagaaaa gatatcgata ctttatttaa attgatgtat gaaaaaggtt atatgggaga
     6601 aaaactatgc ttgatttctc cagatatgca ggagttaatg actgatgagt tagatggaaa
     6661 atcaacaaaa attgttcagt tcggggaaag agtaactttt ggattgcaaa ttggaaatat
     6721 cgtgtctaat tacggtacag gtattgctct actagaacca tcattgccaa aaggaacaat
     6781 tgccgcaatc gatactaatt atgtgaaact acgtccatta cgtgaatgga gagcagaaga
     6841 acttgcaaaa acaacggatt ctagacgtat cggtcttgta ggtgaatact ctcttgaata
     6901 caatgcttca aactctgggg caattttaaa tttaaagtct gaataaaagg gagttagtac
     6961 tccctttttt gataggagga attacagtgg taaaaaaaga tgaaactaaa aaagatgaag
     7021 tcgtgaaata tagagtaggt aaaactaaaa attttgttgg gtttgttcat cctaaaactc
     7081 gtagatttat cacagcagat tcaaataacg aatttatcat ttctatagat gataaagaag
     7141 caattgcaat tttggaagat gcaattgatg ttaatgaaat ttaggaagtg atctgatgga
     7201 tgaatcgcta aaaacggaaa tcattgagtc tacaaaagaa gattttccag atttgagtga
     7261 agaacgcata actaatttat tagaaataat tttgctagaa attgaatcat acaacacttg
     7321 taaaaatgat atttcatggg aaaagttaaa aagcgtgatt agtgaagtgt tgtataaaat
     7381 aataaaaaat gaatcagaaa aaacagtatc ttcagttaga cgtggcgata caacgattag
     7441 ttatgcttca acaacaaatg atgttagtga gttacttcta ggctatggtg atttgatacg
     7501 aagagttatt ggttgtggag gattggagtt tttttaatga atgaagcaga tattttagaa
     7561 actacttatg aagatagttg tattattgaa agactgacgg acattgaaga ttctaataca
     7621 aatattacta ttcaagatta caaaaaagta tatgataatc ctatttcttg cgctctttca
     7681 caaggtcaaa tcgatggact agcagtcata gaagatggag agatggtaaa tgtttcaact
     7741 gacacatata aattatttgt tcatcctaag attaaactaa aaaaaggaga tcgaataaca
     7801 ataactcaaa aggccagtgg cttaattttt tctctatttg ctactaagcc tttttactat
     7861 cctagtcatt gtgaagtgaa tttgatagga agtgagaaaa atgggtaatc tcaaatatga
     7921 atcaaatgca gaaaagatca ttgagaattt taaaaatatg actgtaattg ctcaaaaaga
     7981 aggaatatcc ttcgttaacg attcaatgaa taaagtcgtt agtttagcta aaccattaac
     8041 tcctgtaaaa tcaggtaatt taaggcgagg atatcgtgta gtaaaagcta gaaaactatc
     8101 aagcggtcga attgttggag cagttataaa caatgaacat tattttaaat atgtagaaga
     8161 aggtcatagg actaaaaatg gtggctttgt caaagggaaa ttcatgttaa ctcgtgcaac
     8221 gaatcttgca aatatgtctt atattcctcg aagatttaaa caaatggcaa taaaaatcgt
     8281 taagaaagga aagtaacatg aaagatgaaa tcattgctgc aatcagtagc aaattaaaag
     8341 aaatctatcc tgatgggaca atatatcttg attcagttat gcagtcaact aaagattttt
     8401 atttcgtttt atcagtaatg gaatctggaa ctgaaaatgt aggaattgat attcaaaatg
     8461 tttctttctt aattgatatt gcattgattg ataataaacc taatagaaat ttaattaatg
     8521 aattagtctc acgttgtggg acttttttta atacgattac aatcgatgag caaatattat
     8581 ttccaaaagc ctatttacct gatgaagcag atggtgttca acatattcgt tttacattag
     8641 aatttccaca atatattgaa tggagtgaaa gataaatggg agaaaaaaga agtaaaaccg
     8701 gaattatttc tgtcgaaaaa cctacttggt ttcctttgga agatgagaca gggaatttcc
     8761 cggtatatgg tactgcgttg ccaatgggaa cagcagtaag tatcaaacca acagctaatt
     8821 atgaaacaac acaagattat ggggattcag tggttcaaga tcaatttaca gcttttggtg
     8881 gtgcagaagt tgaactggaa gcaaatggat atactcataa agttttatct acaattactg
     8941 gagggaaaat tgttaaaggt ggtgccttgc gttctggaga agatattgcg caagatggtg
     9001 cgtttgcata tagacgtaaa aaatcgaatg ggaaataccg ttatacggtc ttttataaag
     9061 gacagtttgc tctagattca gatgaaacgt caacgattga aggaagtaaa gtaagtttta
     9121 ctcatccaac ttggaagggt tcatttgtag atgtaccagg acttggatat atgtattcag
     9181 ttgatgaaga tgatgaaggc gtggataaag caatgatcgg aaattggttt acaaaagtag
     9241 caattccaat tgaagaagca gaactttcag gaggtacaga ataatgtcaa aatatcaaac
     9301 aacgattaaa ttaacgaaaa aagatgaaga aggtaaatat gagcaagtac aatttaaatc
     9361 tgctgaattt ttaccaggaa cagttgtaga agatgctgca ggagttatgg aagaaatgca
     9421 aacagcgact gataaacaat cagttaaaaa agctttaagt cgtgcttatt catttattgc
     9481 agatacttta tttgagggac aatttactgg tgaagattat tgcaaaggaa ttgatgctcg
     9541 tgagattgcc tcattgacag gaaaattatt gaagtctgtt actgcaggtt ttgatgaaac
     9601 ttatacagag acgaaaaaaa agtaagtgag gcgctcaagt caccttcatt taagtattcg
     9661 attacttacc gagaattaga tataaagacg caattactcg aagcaggttg gacgttacca
     9721 gaaattgaac gtactgactt ggatgagtta atgcgtcttt ttgcttttag agatgcggta
     9781 aaagagcatg aagatgttga gtactatgat aatttcactc aattttagga ggtgatactt
     9841 tgaataacga tgacctaatt ctgaagatga tactagatga atctggtttt actgccggta
     9901 tgaataatgc tgtcaaaaag ttgaactctt ttgatgaaac aatcgaaaga aaaagtagaa
     9961 atagtggcag ttcattaggt agtatttgga aaatatttgc tggaagtttt ctagctagcg
    10021 gagtaactag aatcgtagga gctggctttg atctaatcaa gggttccata agtggagcgg
    10081 ttgatcgagt agatacgatg aataacgctc tacgaaattt ccaaaatatg ggatttagta
    10141 attcagaaat tatgaagaat atcggaaaga atgggctttt atctcaaggt attcaaggac
    10201 ttcctactgc tttgaatgat gcgataagtc atgttcaact tcttgcttct tctacaggtg
    10261 atttaactcg ttcaactcaa atatttaaag ctttaaatga tggaattctt ggtttcggtg
    10321 gttcaactga tcaagttaat gaagctgtta ttcaattatc tcaaagtttc tcaaacggaa
    10381 aagtagacgc acaaacttgg aactcaatga tcaacgctca acttggacct actctttctg
    10441 ctattgctaa aaagatggga attacaatgg gagatctgaa agaaggtttg tctcaaggta
    10501 agatttctgt tgaagaattc caaaatcaat taatagaaat ggataccaaa ggtggcggag
    10561 gacttaaatc attaagtcaa atcgctaaag attctactaa gggaattaaa acctctatac
    10621 aaaatgcaaa aacagctgtt acacgtggtg ttggtgaggt aatagaagga ttaaataaag
    10681 cattagtaga ttcagattta ggtggattta agggaattat tgataaagtg ggcagttcaa
    10741 tggaatcgtt cttaaaagta attgctgcaa atattcctac agcagtatca tttttaagta
    10801 atttatttaa cgaaattcaa aaatttggtt ctgctttgaa attcatgatg ccctttcttg
    10861 ttcctgcagc taccgctttt ggagcgttca tgtttcaact taaaggtata ccagcaataa
    10921 taaaaagctt caataatttt aagaatgcca taattggcgt tggaaattca ttaaagatta
    10981 tgggggcaat agctgctgct aattcatttg ttttaattat tggtgcagtc gttggtgcaa
    11041 tagctgtttt cggttacttt atggcaacca atgaagagtt tagaaacaag gttatatcta
    11101 tttggaatga tgtgaaagat tccgtaattg gtgtattaaa aaatataaag gactggggaa
    11161 ttgatacttg gaattctgct aaagaaatgg catcaaatgc agttgagggt gtcaaagatg
    11221 cttggtcagg aacaaaggaa tggttttcta acacctggaa aggtattaag gatggggcca
    11281 caggtttatt tgataaaaca gtagagactt caagaaatgc agtcgatagt gtaaaaaatg
    11341 catggtctaa tatgaagaaa tggttttctg atacttggca aagtataaaa gattcagcca
    11401 gagaaaaatg gaatgaaatt aaaggttcta ttatgtcggt tgctggtcca ttgattacag
    11461 gtataaagaa tgcattttta catgttactt tttatctaga gaccttatgg aataatttag
    11521 ttgagattgg taaaaacgtc tttgagattt taaaaaatgt tatacttgcg ccggttttgt
    11581 ttattacctc catgatttct ggtggatggg aagaaacaaa aaataatatg attgctgttt
    11641 ggaataatat taaagaaagt gccataaata tttgggaatc tataaagaat atatttgtaa
    11701 gttattttac aaatatttat tttgctgcac tcaatatttg gacagggttc aagcttacat
    11761 tgataaatat ttggaatgaa gtggtaaatc aagctaaatc gatttggtta aacataaagt
    11821 atttttttat taatctttgg attgatataa aatattttgc aattcagaaa tggattgaat
    11881 tgaagttttc tattattcaa acttggatcg atttaaaata taatgccatt actacttgga
    11941 ataatattaa acagtttttc aaagatactt ggaaaaatat taaa
//
LOCUS       DACEQH010000074        11347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.076, whole genome shotgun sequence.
ACCESSION   DACEQH010000074 DACEQH010000000
VERSION     DACEQH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.076"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..936
                     /locus_tag="IW833_002337"
     CDS             <1..936
                     /locus_tag="IW833_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1308426.1"
                     /translation="GRWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESL
                     AIDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADH
                     IESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVA
                     MLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFV
                     TQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQC
                     LISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3122
                     /locus_tag="IW833_002338"
     CDS             939..3122
                     /locus_tag="IW833_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1308427.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3119..4120
                     /locus_tag="IW833_002339"
     CDS             3119..4120
                     /locus_tag="IW833_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1308428.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4135..5049
                     /locus_tag="IW833_002340"
     CDS             4135..5049
                     /locus_tag="IW833_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1308429.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5294..5410
                     /locus_tag="IW833_002341"
     CDS             5294..5410
                     /locus_tag="IW833_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1308430.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5426..7345
                     /gene="tet(M)"
                     /locus_tag="IW833_002342"
     CDS             5426..7345
                     /gene="tet(M)"
                     /locus_tag="IW833_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1308431.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQREVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7443..8816
                     /gene="tet(L)"
                     /locus_tag="IW833_002343"
     CDS             7443..8816
                     /gene="tet(L)"
                     /locus_tag="IW833_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ1308432.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9380..10642
                     /locus_tag="IW833_002344"
     CDS             9380..10642
                     /locus_tag="IW833_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ1308433.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10887..>11347
                     /locus_tag="IW833_002345"
     CDS             10887..>11347
                     /locus_tag="IW833_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ1308434.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINK"
BASE COUNT         3741 a         1897 c         2377 g         3332 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt caatctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtaa ttatgaaaaa taccaaagat
      121 tctgaatcac tggctattga tattttgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtgcgaggg
      241 ttgatgaaag tgattgaaga attacgggtt gagaatacgc cactaagtac cagcatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcccatc tgctttttag tgatggttat
      361 gtagagcagt ctatcagcct tgaaaaacaa ctgaacatta tacaggttgc cgacttggta
      421 cttcctgaca aggaaacttc ctttgaggaa tataccacaa tggagttact atcggtagca
      481 atgctgattg tcattagtac ctttgcgttg gactttatcc atacagaccg aagtattttc
      541 aagattgtag acttagacga agcatggagc ttcttacagg tggcacaagg gaaaacacta
      601 tctatgaagc tggttcgtgc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagatg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgccttt
      721 cgttccactg accttaacga gattaaaaaa acccttgcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atattaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggctacc gtggaactgg tcggacggaa
     1201 ttggaaaaca agtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtc
     1261 tttatgtttc caatgcgacg ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggcaagaata tgcagaccct tgcaggggtt acgcctagtg
     1381 gactatcaac agagggtttc tatgttggat tccttttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacagga ctgataaaga gagaaaccac aaaggcaatt catgccctca
     1501 tgaattttgt gctggttttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagcaacgc tagtttgtca ctgggtacaa
     1621 aaattgtcat gccccattca gatagtcaag gcaaggacag tgtggacttg atacgtgaca
     1681 gcctattttc catacaggtt cagcaaccgt ggctactgct tcaatataac agttcagaca
     1741 ttgaaagtat tggtattgac cgtgtggaaa gcctgctctc aaccagtcca gattccaaca
     1801 atggggaaga cagagaaaaa attgttgcgg aagaaattga agatagaagc aataccaata
     1861 tgacgattac aaagacgatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttta
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtatgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ctccgtgact gctttagggg ctgggtctgc cattgccagt gctacgggta
     2401 aaaaaggaca gtcgggttcg ggaagttctg cgagtacaca agcagatcac tctcgaccaa
     2461 acgggcagga aaaatcaaca ctaggaaagc gtatcggtca aaccatcggt acagtagctg
     2521 ataccaaaga cagaatggta gatactgctg gtaatttgaa agaacaggtt aaggatttgc
     2581 cgaccaatgc aagatatgca gtatatcaag gaaaatctaa agtgacggat aatgtccatg
     2641 atttaaccag aagtatttct caaaccaaag cggataaagc cagtggacgc aagaaacagc
     2701 aggaacaaag acggaaaacc attgcggagc gtcgctctga aatggaacag gtcaaacaga
     2761 aaaaacagcc agcttcttcc gttcatgaaa gaccagccac taagcaagaa caatctcatg
     2821 atggacagac ttcaaggcaa acaactgtac aggcttccta tagggaatct caacaagcca
     2881 aacaagagcg tccaacagtt aagtccgatt cttcaagttt aaaaacggaa cgtcaaagta
     2941 atacagttca agaaagaacg gttcaaaagc cagtaacttc aactgcacca acagatagag
     3001 cttcacaacg ttcaatcaca aaagaacgtc agtctactgt tcaaagagta ccactacaaa
     3061 atacaaaaag tagaccacca atcaaaaccg ccaccattaa gaaaggcagt aagaaaccat
     3121 gaagctgaaa actttagtga ttggtggttc gggattattc ttgatggtct tctcactgct
     3181 tctgtttgtc gccattttat tttcagatga acaagatggc ggtttttcca acattcacta
     3241 tggcggtgtg gatgtttccg cagaagtgct ggctcataag cctatggtag aaaaagttgc
     3301 caaagaatat ggaattgaag aatacgtcaa tatacttctt gcgattatac aggtggaatc
     3361 gggtggtaca gcagaagatg ttatgcagtc ctcggaatcc ctcggtcttc cacctaattc
     3421 attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgagt tattagccag
     3481 tagcgaaagg cttggtgtag atttagaatc ggttatccag tcctacaatt atggtggtgg
     3541 tttcttagga tatgtggcta atcgtgggaa taaatatacc tttgaactgg ctcaaagttt
     3601 ctcaaaagag tattcgggtg acgaaaaagt gtcttacccc aatcccatag ccatacctat
     3661 caatgggggc tggcgataca attacgggaa tatgttttat gtgcaactgg taacgcagta
     3721 tcttgtcaca acagagtttg atgatgatac agtacaagcc atcatggacg aagctctgaa
     3781 atttgaaggc tggaagtatg tctatggcgg agcttccccg actacctctt ttgattgtag
     3841 cggactgaca caatggacgt atggaaaagc tggaatcaac ttaccacgaa cagcacaaca
     3901 gcaatatgat gtaacccggc atatcccatt attggaagca aaagccggcg atttggtttt
     3961 ctttcattct acctataacg ctggctctta tattactcat atcgggattt atttaggcga
     4021 taaccgtatg tttcatgcag gcgaccctat cggttatgcc gacttaacaa gctcttactg
     4081 gcaacagcat ttagtgggag caggacgaat caaacaataa gaaaggacga ttcaatgatg
     4141 aaattcggaa aaaatcagaa tacagaaaaa cagacaccaa aggaaaagaa acctcgtgtc
     4201 tataaggtca atcctcgtaa aaaggttgtg attgtcctat gggtactttt aggacttagt
     4261 ttcagctttg cgatattcaa gcactttaca gccattgata cccataccat tcatgaaacc
     4321 accatcatag aaaaggaata tgtcgatact catcatgtgg aaaattttgt agagaacttt
     4381 gcgaaagtct actattcatg ggaactaaac gacaagtcca ttgataatcg catagaaaac
     4441 ctgaaagcat atctgacaga ggaacttcaa gctctgaatg tcgatacagt tcgtaaagat
     4501 attcctgtat cgtcttctgt aaaaggattt cagatatgga cggtagaagc agatggcgac
     4561 aatcaatttg atgtaaccta cagtgtagac caactcatta cagaggggga aaatacaaag
     4621 accgtccact ctgcttatat agtgagtgtc tatgtagaca gtactggaaa tatggtcctc
     4681 attaagaatc cgaccattac tagcatacca aagaaatcag actataaacc aaaagctatt
     4741 gaaagtgagg gtacggttga ttccattaca accaatgaaa tcaatgagtt tttaacgacg
     4801 ttcttcaagc tctatcctac agcgacagcc agtgaacttt cctactatgt gaatgacggg
     4861 atattaaaac caatcggaaa agagtacatc tttcaagaac tggtaaatcc tatttacaat
     4921 cgtaaggata atcaagtcac ggtatcgctg acagtggagt atatcgacca gcagaccaaa
     4981 gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt
     5041 atagaataac aaatattggt acattattac agctattttg taatcacgta ctctctttga
     5101 taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta agtatctatt
     5161 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg
     5221 ggaacaaata attggatgtc cttttttagg agggcttagt tttttgcacc cagtttaaga
     5281 atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat
     5341 ggttatgcat aaaaatccca gtgataagag tatttatcac tgggattttt atgccctttt
     5401 gggcttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
     5461 tgatgcggga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
     5521 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
     5581 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
     5641 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
     5701 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
     5761 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
     5821 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
     5881 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
     5941 tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg
     6001 taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgctc
     6061 cttgtaccct gtttatcatg gaagcgcaaa aagcaacata gggattgagc agcttataga
     6121 agtgataacg aataaatttt attcatcaac atacagaaag aagtctgaac tttgcggaaa
     6181 tgtcttcaaa attgaatatt cggaagaaag acaacgtctt gcatatgtac gcctttatgg
     6241 cggaatcctg catttgcggg attcggttag aatatcggaa aaggaaaaaa taaaaattac
     6301 agaaatgtat acttcaataa atggtgaatt atgtaaaatt gataaggctt attccgggga
     6361 aattgttatt ttgcaaaatg agtttttgaa gctaaatagt gttcttggag atacaaagct
     6421 attgccacag agagagagaa ttgaaaatcc gctccctctg ctgcaaacaa ctgttgaacc
     6481 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
     6541 cccgcttcta caatattatg tggattctac gacacatgaa attatacttt ctttcttagg
     6601 gaaagtacaa agggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga
     6661 actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat
     6721 tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct
     6781 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
     6841 atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg
     6901 gaatgtgacg gattgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     6961 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
     7021 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
     7081 acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
     7141 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
     7201 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
     7261 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     7321 atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg
     7381 ttaaataaga tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggaattaa
     7441 ttatgaagtg taatgaatgt aacagggtta aatacaacca aattttaatt tggctttgcg
     7501 ttttatcttt ttttagcgta ttaaatgaaa tggttttgaa cgtctcatta cctgatattg
     7561 caaatgattt taataaacca cctgcgagta caaactgggt gaacacagcc tttatgttaa
     7621 ccttttccat tggaacagct gtatatggaa agctatctga tcaattaggc atcaaaaggt
     7681 tactcctatt tggaattata ataaattgtt tcgggtcggt aattgggttt gttggccatt
     7741 ctttcttttc cttacttatt atggctcgtt ttattcaagg ggctggtgca gctgcatttc
     7801 cagcactcgt aatggttgta gttgcgcgct atattccaaa ggaaaatagg ggtaaagcat
     7861 ttggtcttat tggatcgata gtagccatgg gagaaggagt cggtccagcg attggtggaa
     7921 tgatagccca ttatattcat tggtcctatc ttctactcat tcctatgata acaattatca
     7981 ctgttccgtt tcttatgaaa ttattaaaga aagaagtaag gataaaaggt cattttgata
     8041 tcaaaggaat tatactaatg tctgtaggca ttgtattttt tatgttgttt acaacatcat
     8101 atagcatttc ttttcttatc gttagcgtgc tgtcattcct gatatttgta aaacatatca
     8161 ggaaagtaac agatcctttt gttgatcccg gattagggaa aaatatacct tttatgattg
     8221 gagttctttg tgggggaatt atatttggaa cagtagcagg gtttgtctct atggttcctt
     8281 atatgatgaa agatgttcac cagctaagta ctgccgaaat cggaagtgta attattttcc
     8341 ctggaacaat gagtgtcatt attttcggct acattggtgg gatacttgtt gatagaagag
     8401 gtcctttata cgtgttaaac atcggagtta catttctttc tgttagcttt ttaactgctt
     8461 cctttctttt agaaacaaca tcatggttca tgacaattat aatcgtattt gttttaggtg
     8521 ggctttcgtt caccaaaaca gttatatcaa caattgtttc aagtagcttg aaacagcagg
     8581 aagctggtgc tggaatgagt ttgcttaact ttaccagctt tttatcagag ggaacaggta
     8641 ttgcaattgt aggtggttta ttatccatac ccttacttga tcaaaggttg ttacctatgg
     8701 aagttgatca gtcaacttat ctgtatagta atttgttatt acttttttca ggaatcattg
     8761 tcattagttg gctggttacc ttgaatgtat ataaacattc tcaaagggat ttctaaatcg
     8821 ttaagggatc aactttggga gagagttcaa aattgatcct ttttttataa caggaattca
     8881 aatctttttg ttccattaaa gggcgcgatt gctgaataaa agatacgaga gacctctctt
     8941 gtatcttttt tattttgagt ggttttgtcc gttacactag aaaaccgaaa gacaataaaa
     9001 attttattct tgctgagtct ggctttcggt aagctagaca aaacggacaa aataaaaatt
     9061 ggcaagggtt taaaggtgga gattttttga gtgatcttct caaaaaatac tacctgtccc
     9121 ttgctgattt ttaaacgagc acgagagcaa aacccccctt tgctgaggtg gcagagggca
     9181 ggtttttttg tttctttttt ctcgtaaaaa aaagaaaggt cttaaaggtt ttatggtttt
     9241 ggtcggcact gccgacagcc tcgcagagca cacactttat gaatataaag tatagtgtgt
     9301 tatactttac ttggaagtgg ttgccggaaa gagcgaaaat gcctcacatt tgtgccacct
     9361 aaaaaggagc gatttacata tgagttatgc agtttgtaga atgcaaaaag tgaaatcagc
     9421 tggactaaaa ggcatgcaat ttcataatca aagagagcga aaaagtagaa cgaatgatga
     9481 tattgaccat gagcgaacac gtgaaaatta tgatttgaaa aatgataaaa atattgatta
     9541 caacgaacgt gtcaaagaaa ttattgaatc acaaaaaaca ggtacaagaa aaacgaggaa
     9601 agatgctgtt cttgtaaatg agttgctagt aacatctgac cgagattttt ttgagcaact
     9661 ggatccagga gaacaaaaac gattttttga ggaaagttat aaattatttt ccgaacgata
     9721 tggcaagcaa aatattgctt atgcaacagt tcataatgat gagcaaaccc ctcacatgca
     9781 tttaggtgtt gtgcctatgc gtgatggaaa actgcaagga aaaaatgtgt ttaatcgtca
     9841 agaactgtta tggctacaag ataaattccc cgagcatatg aaaaaacagg gttttgagtt
     9901 gaagcgtggt gaacgtggct ctgaccgtaa acatattgag acagctaaat ttaaaaaaca
     9961 aactttggaa aaagagattg attttctaga aaaaaattta gcagttaaaa aagatgaatg
    10021 gactgcttat agcgataaag ttaaatcaga tttagaagta ccagcgaaac gacacatgaa
    10081 aagtgttgaa gtgccaacgg gtgaaaagtc catgtttggt ttgggaaaag aaataatgaa
    10141 aacagaaaag aaaccaacca aaaatgttgt tatatcggag cgtgattata aaaacttagt
    10201 gactgctgcg agagataacg ataggttaaa acagcatgtt agaaatctca tgagtactga
    10261 tatggcgaga gaatataaaa aattaagtaa agaacatggg caagttaaag aaaaatatag
    10321 tggtcttgta gagcgattta atgaaaatgt aaatgattat aatgagttgc ttgaagaaaa
    10381 caagtcttta aagtctaaaa taagcgattt aaagcgtgat gtgagtttaa tctatgaaag
    10441 cactaaggaa ttccttaagg aacgtacaga cggcttaaaa gcctttaaaa acgtttttaa
    10501 ggggtttgta gacaaggtaa aggataaaac agcacaattc caagaaaaac acgatttaga
    10561 acctaaaaag aacgaatttg aactaactca taaccgagag gtaaaaaaag aacgaagtcg
    10621 agatcaggga atgagtttat aaaataaaaa aagcacctga aaaggtgtct ttttttgatg
    10681 gttttgaact tgttctttct tatcttgata tatatagaaa taacgtcatt tttattttag
    10741 ttgctgaaag gtgcgttgaa gtgttggtat gtatgtgttt taaagtattg aaaaccctta
    10801 aaattggttg cacagaaaaa ccccatctgt taaagttata agtgaccaaa caaataacta
    10861 aatagatggg ggtttctttt aatattatgt gtcctaatag tagcatttat tcagatgaaa
    10921 aatcaagggt tttagtggac aagacaaaaa gtggaaaagt gagaccatgg agagaaaaga
    10981 aaatcgctaa tgttgattac tttgaacttc tgcatattct tgaatttaaa aaggctgaaa
    11041 gagtaaaaga ttgtgctgaa atattagagt ataaacaaaa tcgtgaaaca ggcgaaagaa
    11101 agttgtatcg agtgtggttt tgtaaatcca ggctttgtcc aatgtgcaac tggaggagag
    11161 caatgaaaca tggcattcag tcacaaaagg ttgttgctga agttattaaa caaaagccaa
    11221 cagttcgttg gttgtttctc acattaacag ttaaaaatgt ttatgatggc gaagaattaa
    11281 ataagagttt gtcagatatg gctcaaggat ttcgccgaat gatgcaatat aaaaaaatta
    11341 ataaaaa
//
LOCUS       DACEQH010000075        11321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.077, whole genome shotgun sequence.
ACCESSION   DACEQH010000075 DACEQH010000000
VERSION     DACEQH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.077"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(40..1419)
                     /locus_tag="IW833_002346"
     CDS             complement(40..1419)
                     /locus_tag="IW833_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HAQ1308435.1"
                     /translation="MTKVTLKKVNPPKKDNEAKIYQLTDETMKFRKEKVLAAMEKSEI
                     DTLVIYCDLEHGGNFSYLTGFVTRFEESLLVLHQTGEAFLVLGNENTKMVDYSRIEAE
                     LIHTPLFSLPDQPMDNEKPLTEVLTNAKISKGKKVGLVGWKMFTSEEDNYQIQDLPAF
                     IVSSIVELVGEDAVSNRTDLFIHSDYGVRSLNTANEVAYYEFGSSLASDCVMNAIDEV
                     EVGKTEMEIGNHLVKYGQPNNVIPIAATGERFENAYIYPTDKQVKLGDKMSITTGFKG
                     GLASRSGYAVATTDDLPEGQKDYIEKVAAPYYDAVVAWLENIKIGMQGKELYSVIGKV
                     LPKSEYKWHLNPGHLTADEEWMSSPMKKDSEITLKSGMLLQIDIIPSVAGYAGASCES
                     GIALADESLRDQLAKEYPNVWSRIISRQEYIRNELGIELPDEVLPLSSGVGYYTPFFL
                     EKNLALVKG"
     gene            complement(1440..2987)
                     /locus_tag="IW833_002347"
     CDS             complement(1440..2987)
                     /locus_tag="IW833_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1308436.1"
                     /translation="MGPLSIIVASGLLLGIVSILQNPTLVGESFANATVIQGFVGGVQ
                     AIVGTMFGLLPILFAISVAIGMSREDKEIAAFSVVIAFILFHVVINYLLQLRGITAES
                     TSVETLMSDGMSQIKAVETSNMYETLLGTFTYRMGVFGGIIAGLWTAMIHNRYHTKQL
                     PAAFSFFSGNRFVPIMIILTIPIISIASFFIWPFVNTAINALGGLIGKSGVIGTFLYG
                     FSERLLIPTGLHHVLNQLIRFTPIGGTATINGETVSGALTIFNTELALPVKDMDILRS
                     ATRFLTQGTHPFMVFGLPAACYAMYKTSYLAQRPKIKGMLIAAAATCFVTGITEPIEF
                     AFIFISPILWVFHAFMAGLSFMLMNLFGVAVGNAGGGFIDLAIFGILQGTYTRWYLVV
                     ALGLVYAVAYYFVFKYVIERFDVKTPGRSNDDVLEDQDEEMTENELGKLILQGLGGAG
                     NIQEIDNCISRLRLVLKDTATIDEHILKKTGSMGIVKINENNLQVVYGGQVENATRSL
                     KNEMKRK"
     gene            complement(3236..4015)
                     /locus_tag="IW833_002348"
     CDS             complement(3236..4015)
                     /locus_tag="IW833_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1308437.1"
                     /translation="MTLEQLVNEHHSKLNENDDYVLSFITQNMEISISQTVSELAKQA
                     NVSDSTIIRLTKKLGFKGYGEFRYYLKEEFARMQKTRVDKSSLFLPEVLLGDVQATTK
                     LFEDDKSVEDIYETIRKADKIFAYATGYGQRMMLNEFARCMWNLGIYIIVVPEKNELD
                     LISKALTSNDLLFVISLSGRVDRIDDTLRRIHMKGTQLVSVTRFGQNNLASLSDYSLY
                     YQVTNINDVNELNNSSFCTLNLALSLLYEGYINYYKQSSVD"
     gene            complement(4090..4548)
                     /locus_tag="IW833_002349"
     CDS             complement(4090..4548)
                     /locus_tag="IW833_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1308438.1"
                     /translation="MGFFSRKEKLVAPVSGNLIPLSQVSDPVFSQGMMGEGFAVEPTN
                     AKVVAPIDGVIKSIFPTKHALTLTSNQGRDLLIHIGIDTVQLKGVGFQVLVEEGQRVS
                     SGESLVIFDCDQIKAEKLSDVVMVLFPEDKGIKLEVSEKKVSEGELLFKI"
     gene            complement(4560..5819)
                     /locus_tag="IW833_002350"
     CDS             complement(4560..5819)
                     /locus_tag="IW833_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HAQ1308439.1"
                     /translation="MKTNFERIKKDIENLAEYNATPGEGVTRSAYSKEDREARNYLIN
                     QMEQLGLEIWEDGFSTLFGRRAGKKADAPSVMIGSHYDSVVNGGRFDGVAGVVTALET
                     MRILEENNFENDYPIDLILMNAEEGETFGPSTGVSNSRAMMGTLTMSELETVKNRHGQ
                     TKLEALRAYGVEPDLEAAKRDPKTIKNFIELHIEQGPVLEDEGKDIGLIEFIPGIGRF
                     KARFYGETEDSTAPMNKRKDALVAASKFVLFVEQWMKNLGEGIAGTVGKLTIKPNSNQ
                     FVPDFVEALIEIRTFDTKTTNPQEIKEKLLNELSVIQGETGVRTELEEMARINYSNPT
                     NPSVMDQGNVEKMKHICDQLGYSYQVINNGTGHDAMMMTDFVPTNMIYIPSKGGITHH
                     PDEWTDFEDVKKGADVLLNLVMDISKD"
     gene            complement(5935..6249)
                     /locus_tag="IW833_002351"
     CDS             complement(5935..6249)
                     /locus_tag="IW833_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1308440.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPWICVNKVDAFIESFLNQSTLELCYETRKSSYGGIYDN
                     DF"
     gene            complement(6395..7891)
                     /locus_tag="IW833_002352"
     CDS             complement(6395..7891)
                     /locus_tag="IW833_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ1308441.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKPFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYCIHSDIT
                     DCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLMD
                     FVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLSD
                     LNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNNKWKFKLSLQKHLLQVKL
                     LGDKYPNCGSLSKALSEVYTHRVSTLNKRPDDIYQLISILVNIMQKNPRTLEACIVIL
                     GKLFEFIAVEEINLYLDKILRKFVNTPNTDIVEIWLQRLSLIHSREKSYSAKLCKKVS
                     NPKGFTLWNSDWLKKGFDESGIVNETFISNLSLTTPAKDLELFISQYE"
     gene            complement(8069..8548)
                     /locus_tag="IW833_002353"
     CDS             complement(8069..8548)
                     /locus_tag="IW833_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HAQ1308442.1"
                     /translation="MKVYTVQPVDFLQSVDSEGFIYPKKRYTELSVFFDNPNAVGALH
                     WLEEEYTKRIQLPLDRDLIWLWRDTCHLKYARKDNFTDFSKYNFYCFEIPIEFYRKNI
                     LWSRFIEWHEPLNHEWETDENQEFEHIFDIPKHPGYGDIQGVTTRLHKDWIKKITHN"
     gene            complement(8924..9169)
                     /locus_tag="IW833_002354"
     CDS             complement(8924..9169)
                     /locus_tag="IW833_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1308443.1"
                     /translation="MLFLKEEKLIDKENVIGSNIRRIRLEKGMGQTELVRELQLSKIA
                     MTRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            9274..10047
                     /locus_tag="IW833_002355"
     CDS             9274..10047
                     /locus_tag="IW833_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308444.1"
                     /translation="MKKFAIILTCALLTGAISGCATSDTNSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSESNSIEKVLPIEIEKNQENIKLKAELPILNSTEIDW
                     HENIKVTIEKGALAQLYTEKVEQFFESGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLETPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEADMVIKIF"
     gene            complement(10276..10524)
                     /locus_tag="IW833_002356"
     CDS             complement(10276..10524)
                     /locus_tag="IW833_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1308445.1"
                     /translation="MDTITPTNFKKNIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMKNEKEDDFKVV"
     gene            complement(10623..10937)
                     /locus_tag="IW833_002357"
     CDS             complement(10623..10937)
                     /locus_tag="IW833_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308446.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFRLFRDQIIGISQT
                     TK"
BASE COUNT         3515 a         2326 c         1697 g         3783 t
ORIGIN      
        1 tcatttcttc cgttttaaag aagaaatgat tttcatagat caacctttaa ctaaagctag
       61 attcttttct aaaaagaatg gtgtgtagta gcccacaccg ctggacaacg gtaaaacttc
      121 atccggcaac tcgatgccca gttcgttacg gatgtactct tggcgagaaa tgattcgcga
      181 ccaaacattt ggatattctt tggctagttg atcacgaagc gactcatcag ctagcgcaat
      241 cccactctca cagcttgcgc cggcatagcc agcaactgat ggaataatat caatttgtag
      301 taacattccg gacttaagag taatttctga gtcttttttc attggcgaag acatccactc
      361 ttcatcagcc gtaagatgcc ccggatttaa gtgccatttg tattccgatt taggcaagac
      421 tttgcctatt actgaatata gctctttacc ttgcattcct attttgatat tttccaacca
      481 tgcgaccacg gcgtcgtagt atggagctgc aactttttca atgtaatcct tttgcccttc
      541 tggtaggtca tctgtcgttg caactgcata gccacttcga cttgctaatc cgcctttaaa
      601 gcccgtagta attgacattt tatcgcctag tttaacttgt ttatcagttg gatatatata
      661 ggcgttctca aaccgttccc cagttgcagc aattggaatg acattatttg gttggccgta
      721 cttaactaag tggttaccaa tttccatttc tgtttttcct acctctactt catcaatagc
      781 gttcatcaca caatctgaag ccaaggatga gccaaattca taatatgcca cttcattagc
      841 agtatttaat gagcgtactc cgtaatcact gtgaatgaac aggtcggtgc gattgctaac
      901 ggcatcttcc cctactaatt ctacaatcga acttacaatg aatgccggca aatcttggat
      961 ttggtagtta tcctcttctg aagtaaacat cttccaacca acaagcccaa ctttcttacc
     1021 tttactgatt ttagcattgg tcaatacttc agttagtggt ttctcgttat ccatcggttg
     1081 atccggtaat gagaagagtg gcgtgtggat taattctgcc tcaattcttg aatagtccac
     1141 cattttagta ttttcatttc ctagaactaa gaaagcttca cccgtctgat gtaataccaa
     1201 caagctttct tcaaagcgag taacaaaacc tgttaaataa gagaagttcc cgccatgttc
     1261 caaatcacaa tagattacga gggtatctat ctctgacttt tccatcgctg caagtacctt
     1321 ttctttacgg aacttcattg tttcatcagt caattgatat attttagctt cattatcttt
     1381 tttaggtggg ttaacttttt ttagagttac cttagtcata ttgtcactac tttctgtttt
     1441 tatttacgct tcatttcatt tttcagagat cgagttgcgt tttccacttg tcctccatat
     1501 accacttgta aattattttc gttaatttta acgattccca ttgagccggt ctttttcaag
     1561 atatgttcat caattgttgc tgtatcttta agaactagtc tcagccgaga gatacagtta
     1621 tcgatttctt gaatattacc agcaccacca agtccttgca agattagttt gccaagttcg
     1681 ttttctgtca tttcttcgtc ctgatcttcc aacacatcat cgttggatcg accaggagtt
     1741 ttcacatcaa atcgctcaat gacatactta aatacaaagt aatatgcaac tgcataaaca
     1801 aggcccaatg ctacaactaa gtaccaacga gtataagtac cttgtaaaat tccgaagata
     1861 gccaaatcaa taaatccacc accggcattc ccaactgcga ctccgaatag attcattaac
     1921 ataaaggata aaccagccat gaatgcatgg aagacccata gaatcggtga gataaagata
     1981 aaggcgaatt caattggctc agtaatcccg gttacaaaac aagtagccgc tgctgcaatc
     2041 aacatccctt taatctttgg acgttgcgcc aaatatgaag ttttgtacat tgcatagcat
     2101 gctgccggca atccgaagac cataaatgga tgagtacctt gtgtcaagaa gcgagtagca
     2161 cttctcaaaa tatccatgtc ttttactggc aatgctaatt cagtattgaa gatggttaat
     2221 gctccagaaa ctgtttcacc attgatagtt gccgtaccgc cgattggtgt aaatcgaatc
     2281 aactggttta gtacatggtg aagacctgtt ggaatcagca ggcgctctga gaagccatat
     2341 aaaaaagttc caattacgcc tgacttacca atcaaaccgc ctaaagcatt gatagctgta
     2401 ttgacaaaag gccagatgaa aaatgaagcg attgagatga ttggaatagt taggatgatc
     2461 ataattggaa caaaacgatt gccactaaag aaactgaaag ctgctggtaa ttgttttgtg
     2521 tgatagcgat tgtggatcat ggctgtccat aatcctgcaa tgataccgcc gaaaacaccc
     2581 atacgatatg tgaatgtgcc aagtaaagtt tcatacatat tggaagtttc tactgctttg
     2641 atttgactca ttccgtcaga catcaatgtt tcaacagaag tgctttccgc tgtgatgcct
     2701 ctaagctgaa gcagataatt gataactaca tggaacagaa taaatgcaat aaccactgaa
     2761 aatgctgcaa tttccttatc ttctctagac atcccgattg ctactgaaat agcaaataga
     2821 ataggaagta gtccaaacat cgtaccaaca atagcctgta ctccaccaac aaatccttgg
     2881 atcacggttg cattagcgaa gctttctccc accaaagtag gattttgtaa aattgaaacg
     2941 attcctagta gtagtcccga tgcgacaata atagacaacg gacccattaa tgatttccct
     3001 aacttctgga tataagttct caatataaac accttcttct ttttagttga tataaacgtt
     3061 tacatccctt gtacaacgaa agtatagcac cctgaaaaag cgctaacaat acgataggtc
     3121 tgataaaaat caacattcag cacttggttg ttcattcaaa acgtgaatct tcaatcacag
     3181 aaaaatacgc gctatcaagc acgtatttta ggtgtaaata tatgcatcaa ctttattaat
     3241 caacgctact ttgtttgtaa tagttaatat acccttcata cagtaagcta agagcaagat
     3301 tcaaggtgca gaatgatgag ttgttcaact cattcacatc attaatgttg gtcacttgat
     3361 agtataggct atagtctgac agactggcta agttattttg accaaatcgt gtaactgaga
     3421 ccaactgtgt ccctttcata tggattcttc ttaaagtatc atcaatccga tcaacacgtc
     3481 cggacaatga tatgacaaat agtaaatcat tcgaggtcaa agcttttgaa atcaggtcta
     3541 attcattctt ttccggtaca acgataatgt agattcccaa gttccacatg cagcgtgcaa
     3601 actcattgag catcattctt tgtccatatc ctgttgcgta ggcaaatatc ttatctgcct
     3661 ttctaatcgt ttcatatata tcctcaacac ttttatcatc ttcaaacaat ttggttgtcg
     3721 cttgaacatc tccgagtaat acttccggta aaaacaaact acttttatcg actctggttt
     3781 tttgcatgcg tgcaaattcc tctttaaggt aatatcgaaa ttcgccatat cctttgaatc
     3841 cgagtttctt agttagtcta ataatcgttg agtcagacac atttgcttgt tttgctaatt
     3901 ccgatacagt ttgactgatg ctgatttcca tattttgagt gataaaactt aatacataat
     3961 catcattctc attcaattta ctgtgatgtt catttactaa ttgctccaat gtcataatca
     4021 gacctccaaa aataataaaa aagattgctc tctcatttta atacgaaagc acaatctttt
     4081 taagtactat taaattttga aaagaagttc tccttcagaa accttttttt cagagacttc
     4141 tagtttaata cccttatctt cgggaaatag aaccataaca acatctgaca gtttttccgc
     4201 cttgatttgg tcacaatcaa aaatcaccaa tgactcgccg gatgatactc gttgaccttc
     4261 ttcaacgagc acttgaaacc cgactccctt taactgaaca gtatcaattc caatatgaat
     4321 caataaatct ctaccttgat tactagtcaa tgtgagtgca tgctttgttg gaaatatcga
     4381 tttaatgaca ccgtcaatcg gtgctaccac cttagcgttt gttggttcaa cagcgaatcc
     4441 ttcgcccatc atcccttgcg aaaatacagg atcgcttact tggctaaggg gaatcagatt
     4501 tcctgatact ggagcaacta atttttcttt tcgactaaaa aatcccattg acttactcct
     4561 taatcttttg aaatgtccat cacaaggttt aaaagaacat ccgccccttt tttgacatcc
     4621 tcaaaatcag tccactcatc aggatgatgt gtaatgccac ctttggatgg aatataaatc
     4681 atatttgttg ggacaaagtc ggtcatcatc atcgcatcat gtccagtacc attgttaatg
     4741 acttgatagc tatagcccaa ctgatcgcaa atatgtttca tcttctcaac attaccttga
     4801 tccataacac ttggatttgt aggattactg tagttaattc gagccatttc ctccagctca
     4861 gttctaactc cagtttcccc ttgaatcact gataactcat tcaacaactt ttctttaatc
     4921 tcttgaggat tagttgtctt tgtatcaaag gttctgattt cgattaaagc ttcgacaaag
     4981 tcagggacaa attgatttga gttcggtttg attgtaagtt taccgacggt tccggcaatc
     5041 ccttcaccaa gattcttcat ccactgttca acaaaaagaa caaatttaga agcagcaact
     5101 aaagcatctt tccgtttatt cattggagca gtcgagtcct cagtttctcc ataaaaacgt
     5161 gctttaaaac ggccgatgcc aggaataaat tcaatcaatc cgatatcttt gccttcatct
     5221 tccaggactg gtccttgttc gatatgaagt tcaataaaat ttttgattgt ttttggatct
     5281 cgcttagcgg cctctaaatc tggttccact ccataagcac gaagagcttc caattttgtt
     5341 tgtccatggc gattttttac tgtttccaac tcactcatgg ttaatgttcc catcatagct
     5401 cgtgaatttg agacacccgt acttggccca aatgtctcgc cctcctctgc attcatcaga
     5461 attaggtcaa ttgggtaatc attttcaaaa ttattttctt ccagaatccg cattgtttcc
     5521 agtgcagtaa caacgccagc cactccgtca aatctgcctc cattaacaac tgaatcataa
     5581 tgagagccaa tcatcactga tggtgcatca gcctttttac cggctctacg accaaataat
     5641 gtagagaagc cgtcttccca aatctctaaa cccagttgtt ccatttgatt gatcaaataa
     5701 tttcttgctt cacgatcttc tttagagtaa gcagagcggg tcactccttc acctggagtt
     5761 gcattgtact cggctaaatt ttcgatatcc ttttttatac gctcaaaatt tgtcttcata
     5821 attaaatcct ccatttccta caagtgaatg ttagcaccca tagaaagcgg ctacaatata
     5881 atcaatgaag attgaatcga tattcgaaaa ctgaatattg cttcggttaa tgacttagaa
     5941 atcattatcg tagattccac cataagaact ttttctcgtc tcataacaca actcgagagt
     6001 ggattggttt aaaaaactct cgataaatgc gtccacttta ttgacacaga tccatggatc
     6061 gatcgcacct tgaaatttaa acccagaaat agtcccatca tcttctatac cgattaccag
     6121 cttacctcca tcggcatttg caaacgctac tatgtgtttt ataatatctc taggcttaat
     6181 ccgtgcactt tttctatcaa aatactgatc ttctttttca cttataaact tttgaatctc
     6241 ataatccatt taaaatttcc cttcttaaat ttttttatta tagagccgta tattcaattt
     6301 ccacaaaaaa ataatctaaa acaaaacaga caccttcatt ttgatatgtt ctcccacata
     6361 tgaatcggtc gctatacagc attacatata gtaattattc atactgcgaa ataaatagct
     6421 ccaaatcttt agctggcgta gttaagctta aattcgaaat gaaagtttca ttgactattc
     6481 cactttcatc aaatcccttc ttcaaccaat cagagttcca caacgtaaat cctttaggat
     6541 tagaaacttt cttacataat tttgcactat aggatttctc tcttgaatga attaaagaaa
     6601 gtctttgtaa ccaaatttca actatatccg tattaggagt attaacaaat tttcttaaga
     6661 ttttatccaa gtataagttg atttcttcta cagcaataaa ctcaaaaagc ttacctaaaa
     6721 tcacaataca agcctccaaa gttctaggat tcttttgcat tatattaaca agaatactaa
     6781 tcaattggta gatatcatct ggtcttttgt ttaaagtaga aactctatgt gtatagactt
     6841 cacttagcgc tttacttaaa ctcccgcaat ttggatattt gtctcctaaa agctttacct
     6901 gtagcaaatg cttttgaagg ctaagcttaa acttccactt attattactt ttctctgtaa
     6961 aacttgcatg tttaagatcc cagtaaattt tatcaggctt aattgcatct aaaattatat
     7021 tatccgataa gaatgtttta tttgaattta gcttcaaatt tagatccgat aatacttcag
     7081 ataacaatct gataatcact tcagcttgat cttttcgaac cgaaaaaatt ctgtaatcat
     7141 ctctgtaacg aagtattttg aactctttaa tctctgtttt ttctatcttg ttcagtaaca
     7201 ttttatcagc atacccaagt actatttcag ctacaaaatc catcaataca tttccttgag
     7261 gtataccgtt cgtttgtcca ttttgtaaat atcgtatttt agaatctatt aagtttccaa
     7321 cgccttgagt atcttttcta tgtgtcttag cccattcttt tgaatgtact gcccacggga
     7381 tagtatgtgt gtacattgag ctataacaat cggtaatatc tgaatgaatg cagtagtcat
     7441 actccaaagc ataactaatc tgtgcttgtt caaggttctc ccaccaattt aaaatagttt
     7501 ccgcagtatc gctctttgaa gaaatagatt ccacaggaag tgatatacat tcaattcgct
     7561 tgtcggcttg gaattcttca aacttatttt tgatgtgatt ccaattattt ttctgtgtaa
     7621 tcccgtttac taagtctaca tatactaatg gatgtaatat ttgaaggggt ctccaagcat
     7681 aattaccatc tttattagct aatattcgat gattaacatc aactgcctca gagagcgatc
     7741 tcttatgtct aactagttca gtaaatggtt tgttttttaa taaatccact gctttctcaa
     7801 tgcctactga taaatcaata tagttgggca acgatgtact aaaaaaactt gatgttttta
     7861 aaaagaattg ctttgcttct tgatgattca tctctaaaat actttttcta gtcctagtca
     7921 aattcaaaat ctcctagatc aattaagttg taatgtaaga ggatacccta aggttacaca
     7981 ccctatttac tattatacca gtattcattc gtgatacata agggtgtaac attaagaact
     8041 ctatctaaaa gactacaagt agtcaccttt aattgtgagt aattttcttt atccaatcct
     8101 tgtgcaacct tgtcgtcacc ccttgtatat ccccgtaccc cggatgtttc ggaatatcaa
     8161 aaatgtgctc aaattcctga ttttcatcag tctcccactc atgattcaac ggttcatgcc
     8221 actctataaa ccttgaccat aaaatgttct ttctataaaa ctcaatagga atttcaaaac
     8281 agtaaaaatt gtatttgcta aaatctgtaa aattatcttt acgagcatac tttaaatggc
     8341 aggtatctcg ccacaaccaa attaaatctc gatccaatgg taactgaatt cgtttcgtat
     8401 actcttcttc aagccaatga agtgcaccaa ctgcattcgg attatcgaaa aagacagata
     8461 attcagtata gcgtttcttt ggataaataa atccttctga atcaacagat tgcaaaaaat
     8521 ctactggttg aaccgtgtac actttcattt attcgccacc tttttttgac tacttatagt
     8581 catttaccaa tttgaaatct gcatactttt tcattcaatt gagcccagca ttaattgtac
     8641 acgagttttc ttcactaacg gatagtatca ggatatacta atactatcct gaatagcgaa
     8701 atgaggtagc caaaatgaga aaagtcgatg aatatatcaa aaagaaatcc caaaaaagta
     8761 aagtatggca aagaacattt gagttagagt ataagaaact ctcagatagc ctcaaaaagg
     8821 acacttataa tccaaatgaa caaagtaaag cagataacca ataaagatta ccctcccata
     8881 tttaacattg cttcacgaat cagtaatcaa tgactacaag tagtcattga agcaaatctt
     8941 cataagatac ctgtagaata tccctaatag ctctcaattg atccagctta atatgttgcc
     9001 ggccaccttc aatcttaacc aatgtttctc ttgtcatagc gattttactg agctggagtt
     9061 ctcgcaccag ttctgtctgt cccattccct tttctaatcg gattcgtctg atattgctac
     9121 cgattacatt ttctttatct attaactttt cttccttcaa aaatagcacg ccttttggac
     9181 taaaattagt cctttttctt attaatctta aagatttggt atgctacaat gggactagta
     9241 ttagtccata ttcaatttta aggaggaatt tttttgaaga aatttgcaat cattctaacc
     9301 tgtgctttac tcacgggagc tataagtggg tgcgccactt ctgacacaaa ttctacaagt
     9361 actgaaacaa gtacaacaac ctcagaaaag aattatccta tacatttttt tccagttcca
     9421 gatggatttg aagcttatta tcatgataaa aaactagatg ttccattgtc agaaagtaac
     9481 tctattgaaa aagttttgcc aattgaaatt gaaaaaaatc aagagaatat aaaactcaag
     9541 gccgagcttc ccatactgaa cagcacagaa attgattggc acgagaatat taaagtaact
     9601 attgaaaaag gagcgttagc acaactttat actgaaaaag ttgaacaatt ttttgagtct
     9661 ggtgccaata aagacttttc aaaaataagc gattattcgg ataacttttt taatgagacc
     9721 caagatgaaa tcacgaactc aacttttatg gctgaagata tgtacaatat gacgttaaaa
     9781 aaaataaaag cttacgacca tccattaaat ctgacactaa ataatggatt actctctttt
     9841 gtattagcag gttacgttga ctatgaatat tcaccagatt atttggagac acctcttcaa
     9901 cacgagaaca atgatattaa ttttagattt gtctacgagt caaaaagtaa aacatggtta
     9961 gttgatcaat taagcactat aaacacaagc ttccatgaat tacctaaacc tgaaaatgaa
    10021 gctgatatgg tcattaaaat cttctaaaaa agaagcgttg aaggagaaat attcctttca
    10081 acgctctttt ttatatgctt agaaacataa ataaattgta tactttcttt ttagccccta
    10141 ttacttttca cgaaaaatat cttttttcga cactagcgtt tctctactta taatattgta
    10201 cacagttttt gtattattga aaaattactc attatttagc attaatccac atcgttttat
    10261 caatcgacct tcatactaaa ctactttaaa gtcatcttcc ttctcatttt tcattaaatc
    10321 agatacataa gcccatgtac ccgttcgttg taaataaagt tcttcttgta gcagctccca
    10381 ctttctctta gaaatcataa tgacgccatc gttcaatcca tttttagaat ccctagtaat
    10441 ttctatttca gaatttgaat gaactacttc ttgcaacact ttacctatat tttttttgaa
    10501 gtttgttgga gtaatagtat ccaaaatgaa cacctctctt ctctatacct ctattatgaa
    10561 gcgcccatca aataaagcaa gcgttgagga ataattcttt caacgcttgc tttattaagc
    10621 tttcattttg tagtttgact aataccaata atctgatctc taaacaatcg aaaaacttct
    10681 tgtggttgat aataatctgt atctatttca aatgtttctg agaacaattc attgaatttt
    10741 tctactgaaa tgatgatttt accattctcc tctcgactgt tttctattat ttctgcttct
    10801 aattcaaata aatctagtga cgaactaaaa tcataaatcc tgtcatattg aacaaaaaaa
    10861 tcaagaaaaa gttgctcaaa ctcttcctta tttatcacta gtgtttcaag cggaacaact
    10921 ttattttcat cagccattct tccaatttcc tcactttcat tttcaaaaaa tcgtaaaatt
    10981 gatatagatt ctttgtctct aatcactcgt gatattatct tgacagtcct acatctaatt
    11041 caattaaagt ataagccact ttcaaataaa agattacaaa gtaaatggca agccaacaaa
    11101 ataaccatct gcttcacgta ttacgatttg ataattctgg atttcgctcg gaagttcata
    11161 ataataatta acagtttctg ctttgccact ttcaatatat tctggattat tcaatatcat
    11221 agcatactca taaagttctt caccattttt atttggttct ataatatttt gtgttggtgg
    11281 aaaatcgaag gcgattttct actaacactt tttcttcgtt t
//
LOCUS       DACEQH010000076        10795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.078, whole genome shotgun sequence.
ACCESSION   DACEQH010000076 DACEQH010000000
VERSION     DACEQH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.078"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..407
                     /locus_tag="IW833_002358"
                     /pseudo
     CDS             1..407
                     /locus_tag="IW833_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(309..1025)
                     /locus_tag="IW833_002359"
     CDS             complement(309..1025)
                     /locus_tag="IW833_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ1308447.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1149..1334)
                     /locus_tag="IW833_002360"
     CDS             complement(1149..1334)
                     /locus_tag="IW833_002360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308448.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            1561..2834
                     /locus_tag="IW833_002361"
                     /pseudo
     CDS             1561..2834
                     /locus_tag="IW833_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2941..3972)
                     /locus_tag="IW833_002362"
     CDS             complement(2941..3972)
                     /locus_tag="IW833_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1308449.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3979..4809)
                     /locus_tag="IW833_002363"
     CDS             complement(3979..4809)
                     /locus_tag="IW833_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1308450.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4806..5612)
                     /locus_tag="IW833_002364"
     CDS             complement(4806..5612)
                     /locus_tag="IW833_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1308451.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5618..6442)
                     /locus_tag="IW833_002365"
     CDS             complement(5618..6442)
                     /locus_tag="IW833_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ1308452.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6432..7532)
                     /locus_tag="IW833_002366"
     CDS             complement(6432..7532)
                     /locus_tag="IW833_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1308453.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7710..8495
                     /locus_tag="IW833_002367"
     CDS             7710..8495
                     /locus_tag="IW833_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ1308454.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8528..8911
                     /locus_tag="IW833_002368"
     CDS             8528..8911
                     /locus_tag="IW833_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1308455.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            8987..9118
                     /locus_tag="IW833_002369"
     CDS             8987..9118
                     /locus_tag="IW833_002369"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1308456.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9087..9323
                     /locus_tag="IW833_002370"
     CDS             9087..9323
                     /locus_tag="IW833_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1308457.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9401..10372)
                     /locus_tag="IW833_002371"
     CDS             complement(9401..10372)
                     /locus_tag="IW833_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ1308458.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3207 a         2514 c         2077 g         2997 t
ORIGIN      
        1 agattggatg actcaccttc ccattgtgaa cactgtggat cgattcgtat tatccgcaac
       61 ggttcctata cgacacgtac tcagatgttg aaagtcaaag aaaagttgac tattttagaa
      121 ttaaaacgta cccgttttct atgctatgat tgtgggcaga ccttttctgc gaaaacggac
      181 ttagttgatg aacatcatca actaacaaag gaactgaagc aggcgattct catagaactg
      241 tacgaaaatc aatcacgtaa tctgaaaatc agaagaagta ccgtcggctc aagcgatatt
      301 ggaaactctt actcaaagat tccacgacat tggagccgct caaacggcat tatcatcgac
      361 tgtttaaacg accaattagt caaacagaaa tcgtcgatga accgttgtgc acaatggtgt
      421 cctgaacgta cagcgacacc ttccatatcc agagcagtcg ccacatcacg gggatggatg
      481 ccatccaaat taaaagcaac cacgccagta tgatgttcag gatcttgcgg tccgtaaatg
      541 gtgataccgc cgatcgcttg taatttaggt aaaacgtagg caaccagttc ttgctcataa
      601 cgatgaatat tctccatacc aatctcattc aagtaatcga ttgctcctgc aatgttcgga
      661 gtaccagctt caaatttcca tggcagctct ttccatgaac tatcgtaaag attgacaaaa
      721 tctatcattt caccgccgaa ttcgactggc tccatttgtt ctaacaattg acgtttgcca
      781 tataatacac cgattcctgt cggcccgcac attttatgcc cgctaaaggc gaaaaaatct
      841 gcatccaacg cttgtacatc aactgtcata tgcggtgctg cttgggcacc gactggacta
      901 tcctcaacta caacagcctg ttcaaccctc agccgtttca agcacaacaa ataaccttct
      961 ggatctggtt tgccgacctg aagatcatcg atcgtcacaa aggaatcaat tttttcctta
     1021 atcatcggat gtgcttcaaa aaaatagtct agctcccgca gttcagaatt gagactactc
     1081 cgattttcca gtgctgctga tccgctgctc aaaatgttca gcatccactg gtctcccaac
     1141 cgcttgactt atcccttcat aaaaggacag ttctgctgcc gtcccaacac cttgttgata
     1201 gtctgcaaaa ggaattttat agccgaactg ctccagtaaa taggcttcag taaatagtga
     1261 tgaatactct cgtaaaacat ccattcactg tcaatcatcg tcccatcaaa atcaaaaata
     1321 agtgctttgg tcatcttctt tcccccttac ccaaaacaag caaaagctgt gtcctgctct
     1381 ctctacagta tcagcataaa aactgccgtc tgaaaagtca aaggtgaaag aaatgtggcg
     1441 ctataatttc taggactgat agtaaatcat tctatcagtc cttttgagca taaaaaaaca
     1501 aaacatacag aagctccgtt agaattgaat caccacaaca caatcaaagg agcgttctgt
     1561 atgcctgatc ttcatcttat caaactatta ctaggaatca aagatggaaa cattcaaatc
     1621 actcatgtcg agaagaaaaa aatacattca atcaacagta tcgttgtttc tgccacgctc
     1681 attaaaacga ttcagccctg tcctcagtgt aaacaggtga atcaacatgg gctgatcgtt
     1741 aagaacggaa agaaaaaatc gctggtcctt ctcaataagt gtgcgaatca acggacttat
     1801 cttgctttgg caaagcaacg gtacctctgt cgtggctgtc acacctattt tacggcgaat
     1861 acctacattg ttgaaaaaaa ctgttttatc gccaaacaag tacattataa aatattggag
     1921 gaattgactg aaaaacaagc aatgacgacg atttctaaac attgcggcgt ttcttggtca
     1981 accgtttcgc gaacactcgc ttctctgctc cccatgacaa acgtgaaaat aaattggttg
     2041 cctcgctgtt cattggtaga tgaatttcgt tcattgaaaa atcaagtcgg tccctattct
     2101 ttttcttgta tggatggcga tactgggaaa ttactggata ttcttccttc acgtaagaag
     2161 aaagatctca tttcttattt tatgcaattt gaacgacgtg cacgtttaaa cgtaaaaata
     2221 ctcgtaacag atatgaacgc ctcttatgct ttgctgataa aagagtgctt tcctcatgcc
     2281 aaactcgttg tggatcgctt tcacatcgtc aagcacctca ttcgcagttt taaggatatt
     2341 tgactatgag tcatgaaaag cttcgaccga aacgatccaa tacaagccaa acactatcga
     2401 caagtgaagg ctttatctcg acttctgatc aaacgacagg acacgcttgt ttatgataaa
     2461 tggacaaaat ggcgtaattt tggttgggcc tatttaaccg aaagtgaagt cgtagaacgt
     2521 ctactttcta cttctgatga actacgaata gcttatgctt actatcaaga aattcttcaa
     2581 gctttttatg acaaagaagc ggacatcttc tttcagcttg taaaaacaat gcccaagtcg
     2641 gttccaagag aactgcatca tataaaaaaa gcgtttataa actatgaatc cggcattcgt
     2701 ttagccttag agttgcctta ctcaaatggc aaaatagaaa atctgcatac ccacatcaaa
     2761 gcattgaaac gaatagctta cgggttagga tgagaaccag aattttctta atcaatcatc
     2821 tgatcactta ttaaacataa aaaaacatct aaagcagaaa atcaattcta ccttagatgt
     2881 tcttatatca ctctatcagt cctatttgac aaagagccag aaatgtttgc cgactataca
     2941 ctagctttgt tgtgcaaaat gctgagtatc aatttccaca gggattgcta gaatatcttt
     3001 cagctgaatg tataaggcat cgctaatttt ctcttcccga tcgttcaacc gatccactaa
     3061 aatttctttg ccctccagcg tttgaaaaac ataccgttga atgttgccta aaataaatga
     3121 ttccttccat agaaccggaa tcttaacccc ctgaggaacc ggttcaaatt gaatcagctc
     3181 cggtcttaaa taataaatgc agcgattgcc cttagcggaa aactcgtggg tatactgatt
     3241 cagctcatac cattcgaaac gattatagtt gccgataaat tccgcaacaa atcggctctg
     3301 aggattctga tacaattcct tcgaggtgga aacctgagcc acccgcccag cttccatcac
     3361 aaaaatccga tcactgattc gcatcgcctc ttcttggtca tgagtcacaa aaatcatcgt
     3421 catccctagc gattgctgga tagcgcgcaa atcccgctgc agctgttttc taattcgggc
     3481 atccaaagca cttaatggct catccagcag caggactttc ggttcagtga ccaatgagcg
     3541 agccagtgcc acccgttgtc gttgaccgcc agaaagaaac tggacatttt tatctgcctg
     3601 ctccgtcata tccaccaatg atagcatctc aaaaaccttt tcctgaatca cctcaggact
     3661 cagcttcttt accttcaaac caaaggctac attttcaaaa acagtcatag ttggaaacaa
     3721 tgcatactgt tgaaaaacaa agccgatatt gcgatccttc gtaggcttat cggcaatatt
     3781 ctcattgtcc agcacaatct caccgctctt tggttgttcc aatcccgtga tacagcgcag
     3841 caacgtagac tttccgcacc cgctgggacc tagtaaagta accagctcac ccttttcaat
     3901 ctgaatttca tcaatagcta aaatctgttt ttcatcaaaa aacagattga gatcatgtat
     3961 ttctaaaaac attttccgtc acaactcctt taagggcttc tttttcgact gcttctcttc
     4021 cctcgtctgc ttaagggaca gctgttgctt ttgtcgataa gtcagcataa aggtagttaa
     4081 agcaattccc accataaaca gcaaatacaa aaccatcaca gcgctagcca aatggccgtt
     4141 ttcgttcatc cgccgcagca tatagatgcg taccgtctga aatcgaccgc caatcaacag
     4201 gttcgtcagc atgtactccc caaaaaccga agaaaaaatc atcagagaaa ctagggtcac
     4261 accgatacgg atgtttggaa acagcacctg gataataatc gtagacatcg gggcgcccaa
     4321 ggtttctgct gcatccacta attccttcag attaattaag cgcatctggt tgttaagact
     4381 aagatagaca atcggcaacg ccgtaataaa caatgcacct accaacacta caaacattgg
     4441 gataccagtt tcagcatacg ttcgaagcaa agccgttacc aaaatcactc ctggtaaagc
     4501 atacggcagc aggagcagct tttccagcca acgattcaaa cgagggaaat acaaatgaat
     4561 ccaaataatg gtgggaatca tcaacaacag aatcgtcacc aagaccacac ctgctaataa
     4621 aagtgagcga cccaccgcag caataaattc cctgtccgtc agcagctgtt ggaaccattg
     4681 caaggtaaaa tcactaggta aaagcgattt tgaccattta gtggagctgg catacaaaat
     4741 cgttccaata atcggcagca agtaatacag gacaaagcag aacaaaatcg gttttattaa
     4801 ctttttcacg tctgcaccac cttgcctttc ttttgaataa tcaacattgc cgttaccagc
     4861 aggacaccta aagcgactgc caacgcactg cctaaattag gcttggcgaa cacatctccc
     4921 gacactaaag ccgcaattcg aatcgttaaa agatttacat tactgctggt cagggcatac
     4981 gctgtttcat aggtccccat gccattagca aataagatga caaaggtgct ggcaatcgat
     5041 ggtcgcaaaa aaggcaaagc caccttgaac caagcatacg ctgatgaagc gcccagcaat
     5101 tgtgcggact ccagccattc ctttttcact tccttcatgc tgggatacaa aaacaagacc
     5161 cctaacggaa tttggaaata ggtatacgtg atggtcaatc cccaccaaga atacaaattg
     5221 aagtccgcta gaaatggcaa aatcattttc agcacaccta cattccccaa caaaataata
     5281 aaagagaatg ccagcggaat cccagcaaaa ttagctgcca aattggaaac aaaggtgatt
     5341 ttctcctgca atttagtcga tagattcatt aagcataaac ataaaataaa cgtcccgatt
     5401 aacccaatca cactggagct gacagcaatc agtacgctgt taacaaaggc ctgtgtgtaa
     5461 tatttgttgc tgaaaatatc actgtaattg gccaatgtcc actggttagt taaatcactg
     5521 tgcaagctgc acagaaccat aaaaagcagc ggcagcagta aaaatgcaca gaagataaag
     5581 gaggcaggaa ccagaaccaa gcgttttttc atctctttca tcccctcatc aattcttgaa
     5641 tgtcctgcat tttctctgac ggtgcaattc ctaataacca gcaaaacaat ggagccgcca
     5701 gcagctgtgg tacagtttgt tccttcagct tttggcgttt atttgaaatc aaataaaaag
     5761 gaacttcacg atgtgcagcc aagctgccgc catgcagtcc gtgcgcatcc atcccgtgat
     5821 ccgcagtcac cacgacttga taaccttttt gcagccattc aggaaggtaa atagccaata
     5881 atctgtccgc ctgattaacc gctgctgcat attcatgaga atcggcggta aatttgtgac
     5941 cgacatcatc aatattcatc gaatgtacga ccaaaaagtc tggctgatac tgattcacta
     6001 aataatgggc atccgcaaat aaatgcgaat cagggtaagt gtcctcacta taaaagatgc
     6061 catgctgaat cagtcgctcc tcctgatgct ggatgcgatc aaccaaacga ttataggggg
     6121 cttgattgta caactcgctg taccagtggt aagcggcggc tcctgttttt ccgccagctt
     6181 ccttaactaa ttcaaacagc gaccactctt tggatcgctg agcatggtaa ttactataga
     6241 tgccattttc ataggctgga acgccggtca tcagtacttc gtataacgga cgtgaattgc
     6301 tgggcagctc actttttacc ttcaataagc ttgcttgctg gtgctcaact aaatgattca
     6361 tgaacccaag ctgggcgacg gctgtgtcgt agcgacagcc gtctagtata actacaatca
     6421 gtttgtcaag tctactttgc ataggtcagt acctccgcct gccaaagctc tggaagctct
     6481 acagaggtct tgttccaagc ttcattgtct tcaactggca ccgccgcttc ataagcatcc
     6541 tccggcagta atttttcagc tacttcttta ggcagctcta ccttgcgaat aggtcgcgca
     6601 tagccctttg ccaaattgat ttgcccttca tcagataaaa tatattcccg tgctagcttt
     6661 gccgcattgg gatgaggggc atttttattg attaaggttg tatacccgct aatgatggag
     6721 ccgtctgttg gcacagttac ctcaaaacgc tcggagtcaa tttggtcacg ataatttagt
     6781 ccattgaagt cccaaacaac tgccacatcg atttcacctt tttcaagatt tgctacgctg
     6841 gcatcaacgg tggacaaacg gcctttttct gccatttttt tgtagtaatt cagacctggc
     6901 tgaatatcct tttcgtcacc tccgttggca atcgccgcag ccaatacagc aaattgtgct
     6961 tggttggcaa tcgtcacatc ccccacgcta acttgatagt caccctcttg cagctccttc
     7021 cagctagtcg gtgcttgctt cacattctct ttatcggtga taaaggaaat ggttcccgta
     7081 tagcttaaca accaatcccc atcatcatcc tttgcccaat ccggaatttc ctcccaatag
     7141 ttggtcttat aaggtaaggt taactctttg ctttcagcca acggaccgaa gctgataccg
     7201 acatcaccaa tatcggcggt tccatttttt ccttctgatt caaacttagc caattcttct
     7261 gcactggaca tatccgtatc cacgtgactt aagccgtatt ctttttcaag gtcgttccaa
     7321 gtgcctaccc aatttgccca agtatccggc attcccaagc tggcaatctc tccttcttcc
     7381 ttggcttttt ctataatcgt atctaaagag tcgtctgccg cagccgattg cttcgttgct
     7441 tcacctgaga cgcagcctgt cagcaggata cttactgttg ctgcacaaaa taaaccgata
     7501 gtcatgttac gaattttttc attcattttc attcattctt ctcccctttc gataattcca
     7561 ttctaacaaa gctttgtaat gagagaatca gtttcttgaa aaggtttcat gaacaatgta
     7621 aaggttgtat atggtttgta aatttcgacg aattcccgtt aaccttctaa agcttttgcg
     7681 gtatgatagt agaaaaaagg aggctcccta tgcagtatta tgatcagcat ctccacacgt
     7741 atttttcctt tgattcagag gaaaaatttg aaaactattt agcctatcag ccggagtttt
     7801 tcgtttctac ggatcacttt gatttaaaca atccatctac cggaaaagat gacattccgg
     7861 attatccagc ctatataaac aaactggact ctttaaaaga aacctattcc accaaatttc
     7921 tgcgaggtat cgagattggc gttgttccag gacaagaaga gcggatcgtg gattacctcg
     7981 ctcagcatcc ttatgattta aagctggtaa gtattcatca aaatggccgc tttgattata
     8041 tggacgatgt ggttttaacc aaagatccca ttcaaactac caaggaatat tttgatcaga
     8101 tgttgcaggt cttgacggat tttccagaag gagatatttt gactcacttt gattatggat
     8161 tacgccgctt caccttcaca gcggaagaat tcgcggagca ttttgaggct cagctgctaa
     8221 cgattttcaa aaaggttgtt gaacgagaat tggcgatgga gttaaatgcc aagagcttct
     8281 tgaaatacgg caacgaagac ctttaccgtt atgccgttcc tttgtatcaa agtgtcggcg
     8341 gaaagctctt taccttaggc tcggatgctc atgttgccga agattatcag ctgggcttta
     8401 aaaaaatgag tcagctgttg gcagactgtc aggtagaaaa gttgctagtc attcaaggaa
     8461 aagaacgccg cctgacgccg ctgccgattt tgtgattcct atataataga agaaagtggt
     8521 gaaaaaaatg agcaacctgt gggactacaa tcaagaggca cccatccatt atttaattgc
     8581 gcgacattgg gatgcgttga aaattgaggc ggtttgccga tccttgttag cagccgttcc
     8641 aaaacaacaa ctggaaaatt ttttggtggc tgacagcttg cagcgggaaa aagttcaggc
     8701 ttattttgca gcctttaaag atcagccttt ggaatatttg catgcgcagt ttcatctttt
     8761 ttatcaagta gccgccccag atgactacaa cgatttacga ggacagctgc aattgacctt
     8821 tcaagcggat gaaaccgcct ataccgtttt attgggaatg gctcgccttg gtgatcaggc
     8881 gaaggtcgag tggcggattt tcgatatttg atggcaggat caaggcgttt cagttgcaaa
     8941 ccttcctctc tttcaattag aaaaaccgca gcaggaagaa gtttgtgtgc tggtcaccga
     9001 tgctggtgaa attcgtctcc gcttatttcc tgaccaagct cccctggctg tagccaactg
     9061 gcgctcccta gctgagcaag gcgtttatga caacacacct ttcggctcgt gtcattaagg
     9121 aatttgttat tcaaggtggc gctttggatg gttctggtga tgaagctgtt tccagctatg
     9181 agggcttttt cgctgatgaa gtgcatcgcg gactttatca ttttaatggc gccttggcct
     9241 taggggacca cggcccccac accaacggca accagttttt tattgtgcaa aacacaaagg
     9301 cgcaggctga cctgctaatg taaagcaagc atacttggag cttggaggac taccggagct
     9361 ggatggtcgt tatactgttt ttggacaggt ataccaaggg ttagaaatcg tagcagcgat
     9421 cgcagctcag ccgacaaaca gtgaagatgt accgattgag ccagtgttca ttcgacaaat
     9481 taacttcgaa aaaacatcgt aaaaagacac tcactgactt cagcaaaagc tctattcttg
     9541 agctggctgc agctagtgag tgtctttcat ttatccataa gataaagctt ggattcaata
     9601 aataaataaa tgttttggtt aaagctgtga ccagcaaatg aggaaccgtg cgagccagtt
     9661 gatctgccaa tgcaaacatt tctttaggtg aaggcgctgg cgtgtctgcc aacggatact
     9721 ccagtcccaa tgcttggtaa ccgtgcatac catagcagtg gtacggtagt aattctaacg
     9781 gtacgaaccg atcaagatct gccacaaaac aagcaatcgt cagcagctcg gtcaggttca
     9841 tattgatacc tggaaccaca ggaactcgaa ttcgcacttc gcagttagga tactcaatta
     9901 aaaagcgacg aatattcttc aaaatttgtt ggttatctaa gccggtccat tgtttgtgga
     9961 gcttgctggt aaacattttt acatccacaa acaatgtatc cacataggga gctacctttt
    10021 ggatttcatt ataagcacca caaaagctgg tttccagtac ggtattgatt ccaatgtatt
    10081 tcgatttttg caaaatttct ttagcaaaat ccgcctgaac cagtgcctcg cctccgctaa
    10141 tggtcactcc gccatccgag tgaaagtaaa aaacagcatc cttctcaatc tcatccatta
    10201 cctcttcagc ggtcatggtc tggccaaatc ctttttcaat ctgcttgctt tgtgattcgg
    10261 gaatcgaaca ccaggcacag cgtaatgggc agccctttaa ataaacaacg gtccgtaaac
    10321 cagcaccatc atgtagagaa ccctgttcta ttcttaatac tgttccttcc atatagattt
    10381 ttgaattaca cactagttta aactaaaaca cacaaggaaa aacttaccat ttaatcataa
    10441 aattagctca tataaatata ctaggatgta aaatagactg gtcaaaatct ggtcaaaaaa
    10501 gaggactaat acaggtagtc ctcttttttg ttattttaat attctaaatc attttttatt
    10561 ggtttattcc cttattcgat gtagactcaa atcactatat cactagatat caaacggcgt
    10621 catacggaat caaaaacatc aaatttgacc tgttaaatgg tcattgaata acattcaaaa
    10681 tcaataaaac ggagtcagac acggagtcaa aaacgctgag agtaaaaaat atatacagcc
    10741 ttgtaggtaa agtaccacag ggctgtatat tttttatttc agattacctt aaatg
//
LOCUS       DACEQH010000077        10437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.079, whole genome shotgun sequence.
ACCESSION   DACEQH010000077 DACEQH010000000
VERSION     DACEQH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.079"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            91..309
                     /locus_tag="IW833_002372"
     CDS             91..309
                     /locus_tag="IW833_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308459.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(598..1110)
                     /locus_tag="IW833_002373"
     CDS             complement(598..1110)
                     /locus_tag="IW833_002373"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ1308460.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1124..3421)
                     /gene="recJ"
                     /locus_tag="IW833_002374"
     CDS             complement(1124..3421)
                     /gene="recJ"
                     /locus_tag="IW833_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ1308461.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3563..3997)
                     /locus_tag="IW833_002375"
     CDS             complement(3563..3997)
                     /locus_tag="IW833_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ1308462.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4007..4795)
                     /locus_tag="IW833_002376"
     CDS             complement(4007..4795)
                     /locus_tag="IW833_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1308463.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4792..5733)
                     /locus_tag="IW833_002377"
     CDS             complement(4792..5733)
                     /locus_tag="IW833_002377"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ1308464.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5910..6497)
                     /locus_tag="IW833_002378"
     CDS             complement(5910..6497)
                     /locus_tag="IW833_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ1308465.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6718..8031)
                     /gene="obgE"
                     /locus_tag="IW833_002379"
     CDS             complement(6718..8031)
                     /gene="obgE"
                     /locus_tag="IW833_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ1308466.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8319..9329
                     /gene="gap"
                     /locus_tag="IW833_002380"
     CDS             8319..9329
                     /gene="gap"
                     /locus_tag="IW833_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1308467.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9414..10349)
                     /locus_tag="IW833_002381"
     CDS             complement(9414..10349)
                     /locus_tag="IW833_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1308468.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2888 a         2356 c         1809 g         3384 t
ORIGIN      
        1 ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata tacgaacaat
       61 tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct tggcttagta
      121 ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc agctttactt
      181 gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa gtaccgtcaa
      241 tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta cagatccagg
      301 caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa cttttacggg
      361 ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta aagccagttt
      421 ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg gtctttgctt
      481 gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct gacttttgag
      541 ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt tttctgctta
      601 atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat ggagatccat
      661 caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga tcagttcgat
      721 cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca cacgttgacc
      781 tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta atccataagt
      841 cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa aaccgactcc
      901 taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc caactaccat
      961 atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc gataagcgtc
     1021 accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg gatagtcggg
     1081 aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt gttaaccatt
     1141 tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt ttattgagcc
     1201 gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg actttttgta
     1261 aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc ataaaaatta
     1321 ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga cgtatatcaa
     1381 ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt gtgcctaccc
     1441 cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg aatgaataag
     1501 accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca actagagagt
     1561 ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa acaatcgcag
     1621 cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct aaataaaggc
     1681 taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca aatacttgca
     1741 gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg ttccattcat
     1801 tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct tcttgagtac
     1861 caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca aatttcagat
     1921 gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga aataaaaact
     1981 gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca attcgctcta
     2041 ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct ctcatcaagt
     2101 caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg acattttcac
     2161 ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat aaatctcgca
     2221 tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat cctttagctg
     2281 atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc attattttcc
     2341 cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga tggatatgat
     2401 tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt gcttttcttt
     2461 cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca tcaaacgtcg
     2521 caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt agtctgggag
     2581 caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg ctttcttcaa
     2641 atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct aatgaaacaa
     2701 ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc gctactaaat
     2761 ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat gctactccga
     2821 ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga acaatcgcat
     2881 aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg tctacaccag
     2941 cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca gtgatgatca
     3001 actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca tacccatgct
     3061 caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt gtttctttca
     3121 ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg attttttctc
     3181 cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt atcagatacg
     3241 gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt tcctcatggt
     3301 aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca cggatcattt
     3361 ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt gcttgtctca
     3421 ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa agcagaaaga
     3481 aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga tcttttgtac
     3541 gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg ggcgattgag
     3601 ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct tgtaactccg
     3661 cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc tgttcttctt
     3721 ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc tgagtcagct
     3781 gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat acaatcagtg
     3841 caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag gaagtgaagc
     3901 caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata atcaaaacta
     3961 aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac tttttgttaa
     4021 aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat tttgatgcaa
     4081 cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg cggacgatca
     4141 tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa tagcttccgc
     4201 ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact gtcgtaacag
     4261 ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg actgcaaatt
     4321 tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata gaggcgatat
     4381 taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt gtcaaaacca
     4441 tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg aatgtaagga
     4501 agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc ccgacttctt
     4561 ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct accttgaaag
     4621 cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct acaaatgcca
     4681 ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa cagatttgtg
     4741 cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa ttcatgcctc
     4801 ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg tcgtgttttc
     4861 aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat atctcgcact
     4921 gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag cagcttgatg
     4981 agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa atgtgctttc
     5041 atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt ttctcgtatc
     5101 gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt ttccattgat
     5161 cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt acagtggtcc
     5221 agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat gatcatgttc
     5281 aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat aaacagaaaa
     5341 ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca gcggataact
     5401 taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc cttttggccc
     5461 gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga gcccgggaag
     5521 tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt ttctcggacg
     5581 aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca accaaacttc
     5641 attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt ttgcaggaac
     5701 acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct ttaagttcct
     5761 ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg ggttcaaatt
     5821 aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa acagcagcga
     5881 acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact ttcattgtcg
     5941 ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt cgcatgattt
     6001 tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa gcgagtaatt
     6061 tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc ttcatcagca
     6121 atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc gaacacggtc
     6181 tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc ggcaaaccat
     6241 cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat taatccaccc
     6301 gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa ttgatgtgtc
     6361 ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg aacagcatgt
     6421 aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa atttaaaatg
     6481 attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt attttacctt
     6541 taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg gctgtgacaa
     6601 aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga tcacttctgt
     6661 cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt tgcttgccta
     6721 ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt tcgcaccgcg
     6781 tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac gcatcactgt
     6841 ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac cagataagat
     6901 ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat tgaatccata
     6961 gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg ttacttctaa
     7021 caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc cagaaattgg
     7081 aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct gttctttgaa
     7141 tttcactaga ttttccggtg catccggcat atccattttg tttgccacga tgatttgcgg
     7201 acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa gatagtcttc
     7261 ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta ctcgtgttct
     7321 ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc cttcaatcaa
     7381 gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca ttcctagatt
     7441 aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg aaataacgga
     7501 tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg ccaatacttt
     7561 caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa tttccggtgc
     7621 aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac cttttgccac
     7681 aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct ccgcatctcg
     7741 gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac cgtgcatccc
     7801 tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc gaaaatccat
     7861 caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac caccgtctcc
     7921 gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca ttccgtctcc
     7981 gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca taattaagtc
     8041 ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca aaagtcttac
     8101 ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt gtttaccttc
     8161 ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag tataattttt
     8221 tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa ttgaggatgc
     8281 cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa gtaggtatta
     8341 acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc tcagatgata
     8401 ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa ttattgcaat
     8461 ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac tcaatcgtag
     8521 tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc ccttgggcaa
     8581 aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct gaagaaaaag
     8641 caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca gctggtgcta
     8701 tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac aaaatcatct
     8761 cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta aataatgaat
     8821 ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca caaatgctct
     8881 tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct gacaatacta
     8941 tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag ctacaaggaa
     9001 aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact gaattggttt
     9061 cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa aaacacacag
     9121 ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat gtcattggaa
     9181 caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct ggtgatttcc
     9241 aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc caaatgattc
     9301 gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa agaagccttt
     9361 gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact cgttcaatga
     9421 acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc tgtcttgtat
     9481 gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt cgttgcacga
     9541 tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg tataaagagc
     9601 attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa aaagtagcca
     9661 gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg aagactaaac
     9721 atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca atcttcaatg
     9781 attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt tgctgctccc
     9841 atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag cactttgttt
     9901 atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc cccttcgctt
     9961 tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg aaacatcttt
    10021 gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag agctgttttt
    10081 tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc ttttgaagtg
    10141 atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc cgtttctttg
    10201 gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga aggcacaaca
    10261 gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc taaagcgacg
    10321 cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc ttttttcata
    10381 taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt ttataac
//
LOCUS       DACEQH010000078        10296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.080, whole genome shotgun sequence.
ACCESSION   DACEQH010000078 DACEQH010000000
VERSION     DACEQH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.080"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            294..1481
                     /locus_tag="IW833_002382"
     CDS             294..1481
                     /locus_tag="IW833_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1308469.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            1659..2966
                     /locus_tag="IW833_002383"
     CDS             1659..2966
                     /locus_tag="IW833_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ1308470.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFDNLF"
     gene            3085..4284
                     /locus_tag="IW833_002384"
     CDS             3085..4284
                     /locus_tag="IW833_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1308471.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4311..5579)
                     /locus_tag="IW833_002385"
     CDS             complement(4311..5579)
                     /locus_tag="IW833_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ1308472.1"
                     /translation="MEKQLPSPMRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLITLTL
                     FGTITTHLFGGSVGREGTAVQMGGSLADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(5742..6389)
                     /locus_tag="IW833_002386"
     CDS             complement(5742..6389)
                     /locus_tag="IW833_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAQ1308473.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILNHELPEIDTFLTTVLDVKLTKAQAERALEKLKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(6797..7300)
                     /locus_tag="IW833_002387"
     CDS             complement(6797..7300)
                     /locus_tag="IW833_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1308474.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7423..8187)
                     /locus_tag="IW833_002388"
     CDS             complement(7423..8187)
                     /locus_tag="IW833_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ1308475.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSADQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8294..8569
                     /locus_tag="IW833_002389"
     CDS             8294..8569
                     /locus_tag="IW833_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ1308476.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9285..10235
                     /gene="yidC"
                     /locus_tag="IW833_002390"
     CDS             9285..10235
                     /gene="yidC"
                     /locus_tag="IW833_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1308477.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         3122 a         2087 c         1942 g         3145 t
ORIGIN      
        1 ggttttaacc ttccggtttt gattggataa ccctatgaac catcgttatt ttaatttggt
       61 attaactcat ttcttttgag ataatatatt tattggattg agaggctaag ccgttccccc
      121 cttgggccat tgaacccgca ctcgagagac tggctcctct ctctgataag tggattgcgt
      181 atgagttaga tgttgaaagt ccttacttta ctccgtatct cgtacaaggt agtgacgctt
      241 ttcagttgag tggatttcaa tccaaaattc tttttttgaa aggtcagata tacatgtact
      301 ttttaggcat tgatatcggt aaacgcactc acgttgcttc tatcatgaac gaggaaggaa
      361 aggtattact aaaaggtttt tcatttccta acaccactga aggagcagag tcattaattg
      421 agcgaatggt cgactattcc ggagcaccca gtgactttgt tatagggatg gaagccacgg
      481 ggcactattg gctgtccatc ttttcatacc ttcacgaatc agattatttg attcacgttg
      541 tgaatcccct tcaaacagac ggatggcgta aaggtacaga aatacggaaa cgaaaaaacg
      601 atattattga ttctgttttg attgctgact taatgcgcta tggctcattt gtcgaaacgg
      661 tcttatccga tgaaaatgtt ttttctttga aacaactttc tcgttaccga acttatttag
      721 taggtacagc gagcgacttc aaaagaaaaa ttatagccgt attggatcaa gtctttccag
      781 aatacgcgac gatttttacc aagcagggcg ttttcggaaa agcttctaaa gagttattgc
      841 tggcgtttag tgccccgatc gacttagaaa acatttcctc agaaactttg gcccaatact
      901 tagctgagtt aagcaggaac cgaatcaagg cagaaaaagc ggaccaattg aagcaagcag
      961 cttctaactc ctttggagta aaatttgctc aagaagcgtt caccttccaa ctacgttcaa
     1021 tgattgaaca attaaaattc attgaaagcc aaataaagga aacggaaaga gaaatcaagc
     1081 acataatgga cactttaaat tctgttattc tcacaattcc tggaattggt cctataaacg
     1141 gtgcgactat tcttggtgag atcggagata ttcaaaaatt ttctaacccg aaaaaactcg
     1201 ttgcttacgc tgggatagac gcttccgtga cacaatcagg gcaatatgag gcaacgcata
     1261 atgttatgag taaacgaggg tctccatatt tgcgaaaagc cttgttttcg gcggctttag
     1321 tagcttctca atgtgatcct gtttttagtg cgttttacca gaaaaaagtt tccgaaggaa
     1381 aacatcatct aactgcctta ggtgcggttt ctcgtaagct ttgttacgtg attcatgcta
     1441 ttttgactaa aaacgagcct tatataattg tttctgatta gctattgaac attcaaaaaa
     1501 actaatggta agaagcttat ttgttgtacc cttttttcgg aattatctgt tataaaatat
     1561 tttttgattt ggctaatttt tacttgactc tatatagtta gtctctctga taaaatttta
     1621 atttgtagcg aaacaaatta attacaagga gatcggatat gtccataaaa aaaacacgtc
     1681 tagtcctatt attggctagt ctcattcttt gccttgcttc ttttctacca gctgttgctg
     1741 tgcaagctga agatacgttc aaagtaaatg caaaagcagc ttttgctgta gatgcgcaaa
     1801 gcggtaagat tctttatgat caagatggag aaaaaaccat ggggatcgct tcaatcacaa
     1861 aaatcatcgg tctttacatc gtccttgatc aagtaaaaga aggaaaactg tcatgggatg
     1921 ataaagtttc gatttcagat tatgcagaaa atttgagtat caccccagat ttatcgaatg
     1981 ttcctttgca taaagaaaac acctacactg tcaaagaatt attcgactct gcaatcatcc
     2041 agtcagccaa tgcttcaatg gtggccttgg cagaaaaaat ctctggcagc gaagctaaat
     2101 tcactgaacg aatgaaagaa caattgaaag attggggaat caaagatgca acaatcgtta
     2161 atgcatcagg attgaacaac tcttatcttg gcgaaaaccg accagaaggc actggagaaa
     2221 acgacgaaaa tcaaatgtct gctcaagatg tggccatcgt tgcccgccat ttgatcttgg
     2281 atttcccaga aatactggat gtatcaagta ctacgaccca aatgtttggc gaaaatacgc
     2341 aatcaccagt ggaaatggtc aactggaact ggatgctccc aggattcgtt aattacaagg
     2401 aaggcgtgga cggattaaaa actggtacga ctgagcttgc aggtgcttgt ttcgttggta
     2461 cgatcactaa aaacgatcaa cggatcatca ctgtcgtatt gaatgcggac ggccatgcgg
     2521 aaaacccgag tgcccgcttc aatgaaacag accgtttgat ggactactgc tatgacaatt
     2581 ggtcagaaaa agagctcgga aaagctaacg caagtattcc tagtctaaaa accattgatg
     2641 taaaagacgg aaaagaatca acagttcctg ttgtgcttaa aagccctgta aaagtatggg
     2701 tacgaaacga tatggatact ggcaaattaa cgatcacgcc aacacttgac aagaagcaag
     2761 tcactgacaa tgagatccaa gcacctatgg ataaaggtac aaaaatcggt accgctacga
     2821 tcaccttggc agacgataaa ttaggctatt tagaagacaa caatgcacca tcaacagaga
     2881 tcattacgga taaaacagtg gaaaaagcga atatattcgt gattggctgg cgacgcgtcg
     2941 cagaattttt cgacaacttg ttttaattga acaataagaa aaacatcttt ccatcggaaa
     3001 gatgtttttt tgctttttct agtttaaata acagcttttt tcatctattt tcgttatact
     3061 gtataagaaa aaggagttga atttatgaat ctaaccagag atctgatctt tttagttctc
     3121 tatcttattg gggtattctc tatcgtttat tggcgaaaac agaagaagga tccttccttg
     3181 ttcttttacg gcagtacgtg gacatttacg gtaatcttaa cttattttgt cgtgttggaa
     3241 attttttatt cgacctttta ttttttgatt ccattgttgt tctttagtat ctttgctttt
     3301 tcttatttca cagaaaaacg aaggctgctg aatggtcttt tattcaatgt tttcctcatc
     3361 agtttcggca tctacttgtt tgtcttgctg tatgaaacgc aaaatatttt ccttggcgga
     3421 ctgatcgcct tgatcacgat tccattgcta cttgtccttt tatttggtat ttatggattg
     3481 attgtttttc ttttttggaa cggggtcaca gttttacgta gagaatcgca ttcattagct
     3541 aatcttttaa cgttgatact agccattttt cttacactgt ttcttgtttt tgactttttc
     3601 ttgttaaaat atttgccgca atggataaat gctttgtttt tctgcgttcc cctgatcttg
     3661 atctatctat ttatcgtatt ctacaacttt ttgactgtct cattcttgta tcagttcaac
     3721 cggcctcgat acaatcaaga cttcatcgtt gtgttaggcg ctgggttgat caatggagaa
     3781 acagtttctc ctcttctagc caagcgaatc aataaggcaa ttgcttttta tcgggctcaa
     3841 agccgtgcta cattaaatcc gcccatctta ttgatgtctg gcgggcaagg agcagacgaa
     3901 aaagtaccag aggccattgc gatgaaacaa tatgccatgg aacaaggaat ccctgaaaga
     3961 gacattcttg tcgaaaccaa ttcgacaacc actttggaga acatgctcta ttcaaaggaa
     4021 atcatggatc agcaaatgaa agggccatac cgcgcgatct ttagttccaa caattaccac
     4081 atttttagag ccggtcttta tgcgtggcaa gccaaattga aagcaaatgg tattggtgca
     4141 aaaacagctt tctactactt gcctaatgcg tttttaagag aatacatcgc catcttgatg
     4201 atgaacaaaa aaagacatat gatcatttgc ggattgcttt tcgcaggctc cgcattctta
     4261 tcgctgatta ctttactttt ctaaaaaaac aaggtgtgac aaaacatatg tcacaccttg
     4321 tttttttctt tcattctctt aactattggc aaaaagagta tccctttttt gaccgctact
     4381 tgctgggcag aataaatacc gctgttgcct gaacacataa agctgatcaa gcagatcata
     4441 aagaagtata ctgccgcttg tgaaccgaac aattcgatcc ccatcacaaa gcaggcaatc
     4501 ggtgtatttg tcgcaccaga aaatactccg ataaagccca atcctgcaag aaaaggaaca
     4561 gaaacatgaa gcaaaggcgc tagtgctgca ccaagcgttg caccaatttc aaacaatggt
     4621 gtcacttctc ccccttgata tcccgcgcct aaagacaaaa ctgtgaagaa taacttccca
     4681 ataaaatcat agacatgtgc ctgtccatta aatgattctt gaagtaacgg aagactcaat
     4741 ccgagataac gttgtgtttg caaaatcaat gctgctgcca caactacagc tccacctatc
     4801 gtatttcgcc aaacaggatg aggaatccat tgtgcatatc gcgcttttaa aaagacgatc
     4861 gaacggctga atacccaacc aatcaaacca aaagcaatcc cagcaacaaa aactttgaag
     4921 aacagcaaca tactccattc aggaatcaca cccatttggt aatgtagatg agaaacacca
     4981 tagctttctg tcatgaaatt tgcaaacagt gcagcaaaga agctagggaa gatcgcttct
     5041 gttcggactt tcccgattgc caacacttct aatccaaata atgttccagc taaaggtgtg
     5101 ccaaatacag aactaaaacc cgcactgatt ccactgataa tcaagatttc ccgttctgct
     5161 ttatccaatc ggaacaaacg tgctgtatta tctgctaacg atccccccat ttggactgct
     5221 gttccttcac ggccaacaga ccccccaaat aaatgggtag ttatggttcc aaacagtgta
     5281 agtgtaataa gccgcaacgg gattttttct tctccaccat ttccttgatc gatcactaag
     5341 ttgtttccac gactagaaag accgccgtga tacgcataga gataagcaaa aagcgcacca
     5401 ctaactggta gtagataaag gagccaagga tgtgatagtc ggatatctgt cacaactgtc
     5461 aggctattta agaaaaaagc agacaagctc cccatgaata taccgagaat cgctgtgatc
     5521 accagccact tcagcatgta cactaaaatg cgcattggac tcggtaattg tttttccata
     5581 aaaaattcct cctcaataaa aaaacgccta ctaaagaaag gggctgtccc cagactttag
     5641 taggcgtcat tagctatctt tcgttagcat ttaaaggcga acaccatcac ctattcactt
     5701 ccattaaaag atagcatatt tttctgtaaa aaacaataaa attatcgttc tttctttaca
     5761 cgttcagcaa attcatagaa aggagccaat ttcgttaact gttgattaca ttggtcacta
     5821 tcatgacaaa tataattccc ctttttcgta tacgtgccat cagaacctgc tttcgttgta
     5881 gaaagaaaaa gcgacacatt cgatgttttc tgacagattg cacagattcc cttatttatc
     5941 gttggtgata aagtaccgct aatccctttt aatttttcat cttcgtaata gaccatgaat
     6001 tttttctgcg tacctggatc attccagccg atgtacgtat gttcttgcaa gtcgatttca
     6061 ttccaagctg gttgtttgag tttttttact tttcggaata atttctcgat ttgtggtttg
     6121 ctaggctgca taaacggctg aacgatttgc ttcagcttct ccaatgctct ttctgcttgc
     6181 gcttttgtca gttttacgtc taaaactgtc gttaaaaatg tatcgatttc tggcagttcg
     6241 tgatttaata tgtctcgaat cttttcagct gctagtgcct gaactgtttg taccgttttt
     6301 ggatcattga cagatgagta cgcatttaat aactgttcta cttctctttt tataaaaaaa
     6361 tattggtatg gttttattgt ttgttccatg attttaaagt cctttcttct aaccaaagtg
     6421 catactttgt agaaaaaagt gatcttctgc aaagtatgtg ttacctgaag atcccggact
     6481 cttttaaata agatacgttt tgcatcttat catcaccccc tttaatgctt attcttctat
     6541 gtacatgatt cattcgataa ataaaagtaa accgtatctt gaacagatag tcaactgcaa
     6601 acagaaaaaa ggactgtgac aaaagtcgtg tcaaagtccc ttgtttcgaa tgaatggtgt
     6661 tcaaaagctt gcatctgcgg taataagccg aaacttcaca aaaatttgag aagcaatttt
     6721 cgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctca atatggtcta
     6781 ttattggttt tacctgttaa atcgaggatt ctttcccaaa gtaacgttgc catctcttac
     6841 gcatacttgc agacatgggc tgagccgctt ctttgactgc gcagtatttg tccacgaatg
     6901 tcacaatata tccttcttta tacttaggcg gtgcgatcgt tgcaccccac atgtgcttca
     6961 aaatgatatc tcgctcaaga tccgacaaat cggtgatttt ttctgcattc tttacagcaa
     7021 ttcttggatg gatatacgca tgtgtcccct cgtcaaattt cgtcgtacgc caatcatagt
     7081 agaacagatc atgcaaaagt ccagcacgcg ctgttgcttt agcattgcca ccccattttt
     7141 ttgcgagcag ataactatag taagaaacac tgatggaatg ctccaaacga gtggaatgaa
     7201 catgctgtgt gtaattcgct aattttttca ctgcttctgt ttctaaaaga tcttgtacat
     7261 aagacatata ttcttcgtct tcacgccatt tttctgtcat aattatcgat ctgccacctt
     7321 tttgaatttc attttcttct gaaattgatc taagtatacc accagtgttc ggattattct
     7381 agtcgctcta gataaaatag ctaaagctta atagttcctt aatcataaag aaaaatgata
     7441 aagaacgatc cctgctgcga ctccgacatt caatgattcc gcatttcctt tcattgggat
     7501 atatacgttc tgatcagccg aagacaagat ttcctgactt actccctgac cttcgtttcc
     7561 catgattata gcgaaatcct ctgttttttc tactttgtct aatgcatgtg cttcttcatt
     7621 taattctgta ccgtacaccg taaacccttt gcctttcatt acagggatca cttctgccaa
     7681 atctttttgt aacacaggca aatgataatt gcttccctgc atgcttcgca gcacctttgt
     7741 actataaata tctgccgttc ctttacctaa taaaacacca gccataccaa acgcatctgc
     7801 cgtacggatc atcgttccca catttccagg atcctgtaca ttatctagta aaagccagcc
     7861 tcctgaaaaa tctggcgtat cttcagatgt taacgtcatc actgctaaga tcccttgcgg
     7921 tgtaggtaga tctgacatag cggtcatgac ctcatctgaa actagaaaag tagaaatctc
     7981 tttgtttaca gccagccagt ctccccattc tttcatacct ctctgtgtca caaaaagttc
     8041 ttttatttct gcatcagacg acgcagcttc ttcgatcaga tggaaccctt ccaaaagata
     8101 ttctgccttt tcttctctgt atttcttttt atgtagtttt tttaattctt tgattcttgg
     8161 attttttact gattggatct ctttcacatt cctcacctca actgattata tcatggaata
     8221 ggtgcttgag cttgtttcat cctaaaaaaa tcggtatcat tgatttataa aacaggaaag
     8281 aaaggtgacg aacatgcgca aagttaaaat gaatgtccaa ggacgtgtcc agggcgtggg
     8341 atttcgctat atgaccaaga tggttgctga tcagttaggc gtgaccggaa cggtaaaaaa
     8401 cgaagacgac ggctcagtca caatcgaagc aatcggaaat gacgacatca tccaaaaatt
     8461 tatcgaagaa gtcaagaagt ctccgagccc aagtggacga gtgcagtatg tggatgtcca
     8521 agaagatccg atgattgagg aacgaaagaa atttgatgtg gtgggataag gttgtttttg
     8581 aagcgctttg cctcgagtat taaggagaaa cattttccaa atagcttctc atattttgaa
     8641 atgtaatgac ttaataccga gaggcagctg ataaaacacc gtcaataagg ttgtgaaaaa
     8701 gctggtctgc atcaagcatt aaggactggt tgtgaaaaat agctctccat atttttcaac
     8761 aggctggact taatgccgta gatgcaagct tttgaacacc gtcgataagg ttgcgaaaaa
     8821 gctgatctgc atcaagcatt aaggactagt tgtgaaaaag tcgcttagtt ttgagtatta
     8881 aggaaaattt tgtgaaaatg gctttccaca tttttgcata aatttgactt aataccaaga
     8941 aactgacttt tgaacatcgt cgataaggtt gtttttgagg cactttgcct caagtattaa
     9001 agacaggtag taaaaaagct gatctataac ttgcaataaa tttgcttaac ccatattttc
     9061 cagacagaac aaaaaagttt ttagtttttt aaataagaaa gaaacacttg gaatcactct
     9121 tttctttcta agtatttctt tcttgatttt ttgtcttttt tcaaaatttt tcgctacgaa
     9181 aaagaacgaa tatttatatg aaatttctat gaagttgatt gaattccatg cgctttttta
     9241 gtatagttaa gtttgtgtaa aaattataga agaggaagta ttctatgaat aaatggaaaa
     9301 actggttatt gggatccgga cttgtcagta tcctgctctt tttatctggc tgtgtgcgga
     9361 tggattcaaa cggaaaccct gacacttcag gaatcgttta tcgagtactc gtccatccta
     9421 tgggccaagc gattacatat ctagttgaga atttcaattg gtcttacggc tgggcagtta
     9481 ttgcaatgac tgtgatcgtt cgtatcatca tcttgccact aggtatcagt caatcgaaaa
     9541 aaacaatgat ccaatctgaa aaaatgcaag cgttaaaacc acaagttgaa gcagcacaac
     9601 aaaagctaaa agctgcaaca acacgtgaag aacaaatggc agcacaagca gaaatgcagc
     9661 aagtatatcg cgaaaatgga ttgagtatga ccggcggaat cggctgtctg cctctattga
     9721 tccaaatgcc gatcttctct gccttgtatt ttacggctcg ctacacagaa ggtatccgtg
     9781 aatcatcttt ttacggcatc gatctaggct caccaagtat ggtgcttgtg gccatcgcag
     9841 gggtcgctta tctgcttcaa ggatatattt caacaatcgg tattcctgaa gaacagaaaa
     9901 agacgatgcg tacgatgctg atcgtctctc ctgctatgat cgtcttcatg tcgatcagtg
     9961 cgccagccgg tgttacactt tactgggtag tcggtggtgt gttcagctgt ctgcaaacat
    10021 tcatcacaaa cgttataatg aaaccgcgtt tgaaagcaca agtggcagaa gagttgaaac
    10081 aaaacccgcc aaaacaagtc gttactccaa gaaaagacgt gacagaatca gcaaagccag
    10141 ctgacaacaa acaagcaaca aaacaaatca aacaaccaaa aaacagtaca aacaacaaag
    10201 gccgcaacgc aggaaagcag cagaagcgga aataaaaata tatgacaaaa aactgtagac
    10261 aaataaaaaa atttctttgt tctacagttt ttttgt
//
LOCUS       DACEQH010000079         9965 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.081, whole genome shotgun sequence.
ACCESSION   DACEQH010000079 DACEQH010000000
VERSION     DACEQH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.081"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            8..81
                     /locus_tag="IW833_002391"
     tRNA            8..81
                     /locus_tag="IW833_002391"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="IW833_002392"
     tRNA            101..173
                     /locus_tag="IW833_002392"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="IW833_002393"
     tRNA            201..274
                     /locus_tag="IW833_002393"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="IW833_002394"
     tRNA            288..361
                     /locus_tag="IW833_002394"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="IW833_002395"
     tRNA            385..474
                     /locus_tag="IW833_002395"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="IW833_002396"
     tRNA            487..560
                     /locus_tag="IW833_002396"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="IW833_002397"
     tRNA            579..654
                     /locus_tag="IW833_002397"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="IW833_002398"
     tRNA            656..726
                     /locus_tag="IW833_002398"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="IW833_002399"
     tRNA            740..813
                     /locus_tag="IW833_002399"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="IW833_002400"
     tRNA            838..925
                     /locus_tag="IW833_002400"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="IW833_002401"
     tRNA            947..1018
                     /locus_tag="IW833_002401"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="IW833_002402"
     tRNA            1041..1130
                     /locus_tag="IW833_002402"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="IW833_002403"
     tRNA            1143..1216
                     /locus_tag="IW833_002403"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="IW833_002404"
     tRNA            1235..1307
                     /locus_tag="IW833_002404"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="IW833_002405"
     tRNA            1311..1383
                     /locus_tag="IW833_002405"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="IW833_002406"
     tRNA            1388..1458
                     /locus_tag="IW833_002406"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="IW833_002407"
     tRNA            1471..1544
                     /locus_tag="IW833_002407"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="IW833_002408"
     tRNA            1556..1627
                     /locus_tag="IW833_002408"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     gene            1893..3086
                     /locus_tag="IW833_002409"
     CDS             1893..3086
                     /locus_tag="IW833_002409"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ1308478.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQIAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="IW833_002410"
     CDS             3216..4688
                     /locus_tag="IW833_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1308479.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="IW833_002411"
     CDS             4771..5559
                     /locus_tag="IW833_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ1308480.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="IW833_002412"
     CDS             complement(5626..6294)
                     /locus_tag="IW833_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1308481.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="IW833_002413"
     CDS             6409..8121
                     /locus_tag="IW833_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1308482.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9896
                     /locus_tag="IW833_002414"
     CDS             8124..9896
                     /locus_tag="IW833_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1308483.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2901 a         1780 c         2279 g         3005 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agatagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     9901 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     9961 ggcag
//
LOCUS       DACEQH010000080         9542 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.082, whole genome shotgun sequence.
ACCESSION   DACEQH010000080 DACEQH010000000
VERSION     DACEQH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.082"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..813
                     /locus_tag="IW833_002415"
     CDS             <1..813
                     /locus_tag="IW833_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAQ1308484.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="IW833_002416"
     CDS             807..1229
                     /locus_tag="IW833_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308485.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            1531..1911
                     /locus_tag="IW833_002417"
     CDS             1531..1911
                     /locus_tag="IW833_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ1308486.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            1893..2807
                     /locus_tag="IW833_002418"
     CDS             1893..2807
                     /locus_tag="IW833_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1308487.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            3047..3736
                     /locus_tag="IW833_002419"
                     /pseudo
     CDS             3047..3736
                     /locus_tag="IW833_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3856..3942)
                     /locus_tag="IW833_002420"
     CDS             complement(3856..3942)
                     /locus_tag="IW833_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1308488.1"
                     /translation="MFELFICPLLVGVCISVFSYWLNNRNKK"
     gene            4588..5520
                     /locus_tag="IW833_002421"
     CDS             4588..5520
                     /locus_tag="IW833_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1308489.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEEAEKQSISNELKQDLLENLQNLFD"
     gene            5537..6007
                     /locus_tag="IW833_002422"
     CDS             5537..6007
                     /locus_tag="IW833_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ1308490.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTL
                     VVSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLD
                     QQQQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
     gene            6091..6408
                     /locus_tag="IW833_002423"
     CDS             6091..6408
                     /locus_tag="IW833_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1308491.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDT
                     ENLIQFAKLFDLSLDNILLGEEKIMVKNYGKKSYKNTNTIISFCNFFKFSFHLNSVRI
                     LLF"
     gene            complement(7003..8151)
                     /gene="tnpB"
                     /locus_tag="IW833_002424"
     CDS             complement(7003..8151)
                     /gene="tnpB"
                     /locus_tag="IW833_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1308492.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(8168..8572)
                     /locus_tag="IW833_002425"
     CDS             complement(8168..8572)
                     /locus_tag="IW833_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ1308493.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            9201..>9542
                     /locus_tag="IW833_002426"
     CDS             9201..>9542
                     /locus_tag="IW833_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAQ1308494.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         3460 a         1603 c         1580 g         2899 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acatgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagctttt
     1321 tttgtattgt ggagcgtggc ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcactaaa tacgtaggta tttggctagc aaatatagag cttgccaaac
     1441 ccttttagtg agctttactc attgtacctt tttaggtaca atggtattgc gaacgatacg
     1501 tccgtaggac gtactgaaag ggtgggactt atgagcgaac aaaatcaaaa tttggctagc
     1561 aattcttcta aaaaatatac ctatcgatcc gagccaaaac aaatcagttt tcgagtgagc
     1621 gaatccgaat ttgcaaagct gaagcaatcg gctgaagctt tgcaaatgag tgtgccggct
     1681 ttcgtgaaag ccaaggcaca aaacgcgcgt ctggtgacac cgaaagtggc gccagacatt
     1741 gcccaggcaa tcgcgcgcga tttagccaaa gctggaggga atatcaatca aattgctaag
     1801 tggtgcaata cacaccaaca tgacgttgct tctggtgacg ctcaacgttt atccgaaaac
     1861 ctaaaaatca tgcaaaagga gctacaaaag atatggcaac aattaaggta agccgaacca
     1921 cttcttgtag tcgtgcaatt aattacgcag aaccacgagc aaccattaaa acaggaataa
     1981 actgtgacgt cgattatgcc aaaagtgaaa tgaaacaaat tcgcatgctg tatggaaaag
     2041 acgaccgtgt acaggcacac ttattgattc aatcttttcg accaggagaa attaccgctg
     2101 aaaaagctaa tcaactaggg gcagagtatg caaaaaaaat agcccccgat caccaaacag
     2161 ctatttacac acatacagac aaagaccata ttcataatca cattgtcatt aactccgtta
     2221 atctggagac tggaaaaaaa tttcaagctc atggtcaagc atttctaaac aaatgttatg
     2281 acctaaatga cgagatttgt cttgcacata acttaccaat cactgagcga ggaaagaaac
     2341 cagaaaaacg aaccatgtct gaaataaaat taaaagaaaa aaatgaacct gtttggaaag
     2401 acgaaattcg gattgcgatc gatcaaacaa tgaaaaacaa aaaaaccaga acctatgacc
     2461 aattttgtaa ttccctcaaa acattgggga ttcattgctt caatcgcggg aaaaatttca
     2521 cttacgaatt gatccacaaa aagaaaaaag tacgatccaa cagactagga aaggactacg
     2581 aaaaggagac gattttacat gagctggaca gacgagaacg aacctataac caacagcgaa
     2641 ccaacgagct acgaagagct cgagctagca ttcgcgaaac tgcagaagaa gtacgaagag
     2701 aacaacgaac tcttacgcct gaacaaccgg aaattagacc ggctcgaaga aaccatgaac
     2761 aacacgttga aagaggaaca gaccaccaac aagaattatc gctctaaaac gttaaaagac
     2821 atagaaaacg aaataaagaa acaacgtgct tttctcgttc cttatacgca agaaaggata
     2881 caaactacga ttgatttaca agttaaaaaa ctccatcaag agctattaga ccagcgaaat
     2941 aaggaacaga aaaaggtgct tattcaacga tcattggatt ttcttgcccc cttatgttgc
     3001 ttagtcctga cactgttcgc attttggtta gtatggggag gctaaaatgg cattttattt
     3061 accctatcta ctcatttttg ttagtatttc aggaagtatt tggctcatct ataaaatttt
     3121 tcaaacaaga tattcattaa aaggctctaa aattcgattt aagcgatttt tctttcttgg
     3181 ctgtatattt ttccttgata atcgtttcaa gcggcttatt cggcgtttta gagggaaata
     3241 agcgcgtttc tagaagtatt ttattaggaa atgtcactca aaagtatgaa tcagcgcgtg
     3301 ataaaaagaa aaaagagcaa gcattagcac aaaaaatgga agagtttacc acttgttatg
     3361 aggaaatgaa cgatattttg tgaatcaaga aaaacgtttg acggataaaa acatggaaaa
     3421 actcactcgt ctttatcaaa acttgccaga aaaacagcaa aaagaggttc aagacaacta
     3481 tgagcgaacc aaaaaagacg tgcaatatgt gaaagatacg aaagtggaag aagcttgtag
     3541 tgatttattt ggagatacaa ttcctttttc aactagcgaa caagaacgaa aagaacgaca
     3601 acaatcagtc acatatgaac gctatgaaat cttgcttcaa caagcaatta acatacaaaa
     3661 tctgactaaa aaagaaacag ctttaaatta tttaaaaagc gtcaaggaat ggttagatca
     3721 gcaacagcaa aattgaattt gattgaaatt catgttaaac taaacatgca aatgttcaaa
     3781 cattagatac acgaaagccc cagagaggtc ggattctctg gggctttttt taggttgatg
     3841 tcgcctttac tgattttact tcttgtttcg gttattcaac caataactga atacagagat
     3901 acaaactcct acaagaaggg ggcaaataaa cagttcaaac atcagataca cctccttcca
     3961 gtcgcaagac tgtcggatag gcgacaagtt attataccct acaaaattaa atctgtgtgg
     4021 caattttaag tatctcttat ttccgggttg ttagggcttg ccctgaccgc ctgtagggcg
     4081 ctttaaattc cagataaagt tttttatact aagactgtct taaaacggct tagacaagct
     4141 cttagagcct caaccctcct ttttgaacaa tggttttagc aaaggagctg gaggcgactg
     4201 cgcaggtata atgtgagagc gtagcgatca cacctttttg gcgaccgtag ggagcgaggt
     4261 tttgaattag cagtttagaa aagttcgcga acctgtttaa agaaaaaata attgtttttt
     4321 taggtgggaa attttgtctc cctcttttat aatctttttt taaacctctt tttaaacctc
     4381 tttaacgtcg gctgtgtcct tactccccca aggtatacaa aattttatag gcatgaaatt
     4441 atctgtttat aggcatgaaa ttatctgttt ataggcatga aattatctgt ttataggcat
     4501 gaaattatct gttttaggta ttgtttttag acataatatg tcctaaaatg atgtcaataa
     4561 aatcatttag actggagtaa caactaaatg gcaaatgaaa tcgtaaagta tgaaccagaa
     4621 ttaaatacca ttcctttaag aaaatttacc ccaatagaaa tgaacttgtt tttttctatt
     4681 gtctctagaa tgcgcgataa aggtaatcaa aaaatacgct tttatttcag ccaacttaaa
     4741 gatttaagtg cgtataagca gactgcagga gaagtcttta taaaagattt ggcaagaaca
     4801 tacgatcatt tgatggattt acgtttcgga agaaaaagca atggtggatt aacaatagaa
     4861 cgatttacaa tgtttactga ttttgaaatt catggagaag tcgataaccc ttatgtagat
     4921 ataaaagtcc acgaaaaagc tttgccttta cttaataatc tagaaagctg ggtacgtttc
     4981 tctttaacag aatttcgaga tttaaatagc agttatgcta aaacaatgtt tagattgcta
     5041 aaacaatacc gaactcaagg atgggctgaa ttttctaagg aagatttttt agagctatta
     5101 gatattccta aaagctatag acaaacaaat ataaatcagt ttgtactcaa accaataaaa
     5161 gaagaactaa ctccactatt taaaggatta actattcgta aaaaatatgg taaaggtcga
     5221 ggtaaacctg ttattggtta tcgttttact tggaaagctg aaatcaacca tgctgacgat
     5281 ttttcaaaag ggaagcaaga agatttacgt ataaaattat ttaatattga acacaatgga
     5341 gaattaacac aaaaagaaaa atggcgtgct aaagatcgaa tactaaatct tccgttaggt
     5401 actcatgaag ctgattttaa aaaacaacaa caaaccgaaa aagaagaagc ggaaaaacaa
     5461 tcaatctcaa atgagcttaa acaagattta ttagaaaact tacaaaactt attcgattga
     5521 agcataagga gtatttatga tagataaaac gatcaaagaa ctcgcggaag aatttcatgt
     5581 ttcaaaacaa gcaattagga aaaggctatc agctgatttt agagaaaact atgtgcaaac
     5641 tgttactagg aacggagtga aaaccttagt tgtcagctac aaaggatatt tgcttttaaa
     5701 acaacatttt ttaggtagta acaccactgg taaccaagta gaaaccgttg ctagtaacga
     5761 tgaaaactcg attattgttt ttttacatga acagctccaa gaaaaagata ctcaaataaa
     5821 acaaatgcaa aagttactcg accagcaaca acaactcacc ttgcaggcaa atcaacagat
     5881 cgaaaaactg caagaacagt tacaattagc ttatacggaa gaacctgaag aagaggaatt
     5941 aacttctgtc aataaagaaa acaacaatgc aataacaaca gaaaaaaagt ggtggaaatt
     6001 ttggtaacaa ttaccaactt tccataaaga agaaatcaat ttctatagac tctatctttc
     6061 taagaataaa aaatatgttt aggtgatcaa atgactataa gtaaagtaat caaacaaaaa
     6121 agaatagaaa aacaactaac tcaagaagat ttagctgaaa tgcttttagt atctaaaaaa
     6181 accatttcta attgggaaaa tggtcgaaca ataccagata cagaaaattt aattcagttt
     6241 gctaagttat ttgatttatc actagacaat attttgttag gagaagaaaa aattatggta
     6301 aaaaattatg gtaaaaaatc atacaaaaac actaatacta ttatttcatt ttgtaatttt
     6361 tttaagttta gttttcacct taattcagtc cggattcttt tattttagcc attcaattgc
     6421 cacttggtta ttaatatttt ttctaagttt actattaata attaatacat taataattaa
     6481 tactttaatt attatcttta ataaacatag aaacttattt cttcacagca tatttccaat
     6541 tgttttatta atttttctat actttatata aaaagataga actatttagt aagataaaaa
     6601 agtagctaat tgtaattttc gtctaaaact caaaactagt aaaaataact ttattgtgtt
     6661 atttttattt ataaattatg aaaaaggacc tttcgaaatg aaaaataata gaatctacaa
     6721 aaatcagttg aattatgggc ttttattgcc agtttttctg ctatgtatca ttggattact
     6781 ttctttatat gttgctttat atcacgactc ttacactaat tcaaaaatca caaaagaatt
     6841 aagtgaacta ctcaccactt agctaaacct gacggttctt aacgcttgaa gtgggagctt
     6901 cttgggaata gagcatactt gatagcgtat ttctttacta acagcggtgt ctcttattta
     6961 ccaagctatc cccgtagttc ctacggttct tgatgattgg atctaagcca atcccataga
     7021 atgtagtctt agaccttcgg tcagaatatt gatactagca ttgatatcac gatcatgatg
     7081 agtatgacaa acaggacaag tccattctcg aatttcaaga gatttcttgc catctttatg
     7141 tccgcactct gaacaaatct gactagacgg aaaccattta tctactttga tgatttttcg
     7201 tccataccag tcagccttgt attgtaattt agtcacaaac tttgaccaag aaacatcaga
     7261 aatactttta gctaatttat gatttcgcaa catacctttt gtgtttaagt cttcaataca
     7321 gataatatcg tgatttttga tcatttctgt actcaactta ttcagaaaat cagtacgttg
     7381 attcattact ttttcatgca atctagctac tttgcgtttt tgtttttgat aatttttagc
     7441 ttcaaataga ttggtacctt tatttttagc caataaagca cgcctagaca atttgcgttg
     7501 ttcacgcttt agtttctttt ccattttgga cgtgaattta ttattatcaa ttttttgtcc
     7561 atcagaaaga atcgcaaagt ccataatacc taagtcaata ccaattgtgg aattagtttt
     7621 aggtaattcg ataatttctt ctttacacaa caaagaaata tagtatttac cgctagaatg
     7681 acgtgatatt gtagcagatt tgataatccc ttttggttgt ctatgaagct taattcttac
     7741 taatgacttc aatttaggaa ctttgatgaa tttactatca atcaaagcaa ctgtaccatt
     7801 ttgattattc gttgtataac tctgaacagg atttttctta cttttgaaac gtggaaatcc
     7861 aacggattta tcccgaaaaa aattcttgta tgctttatct aaattaagtt gggcattggc
     7921 taaagcaagg ctatcaattt ctttcaaaaa cggaaattct ttcttgtatt tagctggtgt
     7981 cggaaaagtc atttttttag aagaatcatt tttaacttct tcatacgcct tttttcggtc
     8041 atcaagcatt agattgtaga ccttacggac acaaccaaaa gatttggcaa agaagatttc
     8101 ttgttcctct gttggataga ttctgaattt gtatgctttt agttgttcca taaagttcac
     8161 ctactttcta tttatgattg tttccttgat tttggatata cttcttaatc acatcaattg
     8221 gtgcgccacc agttgtcaga agacaaaaac ttttagacca gaacatttct ttccaaagaa
     8281 attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt ttataggcat
     8341 tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga acgtggtctt
     8401 tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt tcaaaagtag
     8461 ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc gttactaaaa
     8521 caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc atgttatatc
     8581 aactcatttc ttgtatagac tgattgtaac atggaacaaa ataaaaaggc aatacgcctt
     8641 agttgtcggt cttcgagtta ccaacggtaa ccctcatacc gaacggcatt catcacccac
     8701 ctatagagga tgggcgactt ctgcctaatt ctgttaaatt caaattatta caacagtcct
     8761 aacgaagaat tgtttgtaaa acaaacggat aaataataaa aaagactgct ctttcgagca
     8821 gccagttaaa aaataatgag tgtttattct tgaaaaagca acgcgcttat gaagcgctac
     8881 caaaaattta tcatatttta tgaaaaataa caccccagta aaaaatgact ggtaaaaatt
     8941 cacaaagaaa cggaaagaag caactgcaga aaatcaagcc ttaaaaagag cttttgacct
     9001 agaccaatga aaaatcacaa agtgagcccc tgtatccctt ggtagagagg ctacttggca
     9061 tagtcgacat tatgttaact aacgctataa ataacgatta tgttcccaaa acaatatttt
     9121 atgaactata acaaaaattt gttatcgcat attgtatatt ttaattatag gtgtatacca
     9181 cttaaattta acacaaaggc gtgattattt tgaaacccaa aaagaaaatt tatctaggtc
     9241 tcctcttttc cttgatgatt ggaattttta gtataggaat tacaaatacc tatgcttcag
     9301 aaaattctaa tttcaatatc aactcccaac caattgaaga acaagcaatt aattttgcga
     9361 aaactgattt tgaacactcc ataacttcaa ttcttttaat ggaaaataac accgaaactg
     9421 atattaacta tactcttggt tcgccattta aaatgctcga tggagaaaat acggcaaata
     9481 gttctatttt ctttccagta ttttcagacg ctgataatac gattaaatat atttataccg
     9541 tc
//
LOCUS       DACEQH010000081         9464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.083, whole genome shotgun sequence.
ACCESSION   DACEQH010000081 DACEQH010000000
VERSION     DACEQH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.083"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            7..849
                     /locus_tag="IW833_002427"
     CDS             7..849
                     /locus_tag="IW833_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1308495.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            846..1796
                     /locus_tag="IW833_002428"
     CDS             846..1796
                     /locus_tag="IW833_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1308496.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1811..2044
                     /locus_tag="IW833_002429"
     CDS             1811..2044
                     /locus_tag="IW833_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ1308497.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            2375..2959
                     /locus_tag="IW833_002430"
     CDS             2375..2959
                     /locus_tag="IW833_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1308498.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            3090..3758
                     /locus_tag="IW833_002431"
     CDS             3090..3758
                     /locus_tag="IW833_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1308499.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            3760..5184
                     /locus_tag="IW833_002432"
     CDS             3760..5184
                     /locus_tag="IW833_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1308500.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            5202..5771
                     /locus_tag="IW833_002433"
     CDS             5202..5771
                     /locus_tag="IW833_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ1308501.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            5881..7635
                     /locus_tag="IW833_002434"
     CDS             5881..7635
                     /locus_tag="IW833_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1308502.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            7589..9361
                     /locus_tag="IW833_002435"
     CDS             7589..9361
                     /locus_tag="IW833_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1308503.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
BASE COUNT         3286 a         1245 c         1732 g         3201 t
ORIGIN      
        1 aataatatgc ttcaaaattt tattaatggt ctgtatgact ttcacttttt acagaatgct
       61 ctaattacat ctgtagttat agggattgtt tctggaacag taggttgttt tattatttta
      121 agaggaatgt ctcttatggg tgatgctatt tctcatgctg ttttgccagg agtagcaatt
      181 tcttatattt taggtattaa tttttttatt ggtgcgattg tatttggtct attagcttct
      241 atcattatta cttttataaa aagcaatagt gtaataaaag gagatacagc aattggtatt
      301 actttcagtt cttttttagc attaggagtt atccttatag gagttgcaaa tagctcaacg
      361 gatttatttc atatcttatt tggaaatatt ttagctgtac aagatattga taagtggatt
      421 acaattggcg tttctgtttt agtgttggtt acagttgttc tattttttaa agaattatta
      481 atcacttctt ttgatccatt aatggcaaaa gcaatgggga tgaatgtcag tttttatcat
      541 tatctattaa tgattctatt aactttagta gcagtaacag ctatgcaaag tgtgggaact
      601 atattaattg ttgcaatgtt aattacacca gctgctacag catatttata tgcaaacagc
      661 ttaaaaacta tgatatttat ttcggcgaca gtcggagcgt gctcatcatt gctaggtttg
      721 ttcattggtt atagttttaa catagcagca ggatcaagta ttgttttaac agcggcattc
      781 ttatttgtaa ttagttttct aatctcacca aaacaaaaat ttttaagaaa gaaggagaga
      841 aagttatgat gagaaaatgg aaagcagtac tgggaagtct ggggattttg attgctcttt
      901 ttatattcgg ggcatgttca acaaatagta aagacaaaga tacagtggct tcgaacgaaa
      961 aattaaaggt agtagttact aattcgattt tagcagatat tactgaaaat atagcaaaag
     1021 ataaaattga tttacacagt atcgtaccta ttgggaaaga tccccacgaa tatgaacctt
     1081 tgcctgaaga tgttcaaaaa acttcaaaag cagatttgat tttttataac ggtgttaact
     1141 tggaaactgg aggaaatgct tggtttacaa aattagtaaa aaatgcgaac aaagaggaaa
     1201 acaaagacta ttttgcagca agtgatggca tagatgttat ttacttagag ggtcagagtg
     1261 agaaagggaa ggaagatccc catgcttggt taaatttaga aaacggtatt atttacgcta
     1321 aaaatattga aaaacagtta gcggaaaaag atcctgataa taaaaaattc tataaagaaa
     1381 atctagataa gtatattgaa aaattggatt ctctagacaa agaagctaaa tctaaatttg
     1441 cttcaattcc gaatgataaa aaaatgattg ttacaagtga aggatgcttt aaatatttct
     1501 cgaaagcgta taatgtgcct tctgcttaca tttgggaaat caacactgaa gaagaaggaa
     1561 caccagatca aataaaacac ttagttgaaa aattacgcac aacaaaagtt ccctccttat
     1621 tcgtagaaag cagtgtggac gatagaccaa tgaaaacagt atcaaaagat accaatattc
     1681 ctatctattc aacgattttt actgattcaa ttgcagaaaa aggacaagat ggtgatagtt
     1741 actatgcgat gatgaaatgg aacctggata aaattgctga aggcctttcg aaataatttg
     1801 gagggtaggt atggtacaaa ttaaaacgac tactatacta atttatttac tagcaattca
     1861 ggaattatca aaaagaaaaa agaaattaaa aaataatgat ttagcaaaag ttctaagtgt
     1921 tagtccagca tctgtaagtg agatgctggc taaacttaga tatgaggact atttagatat
     1981 aggatttcaa ttaactgata aagggaaaaa atttattgac gattacaaaa aaggttttaa
     2041 gtaaaagact ttatttttag ggaggataag aaaatggcta ttggagtgat tatgatagga
     2101 cttaccctaa tagctggata taattttttt aaatctttct cggattattt tagaaaaggt
     2161 gaatgattta gatgagtcta ttaattcttg taggattgct ttttgtatca gttattttgc
     2221 tctttctcat tagtttagct ctaaggaatg agttcagtaa tagacgttaa cgattgaaac
     2281 ttcaagtcct gtgtaagata atatagttaa ttcatcttat taggaggaag tacccataga
     2341 aaccagagat tatattacaa ctcaccaaaa attaatagaa agtggactaa gccattttga
     2401 aaaagaagga tttcagcaaa ctaagataag gcaactgtgc ttgaccgcag gcgtaactac
     2461 gggtgctttt tataaacatt ttacttctaa ggaagcgtta tttgaagaaa ttattcagcc
     2521 gcatattaat gcgcttatta atatgtataa tgaagagttt accaaatacc tcttaaactt
     2581 agaaaatata gatatagaga aaagttggag agagaacttc aatgaattag atccatttat
     2641 taattatatt tttgagaata aacgtatatt cgatttggtt ttattccaat cagctggaac
     2701 caaatatcaa gatattgttg aaaaaattac atcatttgtg acaaaaaaga cagtctgttg
     2761 tctagataag ttaaagacta tcggaatgat caatcaaaat ttagtcttta atgaagaaga
     2821 aatccacttc tacatttact cattctacgc tacattctat gatatattga agcatagcta
     2881 ttcaaaagaa aagactatgc tattaaccag aagattgtat caattttctg ttcctgggtg
     2941 gattgaatta ctgagttaat taaaaaaaag cttgcattga gaattgttct cagttatgct
     3001 ttttgataag atcgcactgt tatctgaata acggtgcatt tttgtcagct taagataaca
     3061 gtgcgatctt attttttgga ggtgaaagag tgtcaaaaat caatccgtta accttgatta
     3121 ctttaaatat tttttttccg gtcattattt ttttaggaaa gggaaatata tttcaagtgg
     3181 ggtgtttcat tattgctaca gttttagtat tgatgtttcg taaaataaag acatgtattt
     3241 atttcatctt gatttattca gttctgtttt tagtaatgaa aattattgat gctatcaaga
     3301 tagagttttt tactatattt tttgggacat ccttatatat tgtatttcga atgttgccgg
     3361 tcgtaatgat ctcatggttg ttggttagtg tctatactag caatgaatta ttatcctctt
     3421 tagaaaaaat acatttaccg aaaaaaataa tgttaagtat tacagtagtg atcagattct
     3481 ttccgactta tcgttctgaa ataagaatga ttaaagaatc tttaaaaatg agaaacattc
     3541 gcttgtcttt ttttcagcct ctgcaatatt tggaatttct gttagttccc gttcttatac
     3601 gtgcaactat gattgctgaa gaaatgaccg ctaatgcaat aacaaagggg atagaatctc
     3661 ctgtgaaaag aagttcttac tataaaacaa acatgagctt attagactat atcatcctgt
     3721 tttccgttac tatttttttc ttaagtattt tgatttgagg tgaaagaatt gtttgaattt
     3781 aacaatgttt ctttttcata taaggatgtt aaggaacaag cgcttgttga ccttaatttt
     3841 tcaataaaaa aaggcagtct tacattgatt actggttctt ctgggagtgg gaaatccaca
     3901 ctattaaagt taatgaatgg acttattcca agactttatg acggaaaatt ggttggggaa
     3961 attttatttg aaaataactc gctacaatcc ttaacacaag aaagtatatc aaaaaatatt
     4021 ggctacgtaa gtcaaaatcc tagaggacaa ttctttacaa caaatacaac gagtgagtta
     4081 gtattttcga tggaaaactt tggatttaca aaagagcata tggataaaag aatcaatgaa
     4141 ttagctgaat tattagatct ttcaaatata ttagataaaa acattttttc gctatcaagt
     4201 ggtgaacggc agaaaatttc tattggatgt agtttatcgc taaactctaa attaattata
     4261 ttggatgaac cgtcttcaaa tttggacttc aaagcgacta agaaacttgc tgacttactt
     4321 tgtgatttaa aaaataaagg atttactatt gttatagctg aacatcgttt ttactatttg
     4381 aaagaattaa ttgatcaagt tctatttgtt aaaaatagaa ctgtaaaaac tacaactatt
     4441 cagttgttaa aaactgaaaa caataacgca ttaaggaaat ttgacatttt taatagttcg
     4501 attgaagttc cctatatcga aaaggtaagt gcagatgtat taaaagttga agaaattact
     4561 tatgaaaata ttctaaaaga cataaatttt gatgtcaaag ttggagatgt tattggtttg
     4621 gtagggaaaa acggtgtggg gaaaacaacc ttactaagaa tactcatgaa gattattcgc
     4681 cctaccaaag gaaagataat cgaaaataaa aaaaatttgt catcgccgtt tttagtaatg
     4741 caagaaatgg attaccaatt tttcacagaa tcagtgagga gcgagttagc cctggggaat
     4801 gaaatagtta gtaaagatca gcaagaaaaa attttaaaaa aaatggaact ttataaaatg
     4861 aaagatcaat taccgtttga tttatccggt ggagaaaaac aaagattatt ggtttctata
     4921 gcttcattgt caaaaactaa tttatttcta tttgatgaac ctacaagtgg gttggattat
     4981 atcaatatgg aaaggattgc tgaccttatt aaagacttaa aagaaaaggg tgcagtagtt
     5041 atagccactc atgatccgga atttctttat aaaacatgca atagaattat ttatttaaaa
     5101 aatggtggaa taaaaaaaga tataaaactt agagtagaag attcaacttt gatagaacag
     5161 atttttaatg acataacgaa atagaattga ggagaatata tatgaaaaca aaagattata
     5221 tttttattgg tttagctaca gttatttccc tagtaattta ctttttttca ataatggtta
     5281 gtagtattgg tggggcgttt ggtcacagca tatccccagg gatttttgga ttattatctg
     5341 gaatcatatt tgtttatatt agctataact accctagaaa aggaattttt actatttaca
     5401 caattgtttt attgttattt tttacgttaa tgggcggggc gtacttacct tggtttatca
     5461 gttcgattag tatggcgatc ttagctgata ttattctatc tgtatttgga tatgatcgag
     5521 caattcctca agttgtgtct tgggttctaa tgcaactagg cagtgctgcc ggacagtgga
     5581 ttcccatatg gttctttaca gatcgttttc gtcaagactg gatagataga ggacaatctg
     5641 ctgcaacaat ggatgcaatg atccactacg cggtgggtat atggggaatt atttcagtgt
     5701 tagttgtagc aagtttatct atgattggtg tattgattgg aagaaaagtc ttaaaaaaat
     5761 acaaaaaata gaacgtagtt taccttctat ctgtttgtac atctctaatt tacgctatta
     5821 cggaaagcat taacagttgt aaacgaataa gagaagaaat tgaatgcggg ggaggaaaaa
     5881 atgaaagtat acaaaaaatt atttagttat gtccctaaaa tgaaatacta cggatggtta
     5941 gccactttat tttctgattt ttctgtcgta ttaacagtct atggatatta cagtatttac
     6001 aaatttcttc agtctctaat tattgtagga gatgaatatt tagctaagag ttatgcggtt
     6061 caaactgttg cttggttaac tttaggagct ttattttata ttttttctgg cttattttct
     6121 catatccttg ggtttcgctt agagactaat ttgagaaaaa agggaattag cggtttaaca
     6181 gaagcaagtt ttcgtttttt tgatttaaat tcttcgggat atattcgaaa gacaattgat
     6241 gacaacgcgg ctaaaacaca tatggcaatt gctcacttga ttccagatag tgctcaagcc
     6301 atagtaacac ctgttttatc agttgcctta ggctttttca ttaactttaa aattggattg
     6361 gttctaatcg ccatgttagt aattggtgta ttcttgttaa agaaaatgac agagaataca
     6421 aattttattc aaaaatatca agaatcgtta gatgtgttaa gctctgaaac ggtagaatat
     6481 atccgcggaa ttcaagttat aaagatattt ggtgctaaag taacatcatt taaagccttg
     6541 aataaagcaa ttaacgatta tgctaagttt gcatataagt attctttgag tgggaaaaca
     6601 ccttttgttc tttttcaatg gctatttttt ggcatagttt gcattttgat agttcctata
     6661 acctttttta taacttctct taccactccc aagatcttag ctgttgactt gttgatgttg
     6721 ttttttttaa gtggaattat ttttgtttct ttcatgagaa taatgtatgt aagtatgcac
     6781 ttgtttgaag caagatatgc agtcgaaaca ctagactccc tttatttaga aatgaagcaa
     6841 gaccaactaa aatatgggaa cgagacacag attaaaaatt gtgacattgc atttgaaaat
     6901 gtcagctttt catacaaaga agagtgtgtt ttagaaaatt tcaacttagt tttagaacaa
     6961 aaaagaattt atgcattagt aggaaaatca ggaagcggaa agagtacgat agctaaacta
     7021 atttcaggtt tctacaatgt ggataaggga cagattaaaa ttggtggtaa gcctttaaat
     7081 cactattcaa aagacacaat tattaacaca attgcttttg tctttcaaga tacgaaacta
     7141 ttcaatcaga gtatttacga taacgtttct atagcaaatc ctcaagcgcc agcaagtaaa
     7201 gtatttgacg cgctaagatt agccggttgc gaatcgattt tagaaaaatt tcccgaaaga
     7261 gagcatacct taattggtag taaaggagtt tatctgtcag gaggagaaaa acaacgtata
     7321 gctattgcca gagcaatttt aaaagatgca aagattgtta ttatggatga agccagtgca
     7381 tctattgatc cagataatga gtatgaacta caaaaggctt ttcaatattt gatgaaagaa
     7441 aaaacagtta ttatgatagc tcatcgatta tccagcatta aatctgtaga tgaaattctt
     7501 gtcttgtcag aaggaaaaat agtagaacgt ggaaatcata atgaattaat ggctaaacat
     7561 cgagtgtata aacaattggt cactaattat gagaatgcaa atgattggag gcttgacaat
     7621 gaaaaatttt tataaaaaaa aatttgcttt aactgatcag ggcgcagaag ctttaactaa
     7681 agcttctatt tctagttttt ttgtatactg cattaatatg gtaccagcat tcattataat
     7741 gatgctgata gacgaattag ttttagaaaa tgcaaagccc cgttggcttt attttgcagt
     7801 atcgtttgtt actttgcttt tcatgtattg gttgttagat agggagtatg aaaacttata
     7861 taatagtact tataaagaaa gtgcgcattt aagagtgcaa attgcagacg atttgtcaaa
     7921 tttaccatta tcctattttt caaaacataa tttatcagat ttatctcaaa ctatcatgtc
     7981 tgacgttgaa ggtattgagc atgcgatgag tcatgcaata cctaaagccg gcggtatggc
     8041 tctgtttttc ccttttattt cagtgatgct tttggttggt aatgtcaaaa tgggattagc
     8101 tgttattttg ccaacgttat ttagttttgt cttaatcttg ttatcaaaga aatcccaaac
     8161 gaaagccaat actaaatatt acgatacttt gagagaaaac tcggaagaat ttcaagaaac
     8221 tattgaattg cagcaagaga ttaatagctt taatctatct aaaaaagttc aagacagact
     8281 tttcaaaaaa atggaagaga gtgaaaggat tcatttaaag gtagaattaa gtactttttc
     8341 agtcatggcc ttatcctcta ttttctcata tgttagttta gcagtagtca ttctagtagg
     8401 tgttcactta ctgttaacgg gtgaagtgac tatactctac gtcgttggtt acttactagc
     8461 cgcaataaaa ataaaggatt ccttcgattc tatgaaagaa gcagtgcttg aaatatttta
     8521 tttagctcct aaaatacagc gaattagagc tatgaaggaa accagtatcc aagagggatc
     8581 agatagtcct ctgaaatctt ttgacattga gttgcgagat gtctctttct catatgataa
     8641 caatacccca attttagatc atatctcttt tacggctaaa caaggagaag tgactgcgtt
     8701 agtaggtgct agtggttccg ggaaaactag tatcttgaaa ttagtgtcta gactgtatga
     8761 ttatgatgaa ggctgtattt taattgatgg gtatgatatt aaacgtgtat ctccagcttc
     8821 tttgtttagc aaaatcgcta ttgtttttca agaggtaact ttgtttaata caagtatttt
     8881 ggaaaatata cgtattggaa atagtcaagc gtcagatgag gaagtgaaaa aagcagctcg
     8941 tttagctaac tgcgaagact ttattgaaaa actacctgac ggttatcata cattggtcgg
     9001 agaaaacgga agttcacttt ctggaggaga gcgacaacgc ctatccatag cacgggcttt
     9061 tttgaaaaat gcgccaattt taattttaga tgaaattact gccagtttag atgcggaaaa
     9121 tgaaaaacgt attcaggaaa gtttaaatcg tttaatacaa gataagacag tactaattat
     9181 atcgcatcgc ttgaagtcta ttgaaaaggt aaataaaatt gtagtgatgg atcaaggaaa
     9241 actagttgat caaggtactc ataatgagtt gtatcgaagg tcagaaatat acaaaaatct
     9301 cataaagaaa actaaactat ctgagaaatt cgtctatgaa aaagaagctc aatcacgatg
     9361 atgaaatagg ctttaaatta acctacaatt tttgggatat agttataatt ctttttcaaa
     9421 aagaataatt ttattaaaca gcatctaatt ttgaatacac gaaa
//
LOCUS       DACEQH010000082         9055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.084, whole genome shotgun sequence.
ACCESSION   DACEQH010000082 DACEQH010000000
VERSION     DACEQH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.084"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            26..970
                     /locus_tag="IW833_002436"
     CDS             26..970
                     /locus_tag="IW833_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAQ1308504.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWKNGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            1038..2351
                     /locus_tag="IW833_002437"
     CDS             1038..2351
                     /locus_tag="IW833_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ1308505.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVISASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            2435..3337
                     /locus_tag="IW833_002438"
     CDS             2435..3337
                     /locus_tag="IW833_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAQ1308506.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSVSFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            3342..4994
                     /locus_tag="IW833_002439"
     CDS             3342..4994
                     /locus_tag="IW833_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1308507.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEKVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKADSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            5018..5884
                     /locus_tag="IW833_002440"
     CDS             5018..5884
                     /locus_tag="IW833_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1308508.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(5926..6117)
                     /locus_tag="IW833_002441"
     CDS             complement(5926..6117)
                     /locus_tag="IW833_002441"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308509.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCGKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            6147..6686
                     /locus_tag="IW833_002442"
     CDS             6147..6686
                     /locus_tag="IW833_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308510.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGADVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            6814..7167
                     /locus_tag="IW833_002443"
     CDS             6814..7167
                     /locus_tag="IW833_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308511.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            7634..8110
                     /locus_tag="IW833_002444"
                     /pseudo
     CDS             7634..8110
                     /locus_tag="IW833_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            8110..8304
                     /locus_tag="IW833_002445"
     CDS             8110..8304
                     /locus_tag="IW833_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308512.1"
                     /translation="MAETRPKGYPKLKEYTPNRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            8310..8595
                     /locus_tag="IW833_002446"
                     /pseudo
     CDS             8310..8595
                     /locus_tag="IW833_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            8609..8975
                     /locus_tag="IW833_002447"
                     /pseudo
     CDS             8609..8975
                     /locus_tag="IW833_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
BASE COUNT         2574 a         1795 c         2314 g         2372 t
ORIGIN      
        1 aatttagaaa ggagcaagca ttattatgat ggaaaaagac gcaaaaatct acgtagccgg
       61 tcatcgtggc atggctggtt ccgcaatcgt gcgcgagctg aaccgtcagg actacaacaa
      121 tattatcacc cgcacccata aggagcttga cctgtgccgt caggacgctg ttgaagcatt
      181 tttcgcacag gaaaagccgg attatgtatt tctcgctgct gcaaaggttg gcggtattat
      241 cgcaaaccag aacgctctgg cagacttcat gtatgaaaac atgattctgg aaatgaacgt
      301 catcaactcc gcatggaaga atggctgtaa gaagttacag ttcctcggtt cttcctgtat
      361 ctatccgcgt atggcaccgc agccgatgcc ggaaagctgt ctgctgacaa gtgagctgga
      421 aaagaccaac gaggcttatg cactggcaaa gatttctggc ctgaagtact gtgagtttct
      481 aaacaagcag tacggtactg actatatctc tgttatgcct actaacctgt atggacctaa
      541 cgacaactat catcctactc acagtcatgt gctccccgct ttgattcgtc gcttccatga
      601 ggctaaggaa gcaggtctgc ctactgttac ctgctggggt gatggttctc ctctgcgcga
      661 attcctatat gttgacgatc tggcaaacct gtgtgtgttc ctgatgaata actatagcgg
      721 tgacgagact gttaatgcag gtactggcaa ggaactgtct atcaaggaac tgaccgagat
      781 ggtagcaaag gttattggtt acgaaggcga gattctgtgg gataccagca agccgaacgg
      841 tacacctcgt aagctgctgg atgtttccaa ggcaaccaag ctgggatgga catataagac
      901 tgagttggaa gatggcatcc gtctgtccta cgaggacttc ctgaataacc cgatgcgtgc
      961 tgaacgctaa tttaccgagg ttgctgtgat ttttgaggat tgcagcagcc tctttcttat
     1021 aaaaaggaga gatcattatg aatattgttt tactgtctgg cggttctggt aagcgccttt
     1081 ggccgctgtc taatgacatc cgttccaagc agttcattaa gattttcaaa actgaagatg
     1141 gatatgaatc catggttcag cgtgtctacc gccagattaa gaaggtagat aacgacgcaa
     1201 ctgttacgat tgcaacctct aagacacagg tttctgctat ccataaccaa cttggtgagg
     1261 atgtgggtgt atctgtagag ccttgtcgcc gcgatacctt cccggcaatc gcattggcta
     1321 ccgcttatct ggtagatgtg atgggcattg atccagaaga gtccgtagtt gtttgcccgg
     1381 ttgaccctta cgtagaagat gactattttg aggccctgaa atctctgtcc gatcaggctg
     1441 acaaaggcga agctaatctg gtgctcatgg gtattgaacc gacttatcct tccgagaagt
     1501 acggctacat cattcctgag agcgcagagc agactgcttt cgtaaatacc ttcaaggaaa
     1561 aaccttctga ggctgtggca aaggaatata tcgcacaggg tgcactgtgg aacggtggtg
     1621 tgtttgtcta caagctgaag tatgtcatgg agcgtgcaca tcagctgatt gactttgtag
     1681 attatcagga tctgttcaac aaatacgata ctctgactaa gatttccttc gactatgccg
     1741 ttgttgagca tgaggacaag attcaggtac agcgttttgc tggtatgtgg aaggatctgg
     1801 ggacttggaa tactctgacc gaaggtatgg aggaatccat tatcggtaaa ggtgttatga
     1861 atgagaattg cgatggcgta cacatcctga acgaactgga tgttcctgtg ttggctatgg
     1921 gtctgcatga tgtggtcatc tctgcatctc gggaaggtat tctggtttcc gataaggaac
     1981 agtccagcta tattaagcct ttcgttgaca agttcgagca gcagatcatg ttcgcagaga
     2041 agtcttgggg tagttataag gttatggatg tagaagcaga gagcatgacc attaaggtta
     2101 ctctgaatgc tagtcactcc atgaattatc acagccacaa gaaccgtgac gaggtgtggg
     2161 tggttctggc cggtactggt aagaccatcg tggatggata tgagcaggtt gttaaggctg
     2221 gcgatgtgat caccatgagt gctggctgtc ggcataccat tatggcagat actgagctga
     2281 agctgattga agtacagctt ggcaaggata tcaatgttaa agataaacag aaatttgagt
     2341 tagagtatta aaaaacggag gagggctcgt tatacagaaa ccgtttttat tgaagccttc
     2401 cgcaaaggat tatctgtggg ggggaaccgc ctgaatgatg aatttgcaaa aggaattgag
     2461 atgtctcctc ttgcggaaac ttggaatgct ctacgcaccc ggatggaccg agctttgttg
     2521 tgtctggtca gagtatcagg ggatgacctt gacggaggtg ttgcaagaac acccagagtt
     2581 tctgggaacg catccgcaga ccaaaggaga attgccgatc ctgatcaaat tcattgatgc
     2641 gaaaaaggat ttgtctgttc aggtacatcc cacagatgaa tatgcaattg aaaaagaaaa
     2701 cgggcagctt ggaaagacag agatgtggta tgtactggat gccagcttaa tctatgggct
     2761 gcagaaaata acagaagaag ctgtgatccg tgaagcgatt gctcagggaa ctgttgagcg
     2821 ttatcttcag aaggttccgg tcaaaaagaa tgatgtgttc tttattgagg cgggaacgat
     2881 tcatgcgatt ggtgcgggtg cactaattgc agaaattcag gaaaattcca atctgactta
     2941 tcgtctctac gattatgacc gtgttgacaa aaatggtaaa aagagagaac ttcatgtaga
     3001 caaagcgcta gaagtagcga acttgaaagg gttgtcagaa ccgaaacagc cgcttcgtgt
     3061 tctaaaatat cgtgctggtg tagctgcgga attgctttgc agatgcaagc atttcgaggt
     3121 ctctcgtatg attgtcaaca ctgagagaag acagacggta cactatcgtg cggattcagt
     3181 cagcttccgt gtgttgctgt gtgtcaatgg ctgtggtacc gttcgatttg ataatgaatc
     3241 gttggacatc tataaggggg actgcctttt tgtgccggcc gattctactg aaatcagcat
     3301 tcacgggtcg attcagtttc tggatgtgag aggataataa tatgaattat aaagtagaat
     3361 atgagcgttg gctgaatcat gccaatgcag atattataga agaactgcgt tcctattctg
     3421 agaaggacat tgaggactct ttctataaaa atttgtcttt cggtactggt ggtctgcgtg
     3481 gtaccatcgg tgcaggcact aatcgcatga acgtacacac cgtaggtaag gcaagccaag
     3541 gtctggcaga ctatctgaat aagaactatg aagccgcttc cgtggtggtt ggctatgatt
     3601 cccgtatcaa atctgatgtg tttgcaaaga ctgccgcaga catctttgct gcaaatggta
     3661 ttcaggtgca cttgtggcca gaactgcttc ctgttcctac agtatctttt gcgactcgtt
     3721 atctgaaggc agatgccggt gttatgatta cggcatctca taacccttct aaatataacg
     3781 gctacaaggt atacggtgca gacggctgtc agattactac cgaggctgct actgcaattc
     3841 tggcagaaat cgaaaagctg gacatctttg ctgatgtaaa gatggatggc gatgcttcta
     3901 agattgcatc cattcagtcg gaagttctga ctgctttcat cgaagaagtg aaggggcagt
     3961 ccgtcctgtt tggcgaaaaa gtggacaaga atgttgctat cgtgtattct cctctgaatg
     4021 gtaccggtct ggtgcctgtt acccgcaccc tggcagagat gggatacagc aatgtgaccg
     4081 ttgtggaaga acagcgtctg ccggacggta atttcccgac ttgtccttat ccgaaccctg
     4141 aaattaagga agctatgtct ctgggtatgg agtatgccaa gaagtacaat gcggatctgc
     4201 tgttggccac tgaccctgac tgcgaccgtg ttggtatcgc agttaagagc gcacatggtg
     4261 actatgttct gttgtctggt aatgagactg gtctgctact tctggactac atctgttctc
     4321 agagaattaa gcatggaaaa atgcctgctg accctgtgat ggtcaagacc atcgtcacca
     4381 tggatttggg tgaacgtatc gcatccaact atggtgttcg taccatcgat gtgctgaccg
     4441 gctttaagtt tatcggtgag cagattggta tgttggaaaa ggctggtaag gctgacagct
     4501 atatcttcgg ctttgaggaa tcttacggct atctgtccgg tagctatgtc cgtgacaagg
     4561 acggtgttaa cgctgcttat ctaatctgtg aaatgttctc ttactacaag actcagggta
     4621 tcagcctgct ggataagctg aatgagattt atacaaagta tggctattgt ttgaacaccc
     4681 tgcattcctt cgagtttgac ggtgcggcag gttttaccaa gatgcaggaa attatggttg
     4741 aattccacaa gggtctcgat ataattggtg gtaagaaggt tattgaaact ttagactaca
     4801 gtaagggcct gaatggtctg ccgaagtctg atgttttgaa gtttatgctg gaagataact
     4861 gctctgttgt ggttcgtcct tctggtactg agccgaagct gaagacctat atcagcgtca
     4921 gcgcagagaa caaggaagcc gccgaagcta tggaagctat tatggctgct gaactggcgg
     4981 aaatgttcga gtaagaaaag aaaggagaaa tcttgtaatg gaggaactgt tttctaacgc
     5041 tttgaaatcg gtgaagtcaa atcgtgtgct gtacacggca tcgttctttg caagatcctc
     5101 tcttttgtat ttgcaggaaa ttggacagct gactgctctg aaggcacata catctaggcg
     5161 tacaggattg tcatcttacc tttgtttcct ggtagaagtt ggttcaggca cattggagta
     5221 taaggggcag aaatatacgc ttcaagtagg agatatggtg tttattgact gtttgaagcc
     5281 gtatgtccat gagtcatcag aagatgattt gtggactttg cggtggtgcc attttaatgg
     5341 cattggtatg acggaagtct acaaaaaata ccttgaacga ggtggtcaac cggtctttaa
     5401 aacatcaaaa acagataagt atgctgactt gctgaggagc cttcatatgg tggcagaggg
     5461 acaggagtat gtccgggata tgaaaatcaa tgttctactc agtgaactgc tgatgcattt
     5521 gatggaagat tcttggaatc ctgacaaagt gccgaatttc tctacaaaaa aaatggaatt
     5581 gcagaacata aaacaatatc tggatgacca ttacacggag aagattacgc tggatcagtt
     5641 gagtgcagag ttttttatca ataaacagta cttagctaag atttttcgtg aaacttacgg
     5701 tacaactgtt gtcggttatc tgaatcagaa acgaatcaca caggccaagc acttgctacg
     5761 ctttacaaat ctaagtatgg aagagattgc aacaaaaatt ggcataacag atgcaaacta
     5821 tttttccaga acttttttca aggtggaagg tgttcgtccc agcgaattcc gcaagatgtg
     5881 gtaataaaaa gccggcggtg ttgagccgcc ggtcagtacc tgtcgttaac ccgcattcaa
     5941 agctttaatc tcagctttga tttcagcacg aacacgatag taatcttcag tttcgatttc
     6001 ggtatccagc cgagttgctg tctttccgca gtcaggacaa cgctctggct gttcgtcggc
     6061 agggaagctg tagtggcagg aatcacaggt gtaaatgtga tcgtcacgta taatcatcac
     6121 ctaccaatcc agtgcttaga tttttcatgc aattactatt atatcgaatt ggtgattcca
     6181 caaaaatccg cttcttgata aatgtacgat tcagagtgat atatagtact gtactgccaa
     6241 agggaatacc acaacggcag aaatggatga ttcacatgga agaaattatg cagatgattt
     6301 tagggaacgc aaaattccag ctcggacagg tggtttcgac ccctgctgta ttggaaactt
     6361 tcaaccccct gcagaccgca aacggcattc ccgaagcctt gagcaacctt tggtttagac
     6421 aggaaattag tattgcggag atcgaggacg cagttctgga tgccaagaac ggcaaggagc
     6481 tagcaaacag cctcaaccgc atcaagcttt tccgcaagtt caccctcgac cgagagctgg
     6541 acggcatggt cagactgaag ggcgcggacg tctggaagaa catcagctac ctcaaggtct
     6601 acaccgccac gccgcctaag aaaccgaccc ccccaaggtt ccagtcgaac ggctggagcc
     6661 ttttcttctc tcataaatcg gcataatgta tacaaataca gctccaaatg attgtgtagt
     6721 atatgcctgt ggattgactt gatatagtcg agagactatg gcaatataca cacaaccaaa
     6781 ggggaaaatc ccaaaaacaa ggaggaaacg ctaatggcaa tgagcgaaaa ggcaaagaaa
     6841 tatttgaagg agatcaaggg tgcaaagacc atcccggagc tgaaggatgt ggagatcgca
     6901 atcaagcgag acggtatcct tgcttgggca gagttcacca agttgaacga ggcggtggaa
     6961 gagaagaagg tcgcccttcg caagaaaaag caggagacca gccttcagga gatccttttc
     7021 tgggcctaca aaaaggaatc agacaagctg ctgaagatga tggacgaggg agcagacaag
     7081 gatgccatcc agatgcaggt ccagcgttac gacagcatcg gccagatcat tgcagaggca
     7141 gacctcgaag atgagtatga ggtttgagaa tacagcctga accactaaga accaaatacc
     7201 gaaagggact tctgaaacag acagaggtcc ctttcttttg ctcactttgg cggtcaatta
     7261 tgaaccaaaa accagtcgaa tgattgtgta cattatggct ttcaaacgac ttgataataa
     7321 tcacttttag agtgatatat gtactacaaa aaacaaaaga ctggagaaaa caacagatga
     7381 gcgaatacag aatcatttat atcagcgacg aggacggcgg cgaggtggaa ggatgtggct
     7441 acacggctac gatggttatg atgcggtcag aaacttcttg gaatttgcct gcggcatctt
     7501 gaaaatcctg cgggtgcgat tcatcagaag agtggactga aatacaagtt agcagataga
     7561 gaagagagtc aggtttcgac ttggctctgt tctctgtcta ctggcagaaa tacattgaag
     7621 ggaggtgacg actgtggcaa ccagaggaag aaaatcaacc ccgacagcca tgaagaagct
     7681 ggagggaaat ccgggcaatc gcccactcaa tgcaaaagag tcaacgccgg aaaagaaggc
     7741 accgacctgt ccgaagtggc tggaaccaga ggcaaagaag gaatggaagc gtctggcaaa
     7801 gcagatggag gccatcggaa tcttgacaga tgtggatatg gctgcctttg ccggttagtg
     7861 ccaagcctac gcccgctgga aggaagccga ggaattcatt acccagcacg gtatcatcgt
     7921 gaaaacccta tccggctatt ggcagcaggt accacaggtg tttattgccc agacctatct
     7981 gaaaattatg aaccgatttt ccgagcagtt cggtctgacg ccttcgtcca gaagccgtat
     8041 cattgccggt gatggcggtg gtgtcggagc agaagatgcg atggaggctc tgttgggagg
     8101 tggtagctga tggcagaaac cagaccaaag ggctatccca aattaaagga atacacacca
     8161 aaccgattca tgctttcgga atgtcactac gacaaagcca gagcggatcg ggcggtgaac
     8221 tttatcggac agctccgaca caccaaaggc aaatgggcag gcaaccgctt ctggctcttg
     8281 ccttggcagg aacagatcat ctagtgtaat ctcgcttaac tgtattaacg gacgtagcga
     8341 ctttattaag agtgttcatg gtaggagcat tagttgcaac aatgattgat attaaatttt
     8401 tatggtacca acgattggcg atgtgacatt gaatatgcaa aactatatcg atatgatatt
     8461 atctaaaatc ttactcgctg ggatcgtcga ggtgtttatt ggttgttagg aaagaaaaat
     8521 atgacccaac tcgagcgatt tttatcgtat tacttgtgtg tggctttatc tgcattgtgt
     8581 gtgatttcga aataagggag agccaaaaat gagtagaaaa ttgatattga ttagtcacgg
     8641 aaaattttgt gaggaattga aaaaaagtac aaaaatgatt atgggacctc aagagaatat
     8701 ctacacgtta cctttattac cagaagatgg cactgaaaca tacagacaaa agctcttact
     8761 gtcatagatt cgttaaatga ttttgtggta ctatgcgatc ttttaggcgg tacacctact
     8821 aatatcattt ctaaactgat aatggaagga caacagatta aactttatgt cggattaatc
     8881 ttcctatggt cattgaattt atcaactcgc aagtgattgg ttcagagccc gagtttgttc
     8941 gtgcaggatg tgaaagcatt gtatctgtca actaattaat tagtggaata gtagaagatg
     9001 aagagtatat tatttgtcaa attaagcgag acaaaacttt ccatttacgt aactc
//
LOCUS       DACEQH010000083         8835 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.085, whole genome shotgun sequence.
ACCESSION   DACEQH010000083 DACEQH010000000
VERSION     DACEQH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.085"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            21..1157
                     /locus_tag="IW833_002448"
     CDS             21..1157
                     /locus_tag="IW833_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HAQ1308513.1"
                     /translation="METTEAVQDDQDLAKMYTPGVAELSLMIEKNHALARELTISGKL
                     VAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAIPLAIEQKSVDEIVQTIV
                     NLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEGTAIVVLAGLINAAKIQR
                     KTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRLKKQDSTLNKYQRELLDH
                     IHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDNPIIFALANPVPEITPDL
                     AKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKVDLSLQLEVAKQLAAKVA
                     EPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAENIK"
     gene            1138..2442
                     /locus_tag="IW833_002449"
     CDS             1138..2442
                     /locus_tag="IW833_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1308514.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            2468..3481
                     /gene="citC"
                     /locus_tag="IW833_002450"
     CDS             2468..3481
                     /gene="citC"
                     /locus_tag="IW833_002450"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAQ1308515.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            3485..3778
                     /gene="citD"
                     /locus_tag="IW833_002451"
     CDS             3485..3778
                     /gene="citD"
                     /locus_tag="IW833_002451"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAQ1308516.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            3783..4688
                     /gene="citE"
                     /locus_tag="IW833_002452"
     CDS             3783..4688
                     /gene="citE"
                     /locus_tag="IW833_002452"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAQ1308517.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            4681..6240
                     /gene="citF"
                     /locus_tag="IW833_002453"
     CDS             4681..6240
                     /gene="citF"
                     /locus_tag="IW833_002453"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAQ1308518.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            6237..6794
                     /gene="citX"
                     /locus_tag="IW833_002454"
     CDS             6237..6794
                     /gene="citX"
                     /locus_tag="IW833_002454"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAQ1308519.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            6787..7650
                     /gene="citG"
                     /locus_tag="IW833_002455"
     CDS             6787..7650
                     /gene="citG"
                     /locus_tag="IW833_002455"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAQ1308520.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            7884..8293
                     /gene="tnpA"
                     /locus_tag="IW833_002456"
                     /pseudo
     CDS             7884..8293
                     /gene="tnpA"
                     /locus_tag="IW833_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         3071 a         1382 c         1801 g         2581 t
ORIGIN      
        1 aaaatgtagg tgttttacgg atggaaacaa cagaagccgt gcaagatgat caagatttag
       61 caaaaatgta caccccaggt gtagcagaac taagtttaat gattgagaaa aatcatgctt
      121 tggcaagaga attaacaatt agtggtaaat tagtggccgt tattacagac gggtcggcgg
      181 tattaggtct tggaaatgta ggaaaccaag ctggattacc aatcgtcgaa gggaaagcgc
      241 tgctttataa aaatttagca ggtgtcaatg cgattccatt agccatcgaa caaaaatcag
      301 tagacgagat tgtacaaaca attgtcaatt tgcaaaatag ttttgcaggg atccatttag
      361 aagatattgc agcaccgaag tgttttgaga ttgaagaaaa attacaagaa aaactttcta
      421 ttcctgtgta tcatgacgat caagaaggaa cagcgatagt tgttttagct ggattgatca
      481 atgctgctaa gatccaaagg aaaacattaa ctgaacttcg agttttgatc aacggcatgg
      541 gagcatctgg ggttgcaaca gctagactat taatcgctgc tggtatcaaa aatctaacat
      601 tggtggataa acaagggaga ttaaagaagc aagatagtac attaaataaa tatcaaagag
      661 agttgttgga tcatatacat gatacaaata ataatgcaag cgttttggaa gatgcaatcg
      721 ttggacaaga tgtttttatc ggtctttcaa taggaaatgt tttaacgcct gaaatgattg
      781 gaaaaatgaa tgataatccg attatttttg ctttagccaa tccagtacca gagatcacac
      841 ccgatttagc aaaagaatct ggtgcaaagc taatcgctac aggttcttcg aaatatccta
      901 accaagtaaa caatatttta gtatttcccg gattgttcaa gggactatta ctatcgaaag
      961 cgaataaagt cgatctttca ttacaacttg aagtagccaa acaattagct gctaaggtcg
     1021 cagagcctac ggtagaaaaa ttcatcccta atgtttttga tgaaggagtc gcaaacgtcg
     1081 tttcacaggc agttttggat tatttacaaa aactaaaaga aaacgaggat aatatacatg
     1141 ctgaaaacat taaatagaat taaaattggt tttattcctt tatctcttta tatcattttg
     1201 gtactcgcgt atcttgcaat gacgataagc aatatcgttc cagaaaatga tatgttagtt
     1261 tctttgggta ttatgactgt ttttgcttat ttattagaag aaattggtaa aactattcct
     1321 attttaaaag atttaggtgg taaagtatta gttgttacat ttttgcctgc atttctagtc
     1381 tataaacatt ggctacctca aagtactgta actgtggttt ctgattttat gaataataac
     1441 aatttcttga tgttctttat cacactacta gttgtcggca gtattgcttc aatgaatcgg
     1501 caaacattgg ttaaagcaac ttcacgtatt atcattattt taattttatg cgatgtgatt
     1561 ggttctttga taggaacagg tgtgggaata ctccttgggc tatctgcttg gaatgcgtat
     1621 ttctttatcg ttgcgccgat tatggctggt ggtgttgggg aaggagcgat gccattatcg
     1681 attggttatg cagcaatatt atctggtatg ggacaaaatg atatctttgg gaagattcta
     1741 ccgtgtgtga tgttgggaag tttagtcgca gttctactgt ctggtatgct caaaaaaatt
     1801 ggagagaaat acccacaatt aaatggtcag ggtaatttga tcgatgggga tgatgaacat
     1861 ttagagaact tgaagggttc agctaaacca gtcaatattg aaaaaacatt gttagcaggc
     1921 gtggtagcaa taacgattta ttttgttgct gtctatgttg agcacttatt aaaatcactc
     1981 cttggctttt ctattccttc acctgtgatc ttattagtct ctcttatggg ggctaaaatg
     2041 cttggtttta tccctaaaaa tatcgaagaa ggtggaaatt cgttgtatgg attcactgtt
     2101 cgtgggatca cacctccctt actttttggc gtaggggttg caaaaacaga ttggttagag
     2161 ctgatcgaag tatttaaaaa tcctgcaacg atagtaacaa tcattgtgac ggtagtagct
     2221 attgtcgcta cagcttttat cgccgcaaaa tttttaaaaa tgtacccagt tgatactgcc
     2281 atcgcagttt catgttgtag cggacaaggc ggtacaggtg ctttagctat cttagctgcg
     2341 ggtgatcgca tggaattaat gccatttgca caagttgctg ttcgactagg gggtgctatg
     2401 accgtaacgt tcgctatctt tttaatgggt ctgctatcat aagaacataa agaaaggacg
     2461 tgaccatgtg tttgatcaaa cagatatcca acgattattt ttaaaaaata actatatcaa
     2521 agaccaatgg gaaaatcttt tgcttgattc aggaatacaa aagaagcaaa tcgaagattt
     2581 tcaacattta gatcaaacat tgggtatata ccataaagaa aagttagtag gaacagtatc
     2641 ttatcaaaac aacgtgatta aatatatcgc cgtgtctgaa aaatataaag atggtggaca
     2701 tacatttaat gtactagtct ctgcaataat tcaggcaatg agtcaacaag agatttttca
     2761 ttatattgtt ttcacgaaac cgcgctatca aaaaagtttt aactttttag gttttgaaac
     2821 aatcgtatct accccaaaaa tagctgtatt agaaatggga gatagttcta taaaacagta
     2881 cctagagagg attcctaaag tgaatggtga aaaaaaagca gcaattgtca tgaatgcgaa
     2941 cccatttaca gaagggcatt attatttagt aaaacaagct gccaataaca acgattgggt
     3001 atatgtattt gtactcgaaa aagaacaaaa tcttttttcg acgaaggacc gactcaaatt
     3061 agttcaacaa ggattacaaa aactgaaaaa tgtcattgtt ctatcaggtg gcgagtatat
     3121 tattagccct gcaacgtttc ccacctattt tttacgagaa gatgatgaaa tagcaaagga
     3181 gcaaatggtc gtagatgcta ctttattcaa agaaagaatt gccacttatt tagatatctc
     3241 tgctagatat gtgggagaag aaccactttc cccaatgact cgttcatata acgacatact
     3301 ggaaacgatt ttaccccctg aaataaaagt tcatgtcctt gcaaggaaga aaacggaaca
     3361 acaccaagtt atttctgctt ctcaagttcg taaagcgtat cttgctgggc aattggaaaa
     3421 aataaaatat atggttccag aaacaaccta tcaatactta aaaaataaaa gggagcgtta
     3481 agtaatggaa ataaataaat cagcgattgc tggaacatta gagtcaagtg atgtccaaat
     3541 catgatttca ccgaatacag aaggaataga aatcgattta gacagtcaag tgattgagca
     3601 atttggaaaa caaatcaaaa aagttatcta tgacacacta gcagtttatc agattaaaaa
     3661 cgtaaaactc aaggtagtag ataaaggggc attagattgc gttattgttg cacgattaaa
     3721 aacagcgatc catcgttcct taggtatcca acaccaagaa acaccgtggg aggtttaatt
     3781 agatgatgaa agaacgtttg agaagaacaa tgatgtttgt tccaggaaat aatccagcga
     3841 tgatcaaaga tgcaggtatt tatggtgctg actcgattat gttcgacctg gaagatgcag
     3901 tgtctatggc agaaaaagac agcgcaagaa tgcttgtggc tgaggcattg aaaacagtgg
     3961 attatggtga cacagaatta attgttcgaa taaatgacct gcactcagaa tttggacgtg
     4021 cagacatttt tgcaatggtt aaagccggcg cgcatgtatt acgtttacct aaaacagaaa
     4081 cctctgaaga tataacagaa gtagaaaaag tgattataga agcagaaaac tatcataatg
     4141 ttccgatagg gcgaacattg atgatggcag ccattgaagg tgcttctggt gtattgaacg
     4201 cacctgcgat cgccaaagca tctgatcgat tgattggaat cgccattggg gcagaagact
     4261 atgtgacaaa tatgaaaacc aaacgatatc cagatggaaa cgaattgttt tttgctcgca
     4321 gtatgatcgt tcatgctgca cgtgcagcag gcatttccgc atttgacact gtttactctg
     4381 atgtggatga tatggaagga tttttaaacg aagtacgttt aatccatcaa ttaggttttg
     4441 atggaaaatc ttgtatcaat ccacggcaaa ttccagtagt caatactgtt tatcagccgt
     4501 caatcaaaga aatcaaacga tcactagaca tcatctcggc aatcgaagaa gcaaaagaaa
     4561 aaggttctgg cgtaatttct ttaaatggaa aaatgatcga taaaccaatt gtagaacgtg
     4621 cacaacggac attgatgtta gcaaaagcat cacgattgat tgatgaggag ggaaatatcc
     4681 atgcataata aacaaagacg tagcattcca tcaagtgttt taacaaagta taaaaaagaa
     4741 ttccaaagtg tttcattatc gccacaaacg attcaacgtt gtgcaccaaa agtaacgact
     4801 gtaactaatc agacaaataa attgatgcac tcgatcaaag aagctataga aaaatcgggc
     4861 ttaaaagatg gaatgacgat ttcatttcat catcatttga gagaaggtga ttttgttttt
     4921 aaccaagtga tggccgtgat ccgtgaaatg ggcatcaaag aattgacctt agcaccaagt
     4981 tctttaacga acactatgaa tgagatggtt attgaatgca tcaaggacgg tacagtaact
     5041 catatcacaa gcagtgggat gcgcggcagc ttaggtgaat ttatttctca tggaggaatg
     5101 gacaatcctg tgattttacg ttctcatggt aatcgtccac gtgcaattga aagtggtgaa
     5161 ttgaaaatcg atgttgcatt tctaggagta ccaacatctg atgattatgg aaatgctaat
     5221 gcgactgttg gaaaatccgt ttttggttca ttagggtatg caaaagtaga tgcacaatat
     5281 gcagataaag tcattcttct aactgatcat ttagttcctt accctaatac gcccattagt
     5341 attccgcaaa cacaagtcga ctatgtcgtg gaagtagaag caattggtga tccgaataaa
     5401 atcggtgctg gtgctacacg gtttactaaa aacccaaaag aattaaaaat tgctgaaatg
     5461 gtcaaagatg tcattgttca ctcaccttat ttcaaagatg gcttttcttt tcaaacagga
     5521 acgggtggtg cagctttagc agtaacgcgt tacttagcta gtgaaatgga agaaaaacag
     5581 atcaaagctt cttttgcatt aggtggcatc actaaaccaa cggttgattt attggaaaaa
     5641 ggtcttgttg aaaaagtgtt agatgtccaa gactttgaca aaggagcagc tgaaggaatg
     5701 gcgcggcttg agaaccaaca ggagatcgat gcttcctttt atgctgatcc aagtaataaa
     5761 ggagcggttg ttaaccaatt agatgtcgct attttatctg cattagaaat tgacacaaac
     5821 ttcaatgtaa atgttatgac tggttcagat ggtgtgctaa gaggtgcgat tggtggacat
     5881 caagatgcag caacggctaa acttactatc atctcagcgc cgttagtacg tggccgaata
     5941 cctacagtag taaatgatgt tactacattg atcacaccag ggaaaagtat cgatgtatta
     6001 gtcacagaag taggggttgc tatcaatccc caacgtaaag atctaatagc tatttttgaa
     6061 agaattccac aaattcctgt ttttacaata gaagaattac agcaaaaagc agaaaaaata
     6121 gtaggcatcc cggaaccttt gcaattcaca gatcggatag ttgcctatgt agaataccga
     6181 gatggttcaa ttttagatgt cgttcgtcag gtgaaagaat ttgaagaaga gagatcatga
     6241 tgtttgatac tggagaaatt caagaaatcg aggatatttt gatccataaa gaacaacgtg
     6301 ctaacttgca aagcaacctg ctttccaagt attcaaatag ttcattgatg gtctttacat
     6361 tgaatattcc tgggccgatc aaaaataatc caatgattga gcaggtattt cgtcagggac
     6421 agcagtcaat tcagcaaatg tttttaaaaa aaaaggtgga aatgacgtac acaaaagaaa
     6481 tcaatttgcc aagtggattg gactggtacg taatagtaac agaagcacct ttgaatctca
     6541 aaaaatggtt ggtcacgtta gaagagcaag acgcattagg tagacttttt gatttagatg
     6601 tcttgttcaa aaaaggagag cagataacaa ttgtcagtcg ccaagctctt aatcatgggg
     6661 aacgtccttg ttttatttgt gggaaatcag caaaggtatg tgcacgtaat cgtacacata
     6721 ctgtgaaaga aatgcaaaag aaaatcgaac aaatagtagc cgattcctta aaaggagcat
     6781 tgaaacatgc atgataatat tcaaaaaata gcacaagaag cacaattagc gatgctttat
     6841 gaagttacat gtatcaataa accaggattg gttgatccag tagaccccgg gtctcatcaa
     6901 gatatggaca tttttacttt tctacaaagt agtgtagttt tgactcccta ttttgaagaa
     6961 tttatccaaa caggattgga ccgacaacat caaccaattg aagaaacctt tatggcgatt
     7021 cgacagattg gtttagaggc tgaaactgcg atgttctcag tgacaaatgg tataaataca
     7081 cataaaggtg ctatcttttc attggggatt tttcttgcta tttgtggccg gttaactatt
     7141 tggaaagttc cttgtaaaaa aagtttcttt caaaaagaaa ttcaaaaaat gacgaaaaat
     7201 ctacttaacg attttgggaa gattgatcaa acaaaaccaa aagcatggac ttggggagaa
     7261 tatctatttg ttaaccatgg attgacaggg atacgagggg aagccaaaag aggttatcct
     7321 tgtgtttttg accgaggatt gccttattat caagcatatc aagggtcgca acaagataag
     7381 atgatagaca ctttactttt tctatcaatg gaagtagaag ataccaattt gatcaaacga
     7441 agtaataata gacatgtttt tgaagagtat caattactta ttcgtcctta ttttgaatta
     7501 gggggtgtaa aaaccataca aggtaaaaaa tatttggatt acctaaatca gcaatttaaa
     7561 aagaaaaatt ggagtattgg tggaagtgca gacttattga tccttacaat ctttttagat
     7621 aaaatcatgg acaaaggatg gttatgttga tactataata cattattttg tattagtaag
     7681 ctaataacta aaaaatggtt caatacaaat attttataaa aaatggtagt gcctatcctt
     7741 tcagattgta gacaaagaaa aatttctttg tctacaattt ttttgttaat gacttcagtc
     7801 agtttcgtgc gcaaagcgtg cgaaacaaga aaaccatgcg tttctacacg tggaagaact
     7861 agcatatcga aaggagtttt ttcatgtcaa atgacgataa aagtttagcc catactcgtt
     7921 ggaattttaa gtatcatatt gtatttacac caaaatatcg acgaaaagta atctataggc
     7981 aattacgaaa agatattgga aaaatactta ggaagttgtg caaaataaaa aacgtggaaa
     8041 ttatagaagc acatgcaacg ccagatcata ttcacatgct tgttcaagta ccatcaaaaa
     8101 taagtatatc cagttttata gttatctgaa agatcgtagt gctgtgctaa tccacgagca
     8161 gcacgtaaat cacaaatata gatacggaaa taaatcattt tggtcggagg aatattatgt
     8221 gagtacagtc gaattgaatc aaaagacaat tgaaaaatac atccgtgaac aagaagctga
     8281 tgatcgcatt tgagatagta taagtaaaag agagtagatg ttgcaataac aaaagggtaa
     8341 aaatggacta cctccagtaa aataccgaaa aatagtttaa cttttggaga taagtccatt
     8401 taagggtcta tagaatgaca atttttttag gggattttac tattattact tcaagacccg
     8461 cttggattcg caaagtgagt gaactcttaa aactacctga tagtaggttg ttttaagagt
     8521 tatctgaaag taaaaattgc atttatattt ttgataaata cgtaaattta tatatacgta
     8581 tttatcaaag ttttaggcca aaggaatatt gtgtaaatac actaggattg aatgaataga
     8641 ccagaagaaa ttgcgtccaa gaacaaaaga agcatgatag tgctctatat aaattgggca
     8701 ttcaagaatc tgaaatttaa aggtagaatt ttagttagac ttttagattt taaacacaaa
     8761 aataaccata caagcaacaa gaagcaaagt gctatattat aaattgtccg tccgtttact
     8821 tacataagat cattg
//
LOCUS       DACEQH010000084         8493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.086, whole genome shotgun sequence.
ACCESSION   DACEQH010000084 DACEQH010000000
VERSION     DACEQH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.086"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..173)
                     /locus_tag="IW833_002457"
     CDS             complement(<1..173)
                     /locus_tag="IW833_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1308521.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IW833_002458"
     CDS             complement(593..1714)
                     /locus_tag="IW833_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAQ1308522.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /locus_tag="IW833_002459"
     CDS             2042..3451
                     /locus_tag="IW833_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ1308523.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IW833_002460"
     CDS             3448..4176
                     /locus_tag="IW833_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1308524.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IW833_002461"
     CDS             complement(4304..5215)
                     /locus_tag="IW833_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1308525.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IW833_002462"
     CDS             complement(5232..6239)
                     /locus_tag="IW833_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1308526.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IW833_002463"
     CDS             complement(6236..8365)
                     /locus_tag="IW833_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1308527.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IW833_002464"
     CDS             complement(8370..8477)
                     /locus_tag="IW833_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308528.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2682 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DACEQH010000085         8426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.087, whole genome shotgun sequence.
ACCESSION   DACEQH010000085 DACEQH010000000
VERSION     DACEQH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.087"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            472..2109
                     /locus_tag="IW833_002465"
     CDS             472..2109
                     /locus_tag="IW833_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1308529.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2254..2994)
                     /gene="truA"
                     /locus_tag="IW833_002466"
     CDS             complement(2254..2994)
                     /gene="truA"
                     /locus_tag="IW833_002466"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1308530.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3185..3820)
                     /locus_tag="IW833_002467"
     CDS             complement(3185..3820)
                     /locus_tag="IW833_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1308531.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3967..5208)
                     /gene="sstT"
                     /locus_tag="IW833_002468"
     CDS             complement(3967..5208)
                     /gene="sstT"
                     /locus_tag="IW833_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ1308532.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5406..6402
                     /locus_tag="IW833_002469"
                     /pseudo
     CDS             5406..6402
                     /locus_tag="IW833_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6418..>8426
                     /locus_tag="IW833_002470"
     CDS             6418..>8426
                     /locus_tag="IW833_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1308533.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2786 a         1547 c         1293 g         2800 t
ORIGIN      
        1 aaactaatca gaatcgtaca aatatttaat aaccaaatat atagaacata acttaatatt
       61 aagttatttg aaattccata aaaaacgact gcaggaatta ttgtaagaaa aggataaaaa
      121 tagttcagtc catctccata attgttaaat gttgtatagt tgattggccc ttcaaaaata
      181 gaagataacc ctttgattcg tgaaagatga aaagataaat cacctgttgt ataaatttct
      241 tgagcatttc tcataaaatt tactgaatat aaagtactaa gtaagataag aactactaaa
      301 agaaatgctt ttttacttat tttcatagat ttgttgttct ccttttttaa ttctattttc
      361 agtatactag aattgtcttt ttttaataag ttttgcgtta aatttttcat agcactagta
      421 tgatatacta gaaaagaatc aatttttatt acttttaaat aggagaaaaa aatgaaaaaa
      481 aacaaaaacc aaataataga tatcattttt atgttatttc taagttttgc ttacgtcatc
      541 cctctattca tgtctatggg aatctatcat agtgtcaatc aggatactta ctttcattta
      601 tctcgaatca taggactaga taatgtatgg agttctccag taaacttcaa taacttcgat
      661 catcatggaa caatgatgaa tattttttat ccatggctta ctctttatcc agctttttta
      721 ttttataaaa tgatgggaaa tcttgtttta agttataata tctactattt ttttatcact
      781 tttttaacaa tggtcgtttc ttatttttct atgaaacaaa taaaaaacaa tcgttatatt
      841 tcgctgttat tttcgattat ttatacattt tccgcctatc gtgccattga catattccat
      901 cgtgcttcat tgggagaagc tgtcgcttta acatttttgc ctttgatact aatggggtgt
      961 tacgaaattt atattagaga ttaccaaaaa tggtactggt tgtctattgg catgacgcta
     1021 gtagtgtata ctcatttact ttctgtcgct atggtttctg tatttattgg aggaacgttg
     1081 tttctttctt tttatttttg ggaccaaaaa atcgctagac tcttaagtct tttaaaagct
     1141 accgtcctca cctttttttt aagtgcggga ttcttaattc cttttattca acaatctaga
     1201 gcacaagaat tgaaagtccc actgggaaaa gaattaagtg gtatggctcc tagtgatatg
     1261 ctgacacaca ttttaaataa taactataac aactatacta ttggattatt cttatttctc
     1321 ggattgattg gtgcttttat ttttataaaa aaattaactg ctgatgattt atttattttc
     1381 tttcttggtg tatttgttct tttttgttca accaatttat ttccttggca actttttaac
     1441 catacaccag ttaagagtct acaatttgtc tggcggttaa atggtttttc ttctttattt
     1501 atagcctata cgatgagtat agtgatctat tatatttttt ctactaaaaa taattcatgg
     1561 aaaataatgg tctttacgtt tcttgcacta atattgcatc tctcaggagt aagcaattct
     1621 atccatgcta acaaagattc attaacactt atagcacctg aagacgctgt tgctatagct
     1681 gaaaattaca atcatactga ttatgcaaac aaagagtcaa tctatcatcc tgatatgatt
     1741 aataatgatc aatatttact aggtaaccag gaaataaatc caacaactca ttttatgagt
     1801 aacaaactta caattgaatt agataattcg aataataaaa atactgtctt gacgacacct
     1861 atttatcgtt ataaagggca agtggcttct attaatggca aactagtaca aacaaaatta
     1921 tctaagtttg gcacaactga attaactatt cctccaggta taaataaagt cgttattact
     1981 tatcagtaca caaaactagc aattgcttca agatatttat ctatcgttac tttgatttta
     2041 tttttacttt atcgatttac tttttctaaa caaaaacaac cgagaagaga agtacaacat
     2101 tctcactaga tttttattta agaaagactt tttagctgta cacataaaca ggctaaaaag
     2161 tcttttttta tatttcttta atttgcttca tacattattt aacatcgata tttatactgt
     2221 gaaacatgcg tttcatccat tatcaaagta tttttatcga taataaactt catccaaaaa
     2281 cagaccttgt gcagggacgg tttctcctgc atgttgccgc actttgcttt caaatatttc
     2341 atcgattgtt tctatcggca tggcacctgt gccgatttct agtaatgtcc ccatgataat
     2401 ccgcaccatt ttatataaga atccgtctcc gacaaaagta aaatgaagca tttctccctt
     2461 tcgttcgata gataattcat caatcgtacg cactgtggat ttttttgatt ttttcaatgc
     2521 agaaaaacca atgaaatcat gtgtacccac taatttttcg caagctgcat tcagcttgtc
     2581 catatccaac tcttgaggga aataaaagct gtggtttcgc tcaaatgctg aaggaatcac
     2641 gttgttccaa acataatagc tgtatttttt gcccaccgca ttatatcttg aatggaagcg
     2701 ctcaggcatt tcttcgattc ttttgatgac aatatcccgc ggaaggtaac gattcataaa
     2761 gtcgatcatt tctgccagtc caatttctgt gtttgtttta aaatttgcta cttgtcctcg
     2821 cgcatgggtg ccagcatctg ttcttcctga tccaataatt tctatctctt catttgtcat
     2881 ctgatgcaat actttttcga tttttccttg aatggtttta tcgtcatctc caagtctttg
     2941 ccatcctgaa tatctttttc cgtcatattc gattgtcaac ttgatgtttc tcatatttta
     3001 gtggctcctc tgtcttccat ttggtcttct ctctaccttt ttaagattgt agcacaaaat
     3061 atagctcctt tcttctctaa aacagaagct atacaagaaa actgtggaaa tatatatttc
     3121 agaaaaacaa aaaacagcca ccggatgaag tatccgctga ctgttcaatt tgacggctca
     3181 agttttactg tttgagatgc aagatactca accaggcaat gggcacataa ttaaagaaaa
     3241 aactgattct ttggcaaaaa cctattttat tcagtaatgg ccaagaattt atagaaggaa
     3301 ttcttgccag tccataaatc gccgcaaaaa gcaaactgat cacagacagt ccaaggtata
     3361 agtggcttac gtccatttgc ccattttttc gatagatcaa gaaaagaaga actggaaaca
     3421 ataaaaaacc actgtaacct aaacctgatc cgatattatg gacccatgtc gagacattcc
     3481 acgaagattc atgggtattg atactgaaca aacctgtaaa gatacaatca cctataccat
     3541 agaacgcgat ggctgctgta actagggtag ctaacacacg agatgtactt acaaatgcag
     3601 catacaatgc aggcaatgac aggatgaaaa agatccctga aaccacagac caaataagaa
     3661 acgcttttct tacaggactg gaaacctctc caaatacact gatcacgctc ttcatttgat
     3721 ctaattcagg ataatacaat cctaatacgt acggagtcaa aaaatcactc agcacgccta
     3781 ataataaaaa gaaaaaacca aatttacgta aaaaatccat gatacgcctc caataacaat
     3841 tacctatgct tttaattgtt attataagcg actttatctt gatttaacag aaacctctat
     3901 ttacacgaac atctatataa ctcattaaaa actcgaatca ttttaaaaat actaaatatc
     3961 taacgttcaa cgttcactta atccatctaa acaatctttc aatttgattt tttccccact
     4021 tcttttacgc gcgcctagct ctcctattgc agtaaacaac agatcacttg acgaatttaa
     4081 ggcagtttcc attgaatcct ggataacacc gataataaat ccaataccga cgatttgcat
     4141 cgcgacatca ttagagatac caaacaaact acatgctaat ggaatcaata acagtgaacc
     4201 gcccgcaaca cctgaagctc cacaagcagc taaagcagac aaaatacata aaaggacttt
     4261 caaaacaaaa ggtgcttcaa tccctaaaga atgtacagca gtcaacgtca tcaaggaaat
     4321 ggtaatcgcg gcaccgccca tattgatcgt agcacctaac gggatcgata tggaataaga
     4381 ctcttttgtc aatttcaatc tttcagccaa catcatgttt atcgggatat ttgcggcaga
     4441 acttcgggta aagaaagcgg gaagtgcgct ttctttcaaa caaaagaaaa tcaatggata
     4501 gggattttct ttaataaaca aaaacgtgag gaatgggtac acaactaatg cgacaaacag
     4561 catcgtaccg atcaaaacaa gtaaaagctg cagatactct gccaatccag ctattccagt
     4621 cgtagcaatt gattgataaa ccaatcctaa aatccctatc ggagcaaatg caataatcac
     4681 ttgtacgact tgtgacaacg ctatagatat ctgatccacc aactcttttg tcgattctga
     4741 acgtgctcga aaagctagtc ccaacaatac agcccagaat aagacgccaa ggtaattcgc
     4801 atcaataaga gattgaacag gattttgtgt gacattcgtc aacatagtag ataaaacact
     4861 ttccaaactt tgcggtgctt tttcagatac tgtttcttgt aaagtaatag gaacagtaaa
     4921 aattgaactt acaataactg ctacaagtgc agaaagcaat gttcccacta aatataagat
     4981 caaaataggc ttcacgaatg tcttattccc aattttatgc ttggcaattg acgccatcgt
     5041 caagaaaaaa accagaatag gtgcgattgc cttcaagcaa ctgacaaaca acgttcccaa
     5101 tattgaaata aatgtccatg tggggaaaaa gataccaaac aatattccga ctaaaatacc
     5161 aacaacaata cgttgaacta acgacatatc caaaaacttc tctaacaaat gaccaccctt
     5221 tcacaatttt aacaatcatt gtatcatttt atgcaaagaa actaaaggta taattttcta
     5281 aaaattctaa caatacaaac aaccatttct tgctaatttt gtactttttc tgtctacacg
     5341 ttgttttttg agatattcta agttaggatg ttattaatgg gcaacttatc aggaaacgtt
     5401 ttcttatgtt tttgtccaat ttttttgtga ggtgtttatt cttgaaaaaa ataaagaagg
     5461 agagttatgg cttcctgaca atctttgtcg cttgtataat gatggtttta cagcgtatat
     5521 tgatttcagc agtcactatt cctacatatg caacaaaccc tggtcgattc tacatctata
     5581 catttttaca aacttccgtt gttattggtg caaatttgat cccactttac atgggttacc
     5641 aatatcaaaa aatcgcccgt ttaaaagttt tacattattt aagtcgtttt gcctttatat
     5701 tcctaatagc cacactagtt tgcaatatct tttttttacg tacaagctgg cagcttgaat
     5761 attcgtgatt attggcttat cctaacacct atcagtcaga attactttac ttatgctgtt
     5821 tcttgtactt tgcttttttg tagtattcct tatacagttg gatttttaaa taaattatcg
     5881 aaaacaacaa ttaaatatct actattattt ttaacaattg gattagttgg ctgctctact
     5941 ctatttaata aagacccgtt tgcattacag aatggacaga gtattttatg gattttttat
     6001 ttatttttaa tgggttatgg tttgaaagaa tggaactgga aagaaaaggt ccgctataag
     6061 cttcctcaag tatttatcag cttgattgtt ttattcggtt tgattatttt gatgacacaa
     6121 atttcactca tcatcagagg aaatacagat acagcgctga ggttttctaa accatattct
     6181 ctcttcgcaa tgtattatac tttctcttcg tttttatttt tagaaatgct atctgataaa
     6241 ttaggtataa aaggaagagg aaattttctt gctgtatttt tgattgtcac tcaagttata
     6301 tgtaatttcc ctttagtttc ttatcgcgta tctacattta tgaaacgtga atttccaaac
     6361 tcagggaagg catggctgat aaatatcggg cagtttttta tttgctattt attagcagtg
     6421 tccctcctag ttatcttttt attttatata caaaaaatcc gtttcattca tcgtttcatc
     6481 gaatacttcg cttttgattc gattgaacaa ttaactcagc gttggaaaaa aagaattcat
     6541 tggctttttg ttcataaaag ttatttttta gttgcagcat ttttttactg ctttaccttc
     6601 gtgcagattt tcgttttaga accaaaagaa ggttggaaag aaactatcca ggttgcctta
     6661 aaaatattta ctcagcgaca agcacccatg atcctgacaa tcatcattat tatgggattt
     6721 ttcttcttac tattgttgat ttccaatcgt ttttggtacg ccttgactgc taccttgatc
     6781 attaacttac tgctcactat ttcgacagta atcaaaatgg aaatgcggga agagcctgtc
     6841 tttccttcag atttaaaaat actgaccgga ttgtcagaac tattatcgat ggttagccca
     6901 gtcttattaa tcgtgggcgg attgatcctt ctcctattat tgattactag tattattgta
     6961 caacgtagac tacaaaaaca gtattccttg aagattcatt ggaaacgtcg atttatcagt
     7021 atcgctgtat tattaggatg tttttcgggt gtctttttca ttaatcacaa aaattcgcct
     7081 tctttcttgc tgtttaattt atttaaagta aacaagactt tctttaacca gcaaggcgcc
     7141 gtaaaagata acggaccagt cattcaattc ctaaacaata tcgacattaa aatcatggaa
     7201 gaacctgctg gctattcgaa agaaaaaatt aatggaataa tgaaaaagta caacacaaag
     7261 gcagatgaaa tcaaccaaac aagaagtgac tggttaaaca atcagacgat cgttctcaat
     7321 ttaagtgaaa gcttcagtga tcctgcacgt gtccctaatc tgacagtacc tacagaccct
     7381 attccttaca tcaaaagcat tataacagaa ggaacaggag gattgatgtt atccgtcggt
     7441 tatggtggcg gaacagctaa catggaatgg gaaggactga caggcttgag tatcagtaac
     7501 ctgtctgctt cgttagttac accttatacc cagctagtgg agcgtcagaa aataagtcca
     7561 aatattaccg atctattcga tgaaaaaata gccatccatc cttttacagc tgctctttat
     7621 cgcagaaaac aagtgtttga taaatttggt tttgacaggt tctattattt agacagtccg
     7681 gataaattaa cttatacgga taaaatagaa caaagcccac gcatatcgga tgattcagca
     7741 tacaaagaaa ccttgaaagc tttacaaagc aatacagaaa cgtcacaatt catccaatta
     7801 tccactatgc aaaaccatat gccttatgat gattactata gtgaattgaa ctacactgct
     7861 gaaggaactg ctgtcattcc taatagaaga aaagaattac aaacttatat gcaaggcctt
     7921 cactataccg atacagcagt aaaaaaattc atccaagaaa tcgacaaaat cgataaaccg
     7981 attacctttg ttttttatgg tgaccattta cccgcaatct atagtgggaa tgattcgaaa
     8041 aaatatggat tagaacagca ccagacagat tatttcattt ataacaacaa gtatagtcgt
     8101 gaacaagcag ctacgttaaa taaagaagtt gttgctccta ataactttgg tgctatggct
     8161 cttgagcatg cgaatatcaa agtaactcct tactatgcgt tgttgacaga agtatctaat
     8221 catctacctg cattaacaat tgatcctaca gaaagcttgt ctaatcgttt taatggaaaa
     8281 caggtctttg ttacagagga tagtcaaatt atccaagaaa aaaatttaac tgagaaacaa
     8341 aaagaaattc tagaagatta tcgtctgata caatatgatc ttgttgcagg taaccagtac
     8401 agtgcaacat gggcgacaca aaaaat
//
LOCUS       DACEQH010000086         8102 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.088, whole genome shotgun sequence.
ACCESSION   DACEQH010000086 DACEQH010000000
VERSION     DACEQH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.088"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..2378
                     /locus_tag="IW833_002471"
     CDS             6..2378
                     /locus_tag="IW833_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308534.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2439..3908
                     /locus_tag="IW833_002472"
     CDS             2439..3908
                     /locus_tag="IW833_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAQ1308535.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3919..5928
                     /gene="topB"
                     /locus_tag="IW833_002473"
     CDS             3919..5928
                     /gene="topB"
                     /locus_tag="IW833_002473"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1308536.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            6274..6870
                     /locus_tag="IW833_002474"
     CDS             6274..6870
                     /locus_tag="IW833_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1308537.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6883..7782
                     /locus_tag="IW833_002475"
     CDS             6883..7782
                     /locus_tag="IW833_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1308538.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            7785..8102
                     /gene="ssb"
                     /locus_tag="IW833_002476"
                     /pseudo
     CDS             7785..8102
                     /gene="ssb"
                     /locus_tag="IW833_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184127.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT         3065 a         1351 c         1754 g         1932 t
ORIGIN      
        1 cggacatggc agaagaaaaa gaaccactag acaatagtcg cttgggaaaa tccaaaagaa
       61 aattagtacg attgcaaaat gaattaaacg aacaaattga gaagatgttt gaacaccaga
      121 gaaagaccaa cggtcagcca atgaacgata agagaaatgg gcattcttgg tttaggcaac
      181 aagaacgttt agaaaacaaa gtgcatagtc tgagagaaga gatcaagcaa caagaaaaac
      241 aggtagaaaa acttgaaaga caagaagagc taaaggaaat gggctataac aagtacggcg
      301 gcctggatat gacaatcgaa aatattccaa gaatcaaaga agaaatcgaa cgctttgaaa
      361 aaggtgaatc gactttttca gccgctacga tccgaaaata ccaacggaaa ttggaaacat
      421 tagaacagct gaaagaaaga tcagaaaaag gaaaagaaaa tcttcttcct gaagtacaag
      481 caattattga cagcggacga gtgacgcaat ggaaaaaaaa tccaaccatt tattttttga
      541 aagactatcg aaaagttgct ttagaactga atgaaaacgg gggatttgag gaatcaaaaa
      601 aataccaagc cacgtcggaa gaggaaaaag cagctgttcg tgaattactt gaagaggctg
      661 cgccaactga ttctttgaag gagaaagaaa acgatcattt ctatcgagta gaattcaacg
      721 aaaatgatcc agaaaaagga ttgaaaagtt atggtgggga gatcgtaaca ttagaattga
      781 tcgatcaact caaaacgttg gataaccaat acgccgcaga agcaggttat tataaattct
      841 attttgaaga agtgaagaac ggagaagtag tcgatcagat ccgaatggat ctaggggatg
      901 gcgtcaacag taatcaaccc atctatttag agttagcaaa attgtgcgaa caagtacctg
      961 aaaaggaaca aaaacaaacg gtcaaaaatg aacctgtttc gaaagaacaa atgcagtcga
     1021 aagaaacaaa aacaaaacaa aagggctatc aaaaacgaac ggcagaagag attaagcaag
     1081 aagtcaaatc cctttcgcaa caggcactgg atgcggtgaa aaagcatacc cattctcctg
     1141 aagacgtgaa agaacttttg aattttatgt ctcgttttcc agaacgatcc tttagaaatc
     1201 aattattgat tgaggaacaa tttccaggtg caacggcttg tttaggtcgt gctgcgttga
     1261 aaaaagaagg aatctatatc aaaaaagggg aacaagggaa caagatcttt gtcagaaaaa
     1321 cggtcaaagg attttatgag aaaggacgtg gctttgttcg agaaacagaa gctacaccag
     1381 aagataaaaa acgaatcgaa agtggtcaaa tcgaagtgat caaaaaacct tactacacga
     1441 ttgaaaaagt gtttgatatc acacaaacac agttaaaacc tgaagactat ccgaaaatct
     1501 ttccgaaccg tgtctttgat tttcaattgg ataaaaaagg acagtcagaa cttcaagctg
     1561 ggattcaagc ggtagccaac aatattggaa tacaaattcg agatatgaca gaaagtgaag
     1621 tctatcaaag agaattagga caagcacggg gggcgtatgt tcgtaacgaa tttactggcg
     1681 aagaagaaat cgtcatgaat actcgaaata ctcctacgca gcatttagct acaagtattc
     1741 atgaattagc ccatgcaaga atgcacaaat tttcagaatt agataccgca actaaagaat
     1801 tgcaggcaga aatgacttct tatattgtgt gcaaacattt tggaatggac acttcggaga
     1861 aagctattcc ttatattgca gcatggacga agaatggtca gacgttggat gataaggaag
     1921 cggcggaacg tgggaaaatc atgaatgatg tcagccgtgt cgcaaatgaa ttcattcaaa
     1981 ccatttctaa tgaaatcaat caatacagag aaagagaacc agagatccaa ccagcagaag
     2041 tgaagcagga gcataaaaat actgtagaaa cggcagagaa taagaatatt gtgcccgttg
     2101 gttcactttg gatcgacaag aaagacggga aagtaatgga agaagatacg ggtcgaacgt
     2161 tacatttgaa ggatgcggcg tttattgaag atccgcagca taatcaagtg attttaaaag
     2221 gaaaaagtta tggaaaagag attcaaacgg cgttaccttc agaagaattt gcgctaggaa
     2281 atccattccg attttcactt gaaacagtgt cagaagtggc caaacgaaaa aatgaaatct
     2341 attatgaaca acaattgtca caaaaagaac gcgtataaaa caagtaaaaa agactatctg
     2401 aaaaacggat agtctttttt cttttgggag gtttgaaaat gaccgaaaaa aaaatgagtc
     2461 ttattgatcg atgtaaacag atcgatatag tggattttgc tcgaaacaat ggaatggctg
     2521 tagtcaataa aggacgagac tatcgcttag aagaccatga ttcatttgtt tttgatcgaa
     2581 gaaaacaacg tttttattgg aacagtcaaa atattagtgg cgacattatt gaattagcta
     2641 aacttttttt catagacaaa gagatccaag actctaagca acaatttaaa gcagccacag
     2701 attttattct aaagaatgaa gacaaaacag aacgagtaga aaatcttcat tttgagacag
     2761 aaaagtacaa agatcacccc gttgattatc aaccactcac agaaaaagga aggaattatc
     2821 tgaaggaaga acgaaaacta ccagagtggc tgattgatta tgcagaaaaa gaagggttga
     2881 ttgctgaact aaagccgaaa catgaacggc aaaatttttt agttggagac gaccgtttag
     2941 atcatgcggt tgctttttta tggaaagatc cacagacagg agagacagta ggggcaagtt
     3001 atcaaggaac gatcgtcgat tttaatcgtt tcggtaaacg gggaacgtat aaacacattg
     3061 acaagaatcc tacgccaaat cacggattta atttaaaaat aggcgatccg aagcatttaa
     3121 aattctttga aagtagtatt gatctgttaa gttacgctgc attgaatcgg gagaaactac
     3181 aagatgcctg gttagtttcg atggacgggt taaaacatca tgtaatcagt cattacgttg
     3241 aagagtcgat ttctgaatta agacgaaaac aaacatttcc tcaatcgatt gaaatatgtg
     3301 tggacaatga tcgtgcagga catatttttt atgaaaaaga acagatgaag ggaatcgttg
     3361 atccttttac caataagaaa atccgttgtg agcgaggaat tccaaatgat tggcaagtac
     3421 ctaaagaata caaagctact tatgaagcag tcgcaaaaga aatgagtgtt gaacctgaag
     3481 caattatggc catacacaaa actgaaacta atttacagct gacaaatcaa ttggtgtctg
     3541 ctcatgatgt tcaatctacg tttggaaaga tgttggcgaa gggcgaacca gtagaaacaa
     3601 ttgatttgaa agaagcatgt accacggtcg cgaaggaact gaaagtatgt gaacgcgcag
     3661 atggaacata caactttgat cgtttttata gtagaaaagc caacataaaa gacgttaatg
     3721 caggaatcct tctttcctat aaagcagaac aatactacaa aggctataaa aagcatgaac
     3781 atgaatttgt acctgaagtg aaaaaggatt ggaatgatca attgaagcat gagatccagc
     3841 aacaagaaat cagaaagcaa aaacgtgcga tgttgtttca acaaggcaga caacaagaaa
     3901 gggagtgaag tcgtcactat gaaaaccgtc attttagcag aaaaaccgtc acaagcgaag
     3961 gcttatgccg atagcttttc taaagctacc cgaaaagatg ggtattttga gatccaagat
     4021 cgattgttta caggagaaac tgtgatcacg tatggttttg gtcatttagt cgagttagat
     4081 tcgcccgata tgtacgatga aaaatggaaa caatgggcat tagaacattt gcctattttc
     4141 cccaatcaat accactatca tgttcctaaa gataagaaaa agcagtttag tgtcgtcaaa
     4201 cgacagctgc agtcagctga tacgattgtg atcgcgactg atagcgatcg tgaaggggag
     4261 ctgattgcct ggtcaatcat tcaacaagca ggcgccaatc aaggaaaaac ctttaaaaga
     4321 ctttggatca actccttaga aaaagaagcc atttatcagg gattccaaca gttacgagac
     4381 gcagaagaga cctatccaaa atttgaagaa gtacaagcac gtcagatcgc cgattggtta
     4441 attgggatga acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga
     4501 agtttttctt taggtagagt ccaaacccca acactctata tgatttatca attgcaggaa
     4561 aagattagaa acttccaaaa agaaccatat tttgaaggaa aagcccaagt aatcgctcaa
     4621 aatggcgcat ttgatgcgaa gcttgatcca aatgaaaccc aagcgacaca agaagcattt
     4681 gaagcctatt taaaagaaaa aggtgttcaa ttaggaaaac agccaggtac gatccttcaa
     4741 gtcgaaacag agaaaaaaag tgcggctagc ccacgtcttt tttctttatc gagccttcag
     4801 tctaaaatga atcaactcat gaaagctagt gccaaagata ctttggaagc catgcaaggg
     4861 ttgtatgaag gaaaatatct aagctatccg cgaacggata ccccttatat tacggaaggg
     4921 gaatatgcgt atctgttgga tcacttagac gaatacaagc attttttaaa agccgaagcg
     4981 attccgactc ctattcatac accgaatagt cggtatgtga ataataagaa agtccaggaa
     5041 cattacgcga tcattcctac aaaaacggtt atgacagcag cggcttttga acaactaagt
     5101 ccactgcagc aggctattta tgaacaagtg ttaaaaacaa cggtggccat gtttgcggaa
     5161 aagtatacct atgaagaaac aacgatcttg acgcaagttc agcagcttca gttaaaggca
     5221 atcgaaaagg tgccgctaga tctaggttgg aagaagttat ttggaaaaga gagcgacgga
     5281 aaagagaagg aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggtg
     5341 gatctacaag ttcttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg
     5401 atcactgcaa tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg
     5461 aaagaagtgg aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa
     5521 caaaaggaat atatcaaggt agagaaaaat aagctcgtgg tgacgaataa agggattttg
     5581 ctttgccaag ccgtggaaaa agagccgctg cttacgagcg ccgaaatgac cgccaaatgg
     5641 gaaagctact tgttaaagat cggtgaacga aaaggaacac aaaccacttt tctagccaat
     5701 atccaaaaat ttgtctctca cttattggaa gtcgtaccag ggcaaatcca atccactgat
     5761 tttggatcga cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat
     5821 ttaggaattt gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct
     5881 tgtacgtctg aagcctgtga ttttaaaagg tcagaagggt tcatttgaag caacgattgt
     5941 tttaaaagaa gacttcacta caggctttga attttctgaa aagaaaaaaa cgaacttcaa
     6001 gaaaagaaca cgccgcactg caaaataatg gagggttaat cttatgcgta ataccacaca
     6061 caaacaacaa gaaatcgaaa atcaaaaagc caaaattcgt tcagaacagg aaaaaatgcg
     6121 tgccagcaaa cgaagactgc agcaattgaa gaaccagcag aggattcaaa aagaaatcga
     6181 attagatcga taaaatcttc ctaactattg tatacgtata aacttatgtt atactgtttt
     6241 tataagttta tacgtataca aaggagggag aaaatgacaa aagcaatggt atacaagctg
     6301 ctagaagaat ttaatggaac tttggctttg aaggatgcca aaaaagcggg catttctcct
     6361 gtaacgatca aacgtatggt agatcgaggg gagctggatc gagagtatcc aggttttttc
     6421 accttacctg gtcaatttcc tgatgaattg tttatggcgc aaaaaaaata tgaacgcggc
     6481 attatttcac atattaccgc gttagatttg tatgatttaa cagatatgat ccctagacag
     6541 atcgatctga ctgtacctta cggttatcac gtttctgaaa aaggattgaa ggaattcgca
     6601 gttgagctac actatacaaa agcggaatgg tatgaattag gtaagattga aataaaaagt
     6661 cgctatggga atcctattat agcttatgat ccagaaaaaa ccttgtgtga catttggaat
     6721 ccctggtata acgttgagga tgaaatcaaa gtaaaagcaa tcaaaaacta catggagtcg
     6781 gacagaaaaa atcttagaaa attgaatgag tatcgacgaa ttttgcctac agataaaacg
     6841 atgcgctctt atattatggc tttaaattaa aggagaatac acatgacacc tacgcaatta
     6901 aaagcaaaag tgaagaatat ttctagagaa aaagaagttg atccgcagct tgttttaaga
     6961 cattttatga tggaaaaatt cttggaaaaa atctcagagt ctccttatcg tgaaaatttt
     7021 gtgttaaaag gcggcttctt aattggctca aagtatggca ttgaaaatcg aacaactaaa
     7081 gacattgata cgactttacg ggaaatgaaa gtaaccaaag aaacattgac aacggttcta
     7141 aacgatattt tttcaacgcc gacgaaagaa ggcattcaat ttgagattca aggaatgaaa
     7201 gaaacgaggg aagctgatta ttatccaggg tttagcttac gtgtactggc acatttggaa
     7261 aacatgcggc cagattttaa ggtagatgtt actacaggtg actcgatcta tcctgcgacg
     7321 atcactcaca gtcataaatt gatgtttgag gatcgaacga ttcctttgga atcctatcct
     7381 acagaacaaa ttattgctga aaaattaagt gcaacctttg attttttaac agataattcg
     7441 agaggaaaag atttttatga tctctatact attcctaaaa tggagaagat cgatgagaag
     7501 accttgaaag atagtgtgag aaatacattt acacgtcgag gaaaaactga tccgttaaag
     7561 atatactatg aaaaggatat ggatgtactg aaaaatagtc acgagctaaa agatacctgg
     7621 aagaaatatc aaaaaagcaa tccttatgct gcgtcgatca cgtacgatga gacaatggat
     7681 tcgattagta atttgatgga aaaagtgatt cacgtcgaag aaaaagagcg aaaactagct
     7741 tatcaaagac accaacagat gaatagagaa atggagagat aaagatgatt aataatgtga
     7801 cattagtagg acgcttaacc aaagatcctg atttacgtta taccgcaagt ggtactgcag
     7861 tagcgacttt taccctagcc gtcaaccgaa atttcacgaa tcaaaacggc aattgagaag
     7921 ccgattttat taactgcgtg atttggcgca aaccagcaga aacaatggcg acattagcta
     7981 aaaaaggtag cttgattggt gtagttgggc ggattcaaac gcggacttac gacaaccagc
     8041 aaggacaacg tgtatatgtg actgaagttg tagcagataa ctttcaacta ttagaaagta
     8101 aa
//
LOCUS       DACEQH010000087         8056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.089, whole genome shotgun sequence.
ACCESSION   DACEQH010000087 DACEQH010000000
VERSION     DACEQH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.089"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(275..1174)
                     /locus_tag="IW833_002477"
     CDS             complement(275..1174)
                     /locus_tag="IW833_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1308539.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
     gene            complement(1195..1467)
                     /locus_tag="IW833_002478"
     CDS             complement(1195..1467)
                     /locus_tag="IW833_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAQ1308540.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            complement(1490..2896)
                     /gene="lacG"
                     /locus_tag="IW833_002479"
     CDS             complement(1490..2896)
                     /gene="lacG"
                     /locus_tag="IW833_002479"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ1308541.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            complement(3038..4744)
                     /locus_tag="IW833_002480"
     CDS             complement(3038..4744)
                     /locus_tag="IW833_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1308542.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            complement(4744..5061)
                     /locus_tag="IW833_002481"
     CDS             complement(4744..5061)
                     /locus_tag="IW833_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1308543.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            complement(5089..6069)
                     /gene="lacD"
                     /locus_tag="IW833_002482"
     CDS             complement(5089..6069)
                     /gene="lacD"
                     /locus_tag="IW833_002482"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1308544.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            complement(6072..7004)
                     /gene="lacC"
                     /locus_tag="IW833_002483"
     CDS             complement(6072..7004)
                     /gene="lacC"
                     /locus_tag="IW833_002483"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAQ1308545.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            complement(7015..7530)
                     /gene="lacB"
                     /locus_tag="IW833_002484"
     CDS             complement(7015..7530)
                     /gene="lacB"
                     /locus_tag="IW833_002484"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAQ1308546.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            complement(7547..7972)
                     /gene="lacA"
                     /locus_tag="IW833_002485"
     CDS             complement(7547..7972)
                     /gene="lacA"
                     /locus_tag="IW833_002485"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAQ1308547.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
BASE COUNT         2465 a         1677 c         1395 g         2519 t
ORIGIN      
        1 ataaaaagtg tggctaattt gaggcaaaat attaaattaa atttgccaca aattaatggc
       61 tccactatta ttttaaatca agaagtagta aataatctta ttttttacta ccagactgtc
      121 tttagatatt agcttttcgt cacccactac agttgacata gacccacaaa aaacttaaaa
      181 accttgcgat gtaagactgg cacggagaag accaatctat catcacaagg taagagtatt
      241 caaatcttat tgaagactca aataataaca aattttaatt aaggatagta atatcataga
      301 tttttctaga agtttcttga ggtgaaattt tcgttacctg tcttttatct tctaagaaat
      361 taccttcttc aagtgaagtg gagagaccac tccaaggttc caaagcaatg aatggacttt
      421 tattagttgt agaccacaga atcaagttcg gaaaatcatc aaaatctact cgaagacctt
      481 taccagactt ccttgaccga agcgttacgc tacgagactt gagctggtca agtgtaatgg
      541 catcatggga aaaaagtgag tagtccaaat cgagaatttt ttgattttcc aaaaaaggtg
      601 tccgatcttg caaatctaat aatcctgtct caggaaaact ctttggtatc gagcaagact
      661 caacctcact aaattcaata taataatcct catagttttc accttctact aaaggactat
      721 tgaatgctgg atgggctcca ataaagtaag gcattgtttt ttccatttcc aagttcgtta
      781 cctgaaattc ggttctaatt gatttaccat tcaaggtata aacaactctc agttcaaact
      841 gatataggta attatcaagc atctcagcat ttggtttaat actaaaggtc acgctattat
      901 cattgacttc ttccagaata aattcttctt ttcgaacaaa accatgtcta cgaataagac
      961 ctgtaaaaaa gggtctttct tgaggtctat aaatagccca atcatttcgt aaactaccac
     1021 agataggaaa tagaataggc gcctgtccat tccaataatt tggatcagct tgccagagat
     1081 actctatccc atccttgtct ttaatcgaag tcagttgacc accaagtgtt ttaaactgaa
     1141 cagtaagata ctcgtttttt aattcgatag tcacaactaa ttcctttctt tttattattg
     1201 gaaaggcaaa taatttacct cgaagtgaat agtctcacgt atttctacat cgagtgattg
     1261 taaaaattcc cattcaaatg ctgataactc taaatcagaa tggcaaatcc actcttttcc
     1321 agttgaaaca taccatttac cacattttag caacggggta gcttcaagct caggaatcat
     1381 aggacaatct tcgaaactaa caatatatac cttttcaaag cctggtctaa ataagtgttg
     1441 tactttttct aaagtatcgg ataacaattt tttacatttt caagtaatat tactctatca
     1501 cttgagtttc tgctactttc ttataccaat gagctgattt cttaggatag cgttcttgcg
     1561 tgtcaaagtc tacatagaat agtccataac gtttttcgta tccgtttgac catgagaaga
     1621 catccataag tgaccagatg aagtaacctt taacatttgc accatccgca attgcgtctg
     1681 ataaaacttc caagtgtttc ttcacgtaat caatacgacc atcatcataa acggtattat
     1741 ctacaaactc atctttgtat ccaagaccgt tttcagtgat gtaaatcttc ttgtagtttg
     1801 gataatcatt tttcactcgc atgatttggt catacaagcc ttcaggataa ataatccagt
     1861 cccaatccgt tctaggcaca taatctggtg cgattcggcg accaacacct ttaatctggt
     1921 acttagaact tcctttttca cccttaccat tgtgaatgat ttcagtctca ccatcaaagg
     1981 cttgcatcca atcactcatg tagtagttga taccaaggaa atcgttcaaa tctttagctg
     2041 cttcaagtgc ttggaagtct tcatcacgaa gatcaagttc tccaccattt tcagctagga
     2101 tgtggttgac cccttccatc gttttatctg aatagtgtcc aaggtaggtt gcatccaaga
     2161 taaatttgtt atggatgata tcttctaact cagcggcacg aacatctgct ggattttctg
     2221 gatcataagg gtatttggtt ggcaacgcat gtactacacc aatttcgcct ttataacctt
     2281 tatctttata caatttcaca gcacgtgcat gagaaaccat catattgtgg tgtgattgga
     2341 agactttggc aagatcatat tgaatgcctg gagggaattt accaaccaag tattgaccat
     2401 ctccaattgg gccaatttca ttgaaagttg tccagtagtt tacttctggg aactcttcaa
     2461 aacagaaagc ggcataatct acaaagtgtt ctatattttc gcggtttaag aaatctccat
     2521 ctgagtgaag aacttctggt gtgtcaaagt ggtgcaaagt cacgaatggc tcaacatgac
     2581 gtttgtggca ctcggcaaaa agattatggt agaattcaac acctttttca tttacttcgc
     2641 catagccagt tgggaaaata cgagaccagg caattgaaat acgaatacca ttaacgccat
     2701 atttttcagc taattctaaa tcaacaggat atttatggta aaaatcactt gcaggctctg
     2761 ctgtgtacca ataattgtct tcaagatact tatcccatgc aactgggcct ttaccatccg
     2821 tgtgagtcgc accttctgct tgataagcag ctgtagcacc accaaaaata aaatctttag
     2881 gaagtgtttt agtcataact ataaatcctt tcaaaaaaag catttgagac gatattctga
     2941 gttccggaaa ccaaggaaat gaaatgcaat gatatggtta acaatattat aaggaattga
     3001 aagaataatc tcgtctcaaa taacttttac aaatttatta atcaaactgt tgttgaacaa
     3061 atgcaagagc accttgtcca tcacgtgtca atttgatata ttgagctcct tcagtttttg
     3121 caagtttaat gcccaattta tccgtttcag ccttcatatc atcgaagttt gaagcgacct
     3181 gtggagccaa gataaccaag tcaaactctg gcaacatttc acggtgagca ccgtaactac
     3241 ccgctgccgc tttgactgga acattgtatt ctgctgctgc tttgttcaag gcattggcaa
     3301 gcaagccact tgtacctcca cctgcacaaa gaacaaggac attagtttct tttgtgatat
     3361 tattgtttgc tgctacatct tctttagcaa cgcctgcttt tccaagaacg gcatctgctt
     3421 tagcagtatt gaagtttgca gctacttttt ctttaagagc atcgtttgtt ttaccagaac
     3481 gttcttcttc aagaatttgt tcatcgtata ccttaacaaa tgggtaataa atgatagtat
     3541 caacaactac caagagtccg gctaagataa atgatagaac ttggaagtta gtaccgagta
     3601 caattcccaa cggaccagga gtaacccatg gaaggttggc agagaatgag ttcatgttca
     3661 atgtgtcaac aaagaattta aagatccaaa cgttgacaat cggagcgaaa atgaacggta
     3721 caaagaaaat cggattcaat acgattggtg caccaaataa gattggctca ttaaccccaa
     3781 agaatgttgg aacaactgag gcacgtccga tggcacggtt acgttctgat ttacaaatcc
     3841 acatgaacaa gaatggaaca atcaatgtag ctcctgttcc acccatggta acaataaaca
     3901 tttgagtacc agaagtgata actttgtctg catgttgtcc agcttggata agatgcaagt
     3961 tggcatcgat attggcataa gtgatagcag cgattgctgg ttcgacaatt gaaggaccgt
     4021 ggataccaac aaaccagaag aaggcataag caccaaagat aagtgtaatt ccaaggtaac
     4081 catctgcagc tgagaagaga ggagcaagaa gggtaccgat tgattctgca acggttacac
     4141 caagagttcc cttaacaatg agttcaaatc cataaaggag aacaaccgct acagtgaatg
     4201 gaatcaagtc tttaaatact tgagagatat ttggtggaac ctcttcaggc atacgaatag
     4261 taacattatt tttcacacag accttgtaaa cattaactgt cacaaaggct gcaataaatg
     4321 ctgtcaaaag accttttgtt cccgtgaagg ctgtcaagaa gcctccttct tttgcaggct
     4381 cagctgccat caaaaggaac cctaccatag acgctagcat cgtagataag aagttaatct
     4441 gattggtcgc aggtaggtca cggttcattg agtccgtcaa agctttagcg gtagtaccac
     4501 caacaaagaa tgctaggata cccatcgaat agctataagg agtcatcaag aaggtctcaa
     4561 tgtcttttga ccagtggaat ccccaagcat ttgggacata ggcaataagg ataaagatag
     4621 atgagaaaag gatgactggc ataccagcga taaatccatc acgaatagca cgaagataga
     4681 tgtttcgaga gattttctca aagaacggtt tccctttctc aataagttca atgagtttat
     4741 gcattatttt gctcctcttt tataaagttc gatgaggtga tgaatcacat cttttaataa
     4801 gacagtagtc attaagtggt cctgaccatg catcatggta acgctgtatg gaagttcctc
     4861 cccagaagct tctcgagcca acatagctgt ctgagaattg tgagcctctg caatacagct
     4921 tcctgcttct actacaagac tatctgcctt agcgaaatca ccattttcag ccgctttaag
     4981 cgcttctaaa agtttagagc gagcatctcc agcataagca acaatttcaa accctaagag
     5041 agtcatctct tctctgttca taacaatatc ctccgtttta tgtttttact atactttatc
     5101 agtccatgga ctagctgttt taacaagaac tttgttaagt tcatcaatat tttcgaatcc
     5161 agtagtacgc aaccattcac gtgctgcttt ttcaccttct ttgatgtaag gttctacaga
     5221 tcctgcccaa gtagcacgtc cacaaagcac accattaaac ttggcacctg agtcgtgagc
     5281 aaattgcaat gtttcttgga acaatttagc agatacacct gcacttaggt agatgtatgg
     5341 aaggttagtc gcttcttctt gtgctttaaa gaagtcagca gcttcagctt tactgtaaac
     5401 tacttcacca tcagcaaaac cttcgacata tttaacgtta actggtactt ctactttaag
     5461 aacatcaatg ttaaagcgtg gatcagagaa aactttcatc gcttcaatga ctttgcgagg
     5521 tttcactttt gcatattcaa cagaacctgc atctgagatt tcttcatcat aagcaaggat
     5581 ttccaagaag aaaggaatat cttcagcgac acattcagaa ccaacacgtt caatataagc
     5641 ttgtttttgt tggttaagtt catctgagct gtctacatca tagtaaagca agaacttaac
     5701 ggcatcagcg ccttgctctt tgatacgttt tgcagaccaa acatccaagc agtcaggcaa
     5761 acgttttgta cttgatgtat cataacctgt tttttcatag gcaagaagaa gacctgcttc
     5821 tttatccaat gcttttgttg ctggaagtcc atattcaggg tcaagcaaca ttgaagaagc
     5881 gtattttgta agttcatcag caaccaaaac ctttagttct tccatttgag ctacagttgg
     5941 ttctgtatct tgatactgtg ccatcaaacg tttcaaagca ccacgttggt caaatgccaa
     6001 agctgagata acaccgtttt tatctgaaat tttttccaaa gatttgcgtt tctgttctgt
     6061 aagtaccatt tttatacctc ttttactcca atttgtgagt ttagtgtttc gtaattagtc
     6121 atattcacat gccctgtcat tgtttcttga gcatttaaca tacccaatgt catcgcatgt
     6181 tttaataagt cagcgtcact ttttttacta tttaaagctg atgcaatacc agctaccgtt
     6241 gagtcccctg atccgactgg attaacaact gggatatcag gaatatctac cttatagaaa
     6301 acatcaccat gttttgcaaa ggcaccgttt cgacctaatg agaccactat ccattcgata
     6361 ccactaaaaa gtgactcttt aagaacgtct ttgagttctt caatatcttt tgttacttct
     6421 tttccaagca actgtgaaag ttcctcatta ttaggtttga tggcagtagg tttagccgac
     6481 gattttagaa ctgtttcaag aggtgcacct gaacaatcca aaacgactgc tactccttca
     6541 tccgaagcaa gttggataag tttttcataa taatcgttgg gaagtcctga aggtaaactt
     6601 cctgaaattg tcacaacttc tgattgttta ataagattgc tatagtggtc aagaaagcct
     6661 tctgcttctt catgagaaat tgttggtcct tgttccaaaa tttctgtttg gttaccctcg
     6721 tgtaagatag caatacagtt tctagtgttt cctgagattt tataaaaagc tggactaaca
     6781 ggagattgtt ctaattcgct ttcaataaat tcaccaattt taccacctaa gaaacctgta
     6841 gcagtaactt tatcaccaga ttcatacaag actctagtaa catttaagcc ttttccacca
     6901 gctgtcttac taacatcttt caccctattc actgtatcaa ttttcaatgt ttctagagga
     6961 taagagatgt ctactgaagg atttagtgtg actgtcagaa tcatttgtta taccttaatc
     7021 gtggtagtca ccacggtccc atctttcaag gaattctgta aagaaatctt catctgcttg
     7081 atgtgcatta tgtgtttcaa cgtgttcgat ttttgcaatc aattttttat tttcttctgt
     7141 tggtttgtac tcagcttcaa tgaaagcttc aatgatgtca ttcataagaa gaccaccagt
     7201 aatctttcca ccgaaaccga taacattcgc atttaactct tcttttgcat atatagctga
     7261 tgtcatatca cgaaccaacg ctgagcgaac acctggaact ttattgactg cattgttaat
     7321 gccgacacct gtaccacaga tacatactcc caagtctgct tgaccactta ctactgcttc
     7381 accgactttt ttaccataga tagggtagtg agtacgtgcg tggtcatatg tcccgaaatc
     7441 aaggacctca tgtccttttg atttcaagaa ttctgatact gccatttttt catctgtgac
     7501 aatgtggtca catccaattg cgattctcat tccttaactc cttttcttaa cacattttgt
     7561 taagcatatc tactcggatt tgatgacgtc cgccatcata tttaccattg acgaaacctt
     7621 tagcgatgtt tttagcaagc tcatcaccga cgatttcagc gccaaccgta atcattcgtg
     7681 aattgttatg tccacgtgtc atataagctg agcgttcatc tgaaacttct gctgcaacca
     7741 tacctttaat tttagttgct accatgaatg gaccagctcc ataagcatca attacaatac
     7801 ctaagttttg ctcatcttta tttacttcag aggcaactgc caaggtaaca tcaacaaaat
     7861 cttgtccgtc cttagtcaca tcaattacct caaaaccttc ttctacaaga aaatttttca
     7921 caacatcttt taatctcgta cctttgagat ctgcaccaac aacaatagcc attttgacca
     7981 cctcctaaat tttagaaaga aatgctacgt agaagtagaa aacattttgc aaaatgttcg
     8041 aaaaaacgtt ttcatc
//
LOCUS       DACEQH010000088         7660 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.091, whole genome shotgun sequence.
ACCESSION   DACEQH010000088 DACEQH010000000
VERSION     DACEQH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.091"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(365..1156)
                     /gene="msrA"
                     /locus_tag="IW833_002486"
     CDS             complement(365..1156)
                     /gene="msrA"
                     /locus_tag="IW833_002486"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1308548.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
     gene            complement(1347..1982)
                     /locus_tag="IW833_002487"
     CDS             complement(1347..1982)
                     /locus_tag="IW833_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1308549.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(2248..3300)
                     /locus_tag="IW833_002488"
     CDS             complement(2248..3300)
                     /locus_tag="IW833_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1308550.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(3313..4251)
                     /locus_tag="IW833_002489"
     CDS             complement(3313..4251)
                     /locus_tag="IW833_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1308551.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(4702..5352)
                     /locus_tag="IW833_002490"
     CDS             complement(4702..5352)
                     /locus_tag="IW833_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308552.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(5452..5961)
                     /locus_tag="IW833_002491"
     CDS             complement(5452..5961)
                     /locus_tag="IW833_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAQ1308553.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(6207..7526)
                     /locus_tag="IW833_002492"
     CDS             complement(6207..7526)
                     /locus_tag="IW833_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ1308554.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDKRAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT         2398 a         1567 c         1204 g         2491 t
ORIGIN      
        1 tagttattgt ttgcgctttt atacggaatt tagttcatca ttaatgtgga attcgattag
       61 gtgtctatta attatcaaga tgattctcta caaaattcca tagaaaaatc aagattctac
      121 aattcgaatt ctctttcaca tggaaataaa ggctagtaaa aaaacaggag ttgtgacaaa
      181 agacttgtca tacctcctgt ttccttgcga cttgaagctg aatgctttgc gccacaacct
      241 tttcctcggt gttctcaaag caactcctcg aaataattca taactgtcca aaaatatgaa
      301 aagctatttt tgtcagttgc tcattatttt gtcggagctg aacgctttgc gccacaacct
      361 tcctctatct atgtcgctga aaaaataaat gaagctgatg cgaatgcttg atctttttat
      421 atcttttagg ttgcttctga taaaattgtt gatgatgatt ttcagctggc caaaaagtct
      481 ttgcttggcg aatctctgta acgataggac ggctaaatcg gttagattga atcagttgtt
      541 ctcttgattt ttcagccaat tgcctttgct tttcattaac aacaaataga attgaacgat
      601 attgtttccc ccgatcttga aattgaccaa aggcatctgt agggtctgaa atttgccaat
      661 aaatctccaa cagctcttca taggttattt gacgtgtatc aaatatgatc tcaacagctt
      721 caacatgccc agtatatcca ccactgactt gatcgtaatt tggttttttg gtctctccac
      781 ctgtataacc agataacact gagattacac ctggttttgt ttcaaaaggc tcaaccatac
      841 accagaaaca gccaccggca aaaatcgccc gttcttggta taagggtgaa gttatcttat
      901 gtttggcata atcataagcc tttgcactcg ctgactcccc tgtgattctt aaataaaaat
      961 ccatcgcatc tggtgtcaaa ttatctcgta gtgctagcgg acgcaagttg acctctagtt
     1021 tttctatcac ctgctttatc gacatttttg ctaggtcttc ttttgcctct tcaaattgtg
     1081 ttctttccca ttctcttgta tttcgattta aaataatatt ataaaggtct atttccatct
     1141 gctccacatc taacatgaag tcaccctctt tcaagttttc ttcttattca ttcaaaaaat
     1201 gactacctga aatttagcac aacgcataac aagttaagac taacttgctt ttcgctctcc
     1261 tcatctctat tcttagtcga acacatccac acgaatggtt acccatagag aaattctttt
     1321 acttaatcag cttcacactg ctttttctac tttcgtagtt gaaaaaaact taaacaaatc
     1381 atcggcaaat agttaaagaa aaaacttaag cgttgaaaga agcccaattg cttaaagaag
     1441 gcaaactgac taatcgacgg aatccgtgct aatccgtaaa gacctgcaat tagtaaacta
     1501 ataactgtta aaacaagata aaaatggcta agcgtacctt gaccactttg acgataaagc
     1561 agaacaagta acaatgggaa aagtagaaag cctgcatagc ctagcccaga acctatatta
     1621 tggatccaag tcgatagatt ccaagagctt tcattcgtat tgatactgaa taacccagta
     1681 aaaatacaat cacctatccc atataaacca actgttgcaa cagcaagtat tgcaagtgtt
     1741 tttgatgttc caataaatag gtgatacaac gcaggcaacg aaagaacaaa aaaaagtcca
     1801 gatacaactg accagaccaa gaaagcacgt ctgactggac tatctacgtc accaaacaca
     1861 ctaattacgg tagtcatttg attcagtttg ggataaaaaa ggccaaggat atacggtgtc
     1921 gataaatcac taatcacacc taataataaa aaataaaaac catatttttg caaaaaaatc
     1981 attttcttct cctacttttt agtgattatt cgatacatcc aacaatcaaa ttttttctca
     2041 ttttaacaca tatttttatt ctagttgcct tttgaacaat caataacata aaggtgagtt
     2101 tcttctacat tttaaaaaat cgaatggcac tgaagagttc agttgtcagc atcaatctcc
     2161 tactcaaaaa ggcaaaaaaa cgtaagtcag atgttgttga catctcactt acgcatccat
     2221 tgattgaaaa tttcttagta aaatctacta gcctaaaaat ttgaagactt cttctcgatc
     2281 atacagttta aatactttct cgcataaaaa ttgagtaatt agtgccgcaa aaacagggat
     2341 gactaaccaa caaagaacaa caagaaagcc atttagccct gcatctaaag aagccaacgg
     2401 accaacaagt ccgaccacgc cgaaaccagc agaagcaggc gtacctgaaa tagaaaatag
     2461 agcgacagga atagctgtaa tcactgcact aactaaacaa ggaataagaa tcacaggttt
     2521 tctaaataga ttaggcatca tcatcttcat agcgcctaaa gcaatcgcaa ttgtcacacc
     2581 tgacttatta accttccaag agttcactac taaaacaaaa gtggttgcag ctacgcccat
     2641 cgcagccgca ccagcagaaa caccatctag ttggattgcc attccgattg ctactgttga
     2701 aataggagag ataatcaaaa aggcaaagga acaaccaatc aagatagaca ttaacagcgg
     2761 ttgtaaggta gtgaagttat taatcaatga tccaattgca gaagtaattg ctgttacaaa
     2821 agggaataaa taaaaaccaa gtaaacctgc acctacacct acaacaatcg gtgttaacac
     2881 aatcgacact gacccaaaac tatttccaat cagtaaaatc agcccgacag caatcgatgc
     2941 tgtcagcatc gtattaataa tatcgcctgt accagcacca acgtaaacac ccgtttcacc
     3001 caaatcaggt gtgaatttga ctacaccaga accaacaaaa gaagctccgg ctacaaccat
     3061 cattctcatc ggatcaaatc caaattgtaa ggcaatcaaa ccaccaatga tcaaaggtgt
     3121 tgctagttga aaaataactg ctaactgtgc gatcgttacc gcaaagggat acgcactaaa
     3181 atattttaaa atggcaccta atactgcatt aggaatcaat ccaataatga ttcctgttgc
     3241 tgtaccagcg aggactttat tcagaaaaat tttcggtgat attttttgtg aagtttccat
     3301 tcaatctctc ctttatttta cgttcaaact atcttctttt gcatggacaa gctcagtcaa
     3361 gaaatcacaa taaggcgtcg caacaccata tttcttccct tttctagaaa ttgcgccatt
     3421 gatataatcg atttctgtta atcgatggtt cttaatcaag tcttgataca ttgaaggata
     3481 atgtaaccca attgtttccg ggtcaaaaca agattcacaa tgtgcaatga cttctgggac
     3541 atctagttca atcttctcta ctgctgctac tttggcaaac tcattgacaa tcgtcgccac
     3601 cattttatga gcagtcgatg tttttcctag ctcagccata ttaacgtcta agattgtgca
     3661 tagtccgttc attgttccgt tcacacatgc tttgcgataa atagaatagt gaatattgtc
     3721 agaaaaatga gcatttaatc ctgattcaga caatttgtct gctaattttt ttgcagctgc
     3781 ttcttttcca tcacctaaat tttgtaattc tactgaacca cttccaaata atttgacctg
     3841 accaggacct tcaagacctg ccgtccacat ggtatttcca atatagatat tttccattgg
     3901 tacaaatttc tcaataatat cttcatgccc gataccattt aatagacata agacctctgt
     3961 atcttttttg attaatgatt ggatatcctg cagcattttt tccagctgca tcgctttggt
     4021 aaatagaata atcagatcaa cttgatcttc tttttctact tcggattgaa gaacgattgg
     4081 tagttttgct tctacctctt taccattaaa atttgcttgt aatccatgct ctttgatttg
     4141 ttggacatgt tctgcccagc catcgattaa taatacttcg ttcccacttt ggtgaagcat
     4201 taacccaaag cgacttccca ttgcgccagc gcctgcaatt gctattttca ttgattcttc
     4261 ctcctttttc tttctattat attcatttga gaatcatttc acaaccttgt tttgttattc
     4321 ttctcttatt tatcatgttt atatccacca ttcaagttat tttatcagca aatcaataaa
     4381 aactggaata gaatcccaat ttcatcgtaa gagaaactag actagcaata agaaaataga
     4441 aacattcata gaaaaaacct cactcctctg attataaaag agggggcatt aacctcgtgt
     4501 atactaaaac cttccattca ttcgatggat gattttcttt ttgtgaaaaa atattcattg
     4561 tagtacgatg ttgcgcaaca cagacggatg gtattgctta ttatttatta aaatcgaaca
     4621 atacacttat ttaaaaactt ctttcccaga ttttcttcca tttttacagt tataactagt
     4681 attatcatga aaaatagcct tttattgtct atgggaataa taatttaaaa ttggtacttt
     4741 tttattaatt agttcatcgc ccaataatct ctccagtttc ttagcatctc ctgactcaac
     4801 cggaattatc caagcccagt ccaaataatt ttttaaaatc tcatcatatt ttgcaaatat
     4861 ttcattgcca tattccgtcc ttaaaattat attttcagtg ccagacaagt gatttgtatt
     4921 gagttgataa aaaagatctc catctaaaaa aatatcatta gaagaagatt tccccacagt
     4981 aatgaccatt ccatcagctc tatgtgttac atacaaataa ttgtatgacc tcatttcacc
     5041 taatgaatca attatttctt gccctttagc tctaatatat tgatctctat tacctatctc
     5101 actcttattt aacaaaagtt ttttcatgtc aaatttaatc ggctgtttta tttttgtaaa
     5161 ttcgcttcta ctactaatcc aatccgagat taatttttca acatgtccca gagtaaactc
     5221 tgatttattc gctaaatact ttttttgttg tattaaagac atctttcccc gaagctcaaa
     5281 attgagccta catcgattct ctatctcttc aaaaagcatc ttatcttcat cattcttata
     5341 ttcatcattc ataaaaccaa tccagctcct cgattaagat aatattttaa tctcatacaa
     5401 aaactagctc tcatcaaaac aaccattgtt ttgatttctc atgaaatttc tttatacttt
     5461 tctccgatta taaattccta accccttgag aaaattacgc atatatttat ctccgcaagg
     5521 cttaaaattt cgttggcctt cttttctcaa aaatgccccg atttcgcctt ttgatggata
     5581 gagttctgcc tcagcaaaaa cttctaaaat atcatcagtg gtcatagaca aggcgatttt
     5641 taattttttt agcaacacat tgttgatata tttgatttcc ttgctttttt gcggcgtgtc
     5701 tactatcggc ttatcttttt gcggaccacg cgccaagaga atcaggccat ttaggaatgc
     5761 gtcaaaaact tggttgttga tggttttttc atacacatta ttttctgcat tatctttgtt
     5821 cttatcctgt actttggtca gtaccgcctg agcttcttct ttagtcagtg gtacaccgcc
     5881 tagctcaaaa gctttaatca aatccgtatc tttcaaatca atcgcataac gcaatcgcaa
     5941 taacctatca ttgttattca tttgttcctc cgaagtattt agtctttact ttcttacgta
     6001 cttcctgaat aattcatagc tttaattctt tggcacattt tattgaaata tgtatcacta
     6061 atatccctat caactgattt tgactaaaaa gttacttcaa acattcactg ttcaaaaata
     6121 ttgaggaata aattttgggt gtaaggcatt ttgagcatac ttctccctgg ataacttccc
     6181 agtaaaaaaa tcgttagatt gttggattag atccattgac tggctcgcct caggcgagca
     6241 aagaccctgt agaattcatt ctgatacaca tgataactag ataatttgag atagactcgt
     6301 ctacccgttt ggactaattt cccagcaact ttcagaaact tcaatcgaat agaatgaatg
     6361 gtcattccct tttggacttc ttcaaagcca atcgttctta agaagttgac taagttgtaa
     6421 gctatcaagc tgagcatcat tcggacatga ttctccaaaa agcgaggact gtctgttttg
     6481 tcaaagtaaa agccagcttt cgcttcttta atgaagttct ccattgtgcc acgtttggca
     6541 tagatagaaa agatggtatc aggagaaaca ttttctgata gattcgtcac gataaattca
     6601 tgtcgaaaga gtagttcgcc cgcttcacgt gttgagcgta tacacacgcg acgactttgt
     6661 gaccaggatt gtgcttgata agtggtcgag aagtactgaa cttctcgttc ttcccacttt
     6721 tgattatcgc cataaagtac tgatttctca gctatttgac ctagtctacg attattcttc
     6781 agtcgaataa cataatgact cttttttgat tcacacgaat catacacacc aggtgtagcg
     6841 aacccgctgt ctccacgaac caagatgtca gtgtttggta gagagtgatt atagtgctct
     6901 aataaaggtg tgagaaactc ctttacgcct tttgatgtat attgatttcc tgaacgtagt
     6961 tcagctttta agaagtcacc agtcaatccg tcaaaagcta ccaatggatg gtaaccatag
     7021 gtttggtagt gtgtattata attcgtttgt tcttggtgac caaatgtatc tgaatgggtc
     7081 gaatctaaat caatgattaa ttccgtatca ttacggatga ggcgtgcttt atcgataagt
     7141 tcttggttca aagcttgaag ttcatggata ttctcctcag atagtcgatc taaaaatcgg
     7201 gaaagtgaag attgagaagc gagttctttc ctatctaaaa cagctttaaa cacaggatct
     7261 tgtctcagga gattagctgc agaatcagct gagtaaccag caattaattg cataatgaac
     7321 tgctcaagta tggataagtt gtcatgagta aaatacgctc gtttgtcttc aatgtgaatg
     7381 tgttgcttag ccagttcaga aaaaccaaag atgttcatca gctctttcac taggacgaga
     7441 cccgaatcac tcgataattg accacctgta tgagaaatag tgatatttga attgaatttt
     7501 acttggtttt tgtgtaagct agtcatgaaa agaactcctt tcttttggtt ggtttagtca
     7561 ctttaaccat agcagaaagg ggttcttttt tcatcactta acgggtgaag atgaaaaagt
     7621 aattgtaagc tgccgtgagg cagtttcaca tggttttaat
//
LOCUS       DACEQH010000089         7651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.092, whole genome shotgun sequence.
ACCESSION   DACEQH010000089 DACEQH010000000
VERSION     DACEQH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.092"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1014
                     /locus_tag="IW833_002493"
     CDS             <1..1014
                     /locus_tag="IW833_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1308555.1"
                     /translation="ELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQGTFI
                     DYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLNDTW
                     LVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDP
                     FTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQLVS
                     AHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGDINA
                     QILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQ
                     ERE"
     gene            1025..3229
                     /gene="topB"
                     /locus_tag="IW833_002494"
     CDS             1025..3229
                     /gene="topB"
                     /locus_tag="IW833_002494"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1308556.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            3470..4063
                     /locus_tag="IW833_002495"
     CDS             3470..4063
                     /locus_tag="IW833_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAQ1308557.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            4076..4975
                     /locus_tag="IW833_002496"
     CDS             4076..4975
                     /locus_tag="IW833_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1308558.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            4978..5451
                     /gene="ssb"
                     /locus_tag="IW833_002497"
     CDS             4978..5451
                     /gene="ssb"
                     /locus_tag="IW833_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1308559.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            5817..6077
                     /locus_tag="IW833_002498"
     CDS             5817..6077
                     /locus_tag="IW833_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308560.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
     gene            6522..6728
                     /locus_tag="IW833_002499"
     CDS             6522..6728
                     /locus_tag="IW833_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ1308561.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            6728..6979
                     /locus_tag="IW833_002500"
     CDS             6728..6979
                     /locus_tag="IW833_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308562.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTTETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTELEN"
     gene            6995..7411
                     /locus_tag="IW833_002501"
     CDS             6995..7411
                     /locus_tag="IW833_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308563.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            7393..>7651
                     /locus_tag="IW833_002502"
     CDS             7393..>7651
                     /locus_tag="IW833_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308564.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVE"
BASE COUNT         2833 a         1309 c         1573 g         1936 t
ORIGIN      
        1 gaactaaagc caaaacatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
       61 gcggtcgcct ttttatggaa agatccacag acaaatgaga cagtaggggc gagttatcaa
      121 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
      181 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gttaaaattt
      241 tttgagagta gtattgattt acttagctat gctgcactaa atcgtgatca gttaaacgat
      301 acgtggttag tttcgatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
      361 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
      421 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agttgatcca
      481 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
      541 gaatacaaag tcatttatga agaagtcgca aaagaagaaa agatcacacc agaagctatt
      601 atggccatac acaagacaga aaacaatctg caattaacga accaattggt ttccgctcac
      661 aaagtaaatg cgtcttttgg tcagcagctt tctgttaacg attcaataga agctattaat
      721 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag cttgtgaacg cgtggatggt
      781 acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgctca gattcttttc
      841 tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga atttgtacct
      901 gaagtaaaaa aggattggaa tgatcaattg aagcatgaaa tctaccaaca agagatcaga
      961 aagcaaaaac gtgccatgtt gtttcaacaa ggtagacagc aagaaaggga gtgaaatcgt
     1021 catgatgaaa accgttattt tagcagaaaa gccgtcacaa gcgaaagctt atgccgatag
     1081 cttctctaaa gccacccgaa aagacgggta ttttgaaatc caagatagat tgtttcctgg
     1141 tgaaactgtg atcacgtatg gttttggcca tttagtcgag ttagattcgc ccgatatgta
     1201 cgatgaaaat tggaaacaat ggtcattaga gcatttgcct attttcccta cacattatca
     1261 ctatcatgtt cctaaagata agaaaaagca attcaaggtg gtgaaacagc aacttcaatc
     1321 ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga tagcttggac
     1381 gatcattcaa caagcaggcg ccgatcatgg aaagatattt aaaagacttt ggatcaactc
     1441 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagatgcag aagagaccta
     1501 cccaaaattt gaagaagcac aagcacgcca gatcgccgat tggttgatcg gcatgaacgg
     1561 tagcccgctg tatagtttac tcttgcagca aaaaggcata ccaggaagtt tttccttagg
     1621 cagagtccaa accccaacgc tttacatgat ttatcaatta caggaaaaga ttagaaactt
     1681 ccaaaaagaa ccatattttg aaggaaaagc ccaagtaatc gctcaaaatg gcgcatttga
     1741 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
     1801 agaaaagggg gttcaattgg gaaaacagcc aggtacgatc caccaagttg aaaccgagaa
     1861 aaaaagtgcg gcaagtccac gtcttttttc tctatccagc cttcaatcta aaatgaatca
     1921 gctcatgaaa gctagtgcca aagatacttt ggaagccatg caagggttgt atgaagggaa
     1981 atatctaagc tacccacgaa cagatacccc ttatattaca gaaggggagt atgcgtatct
     2041 gttggatcac ttagacgaat acaaacactt tttaaaggcc gaagccattc ctactcctat
     2101 tcatacaccg aatagtcgat atgtgaataa taagaaagtc caggaacatt acgcgatcat
     2161 tcctacaaag acggtcatga cggcagcggc ttttgaacaa ttaagtccac tgcagcaggc
     2221 tatttatgaa caagtgttaa aaacaacggt ggccatgttt gcggaaaagt atacctatga
     2281 agaaacaacg atcttgacgc aagttcagca gcttcagcta aagacagtcg gaaaagtacc
     2341 agtagatcta ggttggaaaa agctgtttgg caaagagagc gacagaaaag aaaaggaaga
     2401 agaaccgcta cttcctaaag tgacaaaagg agaagcggtg acggtagatc tacaagtcct
     2461 tgaaaaagaa acgaagccac cacaacctta taccgaaggt acgttgatca cagcgatgaa
     2521 aacagctgga aaaaccgtgg atagcgaaga agcacaatcc atattgaaag aagtagaagg
     2581 aatcggtaca gaagcaacac gagcaaatat tattgaaact ttgaaacaaa aggaatatat
     2641 caaggtagaa aaaaataagc ttgtggtgac aaataaaggg attttacttt gccaagccgt
     2701 ggaaaaagag ccgctgctaa caagtgccga aatgaccgcc aaatgggaaa gttacttatt
     2761 aaagattggt gagcagaaag gaacacaagc tactttctta gctaatatcc aaaaatttgt
     2821 ctctcacttg ttggaagtcg taccgggaca aatccagtca acagattttg gatcaacgtt
     2881 acaagaggtg aaagcagcca gtgaaaaaca ggaagcaacg cgtcatttag gtgtttgtcc
     2941 aaaatgtcgg gaacaagaag tcttgcttta tcaaaatgtg gcggcatgta cgtctgaagc
     3001 ctgtgatttt aaattatgga caacgattgc caaaaagaaa ctcacagcca cacaactgaa
     3061 agagatcatt caaaacggcc gaacaagcca atcagtcaga ggactaaaag gtcagaaagg
     3121 ttcgtttgaa gcaacgatcg ttttaaaaga agactttaca acaggctttg aattttctga
     3181 aaagaaaaaa acgaactata agaaaagaac acgccgcact acaaaataat gaaggggtta
     3241 tcctatgcga aatacaacac acaaacaaca agaaatcgaa aatcaaaaag caaaaattcg
     3301 tttagaacaa gagaaattac gtgcaaataa aagaagactg caacaattaa agaaccagca
     3361 gcgcattcaa aaagaaatcg aattagatcg atagacgtta atcttgttac aaataaacta
     3421 ctatgatata tttgatgtag tgtatttgta acgaagggag gggaagtaaa tgcaagataa
     3481 actgaaagaa ttgttggcta ttcgcgatgg aaatttatct atgaaggaag ccaggaaatt
     3541 tggaatcgca gcaaccactg tacaaagatt ggtgaatcaa ggaaaattga tcaaagttga
     3601 tcgtggttat tatgtagagg atggtcatgg aatagatgat ttattttggc ttcagcaacg
     3661 attttccagg ggaatcttct ctcatgaaac gactttagat atttaccagc tttctacgaa
     3721 tatgccgaaa ttcattcact taacttttcc ccatggctat aacgttgatc gttcaattac
     3781 aaaagagcag cagttacaat ttcatttcgt gaagaaagaa gtttatgaac taggaaaagt
     3841 agagggaact tcctttcaag gaaatcctat tactatgtat gataaagagc gtacactttg
     3901 tgatatttgg aatccgagat atgaatttga atatgaaatg aaattagaag cattaaaaga
     3961 gtacatggaa gacccattga gagatccaag taaattaaga gattacatga caaaactaca
     4021 tgtagaaaaa gaaatgaaat accacatgca aagcttatat tgatggagga aaaagatgac
     4081 accgacacaa tttgcagcaa aaacaagaaa tttagcaaag agtacaggat taaatgcaca
     4141 gttagtgcaa cggcatttta tgatggatcg tctaattgaa caaatttctg aatcaaagta
     4201 caaaaatgac ttcattttga aaggcggctt tttattagga tcaaaatatg gaatcgatag
     4261 aaggacaaca attgatatcg atacgacatt tagaaatagc aaattaactg aagaaaaatt
     4321 gaagagcatt tttaatgatt taactagaaa acccacaaaa gaaggcattg ttttctctat
     4381 tcaaggaatg aaggaaacaa gagaagccga tttttatcct ggatttcaaa ttaaattggt
     4441 ggctaattta gagaaagtac gtgtaccgtt taaaatggac gtgacaacag gagattctat
     4501 ttatcctgaa gtagatacac actatcacca attaatgttt gaagataaaa aagtggagat
     4561 tccagcttat ccaacagaac aaattattgc agaaaagcta agtgctacgt tcagctttgg
     4621 cacagataat tcgagagcaa aagattacta cgacttattt accattccga aactagaaaa
     4681 tatcgatacg aaagagttat acaaaagtgt tcgaaataca tttacaaaac gtggggatac
     4741 aaaaccgttg tccttttatt atgaaagaga aatggataag atcagaggaa atgactattt
     4801 ggaaaagcag tgggaaaaat atcgctcagt taacacgttt gcgaattcca tttcatttac
     4861 agatacagtc aacgaaattg atcacctaat gaaaagtgtg atcaaaattg aaaatcaaga
     4921 aagaaaacgg cagtttaaaa gatcacaaca aatgaatcaa gaaatggaga gataaagatg
     4981 attaataatg tgacattagt aggacgctta accaaagatc ccgatttacg ttatactgca
     5041 agtggcacag cagtagcgac ttttacccta gccgtcaacc gaaatttcgc gaatcaaaac
     5101 ggcaatcgag aagccgattt tattaactgc gtgatttggc gcaaaccagc agaaacaatg
     5161 gcgacattag ctaaaaaagg cggcttgatc ggtgttgtcg gacggattca aacacgaact
     5221 tatgacaacc agcaaggaca acgtgtgtat gtgactgaag ttgtagcaga taactttcaa
     5281 ctattagaaa gtaaagcagc gacagagagc cgtgcgcacg ctgatcaatc aaccaccagt
     5341 ccaagcccca ctacctttga acaacgggat acaaacaatg gattggacgc ttcacaaaat
     5401 ccatttggtg gacaatcaat cgatattagc gatgatgatc taccattcta agtagcattt
     5461 tctattggtt gcatttgcta cggtttatgt tataatttag tcaacaaaaa gaattttcaa
     5521 tctataacca aaaaccacta gctggcgggc tagtggtttt cttttacgtt tctattataa
     5581 gaattaacgg gtaggcttac tgtctttctt ggcaaattta caatcaagcc atttgcagat
     5641 atagtgcgcg gctacttctg ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc
     5701 tataacctcc tccccttgcc agattaggcc ggggtagcag acagcaagac tattatagca
     5761 taattgaaat attcaaatga ttggattttt gctataattt tagcgaggtg attgtgatga
     5821 acaaatatgt tggtttgctt gataaaatcc gagtaataaa aacgcaaccg ttattggttc
     5881 gctttacttt acaaacgatt catgaatcga ttaattgtgt agttgcagat attgaaatta
     5941 tcgataaatt gctaattatg gatgatggaa aatacaatat tgccgtcaca ggtcacttca
     6001 ataaacgaaa ccagctagtt attgctagca tgtatgttag aaatcccgat cagtttacgc
     6061 gattgatggg catataaaaa aagaagagtt gcaatcctaa tgtaggactg cagctctttt
     6121 tgttttacat agaaacaaga aaaatgaaaa ggccttttta ggaaggcgcg agcgccttct
     6181 caattgaaaa acctctctca ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac
     6241 gttgcacgtt cactaagata aagggtcttg caaagcaaga caaaatagaa aaaacttcct
     6301 ctcaccaaaa ggttcgttcc tgttcgttat ctctttattg atttgccaga ccggcaacaa
     6361 gttgcctttt atcaccaaaa agcgacaagg acaagggtaa atggcacgct gcgcgccctt
     6421 gtccttgtcg cttttcgttg attcattctg gccaacaaat caataaagaa agaactaacg
     6481 aaaggaagta aaacctttcc taagaggagg cagaatcaat catgcgaaag aaatttgatt
     6541 attggggtgt tcccttcagc gaattattcc caaactatca tgcaccacat actgtcgaat
     6601 gcgattgtgg agaaagagcg aaatgtatca aaagctatcg tttgtatcaa tgtccaacat
     6661 gcgggaaaaa atataccttg tcttatggcg attatatttt gattgatgag aaaggaaaga
     6721 aacgttgatg aaaacacaac acaaattatt gactgtaaaa gaagtcgttc aaattttggt
     6781 ggacaaagaa gtcatttctg aaaaagtagc agaagcttta cttattccgc ggtactggac
     6841 gacagaaacc cctttagtag aggatgcacc gactatgcta tttgaagacc tagtgaagat
     6901 tgcaggatat gaactgcatt atcaagaaaa cttggaaact tatgaaaacg aatattggtt
     6961 aaccgaatta gagaactaaa aaggagaaaa aaatatggct caaaattact atgacgaatt
     7021 tgtcaaatta cctttggata aaatggcaca aaaaatggag gatatgacat tcctatacaa
     7081 tgagacacgt gttccaaaaa aacattataa ggaaagattg tcggtagctg tggaagaaat
     7141 gattgaatca ggtgtcgaaa tgaatctcat tgcgacgtat tatcgaacac tggaagaact
     7201 gaaaaagcaa aatgcaaaat ggtttttcca agccttacta tgcttggaag taggcgtcaa
     7261 accaagcaca atcaaacctt ctgaatacca agctttagaa ctaacgtatg cgaaatttat
     7321 cgaaacgaaa aaagctaaga ctgtttcaag tgaatggtta gattattttg aaaatatcaa
     7381 taagtatggc gcatgttaca tcatgaagta atcagacgga ttcaaaaaga ctttagttgt
     7441 atggaagaaa tgaagtactt tgttcaaaat gttgaagaac agttatattt ggaaagcgaa
     7501 acgagtcaaa caaaggtcac agtcagccat aatcgtttag atgtgtataa acgtcgtcct
     7561 gaatcattaa aaaaaggagc ggagcttcta cgactagaaa cagaaaaata cgtcatgaat
     7621 gtttacctag aaaaaagtga gttggtcgaa a
//
LOCUS       DACEQH010000090         7598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.093, whole genome shotgun sequence.
ACCESSION   DACEQH010000090 DACEQH010000000
VERSION     DACEQH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.093"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(137..427)
                     /locus_tag="IW833_002503"
     CDS             complement(137..427)
                     /locus_tag="IW833_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308565.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="IW833_002504"
     CDS             complement(545..805)
                     /locus_tag="IW833_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308566.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="IW833_002505"
     CDS             complement(942..1355)
                     /locus_tag="IW833_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ1308567.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="IW833_002506"
     CDS             complement(1811..2296)
                     /locus_tag="IW833_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308568.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2680)
                     /locus_tag="IW833_002507"
     CDS             complement(2435..2680)
                     /locus_tag="IW833_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ1308569.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="IW833_002508"
     CDS             2873..3673
                     /locus_tag="IW833_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1308570.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="IW833_002509"
     CDS             3692..5560
                     /locus_tag="IW833_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1308571.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRSYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="IW833_002510"
     CDS             5553..6044
                     /locus_tag="IW833_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ1308572.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /gene="srlA"
                     /locus_tag="IW833_002511"
     CDS             6032..6586
                     /gene="srlA"
                     /locus_tag="IW833_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ1308573.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..>7598
                     /locus_tag="IW833_002512"
     CDS             6604..>7598
                     /locus_tag="IW833_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ1308574.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1214 c         1442 g         2235 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga agctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg ttcccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctg
//
LOCUS       DACEQH010000091         7402 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.095, whole genome shotgun sequence.
ACCESSION   DACEQH010000091 DACEQH010000000
VERSION     DACEQH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.095"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            68..151
                     /locus_tag="IW833_002513"
     CDS             68..151
                     /locus_tag="IW833_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1308575.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            276..1013
                     /gene="erm(B)"
                     /locus_tag="IW833_002514"
     CDS             276..1013
                     /gene="erm(B)"
                     /locus_tag="IW833_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1308576.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1183..1443
                     /locus_tag="IW833_002515"
                     /pseudo
     CDS             1183..1443
                     /locus_tag="IW833_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1606..2271
                     /locus_tag="IW833_002516"
                     /pseudo
     CDS             1606..2271
                     /locus_tag="IW833_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2307..2552
                     /locus_tag="IW833_002517"
     CDS             2307..2552
                     /locus_tag="IW833_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1308577.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2655..3524
                     /locus_tag="IW833_002518"
     CDS             2655..3524
                     /locus_tag="IW833_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1308578.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3505..4239
                     /locus_tag="IW833_002519"
     CDS             3505..4239
                     /locus_tag="IW833_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1308579.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4272..5184
                     /gene="ant(6)"
                     /locus_tag="IW833_002520"
                     /pseudo
     CDS             4272..5184
                     /gene="ant(6)"
                     /locus_tag="IW833_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5181..5723
                     /gene="sat4"
                     /locus_tag="IW833_002521"
     CDS             5181..5723
                     /gene="sat4"
                     /locus_tag="IW833_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1308580.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5816..6610
                     /locus_tag="IW833_002522"
                     /note="aph(3')-IIIa"
     CDS             5816..6610
                     /locus_tag="IW833_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1308581.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6718..6906)
                     /locus_tag="IW833_002523"
                     /pseudo
     CDS             complement(6718..6906)
                     /locus_tag="IW833_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6886..>7402
                     /locus_tag="IW833_002524"
     CDS             6886..>7402
                     /locus_tag="IW833_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1308582.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIE"
BASE COUNT         2593 a         1120 c         1556 g         2133 t
ORIGIN      
        1 tgtgtataat aggaattgaa gttaaattag atgctaaaaa tttgtaatta agaaggaggg
       61 attcgtcatg ttggtattcc aaatgcgtaa tgtagataaa acatctactg ttttgaaaca
      121 gactaaaaac agtgattacg cagataaata aatacgttag attaattcct accagtgact
      181 aatcttatga ctttttaaac agataactaa aattacaaac aaatcgttta acttctgtat
      241 ttatttacag atgtaataac ttcaggagtg attacatgaa caaaaatata aaatattctc
      301 aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag
      361 aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta
      421 aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag
      481 aaaaattaaa actgaacatt cgtgtcactt taattcacca agatattcta cagtttcaat
      541 tccctaacaa acagaggtat aaaattgttg ggagtattcc ttaccattta agcacacaaa
      601 ttattaaaaa agtggttttt gaaagccatg cgtctgacat ctatctgatt gttgaagaag
      661 gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag
      721 tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa
      781 acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc
      841 tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa
      901 atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc gttacttatg
      961 agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg
     1021 agtcgctttt ttaaatttgg aaagttacac gttactaaag ggaatggaga taaattatta
     1081 gatatactac tgacagcttc caagaagcta aagaggtccc tagcgcctac ggggaatttg
     1141 tatcgataag gggtacaaat tcccactaag cgctcgggac cccttgtagg aaaatgtcct
     1201 aagtgtggca acaatattgt attaaaaaaa tcgttttatg gttgttcaaa ttatcctgaa
     1261 tgtaagttta ctttagctga acattttaga aagaaaaaac taaccaaaac gaatgtaaaa
     1321 gaattactgg agggaaaaga aaccctggta aaaggaatca aaaacaaaga gaaaaagccc
     1381 tacaatgccg ttgtaaaaat tggggaaaag ggatatattg attttatctc tttttcaaaa
     1441 taaacatggg gctgacccaa aagtagctcc tatatgaaaa atagaggtct ttccatttag
     1501 atagtagtgg aaagacctct atttatcttt tgaaagtgaa tacaaaaata aagcacactc
     1561 tgctataata taggtgatta atccatttga gagaggtgct ttatcatgta tcaaaattat
     1621 accataaacc aactttgttt gcctatagat cttgaaatta aattagaaga aaatgatttt
     1681 gctcatgcca ttgtgcagtt tgttgactct attcccgatg aagtctttct tccttattat
     1741 caatctatgg gtagacccca atatcatcct cgtatgctac tttccattat tctttgtgcc
     1801 tatattcaag gggtttattc gggccgccaa atacaaaata tgctgataga tagtattcgg
     1861 atgcgctact tatctcaaga gcaattccct aactttcgta ctattaatcg ttttcgcgtt
     1921 catccgatta tgaacgatat tctagatcat tcatttgttc aatttcgtga gctactggtt
     1981 caaagtggct taatcagtgg cagtgctcta tacattgatg gtacaaaaat tgaagcggat
     2041 gcgaataaat actctttcgt ttggaaaaaa agtattttaa agtataagga tagccttgat
     2101 caaaaagccc ttgaaaacta ccaacaaatg gtggaaacaa aaatcttacc tgagttaata
     2161 gatgatttaa aggatgaact ttctatagaa gagattgaaa ggattcgtca atctttggaa
     2221 gaaaaggaaa atcaattaat tgaagcgatt gaccaaaccg aaacagtaga agggggctga
     2281 cccattatta ataaggagtg gacaacatga ttgatgtaaa tgagctattg gaagaagcta
     2341 ttagagaaac tgaaaatcta aatgatggcg aagtttttct tgttaaagat ttattcaagg
     2401 gatatgtggg gaataggata cccagaaagg atcgacttct gcttggaacg ttatttttaa
     2461 accatgttag caaaatgaat ggtaatttaa aggctattga aaagacttca tctaatcagc
     2521 agaggtataa aaagacaatt gataagtttt aactatcttt tgtgaaatta aaaaaattat
     2581 aaatatgttg agtagaagca aaagatttgg cttggaattt ttacagcagt gaatcgataa
     2641 aaggagcatg cttgatggta gataatatta ttaaatcagt agcagagaaa ttatcctctc
     2701 tgtcttatat agaaggtatt gttttaggtg gttcacgtgc aaggggcacc catacagagg
     2761 attcggatat agatatcggc atctattaca attcagaatc atttgacata aatactatta
     2821 atcaattcgc tacaaagctg gatgatgagc atagaaataa ccttgttgta cctcccggag
     2881 catggggtga ttggattaat ggcggcggat ggttagtcat aaacgggtat catgtggatt
     2941 taattttacg tgatattaaa cgtgtggaac aaataatgaa agatacagag cacggaattg
     3001 ttactgccaa ttatcagact gggcatcccc atggttatat tagtgcaatg tatcgaggag
     3061 aattagcgat tagcaaaata ctatatgcta agaatgaaag cttatgcgaa ttaaaaaaac
     3121 aggcagaaac ttatcccaat gctttgcaga aaagtttagt taactttttt atgtttgaag
     3181 cagggttctc tttaatgttt gtaaaagcaa attcgggaac agacgataaa tattatattg
     3241 cgggtcatgt ttttcgtata gtttcatgtt taaatcaagt gttatttgca tgtaataatg
     3301 cttattgtat caacgaaaag aaagctataa aactgcttga aacttttgaa cataaacctg
     3361 aaaaatatac cgagaaggta aatcatattt ttgaagtact cggtatctca ctttttgaat
     3421 gctacgacat gaccgagaag ctttataatg aagtgaatga aattgtatcg gagataaata
     3481 actttttaaa cgaggagagt tcagatgaaa gaaaacaaat atgatgataa tatatttttt
     3541 caaaaataca gtcaaatgag tcgctcgcag aaaggactgg ctggtgcggg agaatgggag
     3601 actttgaaaa agatgctacc tgattttaag ggtaagcgtg tgcttgattt aggatgcggc
     3661 tatggatggc actgtatata tgcgatggaa aacggtgctt cctctgtagt aggtgttgat
     3721 atttctcata aaatgctcga agtagcaaaa ggaaaaaccc attttccaca gattgaatat
     3781 gaatgctgtg ccatagaaga tgtggatttc ccagaggaga gctttgatgt aatactaagt
     3841 tcgcttgcgt ttcattatgt agcagactat gagaatttaa taaaaaagat atataggatg
     3901 ctgaaggctg gtggcaattt agtttttaca gttgaacatc ctgtttttac tgctcatgga
     3961 acacaagact ggtattataa cgaaaaagga gaaatactgc atttcccggt ggacaattat
     4021 tattatgagg gcaaacggac agctatgttt ttggaagaaa aggttacaaa atatcataga
     4081 acactgacca catatctaaa tacactgctt tcaaatagtt ttataataaa tcagattgtg
     4141 gagccacagc cgccagagaa catgatggat attccgggga tggcggatga aatgcgacgc
     4201 ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat aatatagaaa aaataaacga
     4261 ggagtatgta aatgagatca gaaaaagaaa tgatggattt agtactttct ttagcagaac
     4321 aggatgaacg aacgtattcg aattgtgacc cttgaggggt cacgcgcaaa tattaatata
     4381 cctaaagatg aatttcagga ttatgatatt acatattttg taagtgatat agaaccgttt
     4441 atatctaatg atgactggct taatcaattt gggaatataa taatgatgca aaagccggag
     4501 gatatggaat tattcccacc tgaagaaaag ggattttcct atcttatgct atttgatgat
     4561 tacaataaaa ttgatcttac cttattgccc ttggaagagt tagataatta cctaaagggc
     4621 gataaattaa taaaggttct aattgataaa gattgtagaa ttaaaaggga catagttccg
     4681 actgatatag attatcatgt aagaaagcca agcgcaaggg agtatgatga ttgctgcaat
     4741 gaattttgga atgtaacacc ttatgttatt aaaggattgt gccgtaagga aattttattt
     4801 gctattgatc attttaatca gattgttcgc catgagctgc tgagaatgat atcatggaag
     4861 gtcggcatcg aaacaggctt taaattaagt gtaggcaaga actataagtt tattgaaagg
     4921 tatatatccg aggatttgtg ggagaaactt ttgtccacct accggatgga ttcctatgaa
     4981 aacatatggg aagcattatt tctatgccat caattgttca gggcggtatc cggtgaggtg
     5041 gcggaaaggc ttcattatgc ctatccggag tatgatagga atataacaaa atataccagg
     5101 gacatgtata aaaaatacac tggtaaaacc ggctgcctgg atagcacata tgccgctgat
     5161 atagaagaga ggcgggaaca gtgattacag aaatgaaagc agggcacctg aaagatatcg
     5221 ataaacccag cgaaccattt gaggtgatag gtaagattat accgaggtat gaaaacgaga
     5281 attggacctt tacagaatta ctctatgaag cgccatattt aaaaagctac caagacgaag
     5341 aggatgaaga ggatgaggag gcagattgcc ttgaatatat tgacaatact gataagataa
     5401 tatatcttta ctaccaagac gataaatgcg tcggaaaagt taaactgcga aaaaattgga
     5461 accggtacgc ttatatagaa gatatcgccg tatgtaagga tttcaggggg caaggcatag
     5521 gcagcgcgct tatcaatata tctatagaat gggcaaagca taaaaacttg catggactaa
     5581 tgcttgaaac ccaggacaat aaccttatag cttgtaaatt ctatcataat tgtggtttca
     5641 aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg
     5701 ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat
     5761 aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc
     5821 taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga
     5881 tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata
     5941 tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga
     6001 catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca
     6061 tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta
     6121 tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt
     6181 tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga
     6241 attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga
     6301 cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga
     6361 ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa
     6421 agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc
     6481 cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt
     6541 tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga
     6601 attgttttag tacctagatt tagatgtcta aaaagcttta actacaagct ttttagacat
     6661 ctaatctttt ctgaagtaca tccgcaactg tccatactct gatgttttat atcttttcta
     6721 aaagttcgct agatagagtt ctatattaga gatgtaaaga gttttggtga agaggtttgt
     6781 gagtatggct tttataacca gggtgcaatg agaagcacta acagcagcta gcttaagaac
     6841 gcataggata tactttttat ggaagtcaca gaggaggaat aaaatttgaa caggattaac
     6901 agagtaacct ctattcttat tcagctgcaa tcaaaaaaaa taattcctgc caaagaaatt
     6961 gcacagcgat ttaatataag cttaaggaca gtttacagag atatccggac acttgaagaa
     7021 gccggaatac caattggatc tgaggccgga aaaggatatt ttcttgtaga gggcttccta
     7081 cttccgccgg taatgtttac agcggcggaa gtgggtgcat tgattacagc agggaaattt
     7141 ttaaattgcc atggagatga atcatttata aaggattttg attcagctat gtataaaatc
     7201 aaatctattt tgaagcatgg tgaaaaaaac tatgcacagg agttggagaa cagtattaac
     7261 gtgtacagca catctggaca gaaaaataca ttggcggata acgttattgc agcaattcag
     7321 actgcaatct gcaataaaag ggtaatatca attcaatatc ctgcatcagg agggcaagaa
     7381 ccggaaagca gaatgataga gc
//
LOCUS       DACEQH010000092         7212 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.096, whole genome shotgun sequence.
ACCESSION   DACEQH010000092 DACEQH010000000
VERSION     DACEQH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.096"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..678
                     /locus_tag="IW833_002525"
     CDS             <1..678
                     /locus_tag="IW833_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ1308583.1"
                     /translation="IKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVNSAENAWTN
                     LKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIK
                     NWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNG
                     ISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAV
                     GGTLYAT"
     gene            668..1408
                     /locus_tag="IW833_002526"
     CDS             668..1408
                     /locus_tag="IW833_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1308584.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1405..4167
                     /locus_tag="IW833_002527"
     CDS             1405..4167
                     /locus_tag="IW833_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ1308585.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            4180..5088
                     /locus_tag="IW833_002528"
     CDS             4180..5088
                     /locus_tag="IW833_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ1308586.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            5088..5705
                     /locus_tag="IW833_002529"
     CDS             5088..5705
                     /locus_tag="IW833_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308587.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAESYTNERIPNFTFEKIGEV"
     gene            5709..6158
                     /locus_tag="IW833_002530"
     CDS             5709..6158
                     /locus_tag="IW833_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308588.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLTNITFEKVGTV"
     gene            6158..6682
                     /locus_tag="IW833_002531"
     CDS             6158..6682
                     /locus_tag="IW833_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308589.1"
                     /translation="MADIVQLKEDGVAKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCGTWP
                     EGSETRWILKIPDGFKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITQDPWPA"
     gene            6710..7117
                     /locus_tag="IW833_002532"
     CDS             6710..7117
                     /locus_tag="IW833_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308590.1"
                     /translation="MKTIYKVLYPMGYEEHQVEDNFPTSLPFVEVPPILFEKKEDETD
                     EGFGRRQQSQFFNFTENKWEEAVTQDYSKKLELLENLSIGLQVDNAALKKSNEELTEK
                     ADSMAQLNAKLMLNDLTINKKIEAIEKQIGGAS"
     gene            7117..>7212
                     /locus_tag="IW833_002533"
     CDS             7117..>7212
                     /locus_tag="IW833_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1308591.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRQFVPLCITDE"
BASE COUNT         2600 a          985 c         1522 g         2105 t
ORIGIN      
        1 attaaagata cggcatacaa tacgtggatt tctataaaaa ataccatgat taatacatgg
       61 aataacatta aggactcttt ctggaatatt gttactggaa ttgttaattc tgctgaaaat
      121 gcatggacaa atctaaaaaa tggtgtttca aatgctatta atcgggtaaa agaaatcttt
      181 aattcgttaa gggaagtcaa tttatttgaa attggcaaga acatcattga tggacttata
      241 aatggtgtaa aagaaaagtg gaatgctttg aaagatacta tcaaaggtat tgcaggtaat
      301 atcaaaaatt ggattaaagg agctttagat attcattctc catcaagatg gatgcgtgat
      361 atggttggta aaaatattgt tcaaggtatt atcgttggta ttgataaaga gcaaagtaag
      421 ttagatcaaa ccatgacaaa tttagtaaaa acaccgtctg ttcaacctgt agtaaatgga
      481 ataagtcacc taccaattac acaaacaaag caaaatatat ctgctagtga aacaaaagaa
      541 attcatttac atttaaatgt ttatggagat ttacctgatt caatggttcg gaagattacc
      601 acaaaaatta aaacagaatt gacaagacaa atgaaacgag atgctggtgc agtaggagga
      661 acattatatg caacttaaga gaggacaatt ttttattaat caacattatt cttctgaatt
      721 taatgtgtat attcaaaaca ggcctgcttc tgtttcagct agtcgagtaa ttgaattgag
      781 agagcgagaa ggaaatgatt ctatcattat tgataaaggc tattacagaa atgtgactag
      841 aaagattgag tgttactata aagcaccttc aattgatgtg gtgcaggagt gggaagatcg
      901 aatcactgaa tggttagata tgtcgtctta tagtgatttt attctttact atgatgaaca
      961 atatatttat caagctgtag taatagaagg acctgaattt aaaggaacaa gaaaaacagg
     1021 aaatattgtc ccttttgaat ttacagtgag cattcgacca tttaaggaaa attacaatgg
     1081 tagatttact attcgacaaa cagaaacttt tgaaatttat aacccagaga agtattcttc
     1141 aaaaccgctt attaaattga gtggttctgg agatgcttct ttttatatta ataaggacaa
     1201 atatgatttg aaatcgttgg acagagaatt atttatagat tctaaattag aagaggctta
     1261 ccgaaaatta gatggtaatt tagaacatca ggatcaagtc actttatttt tagattttcc
     1321 atttttatat cctggaaaaa atgaaattaa atggacgaaa aatattcatt catttgaaat
     1381 aatgcctagg tggtggagaa aagtatgaaa ccaagaatat atagtcctac tgaaacagat
     1441 ttctcaacga atggcttagg aattttaaag gattgtacaa gatgtgaaat atatgaagta
     1501 gctaatggaa aatatgaatt agagttggat tatcctttag gaactagatt tgatgaatat
     1561 tttgaaaatg actatcaaat aaaagcaaag ccaaacgatc aagaagagta tcatatcttt
     1621 tttattgatg ataaagatat agatactttt ttaaatacag taactattta tgctcagagt
     1681 cgtacaaatc gacttggaag acgggtagtc actcttgcgg aagtagactc taaaactggt
     1741 caagaagcga tatcaattat tgaaaccaaa atggataaaa aatctgacat acgactttat
     1801 tctgatatta cggccgtttc tagcacaatc tttgaagcga gaaatgtttt aaattgtatt
     1861 gctggtgaac aaggatcatt gcttcaatat tggggtggag aaattaaacg tgaaccattt
     1921 aagctttctt tgttaaagcg aagaggtcgc aataatgttg gaacgattcg gtatggaaaa
     1981 gatatgtctg gtttaaaggt caaattagat tggacaggta taaaaacaag acttattcca
     2041 tatgctgatc ctcaaagtga ggtaggtacg actaatcgaa tttatggttc gccagtagat
     2101 agtgcctata ttaataacta tcctgatgtg tatacagagc atgttcagtt tacagaagaa
     2161 caaggagtaa aagacattaa gagcttaaat aaaatagcta aaaattactt taaaacgatc
     2221 aatcctggct gtgataagcc taaagtttct attacggttg aatttgataa gttgactgat
     2281 agtgaagaag cgaaagaatt tgcgaagatt agaaattatg gtctgtttga tacatttaaa
     2341 atatatcata aaaaatataa tatttatctt gaatcaaagg ttagtggact acaatacgat
     2401 tctttatctg aaaaaacttt gaaattagaa gcaggagaca ttcaagttgc tttttatcag
     2461 caacaagctg taactattca ggataaatta aaagattatg caacgaataa ttatatgagt
     2521 gattttaatg attatgtttc ttcaatgatt acaggtcaag gcagtgcagg aggatatgta
     2581 agtttatggc caaaagaaaa accttccaac atctttatta tggataatcc agatttagaa
     2641 aaggcaagag aagtactaag aatgaataaa aatgggattg ctttttctaa gaaaggatgg
     2701 cgggggcctt ttacttcagc ttggacatta gatagtatat ttaatgccaa ttttatacaa
     2761 actggattaa tcaaagcaga tatctttcaa aattctttta acaagactgg agatgtattg
     2821 aaattagtta atggactact ccaaatttgg aacaacaaaa agaaaataat ggagttaact
     2881 aaaaaaggaa tggaattttg gaattctaag ggttcaattg gaacgattgg aacaactgat
     2941 tctgctggta atccttttcc tggggcttct actccaaccc ctattgaaga taattcttta
     3001 gttattcgta caaatggaga cggcaaatat attttgattt ctcctaaagt aggaaaagga
     3061 ttagttttat taggaaatgg taaagcaatt tattttggag acttagatgt acaaggaaaa
     3121 cttacagtta aaggaaaaga aataattcct ggacaaggtg gtggtccatc gggaggtgga
     3181 gaatctactg gtgggtatcc tagtgaatta aaaacagatg cagaaaagag agcttggaga
     3241 atttacgata ttttgtgtaa taacggattt acgaaacaat ctgcatgtgg aatattgggg
     3301 aacattcaac aagaaacagg aggaactttt gatcctgata ctgttcaaat aggtggacca
     3361 gcatacggat tagttcagtg ggatggttct tcatatcctc ttgttggtcc agcaacatgg
     3421 gatggaaaag tttatgttca aaacttattt aatgctgcag gtattaaaga accaataacg
     3481 agcttagatg cacaagttcg attgcttatt tggacattta caaatggaca atggatgggc
     3541 gtagtacaac ctacgacggt tgatggattt aaggcctgta ctgatccaag acaagcagca
     3601 tatgcttttg aacgaaacta tgaacgtccg gcagcgacac atcctgaacg tcaagattat
     3661 gcagttaact ggtataacaa atttaaagat ttaaaaccag gaggagctac tggagaagca
     3721 ggactaaaac atttagagtc tttgattgga caacgaattg gcaatggtca gtgttatggc
     3781 ttgtctgcag aatattcagg atatctgggt ggttgtggca tgggtgctgg aacaaaatat
     3841 ggtttaactc atgtgatagg aaatacttct gcagcatctg atattggtat tgcctatgat
     3901 tggtctgctg ttggttggaa agtgattcaa aaccctagat atgatcaatt agtagttggt
     3961 gcaattatta attgggcaag aggtggacaa gtgggttcat ggtttgcaga cggaacttat
     4021 ggacatactg gcgttatccg aggcttagct aatggtcgta tgcaaactta tgaacaaaac
     4081 acagagttag gtatgatttg tggaaagtta gatcgccagt attatagtgc aagtgcaatt
     4141 tcttccattg tcataccacc aaaataggga ggtgatttaa tggcaaagtg gaatgtcata
     4201 ttaagtacaa cagaacccta taattacgtg gggatgattc aagttcgaca aggcaataaa
     4261 aacactgaag ttatggaagc tacaattgtc gaaaatggtc ttccatatga tttatcagaa
     4321 tgtaaggtat attttgaatc agttgttggc ggaaaatatc cagtccaatt agaaacaaga
     4381 attgtggatg ctaaaaaagg gaaaatcaaa tatatttttg ataaatattc catgcagtgt
     4441 ttgcatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
     4501 caagacttct cctattttgt catcaatgct gtttcaaaaa cagaaggaga aatgggttct
     4561 tattggcaat caattgaaga tttaattgct gatatgacag attttattaa tgaaaataaa
     4621 ggcgatttta cggactggat gaatgaaagg aaagaagaat ttgatcgctg gagagaagaa
     4681 caggagaata gtttccaaga ttggagagaa ggacaagaat cagattattt gtcatggttt
     4741 gaatcaatca aggatatttt gaagtctatc gatccaggtg gagtaatgtt agccgaatta
     4801 atggatgcac gtgttgatat tcaaggagtt cgtcatgcgt ctatttcaga gcgcttgctg
     4861 gcagatatgg attatctata tcagaaatta cgagcaacac ttttcacgat tgaatacggt
     4921 gagattgaag tgactgatat tttgcaggat gatctctttt cagataatca cgaagtcgaa
     4981 aaagttggaa ctgtagaatt tccaattgaa gaaggagctt tgattattgc aactgttgat
     5041 gatccaaaac agaatgtttt cactcttgag aaagttgggg tgatttgatg gttaaagtaa
     5101 agagaatgat ggagactgat gaatctggtg ttaaacgtca atttatgcca attacacatg
     5161 tttctgcagt tttaggctta gaaaaaatga tatcaggaca atcaaaagtt ctttcggtta
     5221 atggtaaata tggtgcagtc attttaacga aagctgattt aggattagag aatgcaatta
     5281 ctgaactacc ttatgcaagc gagacaagcg atggtattct tactgctgaa atgtttcaaa
     5341 agatcgtaaa tggagaaggt ggaacatata ttttgccgat tgctactcct gaacaattag
     5401 gaggtataaa agttggggaa ctcttagaag ttactgaaga aggtgtttta tctgcaacta
     5461 agcaaacaga cttcaatttc tctgaagaat taaagggaaa attagaatct ctaaaaatat
     5521 taaaagcagg tgcaaatata tcaattgcag aagatggaac aattagttct acaggtggta
     5581 gtgggactgg tggtgttaat cagtcatatg tagatcaaaa attccaagaa gctgtaaatc
     5641 aagcagaaag ttacacaaac gaaagaattc caaattttac ttttgaaaaa atcggggagg
     5701 tatagaaaat gacagatatc gtaaaaataa aacaaagcgg tgtacaggtt tatccacaaa
     5761 ctcattggaa tgctatagaa ggcaaaccaa caacggtgaa gggagataaa ggcgatcctg
     5821 gccaagcagc aacaattacc ataggaaccg tttctagtgg ttcaaccgct tctgttacga
     5881 atgtaggaac atcatctgca gctagattta attttgtttt accaaaggga gataaaggtg
     5941 atcctggaat aaatgccaca actacagctg tagctacaac tacagcaaat gggttgatgt
     6001 cgtccacaga taaaactaag ttggatggaa ttgcagctgg agcacagaaa aatccaggta
     6061 atgctacaac tacgacagca ggtttaatgt cagcaaccga taaagtgaaa ctcgatggat
     6121 taaccaatat tacatttgag aaagtgggga cggtttaatg gctgatattg tgcaattaaa
     6181 agaagacgga gttgctaagt atttgaaaac tcatgccaaa ggaattgatg gtgtggaggg
     6241 tgttttagta aaagccacag gtaatgaaac ggtcttagga acgaaaaatt ttaaagacgg
     6301 attgcaattc aatggattgc ctgtgcaagc aggtatgatc gagcgagcta tcacactagc
     6361 tgatcgaagt gatacaacaa acgtaactga tgtgaatgga aaaataatca gaatcggaaa
     6421 catcgtattt cttactttta attttaaatg tggcacatgg cccgaaggat cagaaactcg
     6481 ttggatttta aaaatcccgg acggtttcaa acgtgatcaa ggctatccag cgcaaaccgc
     6541 gctttcacta gttagaaatg caagtcagcc agcggatgca cgtgcattca ttgatcaaag
     6601 tagcatcata caagccaaat caggttcagg tagttcatat atttcaggta tgtggatcac
     6661 tcaagatcct tggccggctt aacagattac taattggagg aagagtaaaa tgaaaacaat
     6721 ttataaagta ttgtatccaa tgggatatga agaacatcaa gtagaagata actttccaac
     6781 atctttacca tttgtcgaag ttccacctat tttgtttgaa aagaaggaag atgaaacgga
     6841 tgaaggtttc ggaagaagac aacagtcgca attctttaac ttcactgaaa ataagtggga
     6901 agaagcagtt acacaagatt attcaaaaaa attagaacta ctagaaaatt tatctatagg
     6961 tttacaggta gataatgctg cattgaaaaa atcaaatgaa gagttaacgg aaaaagcaga
     7021 ttcgatggcg cagttgaacg ccaaactgat gcttaatgat ttaacaatta ataaaaaaat
     7081 tgaagcaata gaaaaacaaa ttggaggtgc ttcataatgt ttagttttag cgatgtgaaa
     7141 atgatgtatg attggggctg ttttactgac gatcaagttc gacaattcgt tccactatgc
     7201 attacagacg aa
//
LOCUS       DACEQH010000093         7187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.097, whole genome shotgun sequence.
ACCESSION   DACEQH010000093 DACEQH010000000
VERSION     DACEQH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.097"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IW833_002534"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IW833_002534"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ1308592.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IW833_002535"
     CDS             complement(1172..1867)
                     /locus_tag="IW833_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ1308593.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IW833_002536"
     CDS             complement(1880..2596)
                     /locus_tag="IW833_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ1308594.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSILYK"
     gene            2926..3534
                     /locus_tag="IW833_002537"
     CDS             2926..3534
                     /locus_tag="IW833_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ1308595.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IW833_002538"
     CDS             3859..4182
                     /locus_tag="IW833_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1308596.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IW833_002539"
     CDS             4364..5734
                     /locus_tag="IW833_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ1308597.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            6081..>7187
                     /locus_tag="IW833_002540"
     CDS             6081..>7187
                     /locus_tag="IW833_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1308598.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKK"
BASE COUNT         2110 a         1445 c         1343 g         2289 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcgttatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttatata aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaacat actaagatta gaggggaaca tcattgacga atgatgttct cctctatttt
     5881 ctatttttag attatagtga agagtgaaag aaagttcgaa caaagccttg aggactacga
     5941 gtccctatca aaaaccgaac ttctttactg tcattttaga atcaggttta cgtatgttgg
     6001 ggccatgagc ccgtgtatag gaaattggaa tcgaaatgaa gcagtaaagt tctttcaaaa
     6061 cttaaaaagg aggaaaacaa atgccttaca ttcttgcatt agatgtttca atgggacaca
     6121 gctacagtgt cctatacaaa gacgacacct gcttatttga agacgagatt gaacacactc
     6181 aaagaggatt tcatgcatta ctcgaagaga tccagcactt gcctgagcgt ccattgattg
     6241 tttttgaagc aacggggatt tattccagac cagtcgaaaa gttctttcaa gatcatcact
     6301 tttcttactg tttgttaaac ccgttagagg cgaaaaaaca gatggaagaa agcaccttac
     6361 gcagttggaa aacagataaa tcggatgccc accggctcgc acagactcat cttaaaaatc
     6421 aacgtcagcc aaaagaagta caaagcaatt tttacttaga gatgcgcgat ttagcccgtt
     6481 tttatcaaga aattgagaag aaaatcactc gtctgcgcat ggatttacac aactgccttc
     6541 aattgacttt tccagagctt gaacagttct tttcaaacag gctgacccct tacgcattga
     6601 cattgatccg tctttttccc catccagatt ttgtattggc atctactcgg acaaaaataa
     6661 aaaataaact gatcaacgaa acacggaaaa aaatctccgc gaatcgtgcc gagcaaaagg
     6721 cagatcaaat catccactat gcacagtgtg cctatccagc tgttgaaaaa gacagtatcc
     6781 actgtcaaaa aacgatctat tacgccgaac ttctccaaga tttactcgaa caaaaaaaag
     6841 cactggcgac acaaatgatc aaaaaagccg aaggctctcc ttgctttttg ttgtaccaaa
     6901 cgttccctgg gattggcgca ctcacagccg ctttattgct cggtgaatta ggagatatca
     6961 cacgatttaa aacccataaa caattgaatg cctttgtcgg aatcgacatc cgacggtatc
     7021 attcaggtaa gtatactggc caagaccgca tcaataagcg tggtaatccc aaagcacgga
     7081 aaattatttt cttcaccatt cgaaatatga ttcgccagca acgcgcagct cctaatcata
     7141 ttgtcgatta ttactacaaa ttaaaaaagc aacctatccc taagaaa
//
LOCUS       DACEQH010000094         7018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.098, whole genome shotgun sequence.
ACCESSION   DACEQH010000094 DACEQH010000000
VERSION     DACEQH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.098"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            685..879
                     /locus_tag="IW833_002541"
     CDS             685..879
                     /locus_tag="IW833_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308599.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            1070..1319
                     /locus_tag="IW833_002542"
                     /pseudo
     CDS             1070..1319
                     /locus_tag="IW833_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1563..2393)
                     /locus_tag="IW833_002543"
     CDS             complement(1563..2393)
                     /locus_tag="IW833_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ1308600.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2601..3935
                     /locus_tag="IW833_002544"
     CDS             2601..3935
                     /locus_tag="IW833_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1308601.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3976..4887
                     /locus_tag="IW833_002545"
     CDS             3976..4887
                     /locus_tag="IW833_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1308602.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4884..5738
                     /locus_tag="IW833_002546"
     CDS             4884..5738
                     /locus_tag="IW833_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1308603.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIILLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            5735..6352
                     /locus_tag="IW833_002547"
     CDS             5735..6352
                     /locus_tag="IW833_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1308604.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            6354..6872
                     /locus_tag="IW833_002548"
     CDS             6354..6872
                     /locus_tag="IW833_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1308605.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         2108 a         1205 c         1399 g         2306 t
ORIGIN      
        1 aaaaattcta aaggattcct aaccatctta ttagctttta tagggtatat gttggttggg
       61 ctgctaaaat catacagtaa tgagttgctg aatttttcaa catttattaa tgatacatta
      121 gttccaagtt tgtttttcat tgtatttttt gctgttggtt attttataat aaagatatga
      181 tgactaattt agctcaacta ttccagcgtc agctgaagat attgcaattc ccgattcctt
      241 aatcatttat ctggatgaga cagaaagtga agttgcaaca gatgtgcatg tagttgagga
      301 taaaaatgat agtttaaaaa ttaatagttc agatacttta ctaagagtaa gtcctatctt
      361 ttcagctgtt atttagatgc aagtagcaac taagttaggt agtactcctg caatggatag
      421 gtatttgtgt gttcgatata taagtggtcg aagtatcaag gttatgtata ctggatggga
      481 gatagaagag ttaaaaattg gaggtcaccg ccacaaatgg aatttagatt tagaggtacc
      541 ttagatttgg tatagttatt aaacacaaga tggcctggtt tgtagggaaa gtcgaagagt
      601 atttttccac ttctgcgccc ctttaatttt tctagtaatg ccgtgaattt gtgattaatt
      661 ggaaatatga agaaaggaga gtctatgaaa acaacactag ttctattcta taaaaagcat
      721 ccttatttta cacttctgat aaatatattg ttggctagtg ttatcgggat ttctgttgaa
      781 tatctaataa ataaagattt cataggttct tgtttttata cggctttatt tcttggactt
      841 cttgaagcat tttccattta taaaaaatca aaaaaataaa atagattgcc gtaagagatc
      901 tttttcattt catttttttc tgaaaacccg ttgtttctgt cagtgaagca gcgggttttt
      961 agatttattg tctttagacc taattgagcg cgccgagcga ttgaaataca aaaccacttg
     1021 ctatgcgggt ggagcaataa agggaaatag tttagcccgt acaaagtaga tgtgtaaata
     1081 ccacattgta ttcacaccaa agtatagaag aaaaataatt tataatcaat atcgagccaa
     1141 tattcaggag atcatcaaga gattgtgcaa atctaaaata taaatttggg tatcgacact
     1201 tttgggtgga aggatactat gtgacaaaag taggacaaaa tgaagcgacc atcaaaaagt
     1261 acattcaaga acaagaaaaa catgatagtg cgttagataa gctgagtgta cgagagtaag
     1321 acgatccttt taagggctaa ggagtagtac ataaaccttt taagaggtgg gcaaaactgg
     1381 caaaggcaat atggcttgaa cataaaatat gaaagccagc gtctttaggc gctggccggt
     1441 gatttgagct tatagctcta gagaaaacca cccttttgaa gggtggttat gattaatgac
     1501 tttagtcagt tttgctcaca aagcgtgtgg aacaaaaaac gggtgtttct acgggtagat
     1561 atttatttct tgaatggagg tgtcccatac atttttggtg ttactggttc taattttggt
     1621 gcagcgcctt cgatcttagg ttctttcaac cattgatatt cgccttcgcc atccagtgct
     1681 ttgccactag cccaagaacc ttcactactt tggtctccct ctgagaagtt atagaagtca
     1741 taagcaatgt cttttcgttc tttgtcacgc gggaaagtac ttgggactaa gattgaacct
     1801 tctttttctt ctaattcttt cactgcacat tcccatagat tttgatgata gctatctctt
     1861 gcaaggagaa cagacagcat atcttttacg ccacgatcat cgatcatttc gtaaatacgt
     1921 gctacttgaa gtcttccttg tgattcggca tttaaattag ctctaaagtc tgctaacaag
     1981 ttgccactcg caatgatata ggcaccgctc catggattgc ccacgctgtc agcaggacga
     2041 ggtcctaagc cagctacgat cgcgtgctgt ggattcattc cagagataat ggcaccgatt
     2101 gttggatctt tttgcatcgc tgcttcttga tcttcaacag gcgcaccctc taataaacga
     2161 gcaaccattg tcgccaacat ttccacgtgg ccgatttctt ctgttgcaat gtccatcagc
     2221 atatctttat atttttcttc tcctcggcag ctccagcctt ggaacaggta ctgcatagct
     2281 acactcatct cgccatattg gccgccgatc agctcctgca gatatttcgc gattaatgga
     2341 ttcggacgat ctggttttgc ttcaaactgc aattctttct ggtgtctaaa catcaaaatt
     2401 cctcctaatc tatttttaat acattctcat tctgctacga aagattaaaa aattcaaaga
     2461 aagcgctcgc gaattatata aagtattggt atcattaata atttttttag aatcatacct
     2521 tataaattgt tattttcaga acaaaatgat atcgttatat taaaaccggc atcggttaca
     2581 ttaaaatttg gaggtaaaat atgaaaaaat tattgtttac agggttgctg gcaagttcag
     2641 cattgttttt attaggagga tgcggatcaa gtaatgcaag cgatgccagt tcaagtgcag
     2701 caaattcttc tgaaaaaaca agtgatggaa gaaaaaatat tgatttctgg tccttttggg
     2761 gatccggtgc acgaaaagaa gctatcgaag aaatcattga ggatttcaat caatcacaaa
     2821 acgagatcca tgtaaattat aagtatcagc catggggtga catctggact aaatctttat
     2881 ctgcaataac agctggaaat gcacccgatg ttattgtaca ggacataaat tccgttgccc
     2941 aaagagcaga agcacagcaa gcaactaatc tctccaaata tgtagaaaaa gacacttcta
     3001 aggattttta tccgcagctt tgggatacag tagaatatga aggtgatcct tatgcgatcc
     3061 cattcaatac agatacacaa gtcatttttt ataataaaaa gttatttaaa gaagctggaa
     3121 tcagtgaaca agatctaccg acaacttggg atgaattaga agaaacagca cgtaaactag
     3181 ataaaaaaga aggaaaagaa tttacacgca tcggttttta tccattatgg aatttagggg
     3241 ctgatgtatg ggcgctaaac gcagataacg ggactagttg gtttgacaaa gatgaaaaag
     3301 tcaagattga tactaaaaat aaagtggaag ccttggagtg gatccaagac tggcaagatt
     3361 attatgggaa aaatacgatc aataaattag aagccgaatt tgggtctggt gtatctgatc
     3421 catttatttc cggattagta gccatgcgtg cccaaaatat caattattac tctagtttga
     3481 tggaaaatgc accggatgat tttgattttg gcgtgatgca gctcccagaa aaagaagaag
     3541 gttcaggcca ttggtcgtgg ggtggcggat tcgtcttgga agtaccatac ggagcaaaag
     3601 atcctgaggc ctcttatgaa ttcatcaaat atctttcttc tccagaagta caagaaaaat
     3661 tcggcgaaaa aagttttgat atcatggcga gcaagacagc caatgagaat ttagtgaata
     3721 atgacaacct aacggataaa ggaaaaatga tctatcaaat ggcggacgaa aactttaaaa
     3781 atacggtgat cacaccagtt cctttagatg ctccggatta cacgacatta gtgaatgaac
     3841 agattgacca gattctttta ggaacaaaat caccaaaaga aggattggca gatgcacaaa
     3901 aagcagtaga aaacttagtc aagcaaaatc aataatccat ttttgactgt ctaggtacag
     3961 aggaggcaag aaaatttgaa gcgtttatct atccgaaaga aaaaagaagc aagatgggcc
     4021 tatttattta tctcgccttt tattatcggg ttcttggtat ttatgctagg gcctatgctt
     4081 ttttcagtta ttggtagttt caccgattat aatttgacat ccaagatgaa tttcattggt
     4141 ctagcaaatt tcaaaagaat gttcgttcac gatgatttat tttggaaatc gttatacaac
     4201 accgtttatt tcgtcatctt caatgtaccg ctgacgacga ttttcagtgt gtttttagcc
     4261 acagtactca atcaaaaaat caaaggaatc agtgtattcc gtacagcttt ctatttaccg
     4321 gcagtcttat caggagtagc agtctacatc ttatggatgc agctgctttc tccatctgca
     4381 ggattgatca acacatttct gggctggttc catatccctg gaccagcttg gctgtttgat
     4441 ccacaatgga cgaaacctgc gttgattttg atgaaaatat ggagttctgg aggagcaatg
     4501 ctactttact tagctacatt acaaaatgtt cctagatctt tatatgaatc agcagaaatc
     4561 gatggagcag gcgtttgggg aaagttcaaa agtattactt tgccattaat cactcctatt
     4621 attttttacg atgtgatcac ctctacgatc ggttctttcc aaatttttca agaagcttat
     4681 gtcatgacga aaaatggaac aggcggacct gcaaattctt tgctttttta taatctgcat
     4741 atgtggaaca aggcgttcgt agcttttgat atgggttatg cgatggctat gtcaatgatc
     4801 ctgtttctga tcgtgttggt tctgacgttt gtcaatttga agcttgcacc taagtgggtc
     4861 tattattctg gaggtgacgg caaatgatgg attatttcaa aaagaccagt gtgaatcggc
     4921 aaacaaaaaa aatactaagt tatgtcctca tgacagtaat aggaatcatt ttgatcattc
     4981 tattgctttg gatggtgttt acttcattga aaccaatgga agaaatcgtt cgttatcctc
     5041 cgaccttttt cccagaaaaa atcgtatggg aaaactatct ggatacgatt gccgcctttc
     5101 ctttttggcg ctatgcaaga aatacattat tcatcactgt gctagtagtg atcggtaatg
     5161 tcctttcaaa ttcttttatt gcttatggat ttgcgaagct tgattttcct gggaaaaagc
     5221 tgatgtttgc tttggttctt tctacgatga tgatcccggg gtttgtaacg atgatccctc
     5281 aatatgtatt attctccaag atcggctggg taggtactta tttgcccctg atcgttccat
     5341 cgttcttcgg caatgcgttc aatatatttc tgatgcgtca attttactta tcgatcaata
     5401 atgaattgat cgaagctgct gaaatcgacg gagcgaatca tttgtatatt tggagtcatt
     5461 tgatgcttcc attgacgaaa cctgccttga ttaccattgc gatcaattcc ttcaacgcag
     5521 cttggaatga tttcttaggt cctctgttat atatccaaga tcaagaaaaa tacacgttac
     5581 agattggctt gcaagttttt cagaaccaag caacgacaca atggaattat ctgatggcag
     5641 gagcaacact tgtcttgatc ccgacgattt tgctgttctt ttttgcacaa cgttatttca
     5701 ttgaagggat ggatttgaca ggaggttcaa aaggatgatg aatcgatcga ttggtaaaaa
     5761 agccaatgat tatttcttag aaatcattga atggattcca cgtcttatcc atttacagct
     5821 actatggttc ctgtgcgtct tgcctgtttt cacattagga actgcaagtc gaacagttct
     5881 gtatatgatt tatcattaca aaaaaaatga agagaaaaag ctcggttcac tattctggaa
     5941 aaagtttcgt gagaacttct ctctttatgg aaagcaagat agtttagcaa gtgtttattt
     6001 tcttcttcta ttgatcgact atcagatttt ccgctattgg ggtgggggaa ttggctatac
     6061 attgatgtat actacgttgt ttttgcttct attaagcgcg cttctcttta gttacaaaat
     6121 cttgcttcaa ctggaacaaa aagaagcttc ttggattact gcatttttct tgttttttaa
     6181 tgatttcaaa agcgcgcttt tctatttggc aggcacgttg atccttcttg tcgcattctg
     6241 gtttgcagga ccaatctatt ttgctttatt aggtttcagt ttcttgtttt attttcaagt
     6301 cctgcttttt atccatcgta cgcaagaaaa ggttcttaaa aaggaggaat aacatgttgc
     6361 gtttgattgc taaaatcgat gatagtatta tcaacaagtt cgccaagcaa cggaaaacgg
     6421 atagtcgaaa aatcaatttt ttgaaattta tcagtaatat ctctaatggt ggttttattt
     6481 ggttgctcct tgctgctgtt ttagtcttga tacctgatac tagagaatat ggtctagcca
     6541 ttcttatcgg cagtttgatc tatttttttg ttggtttgct ttttatcaaa aaaacgacaa
     6601 gacggaaaag accttatgaa aaaaatcgtc ggcttgtcct ttacacaagt aaaccgaaga
     6661 cttcttcttt tccttcagga catacagcgc tagccttttt atcagtagct gcgttgttcg
     6721 aattaagtcc agttgcagga atattagcct ttttatttgc ttgtttgatc gcttattcga
     6781 ggattttttt acttttacat tatcctactg acattttagg aggagctatt ttaggaagtg
     6841 ttattggcat cctatgcgct gccgttatct gaattaattg tacgaataaa gcatgagtgc
     6901 gtaaatgcgc atttgtgctt tatttttttg tttcgaggaa agcgtagttg tttattcatt
     6961 tgtcttaata acttaagttt tgttaaatga gaacgttata aaattttata atttaatt
//
LOCUS       DACEQH010000095         7015 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.099, whole genome shotgun sequence.
ACCESSION   DACEQH010000095 DACEQH010000000
VERSION     DACEQH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.099"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(3..455)
                     /locus_tag="IW833_002549"
     CDS             complement(3..455)
                     /locus_tag="IW833_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ1308606.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
     gene            605..1555
                     /locus_tag="IW833_002550"
     CDS             605..1555
                     /locus_tag="IW833_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1308607.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            1548..2507
                     /locus_tag="IW833_002551"
     CDS             1548..2507
                     /locus_tag="IW833_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1308608.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            2504..3259
                     /locus_tag="IW833_002552"
     CDS             2504..3259
                     /locus_tag="IW833_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1308609.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            3282..4235
                     /locus_tag="IW833_002553"
     CDS             3282..4235
                     /locus_tag="IW833_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1308610.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(4879..5175)
                     /locus_tag="IW833_002554"
     CDS             complement(4879..5175)
                     /locus_tag="IW833_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ1308611.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            5531..5887
                     /locus_tag="IW833_002555"
     CDS             5531..5887
                     /locus_tag="IW833_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1308612.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            5897..6796
                     /locus_tag="IW833_002556"
     CDS             5897..6796
                     /locus_tag="IW833_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1308613.1"
                     /translation="MGKLAIISDLHVDIKKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYEPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT         2132 a         1204 c         1420 g         2259 t
ORIGIN      
        1 ttttaaaata aacgatccca gaagcttttt ttcttttttg gtttttcttt ttcaactggc
       61 gaagagtttt cttctgggta ttttttttcg atatcaatca caggttcatt tactgcttct
      121 tttgctgtta agagcaatcc gaattcctca ggatttgcag tgacgtaatc gttgactaca
      181 gtaaatcggc tattgttttt cgttgctagc tggatatatt ggttttggac agaaaggggt
      241 agagccccat tcaagagtat ggaagcatct ggatgttttt tgagttcttt taaaaagttt
      301 tctttgtttt ttggttggat catttcagca acagtcatac taagatagca gcgttccctg
      361 aatgtcccta aatatttatg ctgttcttca gggttcacta aaggtgtacc ataaagtccc
      421 ttatccaaat ggctttttac atcatcttgt gccataaaaa ataccctcct tcttctcttt
      481 gtctttctta cgattatagc atattcccaa taatacctaa aacatatgaa aaagcggttg
      541 cttttgttca acgattcact taaaataaga atgaaaatca ttctcaatta gaaaggtgga
      601 ttggatgcgc tacggacgac tagtagcatt attagttatt ttaagtagta tttcattgtt
      661 catcggagta tcgaatgtac aattgtcaga actgctccag ttagatgggc aaagcttaca
      721 aatcttgcta gtgactcgtt tgccacgaac gatttgtttg atccttgtgg gagcgaccag
      781 tagtatttgt gggttgatca tgcaacattt gacccaaaat aaatttgttt caccaacgac
      841 tgcgggcacg atggatagtg cgcgtttagg tattttagtg gctatgattt tcctacctgg
      901 tgcatccttg attgtccgat ccttgacagc attttgcttt gcttttgcag gaacattgct
      961 ttttttatcc cttacgcgat tgttccccca gaaaaatcaa ttgatgcttc cgttgattgg
     1021 agtcatgtta gggaatatca ttggttcagt tgctactttt tttgcttatc aattccagtt
     1081 ggtacaaaat atgacttcat ggcttcaagg aaattttgcg actgtgatac gaggaaatta
     1141 tgagttattg tatgctgttg ttccattgtt gctcttggtt tggctatttg cttaccgatt
     1201 tacgatcatt ggtctgggcg aatcgtttgc agtaaatttg ggaatagatt accagaagat
     1261 gcagttttta ggcattggct tagtatcatt ggctagtgca gtcatgttga tcatggtagg
     1321 aagcattcct tttatcggag tgatcattcc gaatcttgtc tctagaatat atggagatca
     1381 agtgcacaaa acaattggcg taactgcttt agcaggtagt ttgttcttag tattttgcga
     1441 tcttgtcgct cgcgtgttga tttttcctta tgaaatacca gtcagtgtga tcgttggaat
     1501 catcggtggg gctatcttcc tttatcttct gataaggggg cgacgatatg cttaaaaaaa
     1561 agccatctat ttttattggt atgcttgttc ttgttttatt tgttacagga atgtacttga
     1621 cgatccatac atatggaaac tggcattttg catgggcatt gaggagtaag aaaatttttg
     1681 catttatcct tgtctcgcta gctgtcagca gtgccacaat cagctttcag acaattacgc
     1741 ataaccagtt tttgactcct tcagtattgg gacttgagtc gttatatgtc cttgtccaaa
     1801 cggtcctttt cttctttatc ggaggagtga cgatgcttca gcagacaggt acagtttttt
     1861 tcttattgaa cgttgggctg atggtcgggc tgagtctatt gttatttttc tttcttttga
     1921 aaaaaatgag agcgaatctt tttttattac tgatgtcagg gatgatttta ggtacgcttt
     1981 ttggtagtgt cagtaccttt ttacaagtca tcatggatcc gaatgaatat gatttgcttc
     2041 aaggcagatt atttgcaagc tttggcaata tccagagtca gtatcttttg cctgcttctt
     2101 tgatcattat cggctgctgg tacctgttat ttcgatatcg acatgtgcta gacgttttcc
     2161 atctcggaaa tgaacaagca gtaaacctcg ggatagacac agatcgtttt gaatttttga
     2221 ttttatgttg tgtcagtctt ttgacaggga catcaacggc tcttgtgggt ccagttactt
     2281 ttttagggtt tattgtcgca aatatcagtt atcgtgtctt ttctacttat cgtcatggtc
     2341 agctatttat cggaagcttt ctggtagcta tgctgtttct gacaggagga cagctcgcag
     2401 tagagcagat tttcggctgg aatacaacag tgagtgtcgt cgttgaattt gtcggaggaa
     2461 tttattttgt cacaaaattg attcgcgaaa ggaagagtat cctatgattg atttacaaaa
     2521 ggtcaataaa ctgtaccatg gaaaatcagt ggttaaagat gtacagttaa cgattcctga
     2581 aaagaagttg actgctttta tcggtccaaa tggtgctgga aagtcaacgg tgttgtcgat
     2641 gatcagtcgt ttgataccaa aagatacagg tgaaatctat ttagaccata acgaagtaaa
     2701 ggcttggaaa tccaatgaat tagccaaaaa attatcgatc ctacgacaat cgaatgaaat
     2761 aaatgtccaa atcaccgtga aagaattagt agaatttgga cgatttcctt atacgaaagg
     2821 aaaattgacg acaaaagacg aggaaatcgt tcaggaatca ctggaacacc ttgggttaga
     2881 agaaatagcg gatcaatcga ttgacactct ttcaggcggt caactccaac gtgcgtatat
     2941 tgcaatggtg ctagcgcaag acacggatta tattttactg gatgaacctt tgaacaatct
     3001 agatatgaat tttgcggttc aaatcatgca gatcttaaaa aaattagtac aagaaatcgg
     3061 gaaaacaatt gttattgttc ttcatgatat caactttgct gcaagttatg cagacgaaat
     3121 tattgcaatg aaagatgggc agttattcac gcaaggaacg acccaagaaa tgataaaaaa
     3181 agatgtactg gacgaattgt acgaaatgga catacgaatt tgtgagattg aaggtcgcag
     3241 attctgtctt tatttctaaa actaacatta ggagagaaga gatgaaaaaa ttagtagtag
     3301 gtatcttgat tctagcatca tttggtttag cagcatgtgg aaatagtgac aacaccagtc
     3361 aggcagatac aaagaaaagc agtacgcaag ctgaaacggc attaacgata aaagacagta
     3421 atggcgatca aatcgaggtg ccaaagaatc ctgaaaaagt cgtcgtattc gacaatggtt
     3481 ctttagatac gatggatgct cttggtgttg gagataaggt agttggagca gctacaagca
     3541 gccttcctga gtatctttca tcctataaaa aagtggaatc agcaggtggg atcaaagaac
     3601 cagacttaga aaaaatcaat cagcttcagc cagacctgat catcatttct ggccggcaaa
     3661 gagattttca aaaagatcta agtcaaattg ctccgaccat tttcctttct ttagatgcga
     3721 aaaatccatg ggaatctttc cagcaaaatg tcactgcttt aggggagatc tttggtaagc
     3781 aagaagaagc taaaactcaa ttagaggaat tatctagtgc aatcgatcag acgaaaaaga
     3841 aagcagaagc tactgacaaa aaagcactag tcacacttgt gaatgaaggg caattatcag
     3901 cctacggatc tggttctaga ttcggtttca tccatgattt atttggcttt gaacaagcag
     3961 atgatcaaat cgaagcatcc actcacggtc aaagtgtttc ttatgaatat gtgctggaaa
     4021 aaaatccgga tatcttattt gtagtagaca gaacaaaagc tattggtgga gacgacagca
     4081 aagatgatat ttctgccaat gaactggtag cacaaacgaa tgctggtaaa aatcagcaga
     4141 tcatctccct tgaaccagat gtctggtatt taagcggggg cggtttagaa tcaatgaaac
     4201 tgatgatcga agatgtgaat caagcattta aatagcataa ataacagagg attaaactct
     4261 catactatgt aacaataatg tttcttacat tcttaaatag caagagccta tcctcaaaga
     4321 agttatcaaa aaccatccgt aaaatcgtat tgatgatttt acggatggtt tttatttatt
     4381 tgggaaatct tttttcaacg tgaattcatt ctctacatca tacgggttga ttctagaatg
     4441 gggagtggca aaaaatagtg tgatccattt tttcagcatc aagatcgcct cctttgattt
     4501 gaatcaaagg tacaaagcga aacttagata aaacttaaat ttagatattt ttattttgta
     4561 ccgatctctt ctttaaacga tgttccatct gctgcttctc ggcaataagt ccactagact
     4621 agaaaatatg agaagctatt ttctgtccag cgttcttatt actcaaagca aatcgcagtt
     4681 gtcacaatct cttctaaacg atgttccatc tgctgcttct cggcaataag tccactagac
     4741 tagaaaatat gagaagctat tttctgtcca gcgttcttat tacttaaagc aaatcgtagt
     4801 tgtcacaatc tcttctaaac gatgttccat cagctgacct tcggtattag ctcaaagcac
     4861 tgatttcaca acctctattt aggcatgtgt ctcagcaagc ggttgatagt tgatgatctg
     4921 catttttcga taaaaatcgg cgggaagctt actccaccgt tttaataatt gatagaaata
     4981 actctcttct gctttgattt tatactcgct taatatcaat ccaaaactat tcgttttttt
     5041 tgaagctaat tttttataaa tatccaatgc tttggtttta tcttcattga caaagagtgt
     5101 ttttacttca atataagaac cctcgatatt ttgtgtttct tcgataaaat agatgttttt
     5161 tatatattcc ttcatagtga ttcctccaaa gaagtctttt tatttgataa gtttactcta
     5221 ccgaagagtt gttaataatc cgtgaacaaa aagtaattct ttttccaata gttgttaaag
     5281 gttttttatt ttttatagta ttttttgtta tttaagaaag gaataaggtt aaacaaataa
     5341 caggttgctt tttgggtctt tttccgttca agaaactgaa cgaagaaaag ggaaagttgt
     5401 ctcttttgtg ataagagaaa tcataataaa cgaatgactt actttccaat aaattgagaa
     5461 cagtcagaac cagacaaatt agttgcatct gatgaataaa gtgattattc tatgacatga
     5521 ggtgatacag atgaaattag aagtgacaaa agaagcacaa gaagtattga agaataaatt
     5581 agaagcagac gatcaacttt tattagatat cgaaaacggt gacggtccat ttgcagaaag
     5641 ccaagtcagt tgccaattgg acacggcatt tcgattgatc attgtcaaaa aagatacaca
     5701 aacagatcta agtctttatt ctgtagtggc tgatacacca gttggaccaa tcaaaatcaa
     5761 agacagtgca atactttaca tggatgatcc atccacactt gctttagaac caacttataa
     5821 tagtttacaa ttaaaaggac caagcggctt gagaaaaggc aacttgcaag ttgttcgaaa
     5881 agactagagg gataaaatgg gaaaattggc gatcatcagc gacctacatg tggacatcaa
     5941 gaaattcggc gaatcagagc tgatgcagct ttggcaagtt ttgcaaaagc acaagatcac
     6001 tcggcttcat ctagccggag atacggctaa taaagtagac cgttgcctag ctgttgtcga
     6061 atttttcgtc aataaaggat tatctacgac atttcatttc ggcaatcacg agttggcaga
     6121 tgtgcccaat gaacagatga tggaccattt tccagaccca cattttttaa atgaacggta
     6181 tattgcttta aatgagcaga ccgttctttt agggatgaat ggctggtacg actatcagtt
     6241 ttccgaattg aaagattacg aggcaatcgt acgaatgaag cgtttgtatt ggtatgatcg
     6301 gttcatccag cgtgggaaaa cagatctcga ggtcaatgca gaaatgcttg cagctgcaaa
     6361 aaatttgcta gaccagctgg aagcaaaaaa acttgaggtg attttatcca ctcatttcgt
     6421 tcctaaacgg gcatttatca tgtatcaatc tgctccttat gagcgctgga acaaattaaa
     6481 tgcattttta ggatcagaat cattcggaga attacttgac cgttattcaa atatcaaaca
     6541 agtagttttt ggccatacgc atcgtcgctt tgaagataaa tccatcggtc acacgattta
     6601 cagctgcaga ccttttggtt attattatga atggcagctg acccgagatt ttgtcttgaa
     6661 agaacagctg ttagatagct atgaacccat gaaattacgt gtcctcttgc gtgcccatca
     6721 tccggccttt caagcctatc aaacacaaca tttgcaagag gaatttgaac aggcgatgac
     6781 aatcgttccg tattgagaca gcattagtaa tgtgtaaaag aaaatacctt gtaaaacata
     6841 aggataagta agagcagtag atccatttat cgtggatatg ctgctttttt tgtggaaaat
     6901 aatcagcgaa tgtttatttg atcggatgat ttgtaactcg cagagtacta gatgcaagcg
     6961 tttattcttt ttttgaacga taatcacaaa aaaaaatata atatacattt tttat
//
LOCUS       DACEQH010000096         6901 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.101, whole genome shotgun sequence.
ACCESSION   DACEQH010000096 DACEQH010000000
VERSION     DACEQH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.101"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..182)
                     /locus_tag="IW833_002557"
     CDS             complement(<1..182)
                     /locus_tag="IW833_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1308614.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="IW833_002558"
     CDS             517..951
                     /locus_tag="IW833_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1308615.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="IW833_002559"
     CDS             974..1294
                     /locus_tag="IW833_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308616.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="IW833_002560"
     CDS             1435..1626
                     /locus_tag="IW833_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308617.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="IW833_002561"
     CDS             complement(1697..1954)
                     /locus_tag="IW833_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ1308618.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="IW833_002562"
     CDS             2112..2375
                     /locus_tag="IW833_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308619.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="IW833_002563"
     CDS             2399..2587
                     /locus_tag="IW833_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308620.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="IW833_002564"
     CDS             complement(2670..2855)
                     /locus_tag="IW833_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308621.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="IW833_002565"
     CDS             3070..3441
                     /locus_tag="IW833_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1308622.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="IW833_002566"
     tRNA            complement(3591..3663)
                     /locus_tag="IW833_002566"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="IW833_002567"
     CDS             complement(3709..4137)
                     /locus_tag="IW833_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308623.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="IW833_002568"
     CDS             complement(4197..4955)
                     /locus_tag="IW833_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ1308624.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="IW833_002569"
     CDS             5250..5447
                     /locus_tag="IW833_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308625.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="IW833_002570"
     CDS             5553..6035
                     /locus_tag="IW833_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308626.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="IW833_002571"
     CDS             complement(6093..6689)
                     /locus_tag="IW833_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1308627.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2338 a         1060 c         1355 g         2148 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc cccagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt atttgaaaaa
     6901 a
//
LOCUS       DACEQH010000097         6453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.102, whole genome shotgun sequence.
ACCESSION   DACEQH010000097 DACEQH010000000
VERSION     DACEQH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.102"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(95..550)
                     /locus_tag="IW833_002572"
     CDS             complement(95..550)
                     /locus_tag="IW833_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308628.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(704..1912)
                     /locus_tag="IW833_002573"
     CDS             complement(704..1912)
                     /locus_tag="IW833_002573"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ1308629.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2024..2356)
                     /locus_tag="IW833_002574"
     CDS             complement(2024..2356)
                     /locus_tag="IW833_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1308630.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2504..3379
                     /locus_tag="IW833_002575"
     CDS             2504..3379
                     /locus_tag="IW833_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1308631.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3461..4042)
                     /locus_tag="IW833_002576"
     CDS             complement(3461..4042)
                     /locus_tag="IW833_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ1308632.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4048..5307)
                     /locus_tag="IW833_002577"
     CDS             complement(4048..5307)
                     /locus_tag="IW833_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1308633.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5309..6355)
                     /locus_tag="IW833_002578"
     CDS             complement(5309..6355)
                     /locus_tag="IW833_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308634.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1799 a         1334 c         1100 g         2220 t
ORIGIN      
        1 tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat aagacacatc
       61 tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc ccgtatacat
      121 cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt tcgctgattt
      181 ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat gggaagtaca
      241 caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct tcgtatttca
      301 atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt gtcgcaactg
      361 cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt tcaacaaatt
      421 cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga tcgccgatat
      481 gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc gctgtcattt
      541 ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac ctcagtttag
      601 cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt atctgcccac
      661 tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc aatttttctt
      721 tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct acgacggctt
      781 tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca gtttcttcta
      841 gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa cgatcatagg
      901 attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat tcttctactt
      961 gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct ccagtttctc
     1021 gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta gaacacttcc
     1081 acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg atcaataatg
     1141 cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa cgtaaaagtg
     1201 cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca ataaatgcat
     1261 ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca tttatacgtt
     1321 ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag gtttcttcct
     1381 ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt aaggcatctt
     1441 catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct agatcttgca
     1501 gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca ttgatcgtaa
     1561 aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga tctggtctac
     1621 ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt tcatcatttt
     1681 ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga aaaagctgtt
     1741 tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc tggttcagca
     1801 agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc tctaattttt
     1861 tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc atttttccaa
     1921 cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga ctgtttgaca
     1981 agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg tccttacgct
     2041 caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa acttcagtta
     2101 agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca ccaagttctt
     2161 ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa gtgtggttga
     2221 cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct aatggagaga
     2281 aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata ctgtataaag
     2341 aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt aacgcaaatc
     2401 ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag gaatttctaa
     2461 atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca aacgaaaaac
     2521 agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg gtcttgttta
     2581 cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct tgatcttaag
     2641 agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc cattgatttt
     2701 actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag gtacactttc
     2761 tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt tacagcatga
     2821 tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg ggatcgtcta
     2881 ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag aacaaaatta
     2941 cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac ttgcttcatt
     3001 aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg ttttcagtcg
     3061 tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg tcgtctccaa
     3121 caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg tgactttcct
     3181 ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg tcgttagctc
     3241 tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag ctttcctttc
     3301 aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc cacagacaag
     3361 attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag attccacatc
     3421 cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta tgttcactta
     3481 cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca tcagataatt
     3541 tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca taaagcattt
     3601 ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca gtatcttctt
     3661 cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat agatattctc
     3721 ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc caattcaaac
     3781 ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa aacaatttca
     3841 tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca aaattcgccc
     3901 cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa ttcaagatcc
     3961 aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa aaattcttct
     4021 tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta ataatgacag
     4081 tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt gcgctctttt
     4141 cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg gttcatgtga
     4201 tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt ctaattcatt
     4261 ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca tctgattgat
     4321 cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta aagtaagtaa
     4381 tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt ctgctcgctg
     4441 tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga cttggtaagg
     4501 attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt cttcttcttg
     4561 aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt ctcgaagttc
     4621 ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa aagctagctg
     4681 gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta cgatagctgc
     4741 ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg agattcctga
     4801 aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct ctttgaaacg
     4861 atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat caggaagcaa
     4921 cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa cttggtacaa
     4981 atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca ctttttccag
     5041 gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta acgggatgtt
     5101 cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt ttgcttcgat
     5161 caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg ctgggtcttc
     5221 ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg ataaagcttg
     5281 caacattttt tcgctatttg tattcatttc aattccctct tatctcactg acgatttgtt
     5341 gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat ccgtctatta
     5401 tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa agaacttttt
     5461 ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca agcaagtcca
     5521 gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga ttccacacga
     5581 aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg ccattttcgg
     5641 tcagataaaa agcttgttca aacagtttac cataatttga caggttttct gcctctaatt
     5701 gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt aattgctttt
     5761 tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc attccgcaac
     5821 agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg attgtttgat
     5881 cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt ggtagctgca
     5941 aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca gggatcatcc
     6001 atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc atgatccctt
     6061 catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg acgaaatcaa
     6121 gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata taccagtcat
     6181 tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca taatagcgct
     6241 gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga ctgactagcg
     6301 tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt tccatacgca
     6361 tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa agataagagc
     6421 aaagctcacc tcataacttg gaaataaata aaa
//
LOCUS       DACEQH010000098         6418 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.103, whole genome shotgun sequence.
ACCESSION   DACEQH010000098 DACEQH010000000
VERSION     DACEQH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.103"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(99..1229)
                     /locus_tag="IW833_002579"
     CDS             complement(99..1229)
                     /locus_tag="IW833_002579"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ1308635.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDRLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IW833_002580"
     CDS             complement(1878..3080)
                     /locus_tag="IW833_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1308636.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IW833_002581"
     CDS             complement(3140..3343)
                     /locus_tag="IW833_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1308637.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IW833_002582"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IW833_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IW833_002583"
     CDS             4880..5692
                     /locus_tag="IW833_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1308638.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1926 a         1313 c         1009 g         2170 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ctatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatag
//
LOCUS       DACEQH010000099         6246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.104, whole genome shotgun sequence.
ACCESSION   DACEQH010000099 DACEQH010000000
VERSION     DACEQH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.104"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            97..324
                     /locus_tag="IW833_002584"
                     /pseudo
     CDS             97..324
                     /locus_tag="IW833_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            450..1421
                     /locus_tag="IW833_002585"
     CDS             450..1421
                     /locus_tag="IW833_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1308639.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICKENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            1561..2874
                     /locus_tag="IW833_002586"
     CDS             1561..2874
                     /locus_tag="IW833_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1308640.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            2917..3783
                     /locus_tag="IW833_002587"
     CDS             2917..3783
                     /locus_tag="IW833_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1308641.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLMPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            3780..4628
                     /locus_tag="IW833_002588"
     CDS             3780..4628
                     /locus_tag="IW833_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1308642.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            4642..6114
                     /locus_tag="IW833_002589"
     CDS             4642..6114
                     /locus_tag="IW833_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ1308643.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            6131..>6246
                     /locus_tag="IW833_002590"
     CDS             6131..>6246
                     /locus_tag="IW833_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1308644.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEET"
BASE COUNT         1956 a         1061 c         1349 g         1880 t
ORIGIN      
        1 taaaattgaa aacacaagaa tactaataaa tcaacgattc ttgtgttttt aatttttcac
       61 taccgtgcat tttcgtttct atgttccatg taccccaatc ttgaaaaatt aattaaagac
      121 ttagataaag ccattgacga cttagtcgtg gtgctaccgg agtatcaatg tttaaccagt
      181 atcccaggta ttggtcctgt ttaggctgct ggaatactcg ctgaaattgg tccaattgaa
      241 cgtttttctg atgaaataaa attgacaaag tatgccggcc tatattggcg acagagtcaa
      301 tccggtaaat ctgaatacga aaattttttt acgaaaagct tcacgtctaa tatctatttt
      361 aaaataaaaa tattcctgct aatttggaat cgtttccttc ttttggtata ataatacctg
      421 aaattctttt gagaggagtt gttcgtgaag tgaaaactac tatgaaggat gttgctgcgt
      481 tagcaggagt tggtgtgggt acggtatcta gagtaataaa tggagtaaag gtgaaagagg
      541 ttaccaaaga aaaagtaatg gctgcaatta gtgagcttaa ttatgagcct gatgaatatg
      601 ccagaggtat gaaaacgaat agtagcaata caatagctct tattttacct acgatttggc
      661 atcccttctt ttccgagttt gcctactatg tagaggaagc tctcagccaa aaaaattata
      721 agcttttttt gtgtaatgcg aacggcagtg cggaaaaaga aaaagaatat atccaaatgg
      781 tcaagcagag taaagtagac ggaattatag gtattaccta ttcagacatt gataaatatg
      841 tatcatcaaa tttaccattt gtcagtatag accgccattt ttcggaacag gtttcttatg
      901 taacggcaga taattattta ggtgggcaaa ttgccgctca agaactactt actcgtaact
      961 gtaaaaactt gtgctatgtt gggggtgttt caccttaccc taacgaaaca aacaatcgta
     1021 aagctggttt ttacaatatt tgtaaggaaa ataaaatagc tgtgaaggta ttggatatgc
     1081 ctgaaccaat tagcaattta gaggaacaat tgctcgcttt tttttcggat aatcctgaaa
     1141 ttgatagcat ttttaccatc aatgatttca tggctttgga tgtgataaaa gcccaaaaaa
     1201 aactagggaa ggaagcacct aaggattttc aaattattgg ttttgatgga gtcaagctat
     1261 cgttgcaaaa agactattta ctatcaacga ttgtacagcc gatagaggaa atggccaagg
     1321 tttcagtaga aacactcatg gcgatgattt taaatgaaga accgcctaag cgtgtggtag
     1381 taccggtttt ctttcacgct ggagagacga ctaaagagta aaaaaccggt ttataacgat
     1441 ttttttgaga taggttgaca gaaagtgatt tcaatgatat cttaaaacca attaattgga
     1501 aacgttacca attaattgaa aaataaaacg gaaacgtttc aaaaaatagg aggaacatcg
     1561 atgaaaaaga gaatgttagc aattgcagca attttagcat taacagctgg aggacttgca
     1621 ggatgtggca gtaaggagaa aagttcaggt agcagtgatc caggagaaat taagatttgg
     1681 gttcagtttt ctgatgaaac gcctgaagga aaagcttggg cagagattgt gaacaatttt
     1741 aatgatgaat acgagggtga atataaagct gttactgagt atattcctcg tagcggaagc
     1801 ggcggtggat atgaggataa agtaaatgct gcaattacaa ccaataccct tcctgatgtt
     1861 attacgttag atggaccgaa tactgcagct tatgctcatt caggagtcat tgctccatta
     1921 gatgattatt tgaaagatgc ggacatggat gatgtattag acagcattat tcagcaagga
     1981 acctacgatg gcaaaatgta tgcgtttggt ttctcagagt ccagtgtggg tgtctattac
     2041 aataagtcgc tgttcaaaga tgctggaatt acagaagatc aattgccgac gttagaagat
     2101 ccttggactt ggacggaatt tcaagcaatt tgccaaaagc tgactgataa atacaaaacg
     2161 cctgcaattg atatgcaaat ggcgtcggga gacgaaatgc tgacctacgc atatacacca
     2221 tttatatggt cgaatggcgg cgatgtcgtt aatgctgatg gaaccaaagc cgaagactat
     2281 tttaacggta aaaaatccgt tgaagcgttg gaatttattc aagaattggt tgaaaaaggt
     2341 tacaccacaa tcagtcctat tgaaaaagga tttgaaactg gcaaatatgc catgatgctg
     2401 agcggttctt ggacaatggc agatttagag gaaaactatc aagagattga ttatggtatt
     2461 ttgccttacc ccgtttcaga tgcaactggg gagttagttt caccaaccgg aagctggcag
     2521 ttagcaatga ctgagaaaac agagaaaaag gatgcttctg cggcctttat taagtttgca
     2581 accagtacag aatctagtcg tattatgagt ttagggaaca gtgttctacc tatcagaaat
     2641 tcaacagttg atttaattaa agatgaagtg tcggaaggaa tgcaggtatt aattaagcaa
     2701 aaccaaattt ctggacatgc ccgcccagtg gttgtagctt atccgcaagt atctagagca
     2761 ttccaacaag cagttcaaga tattagctac ttcgaggaaa acccagatgt acaaaaagta
     2821 gcagatagtc gcgctgctga aatgcaacag gcaattgata cttcactaaa atagaagttt
     2881 ctaaaggggg ccaatcaagt gttaaaaaaa ccgacaatga aagacaattt gataggttac
     2941 ggctttctag cgccagcttt gctgctgctg acgatttttc tgatcatacc cgtaatcatg
     3001 gtaatttatt atgcctttac tgattattat ttgttaatgc cagatgctag acaatttatc
     3061 ggcttagaaa attttaagcg actgttcgct gatccaattt ttgtaaaaag tatttggaac
     3121 acggctaaat ttgttgtttg gattattccg gtccaaatag gggcagcttt aggaatggca
     3181 cttattatca acaaaaatcg taaaggaaat atctttttta aagtagcctt ttttgcgcca
     3241 gtcgtgatgt ccttagtcgt tatttcgatt ttatggttgt acttgttaaa tcctaatgaa
     3301 ggtctggtta atacgctatt tgcaaagatt ggaattgctt cgcagccttt tttgactagt
     3361 cccaaacaag caatgtatac gcttgtgttc gtatcggcat ggcaaggtgc aggatatcag
     3421 atgctgctgt ttttaggggg aatgcaaaat attccgcaag acgtttatga ggcagccgaa
     3481 attgatggtt tttctaagtt tcagcaattt cgctatatca ccatgccatt attaaagccc
     3541 acggcgattt ttattttact taccacattg atttcggcct ttaaactaat cattcagcca
     3601 atggtaatga ctcaaggtgg accaatgaat tctactatga caatggttta ttacatttac
     3661 caaactggat ttacagaccg tatggtaggc tactccagct ccattgcgtt aatctttacc
     3721 actatgattg gtttaattac aattgctcaa agaaaattgg taaaggagga tgatccagca
     3781 tgaccaagaa atttggaccg ctaatgatcg tagaatattg tttgctgttc cttttagcgg
     3841 cattattcat tttcccgatg ctgtggatgg tagtttcctc tatgaagcct gaagcagatg
     3901 tctatcaaaa tttagcttcc ttcaaagctt ttttacctag tttgaatcca gctaattggt
     3961 ttaaaactta tcaagaggtt atttcccggt tcagtgtggg aacttattta ttaaacagta
     4021 ttttttatgg tttaaccttt gcctgtgggt caatcattgt aaactctttg gcgggttttg
     4081 cctttgcgaa ggttaatttt tcaggcaaga aaattatctt tggagctttg ttagctctgc
     4141 tgattgtccc gatggagacg gttttaattc cgcaatttac catcgttaat tcactaggct
     4201 tagtgaatac tcgcttggca gtgattttac cagcgatggc cagcgcgttt aatatctatc
     4261 tgtttagaaa tttctttatc gcgattccag aagaaatcat tgaatcagcg aaattagatg
     4321 gtgcaaatat tgttaaaatt ttctttcaca tcatgctgcc aatgtctaaa ccagccatag
     4381 ccacagtagg tgtcttatcc tttatctcca gctggaatga ttatatttgg ccgttaatgg
     4441 tcttaactga cagcagcaaa ttttcaatgc aagtggcaat tactacgatc aatactacac
     4501 agccagttta tatcaatcaa gtgatggctg ttttaaccat tgcaacgatt ccactgatta
     4561 ttgtttatat cgttgcacaa aaatatattc tgcagggact tggcggttct ggaacgggta
     4621 taaaatagga gtgaatagtg aatgttgcaa aaacttttaa aggccaatga gtttattgag
     4681 aaaaatgctc aaacagtcaa tcaagaatac cggaataaat ttcatttgat ggctccgatt
     4741 ggctggatga atgatccaaa tggttttgtt tattatcaag aggagtatca cttgttttat
     4801 caatattatc cttacgacag catctgggga ccaatgcatt ggggccatgc taaaagcaag
     4861 gatctaattc actgggagca tttgccagta gcactggcac caggtgaaag ctatgacaag
     4921 gaaggctgtt tttccggcag tgccatagaa aaggatggca agctgtattt gatttataca
     4981 ggacacaatg ttgtggaagg gcaagttcga caagttcagt gtttggcagt ttcagaagat
     5041 ggcattcact ttgaaaaata tgccggaaat ccaatcatcg cagaagatca cttagcttct
     5101 gttgcaacga ccgaagattt ccgcgatccc aaagtgtttc agcgaaaaga agactatttt
     5161 actgtagtcg ctactaaaac tgcagatgct cgtggacaaa ttgtgatgtt ccattccaaa
     5221 gatttactaa cttgggagtt ttattccgta ttgctggagg gaactccaga acaaggcatt
     5281 gtatgggaat gcccagactt gtttgaacta gatggaaaag aagtcttgat tctttctcct
     5341 attcaaatga ctccacaagg caatgcttac caaaatacga actcaactgt tgctttcatc
     5401 ggtactgtcg attggcaaac aggcaaattt ctagtagaga attttcatga aatcgatggc
     5461 ggtttagatt tttatgcacc ccagacctgt gaaaacgaag caggccaaag aataatgatt
     5521 gcttggatgc aaatgtggca gcgtaaaata cctacgaatg atctgggcca tggttggtca
     5581 ggttccatga ccttgcctag agagctgagg gtggctgata atcaattgtt gcaacggcca
     5641 gtttcatcaa tttatcaagt aattgatccg caggaaagga ttgaatgggc gaagactgaa
     5701 gagaatcgct tcttactgaa ggacttagta tatgaccagc actatttacg cttggaaatc
     5761 aatttaaaac aggcaacgaa actagaactt cactatgcga agggacccga ccatacatta
     5821 ctgcttaact atgatgttga acgggagtgc tttactgctt ccagggaaca catgccgtat
     5881 gatttatcag gagctgaaaa ggaagtgttg aaaaagcgca gtgtgacgat tcctttggaa
     5941 aataattgtc taaccatcga aattttccgt gatacttcag ctattgagct ttttttaaat
     6001 gatcgtctga ctatgagtac aactttttat gagacagaaa aaggacagga ttttcttttg
     6061 ctggcagaca gtgaagtaac cttttcattg gaaagcggct taattaatct atagaaggga
     6121 gagctcagtg atgatttatg gttcaatcga agcaggcggc acaaaatttg tgtgtgccat
     6181 tggcgatgaa gaaatgacga tcaaggaaag agtgagcttt ccgacaacga cacctgagga
     6241 aacaat
//
LOCUS       DACEQH010000100         5801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.105, whole genome shotgun sequence.
ACCESSION   DACEQH010000100 DACEQH010000000
VERSION     DACEQH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.105"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(28..267)
                     /locus_tag="IW833_002591"
     CDS             complement(28..267)
                     /locus_tag="IW833_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308645.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            complement(381..722)
                     /locus_tag="IW833_002592"
     CDS             complement(381..722)
                     /locus_tag="IW833_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ1308646.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            complement(709..1038)
                     /locus_tag="IW833_002593"
     CDS             complement(709..1038)
                     /locus_tag="IW833_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308647.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            complement(1629..1961)
                     /locus_tag="IW833_002594"
     CDS             complement(1629..1961)
                     /locus_tag="IW833_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ1308648.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(2798..3883)
                     /locus_tag="IW833_002595"
     CDS             complement(2798..3883)
                     /locus_tag="IW833_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ1308649.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHKAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            complement(4203..4610)
                     /locus_tag="IW833_002596"
     CDS             complement(4203..4610)
                     /locus_tag="IW833_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308650.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(4629..5447)
                     /locus_tag="IW833_002597"
     CDS             complement(4629..5447)
                     /locus_tag="IW833_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1308651.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT         1741 a         1053 c          785 g         2222 t
ORIGIN      
        1 agcctatgat cacatacgct ctacacttca gtcttgggtt atctttttct ttcgtttaat
       61 tactggccgt gctggtttca aatccttgat tccattatgg gttctccaat aatcgaattc
      121 ttttcgtaat ctttcttttt ccatatctgt tcctttagac agggatttga atacatcttt
      181 aggtgaataa ccacgagcta acagctgtac ataccttcct tttgtcaaag tatccatatc
      241 catcgcctct aaaactggtc gcgccatcta attttcctcc taaataaatt ccttactcaa
      301 tgcctaaggt aatactacct ttttattatt cgtcgtgact ccttctaagc ctttatctgt
      361 aaaatttatt cctccagttt ttattcttct ttatccgcta caaattcaat gatttcaacg
      421 ctgacaatat aatcactcaa atatgaccaa gtatcttcaa cagataattc gtctccatta
      481 tcatccgtaa gcgtcatttt aaaccattca ttaccttcat catcttcagg tagcaagccg
      541 aatccaattg gattatcagt caatgtttga tcgtataaat catcctcgtc taatgctaaa
      601 acatctaatc ctttacaatt tccgcctctt ctaaacgtca caaatccttt gtaatcccaa
      661 cgttggaaag aaaccttaat cgtatgtgtg cccaatcgta tattcggatc ataatttttc
      721 atttgttgta acctcctcat aatcagttag tactataaag gtatctttgt ttttatccaa
      781 aaaatctaat aaatcttttg tagaagatat ctcttcatca acttcaccat aataaatacc
      841 ttcgataatt gttgctatat ctgaaaaact aatttttact ggacatttat cgtctaccgc
      901 attcaatacc gacaaaattg cttctttttc tgcaatctct cgtttgaatt tctttggagc
      961 taatcgcatg acactcaagt cattttcaag cttttcaatc catgcagttt tactcatttt
     1021 cgcaaatttt tgaatcattc ctctaccacc tttgctatct actaaattag ttttacttca
     1081 taacgaatat ctttatcgtt tttcgttcac tcaaaaatat ataaaataaa tcgactcgtt
     1141 tatggcgtcc gaaacgagaa accctagtag acagcggtaa atgctcgatt gatccaatga
     1201 tcaacgtcct cgcaagtatc tacatgtact gcagcacacg acacaatttc ttgtgcgtcc
     1261 tccccattac gagaagacct actccttatt ccatgagttt gcgtcaaagc ctagatccct
     1321 tagtagctgc tcatgattaa tctaggcgct attctaactt gtctaacttc taaattccct
     1381 cttgtttact ctgtgcatat tatttacaca aattcatttc accactatcc aaacaagggc
     1441 atttactcga ttgaccaatt ttttacgtta ccaatcgtaa aaacctaccc cgtcactccc
     1501 cgaagggaat tgttgataga tcaagtctac tccttatttt tctagtgatt ctaagttcct
     1561 ttagaagtct cgacaatatt ttcaagcaag cgaaaaacga taaaatacaa gttatgagaa
     1621 tgatcaattc agctgattaa acgccccatc ccttttgcgg tacaacgcaa gactccattt
     1681 tttcttcgac caatcagtac accgttttgg tcataaacat gtttgattgt tggatatagc
     1741 tcttttaaag ggataggatc ttttaaaatt atttgttttt gtttcccaat gtaataaatc
     1801 atttcttctg agactttgtc tttctggatt atatatttct tacactgcac aacggtttta
     1861 ctttgaccag ttgatagttt cttcgtgatc ttttctgtag cggtacaccg tttaccgtag
     1921 tgacaaatat cttcgagact caaaattcct gtatatgaca taaaataatc cacccactta
     1981 ctttaaatat ttttaacaag tttttcttgt tatttttcaa aaatagagaa ttgattttcg
     2041 ttcatgacaa acaaaaaaga gggaacgcat tcaaacatgc gttccctcta atttcagcgt
     2101 tcgtcatacg gcaaagagcc ccacggacat ttttataaca ttatgcttag gcatagattc
     2161 atcgtaatat cacgtatttt taaccttaac acagccaaaa aaagtggtat cacttttgaa
     2221 tatctgttat actaaatccg ttacgggtaa taccgtaaca ctgaaaggta aacgcattaa
     2281 ggggtataga cttaatgcca cctgtggacg tcgacgaagg ttggtagcct tccgagtctc
     2341 acagggcctt tctttttttt gtattttcaa ttgtccaaat ttatcgtcca aaagtgcaga
     2401 acgaaaatca attcatcaca agcatagcag acaattaaat ttaaaacaac taaatttata
     2461 caaaaaagtg taattgaata ttagcagcac tgcctgaaaa gaagtaatat caggcttttc
     2521 agcttgggta aataagtgaa ttacctctct tcatgaatta ttataactca aaaaaataca
     2581 tatgcaaaca ctagttccct gtttttttga atgaaaaata actaaaatat ctaacgatta
     2641 tgacattaaa acctacgttt gtgattgttt tcataaactt ttaatctaaa aactccggaa
     2701 taataactat ttatttgtaa catttgtaat aaaattaaaa ttgtaaaaaa gttctaattt
     2761 aaaattgatc atttttatag tgttcgcttt ttatcaacta ctctagccaa ttttccgtac
     2821 taactgttgg taattcgtta aatgtttctc tttgttcgtt tgcaagcttt gccgcaatag
     2881 ctttttcaat aaaccagttt tttacataca caaaaatcaa attctgcaaa ttatttactt
     2941 tctcagtctt ttgtttttga taagctttca gcaacgtcat atataatcct ttatccgcat
     3001 caattccgta agattcaagt tcttcataca caactaccgt cttctcaagt tcctcggctt
     3061 tatgttttgc tttgtgtatt gtggtgacgg tttgttgggc ttcttcgtaa gtgttagaaa
     3121 atgtttttat caagcttaat accttttctg gtacaaatgt gtttatgctg tcttctttca
     3181 tgatttcaac gaagttatct agcaaaactt gattttgtaa ttggtctgtt tcagtgtctt
     3241 ttaagtgtct tttagtgtct aaagatttat taatataata atcttgtgcg atttccgcat
     3301 ttccagtgtt atcaagggtt tcagctgttt tttctcgacg tgcgatttcc gcatttccag
     3361 tgttatcaag ggttttagca gtttcctctc gacgtgcgat ttccgcattc cgagaagagg
     3421 ttgatttatc aatagtttcg cttaaatcgt catcgaaaga attttgaata aaaagatcat
     3481 cggcacttat ttcaggttga agtaaatagt atttattttt ttcatttttt ttcgtttttg
     3541 ggttaaattc tccttttacg actgaaatca aattatatgt ttctaactcg tttaaaatgg
     3601 tttttacttt gccttggtag caatttaatt tgtccattag gtctgcttgt gtaaattcca
     3661 aatataatag accatcatcg tcgatccatt tttcatgtaa ggctctacga aattcatttt
     3721 taaaaagcat ataggctatt tttgagctgt ctttcatttt agagtattta gaattctgaa
     3781 ttaaaaaact aggaaattta taatattttt caatataaac attcgttgct ttatatcccc
     3841 aatttttaat tatcattttt tgagattgat tttctgtaga cattaaattt ctccttcttc
     3901 ttagaaactt gtatatattc aggattttca acaagtgaac tataccatgt attaagggtg
     3961 aataaaacac tttcagtatt taaggtataa gttatatatt aaaaaaataa gtataagtta
     4021 tactttaaat acaaataatg tataacttat actttaaata ataattttat aatgttgatt
     4081 taatcgcaaa tttacacgtt gtttattaag ttatacctta aatatttaat gtataactta
     4141 tactttaata ttcatttatc tagtataact tatactttaa ttgaataccg catgaaaaaa
     4201 aattattttg gtaatttttc aaactcttct tgtatttctt ctcttatgtg ttcttctttt
     4261 gctactccta gtctatgctg gatatatgag tctgctaatg tattaacaat tttttcgaaa
     4321 gaaattctcc ctttttcact ttgtaactcc gtcatatagt cttgtaaaat tgagagtttg
     4381 ctatttaatg ccggagagat ctttttagga actgatacct tgtttctttt atcaggaatt
     4441 gaaactggtc ggcctctttt tttcaatgta atatttcttg tgttactcaa tgcactattg
     4501 ctaaagttat attcaccgct tattttcaca tcttggcgtt tgttattggg taataatggc
     4561 tcaaattcac cattctccat cattttagga atatcaaaat ctttaggcat tacttatgca
     4621 actccttatt atttatttaa aaactcttgt ctagcatcta gttctgccaa aatatttata
     4681 aatacttggt gtgctttata atcccattga tcaaaatttc catttttgta ttttcttaaa
     4741 tgtataccaa ttttagaata ccttctcaat cttttttgat aatgaatgag atttttggat
     4801 accacggttc cgtatttttc aattgcttct tctaggattt cttcatcaaa atcatcattt
     4861 ggttctacca tacaaggtac aatatctata atttgcaaat caagatcgta tctatcgacc
     4921 atgaattttt gataatttac atatttgttg actccctcta aggattcttc agctgtttga
     4981 aatgaaatta tcgaatagtc tgaagctgcc atagcattat cggaaaattc gctaatggtt
     5041 ggtggtacat caataaatat ttcttgataa ttgttcttta ttggaagaag taattttttc
     5101 aagacagtaa cttggtctaa ttcattttca tgtttattag caaaagcgga gaatgatcga
     5161 aaactagtgt cacatgccag aaggtcaaga ttttcattta ccttaataat actacttgct
     5221 aattctccat ttgcgacacc atttgttata gtggttttga tatcggttat accgaaagtt
     5281 cttgctatga cttgtgttaa gttcgcttga gggtctaaat caataataag cgtttttcta
     5341 tttcttatca ttgcattgtc ataagcaagc atgctgacta tcttagtttt acctactcct
     5401 cctttaaaat tgccaactgt aaatgtagtc ccttgaaaag tatccatgta aatcctccca
     5461 atctttaatt gataattaaa gtataactta tatatcggaa atggtcaaca ttaaaagtat
     5521 aacttaaata ataatatcta aaaacgtttg gatatagcgg tttttaataa taaaagtata
     5581 agttatacca tatatattta aggtataact tatactttta ttattaaagg tataacttat
     5641 accttaaata ttgcttttcg aaaatagcta ttctagtcta tctgtttctt tacttttcgc
     5701 tgctgatttg aattgaattg atcatattca ttttctagta ttaataactt ttcgtataca
     5761 tcggtcatat tcatactaga agcaagactt agatcatata c
//
LOCUS       DACEQH010000101         5665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.106, whole genome shotgun sequence.
ACCESSION   DACEQH010000101 DACEQH010000000
VERSION     DACEQH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.106"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..194)
                     /locus_tag="IW833_002598"
     CDS             complement(<1..194)
                     /locus_tag="IW833_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308652.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFE"
     gene            complement(737..2887)
                     /locus_tag="IW833_002599"
                     /pseudo
     CDS             complement(737..2887)
                     /locus_tag="IW833_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3033..3608
                     /locus_tag="IW833_002600"
     CDS             3033..3608
                     /locus_tag="IW833_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1308653.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3822..4517
                     /gene="vanR"
                     /locus_tag="IW833_002601"
     CDS             3822..4517
                     /gene="vanR"
                     /locus_tag="IW833_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1308654.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4495..5649
                     /locus_tag="IW833_002602"
     CDS             4495..5649
                     /locus_tag="IW833_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1308655.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1770 a         1092 c         1073 g         1730 t
ORIGIN      
        1 tgttcaaaat ctaaaatcaa cacatcatgg ggagtgtgat aataaatatt ttcccttaat
       61 gtaccaacaa tggtaattcg gtcaattgac cattgcattt ttaagggggt gcacatagtt
      121 tgtgcattta accgcctgtt agaaatacgg ttaagttttt cccactcatt tacgattatg
      181 ccttttttac tcaaaaatag ctcctcctca acgaaaaaag agattcttgg aaagaatttc
      241 cagagttcac gtaactctaa aaatccacat ccaaaaatct ctagcttgaa gaaacctaaa
      301 aatattcgta taatgaactt gagttacgga actaggtgaa gtagtttccg taaaggtcga
      361 gagtgcgtca acactgctcg actatttttt tactcttttt tctcattttc tttgattata
      421 gcactcctct agaaaaaata aaaagacctc agggaatttc attccccgaa ccccttgagc
      481 ctcctcctgc cgaaatagaa tggcgctgcc agcatttatt tcgttcagct atcggctaca
      541 caatctaaca cgaccgttcc gctaccagtc gccttacagg ctccattttc gctcctctaa
      601 cgtgtttcac cgattgtgcc gataactaaa cgaaataaac gctaaaaccg ctcagaacgc
      661 aaaatatgcc attttagagc tattgtagaa aatatagttt gaaatgcaat tttcttcttt
      721 cagcttttta tttcccactt gtaaatcggt agatggtggc tccttttcca gttccataat
      781 gtggatttgc atctgcatgt agtgatgaaa cacctagctg cattctcata ccatctgacg
      841 aagatgttgt accgtcgccc caatagaaag gcaattgtaa tttatgatga aagtttacta
      901 atatggcttg ggctttattc atggcatctt catacatgcg ccattgagat acattggcta
      961 gttgcttata tgtaagtccg ggtgtggctt cggccatctt gctcaagcca atattcattc
     1021 ccattcctaa aagggcagcc atgataatga ttgtttcttc cttatctggt tttcgattat
     1081 tggaagcatg agtgaattgc tcatgaaatc ctgttatatg ggccacatcc atgagtaaat
     1141 cagttaattt tattcttggt agcatctgat aaaggcttgc actaaatttt tttgcttctt
     1201 ctggaacatc tttttctaag cgtgcaagtg atagctttcc tttttcaaga gaaaccccat
     1261 ctaacttatt ggaattggca gctaaccact ttaacctttc attaaagctg ctggttctct
     1321 ccgttatata atcttcgaat gataaactaa ctgataatct cgtattcccc ttcgattgat
     1381 tccatgtatc ttccgaaaac aaatattcct caaaatccct atattgtctg ctgccaacaa
     1441 tggaaacatc tcctgcccga acatgctccc gaagttctgt taaaacagcc atttcatagt
     1501 aatgacgatt aattgttgta ccatcatcct cgtataaatg tcttttccat cgttttgaaa
     1561 taaaatccac aggtgagtca tcaggcactt ttcgctttcc agattcgttc attcctcgga
     1621 taatctcaac agcttgtaaa agtggctcat ttgcctttgt agaatgaaat tccaatactc
     1681 ttaatagcgt tggcgtatat tttcttagtg aataaaaccg tttttgcagt aagtctaaat
     1741 aatcatagtc ggcaggacgt gcaagttcct gagcctcttc tactgaagag acaaaggtat
     1801 tccattcaat aaccgattct aaaaccttaa aaacgtctaa tttttcctct cttgctttaa
     1861 ttaatgcttg tccgatgttc gtaaagtgta taactttctc atttagcttt ttaccgtttt
     1921 gtttctggat ttcctcttga gccttacgac cttttgataa caaactaagt atttgcctat
     1981 catgaatttc aaacgcttta tccgttagct cctgagtaag ttgtaataaa tagatggtta
     2041 atatcgaata acgtttattt tcttgaaagt cacggaatgc atacggctcg tatcttgagc
     2101 ctaagcgaga cagctgcaac aggcggttac ggtgcaaatg actaatttgc actgtttcta
     2161 aatccattcc tcgtatgtat tcgagtcgtt ctattatttt tagaaaagtt tcgggtgaag
     2221 gatgacccgg tggctctttt aaccaaccca atatcgtttt attggattcg gatggatgct
     2281 gcgaggtaat aatcccttca agcttttctt tttgctcatt tgttagagat ttactaaccg
     2341 tattaaatag cttcttttca gccattgccc ttgcttccca caccattctt tcaagtgtag
     2401 tgatagcagg cagtataatt ttgttttttc ttagaaaatc tatgcattca tgcagtagat
     2461 gaatggcatc accattttcc aaagctaatt gatgaaggta cttaaatgtc attcgatatt
     2521 cactcagggt aaaagttaca aagtcgtatt cacttcgaat ttctttcaaa tgatcccaaa
     2581 gtgtattttc cctttgagga taatgatcaa gcgaggatgg actaacacca atctgtttcg
     2641 atatatattg tatgaccgaa tctgggatgc ttttgatatg agtgtatggc caaccgggat
     2701 accgaagaac agctaattga acagcaaatc ctaaacggtt ttcttccctc cttcgcttat
     2761 taactatttc taaatcccgt ttggaaaaag tgaagtaggt ccccagtatc cattcatctt
     2821 cagggatttg cataaaagcc tgtctctgtt ccggtgtaag caattctcta cctctcgcaa
     2881 ttttcattca gtatcattcc atttctgtat tttcaattta ttagttcaat tatatatcaa
     2941 tagagtgtac tctattgata caaatgtagt agactgataa aatcatagtt aagagcgtct
     3001 cataagactt gtctcaaaaa tgaggtgata ttttgcggaa aatcggttat attcgtgtca
     3061 gttcgactaa ccagaatcct tcaagacaat ttcagcagtt gaacgagatc ggaatggata
     3121 ttatatatga agagaaagtt tcaggagcaa caaaggatcg cgagcaactt caaaaagtgt
     3181 tagacgattt acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta
     3241 gtacacaaga tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat
     3301 cactaaaaga tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta
     3361 ctgtaatggc tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag
     3421 ggattgaatt ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa
     3481 atcacgcagg aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc
     3541 aaatttgtga aattactaat gtatctaggg cttcattata caggaaatta tcagaagtga
     3601 ataattagcc attctgtatt ccgctaatgg gcaatatttt taaagaagaa aaggaaacta
     3661 taaaatatta acagcctcct agcgatgccg aaaagccctt tgataaaaaa agaatcatca
     3721 tcttaagaaa ttcttagtca tttattatgt aaatgcttat aaattcggcc ctataatctg
     3781 ataaattatt aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta
     3841 ttgtggatga tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt
     3901 atacggtttt caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga
     3961 ttgaccttgc catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa
     4021 aaataaggga caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag
     4081 ataaaattac agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac
     4141 tggagttaat tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa
     4201 aggagcagaa cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg
     4261 agtgttatct gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa
     4321 tcctctgtga aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg
     4381 gcgacgaata tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg
     4441 aaaaaatgaa cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt
     4501 ataaaattga aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat
     4561 atcgttgcaa ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga
     4621 gggaaacttg gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg
     4681 gacgcgatga aattatatca atattccata cggaacaata tagatatctt tatttatgtg
     4741 gcgattgtca ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac
     4801 tttgacgaga taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag
     4861 ctttctgcgg aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa
     4921 aagcgagagc aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg
     4981 gcgcacgata ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag
     5041 gctccagaca tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg
     5101 tatcgactcg aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg
     5161 ataacgctaa caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa
     5221 ttttatcctc agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg
     5281 accgtgtccg gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc
     5341 gctgcataca gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg
     5401 gtgtcaatcg aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt
     5461 gaaaagttct ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga
     5521 ttggcgattg caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat
     5581 gataactata cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg
     5641 aggtcctaag agatgtatat aattt
//
LOCUS       DACEQH010000102         5472 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.107, whole genome shotgun sequence.
ACCESSION   DACEQH010000102 DACEQH010000000
VERSION     DACEQH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.107"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            30..704
                     /locus_tag="IW833_002603"
     CDS             30..704
                     /locus_tag="IW833_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1308656.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            709..2049
                     /locus_tag="IW833_002604"
     CDS             709..2049
                     /locus_tag="IW833_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1308657.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(2198..3184)
                     /locus_tag="IW833_002605"
     CDS             complement(2198..3184)
                     /locus_tag="IW833_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ1308658.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            3908..4939
                     /locus_tag="IW833_002606"
     CDS             3908..4939
                     /locus_tag="IW833_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1308659.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            5048..>5472
                     /locus_tag="IW833_002607"
     CDS             5048..>5472
                     /locus_tag="IW833_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1308660.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT         1800 a          965 c          953 g         1754 t
ORIGIN      
        1 taaagtgacc aaatcaatga ggtgggaaaa tgattaaact attagtagta gaagacgata
       61 aagtattatc caataatata aaagaaatac tcgctgcaat cggcgagata acacaagttt
      121 atgatggtag tgaaggactg ttcgagttgg aaagtggagt ttatgatctt gcaatcttgg
      181 atttgatgat gccggaaatg aatggatatg aagtgttagc aaaaatacgg aaagaaaaaa
      241 tacagactcc agtattgatt ttgactgcaa aagatagttt ggaagataag attgaaggat
      301 ttgaacgagg tgcagatgat tatctaacaa aacctttcta tcgagaagaa ctgatcatgc
      361 gcgtgaaagc attattgaaa cgctcgctag gtctctttga tgaaaatgtt ctgaagtata
      421 aggatatcag ttgtgattta aaaagcaatg aagtcttgta taaagaacaa gttttgccca
      481 tacaaggaaa agagtttgaa ttgctcgttt attttttaca aaataaggga atcattttga
      541 caaaagagca gatatttgac cggatctggg gatttgattc tgatacgacg attacagtta
      601 ttgaagttta tatgagcaac ttacgaaaac atctgcgaag tactggattg gataaagaaa
      661 tcaaaacctt acgtaatgtc ggttatatac tgcaaagggt gtgatgcaat gaatggagat
      721 attacacgaa agcaacgtag tcgctttttt gcgacaaatg tagcagcatt tgcttttatc
      781 ttttttcttt tgggattcat tactttacag atattaaacc aatctgcata cagagaaaca
      841 gatctgtcat tagagaaaat aaattttgat tctcgaacag tacaattaga gatacaaaga
      901 tatcagcaag gcgatccctt tttaaacccg actcaaacgg aacaaaacag aacaaacatg
      961 gaacagccat taatggaacc atcaaatgac cgttttaaca ctcaagtgat cttgtggtct
     1021 aaaaatggtg agatattgaa taaagcagct attggtgggc gtttcaacca aatcaaagaa
     1081 ttaaaaatgg atacgaaaaa tctgaatacc atccaaaata ttgaattaaa tgaagacagt
     1141 ggctcatctg ccctctcttt ccgttcgatt acgaaaaaag cggagacgaa tcaagatggc
     1201 gttgcctaca tccaaatttt agaaaataca aatcaaatca agaattcatt agcgactttt
     1261 cgcacgatta tgattttgtg tatgattgtt ttttggattc tctctattgg tatcagttat
     1321 tacttgtcta gtctaagcat gcgccctatt attctttctt ggagaagaca aaaagaattt
     1381 gttgagaatg cttctcatga attgcgcaca ccgttaacga ttattcaaaa tagtttggaa
     1441 catcttttta ccaagcctaa tcatactatt attgaagaat ccgaaagcat cgctcaagca
     1501 ttgtcagaga cgaggcgcct cactggattg acttcggatt tgctgacaat cgctcggaat
     1561 gattctgatc agcagctgct ttcaaagcaa atgatcaata cacaagaata tatcgaacaa
     1621 cttgttaaac catttcaaga gatggccatc atagatggaa aagagtttat tctccataac
     1681 gatgcaacat tagaagtacc aattgatgaa aaaaaaatcc atcaagtttt ggttattttg
     1741 ctggataatg cattaaaata tacgaatcca gaagataaga tcattttgga ttcatctgtt
     1801 aaccataaag aatggctgat tcacgtaaag aatactggcc cttctatcag tgaagaagat
     1861 caagcaagga tttttgatcg attttatcgg gaagatcaat ctcgggcgaa agagactggc
     1921 ggatacgggc ttggcttggc aattgcaaaa caggtaatcg aacagcatca tggacagatt
     1981 tctgtatcca attatcttcc acaaggagtc gattttaaag tccaattgcc gttgaagcct
     2041 aaaaaataat gcttatccct aaaaaagggc tgtgacaaaa atcttgtcac agcccttttt
     2101 tgttgcgaat aaacggtgtt ccagaagtag cttcttcgga aataagccga aatttcttta
     2161 agctgaccac ttctgtcaca acctctttag cttacgctta cgatttttta ttgcggtctt
     2221 caatgtgtac atcaccgctt gtgtcaatat ccaattcttc tcggctaaca tcttctgtca
     2281 ctttttcaac atcttcatgt tcttctttat gaatcccaac ttcttcacga ataactgtat
     2341 gtttgcttac atctacttgc tcttctttga ttgggatagt aatcgtgtca tcttccaatt
     2401 tttcgtctgt tccctgtgaa gattgatctg ttactggttt gcgttcgatg acgatttctt
     2461 cacgtttgac cggtacttct accgtttcag tatcgttgac cacatgtttg tggatgtcga
     2521 cttctccagt tgtcacatca tgtgtattta catctaattt ttcttctttt agttgaattg
     2581 tatcatcatc tagatttggt ttgcgatcga tgtcttcagt cttgcttgta cccattgtat
     2641 cagtctcgcc aggtacgtcc atcggatcag ttcctggtgt catattcaat ggatccgttg
     2701 tattttgaaa gcctgtcgtg ccacctacgt ccattggatc tgttccagtg ccagtttcta
     2761 atccagtccc cattgtgctt gctgggctaa cagtatcttg tgtatccatt gtatcttctg
     2821 ttgtttctgg ctgacgatag ccttttactg tgatgacaaa ttttccatca gaaatatctc
     2881 ctctatattg atataaagga tcattttctg gtgtagccgt ctcagaatca taggtatcaa
     2941 aagaaaaagt atcttttatt ttttcccata aagaacggtc ttcttcacca tccatccgat
     3001 tttcatctgt ttcaacatct ataccagaca atccgtcaaa tcctagtcta gatgctgctt
     3061 catcagtagt ataaataacg atatcatctc gttcataccc ttcgttacgc aaccgttcaa
     3121 ttactgataa tgcttcttcc ctcgtactat agcttccgat gaccgttaaa tttttctctt
     3181 ccatgtaaat tcctcctagc aaatagattt atgattggta tattgttact tataaattta
     3241 ttctatagga gacgattgtt ttctacaaat atttagattg gaatttgatt aaaacaattt
     3301 aattgaaatt tgaaaacatt tatgttaatg aaagtcatat tctaataaaa tgtcatttgt
     3361 ttctatatga atttttatga aaaatttcat ataatttcca ctcgttgtga tggtttaatt
     3421 tatccaatcc atcaataagc aaacaactta tataaaaaag gattaattct caatataatt
     3481 gctcctacca tagaataaat cttcatgtaa agtaatgaag taccaactat ttctaatact
     3541 tctttaagaa ttatcccaac aatatcgtta gacaaaccat tattgaataa atggagagcc
     3601 aatcgattct gaaaaaatgt tttctaattt ttcggaaata aaagaaagag cctcctgcca
     3661 ttagaacgaa ccaaatccat ttcattgctc atgaagtaac cacatagcta aaaagaggat
     3721 aaatcaatta aaagatcaaa agaatgactg cactaataaa ataactaaaa atgtatcttc
     3781 ttacttcata tcttttagct agtacttagt taaaaatttg cttcctaaga tcaagacaaa
     3841 ctgtctatct tcatttgatg aaatgcggta aaatatagaa aatgaacact attggaggga
     3901 atttgtaatg gacagcaaaa ctagaaaaca actgcaagaa aagttaaagc aacgtacaca
     3961 cttgctccct tccgtctttc aacagttttt agaagaaaat cgttcttccc tttcattagg
     4021 cagtcgctat gaatacgcaa aagattttca tctgtttact gattatttga gaaaagtatt
     4081 ttctgaagaa gtggatgatc agctgattat ccatttagaa aaacaagatt atcagaattt
     4141 cttagctaaa atctatcgac atactcgttc gttccaaact acagcgaacc aagaaacgga
     4201 gcaattgttt gaaaatagct attacgggat ttcaaggaag caatattcat taaatcattt
     4261 tttacgattt ttgtatcaaa aaggactgat tgaagaagag atttctcttt tgggtacaag
     4321 tttaccggaa acaaccaaag cagctccgcg atatatcgat gcagaacttt tcaaagattt
     4381 tgagcatttg tttatcccga ttgaaggctt tcaaacaagc agagaagcta gctttgaaga
     4441 aaagaataga ccgaggaacc ttgcaattac tgccattctt ctgtattgcg gattaaagat
     4501 ccatgaagta gtagaactta aacgtaaaga tgtatctttg accaagcaga ttcttaatct
     4561 gaatagaaaa gaaaacagac tgaataaagt accaatacca aaagaagcga tgccatttct
     4621 ggagaactat tttaagttaa cagcacaaaa tacggacgaa aattcttttg tttttcgttc
     4681 ctcacatgat cagcaaattt ctcctcggac tattcgccaa attttgagca aaattacagt
     4741 atataaaaac gtatcgccag aactatgcag aaaaacatat cgttattatg cagaactata
     4801 tttagatgat gctgacgcgg taaattattt atgtggtaat cgatacctct catcccattc
     4861 ttttcaagat atctgggaaa agatggttga tttttcttat cacgaattag cggcattcgt
     4921 tcgggtttct tccatataaa acaaatgtat gatctttttt tcacaataaa ctatgttatt
     4981 atataaagga ttatgttacg attatcttga atttgtatat tacttaacaa aagataatgg
     5041 agtgtgaatg atggagacca taacgtattc ccctttgaac ttatttacga attttcaaga
     5101 agctgcagct aacttttccg agaccccgat tatctttgat gagccacttt cagctttccc
     5161 agaattagga cttgaaacaa cttacaaaaa atgctgcgaa agtgtgttga aacgggctta
     5221 tcaattggct catcttggag tgaaatccgg agacaaagtt attatttata agagttccgc
     5281 ttttgacaca taccttctag ctgtttctgt gtcttacctt ggtgcagtgc ctgtcatgac
     5341 ttcttatcat ctaccaacaa caacaatgga agtatttatc gatcgtttag aagacccttt
     5401 cattttattt gatgatgaga caaaaaagcg agtgagagaa atttcaaatg gaacaaaatc
     5461 aaaacagatt cc
//
LOCUS       DACEQH010000103         5463 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.108, whole genome shotgun sequence.
ACCESSION   DACEQH010000103 DACEQH010000000
VERSION     DACEQH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.108"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            132..1106
                     /locus_tag="IW833_002608"
     CDS             132..1106
                     /locus_tag="IW833_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1308661.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1088..1549
                     /locus_tag="IW833_002609"
     CDS             1088..1549
                     /locus_tag="IW833_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1308662.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1599..2063
                     /locus_tag="IW833_002610"
     CDS             1599..2063
                     /locus_tag="IW833_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1308663.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2077..2826
                     /locus_tag="IW833_002611"
     CDS             2077..2826
                     /locus_tag="IW833_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1308664.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2826..3656
                     /locus_tag="IW833_002612"
     CDS             2826..3656
                     /locus_tag="IW833_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1308665.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3656..4303
                     /locus_tag="IW833_002613"
     CDS             3656..4303
                     /locus_tag="IW833_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1308666.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKINYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4401..5177)
                     /locus_tag="IW833_002614"
     CDS             complement(4401..5177)
                     /locus_tag="IW833_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1308667.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5259..5402
                     /locus_tag="IW833_002615"
                     /pseudo
     CDS             5259..5402
                     /locus_tag="IW833_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1873 a          738 c          972 g         1880 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat ttttttgaaa aatagtattg caaaataaag
       61 caatttcata gataataggt atcgtaaagt tatataactt tataatacct attagagtga
      121 gggataaaga aatgagacaa ctacacaaag aacagattga aacagcaatg gcagcgttaa
      181 aggaaaagac aattgatcat gtatatttta cagcatgtgg gggttcacaa gccgttttaa
      241 tggctggtca atatctattt gataaagaat taactgtacc ttgtcatgtt tatactgcta
      301 acgaatttaa ctatgctata ccaaaaggat tatctgaaaa ttctttagtt atcacttgtt
      361 cccattctgg cacaacacca gaaactgtag aagcaacaaa aaaagcaaga gaatttggag
      421 caatgactat ttgtttatct aatgaagaag gatctcctct atgggaagca gctgaatatc
      481 ctgttcacta tgattggggt aaagatgttg atgctagtga tttaaataaa ggaattcttt
      541 atggtttatt atttaatatt ttacttgttt tagatggctc taaaaaatgg gaaacttgct
      601 tgaatgaatt tgataagtta aatgatcttg ttattgctga aaagagcaaa tttgaaataa
      661 ctgctaaaga atggggtaaa aaccaaaaac gagataaaat tatttacaca attggtagcg
      721 gtattaatta cggagaaatt tactccacag cagcttgctg gttcatggaa atgcaatgga
      781 ttaattctaa tgctattcat tctggggaat ttttccatgg tccttttgaa atcactgatt
      841 atgatgtacc attcatctta gtgaaaagta ttggagatac aagattttta gatcaacggg
      901 tagaagactt cactaaaaaa ttcagtaata aattaatggt tttagaccaa gctgattttg
      961 acttagaatc tatagatagt gtagcgaaag aatatattgc agctgtactt agtggcgttg
     1021 ttattcgcca aatggtagaa gcgattgctt ttgaaagagg ccattcctta gatgttagac
     1081 gctatatgtg gcaaatgaaa tattaattaa taggaggttg caaaagatgg caaatcaatt
     1141 atttcacatt gtgattacaa cacattctac cctttgtgag ggttatttaa aggcaacaaa
     1201 attaatttta atgaatgacg ccacaggtat tagtgctgtt ccatttaaag aaaatactag
     1261 tccagatgat tttgaggaga gtatgaaaga aataattagt cagcatcatt tgcaacctct
     1321 aattatttta acggatttaa ttggaggaac acctaataat gtttctatta aacagttgaa
     1381 caacaataga attcaaatta ttgctgggat taatttacct cttttactgg agttattaat
     1441 ggcccagtca gctggtaaat ctatagaaga aatagatttt aaggaattac tgaataactc
     1501 tcaaaaaagt atggttcata ttaattcgct tttaaaagat gtcagttaag gtggcaattt
     1561 ctattctata aattttaaaa attgaaggag gaaatagaat gattaaatta gtgcgtgtag
     1621 accacagatt acttcatgga caagtaatat tttcttggac taaacagtat gatattaacc
     1681 atattattgt ggctgataac aaagttccag gagatccaat gtctgtgatg gctttgacaa
     1741 ttgctaaacc tgctgattgc aaattagaca ttgttaaaat tagtgatgtt aaagggatta
     1801 tggaaacatc cccaaatgat aattacatga ttttaattaa aggacctaag gaagcttttg
     1861 aattagttca aaatttacca gaaattaaag aaattaattt tggcggtgtg tctaagaaaa
     1921 gcgagtccaa acaatatgga aaggcagtct ttttaactcc tgaagaatta gaagccacaa
     1981 aaaaaattct tgatcaagaa gtgaaaatat ttgtgcaaca agtcccatct tcaagtattg
     2041 aaaatgtaga ttttagtaag taagggggga aaataaatgt tattaaaagc cattttatta
     2101 ggtttagttg gtgtggtagc tgttattgac tccaggttaa ttggtcgaca aaatattggt
     2161 agaccactaa ttttgagtac acttgccgga cttgtgctag gtgacttgca tacagggatt
     2221 gttcttgggg catctttaga attaatttct atgggattcg tctcaattgg ggcggctgga
     2281 cctcctaata tgcaacttgg tagtattatt gccactgctt ttgcaatttt aactaatagt
     2341 agtacagaaa cagccttaac aatagctatc ccagtggctg tagctggtga atttatgagt
     2401 attatcatgc gcatgataat tgctcaattt ggacatgctg cagataaagc cattgaaaat
     2461 aataaattta gaaaagctca aattattcat atttattggt catttatttt taatgctttt
     2521 gtttacttta ttccaatctt tttaacagta tattttggtg ctgacgttgt ggcagattta
     2581 gtagataaaa ttccagaaat gattacagac gccttgactg tctctggaaa tatgctttcc
     2641 gcattagggt ttgcaatgct attaagcaca atgttgagta aaaaattata tccttacttt
     2701 atttttggat tctttattgt tgcctattca ggtctttcac taattggcgt gacaattttt
     2761 gcagctctga ttgcctttgt aatggatcaa gttaaatatg gaaaaagaga agaggcgcat
     2821 ggttaatgga aaatagtaca gaaaaaaaac gtttaatcac aaaaaaagat cttcataaga
     2881 tgtgtcttcg ttcatggaca atcatgtgtt cgtggaatta tgaaagacaa atgcatatgg
     2941 gatttatgta tgggatggcg ccaattttaa ataaagttta taaaaatgat gaagagaaaa
     3001 aaagagaagc ttatactcgt catatggcat tttttaactg tacgcctcaa atgactccat
     3061 ttattatggg actttgtgct tctatggaag aacaaaatgc tcaagcagaa gaaggaaact
     3121 ttaatactga atctatctcc atgattaaaa ccagtttaat gggtcctttt gctggaattg
     3181 gtgattcatt tttccaaggt actttaagaa ttattacatt tgggattgga ttgtcttttg
     3241 ctaagcaagg tagtatttta ggaccgattt tagctgttgg cctttttgct ataccttcta
     3301 ttttagctgt tttttacggc ggtaaattag gatatgagtc tggcaataaa taccttacta
     3361 aattgtatca agaaggtctt atggatcgag taatgcaatt tgcttctatc attggtttgg
     3421 ctgtagtagg tggaatggtg gctagcatgg tgccaattgt caccccttta aaattcacta
     3481 cagggggaac agaacttgtt ttacaagact tacttgattc cattattcct ggattattac
     3541 cacttgtatg tactttggga atatataaat tgattaagaa aaatgttagt acaaatatgc
     3601 tcttattagg ttgtattttg gcagggtttg ttttaagtat aataggtatc ttataatgat
     3661 taaggctcta atttttgata tggacggagt tttaattgat agcgagatta tgtatcaaaa
     3721 gtgggtattt aattttttaa aacaagaaaa tttaagctat cctttgtatt cttatcaaaa
     3781 aaagattgga actacagcaa gtatttttga tgatatagaa aagtacaatg agggatgtaa
     3841 cagtttagaa ttgaagggaa aatttactaa atattggaaa tcgcaaaaga ttaattactt
     3901 ggatattttt cctaatatac taattgaaca gcttgaatgg ttaaaaaaga aaaaaataaa
     3961 aatggcaatt gcttcttcca gtccaatgga tactataaat gaagttgtta ccacttgtaa
     4021 aatagagtca tattttgatt gtttaattag cggaagagat ttgccagaaa gtaaacctaa
     4081 tcctactata ttccttaaag cttcagaaca gctcagaatt ccagttgaag aatgtctggt
     4141 gatagaagat tcttataatg gtataaaagc tggtaaaaga gcaaatatga aggttcttgc
     4201 aattaaagat aaacgttttt ctcaagatgt aagtctagca gatgatgtta tttacgatat
     4261 taaatatctg cgacaagaag tacaggtgaa atttggaata taaataagtt ttttgatgta
     4321 tttagagaat attagtaaaa ataaaaatca tgcccaaatc taatcaaaat tgattgcttt
     4381 gggtatgatt tcttttaata tcaatctatt actttaggat ctgaatcatt tttatgcacc
     4441 gtatctaaag tgatattttc tgtgacttca ataacataac taattctatc acaacgaact
     4501 gtggattgag aataatgaaa tggttgatta tttttatctt tgccaacctt ttctaaaata
     4561 aatagcggat ctcctactat acattgcaat aagtcggaaa gctcaggtgt tgcagataaa
     4621 ccgtttattt ccataataga gctcgtagct ttgacatcat agtcatcttc tagaagttta
     4681 tataatgaag tatattggct acctttttca aataaagttg gaaatttttc ttctgatgcg
     4741 aaaaaattat caattcccat aggggcaccg tcttcccaca ttaaacgttt aatattgtaa
     4801 actctatcag ttacattgat ttgtaattta ttagctaaaa actcatcagc ctttataatt
     4861 ttagaatgta aaacagtata tctaatttta tgtcctcggg tagaaagaga atcgctaaat
     4921 ccatgaactc cagctaattt taaactttct tttttttgag ttacaaaggt ccctttcccc
     4981 cttaatcgtt caactgttcc actttctgtt aaaagtttaa aagcttttcg aatggtaatc
     5041 ttacttactc cataaatttt acataattct tcttctgtag gtaattgctc tcctggaaga
     5101 tatttttttt cacgaatatc gttacgtata gaagcttcta cttgtagata taatggtcta
     5161 ttatcttgaa aattcatagt acactcccta aaaagttata cttcattata tcacaattta
     5221 ttaaaataaa agattaagat ataaatataa atgaactagt ctttgactaa ccaatgcccg
     5281 cttttgaaag aaaacaaatt cattctgttt taagtaaaaa tccttatgtt accactcatt
     5341 ctgaagggga tgagccttat cgctacttgg ttgtggaacc aagtgataag acaattttat
     5401 aaattcaata ttaattaaaa taaaaacaaa caaagtagat tattttactt tgtttgtttt
     5461 ttt
//
LOCUS       DACEQH010000104         5162 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.109, whole genome shotgun sequence.
ACCESSION   DACEQH010000104 DACEQH010000000
VERSION     DACEQH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.109"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            37..987
                     /locus_tag="IW833_002616"
     CDS             37..987
                     /locus_tag="IW833_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1308668.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            1505..1960
                     /locus_tag="IW833_002617"
     CDS             1505..1960
                     /locus_tag="IW833_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1308669.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2271..2627
                     /locus_tag="IW833_002618"
                     /pseudo
     CDS             2271..2627
                     /locus_tag="IW833_002618"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2640..3050
                     /locus_tag="IW833_002619"
                     /pseudo
     CDS             2640..3050
                     /locus_tag="IW833_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812298.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            3165..3329
                     /locus_tag="IW833_002620"
     CDS             3165..3329
                     /locus_tag="IW833_002620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308670.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            complement(3480..4459)
                     /locus_tag="IW833_002621"
                     /pseudo
     CDS             complement(3480..4459)
                     /locus_tag="IW833_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(4464..>5162)
                     /locus_tag="IW833_002622"
     CDS             complement(4464..>5162)
                     /locus_tag="IW833_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1308671.1"
                     /translation="EIIDELDISDMTARRDLDALEADGLLTRTHGGAQLLSSKKPLEK
                     THIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPGTTLEQLALELKGRKGYKIRVITN
                     SLPVFLILNDSETIDLLLLGGEYREITGAFVGSMASTNLKAIRFAKAFVSANAVTHNS
                     IATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYDFFNFYNLDQLDTIITDNQISPQH
                     LEEFSQYTTILKAD"
BASE COUNT         1715 a          852 c          926 g         1669 t
ORIGIN      
        1 ttatttctga gaaaaatata aagcaggagg attacaatgc tgaaaactta ttccttaaca
       61 aaagatacat tattagtttc gtcagaatca aatatcaaaa attcttctgt tgtttatgtt
      121 tatggtgacg aacgagaata tatcgaatct tctgaacata aatatgactt taaaataggc
      181 aatatactaa cgtttgatga tcgagttgca tatgacacga tgatagataa gaacgatgaa
      241 aaaagtttgc tcgtaagttt tttgtttccc gaggtacatg aaggtgggca tctagatacc
      301 ctgacgacat ctgttgtttt cttggtattt aaaaataagt tgataatttt tacaaaacaa
      361 aaattaaaat tcattcccga actcttatcc aatatggttt tacgtgatgt caatcatttc
      421 gtggaggaag tacttttaaa gatcatgcaa aaagatttct atatgatgct taacgacctt
      481 cgtggagtca aggaaaaaat tgatgattta gactcagaaa tatcaacgtc cggttcactc
      541 aaaccaacgt ttggtgattt attgactctt caaaaataca tgatagcttt gtcaacaact
      601 tataaagcta accataaagc gcttgatttc attaaaaaaa atttcacaac tattgacgat
      661 attgatttta gtactaaaaa aataattgat gatttatacg atacaagtga tacaatggat
      721 agaatcattt taagttacag ccaatatttg gacaaattgg aaaatatgat aaacaatatg
      781 atttcgtatc agctaaacat gattatgaaa accctaaccg agatatcaat tgttttgact
      841 attccagcaa ttatttttgg cttttgggga atcaatgtcc atgtcccgtt tgaaaagtct
      901 tcttatggtg ttttagccgt attgattatc tctgctatac taagtctaat ttgctggctt
      961 ttgatgcgac gcaaaacata tttgtaaagt atttgtgata ccctaatatg atttaaagca
     1021 gaatttcttt gttgactttt taaaaggctg attcctttca ataatttaat agcttcagcg
     1081 gctgctcaga agtcaataaa ttggactatt tttattcagt tacttaggca acatttgaga
     1141 tacgattgcg attataaaaa ttgatatacc aaccaatggc tgcttggaag actgacatat
     1201 tagcctcgat atcatcgaat aactaaatac agaaaaatac aataaatatt atcaaactag
     1261 ggactatact agaaaacatc agtacattcc ttaactcagg gtctataatt agaaaatcgc
     1321 ccctttgaaa actgattaaa acagccccca ttatctagtg actagataat gggggctgtt
     1381 tttttgttaa gtgatataat aaaccataaa agaagtttag aaatacggtc ctggtcatca
     1441 ataaataaac aatttaatta attatttatt taattattta atttcagaaa ggtatacatg
     1501 aatcatggtg caacaaattg tcctacccat caaagactcc agcgtcttaa agatggtaca
     1561 agatacactt ctcgatagtt tccgtgctgg tcgccgaaat tacaccgttt ttcaagttgg
     1621 taaggccacg ctactacgtg tgagtgatgt catgacatta aaaaaatcag atgtctataa
     1681 tccagacggc tctgtcaaaa atgcaacctt tattcatgat aaaaagaccg gtaaagcaaa
     1741 cacgttatat ttaaagcctg ttcagcaaga cttgttgcaa tatcatgatt ggctggccca
     1801 agaaaatatc aattctgagt ggttatttcc ctcgacagcc cattatgatc ttcatatcac
     1861 tgagaagcag tttcataagg tgatggcgca tgttggtgat ctcttaggaa tcaacttgga
     1921 agacaaagag aaaaaattgc gagtcagaaa accgttttga gatatgaaca gcatttgcct
     1981 tcacgcctta gcgattttaa ataatagcct tgaaagtgat tatggaaaag ccctctacgc
     2041 tcaatgcaaa attgacgtag aaactatttt tgatcgattg aaaggtgtat ttggtatgtg
     2101 aagaacccat gtcagaggca agaacgttgc acctactgac attggaatga tgcttatgag
     2161 catgaatcta accaaattat cccttgaggc tagaagaaaa gcggaagctt ttcaacacaa
     2221 agcagccaaa aataaaaaac gcgacgaagc aatcacattt atgattattc atcgcgtttt
     2281 ttatatttag agctagttat ttgctagacc cactatttac agatgaaacg tttatcacct
     2341 ttggttttgg gcatttagtg gaattagcag aaccaggtca ttatgacgaa aagtggcaaa
     2401 attggaaact tgaatctttg ccgatttttc ctgatcgata cgattttgaa gttgcaaaag
     2461 ataagggaaa gcagtttaaa attgttgcag aacttctcaa aaaggcaaat acaattattg
     2521 ttgcaacaga tagcgacaga gaaggtgaaa atatcgcctg gtcgattatc cataatgcaa
     2581 atgccttttc aaaagataaa acatttaaaa gactatggat caatagctta gaaaaagatg
     2641 tgatccgaag cggttttcaa aatttgcaac caggaatgaa ttactatccc ttttatcaag
     2701 aagcgcaaac acgccaaatt gccgattggt tgatcggcat gaacgcaagc cctttgtata
     2761 cgttaaattt acaacagaag ggcgtacaag gtacattttc actaggacgt gttcaaacgc
     2821 ccaccttata ccttattttt cagcgccagg aagccataga gaattttaaa aaagaacctt
     2881 ttttcgaggt ggaagctaag taaaacaagg gtcatttaag ggcgttctaa gccccacaca
     2941 gcgttttaaa acccaagagg agctttttag cttttgtttc ttctaaacaa gctaaaatag
     3001 gcaatcaaga ggggataatt gctgatgttc aaaccaaaga gaagaaaacg aatagtccga
     3061 gttcgttttc tttaagtagt ttgcaatcaa aagtcaatca gctttataaa gcgacagcga
     3121 gccaaacttt aaaagctatg caaggactgt atgaagcaaa attattgagt tatccaagaa
     3181 cagatacacc atttattaca gagaacgaat ttgcttattt aaaagcgaat tttggcaaat
     3241 atagcggttt tttaggactt gatcttgaaa tgttgttatc attgataatg atacgttcaa
     3301 acaacagcac ccaaattttg acgaattagt gaaactctat gaaaaagacg tagtaaaaca
     3361 tgcaacatcc tattctaatc agctggtaaa gcttaattag gttatataag cttgaaaata
     3421 aagggtttgt ccaatatgga caagcctttt tcttatgttt tgccacagat ttgccacact
     3481 tattgtgtaa gagtatcaag gataggtata atttgatact ttgttttttt agtgatgtgc
     3541 aaataaattt ctgttacacg actacctcta gaatgcccta cacggtcttg aattgcttct
     3601 aggggtattc cttactctgc taagaaacta atatgcgtat gacggaatat atgggtcgta
     3661 atatgtttat taaaactaag tttattatca atatcaattc ctgctcgttt tggattacct
     3721 tttcgattta attttccatt ttcaggtgag ttgaggaaat tgttaaggac actataatga
     3781 agaggtgttc cattatagtc gggtttatga cgacttttgg aagtaaaaat ccatccatta
     3841 tctttataat caggattgaa tctcatatgt tggtgattta actcaatttc ctctttaaga
     3901 atttctatag cccttttaga taattggaca tttcggtaac tttgtaagtt ttttacatta
     3961 ccttcatagc tattttctgc agctcctccg tgagtggact gactaatgat gatattttgc
     4021 ttttcaaaat attgataggc accaatattt tggagtttcg actctgtaag tcaacagggc
     4081 cattagttag aatacctatt gggatgcggt ttttatgtag agccagcagt agttgatgta
     4141 tctcaggaat catcgtaatc tgatcgagtt catattcata gtcagcttgg aagtctagag
     4201 cttctgtcct agaaatacta tcgatgccaa attcttccat agtcttctga cagcgaaaaa
     4261 ttctaagctg ctcaatactg atgagttttt tcgtgtattt aggaaaagca acatcagacc
     4321 aatatctgaa acgtttgtaa atgttatcta tttgttggat atcaatcgtt ggaaaacatt
     4381 tctcaacgct acgtttaaag gggagttgat ggtcataaag agtgtcatca aggtcaaaaa
     4441 caatattttt ataagtcata attctagtcc gcttttaaaa tagtagtgta ctggctaaat
     4501 tcctctaagt gttgagggct aatctggtta tctgtaatga tggtatcgag ttgatctaga
     4561 ttatagaagt taaagaaatc gtatcgatcg aatttagtac tgtctactaa taagaatttt
     4621 tctacagcat tgtttagggc aagttgttga atcacacctt ccttgtcact atatgtagca
     4681 atagaattat gggtaacagc atttgcacta acaaaagctt tggcaaatct aattgctttt
     4741 aaatttgtcg aagccattga acctacaaaa gctccagtta tttctctata ttcaccgcca
     4801 agaagcaata aatcaatggt ttcgctatca tttagaatca agaacacagg gagactattt
     4861 gtaatgacac gaattttata acctttacga cctttcaatt ctaatgccag ttgttcaagt
     4921 gtagttccag gtccaataaa aatagtatcg ccatctttga ttaaagagca ggctttttta
     4981 gcaatgtcaa ttttttcttt tgtatttaga cttttcttct cgatatgtgt cttttcaagt
     5041 ggctttttag aggaaagcaa ttgtgcacca ccatgagtac gtgttaaaag tccatcagct
     5101 tctagagcat caaggtctct acgggctgtc atatcggaaa tatctagttc atctattatt
     5161 tc
//
LOCUS       DACEQH010000105         4871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.111, whole genome shotgun sequence.
ACCESSION   DACEQH010000105 DACEQH010000000
VERSION     DACEQH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.111"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(75..1073)
                     /locus_tag="IW833_002623"
     CDS             complement(75..1073)
                     /locus_tag="IW833_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ1308672.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1148..1444)
                     /locus_tag="IW833_002624"
     CDS             complement(1148..1444)
                     /locus_tag="IW833_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1308673.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1437..1730)
                     /locus_tag="IW833_002625"
     CDS             complement(1437..1730)
                     /locus_tag="IW833_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308674.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1876..2427)
                     /locus_tag="IW833_002626"
     CDS             complement(1876..2427)
                     /locus_tag="IW833_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAQ1308675.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2601..2900)
                     /locus_tag="IW833_002627"
     CDS             complement(2601..2900)
                     /locus_tag="IW833_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAQ1308676.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2927..3193)
                     /locus_tag="IW833_002628"
     CDS             complement(2927..3193)
                     /locus_tag="IW833_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1308677.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            3480..4223
                     /locus_tag="IW833_002629"
     CDS             3480..4223
                     /locus_tag="IW833_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308678.1"
                     /translation="MVVSKLPRNEDYLTNYDGYVVTTEHFDLANPVTKVDDIPDYEMY
                     SQTIDSLNKRFGNRVLKGIKIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDD
                     EVKDMDPAIVIPQYFAQLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPIL
                     DQVIKNKLAFELNAKSAYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFD
                     DLFALLKANGVTELAIYQKGKRIMVPLPV"
     gene            complement(4260..4757)
                     /locus_tag="IW833_002630"
     CDS             complement(4260..4757)
                     /locus_tag="IW833_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1308679.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT         1452 a          929 c          708 g         1782 t
ORIGIN      
        1 taaaaaaact gattctaaga aaattaaata atttttctca aaatcagttt ttctttgata
       61 aataatattt tcaattattt aatcatttga aaattgttag tcattccaat acgttgagaa
      121 attcttccat ctgtataaac acgaaattgt tttccaccag ccatggtcgt gtttccatca
      181 tcatccgtta caatattaat tgaatagtag cttccatttt catcttgcat tagatctttc
      241 atatattcat ttgtgctaat taaattagta tttgatacat ttttatcagg agaatcatac
      301 aaccataaag ctttattatt tagcacagat tctttagcag cttcccaact tgaaattcct
      361 gaactacttg tagattgtgt cgttgtagtt gttgcttgtt gagtagattc ttctactact
      421 ggtgtttgag gagtttgtcc attcacaata tcactgaaac cttgttttaa ctgttgattt
      481 tgagtttcgt taaagtacag ataattattt tcattacctt gattttgttg cgttaaataa
      541 actttaggtt gtccattttg aaaaccgaaa aagtaaacat gctgccctaa atattctcca
      601 gtttctacat cagaataaac tgctactaaa ttgaaatctg catgaccagt tccatcttct
      661 gaccattgta cggtaactgg tgcttcattg attgccattt tccaatcgcc attaatcacg
      721 gcttgtggta aaggagtgcc atacagactt acattattct gaagattgta gcttttatat
      781 tcttgaccta atgtctttcc ccattgtgtg acaaaagttt ctaatttgga agctttattc
      841 gtatcccaca aagtattctt aacctcttca gaagaagatt cagtgattga acttgcactt
      901 gtttccacag attgcttcat ttctgttgta gtttgttcag ttgattgcgt tttacttttt
      961 ttagtctcaa ttgttgtatc agtttctata gtcgctttag tcctcgtatt ctcagatgta
     1021 caaccagtga ataaaattcc cattcctatt agtaataaca gccatttttt cattatattt
     1081 ccccctttca atttctccct cacgtactta ttctatcaga caattaattg atctaaatgg
     1141 taatctttta atcgattctt tcccaataat gtcgatagcc cgtatggcag cggtctcttc
     1201 cttgaggact ctcagatgga ttaaaactag tagaaatttc ccttccacat cctctacagc
     1261 gataagttct cattattttt gggctattag tattattttt cttagttaga gctatgagtc
     1321 caactatgat taccccaact gtcttaatta cttttccttt atctatattc ggatgcgcaa
     1381 gcatatattt atataaccaa tatccaccgt aaattaaacc acctaataca atcagcttag
     1441 ccatgtactc gtctcctttc tttttgttta attacttttt gaatccatct tgcagagaaa
     1501 ccataaactt ctgtcaattg acggatatct acagtttttt cctgttctat catgttttcg
     1561 attctttttc taactaactc acgatcgtac attttcattg gaaaagaaat ctgttgccct
     1621 cgaaaagcaa gataaaattg atagagcaat tctgaatcca gttccatttc ctcaaaaaag
     1681 tcattcatac ttttataaga gttactcaaa caagtttcat caatctccat ctacacaccc
     1741 cctgttattt tttacataat gaaaatagca tattactggg tacattgata attcatataa
     1801 catgtatcat cacatgtatt tttaaagtca aaaagcatgt aaaactactt ataagtaaca
     1861 tgagaaaata aaatgttatt tttcttgaaa taataattga ctatctgcca attgccattc
     1921 attacgaaca tgaatactta gtgggtggga taacttacct tctgcattta tcaatacttt
     1981 ggtcatcttc ttttgtttcg cactcaattg atcgtaaata gtctttgctt gttcataagc
     2041 cacttgactc gtcgctgtaa tcgaaccaca agcaaaaata atggtattga ttttcttttc
     2101 tgtaaaaaca gtcgttaata tttccaaaga ttgttcatca tttcctcgaa gaaatacccg
     2161 aggacttttc gcttttgtgt acgaaaaaag attcacagca ataaaaccag taaatcctaa
     2221 ttgtcggata ttcttttcga ttaataacat actcaaatca ttcgtaaatg gttctgtatc
     2281 tgttggacga atagtaatga ctactgctaa tttatcttcc gcttcttttc ctttgatcgc
     2341 ccattgtcgt ttgaaaacat agcgatgggt atgatgagga tctcgccaaa cttctgtttg
     2401 tgtggtcact aattcttttt caatcatctc tttttctcct atgctatttt ttgttcaagc
     2461 acttcaatta ccattgccgt tataagtgtc aatactagtt gaaaatattt catattcttt
     2521 ctcctttttt cttcagaatt tgatgaatcg cggtaaggat atcttctaaa catttcgcaa
     2581 tcattctcaa ataatcgatc ttattcatcc gttcctccaa tctgactcca aaccaatcgt
     2641 ccttctacca gtaacccacg aacaaattct cgctggctgg taatatctgt aatgaaataa
     2701 actacatagt cttttccatc ttctggtgcg caataaatat aaagatgtgt catcactaaa
     2761 cgtggtagat gaacatcttc aggaagcata cccatttcaa tttcctcttt ttccatcgta
     2821 acggattcat agccccactc ttcagaagca gtgaccagct gtgcacactt ttccagtact
     2881 tcgatagccg tttctaacaa gtcctttcgt tcctttcttt tagatactag tattcttctg
     2941 gtaataacat cgtttgatac tcacccgaat caatggccca tattttttga gacactgatt
     3001 tcaaacgaag cttaggtgaa atcagatact cttttctttc ttccggttct tcttgacgat
     3061 gaaccaactt ttgatttcct ttttccgtag aaaattcaaa aatttgaaga tagtctaaag
     3121 gaaattgctt ttgaacgttt tgatcgatta gattccaaca taaagcttgt aattccaccg
     3181 gcaaactgtc cattactccc tttgtgacat aacgttatcc tttattttga aacatttctt
     3241 ttccctcctt tgccataaaa atagcccaat cttctattaa agaagattgg gcttcatgga
     3301 tataatatat atttgtaaat aaagttaatt cagttataat tatcaaaact acacaattat
     3361 taaagttttt catatctctt tgtttagtaa gtttttctaa atcttaaccc gtggtgctag
     3421 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
     3481 tagtagtctc aaaactccca agaaatgagg actatttaac aaactacgat ggttacgtcg
     3541 tgacaactga acattttgac ttagccaatc ctgttactaa agtcgatgat atccccgatt
     3601 atgaaatgta cagtcaaaca atcgatagct taaataagcg ttttggcaat cgtgttttaa
     3661 aaggcatcaa aatcggttac atcgcttctg aaaaggatcg gatcatcgac tatttagccg
     3721 ataaagatta cgatttaaaa ttactcagtg tgcatcataa tgggcaattt gattatttag
     3781 atgacgaagt caaagatatg gatcctgcta ttgtgattcc tcagtatttt gcacaacttt
     3841 cagaagcact agttgttata gaagcagatg tctttgctca ttttgactat ggttttcggg
     3901 tatttggtct atccgttgct gaatttaaac aatatgaagc gcagttttta ccgattctag
     3961 atcaagtaat caaaaataaa ttggcattcg aattaaatgc taagagcgct tacttgtatg
     4021 acaatttagc gttatatgaa tatgtcatcg atttatatct ttcacgtggt ggtaccttgt
     4081 ttagtgttgg atcagatggg cattaccttg aacatttccg tttccatttc gatgatcttt
     4141 ttgctctgtt aaaagcaaat ggtgtgacgg aattagcgat ttatcaaaaa ggaaagcgaa
     4201 ttatggttcc attaccagtc taaaaaaacg tcctcagcat caaaagtgag gacgtttttt
     4261 tagatttctt tttctaaaat cggacaaata cgatcttgtt ttcctaaagg aaaatgtttg
     4321 atcccgattt ttttaaatcc atgacgttca taaaaacgaa tacctgcatg atttttttca
     4381 taaacagtca aacgtaaata acgtttttgt ttcgttttag cataacgatt cgcttcatcg
     4441 agtaaccgtt gccctaaccc tttgttttga taatctttga gcagatagag ccgttgaatc
     4501 tctatagcat ctttttcatc attgactttc atataaccaa tgagtatttg atgatcctta
     4561 ataccaataa atttactggt tgactgtgcc aattctactg ccaatatttc agttgtcaat
     4621 tgtgtctgta aatatccttg catatcaagt ggatctgcgc catcgataaa cgattcataa
     4681 aaactgacaa cacttacaac ttgtaaagca tctagatcat cataatctaa atacgctagt
     4741 gtgatttgat tgttcatcta actcctcctt ttgacatagt atactataaa tcacccgtgg
     4801 tgctagttaa ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc
     4861 tcgacttaat a
//
LOCUS       DACEQH010000106         4824 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.113, whole genome shotgun sequence.
ACCESSION   DACEQH010000106 DACEQH010000000
VERSION     DACEQH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.113"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            130..1275
                     /gene="tgt"
                     /locus_tag="IW833_002631"
     CDS             130..1275
                     /gene="tgt"
                     /locus_tag="IW833_002631"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ1308680.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1372..1752
                     /gene="yajC"
                     /locus_tag="IW833_002632"
     CDS             1372..1752
                     /gene="yajC"
                     /locus_tag="IW833_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ1308681.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKKEK"
     gene            2057..4654
                     /gene="adhE"
                     /locus_tag="IW833_002633"
                     /gene_synonym="adhC"
     CDS             2057..4654
                     /gene="adhE"
                     /locus_tag="IW833_002633"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ1308682.1"
                     /translation="MAKVMEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     AQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(4562..>4824)
                     /locus_tag="IW833_002634"
     CDS             complement(4562..>4824)
                     /locus_tag="IW833_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAQ1308683.1"
                     /translation="PLFFCSKIRNEVDKKLFQARVTSLEQLLSTVWNLEKQRFPSSFS
                     SIFLSFFYAFFCLSVVSINDDLFQFRDQWFFRISCRTLVFVS"
BASE COUNT         1688 a          854 c         1026 g         1256 t
ORIGIN      
        1 ataaaaaaag gataaaaaaa agaatattaa ataaaataga aggaagccga gggaacttgc
       61 aaaaagcact tgaaacgatt atgatggtgg gagttgtcaa gatattttat atagaaagta
      121 gggaaatgaa tgacgcaacc tgccattcgc tatcgtttaa tcaagaaaga aaaacatact
      181 ggcgcgcgtt taggggaatt gatcacgcca cacggaactt tccccacacc aatgttcatg
      241 ccagtaggaa cattagctac agtaaaaaca atgtccccag aagagttgaa agaaatgggt
      301 gctggcgtta ttttaagtaa tacatatcat ttatggctac gccctggtga agacttagta
      361 gaagaagctg gcggactgca taaatttatg aactgggatc aaccgatttt aacagattca
      421 ggcggctttc aagttttttc attaagtgat atgcggaata tcgaagaaga aggcgtgcat
      481 ttcaaaaatc acttgaacgg ttcgcgtatg tttttatcac cagaaaaagc aatcaacatc
      541 caaaataaat tgggctctga tatcatgatg agttttgacg aatgtccacc attcgatgaa
      601 agttacgact acgtgaaaaa atcagtagaa cgtacgtctc gatgggcaga acgaggattg
      661 aaagctcatg ctaacccaga ccgtcaagga ttatttggaa tcatccaagg agcaggattt
      721 gaagatcttc gtcgtcaaag cgcgaaagat ctagtcggga tggatttccc tggttactca
      781 atcggcgggt tatctgtcgg ggaacctaaa tcagaaatga atcgtgtatt agaattcact
      841 acaccgttga tccccgaaaa caaaccacgt tacttgatgg gtgtcggtgc tgctgattca
      901 ttgatcgatg gtgttatccg tggagtagac atgtttgact gtgtcttgcc tactcgaatc
      961 gctcgaaatg gcacatgtat gacttctcaa ggtcgtctag tagtgaaaaa tgcgaagtat
     1021 gctcatgatt tccgtccaat tgatgagaaa tgtgattgtt atacatgtaa aaactataca
     1081 cgtgcctata tccgccattt gatcaaatgt gatgaaacat ttgggattcg tttgacttct
     1141 taccacaatt tatacttctt gttgaatcta atgaaacaag tacgtcaagc aatcatggat
     1201 gataatttgc ttgaatttag agaagccttt tttgaagaat acgggttcaa taaagaaaac
     1261 gcaaaaagct tctaaaggaa aatcttttaa aaattaagta aaaaactagt gttagcagca
     1321 tttttctgct aaagtgtttt ttgtagatac agaattagtg aggtgaaaaa aatgggaaca
     1381 ttaccaatgc ttgtgatgtt tatcgcgcta ttagcaatgt ggttttttat gtcacgttcg
     1441 caaaagaaac aacagcaaga acgtcaaaac ttgttagata gcatgaaacc aggtgattca
     1501 gtcgtaacga tcggcggatt acatggagtt gtttcagaaa tcgacaatga caaacggaca
     1561 gttgttttag actgcgaagg gatcttcctt gagtacgatc gtgcagcaat caaaacggtc
     1621 aaaccaggga ctgtcgtaac aaatgatgca acaactactg ttgaaacaac agaagaacca
     1681 gctgagacag ttgaagtaaa aactgaaaca acagaaacgc tggaagagac agaagagaag
     1741 aaagaaaaat aagtatcaaa gcttatttaa agacgaggta gggcagaaag atgtcctacc
     1801 tcatttttta ttcaaaaaag aaagagataa gagtatagga tatattgctc aatattgaat
     1861 aatcgggtgt gtcatataaa taataataaa taactttata tatttttcta tacaaacgcc
     1921 tataaataaa gcaactttat atttaaaaat aactgtgaaa aaaatcacaa aaaagtgttt
     1981 acatagaaaa ataaatattg tatgatatac atgtgaatga tgtaacaaat aaccatgacc
     2041 cattaggagg ataatgatgg ctaaagtaat ggaaaaagaa aaaacgaaga caattgacgt
     2101 acaagcaatg attgatgatt tggcagaaaa agcaaatgca gcattgaaag aaatggaaag
     2161 ctttgatcaa gaaaaagtgg accatatcgt gcatgaaatg gcaatggcag ctttagatca
     2221 acatatgcca ttagcgaaga tggctgtaga ggaaacaggc cgcggtgttt acgaagataa
     2281 agcaatcaaa aatatgtatg cgtccgaata tatttggcat aatatcaaac atgataaaac
     2341 agttggtgta atcaatgaag atgcccaaaa aggattgatt gaaattgctg aaccagtcgg
     2401 tgtggtttgc ggagtaactc caacaacgaa tccgacttcc acaacgattt tcaaatcaat
     2461 gatcgcgttg aaaacaagaa atccaatcgt ttttgctttt catccaagtg cccaaaaatc
     2521 ttctgcagaa gcagcacgag tagtacgtga tgctgctatc gctgccggag ctcctgaaaa
     2581 ttgtatccaa tggatcgaac atccttcgat cgaagcgact tctatgctaa tgaaccatcc
     2641 aggtatcgcg atcgtcttag cgacaggtgg agctggaatg gtcaagtctg cttactctac
     2701 aggaaaacca gctttaggtg taggtccagg taacgtacca gcttatattg aaaaaacagc
     2761 aaaaattaaa cgtgcagtga acgatttgat cgtttcgaaa acatttgata atggaatgat
     2821 ttgtgcttct gaacaagctg tgatcgtgga taaagaaatc tatgcagcag taaaagcaga
     2881 attccaagca caccaagttt atatcgtaaa accagatgaa ttgcaaaaat tagaagatgc
     2941 agtgatgaac gaaggaaaat atgcggtcaa tccatcaatc gttggacatt ctgcgatgga
     3001 aatcgcaaaa ttggcaggaa tcagtgtacc aaaaggaacg aaaatgctga ttgctgaatt
     3061 ggaaggcgta ggcccagatt atccgctatc aagagaaaaa ctttctccag ttcttgccat
     3121 gatcaaagct aacaacacgg accatgcgtt tgatttgtgt gaaggtatgt tgaatcttgg
     3181 aggattagga catacagcag ttatccattc agaaaacgaa gagttgcatg tcaaattcgg
     3241 tcttcgaatg aaagcttgtc gtattttagt gaatacacca tctgcagaag gcggcatcgg
     3301 tgatatctat aacgaaatga ttccgtcatt gactttggga tgcggttctt atggtaagaa
     3361 ctctgtttct cgtaacgtct ctgctgtaaa cttaatcaat gtcaaaactg tagcgaaacg
     3421 gagaaataac atgcaatggt tcaaattacc gccaaaaatc ttctttgaaa agaactctct
     3481 attgtactta gagaaaatgg aaaacgttga acgtgtcatg atcgtttgtg accctggtat
     3541 ggtccaattc ggttactgcg atacagttcg tgaagtattg gcacgacgta aaaatgatgt
     3601 gaagatcgaa gtcttctcag atgttgaacc gaacccatcg acaaataccg tatacgcagg
     3661 aacgaaactg atggcagact tcaagccaga tacagtaatc gctcttggag gtggttctgc
     3721 aatggacgca gctaagggaa tgtggatgtt ctatgaacac ccagacacat ctttctttgg
     3781 tgcaaaacaa aaattcttgg atatccgtaa acggacatat aagatcgaca aaccagaaaa
     3841 aacacaattc gtatgtatcc cgacaacatc agggactggt tctgaagtaa cgccatttgc
     3901 ggtcatcact gacagtgaaa cacatgtgaa atatccgtta gcagattatg cattgacgcc
     3961 agatgtcgca atcgtcgatc ctcaattcgt catgtcagtt ccagcatctg tcacagcaga
     4021 taccgggatg gatgtgttaa cacatgcaat cgaatcctat gtttcagtca tggcttcaga
     4081 ttatacacgt ggattgagcc tacaagcaat caagttagtc tttgactatt tagaaaaatc
     4141 agtaaaaaca cctgatatgg aatcacgtga aaaaatgcac aacgcttcta caatggctgg
     4201 tatggcgttt gctaacgcat tcctaggtat ttgtcactca gtcgcacata aaatcggcgg
     4261 ggaatacggg atccctcatg gacggacaaa tgcgattctt ttaccgcata ttatccgcta
     4321 taatgcaaaa gatccacaaa aacatgcgat gttccctaaa tatgactatt tccgtgcaga
     4381 tacagattac gcagatattg caaaattctt aggtctgaaa ggcgaaacaa cagaggaatt
     4441 agtcgaagct ttagcaacgg ctgtttatga attaggaaaa tctgtcggca tcaacatgag
     4501 tctaaaagct caaggagtca cacaagaaac actagataca accgtagacc gtatggctga
     4561 attagcttac gaagaccaat gtacgacagc taatccgaaa gaaccattga tctctgaatt
     4621 gaaacagatc atcattgatg cttacaacgg ataagcaaaa gaaagcgtag aaaaaagata
     4681 agaaaataga tgaaaaagaa cttggaaaac gttgtttctc caagttccag actgtagaca
     4741 aaagctgttc caaactcgta actcgagctt ggaacagctt tttatctact tcgtttcgaa
     4801 tttttgaaca aaaaaataaa ggct
//
LOCUS       DACEQH010000107         4784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.114, whole genome shotgun sequence.
ACCESSION   DACEQH010000107 DACEQH010000000
VERSION     DACEQH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.114"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            544..1107
                     /locus_tag="IW833_002635"
     CDS             544..1107
                     /locus_tag="IW833_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1308684.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="IW833_002636"
     CDS             complement(1386..1673)
                     /locus_tag="IW833_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1308685.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="IW833_002637"
     CDS             complement(1663..1992)
                     /locus_tag="IW833_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1308686.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="IW833_002638"
     CDS             2297..2626
                     /locus_tag="IW833_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1308687.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IW833_002639"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IW833_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1308688.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /locus_tag="IW833_002640"
     CDS             complement(3551..4462)
                     /locus_tag="IW833_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ1308689.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1564 a          824 c          722 g         1674 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ataggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca
     4741 gcgcacgcag ataatgaccg caatatcgct ctgccttaca tctt
//
LOCUS       DACEQH010000108         4430 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.115, whole genome shotgun sequence.
ACCESSION   DACEQH010000108 DACEQH010000000
VERSION     DACEQH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.115"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(543..2048)
                     /locus_tag="IW833_002641"
     CDS             complement(543..2048)
                     /locus_tag="IW833_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1308690.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IW833_002642"
     CDS             complement(2045..2731)
                     /locus_tag="IW833_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1308691.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IW833_002643"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IW833_002643"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ1308692.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1238 a          919 c          729 g         1544 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat
//
LOCUS       DACEQH010000109         4374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.116, whole genome shotgun sequence.
ACCESSION   DACEQH010000109 DACEQH010000000
VERSION     DACEQH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.116"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(83..1051)
                     /locus_tag="IW833_002644"
     CDS             complement(83..1051)
                     /locus_tag="IW833_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1308693.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1498..2904)
                     /locus_tag="IW833_002645"
     CDS             complement(1498..2904)
                     /locus_tag="IW833_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ1308694.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3126..3488)
                     /locus_tag="IW833_002646"
     CDS             complement(3126..3488)
                     /locus_tag="IW833_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308695.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3492..3800)
                     /locus_tag="IW833_002647"
     CDS             complement(3492..3800)
                     /locus_tag="IW833_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308696.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3809..4228)
                     /locus_tag="IW833_002648"
     CDS             complement(3809..4228)
                     /locus_tag="IW833_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308697.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4221..4361)
                     /locus_tag="IW833_002649"
     CDS             complement(4221..4361)
                     /locus_tag="IW833_002649"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308698.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1234 a          994 c          619 g         1527 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aaattttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactatttt tttactcttt
     1261 tttcagttca tttgtttgct aactcaataa tagcacttct ctttagctat gcaaatatcc
     1321 aaaaaataaa accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaggttgg
     1381 ccaaaaaatg gactggccat tttttagcaa atgtcagctt aataggctag tggtttagcc
     1441 gtcaactcct tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta
     1501 ccactcgatc tcatcttcag aacgctctaa tttacgctgt gagacgtttt gacgataaaa
     1561 taaggttttc tcctgctcta acacttctaa ttcctcagaa agccccatat ccttttctgc
     1621 aagaccaaaa cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc
     1681 taaacgctgg cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc
     1741 tgctaattgt tttctaccag acaaatgacg ctcttcttcc aatgtagcta catactcatt
     1801 taattctgta ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct
     1861 cttggcattt gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa
     1921 atgatacgaa ccatcaggaa gcctcttgaa actttctaaa ggaaccatat ctttttggaa
     1981 aatgaccgcc atctcttttt ttacattcag catatttttc ttctcttctt caatcttcgc
     2041 tctctcagaa gccatcagag ccttatcacg ctcgttttct tctctttgcc tagaaatata
     2101 cctcaactca tcagaaacgc tctcacgctc ttttacagcc gtttggcgca attcagaaat
     2161 ttcactcatg atttctttgt attcgtgaat atctttaatt ttgttggtac cgacaatatc
     2221 ccttttccaa ccgtaatcat tcattaatcg ttctatagag tcaacttctt gatttctaaa
     2281 attattgaaa agcactcgac cattatcttt atcagcgtgt atcccttgtt gaaccagtgc
     2341 tttattaaag ctcggctgct tttgaacacc ccgtttataa ccttcagcaa ccggcacaat
     2401 attcaagtgc aggtgcggac ttatttcgtc attatgaata acagcattat aaacagccaa
     2461 attagggttt ctctcttgaa actccttgta atactgaacc aaaattttat tggcaatcac
     2521 tcgattttgt tcagcttgtt gtttcgatat gccagtagaa gaaccatctg ctcgatttgt
     2581 ctggaaatca tctaaaatgc ctatttgcac aataaattct cgttgaggtt ccaacttttt
     2641 atccttgcgc actttcgcta aatagtcctc gatttttcta tcggctcttt tttgtttcgc
     2701 attatatttt tctaccactt cgccaaacaa ttcctcatat ttatctgcta gattcgtctg
     2761 ctctaaataa atattttcat ccgatttcgt gaagtcaata tgcttatgcc aatcattggt
     2821 tttctggcga tcagttaact cacgattatt atgagaaata cttgtattct tcgttccttt
     2881 tttaaatgaa atactaaacc ccaagttaaa accctccttc gcgctgtcgc taataaaaag
     2941 attgtaaacc tttctgttac tctttgtcaa agagtaacat tttcctaatt gtgacatggc
     3001 tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca caagtgacaa cgaaaaaagg
     3061 agccgaaagc gtgtcacgct ctcaactcct tttacgcttt attttgcttc acgcaaaata
     3121 atcgcttatc gtaaattcac gtcattaaat attgacgtaa atttactctc actcatctga
     3181 cttttcttat tcggtatcac tccgaataag ccgtcagatt catcgggata atctcgttcg
     3241 cttggcgctg atttttcatc agcaagccac tcattatcca ctgatttttc gtgttgctga
     3301 tcgtcagaaa tcgacgatag ccaccgctca aaatcagcaa aagtttcaaa cccaaaacgc
     3361 ttgcgaagag ccttgcccat tgcagcattt tctttctcaa tcaagcgttt tttctgttgg
     3421 cgaagtttcg cttgttcttt ttttaatttt tcttcttgcg ctttaatatc tgctaaagtc
     3481 attcccataa actacatctc cttttctttg gaattaataa cggaaatttg gtttaactgc
     3541 ataacatgac tcattaattt caaccaaaat tctttttctg attcatcagt tgtttgcgct
     3601 aaatgcttat gaacaaattc attagtcaaa actgaagatt ttgctagatc agcattgata
     3661 accgagttga gaacatccat cattcgttca agcatttcac tataattatc ttcatccggc
     3721 aaattcaaat aatcagctaa ccgttcatac atcaattcaa ttctatcgac tgtttcttct
     3781 ctcaaatcaa ttttatccat gtcacacctc acttccagaa ttgccaccat tttcgatttt
     3841 ttaattcttg attttctagt aacaactgat cgtttttttg ttgctgaagc aaagtcaatt
     3901 tttgttggtc acgaatcatt tccgttaatt cagaaatatg cctatctttt tccgctaatt
     3961 gttcacgcaa aaattcattt tctttttcta acgggaaatc attgcctgca tcattgaccg
     4021 gtaaatcacc agtcaattga ccaccttttg accgataaat tgaccgcaca acaccaatca
     4081 attccttgtc taacatgata accccattat ctttataccg agcaaggtca taacctgcat
     4141 tgaccagttg attgcgcact gtatttttag atatacccaa ctcatcagca aggtttttta
     4201 tggtcatttt ttctggcaaa tcagtcatta tcttctccgt tactttcagc taccccataa
     4261 acatatttaa cagcatgctt agggataatg attggggaat caattttatt atttttcaaa
     4321 atcaactcat actgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DACEQH010000110         4221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.118, whole genome shotgun sequence.
ACCESSION   DACEQH010000110 DACEQH010000000
VERSION     DACEQH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.118"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..342
                     /locus_tag="IW833_002650"
     CDS             <1..342
                     /locus_tag="IW833_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308699.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
     gene            423..1043
                     /locus_tag="IW833_002651"
     CDS             423..1043
                     /locus_tag="IW833_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308700.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     EIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQIS"
     gene            complement(1373..2599)
                     /locus_tag="IW833_002652"
     CDS             complement(1373..2599)
                     /locus_tag="IW833_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1308701.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2671..2829)
                     /locus_tag="IW833_002653"
     CDS             complement(2671..2829)
                     /locus_tag="IW833_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1308702.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            3517..>4221
                     /locus_tag="IW833_002654"
     CDS             3517..>4221
                     /locus_tag="IW833_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1308703.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFI"
BASE COUNT         1448 a          590 c          689 g         1494 t
ORIGIN      
        1 aatataaaaa ctagtacgaa attaatttgt aaattattta agcttgataa aataagcaat
       61 atcgatgatg taaagtccaa gatattaaca agaaaagtaa gcgaaccagt aaagctaacg
      121 gcacaagata aaaaaaatgg aattgttctt tatggtgatg atgcaaagta taactataat
      181 tgggatacct cctcagaagc tgaggtatta agtattccta ctaccgatac ttcaggggaa
      241 aaagtcacct ttaaaataaa ttttataacg gcaatttcta agctggagta tgggaagcaa
      301 aaaaagagag ataacaatga tcaatatcta cctaaaaaat aacgaattaa tgatgcatat
      361 gttaaagttg tttttttgaa ttaaacaact caatttattc agttatatgt tcatcgcagc
      421 aagtgaacat taatcgagtt ataaaattaa taggagctaa aaatataaat aaagttcgat
      481 cagaatttat ttttccagaa gattttttta actggttttt ttatcaattc agtgaatgca
      541 agcgactctt aggtgagaat tattacttaa aaggaatcgg cggatttatg gggaatgtgg
      601 ctgatgaaca aaatattata aaaggaatat ctgaacaaac atcagagctt acagtaacta
      661 aagcatttat aagtaatggt gaaagtttga aaaatgtaac tgcgcgaatt agaaaagaaa
      721 atgaagtaga aattgttttt ggaattgacc atgaatgtaa ttcagtgata tatgtaacac
      781 aatcagcaaa tttaattctt tttgagaatg gttgcaagga cgattttatg gtggtctatt
      841 tgtacgatat actaattcca aaacttcgtt ccttatatat gtcttctgaa agtatttttg
      901 tgagtgaaca aaagggatct ttctcgaaaa aaataggtat tgaagtaatt gaatcgatta
      961 tgtgtcataa tcatattacg ctagaagaat tagaaatttt gatagggcaa aaaaataata
     1021 acacgaaaat acaaatttct tagaaatcat tttttattga gatatctaga taaatgattt
     1081 attcttcaat caaccaactc gccaagctga ggtaatgttt tgaggtaatg ataacataca
     1141 actttttata ccctagtaaa actctataaa caaagactta ttaatttcgc atgaaataca
     1201 ttcctgtaaa atgcaataaa atagagtttt gtttaaccag gtctcaaaaa ggctcgtaaa
     1261 gcatctcaat tctctaaacg ttaatattat ttatatatca atgtttcaag gcacttttca
     1321 attgtttgaa aagtgtcttt ttcttgttta agaacacatt taagaacaca tactaatcac
     1381 atttattcaa tcctctctga aaggcttcaa aaacagtttt atctactaca tctggtgtat
     1441 ttacgtatat tctcgttgta gaaatatctg agtgtgttaa agcactagat atttgattca
     1501 tatttgctcc gccttgtcta gctatagttg cataggtatg acgaagctta tgtggagata
     1561 aatgagccaa ttcaggatgt cgtcttttaa ttgaattgat tcgatagttt aggtaatcag
     1621 cgtgaacagg gcaatttact tctccggatg gtttagtata ggtaaagaga tactgatctg
     1681 gtgtttgatt aatttttaat ctcaacaatt gctcagcttg cactgatttc catttgctta
     1741 gtaatgccat tacaatttcg ggtacttgaa ttacggtatc ctttcgacct tttgtaaatt
     1801 tctttttttg atatttatcc aaagcatgtt taaggcgaac cgtttgtttc tcaaagtcga
     1861 tatatttcca ttgcaaagca tacgtctcac tttttctatc accaagatat agggtaagca
     1921 tgaaaagagt atagtcatga agagtaagca agtgattatt caagtcagtg tttactgctt
     1981 ctatccaagc aataagctct tctgacgata atgcttgatt accttcccgt atccttcttt
     2041 cttctaaagc aattttttta ggtgcagtga tacattgaat aattttggta gttcggttaa
     2101 attcaatata atttagtatt tctgcaatat caaacataga acggacatat cctttgataa
     2161 tttttatatt tgcgtattct ttcgattttt ttgttaattc atctgatatt atctcagtat
     2221 ttgaatttag atagttcatt gcatactcac caaacattgg aaggatgtgc aatcggaata
     2281 tatctttagt attactaatt gttacgtaac ttggagctct ttttgatcta gtagtctgac
     2341 ctagttcata tctaggaagc cattttgatt catagaattc cttaaatttt atttttcctt
     2401 ttaattctag tgaatttgca ttctcctctc ttaaaactgt ttcaattttc cttttcattg
     2461 cattttcggc tttaatagcc aattgcctag ttggaaatac tttttgatac ttttctgatg
     2521 taattccagt gatttctctg aatttttttg gatacctcag attagtttta tatttcccat
     2581 ttgaaagttt tgtaggcaaa attcctcact cccttaaaaa gtaaggcagt atatttcgct
     2641 attcttagta tatgcggcga gaagaaattt tcaaattgtt ttaaatgtat tcataaaatc
     2701 tttgatatcc tctatgtcaa agcgaactat accgtcaatt ctgattactg gaaaattata
     2761 tatacgaatc cacttatcta aagtattatt atcaattcct agaaactcac aggtttgttt
     2821 tttattcaag tagactctca attttataaa atcatctcct aaaaaagatt agaatttttg
     2881 aattgctttg ggtaagcaca taggaatttc acctctcccc agtttacggc gagccttacg
     2941 gaagtatcat tatttgctgc tattgtcatg gcattttgtt ttcccagaac aaaaagaaat
     3001 ctctaagtat ttgtcgctaa tcaggtcatg gcgtacttga gtttctagct cgaatagcag
     3061 ggatcgttat tcaaaaattg tatttaattg tcaatgatca aaaataggtt ttttcctatt
     3121 cactattaat aacgataaac atgtataaaa agacacacta cgaaaaaagt taaatggagt
     3181 ttactgattt tcatatttgt atatggaaca gacatagtct tgaataattt ttataaatgt
     3241 tttgatgctt atcaacttat tcaacccgct atccacttta agtataattt ttccatacgc
     3301 caataaagta gttttaggag catgttctca tttgagaacg ctcctttttt atatattttg
     3361 aaagctactt actatacgcg tagtttcaaa tagttttgtc attctaatct tcagcaacag
     3421 aaaaaaatct aaattataaa atttagggtt gactaaaata taatttttgt ataccatatg
     3481 atcgtgataa tcattatcat ttagaaggag aaagttatgt tagaagtgaa aaagcttaat
     3541 gtttgttata acgatttttt agcattgaac gagatttctt taaaaataca agaaggtgct
     3601 ttaacaggta ttattggtcc aaatggtgct ggaaaatcta cattgcttaa agctatgtta
     3661 aatattatcc ctcatcaggg agaggtctcc attaataatg aagatatcaa taaaaaactg
     3721 gtgaagattg catatgtaga gcaaaaagct gatattgatt ttacttttcc aataaaagta
     3781 aaagaatgtg tatctatggg aacatatgcc ggaatgaaag tttttcaaag aattaaaaat
     3841 gcagaatggc aaagagtttc aaaggctttg gaaaaggtcg atatgggaaa atattctaat
     3901 catcaaatag gagaattatc tggaggacaa tttcaacgtg tacttttagc aagatgtctt
     3961 gctcaaaatg cggatttcat atttttagat gaaccatttg tagggataga cttagttagt
     4021 gaacgaataa ttatggatac gttaaaagaa ttgaaacatc agggaaaaac agtgctaatt
     4081 gtgcatcatg atttaagcaa ggtaaagaag tattttgata atattattct tttgaatcga
     4141 aatctaattg ctcacggaag tgtagaatct gtatttaatg aagagaattt aaaaaaagcc
     4201 tatggagata ctatatttat t
//
LOCUS       DACEQH010000111         4218 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.119, whole genome shotgun sequence.
ACCESSION   DACEQH010000111 DACEQH010000000
VERSION     DACEQH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.119"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            10..210
                     /locus_tag="IW833_002655"
     CDS             10..210
                     /locus_tag="IW833_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ1308704.1"
                     /translation="MALGRMSELGYSMMFGEVEKNFFMKHIKGRGYVDTGGSVISEFY
                     TPLVPKGYDFLDSISKLEKMND"
     gene            288..962
                     /locus_tag="IW833_002656"
     CDS             288..962
                     /locus_tag="IW833_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1308705.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            1195..1629
                     /gene="dcm"
                     /locus_tag="IW833_002657"
     CDS             1195..1629
                     /gene="dcm"
                     /locus_tag="IW833_002657"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ1308706.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            1630..2337
                     /locus_tag="IW833_002658"
     CDS             1630..2337
                     /locus_tag="IW833_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ1308707.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            2327..2617
                     /locus_tag="IW833_002659"
     CDS             2327..2617
                     /locus_tag="IW833_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308708.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            2876..4060
                     /locus_tag="IW833_002660"
     CDS             2876..4060
                     /locus_tag="IW833_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1308709.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            4075..4194
                     /locus_tag="IW833_002661"
     CDS             4075..4194
                     /locus_tag="IW833_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1308710.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         1364 a          692 c          979 g         1183 t
ORIGIN      
        1 aactttcgcg tggctttggg tcgtatgagt gaactcggat attccatgat gtttggggag
       61 gtcgaaaaaa acttctttat gaagcacatc aaaggtcgag gttatgtgga tacaggtggc
      121 agtgtgatta gtgagtttta tacgccactt gtaccaaaag ggtatgattt cttagattca
      181 attagtaaat tagaaaagat gaatgattga gtgatcttca atactgactt tatacttcaa
      241 gtaacgtata atacttttat atagtaggaa gaaaggggat aattaatatg gaaagtaatg
      301 attcttattt agagtatgca aaagcaaata aggataagtt tatttcttat attatcaaag
      361 ggaaatcccc cgaagttgga cgagacgcag tttttatggc agggagtcct ggggctggaa
      421 aatcagaggt tgccattgga ttagctgaga attatgataa ccatgtgatt attgatgcgg
      481 attactttcg cacccagttt cctgattata atggaaaaaa ctctagtctc tttcaaaaag
      541 catcttcttg gttggtagag caggcattta gatatgtctt agatcatggc tattctttta
      601 ttctggatgc aacttttgca atattaagtg ctgaaaaaaa tatctcgcgg gcgttgaaaa
      661 aacattttcg agtgacgatt ttctatgttt atcaagaccc taaaatcgct tggaaattta
      721 cacaagaacg tgaaattgca gagggacgtc atgtgccgaa aaaggcattt attaacgctt
      781 ttttcaaatc aagagagaac attgcaaaag taaagagtaa acatccagat gtattgcttc
      841 atatcataat aaaagattat acgaataata tttccgaagt tcattatgct acggataatc
      901 tagaattatt tttaccacta gggtatagta ggaaagattt ggaggttcaa ttacatgact
      961 gagaaagaac aagtaacgaa aattgtaaag aaatacaata agagcattgc ggatttatcg
     1021 gaaaatgcta ctgcaaaaga gttcaaaacg gtgattaaat atgtagccga tcaggccaat
     1081 gaaaaacaac ggaaattagt tggattaaat aaaaaatagt aggacaaatt aataatgtat
     1141 gagccaagag caaaatgttc ttggcttttt tctttgcaat gaatgaggta atgtatgaga
     1201 tttttagatt tattcgccgg tattggtggc tttcgtttag gtatggaaca ggcgagccat
     1261 cattgtatcg gtttttgtga aattgataaa tttgctagac gtagttataa agcaattcat
     1321 gatacaagta aggaggtgga aatgcatgac atcacaagtg tatcagatga gtttattcaa
     1381 tccctcggac cagtggacat cctttgcggc ggatttccgt gccaagcttt ttcaattgcg
     1441 ggaaagcggc aaagattttc cgatactcga ggtactttat tctttgaaat cgctcggttc
     1501 accactcttc tccgacctaa gcttttattc cttgagaacg tccgaggatt gctcaatcac
     1561 gaaggagggg ctacgttcga gacgatcctc cgaacgctgg atggattggg gtatgacgtg
     1621 gagtggtagg tgcttaactc aaaggcctac attccccaaa gccgggagcg gatattcctt
     1681 atcggacatt ctcgagacgc atgtaccgaa caagtatttc ctatcattgg accgtcttca
     1741 acatctgatc aaaacatcag aaatttggta aatatcaatc cttccaatcg aggaatgggt
     1801 ggacaagtct atggttcaga tggcgtagcg ccaaccttaa caggtgatga aggaatcaaa
     1861 atcgcactgc cagcaaggga tggaatctct gtagcaggac tattaccggg aaagtttgaa
     1921 caaggtaatc gagtttatga agttactgga accgccccca ccttgtcaac gaaacaaggt
     1981 ggtacaaaaa tcatgattcg aaaaaatgaa acagaatatc aagaggtgaa gccagaagac
     2041 agtgtgaatc tggcttttcc taattccaca agtcgccgag gtcgtctggg aaaacagagt
     2101 gttcatacct tactaacagg agaccaacaa gcgatcgtca cggatcagta tcaaattcgg
     2161 aagctaacac ctagagagtg ttggcgactt cagggctttc ccgattgggc ttttgatcga
     2221 gattctcaag tcaattcaga tagtcagttg tataagcaag ccggaaactc tgtaacagtt
     2281 ccggtcattt ttgatattgc cagaagatta aaagagttgg atgtacatgg tttatgatga
     2341 gttgattgga gaaatttatt gggtgattgg aaagattcag tcagacccag aattagaaga
     2401 acaattgcga cggttaaaat ttgatattcg caagaatggt gtcaaagtcg ctggcgatcc
     2461 atatttaatg aatgaggaga cggatgctcg aattgaaata aatcaagtaa ttgcagaatt
     2521 tgagcggatt gcggaccaag caaaagaacc agatattcgt caatacttgt tagaaatgaa
     2581 agcagagttg gaagtaaatg gtattacaga tgagtaacct aaaccaacac aactgactgt
     2641 taggcgctga ccgcggagga ttcgcgaagc agaaatcctc cgtggtgctg tgcctgttca
     2701 tggacggcgt tagccgacca tgatgtctca cccccgtatc taacaggggg gtacaaaaac
     2761 acaaaaaata agtaagcaac cattaagaat gcttgttgta tcaaagatag taagtaattt
     2821 gtataggtgt taactcattt gaaaaagtta acaccttgaa tttggaggtg agaacttggc
     2881 acaacggaat ttagattatc ggctattaaa agaccgacgc aacgaatacg gcgtttcaca
     2941 aaataaattg gctacggctt gtggacttag tcggccgtac ttaaatcaga tagaaaatgg
     3001 tggcgtaacc gcatctataa aaaccatgag gaaaattttt gaccaactgg aaagctttaa
     3061 tcccgatttg cctttgacgt tactgtttga ctatgtaagg attcgctttc ccacaacgga
     3121 tgcacgaaaa attattcaag aaattctcca tttgaaattt gagtatatgc tccatgaaga
     3181 ttatgccttt tactcctatc aagaacagta tgtcatgggt gatattgtgg tgatgttgtc
     3241 tcatgaagaa gataaaggtg tgcttttaga attaaagggg cgaggttgcc gacaatttga
     3301 aactttttta ctcgctcaaa agcgtagctg gtacgacttt ttcgaggatt gtctaaaggc
     3361 cggtggcgtg atgaaacgct tggacttggc aatcaatgat cgagtggggc tattggatat
     3421 acctgagcta acgaaaaaat gtcagaagga agaatgtatc tccttgtttc gtacctttaa
     3481 aagttatcgt tccggggaac ttttaaaggc tgatgaaaag gacggcatgg gcaatacctt
     3541 atatatcggc agtctcaaga gtgaggttta tttctgtttg tatgaaaaag actatgaaca
     3601 atatatcaag ttagggattc cattagacca aactgagacg aaaaatcgtt ttgagattcg
     3661 actgaaaaat gaccgtgctt ttcatgcgat tcaagatttg ttaaaaggtc gtagcattga
     3721 gagcaccacg ttttccatta tcaatcgtta cttgcgattt gcggataaag tagaaggtaa
     3781 aaggcgaacc aactggccct tgaatgaaca atgggggcgt tttattggac ggaatcggaa
     3841 agagattcaa ttaacttccg agccaaaacc gtataccatt gaacgaacct tgaattggct
     3901 aggccgacaa gtcgccccta cgtggaaaat ggctaaggag ttggatcgat tgaatcggac
     3961 aacctatatc caggatatgg tacagaatgc acgcttatca gaccgacata aaaagatttt
     4021 ggagcaacaa agtatggcaa ttgaaaattt gattatatga aaagaggaat cgtcatggga
     4081 attgtaaaag atattttttt ggtatttggt ggcagtatgc ttggtgttac tgtgatgtgc
     4141 ttgatgaatg caagtgcagc agcggatcga gcaatggaaa agaaaggaaa gtgacaagat
     4201 gaatttcgga caaaattt
//
LOCUS       DACEQH010000112         4215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.120, whole genome shotgun sequence.
ACCESSION   DACEQH010000112 DACEQH010000000
VERSION     DACEQH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.120"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            624..3263
                     /locus_tag="IW833_002662"
     CDS             624..3263
                     /locus_tag="IW833_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1308711.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3431..4129)
                     /locus_tag="IW833_002663"
     CDS             complement(3431..4129)
                     /locus_tag="IW833_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1308712.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIAAILLIMIPI
                     ILFATTIYDGSASVSDATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVIGLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
BASE COUNT         1278 a          805 c          911 g         1221 t
ORIGIN      
        1 ttctttctgt tctgtccaaa ttattctaag atttctcttt accaacatca tttgacaaag
       61 agccagaaaa agccgagctc tcgataagct cggctttctt gtacctcgga aggattcaaa
      121 taaacagaaa tgattgtaac tttccatcat acatgcaaag cacggtgttg aatttcttca
      181 tgttaaacaa aaattttgtt ttcgttacat cttcaacatt tgaaacatct cttgttccta
      241 ttcaattttc agacgtttac gttattcaat tgttacgtat cgcattactt cttgtttcgg
      301 atacttcgtg atccaaacat ccatacgcat tcactattta taggtttgta ctctacacgc
      361 atgtctgggt gaaacctgta cttctaacaa ctttatttgc ttacactgtt ctatcgagaa
      421 attcttctgt ctatttgatg gtatcctcct tccttacatc tatataatag catcctttgg
      481 tagcgtttgc aaatataagc actttaaatt ttacgctttc ttaacaaata atcgataagc
      541 cctttatttg aagtaaataa aaaaaggatg ttatagtaaa ataagtacct ttttttgtac
      601 gaagatttta aaggaggaat aggatggaag aatacaagca gacaatagaa gaagtaaaga
      661 aggtaacaga agcagatcca gatactgggt tgtcttcaca agaagtggat gaacggcgaa
      721 aaaagcaggg attgaataaa ttcgatgagg cgccgaaaga atcgatgatc aaaaaattct
      781 ttcgaagctt atccgatttt acaacgatca tccttcttgt agcagcggtg atttcctttt
      841 ataccgcatt tgctacagaa catggggatt tatttgaagg actgctgatc atcgctatcg
      901 ttgtgatcaa ctcggtattg gcgattgtcc aagaaggaaa cgcagaaaaa gcactggaat
      961 ccttgcaaga tatgaataaa caaacggcaa ctgtcatacg agacggcaaa gtgaccactg
     1021 ttgagtcaga gcaactggta gtgggtgata ttcttgtttt agagtccggt gatgctattt
     1081 ctgccgatgc ccgtttgatc gaagcatcac aattacgtgt agaagaatcg gcgttgactg
     1141 gagaaagtga agcagttgaa aaagatgcag catttattgc tgaagaagat gagtcattag
     1201 gtgaccaact taatatggta tttaaaggat gtacggttgc tgcaggtcgt gggaaagcga
     1261 ttgtcactgc cattggtatg gctacagaaa tgggtaagat cgcggatctt ttgaacgaaa
     1321 atactatgca aaaaacacct ttacaggtcc gattgaatca gctaggcaaa cgaatcagta
     1381 tgatcgcttt agcggctgcg gctttagtat ttgtgattgg tgaactgcaa ggagaaccac
     1441 tccttgagat gttcatgact tcgatttcct tggctgttgc agctgtaccg gaaaccttga
     1501 ctgttattgt tacgttgaca cttgcatacg gtgtgcagaa gatggcgagg aaacatgcga
     1561 tcatccgaca gttgccagca gttgaaacgc taggaaccgc taatgttatc tgctcagaca
     1621 aaacaggaac gctgacacaa aatgaaatgc gcgtccgccg ggtatggagc aaagaagatg
     1681 aagtaacgaa tatcgaagat tcaatgacga acagcgcgat ggaaatattg aaaatggctg
     1741 cactatgtac agatgtgacg gtcgagcaag aagatgacga ccttgttatc aaaggtaatc
     1801 caacagaagt agccatcgta agggctgttg aagagaatta tcatacaaaa gcagaattag
     1861 aagaaaaata tccacgagta aacgagcttc cttttgattc tgaacgaaaa atgatgacaa
     1921 cagttcacca aatgggcgaa aaatacatct caatcaccaa aggagcattt gatgttcttg
     1981 ctccgcgctt tagttcaggc gatgtagaac aagcggcgat cgttaatgat cgctttggta
     2041 aacgggcact gagagtgatt gctgttggtt atgctactta tgacgaagaa ccgcaagata
     2101 tttcttctga agcactggaa aaagacttac gtttgattgg tctgatcgga atgatcgatc
     2161 caccacgtcc agaaagtaaa ggagcaatca agcgagcgaa aaaggctggc atcaaaacag
     2221 tcatgatcac tggagatcat gtggtaactg ccagtgccat agcgaaagaa ttaggtatct
     2281 tgaatgatcc gtcagaagca ctctcaggat cagaactgca tcaattatca gacgaagaat
     2341 tagatgcgcg tgtcaaagct ctctctgtct atgctcgggt aacgccagaa gacaagatcc
     2401 ggatcgtcaa atcttggcaa cgtacgggtg cagtcgtagc gatgactggg gatggtgtga
     2461 atgatgcacc agcgttgaaa gcaagtaacg ttgggtgtgc catggggatc acagggacag
     2521 atgtggcgca aagtgccgca gatatgattt tgacggatga caactttgcg acgatcgtag
     2581 atgctgttag tcaagggcgt tcagtttatc agaatattcg taaagcaatc aactttttac
     2641 taagctgtaa tatttcggag atctttattg ttttgattgc gatgctgtta ggatggggag
     2701 caccatttac agctgtgcaa ctactgtttg tcaatgtagt tgctgatgga cttccaggat
     2761 ttgctttagg aagagagcca gcggaacacg gcatcatgga tcagccaccc attccgaaaa
     2821 atgaagggat ctttgctcga ggtttattgc aaaaaattgc cataaatgca ggggtcttca
     2881 ccatcgtaac cttgtttggt tattatttag gtagttatgt cgatactatc agcccatggg
     2941 tagacgcaag tcagcatgtc ggtcaaactg ttgcattctt gatcttagct tattcatcga
     3001 ttctgcatgt gttcaatgta cgcagcagcc aatcgatctt caaagtcaat ttagcaacca
     3061 ataaagcttt ggtagaaatg gcgttactgg cattagctat tacaacggct gttgctttgc
     3121 tgccatttac gcaagaacta ttcggcctag tacacatcag tttgaatcac tggttcttgg
     3181 tagggatttt atctatcgta ccaatcgcgg tgaatgaact gatcaagttc catcgattac
     3241 cggaagcaga agaagaggaa tgattaactg tttctaagtt gattttcttt ttactggaac
     3301 tatacaaaaa ggaggtgtga catgaatgtt gtcacacctc ctttttgcta atgggctgaa
     3361 tgtttctttt actgcctctc gataaacggt gttctcctac caacccctcg gtcacaagtc
     3421 acactctctt ttaaatatct tttggcaatt gttcataaaa tgctgctttt gttgcggaaa
     3481 tgtatgggct gagccataga taaccaatac cagcagttgc taatgctaag atatgccagc
     3541 cgataaatga aagatctaac cagaaaagtt gtcctttata accatccatc aattttcgac
     3601 ttgcagtgat cgtatccagt actttaggtg tttgtcctgt ttcttggacg atatcgtagt
     3661 agataaaata gctttgagaa taagaatagc ttttaatgat tccaggaata actaatagca
     3721 aaccccataa tgttgtaaag atcgagatca gcacagctaa gccgatcact ccgccgataa
     3781 acaaaccttt gaatccacga aatgcatctt tgaatggttc gatctgatct ttttgtccac
     3841 gtaaaatatc aagataagtc caagaaatac cgctcatgaa caaagcgcta atgatgctgg
     3901 aaataattcc gccaccgcct cctgaactac cgcctcctga agttgtcgca tctgagacgc
     3961 tcgctgatcc atcatatatg gtggtggcaa ataagataat aggaatcata ataagaagaa
     4021 tagcagcaat accaatcaat gtagggatta gatttagtaa aacagcttgt ccccaacgtc
     4081 cttttagact ctcttttgct tggcgtttta attctgcaga agattgcatg atttttccct
     4141 ccttttcttt aataacacca atataccatg tatcaaaaaa tatttctgtc ataatgtttt
     4201 tctctttttt gttat
//
LOCUS       DACEQH010000113         4156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.121, whole genome shotgun sequence.
ACCESSION   DACEQH010000113 DACEQH010000000
VERSION     DACEQH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.121"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            178..774
                     /locus_tag="IW833_002664"
     CDS             178..774
                     /locus_tag="IW833_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1308713.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="IW833_002665"
     CDS             898..2697
                     /locus_tag="IW833_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1308714.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2975..3187)
                     /locus_tag="IW833_002666"
     CDS             complement(2975..3187)
                     /locus_tag="IW833_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308715.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(4051..>4156)
                     /locus_tag="IW833_002667"
     CDS             complement(4051..>4156)
                     /locus_tag="IW833_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1308716.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1228 a          707 c          729 g         1492 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg ggatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagcagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tttgtttgac gctcataatg tgtgcttaac tttctcttga ttccttcatc
     2821 gcacgaacaa tagctaaaat agcaggaatt gcagagatca ctgcaagaat aattcccatt
     2881 ttccctgaac tttttctttt tggcatatct tctcgctccc ttctgtcttt atttatatta
     2941 taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga aaccaattca gcaggtgcgc
     3001 taatatcaca gaattttacg agcatgcgat acttgctttc attgataagt tgatagtctc
     3061 gttggtcata actttcaaca tacacgacag ccgtgttgat atatgtacgt tcaatacagc
     3121 cgattagttc atgagatact ccgatcggtt tgcatatata agatcttcct acttctgctt
     3181 ttaccatttc attccctctt ttctttattt ttattttgtt ttttctttat tttataataa
     3241 caaattacct tgtatttgaa aacgttttta tccatttttt tattttttat tttgagtatc
     3301 tttctgcttt ttagataact attttttaac acgatcttag acgttgtgac aaaaacgttt
     3361 gtttatattc ctatgttgtt cgtgcgtttt tttctttata ttgctaagtt atttataaag
     3421 aggtgagcat atgatttata ttcaagcaat agtatttgta accttgatga ttgggctact
     3481 tatcggatta aaagaaaata gctaaaccat ttaagaaaaa ataaataagg aaaatgataa
     3541 atgcttttta attgcatcaa aaaacgtacg atgctaaact tggtataagt tcgaaagttt
     3601 aaaggaaact ttcaagaact aaattacatt acaaaggaga tgaattttat gacgtttaca
     3661 aaaacaaata tgcaataccg cgtagctttt gatacaactt ctaaccgttt tatggcgatt
     3721 gatgcacaaa acgaaaataa cgttgcgttt ggtattacaa tcgaacaagc agtccgagca
     3781 ttaaacgaat ctagataagg agtgaaccat tccaaatggc gactgatgag atctcggaaa
     3841 atcaataaaa gacgtttgta acggtaatcc gcgacaaacg tctttttgta ttcattacat
     3901 atatttggaa attgtctctc tttatctcta gacctaaaca aaaacacaag ttctaatatc
     3961 aaccatctcg ttttttatga caattttgtt agctgtttcg atgataatat tccgttatta
     4021 tctgtataat atttaatata atcttaattg tcaaattaag ttagacagat catcaatact
     4081 tacgtaactc aaaaatactt ccaaaggtgt tcgataatcc agtgattttc taggaatatt
     4141 atttcgttta gatgcg
//
LOCUS       DACEQH010000114         3972 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.122, whole genome shotgun sequence.
ACCESSION   DACEQH010000114 DACEQH010000000
VERSION     DACEQH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.122"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(94..666)
                     /locus_tag="IW833_002668"
     CDS             complement(94..666)
                     /locus_tag="IW833_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1308717.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(673..1335)
                     /gene="rpe"
                     /locus_tag="IW833_002669"
     CDS             complement(673..1335)
                     /gene="rpe"
                     /locus_tag="IW833_002669"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1308718.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1351..1866)
                     /locus_tag="IW833_002670"
     CDS             complement(1351..1866)
                     /locus_tag="IW833_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAQ1308719.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1868..2152)
                     /locus_tag="IW833_002671"
     CDS             complement(1868..2152)
                     /locus_tag="IW833_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1308720.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2186..3514)
                     /locus_tag="IW833_002672"
     CDS             complement(2186..3514)
                     /locus_tag="IW833_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1308721.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3528..3944)
                     /locus_tag="IW833_002673"
     CDS             complement(3528..3944)
                     /locus_tag="IW833_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1308722.1"
                     /translation="MKDQKELFQVMSEQLKNAGCVKDSFLEGITNREQEFPTGLEVNQ
                     IGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVFMLAMSQPHEQIDT
                     LQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
BASE COUNT         1274 a          798 c          568 g         1332 t
ORIGIN      
        1 attattaagt tatgtctaat caaataaaaa aaccattctc atatttgctg aataattagc
       61 atttagagaa tggtttttta tctttaaaat ctactactca aaatctgcat gtcgagcaaa
      121 catcgtttct gagctttcac ctgttttatc cattaaagat aacactaaac tatcaaaggt
      181 gagaaatgct aattgttcaa aacttgaacc cataggttgt gaaaaagtat cttcttctcg
      241 ttgattttca tctttggtaa tacctggtaa tacaagaaca gaatctgcta attgaccaat
      301 cgacgagtct tttttcatgg taactaaagc aatgcttaca ccactttgat ttgctttggc
      361 cgctaaattt tttaaactac ctgtttcacc tgagccagaa cagataatta ataaatctcc
      421 aggttgcgaa tgtggtgaac taatttcacc gacaaggctg actgaaaaac ctaaatgcat
      481 taagcgattc gtaaatgctt gcatggcaat tccagagcgt cctgcaccag ctagaaagat
      541 gtgtttagct tgaacaattt tctcactaaa ttgttctaac tctacagaat taatacgttt
      601 tgagttattt tgtaattcta agataatcgc ttctaaatag tttttatttt ggtctttatt
      661 ggtcatgatc ttttacttca cttcctctaa tgattgattt agtttatcaa tttgttcttg
      721 cggggtattt ttcccattga agatgaaacc gcctgataca aaaatatccg cccctgcttt
      781 agaacaaact ttaatcgttt gattatcaat cttgccatct acttcaatat catacgtata
      841 atcattttgt ttgcgtatta catctaactg ttcgatttta actactgtct cttgaataaa
      901 atgcttattc tctgttcctg gattgattgt cattactaat acttgatcga ctaaagataa
      961 tactggttca ataaaactga ctggtgttgc aggattaaaa gtaatcccta cttttgctcc
     1021 tgcttccttg actaactgaa tgaccttcat tgcgtgtttg gtagcttcaa tgtggaaggt
     1081 aacaatatct gctcctgctt ctacaaatct tgtgacaatt ttttctggtg tatcaatcat
     1141 caaatgaaca tcaaacacaa cgtctgttag tggacgaata tctttaataa cacttggtcc
     1201 aaaagccata tctggtacaa aacagccatc cattacatca atatgcaaat aatcaacatt
     1261 cttttctttt aagatttcaa ataactcttt aacttctgcg ataggtgcat ttaacaatga
     1321 tggagcaatt gtcattctct attcccccgt ttattctccc ataatcatga gattgattgt
     1381 tctagttcgc tctcttagtt gatcccaatc gacaaaatag acacggccca tttctcctaa
     1441 taaaagttta ccttcttgaa caattaaagt cattgagttg gttccataaa tagatgaacg
     1501 taaatgtgca tcggtattta gcatgaccca tttttctgca gggtaattgg gatcagataa
     1561 tcttgttcca aaatcaatgt gttttggccc cggactgttg tattgatttt cactcgtcat
     1621 ttttggaata atcgtttcca ttacattgtt gatgtctaca tgtaaataat catcaccaaa
     1681 aaagtttgtg tcatgcatcg tttcttcaaa aaagagtgag caagttgtat gcgggctcga
     1741 aattactaac attccattct caatacctgc tgtatcaact gtatctaaaa gttcttttcg
     1801 aatatcatga tacgaaggac gccctgaaac tgtttcaata gtcaaagtgt catgatgaaa
     1861 acgcatatta aacacctaat ctttctttta catctgcata aatggcatct gtaccaatac
     1921 ctgttaataa gcccaagcca ttcgttactt tagctttaat tacatcaggg acaattgttg
     1981 tacttactac agcatcatat tcatcaattt tatttagctc atcagcaatt ttagattgat
     2041 aaattttcac ttgatctgtt aatccttctt cttccaacca ttttgataat tttgctactg
     2101 caattgtaga agtagcatga cctgctccac aaataactaa taattttttc atattgatct
     2161 cttctttctt attttaattt tatgattaag ctttcttcac tttgttcatg tagatagaag
     2221 cacctactaa aacaactcct aatccaccta ttccaattac accaaagtaa gatgctaata
     2281 aaacaaacaa ccctactggc cataatccac ataacaacat agttactaat cctgctgtcc
     2341 ccacatcgaa acttgctaat gtaaacacat cagtgataat tggtgccaac catgttgcca
     2401 tatataatgt tgtaacaaca tacaacgaac atcctactaa tgatctcaca atatttcctt
     2461 taaaataagg aatcattaaa cagatggcga aagcaaagaa tgctaaatct ccaaaaggta
     2521 atactttatt tcctggaaga attagtgcta gcaataatga gattggtaca agcaatagag
     2581 aagttgacat aatagttgga tgacccacag ttaacgctgc atccattcca atgtttactt
     2641 ggcgaccatt taacttttta ttcgtaaatt ctttcgccgc ttctgcaatt ggcattaaac
     2701 cttccataaa cattgctacc attttaggca taatcttcat cactgccgca gtcataattc
     2761 ctaagtttcc aattcctgct acgtcatagc ctgccataat acccactaca ataccgataa
     2821 ttaggccaat aacaactgtg tcacctaaaa caccgaaacg ttttgttaat gtttctgcat
     2881 ctgcatctat tttattaaat ccaggaatac gatcaaaaac ccaatttact ggtttggcaa
     2941 atagtactcc tgataatccc atcatatgtg tgatagagat tcctggatag ccgaaaaatt
     3001 cgctaacagt tttttgtgta cgatctgcta agaaccattg taaaattact gcgacaatcg
     3061 cgccaattaa gccaaaccaa aagtttcccg taacagattg agtaatcata cctaggaaaa
     3121 ttgcgaacca aaagttccaa atatcaacat tcaatgtttt tgtccaaccc aaaaccacaa
     3181 atatgaagtt aagaattaat gaaattggaa tcattaaaat tcccaatgat gtagagtaag
     3241 ccaaaggtcc accaacacct acgccaatat ctaggatatt caggccacca ccgtattgtt
     3301 cccccatttg ttgaattgct gttcctaaac catctgataa taaacttaaa actaatccta
     3361 aaccaataga accgatacca acagtaacgc cggaaacaaa tgctttacca ggttttaatc
     3421 caaataatat cccgattaca aaaataatca gtggtaaaaa gacgtttgac cctaatccaa
     3481 taaaccattc aaaggcattt aatacaatat ccatctctct attcctccta ataaactcct
     3541 gcagaatgta ctaactcaat aaatgcttct ttcgtggtac atgcaagtaa ttcatttact
     3601 ttttcttcat tttgaaatag gctaactaag ttttgtagtg tatcaatttg ttcatgaggt
     3661 tgtgacattg ccaacataaa gactaaacgt acaggtattt catgagattt atctgtcata
     3721 tctgaaaata ccagtggttc ctttaatgag gcaaaacaga tttgagagct atttacatgt
     3781 gcggaatctg tatgtgggat agcaaaccca atttgattta cttctaatcc agttggaaat
     3841 tcttgttcac ggttggtgat accttctaag aaactatctt tcacgcaacc agcgtttttt
     3901 aattgttcag acattacttg aaacaattct ttttgatctt tcacttctaa ttgaaataaa
     3961 cataattctt ta
//
LOCUS       DACEQH010000115         3966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.123, whole genome shotgun sequence.
ACCESSION   DACEQH010000115 DACEQH010000000
VERSION     DACEQH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.123"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..430
                     /locus_tag="IW833_002674"
     CDS             <1..430
                     /locus_tag="IW833_002674"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAQ1308723.1"
                     /translation="IGIVIARFNDFLTKSLLSGCEDGLTRSGVLTENIDVAWVPGAYE
                     IPLTAATMAETGKYDAIITLGAVIRGATSHFDVVVNQASSGVSQVGLTHKLPVIFGIL
                     TVENIEQAIERSGTKAGNLGFTYATNTIEMINLFKEIKQQ"
     gene            580..1959
                     /locus_tag="IW833_002675"
     CDS             580..1959
                     /locus_tag="IW833_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAQ1308724.1"
                     /translation="MTIQFKQTLAPTKDNSGKPIILSDATFAQRNEKVLQLMREYDFS
                     SLMIYADKEHGSNFEYLTGFIPRFEEAIQVINQDGTSTLILGNENYNKTSMARTQSEG
                     ILCPFFSLPNQPMDFTKTFADYLKDVKVDASKRIGLVDWKLLSNDMDNFHNHHSIPAF
                     IVDGIHEVFGSDKVVNATQLYMHPKEGARVTNNAEEIARYEYGASLASDAVLEAMNQL
                     TVGQSELAAGNHLNRDGQYPSVVTIAAFGDRFEGANIYPTDNTLNDGDKVALTVAYKG
                     GLSSRSGYAVKEESQLAEHDPGYLEEVVYPYFQAYKWWLDNLTIGRKGGDFYQAFSDF
                     YPQATYGWNLCPGHLVADEEWLSSPFYQDSDATIQSGYIFQLDFIPSQAGHNGVSAES
                     TIAVADAALREQIKAEQPELWARICNRKDYLKEALGLELSEHLLPLASTFAYYRPFLL
                     DKKTAIYFG"
     gene            2096..2938
                     /locus_tag="IW833_002676"
     CDS             2096..2938
                     /locus_tag="IW833_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1308725.1"
                     /translation="MPLDEQIELLFTIDEGYLNPLKVALTSIRQNNPGQAFRIWLIHE
                     SIAMHTIRELQKLTDYLQFGFEAIKIDGSRWNSAKTEDRYPKEMYFRLLAGEILPKEM
                     KRVIYLDPDILVVNPLLDLWQTDLEGYMLAAATHVGLTDVSTRVNQMRLDVDHAYYNS
                     GVMVIDLDKAREIIKWSDIAQMIEKYNLLLILPDQDILNRLYGKYTKEIPEEIWNYDT
                     RKYMRYFTKSLAQHDIHWVMAHTAILHFCGGPKPWDDKHDNRFTSLYLTYQNQLKRIE
                     DLMK"
     gene            3081..>3966
                     /locus_tag="IW833_002677"
     CDS             3081..>3966
                     /locus_tag="IW833_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ1308726.1"
                     /translation="MAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTMEDPDFPRIAI
                     TYSLEENNQDSSAQQEEMKKIIEEYNQYFDTAWSLADIERYNGDINNCLARKRAEFKQ
                     FGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVT
                     FRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMKSIDEH
                     TPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIGVYETVKG
                     SLIEEEPEKEGEDFSTIEF"
BASE COUNT         1311 a          707 c          806 g         1142 t
ORIGIN      
        1 aattgggatt gtcattgcac gattcaatga ttttttaact aaaagcttac taagtggttg
       61 tgaagatggc cttacgcgtt caggcgtttt gacagaaaat atcgatgttg cttgggtgcc
      121 gggggcctat gaaatcccac ttacagccgc aacgatggct gaaacaggta aatatgatgc
      181 cattattacc ctaggtgcgg taatcagagg agctacaagt cactttgatg tggtggttaa
      241 ccaagcgtca agtggggtat ctcaagtagg cttaacccat aaattaccgg tcatctttgg
      301 tatcttaacc gttgaaaaca tcgaacaagc cattgaacgc tctggtacta aagctggtaa
      361 ccttgggttt acttatgcaa cgaatactat tgaaatgatt aatttattca aagaaattaa
      421 acaacaataa gtcatcattc tatatgaaaa tatctgtaac agcatagggc tagctttaat
      481 tttcaatcat ggtaagatgg aagataggaa aataaagcgc attcaattaa ttatcaagcc
      541 tttctgatga tttgcgcaaa aagcttaagg agacttcata tgacgattca atttaaacaa
      601 acattagcgc caaccaagga taattctgga aaaccaatca ttttaagtga cgctaccttc
      661 gctcaacgta atgagaaagt ccttcagtta atgcgtgaat atgacttctc atcattgatg
      721 atttatgcag ataaagaaca cggtagtaac tttgaatatt taactggttt tattccacgg
      781 tttgaagaag ctatccaagt aatcaaccaa gacggaacgt cgacactgat tttaggtaat
      841 gagaattaca ataaaaccag tatggcacgt acacaaagtg aaggtattct ttgcccattc
      901 ttctctttac caaaccaacc tatggatttc actaaaactt ttgctgacta tcttaaagac
      961 gttaaagtag atgcttcaaa acgaattggt ttagtagact ggaagctttt atctaatgat
     1021 atggacaact tccacaacca tcattcaata ccagcattta ttgtagatgg tatacatgaa
     1081 gttttcggtt ctgacaaggt agtgaatgca acccaactat acatgcatcc aaaagaaggt
     1141 gctcgtgtga caaacaacgc agaggaaatc gctcgttatg aatatggtgc ttcccttgct
     1201 tctgatgctg ttcttgaagc aatgaaccag ctaaccgttg gccaaagtga gttagcagca
     1261 ggtaatcact taaatcgcga tggtcaatat ccatctgttg tgaccattgc agcctttggt
     1321 gatcgctttg agggtgctaa tatttatcca actgataata cgctaaatga tggcgataaa
     1381 gttgcattaa ctgtagccta taaaggaggc ttgtctagcc gctctggcta tgcagtaaaa
     1441 gaggaaagtc agctcgctga acatgatcca ggatacttag aagaagtggt ttacccatat
     1501 ttccaagcct ataaatggtg gctagataat ttaaccattg ggagaaaggg tggcgatttc
     1561 taccaagcat tcagtgactt ctatccgcaa gctacttatg gctggaattt gtgcccaggg
     1621 catttagtag ctgacgaaga atggttaagc tcaccatttt atcaagactc tgatgcaaca
     1681 attcaaagtg gctacatctt ccaactagac tttattcctt cacaagcagg ccacaatggt
     1741 gtttcagctg aatcaacgat tgcggttgcg gacgcggcac tacgtgaaca gattaaggct
     1801 gaacaacctg aattatgggc acgtatctgt aaccgaaagg attatctaaa agaagcatta
     1861 ggtttagaat tatcagaaca tttactaccc ttagcgagta cctttgccta ctatagacct
     1921 ttcttattag acaaaaaaac tgctatctac tttggataaa aaaatgcccc tcaagattgt
     1981 ttgccaaact tgagggtttt ttgtctattt ttaagtgaat aatgacggac tatccaaacg
     2041 gaatcgccat aaagatgtta atatatgtat aatattataa agaaattgga ggacaatgcc
     2101 cttggacgag cagattgaat tattatttac catagatgaa ggctatttga atcccttaaa
     2161 agtggcttta acatcgattc gtcagaataa tccaggccaa gctttccgca tttggttgat
     2221 tcacgaatcc attgcaatgc atacgattcg cgaattgcag aagctaacgg attatctaca
     2281 gtttggtttt gaagcgatta aaattgatgg tagtcgatgg aatagcgcta aaactgaaga
     2341 tagataccca aaagagatgt atttccgcct gcttgcgggt gaaatattac ctaaagaaat
     2401 gaaacgcgtg atctatttag acccagatat tttagtggtt aaccctttat tagatttgtg
     2461 gcaaacagat ttagaaggtt atatgctagc agctgctaca catgttggat tgacggacgt
     2521 ttctacacga gtaaaccaaa tgcgacttga cgttgaccat gcttattaca attctggtgt
     2581 gatggtgatt gatttagata aagctagaga aattattaaa tggtcagata ttgcacaaat
     2641 gattgaaaaa tataacttgc tattaatcct acccgaccag gatatcctca accgattata
     2701 tggtaaatac actaaggaaa ttccagaaga aatttggaat tatgatacgc gtaaatatat
     2761 gcggtatttc accaaaagtc tcgctcaaca tgatattcac tgggtgatgg cgcatactgc
     2821 tattctccat ttctgtggtg gcccaaaacc atgggacgat aaacatgata atcgttttac
     2881 cagtctttat cttacctatc aaaatcaatt aaagcgtatt gaagatttga tgaagtaaag
     2941 tgaaacaagt gtttttcatt acctacggtg gtttaatgct tatttattat cgttaaatca
     3001 tgcttataca ctttacaaaa tttgattttc gaaatggccg tccaacgaag tctgcgatct
     3061 taaccacgag ttccattgac atggccaaaa agtattatcg agcgatcaaa gaaatgacga
     3121 aagaacctga ctggttaaca aaagagtttc ctaatcagcc cattcgtgaa ggaagaacga
     3181 tggaggatcc tgattttcca cgaatcgcta ttacctattc attagaagaa aataaccaag
     3241 attcaagtgc ccaacaagaa gaaatgaaaa aaattattga ggaatataat cagtattttg
     3301 atactgcctg gtcattagca gacattgagc gttataacgg agacatcaat aattgtttgg
     3361 cacgaaaaag agcggaattc aaacaatttg ggaaacaaat cgatttagta atcgtagtgg
     3421 atcgtctatt gacaggtttt gacgcaccta cgattcaaac cctatttgtg gatcgtaatc
     3481 tagagtatgc aggattgatt caagcttttt cacgtacgaa ccgtacgtat cctgaaaaga
     3541 caaaaggatt gattgtgacg tttcgtaagc cagcgacaat ggagaagaat gtcgaggatg
     3601 cgacaaaact gtattcagaa gcaaaagaag aatcgggttt gatttatcca agttatcagg
     3661 aatccaaaaa aagattcaag caagcgtatg gtaagttaaa ggaggtttca tccatgaaat
     3721 ccattgatga acatacgcca cttgaaatcc gtatcgagta cgttaaagca tttcaagaat
     3781 tgaatcgtgc gtatgaatcg ttagtgactt atgacgatta caatgatgac atggtgcagt
     3841 cttcaacttt gaacaatcaa attcaacttt tggaagaaca aattggcgtg tacgagaccg
     3901 tgaaggggtc attaatagag gaagaaccag aaaaagaggg agaagatttt tcaacgatcg
     3961 aatttt
//
LOCUS       DACEQH010000116         3883 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.124, whole genome shotgun sequence.
ACCESSION   DACEQH010000116 DACEQH010000000
VERSION     DACEQH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.124"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            82..3123
                     /locus_tag="IW833_002678"
     CDS             82..3123
                     /locus_tag="IW833_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ1308727.1"
                     /translation="MIHLVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDC
                     QKLLGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRF
                     KVAYFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKN
                     QYTGQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPF
                     NQGSNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDRE
                     VTKKTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIH
                     DQLNYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGR
                     KIIITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEY
                     YELEGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFE
                     PFHVYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLH
                     PWAVTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDL
                     DVLVAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGF
                     DEPLLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQ
                     TNLREEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKP
                     IMDRYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKE
                     TRENIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEII
                     HKINERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDK
                     VMEQYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
     gene            3313..>3883
                     /locus_tag="IW833_002679"
     CDS             3313..>3883
                     /locus_tag="IW833_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAQ1308728.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFIS"
BASE COUNT         1329 a          621 c          824 g         1109 t
ORIGIN      
        1 atagctgaat tgatggaact tgagagttat aaaaaagctc tcatctacga atacgtaacc
       61 ggtaaaaaag aagcctagga ggtgattcat ttggtagaga acaaagaaat tcgttttgag
      121 caggatattg agacttcact gactacgtat ggcaaatgga agaaagaaac attcaatgaa
      181 tcaaactttg atcccataat tggtttggat tgtcaaaaat tgctaggctt tgtccagaag
      241 actcaaccaa aacagtgggc acgtcaccaa cgaaactata acaatgatgg agaagtcaag
      301 ttccttcaac gctttaatca agaggtagaa acccacggca tgttacatgt attgcgcaat
      361 gggatcaaag accgcggtgt gcgctttaaa gtagcttact tcaaacctgc ttcagagctg
      421 aatgatgaaa cgatggaaaa gtatgaagca aatagtttta cctgtactcg ccagtttgct
      481 tacagtccta ataaccataa aaccattgat atggttttgt cgttaaatgg tattccgctc
      541 gttgctttag aattgaaaaa ccagtacact ggtcaaacag tagaaaatgc caaactccag
      601 tttcaaaaag atcgtgatcc aaaagaattg gtatttcgtt ttaatcgccg cttcttagtc
      661 tattttgcgg tggaccatac agatgtttat atgacgacaa agttggacgg taaaaaaacg
      721 tacttcttgc cattcaatca aggttccaat gggccgggaa gagttggtgg agcaggcaat
      781 ccgtccaata aagatggcta cccaactgcc tacttgtggg aaaaggtgct tactagagac
      841 aatttgatgg atattatcca acgttttatg aatctagaga ttaaaaaaga aaagcaaggc
      901 gacagagaag tcacaaagaa aacgttgatt tttcctcgtt atcatcagtt agatgtggtt
      961 cgaaaattgg ttgcagatgt aaaaagccat ggtagtggag aaaattattt gatccagcat
     1021 agtgctggtt ctggaaaatc taatagtatt gcatggctgg cataccattt agcctctatt
     1081 catgatcaac tgaactaccc aattttttca tcaattatta ttgtgactga tcgaacagtg
     1141 ttggatcgcc agttgcaaaa tactatcatg aactttgatc atcagacagg tttggtagaa
     1201 acgattgacg atcgcaaatc ctctcaagat ttaaaagaag ccattaatga tgggcgtaag
     1261 atcattatta ccactttgca aaagttccca gtgatctatc aggaagtaga tcaagtggaa
     1321 ggtcgtaatt ttgctgtgat tgtggatgag gcacattctt cacaaactgg gaacagtgcc
     1381 cagaagctaa aagccgctct ttcggacaaa tctaaatcct tggaagagta ttatgaactg
     1441 gaaggcgaag ttgacgatgt attaccagat gctgaagatg aactagttca acaattggta
     1501 gcacaaggta aacatcaaaa tttaagtttc tttgctttta ccgctacacc gaaagaaaag
     1561 acattggaaa tgtttggtac gaaacaacca gatgggcatt tcgaaccctt tcatgtttac
     1621 tcgatgcgcc aggcgattga ggaaggcttt atcttagatg tgctgaagaa ctatatgacc
     1681 tatagtactt cctacaaaat tgcccgtgaa attgaagata acccagaatt accaaagagt
     1741 gaagcaaccc gagcgattgt tcgtttccaa tcattgcatc cttgggcagt tactcaaaag
     1801 acggctgtga tggttgagca attccgagcg gttactcgta cagctattgg tgggcaggca
     1861 aaagcaatgg tcgttaccgc atcacgctta cacgctgttc gctatatgaa agaatttaaa
     1921 aaatatatag cgaacaaagg ttataatgac ttggatgtgt tggttgcttt ttctggttcg
     1981 gtagtagatg gagagactga atatacagaa acgaagatga atgtcacaaa atctggtgag
     2041 aatgttcggg agaatcaatt aaaggaagct ttccatacag ataattttaa tatcttgatt
     2101 gttgctgaaa aatatcaaac gggttttgat gaaccgttgc tgcataccat gtttgtcgat
     2161 aagaagttaa agggtgttaa agctgttcag actttatcac gactcaatcg aacaatgcct
     2221 gggaagaagg atacctttat tcttgatttt gtaaatgatg ttgaagatat tgaagctgct
     2281 ttccaaccct tttatgaaca aacaaatttg agagaagaaa tcaatgttaa cttgatttat
     2341 gatactcaaa gcaagctacg aaaatttaat gtttacaatc aaggtgatat tgaacgtgtc
     2401 atgaaattgg tgaaacaagc ccaaaagcaa cagaatgagc gcttgcttgg acgaatttct
     2461 agcatgttta agccgattat ggatcgctat gaggaaatga acaaggataa tcagtaccag
     2521 tttcgggtga ctctacgtaa ctttggcaag tggtatgatt atatttcaca acttgatcgg
     2581 ttattcgata tggaactatt ggaggaacga atttttactg gatacttgct gaagtttcta
     2641 cctaaagaaa cacgagaaaa tattgatatt tctgacaaag taaagttgga atattataag
     2701 ttacgtcagg acttttccgg tgaaatccat ctagtgaata aagaaggcga gctagtaaat
     2761 ccaaagtcaa ttgatgcagg aatcaaaccg ccggatgaac gagattcact agaagaaatc
     2821 attcataaaa tcaatgaacg atttccaaat gattttgacg aaggagaccg ggtgttagta
     2881 gagattttgt atcggtcatt tgccgataac ccagatttga aggttgtcag catggctaag
     2941 aataacgatg ctcagatgtt tgaaaaatcg ctgttccctg atgtatttaa agataaggtg
     3001 atggagcagt acgagaagaa caatgcagct tttgagaaaa tgtttgggca tgatgataag
     3061 tactttgatc tagtatatac attagttgct agagatttat acaagttatt acggacgaaa
     3121 taaaatttat aaataaggat tagacaatac aatttctctc aaaaataaag gagaagttgt
     3181 attgtttgct tttaagaaag ccctgaaaaa tttgttacgt actaaattgt ctgaactctg
     3241 tcatatcagc tatgtcagaa taaaggaaat agatttattt ttataaacag tgtcgtctcg
     3301 gagatttaaa ctatgtcaac gacaattgaa gtgaacaaac aaagcgtgaa gcagtttctt
     3361 gaaacaggga aaatcaaaaa attcgttatt ccagaatatc aacgtcctta tgcctggact
     3421 gacgagcaaa accaagtact atttgatgat ttagcggagt acacagcaaa caataacgag
     3481 agtacttact ttttaggaag cattgtggct tatgaaaatg atcataacga gcaagaaatc
     3541 attgatggac agcaaaggat aactacgcta tttttatttt tgagagctat ctatgctaaa
     3601 ttagaaaata gttgtgaaaa ggaagcttta ttcttgaaaa gccaaattga acccgcgtta
     3661 tgggaacaag atgatttaac aggtgaggtt aaaccagata aaattctgat tatgtctcga
     3721 gtaatggggg atgaggggaa tgaagaattt gctagtattc tagtaagtgg tgaagcagat
     3781 gttaaatcaa aaagtaatta ttcgaagaac tatattttaa ttcaacatct attaaatgag
     3841 tatgcaacaa acgaaccact atcattttat cgttttatca gta
//
LOCUS       DACEQH010000117         3814 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.125, whole genome shotgun sequence.
ACCESSION   DACEQH010000117 DACEQH010000000
VERSION     DACEQH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.125"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            98..2197
                     /locus_tag="IW833_002680"
     CDS             98..2197
                     /locus_tag="IW833_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1308729.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2393..3256
                     /locus_tag="IW833_002681"
     CDS             2393..3256
                     /locus_tag="IW833_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1308730.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3361..>3814
                     /locus_tag="IW833_002682"
     CDS             3361..>3814
                     /locus_tag="IW833_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1308731.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         1525 a          636 c          772 g          881 t
ORIGIN      
        1 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
       61 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      121 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      181 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      241 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      301 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      361 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      421 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      481 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      541 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      601 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      661 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      721 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      781 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      841 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      901 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
      961 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1021 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1081 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1141 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1201 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1261 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1321 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1381 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1441 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1501 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1561 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1621 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1681 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1741 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1801 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1861 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1921 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     1981 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2041 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2101 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2161 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2221 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2281 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2341 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2401 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2461 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2521 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2581 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2641 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2701 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2761 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2821 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2881 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     2941 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3001 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3061 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3121 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3181 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3241 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3301 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3361 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3421 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3481 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3541 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3601 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3661 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3721 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3781 catggaatct gtcaggaatg ctatgaaaaa gaaa
//
LOCUS       DACEQH010000118         3743 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.126, whole genome shotgun sequence.
ACCESSION   DACEQH010000118 DACEQH010000000
VERSION     DACEQH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.126"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..144
                     /locus_tag="IW833_002683"
     CDS             <1..144
                     /locus_tag="IW833_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1308732.1"
                     /translation="YRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLG
                     IPA"
     gene            complement(760..1800)
                     /locus_tag="IW833_002684"
     CDS             complement(760..1800)
                     /locus_tag="IW833_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1308733.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2149..2475)
                     /locus_tag="IW833_002685"
     CDS             complement(2149..2475)
                     /locus_tag="IW833_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308734.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2462..3250)
                     /locus_tag="IW833_002686"
     CDS             complement(2462..3250)
                     /locus_tag="IW833_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1308735.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1148 a          623 c          526 g         1446 t
ORIGIN      
        1 tatcgaagtt tacgcactgc ctctaccacg attaaaggca tggaagccat tcgaggatta
       61 tataagaaaa cccgaaaaga aggcactctc ttcgggtttt cggtctgtac tgaaatcaag
      121 gtattattgg gaatcccagc ttaaatcata gataccgtaa gggattttat tctttattta
      181 aaactttgca acagaaccgt ccgttgcggg taataccgta acactgaagg gcacgcatta
      241 aggcgtcatc ttaatgcttt cctgtaggac actgacggag attaccagtc tcccgagcct
      301 tacaggggcc tttctttttt ttgtattttc aattatcaca atttatcgtc caaaagtgca
      361 gaacgaaaat caattcatca caagcatatc agacaattaa atttaaaaca actaaaaata
      421 tacaaaaaag tgtaattgaa tattagcagc actgcctgaa aagaagtaat atcaggcttt
      481 tcagcttggg gaaataagtg aattacctct cttcatgaat tattataact caaaaaagca
      541 catatgcaaa cactagttcc ctattttttt gaatgaaaaa taactaaaat atctaacgat
      601 tatgacttta aaacctacgt ttgtgattgt tttcataaac ttttaatcta aaaactccgg
      661 aataatagct atttatttgt aacatttgta ataaaattaa aatcataaaa aagttctaat
      721 ttaaaattga tcatttttat agtgatcgct ttttatcaac tactctagcc aattttccgt
      781 actaactgtt ggtaattcgt taaatgtttc tctttgttcg tttgcaagct ttgccgcaat
      841 agctttttcg acaaaccaat tttttacata aacgaaaatt agattctgca aattgtttac
      901 tttttcagtt ttttgttttt gataagcttt cagcaaagtc atatataatc ctttgtcagc
      961 atcaattcca taagattcca gttcttcata aacaacaact gtttgctcta attcttcagc
     1021 ttttttcttt gcattatgaa tagtttggac ggttttttga gcgtcttcgt aagtactaga
     1081 aaatgttttt atcaagttta atacattttc tggtacaaat gtatttatac tgtcctcttt
     1141 catgatgtcg acgaagttat ctagtaaaac ttgattttgt aactgatcag tttcagtgtc
     1201 tattatgtgt ctattagtgt ctatagaatt agaatagaat agattgtctg cgattttcgc
     1261 aattacggtt gagtccttga tatgactgga ttttgccgta tttgcgattt tcgcaattac
     1321 ggtggtatca ttgatttctc gcggttttgc cgtatttgcg attttcgcaa ttacggtggt
     1381 atcagtattg tcttcctctt ccatgatttt aaagacgtct tgattcgtta cttctggctt
     1441 gcctaagtac aataaattag gcatggattc aatcttaccg tcacgtttat ttactcttcc
     1501 tctcttctgt ttcagaagac ctgctttttc taattccttt ttgattttag atactttacc
     1561 ttcgcggcat tgtaacaatt tcattaattc ttcaactgtg aatatgaaaa atatattatc
     1621 atcagaatct acccagttat ttttgactga ataatcaaat cgatctttaa gtagcatata
     1681 agcaattttt gcatcatttg atagatccat gtatttttcg ttagtaaaaa ataccttagg
     1741 catttgataa tatttttcac gataaatttc ttttgatttg taataattaa aatcattcat
     1801 agccaatcct ctttctgtag gatagcgttt tgttcaaaac tatgttacaa tgaattaaac
     1861 aaatatatct atccactata tatttttcta ctgctgataa gtcgccaaac tttgttaaag
     1921 cagtagtctt tttttatctt tttttagttt accttaaaaa ataaattaaa gcaaaccgct
     1981 ctatttcaag gtttaagctc atagtaacta tatcacgata ttacaataat attatatcac
     2041 gatatcgtaa tattacaata tgaagataag cgacttatca gctttgatac tactgatttt
     2101 tataaaaaaa atatcacgat attatgatat cgtgatattg aatgtaaatt aaattttatt
     2161 ttgtttcttt gctagtttat atccttcaat caagtattct tcaggtatgg actttaatag
     2221 cgcatcttct ataacgtctg gtttactttt gtctgattca gcaatagtcg tccaatattt
     2281 gagtgctgac attagaattg gatttatacg aatgctatct gctgttctta cactacttcc
     2341 ttgatctttt atgactttac ttatattttt cttggttaat tcgatttctt ctacttcttt
     2401 tgatttaaaa ttctttttat ttttatttcc tagtaatcta cttactgcag cttgactgcg
     2461 attatcttct gccatcaaaa tcaccaactt cttttaaata ttcttctgta aatgctttat
     2521 agtcatcttc agctgttgag ggtttattaa aattgattag atctgatcca gcagcttgcg
     2581 cttcgtcaac ggttattgtc ctacgaatat aagttttgaa aatttttgca ccaatgactc
     2641 tagaaatttc ggttgctgca gacgtcattt ctttagaaat gttataacgc ttattatact
     2701 tagtaaatag aactcctcgt acttcaagct tgtcgttcgt atattctttt accgtttcaa
     2761 tagtttcact taactttatt agtcctttta atgagaaata cgcagcttca actggaatga
     2821 taatttcatc agaaacagta aatgcgttga ctgtaagcat tcctaaactt ggtggacaat
     2881 caattataat ataatcatag ttgtccttga ttggttgaag tactctttta agcctgtgtt
     2941 cacttcctac ttgagtaaat tccctctctg cagaagaaag ggctatgtcg gctggcaaaa
     3001 catcaattcc tttatagtcc agaatagcat ctgtaatgtc agcctcccct tttaatactt
     3061 cataaattgt aggttggttt gttccatcta tatcgaaacc tacattatct gtagcatttc
     3121 cctgaggatc aatgtccact aaaagtacat ttttaccgta aacaaatttc aaacatgctg
     3181 ctaaattaac gctagtcgta gttttagaaa ctccaccttt ttgattggct actgacaata
     3241 ttcttgccat taaaatatcc cccattcatc ttatattata atataattat atcataatat
     3301 tgtgatattg caatatcgta tatgcattat attgtaatat tgtgtgatta ttttgtttta
     3361 aagatatata tcggttcgga tataaagatt attcatatgt tattcgaaat taaaataatc
     3421 actttttatt actttaaaat tttttattaa tgataagaaa tatgttttgc gagcagcaac
     3481 tagaataatt taaacattac taaactgtct atattgttac taataattcc cttcttatag
     3541 tgcattttct tagaaaaaaa caatattacg atatcatgat attaagatat cgtaatatta
     3601 tacaattgtg attgatatta tatatgtttt tttatttttt tatcatgttt aatcttgaag
     3661 gaatcatatt ctttttctaa agacaggagc ttttcataaa catcagacat gtttaaacta
     3721 gaggccaggc ttaaatcata aac
//
LOCUS       DACEQH010000119         3646 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.127, whole genome shotgun sequence.
ACCESSION   DACEQH010000119 DACEQH010000000
VERSION     DACEQH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.127"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            41..775
                     /locus_tag="IW833_002687"
     CDS             41..775
                     /locus_tag="IW833_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1308736.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            791..1960
                     /locus_tag="IW833_002688"
     CDS             791..1960
                     /locus_tag="IW833_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1308737.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            1976..2971
                     /gene="lacD"
                     /locus_tag="IW833_002689"
     CDS             1976..2971
                     /gene="lacD"
                     /locus_tag="IW833_002689"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1308738.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3050..>3646
                     /locus_tag="IW833_002690"
     CDS             3050..>3646
                     /locus_tag="IW833_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ1308739.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVD"
BASE COUNT         1201 a          627 c          813 g         1005 t
ORIGIN      
        1 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
       61 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      121 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      181 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      241 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      301 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      361 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
      421 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
      481 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
      541 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
      601 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
      661 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
      721 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
      781 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
      841 tacccgagaa attcgacagc aaccggaact gtggcatgag acgtttgacc gttatcaaga
      901 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
      961 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1021 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1081 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1141 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1201 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1261 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1321 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1381 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     1441 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     1501 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     1561 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     1621 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     1681 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     1741 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     1801 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     1861 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     1921 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     1981 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2041 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2101 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2161 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2221 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2281 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2341 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     2401 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     2461 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     2521 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     2581 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     2641 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     2701 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     2761 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     2821 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     2881 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     2941 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3001 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3061 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3121 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3181 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3241 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3301 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3361 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     3421 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     3481 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     3541 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     3601 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagat
//
LOCUS       DACEQH010000120         3416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.128, whole genome shotgun sequence.
ACCESSION   DACEQH010000120 DACEQH010000000
VERSION     DACEQH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.128"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1258)
                     /locus_tag="IW833_002691"
     CDS             complement(<1..1258)
                     /locus_tag="IW833_002691"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ1308740.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIR"
     gene            1443..3389
                     /locus_tag="IW833_002692"
     CDS             1443..3389
                     /locus_tag="IW833_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ1308741.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1080 a          608 c          578 g         1150 t
ORIGIN      
        1 cacgaatcgt accaaagtca gtggcatatt gaatgcctgc tttgctgcga tctaaaataa
       61 tttttccttc tttggtatct acaatcaagc taagtccatt tcctttacta tcagagaaaa
      121 gaagtaactc cgatttctga tttggaggaa aagttagttc aagctcataa gtattatttg
      181 tttctgcttt tgctgtaaat tgttcagaac tagcacgtaa tgatgtaata gcttctactg
      241 gatattggta aagtttacca tcttttattg aaagttcttt tacaagactc attgctccct
      301 gataatcata cttatcagtt ggataatcga tatcaggtag tccaatccaa ctgacaatca
      361 gagcacgacc atcaggagta ttgaatcctt gagtcgcata cgcttcaaat ccataatcaa
      421 gattttgaat ttctgaagcg cccacaagtt ttactttttc tatgtcaagc gattgacaga
      481 tcttaaaagt attcggatag atattcccat aatttagctc agatttatcc aacccttgag
      541 gacaataaat gagaacaggt tttttgtcta caaatactag atttggacac tcaatcatgt
      601 attctgaacc agtgccgcca aaatcaaggt tgccaacttc ctcccatgtt tccacgttat
      661 tgtcatccgc tttgtaaagt ttgataaagc ctgatttttc taaattttgt gctccaacaa
      721 tcgcatacaa ttgtccttca taattaaaca tttgtggatc acgaaaatga tcagttacat
      781 cagcaggttt atgaataaga acattctcac ctttcgtaat gttgtagtct ttatccatcc
      841 atgctccgat ttgaagtgta tcacgttccc aattttcatt tcgaacgttt cctgtataga
      901 gtaagaatag attcccctct atttcatatg cagaaccgga ataagcgccg tggctatctc
      961 gttttgtgtc tggaagaagt tctgctcctg ttacatgaaa atgaactaaa tcatctgatt
     1021 cattgtgtac ccattctttc aacccatgag cagctccaaa tggccaattt tgataaaaaa
     1081 gcgtatattt tccgttaaaa aaagaaaaac cattaggatc gtttaacaat cctgttttag
     1141 gttcgatatg atagctagca cgccaaggtg atcgttttac attatcctta attttatcca
     1201 tctcatcttt agtccaatca gaataagcac gatatcgtat ttctcgaggt aagttcatat
     1261 tttactcctt caatttatct ttttacaact agtatggtaa acgttttccg ttgaaaaatc
     1321 aatcatttca tttaaaaaaa taaactatat gacaaacgtt tgacatattt ttaagatatg
     1381 ctaaaataaa tttgcatttc agtgaaccgt tttcacgtaa aaataaaaaa ggagtgattc
     1441 gaatgaatta cagtaaagta gcttccgacg taatacaagc ggtcggaaaa gataacctta
     1501 ttgcagctgc acactgcgct acacgtcttc gtctcgtact aaaagatgac acgaaagtga
     1561 atcaaaaagc tttagacgaa aatccagatg tgaaaagcac atttaaaatt aatggacaat
     1621 atcaagtgat tatcggtgca ggtgatgtta atttcgttta tgacgaactt atcaaaaaaa
     1681 ctggactttc ggaaatttca acagatgatt taaaacaaat tgctagtaaa gataaaaaat
     1741 tcaaccccat tatggcatta attaaattat tatcagatat ttttgttcca attatacctg
     1801 cccttgttgc aggtggttta ttaatggcat tacaaaactt tttaacagct gctggacttt
     1861 ttggaccaaa atcaattgaa gaaatgtatc ctgcgattga aggtatttcc tcaatgatcc
     1921 aactgatgtc cgctgcacca ttcatgttct taccaatttt agtaggtatt tcagctgcta
     1981 aacgttttgg agcaaaccaa tttcttggtg ctgcaattgg tatgatcatg acaacaccag
     2041 atcttggtgg ctcaactgaa tattggaata tttttggtta tcatgtttca caaacaaact
     2101 atgcttatca agttattcca gttttagcag ctgtttggat attatctata ttagaaaaat
     2161 tctttcataa aaaattgcca tcatctattg actttacatt cacgccatta ttatcagtaa
     2221 tgattactgg atttttgact ttcacagtta ttggccctgt aatgttattg ttgtctaatg
     2281 gaattacaga cattattgtt tggctatata acactactgg atttattgga atgggtattt
     2341 ttggaggaac atattcactt atagtcatga ctggtttaca tcaatcattt ccagctattg
     2401 aaactcaatt actttcagcg tggacaaatg gtatcggaca tggagacttt atctttgtag
     2461 ttgcatctat ggcaaacgtt gctcaaggtg ctgcaacatt tgccatttgg ttcttaacta
     2521 aaaattctaa gacaaaaagt cttgcttcct ctgcgggtgt ctcagcactt cttggaatta
     2581 cagagcctgc cttgttcgga gttaacttaa aataccgctt tccattcttt tgtgctttaa
     2641 ttggttcagg aattgctaca gcagtagcag gattacttaa agtagttgct gtttcacttg
     2701 gttcagcagg tttcttggga tttctttcaa ttaatgcaac atctattcca ttttacgtat
     2761 tgtgtgaatt gattagcttt gtagttgctt ttgcaattac ttacttctac ggtaaaacaa
     2821 aagctgctgg tatctttaaa gcagaagaaa ttgctgaaca aacaccagtt aattcttcaa
     2881 aagtagcaac aacagatcca atatctactg atgttgaatc aacacaaaca aaagtttcat
     2941 cagaaacaat tgttagtccc atttctggta aagtgattag tctttcttca gtaaatgatc
     3001 cagtcttttc atcagaagct atgggaaaag ggattgccat taaaccaaat ggaaacacgg
     3061 tccattctcc agtagatggt actattcaac tcacgtttga aactggacat gcttatggta
     3121 ttaaatcaaa taatggtgct gaagttctaa ttcatattgg tattgatact gtatcaatgg
     3181 gaggaaaagg atttacacaa aaagttacta ctaatcaaaa agttagaaaa ggcgatattc
     3241 ttggagcatt tgaccgttca atgattgaga aagaaggtct tgatgataca acaatgatta
     3301 ttgtgaccaa tacttcggat tattcagaag taaatatagt agctgaaaat acagtcactg
     3361 agggtactga tttacttgaa atcaaataaa tatatttccc tgagtaaaga tggaat
//
LOCUS       DACEQH010000121         3399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.129, whole genome shotgun sequence.
ACCESSION   DACEQH010000121 DACEQH010000000
VERSION     DACEQH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.129"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..175
                     /locus_tag="IW833_002693"
     CDS             <1..175
                     /locus_tag="IW833_002693"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308742.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRT"
     gene            191..277
                     /locus_tag="IW833_002694"
                     /pseudo
     CDS             191..277
                     /locus_tag="IW833_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873143.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
     gene            420..641
                     /locus_tag="IW833_002695"
     CDS             420..641
                     /locus_tag="IW833_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1308743.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="IW833_002696"
     CDS             657..1499
                     /locus_tag="IW833_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308744.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="IW833_002697"
     CDS             1562..3364
                     /locus_tag="IW833_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAQ1308745.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
BASE COUNT         1230 a          469 c          703 g          997 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta cctaagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactgctgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaata
//
LOCUS       DACEQH010000122         3378 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.130, whole genome shotgun sequence.
ACCESSION   DACEQH010000122 DACEQH010000000
VERSION     DACEQH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.130"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(53..2545)
                     /locus_tag="IW833_002698"
     CDS             complement(53..2545)
                     /locus_tag="IW833_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ1308746.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2552..3019)
                     /locus_tag="IW833_002699"
     CDS             complement(2552..3019)
                     /locus_tag="IW833_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ1308747.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(3155..3228)
                     /locus_tag="IW833_002700"
     tRNA            complement(3155..3228)
                     /locus_tag="IW833_002700"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3192..3194),aa:Arg,seq:acg)
     gene            complement(3286..3359)
                     /locus_tag="IW833_002701"
     tRNA            complement(3286..3359)
                     /locus_tag="IW833_002701"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3323..3325),aa:Pro,seq:tgg)
BASE COUNT          921 a          747 c          556 g         1154 t
ORIGIN      
        1 cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt aactataggg
       61 aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct tttgaagcac
      121 ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg ctcaatcgat
      181 cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca ggatcaaatc
      241 cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata tcttgttctt
      301 tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt tcatctttac
      361 tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt cggaaagctt
      421 tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta acatcttgga
      481 cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt gaagtcataa
      541 tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa tgaccatcat
      601 ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact tcatctaaga
      661 gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct tcatcatacc
      721 ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc ataaattctg
      781 acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa agtgctttgg
      841 ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca attggacgat
      901 caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct ttgacagcct
      961 catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag cgttcgcttt
     1021 cttttttctc caactgctgt agagggacac cagtccactc cgatacaact gtagcaacat
     1081 cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt gcagaaactt
     1141 tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca ttttcaaagt
     1201 cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct agtttgatca
     1261 cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct gattcatcga
     1321 tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca gaaagattga
     1381 ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca tatcttgtac
     1441 gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact tgaatgcgag
     1501 caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca tctagggtcg
     1561 ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata ttcgaagcat
     1621 caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca atgaataaga
     1681 ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct tcaaattcac
     1741 cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg cgcttttctt
     1801 gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca gcaatggctg
     1861 ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt cggctaagaa
     1921 tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc ttgttttctt
     1981 ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct tgcggtgttt
     2041 gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca gcagaatcac
     2101 cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg atacctaagt
     2161 tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc aaaatatgtt
     2221 ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa gcaaaaactt
     2281 gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg ataccaccat
     2341 aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc atttcacgta
     2401 atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca tgttctgaac
     2461 taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat actgcttggg
     2521 ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa cttaaacgtt
     2581 ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg aaattcaatg
     2641 tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt atttggtctt
     2701 cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca tcaaataact
     2761 gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca attcgtatat
     2821 aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta aaacgggtat
     2881 taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt tcaatccttc
     2941 gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca attaaatccg
     3001 aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct taattataac
     3061 gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt tagaatcaac
     3121 aaaaaaaaat acggaatata ctattccgta tcgacgcgcc caagaggagt cgaaccccta
     3181 accttttgat ccgtagtcaa acactctatc cagttgagct atgggcgcat attcaattta
     3241 aaaaaattat aaatcatttg aaaacaaaaa gcaagataaa aaaaatcggg aagacaggat
     3301 tcgaacctgc gaccccttgg tcccaaacca agtgctctac caagctgagc tacttcccgt
     3361 ttaaaaaata aaaaagac
//
LOCUS       DACEQH010000123         3344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.131, whole genome shotgun sequence.
ACCESSION   DACEQH010000123 DACEQH010000000
VERSION     DACEQH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.131"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IW833_002702"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IW833_002702"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ1308748.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="IW833_002703"
     CDS             complement(2261..2512)
                     /locus_tag="IW833_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ1308749.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="IW833_002704"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="IW833_002704"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ1308750.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          917 a          732 c          622 g         1073 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc tttt
//
LOCUS       DACEQH010000124         3308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.132, whole genome shotgun sequence.
ACCESSION   DACEQH010000124 DACEQH010000000
VERSION     DACEQH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.132"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            260..1214
                     /locus_tag="IW833_002705"
                     /pseudo
     CDS             260..1214
                     /locus_tag="IW833_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 105 protein"
     gene            1231..2255
                     /locus_tag="IW833_002706"
                     /pseudo
     CDS             1231..2255
                     /locus_tag="IW833_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            2261..2740
                     /locus_tag="IW833_002707"
     CDS             2261..2740
                     /locus_tag="IW833_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ1308751.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            2756..>3308
                     /locus_tag="IW833_002708"
     CDS             2756..>3308
                     /locus_tag="IW833_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ1308752.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVG"
BASE COUNT         1008 a          551 c          762 g          987 t
ORIGIN      
        1 ttcgttttca taaatctatt ataaaagaaa agactgtaga catcatccat aaaatggact
       61 ttgtctacag tctggaactt ggaaaacgtt gtttctccaa gttctttttt taatttccaa
      121 gtaactgaaa gttacttttg ttatttaaca tttaggtcaa ttttagacag tttttgttca
      181 aaaagcgata tttatagtgg acggtattac tgatatatta aggatataaa atgaaagcgt
      241 ttaaaaaggt ggcgtaaaga tgattattaa agaagagatt tttgtaccta aagaattagt
      301 gaaagaaaaa attgacctgc tcgttgaaaa cctgacaacg atcaaagacg ataatggtga
      361 atttttgttg ggctttgatg gactgaaagt agatgacaag agctggacag tgtggaattg
      421 gccacaaggt gtcggtctat atggtattta taaaaattac cagatgacaa aaaatcctcg
      481 cgcttatcaa gtggtcaatg aatggtttga ggatcgtatg gaagaaggcg ctccgcccaa
      541 aaacgtcaat acaatggcac cgctattgac tatggcttac ttgtatgaag acacgaaaga
      601 ttcacgctat ttgccttatt tagaacaatg ggcagaatgg gtcatgaatg acatgcctcg
      661 gacaaatgaa gacgggttac agcatgccac atatggaccg gaaaataaga atcagttgtg
      721 ggacgataca ttgatgatga cagtgcttcc tttggcaaaa atcgggaaac tgctggttga
      781 cgattgctat tccagagatc attgaaatct tagaattatc gaaagaagat gcattgcgca
      841 aattattgat cgaaacactt gaagcacaag tgcgtgcatt aaaaacttat cagtctgaaa
      901 gcggtttatg gcatacgttg ttggatgatc cttcttctta cgtagaatct tctgcaactg
      961 caggatttgc ttacggtatt ttgaaagcgg tccataaacg ttatttacca caagaatata
     1021 aagaagtcgc ttatcgtgct atccaaggat tgcttgaaca aatcgatgaa aaaggagaag
     1081 ttcaaaacgt ttcaatcggg acaggaatgg gggacagttt agatttttat cggaatatcg
     1141 gaatcacagc aatgccttat ggacagtcgt taacggtgct ttgtttgaca gaattgctcg
     1201 tctcttattg ttaaaagaaa ggaaacccat atgaaaaaaa catcactgct gtttgcagca
     1261 agttcgctta gcctaggtct gctaggtcta agcggctgcg gaaataattc tagtgtggca
     1321 actgtcgcag atgcagacgt aaatgagata gttaccttgc gctttgctta tgcaagcaac
     1381 agccagtaat tgatgcaatg aatgaatttg ggcgtttagt aaaagaaaaa acagatggtt
     1441 ccgtacagat ccagtatttc ccagatggac aactgggtgg agaacgtgaa ctgatcgaat
     1501 tgacacagtc aggagcagta gatttcacaa aagtaagtgc ctcagcgttg gaaagtttct
     1561 ctaaagatta ttcgattttt tccgttcctt atctattcaa tgatgaagaa catttcttta
     1621 aggtgatgga taatgaagaa ataatggagc caatctacca atcgaccaaa aatttaggat
     1681 ttaccggatt gacctattat gattctggtc agctcagctt ttatatggta gacggtccga
     1741 tccatacgcc ggaagatttg aagggaaaga agatccgtgt gatgcaaagc gaaaccgcta
     1801 tcaaaatggt agagctttta ggcggttcgc ccgtacctat gggaagtgat gaagtgtaca
     1861 cgtctcttca atccaatttg atcaacggat cagaaaacaa tgaatttgtc ttgtatacag
     1921 ctggacatgg cggggtagct aaatactatt cctatgatga gcatacacgt gtgccagata
     1981 tcatcatcat gaatgatgcc atcaaagaac agctaactga tgaacaggaa aaagccattg
     2041 aagaagcagc gaaagaatca acggtttttg aaattgaagc ttttgcaaaa gcaattgaag
     2101 aagaaaaagc tttagcacaa gaagaacatg gagtccaatt caatgatgta gataatacac
     2161 cgtttcgaga ggcagttgcc ccacttcatg atatattcaa aaacgatcct acctacaaag
     2221 acctttatca aaagatccaa gagcaaggag cgtagctgta atgaaaatga ttttaaacaa
     2281 ggtattagag atcctgggag ctgttttgct tgttgggatg gtcgctgttg ttttatggca
     2341 agtattttcc agaacggttt taggaaatcc aaatacggtc acggaagagc ttgttcgatt
     2401 cggtcttgtc tggtttgcca tgttgtcttc cgcgtatgtt gtgggacaaa aagcacgttt
     2461 agccgtaacg gttttaagtg aaaaattaac tggcaaaaaa agtaaaattt tagaagttgt
     2521 tgttcaaagt ttatttctgg tatttgctgc gatgatcatg gtgtatggcg gatggaatgc
     2581 tgtcacattg acaatggggc agatctcgcc ttctttaagt attcctatgg gatatgttta
     2641 tctgtcagta cctgtatcgg gggtactcat cattatctat agcctgatca atctaatcga
     2701 tacattaagc aacaagccag caattcaaga aaaaatataa aaatggagga ataaaatggc
     2761 aatagaagcc ggattagtgt tattttttgt atttgttgtg ttgcttgtat tagggatgcc
     2821 gattgctata agtattgcat tctcatcgat gatgacttta ttgttagtta taccgtttga
     2881 tgtttctgct tttagttcag ctcaaaaaat ggtgggaagt atcaatagtt tttcgctgat
     2941 cgctattccg tttttcgttc tctcgggcat tatcatgaat aatggaggga tagctaagaa
     3001 actagtggat tttgcgatgt tgttcgtcgg gcgcgtgccg ggggctttgg ctcatacgaa
     3061 tgtgttaggg aatgctttat ttggttccat ctctagctcg gccattgccg cttctaccgc
     3121 gattggtgga gtgttgatcc ctcagcaagt ggaagaagga tatgaccgga agtttgcgac
     3181 agcggtcaat atcgcgtcag ctccgacagg tatggtcatt cctccaagta ctgcttttat
     3241 catgtattca cttgttgctg gtggtgcttc gatctctgct ttgtttatgg gaggttatct
     3301 cgtaggcg
//
LOCUS       DACEQH010000125         3301 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.133, whole genome shotgun sequence.
ACCESSION   DACEQH010000125 DACEQH010000000
VERSION     DACEQH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.133"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            138..1106
                     /locus_tag="IW833_002709"
     CDS             138..1106
                     /locus_tag="IW833_002709"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ1308753.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(1423..1947)
                     /locus_tag="IW833_002710"
     CDS             complement(1423..1947)
                     /locus_tag="IW833_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ1308754.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            2234..>3301
                     /gene="scm"
                     /locus_tag="IW833_002711"
     CDS             2234..>3301
                     /gene="scm"
                     /locus_tag="IW833_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1308755.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSST"
BASE COUNT         1053 a          668 c          611 g          969 t
ORIGIN      
        1 aaaaatgaac gcccaccctc ttcttttttc tctttccttc tttcgttttt gctttatgta
       61 tcaaccgttg tttcttcgaa aactattttg ttctctcctt cttcctgata gaatggatgg
      121 gaaaggagct tgatgccatg acaacgaaag acaaggtact tcaattattg aaaaagacac
      181 cagattttct ttccggagaa aagttggcac agcaactgga agtttcccgg acaagtattt
      241 ggaaagccat caaagaattg gaaaaggcag gctatcgttt tgagcacggg cctacagggt
      301 atcgttacct gccctctgac gtactagatg caaaagaaat ccagcaagag ctacggtcgt
      361 ttattcctga tttgaaagta caaacgatgt tgacttctga atctacgatg aaagatgcca
      421 aactggcagc agctcaagga gattctgttt cgaagttatt tgttgctgat acacaagaac
      481 aagctcgtgg acgttttgga cggccttttt tcgctgaacc tggacgagga atctacatga
      541 gtctcttgct ccatccagac aaacggtttg atgaattgcc tcaatatacg atcatcgcag
      601 ccagtgcctg tgtgaaagcc attgaatctg taaccggtcg ttctgcttcg atcaaatggg
      661 tcaatgatat ctacatggat ggaaaaaaag tctgtgggat tttatctgaa gcagtcagtg
      721 atatggaaag cggcaggatc agtcatatca tcattgggat cgggcttaat ttttctgttc
      781 ctcaaaatca ttttccagct gatctccaag aaaaagccat ctctgtcttt cccgacggac
      841 aagctagtgt cacaaggaat cagctaatca gcaaaatctg gcaagaattg ttcacttatc
      901 tttctcaccc agaaaaagcg ttggacttct ataaagaaaa atcattcgta ttgggaaaaa
      961 ccgtgtcgtt caatcaaaaa ggaacgactt atgtcggtct tgcaaaagaa atcggtgatc
     1021 atggcgaact gatcgtccaa acaaatgaac agaagatgag tttgtcttct ggcgaaatca
     1081 gtctcacatc tatccgtacg caataattat cagatttcac aaaaggacac ctgatctcat
     1141 acagtgtcct tttttctgaa ttctcttcaa aagactctcc ttattctatc agctgacagt
     1201 ttctctatcc tctagaaaaa ggtattctaa aaatatctat aaagcatggt acactttctt
     1261 taaatgaatc aatgcaaaaa acaagagagt catcagttta cacgatcaaa agagatagca
     1321 ggccgcttcc tttttcaatc ttatagatag accatgggta acgataaaaa caaaaaagaa
     1381 cttgcgactt attttgccgt aagttctttt ttagctagtg ccttaatctc ttaatcccaa
     1441 caaacgtctc gcatagatca cctgaccaga gtgcatagca gcatcatcaa taatagaaac
     1501 gagacgcatt cctcgtgtga ctggtggtgt ccagttctcg tcaattactt catctagctc
     1561 ggaaggttcc agccttctta agtaagaaat agcaaaatca gcagctcctc gcaagtatcc
     1621 tagcagatct tctatttgat tagcttggat ctgctttgct tgttccagac tatgcttcca
     1681 atcttgttcg ttatctgcta tcgtcatcgg aaaccggctt tcccagtctt gactatgcca
     1741 aattggagaa gttcctgcta aatcagcaat ctggaaatct aattctcgag cggtatgcca
     1801 agccagccaa gtcattgatt tgatctgcgg ggctctatct tccactggaa aacgattggc
     1861 ctcctcaata gagacgcctt caaacaaacg ttcaaaccga tcgtatgccc gatccacact
     1921 ctcgcatagc atatctaaat aatccattta ttttcctcct ttttatttat cctacttact
     1981 ttttcctaat tctaggatac ctacttatat gttactacac acccttattt tttacacttt
     2041 aacagtcacc atcatcaata gtttggagat tgaaagaaaa agatagtgtc gaactatcaa
     2101 ttgaacaaga agcaacaata tgaacgtttc attaatattc tttaaataaa caatgatttt
     2161 tcaaacgaac agtttaagac taggagaaaa agagtataat aggaaaatgt ttgaaaggaa
     2221 aggaagggga aaaatgaaaa aaagggcaag tttactagtc ctagttgctt tgttgttcaa
     2281 tatcttttcg tatggtgtgg ccgtttcagc ggaaacatac attcctaaag accctgggaa
     2341 tatttataat gatctaactg gtaactatgg cttgttagga attgctagtc aattccacgt
     2401 atttgctaaa aataagacaa ctgtgaatgc acatacagac ggaaatatag caacacatga
     2461 acttgatgca aaaaataatt ttggtacaga gcttcatgaa ggtttattga accaagaaat
     2521 aaattatata caaaaaactg attctttcgc taattcttca ggcataccta caggtagcac
     2581 aagaatgaat aaatttgtcg ttggcagtaa cgtggatgta gcacttcaag atggtcatcc
     2641 tacagtcaat ggatccaggc ttgatcactt aactccagat gatttttata aagatcgtgc
     2701 tgggcatgaa tatattgatt tcgagcagga atttgctaaa ttgaatgtag cagcaaatga
     2761 tcttgcaacg atcactccag ctaaaacata tactgctgca gattttccag acatgaacaa
     2821 tcggacaatc gatttaactg atttatcaga taccgggttt ttattggtaa atatcgatgc
     2881 agaagtgtta acgatgaata cgccacttca aatcattaat ccaaatgatc aagtagttgt
     2941 atttaatgtc atcaacagtt cttctgcatt gaacgtacag tcaccaatca aatacaatga
     3001 tcgttcaaat catgaaacag aagatttttc ggatgcgaat atctcttgga attttggaaa
     3061 tgagatgact gatttgacaa tcagtgctcc ttttcaagga acaatccttg cgcctaatgc
     3121 tactatccgc attactcaaa accaagacgg aacgattata ggaacaaatg tgattttaga
     3181 tgcagctacc aatcgttggg atcctaacga aatatttatc agcgacaatg gtacagatac
     3241 gacagacaca agcgacagta cagatacatc taccacaagt gatagctcag attcatcaac
     3301 t
//
LOCUS       DACEQH010000126         3266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.134, whole genome shotgun sequence.
ACCESSION   DACEQH010000126 DACEQH010000000
VERSION     DACEQH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.134"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..251
                     /locus_tag="IW833_002712"
     CDS             <1..251
                     /locus_tag="IW833_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308756.1"
                     /translation="GEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNHRQF
                     IDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            265..954
                     /locus_tag="IW833_002713"
     CDS             265..954
                     /locus_tag="IW833_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308757.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1079..>3266
                     /locus_tag="IW833_002714"
     CDS             1079..>3266
                     /locus_tag="IW833_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ1308758.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVD"
BASE COUNT         1092 a          442 c          700 g         1032 t
ORIGIN      
        1 ccggggaaca gttagaagaa agtattccag gaattagtga ctcagataaa attgttgtct
       61 tgtctgctgg tgggcaatta atgggagatg gcaaatccga attagttagt tataaacaaa
      121 atcacagaca gtttattgat gttaatactg attccaacgc tatttcgatt aaggatctaa
      181 aagagctttt aaatagtaaa agaaatgaat taaagcagat aaaagaaact aattcagtgg
      241 aattagagta aaggaagatt cattatgaaa aagatttttg gtgtttctgt tgggctactt
      301 atgatgttaa catttggaaa tgcagtatat gctgctgaga aagaagttat ttcagatcca
      361 gagcagtacg cctcacgata ctttgctgat tttgaagaag aagataaagt acttgcattg
      421 ccagaaggtg gttttttaaa aggggaagct aaaattgtat ctatggataa tccagatata
      481 attcaaggtt catataattc agaaactgat cctgcttcag tttcagtgac agaagcaaaa
      541 aaaattgttg ctgaaaaatt caaaaatgat attgaaacta ctccaattat gcctcgagga
      601 gcatttccac ccgatgttgg tactagcgga caccttcttg tactagcagg aggaggaagc
      661 tataaatcac agccatttag tggtgcggga tggcgttttg gaggtatggg tgtaaaacca
      721 gctgatggta caggcaactt tttacgttgg aaatcctaca ttgacgatgg aagagtagga
      781 aatgtaaatc aagccaacgc aactaaatct ggttctattc aaggaatggc attgtatgct
      841 cgtgaaggat tcaaatactt tgcaccaaac gggcctggtt ggacaatgaa tataggtatt
      901 tattacactt ataatcctgt tgcaggtacg tattatgttg ttcaaaatat ttaataaaac
      961 agcacaaaga gatttctttg tgctgtttta ttatttttag ttgttgacaa acactaaaat
     1021 ataaaatata atcaacacat aaacgaacgt atgttcggca agaaagcagg ggatttttat
     1081 ggatgagatg gttttaagta ctcagaaatg gctaaacaag aaatacagta atgttacagg
     1141 gtttgataag gttccagaaa atgggcgaac agggtggcct acaatctatg gacttattga
     1201 aggactacaa gtagaacttg gtattactaa tctagtagct aattttggac ctaccactga
     1261 aaaaatgtac gataatcagg ttactcctaa atggggcaaa aatctaccta aaaatattgt
     1321 atttcttatt caaggtgcct tttggtgtaa ggggattaat ccaggtggct tcgatggggt
     1381 atatactcca tatttagata ctgctgtaaa agagttgcag actgatgcag ggtttaatgg
     1441 aagcgctgta actggagttt tggatgcgaa atgggccaaa gcgttatttg atatgagtgc
     1501 ttttgttctt gttcccggcg gagatagtaa aattagaaaa atgcagcaat ggttaaatgt
     1561 aaattattta gaatacacgg gaataatgcc ttgtgatggt atttatcaaa gaaattcaaa
     1621 tcaagcatta atttttgcat tacaagctga actaggatat tctcccagtg aagcaacagg
     1681 gtcttatggt aatgggacaa ctagtaaaac ttatcctgtg tctgaaggta actcgggaaa
     1741 ctatgtaaga ataattcaat atggattata tgttaatggt tattttgaag acggtacatt
     1801 cgatggtatt ttcactaaat atatgggttt agaagtactg gcattccgaa aatttatggt
     1861 attgcctgaa tatactgaaa tagcagatgt tgttgtaata aaaggactat tgtctagcgc
     1921 tggagatatt agaagatctg cagatggcgc agatacttct acgcaattaa ctagatctca
     1981 aatccaaaca ttggttgata atgatataaa aatagttggt agatatctta ctggtactgt
     2041 tggaattgga aaagatgaac gaaataagta tctaactaca gaggaattga ataatatttt
     2101 ttctaaaaat ttatctgtgt ttcctatcta tcaagatgga ggtgcagcat tagcttattt
     2161 tacttatgat cgtggcttat cagatggtaa gaaggctata gatgcagcaa aaaatctaaa
     2221 tataccatta acaacagtta tttattttgc tgttgatcta gacatgttag gggaagaaat
     2281 tcttgcatat gctggagaat attttaaggg ggtatctgct gtaatgtcat atagcgggta
     2341 tcaaacgggt gtttacggaa caagaaatgt ttgttctcaa ataatcaata acgggtatgc
     2401 ttcgtttgca tttgtttcag atatgtcgac tggttatagt gggaatttag gttttcctat
     2461 gcctagacaa tggtcttttg atcaatttgt tgaatttacc ataggaagtg gcaacggagc
     2521 tgttggtatc gatttaatcg ctacatcggg tagagatagc ggatttaatg agttatcaaa
     2581 tgatactaat aatgataatt acattgccaa atacaatcag aaggttataa atcagatggt
     2641 acgtgcgtat caatatctat cacaagctgg tcttgataat ccttggaacc ctcatttgac
     2701 attttataga tatgtaaatt attctggttt gtcatgggac atcatttctt ctcctgtaac
     2761 agagcatgat agaaaaattt atgatgaata taggaataaa ttaactaata gcgagggcct
     2821 gtataattat tttatagatc ctaatactgg ttcaataata ggtttgcctc acttaattgt
     2881 gacgttacaa tcacagatgt ttctagccga cacaatcaat gatagtgttt ctgactttgc
     2941 aggttggtta ggagatttaa tgacttgctg gggtgaagtt aagaaattag gaatacaaat
     3001 ggagcaaggt gtatttgcat tagtaggtac ggctgcaggt ggaacattta gtctagaaga
     3061 tttataccaa gatgttgacg cggtaaatgt ttatacctta gcaaaaagaa attctgattc
     3121 aacagcttta tcggctttaa aaacgtacta ttgtgataag ggtgctattg atagatttaa
     3181 tcaatttgtt aagaacagat ttggttcttc agataaaatc gcttcagtaa caaatagttt
     3241 attagaaaat gacgaaacag ttgata
//
LOCUS       DACEQH010000127         3221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.135, whole genome shotgun sequence.
ACCESSION   DACEQH010000127 DACEQH010000000
VERSION     DACEQH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.135"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            50..1438
                     /locus_tag="IW833_002715"
     CDS             50..1438
                     /locus_tag="IW833_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ1308759.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1776..>3221
                     /locus_tag="IW833_002716"
     CDS             1776..>3221
                     /locus_tag="IW833_002716"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1308760.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEEE"
BASE COUNT         1185 a          505 c          583 g          948 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct gcctatggcg
     2761 tattagcccc taaatcaaaa gcggactttg ctttcttatt acatggctac tatcatttga
     2821 aagatacggg cgtcatggcc attgttttgc cacatggggt gctctttaga gggggcgcag
     2881 aagggaagat acgcaaagcc ttgttagaaa atggtgccat tgacaccgtc attggcttac
     2941 ctgctaacat cttctttaat acgtccattc caacgaccgt aattatcttg aagaaagacc
     3001 gtgcgaaaaa agatgtgctc tttattgatg cgtcacaaga ttttgaaaaa ataaaaacgc
     3061 aaaatacgct acgagattac catattgacg ctattttaga ggcctataaa acccgtacaa
     3121 ttaaagacaa gtatgcttat gtcgcagaat ttgatgaaat tgtggagaat gattacaact
     3181 taaacattcc ccgttacgtg gatacctttg aagaagaaga a
//
LOCUS       DACEQH010000128         3024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.137, whole genome shotgun sequence.
ACCESSION   DACEQH010000128 DACEQH010000000
VERSION     DACEQH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.137"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..881
                     /locus_tag="IW833_002717"
     CDS             6..881
                     /locus_tag="IW833_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1308761.1"
                     /translation="MDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYEGNNQLE
                     NRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGRKIVLLP
                     FDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFMINIIAM
                     FALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQLAPKGI
                     RVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLASNKASYV
                     TAQIYGVTGGEAINL"
     gene            919..1482
                     /gene="amaP"
                     /locus_tag="IW833_002718"
     CDS             919..1482
                     /gene="amaP"
                     /locus_tag="IW833_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1308762.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            1496..1684
                     /locus_tag="IW833_002719"
     CDS             1496..1684
                     /locus_tag="IW833_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1308763.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            1697..2599
                     /locus_tag="IW833_002720"
     CDS             1697..2599
                     /locus_tag="IW833_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1308764.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            2631..2873
                     /locus_tag="IW833_002721"
     CDS             2631..2873
                     /locus_tag="IW833_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1308765.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
BASE COUNT         1080 a          553 c          640 g          751 t
ORIGIN      
        1 aattaatcga cccgcgaaac ttgtaccata cggagaaatt tcctaaacaa gatcaggata
       61 caccagcttt acagcagaat atgattcctg ttcctgattg tggagaagaa tcctatgaag
      121 gcaataatca actagaaaac cgccgtgtac taatcactgg tggagattca gggatcggtc
      181 gtgcagcagc gattgcattt gctagagaag gggcagatat cgctcttcaa ttcttcccag
      241 gagaagaaaa agatgcagag gaagtagcaa aatacgtgaa agttgcagga cgtaagattg
      301 tcttactacc ttttgatctt cgtgacgaac aagcacctaa agaaatcata gataaagccg
      361 tgagtgaact tggcgggcta gatacgatgg tactaaacgc aggacagcaa atttcttgtc
      421 agtctattgc tgagttacca cttgagcaag tcaaagacac atttatgata aacatcatcg
      481 ctatgtttgc tttagtgaaa gaagctgttc cgcatatccc agcaggagga gcaatcgtaa
      541 caacaacttc tgttcaagca ttcaatccaa gcgaacattt attagattac gcagcaacaa
      601 aagcttcaat cgctaacttt acgatcggtt tagcaaaaca gcttgcacct aaaggaatcc
      661 gtgtaaatgg tgtagcacct ggtccaatct ggacaccatt acaactagac gaaggacaac
      721 cttctgattc gatccctgaa tttggtcagc aaagtttact agaacgtgca ggtcagccag
      781 gagaattagc accagtttat gttttcttag catcgaataa agcaagttat gttacagccc
      841 aaatttacgg agtaactggt ggagaagcga tcaacttata agaagttgat cgtttcaccc
      901 ctatgaggag gtgcgaaaat gaataaaggt gtaaaaacga tcggagtgat cggaacatta
      961 cttttgctat ctgtgctttt gtttgcgata ctattaagtg caacctatcc attgcctttt
     1021 cagctagaga agtttcgatt cttttccatc actaattcat acgtgcaaca atatgtattt
     1081 tggcttgccg ttgcatttgc tattttgaca attatcacgt tacttgtttt cctgttttat
     1141 ccaaaaacaa tcggaaactt tctactgaaa catggtgatg gggagttgac gttggataaa
     1201 aaagcaattg aaggaatggt tcgttctcac ttgcatgaag atgaattcat ccattcacca
     1261 aaagtaaatg tcaaagcaac gaaaaacaag atcgccgtca atgtaaaagg ggagttgaaa
     1321 cgaacttcag cactcattgg taaaacagga acattgatga aagaaatcga aaatgaagta
     1381 caacacatat tgggaacaaa agagccagtg aaagtatcgg tgaaatacac aggctttgaa
     1441 gaaccagcta aacgaagcaa tgaccgcaaa cgagtagaat agggggggac caaaaatgaa
     1501 agaactattt tcgacataca agctccctat catctttggg gtacttggat tgatattagc
     1561 cgttttatgg atcaccgtgg gctttttcaa gaccttattg atcttgatca tggcagcgat
     1621 cggcgtcgct atcggttttt atctgaaaca aacgaaaatc ttagatcact attttaaatc
     1681 ttaggaggaa acatccatga ataacaataa caaaaataat aacaataaaa tgaacaacca
     1741 aaaaccagtg tctgcacatc cagacccaac gacaccaaac caatcaaatg gtgtgaacga
     1801 tgaaaagaac aaaacagcac cagtatctgc acatccagat ccagtagtcc ctaacgttca
     1861 acaaggagta gaagagaaga aacatgatgc agaaaagcat gcacctcatc atccaggaac
     1921 tccagaatct aaagttccag aacaacatga tgcaatcgat ccttctaaac ctgtggaaga
     1981 gcaaaaacat ccttctgacc atgcgaaagc acaagaaaaa cttagttctg accacacaaa
     2041 cggacaagga aaacttggtt ctgatcatac aaataaccaa ggaaagcatg attctgaaca
     2101 ggaagattca atgcgcggtg agctgaatta tgaagataaa gttgtgcaaa aaatcatcgg
     2161 aatcgcaatt gaacaagtag acggtttatt atctgcaaac ggtggtttct tctctaatgt
     2221 ggcaggaaaa cttgtgaata cagataatgt aacggctggg atcgaaacag aagtcggtaa
     2281 aaaacaagta gcagttgata tggatgtcat tgtggaatat ggcaaagata tcgaaaaaat
     2341 ctttgaagaa atgcaagaag taatcggcaa agaagtaaaa aatatgacac atttagaagt
     2401 gatcgaagtc aacgctaacg tagtggacat caaaacaaaa gaagaatttg aaaaagatca
     2461 agaaacggtg caagacaaag taactgacgc agcgaaaaaa accggtgaat ttgcttcgaa
     2521 tcaaacagat aaagtaaaat cagcagctgg aagcggcgca caaaaagtaa aagaaaatac
     2581 agaaccacgg gttcagtaat aaggaagtaa aacattagga ggaacgaaac atgggattta
     2641 tttggtcact tatcgtcgga ggaattatcg gagcaattgc aggagcaatc acgaataaag
     2701 gttcttcgat ggggattatt gcaaatgtcg tagcaggttt agttggatca gcacttggtc
     2761 aagcattatt aggttcttgg ggaccaagta ttgcaggaat ggcaatcgtg ccatcattga
     2821 ttggtgccat cattttagta ctaatcgtat cattctttgc tcgaaaactg taaaaagact
     2881 tttaaaaaga ggtcgtgaaa aagctgttct tcatcaagta ataagaacag ccaaacaaaa
     2941 atagcttctc atatttttca tttggttggg catattatcg aagatgcaag cttttgaaca
     3001 ctgtttatac ggaataagag gctg
//
LOCUS       DACEQH010000129         2954 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.138, whole genome shotgun sequence.
ACCESSION   DACEQH010000129 DACEQH010000000
VERSION     DACEQH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.138"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            204..1172
                     /locus_tag="IW833_002722"
     CDS             204..1172
                     /locus_tag="IW833_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1308766.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1165..2196
                     /gene="vanA"
                     /locus_tag="IW833_002723"
     CDS             1165..2196
                     /gene="vanA"
                     /locus_tag="IW833_002723"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1308767.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2202..2810
                     /locus_tag="IW833_002724"
     CDS             2202..2810
                     /locus_tag="IW833_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1308768.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          879 a          561 c          715 g          799 t
ORIGIN      
        1 aattttttag gaaaatctca aggttatctt tactttttct taggaaatta acaatttaat
       61 attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga gccgttttcg
      121 ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt tggtgccttt
      181 cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat ggatgtgagc
      241 aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg gcaacgataa
      301 ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg
      361 tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga gccggtgtga
      421 aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct gctaagagaa
      481 tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat tatactatga
      541 tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg
      601 atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt ggtgtggtgg
      661 gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga tgtaaagtgt
      721 tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat gagttgctgc
      781 aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac tatattatca
      841 gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact gggcgcggtc
      901 cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg ggcggtgccg
      961 cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc caaaaaccaa
     1021 ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc acaccgcata
     1081 cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt
     1141 tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact gtttgggggt
     1201 tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc taacattaat
     1261 aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg gaaaatgtgc
     1321 gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact ctcgccggat
     1381 aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat
     1441 gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca aggtctgttt
     1501 gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat ttgtatggac
     1561 aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc cttttgggtt
     1621 attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt tgttaagccg
     1681 gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga attggactac
     1741 gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc
     1801 tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagctgttgg cgaggtggac
     1861 caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc
     1921 tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg aggacggata
     1981 caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc ccgtgtggat
     2041 atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct gcccggtttc
     2101 acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact tcccgaactg
     2161 attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata ggatttactt
     2221 ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact tgggataatt
     2281 tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca tacgagttgg
     2341 ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga ttgcttctat
     2401 gggacggtta ccgtcctaat cgtgctgtaa actgttttat gcaatgggct gcacagccgg
     2461 aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag atgatttcaa
     2521 aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat cttacgcttt
     2581 atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt atggatgaac
     2641 gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc
     2701 gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg cactatgtat
     2761 taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa acttttaacc
     2821 gttgcacgga caaactatat aagctaactc tttcggcagg aaacccgacg tatgtaactg
     2881 gttcttaggg aatttatata tagtagatag tattgaagag gttctgttgc aaagttttaa
     2941 ataaagaata aaat
//
LOCUS       DACEQH010000130         2821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.139, whole genome shotgun sequence.
ACCESSION   DACEQH010000130 DACEQH010000000
VERSION     DACEQH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.139"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            411..1529
                     /locus_tag="IW833_002725"
     CDS             411..1529
                     /locus_tag="IW833_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308769.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1830..2150
                     /locus_tag="IW833_002726"
     CDS             1830..2150
                     /locus_tag="IW833_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308770.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            2602..2802
                     /locus_tag="IW833_002727"
     CDS             2602..2802
                     /locus_tag="IW833_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1308771.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
BASE COUNT          954 a          453 c          573 g          841 t
ORIGIN      
        1 gagagtttca atgtttctga aaaaacttta ggataagaac gcgcgttatg gaagtttagc
       61 ggtcgttttt acgtactttt acaggaatag gccgtgtttc cgcatctaat aagcgggaag
      121 cgctgaaagc aaataaactg ataatactta ataaactgaa aatcataatt aaatgcataa
      181 gatccacctc ttttattctt tttcttagtt tatcagcatt tcatgaagaa tagttgaaga
      241 ctggggctaa ataattgagg aaattatgaa gataacttaa aaaaatagct aaatttaaaa
      301 ccgtagaaac cgttttattt ttttagatac gttttattgt atggaaaaat agtgttgtgg
      361 tagactttag atagaaacaa aggaatgatt tttttaggag gggtacgttt atggcaaaaa
      421 aggataaaga attattgaca caattgactt cagaaggcgc taaaggacca ttcgtcacga
      481 tcatgctgaa tacgcatgtc gcccatcaag atgtggaaaa agatcagatc aaattcaaaa
      541 attttgcgaa agaagcaaag aaacgttttg agaaaaaata ttccgaacat gactggtctc
      601 cgttccaaga aaaaattgaa aatttgttag gagatccttc gttttggcgt agtgcaacta
      661 cgagtgtagc ggtcattata actgctgacc agacctatat ccatcgtttg agtatcccgg
      721 tagacgatca atactatgta agtgatcttc cttatttact ggctattatc aaaaatgggc
      781 agttcaacta ttcttattat ttattgggat tgaaccgcga ctccatgaaa ttgtatgagg
      841 tcagcaacaa acaagtaaaa gaagtatctc taccagaagg ggcaccggtt gatgtcgtta
      901 ctgcattggg atctgaactg acaggtggcg gtttgaactt ctcttctaat ggaggaggcg
      961 gagcccgaga tggagtaact ttccatggcg taaatccaaa agatgaagaa gtagagatcg
     1021 attgggtcaa ctattaccaa gctgttgata cgtacttaaa ggattctttc gaaaatcctg
     1081 aacaatggcc gatttattta tttgcattac cagaaaatca gacgatgttc aaaaaaattg
     1141 ctaaaaatcc ctattattgt gcagacgttg cgatttctgc ttctcctgcg cagctatcct
     1201 ttagagatat cgaagaaggg gcgaagaagc tgtcaagtga actcgcagct caagaatcaa
     1261 aagaatacaa taaactctta gataagaaat tcatcgatca gttagttgat atcgttccag
     1321 ctgcgaaaga aggaaaagtg tcacatttat ttatcgctac gtcaaactta gctgatggtt
     1381 tcggtgaaga tcctgaaaca gaatatgatc ggcgtcaagt gttgaatgca ttagcagatg
     1441 atgtgatcat taatggcggg aaagtgttta ttttggatca aaaagatgcg ccagatgaaa
     1501 agagtctgac agcgattctt cgttattaaa agaggttgtg aaatcagtgt tttgacctga
     1561 gtattaagga agaggttgtg acagaagtgg tcagcttcaa gaaataagaa gagattgaca
     1621 aaaattattt ctcaaatttt tgtgaaattt cggcttattt ccgaagaagc tacttctgga
     1681 acaccgttta aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca
     1741 aaaaacatca tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt
     1801 gggaaaaact tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt
     1861 ttctatccct aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta
     1921 ttacaggcac acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga
     1981 ttcctgaaac agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga
     2041 cctgtgatta tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta
     2101 ctcatactca gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa
     2161 aaaagaaaaa ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg tcactgtcat
     2221 aggctatcag aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac
     2281 cgttgaaaca gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca
     2341 tcagagcaaa aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcgttttt
     2401 tcactttatg taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct
     2461 tttttgtaaa cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct
     2521 tgtttcaaat aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga
     2581 tacacaagga ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa
     2641 ggatttggat tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc
     2701 caaggcgacg gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa
     2761 ggccaacgcg gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa
     2821 t
//
LOCUS       DACEQH010000131         2778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.140, whole genome shotgun sequence.
ACCESSION   DACEQH010000131 DACEQH010000000
VERSION     DACEQH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.140"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..197
                     /locus_tag="IW833_002728"
     CDS             <1..197
                     /locus_tag="IW833_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308772.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IW833_002729"
     CDS             314..811
                     /locus_tag="IW833_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1308773.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="IW833_002730"
     CDS             898..1290
                     /locus_tag="IW833_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1308774.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..>2778
                     /locus_tag="IW833_002731"
     CDS             1274..>2778
                     /locus_tag="IW833_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1308775.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          518 c          596 g          825 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagc
//
LOCUS       DACEQH010000132         2704 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.141, whole genome shotgun sequence.
ACCESSION   DACEQH010000132 DACEQH010000000
VERSION     DACEQH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.141"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(33..506)
                     /gene="dfrF"
                     /locus_tag="IW833_002732"
     CDS             complement(33..506)
                     /gene="dfrF"
                     /locus_tag="IW833_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ1308776.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(925..1143)
                     /locus_tag="IW833_002733"
                     /pseudo
     CDS             complement(925..1143)
                     /locus_tag="IW833_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1174..2388)
                     /locus_tag="IW833_002734"
     CDS             complement(1174..2388)
                     /locus_tag="IW833_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1308777.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT          852 a          590 c          413 g          849 t
ORIGIN      
        1 tgaaaacacc ctaaatccaa aatttactca attcattttt tccttacata aaatgttctc
       61 gtatatttca cttcctcacc aagtgtttcc cctatcaaaa cttcaaaatc attgatatca
      121 aattctggaa agaatgtatc tccatcttca atatttaaat ctacttctgt gatatacatt
      181 ttatctacta tttgcaaagc ttccttaaat agtccatatc caccagatat gtatacatct
      241 cgtcctttag ccaataataa tgcatcttct aatgatttaa ctgaaactaa attatctcct
      301 tgatactctg ttgtggtgga aacaacaata ttcattctat taggcaacgg atgaccgatt
      361 tcttcataag actttcgccc cataataacc acattacccg ttgttaactc tctaaattgc
      421 ttttgttctc cctttatttt ccatggtata ttaccattct tgcctataac attattcttt
      481 gacctcgcaa caatcaaacc tatcatctat ttcatcctcc ataatatgat gtcgtacctg
      541 tgattccatc aagtgcacca catcttaaca aactcaaaat tatctcgaac taaccagctc
      601 gacaaattcc ttttttgtct tcatctgcat tatatcatat tgttataagc attgccattt
      661 cttttctgct tttgaaaagt tacttatcag cctatgtttt gtaaagggtg atattcagca
      721 tttctcacgc aacaagccct tgacaaacca tagactaata agttttaagg taatcaagca
      781 gaaatgaaat agtctgctgt ttcacggcaa caaaaaagag ataccaacac acttatgtag
      841 tatctcttag tctagtagca atattctttt gtttgcaagc aggtaaacat gggctgtttc
      901 ttaatatctt tttgtttggg aaacaccttt ttcccatcct ttcaccgttg ccatctctgc
      961 tgcgcacaat gccacaggga taaaccatag caaaccgcct aataacaaga aaaatactaa
     1021 gctgaagcct gaggtcgcga atgtgggata ttcatataat gaaacgacca ttgagatggt
     1081 catagccaaa aagccaaaga acgttaattt ttttgcagga attttcgttt tgctttgttg
     1141 catgaatctt cctcttttct atttgaattt gtttcaattg agtttgttgc caaagaagaa
     1201 aaagtccaat ccttcttctt gttcttcttt tgtagtagtg atcgttgtaa agtgcatcgc
     1261 cgattttaaa atcactgttg tcagcacata ggcatacacg gcagtaatga tgacaccaat
     1321 cacttgaatc cctaagaagt accagctgct tggattcgtt agtaaatggc tgttagatgc
     1381 aaagatccct atcagaatcg tacctgtgaa tccgcccatg ccatgaacac cccacacatc
     1441 aagtgcatca tcccattgtt tctttttacg aaaatcaaca gcagcatgac agacgatacc
     1501 cgcaatgatc ccaatcagca tggcactttt tgcttctaca taaccggcac aaggcgtaat
     1561 ggctgccaat ccagcaacgg atcctgttag tacgtcgaca aaatcaaagc catttccttt
     1621 tacttttgca atgatcagcc aagtaatcat cgcaaatgca agagcgatga atgttgaacc
     1681 aaatgcattg actgcttgtt catctgctcg aagtgcacca ccggaattaa aaccgaacca
     1741 gccaaaccaa agcagtcctg taccgatagc agctgccata agactgctat gctgacttct
     1801 ttctcctaaa ggaatctttc tttgaccaag tgtgaggata catgctaaag agccgaatcc
     1861 agctgtcgtg tggatgactg ctccaccagc gaaatctcta aatcccatct gatccaaaaa
     1921 tccgccaccc catatccagt ggcaaacagg gaaataaatc agcagattcc aaaaaataac
     1981 aagtataata tagcctttta aattcaatct ccctgcaaaa gctcctgtca tcaaaggaac
     2041 ggtaatcacg ataaacatca gttggtaaag aaagaacatt aaaaatggaa tcgttgcacc
     2101 atgaaacatg ttggggaagc ttgtaacatg tctcatcaaa aagaaatctg ccgggtttcc
     2161 aataacaccg ccgatatccc gtccaaaggc caatccgaat ccaccaaata cccacataat
     2221 cgttgttatc ccaacggaga gaaaagattg agacatgatc gttagtatat tattcttccg
     2281 tgctaatccg ccgtaaaaaa aagctagacc aggagtcatc aggcaaacca ttgctgtaca
     2341 taaaatcatg aatgctgtat ctcctgtatt gataggtaat gtattcattt ttttcgcctc
     2401 ctttctacga ttacgagtat aaaagaaggt gtagttgttt ttttcttctg tttttttgaa
     2461 ttaatacttg catttataaa agacagacaa ttaataaaag cttgatgtgt tgtgctcttt
     2521 ttcgtttttc acacaaataa agacaaaaaa taaacggtct tctttttata gaaaaccgtt
     2581 tattttcgtc tttttttatt tttaaagata ttttttcata aggaataaag aatacttcat
     2641 ataaaatata taaaaaagaa ccgcagcgag tgtcgctacg gttctcaaat ttgttcttca
     2701 tttc
//
LOCUS       DACEQH010000133         2654 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.142, whole genome shotgun sequence.
ACCESSION   DACEQH010000133 DACEQH010000000
VERSION     DACEQH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.142"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            712..2571
                     /gene="ltrA"
                     /locus_tag="IW833_002735"
     CDS             712..2571
                     /gene="ltrA"
                     /locus_tag="IW833_002735"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1308778.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
BASE COUNT         1027 a          421 c          509 g          697 t
ORIGIN      
        1 aaaaggttag gcacttaaaa gaagaaaaca taagtttcct tttaaggaga actgatattt
       61 tgagcagtgg aatgatgggg taacgccctg aaacgctccc ttaatactcc gactggctgt
      121 ttaacatata gctgttagat ggtcagaagc tcggtgaagt cggctgaaag ttaccgtaaa
      181 tatgataaat tcatatacga aagttgttca gaggtgaatg atgtaaccta taggtcggag
      241 gtctataaaa tactcacggt gagaatgtaa gtaatactac tgacgaactt gcgaatgaaa
      301 gaatctatac acggactgta tagaaatgta caggattcta ccgtatcaac ggaacagaat
      361 gtatgttgtg gtttagtaag attattggtt atgaacaacc atgcaatgtt acaggcattg
      421 gaaatataca ggttcaaagc aagcacctaa aagtatatat aaagataaaa atatcgaaac
      481 atgggaagct ggcaatgtgg aggaaactat attccattga aacatttgtg acgaagaata
      541 ataagcacat ttatgccagt gaaagtggtg gcacagtacc aatgaggata tagaaatata
      601 tcggaggaat agccactagt caaaagaaat cgttaattaa atgatgtatt tcatagaatc
      661 gagtaagaca aaataaacac tacaacctaa gtaaacaaag gtagtggtgc tatgaataaa
      721 gtaaaaacaa ataaaacact tcgtcatagt gaatactatg gcatgacaca aatatttgat
      781 gaactgtatg taaaaagtga taaaaaagaa gtttttacag agctcatgac aattattatg
      841 agtgatgaaa atattcgact agcatacaga aatatcaaga ataacaaggg cagttataca
      901 gcgagtaccg ataagaaaag tattcgagac attaaaacac tgtcagaaga tacctttcta
      961 actgaggtta aagttctttt tcgctgttac aaaccacgag cagttagacg tgtagaaatt
     1021 cccaaaccaa atggaaaaac acgccctctg ggcattccat caatctggga taggttaata
     1081 caacagtgta ttttacaagt cttagaaccg atatgcgaag ctaaatttca caattatagt
     1141 tatggatttc gaccaaaccg ttccacacat catgcgattg ctacctgcaa tcgactggca
     1201 aataggggga agctacactt tgtagtagat gttgatatta aaggattctt tgataatgtc
     1261 aatcactcta aacttttgcg tcaaatgtgg acgattggaa tccgtgataa atggctatta
     1321 ggtattattc gatccatgct aaatgctcct atagtcctac caaccggtaa agtcgaacac
     1381 cctaaaaaag gtgtacctca aggtggtgtc ctatcccctt tactatctaa tatcatattg
     1441 aatgaattag attggtgggt tgcttcacaa tggcaaaata ccacgattga gacagaaaag
     1501 attatggata ggcgagataa aggcaaaggc attgataaga ctaatcagta tcgggcatta
     1561 agacgaacaa acttaaaaga aatatacatt gttcggtatg ctgacgactt caaaatcttt
     1621 tgtagaaaac gaagtcatgc agataaaata tttcacgcaa cgaaaatgtg gcttaaagaa
     1681 cggttatcac ttgaaattag cgaagaaaaa tccaaaattg tcaatctcaa aaaaggtaaa
     1741 tcagaatatc taggatttga attaaagcta atcaagaaag gtaaaaaata cgtagtcgaa
     1801 agtcatatga gcaaaaaatc aatgacaaga gttaaaatcc aattaaagaa acaattacgt
     1861 aaagttgcca gaccgaagaa tcattgcgaa caagcaaaag aaattggatt atataattct
     1921 atggtgattg gaatccatca atattatgga attgcaacct gtgtaaatct cgattgcagt
     1981 aaaatagcct atacactaaa accgttcatc aatcacaaac tgccgacaaa gaaacatgga
     2041 aagatattaa atacatttat caaatcgaag tatggaaaaa gtaaagaaat gcgatggttg
     2101 aataatgtgc caattgtacc gctcaactat gttcagttta gacttgctgt ccccctatca
     2161 gagggaactt gcaagtacac tgaaagtgga cgaagcaaga tacacagtaa attaaaatta
     2221 gacctagccc ttttacttca catgatgaga aatagtaatt atgaaagaag tattgaattt
     2281 gtggataaca gaatatctaa atattctgct caaaagggaa aatgtgctat cacgggtaaa
     2341 tttctcgaat acgaagaaat ccattgccac catatcgtac cagtaaaaca acagggtaca
     2401 gacaaataca gcaacctcat tattattcac aaaaatgttc acgcattagt ccatgctatc
     2461 gaagaaaaaa tcatacacaa atacctgaat gtattaaatc taaccaatga acaaatagaa
     2521 aaactaaatg aattacgtgt caaagctggt aatagtgttt taacagttta acttgaaaaa
     2581 tatatcatac ttaacattct tttgatggaa cgccgtatga agtgaaagtt tcatgtacgg
     2641 tgtgaagcgg ggga
//
LOCUS       DACEQH010000134         2623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.143, whole genome shotgun sequence.
ACCESSION   DACEQH010000134 DACEQH010000000
VERSION     DACEQH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.143"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            36..197
                     /locus_tag="IW833_002736"
     CDS             36..197
                     /locus_tag="IW833_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308779.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            210..713
                     /locus_tag="IW833_002737"
     CDS             210..713
                     /locus_tag="IW833_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1308780.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            773..1162
                     /locus_tag="IW833_002738"
     CDS             773..1162
                     /locus_tag="IW833_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1308781.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1149..>2623
                     /locus_tag="IW833_002739"
     CDS             1149..>2623
                     /locus_tag="IW833_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1308782.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT          779 a          434 c          603 g          807 t
ORIGIN      
        1 aatggttttt aaccaatgcc caatccttag tcttactggc aattgtggtc attggtttgt
       61 ttttaggatt taagcgggag ttttccaaac tgattggctt tttggtgatt gctttgattg
      121 cggtcggatt ggtctttaat gcttctgggg tgaaggatgt cttactcagt ttgtttaacc
      181 gaattgtcgg tgcgtaaggg ggaagcacga tggaacagat gcgtgtttat attgcgaacc
      241 tcgggaaata taacgaaggt gaattggtgg gggcgtggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacgaatt gggttaaatg acgaatacga ggaatatgca attcatgatt
      361 atgaacttcc ctttgagatt gatgagtata cgccaattga ggaaattaat cggttgtgtg
      421 gcttagcgga ggaacttgaa ggaacgccag ttggtgaagt tgcttctgag attcaacatg
      481 ctttcttcaa ttcttttgag gagatggtag agcatgtgga tgatattatc tattatccag
      541 attgcgatga tatgagtgat gtagcatatt tcttgattgt agaagctggt gattttggag
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtaga gacttggaga
      661 ttggaggcaa ttatttggta acgagtcgag gtgtttttga gtatccatat taacaaaatt
      721 attgaagggt agttgatttc taaactatcc ttctttttaa aggaatgatt tgatgaagaa
      781 aataaaaagc tacaccagta tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt
      841 tcaactgcct tttcctgtga cttttaacca gatggcttgg tttgttttat ccttgctgtt
      901 tgtcatcgta tttgcccatg tgcctccact atctatgata gagggggcct ttttaaaata
      961 cttaggtata ccggtggcag ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa
     1021 accattggga tttttacgat cttttattag ctatcactta cgatttaagg tcactttcgc
     1081 aggaaagaaa gtcaaagaac agaagaaacg atgggacgaa cccattactc tggtaaggag
     1141 tgtgaactat gtacccgatt aagtatattg aaaataatct tgtctttaat caagagggag
     1201 aatgttttgc ctactatgag ttaatacctt acaattactc ctttttgtca cctgaacaga
     1261 aatatcaagt ccacgataac tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc
     1321 atgccttaca aattgccaca gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg
     1381 aaattaccgg gcgattaaca gaagtagcga aacaacgaat tgatttacag acagaggcat
     1441 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc ttcaaactga
     1501 ttgcgacaga tgaagaggtc aatctaaaga atctcaagaa atctttcttc tccggctttc
     1561 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
     1621 aagaaattcg ccgatatacc aagcttgaaa aactaatgga aagtaaattg gctcgaagat
     1681 ttaaagtcag acgagtcaca ccgagtgatc tgatgtattt gattgaacat atctatggcg
     1741 aaaagggtac accatttgaa gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg
     1801 aaactttggt gaaacgttac gacttattac gtcccagtcg ttgtttgatt gaagaaaagc
     1861 cccggtactt atgcatggaa catgagaatc atgaatcata tgtagcgtac ttaacaatta
     1921 atacaattgt gggggaaatg gagtttccct cctcagaact tttctattac cagcaacaac
     1981 aatttacatt cccaattgac actagtatga atgtggagat tgtgacgaac aaaaaggcac
     2041 ttgctaccgt tcgcaataag aaaaaggaat taaaagactt ggacaaccat gcctatcaat
     2101 ctgacaatga aacaaattcc aatgtcttag atgcattgga ttccgtagat gaattggaaa
     2161 ctaccttgga tcagtccaaa gaatccatgt acaaactgag ctatgtggtt cgagtaagtg
     2221 ccgaatcagt agatgaacta aagcgccggt gtgatgaagt gctagatttt tatgatgata
     2281 ccaatgtaaa actagtccgg ccttttggcg atatgatggg gctacatgaa gaattcttac
     2341 cttctagtaa gcgatacatg aatgattata tccagtatgt tacgtctgat tttctcgccg
     2401 gacttggttt tggcgccact cagatgttag gggagttgga aggcatttac tttggttata
     2461 acgtagatac ggggcgcaat gtttacctga aaccggcatt ggcctcacaa ggggtcaagg
     2521 gttcagttac caatgcgcta gcagcggcat tcctcggttc actcggtggc ggaaagtcct
     2581 ttagtaacaa tctcttagtt tattacgccg tcctttttgg tgg
//
LOCUS       DACEQH010000135         2622 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.144, whole genome shotgun sequence.
ACCESSION   DACEQH010000135 DACEQH010000000
VERSION     DACEQH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.144"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="IW833_002740"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="IW833_002740"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1308783.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          940 a          458 c          581 g          643 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg ga
//
LOCUS       DACEQH010000136         2485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.145, whole genome shotgun sequence.
ACCESSION   DACEQH010000136 DACEQH010000000
VERSION     DACEQH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.145"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1016..1306)
                     /locus_tag="IW833_002741"
     CDS             complement(1016..1306)
                     /locus_tag="IW833_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308784.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1514..2205)
                     /locus_tag="IW833_002742"
                     /pseudo
     CDS             complement(1514..2205)
                     /locus_tag="IW833_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          735 a          459 c          348 g          943 t
ORIGIN      
        1 acatacttgc aaagtaatac tacaattcct aacataatcg tagaatttaa taatttccat
       61 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
      121 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      181 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      241 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      301 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      361 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      421 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      481 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      541 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      601 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      661 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      721 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      781 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      841 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      901 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      961 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
     1021 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1081 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1141 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1201 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1261 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1321 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1381 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1441 tactaaaaaa aagaggcagc agtatagatt actgttgctt ctttactaat ttaggtttac
     1501 ttgttaagaa agattattta cgcaaatgtt cttcaacaat ttgctgagat ttatcagctg
     1561 tatcttccac ttcatctttt gctttttcta acttttgaat atattgtttc aagcttgagt
     1621 tttcagcagt tgcttcgtct aatttctttt gtgtatctgt taactgattt ctacatttgt
     1681 tttttcttgt gtcagtgttg cgatttgttg attcaaacca tttacagtat tttgtaattc
     1741 tgaaagtttt gcttgtgctt cttgtagtct tgctgtccaa tctgcattca gatgattgat
     1801 ttcatcattt ttttggttga tctcatctag tttgacattg atttcatttt gtttgctttg
     1861 tagttgtgca tccttgctga ctaattgact ttctaagttt tgtttttctg ctgccgattg
     1921 atttttttgg ttttctaatt catttacttt tgtttggtat tcttggacat ctgcctttga
     1981 atcttgcaat gtctgttctt tttgtgctag tcgagcggta agatctgtaa tcgcttgctc
     2041 tttatctgtc aatgtatgat caagctgtgt cagattctca ttgattgtat caatatctgt
     2101 ctctccaccc cacaaaataa tttcatttgc gtaccgcgtg cctgtgtagc caattagaca
     2161 tagaaatgca acgacaatcc caccagtaat aaatttcttt ttcatttgca tagctcctta
     2221 atccaatact tttacatgct aaaagtaagt gatctcaaac acaatgtcaa gcgtttttaa
     2281 attacatatg taattttaat ataaatacta tcaaatactc atttaatcct ccttttttat
     2341 ctatttaaaa aatctttttt atagagaaaa atgcaggtta atactttctt aaataaatat
     2401 ttatggtgaa caaaaacaaa aaagcaagtt ttattaaacg taaccctttt catttcatag
     2461 attactgtat ttattacaaa aaacg
//
LOCUS       DACEQH010000137         2423 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.146, whole genome shotgun sequence.
ACCESSION   DACEQH010000137 DACEQH010000000
VERSION     DACEQH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.146"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..290
                     /locus_tag="IW833_002743"
     CDS             <1..290
                     /locus_tag="IW833_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308785.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(803..876)
                     /locus_tag="IW833_002744"
     tRNA            complement(803..876)
                     /locus_tag="IW833_002744"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(840..842),aa:Ile,seq:cat)
     gene            complement(1065..2084)
                     /locus_tag="IW833_002745"
     CDS             complement(1065..2084)
                     /locus_tag="IW833_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1308786.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(2095..2301)
                     /locus_tag="IW833_002746"
     CDS             complement(2095..2301)
                     /locus_tag="IW833_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1308787.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          740 a          466 c          405 g          812 t
ORIGIN      
        1 atgagacaca attatggttt aaatttgacc cttcgaatag atttatcaaa gatttttata
       61 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
      121 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaaggatg
      181 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
      241 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa agtaggctac
      301 ctaaaaaggt agcccggaac gaattttatc accatacttg tgaaaggaga tattttttaa
      361 gttagtatta agattgtgta atatgatgat atctatattt tatagtatca gtgctataaa
      421 atcaaaaata agtcactaat taactaccac tccaattgta agcctttttt ctcacttttt
      481 ttcaaaaata tggtatgcct tttaatagct tcaaatataa aagagtttaa agcgtaacac
      541 acttatgggg aagtggcttg gggtgcgctt taaactcttc tttattatca tctcacaatt
      601 taacccaaat gtctttctat ttaaaaatca aagtaaaact tttcaaatat acagaaatat
      661 aactatgtga aacatccttt cattaatcca taaaaggata cataaaaaag ccactcattt
      721 gagtggcaat gaagaaaagc tttagcttgt ataatactct tcaaaaaatt ctaacacaga
      781 acgattcaaa tggctacgtt aatgtaccct gtaggactcg aacctacgac cggacggtta
      841 tgagccgtct gctctgacca actgagctaa gggtactggt tgttgccaca taaagccata
      901 aacaatcaac cagtagaatg tgtggcaaca aacctgttat cgcatagctt ggagtgtgac
      961 tattatgggt gatagtgaag atatgcgata acatcactat tttatcgaat gatttttata
     1021 gttgtcaata tagttatgta atgctcctca acgaggagct atttttatcg tttaggaata
     1081 tttaaatacc aacgtttgtc atggaaatct tgcgcaccgc ctttagtgtt tccctctgga
     1141 tcattcgttg cccgcatcat gacatagact ttcttattag ggaagttacg catgttaaaa
     1201 gatacatgat agccaacatt tccagaagta ttataagctt gatttacatc tgatctataa
     1261 attccatcag ctcttactcg agctaattct ttcccagtat tgtaatccat aatgaagata
     1321 tactcgtatt tatagttagc aatgtgccat ccagctacat gcaaatttgc attttcgatt
     1381 tctccaaact gatcaatgtg ggcgtaattc gttccatctg tcagtgtagg attagctgca
     1441 ccagctcgtg ttggatcaat gacaggcttg ttttcagaag ttgttggatt ttcatcggta
     1501 aatccatgag ctaaatcata tgctaatttt tctttactta cgcccatttc agaaagataa
     1561 ccgtaaggat ctgtatgatc gccccagatg ttttgtgtta cccataaatg cgatttgatt
     1621 cccggttggt tataaggagt gtccaacgtt aatggaatac catatttcat tgctgaatct
     1681 ctagccaatt caacgtatgc cttgtagttt ttctcaaacg ttgctttatc atgtgtgtgt
     1741 tgtaactcaa tctgcacagg actgttggca ttagcatacg aaccagcacc gtactgtaca
     1801 taaccaggtt gaccgacttg ataaacaatt ccgccgtctc ccacaatata agcagtataa
     1861 gcgctagtcc atgaacgttg catatactgc gcttcattgc gtcctgttgc tgtttcatta
     1921 gccgtttcat gcagtaaaat atactgatta tttgctactt gtgagctacc ttcatttgcg
     1981 cccaaattaa attcattgtt gatagtatag gcaaatccgt taattggcaa taaaaaaaga
     2041 gccgttaaca ggctcatcgc agtaatagta attttctttt tcatatctat cctcctattt
     2101 atctgatgaa attttcttat acgttcgatt tgatacacct aaaatactcc ctagaaatgc
     2161 tccaaaacct gtaataatca tcaccgttat atctgtgtac cgccagttaa caccttttcc
     2221 gacaagaccg acaaaagtcg ctaaagcggg gataactact atagcgatcc atttcaaaat
     2281 atcaaaggtt ctattgttca tactatccac ctcccttcat tggttgggct actttctttt
     2341 tcaatcgctt gttttcttct atcaatattt ctctttctga aagcaaggca tcaatttgcc
     2401 cgtccagttg ttcaatttta tct
//
LOCUS       DACEQH010000138         2401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.147, whole genome shotgun sequence.
ACCESSION   DACEQH010000138 DACEQH010000000
VERSION     DACEQH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.147"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            206..2068
                     /locus_tag="IW833_002747"
     CDS             206..2068
                     /locus_tag="IW833_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1308788.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..2400
                     /locus_tag="IW833_002748"
     CDS             2068..2400
                     /locus_tag="IW833_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1308789.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          716 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataattaa
     2401 t
//
LOCUS       DACEQH010000139         2237 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.148, whole genome shotgun sequence.
ACCESSION   DACEQH010000139 DACEQH010000000
VERSION     DACEQH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.148"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            83..1129
                     /gene="gmd"
                     /locus_tag="IW833_002749"
     CDS             83..1129
                     /gene="gmd"
                     /locus_tag="IW833_002749"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAQ1308790.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
     gene            1209..2222
                     /locus_tag="IW833_002750"
     CDS             1209..2222
                     /locus_tag="IW833_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308791.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
BASE COUNT          599 a          517 c          546 g          575 t
ORIGIN      
        1 ttatcgtgta ttcttgaaaa gtattagaaa ttacgagcca tttatatggt cttatcactt
       61 acataggaaa ggataatgta tcatgaaaaa agcgctgatt actggtatta ctggtcagga
      121 cggttcctat ctggcagatt tcctgctaga gaagggctac gaagttcacg gtatcactcg
      181 ccgtgcttct atttccaata ccgctcgcat tgaccatttg atgggcaaga tcacccttca
      241 tgacggtgac ctgtccgact catcttctct gattcgtatc atcagcattg ttcagcctga
      301 tgaaatctat aacctcgctg cacagagcca tgttcaggtt tcttttgatg ttcctgagta
      361 ctccggcgat gttgatgctt tgggcgttat gcgtattctg gaagcttgcc gtattctggg
      421 cctgacgaag aagactaaga tttatcaggc atctacttct gagctgtatg gcaaggttga
      481 agaagttcct cagcgtgaaa ccactccgtt tcatccttac agcccttatg ctgttgcaaa
      541 gcagtatggc ttctggatca ctaaggaata tcgtgaagct tatggcatgt tcgctgtcaa
      601 cggtattctg ttcaaccatg aatccgaacg tcgcggtgag aatttcgtca cccgtaagat
      661 caccctcgct gccggccgca ttgcagaagg cctgcaggac catctggaac tgggcaacat
      721 ggactccctg cgtgactggg gctatgcaaa ggactatgtt gagtgcatgt ggatgattct
      781 ccagcatgag actcccgagg acttcgtgat cgctaccggc gagcagcaca ctgttcgtga
      841 cttcaccgag aaggcattcg cagcaaatgg catgaccatt cgttgggaag gcactggcat
      901 tgatgaaaag ggctacgatg ttgaaaccgg caagatgctc gtttgtgtaa atccacagtg
      961 gttccgtccg actgacgttg acaacctgtg gggcgacccg actaaggcaa agaccgttct
     1021 cggttggaat ccgcagaaga cctcttacga ggaactggtt aacatcatgg caaagcatga
     1081 ccgtgaattg gcaaagcgcg aagctgcaat gaagaacgca gtcaagtaag ctgacattga
     1141 ataaacatat ctgtttctat ctttccagtc acggcttcgg acatatcgcg cggaatctgc
     1201 ccattattat ggcaacactc tgccgatgcg aagcgaagat tacactggtt tgcggcgcaa
     1261 atcatctgga ttttgcaaag cgcaatttgg agcaggaact gcccaaagag ctgatagacc
     1321 gcgtgcgcta tcgtccaata aacacagata tcggtctgat taaccgtgag ggcaccctac
     1381 tggtggacac gggtaagctg accgagatct gtgaatattt tttggcaaag gttccttctt
     1441 tggcaaagac agaagccgaa tggctcaagc gagaagctgt cagcgcagtg ctttgcgata
     1501 tgccaatctg ggcaatggat gcctgcgata tggcaggagt acctttcctg tatgtaggaa
     1561 acttcacttg gacagagctg tatcgcgaat ttttacccga acatatttgg aaagaatatg
     1621 ccaagcgtta cggtaaaatt cagcacacca tgctttatgc gctaaataac gaagaaatgt
     1681 tggaatttct ccaaaacagc gaaaaaacac cgacttccgt tgtggcgcgt ccgattcata
     1741 acgacgaagt gcaacgcatt aaatcacagc atgaaaaacc gcctgtattt gtcgcactcg
     1801 gtatgtccgc gcggttcaaa cagccaattg atgtcagtca cgcgccgtat gatttcatta
     1861 caacgctagg tgttccgctt gtgggagaaa acgtaaccgt tatttctaac agcaccccca
     1921 atacacagga ctatatagcg gcggcagatt acgttattac aaaggctgga tggggaactg
     1981 ttgcggagtg tattctcgca cagcgtccga tggcactgtt cgcccgcgat acagttttgg
     2041 aagatagaac aactatttgt aaattgcaaa aactgggtct ggcaatttcc gtcacaccgg
     2101 aaaacctcca ccgcgtagag cgggtgattg aggacctcaa acagctagac acctcaaatt
     2161 ttgatacatt ttatgatgct gcgagcgaaa tcgcagacaa attgatttct ctcatgcaat
     2221 gagagatgct ctaaatt
//
LOCUS       DACEQH010000140         2213 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.149, whole genome shotgun sequence.
ACCESSION   DACEQH010000140 DACEQH010000000
VERSION     DACEQH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.149"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            2..184
                     /locus_tag="IW833_002751"
     CDS             2..184
                     /locus_tag="IW833_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308792.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            168..353
                     /locus_tag="IW833_002752"
     CDS             168..353
                     /locus_tag="IW833_002752"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAQ1308793.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            596..2116
                     /locus_tag="IW833_002753"
     CDS             596..2116
                     /locus_tag="IW833_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1308794.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
BASE COUNT          757 a          380 c          458 g          618 t
ORIGIN      
        1 aatgaataaa aaagatattg taccagttca attttcaagt gcggctcaaa aatcgcaaat
       61 tgacaccaag ccaaaactag cttgcagaat caaatcggaa gataaggaaa tatttattta
      121 taatggaatg gataaatatc ttttgtatac cttgttaaaa gagttgaatg ccaatgctca
      181 ttgatgcaac tgcattgaaa catattttta tcgtttgtgg aaaaacagat cttcgaaaag
      241 gaattgatgg actagcagct ttcgttatag aagaatatga ccttgatgtt tacgatcaag
      301 cgcttttctt attttgcggt acgaagaaag atcgcttcaa ggctctttac taggatcaaa
      361 atggatttgt attaatgtac aaaagatttg aaagtgggta ccttcaatgg ccgcgttcca
      421 gaaaagaagt cttactgact ggcctttcaa tcgaagaaaa atgtacgcta aaagaaacga
      481 aaaaaggtgc gatatattag acgaaaacct tgttaaatca aggattttgt ctgtttttcg
      541 ttgtcataaa atggtataat agatgtaaga aaatcgctag aatggtggtg taaaaatgac
      601 aaaaatggaa tctgaacttc ttgtacaaat caagcaatta acacagttgc ttgaaatcca
      661 gcaacaggaa aatactcttc ttagagaaca aattgctgaa atgaatcgac gcttgtttgg
      721 acgaaaaaaa gaaacaccac ccgtagatgg gcaaatcgat ttattagacg actcaacttt
      781 taatgaacca gagcacactg gacaagaaag ccaggaaccc attacggtat cttcttttaa
      841 acgcagaaaa cgcaagggac tcaaagcact ttcattagaa ggtctgcctg aagttcctat
      901 tcattatgaa ttaaaaggtg ccgattgttt gtgtgaagac tgtggtcata ctttacatga
      961 tatcggtgcg acaaggctta gagaagagcc attgtatgtt cctgcccata tagaaaaaca
     1021 agtcatctac caacatgcct atcattgcag agaatgtgaa gcattaggag aaacaaaaat
     1081 caaaaaagcg cctgttccta aaccgttgat caataatagt ctaggttcct cttcaattgt
     1141 gacacagagt atttgggaaa aatttgttaa aaaggtgccg gcatatcgac aagaaaaaga
     1201 atggcatgca cttggtttcc ctcttgatcg taaaaaaatc actaattggc atatcaaggt
     1261 aagtgaaatg gttttagagc caatctacaa ttgccttcat caagaattaa gcaatcagga
     1321 ggtcttacac gcggatgaga caagctatcg ggtattgaat actcataaag aaaagacctt
     1381 ttactggtta tatgcttcag gtaaaagcga agaaaaacaa gttgtgctct acgagcacgc
     1441 aaattcgcgt tccgttgatg agccaaaaag ttttttagaa tcatttagcg gtttcttgca
     1501 tactgatggt tacgcggcct atggaaagct gccggtaaac aatgtttatt gttgggcgca
     1561 tgttcgcaga aagttttttg aagcattggg taaacaaccg tccccaaaaa gtcatgccaa
     1621 aataggatta gacttctgtg acaagatgtt tcgaatcgaa cggaccttta ataagcttag
     1681 cgcagaagaa aggtttgtag cgaggaagga aacgttgcag cctgtgatga ctgaattttt
     1741 tcattggtgt gagagttttg cttatttgcc aaaatcgcag cttggaaagg cggttcaata
     1801 cgcagtcaat cataaagacg gacttcagat tgtgttgctt gatggacgct tggaactttc
     1861 aaataaccga gcagagcgtg ccatcaaaga gttagtgatc ggtcgaaaaa actggctatt
     1921 ttctaaaagc ttaaaaggcg ctagatcaaa tggaattata ttgagtatta tccagacagc
     1981 tgttgcgaat gggttaaaca tccgcaaata tttaaatcat ctttttacag agattcccaa
     2041 tctgtcttcg atgacacctg aagcgctacg cgcttatttg ccatggaatc agcagataca
     2101 agaaatctgt aaataatata agccacaaat aacctgattt gagtgtatca aatcgggcta
     2161 tttgtgttta ggtaccattc tatgacacgc ttacgctcaa aactactgaa tta
//
LOCUS       DACEQH010000141         2189 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.150, whole genome shotgun sequence.
ACCESSION   DACEQH010000141 DACEQH010000000
VERSION     DACEQH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.150"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(325..528)
                     /locus_tag="IW833_002754"
     CDS             complement(325..528)
                     /locus_tag="IW833_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308795.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(987..1952)
                     /locus_tag="IW833_002755"
     CDS             complement(987..1952)
                     /locus_tag="IW833_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1308796.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          629 a          506 c          346 g          708 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggctttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cgcgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgagggt tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cttaaacatt gacgtaaatt tactctcact tatctgactt ttttactcgg tatcactccg
      721 agtaagccgt catattcatt gggataatct cgtctgcttc gctctaattt tccagagtga
      781 ggggtaactc cttcccccga attaccccaa agagtgacac tgggatatcc ctaaatttat
      841 tataaaaagc caagtctctt tgcgcaactg ttttatccaa gtcggcattt gcggattcgt
      901 tagcgttttt tcgctcttgc tgcaaacgca cgacccacca tgccgacttg tgctgaaaat
      961 aaaaaagtgg acaaaaattc atccactcat tcttccgtca cgtccaaccc caaaagccac
     1021 gtatccaatt cttttttcaa aacatctgcc gattccgaaa atgtctctcg taaatgatta
     1081 actaactcat cactttggct ttcttttttc aacaaatttc gatatttata ttttgtatga
     1141 cgagccaagc cagcccataa attttgatct gaaatcaatc ctgcaatcat aattctatca
     1201 gtatgctttg tatcagtcgg caaaaaatga ggacttgcga aactgtctaa actttcatga
     1261 accatcgcat accaatcagt agcttttcca cgccgtaact gcaattccag tcgccaccaa
     1321 gtttttattt ccggtggtac aattttccgt ttggtttctt gttccaattt tttgttgtac
     1381 atccttattt gtcgctcact ggcacgacta ccccaatatg ccgtttccag cttcccagac
     1441 cgcccataga ttggtttgaa tgaaacaggg tcaaccacac gatattgcgt aataaaatca
     1501 tcaggcacat cgataatgtc gcaagcaata tcagcacgtg agaaatgtgg ttctaaaaat
     1561 aaatcatgga taaagttctt catcgaaccc gccaaaaatt ggctaatttt atttgggtta
     1621 aagtcaattc gcccttgccc ttcttgatat ttcaaaattt caatgtaagc aatattctca
     1681 tcatgcttat ccacaatttg ccaaccattt agaccaacag atttaacata accattattt
     1741 tcattcagtc gcattaactg ttcaaagtct aaaatcaata catcattttg cgtgtgataa
     1801 taaatattct ctttgagttt tccaacaatg gtaattcggt caattgacca ctgtatttta
     1861 aggggggtgg tcaaagtttg cacacttaac cgcctgttag atagggctgt taaattttcc
     1921 cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa gaaaaagaga
     1981 cttttggaaa gaatttccag agttcacgta accctaaaaa ttcatatcca aaaatctcta
     2041 gcttgaagaa acctaaaaat attcgtataa tgaacctgag ttacggagct aggtgaagta
     2101 gtttccgtaa atagtcgaga gtgttagcgc actgctcggc ttttattttg ctcttttttc
     2161 tcgttttctt tgattatagc actcctcct
//
LOCUS       DACEQH010000142         2186 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.151, whole genome shotgun sequence.
ACCESSION   DACEQH010000142 DACEQH010000000
VERSION     DACEQH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.151"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            74..340
                     /locus_tag="IW833_002756"
     CDS             74..340
                     /locus_tag="IW833_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308797.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            398..910
                     /locus_tag="IW833_002757"
     CDS             398..910
                     /locus_tag="IW833_002757"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1308798.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            907..1944
                     /locus_tag="IW833_002758"
     CDS             907..1944
                     /locus_tag="IW833_002758"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1308799.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            2104..2186
                     /locus_tag="IW833_002759"
                     /pseudo
     CDS             2104..2186
                     /locus_tag="IW833_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          788 a          416 c          451 g          531 t
ORIGIN      
        1 attcttcaag atatatattc gcaaatgtaa ttggaaaagc gacttcttaa acaaaattaa
       61 ggagtcgctt tttatggcaa aaataacgaa aaatgagcaa gaaaatttgg tggaaaatta
      121 cacaaaaaaa ccggcgtact ctaatagccg gttagacgca catttcgtgt gcacttctaa
      181 tccgaaattg agctttgacg ctatgacgat agtcggaaac cttaataaaa acagcgctaa
      241 aaagctatcc gattttatga gtttagatcc acaaattaga ctttgggata tacttcaaac
      301 aaaatttaaa gctaaagcac tacaagaccc cctatgttag gctagttgtc gtataggata
      361 ataagttata ataaagacta tacggaggat gattaccatg acaaataaaa acaatgaacg
      421 ttactctacc gaactgaaag cgacagccat caagaaaatg atgccacccg aaaatagggg
      481 gattcgccaa ttaagtcaaa ctttgggcat tccagaagcg actctttgtt cttggcgtaa
      541 aaaagctaga attcaaggga atactacacc cggaacgaaa aaaaatgtcg aaaaatggtc
      601 aagtgaagat aaatttttaa ttgtgatgga atcattctca atgaaccaag tagaacttgc
      661 ggaatattgc cgaaagaagg gcttatttaa agaacaaatt gaggcttgga agaaaacgtg
      721 cttatctgcg aatgaacagg aagaaaatcg aacgagagaa cttgcaactg aactaaaaga
      781 agagaaaaaa caagcgcgtc aattagagaa agaattacgt cataaggaaa aagcattagc
      841 ggaagccgct gctctcttat tactaagaaa aaaagcacac gcgatttggg gggaccaaga
      901 ggaagaatga tttcatcttc agatcgcaaa caagctgttg aacttattca agaagccaat
      961 cgcaatgggg cccgtctcac ctatgcctgt aatgagctga atattagcgt tcgaacctac
     1021 gaacgatgga cgcgtgaagg tacgatagcg catgatcaac gccctttggc gaaacgtcct
     1081 gtacccaaaa ataaactgac ggatcaagaa cgcgaaaaaa taatagaaac cgtttcaaaa
     1141 aaagaattta tgaatttgcc acctagccaa attgtaccaa aacttgcgga ttgctcgatt
     1201 tatattgcat cagagtctag cttctatcgt gtattgcgag aaaaaaatat gcaacatcac
     1261 cgtggccgaa gtcaagtttc acaaaaacgc ataccgccaa gtcaccttgc gacgaagcca
     1321 aatgaagttt ggacttggga tattacttgg ctaaaaggtc caataaaagg actcttttac
     1381 cggttatatc tcatcattga tttatttagc cgtaaaatgg taggctggga gatttgggaa
     1441 tcagaagaag ctcgtcatgc cgaaaaactt attaaacaga cggtgcttaa ggaacgtatc
     1501 caaggacagc cgttggtttt acactccgat aatggcagcc ctatgaaagc cgcgactttt
     1561 caagtcttac tcgaaaaatt aggaattcaa agttcttatt caagaccacg agtgagtaat
     1621 gacaatcctt attcggaagc ggctttccga acgttaaaat atcgaccaga atatcccaat
     1681 aacggcttta aaacaataga tgatgctcga aaatggacga aagagtttgt cgattggtat
     1741 aacgattctc atttgcatag tgggattcaa tttgtgtctc ctgcagcgtg tcatgaagga
     1801 cgacatctcg ctattttaga gaaacgaaag caggtttacg aggacgctaa aaagcgccat
     1861 cctgaacgct ggtcaaaagg tgttcgaaat tggactccac atcaatcggt ggcgttgaat
     1921 cctttaaaag aaacagaaag ttaacaggag cataatcttt caagattatc aaataaccct
     1981 cgtcacccaa taggggaaaa agttattctt cccctattgg ataacgaggc caaccgacca
     2041 tagggaggtt ctgctttttg ataggtggaa aataaaaata cgacaactat attgacaaac
     2101 accgaccatc gtccagtaaa gagacgcaat aaattctatc gaagtttacg cactgcctct
     2161 acaacgatta aaggcatgga agccat
//
LOCUS       DACEQH010000143         2125 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.152, whole genome shotgun sequence.
ACCESSION   DACEQH010000143 DACEQH010000000
VERSION     DACEQH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.152"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            57..884
                     /gene="panB"
                     /locus_tag="IW833_002760"
     CDS             57..884
                     /gene="panB"
                     /locus_tag="IW833_002760"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAQ1308800.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            899..1747
                     /locus_tag="IW833_002761"
     CDS             899..1747
                     /locus_tag="IW833_002761"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAQ1308801.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            1757..>2125
                     /locus_tag="IW833_002762"
     CDS             1757..>2125
                     /locus_tag="IW833_002762"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAQ1308802.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKH"
BASE COUNT          839 a          203 c          460 g          623 t
ORIGIN      
        1 aatagagatt atagtgaatt agaaaagtat ttaggagaga gttaggagag aaatagatga
       61 aaaatacagc agttacattt aaagaatcta aattaagaaa tgaaaaatta acaatgctta
      121 cagcttatga ctattcaaca gcaaaaataa ttgatgaagc tgggataaat ggaatattag
      181 taggagattc tcttggtatg gtatgtctag gacatgaaga tacactttca gtaacaatgg
      241 aagacatgat tcatcatact agagcagtta caagaggagc taagaacact ttagttgttg
      301 cagatatgcc atttatgtct tatcaaactt cagtatatga ttcagtagtt aatgcaggaa
      361 gattaataaa agaaggacga gctcaggttg ttaaattaga aggagggata gaagtatgcg
      421 ataaaataga ggctatagta aaggcttcta tacctgttat ggcgcatata ggtttaacac
      481 ctcaatctgt taatgcattt ggaggattta aggtacaagg aaaggacaag gaagctgcta
      541 aagaattaat aagagctgct aaagctgtag aaaaagcagg agcattcgct gtagtgttag
      601 aatgtgttcc aactaagctt gcagagttaa tttctaaaga gatatcaata cctacaattg
      661 gtattggagc aggtgcaggg tgtgatggac aaattcttgt atatcaagat atgttgggga
      721 tgtatagtga ttttacccct aaatttgtga agaaatatgc taatttatca gaagagatga
      781 ataaagcatt tacaaaatat atagaggaag taaaagatgg agtttttcca ggtccagaac
      841 atgggtttaa aataagtgat gatgttttag aaaaattata ttaaaagggt caagaaatat
      901 ggttatattt aaaacagtga atgatgttag aagtcaagta aaagagtgga aaaaacaagg
      961 cttaaaggta ggattagttc ctactatggg atatttacat gaaggtcatg aaagtttaat
     1021 tagaaaagca tcaaaagaaa atgataaagt tgttgtaagt atctttgtaa atccaactca
     1081 gtttgggaaa aatgaagatt taggtagtta tccaagagac ttagaaagag atatagaagt
     1141 atgcacaaga ggtagagcaa cagcaatatt taatcctgaa gttgaagaga tgtattgtga
     1201 caatgcaagt acttttgtta atataactgg attaacagaa ggtttatgtg gagctagtag
     1261 gccaatacat tttagagggg tttgcactgt tgtaagtaaa ttatttaaca taataccagc
     1321 agatagagct tattttggag aaaaggatgc acaacagtta gctgtaatta aaagaatggt
     1381 tagggacctt aatattgata tagacgttgt agggtgtcca attataaggg aagaggatgg
     1441 actagctaaa agttctagaa acacatactt aagtttagag gaaagatctt cagctactat
     1501 tttaaataaa tctttaactt tagctaagga agctttaaat aatggagaga gagacagctt
     1561 aaaaataatt gagataataa gtaaaaatat aaatacttat aatttggcaa agattgatta
     1621 tgtagaagtt gttgattctt tatcattaca aagagtaaat tatattgaaa aatcagtttt
     1681 agttgcaata gcagtattta taggaaaaac aagattaata gataatttta catttgaatt
     1741 acaatagggg gctataatga aagttacaat gttaaaagga aaaatacata gagctacagt
     1801 tgaacaagct gagttagatt atgtaggaag tattactgtt gatatgaatt tattagaagc
     1861 agcaggaata tatgaatatg aaaaagtaca aatagttgat gttaataatg gaaatagatt
     1921 tgaaacttat acaatagctg gagagagagg aacagggatg atttgcttga atggagctgc
     1981 agctagatgt gtatcaactg gagataaaat aattttaatg gcatattgtg aattaaatga
     2041 aaatgaggtt aaggatcata gacctaaggt tgtttttgtc gatgataata ataaagttga
     2101 aagagtaaca tcatatgaaa aacat
//
LOCUS       DACEQH010000144         1947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.153, whole genome shotgun sequence.
ACCESSION   DACEQH010000144 DACEQH010000000
VERSION     DACEQH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.153"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            180..482
                     /locus_tag="IW833_002763"
     CDS             180..482
                     /locus_tag="IW833_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308803.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(998..1071)
                     /locus_tag="IW833_002764"
     tRNA            complement(998..1071)
                     /locus_tag="IW833_002764"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1035..1037),aa:Ile,seq:cat)
     gene            complement(1626..1940)
                     /locus_tag="IW833_002765"
     CDS             complement(1626..1940)
                     /locus_tag="IW833_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308804.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          708 a          289 c          301 g          649 t
ORIGIN      
        1 tttataaggg tttgagcatt ttttattttc gcagattttc gttaaatgaa tgaaaatggc
       61 attcaaaata gcattcaaaa agaacacttt ttaatatata tgttccatcc atattatcaa
      121 tgatattcct actttacaaa aagaacatta gttcgtatac tctttttgag gtgaacatta
      181 tgttgatgga aggaaaaaca caattatggt ttaaatttga tccttcgaat agatttgtca
      241 aagattttta taaggtgtgg gattcagaag ttttcttttt agcaatcgaa gatagcttat
      301 taatcaatct ctattactct aataagaact actttaaaat tccggcaaca aaaaccagaa
      361 tgaagaaaga tgtgtatttt ttgtttgata tcgtgactga tgtgccagac gcacggagcg
      421 atcatcggcg ttatgactat ttgaagtata ctttcgttga tccagaaagg tataaagatt
      481 aaagtaggct acctaaaaaa ggtagcccgg aacggatttt atcaccatac ttataaaagg
      541 gatatttttt aagttagtat taagattgtg taatatgatg atatctatat tttatagtat
      601 cagtgctata aaatcaaaaa taagtcacta attaactacc actccaatta taagtctttt
      661 tcccattatt ttttacagtt atatggtatg ctttttaatg gcttcaaata taaaagagtt
      721 taaagcgtaa cacacttatg gggaagtggt ttttggggaa cgctttaaac tcttctttat
      781 tattatctca caatataacc caaatgtctt tccatttaaa aatcaaagta aaacttttca
      841 aatatacaga agtataacta tgtgaaacat cctttcatta atccataaaa ggatacataa
      901 aaaagccact catttgagtg gcaatgaaga aaagctttag cttgtataat actcttcaaa
      961 aaattctaac acagaacgat tcaattggct acattaatgt accctgtagg actcgaacct
     1021 acgaccggac ggttatgagc cgtctgctct gaccaactga gctaagggta gtggttgttg
     1081 ccacataaag ccataaacaa tcaaccagta gaatgcgtgg caacaaacct gttatcgcat
     1141 atcttggagt gtgactattt atgagtgata gtgaagatat gcgtctccat atatgtatgg
     1201 ttgagacaga taaacatgta tgaaaaacgt tgttaaatca acgttttttt attttggtca
     1261 aggagagaaa agtagagaaa tacaaaattt tttgcattga ttttgcattc aaaaaaacat
     1321 tcaaacattt agaaatcccc aatacgtatt caggaaaatc tattttaagg tttacaaaaa
     1381 gaacttttgt tcgtacactc tttttgaggt gaacttttat gctgatggaa ggaaaaacac
     1441 aattatggtt taagcatgtt ccttcaaata agttttttaa ttataaactc tagagaaatt
     1501 aattcatcct aacataaaat aattgttcac tcaaaagttt ttctagaaca attaaaacag
     1561 caggcttcac attcgctata catttgtaag aagtaataat tcctgtacta aaaggcataa
     1621 ttgctttaat tattttttga cttctcttca aacacaagaa gtagttgtgt aataattgca
     1681 gaaataagga ttccaataat agatataaaa tttgacatgg aaacgtctat aacattaaaa
     1741 gaataaagtg ttgaagaaag taaactgatt gaaataaccc ctccgtaaaa aatagtctgt
     1801 gaatataccc actttttttc agaagacgta cttcgtttgg ttctaaaacc tatccctgca
     1861 tttctactct tatcaaaaaa ccaaatattt agaagtaata atattgctat tattaagata
     1921 aaattactgt aaaaatccaa aagctat
//
LOCUS       DACEQH010000145         1874 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.154, whole genome shotgun sequence.
ACCESSION   DACEQH010000145 DACEQH010000000
VERSION     DACEQH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.154"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            388..1770
                     /locus_tag="IW833_002766"
                     /pseudo
     CDS             388..1770
                     /locus_tag="IW833_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          698 a          316 c          339 g          521 t
ORIGIN      
        1 attgtaggtg aacacgaatt ttataaatat ttatggtgtt aatgacaaat gatatccgac
       61 tcctcttcat acgttctgga ctatttgcta atagattaaa atttcagcat ctatttctga
      121 ccataaataa atgagctgcc gaaaagctgc caaatcagac ttgaaaataa aggatcaaaa
      181 acttttaagt ttctgacccc gtaactcaca cttcaaaatc ttacttaaaa aggagtctga
      241 aagaaaaaaa gagtattcaa aatttttttc taactagcta catcacatct atgcattgac
      301 actcaaagaa ccagaataag tcatcagaaa gcaagggggt gcggacataa tcctggacga
      361 aaaaataaac ttaggaggaa tgtccgtgtg ttttcccaca aagagcgtgt aagggcaatt
      421 cagttatttt taaaatacga ttgctcttat gctgcaacca ttcgagaatt agggtatccg
      481 tcgattggtg cactacggaa atggtataaa gagtatttgg aatccggaga gctccatcag
      541 gagcaccgaa aaaaatcaaa gtattctgaa gaacaaaaac gaatagcggt gaatcattat
      601 tttgaatatg gtcaatgtta tgcacgaacc attcgcatgc ttggttatcc taatagagag
      661 tccttgcggc agcggtgtga agaacttgca ccaggagcta gaaaactccg aaaaagtgcg
      721 gtaaagttaa cacaagatca aaaagaagct gtgttgaaaa aattctataa accgcaaact
      781 aataggaaaa atttagccga atctggagga attagtagag ttactctata tcaatggaaa
      841 aacacggttc ttggaaagga ttttccattg tgtatgactt taaaaaatca ggaaaaacaa
      901 aaagagctac ttttaaagga agtagaagac ttacaaaaac aagtacacca attacagctc
      961 gaaaaagcat tactcgaagg agcggctgaa ctgctaaaaa aagaaaaagg cgtcaatctt
     1021 ctttgtctat caaatcaaga aaaaacgata ttgattgatg ccttacgaaa tcagttcacc
     1081 ttgaaagagc ttcttcaaca gctccagttt cctaaaagca gctattttta tcaaaaacaa
     1141 gcacttgaga aaccagataa gtattacaaa gaacgtcaaa ttaattatca cgattttcaa
     1201 tcataacttc tgtgcgtatg gttaccgtag aattcaccaa gctttaaaaa atatgggtaa
     1261 aaagctttct gaaaaagttg tacgtagatt gatgactgag gaaaatctat ttgttaaatt
     1321 ttctagacga aaaaagtatt cttcgtatgc tggggaaatt tctcctgcac accctaattt
     1381 attaaatcgg aattttaaag caaagaagcc aaatgaaaaa tggttaacag atattactga
     1441 atttagaatt ctcgcaggga aaatctactt atcaccactt gtcgattgtt acgatggtgc
     1501 tattgttagt tggactattg gaacaaagcc aacttgcttc actctatgtc taaaaagggt
     1561 tattcaccag ataattcagc gtgcgaaggt ttctttggac gattaaaaaa cgagatgttt
     1621 tatcaacgtg actggaagaa tacaacaatc aatcagttta taaacaaatt ggatgattat
     1681 attcattggt ataataatca acgaattaag ttatctttag gagggctaag ccctcttcaa
     1741 tatcggaaga agcaaagata tatttgttaa aaaggtccaa aaaaaatatc cgcaccccct
     1801 tttttttgta aaaaactatt gcacaataca agtagcctac ggctatcgaa atttccataa
     1861 ctttcgagca agaa
//
LOCUS       DACEQH010000146         1842 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.155, whole genome shotgun sequence.
ACCESSION   DACEQH010000146 DACEQH010000000
VERSION     DACEQH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.155"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            5..733
                     /locus_tag="IW833_002767"
     CDS             5..733
                     /locus_tag="IW833_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1308805.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNRSIILNDP
                     NWCKQANLREISMPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLGYDFFDDD
                     TDPMDTMGHGHILPGLLVLKQTTN"
     gene            763..>1842
                     /locus_tag="IW833_002768"
     CDS             763..>1842
                     /locus_tag="IW833_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HAQ1308806.1"
                     /translation="MVPIRVFGSSGGTSLAILTEAINYASQKGFPIINFSIGSVVYSS
                     TEKAIKNYPGLFVAAAGNNGIDMTDKPFYPASLDIPNIITVGNSSGYERSSDSNYRKT
                     GVDLFARGFRVFSTFLGSKYTYMSGTSMATPHVTGSAALALSLDPDLSTDELKKIILN
                     SVDSFYELSDLCLTGGRLNTNNVVRQVTGKLTASNITLRLNSEWSDEIAKEMCHVHWI
                     DQSGNDLTDQVVVLKNEVDTSNFGDYEVVFASPDLTDQISVTVTVPRLRKLTLGKETA
                     TIDIGSAWDSVIAIDTFQVKAIDDFGNDVTNQVQVTINADIDTINSIQAVIKFEVPEL
                     GLAQIGRLNITAKNSTINGLNEVSIE"
BASE COUNT          636 a          253 c          396 g          557 t
ORIGIN      
        1 gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg tgagtatgtt
       61 tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag aaaggattat
      121 gagccaatta gatgactctc aaatagagta tgtacccaat gagctaattg tcagtttaaa
      181 ggctacacaa agaatttcaa cagagaaaaa taaaccgcaa aatttattgg gtgtaaaagt
      241 tatggtggaa aaagatttga ctcttaggac aaatgaaagc gattataata agcgagatca
      301 agtattatta gtgaaattga acgaagaaat tagtgtggaa gaggcagtaa gaacatttaa
      361 tgcttctgat atggtcaatt atgcagagcc aaactatgtg gttgaactag agccgttgaa
      421 tgatgttgag gagccaaata caactgattc tttaggaaat cgatcaatta ttcttaacga
      481 tcctaattgg tgtaaacaag ctaatttaag agagattagc atgccatccg cttgggaaga
      541 aaccaagggt tctaaggatg tgaaggtagc ggtcattgat tcaggaattg attatcaaca
      601 tgaagatctt tctggaaatg tagatgaaag tttgggatac gatttttttg acgatgatac
      661 agatcctatg gatactatgg gacatggaca catattgccg ggattattgg tgctcaaaca
      721 aacaacgaat taggtgttgc tggaataaat tgggatgtta cgatggtacc tatacgtgta
      781 tttggtagta gtggaggtac cagtttggca atcttaactg aagctattaa ttatgcatcc
      841 caaaaagggt ttccaattat caattttagt ataggtagtg ttgtttatag cagtacagaa
      901 aaagcaatta aaaactatcc tggattattc gtagcagcag ctggaaataa tgggatagat
      961 atgactgata aaccttttta cccagcttct ttggatatac ctaatattat tactgtagga
     1021 aattctagtg gttatgaacg aagttctgat tcaaattata ggaaaacagg tgttgattta
     1081 ttcgctagag ggtttcgtgt ttttagtaca tttctaggaa gtaaatacac gtatatgtca
     1141 ggtacctcga tggcaacacc tcatgtcact ggtagtgctg cattagcgtt gtcactagat
     1201 cctgatctat ctactgatga attaaaaaaa atcattttaa atagtgttga ttcattttat
     1261 gaactatctg atttatgtct tacaggggga agactaaata cgaataatgt cgtacgacaa
     1321 gtaacgggta aacttactgc atctaatatc acattaagat taaatagtga atggtcagat
     1381 gaaattgcta aagagatgtg tcacgttcac tggattgacc agtcaggaaa tgatttgacg
     1441 gatcaagtgg ttgttctaaa aaacgaagtg gatacatcta actttggcga ttacgaagta
     1501 gtttttgcat cacctgattt aacagatcaa atctcagtta cagtgaccgt acctcgatta
     1561 cgtaaactta cgctaggaaa agaaacagca accattgata ttggttccgc atgggatagt
     1621 gtcatagcca ttgatacttt tcaagtgaaa gctatagatg attttgggaa tgatgttaca
     1681 aatcaagtac aggttactat taatgctgat atagatacaa ttaattcaat tcaagcagtg
     1741 attaagtttg aagttccaga attaggctta gcccaaattg gtagactgaa tattactgca
     1801 aaaaatagta caatcaatgg attgaatgaa gttagcatcg ag
//
LOCUS       DACEQH010000147         1823 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.156, whole genome shotgun sequence.
ACCESSION   DACEQH010000147 DACEQH010000000
VERSION     DACEQH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.156"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..737)
                     /locus_tag="IW833_002769"
     CDS             complement(<1..737)
                     /locus_tag="IW833_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAQ1308807.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIG"
     gene            1187..1801
                     /locus_tag="IW833_002770"
     CDS             1187..1801
                     /locus_tag="IW833_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1308808.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIITSVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
BASE COUNT          594 a          345 c          309 g          575 t
ORIGIN      
        1 aagccaatca cacctagtct attcttaatc gtataaggat tccagttagc aagatctcta
       61 atgctcataa tccccatctg attgagtctt tttttcattc gagagccaat tccccaaaag
      121 tccgtcattt caggaatatt ccatactttt tcaggaacgt tttcgtacgt ccattccgca
      181 acaaatcctt cattatgctt tgcttcgttg tctaaggcta gtttagccaa aaggggatta
      241 tctccgacac ctactgtagc aatcaatcct agctcttctt taatattctc ctggatcatt
      301 tgagccagct tctttctccg ttgacttcgt gttccttcag tcgtaaaaag attcagtgat
      361 cgggtcactt ttaagattga ttcatcgatc gagtaaatca gtagatcttc atcagccaca
      421 tatcttctga aaatattatt tacctgcata tttcgcttga tatataggtt catacgtggt
      481 ggaacaacgt gtagtgtttt aggaaatggt tgtggtaagt cacgtggtct actcacattt
      541 gtaataccat accgcttttt tgcttcagga gaagaagcta atatcaatcc tgaaccagta
      601 ttatcagctc gactcataac aacaagttct gttgttaatg gatctaaatt tctttctata
      661 cattcgacac ttgcgtaaaa gcttttcata tcgattaaaa agtaatcatt tactggctct
      721 tttgaataat ccatcataaa taacactcca ttctttcaca ttatacaaac atatgttcga
      781 tattgtaaag aagaaatagc taaatacata aaaaaagaag cataggtatg ctatcaccct
      841 gcttcttctt ttgatttgta tgatcaattt attctatcac tttactaatt aatttgaaaa
      901 tctaataaaa actctcactc taaaagagtg agagactgca aattagagta cggacttttt
      961 tggagttccg ttctttaata ttaacaaatg aataaaatcc ttacaacatt tgagaataag
     1021 ttatcagtag acacttacaa aaaattctca tactatttcc tccatcatta gtttttgtct
     1081 atataaggtt taccctaaat agacactggt ataacattga aaaatatggc ttcctcgtgt
     1141 atgattaggg tgtattttaa atagttgcag aaagtaggta aaagttttga gagttgcata
     1201 catacgtgtc tcatcgatcg atcaaaatga acaacgccag attgaagaaa tgaaaaaatt
     1261 cggtgcagag agaattttca tcgagaaaca atctggcgca acgattactc atcgacctgt
     1321 tttccaagaa gcattagatt acgtccgaga tcaagatatt tttattgtgg aagctattga
     1381 tcgacttgga agaaattatg acgagatcat cacttcagtc aattacttga agaagaaaaa
     1441 tgtgaagctg atcattacta gtttaccgat catggctgaa gcaattggca atcctctcct
     1501 ggataaattc attaaggatc tgatcatcca aattttggcc atgatcgctg aacaagaacg
     1561 gactgaatcg aaacgtcgcc aggcacaagg aattaagatc gcaaaagcaa acggcgtcta
     1621 caaagggcgg cccaaattat acagtgcaga cgcaaaagat ccccaacgtc gccttgttta
     1681 caaaagcatc gttgaagatc ttaaaaatgg tgttgcgatt gctaagattg ctaaagacta
     1741 caatgttacg agacaaaccg tgtatagaat aaaaaaagac agcatggtca atgacaaata
     1801 gttgatcatg ctgcttttat tat
//
LOCUS       DACEQH010000148         1823 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.157, whole genome shotgun sequence.
ACCESSION   DACEQH010000148 DACEQH010000000
VERSION     DACEQH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.157"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(277..732)
                     /gene="tnpA"
                     /locus_tag="IW833_002771"
     CDS             complement(277..732)
                     /gene="tnpA"
                     /locus_tag="IW833_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1308809.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            975..1823
                     /locus_tag="IW833_002772"
                     /pseudo
     CDS             975..1823
                     /locus_tag="IW833_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          576 a          356 c          341 g          550 t
ORIGIN      
        1 attatttctg cgccagcatt ttctaatgtt tgaatttgtg ttttgtaatc ttggttgctt
       61 gtgctaattc ttgaatagcc gtaaattgcc aaaatttcat caccctttct ggacataagt
      121 ttctgacacc agttaaaatc aagtctatca gtaacccata cacccgtgag gacgggtgta
      181 tttttaatcc ccctagaagg ggtcgacact cgctgcccct taaaagggga tttttaaaag
      241 accgccaact gctcatcttc actcacttac ttctctttat tttttaaatg gttctacgta
      301 ctctctttta cttataatat atcgaatgcg atcatcagct tcttgttcac ggatgtattt
      361 ttcatttgtc ttttgattca atccgactgt actcacatag tatctcttcg accaaaagga
      421 tttgtttccg tatttatatt tgagatttgc gtgctgctcg tggattagta ccgcactacg
      481 acctttcaga taccccataa aactggatat acatattttt ggtggtactc gaacaagcat
      541 gtgaatatga tctagcattg catgtgcttc tatgatttcc acatctttca tttcgcacaa
      601 cttcctaagt atttttccaa gatcttttcg taattgccca tagattactt ttcgttgata
      661 ttttggtgta aaaataatat gatacttaca attccaacga gtatatgcta aacttttatc
      721 gtcatttgac atgaaaaaac tcctttcgat ctgatgagcg gttggtagcc actcactagt
      781 atatcgaaag gagtttttat cgctaaagat acttcttcca cgcgtagaaa cgcgcggttt
      841 tcttgtttcg cacgctttac gcacaaaact gactgaagtc attaagaaaa taaaagggtc
      901 taaaactttt aagttttgta ccgaccccca aaagttagac caaaaaatct aacttttgga
      961 ggtcggtatt ttttatggct aagtatactt ttgaacttaa attaaagatc gttcatgatt
     1021 acttggatgg taaaggcgga agtgattatc tggcaaagaa atattccatt aaggctccat
     1081 cacaagtgaa gcgctggatc aatgcctatc aagaatttgg tgaagagggt ctagtaagaa
     1141 agcgacaaaa gaaaaaatat tctgttcaat ttaagctgga tgcgatagag ttatatctaa
     1201 caagtgagct gtcctatcgt gaagttgcca acacacttaa catgaacaac cctaatttga
     1261 tcgctagttg gatgcgtcaa ttccgtgagg gtgggatcga tggactctca aaaacgaaag
     1321 gatgtccacc tatcttgtct aagaaaaatg aacccaaaaa taagaaaaaa tctacaacta
     1381 aagcaacgtc taaagaacgt gaacgcatcg aggagttgga gaaacgagtc cgttcccttc
     1441 aaattgaaaa tgccttttta aaagaattga ggaagctgcg aaaacaggaa gcccaacaac
     1501 gacgaatgaa gcaatcgcac gaatcatcgc aagcctccga ggaccgttta aattagttga
     1561 gcttctagaa actttaaagt tccctaaggc aatctttatg tactggcaaa aacgattgga
     1621 ccgtaaaaat ccagatcaag agattgaagc ggaaatgctt aaagttcggg agaagcacaa
     1681 ggattatggc tgtttacgga tgaccaatga actaagaaac cgcggtttct ctatcaataa
     1741 aaagaaagtt cagcgcctga tcaagaagct aggaataaaa gtaacgacat acacacgtaa
     1801 atcacggcgc tacaattctt atc
//
LOCUS       DACEQH010000149         1701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.158, whole genome shotgun sequence.
ACCESSION   DACEQH010000149 DACEQH010000000
VERSION     DACEQH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.158"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..255
                     /locus_tag="IW833_002773"
     CDS             <1..255
                     /locus_tag="IW833_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308810.1"
                     /translation="NFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSVI
                     SEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD"
     gene            258..411
                     /locus_tag="IW833_002774"
                     /pseudo
     CDS             258..411
                     /locus_tag="IW833_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            434..1639
                     /locus_tag="IW833_002775"
     CDS             434..1639
                     /locus_tag="IW833_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1308811.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT          547 a          322 c          377 g          455 t
ORIGIN      
        1 aatttcagag tggctttagg tcgtatgtct gaaatgggct atggcatgat gtttggcagt
       61 gacgtacaaa aggatttctt cttaaagcga atcaaaggtc gtggctatgt tgatgtagga
      121 acaagtgtca tatcagagtt ttatactccc cttgtaccaa aaggatatga tttcttggag
      181 gaaattaaaa agttatccaa cagcagacag tccacgcagg cgacgtgcga agcggaagtc
      241 gcaggtgtgg actgatcttg ctggctggtg tggcaatagc cacgccagca cttaacccct
      301 cggtatctaa caggggggta caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc
      361 tttggttaca agggatttac aaaatttcag cgtatgtcaa atgggcttta aaagttgaca
      421 tacgcctttt tgattggagg gatttttact gaatgaacaa acttggttac agcatttaaa
      481 agaaaaacgc ttggcttatg gactatctca aaaccgttta gctgttgcga ctggtattac
      541 aaggcagtat ctaagcgata ttgaaacagg aaaagtcaag ccatcagagg atttacagca
      601 gtccctttgg gaagctctgg aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga
      661 ttatgtaaga attcgctttc cgacaacaga cgtacagcag gtggtcgaaa acatcttaca
      721 actgaaactg tcctattttc ttcatgagga ctatggtttc tattcttatt cagagcatta
      781 tgctttaggc gacatattcg tcctttgctc ccatgaactg gacaaaggag ttctggtgga
      841 attgaaaggt cgtgggtgca gacaatttga aagctatctt ctggcacaac aaagaagctg
      901 gtatgagttc tttatggacg ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc
      961 cattaacgat aagacaggga ttttaaatat ccctgtactc actgaaaagt gccaacagga
     1021 agaatgtatc tccgtcttcc gcagttttaa aagctatcgc agtggcgaac tggtacgcaa
     1081 agaggaaaag gaatgtatgg gaaacaccct ctatatcggt tcattacaaa gtgaagttta
     1141 tttctgtatc tatgaaaagg actacgagca gtacaagaaa aatgatattc ccattgaaga
     1201 cgcagaagta aaaaaccgtt ttgagattcg attgaaaaat gagcgtgcct attatgcagt
     1261 ccgtgattta ctcgtctatg acaatccaga gcataccgcc tttaaaatta tcaatcggta
     1321 tatccgtttt gtagataaag acgattccaa acctcgttct gattggaaac tgaatgaaga
     1381 atgggcttgg tttattggga acaatcgtga acgattaaaa ctaaccacaa aaccagagcc
     1441 ttactccttc caaaggacgc tgaactggct atctcatcaa gttgccccga ccttaaaggt
     1501 tgcgattaaa cttgatgaaa tcaaccagac gcaggttgta aaagacattc tcgaccatgc
     1561 gaaactgaca gaccgacaca agcagatttt gaagcaacag tcagtaaaag aacaggacgt
     1621 gataacaaca aaaaaataac tcaaatacaa attcattgaa tatagagagg agaacatttt
     1681 tatgaatttt ggacaaaacc t
//
LOCUS       DACEQH010000150         1700 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.159, whole genome shotgun sequence.
ACCESSION   DACEQH010000150 DACEQH010000000
VERSION     DACEQH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.159"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            466..1065
                     /locus_tag="IW833_002776"
     CDS             466..1065
                     /locus_tag="IW833_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1308812.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1129..>1700
                     /locus_tag="IW833_002777"
     CDS             1129..>1700
                     /locus_tag="IW833_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308813.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT          612 a          257 c          321 g          510 t
ORIGIN      
        1 acacaaaatt attgacgctc ccaactaaaa gtgtcaaaat aatgatgaaa tcatttagtt
       61 cttataaatt agaaaagaga aaataaatat tccatatcta ctttattgga tccgtaacat
      121 gactaaattc tgaatacttt ttaaagctta tcatttggta aaactgaata taagttaatt
      181 atatagactg aaaattttga attttcagaa tagaataact taaaagtact gacaaaattt
      241 tttaaagggg caatattaat gttctgagat aatcaaatcc taccgaaaat atcaaaaatc
      301 caatgtgaca caatcagatt gtgtctcatt cgagggaatc acacaatccc acgtggctct
      361 aggcagaatt gaatttttgc ttggggcttt ttccttatat tgattttgaa tgagacttat
      421 tatatgttat aatgcaatta agtatcattg gagagggcga agaaaatgaa gacggtagaa
      481 cctattcgag acaaaaaaaa gatcgatgcg atgaaggcaa tcttggcttc gggaaaatat
      541 ggccaacgaa atttggttct tttttcgatt gggatcaaca ccgcctatcg catttctgat
      601 ttgcgtcaat tgaagctggc cgacgtgctg gaaatttcac gcggtcgcgt ggtggtcaaa
      661 gatcgattag cgatgaagga acaaaaaaca gctaaacata attcggtcta tatttccaac
      721 aaattgcgaa aaacgatttt ggagtacgtt caacaggaat ttcctgatca actccttcag
      781 caagattttg acggttattt gttccctagt cgaaaaggga cgaatgaacc gctcaaccgc
      841 caaagtctgt ggcgaatcat tcatgatgct ggcacggcaa tcggattgaa agacattggc
      901 ccacattcca tgcgaaaaac gttcggttac tttctctata aacaggggac caaaacagaa
      961 atcattcagt ccttgttgaa ccattcttct cagcgcgaaa cattgcggta tattggtatt
     1021 acacaagaag acaaagatac agctgtaaaa agcttagatt tataatataa aagttaagtt
     1081 taaacatgga tagttttaaa ttagtagatt gagtgttggg aggaaagaat gagtacatta
     1141 tatattatag gaaacggttt tgatttaagt catggtttaa aaactagata cactgatttt
     1201 tcaaaatggg ttgaaaaaag tcatagtgaa atattcgata aaatagacac aatgataaat
     1261 gaaaataatg aggtgagaag tacagctgat gataagatag gttggtttaa ttttgaaagt
     1321 agtttgcatg agttaattca aagtccagtc tttgaagatt atgtagaaga gtctaaggat
     1381 tatctcatag gttatggaca tgaagattgg tcagattctg cccatcatga ttttcaaaat
     1441 atggtttatg attatgttgt ttctctagaa gaattacaac aacttttcaa ggaatggata
     1501 cgcaaagaaa tacagccgac aatagaacat aaaattccaa aataccaatt ttcgtccgat
     1561 gtactattct ttacattcaa ttatacagat gttttggaaa ttctatacaa gcaaccgaaa
     1621 gaaaacatat atcatattca tggcagttat gacgatataa taatcggcca ctccgagaat
     1681 tttgacaact ttataggaga
//
LOCUS       DACEQH010000151         1618 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.160, whole genome shotgun sequence.
ACCESSION   DACEQH010000151 DACEQH010000000
VERSION     DACEQH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.160"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            102..1568
                     /locus_tag="IW833_002778"
     CDS             102..1568
                     /locus_tag="IW833_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ1308814.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          452 a          292 c          335 g          539 t
ORIGIN      
        1 atagaaaatt agtttatatt tcttaaataa gttttttagt taaatttttc ggtaaagatg
       61 tataatattt tctgaaaatt taaagaattg gggtatagat tatggaaaaa cagcaagaca
      121 agggcttctt cgggcagccg aagggattgt caactttgtt cttcactgag atgtgggaac
      181 gatttagtta ttacggtatg cgtgcattac ttatctatta tatgtacgat acagttgcca
      241 acgggggact tggattacca cgagcaacag cattagctat catgtcgatc tacggatctt
      301 tgatctacat gtctagtatc gtcggcggat gggtatctga ccgagtgtta ggagcaaaac
      361 gcactgtatt cttcggtgga gttttcatta tgcttggcca catcgtcttg gctttaccat
      421 ttggtgtaag tacattgttt gtttcgatgg cattgattat tatcggaacc gcctttttga
      481 aaccaaatgt ttctggcatg gtcggacatc tttattcaaa gactgacttg cgaagagatg
      541 caggattttc gattttttac atgggtgtca acttaggggc attattagca ccactaatcg
      601 ttggtacaat cggtcaagaa tataactatc atttagggtt ctctttagct gctattggga
      661 tgttctttgg attattacaa tacatcatcc aaggacgcaa gacattagac ggagtgggta
      721 caacaccgcc gaatcctttg acaactgaag aacgtaaagg attcatccgt aacttgatca
      781 tcgcacttgt tgttatcgct gcagtattcg gcggggcaca actaactggt catctgacaa
      841 ttgacttttt tgtcaataca atcagtgtat tggggattat cttaccgttg tattacttta
      901 tcaaaatgct gacttcaaaa gatgtaactg ctgaagaaaa accaagagta tgggcttata
      961 tcccattatt ccttgcagca atcattttct ggtcattaca agaacaaggt tcatctatct
     1021 tagctttatt tgctagtgaa cgtacacaat ctacgctatt tggtgtaacg atccaagcaa
     1081 gctggtatca atcattgaat ccaattttca tctttattct gacaccagta ttcgtaacga
     1141 tgtggacgaa acttggtgaa cgtcaaccat ctacagtagt gaaattctct cttggacttt
     1201 tatttgcagg attatcgtac ttgctattga tgttgccggg tatgctttat ggtacaagtg
     1261 gaaaagtaag tccattgtgg ttgttgggaa gtttcttcat cgtggttatt gctgaattgt
     1321 gtctttcacc agttggtttg tctgtgacaa caaaattagc accacgagca tttgaatcac
     1381 aaacaatcgc tatttggttc ttagctgatg cttcttcaca agcaatcaat gcacaaattg
     1441 cacgcttcta cacaccggga acagaatctg cttacttcgg tattatcggt gcagtagcaa
     1501 tcgtcggtgg tatcttgcta tttactgtga aagaaccaat caaaaaacta atgggctcta
     1561 ttcattaatc aatgaacata aaaacaggaa ttgcttattt gcagcagttc ctgttttt
//
LOCUS       DACEQH010000152         1594 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.161, whole genome shotgun sequence.
ACCESSION   DACEQH010000152 DACEQH010000000
VERSION     DACEQH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.161"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1485
                     /locus_tag="IW833_002779"
     CDS             <1..1485
                     /locus_tag="IW833_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1308815.1"
                     /translation="CEAKFSENSYGFRPNRSVENAIKATYNRLQISQLHYVIEFDIKG
                     FFDNVNHSKLIKQIWAMGIRDKHLIFILKRILKAPIKMTNGTITYPEKGTPQGGIISP
                     LLANIVLNELDHWVESQWQENPVTKNYVVHINKSGSPCKSNAYKEMKKTKLKEMYMVR
                     YADDFRVFCRYKESAEKAKIAITQWIEQRLKLEVSQEKTRIVNVRKRYSDFLGFKIKM
                     IPRRKKLVVKSHISDKQFKNQKDKLVSQAKKIASPSKGKTELEETRLYNSMVLGMQNY
                     YQIATNVNLDCMKLNRAVMAIFTNRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYV
                     AGEGEPIFPVGYIQHRKPMAKVYLANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENR
                     TIEYADNRISLFSAQHGKCAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAV
                     HRLIHAKTEETIQKYIALLKLNKVQLIRLNKYRVLAGNQELNLI"
BASE COUNT          632 a          251 c          307 g          404 t
ORIGIN      
        1 tgcgaagcta aatttagtga aaacagctat ggttttagac ctaataggtc agttgaaaat
       61 gcaataaaag caacctataa ccgcctacaa atcagccagc tacattatgt gattgaattt
      121 gatattaagg gtttctttga caacgtgaac cattccaagc tgataaaaca gatatgggct
      181 atgggaatac gggacaagca cttgatattc attttaaaaa ggatattaaa agctccaatc
      241 aaaatgacta acggtacaat aacatatcca gagaaaggaa ctccgcaagg gggaataata
      301 tccccactac tagcaaacat tgttcttaat gagttagacc attgggtaga aagccaatgg
      361 caggaaaatc ctgttacaaa aaactatgtg gttcatatca ataaaagcgg tagcccatgt
      421 aaaagcaacg cttacaaaga aatgaagaaa acaaagttaa aggaaatgta tatggttcgt
      481 tatgcagatg attttagagt tttctgccgc tacaaggaaa gtgctgaaaa agccaaaata
      541 gcaatcactc aatggattga acaacgatta aaattggaag tatcacaaga aaaaacaaga
      601 attgtaaatg ttagaaaaag atactccgac tttcttggtt tcaagattaa aatgataccg
      661 agaagaaaga agttagttgt taaatcacat ataagtgata aacaattcaa aaaccaaaaa
      721 gacaagctgg tatcacaggc aaagaaaatt gcttctccct caaaagggaa aacggaatta
      781 gaagaaacaa gactttataa ttcaatggta ttaggtatgc aaaattatta tcagattgca
      841 acgaatgtaa atctggattg tatgaaactg aatagagctg taatggctat cttcactaac
      901 agattaggta caaacaaaag gggcagattg agaaaaacag gcagacaact tacgacaaag
      961 gaggcaacaa gatatggtaa atctaaaatg cttcggtatg ttgctggcga gggcgaacct
     1021 attttccctg ttggatatat ccaacacaga aaaccaatgg caaaggtgta tttagcgaat
     1081 tgctacactc tagagggaag aaaatttatc cacaccaatc taagtgttga taaattactg
     1141 atgtaccagt taatgaaatt atctttggaa aatcgcacaa tagaatacgc agacaatcga
     1201 atttcgctat tttctgctca acatggaaaa tgtgctataa ctctggaaga atttcaacaa
     1261 gctaatgaaa tacattgtca tcatattgta ccaactggag ctggtggaaa tgacgattat
     1321 aaaaatctta ttttggtaaa agaggcggta caccgtctaa tacacgctaa aacagaagaa
     1381 acaatacaaa aatatatagc tctattgaag ctaaataaag tccagctcat aagactaaat
     1441 aaatatagag tattagctgg caatcaagaa ttaaacttga tataggtcta ctacataaat
     1501 acttttcaat ataaacacga tggaacgcgg agtgcggtga aagtcgcacg ctccgtgtga
     1561 atggggggaa aagtttgaga tatttataat atca
//
LOCUS       DACEQH010000153         1490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.162, whole genome shotgun sequence.
ACCESSION   DACEQH010000153 DACEQH010000000
VERSION     DACEQH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.162"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..132
                     /locus_tag="IW833_002780"
     CDS             <1..132
                     /locus_tag="IW833_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1308816.1"
                     /translation="STSPSTATFEQRNTATPNNNGLNASQNPFGGQSIDISDDDLPF"
     gene            177..410
                     /locus_tag="IW833_002781"
     CDS             177..410
                     /locus_tag="IW833_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308817.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(569..691)
                     /locus_tag="IW833_002782"
     CDS             complement(569..691)
                     /locus_tag="IW833_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ1308818.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            881..1105
                     /locus_tag="IW833_002783"
     CDS             881..1105
                     /locus_tag="IW833_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308819.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
BASE COUNT          482 a          292 c          286 g          430 t
ORIGIN      
        1 tccaccagtc caagcaccgc cacctttgaa caacggaata cagcgacgcc taacaacaat
       61 ggattgaacg cttcacagaa tccatttggt ggacaatcaa tcgatattag cgatgatgat
      121 ctaccgtttt agtttgagaa agaaggagta agcatggcaa aaaaattagt ctgacgatgg
      181 atctattgat acccgtaatt gcatttatag acgggatttc tttgggcgtc actgaaaatc
      241 ctttggtaaa tagaattgcg aaaggatcac tatttcttgt attcgtgtat cttgtctgtt
      301 cttatctttt aaaaagagca ggaaaggata attccttttg gcgattgact aaagtcacag
      361 tagcagcgac agctgttata cttacggcta ttctaccgct ttatatgtag agttaacggg
      421 aaatcgttga acttcttgcg aaatattgct ataatgaaca tgaaaaagag gagttgcgct
      481 aacaacccct cattaacagc accgattaaa gaacggtggc agaatattag ttatttggaa
      541 ataaccgctg acctgctaaa gtagacggtt atttcttttt gtcattttta agcatattta
      601 caatcaaact aatcaaagca atggtaaaca taccaaaacc aagaatggtt tgaattgtct
      661 catatgcgga caaaaggctt actcctttca tagattttag cacttacatt cataagcacc
      721 accacctttc cagaagtcgc caccgtcttc aaactttact gtcttgacta ttatagcata
      781 tctaaaatcg aataaaaatt ctcaatttta gctgtttttg ctataatttt agcgaggtga
      841 tcgtgatgga acaatatatt ggtttactcg ataaattagg gtgatcaaga cacagccatt
      901 gcttgtgcga tttacattac gcacgagtca tgaaccaatc aattgtgtag tggccaatat
      961 agaaatagcg gataaattgc tgattttgga agaaggcaag tatactattt cagtgacagg
     1021 tcacttcaat aaacgaaagc aactagtcat tgaatcaatg caaattagaa accctgatca
     1081 ttttacgcga tcgatgagca tataaaagaa gagctgcagt cctaaatgag gattgtagct
     1141 ctttttgttt tacatagaaa cagtaaaaat gaaaaggcct gtttaggaag gcacaagcgc
     1201 cttctcaatt gaaagacctt tctctcactc caaccgccac cgccgttcga gaagaaagga
     1261 agtgaacgct acgcgttcac taagataaag ggtcttgcat agcaagacaa aatagaaaaa
     1321 atttcctctc accaaaaggt tcgctcctgt tcgttatctc tttattgatt tgtcagaccg
     1381 gcaacaagtt gccttttatc accaaaaagc gacaaggaca agggtaaatg gcacgctgcg
     1441 cgcccttgtc ttgtcgcttt tcgttgattc attctggcca acaaatcaat
//
LOCUS       DACEQH010000154         1477 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.163, whole genome shotgun sequence.
ACCESSION   DACEQH010000154 DACEQH010000000
VERSION     DACEQH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.163"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(981..>1477)
                     /locus_tag="IW833_002784"
     CDS             complement(981..>1477)
                     /locus_tag="IW833_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ1308820.1"
                     /translation="ELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTPAENLAYQV
                     GWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKELEEILNRNI
                     DDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTFRTKIRKWK
                     RLAL"
BASE COUNT          463 a          282 c          209 g          523 t
ORIGIN      
        1 tactataccc tatactttta cttggtatat ttatgaataa tcgcaaattt acccctatac
       61 tactagtttg taagttagat taataaaaat actaaagtta tttgttcttt tattaaatac
      121 acctacaact tataaattga tggtatttag ataacacgaa agaataaaaa taacagctga
      181 tagtaaagtt tgttgacgat tgctactcaa tcgaaagtga gttagtgcgt atgaacttgc
      241 ttctgaaaac ttatgaaatg aaagaccttt tacatcttgc agaaatcggt actgggatta
      301 aactaagcaa taaaaactaa tgttcccctc attctttgaa cttctactat ttgatttatt
      361 tattctgata aatgacgatt tcaccttcag gatcttcaaa tcccgtatag aatttattta
      421 agatctcttc agtatcagta ataggatagg cgataggcca catacccttt gatggttaga
      481 ataaactgct caaaatctgt atcgtatgtt ggctttagca tattaacaaa ggctatctcc
      541 aaattataaa ccataagcgt tgaatcgttc ttaacaattt caattcttcc taacccaaag
      601 tcagaagata taattaccgg acttgcctta cccttcatgt gggttgttga gattccatat
      661 ttaaagggaa ttgtcggtac tatcggcggt ctgaaaaaca tcccaaaaat aaaaaaatct
      721 ttcccctgct agtctcctaa gtccctttgt agcggatatg gcaccataat ctcattttat
      781 cgaagtgagt catggtttat ctgttggcca accaaaattg atacaatcta acgatgcacc
      841 ctctacggca ttctagggga gtgcacttgc attcacccaa taaaaaccgg cagttagcaa
      901 agaacttatg tagtcgtatg ttcttactgt taacatacaa gaggtctcct ttccacaatt
      961 gcttagcagt aaacgggaat ttacaaagct aatctcttcc attttcttat ctttgttcta
     1021 aaagtcccaa atggagcaac tgtatttaca tgaataaatt tatatacttc ccatacagcc
     1081 gttttggttg cctcgtctgc ccattttctt ttgtgtgcag tgaatagatc ttcatcactc
     1141 attgaatcaa tcatcatgtt aatatcatca atattacgat tgagaatttc ctctaattct
     1201 ttcaatggta gatgtgcata ggtatttgta aaccacttat ataactctcc taactgattc
     1261 catttaaatt gatcagaagg cgttttaaca gataatccat ttctttcatc attttcccat
     1321 tttagaatta aattcgtcca accaacttga tatgctaaat tttcagcagg tgttctatcc
     1381 acttcctcaa ctcttttatc cttcaattct tcaggaatag tttcaaattc taaaatatat
     1441 ttagtataag tcctatttat ttcttttttt aattctt
//
LOCUS       DACEQH010000155         1456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.164, whole genome shotgun sequence.
ACCESSION   DACEQH010000155 DACEQH010000000
VERSION     DACEQH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.164"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            108..1040
                     /locus_tag="IW833_002785"
     CDS             108..1040
                     /locus_tag="IW833_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1308821.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="IW833_002786"
     CDS             1112..>1456
                     /locus_tag="IW833_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308822.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DACEQH010000156         1445 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.165, whole genome shotgun sequence.
ACCESSION   DACEQH010000156 DACEQH010000000
VERSION     DACEQH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.165"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            97..711
                     /locus_tag="IW833_002787"
     CDS             97..711
                     /locus_tag="IW833_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1308823.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(735..1085)
                     /locus_tag="IW833_002788"
     CDS             complement(735..1085)
                     /locus_tag="IW833_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1308824.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1079..1342)
                     /locus_tag="IW833_002789"
     CDS             complement(1079..1342)
                     /locus_tag="IW833_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAQ1308825.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          463 a          271 c          229 g          482 t
ORIGIN      
        1 taccctaact agacacgacc gcgacattga aaaatctggt tttctcgtgt atgattaggg
       61 tgttttttaa atagttgcag aaagtaggta aaaattttga gagttgcata catacgtgtc
      121 tcatccatcg atcaaaatga acaacggcag attgaagaaa tgaaaaaatt cggtgcagag
      181 agaattttca ttgagaaaca atctggcgca acgattactc atcgacctgt ttttcaagaa
      241 gcactggatt tcgtccgaga tcaagatatt tttattgtgg aagctattga tcgacttgga
      301 agaaattacg acgagatcat cgcttcagtc aattacttga agaagaaaaa tgtgcagctg
      361 atcatcacaa gtctaccgat catggccgaa gcaattggca atcctctcct ggataaattc
      421 attaaagatt tgatcattca aattttggcc atgatcgctg aacaagaacg taccgaatct
      481 aaacgacgtc aggcacaagg cattaagatt gcaaaagcga acggtgttta taaaggacgt
      541 cctaagctat acagtgccaa tgcaaaagat ccccaacgtc gtcttgttta taaaaacatc
      601 gttgaggatc taaaaaaagg tgttgcgatc gctaagattg caaaagacta caatgttacg
      661 agacaaaccg tatatagaat aaaaaaagac agcatggtca atcacgaata gttgatcatg
      721 ctgctgtttc tattttattt gcttactttt tgaaaaactg ttctaacttt catcaataac
      781 tcctcaacta aactatcatg cgctttttcc aaaaaaatac actctctggc ttcataatca
      841 attgtcgtta gttgatctaa taatactttt cctgttgtct tcatttcata ttctgagttt
      901 atatttacat atagcggata gtttctagtt gtactagaaa ttggagcaac tgtaacaacg
      961 ttactataat ccgcaataat atcatgactt aaaacaatat aaggtctttt acctgtctgt
     1021 tcgtgtccag atcgaggtgc tgtattcaat aatagaatat cgccttgtct aggttttctc
     1081 accatttttc attacctact gcttcaccta aattttgaat cgtcacatga tttctttcat
     1141 cagtgtaatt ttcaaataac tgttcaattg ttaattcatt ttctatatta gccgcttcga
     1201 taattagccg cttctgaccg tttacttctt cttgtattat cttcactttg tcttctgtat
     1261 gaattccaag atattccata atatactttg gcaatctaag gccattacta tttccccatt
     1321 tttttatagg taacatttcc atagtagaca cctctttctg tatatacata gtataacata
     1381 cataagaaaa aacgtatata cttaattatc cttttctatt aaattagatt aacattccat
     1441 gaatc
//
LOCUS       DACEQH010000157         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.166, whole genome shotgun sequence.
ACCESSION   DACEQH010000157 DACEQH010000000
VERSION     DACEQH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.166"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(150..1337)
                     /locus_tag="IW833_002790"
     CDS             complement(150..1337)
                     /locus_tag="IW833_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ1308826.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACEQH010000158         1407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.167, whole genome shotgun sequence.
ACCESSION   DACEQH010000158 DACEQH010000000
VERSION     DACEQH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.167"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..265
                     /locus_tag="IW833_002791"
     CDS             <1..265
                     /locus_tag="IW833_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1308827.1"
                     /translation="KNAYKAGIDFVGTTLSGYTEESKKQDGPDIDLIKKLVEEGICVI
                     AEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQEIASRFVKALS"
     gene            279..>1407
                     /locus_tag="IW833_002792"
     CDS             279..>1407
                     /locus_tag="IW833_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1308828.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPF"
BASE COUNT          421 a          197 c          297 g          492 t
ORIGIN      
        1 caaaaatgca tataaagcag gaatagattt tgtaggaact actttaagtg gatatactga
       61 ggaaagtaaa aaacaagatg ggccagatat agatttgatt aaaaaattgg tagaagaagg
      121 gatttgtgta attgctgagg gaaggattca tactccagaa caagcaaaag agataaaaaa
      181 aataggagtt gcaggtattg ttgtgggtgg tgctattact cgaccacagg aaattgcatc
      241 aagatttgtt aaagcattaa gttaggggga gaaataaaat gtttaaaaaa ttttcacaat
      301 taggacgcgc tttcatgtta ccaattgcta tattaccagt agccggatta ctattgggac
      361 tgggaggagc gttaacaaat gagagtgcta taaatgctta tccattttta gatcaaccgt
      421 ggttgcatac aattttaagt attatgagtt atgctggaaa tgcagttttt gcaaacctag
      481 cactaatttt tgcaattggt attgctgtag gacttgcaaa tggagataaa ggaacagctg
      541 gtttagctgg tggagtagct tatctagtat atactgcaac tattagtggg tttcttgaac
      601 ttttttcagc aaaagatgca acactagata caggtgtagt gggatcaatt gttgttggtg
      661 gtactgttgc atttttacac aatcgttata gaaaaattga attaccacaa ttcttaggtt
      721 tttttggagg atcaagattt attccgattg tttctagttt agctgcaatc attattggtt
      781 ctttctttta tttaatttgg ccaccaattc aaaatggttt agtagttgct ggtgaacaaa
      841 ttgcccaaat gggtagttta ggaacttttt tatatggttt tttattgcgt ttaacaggtg
      901 ctgttggact acaccacacc atttaccctt tgttctggta tacatcttta ggtggtaccg
      961 aaactgtagc tggatcaaca attgctggag cccaaaatat tttctttgcc caactagctg
     1021 atccaaacca cactggtctg tttacttatg gtactagatt ttttgcagga agatttgcaa
     1081 caatgatgtt tggattacca gcagcatgtt atgcaatgta tcgtgcaatt cctaagaaaa
     1141 atcgtaaaaa gaatggtggg ctttatttta gcggggccct tacatcattt ttaactggaa
     1201 ttactgaacc agttgaatat atgtttttat ttgttgctcc ttggctttat gtgattcatg
     1261 cattcttaga tggattatct ttttattttg ctgatatctt aaatattcgt gtagggaatt
     1321 ccttttcagg tggtttgatc gactttttat tattcggtgt acttcaaggg aatgacaaaa
     1381 ctaattggtt aaaattaatt ccttttg
//
LOCUS       DACEQH010000159         1405 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.168, whole genome shotgun sequence.
ACCESSION   DACEQH010000159 DACEQH010000000
VERSION     DACEQH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.168"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            244..444
                     /locus_tag="IW833_002793"
                     /pseudo
     CDS             244..444
                     /locus_tag="IW833_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            931..1110
                     /locus_tag="IW833_002794"
     CDS             931..1110
                     /locus_tag="IW833_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308829.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1156..>1405)
                     /locus_tag="IW833_002795"
     CDS             complement(1156..>1405)
                     /locus_tag="IW833_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1308830.1"
                     /translation="IAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQKRL
                     VFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
BASE COUNT          463 a          253 c          264 g          425 t
ORIGIN      
        1 attaatgtac ttaaaagaga aaaaaacaag taattaatat gagaacacca cgcatagttt
       61 ttattttgag atattctaga atgaaacaca ttaagaacga aagcaaagca aaagacgcca
      121 gaacttctac taccttctaa gttttttcat atttgtttgt tatgctatac atagatctgc
      181 ataattagac ttgcgctgcc cgttattgaa gtttcaggca gcgctctttt tgggggatcg
      241 ataatggaag aagaaaaaat atttgagaaa cgctggcagc tagcatcaag tgaacaaaga
      301 gcaagataca ataatttgat cacctcctac cccactgttg actagacata taaggaaaag
      361 aaatacttac tttggctgtg ccagttagat atagatacct ttgaaacatt tgaagtgatc
      421 ctagataaaa ttaaacagaa ttgatcagaa acaagcgaag ccttctctcg gttcgcttgt
      481 tttgctattt attagttggc tcagggcaac tctaagctcg tttgaaagaa aaccgatgga
      541 taatcccctt gtttgtacca ctttagaata catggatgta cgtattgcca ttgactccta
      601 ttagttactg aaaaaaaatt taaacaagtt gagtattgca gataaatagc tccagtgagg
      661 tgaataactg ctccactcaa ggcaatagat agctccatcc agggcaacaa actgctccac
      721 tttcatattt aatagatgaa taaccttctg tataatagaa aggcatacct ttcggtatga
      781 caacttatac agaatgttat tttattggtt cactatcgaa aaattttgga gctttatttt
      841 tccggtattt ctcaacgaac gattagttta actgtgggaa gctccagaca tacgattcga
      901 gaagttactt ctcgagcaga caggaagggg gtgactgaac taaccgctga tatgagcgat
      961 acttggctgc aggaatatct ttttccggaa aaacaaacaa cagcacgagg ttaccttcct
     1021 gtagactggg aatatgttca taaagaacta atgaaaaaga atatgacatt gaaactcctt
     1081 catagagaat atggggtaca tggaacatag aaacgaaaat gcacggtaga aaagcgaatg
     1141 aatatagtat aaattctaca aatcattcgc tttctttatt ttatatatgg ttggtcgaga
     1201 gagaccattt tctttggcaa tttctgcaac tggaactcct tgatttaaca tgtccaccac
     1261 agttttaaag actaatcgtt tttgcggatc tgttgagttc tcagaatatt caattggtcg
     1321 ccccttatat cgaccagctt ttttggccaa taatactcct tgccgctgac gttcacggat
     1381 tttctcacgt tcgttttgtg caata
//
LOCUS       DACEQH010000160         1387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.169, whole genome shotgun sequence.
ACCESSION   DACEQH010000160 DACEQH010000000
VERSION     DACEQH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.169"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..815
                     /locus_tag="IW833_002796"
     CDS             <1..815
                     /locus_tag="IW833_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1308831.1"
                     /translation="LNGLVKKGTDGKLTFTGVSLPEGKKPLILSQDDVSYYEYMDNSG
                     FPSKLIVDKQNQIKNIYIDNKKETVGDYDMVPLIDSFIKKHPDFSYQGAKGTLALTGY
                     NGVLGYRTSKSEYGDNEKTHKEIEAAKKVADQLKEDGWSFASHTWGHLNMTQASLADI
                     QQDNERWQNEVAPILGKTNILIYPFGADISDWQPYSEANQKFAYLKQQGFDIFCNVDA
                     STPAWGQLGTDYYRNARINIDGIRFEADLKGENPILDHFINVKEMYDQKDRG"
BASE COUNT          474 a          240 c          268 g          405 t
ORIGIN      
        1 atctaaatgg attagtcaaa aaaggaacag atggaaaact gacctttaca ggtgtttctc
       61 ttccagaagg aaagaaaccg ttaattcttt cgcaagacga tgtcagctat tatgaataca
      121 tggacaattc aggatttcca agtaagttga ttgtcgataa acagaaccaa atcaagaata
      181 tttatatcga caataagaag gaaactgttg gtgactatga tatggttcca ttgatcgatt
      241 cattcattaa aaaacatccc gatttttctt atcaaggcgc caaaggaaca ttagctttaa
      301 caggctataa tggtgtatta ggttaccgta catcaaaatc agaatatggt gataacgaaa
      361 aaacacacaa agaaatcgaa gcagccaaaa aagttgctga tcaattaaag gaagatggtt
      421 ggagttttgc ttctcatact tggggacact tgaatatgac acaagcttct ttagccgata
      481 tccaacaaga taatgaacgt tggcaaaatg aagttgcacc tattttaggc aaaacaaata
      541 ttttgatcta cccatttggc gctgacatca gcgattggca accttattct gaagcaaatc
      601 aaaaatttgc ttacttgaaa caacaaggat ttgatatctt ctgcaatgtc gatgcctcaa
      661 ctcctgcatg gggacaacta ggaactgact actaccgtaa cgccagaatc aatattgacg
      721 gtatccgttt cgaagctgac ctaaaaggag aaaatccaat acttgatcac tttatcaacg
      781 tcaaagaaat gtatgaccaa aaagatcgag gatagaggtt gtgaaagaag cgtccagctc
      841 cgagcaaata agaactagat ttctaaaata gtttctcata ttttggaaat ctagcgctta
      901 ttgctgagga gttgcttctt gaacaccgtg aatagaggtt gtaaaaagag cgttttgact
      961 tattgttgaa ggtcagctct ttgaacaccg cgtgttaggt tgtgaaaagg ctggtctgca
     1021 tcaagcaata agaatgaaca gagaaaaaca gcttcttgaa taccgcagat aggttgtaaa
     1081 aaaaccgttt tgacctgacg ataaccagta aaacagaggt gagaccattg ctaaaagtag
     1141 cagtggtctc atctcttttt ttgcacaagt catatttgat tgtttcgtta ttttaatcag
     1201 gataaagttc tttttttcat gctaaagagt gattctttct tctcgatttt cactttcttc
     1261 gtttaacttt tggtaaatac ttctgtcaga aaaagctcta ggtagacaca cgaaagcaga
     1321 taaaaagcct aacttttgca acagtttatg ctgtcgttca aagttaggct tttacagttt
     1381 ataaata
//
LOCUS       DACEQH010000161         1364 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.170, whole genome shotgun sequence.
ACCESSION   DACEQH010000161 DACEQH010000000
VERSION     DACEQH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.170"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            80..>1364
                     /locus_tag="IW833_002797"
     CDS             80..>1364
                     /locus_tag="IW833_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ1308832.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI"
BASE COUNT          465 a          263 c          257 g          379 t
ORIGIN      
        1 tttaggtatt aaaaaacata tcgttctcta gtatgattaa atcaccacaa aataaacaac
       61 tgagaggacg atacgctata tgaatgattc tatcaaaaaa atgctgagaa taacagagaa
      121 agatttgatg attacagagg tctcttacga gacccttcag aagaaaaaga cgttggtcgt
      181 cgatgctgtt ctctcgccta ctcctcgtgc ttgtagaagt tgtggttcta ctgtggtaga
      241 tggaaacggg aaagcaatta tagtgaaaaa tggaaaaaag gaaacgattg tccgttttga
      301 acaatacaat catatgcctt tggttatgcg cctaaaaaag caacgctata cctgtaaaaa
      361 ctgtcgcacc cattggacgg ctcaaagtta ttttgtccaa cccagacatt caatcgcaaa
      421 tcatgttaga tataaaattg cttctttact gactgaaaaa gtatctttat cttttattgc
      481 gaaaagctgt caggtatctt tgaccaccgt tattcgtaca ttgaaagagt ttaaaagcta
      541 tttaccaaag caatctaaga agatcctccc cagagtattg atggttgacg aatttcgttc
      601 gcatgcttcc atagaagata agatgagctt tatttgcgcg gatggcgaaa caggaaaatt
      661 aatagatgtt ttgcctacgc gtaaattacc tcgattaaca agctatttct taggttgtac
      721 caatccagaa gaagtagaat tcttggtgac agacatgaac gccgcctact tccagctcac
      781 caaacgtgtt ctgccaaatg cgaaagtcgt gattgatcgg tttcatattg tcaaacacat
      841 gaatcaagcg ttcaatgagt tgcgtatccg tgaaatgaat gaacttcgta aagccggaca
      901 gaaaagccag gcagaaaaac tgaaaaagaa ctggcgcttt ttgctaaaaa atcgtgcaaa
      961 catcaaccat tatgaataca aaacatggaa aagtttccga gcaccaaaat acccatttct
     1021 tactgaagca atgatgattg atcgattgct tgagttttct ccgcccctaa aggaggcgta
     1081 tccctttttt catgaattag ttgaagcctt tcgagacaaa gaccctgact tatttttctc
     1141 cttattggca gaacttcccg aaacgttaga tgacagcttt cgggaaaagc ttcaaaacct
     1201 tctgacctat gaagaaggca tcaccaacgc aatgatctat ccttattcca atggaaaaat
     1261 agaagcgaag aatacccaca taaagacaat gaaacgagta tcctacggat ttaaatcatt
     1321 tgagaacatg agaattagaa tctttttgat caatcaatta atca
//
LOCUS       DACEQH010000162         1354 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.171, whole genome shotgun sequence.
ACCESSION   DACEQH010000162 DACEQH010000000
VERSION     DACEQH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.171"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            75..1325
                     /locus_tag="IW833_002798"
     CDS             75..1325
                     /locus_tag="IW833_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1308833.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT          427 a          210 c          235 g          482 t
ORIGIN      
        1 caattcgggg atatcgtcat caaattatta tgttaggaaa atctaattat cgttaattaa
       61 ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc tgcaaaaatt agtaatcaaa
      121 gacatctact agctttaaga gacagcatgg ctgcagttat gccattaatc attattggtt
      181 ccttttttat gttattagca aatttcccga tagaaggatt tacaaaatgg ttatctgaaa
      241 ttggagtaca atcctttttg aacaaagcat cagattcaac atttggtatt attggattag
      301 ctgttaccta ctctttggga cacaatctag caaagactta tggtgtagac tcattatcag
      361 caggattatt atctttgagt ggatatgttt tacttacacc tttattggat gcagatggtt
      421 ctataggatt tccaacaaaa tactttggta cctcgggtct ttttgttggt attattgtag
      481 cacttgtatc tacagaaatt ttccggtggt ttgtacttaa aaatattaca attaaaatgc
      541 cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc tattattcct ggcttttttg
      601 tcgttctact atggtttatt gttgttgtaa tttgttataa acttggaatt gaaaatgttc
      661 atgctcttat tgcagataca ctcgcaaaac cactatcttt actgacgaaa actttaccag
      721 gaattatatt ggttatactt attcaatgtt tcttttggat gtttggtatt catggtgcac
      781 aggtaacggg gccaataatt gaaccgttat tattgcaaaa ctctgatgta aatagaattg
      841 cttatcaagc tggaaaagaa cttcctaaca ttataactta tgaattctta tataactttg
      901 ttttttctgg tggtgcaggt tgtttatttg cacttgccat tttaacattt ttcttttcaa
      961 agagtcagga aaataaaact ttaggtaagt tatcaatcgc tccagtaagt tttcaagtag
     1021 cagagcccct tctatttggg tttccaacta tattaaatat aaaaatgtta attccatttg
     1081 taactgcacc agtagtaaca acattaatta cttatttttc aatgagtatg ggattagttg
     1141 caaaacctgt aggagcaaca atcccatgga caactccacc gataattgcc ggatttttag
     1201 cttctggtgg acgaataagt ggtgcagtga ttcaagtaat aacaattgca ataaatgttt
     1261 tgatatacta tccattcttt aaattagatg ataatgccaa gctaaaaagc gaaaaaaatg
     1321 attagaacta cctatatgtt tctaactgta gtta
//
LOCUS       DACEQH010000163         1337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.172, whole genome shotgun sequence.
ACCESSION   DACEQH010000163 DACEQH010000000
VERSION     DACEQH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.172"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1337
                     /locus_tag="IW833_002799"
     rRNA            <1..>1337
                     /locus_tag="IW833_002799"
                     /product="23S ribosomal RNA"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DACEQH010000164         1311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.173, whole genome shotgun sequence.
ACCESSION   DACEQH010000164 DACEQH010000000
VERSION     DACEQH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.173"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            24..716
                     /locus_tag="IW833_002800"
     CDS             24..716
                     /locus_tag="IW833_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ1308834.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
     gene            915..>1311
                     /locus_tag="IW833_002801"
     CDS             915..>1311
                     /locus_tag="IW833_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1308835.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEI"
BASE COUNT          447 a          247 c          256 g          361 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttatttt ttggctctat actttttgat gttggtgaac ttcaccaaca tcttttttgt
      841 ataaaaaaat atagaaaaga caccctgaaa tcctttagaa ttaatttggc taccaattca
      901 taggaggatt caagatgtct catactcagc ttatcaaaga taccctaaat attctagacc
      961 taaatatcta tttcgaggaa aattgtttaa ctaaagaaaa atacaaaggg caaatttgct
     1021 tgatttacaa aggaactctt ctgtacaagc cagaagagtg ttcccattgt ctttgtgtcg
     1081 tcccctctag gatcattcgt tgggggacga caaccgttcg gttgttactg aatgatgtgt
     1141 ctgagtatcg cacttatctg gagctgaaga aacaacgatt taaatgtaag tcttgccaaa
     1201 gaacattcgt agcggatacc tctgttgctg aaaaacactg tttcattagt caaaaagttc
     1261 gttggtctgt cattgctcgc ttaaaagaga atacttccat gacggaaatc g
//
LOCUS       DACEQH010000165         1305 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.174, whole genome shotgun sequence.
ACCESSION   DACEQH010000165 DACEQH010000000
VERSION     DACEQH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.174"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            88..>1305
                     /locus_tag="IW833_002802"
     CDS             88..>1305
                     /locus_tag="IW833_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1308836.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNA"
BASE COUNT          426 a          267 c          238 g          374 t
ORIGIN      
        1 cttttttatt ttccaaaaca tatagaaaag acaccttgaa atcctttaga attaatttgg
       61 cgaaccaacc caaaaggagg aatcaagatg tcttacactc accttatcaa agaaacatta
      121 gatattctag acttatcagt tacttttaac gaaaattgtc tcacaaaaga aaaatacaaa
      181 gggcaaatct gtcatattta tcgtggaaac ttgatttata cagcccaaga atgcatccac
      241 tgtaaacacc agattgcttc agatattgtg cgttggggta caacaaccgt ccggttgtta
      301 atgaacgatg tttctgaata ccgtacctac ctcgaattga aaaagcaacg cttcaaatgc
      361 aaagcttgtc agcggacctt tgtcgcagat acttctgttg cgaaaaaaca ttgtttcatc
      421 agtgagaaag tccgctggtc tgtcgtgact cgtctgaaaa aaaatacctc aatgacagag
      481 atcgccgctc agaaaaatct atcggtctct tctgtttacc gtattatgaa gcgattttat
      541 cgtccgttga atccttttcg gacaccgtta cctaaggtac tttgttttga tgaattcaaa
      601 tcagttcggg gtgtttcagg tgctatgagt ttcatcatga tggatggaca aacacagcgc
      661 ttattggaca ttgtcgagaa ccgacaactg ccttttttaa aacgttattt tagtcatttt
      721 tcacgagaaa ttcgtgaagc ggtcgaatgg atcgtgattg atatgtacgc tccttatgta
      781 tcgctcgtta aaaagctgtt tccaaaagca cagctgatta ttgaccggtt tcacatcgtc
      841 caacatattg gtcgtacctt tcgcaatcat cgaatcaaag aaacgaacca actattaaaa
      901 agcaaagaac aaaaacacta tcaattaggt aagcaattaa aaagatactg gaaacttctt
      961 caaaaagatg aacgcaagct tgattatact cgacgccttt ggcgacctgg cttcaaagct
     1021 catttaacag agaccgatat tgtggatcgc ttactgaaag ggagccctgc tttgcgtgta
     1081 ggctatcagt tatatcaaga ctttctttac gctgtaaagg aacgagacta cgtatcgttt
     1141 gaagaactac ttaccaataa tatcatgctt cccgaaggct atcagacgac cttgagaacg
     1201 ttccagaaat ttctgccaca aatcaaaaat gctttacaac aaagttattc aaatggccca
     1261 cttgaatgtc tgaacaacca tatcaaagtg ttaaaacgaa atgca
//
LOCUS       DACEQH010000166         1296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.175, whole genome shotgun sequence.
ACCESSION   DACEQH010000166 DACEQH010000000
VERSION     DACEQH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.175"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..820
                     /locus_tag="IW833_002803"
     CDS             <1..820
                     /locus_tag="IW833_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1308837.1"
                     /translation="GVGKSTCAQMLGFESAVNEGKRTLIIDLDMQGNTSDVMNLTHMN
                     FTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPADMSFEMYDDWIKD
                     QYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIADWAIVVLQTQVKS
                     MRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQAQEIYGEHLIKNV
                     VLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEEHENWYAEVE"
     gene            821..>1296
                     /locus_tag="IW833_002804"
     CDS             821..>1296
                     /locus_tag="IW833_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308838.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRT"
BASE COUNT          489 a          193 c          273 g          341 t
ORIGIN      
        1 tggcgtaggt aaaagtacgt gcgcgcaaat gttagggttt gaatcagcag ttaatgaagg
       61 aaaaagaacc ttgataattg acttggatat gcaaggaaat acttctgatg tgatgaactt
      121 aacgcatatg aacttcacta acgaagaagg aggcggagaa ggtgaaccga ttgtttacga
      181 aaatactgtg actgacgtgt tgataaacaa tcttccacct aaagaggctg tctataaagt
      241 aatcaataac ctctatatac ttccagcaga tatgtccttt gaaatgtatg atgattggat
      301 caaagaccaa tataatgatt cattatccca attcaaatat atgaaagaaa agctagaacc
      361 attatttgat gagtttgacg tcatctattt agatgtccca ccttctattt ctgtatatag
      421 taaatccgcg atgtacattg cggactgggc aatcgtcgtc ttacagaccc aagtaaaatc
      481 gatgagaaat gcgatgcaat atcttgagta tatggacttt tttgtgaaag aatttgatac
      541 aaatttatat gttgctggtg ttattccttt catgttggaa agcggtgacg cagtagatca
      601 ggaaatgtac aaacaagccc aagagattta tggggaacat ttgattaaaa atgttgtact
      661 caaaaatgca cgcttaaaac gatacgacgg atcaggtatt acgatggaga agacaaagaa
      721 aggtctctta aaacaatggg atcgcaaaag ccacgaatta tttatcagta ttttgaaaga
      781 attggaagaa cacgagaatt ggtatgcgga ggtggagtaa ttggctgaca tcaaaggact
      841 tgatattttt gggaacaaca aaggactcgc caaaataaaa aaagaagcag aagcaaagaa
      901 aaagcagcaa gcaattacac catctgaaaa taagcaatta gaagtgcacc aagttcaaga
      961 aataaaaact tcctccaatg ttgaaagaca gagtagagga agaacaaaaa aagttggcgc
     1021 accaatcaga aagtttgata aggtttattc caccaagcat cctttaaaac tgtcgacttt
     1081 gttaaatgcg acttccagag ttttagtaga aaagtatgag actaatatga caagagatga
     1141 attgcttaga aaagctttag atgagtatat taaacagaat cttacgcaag aagataagca
     1201 agatttgttt aatgacgtgg tgaaagaact agacttgttc agagaaaaat atccaattgt
     1261 tccagaatta gatgaccgtg gagaaataat aagaac
//
LOCUS       DACEQH010000167         1294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.176, whole genome shotgun sequence.
ACCESSION   DACEQH010000167 DACEQH010000000
VERSION     DACEQH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.176"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1244
                     /locus_tag="IW833_002805"
     CDS             <1..1244
                     /locus_tag="IW833_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1308839.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DACEQH010000168         1288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.177, whole genome shotgun sequence.
ACCESSION   DACEQH010000168 DACEQH010000000
VERSION     DACEQH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.177"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(567..911)
                     /locus_tag="IW833_002806"
     CDS             complement(567..911)
                     /locus_tag="IW833_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308840.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(1008..1253)
                     /locus_tag="IW833_002807"
     CDS             complement(1008..1253)
                     /locus_tag="IW833_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1308841.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
BASE COUNT          439 a          223 c          246 g          380 t
ORIGIN      
        1 ttattaattt tcgtctgaaa acaaaaaaag aaagcgttat tattttagtc tattttcagg
       61 gggggaacct tatgataaaa aaactatttt tactatcaat attaacgcta gcaatggcag
      121 gttgttctga ctctggctta gcaactgaac tgatcacaaa atcaactgaa gccacgacag
      181 aggagttaaa atgtggaaac gatcacagtg actacagagg cttaaatcga cgatgcatgg
      241 gaatattagg cagaagcaat tgtaagcatc gatgtagtaa tcgaagaagg tgtagaagaa
      301 gcaacagaaa aatcaaaaga aagatcaaaa gaagcaatct gtgctgtgca tcgtatacag
      361 acgtcgaaga tgcggtactt ttaaaccacg tcaggagatg ttcatttatg tctagggaag
      421 ttggcgatga accaaaacta gagtacgctt ttagttttgc acgtgaggat gaagataacc
      481 ctaaaactta cgacatttta gcttgatcta agatggttaa agctttttaa tgaagtaaga
      541 ggagatagaa ggcttttcta gttatattag gtaagataaa ttcctttttg ctccaaattt
      601 gctaaacatt ctgctagata aacttcatcg tgttccggat aaatcggcac gttgcgctct
      661 acagtaggta aatcaaaaaa tgcttcgcat gatttgccac gatacatagg atttttccac
      721 tctggtgctg ggctgtaacc acgtttttcc atttcatcca tgacaagctg atgatattgg
      781 aataatctat aaggagaata gtgaaaaaca taatccacag tagcatgttt ctttccccag
      841 ccatttcctc ttaatgcaca gcattcccga tgttgtccta aaagctgttg acgaggaagc
      901 tttgaaatca atttttcatg ccataatcac atagaaatgt ctcctttata tatagattgt
      961 taaccttaga cgttaaatga aaaaaccagc accaaggctg gaaaaaatca cttcagtttg
     1021 cttgctgttt ctgagcgacc aattaggtaa tcaacggaaa tatcgtaaaa gtcagcgata
     1081 tagattaatt caatgatgct tggaatttct tctccttctt cgtacttacg atatttgaat
     1141 aaaggaatac ttaacgcgct agaaacagct acttgagata aagatcggcc agttcttaag
     1201 aaattcagac gacaacgtaa agtatccagt aaaatagctt cttcgatact catttaatca
     1261 cctttcatta tagtagaata gttataat
//
LOCUS       DACEQH010000169         1277 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.178, whole genome shotgun sequence.
ACCESSION   DACEQH010000169 DACEQH010000000
VERSION     DACEQH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.178"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            82..1251
                     /locus_tag="IW833_002808"
     CDS             82..1251
                     /locus_tag="IW833_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1308842.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          460 a          206 c          233 g          378 t
ORIGIN      
        1 aatagaaatt tagggtagaa aaaaaagtcc attctgtaaa attaaagtta ccacacagaa
       61 attttatagg aggactttct catgactaaa tttactatag aaattatgca aacactactc
      121 aataaagggg atttagatga attatttcag gatcatttag agcgagcgat taattcactt
      181 ctacaagcag agctaactgc ttttttagat tacgaaaaat atgatcgaaa tggattcaat
      241 tcaggaaatt cccgtaacgg gaattattca cgtacattta aaacagaata tggcgagttg
      301 aatttaacta ttcctagaga tcggaatggc gaattttctc aacaaacact accagcatat
      361 aagcgaacca atgattcatt agaaacgacc attatccaat tatttcaaaa aggcattact
      421 atggctgaaa tctccaaatt aattgaaaaa atgtatggtc accactatac gccacaaaca
      481 atctccaaca tgagtaaatt ggtggctgaa gatgttttag cttttaaaga aagaacgtta
      541 gaagccaatt attccgttat atttatggat gcgacgcata tccctgtgaa gcgacagact
      601 gtttcgaagg aagcagtgta tattacgatt ggtattcgtt tggatggaac caaagaagtt
      661 cttgggttta ctattgcccc aactgagtct gcttatattt ggaaagaagt tcttcaagat
      721 cttagaaaac gtgggttaga agaagtttta ttagtagtga cagacggatt aagctgtatt
      781 gaagaaagta tccatagtgt gtatccgaat gcccaatttc aacaatgttg tgtgcatgta
      841 tctagaaata tcgctcataa agttcgtgtt cgaggtcgaa aagaaatttg tgaggatttc
      901 aaattggttt accaagcgaa ttcaaaagaa gaggcattgg atcacatcga ctttatgact
      961 aggaaatgga aaaagcagta tccaagagtc gtcaatttac tcttgaatcc tgccctatta
     1021 accttttata atttccctca cgccatcaga cgaacaattt attcgacgaa cctgattgaa
     1081 ggctttaata agcagctaaa acgatatact cgaagaaaag aacaattccc taatgaagaa
     1141 tctctagaga gattcttcat ttctcaattt aatcaatata accaaaaatt tttaggtaga
     1201 attcacaagg gatttaaaga aattcaggat acattagagt cgatgattta actgtaatta
     1261 acagaatgga ttttcca
//
LOCUS       DACEQH010000170         1267 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.179, whole genome shotgun sequence.
ACCESSION   DACEQH010000170 DACEQH010000000
VERSION     DACEQH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.179"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            66..1244
                     /locus_tag="IW833_002809"
     CDS             66..1244
                     /locus_tag="IW833_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HAQ1308843.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDIYSRG
                     VHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRNIAHKVRVKDRKEICDDFKAV
                     YQASSKEEANTFLGSMIEKWQKTYPKVTQSLIKNQDLLTFYEFPPGIRRSIYSTNLIE
                     SFNKQIKKYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
BASE COUNT          427 a          254 c          246 g          340 t
ORIGIN      
        1 aaaaaggaag tcccttctgt agaatagagt taccacaaca cattcacaga aaagaggact
       61 tccatatgaa cgattttact acagaaattc taaagactct agcgaacaaa ggcgatttga
      121 atgaattatt ccgtgtccat ttggaaaaag ctgtcaatac gcttctcaaa acggagttaa
      181 cggctttcct cgattacgaa aagtacgatc gcattggttt taacacgggt aattctcgta
      241 acggctccta tgaccgtacg gtcaagaccg agtacgggga acttcatctc cagattccgc
      301 gcgaccgcaa cggcgagttc aagcaacaga ctgttcctgc ttatagacgg acgaatgata
      361 ccttagagga gaccgtcatt cacctcttcc gaaaaggtat taccatgtcg gaaatcgcag
      421 acttgattga gaaaatgtat gggcatcact acacgcccca aaccatgtcc aacatgacta
      481 aagtgctgac tgaagaagta aatgcattta aagttagaac tctaaatgat aagtatgtcg
      541 ctattttcat ggatgctact tatattcctt taaaacggca aaccgtctcc aaagaagcaa
      601 tctatattgc cattggtata cgagaagacg gcactaaaga agtactaagt tatgcgattg
      661 ccccgactga atcgacctac gtttggaatg agttgttaca ggacatttac tccagagggg
      721 ttcatgatgt cttactgttt attaccgatg gcttaaaagg catgaaagat acgattcacc
      781 agatttatcc taaagctaaa taccaacatt gctgtgttca cgtttcccgt aatattgctc
      841 ataaagtacg tgtcaaagat cgaaaagaaa tctgtgatga ttttaaggct gtttatcaag
      901 ctagctcaaa ggaagaggca aatacctttt tagggagtat gattgagaag tggcagaaaa
      961 cttatcctaa agtgacgcag tcactgataa aaaatcaaga tttattgact ttttatgagt
     1021 ttccgcctgg tatccgccga agcatttact caactaattt aatcgaatct ttcaataaac
     1081 aaatcaagaa atacagccgc agaaaagagc agtttcaaaa tgaagaatca ctagagcgtt
     1141 tcctagtatc catctttgat acatacaatc aaaaattctt aaatagaagc cataaaggct
     1201 tccaacaagt gacggataca ttagcttcaa tgtttactga gtaacccatt attttgcaga
     1261 aggacga
//
LOCUS       DACEQH010000171         1263 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.180, whole genome shotgun sequence.
ACCESSION   DACEQH010000171 DACEQH010000000
VERSION     DACEQH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.180"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            35..>1263
                     /locus_tag="IW833_002810"
     CDS             35..>1263
                     /locus_tag="IW833_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1308844.1"
                     /translation="MLNAYQPESVDDMQDALKDVFGPLFEKMLQGELNNHLGYDAHSK
                     EPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIPKRKRDVSDIEGKVLSM
                     YARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQARPLAKCYAFLFVDCM
                     YVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNRWMQVFDELKARGVEDV
                     FFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSKDYKEVCRDMKKFYGAS
                     SLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSAIRKIMYTTNAVESVHS
                     SFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAMVLNQLMINETFSSRMK
                     SMRFTFRNNGRFIQLCVR"
BASE COUNT          383 a          237 c          273 g          370 t
ORIGIN      
        1 aagaaatcct gatgccgaaa agttagctga atccattctg aatgcctatc aacctgaatc
       61 tgtcgatgac atgcaagatg ctttgaaaga tgtgtttggg cccctttttg aaaaaatgct
      121 tcaaggagaa ttgaataatc atttaggtta tgatgcccat tctaaagagc ctaaggaaca
      181 cgataaccgt cgaaatggct atggaactaa aacgcttaaa accagttttg gtgaagtagc
      241 tattgatgtt cctagagacc gggaagcttc ctttgaacca gagttaattc ctaagagaaa
      301 acgagatgtc tccgacatcg aagggaaggt tctttccatg tatgcacgag gaatgagtca
      361 acgagatatt gccgcaaccg tcgaagctat ttatggcttt gatatttccc atgaaatgat
      421 ttcagatatc actgacgctg tccttcctga attggaagaa tggcaagccc gccccctagc
      481 aaagtgttat gcctttctat tcgttgattg tatgtatgtt actttacgag aaaattatga
      541 agccaaagaa tatgctgtat acaccattct tggctatgat ctcaaaggaa ataaggagat
      601 tttaggatta tggttaaatc aaacagaatc taaaaatcgc tggatgcaag tatttgatga
      661 actgaaagca cgtggtgtcg aagatgtctt tttcatttcg atggacggtg tttctggcct
      721 cgaagaaggc gcaaaagcga tctttccatc ggtaattgtt cagcgctgta ttgttcatct
      781 tgttcgaaat gcattgcgct atattccaag taaggactat aaggaagtct gccgagatat
      841 gaaaaagttc tacggtgctt cttctctaaa cgctgcacat gctgcttttg acagttttca
      901 aaatcggtgg tctcattatt ctggtgctgt agatgtctgg aaacggaact ttgcacatgt
      961 tgaacaacta tttgattatg gtagtgccat tcgaaaaatt atgtacacta ccaatgcggt
     1021 tgaaagtgtc cactccagct tcagaaaagt cacaaaaaaa ggagcgttct ctaatgagaa
     1081 tgcactccta aaactacttt atttacgcac gaaagaatta cacgcgaagt ggtcaggtgg
     1141 tcgaattcaa aactgggcta tggtgcttaa tcagttaatg atcaacgaga ccttctcctc
     1201 tagaatgaaa agtatgcgat ttaccttccg taacaacggt aggtttattc aactatgtgt
     1261 ccg
//
LOCUS       DACEQH010000172         1251 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.181, whole genome shotgun sequence.
ACCESSION   DACEQH010000172 DACEQH010000000
VERSION     DACEQH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.181"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            20..1165
                     /gene="tnpB"
                     /locus_tag="IW833_002811"
     CDS             20..1165
                     /gene="tnpB"
                     /locus_tag="IW833_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1308845.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
BASE COUNT          480 a          195 c          217 g          359 t
ORIGIN      
        1 agaaatgagg tgaatccgta tgctacaaca tagggcttat aaatttagaa tctatcctaa
       61 tcaagaacaa gaaatattaa tgactaaaac tattggctgt tcaagatttg tctataatta
      121 ttttttgaat ttgtggaatc atgaatatac gactactgga aaaggattat cttataattc
      181 ttgttctgcc atgcttccac aaatgaaaag aaatgcagag acaatttggt taaaagaagt
      241 tgacagtatt gcgattcaat cctcattgaa aaatctttct gatgcttttt ctcgcttctt
      301 taaaaagcaa aataaacgac cacaatttaa aagtaaaaaa catcctatac aaagttatac
      361 aactaaaaat gtgaataata gtatcgaaat tagaaacaat ttcgtaaaac tacctaagtt
      421 aggatttgtt aaatttgcga aaagtcaaga gcccaaaggg cgaatcttaa atgctacgat
      481 tcgaaaaaat tcgagtggta aattttttgt ttccatcctg tgtgaagaag aaatagttga
      541 gtttcctaaa tctaattccg ctattggaat cgaccttggt ataactgatt ttgcaatcct
      601 ttctgatggt caaaagattg ataacaataa attcacgtca aaaatggcaa aaaaactcaa
      661 acgtgaacaa cgtaagttgt caaaacgtgc attactagct aaaaagaaag gcgtcaatct
      721 ctttgaagct aaaaattatc aaaaacaaaa acgaaaagta gcacgactac atgaaaaagt
      781 aatgaatcaa cgcaatgact ttttaaacaa gttgagcaca gagattatca aaaaccacga
      841 tattatctgt atagaagact taaatacaaa aggtatgttg cgtaatcata aactagcaaa
      901 aagcatttct gatgtgtctt ggtctagttt tgtgactaag ttacaataca aagctaactg
      961 gtacggtcgg aaaatcatca aagtagataa atggtttcca tctagtcaaa tctgttcaaa
     1021 ttgtggacac aaagacggca agaaatctct agaaattcga gaatggactt gtcctgtttg
     1081 tcatgctcat catgaccgag atatcaatgc tagtatcaat attttgaccg aaggtctaag
     1141 attacatgtc atgggattag cttagatcca atcatcaaaa accgtaggaa ctacggggat
     1201 agcttggtaa ataagagaaa ccgctgtgag taaagaaata cgcttgcaag t
//
LOCUS       DACEQH010000173         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.182, whole genome shotgun sequence.
ACCESSION   DACEQH010000173 DACEQH010000000
VERSION     DACEQH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.182"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1076
                     /locus_tag="IW833_002812"
     CDS             <1..1076
                     /locus_tag="IW833_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1308846.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACEQH010000174         1158 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.183, whole genome shotgun sequence.
ACCESSION   DACEQH010000174 DACEQH010000000
VERSION     DACEQH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.183"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(231..410)
                     /locus_tag="IW833_002813"
     CDS             complement(231..410)
                     /locus_tag="IW833_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308847.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            complement(791..>1158)
                     /locus_tag="IW833_002814"
     CDS             complement(791..>1158)
                     /locus_tag="IW833_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308848.1"
                     /translation="YTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTRF
                     LTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQ
                     NELDRKQMPYQRFERKKGQ"
BASE COUNT          358 a          224 c          182 g          394 t
ORIGIN      
        1 tatgttaaca tttatatgaa aatagtcgaa aggaaatcat aatatgttta agaaaacaaa
       61 atctttagaa tcaggtaaga aaaaaattga tctaaaaata atctcttctg gatactttaa
      121 agatttcata gttattgata caaaatggaa taaaaaggac atagaaaata ttctaaatag
      181 taggaacaat taagtgctat cacttttgtt tcttcaaatg aaaagtgacg tcactttttt
      241 agtgctggta tatcaaatcc tgctttttct tgcgcgatca atagatctaa cgcaatacac
      301 ataatcttag attcactttt tccgtacttc tgctttagtt gctgcaattc tttttgtctc
      361 ttttcatcta atgtcacggg taaccgttta tttcctattg caattggcat tagtactctc
      421 tcctttcatc gaaataaccc tatgaaaaat atggctgcaa tcaacttgaa tattagactc
      481 acaagggcta acacaaagcg ccaaaagatc aaacaaaata tcagtattcc taagaatact
      541 acgaagttta aatgtacagc tgcaccatga cgcataagta tctcgccaat acacagtacg
      601 aacaacagag gaatgatttt attcatatgg atctccttct ttctcttagg ttatcgggct
      661 ttgcccgctt atttcgcacc acctaagcgg cgggggcggt tggagcgatt ggtgcgaaat
      721 aagaaccatt cccttaattt caaggttttt tgttcttatt tcacacattc tcacttttcg
      781 gtataaatga ttattgacct tttttccttt caaatcgttg atatggcatt tgtttacggt
      841 caagctcatt ttggatttct tctgtatcgt cgtaagtgta ggtgtctaag aaatcagtta
      901 aagtcgtgta ccaatcatcc cctgggttat ctacttcttc tacatattcc ctcaaaaacg
      961 cttcgggtac agcgagttcg taatccattc ccattagcac gatagattcc gttccacacg
     1021 ttaaaaatct agtaaataca aggtaataat cgtctaccgt cgcatcttta ctgtttaact
     1081 cttccattaa ttccatgctc tcaaaaattg aataatattc tgattctgag taatagtgtt
     1141 tcctgcaaat cgtgtagc
//
LOCUS       DACEQH010000175         1082 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.184, whole genome shotgun sequence.
ACCESSION   DACEQH010000175 DACEQH010000000
VERSION     DACEQH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.184"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            700..>1082
                     /locus_tag="IW833_002815"
     CDS             700..>1082
                     /locus_tag="IW833_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1308849.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMW"
BASE COUNT          413 a          161 c          244 g          264 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 ga
//
LOCUS       DACEQH010000176         1041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.185, whole genome shotgun sequence.
ACCESSION   DACEQH010000176 DACEQH010000000
VERSION     DACEQH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.185"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            191..445
                     /locus_tag="IW833_002816"
     CDS             191..445
                     /locus_tag="IW833_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1308850.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IW833_002817"
     CDS             446..763
                     /locus_tag="IW833_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1308851.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          366 a          164 c          213 g          298 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt t
//
LOCUS       DACEQH010000177         1020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.186, whole genome shotgun sequence.
ACCESSION   DACEQH010000177 DACEQH010000000
VERSION     DACEQH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.186"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            73..>1020
                     /locus_tag="IW833_002818"
     CDS             73..>1020
                     /locus_tag="IW833_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1308852.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLS"
BASE COUNT          353 a          175 c          189 g          303 t
ORIGIN      
        1 agacaactaa aaaagtcatt tgtgctacac tcaaaatagt tccgaccaaa gaattataag
       61 gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat aatagagtct
      121 tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg ttcaagacaa
      181 acgatccata aagtatatct gttcttcaag caaggaaaat cagccttaga atattatcaa
      241 cagtataaga aaaacaaatc aaactgtggt agacgtccgc ttgttttacc cgaggaacaa
      301 tcagaatata ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat tgttggtcgt
      361 gcagcgtttc ctattagttg ttctgctcgt accatttatc gtatgttcaa aaaggggcta
      421 tttgattctt ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca tcaagaaaga
      481 cgtggaaaac aaactttccg ccgctctatt catgaacgtg aaaaggatta tagccaattc
      541 tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtctgaaaca taaaagtgct
      601 gtaattacct tagttgaacg attatcaaaa gttatcatca cattgaaacc gtgtggtaga
      661 caagcgattg atattgaaaa aaaattaaat caatggtttg aatctgtacc gaaaaaccta
      721 ttcaaatcca tcacttttga ttgtggaaag gaattttcaa attggaaaca gatcagtaat
      781 gtcaatgata ttgccattta tttcgctgat ccaggaacgc cgtctcaaag aggcctaaac
      841 gagaattcta acggattgtt acgtagagat ggtttattga aatctatgga tttcaattca
      901 gtagatgaat tttttattca atctgtcgca tctaaacgaa ataatattcc tagaaaatca
      961 ctggattatc gaacaccttt ggaagtattt ttgagttacg taagtattga tgatctgtct
//
LOCUS       DACEQH010000178         1010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.187, whole genome shotgun sequence.
ACCESSION   DACEQH010000178 DACEQH010000000
VERSION     DACEQH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.187"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(51..704)
                     /locus_tag="IW833_002819"
     CDS             complement(51..704)
                     /locus_tag="IW833_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ1308853.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT          312 a          220 c          140 g          338 t
ORIGIN      
        1 aaaaagactg cgacacaatt tttgtcacag tctcttacta taatgctgat ttatacaaaa
       61 taaaatttcg ttcgctcgat agcattacgc gccatcagac attgtccata ttcattcaat
      121 gtttctggtg tacgatcgct cttttcagca aactccaaaa ttcgagcaat ctgattctct
      181 acatccgctt ggttgagatt ttccatccag aagtgaacga ctaggtaata gcgctcttcg
      241 aataaataca aatcagtcca agcagactct agttcgacat ctgttgcaag ttggatcact
      301 tgttcaaaat cttcaaaagc aaaagttgct tgtctttcct gttcttctag ggcgcttcca
      361 gataattctt cctccggatc gctgccaata atctgctgac gaagaaagtc actcacatct
      421 tctgaatcaa cagtagaatc ttctgattga gaaatttctt catccggaag atttttactg
      481 ataaacagtt ctaatccgtc tccttttggc aaaacttgga aagtgacagc ttcgcttcct
      541 cggaactcct cttcgatatc aacttcttcc aaaatactat aaaagaaatt ttcgatttct
      601 ttgtgattgc caagtaaatc caaaaatgaa acgccccgtt cctctaaatc ttcatgacca
      661 attagtacac ggatcgtatt ttcattaata tgttccattt ccattttgct acacctcact
      721 ttgccacaat gtttaatata gattcattgt agcgaaatca gcttaaatgt aaagaaaaaa
      781 aatctcgctg ttaagcgaat ttaataaaac cagcggattt ttcaaccttt caagctatct
      841 tatttaacta taacattttt ggcagacgaa tactatatag ccgacttttc agctaatttt
      901 ccctctctat ttttcgtgca atcatcatct tctactacct ttttatacat ctaatcaaga
      961 atactttttg taaaaacaaa aaaacttgga aaaactatct ttgtaatagt
//
LOCUS       DACEQH010000179          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.188, whole genome shotgun sequence.
ACCESSION   DACEQH010000179 DACEQH010000000
VERSION     DACEQH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.188"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>994)
                     /locus_tag="IW833_002820"
     rRNA            complement(<1..>994)
                     /locus_tag="IW833_002820"
                     /product="16S ribosomal RNA"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DACEQH010000180          954 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.189, whole genome shotgun sequence.
ACCESSION   DACEQH010000180 DACEQH010000000
VERSION     DACEQH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.189"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            233..475
                     /locus_tag="IW833_002821"
     CDS             233..475
                     /locus_tag="IW833_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAQ1308854.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            472..942
                     /locus_tag="IW833_002822"
     CDS             472..942
                     /locus_tag="IW833_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1308855.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
BASE COUNT          353 a          108 c          178 g          315 t
ORIGIN      
        1 agaaattgat tgtcgttttc ttttgcagaa atttaagtgc aggcaattat ggtagttgtc
       61 gtaattgctg tcagctcagt gtttatcact gatggtgtga aagatttacg gttggagatg
      121 ttcaataaga ttattggtgc ttaattattt ttattgtagt gctgatatga gaatgttata
      181 attggggtat aaaacataca aataaatttg ctgaaaaatg aggtatcaaa atatggaaaa
      241 tgaattactg gaaaacttag gaagacttca cacaactgaa cttggcgtta ttagaattaa
      301 aaagaactta tctttgaatg tagaaaatgt aattgaatgg tgcaaagaaa aaattagcct
      361 ttctaatgcg gaaatcacaa gaaaaggaaa aaactggtat ataacgattg ataattgtat
      421 tataacggta aatgcgtaca gttataccgt tatcactgct cacaaggtaa aatgataaat
      481 ttttctataa aggaattaaa cgcatttgca gttattgggc aggaagtaga actgactaat
      541 tatcaaaaaa agaatattca gattagtaca cagttttgga gaaagtttaa cagtagtttg
      601 aaaaaagcgt atctttcaca atcaggaaat tgggtaaaat atgcttttat ggaaagaaga
      661 aatggaaaac tttattattt ctgttctatt ccccaaagaa ccattacccc agagggcttt
      721 ctgtataaag aaataccgtc ttataaatat ttggttgtgg agcatattgg tgctatggat
      781 aaaatatatg aaacttatgg caagatttat gaagaaatta ttcctagtac atcgtatatt
      841 cctataaaag atattatcct acactttgaa aaatacgatt atcgttttca ttggaatagt
      901 gataattcag ttattgaaat ttggatacct attcaagatt agaagcaatt ttat
//
LOCUS       DACEQH010000181          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.190, whole genome shotgun sequence.
ACCESSION   DACEQH010000181 DACEQH010000000
VERSION     DACEQH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.190"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            2..>943
                     /locus_tag="IW833_002823"
     rRNA            2..>943
                     /locus_tag="IW833_002823"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACEQH010000182          919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.191, whole genome shotgun sequence.
ACCESSION   DACEQH010000182 DACEQH010000000
VERSION     DACEQH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.191"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            38..589
                     /locus_tag="IW833_002824"
     CDS             38..589
                     /locus_tag="IW833_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAQ1308856.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
BASE COUNT          354 a          127 c          180 g          258 t
ORIGIN      
        1 gagcgagcaa gacaggaacg agagaacaaa catactcatg aaaagactag aggcttcgac
       61 ctcgaacttt aataataagt tagatgtttt tgaagaaaag actaagcatg caagattaaa
      121 ctttgaaaca actgcaaagc actatattaa acgacttgat gaagataatt tgaaattaga
      181 ttttcaacaa gctattcaag atgaattaag cgatactaaa gacgaaatac gtgaagtgac
      241 gaaacaagcg agagaagaaa caaaagagta taaagaaatc ttagaaagta aaataaaaga
      301 ccataacaaa gtggttgata aatcgaatac agcgctaaaa gtgatgacaa aaggcgttac
      361 aaatatattt tttgtgctaa ttatttttgt acttgtaatg cttgtgacag gtccgatagg
      421 tcatttcttt ggtattgaac atttatatag ctttattaat ggttttattg atgaccacga
      481 aagtgcatgg cgatatttga tgttgatact ttatgcagta ccctatgtat tcttcgctat
      541 cattctgtgg ctcatagcat tgttttttag agtatttaat gacctttagg tagatttata
      601 tataaaaagg gctaaatacg caaaataccc ccttaaaatg caaaataaat gcaaataagg
      661 gggtaactcc ttatgaaaaa acaactaata gtattataac acgtttttat gccgagaaaa
      721 cttattggtt ggaatgggct atgtgttagc taacttgtta gcgagttggt tggacttgaa
      781 ttgggattaa tcccaagaaa gtaccaactc aacaacacat aaagccctgt aggttccgac
      841 caataaggaa attggaataa agcaacgaaa ggagttgagt aaaatgagtg agaaaacgaa
      901 gaaaatcatt cgagtatca
//
LOCUS       DACEQH010000183          915 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.192, whole genome shotgun sequence.
ACCESSION   DACEQH010000183 DACEQH010000000
VERSION     DACEQH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.192"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            183..884
                     /locus_tag="IW833_002825"
     CDS             183..884
                     /locus_tag="IW833_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1308857.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          332 a          161 c          147 g          275 t
ORIGIN      
        1 tttatacaca aatcattcgc ttcaaaacaa attattcaat accattgtat attttttaca
       61 taataacaaa ttatatttgt aaagtattaa aacttgtttc taatgttggt atgttaaaac
      121 tttacaaatt ctttaaaaaa agtgacaata aatatagtga acaataagga gaggactttt
      181 taatgcgtgt taataaaatc gattttcact tttcaaaact cagatatcaa cctgattttg
      241 aacatttctc ggatgaagag ttccaacaga tcaaggaaca tactgttctt cgttcttata
      301 aaaaaggaca gatccttttc gacgatggtg atcccagaca acggttgtat atcttgacca
      361 aaggactcgt tcgtttagaa cgttatgatg aaagtgcaac ttattactac tatgactatg
      421 tgaatacaaa taaccttttt cctatgtcag gaatgtttgg agaagagacc tacacttata
      481 cagctcaagc gatgactgat attgaaacat tctaccttcc tgtttcctac tatgaaaaat
      541 tagcacgaaa aaatcccgaa cagttgatct atatgataca aaaaatttct aaagtagttg
      601 aaatacatga attgcgtatc caaatcggat tgacctctag cgcctttgac cgggtaaaac
      661 aaaatttatt cattttgaaa gaagaattgg gcgtaccaac aaaagatggt catatcacca
      721 ttccttatcc gatcacctta aaagaattag cagtgaatag cggcacgaca cgtgaaactg
      781 ctggacaagt aatcaaacaa ctacgtgatt ccgggctgtt agattatgac aaaaagcgtt
      841 ttactttctg taagacagct actactccag ctatgagaga ataacgcgaa caaaaataag
      901 cagtgaccct gataa
//
LOCUS       DACEQH010000184          900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.193, whole genome shotgun sequence.
ACCESSION   DACEQH010000184 DACEQH010000000
VERSION     DACEQH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.193"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(221..294)
                     /locus_tag="IW833_002826"
     tRNA            complement(221..294)
                     /locus_tag="IW833_002826"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(258..260),aa:Ile,seq:cat)
     gene            complement(484..>900)
                     /locus_tag="IW833_002827"
     CDS             complement(484..>900)
                     /locus_tag="IW833_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1308858.1"
                     /translation="TSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVAG
                     WHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPS
                     KKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          281 a          176 c          156 g          287 t
ORIGIN      
        1 attattatct cacaatttaa cccaaatgtc tttctattta aaaatcaaag taaaactttt
       61 caaatataca gaagtataac tatgtgaaac atcctttcat taatccataa aaggatacat
      121 aaaaaagcca ctcatttgag tggcaatgaa gaaaagcttt agcttgtata atactcttca
      181 aaaaaattct aacacagaac gattcaaatg gctacgttaa tgtaccctgt aggactcgaa
      241 cctacgaccg gacggttatg agccgtctgc tctgaccaac tgagctaagg gtactggttg
      301 ttgccacata aagccataaa caatcaacca gtagaatgtg tggcaacaaa cctgttatcg
      361 catagcttgg agtgtgacta tttatgggtg atagtgaaga tatgcgataa catcactatt
      421 ttatcgaata atttttatag ttgtcaatat agttatgtac tgctcctcaa cgaggagcta
      481 tttttatcgt ttaggaatat ttaaatacca acgtttgtca tggaaatctt gcgcaccgcc
      541 tttagtgttt ccctctggat cattcgttgc ccgcatcatt acatagactt tcttactagg
      601 aaaattacgc atattgaaag atacatgata accaacattt ccagaagtat tataagcttg
      661 gtttacatct ggtctataaa ttccatcagc tcttactcga gctaattctt tcccagtatt
      721 gtagtccata atgaaaatat actcgtattt atagttagca atgtgccatc cagccacatg
      781 caagtttgcg ttttcgattt ccccaaactg atcaatgtgg gcgtgatttg ttccatctgt
      841 cagcgtagga ttagctgcac cagctcgtgt tggatcaatg acaggcttgt tttcagaagt
//
LOCUS       DACEQH010000185          892 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.194, whole genome shotgun sequence.
ACCESSION   DACEQH010000185 DACEQH010000000
VERSION     DACEQH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.194"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT          315 a          145 c          140 g          292 t
ORIGIN      
        1 atctaaagaa atgttgatcg tcagcaagga gctaaaaaat acgaatatat ttcttttgta
       61 gtaaaatcgg actggtttgc atatcatttt cttcgtaaat atcaaaatca tgttaactac
      121 tatcaattac taataaaaac taccacttta agcgttaaga actgttaagt ttagttaagt
      181 gaggatagtt cacaaaatcc attgttaaac ttcacgaaag tggtaaaact caaaatgctt
      241 tatataggat tacagcatcg ctctttcttc gattgcgcgt tgggtaaaaa atattatgaa
      301 gtaaaaattg acgatgacac gttccttacg gctcatcaga tgaaacagct acaaaaacga
      361 aatgatcaac tcgaagaaaa tttaattcta aaaaagcgat tgccatattc acgcctcgct
      421 cgacgaacta ttaaaggtca agatttacct gaagatcatc ctttttagga aattgaagct
      481 ttttatgttg cacaatgcat ttataatact acaatgatgt tttcaccaga tattcttcta
      541 gggtgcggtg taatgaaaca gatacacttg caaaagaaaa ttcaccgcga atttactaaa
      601 attatgaata attatgttaa tcttccgaaa tttttttcca aaattaaggg atcaagctgg
      661 tgttatagat tgtttaaggt tggcaaaaaa ataacttctt tttaaacaat tatagaaaac
      721 cgatactctg ttaagttggt tgacacggtg tcgattttta tgactttctt attctgaaac
      781 ttcactaacg gacagtacct aaaaagtcta tagtatggac gtttacgagt actattttat
      841 caaaaatgct gctcatttca acttatatgt tttagtgaac tacccccact ta
//
LOCUS       DACEQH010000186          890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.195, whole genome shotgun sequence.
ACCESSION   DACEQH010000186 DACEQH010000000
VERSION     DACEQH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.195"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..888
                     /locus_tag="IW833_002828"
     CDS             <1..888
                     /locus_tag="IW833_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ1308859.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACEQH010000187          887 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.196, whole genome shotgun sequence.
ACCESSION   DACEQH010000187 DACEQH010000000
VERSION     DACEQH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.196"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..798)
                     /locus_tag="IW833_002829"
     CDS             complement(<1..798)
                     /locus_tag="IW833_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005470778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1308860.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKIN"
BASE COUNT          266 a          177 c          139 g          305 t
ORIGIN      
        1 attaatttta gcaaaagcta gtgcatcttg aatcaattcg agcacttctg ccttcgactt
       61 atttttagcc tgtgcgttct tcgctaattg ggcagcagct cgtgctaagt aataagtcaa
      121 atggttatcc gaacaaatat cgattgcgta atttagtagt gcatcacttg tttccaaatc
      181 accgtcttgc gcaaaaaaaa caccacaatg aaagacgata tttaacaccc gccagatatc
      241 ttctgttgta cgaatcggat acgtataaat tttttcaaac actcgattga aataaacttc
      301 tgctttttcc aactcttttt ctctagcata gaccattcca atacctgtat atgccaataa
      361 acgaaacaac gtgttggctg tttctgccat caacaactga tcaaaatgga acaacacatc
      421 cgtaattggt gcattcgtaa aaatcatttc aaatcctttt agatagtagt aacgtgcaat
      481 cagctcagac tcgtcaatta ccttgggatc aatggctgct aaaatctgtg tcaattgttc
      541 atattcactc gtaatcagtg aaaactctgc tttttctagc tgttcatcaa gttgtgtatt
      601 gatcacgttc atcgaagaaa aaagttcaac taacggaaga ttgattcgat tacataattt
      661 gattaaaatt tttaacgaag ggacttgacc attgttctca aatttactta atgttgcttg
      721 tgtacaaatt ccttcagaca cttctttttg agataagttg gcagcctttc ttgccgtaat
      781 aaatttttca atgttcatta ttttctctcc aatcagacct cacacgaaaa tcatttctgt
      841 ctcgatttta gcatgttttc tttgggaatg ctttaattgg ttaagat
//
LOCUS       DACEQH010000188          882 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.198, whole genome shotgun sequence.
ACCESSION   DACEQH010000188 DACEQH010000000
VERSION     DACEQH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.198"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            429..608
                     /locus_tag="IW833_002830"
     CDS             429..608
                     /locus_tag="IW833_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308861.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
BASE COUNT          292 a          135 c          180 g          275 t
ORIGIN      
        1 acaacggatt ccatatcaac gatttgaaag aaaaaaaggt caataatcat ttataccgaa
       61 aagtgagaat gtgtgaaaca aggacaaaaa acgcctagta actaaggaaa tggttcttat
      121 ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa acgggcaaag
      181 cccgataact taagagaaag aaggtgatcc atttgaacaa gattattccc ttattgttag
      241 tactgtttgt tggcgaaata cttatgcgag atagtgcagc tgtacagtta aacctcgctg
      301 tatttttggt aatactggtg ttttgtttga tcttttggcg gtttgtatta gtcctcgtga
      361 gtctactatt caagatgatt gcagccatat ttttcatagg gttatttcga tgaaaggagt
      421 gagtaccaat gccaattgca ataggaaata aacggttacc cgtgacatta gatgaaaaga
      481 gacaaaaaga attgcagcaa ctaaagcaga agtatggcaa aagtgaatct aagattatgt
      541 gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatgta ccagcgctaa
      601 aaaagtgacg tcacttttta atttgatcta tataagtgat atcactttta ccaaggaggg
      661 aattaaatgt taaaaactaa aaagaattcg tacaaattaa agcttagtat actatctatt
      721 atttgtcttt taggcatgat aattagtggt catttgtata gagtggcata tacgcagttt
      781 attgagtaca aggttcactc tttaggatta gctggggatc gatacgttga atatgtccat
      841 tattcattaa aatgttcttc atatattctt ctttggctta tt
//
LOCUS       DACEQH010000189          860 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.199, whole genome shotgun sequence.
ACCESSION   DACEQH010000189 DACEQH010000000
VERSION     DACEQH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.199"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..856
                     /locus_tag="IW833_002831"
     CDS             <1..856
                     /locus_tag="IW833_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1308862.1"
                     /translation="KRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHA
                     LLDIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFH
                     IVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRA
                     SFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPET
                     YTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFKLRIMIR
                     HGKTFLTK"
BASE COUNT          273 a          173 c          165 g          249 t
ORIGIN      
        1 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
       61 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      121 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      181 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      241 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      301 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      361 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      421 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      481 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      541 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      601 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      661 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      721 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      781 gtacggcttt cgcaactttt acaatttcaa actacgaatt atgattcgtc atggcaaaac
      841 atttttaacc aaataaacag
//
LOCUS       DACEQH010000190          823 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.200, whole genome shotgun sequence.
ACCESSION   DACEQH010000190 DACEQH010000000
VERSION     DACEQH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.200"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            22..>823
                     /locus_tag="IW833_002832"
     CDS             22..>823
                     /locus_tag="IW833_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1308863.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT          253 a          142 c          186 g          242 t
ORIGIN      
        1 cgaggagggt ggaaagaaac cttgccagaa attgcggatg aaatcagtat cctcaacttg
       61 acgagtaagt cagaaaatca aggtctgctt gatccttatg tgattatgaa gaaaaagaaa
      121 gactcggaaa gtttagcaat tgatattctg acttttttaa caggaatttc aagtcgagac
      181 ggggagaagt ttcctgtgtt acgtaaagcc attcgaacgg tgactcaaag tgatcaacgg
      241 gggttattac aagttattaa cgaattgcga aaagacgggt caccaatcgc ggaaaatatc
      301 gccgatcata tcgagtcctt taccgattat gattttgctc atttgctttt ctcagacggc
      361 tcgattaccc aatcgattag tttagataaa cagctaaata tcattcaagt ggcggatttg
      421 gttttaccgg atgcagaaac gacttttgaa gaatatacca ccatggaatt gcttagtgtt
      481 tcgatgctaa tcgtcatttc cacttttgcc ttggacttta tccatagtaa ccgagaaatc
      541 ttcaaaattg tcgatttgga tgaggcttgg agttttcttc aagtggctca aggaaaaacg
      601 ttatcgaata agcttgttcg tgctggtcgg gccatgaatg ctggggtcta tttcgtcact
      661 caaaatagcg acgatttatt agatgaacgt ttaaaaaata atatcgggtt gaagtttgct
      721 tttcgcagta ccgatattca tgaaatcaag aaaaccttgg aattctttgg tttggatcag
      781 gaggatcaaa gcaatcaaaa acgcctgcga gatttggaaa acg
//
LOCUS       DACEQH010000191          822 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.201, whole genome shotgun sequence.
ACCESSION   DACEQH010000191 DACEQH010000000
VERSION     DACEQH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.201"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(326..709)
                     /locus_tag="IW833_002833"
     CDS             complement(326..709)
                     /locus_tag="IW833_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1308864.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
BASE COUNT          246 a          144 c          128 g          304 t
ORIGIN      
        1 tactttttgt cttgctaaga tttttaggat caatgataaa aaccgcatca cttatgatac
       61 ggagctgggt atcatgagtg aatgatattt ataaatcact ggatattgaa gtatattctg
      121 ttttctcaac ttatttagtc agatttttta atgaagattt tttgagtaaa atttctataa
      181 ttcctactta ttgaaaagta gtttgtagtg ctgcttctac ctctctcatc tgttctacaa
      241 attccaaata acttgacgca gcttgcggaa tgaaaaaatg atatgtgaat atttctcctc
      301 ctagcccgct taatatgcct attttttata cccatgattt gcaggaaggg gatcggaagt
      361 aggttgcaga aagccgcctt tcataatctg tcggaaaccg gacatcaaaa cggattattt
      421 tacttgtcac atttgtattt tcttgtgagg aagtgccaaa tacattattt acttgagtga
      481 cattctcatt gtcctgtctg atattgcttc gtataaaagt accagatcca cgtctcgtat
      541 ataacaaacc ttcatcaata aggagttgta tagctttgcg gatagtaaca cggcttgttt
      601 taaactctct agccagactt tcttgctcag gtaatttaga accttcctga tattttccct
      661 caacgatcct ctcttttaga gccaaggcta tttctttata ttttggcata ttttcccctc
      721 atttcattga tcctacaaaa aaattagtat atatacaaat ttttgtaaag tatatatact
      781 aataaataca aaaatatttt tattgttgga tacagaaatc ta
//
LOCUS       DACEQH010000192          792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.202, whole genome shotgun sequence.
ACCESSION   DACEQH010000192 DACEQH010000000
VERSION     DACEQH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.202"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..782
                     /locus_tag="IW833_002834"
     CDS             <1..782
                     /locus_tag="IW833_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1308865.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          243 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc tt
//
LOCUS       DACEQH010000193          778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.203, whole genome shotgun sequence.
ACCESSION   DACEQH010000193 DACEQH010000000
VERSION     DACEQH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.203"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            74..754
                     /locus_tag="IW833_002835"
                     /pseudo
     CDS             74..754
                     /locus_tag="IW833_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          268 a          131 c          162 g          217 t
ORIGIN      
        1 ggttctgttg caaagttttc caaaaaatct attttagtgt aaaattgaga aaaaagacag
       61 agaggacaga gtaatgaatc attttaaagg caaacaattc aaaaaagacg tcattattgt
      121 cgctgttggt tactacctgc gttacaatct aagctatcgt gaagttcagg aattgttata
      181 tgatcgtgga ataaatgttt gtcatactac gatttatcgt tgggtataag agtacagcaa
      241 agtcctctat tatctttgga agaagaaaaa tagacaatcc ttctattcat ggaaaatgga
      301 cgaaacctat atcaaaatta agggacgttg gcattatctt tatcgtgcaa ttgatgcgga
      361 cggcttaacc ttagatatct ggttacgaaa gaaacgggat acgcaagcag cctatgcttt
      421 cttaaaacga ctccataaac agtttggtga gccgaaagca attgtgaccg ataaagcacc
      481 ttctcttggc tccgccttta gaaagttaca gagtgtgggt ttatatacta agacagagca
      541 ccgaactgtg aagtatctta acaatttaat agaacaagac catcgaccta ttaaacgacg
      601 aaataaattt tatcaaagtc tccgtacagc ctcttccacg attaagggca tggagaccat
      661 tcgaggaata tataaaaaga accgaagaaa tggaacgctc ttcggctttt cggtgtctac
      721 tgaaatcaag gtattaatgg gaataacagc ctaagatatt tggagttcag agagggtg
//
LOCUS       DACEQH010000194          772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.204, whole genome shotgun sequence.
ACCESSION   DACEQH010000194 DACEQH010000000
VERSION     DACEQH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.204"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..204)
                     /locus_tag="IW833_002836"
     CDS             complement(<1..204)
                     /locus_tag="IW833_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1308866.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMK"
     gene            complement(201..425)
                     /locus_tag="IW833_002837"
     CDS             complement(201..425)
                     /locus_tag="IW833_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1308867.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(422..715)
                     /locus_tag="IW833_002838"
     CDS             complement(422..715)
                     /locus_tag="IW833_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308868.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
BASE COUNT          223 a          158 c          122 g          269 t
ORIGIN      
        1 tttcatgttg gcggctacgt ttcttgctgg tgcatctatt ccagtttcgt gcaaaataat
       61 tttgttagga attgctaatc tggagtctcc ttgattcggc gctaaattat actcgtcatt
      121 aatagtataa gcaaacgtat taatgggtaa taaaaaaaga gccgttaaca ggctcatcgc
      181 agtaatagta attttctttt tcatttgttt cctccttctt cgctttcagc cgagaacatt
      241 ttgtaggttc gatttgatac acccaacaca ctccctaaaa acgcgccaaa accagtaatg
      301 atgacaacac agatatctgt gtactgccaa ttgagcgctt taccaactaa ccccacgaaa
      361 gtagctagtg cgggaataat taccagtgcg aaccatttta gtacttcgaa cgttttatta
      421 ttcattttct tctctcccta aataaagttt taatttgttg cgtgtgttct accaattttt
      481 ctgcatgtgt atctaatctt tcatcgtgtt tctttagttc ttcatgaatc atcaatcgat
      541 ctgatttgct cgattctaaa tctttagtca gcaaatctaa attgtgactt acttttgaaa
      601 gagtctcagt aatcttcgag aaagatgcag taattggttt tattactaat aaaatcaaag
      661 aaacgatagc ggttattgat cctgctatcg ctccccattc ccctaaatta atcatgtgac
      721 aactccttga atcaaaataa aaagcacatc aattaagatg cgctctcttc tt
//
LOCUS       DACEQH010000195          743 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.205, whole genome shotgun sequence.
ACCESSION   DACEQH010000195 DACEQH010000000
VERSION     DACEQH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.205"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..653
                     /gene="xerS"
                     /locus_tag="IW833_002839"
     CDS             <1..653
                     /gene="xerS"
                     /locus_tag="IW833_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ1308869.1"
                     /translation="VATITDKTTLAARANAIEKKLFLDEDTQNYLDYIANEYKKTLSK
                     RALIYYQRDVERDLAINALILGSGLRLSEVVNINLEDLSLEKNNVVVTRKGNKRDAVN
                     IAAFAMEYLANYLAIRKERYKVTENEKALFLAIYQGEAKRISGIAIERMVAKYSKGFR
                     VQVSPHKLRHTLATRLYQQTNSLVLTAQQLGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          295 a          119 c          127 g          202 t
ORIGIN      
        1 aagtagcaac tatcacagat aaaactacgt tagctgcccg tgcaaacgca atcgagaaaa
       61 aattatttct agacgaagat acacaaaatt atctagatta tattgccaat gaatataaaa
      121 aaactttgag caaacgagct ttgatctatt atcaacgaga tgtagaaaga gacctggcga
      181 tcaatgcatt gattttagga tctggattaa gactctccga ggttgtaaat attaatttag
      241 aggacctatc tttagaaaag aataatgtgg tagtaactag aaaaggaaat aagagagacg
      301 cagtaaatat agcagcattt gcaatggaat atttagctaa ttatctagct attcggaaag
      361 aacgatacaa agtaacagaa aacgaaaaag ctttatttct agcaatttat caaggagaag
      421 ccaagcgtat atccggtatt gcaatcgaac ggatggtagc aaaatattca aaaggattca
      481 gagtccaagt cagtccacat aaattacgtc atactttggc tactagactc tatcaacaga
      541 caaattcatt ggtattaact gcccaacaat tgggacatac aagtacaaat acaacaactt
      601 tatatactca tatagacaat gcagctacaa ttgatgcttt gaacgctttg taaatgtagt
      661 aaaaaatagt ataataatga agcctgtctc taaaattaga taaaagccca actttaaagg
      721 caagcttcat tatcattttt ata
//
LOCUS       DACEQH010000196          699 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.206, whole genome shotgun sequence.
ACCESSION   DACEQH010000196 DACEQH010000000
VERSION     DACEQH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.206"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(128..>699)
                     /locus_tag="IW833_002840"
     CDS             complement(128..>699)
                     /locus_tag="IW833_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAQ1308870.1"
                     /translation="KTANVLDRTFTQEQPLEAIVTDLTYVRVGKKWHYICLILDLFNR
                     EIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKEFDNQTIHEILNGFGITRS
                     LSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAY
                     ETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          198 a          159 c          130 g          212 t
ORIGIN      
        1 gaatggcaac actttttttg acaaaaatta taaggtgttg tgaatgctgc ggtgttatgg
       61 ctacgattgc ccttgacaat gagcatcctc ggatctgatt ttctccggca gaagcacaat
      121 cattcaatta cgctgcctct ccagaggtat cagatccgcg ctcattatca aggattcgct
      181 tcgccaatct ctgttgtcga atttggatcg gtgtttcata cgccaacgtg ccatgtaagc
      241 gtagatagtt ccaccaatgc acataatcaa acaattctag acgtagctgt gccagtgtct
      301 caaattggta ttgatgcacg aattctactt tgacagattt ataggttgat tccacaacgg
      361 cattatcata cggacaaccc ttcctactca atgaacgagt aattccaaaa ccattcagaa
      421 tctcatgaat ggtttggtta tcaaattcct ttccccggtc tgtatgaaaa agcttgacgt
      481 ctgttaaaga atacggtatc cgtccaaagg cttcttttac caatgaggca tctttcttct
      541 caccacagga ataaccaata atttctcgat taaacaaatc aagtattaag cagatataat
      601 gccacttttt ccccacgcga acataagtaa gatccgtaac gatggcttcc aatggctgtt
      661 cttgtgtaaa ggtccgatct aatacattcg ctgttttcg
//
LOCUS       DACEQH010000197          666 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.208, whole genome shotgun sequence.
ACCESSION   DACEQH010000197 DACEQH010000000
VERSION     DACEQH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.208"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..111
                     /locus_tag="IW833_002841"
     CDS             <1..111
                     /locus_tag="IW833_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1308871.1"
                     /translation="ISREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            124..510
                     /locus_tag="IW833_002842"
     CDS             124..510
                     /locus_tag="IW833_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308872.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
BASE COUNT          233 a           78 c          162 g          193 t
ORIGIN      
        1 attagccgtg aagtgggtta ttcagaaaga agcatacaaa ggatgaagtc ggaagctcta
       61 atagagtttg cagaagcgta taaacacgga agaataattg cttataaata gggggaccat
      121 ataatgtgga aagattatgt atcgttgaaa gagttgaaaa aagatcttgt tttcaaaaga
      181 attgtagaat ggtcagagag tgaattaatt ttagaagatg gaaccaaaat ggaagttgta
      241 tgtagcgaat cagattgctg cgcatgggcc gaaggtgaat tcaaaaatgt aaagcttgat
      301 gcagtgatta cagatataaa gatttttgat aaaggtaacc gtctctataa tggtgacgga
      361 catacctctt atgctgaagt cgttgtttat cataatcgta atgagatcgc taaagcagaa
      421 tgcacagcga atgatgggaa tggcggctac tattatagtg tttgtgcttt aaaggtcaaa
      481 gacaaacttt gtatagtgac tgacgcataa aaaaatggcg gttttttggc ggaatgatgg
      541 cggtttttag ccatttatca gtgatattat ggtagtgtcg aaagattagt gataggtctg
      601 agacaaaata ataataaaag gaacatcgtt ttattattgt ttcacaatta agcttcgata
      661 gacagc
//
LOCUS       DACEQH010000198          666 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.209, whole genome shotgun sequence.
ACCESSION   DACEQH010000198 DACEQH010000000
VERSION     DACEQH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.209"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            55..666
                     /locus_tag="IW833_002843"
                     /pseudo
     CDS             55..666
                     /locus_tag="IW833_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          274 a          112 c          125 g          155 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      481 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
      541 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
      601 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
      661 tattga
//
LOCUS       DACEQH010000199          666 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.210, whole genome shotgun sequence.
ACCESSION   DACEQH010000199 DACEQH010000000
VERSION     DACEQH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.210"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(19..>666)
                     /locus_tag="IW833_002844"
     CDS             complement(19..>666)
                     /locus_tag="IW833_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAQ1308873.1"
                     /translation="DYIQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGE
                     IGKYNSLAGSTRLVQKAAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASH
                     ADMLISGIGTLSSIDSVEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTF
                     GLSLKEIFDIPKRIGICKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
BASE COUNT          222 a          122 c          113 g          209 t
ORIGIN      
        1 tggaggattt tacatgactc actctttttt tatcaaagaa agtattttcc aagcagtttt
       61 ttcatccaaa aataaatctg taaaaaaatt accattcaag gcgcctaaac aactctccgc
      121 tttgaactta cttttacaaa taccaattcg ttttggaata tcgaaaattt cttttaaaga
      181 aagaccaaaa gtcttatcct cagatgcttt gatgaattca ccattgatat tgaagggtct
      241 tccgtaaaga aatccaacag tatccgtcat gcttggaaat aggattttac gatgcttgtt
      301 ccagggttcg acagaatcaa tagaggataa ggtgccaatt ccagaaatta acatatctgc
      361 atgcgaagcg gttgctaagg tattagctaa agaaggttct agagccatta gttcacgtgc
      421 agtttcattg atgacatata aaggaatact gagagtcaaa taagaagact cataacgttc
      481 agcagctttt tgaactaatc gtgtggagcc agctaaggaa ttatattttc cgatttcgcc
      541 aatgaattga gtaaaaatta attcttcacg agcacccatt ttaaactgat caatcacttt
      601 gtaaacggta tctccccaag atagtgcaac cacattggct ttcttaatta attcttgaat
      661 gtaatc
//
LOCUS       DACEQH010000200          656 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.211, whole genome shotgun sequence.
ACCESSION   DACEQH010000200 DACEQH010000000
VERSION     DACEQH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.211"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            93..422
                     /locus_tag="IW833_002845"
     CDS             93..422
                     /locus_tag="IW833_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1308874.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            412..>656
                     /locus_tag="IW833_002846"
     CDS             412..>656
                     /locus_tag="IW833_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1308875.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCG"
BASE COUNT          251 a           73 c          126 g          206 t
ORIGIN      
        1 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
       61 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
      121 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
      181 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
      241 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
      301 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
      361 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
      421 gaaatcgttc cttcaaaaca tgtcattaag tatttaaaaa agctaaaaga gaagccattg
      481 aaagaaaaat ttttgaattt aatttatgat gaaattgcga ttgatccata taagggtgaa
      541 caaaaaactg gggatttatc aggtatttgg tctagtgggt tcaaatatgc gggcacaaca
      601 tatagagtag cttacgagat tatagaaaac aaggttattc cagtattgtt atgtgg
//
LOCUS       DACEQH010000201          656 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.212, whole genome shotgun sequence.
ACCESSION   DACEQH010000201 DACEQH010000000
VERSION     DACEQH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.212"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IW833_002847"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IW833_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ1308876.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DACEQH010000202          635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.213, whole genome shotgun sequence.
ACCESSION   DACEQH010000202 DACEQH010000000
VERSION     DACEQH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.213"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..278
                     /locus_tag="IW833_002848"
     CDS             <1..278
                     /locus_tag="IW833_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308877.1"
                     /translation="YASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEAEEFVYPF
                     RGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            355..>635
                     /locus_tag="IW833_002849"
     CDS             355..>635
                     /locus_tag="IW833_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1308878.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVP"
BASE COUNT          208 a          114 c          152 g          161 t
ORIGIN      
        1 tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaacccta
       61 aaacacgcaa gaaatcaaaa ttcaaagtcg gtgacatggt ccgctgtgaa gcagaagagt
      121 tcgtttatcc gttcagagga tacgttaaaa agatactgtc aaactcagca atcattcgta
      181 ttgaaaatac aatgaagtgt gatcgttcga ctgcaaaaca caagcattat ttagctgtag
      241 cgagattagt tgacatggaa gtaatcaaga gcaaataaaa aaagccggat cgctccgact
      301 gattcaataa atccaacaca tttattatat cacataaagg agcggtttga cttgatgcaa
      361 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
      421 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattata
      481 acggatatgc cgaaggcacc gaagcatggc aataaggcag aagacgcgat tattcagatg
      541 atggatatag aaacggagag agatgcgatt ctagcggctt tgatggctct tagtctgatt
      601 agtcgtcaga tactctacta cagcttctgt gtgcc
//
LOCUS       DACEQH010000203          593 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.214, whole genome shotgun sequence.
ACCESSION   DACEQH010000203 DACEQH010000000
VERSION     DACEQH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.214"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..580
                     /locus_tag="IW833_002850"
     CDS             <1..580
                     /locus_tag="IW833_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1308879.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          105 c          122 g          162 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cct
//
LOCUS       DACEQH010000204          567 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.215, whole genome shotgun sequence.
ACCESSION   DACEQH010000204 DACEQH010000000
VERSION     DACEQH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.215"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            79..>567
                     /locus_tag="IW833_002851"
     CDS             79..>567
                     /locus_tag="IW833_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ1308880.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMD"
BASE COUNT          214 a          104 c           92 g          157 t
ORIGIN      
        1 aaaatccata caaaaaagga agtcccttct gtagaataaa gttaacgaca accaattcac
       61 agaaaagagg acttccctat gaatgatttt actacagaaa ttgtgcaaac tctagtcact
      121 aaaggcgatt taaatgaatt attccgttcg cacttagaaa aagcgataaa cacactccta
      181 cggactgaat taacggcttt tttagattac gaaaaatatg atcgcactgg ttttaattca
      241 ggtaattcga gaaacggttc ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca
      301 ttggaaatac ctagagatcg taatggtgag tttaaacaac aaactttacc agcctacaaa
      361 agaacaaacg atacattgga aaccactatt atccatttat tcgaaaaagg tgttacgatg
      421 tctgaaattg ctgatttgat cgaaaaaatg tacggtcatc actatactcc acaaaccatg
      481 tccaacatga ctaaagttct gactgaagaa gtaaatgcct ttaaatccag agccttaaat
      541 gataagtatg tcgctatttt tatggac
//
LOCUS       DACEQH010000205          540 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.216, whole genome shotgun sequence.
ACCESSION   DACEQH010000205 DACEQH010000000
VERSION     DACEQH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.216"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            131..>540
                     /locus_tag="IW833_002852"
     CDS             131..>540
                     /locus_tag="IW833_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1308881.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHFVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEK"
BASE COUNT          200 a          106 c           95 g          139 t
ORIGIN      
        1 ggttcataca aaagcctata accgcatttt aagcatacgc caattctaaa attaagctag
       61 acaactaaaa aagtcatttg tgctacactc aaaatagttc cgtcgaaaga attataagga
      121 gtgagtacaa atgacctata cccatcttac accaactgaa ctcgtgatga tagaagctta
      181 tttcaatcaa tcacactttg tttcaaaagt agcccatcta gtacaacgtt ctcgacaaac
      241 catttataat gtctatcgtt ttcttaaatc tggtggaagc gcaataagct actatgaaca
      301 atataagaaa aacaaacgta actgcggaag acgtccgatt gtgttaccag atgggcaaca
      361 agaatacatt caaaaaaagg tggctcaagg gtggactcca gatgtcctta ttggtcgagc
      421 agagtttcct attgcttgtt cggtacgtac actatatcgg atgtttaaag ataaaacctt
      481 tgaccctcac gatttaccaa tgaaagggaa aagaaaacca aacggacata aagaaaaaag
//
LOCUS       DACEQH010000206          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.217, whole genome shotgun sequence.
ACCESSION   DACEQH010000206 DACEQH010000000
VERSION     DACEQH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.217"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(5..90)
                     /locus_tag="IW833_002853"
     tRNA            complement(5..90)
                     /locus_tag="IW833_002853"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IW833_002854"
     tRNA            complement(107..178)
                     /locus_tag="IW833_002854"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IW833_002855"
     tRNA            complement(190..262)
                     /locus_tag="IW833_002855"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IW833_002856"
     tRNA            complement(280..361)
                     /locus_tag="IW833_002856"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IW833_002857"
     tRNA            complement(369..441)
                     /locus_tag="IW833_002857"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IW833_002858"
     tRNA            complement(445..517)
                     /locus_tag="IW833_002858"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACEQH010000207          519 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.218, whole genome shotgun sequence.
ACCESSION   DACEQH010000207 DACEQH010000000
VERSION     DACEQH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.218"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            158..>519
                     /gene="tnpA"
                     /locus_tag="IW833_002859"
     CDS             158..>519
                     /gene="tnpA"
                     /locus_tag="IW833_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1308882.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTE"
BASE COUNT          179 a           74 c           92 g          174 t
ORIGIN      
        1 cccgtgattt taatcatggg atgaatcgga ttcagttgtg acctagcttt agctaggtta
       61 aacaactgac aaatataaac taaataatag tgaaatgtct tgcaataatt gattttgtag
      121 aaatacatat attaaaataa ttgcgaggtg tttttgtatg tcagaaattc attatggacg
      181 gggttatgtc tattctattc aatatcatct tgtttggtgt gtaaaatatc gacatgatgt
      241 tttgtatggt caggtagata ttgatgtcaa acagtttttg aaacaaattg ctgatgataa
      301 taatataaaa attatagaaa tagaatcaga taaagaccat attcatttac tcattgaatg
      361 caccccacaa cactatattc ctagtattgt taaagtattt aaaggcgcat ccgctagact
      421 gctgtttaaa aaacatcctg aacttaaaca actcctttgg ggtggacatc tttggaatcc
      481 aagctacttt gtagctactg ttagcgaaaa tacagaaga
//
LOCUS       DACEQH010000208          513 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.219, whole genome shotgun sequence.
ACCESSION   DACEQH010000208 DACEQH010000000
VERSION     DACEQH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.219"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            188..513
                     /locus_tag="IW833_002860"
                     /pseudo
     CDS             188..513
                     /locus_tag="IW833_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          189 a          103 c           98 g          123 t
ORIGIN      
        1 ggctaccttt ttaggtagcc tactttaatc tttatacctt tctggatcaa cgaaagtata
       61 ctttatatag tcataacgcc gatgatcgct acgtgcgtcc ggcacgtcag tcatgaatcg
      121 gctacactag actagacaga aaaaataagg tgtgtagaat aataaaaaac acactggagg
      181 atttttcatg tcaagacgtc aacgaagaac ctattcaaaa gaattcaaac aacaaatcgt
      241 cgatctctat ctcgctggta agcctcgcgc agaaattatt cgagagtatg agcttacgcc
      301 ttcttctttc gataaatgga tgaagcaagc acaatcaacg ggctcattca aagaaagaga
      361 caacttaaca ccagaacaag cagaattgat cgcactaaga aagaaaaata agcaactcga
      421 aatggagaat gatattttaa agcaagcggc gctgatattc ggacgaaaag acaagtaatt
      481 gatgccaaca agcataaata ttccatatca gcg
//
LOCUS       DACEQH010000209          510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.220, whole genome shotgun sequence.
ACCESSION   DACEQH010000209 DACEQH010000000
VERSION     DACEQH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.220"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..508
                     /locus_tag="IW833_002861"
     CDS             <1..508
                     /locus_tag="IW833_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HAQ1308883.1"
                     /translation="DLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFS
                     RIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLK
                     VEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELG
                     CANASEAV"
BASE COUNT          155 a           90 c          110 g          155 t
ORIGIN      
        1 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
       61 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      121 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      181 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      241 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      301 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      361 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      421 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      481 atgtgctaac gctagcgagg cagtctaact
//
LOCUS       DACEQH010000210          507 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.221, whole genome shotgun sequence.
ACCESSION   DACEQH010000210 DACEQH010000000
VERSION     DACEQH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.221"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(205..>507)
                     /locus_tag="IW833_002862"
     CDS             complement(205..>507)
                     /locus_tag="IW833_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1308884.1"
                     /translation="LGKKGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGV
                     MKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          145 a          115 c           88 g          159 t
ORIGIN      
        1 ttttttgtaa aaaactattg cacaatacaa gtggcctacg gctatcgaaa tttccataac
       61 tttcgagcaa gaatttatct cattcaaggc ttgatcttcc aagtaaaaca aaaaccagtt
      121 aagcactctg cctaactggt tatgactaaa ttttcagttg tatctttctc taccaacact
      181 aaatgtcgaa gagccttttt tcgtttatga atgtaagacc gcttctcgag ccaatgccaa
      241 acaaccgagc gttccagcgt tgtcttctaa tgctggggtc acgatatatt tgcttaactc
      301 aggtgtttcg acatacccat tgaccaattt tgcaaatgcg tcgtggacgt tttgcagcat
      361 atggcgttgt ttcatcacac caccgccaaa aatgatcaca tctggagaaa acatcaaggt
      421 tgtattgtag gcacattgag caatatagga ggcttcgatt tcccaataag gatcatcttc
      481 tgcaacctct tgtccttttt tccctaa
//
LOCUS       DACEQH010000211          488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.222, whole genome shotgun sequence.
ACCESSION   DACEQH010000211 DACEQH010000000
VERSION     DACEQH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.222"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..414)
                     /locus_tag="IW833_002863"
     CDS             complement(<1..414)
                     /locus_tag="IW833_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308885.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFF"
BASE COUNT          185 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaaacggc tttccaaatg atgaaatacc taaatctaac ttattaagaa agttgaaatt
       61 tttatattca ggaaaccatt tattaatttc atgtgcagca cgtaatgcat tccctggagt
      121 tgtcaaagag aaaattaatt ctgctaagct tattattgaa aatactgata acttataatt
      181 atatcgtcca ttgacaatca aatataaact aactatactt gttagaacaa aaaaacaggc
      241 gttgacttgt tcttgattag ttaaaaatat caataaagga atacttaccc aatatatttt
      301 tctatttatt tcttctcctc ttaagagttg aaaaaacgga taaaaagtta gtaaactaaa
      361 tgctactggc cactgatagt tcaaggtggt tgctctccaa ccggtttccc ccattactgt
      421 caatggtatt aaacagtata tagagcaaac caataataaa tttttaacat ttattttctc
      481 aaaaacat
//
LOCUS       DACEQH010000212          488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.223, whole genome shotgun sequence.
ACCESSION   DACEQH010000212 DACEQH010000000
VERSION     DACEQH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.223"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            225..>488
                     /locus_tag="IW833_002864"
     CDS             225..>488
                     /locus_tag="IW833_002864"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1308886.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLS"
BASE COUNT          157 a           97 c           75 g          159 t
ORIGIN      
        1 atattttcaa aaaccttttc ataatcctca ccctttctta cactgataaa tcatgcgtct
       61 aatattcaaa ttatactcga tatgctttag ctaatcagcc aactctgttt gtctactatc
      121 attatatcaa tagacaatat ctgctcccgt ctttatgatc gtgccaaagc aatcaaaaga
      181 aaattgcgaa tcacttagat cgaaagttta ctcaaacaaa agcattgtca gcggttgtca
      241 cggatttaac ttatgttcgg gtcgggacaa attgggctta tatttgtttg atgattgatt
      301 tattcaatcg tgaaattatt ggccattctt gcggaaataa aaaagatgcg cagcttgtaa
      361 aaagggccat tcaaagcatt ccttactcac ttcaagaaat cgaactcttc catacggatc
      421 gtggaaaaga atttgataac caaaccattc agaatctcat gaatggtttg gttatcaaat
      481 tcctttcc
//
LOCUS       DACEQH010000213          462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.224, whole genome shotgun sequence.
ACCESSION   DACEQH010000213 DACEQH010000000
VERSION     DACEQH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.224"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..430)
                     /locus_tag="IW833_002865"
     CDS             complement(<1..430)
                     /locus_tag="IW833_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1308887.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DACEQH010000214          438 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.226, whole genome shotgun sequence.
ACCESSION   DACEQH010000214 DACEQH010000000
VERSION     DACEQH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.226"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            71..>438
                     /locus_tag="IW833_002866"
     CDS             71..>438
                     /locus_tag="IW833_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ1308888.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQT"
BASE COUNT          177 a           56 c           60 g          145 t
ORIGIN      
        1 ttagaactta cttaaatcct attataatat ttttgttaat gattttaata aaatgattgg
       61 agagaaaatg atggatcaaa aagcgtttat cgaatctatt gattctacaa tcgatagatt
      121 aaaaaaagaa attcgatttt ataatagagt aattggtatc ggaaatatta taaaaatagt
      181 cttatcagct agtatcccaa tattaattga tcaggcttcc gaacacaggt ctttactatt
      241 gattgtttca attgcttcag caataattac aattatacaa agtgggatgt ctgcatttaa
      301 ttatcaagat aaagtacaga cttcgacaga gttattaatg aaaattgaaa aagaaaagtt
      361 gttatatata acgaaaacat ccccatataa taaaacagat gaagaaaatt tccatttaat
      421 cgttacaacc cttcaaac
//
LOCUS       DACEQH010000215          432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.227, whole genome shotgun sequence.
ACCESSION   DACEQH010000215 DACEQH010000000
VERSION     DACEQH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.227"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..417
                     /locus_tag="IW833_002867"
     CDS             <1..417
                     /locus_tag="IW833_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1308889.1"
                     /translation="TSDDKPVIDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVDG
                     WHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPN
                     KKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          156 a           75 c           86 g          115 t
ORIGIN      
        1 acttcagatg ataaaccagt cattgatcca actagagcag gtgcagcaaa tcctacgctg
       61 acagatggaa caaatcacgc ccacattgat cagtttggag aaatcgaaaa cgcaaacttg
      121 catgtggatg gatggcacat tgctaattat aaatacgagt atattttcat tatggattac
      181 aatactggaa aagaattagc tcgagtaaga gctgatggaa tttatagacc agatgtaaat
      241 caagcttata atacttctgg aaacgttggt tatcatgtat ctttcaatat gcgtaatttt
      301 cctaataaga aagtctatgt aatgatgcgt gcaacgaatg atccagaagg gaacactaaa
      361 ggcggagcac aagattttca tgataaacgc tggtatttaa atattccgca acgataaaaa
      421 aagcccctcg at
//
LOCUS       DACEQH010000216          430 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.228, whole genome shotgun sequence.
ACCESSION   DACEQH010000216 DACEQH010000000
VERSION     DACEQH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.228"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>430
                     /locus_tag="IW833_002868"
     CDS             <1..>430
                     /locus_tag="IW833_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAQ1308890.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          143 a           84 c           73 g          130 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc
//
LOCUS       DACEQH010000217          390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.229, whole genome shotgun sequence.
ACCESSION   DACEQH010000217 DACEQH010000000
VERSION     DACEQH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.229"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT          178 a           50 c           60 g          102 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc
//
LOCUS       DACEQH010000218          366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.230, whole genome shotgun sequence.
ACCESSION   DACEQH010000218 DACEQH010000000
VERSION     DACEQH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.230"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..364
                     /locus_tag="IW833_002869"
     CDS             <1..364
                     /locus_tag="IW833_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HAQ1308891.1"
                     /translation="LANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHN
                     YQAMHDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTS
                     ASTHDETAVDAVSSASVK"
BASE COUNT          135 a           64 c           80 g           87 t
ORIGIN      
        1 attagccaac tgtcacccag aaaaatcgta taaaaatgta gagtgggtaa ttcaacaact
       61 gcgaagcgga gcaacagatg cggtacgtgt tcatgtacca aaacaagtgc cagaaaaata
      121 tattgtgcat aattaccaag caatgcatga tgaaaatgga gagtatgctg gaatcaatga
      181 atacattctt gatttcaaac caatcattga gtggtattta caacaaacgg gtcaacaact
      241 tgttggaaat gaagatgcgg tttcaagtgc atctgttcat ggtggaaaaa cagatgcagt
      301 aaccagcgcc agcacacacg atgaaactgc agtagatgca gtttcgagtg cttctgtaaa
      361 ataaaa
//
LOCUS       DACEQH010000219          366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.231, whole genome shotgun sequence.
ACCESSION   DACEQH010000219 DACEQH010000000
VERSION     DACEQH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.231"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            80..>366
                     /locus_tag="IW833_002870"
     CDS             80..>366
                     /locus_tag="IW833_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1308892.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCR"
BASE COUNT          129 a           59 c           76 g          102 t
ORIGIN      
        1 tttaggtatt aaaaaacata tcgttctcta gtatgattaa atcacgacaa aataaacaac
       61 tgagaggacg atatgctata tgaatgattc tatcaaaaaa atgctgcgat taatagagaa
      121 agatttgatg attacagagg tctcttacga gacctttcag aagaaaaaga cgttgatcgt
      181 cgatgctgtt ttctcacctg ctccccatac ctgtagaaat tgtggttcta ctgtggtaga
      241 tggaaacggg aaagtaattg tagtgaaaaa tgggaagaag gaaacgattg tccgctttga
      301 acaatacaat cacatgcctt tggttatgcg cttaaaaaag cagcgctata cctgtaaaaa
      361 ctgtcg
//
LOCUS       DACEQH010000220          362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.232, whole genome shotgun sequence.
ACCESSION   DACEQH010000220 DACEQH010000000
VERSION     DACEQH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.232"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>362)
                     /locus_tag="IW833_002871"
     CDS             complement(<1..>362)
                     /locus_tag="IW833_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1308893.1"
                     /translation="HRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTE
                     GFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIA
                     FGYRNFYNFRARIYLQQG"
BASE COUNT          107 a           66 c           75 g          114 t
ORIGIN      
        1 gaccttgttg taaatagatc cgtgcccgaa agttataaaa gttgcgataa ccaaacgcaa
       61 ttcgtttaat cagcttgatc ttgttattca gcccctcagt gacgccgttt gagtgggaca
      121 ccttaaaggc atttgaaata cccgattggt agcgatggaa gatcgtgaat ttctttttaa
      181 acgactgagg taacgtagat gaaattgttt taagggtttc ttgaaagcct tccgtacctc
      241 gtttaacgaa ataatagcgt aataattgac aaaaatgata cgcagttcgc aattcttcgt
      301 tgtagcttaa cagctcatca acgatttctg tttgactaat tggtcgttta aacagtcgat
      361 ga
//
LOCUS       DACEQH010000221          338 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.234, whole genome shotgun sequence.
ACCESSION   DACEQH010000221 DACEQH010000000
VERSION     DACEQH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.234"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT          102 a           62 c           73 g          101 t
ORIGIN      
        1 tccggtcgta ggttcgagtc ctacaaggta cataagaggt tgtgaaaaag ctgccctgca
       61 tcaagtaata agaacagcca agtaaaaaca gcttcttatg tttttacttg gctgttctta
      121 ttactgcaga tgcaagcttt tgaataccgt ttaaggaaga ggttgtgaaa tcagcgtttt
      181 gacctgagta ttagcttaac aaacggaaaa atagttcctc atattttgcc gtttgttaag
      241 ctaataccga gggtcagctg attgaacacc atttaagaaa gaggtcgtga gcctgccgtt
      301 tcactctgtg tcataaaaaa gccaatctac tttgatag
//
LOCUS       DACEQH010000222          333 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.235, whole genome shotgun sequence.
ACCESSION   DACEQH010000222 DACEQH010000000
VERSION     DACEQH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.235"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>333
                     /locus_tag="IW833_002872"
     CDS             <1..>333
                     /locus_tag="IW833_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308894.1"
                     /translation="QPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAF
                     EGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDA
                     DKYEPTVEG"
BASE COUNT          137 a           48 c           74 g           74 t
ORIGIN      
        1 tcaaccaatt acagcagatg atacagaatt tgtatcaagt gcaacaaaac tggatgataa
       61 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
      121 accagacgca tttgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      181 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      241 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      301 tgacgccgat aaatatgagc caacagtaga agg
//
LOCUS       DACEQH010000223          322 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.237, whole genome shotgun sequence.
ACCESSION   DACEQH010000223 DACEQH010000000
VERSION     DACEQH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.237"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>322
                     /locus_tag="IW833_002873"
     CDS             <1..>322
                     /locus_tag="IW833_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1308895.1"
                     /translation="DGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKALFEKNY
                     RAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVS
                     KEKLA"
BASE COUNT          103 a           48 c           76 g           95 t
ORIGIN      
        1 gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg ggagctggta
       61 acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat aaagcattgt
      121 ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa aaatatggaa
      181 ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt tctcatttgt
      241 gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta tcgcaaatgg
      301 gagttagcaa agaaaagctt gc
//
LOCUS       DACEQH010000224          316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.239, whole genome shotgun sequence.
ACCESSION   DACEQH010000224 DACEQH010000000
VERSION     DACEQH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.239"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>316
                     /locus_tag="IW833_002874"
     CDS             <1..>316
                     /locus_tag="IW833_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1308896.1"
                     /translation="KQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFI
                     GTMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEG
                     FSH"
BASE COUNT           80 a           66 c           83 g           87 t
ORIGIN      
        1 aaacaatacc aagcagattt agcagggatt gggattggct catttgggcc tatcgatatc
       61 catcgcgatt ccgccactta tgggtacatt acctcgaccc cgaaattggc atggcaaaat
      121 tttgatttca tcggtaccat gaaaaaagaa tttccgatcc cgatttcttg gacaacagat
      181 gtcaatgcgg ctgcctatgg tgagtatgtc ttcggcagtg gcaaaggatt gagcagtgtc
      241 gtttactata cgatcggtac gggtgttggc ggcggtgcct tacaagaagg gcgctttatc
      301 gaagggttta gtcatc
//
LOCUS       DACEQH010000225          316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.240, whole genome shotgun sequence.
ACCESSION   DACEQH010000225 DACEQH010000000
VERSION     DACEQH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.240"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            35..>316
                     /locus_tag="IW833_002875"
     CDS             35..>316
                     /locus_tag="IW833_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1308897.1"
                     /translation="MNTYQPESVEDMQDVLKDVFGPLFEKMLQGELNNHLGYDAHSKE
                     PKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIPNRKRDVSDI"
BASE COUNT          108 a           55 c           67 g           86 t
ORIGIN      
        1 aaatcctgat gctgagaagt tagctgaatc cattttgaat acttatcaac ccgaatctgt
       61 agaggatatg caagatgttt taaaagatgt gtttggtccc ttatttgaaa aaatgcttca
      121 aggagaattg aataaccatt tagggtatga cgcccattct aaagagccaa agaaacatga
      181 caatcgacga aacggttatg gcaataagac gcttaaaacg agctttggta aagtaaacat
      241 tgatgttccc agagaccggg aggcttcttt tgaacctgag ttgattccta atagaaaacg
      301 agatgtctcc gacatc
//
LOCUS       DACEQH010000226          309 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.241, whole genome shotgun sequence.
ACCESSION   DACEQH010000226 DACEQH010000000
VERSION     DACEQH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.241"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            51..>309
                     /locus_tag="IW833_002876"
     CDS             51..>309
                     /locus_tag="IW833_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1308898.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKW"
BASE COUNT          120 a           41 c           61 g           87 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggc
//
LOCUS       DACEQH010000227          304 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.242, whole genome shotgun sequence.
ACCESSION   DACEQH010000227 DACEQH010000000
VERSION     DACEQH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.242"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            83..271
                     /locus_tag="IW833_002877"
     CDS             83..271
                     /locus_tag="IW833_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308899.1"
                     /translation="MLKKTMLVLGSISLGVLGFSPMLLSVIGKNNSKGELKLNPNYYN
                     SPNEPMFISEKQNDGQDK"
BASE COUNT          113 a           53 c           48 g           90 t
ORIGIN      
        1 aatttaaaca tttgacaaac tatagaacag cacttaaaat gtgtatacaa gtgacagcgc
       61 tttaaaaaaa gaggtgcagc ctatgttaaa aaaaactatg ttggtactag gttctatttc
      121 tctaggagta ttaggtttta gccccatgct attatccgta attggaaaaa ataattccaa
      181 aggggaactt aaattaaatc caaattacta caatagtcct aatgaaccta tgtttatttc
      241 agaaaaacaa aacgacggtc aggacaaata atcctgccgt cgttttgctc taacttctac
      301 cagt
//
LOCUS       DACEQH010000228          296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.244, whole genome shotgun sequence.
ACCESSION   DACEQH010000228 DACEQH010000000
VERSION     DACEQH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.244"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>296)
                     /locus_tag="IW833_002878"
     CDS             complement(<1..>296)
                     /locus_tag="IW833_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1308900.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEK"
BASE COUNT           80 a           60 c           50 g          106 t
ORIGIN      
        1 tccttttcta agtccaaagt aaaagggcgt tttccagcct ctattcgttg acgattgatc
       61 tcatcttcta attcatcctg ataggctttc gcttcatcat gggtcatttc tttcgtaaac
      121 ttatgtttat tggcgttagc tttgatgtgg gtagaatcca gatacaaatt atcctctgtt
      181 acaaaacctg ctttaactgc ttgttctaaa atataggcaa aaatatcctc aaacaccgtc
      241 gtttctctaa atcggcgaac ataattcttt ccaaaagttg agaaatgggg gatttt
//
LOCUS       DACEQH010000229          292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.245, whole genome shotgun sequence.
ACCESSION   DACEQH010000229 DACEQH010000000
VERSION     DACEQH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.245"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT           91 a           53 c           47 g          101 t
ORIGIN      
        1 gttcagtcca agccaactaa aaagttttcg ggatacattc tccttctctt cctaataatc
       61 aattaaaatt ttaatcttta tatttctgct aatcactcag gaaacagtac agaaaaacaa
      121 acacgtatca tagatataaa tgtaatggca tagtgcgggt tttattttca gcctgtatca
      181 tagctaaaca aatcgagttg tgtgtccgtt tttgggcgtt ctgctagctt gtttaaagtc
      241 tcttgaatga atgtatgttc taagtcaaaa aactctgaca gcgcctttat at
//
LOCUS       DACEQH010000230          291 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.246, whole genome shotgun sequence.
ACCESSION   DACEQH010000230 DACEQH010000000
VERSION     DACEQH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.246"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            76..>291
                     /locus_tag="IW833_002879"
     CDS             76..>291
                     /locus_tag="IW833_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1308901.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIF"
BASE COUNT          111 a           55 c           50 g           75 t
ORIGIN      
        1 agacaaataa aaaagccatt tatgttacac tcaaattgta atacccgcta aagcatacaa
       61 ggagagtgaa cataaatgac ttatacccat cttacaccaa acgagcttgt aatgatagaa
      121 gcatattttc atcaagaaac tccggttgct atcgttgcga agcagcttaa acgtggacgc
      181 caaacaattt acaatgtcta taactttctc aaatgtggtg gaacagcact tgaatacttt
      241 gaacaataca aagaaaataa gcgacgttgt gggagaaccg aaatcatttt t
//
LOCUS       DACEQH010000231          281 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.247, whole genome shotgun sequence.
ACCESSION   DACEQH010000231 DACEQH010000000
VERSION     DACEQH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.247"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>281
                     /locus_tag="IW833_002880"
     CDS             <1..>281
                     /locus_tag="IW833_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAQ1308902.1"
                     /translation="IPPEPTKEKSYGNSQVLPRDYTRRNEIELVVKEMAEQVAIRIRQ
                     HNCKTGCVHLHIGTSILETRPGFSHQMKIPITDNTKELQNYCLFLFDKY"
BASE COUNT          104 a           50 c           58 g           69 t
ORIGIN      
        1 atcccaccag aaccgacaaa ggaaaaatca tatggaaatt cacaagtgtt accacgagat
       61 tatactagaa gaaatgaaat cgagctggtg gtaaaagaaa tggcggaaca agtagcgata
      121 cgcattaggc agcataactg taaaacaggt tgtgtccatt tacacattgg cacgtcgatc
      181 ttagaaacga gaccagggtt ttcacaccag atgaagatcc ctatcacaga taatacaaag
      241 gaattgcaga attattgtct tttcttgttt gataagtatt a
//
LOCUS       DACEQH010000232          279 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.248, whole genome shotgun sequence.
ACCESSION   DACEQH010000232 DACEQH010000000
VERSION     DACEQH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.248"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>279
                     /locus_tag="IW833_002881"
     CDS             <1..>279
                     /locus_tag="IW833_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1308903.1"
                     /translation="DRRLYKLTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNA
                     EIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKL"
BASE COUNT           86 a           68 c           50 g           75 t
ORIGIN      
        1 aagatcgcag actttacaaa ctaactcaat acttcctgcg ctttcctaga aaggcccgtt
       61 taaaggtcaa gtacctagtg atggacatga atgccagcta ttgtcagttg cttaaaacag
      121 tctttccaaa tgcggagatc gtaactgacc gctttcatat tgtccaacac atcaatcgca
      181 gcttcaatca attacgtgtc cagatcatga atcgttttcg aacctcacac tctgaaaatc
      241 agaagaagta ccgtcggctc aagcgatatt ggaaactct
//
LOCUS       DACEQH010000233          276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.250, whole genome shotgun sequence.
ACCESSION   DACEQH010000233 DACEQH010000000
VERSION     DACEQH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.250"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT           96 a           39 c           54 g           87 t
ORIGIN      
        1 cgactccttt ggtagggtga tgttttggtc gactaaattt taccaaaagt gagtctcttt
       61 ttgagttgaa acgaagaaaa agtgttagta gaaaatcgcc ttcgattttc caacaacaca
      121 aaatattata gaagcctttt ttctaaagga tttcaaggtg tctgtttgca tttattaaaa
      181 aaaagaaaaa gtattggcaa cagaaggcgt caccaacact agatattata gagccataat
      241 atgctgtata atatctagtg tataggctta agtgga
//
LOCUS       DACEQH010000234          254 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.251, whole genome shotgun sequence.
ACCESSION   DACEQH010000234 DACEQH010000000
VERSION     DACEQH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.251"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            46..>254
                     /locus_tag="IW833_002882"
     CDS             46..>254
                     /locus_tag="IW833_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308904.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNA"
BASE COUNT           96 a           36 c           62 g           60 t
ORIGIN      
        1 gcacacttgt tggtggtcat gcgggcggaa atgggggaat taaaaatgat tgattacaat
       61 gatcgtggac gtttaaagtg ggcagggttt tatttatctg atcatactga agaaattgat
      121 gccgacaatg aacaacgaac cagtcaaaat ttagcaaaag aacaaatgac gactgaagag
      181 attgcggaag tattgaacgc tgcagtcata aaaaatagat cagtatctat tcaaaaagaa
      241 gaacgaaacg ctga
//
LOCUS       DACEQH010000235          253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.252, whole genome shotgun sequence.
ACCESSION   DACEQH010000235 DACEQH010000000
VERSION     DACEQH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.252"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..159
                     /locus_tag="IW833_002883"
     CDS             <1..159
                     /locus_tag="IW833_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAQ1308905.1"
                     /translation="IKNHEKFLIRNYKISGGGCYLLECRFTSKKSMNSFLEQLNEYAN
                     YKLSIVID"
BASE COUNT          107 a           28 c           37 g           81 t
ORIGIN      
        1 attaaaaatc atgaaaaatt cttgattaga aattataaga ttagtggagg gggctgttat
       61 cttttggaat gtagatttac ttcaaaaaaa tcaatgaaca gctttttgga acagcttaat
      121 gaatatgcga attataaatt atctattgtt atagattaat aataaaacta gcagatggta
      181 aaaatactaa tatgcgagac acacattcaa ccataactat ataacttatc aatttaaaac
      241 taaactgatg gaa
//
LOCUS       DACEQH010000236          251 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.253, whole genome shotgun sequence.
ACCESSION   DACEQH010000236 DACEQH010000000
VERSION     DACEQH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.253"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>251
                     /locus_tag="IW833_002884"
     rRNA            <1..>251
                     /locus_tag="IW833_002884"
                     /product="23S ribosomal RNA"
BASE COUNT           67 a           49 c           79 g           56 t
ORIGIN      
        1 catttaaaga gtgcgtaata gctcactagt cgagtgaccc tgcgccgaaa atgtaccggg
       61 gctaaacata ttaccgaagc tgtggagtac acctttaggt gtattggtag gagagcgttc
      121 taagggcgtt gaaggcagat cgtgaggact gctggagcgc ttagaagtga gaatgccggt
      181 atgagtagcg aaagacaggt gagaatcctg tccaccgaat gactaaggtt tcctggggaa
      241 ggctcgtccg c
//
LOCUS       DACEQH010000237          251 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.254, whole genome shotgun sequence.
ACCESSION   DACEQH010000237 DACEQH010000000
VERSION     DACEQH010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.254"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>251
                     /locus_tag="IW833_002885"
     CDS             <1..>251
                     /locus_tag="IW833_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1308906.1"
                     /translation="LIEKEYKGVQAQFIKGKLDDSPSYCEHCRSVRIIRNGSYTTRTQ
                     MLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQL"
BASE COUNT           80 a           48 c           49 g           74 t
ORIGIN      
        1 ctgattgaaa aagaatataa aggcgttcaa gcgcagttta ttaaaggaaa attggatgac
       61 tctccttcct attgtgaaca ctgtcgatcg gttcgtatta tccgcaacgg ttcctatacg
      121 acacgcactc agatgttgaa agtcaaagaa aagttaacta ttttagaatt aaaacgtacc
      181 cgttttctct gccatgattg tggaaggacc ttttctgcga aaacggactt agttgatgaa
      241 catcatcaac t
//
LOCUS       DACEQH010000238          250 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.denovo.255, whole genome shotgun sequence.
ACCESSION   DACEQH010000238 DACEQH010000000
VERSION     DACEQH010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.denovo.255"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>250
                     /locus_tag="IW833_002886"
     CDS             <1..>250
                     /locus_tag="IW833_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1308907.1"
                     /translation="SGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAF
                     QLVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPC"
BASE COUNT           70 a           57 c           45 g           78 t
ORIGIN      
        1 agcggctact acgtcaaagg cgaacgagaa aaacaatttg cttattctgc ccatacttca
       61 tgcgacgaca atggatttat tcttagcaca atcatcactc ctggaaatat tcatgacagt
      121 caagtcgcct ttcagctcgt caaacaatcg aagagacttt ttcctgaaat caattgtgtt
      181 gtcgctgatg ctgggtataa gacacctaag tttgttcact ttttgactca tttgaacctt
      241 cgaccttgtt
//
LOCUS       DACEQH010000239        18844 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.guided.050, whole genome shotgun sequence.
ACCESSION   DACEQH010000239 DACEQH010000000
VERSION     DACEQH010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.guided.050"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            263..1159
                     /locus_tag="IW833_002887"
     CDS             263..1159
                     /locus_tag="IW833_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1308908.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1309..2250)
                     /locus_tag="IW833_002888"
     CDS             complement(1309..2250)
                     /locus_tag="IW833_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1308909.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2376..3497
                     /locus_tag="IW833_002889"
     CDS             2376..3497
                     /locus_tag="IW833_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1308910.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3755..5257
                     /locus_tag="IW833_002890"
     CDS             3755..5257
                     /locus_tag="IW833_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1308911.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5324..5851
                     /locus_tag="IW833_002891"
     CDS             5324..5851
                     /locus_tag="IW833_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1308912.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            6062..7123
                     /locus_tag="IW833_002892"
     CDS             6062..7123
                     /locus_tag="IW833_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1308913.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7126..8022
                     /gene="murQ"
                     /locus_tag="IW833_002893"
     CDS             7126..8022
                     /gene="murQ"
                     /locus_tag="IW833_002893"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1308914.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            8036..10018
                     /locus_tag="IW833_002894"
     CDS             8036..10018
                     /locus_tag="IW833_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1308915.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10083..10529)
                     /gene="nrdI"
                     /locus_tag="IW833_002895"
     CDS             complement(10083..10529)
                     /gene="nrdI"
                     /locus_tag="IW833_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1308916.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10740..12164
                     /locus_tag="IW833_002896"
     CDS             10740..12164
                     /locus_tag="IW833_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1308917.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12345..13118)
                     /locus_tag="IW833_002897"
     CDS             complement(12345..13118)
                     /locus_tag="IW833_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1308918.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(13115..14020)
                     /locus_tag="IW833_002898"
     CDS             complement(13115..14020)
                     /locus_tag="IW833_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1308919.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14174..14494)
                     /locus_tag="IW833_002899"
     CDS             complement(14174..14494)
                     /locus_tag="IW833_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ1308920.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14968..15654
                     /locus_tag="IW833_002900"
     CDS             14968..15654
                     /locus_tag="IW833_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ1308921.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            15656..16600
                     /locus_tag="IW833_002901"
     CDS             15656..16600
                     /locus_tag="IW833_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ1308922.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            16799..17443
                     /gene="pcp"
                     /locus_tag="IW833_002902"
     CDS             16799..17443
                     /gene="pcp"
                     /locus_tag="IW833_002902"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ1308923.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVSFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            17522..18799
                     /locus_tag="IW833_002903"
     CDS             17522..18799
                     /locus_tag="IW833_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308924.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6058 a         3321 c         3994 g         5471 t
ORIGIN      
        1 cctctttctt ttaaattcac aatcaaaatt tatattaaga ataaatagca atatttaaca
       61 atgactaatg ataacaaatt ttcagacaat tacaactatt cagaaaactt ttaggcgaat
      121 tttaatagaa gaatgattta tttcatcata ttttcataca aatgttaaat tttcatacaa
      181 atataggaaa cgttttactg gctttttact atttaaacag gctatcatag gaaaaagtga
      241 cagagaaagt gaggcggaaa agatgaacaa aaatgacgta ctgctagttg caaaaaataa
      301 atcactttat tttttgcctt ccatccgctc tttagatgtt tggtccggaa aagatttact
      361 acagtcggaa ttcattaaat cagctagaca acagttggca aattatcagc tgatcgtttt
      421 tttagattat ggatttgata tggagatggc tcgacgcgtg cgcccattta ccaatgcaaa
      481 aattgtgtta tttttttgga atcacttcaa agcagaacac gaggcgatgc tgaagcaggc
      541 acaagatgaa ccggcaatcg atgagattta tcattttgat tttttggaag caaaagaact
      601 tgggctgaaa cataatagtt cattttattc gaaacatatg aatttttctt ctagcgaacc
      661 aaagacggat ctattttttg gtgcaacgaa taatgggaga aaagaacgag cagaaagcta
      721 taagcaggaa tttataaaaa gagatgttac gaccaactat ttcattcttc cacatcgagg
      781 gaatgaacaa cctggatatc ttacttatga agagtacctg aaaaaaacag gggaatcgcg
      841 cgggattctg gaactgttgc gggagggtca gcaaggagtg actttgcgga catttgaatc
      901 catgtatttt caaaagaaac tagtgacgga taatcaagcg attgctcatt atcattttta
      961 tcgtcctgaa aatatttttt tgcttcgaga acgtgattta gatgaattac cggaatttct
     1021 gcatacacca tttaagccaa tcctatcaga gctattggaa ttttatgatg cagaaaattg
     1081 ggcaaaacga tttatcacta acgattccac tatttatgag caatacgaat atgacctctc
     1141 ctctgttgaa aacaaataaa aaacctacat tttgaagaga ttatgtactg aaacaaacaa
     1201 gcacaattct tatggcatca tgagagttgt gttttctttt tgatctttta ttgggcttaa
     1261 aaaaagagaa ccagctgctg attcagctga ttctcatcag agagcctttt attctgcgcc
     1321 aaggattcct tcttttgcat ggactaattg ggtcaaaaag gcacaatatg gtgtagcaat
     1381 atcatatttt tgtccttttc gccaaatggc accattgatg tagtcgattt cagtcaaacg
     1441 atggtttttg atcaggtctt ggtacataga tgggtaatgc aacccgatgt tgtcaggatc
     1501 ataagtagaa gcaatatgtt ggtacacttc ttcttgatct agtatgattc cttcttttgc
     1561 tgcgatagaa gcaaattcag atacgattgt tcgaaccatg ctctctgccg ctttttgcgc
     1621 gcctagttca gcaatattac aatccaaaat cgtgcataaa ccattcaatg ttccatttac
     1681 acaagctttt cgccaaatgg aataacgaac gttatggcta tagatcggat ttaaatttgc
     1741 ttcttgaaag acttctacaa ctttttttgt aaatgcttcg ccttctggtt ccaagttctc
     1801 tagttcgatt tctccatcac ctaacaatgt gacttttccg ggaccagcta gaccagctgt
     1861 ccacatcgtg atcccaaata agatattttt ctttggcaca tatttttcca gtacatcttc
     1921 atgacctaat ccatttagta aacacaaaac ataagtattg tcagtaataa tggattggat
     1981 cgaacaaaac atatcatcta attgattggc ttttgttaat gctacgatca agtccacgtg
     2041 ttcgttgctt tcgatgattt cttctggtga atagattggc agttttgcga ccacttcttt
     2101 accgttaaag tcagcaatca acccatttgt acgaatggct tcaatgtgag caggccaacg
     2161 atcgatcaag aggacctcat ttccgctttg atgtagcatc aatccgattc ggctgcccat
     2221 tgctccggcc ccagctattg cgattttcat gttttttctc tcctttattc aatgagttcc
     2281 cttttagtat atagaaaaat ggaagaaaat agcattgttt tgtttgaaaa aaatgaaaaa
     2341 aagtgtctta cttaagaaga aagtgaggga tacagatgga tattttggcg gcaattgatt
     2401 catttaaagg aagtgcttct tcatttgaac taaatcaggc agttctaaat ggctgggaag
     2461 atcgtaaagg aagaaaaatc aatgtgccga ttgcagatgg cggagaaaat actgcagaag
     2521 caatctatta cgcgctcagg ggaacatggc gtaccattga tgggatggat ctcttattcc
     2581 gtcagcgaaa aatcagctat ttattgacaa cttggcaagg aaaaaacacc gcagtcatcg
     2641 aatctgcaca agcattgggg ttagatctac ttgataaacc aacggatcaa actatccgac
     2701 ttgcttccag ttatgcctta ggagaactta ttaaagatgc actgcttcaa gaagtagaac
     2761 aaattattgt cactttagga ggaagcgggt gtagcgatgg tggtctagga ttactccaat
     2821 ccttgggtgc ttctttagaa gggtacacag aaggaaatcc tttattatct actaaaaaac
     2881 ttcattttga tcgaaaaaac gttcttgttt ctaaagtaaa attgattatc gctgcagatg
     2941 ttaccaatcc atatacaggt caacaaggag ctgcttggca atttggaaga caaaaaggag
     3001 gaatagatac gacgctagct ttcttagata aacaagcagg aaagattgct cagcaaataa
     3061 aagatttctg ggaggtggat ttagatgata ttccaggaag tggagcagca ggcggtattg
     3121 gtggagccct ttttttgttg ggagcgaaga tggtttctgg tttcgattta gtcagccaaa
     3181 tcactggatt ggaaaaaaag atcgaacaag cagatgtcat cattacagga gaagggcgga
     3241 tcgatcagca aacgatccac ggaaaagttc catacggtgt agcagtgctt gcaaaaaaat
     3301 atcgaaaacc aataatcgct atttgcggaa gtagagacca agagctagaa ggactttctg
     3361 attttctccc aattgtctta agtattcatt tgggtccaat aggtttagaa gaagcaatgg
     3421 aacacaggca aacattagaa aaaacaaaaa tcttaggaca gacacttagc agatttttca
     3481 ccatcctccc gaagtagttg acaaaatcat tttctttatg ctatcgtaat ttcaaatata
     3541 ggacaaagtg agggatccgt gcattgaaaa attaagtcga ttgatgaaag ctgcattcag
     3601 actgtacctt gtggagacaa ggaagcagtg tgcagatttt cgggattggc ttggtttttt
     3661 cgatgtatgg gtctttttgt gtgtatccaa cccttgcatc ctctttagcc agttctgaaa
     3721 ataggactgg tcttttttta cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct
     3781 gacattcggc tacgacagcc aaggcacatt attatttgaa caagcaaatc taaattttga
     3841 cacacaatgg aaactaggac ttatcggacg aaacggtcga ggaaagacaa ctttactgaa
     3901 tattctacaa aacaaactac cttatcaagg gcaagtaatc catcagcaag aatttgccta
     3961 tttcccgcaa cagacaaaag ataaagaacg tttaacctat tacgtgttaa atgatattac
     4021 ggattttgag atatgggaaa tcgaaagaga gctccaattg atgcaaacag atcctgaaat
     4081 cttatggaga gaattcagca cactatcggg gggagagaag acaaaagtcc tactggcact
     4141 tttatttgtg gatgacactc atttcccgtt aatcgatgaa ccaacgaatc atttggatat
     4201 ctctggtaga aaacaagtag cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt
     4261 cagccatgac cggggattta tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag
     4321 tcaactggaa ctttatcaag ggaatttctc tatctatgaa gaacagaaaa aacttcgtga
     4381 tgaatttgaa atggctcaaa atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac
     4441 agcagctgaa aaagccgaat ggtctcgttc ccgagaagga gataaaacaa agaaacaagt
     4501 cggattcatc gatactgaat ctagacgagt gaataaagga gcagtgggtg ctgatgctgc
     4561 acggacgatg aaacgatcca aagcaatcgt gaatcggatg gagacccaga tcagcgagaa
     4621 agaaaaacta ttaaaagata tcgaatatat cgattctttg acgatgaata gccaagcgtc
     4681 tcaccataag cgacttttaa gcgtagaaga tcttcaatta gggtatgaaa atctgttatt
     4741 cgagccaatt cattttacaa tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg
     4801 tgcaggaaag tcatccatta tccattatct tctgggggca ttcaacggca aggttatagg
     4861 agaaaaatac cagccaaaac atctgagcat tagttatgca agccaaaatt atgaagacaa
     4921 tcgaggaacg ttggcggaat ttgcagagaa aaaccaagta gactaccaag catttttgaa
     4981 caacctccga aagcttggga tggaaagaga tgtttttcat aacaagatcg agcagatgag
     5041 tatgggccaa cggaaaaaag tggaattggc taaatcttta tcacagccag ctgaactata
     5101 tacatgggat gaaccattga attatttgga tgtcttcaat caagaacaat tagaacaact
     5161 gatcttgaac gtgaaacctg ccatgttact agtggaacat gatcaaacct tcctggataa
     5221 agtatctact gagattattt ctcttgagag aatctaaccc ttgtaatctc aagaacaaca
     5281 aggtatggtt aaggggaact ttgccaacga aggagagaat cacatgcttt atgtaacgag
     5341 acacggagaa acaacttgga acgcacaagg attagtttgc ggacgggcag atgtgccgct
     5401 gactgaaaaa ggacagatgc aggcacagaa actagcagaa aaggtagtag atttgcctgt
     5461 tccaatcacg aaaatcatcc attcccctct tcaacgagca agagatacag cacaagcagt
     5521 tgctgatcgt ctcagcttgc cgctgaccgt tgatgaacga ttggtagaaa tggattttgg
     5581 ggattatgat ggactgccaa gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc
     5641 tgtacgattc ccaaatgggg agtcagtctt agatgtgtat gcacggatcg ttcctttatt
     5701 gaaagaatgc atcgaagacg aagaaaatgt ctatctgtta gtttgccata atgccttgat
     5761 aagagtgatc aatgcttatt ttcatccgat gcctaatgag gggttcttta cattcatggt
     5821 agataataca gaattggttt cttatgagta attatgtttt tgtgttattt aaataaaata
     5881 tttcaaggca ctgcttttgc ttagcagtgc cttttttagg ttatgacaat tcttttgata
     5941 atataaactc aacctttaga aatgaaattt aattatttta ataaaagtat tgaaattttg
     6001 tttctttaga tatatactct agtcatagaa atgaagacgg atacagaaaa agagggataa
     6061 tatgtttggc ttttcaatat ttatgaacga ttctttgtca gaagagaaga aacagtatat
     6121 ccgccaaatg gcatccaatg gctttgtcgg tattttcact tccatgcata taccagaaga
     6181 tgatgtttcg gcatatcaaa agcgattgat cgaactagga agcgaagcga aaaagaataa
     6241 actagaattg atggtcgata tttcaggaga ggcattggaa cgagcgggtt tttcattaga
     6301 agatctaacg cctttgaaag caattggtgt aacaggttta agaatggatt atcatatttc
     6361 caatcagcag attgccgact ggtcacatca attgaaaatc agtttgaatg ctagcacgat
     6421 cacgcctaaa gatatcgatg aattgaaaga agcagaagct gatttctcac agattgaagc
     6481 ttggcataat tattatccac gtcccgaaac agggctggac aaagaatggt atcagaaaaa
     6541 aaatcaatgg ctcaaaattc aaggtctcct tgtccaaggt tttgtaccag gagatacaga
     6601 gttgagaggc ccactatatc aaggattgcc aactttggaa gaacatcgag ggatccatcc
     6661 tcttgcaggc gctctagatc tattagcaag taacacggat attgtttaca tcggagatgc
     6721 aggtctgtca gaaaacgttc aggagcaatt tgcttctttc caaaaagaac agacagtctt
     6781 gctccatacg gaacctgtag atgaagaatt ttacgaatat atattaggga agcatacgaa
     6841 tcggcaagac gatgcaagag atgtcatacg aagcgtagat gcacgttttc gagagattcc
     6901 gcctatccca gcaagaaata cagctacgcg tatgaaaggg tctattactt tagataacga
     6961 aaagtatttg cgctatatgg gtgagatcca attaaccaaa tatgatttgc cagcggatga
     7021 aaaagtgaat gtagtagcta aagttatcaa agaagagcta ccattgatca accaaatcaa
     7081 agctggtatg aactatcaat ttataagaaa agaaggaaga taaaaatgag caaaatcaat
     7141 ttagagaacc taacaacgga acgcagaaac caagaaactt ttggattaga tgagatgagt
     7201 atttctcaag ccgttcaact aatgaacaaa gaagatcaga aagtggccgc tgcggttgaa
     7261 aaagaactga caagtatcga accagtgatt gctaacacga ttgagtcctt taaaaaaggt
     7321 ggacgtctga tttatatggg agccggaaca agtggaagac taggtgtgtt agatgctgct
     7381 gaatgcgtac cgacattcgg tgtagaaccg gaaatggtca taggtctaat tgctggtgga
     7441 gaagaagcaa tgactgtagc tgtcgaaggg gccgaagatt cttatgagct agggcaagaa
     7501 gatctaatca agctgaattt gacaaaaaat gatatagtta ttggtatcgc agctagcggt
     7561 cgtacaccat atgtgatcgg tggattagat tacgcaaaat cgatcggtgc tgtcacagga
     7621 acgatttctt gcaataaaca agccgaaatc agtaaacacg cggatttccc aatcgaagtc
     7681 gatgcaggtc cagaattttt gacaggctct actcgattga agtcaggaac agcacaaaaa
     7741 cttatcttga atatgatttc tactatcagt atgattggta taggtaaagt attcaataac
     7801 ttgatggtcg atgtcaaacc aacaaatgaa aaactaatcg aacgctcaaa acgaatcatc
     7861 atgcaagcga cagaagctga ttacgaaaca gctgaaaagt actttgaaga agcagatcaa
     7921 aatgttaagc tggctatcgt catgattttg acaaatagtg ataaagagga agctcaagca
     7981 aaattagctg aagcaaacgg atttgtaaaa gggaccttat gagggggaaa agaccatggc
     8041 aaaaaacgaa ctgactttgg aagaactggc aaaacaaatt tataaagggg caggcggcat
     8101 gggcaatgta gaaagcattg tccactgcat gactcgtgtg cgtatgaaga tcatcaatga
     8161 tgatcttgta aatgtctctg aattaaaagc gattccagga gtgcttggtg tagtagaaga
     8221 tgaacagctt caagtcatca tcggacctgg gaaagtgaac aaagtagcac agcaaatggt
     8281 tgatttggca ggcgtgaaat taggagaaaa actttcttct gtaagtacag ctacagcaaa
     8341 ttcatcagca tctggaaaag accgtgtaaa cgaacgggca caagaaatga aagcggcaca
     8401 aaaatcgaaa caaaaaccat ctaaaatcaa atcggtatta aaagacattt ctaatatctt
     8461 tgtgccaatg attcctgcat ttgtcggtac tgggatcgtg gcagggattg ccgcagtttt
     8521 agcaaactta gtcacagccg gaacacttga tgcagcaaca tggcaacaat atatcgatgt
     8581 gatgaacatc ttgaaaaacg ggatgtttag ctacctagtt atctatacag gtatcaatgc
     8641 tgcacaagtt tttggtgcaa caccaactct tggtggagtt atcggggccg ttgtgatgct
     8701 gacagggatg aatccggaag caccactgaa aaacttattt actggtgaag ctcttgcagc
     8761 tggacaaggt gggatcctag gtgtcatctt cgctgtttgg ctattgtcaa tcgttgagaa
     8821 aaaactgcat aaagtagtac ctgatgcggt agatatcatc gttacaccaa ctctttcatt
     8881 gattgccatc ggtctgattg aaattttctt catcatgcct ttagcaggtt tcatttctga
     8941 tggcatggtc ggtgggatca actgggtact tggcgttggt ggagcatttg ccggcttcgt
     9001 tctaggcaca ttattcttgc cgatggttat gttcggactg catcaaattt tgactccaat
     9061 ccatgtgcaa atgattgatg aaactggtcg tacgctattg cttccaattc ttgcgatggc
     9121 tggtgcagga caagtgggtg cagcattggc attatggatt cgctgcaaaa aagataaaga
     9181 attaacagaa atgatcaaag gagctttacc agtcgggatc ctaggaatcg gcgaaccatt
     9241 gatttacgga gtaacattac ctctaggacg cccatttatt actgcttgta tcggtggagg
     9301 tatcggcggg gcagtgatcg gaatgatcgg aaatgtcggg gctatcgcta ttggaccttc
     9361 aggagccgct ttgatcccat tgatctctga cggtaaatgg ttaggctaca tccttggatt
     9421 attggcagct tatgctggtg gcttcgttgc tacattcttc ttcggtattc ctaaagaccg
     9481 tttagaaaaa gaagaattag cgactgaaac agccacagta aatgaagtgc ctgaaaaagt
     9541 agtacctcaa acttcagctg aaagtgtgac tatcctctca tcagttgctg acggaacagt
     9601 tgtttcttta gataaagcaa gtgatccagt ttttgcacaa aagatgatgg gtgaaggcta
     9661 ctttgtagaa ccagaaaacg gacatatcta ttctccagtt actggggtag tcagctctgt
     9721 cttcccaacg aaacacgcta ttgggatcac aacgccaaat ggtttagaaa tcttgcttca
     9781 tatgggaatc aacacagttg acttaggcgg tacgccattt gatgtgaaag taacagaagg
     9841 gcagcaagtg acacctgata cgcttgtagc agaagctgac ttagaagcga tcaaagcagc
     9901 aggcaaagaa acatccatga tcgtcttggt aaccaatatg gatcgtgtaa aaaactttgt
     9961 cttggaaaaa acaggtaaag caaaagcaaa agcacctgtg atggatgtca aagcttaata
    10021 tgatagaaac aatagaaacg aaagaagaaa ctgaaatcta tttttcagct tcttcttttt
    10081 tattatttat tgttttctgc taaaatctga taaattcgtg tcacatcaga tggtgttccc
    10141 cgtaattcat aatctgccaa gaaaggaaag ccaaatgttt gtgcatattg tttggctgtt
    10201 agacaatact gattattgaa gtttttattt ccgctaccga ctacaccaag acaaagttta
    10261 tgattgtctt catgttccag atattcacgc atcgtttcgg tcaagatttc tgtatcaccg
    10321 ttatctaaac catttccgcc gtctaaataa gtaggaacga aagtaaaaaa tggttctgtt
    10381 tctttcgtaa aatcactatt ttcatggatt tcttttaaag taacttcagg attgacagga
    10441 tcttctgcat gcattttttc agcatattct gctagatgtt tggcaaaggc acgcgtattt
    10501 cctgaaatcg atatatataa aatgttcatg gatcatcctc caattctttc cttttagtat
    10561 agcatctttt ttaagttttt tggtggtgta atttggaaag ggactaggcg ctttgcttgt
    10621 ataatgagaa aaaagccggt agaattatat ttctattaaa gtttttatta attaagtaaa
    10681 aataaaaaag gtttgttata ctttttgata aaagcggttt tattttggag gatgcttaca
    10741 tggatacaga gcaacagaaa aaaggaattg gtttaattcc acttgctgcc ttagtaattg
    10801 gttcagcgat tggaggtggc gtatttggta ttatgacgga catctccgga tcggctggcg
    10861 gcccagctat tttcagttgg gtactcgtag gaacagcaat gttgatgctc tccttgtcga
    10921 tcaataatat caataaaaaa cgtccagatt taaaaggcgg gatctttagt tacgcagaag
    10981 aaggttttgg ccgtttcgct ggttttatca gcggttgggg ttactggctc acatgctggt
    11041 taggcaacgt agcttttgca acattgttga tgagcgcatt aggatatttc ttcccagtat
    11101 ttgaaggtgg gcaaaatctg gcatcgatcg tgatgagtac agtcttttta tggggatatg
    11161 catggctagt gaatcgggga gtcgaaaatg ctagtttcgt caatgctatc gtcacattct
    11221 gtaaattagt gccattattt ttatttattg tagtagctat cagcacgttt tcgatccaac
    11281 aattcattga taacttttct agcgacatta tattgaatgc gaatggacag ccgatcccgt
    11341 tttccagtca agtattgagt tgcatcatgg tgatgctttg ggtatttgtt ggtattgaag
    11401 gagcctctgt cgtaggttca cgagcaaaaa aacaatcaga tgttgggaaa gcaacgattc
    11461 tagggatatt cggcttgatt attatttacg tcttggtttc catgcttcca tatgggacaa
    11521 tgacgatgaa tcagctgcaa acgatcaagg cgcagcctgc tatgggctat gtgtttgaaa
    11581 tgatggttgg taaatgggga gcagtcgtga tcaacggcgg tttgatcatt tctttagttg
    11641 gtgtttggct ctcatggacg atcttaccga ttgaaacaat gggaaacatg gctgaagacg
    11701 gacttttgcc taaagcatgg ggaaaactca acaaaaatgg ggcaccaact tatgctatcg
    11761 tattgacgac gttatgtacg aatgtctttt tactttcatt attattcact gacagtgctt
    11821 ataactttgc ttacacactt ggtacagcag ctattttctt cacgtggctt ttcttaggat
    11881 tgtaccaaat gaaactttct tatcagcgga aagaatgggt ccaattttta gtagggcttt
    11941 tagcaagtgc ctttcaagta tgggctatgc tagtcgtagc ctttaatgaa gtaatgcttg
    12001 ttcttgttct tttcttgcca ggcatttatt tttacctcaa agcacgtaag gagcaaaacg
    12061 agcctgtaca acatatccac ttagataaga ttttacttat aggtgtgacg atggtcggag
    12121 tgattgcaat ggttttattt gccactggga tgcttcacgt atagaagtat aaaagaagta
    12181 taaagagagg ttgtgagcct gtcgtttatt caaaaacata ctaggaaagg agctgtgaca
    12241 caaatgttgt cacagctcct ttttcagaat aaacggtgtt taagagacag ccaatcggca
    12301 ataaggctga acgtctcttt cacagcctct tttttgatga gtcatcatac attttttcgc
    12361 ttaaatacga gatatcctaa tgctaagaac aaaacgatgt aagctaaatt accgaatagc
    12421 atttgattag tagaaagatg agtcaattgc ttcatgatct cttcgtttcc aatttggttt
    12481 tgcagattca acatactgat cggattccat ttgatccact cccatttttg gatgagtgca
    12541 ataaggatac cagatatgat tgacgaagca aaataaaaga cgattcctgc tgagatggat
    12601 gcgccggaac tattgataaa gcaagcaatc atcaacacta aacttaaaat cagccataaa
    12661 ccgatataac tacccaaagt ataagtgaac aagctttgga tcagtgtttg accagtagaa
    12721 accttctccg tgaaggaaat cgaagggaaa agaatcagtt tcatcaacac agtgacaata
    12781 atagctaaaa ggtataaata aacagaataa ataactaatg tgatccattt gcttactaat
    12841 atttctcctc gagaatactt tcgataaagg agatttttca atgtaccata ttgtgcttcc
    12901 atcgaaatga tcgtgcttgc agctgcaatc atgataaaga caatccaaga tgtggcacta
    12961 aaaagctgag cagtcatttc aactggatca atgatcgtcg tgtatttttt ggctagaagt
    13021 gctgttccta tgagcaaaac aactaataga acagataaaa cagcagttga ctttttcttg
    13081 atcaatttaa ataattcttg ttttacaagc aatgtcattt ttcttcttcc ccctttactg
    13141 aatccagaat ttccaataga gaagattcca aatcattcgt cagatgctgg acgtctgtca
    13201 catggatatt ttgtgcagac aatagctgga tcacggttcc taataattct tcgttttctt
    13261 ttaaaagctt gattttatca ggatcttcca aagtatatcc agcttctgtc aagatcgttc
    13321 gagcagctag atcatttgtt gtagagataa cgatgaaatc ttgcgctgtt tggttcaatt
    13381 cagccattgt acattgtttg atgatctttc cttgattgat caggatcagc tcatcgacca
    13441 ttttttcaag ctcgctgagt aagtgactgg agatcaagaa agaggtgccc tgagtagcca
    13501 agtcggtaat gatttttcgc acatctcttg ttgcttgtgg atccaaaccg ttcatcggct
    13561 catctaaaat aacgatagac ggatggttca gtaaggcaag agcaattccc aatttttgtt
    13621 tcattcccaa cgagtaggat ttcgcttttt tgtgaatgta cttttccatc ttgagctgat
    13681 tcacgatagt gtcgatagca gatacatcgt tggtttcaga gaataacttc agatgatcat
    13741 agcctgttaa gaatggatag atacccgggt attcgatcaa tgctcctact tgttccaacc
    13801 ctttatgact agagggagaa atctcatttt caccaataca gattgttcca tctgagtaat
    13861 ggatcagtcc taaaatagct ttcatgatcg ttgtttttcc agcgccatta ggacctacta
    13921 atccaacgat tttgccaggg gataagctaa aactgacatc gctgagggct tgtttgtgac
    13981 caaagttctt ggataattct tggaccgaaa gacttttcaa aaatattcac tccttttcta
    14041 tagatagtac aagcatacac ctgatgagtg gagaagtcgc taggtcattg gacttatttc
    14101 ttattttttc ataaaaaaat tgaatattaa ataaaaaggc ttattttttc tgatcagtac
    14161 ttattccaag tgtctagtgg ccgctagtaa tcttgatgcc aatgatactg atgagtagca
    14221 tccccacaaa gaaccaagtg gaaagattca gcttatcatg aaacaagacc acaccaatta
    14281 tgaccgaacc aactgcgcca atccctgtcc agataggata agagagactc atcggcaaac
    14341 ttttgattgc ctttgacaaa aagtaaaaac tagcaatcat gccaatcact gtgtaaaaag
    14401 aataattgat tttagaaaaa ccatcactcc atttcatcat cgttgaccag aatacttcca
    14461 aaattcctgc tatgattaat tccatccatg ccatacttac attcctcctt aactataaaa
    14521 aaatacacaa aaagaggaaa atcatccctt caaagaccta atggaatgat catcctcttt
    14581 atcctacccg gtggcagatt gtatatcttt ttctgtagta tagcacacct atccagatat
    14641 cggcaatctc atacttttta gtaaagtgta ccaagcactg acggatatat cgttagcgga
    14701 aaagaagatg aatgctttgc aatttcatag aaagaagttg ttatttatta atcatttaaa
    14761 aaaaacagta actagacctt atttctatat attatcgggc aataaattaa aaaaagcttt
    14821 accttacaat aagtaagata atttttgtga taaaaaaata acgaaaaata tttaaaaata
    14881 gtgtctattt cgtgagaaaa caactatatt ttttatgtgt tttgctttat aattaaaaaa
    14941 atattgtcat tttatggagg aaaatatatg gaaaattatc ttagcctttt aggcgtcttg
    15001 attgttattc tcgggtttgc atttaaatta gactcgatat tgatcgtaat ggttgcttta
    15061 gttgttacag ctttgactgg cggtcttggc attcaaggaa tgttagaact tttaggtaca
    15121 agttttgtaa acaatcgcgg gatggcgatt tttatcatca ttatgttggc aacaggaacg
    15181 cttgaaagaa atggtctgaa agaatcagcg gcttccttga tcaagcgatt caagaaagta
    15241 tctgctggta tgattgtaga tatttatggg atctttcgaa tgatctttgc ggcttttaac
    15301 gtgagttttg gtggagttgc tggatttgtt cgtccaatca ttttaccgat ggcattgggg
    15361 accatcgaat caaaagggtt agaagtccat cctaagcatg aagaagaact aaaaggtatg
    15421 gcttcagcta tggaaaatgt ctgctggttc tttggccaag tactattcat cggaggagca
    15481 ggggcgttgc tagtccaatc aacactgaaa gatctaggat atgacgtaac attgggacag
    15541 cttgctttag tggaagttcc agtggctatc acggctttag ttgtggcaag tgtctacttc
    15601 tatcttaaag aaaagaaatt agcgaagaaa tattatccag gaaaggggca ataagatgga
    15661 cttttttaca aatcctgata ttttattagg cgataaatta ctggagatcc tttatatcgt
    15721 gatgggactc gtattgattt acactggagt aagaaattta acagataaaa ccaatcctca
    15781 tcgttatggg tcagcctttt tctggacggt cttaggggta gttattgcag gaggaagatg
    15841 gcttccggca atcgtgaacg gtggcttgat ctttgcgatg accataccag caattgctaa
    15901 gaaagtcagc aaaggagaaa gccggcttcc atcaaaagaa tatatggaaa aaatgtcaga
    15961 caaactagga atgaaaattt ttattccagc tttgagtatc ggtgtctttg cgatcttgtt
    16021 tgctctattt accaatttag gtgctttagt cggcgtgggc gtcggtgttt ttgcagccat
    16081 gctaatcttg atgttctttt ctcgagataa tcgaccaagt acatttttag atgatgcagc
    16141 agatatgctg ggcacagtag ggccgttaag catgttgcca atgcttttag cttctctagg
    16201 tgcggtattt acaagcgctg gagttgggac agtgatttcc aatgcagtag gtacgatcgt
    16261 tccagaagga aatgtgaaca tcggcatcat catttacgct cttggcatgg tgatatttac
    16321 ggtaatcatg gggaatgcat ttgcggccat caccgtgatg acggttggga tcggtgcacc
    16381 attcgtattt agtcatggag cgaatccagc gttaatagga atggttgcat tgacttgcgg
    16441 tttttgtggg accctgctga caccgatggc tgctaacttc aatatcgtgc ctgtagcgat
    16501 gctggagatg aaagacaaat acggagtgat caaaaaccag ttgtttattg cgttatttat
    16561 gctagttttc caaattgctt atatgattct atttaaataa tagaagagag gggttgtgac
    16621 agaataattg tcgcaacctc ttctaacatt gtagtggaac actatttttt tataagaata
    16681 tattaagttt gatctgttat gttatttaaa ctattgtttc ctttatttac gctgttcaag
    16741 gaaaatggta tgataaaaga taaccatatg ttaaatcaat cggacggagg aacaacagat
    16801 gaaagtacta gtaacaggtt ttgatccatt tggtggggac aaagtgaatc cagcctatga
    16861 agcagtaaaa aaaatgccag atgaaatcag cggtgctgaa atcatcaaag tggaagtacc
    16921 gaccgtattc gaaaaaagca gtcaagtagt caaagaagcc atccaacagc accaaccaga
    16981 tgtcgtgatc tgtgtcggac aagcaggtgg gcggtcagca gtctcgttcg aacgtgtcgc
    17041 aatcaatcta gcagaagcaa gaatccctga taatgaagga aatcagccat tcgatacggc
    17101 tttagaagaa aacggtccag ccgcttactt tacctcattg ccaatcaaag caatgacaaa
    17161 aaatgtacaa gatcatggtc taccagcgta catctcttat acagcaggaa catttgtctg
    17221 caatgatatc atgtatcgtt tgcttcattt gatcgaaaca caattcccgg caattcgagg
    17281 tgggttcatc cacgtgtcat tctcacccga tcaagtgatc gatcgtccag tgggtacgcc
    17341 ttctatgtct ttagaagaca ttgcttcttc tttgacttat gcagttgaag cagcagtaga
    17401 aaataaagaa gatatttccg gaaatgccgg aacaactcac taatttagca agaaacactg
    17461 gctggttgct aacgttatgt tagtagccag ctttttgtaa ttagaaagga aagaaacagc
    17521 cgtggaagaa tggcaatccg tctttgaaga atggtttcca aaagaaatca gcaaaagcta
    17581 tcccatcaaa atttctaaac aatacacaag cagtcaacgt tgggagatct atgcaaaatt
    17641 aacgaaaaaa cagcgggaac tagtcgataa gcaccgccga tatctaatca gctctcgttt
    17701 tatggaagaa cattatttag ctgcaacaga ttgggtgttt tctgacttca agatcaatcc
    17761 gttttttcga acaaaacgaa gccagcaaaa attgtattgt gagtgtggta gagaattaaa
    17821 agtgcaatac atcgttaaat cgccaaaaac cggaaagatc ctaaaactag gcatcaacca
    17881 ctttgccgat cacctgcatg tctctccgac agtggcagct tcgatccatc agggaatgac
    17941 aaaagttgat ctggccttag acgaattatt atggcttaag cagaagaata tcgattttcc
    18001 tgaaggacta tggcaaaaat actgttttgt cttgtaccaa aatcgtcgga tgaaacagcc
    18061 atatttaccg gatataaaat tagctcagcg gctcgcagaa tttcgccaag tagaaatgcc
    18121 gatttacata gcggattatc aagcattgga aaatgaaatc aagaaaataa gtgaacacat
    18181 caatgggcag tcaaaaaagc gacagataaa aaaagaacta tttgacgact ttgcagaaga
    18241 attagtaaag gatgtagaag aattcttgat caactatcgc gcatttttac gtaaagattg
    18301 gcaatccatc gtatatgaag aagtgccagt acatcctaat gcttattttg aaacatttat
    18361 ttccgtgttg agaaagacaa aacgtcaacg aacaccagaa gtcactgctc agatggaata
    18421 ttttgcgaaa aaccaacgat tcatccaacc aaaaatctat ctatttattt ggaagcaata
    18481 ttgtcgctat ggttttacag aagggttctt cgacagcatt cctcggatag tcagaaatgg
    18541 ttttttaaaa gtattgcgta aagaacgaga agctatacaa tctgctgata agaaagaccg
    18601 tacagtctca aaagaaaaat ggcaactagt agtgaaagat atccaatctg gaaatgtgca
    18661 agagacaatc gacaaatgga aaggaaaaca ttatcgtttt actgaagctc agaaacaagc
    18721 gttggaatat tatcagaagt tagaagaatc actgcgtttc aatgatgagg caagaaagta
    18781 tctgaaggaa ttactataaa aaatagaggc tgggacagaa gcgtttaact ccaagaaata
    18841 agaa
//
LOCUS       DACEQH010000240        11422 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.guided.075, whole genome shotgun sequence.
ACCESSION   DACEQH010000240 DACEQH010000000
VERSION     DACEQH010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.guided.075"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            39..977
                     /locus_tag="IW833_002904"
                     /pseudo
     CDS             39..977
                     /locus_tag="IW833_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            980..3163
                     /locus_tag="IW833_002905"
     CDS             980..3163
                     /locus_tag="IW833_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1308925.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3160..4161
                     /locus_tag="IW833_002906"
     CDS             3160..4161
                     /locus_tag="IW833_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1308926.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4176..5090
                     /locus_tag="IW833_002907"
     CDS             4176..5090
                     /locus_tag="IW833_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1308927.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5335..5451
                     /locus_tag="IW833_002908"
     CDS             5335..5451
                     /locus_tag="IW833_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1308928.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5467..7386
                     /gene="tet(M)"
                     /locus_tag="IW833_002909"
     CDS             5467..7386
                     /gene="tet(M)"
                     /locus_tag="IW833_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1308929.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQREVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7484..8857
                     /gene="tet(L)"
                     /locus_tag="IW833_002910"
     CDS             7484..8857
                     /gene="tet(L)"
                     /locus_tag="IW833_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ1308930.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9421..10683
                     /locus_tag="IW833_002911"
     CDS             9421..10683
                     /locus_tag="IW833_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ1308931.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10928..11410
                     /locus_tag="IW833_002912"
     CDS             10928..11410
                     /locus_tag="IW833_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ1308932.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNGSVAKF
                     "
BASE COUNT         3771 a         1907 c         2389 g         3355 t
ORIGIN      
        1 acttggagat aacatcaaaa agttacctat cgctataacg tggcagatgg aaagaaacct
       61 tgccagagat ttcccatgaa atcaatatcg tcaatctgac ttctgatgag aaaaacaaag
      121 gcttacttga cccttatgta attatgaaaa ataccaaaga ttctgaatca ctggctattg
      181 atattttgac attccttacg gggatttcct ctcgtgatgg ggaacgcttc ccaatcctta
      241 gaaaagccat tcgtgcagta accaatagtg aagtgcgagg gttgatgaaa gtgattgaag
      301 aattacgggt tgagaatacg ccactaagta ccagcatagc cgaccatatc gaaagtttta
      361 cagactatga ctttgcccat ctgcttttta gtgatggtta tgtagagcag tctatcagcc
      421 ttgaaaaaca actgaacatt atacaggttg ccgacttggt acttcctgac aaggaaactt
      481 cctttgagga atataccaca atggagttac tatcggtagc aatgctgatt gtcattagta
      541 cctttgcgtt ggactttatc catacagacc gaagtatttt caagattgta gacttagacg
      601 aagcatggag cttcttacag gtggcacaag ggaaaacact atctatgaag ctggttcgtg
      661 ctggtcgtgc tatgaacgct ggggtatatt tcgtgaccca aaatacagat gacctcttag
      721 atgaaaaact gaaaaataac ctcggcttaa aatttgcctt tcgttccact gaccttaacg
      781 agattaaaaa aacccttgcc ttttttggtg tagacccaga ggacgaaaac aatcagaagc
      841 gattgcgtga tttggaaaac gggcaatgcc ttatcagtga tttatatggt cgtgtcggtg
      901 tgatacagtt ccaccctgta tttgaagaac tgctccatgc ctttgatacc agaccacctg
      961 tgcgaaaaga ggtgtaaatg tgaaaccatc aatattaaac agaataaaat caaactggac
     1021 gctgaaacgt ctaggtaaag tggcaatgac agtggctttc acacttgtga ttgccatttt
     1081 tcttttagcc atgctgggaa cggtggttca agctgcgggc ttggtagatg atacggtcaa
     1141 tgtggcaaat gaatacagcc gatacccact tgaaaactat caactggatt tttatgtgga
     1201 taatagctgg ggctggctac cgtggaactg gtcggacgga attggaaaac aagtcatgta
     1261 tggactatat gccattacca attttatttg gacaatcagt ctttatgttt ccaatgcgac
     1321 gggttactta gtacaggaag cctattcctt agacttcatt tccgctacag cagattccat
     1381 tggcaagaat atgcagaccc ttgcaggggt tacgcctagt ggactatcaa cagagggttt
     1441 ctatgttgga ttccttttac tcttgatttt ggttcttggg gtttatgttg cctatacagg
     1501 actgataaag agagaaacca caaaggcaat tcatgccctc atgaattttg tgctggtttt
     1561 tatcctatcg gcttccttta ttgcctacgc tcccgactac attaaaaaaa tcaatgactt
     1621 ttcatcagac atcagcaacg ctagtttgtc actgggtaca aaaattgtca tgccccattc
     1681 agatagtcaa ggcaaggaca gtgtggactt gatacgtgac agcctatttt ccatacaggt
     1741 tcagcaaccg tggctactgc ttcaatataa cagttcagac attgaaagta ttggtattga
     1801 ccgtgtggaa agcctgctct caaccagtcc agattccaac aatggggaag acagagaaaa
     1861 aattgttgcg gaagaaattg aagatagaag caataccaat atgacgatta caaagacgat
     1921 taaccgttta ggtacagtct tcttcctatt tgtcttcaat attgggattt ccatatttgt
     1981 attcctatta acaggaatca tgattttctc gcaggtactt tttatcatct atgctatgtt
     2041 tctgcctgtg agctttattt taagcatgat tccatcattt gatggtatgt caaaacgagc
     2101 cataacaaag ctctttaata ccattttgac acgagctgga atcacattga ttattacgac
     2161 agcatttagt atttcaacca tgctctatac cttatcggct ggttatccgt tctttttgat
     2221 tgcttttcta cagattgtga cctttgcagg aatctacttt aagctgggcg atttaatgag
     2281 tatgttttct ctacagagta acgattctca aagtgtggga agtcgtatga tgagaaaacc
     2341 tcgtatgctt atgcacgctc acatgcaccg tctacagcgg aaacttggac gctccgtgac
     2401 tgctttaggg gctgggtctg ccattgccag tgctacgggt aaaaaaggac agtcgggttc
     2461 gggaagttct gcgagtacac aagcagatca ctctcgacca aacgggcagg aaaaatcaac
     2521 actaggaaag cgtatcggtc aaaccatcgg tacagtagct gataccaaag acagaatggt
     2581 agatactgct ggtaatttga aagaacaggt taaggatttg ccgaccaatg caagatatgc
     2641 agtatatcaa ggaaaatcta aagtgacgga taatgtccat gatttaacca gaagtatttc
     2701 tcaaaccaaa gcggataaag ccagtggacg caagaaacag caggaacaaa gacggaaaac
     2761 cattgcggag cgtcgctctg aaatggaaca ggtcaaacag aaaaaacagc cagcttcttc
     2821 cgttcatgaa agaccagcca ctaagcaaga acaatctcat gatggacaga cttcaaggca
     2881 aacaactgta caggcttcct atagggaatc tcaacaagcc aaacaagagc gtccaacagt
     2941 taagtccgat tcttcaagtt taaaaacgga acgtcaaagt aatacagttc aagaaagaac
     3001 ggttcaaaag ccagtaactt caactgcacc aacagataga gcttcacaac gttcaatcac
     3061 aaaagaacgt cagtctactg ttcaaagagt accactacaa aatacaaaaa gtagaccacc
     3121 aatcaaaacc gccaccatta agaaaggcag taagaaacca tgaagctgaa aactttagtg
     3181 attggtggtt cgggattatt cttgatggtc ttctcactgc ttctgtttgt cgccatttta
     3241 ttttcagatg aacaagatgg cggtttttcc aacattcact atggcggtgt ggatgtttcc
     3301 gcagaagtgc tggctcataa gcctatggta gaaaaagttg ccaaagaata tggaattgaa
     3361 gaatacgtca atatacttct tgcgattata caggtggaat cgggtggtac agcagaagat
     3421 gttatgcagt cctcggaatc cctcggtctt ccacctaatt cattgagtac agaagaatcc
     3481 attaagcaag gtgtgaagta tttcagtgag ttattagcca gtagcgaaag gcttggtgta
     3541 gatttagaat cggttatcca gtcctacaat tatggtggtg gtttcttagg atatgtggct
     3601 aatcgtggga ataaatatac ctttgaactg gctcaaagtt tctcaaaaga gtattcgggt
     3661 gacgaaaaag tgtcttaccc caatcccata gccataccta tcaatggggg ctggcgatac
     3721 aattacggga atatgtttta tgtgcaactg gtaacgcagt atcttgtcac aacagagttt
     3781 gatgatgata cagtacaagc catcatggac gaagctctga aatttgaagg ctggaagtat
     3841 gtctatggcg gagcttcccc gactacctct tttgattgta gcggactgac acaatggacg
     3901 tatggaaaag ctggaatcaa cttaccacga acagcacaac agcaatatga tgtaacccgg
     3961 catatcccat tattggaagc aaaagccggc gatttggttt tctttcattc tacctataac
     4021 gctggctctt atattactca tatcgggatt tatttaggcg ataaccgtat gtttcatgca
     4081 ggcgacccta tcggttatgc cgacttaaca agctcttact ggcaacagca tttagtggga
     4141 gcaggacgaa tcaaacaata agaaaggacg attcaatgat gaaattcgga aaaaatcaga
     4201 atacagaaaa acagacacca aaggaaaaga aacctcgtgt ctataaggtc aatcctcgta
     4261 aaaaggttgt gattgtccta tgggtacttt taggacttag tttcagcttt gcgatattca
     4321 agcactttac agccattgat acccatacca ttcatgaaac caccatcata gaaaaggaat
     4381 atgtcgatac tcatcatgtg gaaaattttg tagagaactt tgcgaaagtc tactattcat
     4441 gggaactaaa cgacaagtcc attgataatc gcatagaaaa cctgaaagca tatctgacag
     4501 aggaacttca agctctgaat gtcgatacag ttcgtaaaga tattcctgta tcgtcttctg
     4561 taaaaggatt tcagatatgg acggtagaag cagatggcga caatcaattt gatgtaacct
     4621 acagtgtaga ccaactcatt acagaggggg aaaatacaaa gaccgtccac tctgcttata
     4681 tagtgagtgt ctatgtagac agtactggaa atatggtcct cattaagaat ccgaccatta
     4741 ctagcatacc aaagaaatca gactataaac caaaagctat tgaaagtgag ggtacggttg
     4801 attccattac aaccaatgaa atcaatgagt ttttaacgac gttcttcaag ctctatccta
     4861 cagcgacagc cagtgaactt tcctactatg tgaatgacgg gatattaaaa ccaatcggaa
     4921 aagagtacat ctttcaagaa ctggtaaatc ctatttacaa tcgtaaggat aatcaagtca
     4981 cggtatcgct gacagtggag tatatcgacc agcagaccaa agcaacgcag gtatctcaat
     5041 ttgatttggt acttgaaaag aacgggagta attggaagat tatagaataa caaatattgg
     5101 tacattatta cagctatttt gtaatcacgt actctctttg ataaaaaatt ggagattcct
     5161 ttacaaatat gctcttacgt gctattattt aagtatctat ttaaaaggag ttaataaata
     5221 tgcggcaagg tattcttaaa taaactgtca atttgatagt gggaacaaat aattggatgt
     5281 ccttttttag gagggcttag ttttttgcac ccagtttaag aataccttta tcatgtgatt
     5341 ctaaagtatc cggagaatat ctgtatgctt tgtatgccta tggttatgca taaaaatccc
     5401 agtgataaga gtatttatca ctgggatttt tatgcccttt tgggcttttg aatggaggaa
     5461 aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcggg aaaaactacc
     5521 ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag cgtggacaaa
     5581 ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac aattcagaca
     5641 ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac gccaggacat
     5701 atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc aattctactg
     5761 atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc acttaggaaa
     5821 atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat tgatttatca
     5881 acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa acagaaggta
     5941 gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg ggatacggta
     6001 atagagggaa acgatgacct tttagagaaa tatatgtccg gtaaatcatt agaagcattg
     6061 gaactcgaac aagaggaaag cataagattt cagaattgct ccttgtaccc tgtttatcat
     6121 ggaagcgcaa aaagcaacat agggattgag cagcttatag aagtgataac gaataaattt
     6181 tattcatcaa catacagaaa gaagtctgaa ctttgcggaa atgtcttcaa aattgaatat
     6241 tcggaagaaa gacaacgtct tgcatatgta cgcctttatg gcggaatcct gcatttgcgg
     6301 gattcggtta gaatatcgga aaaggaaaaa ataaaaatta cagaaatgta tacttcaata
     6361 aatggtgaat tatgtaaaat tgataaggct tattccgggg aaattgttat tttgcaaaat
     6421 gagtttttga agctaaatag tgttcttgga gatacaaagc tattgccaca gagagagaga
     6481 attgaaaatc cgctccctct gctgcaaaca actgttgaac cgagcaaacc tcaacaaagg
     6541 gaaatgttac ttgatgcact tttagaaatc tccgacagtg acccgcttct acaatattat
     6601 gtggattcta cgacacatga aattatactt tctttcttag ggaaagtaca aagggaagtg
     6661 attagtgcac tgttgcaaga aaagtatcat gtggagatag aactaaaaga gcctacagtc
     6721 atttatatgg agagaccgtt aaaaaatgca gaatatacca ttcacatcga agtgccgcca
     6781 aatcctttct gggcttccat tggtttatct gtatcaccgc ttccgttggg aagtggaatg
     6841 cagtatgaga gctcggtttc tcttggatac ttaaatcaat catttcaaaa tgcagttatg
     6901 gaagggatac gctatggttg cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa
     6961 atctgtttta agtatggctt atactatagc cctgttagta ccccagcaga ttttcggatg
     7021 cttgctccta ttgtattgga acaagtctta aaaaaagctg gaacagaatt gttagagcca
     7081 tatcttagtt ttaaaattta tgcgccacag gaatatcttt cacgagcata caacgatgct
     7141 cctaaatatt gtgcgaacat cgtagacact caattgaaaa ataatgaggt cattcttagt
     7201 ggagaaatcc ctgctcggtg tattcaagaa tatcgtagtg atttaacttt ctttacaaat
     7261 ggacgtagtg tttgtttaac agagttaaaa gggtaccatg ttactaccgg tgaacctgtt
     7321 tgccagcccc gtcgtccaaa tagtcggata gataaagtac gatatatgtt caataaaata
     7381 acttagtgta ttttatgttg ttatataaat atggtttctt gttaaataag atgaaatatt
     7441 ttttaataaa gatttgaatt aaagtgtaaa ggaggaatta attatgaagt gtaatgaatg
     7501 taacagggtt aaatacaacc aaattttaat ttggctttgc gttttatctt tttttagcgt
     7561 attaaatgaa atggttttga acgtctcatt acctgatatt gcaaatgatt ttaataaacc
     7621 acctgcgagt acaaactggg tgaacacagc ctttatgtta accttttcca ttggaacagc
     7681 tgtatatgga aagctatctg atcaattagg catcaaaagg ttactcctat ttggaattat
     7741 aataaattgt ttcgggtcgg taattgggtt tgttggccat tctttctttt ccttacttat
     7801 tatggctcgt tttattcaag gggctggtgc agctgcattt ccagcactcg taatggttgt
     7861 agttgcgcgc tatattccaa aggaaaatag gggtaaagca tttggtctta ttggatcgat
     7921 agtagccatg ggagaaggag tcggtccagc gattggtgga atgatagccc attatattca
     7981 ttggtcctat cttctactca ttcctatgat aacaattatc actgttccgt ttcttatgaa
     8041 attattaaag aaagaagtaa ggataaaagg tcattttgat atcaaaggaa ttatactaat
     8101 gtctgtaggc attgtatttt ttatgttgtt tacaacatca tatagcattt cttttcttat
     8161 cgttagcgtg ctgtcattcc tgatatttgt aaaacatatc aggaaagtaa cagatccttt
     8221 tgttgatccc ggattaggga aaaatatacc ttttatgatt ggagttcttt gtgggggaat
     8281 tatatttgga acagtagcag ggtttgtctc tatggttcct tatatgatga aagatgttca
     8341 ccagctaagt actgccgaaa tcggaagtgt aattattttc cctggaacaa tgagtgtcat
     8401 tattttcggc tacattggtg ggatacttgt tgatagaaga ggtcctttat acgtgttaaa
     8461 catcggagtt acatttcttt ctgttagctt tttaactgct tcctttcttt tagaaacaac
     8521 atcatggttc atgacaatta taatcgtatt tgttttaggt gggctttcgt tcaccaaaac
     8581 agttatatca acaattgttt caagtagctt gaaacagcag gaagctggtg ctggaatgag
     8641 tttgcttaac tttaccagct ttttatcaga gggaacaggt attgcaattg taggtggttt
     8701 attatccata cccttacttg atcaaaggtt gttacctatg gaagttgatc agtcaactta
     8761 tctgtatagt aatttgttat tacttttttc aggaatcatt gtcattagtt ggctggttac
     8821 cttgaatgta tataaacatt ctcaaaggga tttctaaatc gttaagggat caactttggg
     8881 agagagttca aaattgatcc tttttttata acaggaattc aaatcttttt gttccattaa
     8941 agggcgcgat tgctgaataa aagatacgag agacctctct tgtatctttt ttattttgag
     9001 tggttttgtc cgttacacta gaaaaccgaa agacaataaa aattttattc ttgctgagtc
     9061 tggctttcgg taagctagac aaaacggaca aaataaaaat tggcaagggt ttaaaggtgg
     9121 agattttttg agtgatcttc tcaaaaaata ctacctgtcc cttgctgatt tttaaacgag
     9181 cacgagagca aaacccccct ttgctgaggt ggcagagggc aggttttttt gtttcttttt
     9241 tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt tggtcggcac tgccgacagc
     9301 ctcgcagagc acacacttta tgaatataaa gtatagtgtg ttatacttta cttggaagtg
     9361 gttgccggaa agagcgaaaa tgcctcacat ttgtgccacc taaaaaggag cgatttacat
     9421 atgagttatg cagtttgtag aatgcaaaaa gtgaaatcag ctggactaaa aggcatgcaa
     9481 tttcataatc aaagagagcg aaaaagtaga acgaatgatg atattgacca tgagcgaaca
     9541 cgtgaaaatt atgatttgaa aaatgataaa aatattgatt acaacgaacg tgtcaaagaa
     9601 attattgaat cacaaaaaac aggtacaaga aaaacgagga aagatgctgt tcttgtaaat
     9661 gagttgctag taacatctga ccgagatttt tttgagcaac tggatccagg agaacaaaaa
     9721 cgattttttg aggaaagtta taaattattt tccgaacgat atggcaagca aaatattgct
     9781 tatgcaacag ttcataatga tgagcaaacc cctcacatgc atttaggtgt tgtgcctatg
     9841 cgtgatggaa aactgcaagg aaaaaatgtg tttaatcgtc aagaactgtt atggctacaa
     9901 gataaattcc ccgagcatat gaaaaaacag ggttttgagt tgaagcgtgg tgaacgtggc
     9961 tctgaccgta aacatattga gacagctaaa tttaaaaaac aaactttgga aaaagagatt
    10021 gattttctag aaaaaaattt agcagttaaa aaagatgaat ggactgctta tagcgataaa
    10081 gttaaatcag atttagaagt accagcgaaa cgacacatga aaagtgttga agtgccaacg
    10141 ggtgaaaagt ccatgtttgg tttgggaaaa gaaataatga aaacagaaaa gaaaccaacc
    10201 aaaaatgttg ttatatcgga gcgtgattat aaaaacttag tgactgctgc gagagataac
    10261 gataggttaa aacagcatgt tagaaatctc atgagtactg atatggcgag agaatataaa
    10321 aaattaagta aagaacatgg gcaagttaaa gaaaaatata gtggtcttgt agagcgattt
    10381 aatgaaaatg taaatgatta taatgagttg cttgaagaaa acaagtcttt aaagtctaaa
    10441 ataagcgatt taaagcgtga tgtgagttta atctatgaaa gcactaagga attccttaag
    10501 gaacgtacag acggcttaaa agcctttaaa aacgttttta aggggtttgt agacaaggta
    10561 aaggataaaa cagcacaatt ccaagaaaaa cacgatttag aacctaaaaa gaacgaattt
    10621 gaactaactc ataaccgaga ggtaaaaaaa gaacgaagtc gagatcaggg aatgagttta
    10681 taaaataaaa aaagcacctg aaaaggtgtc tttttttgat ggttttgaac ttgttctttc
    10741 ttatcttgat atatatagaa ataacgtcat ttttatttta gttgctgaaa ggtgcgttga
    10801 agtgttggta tgtatgtgtt ttaaagtatt gaaaaccctt aaaattggtt gcacagaaaa
    10861 accccatctg ttaaagttat aagtgaccaa acaaataact aaatagatgg gggtttcttt
    10921 taatattatg tgtcctaata gtagcattta ttcagatgaa aaatcaaggg ttttagtgga
    10981 caagacaaaa agtggaaaag tgagaccatg gagagaaaag aaaatcgcta atgttgatta
    11041 ctttgaactt ctgcatattc ttgaatttaa aaaggctgaa agagtaaaag attgtgctga
    11101 aatattagag tataaacaaa atcgtgaaac aggcgaaaga aagttgtatc gagtgtggtt
    11161 ttgtaaatcc aggctttgtc caatgtgcaa ctggaggaga gcaatgaaac atggcattca
    11221 gtcacaaaag gttgttgctg aagttattaa acaaaagcca acagttcgtt ggttgtttct
    11281 cacattaaca gttaaaaatg tttatgatgg cgaagaatta aataagagtt tgtcagatat
    11341 ggctcaagga tttcgccgaa tgatgcaata taaaaaaatt aataaaaatg gttctgttgc
    11401 aaagttttaa ataaagaata aa
//
LOCUS       DACEQH010000241         7959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.guided.090, whole genome shotgun sequence.
ACCESSION   DACEQH010000241 DACEQH010000000
VERSION     DACEQH010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.guided.090"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            128..1564
                     /locus_tag="IW833_002913"
     CDS             128..1564
                     /locus_tag="IW833_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1308933.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="IW833_002914"
     CDS             1753..4200
                     /locus_tag="IW833_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1308934.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="IW833_002915"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="IW833_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1308935.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="IW833_002916"
     CDS             complement(5690..6793)
                     /locus_tag="IW833_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1308936.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="IW833_002917"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="IW833_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1308937.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1359 c         1729 g         2206 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DACEQH010000242         7482 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.guided.094, whole genome shotgun sequence.
ACCESSION   DACEQH010000242 DACEQH010000000
VERSION     DACEQH010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.guided.094"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            109..192
                     /locus_tag="IW833_002918"
     CDS             109..192
                     /locus_tag="IW833_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1308938.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="IW833_002919"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="IW833_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1308939.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1224..1484
                     /locus_tag="IW833_002920"
                     /pseudo
     CDS             1224..1484
                     /locus_tag="IW833_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1647..2312
                     /locus_tag="IW833_002921"
                     /pseudo
     CDS             1647..2312
                     /locus_tag="IW833_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2348..2593
                     /locus_tag="IW833_002922"
     CDS             2348..2593
                     /locus_tag="IW833_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1308940.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2696..3565
                     /locus_tag="IW833_002923"
     CDS             2696..3565
                     /locus_tag="IW833_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1308941.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3546..4280
                     /locus_tag="IW833_002924"
     CDS             3546..4280
                     /locus_tag="IW833_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1308942.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4313..5225
                     /gene="ant(6)"
                     /locus_tag="IW833_002925"
                     /pseudo
     CDS             4313..5225
                     /gene="ant(6)"
                     /locus_tag="IW833_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5222..5764
                     /gene="sat4"
                     /locus_tag="IW833_002926"
     CDS             5222..5764
                     /gene="sat4"
                     /locus_tag="IW833_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1308943.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5857..6651
                     /locus_tag="IW833_002927"
                     /note="aph(3')-IIIa"
     CDS             5857..6651
                     /locus_tag="IW833_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1308944.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6759..6947)
                     /locus_tag="IW833_002928"
                     /pseudo
     CDS             complement(6759..6947)
                     /locus_tag="IW833_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6927..7469
                     /locus_tag="IW833_002929"
     CDS             6927..7469
                     /locus_tag="IW833_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1308945.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT         2623 a         1131 c         1567 g         2161 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt ttgtgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaataa
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
     1081 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     1141 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     1201 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg
     1261 tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg
     1321 aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag
     1381 aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa
     1441 ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacatgg ggctgaccca
     1501 aaagtagctc ctatatgaaa aatagaggtc tttccattta gatagtagtg gaaagacctc
     1561 tatttatctt ttgaaagtga atacaaaaat aaagcacact ctgctataat ataggtgatt
     1621 aatccatttg agagaggtgc tttatcatgt atcaaaatta taccataaac caactttgtt
     1681 tgcctataga tcttgaaatt aaattagaag aaaatgattt tgctcatgcc attgtgcagt
     1741 ttgttgactc tattcccgat gaagtctttc ttccttatta tcaatctatg ggtagacccc
     1801 aatatcatcc tcgtatgcta ctttccatta ttctttgtgc ctatattcaa ggggtttatt
     1861 cgggccgcca aatacaaaat atgctgatag atagtattcg gatgcgctac ttatctcaag
     1921 agcaattccc taactttcgt actattaatc gttttcgcgt tcatccgatt atgaacgata
     1981 ttctagatca ttcatttgtt caatttcgtg agctactggt tcaaagtggc ttaatcagtg
     2041 gcagtgctct atacattgat ggtacaaaaa ttgaagcgga tgcgaataaa tactctttcg
     2101 tttggaaaaa aagtatttta aagtataagg atagccttga tcaaaaagcc cttgaaaact
     2161 accaacaaat ggtggaaaca aaaatcttac ctgagttaat agatgattta aaggatgaac
     2221 tttctataga agagattgaa aggattcgtc aatctttgga agaaaaggaa aatcaattaa
     2281 ttgaagcgat tgaccaaacc gaaacagtag aagggggctg acccattatt aataaggagt
     2341 ggacaacatg attgatgtaa atgagctatt ggaagaagct attagagaaa ctgaaaatct
     2401 aaatgatggc gaagtttttc ttgttaaaga tttattcaag ggatatgtgg ggaataggat
     2461 acccagaaag gatcgacttc tgcttggaac gttattttta aaccatgtta gcaaaatgaa
     2521 tggtaattta aaggctattg aaaagacttc atctaatcag cagaggtata aaaagacaat
     2581 tgataagttt taactatctt ttgtgaaatt aaaaaaatta taaatatgtt gagtagaagc
     2641 aaaagatttg gcttggaatt tttacagcag tgaatcgata aaaggagcat gcttgatggt
     2701 agataatatt attaaatcag tagcagagaa attatcctct ctgtcttata tagaaggtat
     2761 tgttttaggt ggttcacgtg caaggggcac ccatacagag gattcggata tagatatcgg
     2821 catctattac aattcagaat catttgacat aaatactatt aatcaattcg ctacaaagct
     2881 ggatgatgag catagaaata accttgttgt acctcccgga gcatggggtg attggattaa
     2941 tggcggcgga tggttagtca taaacgggta tcatgtggat ttaattttac gtgatattaa
     3001 acgtgtggaa caaataatga aagatacaga gcacggaatt gttactgcca attatcagac
     3061 tgggcatccc catggttata ttagtgcaat gtatcgagga gaattagcga ttagcaaaat
     3121 actatatgct aagaatgaaa gcttatgcga attaaaaaaa caggcagaaa cttatcccaa
     3181 tgctttgcag aaaagtttag ttaacttttt tatgtttgaa gcagggttct ctttaatgtt
     3241 tgtaaaagca aattcgggaa cagacgataa atattatatt gcgggtcatg tttttcgtat
     3301 agtttcatgt ttaaatcaag tgttatttgc atgtaataat gcttattgta tcaacgaaaa
     3361 gaaagctata aaactgcttg aaacttttga acataaacct gaaaaatata ccgagaaggt
     3421 aaatcatatt tttgaagtac tcggtatctc actttttgaa tgctacgaca tgaccgagaa
     3481 gctttataat gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag
     3541 ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga
     3601 gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac
     3661 ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat
     3721 atgcgatgga aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg
     3781 aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag
     3841 atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg
     3901 tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt
     3961 tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata
     4021 acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga
     4081 cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa
     4141 atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga
     4201 acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat
     4261 cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc
     4321 agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac gaacgtattc
     4381 gaattgtgac ccttgagggg tcacgcgcaa atattaatat acctaaagat gaatttcagg
     4441 attatgatat tacatatttt gtaagtgata tagaaccgtt tatatctaat gatgactggc
     4501 ttaatcaatt tgggaatata ataatgatgc aaaagccgga ggatatggaa ttattcccac
     4561 ctgaagaaaa gggattttcc tatcttatgc tatttgatga ttacaataaa attgatctta
     4621 ccttattgcc cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc
     4681 taattgataa agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg
     4741 taagaaagcc aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac
     4801 cttatgttat taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc
     4861 agattgttcg ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct
     4921 ttaaattaag tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt
     4981 gggagaaact tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat
     5041 ttctatgcca tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg
     5101 cctatccgga gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca
     5161 ctggtaaaac cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac
     5221 agtgattaca gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt
     5281 tgaggtgata ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt
     5341 actctatgaa gcgccatatt taaaaagcta ccaagacgaa gaggatgaag aggatgagga
     5401 ggcagattgc cttgaatata ttgacaatac tgataagata atatatcttt actaccaaga
     5461 cgataaatgc gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg cttatataga
     5521 agatatcgcc gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat
     5581 atctatagaa tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa
     5641 taaccttata gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac
     5701 tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt atttaaggtt
     5761 ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat
     5821 ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg
     5881 gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct
     5941 gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc
     6001 cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa
     6061 ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg
     6121 ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct
     6181 gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg
     6241 gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat
     6301 aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg
     6361 cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac
     6421 ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat
     6481 cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc
     6541 agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag
     6601 cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta gtacctagat
     6661 ttagatgtct aaaaagcttt aactacaagc tttttagaca tctaatcttt tctgaagtac
     6721 atccgcaact gtccatactc tgatgtttta tatcttttct aaaagttcgc tagatagagt
     6781 tctatattag agatgtaaag agttttggtg aagaggtttg tgagtatggc ttttataacc
     6841 agggtgcaat gagaagcact aacagcagct agcttaagaa cgcataggat atacttttta
     6901 tggaagtcac agaggaggaa taaaatttga acaggattaa cagagtaacc tctattctta
     6961 ttcagctgca atcaaaaaaa ataattcctg ccaaagaaat tgcacagcga tttaatataa
     7021 gcttaaggac agtttacaga gatatccgga cacttgaaga agccggaata ccaattggat
     7081 ctgaggccgg aaaaggatat tttcttgtag agggcttcct acttccgccg gtaatgttta
     7141 cagcggcgga agtgggtgca ttgattacag cagggaaatt tttaaattgc catggagatg
     7201 aatcatttat aaaggatttt gattcagcta tgtataaaat caaatctatt ttgaagcatg
     7261 gtgaaaaaaa ctatgcacag gagttggaga acagtattaa cgtgtacagc acatctggac
     7321 agaaaaatac attggcggat aacgttattg cagcaattca gactgcaatc tgcaataaaa
     7381 gggtaatatc aattcaatat cctgcatcag gagggcaaga accggaaagc agaatgatag
     7441 agccggttct gttgcaaagt tttaaataaa gaataaaatc cc
//
LOCUS       DACEQH010000243         6950 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.guided.100, whole genome shotgun sequence.
ACCESSION   DACEQH010000243 DACEQH010000000
VERSION     DACEQH010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.guided.100"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            289..564
                     /locus_tag="IW833_002930"
     CDS             289..564
                     /locus_tag="IW833_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1308946.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            577..2655
                     /locus_tag="IW833_002931"
     CDS             577..2655
                     /locus_tag="IW833_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1308947.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2815..4701
                     /locus_tag="IW833_002932"
     CDS             2815..4701
                     /locus_tag="IW833_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1308948.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4713..5660
                     /locus_tag="IW833_002933"
     CDS             4713..5660
                     /locus_tag="IW833_002933"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1308949.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5680..6207
                     /locus_tag="IW833_002934"
     CDS             5680..6207
                     /locus_tag="IW833_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1308950.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6270..6923)
                     /locus_tag="IW833_002935"
     CDS             complement(6270..6923)
                     /locus_tag="IW833_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1308951.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
BASE COUNT         2338 a         1173 c         1474 g         1965 t
ORIGIN      
        1 tgattcatca gtcccataaa ttatagagcc gatttttgtc gcagccccat ttttttgttg
       61 tgataaaaga acaagaaagg caggaatggg aaagtaaaca tccttttgtc agaaactttt
      121 atctttctta agttttactt gatatctgat actatttgga atagaatgag gcgttgaaaa
      181 atttatggaa atttctaact agcaataaac gatgaaacaa tgaaatattt ttcatccaaa
      241 aagaactttg ctataatgat ggaagtttgt gtataggaga tctggaaaat gagagagagt
      301 tttgaacaac aaaaaaaact gttgcatgat cgctatggcg ccctttcgat ggatgaccgc
      361 cgtcaaatac tttgtaagtt gcgtaaaaga aatatattga tgtatcgtca gttggaacgt
      421 ctgaaacatg atctgcttcg tttggagtca aaacgtgtac agtgtgaatt agaggggaac
      481 caaacacagg ttgaagtagt cgaaaccaaa attttaaaga aaaaagaaca atttttaaaa
      541 atgcttacac aaaataaaaa ataaagggga tggacgatgg aatttgctga gttagttatt
      601 gtatttgcag taacgatcac cttatcgaac atagctagcc gcattttgcc aatgattcca
      661 gctccactga tccaaatttt ccttggagtg gtattaggtc tgacagagtg gggacaatca
      721 atcgattttg aacctgaatt gttcttagtt atgattattg caccattgtt atttagagaa
      781 ggagaaaaag cagatatttc ttcgattctc aaaaatttcg ggacaatttt gttcttggca
      841 tttggaggag tcattttgac ccttgtcggg gtaggtgcga cgttatcatt cctgttgcca
      901 agtgtcccac ttgctgcctg ctttgctttt ggagcagcgt taggtccgac agatgcggta
      961 gcagtcagtt ctctttctgg acgagtgaat atccctaaaa aggcgatgca tattttggaa
     1021 ggagaaggtc ttttgaatga tgcatctggt gttactgcct tccagtttgc tcttggtgcg
     1081 ctgatcaccg gatctttttc agcagtaaac gcggggatga gtctagtagt ttctagtatt
     1141 gggggagctt tgatcggctt tttgcttgtc tggttcaaac aaaaaataat tcatttgatc
     1201 gaaaaagcat cagcacaaga tgtcacaggg tatctgctga tcgaactttt gcttcctttt
     1261 ctagcctatg tattggcaga ggcatttgat gtatctggaa tcattgctgc agtggcagca
     1321 ggtattctcc aagcttctgg ttttaggaaa atctctgtgt ttgatgcaga attgtcgagc
     1381 ttgtcccaca gtacatggac aactattgct tttacactga atgcattagt atttatattt
     1441 ttaggaatcg aattgacgca agtgttctcg ccagtatggg gagacggttt atatccaaac
     1501 ggtcttttat tagcgattat cgtattaatc tctgtcatgc tttttgtgat cagattcatt
     1561 tctatcagtc ttttttatgt attcaaagat ggcagtaaaa agttcaaaaa acagttgaac
     1621 gagatcttga tcttaacatt tggcggtgta aagggaacag tcagtttggc tacgattttt
     1681 attttgccat tttcaatcaa taatatgatg ttttaccagc ggtctttgct tttgtttctg
     1741 acggcgggtg tcatcttagt gactttggtc atagggatca ttgtcttgcc tatgctgacg
     1801 gaaacagagg aagcgaaaag cacggatttg aatgctttga tgattttaga agaggtcgtt
     1861 gaagttttgc gaaaagaaat aaaggaaatt gatcgtaata caaaagaatt tttagccact
     1921 gaagcagtga tagaaaatta tcaagaacgg atacgtgatc tgtacttgga agatttaacc
     1981 gatgatgaaa agcaagaagt acaagaaata caagcactga ttttgtcgat cgaacgggat
     2041 ggattggatg aaagttatcg ttcgggcaag ctttcttcta atggttatcg tttttactcc
     2101 cgttttcttt ctcgattcga acattctatc acaagccaga ttttgtcttt tatcggcttt
     2161 tggttcattg ttgtccgtag gttaatgcga atcgttctcc atccgaaaat gttttggcaa
     2221 agaagatatg ctgatcgtca gacatttatt agtgatgaag atatccaaga aatcagagag
     2281 acatatcaaa aaaatactca gttgattatt gaaagtttag ataacttgac tgatatttat
     2341 gatgaaacga tggttcaatt ttttatccag caacgcaaac ttgaaggaat aaaaatgaac
     2401 tcggggaatt tgatttcttc gtggatgatc cagcaagata gtctgtttac gaaaaaaatg
     2461 ctaagaggtt attatcttga aagaaaaatc attgatgaat acgaagttgc tgaaaaaatc
     2521 acgacgtttt ctgcgaacag ctatcgcagg aatatcaatt tgttagaatc ttatacgatg
     2581 aacaaaccat ctgataactt ttctttccga tttgcgtttc gagcaaagtc aaaaaaaacg
     2641 agtccaataa aataaaaagg taaaaatttt caagttttat aaaaaacaaa acatatcaga
     2701 acaggatcga atcggtcctg ttcttttttt gagaacacaa aaagaacaaa taccaaaaaa
     2761 attttcctga agttcactat ttttatgata caatatccaa gataaggagg gaacatgttg
     2821 aaagaattaa aagtaactga cctcaaaaaa acttatggtg aaaaagattt gtttgaccag
     2881 atttcttttt tggtccacga aaaagaccgt atcgggttga ttgggacgaa cgggacgggt
     2941 aaaacaagtc tcttaaatat tattgccggt atcgatagca gcgacggtga tcggcaaaca
     3001 gtgttttatc caactgatta ccgtattggt tatctttctc aagcagctga attttccgat
     3061 gaattaactg ttctccaagc agtctttcaa ggaaacagcc ccttgatcca aactgttcga
     3121 gcatatgagg aagcattgat cgaactaggg gaaaacggag aagaccctga tgtgcaaaaa
     3181 cgttatgcta aagcagaaga acaaatgaat aaagaagatg cttggactac agataccaac
     3241 gcaaagatta ttttgcaaaa attaggaatc agtgaattag ataaaaaaat cagtacttta
     3301 tcaggtggtc agaaaaaacg agttagtctg gcgcaagtgt tgattgatga acccgattta
     3361 ctattactag acgaaccaac aaaccattta gattatgaag cgatcgagtg gttggaaaac
     3421 tatttgaaac aataccgtgg cgcactattg atggttaccc acgatcgtta ttttctagat
     3481 agagtagcaa accgaatctt cgaactttca tttggaaaat tatacgagta taaaggaaat
     3541 tatgaagctt acgtcttaga aaaagcagaa cgtgatcgtg tagaagtaga acaagaagaa
     3601 aagcgcaagc ggctttataa acaggaatta gcttggatga gggctggcgt acaggcacgt
     3661 ggcaccaagc agcaagcacg gatcaaccgt ttcgaagatc taaaggagaa cttgtaccaa
     3721 gtaaaccaag aagatgattt agagctgaac ctagcgacac aacgtttagg gaaaaaagta
     3781 ctggaaatca aagatggcag ttaccgtata aatgaccaaa cactgctaga acatttagat
     3841 ttattgattc aatctcggga aagactaggt attactggta aaaatggtgc gggaaaatcg
     3901 acattgctta atattctagc tggcagaatt cccctggata gcggtacaat gtctatcggt
     3961 gaaacggttc atcttgcgta ttatacacaa gaaaatgaag aaatggcacc agataaacgg
     4021 atgattgctt acttacaaga agctgctgaa gaagcaaaaa cagcagatgg cagtcaaatc
     4081 ggagtagcag aattacttga acgattttta tttccaagat ttatgcatgg gacactgata
     4141 ggcaaacttt caggcggaga aaaacgccgg ttatttttgt tgaaactgct gattcagcaa
     4201 cctaatgttt tattattgga tgaaccgacc aatgatttgg acatagcaac attgacgatt
     4261 ttggaagatt attttagaag ttttccaggt gctgtgatca ctgtttccca tgaccgttat
     4321 ttcttggaca aggtagcaga taagttatta gtattccaag gaaacggaaa acaagaactt
     4381 tattacggaa acatgtccag ttatttactg aagcaaaaag agacccagca gcctgctgaa
     4441 aaagcgaaac caaaaataca gagcaaggaa cctgctgaaa agaagaaatt atcttacatg
     4501 gaacaaaaag aatgggaaac gattgaagat gagattgcag aacttgaaga aaaaatctca
     4561 ttgctccaag aagagatgaa tcatcaagga gataatttca ctcgattaca agaattgcaa
     4621 aacgacgtat ctgaaacaga agcacaactt gaagaaaaaa tggcacggtg ggaatacttg
     4681 agtgaatggg tagaagatta ggaggaaaaa ggatggaaca agcgtatctt gacttaggaa
     4741 gaaaattgtt ggaagaaggg catcgtaaag aagacagaac aggcacaggc acattaagct
     4801 tattcggata ccaaatgcgg tttgatctgc aaaaagggtt tcctttgtta acaacgaaac
     4861 gagtaccttt tggattgatc aaaagcgaat tgctttggtt tttaaaagga gatacgaata
     4921 tccgctatct attacaaaat aaaaaccata tttgggatga atgggcattt gaacgctaca
     4981 tcaaaagtcc agactataat ggaccagata tgactgattt tggccataga tgtttgacag
     5041 atcctgaatt caatgaagaa tataaaaagc agagcaagct tttttgcgag aagatcctaa
     5101 ccgatgattc ctttgctgaa acatacggcg atcttggaca catctacggc tatcaatggc
     5161 gtcactggga aacaaaagac ggtggcttta tcgatcaaat caaacaagtg atcgaagcaa
     5221 tcaaaaagac tcctgattct agaagattga tcgtttctgc atggaatccg gaagatgttc
     5281 ctagcatggc tttgcctcct tgtcatacga tgtttcaatt ttatgttcaa gagggacggc
     5341 taagctgcca attgtatcaa cgcagtgggg atgtgtttct aggcgttccg ttcaacattg
     5401 caagttatgc gttgctgact cacctaattg cacatgaaac aggtttagcg gttggcgaat
     5461 ttgttcacac attaggagat gctcatcttt atatcaatca tatcgaacaa atgaaagaac
     5521 aattgagccg agaaatacgc gcatttccga ctttagtttt gaatcaggaa aagcagtcag
     5581 tatttgattt tgagatggaa gatatccaat tagaaggata tgacccacat ccggcgatca
     5641 aagctcctat cgctgtttag aatcagaaag gatgagaaaa tgttagctgc aatttgggca
     5701 caggatgaaa acggattgat tggaaaagaa gaccgtcttc cttggcgttt gccaaatgat
     5761 ctgaaattct tcaaacaaat gacagaagca aatacactag tgatgggacg aaaaacattc
     5821 gaagggatgg gtagccgtcc gttgccgaat cgtcagacga ttgttttgac gagagattct
     5881 tcctataaag cagaaggcgt gcatgtcatg catagtgtgg aagacgtgct tgctctggaa
     5941 aaagaaacag acggtatctt tttcatcgct ggagggtcag ctgtttatca agaatttctg
     6001 ccattttgta cgatattata ccgtacagtc atccatcatg tattcgatgg agatgcttat
     6061 tttccacctg ttgactggtc tgattggtca ctgatcaata ctagtcaagg agaaatggat
     6121 gaaaaaaatc gttatccgca tcaatttgaa acgtatcagc gtaatgagaa tcctgtaaaa
     6181 agcgggacag gcgaattgac gatataaaaa agctatggat agactagctg ttatttagag
     6241 ctgtcccatc catagcttta ttttttttat taagtaaaga ataaaacaga gaagtacata
     6301 aaacctgcac ctaacataac aaacaaatgc caaacaacat gcatatagcg gatagaattc
     6361 aagctataaa agaatgcgcc tatcgtgtaa gaaagcccgc ctgctaccat caaaataaca
     6421 ccagtcattc caagagagtg ataaagagga acagcagcca cgacacagag ccaccccata
     6481 aaaatataga tgatcgtaga tacttttgat actctctccc gtttatgcaa cgtcaacgct
     6541 ttatatataa tgccaagaat cgcaaacaac cagattaata caaataaagt ccaacctagc
     6601 catcctcgga tactcagcaa acaaaaagga gtataacttc ctgcaatcaa aagaaaaatc
     6661 gaactatgat cgaatacttg gaacaccttt tttgcttttg taaaaatcaa tgaatgaaac
     6721 aaagtagacg ttaaaaataa aagaatcatc atcgatccat agatagcata agagacaata
     6781 tggagggagg aatgcaagtg ggcacctttt actaaaagaa ccactagtcc cgcgatactc
     6841 aatccagtcc caattccatg ggtgactgca ttaaacactt catttaatat ttcatacgtt
     6901 cgattaaatt tcgttttttc catgaaaacg gctcctttac taggtgtaat
//
LOCUS       DACEQH010000244         4995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.guided.110, whole genome shotgun sequence.
ACCESSION   DACEQH010000244 DACEQH010000000
VERSION     DACEQH010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.guided.110"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(30..2180)
                     /locus_tag="IW833_002936"
                     /pseudo
     CDS             complement(30..2180)
                     /locus_tag="IW833_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2326..2901
                     /locus_tag="IW833_002937"
     CDS             2326..2901
                     /locus_tag="IW833_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1308952.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3115..3810
                     /gene="vanR"
                     /locus_tag="IW833_002938"
     CDS             3115..3810
                     /gene="vanR"
                     /locus_tag="IW833_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1308953.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3788..4942
                     /locus_tag="IW833_002939"
     CDS             3788..4942
                     /locus_tag="IW833_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1308954.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1560 a          939 c          967 g         1529 t
ORIGIN      
        1 aattttcttc tttcagcttt ttatttccca cttgtaaatc ggtagatggt ggctcctttt
       61 ccagttccat aatgtggatt tgcatctgca tgtagtgatg aaacacctag ctgcattctc
      121 ataccatctg acgaagatgt tgtaccgtcg ccccaataga aaggcaattg taatttatga
      181 tgaaagttta ctaatatggc ttgggcttta ttcatggcat cttcatacat gcgccattga
      241 gatacattgg ctagttgctt atatgtaagt ccgggtgtgg cttcggccat cttgctcaag
      301 ccaatattca ttcccattcc taaaagggca gccatgataa tgattgtttc ttccttatct
      361 ggttttcgat tattggaagc atgagtgaat tgctcatgaa atcctgttat atgggccaca
      421 tccatgagta aatcagttaa ttttattctt ggtagcatct gataaaggct tgcactaaat
      481 ttttttgctt cttctggaac atctttttct aagcgtgcaa gtgatagctt tcctttttca
      541 agagaaaccc catctaactt attggaattg gcagctaacc actttaacct ttcattaaag
      601 ctgctggttc tctccgttat ataatcttcg aatgataaac taactgataa tctcgtattc
      661 cccttcgatt gattccatgt atcttccgaa aacaaatatt cctcaaaatc cctatattgt
      721 ctgctgccaa caatggaaac atctcctgcc cgaacatgct cccgaagttc tgttaaaaca
      781 gccatttcat agtaatgacg attaattgtt gtaccatcat cctcgtataa atgtcttttc
      841 catcgttttg aaataaaatc cacaggtgag tcatcaggca cttttcgctt tccagattcg
      901 ttcattcctc ggataatctc aacagcttgt aaaagtggct catttgcctt tgtagaatga
      961 aattccaata ctcttaatag cgttggcgta tattttctta gtgaataaaa ccgtttttgc
     1021 agtaagtcta aataatcata gtcggcagga cgtgcaagtt cctgagcctc ttctactgaa
     1081 gagacaaagg tattccattc aataaccgat tctaaaacct taaaaacgtc taatttttcc
     1141 tctcttgctt taattaatgc ttgtccgatg ttcgtaaagt gtataacttt ctcatttagc
     1201 tttttaccgt tttgtttctg gatttcctct tgagccttac gaccttttga taacaaacta
     1261 agtatttgcc tatcatgaat ttcaaacgct ttatccgtta gctcctgagt aagttgtaat
     1321 aaatagatgg ttaatatcga ataacgttta ttttcttgaa agtcacggaa tgcatacggc
     1381 tcgtatcttg agcctaagcg agacagctgc aacaggcggt tacggtgcaa atgactaatt
     1441 tgcactgttt ctaaatccat tcctcgtatg tattcgagtc gttctattat ttttagaaaa
     1501 gtttcgggtg aaggatgacc cggtggctct tttaaccaac ccaatatcgt tttattggat
     1561 tcggatggat gctgcgaggt aataatccct tcaagctttt ctttttgctc atttgttaga
     1621 gatttactaa ccgtattaaa tagcttcttt tcagccattg cccttgcttc ccacaccatt
     1681 ctttcaagtg tagtgatagc aggcagtata attttgtttt ttcttagaaa atctatgcat
     1741 tcatgcagta gatgaatggc atcaccattt tccaaagcta attgatgaag gtacttaaat
     1801 gtcattcgat attcactcag ggtaaaagtt acaaagtcgt attcacttcg aatttctttc
     1861 aaatgatccc aaagtgtatt ttccctttga ggataatgat caagcgagga tggactaaca
     1921 ccaatctgtt tcgatatata ttgtatgacc gaatctggga tgcttttgat atgagtgtat
     1981 ggccaaccgg gataccgaag aacagctaat tgaacagcaa atcctaaacg gttttcttcc
     2041 ctccttcgct tattaactat ttctaaatcc cgtttggaaa aagtgaagta ggtccccagt
     2101 atccattcat cttcagggat ttgcataaaa gcctgtctct gttccggtgt aagcaattct
     2161 ctacctctcg caattttcat tcagtatcat tccatttctg tattttcaat ttattagttc
     2221 aattatatat caatagagtg tactctattg atacaaatgt agtagactga taaaatcata
     2281 gttaagagcg tctcataaga cttgtctcaa aaatgaggtg atattttgcg gaaaatcggt
     2341 tatattcgtg tcagttcgac taaccagaat ccttcaagac aatttcagca gttgaacgag
     2401 atcggaatgg atattatata tgaagagaaa gtttcaggag caacaaagga tcgcgagcaa
     2461 cttcaaaaag tgttagacga tttacaggaa gatgacatca tttatgttac agacttaact
     2521 cgaatcactc gtagtacaca agatctattt gaattaatcg ataacatacg agataaaaag
     2581 gcaagtttaa aatcactaaa agatacatgg cttgatttat cagaagataa tccatacagc
     2641 caattcttaa ttactgtaat ggctggtgtt aaccaattag agcgagatct tattcggatg
     2701 agacaacgtg aagggattga attggctaag aaagaaggaa agtttaaagg tcgattaaag
     2761 aagtatcata aaaatcacgc aggaatgaat tatgcggtaa agctatataa agaaggaaat
     2821 atgactgtaa atcaaatttg tgaaattact aatgtatcta gggcttcatt atacaggaaa
     2881 ttatcagaag tgaataatta gccattctgt attccgctaa tgggcaatat ttttaaagaa
     2941 gaaaaggaaa ctataaaata ttaacagcct cctagcgatg ccgaaaagcc ctttgataaa
     3001 aaaagaatca tcatcttaag aaattcttag tcatttatta tgtaaatgct tataaattcg
     3061 gccctataat ctgataaatt attaagggca aacttatgtg aaagggtgat aactatgagc
     3121 gataaaatac ttattgtgga tgatgaacat gaaattgccg atttggttga attatactta
     3181 aaaaacgaga attatacggt tttcaaatac tataccgcca aagaagcatt ggaatgtata
     3241 gacaagtctg agattgacct tgccatattg gacatcatgc ttcccggcac aagcggcctt
     3301 actatctgtc aaaaaataag ggacaagcac acctatccga ttatcatgct gaccgggaaa
     3361 gatacagagg tagataaaat tacagggtta acaatcggcg cggatgatta tataacgaag
     3421 ccctttcgcc cactggagtt aattgctcgg gtaaaggccc agttgcgccg atacaaaaaa
     3481 ttcagtggag taaaggagca gaacgaaaat gttatcgtcc actccggcct tgtcattaat
     3541 gttaacaccc atgagtgtta tctgaacgag aagcagttat cccttactcc caccgagttt
     3601 tcaatactgc gaatcctctg tgaaaacaag gggaatgtgg ttagctccga gctgctattt
     3661 catgagatat ggggcgacga atatttcagc aagagcaaca acaccatcac cgtgcatatc
     3721 cggcatttgc gcgaaaaaat gaacgacacc attgataatc cgaaatatat aaaaacggta
     3781 tggggggttg gttataaaat tgaaaaataa aaaaaacgac tattccaaac tagaacgaaa
     3841 actttacatg tatatcgttg caattgttgt ggtagcaatt gtattcgtgt tgtatattcg
     3901 ttcaatgatc cgagggaaac ttggggattg gatcttaagt attttggaaa acaaatatga
     3961 cttaaatcac ctggacgcga tgaaattata tcaatattcc atacggaaca atatagatat
     4021 ctttatttat gtggcgattg tcattagtat tcttattcta tgtcgcgtca tgctttcaaa
     4081 attcgcaaaa tactttgacg agataaatac cggcattgat gtacttattc agaacgaaga
     4141 taaacaaatt gagctttctg cggaaatgga tgttatggaa caaaagctca acacattaaa
     4201 acggactctg gaaaagcgag agcaggatgc aaagctggcc gaacaaagaa aaaatgacgt
     4261 tgttatgtac ttggcgcacg atattaaaac gccccttaca tccattatcg gttatttgag
     4321 cctgcttgac gaggctccag acatgccggt agatcaaaag gcaaagtatg tgcatatcac
     4381 gttggacaaa gcgtatcgac tcgaacagct aatcgacgag ttttttgaga ttacacggta
     4441 taacctacaa acgataacgc taacaaaaac gcacatagac ctatactata tgctggtgca
     4501 gatgaccgat gaattttatc ctcagctttc cgcacatgga aaacaggcgg ttattcacgc
     4561 ccccgaggat ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct ttaacaacat
     4621 tttgaaaaac gccgctgcat acagtgagga taacagcatc attgacatta ccgcgggcct
     4681 ctccggggat gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa aagataagct
     4741 agctgccata tttgaaaagt tctataggct ggacaatgct cgttcttccg atacgggtgg
     4801 cgcgggactt ggattggcga ttgcaaaaga aattattgtt cagcatggag ggcagattta
     4861 cgcggaaagc aatgataact atacgacgtt tagggtagag cttccagcga tgccagactt
     4921 ggttgataaa aggaggtcct aagagatgta tataattttg gctctttgtc aaatgatgtt
     4981 ggtaaagaga aatct
//
LOCUS       DACEQH010000245         4864 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.guided.112, whole genome shotgun sequence.
ACCESSION   DACEQH010000245 DACEQH010000000
VERSION     DACEQH010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.guided.112"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            363..1274
                     /locus_tag="IW833_002940"
     CDS             363..1274
                     /locus_tag="IW833_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ1308955.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1427..1912
                     /gene="vanZ"
                     /locus_tag="IW833_002941"
     CDS             1427..1912
                     /gene="vanZ"
                     /locus_tag="IW833_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1308956.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2199..2528)
                     /locus_tag="IW833_002942"
     CDS             complement(2199..2528)
                     /locus_tag="IW833_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1308957.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2833..3162
                     /locus_tag="IW833_002943"
     CDS             2833..3162
                     /locus_tag="IW833_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1308958.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            3152..3439
                     /locus_tag="IW833_002944"
     CDS             3152..3439
                     /locus_tag="IW833_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1308959.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3718..4281)
                     /locus_tag="IW833_002945"
     CDS             complement(3718..4281)
                     /locus_tag="IW833_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1308960.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         1705 a          734 c          837 g         1588 t
ORIGIN      
        1 tatttgatag tagatttaaa actttgcaac agaaccattg aagatgtaag gcagagcgat
       61 attgcggtca ttatctgcgt gcgctgcggc aagatagcct gataataaga ctgatcgcat
      121 agaggggtgg tatttcacac cgcccattgt caacaggcag ttcagcctcg ttaaattcag
      181 catgggtatc acttatgaaa attcatctac attggtgata atagtaaatc cagtagggcg
      241 aaataattga ctgtaattta cggggcaaaa cggcacaatc tcaaacgaga ttgtgccgtt
      301 taaggggaag attctagaaa tatttcatac ttccaactat atagttaagg aggagactga
      361 aaatgaagaa gttgtttttt ttattgttat tgttattctt aatatactta ggttatgact
      421 acgttaatga agcactgttt tctcaggaaa aagtcgaatt tcaaaattat gatcaaaatc
      481 ccaaagaaca tttagaaaat agtgggactt ctgaaaatac ccaagagaaa acaattacag
      541 aagaacaggt ttatcaagga aatctgctat taatcaatag taaatatcct gttcgccaag
      601 aaagtgtgaa gtcagatatc gtgaatttat ctaaacatga cgaattaata aatggatacg
      661 ggttgcttga tagtaatatt tatatgtcaa aagaaatagc acaaaaattt tcagagatgg
      721 tcaatgatgc tgtaaagggt ggcgttagtc attttattat taatagtggc tatcgagact
      781 ttgatgagca aagtgtgctt taccaagaaa tgggggctga gtatgcctta ccagcaggtt
      841 atagtgagca taattcaggt ttatcactag atgtaggatc aagcttgacg aaaatggaac
      901 gagcccctga aggaaagtgg atagaagaaa atgcttggaa atacgggttc attttacgtt
      961 atccagagga caaaacagag ttaacaggaa ttcaatatga accatggcat attcgctatg
     1021 ttggtttacc acatagtgcg attatgaaag aaaagaattt cgttctcgag gaatatatgg
     1081 attacctaaa agaagaaaaa accatttctg ttagtgtaaa tggggaaaaa tatgagatct
     1141 tttattatcc tgttactaaa aataccacca ttcatgtgcc gactaatctt cgttatgaga
     1201 tatcaggaaa caatatagac ggtgtaattg tgacagtgtt tcccggatca acacatacta
     1261 attcaaggag gtaaggatgg cggaatgaaa ccaacgaaat taatgaacag cattattgta
     1321 ctagcacttt tggggtaacg ttagcttttt aatttaaaac ccacgttaac taggacattg
     1381 ctatactaat gatacaactt aaacaaaaga attagaggaa attatattgg gaaaaatatt
     1441 atctagagga ttgctagctt tatatttagt gacactaatc tggttagtgt tattcaaatt
     1501 acaatacaat attttatcag tatttaatta tcatcaaaga agtcttaact tgactccatt
     1561 tactgctact gggaatttca gagagatgat agataatgtt ataatcttta ttccatttgg
     1621 cttgcttttg aatgtcaatt ttaaagaaat cggattttta cctaagtttg cttttgtact
     1681 ggttttaagt cttacttttg aaataattca atttatcttc gctattggag cgacagacat
     1741 aacagatgta attacaaata ctgttggagg ctttcttgga ctgaaattat atggtttaag
     1801 caataagcat atgaatcaaa aaaaattaga cagagttatt atttttgtag gtatactttt
     1861 gctcgtatta ttgctcgttt accgtaccca tttaagaata aattacgtgt aagatgtcta
     1921 aatcaagcaa tctgatcttt catacacata aagatattga atgaattgga ttagatggaa
     1981 aacgggatgt ggggaaactc gcccgtaggt gtgaagtgag gggaaaaccg gtgataaagt
     2041 aaaaagctta cctaacacta tagtaacaaa gaaagcccaa ttatcaattt tagtgctgag
     2101 gaattggtct ctttaataaa tttccttaac gttgtaaatc cgcattttcc tgacggtacc
     2161 cccaaactaa gatttcatag atagattttc tgataacttt aaaaaacacg aatagcggaa
     2221 aggatgtctt cattcgctaa agagtaataa actacttttc catcttgacg tgattttgcc
     2281 attccatgtt tttttaaaaa acgtaaatgg tgtgacgttg tagcgacact agcagtaagt
     2341 atttctgcta aatcacagac gcacatttcc ttataggttt ctaaagcata gaaaatttta
     2401 attctagaaa aatcgctgaa acatttccct aacacaagta agtttgaaaa atctttttga
     2461 tccaatttat tttttattcc atttactttt tctttatgta cgacagtcac ttggcaaact
     2521 tttttcattc atctcacctc acattcaaac gattatttga atgaagtata cctatttgat
     2581 cactcttttg tcaatctacc ttaattccaa tacactatta taagcaattt catgtattga
     2641 agtaaactta cttgcaagtg cgtttttgat tccaagttaa aaacatatta ttattctaca
     2701 taatttccta atcaaataaa actcctttga aaattgttct aaaggagttt tattattttg
     2761 tatttctact aaaaatagcg tacaatgtaa ttactaagta attacgaaat gtaaaataat
     2821 ggaggaaata atatgattac aaaaacaaga aaacaaggaa attctattat gttaacggtc
     2881 cctaaagaat tcgatgtgcc caatggtgtt gaagtagaag ctaagcttgt agagaatggt
     2941 atcctttatg agtttgtaga gcctaaaaaa gaattttttg attttagtga agatattttg
     3001 gctgatattc tttcagaagg ttatagcaaa caagagatct taaaagaatt tagaaataga
     3061 aaaaatgcgt taacttctgc atttaaatct attgctgaag atactgttgc aaattctaaa
     3121 ccgatgacaa aagaggagtt ggcagcagaa attggcttat gagattctac cttctaaaca
     3181 cgtaataaag tatctaaaaa aattaaaaga gaaaacatta aaagaacaat ttttaacgat
     3241 aatttatgat gagatagcag ttcgtcctca ttcgggagaa caaaaaacgg gtgatttatc
     3301 aggaatttgg gctattggat tcaagtatgc aggtacgaca tatcgcgttg catatgaaat
     3361 taaagataat gcagtgatac ctatcttact atgtggaacg catgaaaatt tttatgaaca
     3421 attaaaaaag attaggtaag aacccaatgt ttcaacttga gaggatagac gacactttaa
     3481 aagataattt ttaacagctg agagttgtaa taccctataa ttttaaacca ctaacctata
     3541 tatttatgaa aaatagaaaa aattaaaacg cattttaagc ctctttcaaa ttaaaagctt
     3601 catccttatg cacaaagagg gcttaaaatg cgttatttta tattgttttt tagtaataat
     3661 aaaaaaatca ctctaattct cccataaata ttatagattc ctaaagatta aaatagatta
     3721 ttccctattg tcttttaagt agcggtagag tgttgccttg ctaataccag tcatttccgt
     3781 aatttctttt acgctataaa cctgactttt atacaatttt actgctcgat caacatcttt
     3841 ctggttcact cgtggacggc cacctttacg acctctcgca cgtgcacttt tcaaaccttc
     3901 tttggtacgt tcaacaatta aatcacgttc gaactgacta aatgcttgga agacagtcat
     3961 cattaagcgt ccgtgaggcg tgctggtatc gaagttttct ttcaagctca ccagcttaac
     4021 atggtgttct tcaaaatagg taacaaggtc aatcaaatcc tttgtactac gacccaatcg
     4081 agaaaaactt tctacaatta ccgtatctcc cggacgaatt ttatctttta aacgatttag
     4141 ttggggacga accgctttgg ttcccgtcat tttctcagtt aggatttcat tacaattttg
     4201 ttcattgagt aagtctaatt gacgatttag ttcttgtgaa cgtgtactca ctcgggcata
     4261 accataaatg tatccactca tgcttttctt cctttcgtac tttaattctg gcttgattgt
     4321 atcataaaag ggagttatag atacatagtt aatgcaacaa gttatgatac aaaatatgac
     4381 ctcttttatt taataaaaga ggtcatttca ttttgtctca taatcgattg tttttgatac
     4441 gagtgtgtat acgaaataaa caaaaaaatc ttaagtaaat ttctagatac ctaatttaat
     4501 cctaactagt gtatcctaaa gttaataacg agtttatcac aatgaaaatt ataaaatgct
     4561 cccatattag atataacatg caatatttga attagaacta caaaatttat tcgttttata
     4621 aagtaaataa tgttgagata ctaaggaata gaatattata aggaattgag ctgaatgtat
     4681 tggataaaaa aagaataact ttattagaga tggactataa aaatgctcgt aattttcttc
     4741 tgaagtctga aagctatttg aatgctcagc taccagaata tattagtttt gacgaaactt
     4801 taaaatctgc taaaaaagtt ttaacttcgc cgtttgtata attaagcgag acaaataaaa
     4861 aagc
//
LOCUS       DACEQH010000246         4328 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.guided.117, whole genome shotgun sequence.
ACCESSION   DACEQH010000246 DACEQH010000000
VERSION     DACEQH010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.guided.117"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            279..1757
                     /gene="msr"
                     /locus_tag="IW833_002946"
     CDS             279..1757
                     /gene="msr"
                     /locus_tag="IW833_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1308961.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1804..2250
                     /locus_tag="IW833_002947"
     CDS             1804..2250
                     /locus_tag="IW833_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1308962.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2449..2964)
                     /locus_tag="IW833_002948"
     CDS             complement(2449..2964)
                     /locus_tag="IW833_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1308963.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3081..4282
                     /locus_tag="IW833_002949"
                     /pseudo
     CDS             3081..4282
                     /locus_tag="IW833_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         1301 a          728 c          935 g         1364 t
ORIGIN      
        1 gaaaaatgac tgcatcgatg aaattacgtt tcgaactttt gaataacaac taaataaatt
       61 caaaagacat gtataaacaa caatttcatg gatggagtat gtccattttt tcgaacgaca
      121 gtagaatagg tgaaagctat gacagatttg gtgatttagt catagttatt tcgttcagag
      181 taggaaaaga tgtgatgcag tttttttgca aacagatttt tctgctcttt tttagtatcg
      241 ataattgttg ttttacatga aataggagtg taaaacatat ggaaaattta gcagtaaata
      301 taacaaatct acaagtcagc tttggcaacc agctagaact atctattgat tctcttcgtg
      361 tctatcagca agatcgtata gggatcatag gagaaaatgg tgtcggtaaa tcgactttgc
      421 tcaaactaat agccggtgaa ctttttcccg atcatgggaa aatccaaaca gagatcacct
      481 tcaactacct gcctcaatta acctatctcg ctgaagcaaa ggacctaaat ttggaattag
      541 ccagtcattt ccagttaaga ctggaagaaa cttcagagcg gaaatggagc ggaggggaag
      601 aacgaaagat cgagttgata cgtcttcttt cttcttatga acaagggatg cttctagacg
      661 agccaacaac ccatctagat agaaaaagta ttgatcgact gattgaagag cttcgttatt
      721 attatggtac gctggttttt gttagtcatg atcgctattt tctagatgag ctagcatcga
      781 aaatctggga agtaaaagac ggagaaatcc gagagttttc ggggaattat agtgcctatc
      841 tcactcaaaa ggaattggag aaaaagactc agctacgaga agcagagtcg atcatgaaag
      901 agaaaaaacg attggaaaaa tcgatccaag aaaagaaaaa acaagcggaa aagttggaaa
      961 aagtgtccag taaaaagaaa aagcaacaaa tcagaccgga tcggttgtct tcctctaaac
     1021 aaaaagacag tgtacaaaaa gccatccaaa agaatgcgaa aacattagag agaagactcc
     1081 aaaaaatagg agaaacaacc aagccgcagc agatgaaaca aatccgtttt ccagtaccaa
     1141 aatctcttga actccacagc cgctatccca tcatgggaca aaatgtccaa ttggaaagaa
     1201 gtgggagaac attactggta aatggtgatt ttcagttttc tttaggtaaa aaaatcgcga
     1261 ttgtcggtga aaatggttca ggtaagacaa ccttattaga acatatccgc aaacaaggag
     1321 aaggaatcct tctctctccg aaagtaagct ttcaagtata tcagcaaaag ggttatcaaa
     1381 tgacatctga agaatccatc attcgttttg tcatgagaca aacagagttt tcagaatcgc
     1441 ttgtccgtag tttgctgaat cacttagggt ttgctcagga aactttgacg aaaccgttat
     1501 gtacattaag tgggggagaa gcgacccgtc tgacgattgc tttgcttttt actaagccaa
     1561 gtaatgtgtt gctgttagat gaaccgacta attttattga tatggcaacg atcgaagctt
     1621 tagagaagct aatgcaaata tatccgggaa cgattttgtt tacttctcat gattcttact
     1681 ttgtcgagcg tacggctgat gaagtttatg aaataaaagg gcagaaaata aaaaaagtac
     1741 ttacgagaaa tttttaaatg tgctacgatg attttcgtga caaatttaaa ggagtggtac
     1801 agattgccta caaataaaaa agaaggaata atttttacaa caatgatgtg ttttttgatg
     1861 gtcttgggga tgagcgcgta taatttatgg ctgcatcaag atcttcattt atcaagtttg
     1921 ttgattggtc tagtgcctgg attcgtggtg gctttcattt tggatgtctt catcatcggt
     1981 gtgctggcga aaaaaattgc ctttaaacta ccagtaaatc cagaaagcaa ggtccaaatg
     2041 atcttaacta tttcatgctt gatggtttta ggaatggtga catgtatgtc tttattcggt
     2101 gtgatcatgg aacaaggaat acctgatcaa ttattctctg cttatatgaa tgcttggaaa
     2161 atgaatgtga ttgccgcttt accgttgcag ttgatcatcg ttggcccgct ttcaagaagt
     2221 gtgctaaaag caattcaaag taatgaatga tggaacactg ttatttgaaa aaaggccttg
     2281 gcatgctttt tgtcatagtc tcttgaatgc agttctagaa aaatagtgat ccgaacgata
     2341 ggttttggcc tgtcgttcgg atcgcttttt cttttccaac tgtattgctc ttttattgaa
     2401 gataatttgc tcttcaatag atatttctag aaaggttctg tttatccctt actcaaaaaa
     2461 ctgctgcatt tgttctctta cttgctgaca ccattggagt tcctgctgac aaagcataat
     2521 tctgcgctgg atcacaacat aacgtccaaa ttcttgtttc ttgtctagcg agaggtcatt
     2581 caattttttt tgattttttt gcatgatttt gttcagctgt tgttctctag tgaatagcag
     2641 tccagtagct gttgttttat cgagcgtcga aaaagcatag atttttgcta gaaattcatc
     2701 tttttgcgta ggagcgggag tttcctcttt gatccattcc tctacaagag cacgtccttc
     2761 atcagtcaag tggtagatct ttttttgaga gtgctcgtct ttgtccaaaa cggtcacata
     2821 accctgctgg tctaacttgg caagcgttgt atagatctga ctgtgatggg cttcccaaaa
     2881 aattgtgatc agctgcttca attcataccc agaacgtggt ttggataaaa gcacgcataa
     2941 taatatatag gctaaagtat tcataaaaat cctacctctc gtcttatttt ctttattata
     3001 tcacagtaaa gtttccacgt tttacatgtg catatttaca tgtaaaataa atcatgtatg
     3061 atgctaatag agagaaaggg atggaaatat tggagaatca aatagatata caagaacaaa
     3121 caaatgaaga aacaaagggg aaaagttcag cgctagttgt agcagctacc tgtatgatcg
     3181 cttttatggg agtgggtcta gtagacccaa tcttaaaaac gattgctgca cagttaaacg
     3241 ctacgccagc tgaaacgacc ctgttattta ctagttacat gttagtcact ggtgtggtga
     3301 tgctatttac aggtttctta tctagtcgga tcggtttgaa aaaacattga tgacaggatt
     3361 gtttatcatc gttgtattcg ctggtcttgg cggattatct gggaatatcg gaatgctgat
     3421 tgggttacgt gccggctggg gtgtcgggaa tgctttattt gtctctactg ctttagcaac
     3481 gatcgtcagt attttagttg gtaacacaga aaaagcaatc atgatgtatg aagcagcact
     3541 tggctgcgga atggctgttg gccctctagt cggtggggtc ttgggcagtg gttcatggcg
     3601 ttatccattt tttggggtag catttttgat gttttgcggt tttatagccc tagcggtact
     3661 tttacctgaa actgagaaac caaagagaaa agtaggcctt tgggaaggtg tcaaagcgtt
     3721 aagcaatcat aaacttcgtt cagttggact aatcgcattg ttatataatt tcggattctt
     3781 tactttactt gcgtatgcgc catttttatt gattggctat tctgaattag aagtcggttt
     3841 tgtatttttt ggctggggtg tgcttctggc tattgcttcg attttcgttg caccggctct
     3901 tgagcggaag tttacgactt atcggacaat tttagcagca tttgtcttat tcattatttg
     3961 tttgctgtta ttaggagctg gaacttctat gccagtcctt gtaccgatca gcattgtgtt
     4021 agcaggattt ttccaaggaa ttatcaatac actgttgacg acgattgcta tggaaatacc
     4081 aggtctgcaa cgaaacgttg cttcgtccag ctatagtttt gtccgctttt tcggtggagc
     4141 tttagcacca tttattgcag gtgtgattgg tgaacatatt ggtgaaagct atgcgtttta
     4201 ttttgcaggt gccattgttc ttatcagtgt atgttgtatc atttatcacc gtaattattt
     4261 tgctacaaat gaagaagaat aagaaatcag aggttgtgaa aaagctgtcc tgcatcaagt
     4321 cataagaa
//
LOCUS       DACEQH010000247         3036 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.guided.136, whole genome shotgun sequence.
ACCESSION   DACEQH010000247 DACEQH010000000
VERSION     DACEQH010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.guided.136"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            245..1213
                     /locus_tag="IW833_002950"
     CDS             245..1213
                     /locus_tag="IW833_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1308964.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="IW833_002951"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="IW833_002951"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1308965.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="IW833_002952"
     CDS             2243..2851
                     /locus_tag="IW833_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1308966.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          904 a          581 c          729 g          822 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga ggttctgttg caaagtttta aataaagaat aaaatctccc
     3001 ttacggtatc tatgatttaa gctgggattc ccaata
//
LOCUS       DACEQH010000248          885 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E286
            SAMN07141395-rid9869903.guided.197, whole genome shotgun sequence.
ACCESSION   DACEQH010000248 DACEQH010000000
VERSION     DACEQH010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141395
            Sequence Read Archive: SRR7211837
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740669.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740669.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:53:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,953
            CDSs (total)                      :: 2,886
            Genes (coding)                    :: 2,814
            CDSs (with protein)               :: 2,814
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 36 of 72
            Pseudo Genes (incomplete)         :: 30 of 72
            Pseudo Genes (internal stop)      :: 19 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141395-rid9869903.guided.197"
                     /isolate="E286"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Feb-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            439..>885
                     /gene="dfrF"
                     /locus_tag="IW833_002953"
     CDS             439..>885
                     /gene="dfrF"
                     /locus_tag="IW833_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ1308967.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYT"
BASE COUNT          319 a          105 c          196 g          265 t
ORIGIN      
        1 tgaaaggatg ggaaaaaggt gtttcccaaa caaaaagata ttaagaaaca gcccatgttt
       61 acctgcttgc aaacaaaaga atattgctac tagactaaga gatactacat aagtgtgttg
      121 gtatctcttt tttgttgccg tgaaacagca gactatttca tttctgcttg attaccttaa
      181 aacttattag tctatggttt gtcaagggct tgttgcgtga gaaatgctga atatcaccct
      241 ttacaaaaca taggctgata agtaactttt caaaagcaga aaagaaatgg caatgcttat
      301 aacaatatga tataatgcag atgaagacaa aaaaggaatt tgtcgagctg gttagttcga
      361 gataattttg agtttgttaa gatgtggtgc acttgatgga atcacaggta cgacatcata
      421 ttatggagga tgaaatagat gataggtttg attgttgcga ggtcaaagaa taatgttata
      481 ggcaagaatg gtaatatacc atggaaaata aagggagaac aaaagcaatt tagagagtta
      541 acaacgggta atgtggttat tatggggcga aagtcttatg aagaaatcgg tcatccgttg
      601 cctaatagaa tgaatattgt tgtttccacc acaacagagt atcaaggaga taatttagtt
      661 tcagttaaat cattagaaga tgcattatta ttggctaaag gacgagatgt atacatatct
      721 ggtggatatg gactatttaa ggaagctttg caaatagtag ataaaatgta tatcacagaa
      781 gtagatttaa atattgaaga tggagataca ttctttccag aatttgatat caatgatttt
      841 gaagttttga taggggaaac acttggtgag gaagtgaaat atacg
//
